LOCUS       DAYYFU010000001       129000 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000001 DAYYFU010000000
VERSION     DAYYFU010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 129000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 129000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..129000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.001"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..1535)
                     /locus_tag="ACHA34_000001"
     CDS             complement(12..1535)
                     /locus_tag="ACHA34_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM8790688.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="ACHA34_000002"
     CDS             1787..2968
                     /locus_tag="ACHA34_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8790689.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="ACHA34_000003"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="ACHA34_000003"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFM8790690.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="ACHA34_000004"
     CDS             complement(5053..7764)
                     /locus_tag="ACHA34_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM8790691.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="ACHA34_000005"
     CDS             complement(7774..8475)
                     /locus_tag="ACHA34_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8790692.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="ACHA34_000006"
     CDS             8661..9260
                     /locus_tag="ACHA34_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8790693.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="ACHA34_000007"
     CDS             9341..10354
                     /locus_tag="ACHA34_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFM8790694.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="ACHA34_000008"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="ACHA34_000008"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFM8790695.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="ACHA34_000009"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="ACHA34_000009"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFM8790696.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="ACHA34_000010"
     CDS             11802..13175
                     /locus_tag="ACHA34_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8790697.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="ACHA34_000011"
     CDS             13532..15676
                     /locus_tag="ACHA34_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8790698.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(15728..16768)
                     /locus_tag="ACHA34_000012"
     CDS             complement(15728..16768)
                     /locus_tag="ACHA34_000012"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8790699.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17045..18847)
                     /locus_tag="ACHA34_000013"
     CDS             complement(17045..18847)
                     /locus_tag="ACHA34_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8790700.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18861..19613)
                     /locus_tag="ACHA34_000014"
     CDS             complement(18861..19613)
                     /locus_tag="ACHA34_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8790701.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19622..19984)
                     /locus_tag="ACHA34_000015"
     CDS             complement(19622..19984)
                     /locus_tag="ACHA34_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8790702.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20160..21032)
                     /locus_tag="ACHA34_000016"
     CDS             complement(20160..21032)
                     /locus_tag="ACHA34_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8790703.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21058..21570)
                     /locus_tag="ACHA34_000017"
     CDS             complement(21058..21570)
                     /locus_tag="ACHA34_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8790704.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(21708..22499)
                     /locus_tag="ACHA34_000018"
     CDS             complement(21708..22499)
                     /locus_tag="ACHA34_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFM8790705.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22554..23162)
                     /locus_tag="ACHA34_000019"
     CDS             complement(22554..23162)
                     /locus_tag="ACHA34_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFM8790706.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23155..23472)
                     /locus_tag="ACHA34_000020"
     CDS             complement(23155..23472)
                     /locus_tag="ACHA34_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8790707.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23628..24356)
                     /locus_tag="ACHA34_000021"
     CDS             complement(23628..24356)
                     /locus_tag="ACHA34_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFM8790708.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24515..25396
                     /locus_tag="ACHA34_000022"
     CDS             24515..25396
                     /locus_tag="ACHA34_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8790709.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25633..26676)
                     /locus_tag="ACHA34_000023"
     CDS             complement(25633..26676)
                     /locus_tag="ACHA34_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8790710.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26679..27347)
                     /locus_tag="ACHA34_000024"
     CDS             complement(26679..27347)
                     /locus_tag="ACHA34_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8790711.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27489..29825
                     /locus_tag="ACHA34_000025"
     CDS             27489..29825
                     /locus_tag="ACHA34_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFM8790712.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29940..30251)
                     /locus_tag="ACHA34_000026"
     CDS             complement(29940..30251)
                     /locus_tag="ACHA34_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8790713.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30432..31907
                     /locus_tag="ACHA34_000027"
     CDS             30432..31907
                     /locus_tag="ACHA34_000027"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFM8790714.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31954..32538
                     /locus_tag="ACHA34_000028"
     CDS             31954..32538
                     /locus_tag="ACHA34_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFM8790715.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            32540..32959
                     /locus_tag="ACHA34_000029"
     CDS             32540..32959
                     /locus_tag="ACHA34_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFM8790716.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(33240..33524)
                     /locus_tag="ACHA34_000030"
     CDS             complement(33240..33524)
                     /locus_tag="ACHA34_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFM8790717.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33876..34586)
                     /locus_tag="ACHA34_000031"
     CDS             complement(33876..34586)
                     /locus_tag="ACHA34_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFM8790718.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34583..36055)
                     /locus_tag="ACHA34_000032"
     CDS             complement(34583..36055)
                     /locus_tag="ACHA34_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8790719.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36193..36666
                     /locus_tag="ACHA34_000033"
     CDS             36193..36666
                     /locus_tag="ACHA34_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8790720.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36819..37370)
                     /locus_tag="ACHA34_000034"
     CDS             complement(36819..37370)
                     /locus_tag="ACHA34_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8790721.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37475..38005
                     /locus_tag="ACHA34_000035"
     CDS             37475..38005
                     /locus_tag="ACHA34_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFM8790722.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38488..38931)
                     /locus_tag="ACHA34_000036"
     CDS             complement(38488..38931)
                     /locus_tag="ACHA34_000036"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8790723.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38947..40107)
                     /locus_tag="ACHA34_000037"
     CDS             complement(38947..40107)
                     /locus_tag="ACHA34_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8790724.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40614..42314
                     /locus_tag="ACHA34_000038"
     CDS             40614..42314
                     /locus_tag="ACHA34_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8790725.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42416..43249)
                     /locus_tag="ACHA34_000039"
     CDS             complement(42416..43249)
                     /locus_tag="ACHA34_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFM8790726.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43553..45718)
                     /locus_tag="ACHA34_000040"
     CDS             complement(43553..45718)
                     /locus_tag="ACHA34_000040"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFM8790727.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45978..48272
                     /locus_tag="ACHA34_000041"
     CDS             45978..48272
                     /locus_tag="ACHA34_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFM8790728.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48265..48930
                     /gene="pgmB"
                     /locus_tag="ACHA34_000042"
     CDS             48265..48930
                     /gene="pgmB"
                     /locus_tag="ACHA34_000042"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8790729.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            48923..49930
                     /locus_tag="ACHA34_000043"
     CDS             48923..49930
                     /locus_tag="ACHA34_000043"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8790730.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49952..50971
                     /locus_tag="ACHA34_000044"
     CDS             49952..50971
                     /locus_tag="ACHA34_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8790731.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="ACHA34_000045"
     CDS             complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="ACHA34_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8790732.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52729..54150)
                     /gene="tyrP"
                     /locus_tag="ACHA34_000046"
     CDS             complement(52729..54150)
                     /gene="tyrP"
                     /locus_tag="ACHA34_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFM8790733.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="ACHA34_000047"
     CDS             complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="ACHA34_000047"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8790734.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56561..57817)
                     /gene="tyrS"
                     /locus_tag="ACHA34_000048"
     CDS             complement(56561..57817)
                     /gene="tyrS"
                     /locus_tag="ACHA34_000048"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFM8790735.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57929..58134)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58397..59698
                     /locus_tag="ACHA34_000049"
     CDS             58397..59698
                     /locus_tag="ACHA34_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8790736.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59767..60366)
                     /locus_tag="ACHA34_000050"
     CDS             complement(59767..60366)
                     /locus_tag="ACHA34_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8790737.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            60620..61501
                     /locus_tag="ACHA34_000051"
     CDS             60620..61501
                     /locus_tag="ACHA34_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8790738.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            61514..63124
                     /locus_tag="ACHA34_000052"
     CDS             61514..63124
                     /locus_tag="ACHA34_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8790739.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(63201..63860)
                     /locus_tag="ACHA34_000053"
     CDS             complement(63201..63860)
                     /locus_tag="ACHA34_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8790740.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(64143..64808)
                     /locus_tag="ACHA34_000054"
     CDS             complement(64143..64808)
                     /locus_tag="ACHA34_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFM8790741.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="ACHA34_000055"
     CDS             complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="ACHA34_000055"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFM8790742.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(65588..66598)
                     /gene="dhaK"
                     /locus_tag="ACHA34_000056"
     CDS             complement(65588..66598)
                     /gene="dhaK"
                     /locus_tag="ACHA34_000056"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFM8790743.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(66573..66968)
                     /gene="dhaM"
                     /locus_tag="ACHA34_000057"
     CDS             complement(66573..66968)
                     /gene="dhaM"
                     /locus_tag="ACHA34_000057"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFM8790744.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            67122..67442
                     /locus_tag="ACHA34_000058"
     CDS             67122..67442
                     /locus_tag="ACHA34_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFM8790745.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(67554..68411)
                     /locus_tag="ACHA34_000059"
     CDS             complement(67554..68411)
                     /locus_tag="ACHA34_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8790746.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(68519..69822)
                     /locus_tag="ACHA34_000060"
                     /pseudo
     CDS             complement(68519..69822)
                     /locus_tag="ACHA34_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(69819..70328)
                     /locus_tag="ACHA34_000061"
     CDS             complement(69819..70328)
                     /locus_tag="ACHA34_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8790747.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(70503..72638)
                     /locus_tag="ACHA34_000062"
     CDS             complement(70503..72638)
                     /locus_tag="ACHA34_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM8790748.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(72721..74376)
                     /locus_tag="ACHA34_000063"
     CDS             complement(72721..74376)
                     /locus_tag="ACHA34_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8790749.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(74378..74692)
                     /locus_tag="ACHA34_000064"
     CDS             complement(74378..74692)
                     /locus_tag="ACHA34_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8790750.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            74862..75122
                     /locus_tag="ACHA34_000065"
     CDS             74862..75122
                     /locus_tag="ACHA34_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFM8790751.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            75136..75450
                     /locus_tag="ACHA34_000066"
     CDS             75136..75450
                     /locus_tag="ACHA34_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFM8790752.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(76180..76734)
                     /locus_tag="ACHA34_000067"
     CDS             complement(76180..76734)
                     /locus_tag="ACHA34_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFM8790753.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(76860..78788)
                     /locus_tag="ACHA34_000068"
     CDS             complement(76860..78788)
                     /locus_tag="ACHA34_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM8790754.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(79094..79810)
                     /locus_tag="ACHA34_000069"
     CDS             complement(79094..79810)
                     /locus_tag="ACHA34_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8790755.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="ACHA34_000070"
     CDS             complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="ACHA34_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFM8790756.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="ACHA34_000071"
     CDS             complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="ACHA34_000071"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFM8790757.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(83280..84740)
                     /locus_tag="ACHA34_000072"
     CDS             complement(83280..84740)
                     /locus_tag="ACHA34_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8790758.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(84730..86025)
                     /locus_tag="ACHA34_000073"
     CDS             complement(84730..86025)
                     /locus_tag="ACHA34_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8790759.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(86357..87034)
                     /locus_tag="ACHA34_000074"
     CDS             complement(86357..87034)
                     /locus_tag="ACHA34_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFM8790760.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(87064..88155)
                     /locus_tag="ACHA34_000075"
     CDS             complement(87064..88155)
                     /locus_tag="ACHA34_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFM8790761.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(88256..88690)
                     /locus_tag="ACHA34_000076"
     CDS             complement(88256..88690)
                     /locus_tag="ACHA34_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8790762.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(89274..90731)
                     /locus_tag="ACHA34_000077"
     CDS             complement(89274..90731)
                     /locus_tag="ACHA34_000077"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8790763.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(90728..92623)
                     /locus_tag="ACHA34_000078"
     CDS             complement(90728..92623)
                     /locus_tag="ACHA34_000078"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8790764.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(92681..94456)
                     /locus_tag="ACHA34_000079"
     CDS             complement(92681..94456)
                     /locus_tag="ACHA34_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8790765.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(94506..96002)
                     /locus_tag="ACHA34_000080"
     CDS             complement(94506..96002)
                     /locus_tag="ACHA34_000080"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8790766.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(96196..97818)
                     /locus_tag="ACHA34_000081"
     CDS             complement(96196..97818)
                     /locus_tag="ACHA34_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8790767.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(98267..99580)
                     /locus_tag="ACHA34_000082"
     CDS             complement(98267..99580)
                     /locus_tag="ACHA34_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8790768.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(99825..100049)
                     /locus_tag="ACHA34_000083"
     CDS             complement(99825..100049)
                     /locus_tag="ACHA34_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8790769.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(100069..100491)
                     /locus_tag="ACHA34_000084"
     CDS             complement(100069..100491)
                     /locus_tag="ACHA34_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8790770.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(100635..101345)
                     /locus_tag="ACHA34_000085"
     CDS             complement(100635..101345)
                     /locus_tag="ACHA34_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFM8790771.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(101363..101860)
                     /locus_tag="ACHA34_000086"
     CDS             complement(101363..101860)
                     /locus_tag="ACHA34_000086"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8790772.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(101897..102349)
                     /locus_tag="ACHA34_000087"
     CDS             complement(101897..102349)
                     /locus_tag="ACHA34_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8790773.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(102484..103089)
                     /locus_tag="ACHA34_000088"
     CDS             complement(102484..103089)
                     /locus_tag="ACHA34_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8790774.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(103539..104159)
                     /locus_tag="ACHA34_000089"
     CDS             complement(103539..104159)
                     /locus_tag="ACHA34_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8790775.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(104161..105030)
                     /locus_tag="ACHA34_000090"
     CDS             complement(104161..105030)
                     /locus_tag="ACHA34_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8790776.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(105053..105460)
                     /locus_tag="ACHA34_000091"
     CDS             complement(105053..105460)
                     /locus_tag="ACHA34_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8790777.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(105479..106768)
                     /locus_tag="ACHA34_000092"
     CDS             complement(105479..106768)
                     /locus_tag="ACHA34_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8790778.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(106795..107097)
                     /locus_tag="ACHA34_000093"
     CDS             complement(106795..107097)
                     /locus_tag="ACHA34_000093"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8790779.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(107122..109215)
                     /locus_tag="ACHA34_000094"
     CDS             complement(107122..109215)
                     /locus_tag="ACHA34_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8790780.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(109704..110468)
                     /locus_tag="ACHA34_000095"
     CDS             complement(109704..110468)
                     /locus_tag="ACHA34_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFM8790781.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(110579..111448)
                     /gene="mdcB"
                     /locus_tag="ACHA34_000096"
     CDS             complement(110579..111448)
                     /gene="mdcB"
                     /locus_tag="ACHA34_000096"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFM8790782.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(111473..112549)
                     /locus_tag="ACHA34_000097"
     CDS             complement(111473..112549)
                     /locus_tag="ACHA34_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8790783.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(112573..113166)
                     /locus_tag="ACHA34_000098"
     CDS             complement(112573..113166)
                     /locus_tag="ACHA34_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFM8790784.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(113170..114846)
                     /gene="mdcD"
                     /locus_tag="ACHA34_000099"
     CDS             complement(113170..114846)
                     /gene="mdcD"
                     /locus_tag="ACHA34_000099"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFM8790785.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(114836..115135)
                     /locus_tag="ACHA34_000100"
     CDS             complement(114836..115135)
                     /locus_tag="ACHA34_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFM8790786.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(115237..116883)
                     /gene="mdcA"
                     /locus_tag="ACHA34_000101"
     CDS             complement(115237..116883)
                     /gene="mdcA"
                     /locus_tag="ACHA34_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFM8790787.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(116890..117801)
                     /locus_tag="ACHA34_000102"
     CDS             complement(116890..117801)
                     /locus_tag="ACHA34_000102"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8790788.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            117921..118778
                     /locus_tag="ACHA34_000103"
     CDS             117921..118778
                     /locus_tag="ACHA34_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8790789.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(118964..119326)
                     /locus_tag="ACHA34_000104"
     CDS             complement(118964..119326)
                     /locus_tag="ACHA34_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFM8790790.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(119515..119697)
                     /locus_tag="ACHA34_000105"
     CDS             complement(119515..119697)
                     /locus_tag="ACHA34_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8790791.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            119672..121111
                     /locus_tag="ACHA34_000106"
     CDS             119672..121111
                     /locus_tag="ACHA34_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8790792.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(121118..123670)
                     /locus_tag="ACHA34_000107"
     CDS             complement(121118..123670)
                     /locus_tag="ACHA34_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFM8790793.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(123784..125115)
                     /locus_tag="ACHA34_000108"
     CDS             complement(123784..125115)
                     /locus_tag="ACHA34_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8790794.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(125081..126559)
                     /locus_tag="ACHA34_000109"
     CDS             complement(125081..126559)
                     /locus_tag="ACHA34_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8790795.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(126864..128699)
                     /gene="tdc"
                     /locus_tag="ACHA34_000110"
     CDS             complement(126864..128699)
                     /gene="tdc"
                     /locus_tag="ACHA34_000110"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8790796.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        37756 a        26970 c        22549 g        41725 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15721 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15781 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15841 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15901 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15961 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    16021 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16081 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16141 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16201 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16261 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16321 gcgtataccg tacttctaca tctaaagttc caggaaatcc gtttcccata tctgggctag
    16381 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16441 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16501 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16561 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16621 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16681 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16741 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16801 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16861 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16921 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16981 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    17041 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17101 agatgaaata caatacgaag aagatcgtaa acggaaccca aatatctttg tatggatttt
    17161 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17221 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17281 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17341 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17401 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17461 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17521 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17581 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17641 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17701 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17761 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17821 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17881 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17941 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    18001 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18061 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18121 tgaaataagt tcctaatatg atcgtaacta aggctacccc tataccaaag acttgatagg
    18181 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18241 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18301 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18361 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18421 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18481 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18541 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18601 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18661 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18721 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18781 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18841 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18901 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18961 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    19021 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19081 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19141 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19201 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19261 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19321 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19381 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19441 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19501 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19561 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19621 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19681 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19741 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19801 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19861 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19921 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19981 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    20041 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20101 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20161 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20221 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20281 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20341 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20401 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20461 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20521 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20581 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20641 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20701 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20761 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20821 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20881 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20941 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    21001 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21061 ataaatatct aatgttactt ttttaatttt ttttcgaata cgcgccatta caccttctag
    21121 ttctaactct ttgtcttcag tgatgtacca accccttgcc tgtgtccctc cggctgcctg
    21181 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    21241 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    21301 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    21361 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    21421 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    21481 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    21541 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    21601 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    21661 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    21721 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    21781 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    21841 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    21901 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    21961 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    22021 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    22081 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    22141 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    22201 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    22261 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    22321 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    22381 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    22441 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    22501 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    22561 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    22621 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    22681 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    22741 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    22801 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    22861 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    22921 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    22981 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    23041 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    23101 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    23161 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    23221 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    23281 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    23341 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    23401 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    23461 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    23521 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    23581 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    23641 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    23701 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    23761 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    23821 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    23881 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    23941 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    24001 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    24061 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    24121 atcccattac ctaaatgaac aatcagacca atcgctgttc ctatcactaa agcaaaaata
    24181 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    24241 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    24301 ccaattagtc cgccaagcaa aacggaacct acattgatca aaactccagt tggcatcaaa
    24361 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    24421 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    24481 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    24541 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    24601 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    24661 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    24721 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    24781 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    24841 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    24901 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaaa
    24961 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    25021 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    25081 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    25141 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    25201 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    25261 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    25321 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    25381 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    25441 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    25501 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    25561 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    25621 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    25681 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    25741 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    25801 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagtc caatctctgt
    25861 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    25921 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    25981 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgca aaaaaacgat
    26041 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26101 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26161 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26221 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26281 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26341 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26401 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26461 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26521 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26581 aataaaaccg agaaaacaca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26641 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26701 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26761 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26821 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26881 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26941 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    27001 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27061 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27121 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27181 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27241 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27301 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27361 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27421 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27481 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27541 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27601 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27661 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27721 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27781 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27841 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacagatcat atgcataaaa
    27901 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27961 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    28021 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28081 ataatcctga actggcccat caatacgcag atcggatcat tgaattttct gatggtaaga
    28141 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28201 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28261 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28321 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28381 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28441 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28501 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28561 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28621 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28681 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28741 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28801 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28861 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28921 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28981 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    29041 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29101 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29161 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29221 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29281 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29341 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29401 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29461 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29521 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29581 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29641 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29701 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29761 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29821 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29881 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29941 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    30001 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30061 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30121 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30181 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactccttc
    30241 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30301 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30361 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30421 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30481 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30541 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30601 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30661 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30721 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30781 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30841 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30901 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30961 atacgatcgg tatgtacatc atgcctggcg gacgccacat tggtcacttc caaaaacaat
    31021 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31081 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31141 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31201 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31261 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31321 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31381 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31441 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31501 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31561 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31621 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31681 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31741 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31801 tgagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31861 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31921 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31981 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    32041 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32101 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32161 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32221 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32281 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32341 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32401 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32461 gttaatcacc atacaatgaa acttttggat caatttggaa ttgtaaatga gcattcaaaa
    32521 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32581 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32641 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32701 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32761 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32821 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32881 gattttagat tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga
    32941 tggtgaaaaa acattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    33001 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33061 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33121 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33181 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33241 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33301 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33361 ttcacgatac tgccttcata cggcatattg tcaaaagttg cttggacttt aactcttcca
    33421 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgtcgact
    33481 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33541 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33601 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33661 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33721 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33781 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33841 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33901 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33961 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    34021 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34081 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34141 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34201 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34261 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34321 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34381 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34441 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34501 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34561 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34621 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34681 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34741 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34801 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34861 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34921 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34981 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    35041 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35101 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35161 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35221 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35281 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35341 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35401 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35461 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35521 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35581 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35641 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacgc
    35701 cgcaagcttg gatcaagcga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35761 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35821 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35881 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35941 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    36001 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36061 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36121 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36181 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36241 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36301 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36361 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36421 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36481 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36541 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36601 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36661 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36721 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36781 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36841 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36901 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36961 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    37021 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37081 ttaaccggta gttcattggt tggtgtatga atccactctg ccaattgttc cttcccttct
    37141 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37201 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37261 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37321 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37381 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37441 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37501 ttttattgga actcacttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37561 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37621 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37681 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37741 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37801 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37861 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37921 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37981 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    38041 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38101 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38161 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38221 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38281 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38341 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38401 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38461 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38521 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38581 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38641 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38701 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38761 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38821 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38881 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38941 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    39001 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39061 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39121 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39181 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39241 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39301 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39361 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39421 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39481 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39541 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39601 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39661 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39721 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39781 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39841 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39901 ttttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39961 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    40021 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40081 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40141 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40201 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40261 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40321 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aacagcttct catattttct
    40381 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40441 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40501 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40561 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40621 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40681 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40741 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40801 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40861 ccgtttacgg cccatttttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40921 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40981 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    41041 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41101 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41161 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41221 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41281 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41341 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41401 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41461 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41521 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41581 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41641 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41701 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41761 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41821 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41881 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41941 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    42001 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42061 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42121 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagaaaa
    42181 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42241 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42301 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42361 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42421 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42481 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42541 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42601 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42661 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42721 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42781 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42841 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgagattgg gagacggtaa
    42901 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42961 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    43021 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt acctgacaga
    43081 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43141 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43201 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43261 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43321 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43381 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43441 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43501 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43561 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43621 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43681 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43741 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43801 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43861 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43921 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43981 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    44041 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44101 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44161 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44221 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44281 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44341 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44401 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44461 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44521 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44581 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44641 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44701 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44761 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44821 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44881 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44941 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    45001 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45061 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45121 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45181 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45241 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45301 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45361 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45421 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45481 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45541 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45601 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45661 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45721 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45781 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45841 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45901 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45961 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    46021 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46081 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46141 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46201 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46261 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46321 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46381 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46441 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46501 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46561 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46621 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46681 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46741 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46801 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46861 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46921 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46981 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    47041 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47101 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47161 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47221 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47281 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47341 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47401 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47461 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47521 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47581 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47641 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47701 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47761 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47821 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47881 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47941 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    48001 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48061 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48121 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48181 gttttcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48241 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48301 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48361 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48421 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48481 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48541 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48601 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48661 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48721 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48781 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48841 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48901 ttgaaagaaa agtgtcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48961 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    49021 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49081 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49141 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49201 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49261 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49321 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49381 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49441 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49501 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49561 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49621 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49681 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49741 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49801 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49861 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49921 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49981 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    50041 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50101 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50161 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50221 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50281 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50341 ttcagatagt caagatcctg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50401 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50461 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50521 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50581 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50641 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50701 cttcgccctt cgcacgatgc agttggcgca aatgtacggc tatgtcattc cagagacact
    50761 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50821 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50881 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50941 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    51001 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51061 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51121 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51181 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51241 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51301 aagacaggtg caagaatcga gaaaaagacg aatggcaagt aatgcaacgc gctaactttc
    51361 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51421 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51481 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51541 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51601 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51661 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51721 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51781 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51841 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51901 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51961 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    52021 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52081 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52141 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52201 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52261 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52321 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52381 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52441 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52501 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52561 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52621 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52681 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tatttttttt atttttcttc
    52741 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52801 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52861 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52921 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52981 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    53041 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53101 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53161 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53221 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53281 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53341 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53401 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53461 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53521 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53581 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53641 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53701 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53761 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53821 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53881 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53941 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    54001 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54061 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54121 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54181 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54241 gggattcttt gagtctattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54301 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54361 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54421 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54481 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54541 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54601 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54661 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54721 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54781 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54841 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54901 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54961 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    55021 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55081 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55141 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55201 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55261 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55321 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55381 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55441 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55501 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55561 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55621 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55681 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55741 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55801 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55861 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55921 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55981 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    56041 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56101 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56161 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56221 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56281 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56341 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56401 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56461 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56521 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56581 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56641 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56701 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56761 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56821 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56881 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56941 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    57001 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57061 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57121 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57181 cttctacttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57241 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57301 cgatagattg cagaatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57361 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57421 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57481 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57541 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57601 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57661 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57721 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57781 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57841 tttatgaaag caggaacctg ctagcatgtg taattaatta tttcttcagg ataggatagg
    57901 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57961 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    58021 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58081 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58141 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58201 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58261 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58321 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58381 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58441 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58501 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58561 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58621 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58681 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58741 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58801 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58861 tgattttgtt ttagttaatt taaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58921 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58981 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    59041 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59101 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59161 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59221 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59281 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59341 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59401 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59461 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59521 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59581 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59641 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59701 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59761 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59821 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59881 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59941 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    60001 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60061 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60121 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60181 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60241 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60301 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60361 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60421 tctgaggttt attataacta tctattgaga aagtatcaag ctagcaattc cttgtttatg
    60481 aattcattga cagaaattta taaaaagaat attatgtact aaaataataa aaaaagtttt
    60541 ataaaaaggt ataaaacctt tttattaaac tatttaatct tgtttagtat atatttttag
    60601 aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc acacatttga aaaagagctt
    60661 tggtcgtttc gaagctttaa aagatgtctc tttcaccgta cattcaggtg aagtcgttgg
    60721 atttatcgga ccaaatggtt ctggaaaatc cacgacgatt cggacacttt tagggattat
    60781 aaaaaaagac ggcggaacgg caacgatttt tgggcaagat gtatgggaag atagtttatc
    60841 tatccatgaa cgtctttctt atgttccagg cgatatctct ttgtggggaa atttgactgg
    60901 aggagcgatc atcgatctct tcatgaagct tcatggaaaa gggagtgctc aaaaaaaaga
    60961 tgaactgatt caacgtttct ctcttgatcc taagaaaaaa gcaaaaaact attcaaaagg
    61021 aaatcgccaa aaagtgggct tgattgctgc tttggctgtt gattcggatc tctatttatt
    61081 tgatgagcct acttctggtt tagatccttt gatggaaacg attttccaag aagaagttga
    61141 aaaattgaaa gaacagggca aatccatcct actttcttct catattctca gtgaagtcga
    61201 gcgactagcg gataaagtcg tgatcatacg cgaaggaaca gtcgtagaag aaggaacact
    61261 cgatgaattg cgtcatttga ctcgttcgac catcactata gaaacagaag cagagaatag
    61321 tcacttagca caattgaacg gtgtccatga ctttgtgcaa aaaggtcacc tcgctcaatt
    61381 tgctgctgat aacgaagctt tcgatgtgat tttagctgaa gcgtccaaat taggtgtgaa
    61441 aaaatttgaa tctattccac cgactcttga ggatttattc atgagacatt atgaaggatg
    61501 aggaggaagc gaaatgactg ataaattttc acgctggtcc accctttttt ctcaaaacct
    61561 tagccgtgac tggaaaaaga tcctgatatg gctagtcggt gtcactgctt tttctggtgg
    61621 ttttgtccct gcgtttgaag aattgacttc cggacaagga ttagctggat tatacgaaac
    61681 cttgcaaaac ccggcaatga ttgcaatggt cggaccaact ccagcttcga atgctgctga
    61741 gtatacttta ggagcgatgt atgcaaatga aatgttgctt ttttgcagtt tgtttgctgc
    61801 tgtgatctct gttttacacg tggtgagcca aacgagaaaa aaagaagaac ttggtctgat
    61861 tgaacttatt cgttcctttc gagtcggacg tcaagcaaac tctttagctg tcatcgctga
    61921 aagtattctg atcaatttgc ttttagcaat atcgatcagc tgtacgatga tttttttcca
    61981 agcagattcg atcactgcag aaggtgcttg gctttttggg ctatctgtag gtagtatggg
    62041 gattttgggg agtgccgttg cactgtttgc tgcacaactg atgccaagtt cagccagttc
    62101 gaatggtctg tctttagcgt ttttaggtgg actttacctt ctacgagcaa taacagatgt
    62161 agctgattcc tccctttcga tgtggaatcc tttaggctgg tcttatttga cctatccatt
    62221 tacaactaac aactggattc ctttgcttta tttgtttttg ttcagtctaa tcttgataat
    62281 cagttcattt gttttagagg aacatcgaga tttgaacacc ggctatatcc ccgaacgtga
    62341 aggaaaaccg tatgctaaac gttctctttt atccttgcgt ggtttattat tctatctaaa
    62401 taaagggatg acaatcagtt ggcttatcac ttttggtttg atgggggcgg catacggctc
    62461 tatctacggg aatatgcaac agtttttaga aagtaacgac ttaatgaaac agatgtttat
    62521 ccaatctggc gtctcaattg aaaaaaactt tacaagcacg atcatggtcg tcttgatttg
    62581 tctagctgct attttaccaa ttgctagcat caatcgttta tttacagaag aaacccacgg
    62641 acgtatgaat cagctgttcg caacaaaaat ttctaggaac caactttatt ggaccaatat
    62701 gttactagct attctatttg ctatattggc aatcctgatt agttctggtg cactagggct
    62761 ttctgctttg gcttcgatga aggatgcgaa agacatggat ctattgatgt ttcttaaagc
    62821 cgggttcaat ttcctgcctg ccgttttagt gtttatcgga attagtggct ttctattcgg
    62881 atggcttcct cgaatgggta aacttgctta tatctactta gcttactcct ttgcactcaa
    62941 ttactttggc ggaattttgg atttaccaga atggttttcc aaaacagctg tccctagctg
    63001 ggttccacgg atgccaaacg attcttttga tctttccgtg ttccttacgt tatcatgtat
    63061 cagccttgca ctgttcgtcc ttggttctat tggttatagg cgaagagact tactagaagg
    63121 ataataaaaa agcatgtaca atttcatgct agcctccaaa tctgggtcaa aaatccaaca
    63181 tttggaggtc atttttttat ttacaagtct tctaactgct ctttatcaat gatccagact
    63241 tctcgattct ttctttttac ataaccattc tcctctaata atttaaattt tctagataaa
    63301 gtttcggggg tcgtaccgat aaatgcagca agttctttca ttttcattgg caggaaaaag
    63361 tgatccttcc ctgtcgcttt agctaagtca agcagataga cagcgactcg ttcttctact
    63421 ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg ttaatttctc aacgttgatt
    63481 gctaaaagtt tcagactaag ggcgggataa tgcagtaaga gttccgagaa atctttttgt
    63541 ctcaatatgc aaatttccgt tttttctagt gcttcgccaa ataaagactc atttggaacg
    63601 cctagcaact ggctctctcc ctcatatccg cccggctcta ttactcgtaa aagctgttcc
    63661 tttccgctag cagataattg atacactttc atactgccgg aggccacgat cactaattga
    63721 ggttctccat tcggcgcaag aatctgttct cctttttcca catagtgatg tttgacaagt
    63781 gcgtgtactt tttcttgatc ttgctgtact aagtgattga acaaaggaac aaatgaaaca
    63841 cataaatgat catgactcat aatctctcct ccttctaaac agctatttct aataaagaaa
    63901 cataacttcc cttattccaa ctgataattt tcttttatca tagcataaac gtcaagcgaa
    63961 ccagatacca gcaaatagag tggatttctt tttgtccctt gctttatttt ccttcttttg
    64021 agaccttaag taattgattc ctatcaattt tttaaatatt tactttttga ataatcaaaa
    64081 acaaaccttc actcgcaagc cgagagtaag gtttgttttt cgcattgctt atttcgtgtt
    64141 gtctattgaa atttttccgc acctagcttt tgatatagtg gtgctagttc ttgtaaggtt
    64201 tcagcggtta tttgctcaac gtgttcgatt tcttgcggat agaagaatag tttacccatt
    64261 tgaaaggttc ggtctctcat cgcttttttc aactcttctt gtttctttgt atcggacaaa
    64321 tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg gttgcggcca tatgtaataa
    64381 tccttgatat cgactgtttt gctccactct ttcagtatcg caagcaactg gttatacgct
    64441 gcaatgctac catatcgttc ttcgctttta taatgttggt acatcaaata gatttggtat
    64501 tgtttttgat tcaacaaaac tgctagacag gcattctcgt cttgacgaga ttcgcttcgg
    64561 tacgcgctcc aaaaatgact gcgtaaattc cagccattcg tccagctttc gattttaggt
    64621 ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct ggagctgaat ctctttccat
    64681 ttttgccagt gttctttata ttctttcttg atttgttcaa tcttatctgt tggatggctc
    64741 tctttgagct gtttgaatgc gtagattggt cgatcaaaca actgataatc tgattttcca
    64801 agttccattg tttcgcctct ttccgttatc ttttgtattc tacctgaatt ttgcgatatg
    64861 gacaaataga ggttgtgaaa aagctgttct gcatcaagtc ataagaacag ccaaacaaaa
    64921 aactgcgatg agcatcaccg cagttttatt gaacttaagg ttgattgatc acatctaaaa
    64981 gcgttgaaaa taaaatggca ctagaagcag ctcctgggtc gatatgtccg attgcacgct
    65041 ctcctagata ggaagctcgt ccttttttag ctaccaagac tttggtgtgt tcttttgcct
    65101 ggtcaatgat ttcttttgtt aaattccctg ccttcaatgc ctctgtgact ggcagccaaa
    65161 catctaacat cgttttatcg tcagcttctg cctttcctcg gctttgaata cccgctgttc
    65221 cctgctcgat cacttcgcct aatttttctt gagaatcaat agaatcgacc tcttttgtcg
    65281 ctttcatcat gttcatgaat gcagatccgt ataacggacc agaagcccca ccgactttcg
    65341 atatcatcgc catagagaat actttgaatg tatcgctgat tgtttcagga tgatcggctt
    65401 gaaaagcttc ttcataagct gaaacccctc ttcccatgtt atttccatgg tctccatcgc
    65461 caatcggggt atctagatca ctgagatatt gtttgttctg attgattgcc tctgcaaatt
    65521 gcgtgagcca ttcttggatt tcttgaacgg ttagtttcat attatcaccc ttttatcttg
    65581 agatttttta ccatgaaatc gtctgtacat tgctttctaa agaatctttc cattgtgtct
    65641 ccgtcaagtc gatcagtgtt agagaaagac cttgcatatc tagtgaggtc ataaaattac
    65701 cgacttttcg gaaaactaca ttgacgcctc tttcttccaa cagagctaaa acatcattca
    65761 tgaacacgaa ctgttccatc aaaggcgtcc cgcccattcc gttgactagt acacctgctt
    65821 cttttggtaa attcgagtaa tcatctaaaa tcttagtaac aagttccttt gccagtactt
    65881 tagaaggctg catcttttct cgacgatagc ctggctcacc gtggatacca acaccatact
    65941 cgatctcatc ttctcctagt tcgaatcctg gtttccctac ttctggtaca gttgctgctc
    66001 gaagcgctag accgatcgtc ttagttgcct gtacgacctt ttctcccagt tctttcaact
    66061 cagctaggga agcaccgttt cttgctgcat cgcccacgat tttgtgtaca aggacagtac
    66121 ctgcaactcc gcgttttcca gcagtatacg tactattttc aacggcgata tcatcatcta
    66181 cgatcacact ttctacttca atatcgtcca tagcagctaa ttcctttgcc atatcgaagt
    66241 tcaatgcatc ccctgtataa ttcttaacga tcagcaaaac tccttttcca ctatccgctt
    66301 ccttaatagc cgtctgaatc tgatctggag ttggagaagt aaacacatca ccaagcaccg
    66361 ctgcacttaa catcccttcc cctacaaacc cggcatggga tggttcatgt ccgcttcctc
    66421 caccagaaac caaaccaact tgttccgctt tctgattctt ggctacaaca cgggaagatt
    66481 ctacacgatg aacaagttct ggatagcttt ttactaatcc ttccagcatt tcttcgacaa
    66541 tattgcttgg atcattcatg atttttttca atttattccc atcctttctt tccggcattt
    66601 tgtgcttctg ttaagatttg ctgagtatca tctgttatac cggcagtgat ggctgctgca
    66661 aaagctcctt ccactaaagg cgcatccacc agatgataac gttgctgctg ctcttcttcc
    66721 aacatgtctt ttgccagttc cgagttcaat accgcgctac cgatatccgc aaaaacaaaa
    66781 taactagctg catcgcttga ttcattgact gcttctacga ttttcatcgg gtctgatccg
    66841 atctcgccat ttattccgcc gagagaaaaa atttgcacct cttcggattt ttgcatctgc
    66901 tcaatcattt ctttaatacc atctgttact ttttgactat gagaaataag caaaatactt
    66961 tttttcacaa gaatcttcct ttcttttaca tagttgctaa cgattggttt cgctttcatt
    67021 aatgaacaat tcaagtatat aataaatttc taattttggg aagatatgtg tagtgcttct
    67081 tgaagtttgt tatactttaa ttaaaagaga ggagtgatcg aatgattaca acaacaacga
    67141 acagtgtaga gggaaaagcc gtagtagctt ataaagggat cgtttttggt gaggtcatta
    67201 ctgggatcca tgcatttaaa gacttggagg cggggttgcg aaatatcttt ggcggccgct
    67261 caaaaagcta tgaaaacgaa ctaatgggtg caagagaaga agcacttgcc gaaatggccg
    67321 aacgtgcaaa agcattagga gctgatgcga tcattggcgt aaaaatggac tatgaagtat
    67381 taggcagcga caatggcatg ctgatggtca catgtagtgg aacagctgtc tccacgagat
    67441 aagtagggga catctgtttt aataactaaa gagagatggg acatgtagtg gcactcaaga
    67501 attagatttt tcagtctaac ttcgaggtac tctaccttcc catcctctct tttttatttc
    67561 tttttgcgga tgattccgta agctgctgcg actaacacac ccgcccctgc taaggaataa
    67621 ctgacagaag ctgttttttc atttgttttt ggcaaagtct gacttgttga tttggatggc
    67681 tctgtcgttt gctgaactgt ttctgtcgtg cttccagagg tcactgattt ttctcctgcc
    67741 gaatgcaaaa tatcttttat tcgttggaaa ctagctgcta agtcagcttt ttttgcttca
    67801 aaatcattag caactgtttg tgcttcgctg atctttccgt ggattgcttg actggccgct
    67861 gtgatatccg cttgagtttg acttagcttt ccagaagctg tttgagcaac ctgatttaat
    67921 tgctgattga attcttttgc ttgttggatc ttcccgtcaa gatctgtttg gacagcttcc
    67981 gcttttgctt tcgcttcggc aagctttttc tcaagttgtg cacggtcatt tcccacttta
    68041 tttttaagat cagctaaatc ttgatcgatt gctgcttggt cttctttcaa tttgtttttt
    68101 gcagcttcta atgcttcttc gagttctttt cttttatttt ccaagtcttg caaatgatcg
    68161 atttggtcag aagaggtaga actatttatt gatgtatctt ctgaagatga aatcgtttct
    68221 cctgaattat ctgtcggtac attggaagtc atttctgttt ccgaggcacc gttttcagca
    68281 ccagaagagc ttatagttgt tgaaggttct gtttgagcag gttcagaagt ttccgcaaaa
    68341 gctgtctgtc ccaataagaa aatactggaa acagcaattg gccagattaa cggcttcatt
    68401 ctttttttca tcacttttcc catccttact actttttaac taatttctta ttataagcaa
    68461 aaatgtttgg aagaaccata aacacaacag gctgtttgtc taaaaaacgg tgcatctttt
    68521 atttttttgt acgttccggg cttttccatg tcccgcttcc ataacctaaa atcgttttga
    68581 ctgctggaat gaaagtcgtc acgatcgtta tcgcgttcac tatccagaac aaaagcatat
    68641 atagtggtgc gaaaagaagg tatttcaatt ttcttcgtcg atcatctaca atcagcgaag
    68701 ctagcaactg gaaaaatcct gcgaccattt caaagcagat gaacacaaaa ctgaatgtaa
    68761 aagtaatata aatctgttcg tagtttcctt gatatacata atgcccgatt aatccagcaa
    68821 aaagaactac ggaaatacaa aagaatattg accagacaat actcaatgtt tgatcaatga
    68881 agatgatcgt tcttccaata tgtttaaaag gatggagcat cacttttttg aaattcgtca
    68941 gccagacttc tgttccacct tttgcccaac gttttctttg tcgatatagc atcttcaacg
    69001 tcacaggtac ttccatgaaa aagataatct caggagcaaa aacagataac cagtcattta
    69061 actgatgatc ccaagcaata ctgatgtctt ctgtcgctcg atcttgtctg aatccgccca
    69121 catcgatcaa ggcttctttt cgatacatcg tatttgctcc actgtacgca taaagaccac
    69181 cgaaaacagc tgactgtgtt cgtttaatga tgcccacgat acttgagaat tcaaccgttt
    69241 gagattttgc aatcaatttg gtacgatttt ggacatccat atttgcagtg accgcagcaa
    69301 tatgtcttgc accagggcga atgaaataat tcacatacct aatcagtgca tctggctccg
    69361 gtaccgtgtc tgcatcattg cttaaaatca atttcccttt tgcaaaggca agtccgatat
    69421 taaacgcgtg ggcttttcct ttattttttt cgatccgaac cacacgaaga ttagcgtatt
    69481 ttttcatcag tctagctaat atttctggtg tttggtccgt tgaaccatcg tctgtcacta
    69541 atacctcata gttgtgataa ttcaatttcg tcatcaaata ctcgattgta tcttcgatca
    69601 caatttcttc attatgggct ggcaccatga tcgtgatgaa cggttcgaca ctcaagggaa
    69661 catctaccca ttctttttgc ttatgtttgt ataaaaacac ataacaccag acaccaataa
    69721 accatacaaa tccgcctaaa atcggataag aaacgagaaa aaatacataa tagacaaaca
    69781 agtatctaag aataaattac ctgtatgggt gattgagatc atttcagttc caccccattt
    69841 tctttataca aatctgcaat gaatgtcgta tctagatttt tctcttcgga gacagaataa
    69901 aaacggaccg tttctctctc ttcttttgtt ccaaaacgtt ccgtatacac ttcttcaagt
    69961 aattcttgtc tttttttcaa tttttcatct tcatattcaa aagatttatt tacttttttg
    70021 tatagatttc gattgttcca cctagtcaat acaaatgagg taatcaggaa actgaaaaaa
    70081 atcaatacaa aaaagccgat taagaaatac agcattttct tttctgataa aaaagcgtac
    70141 aaggaaaaat ccttggctct ttctggtctc gttaatggga aaaagaccca tataaaagga
    70201 agaacaacga tcatccatcc taaaagagcc actaacgttt gccaaatctt taaccagtaa
    70261 ttaccttttg aaaaatactt atctttcacc aaaattgatg caacttcttc tcttgttact
    70321 tttttcatat cctcacctca cgtttttttg ttacgacgta ggagtttacc atacatttca
    70381 tttcttttca cataaagtgt tcctaaattt ttcctaagaa aaagaacaaa acatttctca
    70441 tcagcaaatg aaaaatattt tgttcttttt atcagttgtt ctttttattt tttatctctt
    70501 aattatgttg ctctttccaa tacgacaatg cttttcattc ctctagatat cgccgtgtac
    70561 atttgtctgc gtcttctttg ctcatctcca tcgttttcaa atggatataa gattacatga
    70621 tcaaattcca gtccttttgc tagactcaaa ggaatgattt ggcagttttc taatccatat
    70681 gaattttttt taatcgattc ttgtagcgtt tgggtttcct cttctgtttg ggtgataact
    70741 gccgcttttt ctccttttag agaaaccaaa atctcagcta gtattttcaa caattcttca
    70801 gaggatcgaa aagagcggta ttcgggttct tttccttgcg gtcggatcga tacgatgtcg
    70861 atcttctctt gatccacagc ataggttttg aaaagctctg tgattgctcc gctagaacgg
    70921 taactatttc taagatcata ccgatggatc ggcagatggt agtcgtcgaa acaacgcata
    70981 atatcagcaa aagtagtgga agaattaaag attgcctgat tctcgtcacc tactaaagta
    71041 aagtgagttt ttggaaataa ttcgatcaat agacctaatt gtgccagggt ataatcttgt
    71101 acttcatcaa tcaatagata gcgaaatgct ggtacattta ccttttcaac aagtaaatga
    71161 cgaatagcta atataatgac tgcttcatca acagtacgtg tgggttgcca agcataagct
    71221 gagccatatc gtttttcata aattctttca aacaactgat gctcatccac ccatgccatt
    71281 tcttcgattt gtctggttat ttttcgatat tttttttgta aaagtttcct tgcataagct
    71341 acaatacttt gttccgaatc atcagaaatc aatttaccga ataattccaa ctgcctatct
    71401 tctgtcaacg agagtacctg attctgcaca gcaggacttt ttgcttgttt caaaagatga
    71461 ttcttccatt cgctcatcag tgcctttttc gttgcttgaa gcctatcgat caatttagga
    71521 tgagcaggaa ctttctcata tattttttcg atcgtttttt tagaaaggac tgtttttcca
    71581 tttttagtaa gtccacggat aaaattcgga tgatctaaaa acaaaggatc tgattgtttt
    71641 agattatcga taaacttttt gctacgcaaa cgttctgtct gctccgatac attttctccc
    71701 gtgatacgtt tgaaataagc ctcttcacct tctatttcct ctgctgaaag ctgtgaaacc
    71761 agctgtatga tggttagatt cagtggattt ctttcaccta atgatggtag gacattcgaa
    71821 atatagtcga taaaccgatg attaggtgat agaatcagca agtcatcgga tgtcatcgta
    71881 tctcgatata aatagagcag atacgcaata cgctgcatga tagcagaggt tttaccactt
    71941 cctgctacgc catttaccaa gatattttga cttttcacat cccgaatgat tgtgttttgt
    72001 tccgattgga tcgtagcagt gatagccttc atttcattgg tctcatcttg gcctaagact
    72061 tctaataata cgtcatcttg aatagctaca gacgtatcaa aaaacttgac caaacgatct
    72121 ctttccaaaa gaaattgtcg tctttcttta ataaccgtat cgattttatt ttgacgaacc
    72181 gtataagaag atggaccaat catgttattg taaaaaagtt cggcaattgg tgaacgccaa
    72241 tcataaacaa gattttcatc tgcatcatct gtgaaatttg ctgtaccgat ataaaaggat
    72301 tcttttggct cattatccag aaagtctaca acgacttttc caaagtaggg acttttcaat
    72361 aaacgttcga tctttgctag ctgattttct gcgtttttct gtttgatatt catctgatcg
    72421 atttctctat ttttcatttc aatcatcgaa aaagtatcca ggttatctaa gtaactgtca
    72481 aaattcaatc gaagctcgcc agccatttct ttcaacgact ggtttccttc tatttttgcc
    72541 gtatccatcg attcccgcag ttcgtttttt ttatcaatta gcttttgata cacgttcttc
    72601 agatatgctt cttctaactg tttttgtgtg ttgaccatac acattctcct ctttttttaa
    72661 agtggtggaa ccgattagtt tacctctttt aagagtcgaa tgcaagcttt ttaacgagta
    72721 tcatttgtta ggatatatca gttcttcagg tcggacagtt tgatataaag aaaatgcacc
    72781 atcaaggttt tttacgtggt agccagactg cttcaagatt ctttccgcta gatagctacg
    72841 taatccgcta tgacagctga cgatatattc ttgttggctg tctaattcat ttaatcggtc
    72901 ccgaagttca ttcaaaggga tatgtttggc ttccttgaat ttgccctttt ccaattcttc
    72961 tgctgttcgc acatctaata aaatttttcc ttttgctagt tctgtcggca attcatgcca
    73021 ttgtacggtt tcactaagcc cttctgcaat gttcattgct gcatatccta gcatgtttac
    73081 tggatctttg gctgagccaa atggcggtgc atacgtcaat tctaattccg gtaaatcgaa
    73141 gatcgtcaaa tgccctttga ttgctgttgc taaaatatcg atccgtttat ccacaccttt
    73201 tgccccaaca ccttgtgcac cataaatctc gccagtctct ggatggaaga tcaacttcag
    73261 taaaatatct gttgcttctg gataatagct tgcatgatct tttcctgata catgaatgac
    73321 ttcaaaagac agttcgtttt ctttggctgt tctttcactt aaaccggtgg atgcggcagt
    73381 cattccaaac gttcgtataa ttgctgttcc aataccgcct tgatttcttc gagaaattcc
    73441 ggaaatcgtg tccgcaactt gtctaccttg acgattggct ggtgaagcaa gagaaataag
    73501 tgcatctttt ccagtgattt gctgttttac aacgatagca tcccccaccg cataaatatc
    73561 aggaatattc gtttgataac gttcgtctac taggataccc ccgcgtatgc ctaattcgat
    73621 tccagcggtc actgctagct ggttatcagg acgaacacca atggataaaa taactaaatc
    73681 agcagggatg gtacgcccat catccagtcg caatcgtttc ccgtggtctt caaatccagc
    73741 gacagcatga gaagtaatca cttctactcc ttgatgagac aattcttttt ctataaaagc
    73801 tgccatttct tcatccagtg ttggaagaat atgtggtgct tgttcgatca ccatgacttc
    73861 taaacctctt ctttttaggt tttctgccat ttccaatccg atgaatcctg caccaacgat
    73921 cacggcacgc tttggctgtt tttctaatgc atgcatcact ttatcgatat ctggtacatt
    73981 tctaatagaa aaaacattgt cggcttctgt tatccctgga atcgatggaa caactggttt
    74041 tgcaccagga gataaaacca atgcatcgta ctgttcaatg tgtttttgac cctcatgtac
    74101 gacttctacc gttttgtttt cgggaaagat ggctgtcact tcatgatttg gacgcacatc
    74161 taaacaaaaa cgagcggata atgattctgg cgtttggaca agaagatttt cccgttcact
    74221 gatttctccc gaaagataat aaggcaagcc gcaatttgca aaagaaacat acggtccttt
    74281 ttcaaaaaca acgatttctg catcttccat taatcggcga agccgtgtag cagcagacat
    74341 tccacctgct acacctccga caataacgat tttcatctta tttccctcct actgttcgat
    74401 caaaccattg attcatgcca ccacgtacat tcactacatc ataaccagat ttctttaatt
    74461 ctttagctgc ttgtttactc ctcatccctg attgacaaat cacatagacg gtcttttctt
    74521 tgtctccatc atatctgttt atactttgta atggaacatt tttagctcct tttatatgcc
    74581 cgccccggta ttctgcaggt gttctaacat caagtaacgc tggattttcc aaaagctttt
    74641 cctttaattc tgggactgtt atcgatttta ttgattgaaa tagcaaattc attttctgca
    74701 cctcatttac atttttataa atacctcatg gggtatatta aacaggagct ttattaatat
    74761 gtcaaccaga atgactcttc atttttttca ctacttttta tatttgcata aaataccgat
    74821 aaggggtatt atctaattat aaaaatagag gagaatgtgt tatggagatt gataataaaa
    74881 atatcctgaa ccgtttgaaa cgtacagagg gacaaattcg tggtatccaa aaaatgatcg
    74941 aagacgaaaa agaatgtatg gatgtcatca ctcaattaag cgctgttcgt tctagtattg
    75001 atcgcgtaat gggcatgatc gttgcagata atctgaaaaa ttgctttgag aaacctgata
    75061 gtaatccaga agaacaagcg gctaaactag aacaagcgat caagatgatt attaaaaagt
    75121 aaaggaatga tttcaatgaa agtcgttttc catattgatg aattagaaaa atggtcagaa
    75181 acagggaaaa acgtaaaaaa cctgataaaa gcttctccag aaacgacgat tgtcgtatct
    75241 gtaaatggca tcgcgattac cggttatcta gactctgcca atgcagaatt cttagatttg
    75301 caaggagtcg tttttcacgc ttgcgccaat gctatgcgtg ctaatcatat ttcagaaagt
    75361 tctttacctg aacaagtaat cgttgtacca gcaggtgtcc ttgatttagt tgaactgcaa
    75421 tcccaaggtt atgcttatat caagccttag tcagtagagg ttgcgacaga agtgatcagc
    75481 ttcaagaaat ttcggcttat ttttcgaaga agctacttct ggaaaaccgt gaatcaaagg
    75541 ttgtgaaaac gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat
    75601 atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa
    75661 gaggttgtga aatcagcggt ttgacctaag tattagagaa aaaaacggga aaatagctcc
    75721 tcatattttg ccgttttttg agctaatacc gaaggtcagc tgattgaaca ccgtttaagg
    75781 aagagattgt gaaaaagtcg cttagtttcg agtattaaga aaaattttgt gaaaatggat
    75841 ttctacattt ttgcataaat ttggcttaat accgagaaac tggcttttga ataccgttta
    75901 aggaagaggt tgtaaaatca gcggtttgac ctgagtatta gagaaacaaa cggaaaaata
    75961 gctgctcata ttttgccgtt ttttgagcta ataccgaagg tcagctgatt gaacaccgtt
    76021 taccaagagg ttgtgagcct accatttaac tccgaatcac aagaaataat ttcaaaaaca
    76081 acttctcctg tttttggttt ggttggctta ttatcgagaa gtagctactg ggagtctatt
    76141 tatcaaaaaa aggaatgcga cataggtcac accccttttt tattttcttt gaaacatctt
    76201 tcggtactcg acagcataca agacaaaaga ttgtaaaaga ccgtaagtga tgacggcaaa
    76261 aatgactctc acccacagcg ttggtaattc tgggaaaagc agcaaaagga tcatgccaac
    76321 atagatgaca gcagtcgcta ctttgccata ccacttcgct ccacttaatt tgatattttt
    76381 agatagcccg attaatccag ctacaaacat aaagccttct tttatcaaga atagaccaaa
    76441 caacagccaa agccacggtc gataccatgc catagacaaa atcagaaaaa gttgtgtcaa
    76501 tttatctgca aacggatcaa gcaccttccc taagtctgtt acctgattat atctccgagc
    76561 aatgaccccg tctagataat cagacaaacc tgagacaaca atgaccgctc ctgctatata
    76621 aaatgactgt ctcagcacaa aataaagata aatcggcagc aaaaaaatac ggaatatcga
    76681 taaaagattc ggaatcgttt gtatttcttt acgccagtct gtgcttaatt tcattttatc
    76741 gccctacctc ctcagcactc ttttcttttt attttagctt acacggggaa taaaaccaat
    76801 gatttcactg tttttatttt tctttacagc cactgatatt atgagctaat ttgagataat
    76861 cacttgctgt tttcatcgtt tactcttctt tcttcctgtc gtttgcgcat ttcttttact
    76921 agtactttat gagctttttt tgatcctcgc tctaggtcac gctgccgttt acgtgtttta
    76981 taatcgataa acagactgtc taaatcgtaa cctgtcgggt acagatcttc tgctgaacgc
    77041 accagacgaa cacgctgacg aggcacgtgg attatttttt tgtctaaata tacatcaatc
    77101 gtatcttcac ctatatcttc ataaactaac gcttcttttt ggtattcatt gacaaatatc
    77161 cgatcacctt ttgcaaagag ctggcgttct tttttattct ccttcctatt tgtaactgct
    77221 gtcttaggaa gaaattcttt tttctgtgtc cgataggctt tttccgccaa gtaacgctct
    77281 gcttgatgga tcaatttcat cgacatatcc attttatgag caatccacaa agcttggctt
    77341 tctcctgttt ctccaacccg caactgatat ttaggacgaa gtgcttcccg atcgaaagcc
    77401 atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc tccataatgg
    77461 gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc gatcgctagt
    77521 gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac caacgtattc
    77581 ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa tgtactcaac
    77641 gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc
    77701 tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt caataaaccg
    77761 actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat caagcctcga
    77821 tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa tggatgtctg
    77881 ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc attgatttct
    77941 cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc gatcaataaa
    78001 tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac ttgatattct
    78061 tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga aatcacagtt
    78121 ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc atctatttta
    78181 tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac aatcattgct
    78241 tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt tgactggatt
    78301 tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga cgcgtcgtca
    78361 ctgactttat tattttttat tttctggtca atcaactctt ctacatttat caaatcagga
    78421 agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc aaaaaacttt
    78481 gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt tggctgcaga
    78541 atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga
    78601 acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg acgatccaag
    78661 acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct atagtttcca
    78721 attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact aatcatttcg
    78781 ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat aagggcctag
    78841 agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt attagaatac
    78901 aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa ctttgtttat
    78961 tggctattcg acttactgat tagataccag ctacactcac aaaacatacc ccattcttct
    79021 aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc aaactttagt
    79081 atgttttata gatttacacc attaatgaat agacacctac cgcgataatt gctccgatca
    79141 ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta ttagctattg
    79201 gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt gcatatcctg
    79261 ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg aatacgttcg
    79321 ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat acaaatgtac
    79381 caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct gttgcaaacg
    79441 ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa gaaagccaaa
    79501 caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga acgacaagat
    79561 tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc aaatgagctg
    79621 ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa ccaaatgcga
    79681 tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc gcacacacac
    79741 catctccaag caaaactagg atcagtgtcc caataaactc tcccatgatt tgagtcatat
    79801 cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca atcaatgctt
    79861 ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat ccagcaattg
    79921 catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac agatgatttg
    79981 tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa ccataacgta
    80041 accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt gctaattcaa
    80101 agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct tcttcctcta
    80161 aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat ttagttggat
    80221 cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc tctattccgt
    80281 attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat
    80341 cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct aaagaactac
    80401 ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg ttcaacacgt
    80461 cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta tcgacaactt
    80521 tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca gaaccagaat
    80581 taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc aatgtgatat
    80641 ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct tcttgtgtga
    80701 ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt
    80761 tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg aagtatgtgg
    80821 gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta gttggacgca
    80881 ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc caaggaccac
    80941 tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc aggtcacgag
    81001 ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc attttactta
    81061 ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa cgggcatcat
    81121 tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc aaaaacggtt
    81181 ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt cctgtcacat
    81241 ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg aaggttgttg
    81301 ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa
    81361 tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca tcaaatgtct
    81421 tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa
    81481 aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg actgccacac
    81541 cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc tgcatttttt
    81601 gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta ttcaaatgct
    81661 tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc
    81721 atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc tgtatctttc
    81781 caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt ttccaagttt
    81841 tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag atattcattg
    81901 tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc atcttgcatc
    81961 gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt aatgatatct
    82021 tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg atcccaataa
    82081 ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct tgtagagcgt
    82141 ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc tctcagccat
    82201 tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac ctttccattt
    82261 atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt ttcaccggta
    82321 ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc
    82381 atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt
    82441 ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt gacttttggt
    82501 aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc tagctgatgg
    82561 atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc ttgtccatcg
    82621 gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc tttttcagct
    82681 cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc tgagaaatag
    82741 gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc attttttaag
    82801 ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata gattggacgt
    82861 cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc aatcccagca
    82921 atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga ctgtacagaa
    82981 ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata ttgcgtgaac
    83041 tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta
    83101 gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat tccctccatt
    83161 atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa aagaaactgt
    83221 caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct attttttaat
    83281 caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat gtcgtaaggt
    83341 gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca aaatgtgatc
    83401 accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc gattcccctc
    83461 agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact ttctaaatcg
    83521 attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac attggcatac
    83581 tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa ttgctgcacc
    83641 gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat tttctgctgc
    83701 tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa aaaccattca
    83761 ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt caaacgacga
    83821 ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc ggttggtaac
    83881 tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat cgaaaaagaa
    83941 acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc tgttcgacta
    84001 agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg atagagtaat
    84061 ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt tttcgtaatc
    84121 ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc taatacacgg
    84181 tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga
    84241 tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag ctcttctccg
    84301 tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt caattctttg
    84361 atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg aacgatggat
    84421 acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc ctgcaataaa
    84481 aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc ctgatactca
    84541 tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat atcttccaaa
    84601 tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt gatagaggct
    84661 gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc ttttctttct
    84721 aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca tatctggcac
    84781 acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg tccatccttc
    84841 ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc tttggagctg
    84901 tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt tgctttgatt
    84961 tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct
    85021 ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat tgccgactgg
    85081 acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga catttcgtgc
    85141 tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg gacgaaaagt
    85201 tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca agtagtcatc
    85261 tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct gattgtcggg
    85321 gcgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt ccgtctctac
    85381 aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc
    85441 ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat aacgaaaaag
    85501 aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca acccttcagc
    85561 tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat tcatgatctt
    85621 ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg ttgattctgc
    85681 agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat caaaatccaa
    85741 tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg tcttttctgt
    85801 gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag aaagttgttt
    85861 ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc atcctaactt
    85921 cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa gataaatagc
    85981 cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca ttcctctttc
    86041 ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat aactcgcttt
    86101 caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac cgtgttctag
    86161 gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc tgaagagatg
    86221 tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa
    86281 taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat agttagaata
    86341 gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct aattgatgag
    86401 cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag
    86461 ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct aagaatggta
    86521 cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc actgtacgtc
    86581 cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca acaccgtgac
    86641 aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc ccaagatcta
    86701 ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc gctgcataat
    86761 catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt
    86821 catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc ggttctaaag
    86881 gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt actaatacat
    86941 cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc ttttcgtact
    87001 tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc tataaaaatt
    87061 gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat aaagctcttt
    87121 atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca ataatttctc
    87181 tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg catatttcaa
    87241 tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag ggatctttcc
    87301 ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat cagaacgagc
    87361 tattcgatca tacatattta atgaaacatg cagataatct agttttgact cacgtaatcg
    87421 ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt cttctggtga
    87481 gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg agaaaaccgt
    87541 tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc attgatcctc
    87601 tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa
    87661 ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa atgcttggat
    87721 tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag gacgttttgc
    87781 tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac ttgtcattcg
    87841 tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg ctttcgctag
    87901 ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg gccagccttt
    87961 tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt tagaacgttg
    88021 tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga gttgtgtcgt
    88081 cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag taattgattg
    88141 atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta tcgagaatca
    88201 tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa aaattttatt
    88261 ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta cttgcttcat
    88321 ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg acttccttag
    88381 caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt
    88441 tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat ggggcggaat
    88501 agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat atcaataaaa
    88561 taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct aatttattca
    88621 acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct ttttcaattg
    88681 gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt tattttttgt
    88741 caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat
    88801 cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt gaatcagagg
    88861 ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac
    88921 atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg tttatatcag
    88981 aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
    89041 catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac cgtttattga
    89101 gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa aaacagcttc
    89161 tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat actgtttatt
    89221 cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt ttattatagg
    89281 atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt ctttaaccgt
    89341 ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata acgttttgac
    89401 atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata accaaataaa
    89461 tctactccat ctttgatagc ttccttcatc tgctcaatat gctgtttcaa atagtcaatg
    89521 cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc gagtccgttt
    89581 tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc aattctcaaa
    89641 cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt tttaattcct
    89701 ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact gactgcacta
    89761 gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat ttcttcatca
    89821 cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt atacgctgga
    89881 taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg ctcaattgtt
    89941 ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct gatcatacaa
    90001 ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc acttgctata
    90061 aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt ttcttcaatt
    90121 actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa agtcatccaa
    90181 tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac gtaaaactga
    90241 ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg catttcataa
    90301 tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa cacttgatca
    90361 taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa aattcttgtc
    90421 catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg gatgtcttct
    90481 ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt gctgttggga
    90541 tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt ccatttttct
    90601 ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt ttcatctaca
    90661 tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa tccttctgga
    90721 aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta tttttcttat
    90781 gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt gtggtcggat
    90841 catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg cctttacgta
    90901 ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc actgtatcaa
    90961 taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt gcatgtttcg
    91021 ttggaaaaac tgttgaaact tccccgttta caggactgta gatttttccg tccgttggaa
    91081 tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt acttcagata
    91141 aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag gattcttctt
    91201 cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc atcgctaaga
    91261 aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa gacaaaatac
    91321 ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca ccaaatatag
    91381 ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca ccataaacag
    91441 caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa ctttttactt
    91501 tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca gctacggcag
    91561 tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg gtcattactg
    91621 gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg ccgataacag
    91681 caccagataa aacagggcta aagttataaa cagctaaata tccagctgca atcagctgac
    91741 ttgcataatc tgcaattgga cctacaacta ataaagtaat tgggaccatc acgatgatac
    91801 aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga atcttaggct
    91861 caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt gtataccttg
    91921 ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat gcactgacaa
    91981 tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct gtattgaact
    92041 tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca tcagctgctg
    92101 cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc gtacataagg
    92161 cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag
    92221 cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct ctctttccac
    92281 tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact tcatttactg
    92341 catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc acaccttcta
    92401 gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag cgtaaccttg
    92461 ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg acaatatctt
    92521 tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc gtagcaatcc
    92581 ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta gtacccctta
    92641 taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc aaatcactca
    92701 ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca aaaaaaacac
    92761 gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct gtgatttttg
    92821 tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac ggaagcataa
    92881 ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta tggattttta
    92941 tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt tcttcaacta
    93001 tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca tctagaacac
    93061 taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta gtcaaaattg
    93121 catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt tctatccggc
    93181 attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta ttcaaaatac
    93241 taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga ttcaacgctt
    93301 cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat tgaaaatgta
    93361 ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg atcttttgta
    93421 caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt ttgatatggg
    93481 ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa aaacgaataa
    93541 tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca tcattaatca
    93601 tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga ttggaaaaag
    93661 aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga ataaataatg
    93721 tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa acgagatacc
    93781 gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata agaacctctt
    93841 ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt tgttgaggca
    93901 accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt ctttttgaat
    93961 tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct aatttaggat
    94021 tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt tttgaaagaa
    94081 agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat aagtacgtca
    94141 caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc acctcttgta
    94201 tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa cgtttatttg
    94261 actggataat gattttcttc tcgtagatag caagcatatt tttgatttca gtttggatcg
    94321 ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa tgattcttat
    94381 ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc ttacacctcc
    94441 tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt gcagaggttt
    94501 aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg gataatttgt
    94561 ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc atcattttgt
    94621 ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc cgatacgata
    94681 tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat tgcctcagca
    94741 atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat tcgattcaat
    94801 tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg taattcataa
    94861 cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg ccaactccaa
    94921 ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc gccagtatct
    94981 acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa tgcaatataa
    95041 tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg tgcacagtct
    95101 tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat gacatcggag
    95161 aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg atcacagtca
    95221 tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc atttggatcg
    95281 attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg acaggcttga
    95341 taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc agggaatgta
    95401 accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt aaaggtcatc
    95461 caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag
    95521 tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa gggggtttca
    95581 taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt gattagtctg
    95641 tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt
    95701 gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa acgaatatcc
    95761 tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag tgattcgtct
    95821 aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg ctggttcatg
    95881 atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca tgcaccttca
    95941 cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat ttcctgatcc
    96001 ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg attcatcaaa
    96061 acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct actcttcatt
    96121 tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca ctcgttaacc
    96181 atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc gtaaatcaac
    96241 tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta aagcttcacg
    96301 tggaacagtg aacgtctgct tttcactgga catattacaa ataacgagcc attgctggtc
    96361 ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta attcatagtc
    96421 tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt acatataaaa
    96481 aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat ttggattgac
    96541 tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc attgcatagg
    96601 cgtcctagca ttgtctcttc ctttcgcatt gattgattcc aagatttcct cttttgtaaa
    96661 tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg cttccgcgat
    96721 atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat aaggggtgcc
    96781 tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat acacatcgtc
    96841 atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata aactattcca
    96901 tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt cagagatact
    96961 caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa
    97021 ttggaagacc atggataatt catttctttt aggattggaa taaagtttag cgatttcggt
    97081 agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag ttgcttgatt
    97141 catttcctga agatactcat gcagtttagg tccattactt gtgatttctt tatccggttc
    97201 tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat cgatccagaa
    97261 attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca aatcaggttg
    97321 acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact gccaagcaga
    97381 accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat cacgccaaat
    97441 ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc acggatgttc
    97501 atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt tctttgcttc
    97561 ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg cttgataatt
    97621 actgatatca tacccattgt catcattcgg agactcgtat actggagaaa gccaaattgc
    97681 atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca aatctccaat
    97741 accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa ctgaatggtg
    97801 ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt tttgcttaat
    97861 catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta ttgcaccttg
    97921 ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac tgttttttga
    97981 aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa acggtgttca
    98041 ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat atgaggaact attttttcgt
    98101 ttttttctct aatactcagg ccagaacact gatttcacaa cctctttata aacggtgttc
    98161 actcagctgt ccctcggtat tagctcaaaa aacggcaaaa tataagaaac tattttttcg
    98221 tttttttctc taatactcag gccagaacgc tgatttcaca acctctttat ttcacattca
    98281 atatccacat gaatcccaca aatacaaaga acagaccata catgattgga gatacttctt
    98341 ttcctctttt agctgctaac atggtaattg gatagaagat aaaacctaat gcaatcccat
    98401 ctgaaatact ataagtcaaa ggcatgccga tcaagatcaa gaatgaaggg atcgcaatct
    98461 ctaagtttcc ccattcgatt tgtcgcaaag attgtgccat caatactcct accataatca
    98521 atgctggtgc agttacttgt gaagtaacaa ctgatagtaa tggagaaaag aacaagccta
    98581 acaagaagaa aatccctgta gtaatagctg tgatacctga acgtcctcca acggcgatac
    98641 cagctgatga ttctacaaat gcaccaacag gagaagtacc aaataaggaa cccgcaagca
    98701 tagctgttga atcagaagct aaagctttcc ctacacgagg catcttgttg tctttcatga
    98761 aacctgcttg gtttgctaat ccaactaaag ttcctgctgt atcaaagaaa gtcacaagca
    98821 agaatgtcag cactacgacc cacatttgaa gagaattgat gtcccctaca tgttttaatg
    98881 caactaaaaa tgttggtttc aaacttggtg ctgcggcaac aatttcagaa ggcattggaa
    98941 tcaaacctgt gaccagacct aaaatcgttg tagctgctat cccgatgaag atgccacctg
    99001 gtacacgccg aacaagcaag atagcagtta cgactaatcc aaaaatcgtc aaccaagtag
    99061 aaccaacgtt gagtgatcct aatcctacta atgtagattc attcgaaacg atgatcccac
    99121 cttcgctcag accaaggaaa gcgataaata gaccgatacc gccagaaatc gcatatttca
    99181 aatctgctgg gatcgcatca atgatcattt cccgcagctt gaagattgtg atcaaaataa
    99241 aaatcaaaga agcaacgaat acgccggcta atgctgtttc ccatggaatc cccattccta
    99301 aacaaacaga ataagcaaaa aaagcattga tcccaagagc aggtgccgtt ccaattggat
    99361 aacgggctaa tacccccatt aaaacacagc cgatcgcact ggctaatgca gtcgcagtaa
    99421 acactgctcc ttcatccatt ccagaagcac ctaatacagt tggatttaca aataagatat
    99481 aagccattga gataaatgtt gtaaatcctg ccagtacctc acgttttacc gtggtgttca
    99541 atttttctat ttcgaagtag gaaagtattt tttccttcat cttccatccc cctaaatccg
    99601 aacattataa ataaagtccg ttaaaatttc gatttattaa taaaacaacg tgttttatca
    99661 tatactaaaa caaaacataa gtaaacaaaa aattcggatt ttaagcaaaa tccgaacgtt
    99721 ccatctgatt aaatcccttt atctcaaagt tcatgaacat tcactttgga ttacaagaat
    99781 ttctttattg tgttaataaa gtttcttcat tctagtctca aaaatcaata gtgtgatttg
    99841 attttaccat ttcgtttctg ttcttctttg atgaattctt ttgtatcgac aggattcaga
    99901 ttatttagtc cgccaaaacc tgtatcgacc atttttcccc agccttttgt cttctttttt
    99961 cctgatttga gattctttgc tcgaagcttc tcactatgtt ttgctcgccg atacttcaaa
   100021 taaccttcta atcttcccat tagatccact gtttttcctc ctttttattc aatcagacga
   100081 ctcagctttt cctataacaa aataattgcc ttcagggtca gaaaaattga aagtcaagat
   100141 gccattgtta tctgtgattt ctcctggttc ttccatgttc tcgtagatag tttcaaattc
   100201 atccgtaaaa aatactaaag aaggcggtgg tcctaatacc tctggtgaaa acttctgcac
   100261 aaatactttt gggaatacgc ctaattctaa ttcttttgag atgctgagta caatactctt
   100321 aaattcttct ggcaattcga ttgtttcaga aatttctgca ccaatttttt ctacaaagaa
   100381 tcggcaaatc atctctacgt cattgacata aagcatcaca cgtgtttttt gtatcatcat
   100441 ttgacccttc tttctatttg attttctttc ctgtctataa actgattcca ttatatcacg
   100501 aaagcgcaaa caccttgtgt ttgatactat tcaagaaaaa taaagcaata gtttctagga
   100561 ttgctttcct ccacaaactt tttttacaaa aaaaaaaaac ggcaatgaat gccgctcttc
   100621 ttatttcgat tcttttataa ttctgcttct gattcatctt tggttagtgt gtgtctaaca
   100681 ctagttacgc caaatgtttg atcgattttt tccttcaacg cttcgattgc ctgtgcgcca
   100741 cgtgccgctt gttcagcatt gatggattca atgaccagca ccgctgcttg tgtttcttcc
   100801 gtcagcattg ttctttgggc ttcacgccag ttgagacagc gacagatcgc tccttgttca
   100861 tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg aatctgcacc taaaggcagg
   100921 aatgattcgc caccttgggc ttttcctaaa tgcagatttc ctgcgatctt ttcgatatct
   100981 tctccgccgc aaggtacgcc aaaagacaag gaaatactat tatagatatc tacaagcgga
   101041 ttgattggga aaaattcccg ttcttgattt gcacgtttca gtaaagcttc aatagaagaa
   101101 cgtgcacctt ttttagtctt gaatgtggta aatgcctgac gccactgagc aatgacttcg
   101161 ttttggctga atgtttcttc tgtaaggtaa tttcttgctt cttttgctgc ctcattcaat
   101221 aattgatgat acgtttcttc actttctttt gttacgtggt tatcgatacc atttacggat
   101281 aagatttcga tggttgcttc aggaaatact tcccagaaat tggtatccac tattactttt
   101341 ttcatctgtt cacacctctt gattagaata attgcttgat gaatagatag tctgtttggg
   101401 gatcatctcc catataaaaa acatgttcgc tgtatttgat aaatcctttt ttacgataaa
   101461 aagcgagcgc aacatcgttg tgttcccaaa caccgagcca aatggctgtt ttttgctttt
   101521 cttttgcttt ttcaatagct gtatctagta aaaatgtacc gataccttga tgtttaaatt
   101581 tttttcgtac atagattcgc tcgatttcta aaaaatgatc agccatttcc tcagattgac
   101641 tcgttcctgt attcagtttc aggtaacccg caggctcttg atcgatactc actaggaaaa
   101701 aatcggtttc tttatctttt agctcttctg taagtttttc tagtgcataa gcttgtgtca
   101761 agtaatcagc taaatgctcc ggtgaattat cttttccaaa tgtatctgta aatgtttcga
   101821 tactaatttg ttgtagtaag tctacatctg aaggcgtcat ttttcgaatt tcgtatttat
   101881 tcacggcttc ttcctttcaa tagatccttt tattgccttt tttgacaaaa ttccaatctt
   101941 cgctaacatt cttgcatact ttatttaaaa gatattccaa ttgttttgct tcttcgtcag
   102001 aaagaccatt taatgcgact gcattggagt agttgttttc tcgaatgatg aaaggataga
   102061 tttctgctcc ttttttagtc ggatagagtt tcttgatttt tttattttct ttatcttcca
   102121 aacgttcgat catcccattg gattctaatt tcttgattgc tcgggcagtc gttgtacgat
   102181 ctactttgat catttctgct agtttttctt ggatgattcc aggattttcg caaatacgta
   102241 ctaaataaag atactgtcct cttgttaaat cgacttcttt gaattcgata ttgctaatag
   102301 aatctagcgc tcgggcaatc attccgatgt ctcgaaggat ttcagccatt tcatcccacc
   102361 tttattttgt ttgtaagact agtttacctc tttttgttgc aaatgcaact attttcaata
   102421 aaaaaatcaa gttctttttt aataactagt aaagtatatt cttttcttct tttacgaaaa
   102481 agctcaatca caatagatct ggtttttgtt ttctaaaata agtaactgtt tcttcgttgt
   102541 tcgcttttct gatagctttg gcaactcttc tatactaaaa aatcccatgt tggaagtttc
   102601 attattctca ataaacttgg cctcttcacc gtggatcgca caagcaaaaa caagtttata
   102661 atactgaaaa agctgtggaa tatctttttg tttattcgta tcaaaaactg ctcgcaactg
   102721 agtagtttct actgttaatc ctgtttcttc atagacttct ttacgcacat tttcttcagg
   102781 agacaatcct atttcagcaa atccaccagg aagtgaccat tctttcgtcc gtttatcttc
   102841 gattagtagg accttgccat cttttttgat ataggcacga acatctactt ttggtgttgg
   102901 atgtccttct ccaatcgttg tgagcgcttt tatttcttcg aaagtttctt ttcctatttc
   102961 actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa cgctctttat caaatacatc
   103021 ttttccataa tatagaccag cttgagaaag tgcgagtagt cttttgtatt gagccaactt
   103081 cttgttcatt ctcttccctt cttttttagc aaatgtatga ctatgtaatc aagtatagaa
   103141 gactggttta attacggcaa ataaatataa tgaaatctgc aataaataaa agaatgttaa
   103201 aaaactcgag aacaaaagac attcgacttc tctggggctg tgaataaatg gcgttcaaaa
   103261 gcttgcatct gcggtaataa gccccaccaa acgaaaaata tgacgagcta ttcttgtctg
   103321 gctgttctta ttaattgagg caggacagct ttttcacaac ctccccacta aacggtgttc
   103381 tcctaccaac tcctcggtca caagtcacaa tattcatcaa acatgagaag ctgttttttt
   103441 gaaattgctc cttgtgactc ggagctgaac gacaggctca cgacctctcg gtaaacagcg
   103501 ttcaaaagcc aatttttcga tattaagcca tttctttgtt acttattgtc attgatttct
   103561 ttttctaatt tatccaatag tggagtgtaa tccacttgag tatagtctgt tacatcaaat
   103621 tcatatagtg ttcctaactg gaatttgtca tattttcggt cggcgatgat ctttttgaat
   103681 gcttctttcg taatatgatt ggtccctttc gttcgatctt ccaatacgtc accatagtga
   103741 taatgatcca tgatatagct caaatgacga tctgattcac gatcacgttt gtaccgacgt
   103801 tgacataaca cttcaaagtc tgctgttagg cgaatcgtga tcacctgata tccgtatcta
   103861 tctgctaatt cttggagtcg tgctttttgc ttatcactaa aaggatactc ggacacaatc
   103921 actttctttc ctgcttccat atacaaatca agtgctttgt aatagaaagg ataaactttc
   103981 gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc cgatggattc tgcatagtat
   104041 tccttgattt catcgagcgt gatggcatac atatctggaa atcgttcccg tagtttattc
   104101 ataagatatg tttttcctgt accaggacta ccagccagaa gaatgaaata ctttttcatc
   104161 tcatttattt cctaagttaa ttggactttc caagttcata aacagtccaa tcttatgctt
   104221 gacaatttct tttactgctt catttgctgg aatcacatta tggcgaagag acttgttgat
   104281 ttctgtttca ctaaaggttt ctttagctac tttcgtccag ttgactaaaa gctccgttcc
   104341 aacattgaat ttgcggatcc cgttattgat caattcagga taatcttctt ctttcacacc
   104401 tgttcctcca tggataacta gggggacatc aactactgca tcgatttctt tcagcaaggg
   104461 aatgtttact tctgtttttg atttgaattg tccatgattc gtaccgatag caatagctaa
   104521 tgcatccacg cctgtagctt ctacaaattt cactgcgtct tccggtcttg tatatacctt
   104581 gtcattttca gcgacatgga ttccttcttc tgtaccgccg attgttccaa gttctccttc
   104641 tacggaaaca ccgtgcgcat gggcatattc tactactgct tttgtcttca ggatattttc
   104701 tttgaaaggc agatgggacc catcatacat gacagaagta tatccgctgt cgattgcttc
   104761 tttgatatca tcaaagtctc gggcgtggtc taagtgcaag actgcatcaa ccatttcttc
   104821 ttctgcaata gctgtcatta cattgactaa cacatgcatg ccgatatatt tagcggtatc
   104881 gacacttgtt tgaataatga tcggcgaacc catttctttt gctgcgccta gcatagcagg
   104941 aagcatttct aggttatgcg tattaaaagc tccaactgta aaattcaact cttctgctgt
   105001 ttttgttact tctttcaatg ttgtatacat cttttattgc caccttatct ttttatttgt
   105061 ttcctttgat cgttacttgt tttgcaattt gtcgcatctc atccattttg cccatgtagt
   105121 aatcgatccc ttgatcatca ccagcacgag cagcatctgc acgcttctgg ttatagaggc
   105181 tcatcagttt tttataccca tctccgatcg tctgtttatt ttccaaactc ttttctaaat
   105241 attggatggc ttgatcgttc acatacaatt cagaaaattt aagtccaact tgttcataaa
   105301 gtttcgctaa tttatgctta tcttgggtag tggaaatctc ttgtttcaat tgtgtaatct
   105361 ctgattgaat cttttcggtc gcttctggcg atagggcttt ttcttctttt gccagaactt
   105421 ttttcggttc tttttctttt ttcttaaaaa atccaaacat cattttccct actttcaatt
   105481 aaaatgcttt caaaactggg cttaagatcc aggcatataa gagtgctgcg gctactgtat
   105541 ctacatcggt tgctgtagca ttgatgaatc ccatcgaatt aaagatcgtt actagcaaag
   105601 cgggcaataa tgtaataaag aaaccatgag caataccgcc aatgatcgct cctcggcgtc
   105661 cgccaactgc gttgccaaat atccctgctg tccctccggc aaagaaattg gttagcatac
   105721 caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa catggcaata atcgtaccaa
   105781 tcgttgtcgt aatgaaacct aaaatgacag catttgggct ataaggaaag aaaactggac
   105841 aatctaaggc agggatcgca tcaggaacta atttcatagc tatccctcga aatgccggca
   105901 cgatttcacc taacagtaga cgtacaccag ctagcaatac atacacaccc actacgaatt
   105961 ggatggcttg taaaaaggca aacatgatat agttttgatc tcctgataat tcagaagcga
   106021 atggttcgcc tgcaaataaa acagtgatga tatatagcgg gaccatcacg accatgacag
   106081 acaaataagt atcttgcaga aattcgaaat gtgtcggcaa cttgatatct tcgatcgact
   106141 ttttattttc gcctttctca ccaaaaagct tagcaacacc ggcttcaaat aagtacccga
   106201 tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt gattttacga atgatcggtt
   106261 gcgctactgc cggcatagca atggcgaaac aagctccgat aaaaccaccg accaagatca
   106321 atacgatccc tcgcagaccg gcaaaatagc caaagactgt cgtcatcgtt gccatccata
   106381 aaattgcttg accagttaaa aaaatgtact tccattttgt gaaacgagcc aaaataatat
   106441 tgaatacaaa aatggctaag aacgtcagtg caatatctct acctaaaccc aaatcattca
   106501 ttgcttgacc attgatagat tcaatagaag ggatgatccc ttgcatatga aatccttcag
   106561 taaagatttt cccgaaataa gttaaacttc caacgatgat gctcgaaccg gcactcaaca
   106621 cttgaaatcc taggagtgtc tttaacgtgc cagagatgac ttgtcctgct gatttcttct
   106681 gcagtagtag acccaacata gcaatcaatg caatggtgat cgatgcctga gtcaaaatat
   106741 tttcgatgat aaaattgata acgcccatgt ttttaccctc ttttcttttt gtatttagat
   106801 aactccctta tcttgcagaa ctggggtgat tttttcttca atttcatttt ttgaaacaat
   106861 atttttcaaa tagatcatcg ccgttttagg atcaactgcg aacttttcga actgcgaacg
   106921 aaaattttct gctgtgatga tcacatctgg tttcatggaa actgcggaag aaatatcaca
   106981 atggtccagt ttggcttcta ctcctaattt gttcaaaaca tcctcagcag acatctgtgc
   107041 tgcaaaacta cttcctaaac ctgcaccgca tacgaataaa atgttcaatt tgttcatctt
   107101 tgttctcccc cgtatcattt attatttctc aactactgaa aaaagcagtt gtttgaaatc
   107161 ttcgatattt gtgcattcaa tcaatcgatc caacttctct tcgttgttga tcaattcaat
   107221 tagttctttc atgatgttca gatgcgagta ggagtctact gctgccaaac aaaaaataat
   107281 ctttactgga tccatttcag gattaccgaa atcgattggt tgctcaatcg tagctacgct
   107341 gactcctaat ttattgaccc catcttctgg tcgggcatgg gctagtgcca tgtgtttgcc
   107401 aatcacgata tacggtccgt aatcattcac tgcacgaatc atcgcttcga catagcgctc
   107461 ttcgacaatg ttttctttaa ccaatggtct agcagctagt tcaatcgatt cctcccaagt
   107521 agtggctttt tctttgatca tgatattgct gtccgtcaaa atatctttta acataggctg
   107581 gatctccctt ttgttgattt ctaaattgtt tgtttcgaat aatttttcta acttgccata
   107641 gatgattcga tctactcggc caccactttc tttcatgaca tctattattt gagtgaataa
   107701 ctccgtcgat tcatgactgt taggctctaa ccgctgtagt tcttcatttc ttttcaaaaa
   107761 gtcttctata accaagatat tcgtctcagt taaaatcgga tcgacaacta aaagcggctt
   107821 atttcgataa ttcaattgaa aggtgctgaa aaccagattg acatccaatt tatctactaa
   107881 ttcgatttct tttgaactta aaactgcagt aatctcgatc tgaggaaact tttctttcag
   107941 attctctgct aacagatttc cagtagccat cccatggtta cagacaacga ctgatttata
   108001 aacataattc aagtctcgct tgattacact ggcgatcgtc gaaaagtgga tgacaagaaa
   108061 agctatctcg tcctccagta tcttgccacc aattacttct tcgatcgtag gaacaaaatg
   108121 agtgactgcg cggtagattg ggaaataatt tgttcggatg ttttctttca atggattcat
   108181 gacttggata tgattcttga ttcttacaat cagtgcagcc atgtgcttat agagattttc
   108241 gaataaagct tcttccttta gatggaaagg tacgtgtgtc tcgttctcta caaattggat
   108301 caattggatc gttagtaatt gagcttgtac ccactcgatt gaattggaga tatctctagc
   108361 attgaacgtc tcaatagtga aacgaagata atttctttct acttgcggga cattgtccaa
   108421 ttgaaattcc tcgatcatgt ggtcaataaa tgcagaaaag ctactgtctt ttatttctgt
   108481 atttttccaa ttaatggcag agataaattc atgtttcctg attcgctgga tccagatcaa
   108541 tgtaaaaagc aggatttgtt ttttataaat atcatcttct tgtttcaaca acttattgga
   108601 atagatctcc tgtatttttt tgatttccac caaaggaaaa taatggagaa agattttttg
   108661 agccggtgtt aattgtgcat cttgattcat aaagaagtcg gtcattccca gattctgatt
   108721 gataaaatca tagatcatcg tacggataga tcgttctgaa cctttataaa ccagtccttg
   108781 ctttccataa ctgacgatct ctatcccata actgaccagt gttgctcgta accgtctcat
   108841 atcttcatct aatgtacttt tagagatttg aaattcttct tcttttttgt ttagatggat
   108901 cgggattcgg ctgtaagcaa cggctaaaac tagatcaaaa atacgttcgc gccgattcaa
   108961 aaagccgtct ttcacatctt ttatttcagt ttctaatcga gcttgctttt ccttttccag
   109021 cttcaacaaa aaaactttgt tgcgaattgt acggatctcc ggaagctttt tactgctcaa
   109081 ccagatattc gcttcaagaa cttcctgacg gatcgtccgc tgactgacat tgaactcact
   109141 tgctagatgt tttgctttta ctggatattg ggctgtcatg attctgtgaa gtatttcaat
   109201 tgttcttgtt ttcatagaaa tgatctcctt ctgatttcaa gtatatggtt ccgcttacat
   109261 aataaaaagt agatatttct gccacttgaa gtggaaagaa agtaagcaaa gcaaacaaaa
   109321 aataagccat atgctttttc atctcctcaa cgaaaaagac atacgcctat tattacaaaa
   109381 attttgccca tctatacaaa aaagaggttg tgaaaaagct gtcctgcatc aagtaataag
   109441 aacaactaaa ctaaaatagt gcttcctatt tttcgtttgg ttgttcttat tatcgcagat
   109501 gcaagctttt aaacaccatt tagtggagag gttgtgacag agatgttcag ccttgagcga
   109561 taagaagcga ttttgaaaaa tcgcttccca tattttacaa aatcgtgcct tattgccgat
   109621 aggctatctc tggcacactg tttattcaga aaaaggagct gcgacaaggc ttttgtcaca
   109681 actccgttca taaatatttc ctgtcaatcc agcataatcg gctgaataaa atcggcttgc
   109741 catctttttt ctttctcaga aaaatcaaaa taccatatct gagaaaaata ctgtcgttgt
   109801 gtcttacttc ctgagatcaa tcgcccatct tttcgatcga ctgaataatc acagcatctg
   109861 aaatcaattc ggatggcaaa actattttcg tggatctgtc gataatttcc tacttcctga
   109921 atagttattt ttttagtatg atttcttaca ccgtctgctt tgtttttttg cagaactttc
   109981 tgatgttcgg caaacagctt gtctgtataa cagcttctgg ctttttcagg agtttcttct
   110041 tcccaagcgg attggatctt catgaatatc ttttggattt ccagaaactg tgctttcttt
   110101 tctttagccg ttcctgacat gcgggacaat atctccgctt tcagcctttt cttttgtctt
   110161 ttattcttga cgatagaaaa agcagaaacc cctcctactg acaaaacagc taatgcaaga
   110221 aatcccgttt gctcataact agaaggacct gtacctggac cataataccc ataagaatct
   110281 ctgccgtaag aacgactgcc aaaataactg cccccatatg aacgaccacc aaatgaacct
   110341 ctgcccgaac ttcgaccgct agatgtacct ccaccatgac cacccgcgac agcgaatcct
   110401 tgggtcggca gagcaaacaa acataggccc actaacaaaa tgattgctaa ataattgatt
   110461 tttttcattg tcttcctcgt tatcttttat tacaaaaaac tatttttatg atagccttgc
   110521 taaaaggaag agtcaataac cagatgcata aactgttttt ttagcaattt tgtcttattt
   110581 agtcattgag taacatcgga caaatgtatg tgtcggcagt ccataattcc tctatcttca
   110641 gcatgaacaa gcttgcagac aagagatctg cgcttcctcc aggagagatt tggttttcct
   110701 gacaaaagtg caacagtgct gtaaatggac gatttggtaa agcactttga ctagcagcat
   110761 gagcaagttt ttgcatttcc actaacgttt tctgatcact gcgatgcaat acgcatgtgt
   110821 catctagcga actcatcaaa actaacaaca tatgcatttg tttctgtaca tatgaatcat
   110881 tatctagctc cttagctgct tggattgcaa gcctgatatg cggataccct aatatcgctt
   110941 cttcatatgc accatttacg ccatattttt gcttcaccct atgaccatga gtggtttttt
   111001 tatgacgata ttttgtatca ggaaaagcag caagcttgct gacatcatca agtattttcg
   111061 ataaagaaaa aggctgttcc atactgattt tttgtgcaat gacactgatc aatagcccca
   111121 ttgcccaaat cgctccttta tgggtattga tgccatttgt agcaatcagc attgcctttt
   111181 ccgcttttcg cccaatctca gcaattcttt ctcttaactc ctgatcaata gcatgatgcc
   111241 atgctatttg agccatttct tcaaaaaaag gagttagcgt cttagcagac ttaagaaata
   111301 aagcatagtc catatcggaa tgtgcacccg catccagtgc atccactaat ccaggttttg
   111361 gacttaatgt tacttctgcg atcaatgctt cctctaccaa cgcagcgagt tgctgagtca
   111421 ctgctttttt tcgttcgtca tttatcatga cactcactcc ttcttcctac tattacttgc
   111481 tgaaaccttt tttgatagct acccaaatga tatgaagtaa ccagcttaaa gcaaatgcac
   111541 aacttgccgt cattagataa gctacaagaa tcatgactcc tgaaggtaac tgcagccaac
   111601 tatctttaaa caataataac agtacttgga aaacaaatac attggctaaa tacgtacgat
   111661 aagcataaac agacaaccat ttgaacaatg gcaattttgg cgaatcgttt acaatcattt
   111721 tggaagcgcc tatgaagaca gcaaagatga tgactaaact gtataaactt tggatcgtat
   111781 tcaagtaagg tgcattcgca aagctcaagt ctcctggatg tgcgagcaat gaacggacag
   111841 aaaagaacgc gataactaag gcaacaggta agaaaagata acgtactctg tccaaatatt
   111901 gatagacttt ttcgtgataa acaaatgcgg ctaccccata aatcatgaaa ctagcaacaa
   111961 accgatctaa taagtaccag ctgtcatgat aaggcccagt caatacttgt gtttgataga
   112021 acagatacca tgcgacatat aaaatcgttg aaccaatcaa tactggccat acatattttt
   112081 tattagcaaa tactttgtaa ccgagccaaa caaaaaacgg catgaacaat tggatctgca
   112141 gcatcataac ggtataccat aaatgaggcg ctccatcacc tgtgacatat ttcagtaaaa
   112201 agcttgcagt atccgtatac ggagtttgtt gttggactgc tggaaatagc caaagataag
   112261 ccgtcgtcca tagaagatac ggaacgacta gttcaaaaaa cttttctttc aaaaattccc
   112321 aataagacaa atgctgactt gtttttacca tattataaac aattgcaaaa ataaacattg
   112381 gtgctgtgta tttaacgatg acgtaaaagg taccaagaaa agctgtattt gttgcgctgt
   112441 ggctgtttgc caaagcaaag ctcaatatcg tttgcatcat aacagccgtt acagctgata
   112501 ctttctggaa gtcacttacg ttgattttga tttttttgtt ttcaaacatc tctttctctc
   112561 tctttttctt tattaccaaa gttctttggt taatttaggt ccttcgactg ttttcaatag
   112621 aatttttttc tctggctctc tcaaatactc ttttagtgaa aaaccgcctt gccgagtcac
   112681 tacttgggta tcgaattgct caaaaaaagc aggatgcgat tgaattacct gaattggcaa
   112741 ttcaatcggt gaatttgcgt acagcagcaa atcaaaatca ctggtttctt ttactgccgg
   112801 aatcccagtc gataattcaa atcctaagct gccgccgatt ccccatttac atcctgataa
   112861 tatgtttcta gcagcatgaa attgctgata aacaggataa tccatgattt tctcttgtct
   112921 atacgattct tgttcgatga tgtcccacgg atgaatgact tgtttgacgg catgttttga
   112981 gatttctgtt gaccatctct gattgcgggc aggtccacgt aaaccaatcg gtagtatttc
   113041 tgcgtgatcc gtcgacgcac gacgaactgt tccgaacatc ttttcagtta gaacgaccca
   113101 ttcaggcaag gtgaaccctt ctatttcatg tttatcaaaa aggacaatat catgtgcttg
   113161 aatcattttt tatgcatccc attcttcgtc cattttggca cggactttat ttgtttcgac
   113221 acgtccgaat gttttggctg tttcgtttgt gtatcgattt gtcagcatcg tgtcatttgt
   113281 attgcggaca tcttgaatgg cttcattgat tgcttcgatg actttgctga cattttgttc
   113341 tgaagcagct tggtcggtga tatcttctag gaaatgatag atcgctccta gttttgtata
   113401 attattgata tcgtaagcga tagctggaac tttttcagca gctgcttcga tttcagcaac
   113461 cgaacgtttt gtcacacgtg cagcactttc tttagacatc gcttggacgg taatttcttt
   113521 attgtttaaa gcaatcaagc ggcttgattg taatccatgt gctaaaaatg cacctgaaat
   113581 agcatttcct gcaatcaatc caataactgg atgtccagct tgacggagtt ttgcataagc
   113641 agcagcacta ttagccaaag ccacatggat accgatcaat tcttcgttat agccgtatgc
   113701 ttggcttggt acatcgatca caacgatgat tggccgtttc acttttttgt ctttatcttc
   113761 ttcgtaaacg tgatccaaaa tttgtcccat gcggaaacct tcaactaaac caacttcacc
   113821 gtttcgtaca cggtacatcg ggtttttagg atcaggtacg attgatgtca cgatggcttc
   113881 ttctatgaat ttacttgatt taccataata cacagtacta atagaagaag ttggattttt
   113941 catgccagtt agtgccgcaa accaatcgta accaacactt gctgcagctg gttcatttcc
   114001 ttctgctact tctggtaagt caattgattt tgcttgatgg ttagcaattg cttcattgta
   114061 atcgtcaatg gccattggtt ttgaaagatc taaagaatca agtaatgata aataaaatgc
   114121 tgagctttca ctgcgatgcg tgtcttttcg ttcgtccatt gctgcgatga cagcagcttt
   114181 gattgcttct acactatcag tcactaattc atcaatgatt tttgattcta cccgttgacg
   114241 agatcctaat gtattccaaa tcaagtcttt gtcactagaa tcaaattctc gaacgcctgc
   114301 ttcttgttca ataacttctg gtccattcaa accgactctc gctttttttg ttgcgattaa
   114361 ataactcatt aatgctgaag taatcgacat accgccaaat gatcctacac gtcctggaat
   114421 caaaccgatg actggtacat attgttttag agcaatgatt acatttccga tttctgaaat
   114481 agataacagt ccgtagtttg cttcttgcaa acgaacacca cctgtatcta gtacaatgac
   114541 tggaaagatc tcatttcctt ttttattttc ttctaacaca tgttcaagtg cagcaacgat
   114601 ttttgcaccg gaaacttcac cgataccgcc accttggaat tttccttcca tagcgattac
   114661 tactgcttgt ttgccattga tcgtgccacg gctgacaacg atcccgtcat cactttcagg
   114721 gacgattcct tgtgctggta aacttggcgc gatgatttga tcgaatggat caatcaattc
   114781 acgtccattg tcaagtagag cgttcactcg ttctctggcg tttaattcta caaaactatt
   114841 tttcatctga aagttcctcc attgcttgcg tcaatcgtag gtacacaact cctggtgtag
   114901 caccgaagtc attgatcacg atatctgctg aaattggata acgatcaaaa aatctcgtta
   114961 atacattgtt ccatacttcg ccgaagccat cgcttcctgt acaaattttt acagtagatt
   115021 ctgttttttc tgttggaaac atcaagattt ccaaatctcc tgaaccgacc actcctacat
   115081 ggattttctt attgatcggc tgtttcgttg gatatttata agctaattgt tccatggatc
   115141 tctccccttt ttgtaaaaca gagcatacac gttctgctta ttcgtctttc ttcttccatc
   115201 tgggtaaatg gcgacaaaag gtttttctcg ccaattttat gaccaagttc tgaatttcat
   115261 tggtggttca tacaggccat ctgaccattc gatcaaatca tcgatggttt gtgcagctaa
   115321 taatgaacgt ttcgcttcac tacgtttgat gcctagatct tctggtaatt tcacgatgcc
   115381 ggctgcacgc aagctatcta gtgattcttt cgtgctagtc attccaagag gtgtaacacc
   115441 gccaattgct gcaattgctg cttgtcgttc ttcttttgtt tttgctttgt acaaataagc
   115501 aattccttct tctgtcacga tatgtgtagt atcttcgcta taaatcatca ctggtgcatt
   115561 ggctaagcct gcttgttttt gaacagctag tgcatctaat tcttcgacaa agactggttt
   115621 tttatttgaa ccatatgttt caaccatttg aacaactaat ttttggcctt tccctaatgg
   115681 atcatcattg tcgcgaagtg attgccatgc tggtgtagaa tgacgacgac cgccaggatt
   115741 atgtcccatg ttaggcgcac cgccaaaacc agataaacgt ccttttgtta cggtagaaga
   115801 gtttccttcg taatctaatt gcaaggttga accgataaac atatcgatgg catattgtcc
   115861 tgcagcttgt gataatgttc ggttactgcg catcgtgccg tctttaccga caaaaaagac
   115921 atctgggcga gcagcaatat atttttccat accaacttcg ccaccaaaac tatggatgct
   115981 ttcaacccaa cctgattcga tggctggaat catcgttgga tgtgggttca atacccagtt
   116041 cttcgcgatt ttccctttta accctagtga ttcaccataa gttggcagta acaattcgat
   116101 agctgctgta ttgaatccaa taccgtgatt caaagattgt acgccgtgtt ctgcgtagat
   116161 tcctttgatt gccatcattg ccattagaat ttgcaattct gtgatgtttt gaggatctcg
   116221 tgtgaataaa gcttctaatt gataaggttt atctgctttc acgacaacgt ctacccagtc
   116281 accagggata tctacacgtg gtaaagaatc gacaacttcg ttgacttgaa cgatcacgat
   116341 accatctttg aaagctgcag cttcgatgat tgttggtgtt tcttccgtat tgaaacctgt
   116401 gtacaagttg ccgtctttat ctgctttatc tgcagctact aaaacaacat ttggaatcaa
   116461 atcgatgaat aagcgtccat aaagttccaa ataagtatgg atatcaccta atttcattgt
   116521 gccgtcttca atcatttggg cgacacgcaa gctttgtgca ccagcataag agaaatcgat
   116581 cttgcttgcg attccttttt caaaaatatt caaatgttct ggtcgtgaga tactagacat
   116641 gatcaaatgc agatcattca ctttttctgc atctactttt tcaagacttt ctgaaagaaa
   116701 acttgcttgt ttttgattgt cgccttctaa tacaactttg tcgccgggag caatcagttt
   116761 ttccattact tctacgattt tttcagtttc tacatacttg ccattgagca agttgctgat
   116821 tttttccaat cgctgcgctt ttttttcgcg tttagaagcc cagttgcggt ttggtttttc
   116881 cataattagt catctccatt tttgatatag aaacgtggcg tcatcaatac catgttggtc
   116941 tagattgatc gtacggatcc ctttatctac accaaatttt gtatgaatca gctttgtcaa
   117001 agtattgccg gggggaaatt caatcgtcag atccattcct aattcttttg ccaagtccat
   117061 catttggttc cactgcacag ggaaagctag gttttccaac aaatctttac gaatctcttc
   117121 tgcgtcctga gtggctcttc catccgtatt ttttatatac aaacatctag catcagatag
   117181 atggacagac tgcatatacg gcttgaattt cttgaccgta tcattcatca agagacagtg
   117241 agatggcaca gggactttca gcagcttcgc tgtttgcgct tggtgccgtt tggcttctgc
   117301 caacgtaacg gataaacctt cgatactccc agataacgta tgttgcatcg gacaattttc
   117361 atttgacaga taaactggat ggtcaggatc aaatgttttt gctacaattt cctcagcttc
   117421 gcttctcgtc acacctacta ctacgcccat tgcataaccg cttggataag cttctttcat
   117481 taagactgcg cggtgataga ctagttccgc tgcattctca aatctcaaag atcgacaagc
   117541 aacagctgca caaaaagcac caatcgaatg tcctgctgca atatcaggct gaataccgtt
   117601 tttttccatt tcatctaaat aaaatactgc ttttgtcagt aatgctagct ggataaaaac
   117661 cgtatcttga tagttttctt ctttatcaac taaccgatac cccgtcacat tttctaaacg
   117721 ttccatgtac atttcaggga tatccgctaa catccctaac ttctgactgc cttgtccagg
   117781 aaataagaaa cctgtactca tgagcctcgc ctcctcagca cttacttttt ccactgctat
   117841 cttagtcaat ttctgctata ataaaaaatg ccaaaaaaag aaaaaaccat aacaaaaagg
   117901 tatggatggg atggataatt atgaaattgc ttcatctgca gtattttaaa aaagtattcg
   117961 aaacaaaaaa tgttacccaa gcagccaaag aattatttat ttctcaacct gccttgagcc
   118021 gtgcgatcaa acatttggaa aacgaattag gtgtcccttt gttttaccat agcggcagaa
   118081 acattgagac gaccgtttat gcagaagaat tttatccata tgcagtcaag acattagaca
   118141 cattagaaga aggaatcaaa ttattgtctt cggtcaatga gaagatcgtt acttccgtca
   118201 tattgtattt agaggtagcc tctgtttcta ttccgaactt ggtccgaatt tttatggaaa
   118261 agcatcctga tatccaatta acgatcatgc aacatcattt accagatgat attaccgatc
   118321 ctgtactgta catcacttcg gagatgaagc ctggcatgac caatattcct atcatgaaag
   118381 aacctgttta tgtcgccatt cctaagaaac atcctcttgc taaaaaagaa catctgcaat
   118441 tagaagatat ccaacgcacc cccatcttga tgctgtcaaa gagcaacgcc ttccgtcata
   118501 cactggatgc agcacttgaa gcgaaaagta tcgaattgac gatcagttcg acgacagatg
   118561 atcctgcgac attacgttct attgtcaagc aaggattagc tgttagcttt ttcccaaaag
   118621 tttcttggtc ttacgataaa aatgatcctt tcgtcttgcg ggaaatcaaa gatttgccct
   118681 tgacacgaac aatttattta tcttctagct tcacagaaga tcatcctttg atcaaaatga
   118741 ttgcaaaaac gatgcgggaa ttttatttag gaaaataaaa aaaaagagct gggacaaaat
   118801 tcttgtcgca gctctttttt cgaacaaacg gtgttccaga agtaatttct tagaaaataa
   118861 gccgaaattt cccaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag
   118921 ctaaacattt ctgtcacaac ctcttctcta ttgtcctaga cattcattgc gttgatttaa
   118981 aacttccatc aatcaaatct gctagttcat ctagcttttc tattttacgt agatcaatag
   119041 agatccaatg ttctttattc atgtgatagg ctggaaaata cccctctttt cctcgcaaaa
   119101 ttccgccaag ttcaggatcg accttcaaat caagaatatt gatgatccta tggtcacttg
   119161 catcatcata gattttttct tctggaacag acatcaggac cccaaaccat tttttgcttt
   119221 tttgatgtct gaatacgcaa taatcaggaa attttttaaa aatatattct ggagacaaat
   119281 tgtatgtttc attgatgtga tcgattagtt cttttttcgt tagcatctgc ttccatctct
   119341 ttcttattga ttcgctttat tcaaagatac cacgtaagaa tagaaaaatg aatacaagta
   119401 gttgctaatc aaatacttcg atatttttct tttttatcaa ctcttaaatg ttcaatccgc
   119461 ctgtgttggt cttgctacca tctactatta aaatgtttat tattgtttgc ccacctattt
   119521 tttgatttta gtggtatcat ttttcaagtt catgatgaaa ttcttatcat cagtaaatgc
   119581 tccagaaaaa taaacaatga ttgaaaaatc agttgaaact tgaccaccgt ttattttctc
   119641 cgtattctta gaatagaagg gagtctgatt gatgtattca ctaatgaaaa aaatcattac
   119701 cgaaaaagat attcgacgtc atgtttcttt agtagaacaa ttgctgaatc atacaaagat
   119761 aaccgtaaat gaattagctg aaatcatagg gacaaccgaa cgaacgattt tttctgacct
   119821 tcaatctatt cgctctcagc tgcccgaagg ctgggacatc atcagcgatc aagcaggaat
   119881 cagtttgcaa aaccagcaaa atttattaac aaatgacctt tgggagattt ttttcaaaca
   119941 atcagtcagt gtcgaactgc tgaaaaatct gctttttaca aaaaaagttg ctgtccctga
   120001 ttttctagcc gaccatgggc tttcttatgg gacattgaaa agacatgtga ctaaaatcaa
   120061 tcagcgttta gcaagttacg acttgcagat cgatttgacg aaatatacag cttgtattct
   120121 tggaaaagag cgtgcgattc gtacttttta tcatcgattg ctgattcctt tcactcataa
   120181 caactatttt ttcgaggatt attcgatcca tgaatcccat tattttcaat ttttaagaaa
   120241 cctcagtcaa actgaactag cggttgaaac agaagagatt ttcggaacat gttggttctt
   120301 tatcaatacg atccgtataa aagcgaattg ccgattagat tcttctatcc ataccaacag
   120361 cacactctcc tccctgtatg actctgcatt gaaaaaattg tatttaacgg aaggtattta
   120421 tcttaaagac actgagcttt cttttgcttc tttctgcttt ctggaaagtt ggaattacaa
   120481 caataactat ggacaagaaa ccgctcgctg cctgcatcat tctccatttt tagaggtcct
   120541 cgaaacgttt gtagaggaac tagcttcaga gctgtctctt gatcaactaa aaaaaacatc
   120601 tctgactgat aacttggctc tcctcatatt aaaatatcat gaatcgccga tattgattga
   120661 agcattggac cgtcaatacc atcgtttttt agaaacatat gatcagcaat atgcacactt
   120721 gtatcaacag aaagagaatt tactgaacga tctaaaaaaa gaaatagaaa tcaatgaacc
   120781 aaattacttt ctccaactct tgtctctact gatccaacaa acgatttttt ctatcaaacc
   120841 gaatttgttc aatgtctatt ttattttcca aggagaacct tcatggaagg cgttcttgca
   120901 gcaggaatta aaagattact taggaaaacg tgtctgcttc ttacctatcg aactaaccga
   120961 tctttccact atttcttttg aaaaaacaga tatcttgatt tccaatttcc ctctagattc
   121021 tctagatatc cctattgtat acatttcttc tattccaacg aaaaatgagc tcaatcgttt
   121081 gactgaactc actttcaaaa cttttttata gtaacggcta gcgactgtat tctctagctg
   121141 ttttttcttt tttgcgaaac agtttgttca tgatcccttt ttctatttct acaactaaga
   121201 atagaagaat cacatttgct ccaatcaata actgttgtgt catcgttaaa cttactgtac
   121261 cgatcagctg ctggccgact ggtagaaaaa taaccatccc ttgcaataaa gcaaggatac
   121321 ctaatgaaag aaaaagcgct ttgttttgca aaagaccttg gttcaatgac ggcttggaca
   121381 actctctgca attgatcatg taaacagctt gagctaagac gatattttgc agaagaatgg
   121441 tttgctgtaa gctttgccct tcaaactgca tagccaataa ataggcagga accatgatca
   121501 gtagggagac atagatgatc cgaaacaaat gataaccaga taggattcct tgctttggat
   121561 ctcttggtgg gcgcttcatg atttctggat ctggcgactc gaaccctagt gcataagata
   121621 aagtgattgt cgttaccata ttgacccata aaatctgtac tggggacaaa ggtagcgggc
   121681 gattcagcat aagtgcccaa atcaagatca atccttgagc taatgcagtt ggcaagaaga
   121741 aattgatggt tttttgcaaa ttatccaaaa tccgccggcc ctcttttacg gcttttgcaa
   121801 tcgtatggaa attatcatct cctaaaacca tatcagctgc ctgtttgctt acttcactac
   121861 ctttgattcc catagcaatt cctacatcgg atcttttcaa agcaggtgca tcatttacgc
   121921 cgtctccggt cattccgacg atttctccat ttttctgcaa agctttaaca attcgcagtt
   121981 tatgttctgg tgtcgttcga gcaaagacat cgactattgg cacttgctga atcaactctt
   122041 catcagacat aagatccaaa tcgatccctt ctaaaacttt ggcagtatgt tttaagccaa
   122101 tttgttccgc aatcgctttt gctgtgtctt tgtgatcccc tgtaatcatt ttcactgaga
   122161 tccctgcttg ttggcattcc tttacagctt tgactgcact ttcttttggc ggatcgatca
   122221 atccggcgat ccctgcaaaa gttaattggg ttaaagtctc atgcgtgagt tcctgtttcg
   122281 aatctacttt tttataagca aatcctaaaa cacgctgacc ttttctcgca aattctgcag
   122341 ctgtctgact ccatgcttct tgttctgcag aagaaagagt tgatttagca aataatactt
   122401 ctggtgcacc tttgacgtaa atcaccgctc ctttttcctc cgcatgcatc gttgccatat
   122461 atttgtattc agaactgaac ggaatctttg cttctatttt tttcaaaggt agattggctg
   122521 tttctgtgtg tcgaatcagc gcaacttctg tcggattgcc acttaaattt tctgttttct
   122581 gttcttcttt tgtttttaat tcctgacagt ttttcataat ggacaacacg caggcttctt
   122641 cttttgcagc tacatccaca actgtcattt cattttttgt caatgttcct gtcttatctg
   122701 aacagatcac tgtcatagaa cctaatgttt ctaccgaagg aagtcctttg atgatcgcct
   122761 tttcttttgc catttcatgt actccaacgg atagaatcat ggtcaaaaca gctggcaatc
   122821 cttcgggtac cattgacacc accaaagcaa tcgtagccga aagaaggata tgccaatcca
   122881 taccatatcg aaatgtcgta aaaataacta agaataggct caaacaaaga atgccttgaa
   122941 aaatttgttt gtttaatcgg ttgatttttt taatcagagg cgtcgtttgt tgttcgaccg
   123001 attgcaatgc ttgattgatt ttaccgattt ctgtcgtgtt tcctgtttct acaacgacac
   123061 cgactgcaga accagattgt acaagcgttc ctgaaaaagc catatttttc tggtcgccga
   123121 tcgattcttc ttctgtcaaa acggcatgac ttttttcaga tgcttctgat tctcccgtca
   123181 aaatcgattc ttccactaat aaatcatgtg cttcgattaa tcggatatct gctggaacga
   123241 catctccggc acgtaagaca atgacatctc cttttaccag ttctttcgca gggatttttt
   123301 cttgcatccc gtttgatagt actatcgctt cttgtcccat cagctgcttc aaaccattca
   123361 atgattcttc cgcttttctt tcttgccagt aactaacgag tccattgacg atcactacga
   123421 ataaaataat acttccttca atatattcac tagaagcaaa tttcaaaaaa gaggcaatga
   123481 tcaacacgat catcagcaga tcagtaaaat gctttgctat ttttttccat cgttttgtct
   123541 gctgccttgc ttttatttca ttatatcctt gatcagtcag gcgtttctgt cgttcttctt
   123601 gcgacaagcc ttctgttgat gtttgatagt ttttcaatac atcttctacc gtcaatctgt
   123661 accagttcat cctatcccct cctgtttaat tgatactgtt attatacgca gggaagtttt
   123721 ttcaggcgta tcgattattt caggggggtc tgaaataatt cgttttgcaa aatgtatctg
   123781 ggcctaactg atatttctat tgcttattct tttatataca aaatttaaga atacaaacca
   123841 gataaccaat aaaaaaggaa taaagcgtgt cgtgctttcg aataacaagc tgaaagcaat
   123901 tgaagtaaaa aacaccaaca aagcaagatc tttatatctt tgtgtagtgt tccgtttctt
   123961 ttctttaata aattgaacat gtgcacacaa gatcgaagac cacacaaata gaaaacaact
   124021 cgttgtcatg cctgccaaaa gctgaaaaaa caatcgacta tcagctacaa aaaaatgtag
   124081 acaaagaccg gcaaaaaata acattcctgt aattaaaata ccgtgtaaag gtacgccttt
   124141 cttagaaagc gcacaaaacc aatggggcaa acttttttct tttgctgttt gatataaaat
   124201 cctgctagta ctgtagactg cactatttgc tgaggacaaa gaagcaacca aaatcattag
   124261 attgacaatt gctgctccgc cttggcaatg gatcacttgc agcatagtca ctaaaggact
   124321 ttcttttaaa tctaaacaat gccatggaac aacaaataaa atcgacaaaa tcgttcctat
   124381 atagaaaaag agaatacgcc aaggcagaca gttgattgct tttttcaaat tcttttgcgg
   124441 atcacttgtc tcgccaacgg ttaagccgat catttcaatt ccagcaaaac tgaataaaac
   124501 cataggaaat gagactacca ttccagcaaa gccaaacggt agtaattcat cgatctgtcc
   124561 aaaattttct ttgattccga tagactgggt atgaaaatct gagacactca atcctatacc
   124621 caataagata aaaaggaaaa tccccaagat cttgatgatt gagaaaaaag attccaattc
   124681 accaaaccac ttgactgcaa caaggttgac tgccattaag atcattagtg cgctacctcc
   124741 gcttatcaaa gctggtacat gtacaaacca gaaatggata tacaatccta ttgctgttaa
   124801 ttccgcaagt ccagctccaa gccagcaaag ccaataagtc caattgacaa aaaatgccca
   124861 ctttttgcct aaataatagt caacgaaatc actgatagag ttaaatgtac tatttgctaa
   124921 aaataaatca cccatggccc gcatcaaacc ataaatagcc aaaccgcata tcaaataaac
   124981 gagaataaca gctggccctg ctgatgcaat tgcctttgct gaacctaaaa ataatcctgt
   125041 tccaattgcg ccacctaatg cgattaactg gatatttcga ttagttagag tccgtttcat
   125101 tgccggtttt tccatcaagt tcaccttttt ctttttcaag cagcatggcg acttcctgtt
   125161 cccaagaagg tttctttatt ttttcaatac agagtggtac tacaaacaaa atgacactga
   125221 tggctgtgac tgcaattaga ttgatccatg ttccagcttt catcgtcagt ccgacagaaa
   125281 atccgattgc cgcaaataaa acaataacga cagtccaaac tccaagattg ctctttttct
   125341 ctttttttcc aattctaaat ggtcgattca actttgcatg tgtataacgc aatctcaaaa
   125401 ctgcgatccc catgatgaca tagacgatac agtagataac agtagttgaa tttacgagca
   125461 tcaaaaatgc ttgattgacg ccaggaatca gcaaataaaa aagtgcaaag attgagataa
   125521 tgacagactg agtcaaaata acggatttgg atacgtcaaa ttgattcgtc ttccaaaaat
   125581 tgaattttgg tggatacgca ccttttctag cactttctgt cactgtcttt gctggtcctg
   125641 aggcccaagc agataattgg actgctacac caatgaagac aagtaaacta aacagattca
   125701 caataagatg tggcaaacca agaatttggc aataaatcga aatagattgc gcgatattat
   125761 tcaattccat ttgtcctttt ggtacgacat ttgctacaac taaagcattc aaaatattga
   125821 cgaaaaatat aaaaattaac gcggcaaata ctccgcgaat atacgtttta actggatttt
   125881 ccaatcgagt gatatacaca gaggacattt cgataccagt aaaaataaac atgatcggcg
   125941 caaaataaac aaatgaggat gcagtcgtag tatctggtat taatttgtcc catgaaaaac
   126001 tgcctaatgt tccggttggt acgatcccaa ctttgatcca cgcagcaaac cctaataaga
   126061 gcatcatcgt caaaggcacg tacaatccta accaaacacc gacttttccg ccgatttttg
   126121 ccatatcaaa tttcaaattc aaaagagtga tgatccaata aacgactaaa atgacaacta
   126181 atgtaaattt gctgttcaaa ccaagcggca cattatcgat aatattacca aacaacggag
   126241 cgagtgctga agcgaccatc accatcccag gaaacatttg gacccataac agccatgaga
   126301 caacgaaccc ccattttttt cctagagcat tcgataccca aagttctgga ccaccttttt
   126361 ggggaaacat tccagcaaat tcaccagaga tcaatgagat tggaaaagcg tacaataata
   126421 ctgcaaccag catgtaaaaa atcatcgtcc agctcgttgc tgctacatct ggaatatttc
   126481 gtacacttgc tacaagggcg caggtcattc cgataaaggt catggtcgtg atttgctttg
   126541 atgaaccagt atctttcatc tttattcctt ctttcaatag cgacatatgt ttttgttttc
   126601 ttttattttt gttcaagaac agaaatgaca aggtttcatc gattcagtac aaaacccagt
   126661 atcaccgaaa catccattga acgatcttag tgaactgggt ttgatacatg aaacgaacga
   126721 tccaacttat agaacgtttt ataaaaaaga atatcttggc gaggaatata tgaaaaacag
   126781 ggggaaatac aattgttttg atgaagcaaa taattgtctg ttctccatat gtctcttgtt
   126841 attctttcac acaaaaatca gttctattgg atgatttcgt ttagtttctt ttccattgct
   126901 ttggcgattt tttttgtata aaaattgaaa attcgatcat cattcaaata aggagtcatg
   126961 atagccgcac gtagcagagt gattttctgt tcttttttcc attcttcttt cgttagaccc
   127021 attctttcaa caaaacgaat cggagaatca ccgtaatctt cttgtgtaaa tgtagtatga
   127081 gaagtaataa accgttcacc ataaacatcg ccatccatat aagaagaccg atcgaacatt
   127141 ttttcattta attcattgat agcgttcaaa tctgtacagc cttgttcttt gaatacccaa
   127201 ttgaccatat taaaatcagg atgattcaat ggatagactt cgattgtttt tcctttaaca
   127261 gtaaaagtca gatgatcgag aaattctctg aatctttgag ctgcctcgat tgaagcccca
   127321 atcaattgac catatccggt cacattcaat ggcaatacac gatgtgctgt ccagactgcg
   127381 gcggcggttg ctccagcttt tgatccttcc aaaatataag ctcctaacat atctggagcc
   127441 tttactgatt tttcaaacac atatggcgcg aaataagaaa tgatatttct catattttta
   127501 tgtttgatta cgattcctcc agcagcataa gggacatagc ccatcttatg aggatctatc
   127561 gtgacagaat ccgcttctga aatagctctg aacccttcat atacctcttt gtcaatcgtt
   127621 acgcagtggt ggaaaacatg atgttcttca aagaattcag ctaaggaagc atacggcaca
   127681 aattctcctg cttcgtttag aaacagtgaa cgcgcatagc cgccatatgc ggcatcaaca
   127741 tgtaaataga aatagatccc ttcatctttt aacctttctc ttaattggac gattttatct
   127801 acactgtcta cttgaccttc ttctgtcgtt cctacgacag caaccacacc tagaattggg
   127861 attttttgtc cagctaattc acgaattgtt ttttccaaag cattaatatc cattcgataa
   127921 tcctcttgaa ctgggatagc aaccatctga tctaagccga ctccgcaaat atctaacgcc
   127981 ttcatccaag aatagtgttt tgtttgaggg accagccatt tccctaatct ttggatattt
   128041 tttccgctac gggaagaagc tgctttgacc tcatccattt cttcgtccgt aaagctttcg
   128101 gtcatttcca aaatttcttc aacagataaa ttcaaaagtt cccactcaga catttttttc
   128161 actttttcag gcagcacttc cttgacagct aaaggaatcg atttgatgca acgggcgtac
   128221 cataatcctt caagattagc aatagaacca tctgctgcaa tgtgtcccca tccgtctgtc
   128281 atattgaata atgaagcaaa atcctgtcct acttctgctt ccatttgcga agttgccata
   128341 gaagattcca atgcaacatt atttgggttc cacagcatcg catagttgta agctaataaa
   128401 gcaggcatca atgtttcagc attcatttgt ccccagtaac ggcctgctga atgccatggg
   128461 atcgatcctg cgcgtaatcg ctgactgact tcatccaaga cctctatcat atggtcgcgg
   128521 gtggctagat aatctggagt gagcttatct ccttcgctaa ttgcaggcat atctgaagga
   128581 atataatttt ctctccatcc aagatgttca tctaccatct tgttcaacaa cattttataa
   128641 actggtccat tttcagcttt gtcgcctata aagacggcct tgatatccat atctttcatt
   128701 tttcttccct ccatattttg ttggtcaatg tcttgcagat acatgtgttt atctgtaaca
   128761 cctcttcact atagcgccaa aaatgagaaa ttttttttta taacatcaaa tccacgtaga
   128821 tttttacaaa caaaaataaa taaagtcgga tttttctgat aattcaacgc aaaaaagcga
   128881 acaatttttc aataaatcag tcttctaaaa tagataacct aaaaaaagcg aacaaacaaa
   128941 taaaatgact gtccttcatt cactaaacat atttttattt ttttatattg ttaaaaattc
//
LOCUS       DAYYFU010000002       108933 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000002 DAYYFU010000000
VERSION     DAYYFU010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 108933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 108933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..108933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.002"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1872
                     /locus_tag="ACHA34_000111"
     CDS             <1..1872
                     /locus_tag="ACHA34_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFM8790797.1"
                     /translation="SAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQ
                     YGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQIN
                     RMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKN
                     VEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYL
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
     gene            complement(3807..4472)
                     /locus_tag="ACHA34_000112"
     CDS             complement(3807..4472)
                     /locus_tag="ACHA34_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8790798.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            4598..6634
                     /locus_tag="ACHA34_000113"
     CDS             4598..6634
                     /locus_tag="ACHA34_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFM8790799.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            6638..7276
                     /locus_tag="ACHA34_000114"
     CDS             6638..7276
                     /locus_tag="ACHA34_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8790800.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            7304..7732
                     /locus_tag="ACHA34_000115"
     CDS             7304..7732
                     /locus_tag="ACHA34_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8790801.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            8189..9100
                     /locus_tag="ACHA34_000116"
     CDS             8189..9100
                     /locus_tag="ACHA34_000116"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8790802.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            9317..9754
                     /locus_tag="ACHA34_000117"
     CDS             9317..9754
                     /locus_tag="ACHA34_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8790803.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            9849..10733
                     /locus_tag="ACHA34_000118"
     CDS             9849..10733
                     /locus_tag="ACHA34_000118"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8790804.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            10957..14124
                     /locus_tag="ACHA34_000119"
     CDS             10957..14124
                     /locus_tag="ACHA34_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFM8790805.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            14192..14689
                     /locus_tag="ACHA34_000120"
     CDS             14192..14689
                     /locus_tag="ACHA34_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFM8790806.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            14708..15172
                     /locus_tag="ACHA34_000121"
                     /pseudo
     CDS             14708..15172
                     /locus_tag="ACHA34_000121"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            15169..15927
                     /locus_tag="ACHA34_000122"
     CDS             15169..15927
                     /locus_tag="ACHA34_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8790807.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(16013..16753)
                     /locus_tag="ACHA34_000123"
     CDS             complement(16013..16753)
                     /locus_tag="ACHA34_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8790808.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            16814..18067
                     /locus_tag="ACHA34_000124"
     CDS             16814..18067
                     /locus_tag="ACHA34_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8790809.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            18389..18616
                     /locus_tag="ACHA34_000125"
     CDS             18389..18616
                     /locus_tag="ACHA34_000125"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8790810.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            18739..19065
                     /locus_tag="ACHA34_000126"
     CDS             18739..19065
                     /locus_tag="ACHA34_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8790811.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            19133..19846
                     /locus_tag="ACHA34_000127"
     CDS             19133..19846
                     /locus_tag="ACHA34_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM8790812.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            20156..21007
                     /gene="licT"
                     /locus_tag="ACHA34_000128"
     CDS             20156..21007
                     /gene="licT"
                     /locus_tag="ACHA34_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFM8790813.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            21128..22993
                     /locus_tag="ACHA34_000129"
     CDS             21128..22993
                     /locus_tag="ACHA34_000129"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8790814.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            23007..24450
                     /locus_tag="ACHA34_000130"
                     /pseudo
     CDS             23007..24450
                     /locus_tag="ACHA34_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            24558..25259
                     /locus_tag="ACHA34_000131"
     CDS             24558..25259
                     /locus_tag="ACHA34_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8790815.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            25385..25897
                     /locus_tag="ACHA34_000132"
                     /pseudo
     CDS             25385..25897
                     /locus_tag="ACHA34_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(26295..26504)
                     /locus_tag="ACHA34_000133"
     CDS             complement(26295..26504)
                     /locus_tag="ACHA34_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFM8790816.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            26745..27320
                     /locus_tag="ACHA34_000134"
     CDS             26745..27320
                     /locus_tag="ACHA34_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFM8790817.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            27320..27760
                     /locus_tag="ACHA34_000135"
     CDS             27320..27760
                     /locus_tag="ACHA34_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8790818.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            27776..28477
                     /locus_tag="ACHA34_000136"
     CDS             27776..28477
                     /locus_tag="ACHA34_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8790819.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(28965..29390)
                     /locus_tag="ACHA34_000137"
     CDS             complement(28965..29390)
                     /locus_tag="ACHA34_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8790820.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            29764..30735
                     /locus_tag="ACHA34_000138"
     CDS             29764..30735
                     /locus_tag="ACHA34_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8790821.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            30767..32164
                     /locus_tag="ACHA34_000139"
     CDS             30767..32164
                     /locus_tag="ACHA34_000139"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8790822.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            32323..32946
                     /locus_tag="ACHA34_000140"
     CDS             32323..32946
                     /locus_tag="ACHA34_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8790823.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            33695..35788
                     /locus_tag="ACHA34_000141"
     CDS             33695..35788
                     /locus_tag="ACHA34_000141"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8790824.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            35948..36814
                     /locus_tag="ACHA34_000142"
     CDS             35948..36814
                     /locus_tag="ACHA34_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8790825.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(36836..37273)
                     /gene="ndk"
                     /locus_tag="ACHA34_000143"
     CDS             complement(36836..37273)
                     /gene="ndk"
                     /locus_tag="ACHA34_000143"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFM8790826.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     regulatory      37446..37620
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            37962..40610
                     /gene="mgtA"
                     /locus_tag="ACHA34_000144"
     CDS             37962..40610
                     /gene="mgtA"
                     /locus_tag="ACHA34_000144"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFM8790827.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     gene            40697..41368
                     /locus_tag="ACHA34_000145"
     CDS             40697..41368
                     /locus_tag="ACHA34_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8790828.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     misc_binding    41445..41678
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    41721..41959
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            42015..43088
                     /locus_tag="ACHA34_000146"
     CDS             42015..43088
                     /locus_tag="ACHA34_000146"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8790829.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            43090..43770
                     /locus_tag="ACHA34_000147"
     CDS             43090..43770
                     /locus_tag="ACHA34_000147"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8790830.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            43787..44626
                     /locus_tag="ACHA34_000148"
     CDS             43787..44626
                     /locus_tag="ACHA34_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8790831.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            44657..45880
                     /locus_tag="ACHA34_000149"
     CDS             44657..45880
                     /locus_tag="ACHA34_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8790832.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            46283..47701
                     /gene="nhaC"
                     /locus_tag="ACHA34_000150"
     CDS             46283..47701
                     /gene="nhaC"
                     /locus_tag="ACHA34_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8790833.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(48205..48648)
                     /locus_tag="ACHA34_000151"
     CDS             complement(48205..48648)
                     /locus_tag="ACHA34_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM8790834.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(48866..50206)
                     /locus_tag="ACHA34_000152"
     CDS             complement(48866..50206)
                     /locus_tag="ACHA34_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8790835.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            50475..51371
                     /locus_tag="ACHA34_000153"
     CDS             50475..51371
                     /locus_tag="ACHA34_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8790836.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(51521..52462)
                     /locus_tag="ACHA34_000154"
     CDS             complement(51521..52462)
                     /locus_tag="ACHA34_000154"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8790837.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            52588..53709
                     /locus_tag="ACHA34_000155"
     CDS             52588..53709
                     /locus_tag="ACHA34_000155"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8790838.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            53967..55469
                     /gene="eat(A)"
                     /locus_tag="ACHA34_000156"
     CDS             53967..55469
                     /gene="eat(A)"
                     /locus_tag="ACHA34_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8790839.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            55536..56063
                     /locus_tag="ACHA34_000157"
     CDS             55536..56063
                     /locus_tag="ACHA34_000157"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8790840.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            56274..57335
                     /locus_tag="ACHA34_000158"
     CDS             56274..57335
                     /locus_tag="ACHA34_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM8790841.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            57338..58234
                     /gene="murQ"
                     /locus_tag="ACHA34_000159"
     CDS             57338..58234
                     /gene="murQ"
                     /locus_tag="ACHA34_000159"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM8790842.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            58248..60230
                     /locus_tag="ACHA34_000160"
     CDS             58248..60230
                     /locus_tag="ACHA34_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM8790843.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(60295..60741)
                     /gene="nrdI"
                     /locus_tag="ACHA34_000161"
     CDS             complement(60295..60741)
                     /gene="nrdI"
                     /locus_tag="ACHA34_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM8790844.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            60952..62376
                     /locus_tag="ACHA34_000162"
     CDS             60952..62376
                     /locus_tag="ACHA34_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM8790845.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(62557..63330)
                     /locus_tag="ACHA34_000163"
     CDS             complement(62557..63330)
                     /locus_tag="ACHA34_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8790846.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(63327..64232)
                     /locus_tag="ACHA34_000164"
     CDS             complement(63327..64232)
                     /locus_tag="ACHA34_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8790847.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(64386..64706)
                     /locus_tag="ACHA34_000165"
     CDS             complement(64386..64706)
                     /locus_tag="ACHA34_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM8790848.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            65180..65866
                     /locus_tag="ACHA34_000166"
     CDS             65180..65866
                     /locus_tag="ACHA34_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFM8790849.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            65868..66812
                     /locus_tag="ACHA34_000167"
     CDS             65868..66812
                     /locus_tag="ACHA34_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFM8790850.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            67011..67655
                     /gene="pcp"
                     /locus_tag="ACHA34_000168"
     CDS             67011..67655
                     /gene="pcp"
                     /locus_tag="ACHA34_000168"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFM8790851.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            67734..69011
                     /locus_tag="ACHA34_000169"
     CDS             67734..69011
                     /locus_tag="ACHA34_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8790852.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(69195..69944)
                     /gene="pnuC"
                     /locus_tag="ACHA34_000170"
     CDS             complement(69195..69944)
                     /gene="pnuC"
                     /locus_tag="ACHA34_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFM8790853.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            70274..70654
                     /locus_tag="ACHA34_000171"
     CDS             70274..70654
                     /locus_tag="ACHA34_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8790854.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            70750..71259
                     /locus_tag="ACHA34_000172"
     CDS             70750..71259
                     /locus_tag="ACHA34_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8790855.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            71336..71515
                     /locus_tag="ACHA34_000173"
     CDS             71336..71515
                     /locus_tag="ACHA34_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8790856.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            71570..72133
                     /locus_tag="ACHA34_000174"
     CDS             71570..72133
                     /locus_tag="ACHA34_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8790857.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(72180..72695)
                     /locus_tag="ACHA34_000175"
     CDS             complement(72180..72695)
                     /locus_tag="ACHA34_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFM8790858.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(72721..73590)
                     /locus_tag="ACHA34_000176"
     CDS             complement(72721..73590)
                     /locus_tag="ACHA34_000176"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8790859.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            73716..74396
                     /locus_tag="ACHA34_000177"
     CDS             73716..74396
                     /locus_tag="ACHA34_000177"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8790860.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            74549..75529
                     /gene="pta"
                     /locus_tag="ACHA34_000178"
     CDS             74549..75529
                     /gene="pta"
                     /locus_tag="ACHA34_000178"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFM8790861.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            75652..76125
                     /gene="tsaE"
                     /locus_tag="ACHA34_000179"
     CDS             75652..76125
                     /gene="tsaE"
                     /locus_tag="ACHA34_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFM8790862.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            76118..76642
                     /locus_tag="ACHA34_000180"
     CDS             76118..76642
                     /locus_tag="ACHA34_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFM8790863.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(77132..77665)
                     /locus_tag="ACHA34_000181"
     CDS             complement(77132..77665)
                     /locus_tag="ACHA34_000181"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM8790864.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(78529..79281)
                     /locus_tag="ACHA34_000182"
     CDS             complement(78529..79281)
                     /locus_tag="ACHA34_000182"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFM8790865.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            79394..80314
                     /locus_tag="ACHA34_000183"
     CDS             79394..80314
                     /locus_tag="ACHA34_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8790866.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            80387..81322
                     /gene="murB"
                     /locus_tag="ACHA34_000184"
     CDS             80387..81322
                     /gene="murB"
                     /locus_tag="ACHA34_000184"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFM8790867.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            81620..83098
                     /locus_tag="ACHA34_000185"
     CDS             81620..83098
                     /locus_tag="ACHA34_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8790868.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            83375..85435
                     /locus_tag="ACHA34_000186"
     CDS             83375..85435
                     /locus_tag="ACHA34_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFM8790869.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            85518..86087
                     /locus_tag="ACHA34_000187"
     CDS             85518..86087
                     /locus_tag="ACHA34_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8790870.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(86262..87068)
                     /locus_tag="ACHA34_000188"
     CDS             complement(86262..87068)
                     /locus_tag="ACHA34_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8790871.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            87206..88720
                     /locus_tag="ACHA34_000189"
     CDS             87206..88720
                     /locus_tag="ACHA34_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFM8790872.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(89123..90106)
                     /locus_tag="ACHA34_000190"
     CDS             complement(89123..90106)
                     /locus_tag="ACHA34_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFM8790873.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(90111..90758)
                     /locus_tag="ACHA34_000191"
     CDS             complement(90111..90758)
                     /locus_tag="ACHA34_000191"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8790874.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(90843..91475)
                     /locus_tag="ACHA34_000192"
     CDS             complement(90843..91475)
                     /locus_tag="ACHA34_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8790875.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(91491..92042)
                     /gene="coaC"
                     /locus_tag="ACHA34_000193"
     CDS             complement(91491..92042)
                     /gene="coaC"
                     /locus_tag="ACHA34_000193"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFM8790876.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(92039..92776)
                     /locus_tag="ACHA34_000194"
     CDS             complement(92039..92776)
                     /locus_tag="ACHA34_000194"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFM8790877.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(92773..93762)
                     /locus_tag="ACHA34_000195"
     CDS             complement(92773..93762)
                     /locus_tag="ACHA34_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8790878.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            94020..94562
                     /locus_tag="ACHA34_000196"
     CDS             94020..94562
                     /locus_tag="ACHA34_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFM8790879.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            94579..95664
                     /locus_tag="ACHA34_000197"
     CDS             94579..95664
                     /locus_tag="ACHA34_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8790880.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            95676..96482
                     /locus_tag="ACHA34_000198"
     CDS             95676..96482
                     /locus_tag="ACHA34_000198"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8790881.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            96483..97310
                     /locus_tag="ACHA34_000199"
     CDS             96483..97310
                     /locus_tag="ACHA34_000199"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8790882.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            97307..98380
                     /locus_tag="ACHA34_000200"
     CDS             97307..98380
                     /locus_tag="ACHA34_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8790883.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            98808..101504
                     /locus_tag="ACHA34_000201"
     CDS             98808..101504
                     /locus_tag="ACHA34_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8790884.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            101569..102069
                     /locus_tag="ACHA34_000202"
     CDS             101569..102069
                     /locus_tag="ACHA34_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8790885.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            102081..102575
                     /locus_tag="ACHA34_000203"
     CDS             102081..102575
                     /locus_tag="ACHA34_000203"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8790886.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            102596..103405
                     /locus_tag="ACHA34_000204"
     CDS             102596..103405
                     /locus_tag="ACHA34_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8790887.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            103402..104223
                     /locus_tag="ACHA34_000205"
     CDS             103402..104223
                     /locus_tag="ACHA34_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8790888.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            104274..106373
                     /locus_tag="ACHA34_000206"
     CDS             104274..106373
                     /locus_tag="ACHA34_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFM8790889.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            106370..107488
                     /locus_tag="ACHA34_000207"
     CDS             106370..107488
                     /locus_tag="ACHA34_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8790890.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            107527..108405
                     /locus_tag="ACHA34_000208"
     CDS             107527..108405
                     /locus_tag="ACHA34_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8790891.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            108551..108748
                     /locus_tag="ACHA34_000209"
     CDS             108551..108748
                     /locus_tag="ACHA34_000209"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8790892.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            108732..>108933
                     /locus_tag="ACHA34_000210"
     CDS             108732..>108933
                     /locus_tag="ACHA34_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8790893.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVV"
BASE COUNT        35813 a        18619 c        22600 g        31901 t
ORIGIN      
        1 agtgctgaaa atatggaaga aatcaaaaaa atgtttccga aatccacttg gtttggcttt
       61 acaggtacac cgatttttcc tgagaatcgg aaacaagcca aagggcaatt ggcacgtaca
      121 acccatgatc agtatggaga ggtcttgcat acctatacca ttaagaatgc gctagaagat
      181 ggctctgtct taggatttca agtagagcat gaaaacaccg tggagccgac ttcactagag
      241 aataaaatct atcggaaatt aaaagaagta gaaacatatg cagagtattc atcagaacag
      301 atcaatcgta tgattgatca aatggaacct gtcaaaaaag aaagttattt ggacccagct
      361 gtttttgaag cagatgaaca tatccaaaaa gtcattcata aaatgttccg tccagataat
      421 gcctatacaa aatttgattt tcgaaatggc aggccaacga aatctgcaat cttaaccacg
      481 agttccattg acatggccaa aaagtattat cgggccatca aagagatgac gaaagagcct
      541 gattggttaa caaaagagtt tcccaatcag ctcattcgtg aaggacgaac gatggaggat
      601 cctgattttc cacgaatcgc tattacctat tcattagaag aaaatagccg agattcaagt
      661 gcccaacaag aagaaatgca aaaaattatt gaggaatata atcagtatta tgatacttcc
      721 tggtcattag cagacattga gcgttataac ggagacatca ataatcgttt ggcacgaaaa
      781 agagcggaat tcaaacaatt tgggaaacaa atcgatttag cgattgtagt ggatcgtcta
      841 ttaacaggtt ttgacgcccc tacgattcaa accctatttg tggatcgtaa tctagagtat
      901 gcaggattga tccaagcttt ttcacgtacg aatcgtacgt atcctgataa gacaaaagga
      961 ttgattgtga cgtttcgtaa gccagcgaca atggagaaga atgtcgagga tgcgacaaaa
     1021 ctgtattcaa aagcaaaaga agaatcaggt ttgatttatc caagttatca ggaatccaaa
     1081 aaaagattca aacaagcata tggtaagtta aaagaggttt catccatgga atccattgat
     1141 gaacatacgc cacttgaaat ccgtatcgag tacgtcaaag catttcaaga attgaatcgt
     1201 gcttatgaat cgttagtgac ttatgacgat tacaatgatg acatggtgaa atctccaact
     1261 ttgaacaatc aaattcaact tttggaagaa caaattggcg tgtacgagac cgtgaagggt
     1321 tcattaatag aggaagaacc agaaaaagaa ggagaagatt tttcaacgat cgaattttat
     1381 agtgaaaata ccactaagtt gtatgatatt gattcaactt atattgatca attactagga
     1441 acttatgctg cgaatagttc agatgcacga gaagaaattg aaaaagcttt ggctaaattg
     1501 aataaaacag aaggagtcaa agaagtttac cgacaaattt taaatgcatt tgatgtgggg
     1561 actttagata gtaatgaaga tatctttgtc attaaacgtc gctattttac acaagcgagt
     1621 gatgatttga ttcatgtctt ttccaatgag tggtttgttt ctgagaaaga attacatgct
     1681 tctaatctgc aatatctacc aggtgaagat ccaattccta atatgaaagc aattatcaac
     1741 agtaaggatt acgaaggata taaagccaaa catccagaag cgaagccatt taaataccca
     1801 caagaaatga agcgagcatg gcgtaaaatg ttagatgatg agttgattcc acttgagaat
     1861 gaattgaggt aaacgagaag agttaagaaa attgtacgat tcaaagatgg tagtcaagtg
     1921 gaagtgtaga aaatagggat agatgtcttt tatttgttca cattttataa cgaagtattt
     1981 aagacagcta ttgaataatg tttcctccac ctttttcatt catttacccc accccgttgt
     2041 tatatgaccg tgttttgaga ggaaccaata aaaagcatag gctaactagg ggttcctttt
     2101 tcttttgttt ttttggaatc cgcttaagtg aaatgtatct gaaatgaaac ggagataaag
     2161 tgattttttg ctataatgac aaggttttag ctgtttcaga taccatgtaa cccaaaatag
     2221 tttattgaga aaataggaga gtatttgata ctaactagta ggaaaaaatg agataaaaat
     2281 taaatggaat catttcaggt aatctcaaat aaaaaaatat acagtcctgt ggcactttac
     2341 ctatgggact gtatattttt tactctcagc gggcttgttt tgactccgtg tctgactccg
     2401 tcgtattgat tttaaatgat attcgatgac catttaacag gtcatttttg aaatttttgt
     2461 ctctatatga ctcagtttga taattattga tagatcaatt tgggtctata tcgaacaagg
     2521 gaataatcca ataaaaaatg atttagaata tcaaaataac aaaaaagagg actacctgta
     2581 ttcgtcctct tttttgacca gattttgacc agtctaattt acattctaat atattcatat
     2641 gagctaattt tttgattaaa tggtaagttt ttccttgttt gttttagttt aaactagtgt
     2701 gtaatttaaa aatctatatg gaagggtctg tggggtaata cttaaataaa ggctaggaac
     2761 ctgataacat caaggttcct agccttttta taataaaatg aagcgaaaat aatttctttt
     2821 aaagagccga gattatcagc tatataagag tgaatttgat gcaaaaaaca gagccgaaac
     2881 agtcttggtg ttcgtagctc tgcttatatg tttcgaattt aagacaaaga gttggcaaaa
     2941 gatgttgata aggaagataa gataactgtg ttatataaac taggtaacta actgtaacaa
     3001 gctaatttta agagattctt attagaagac gtttaggaat ataactaaga aaatgattat
     3061 acaggtaatt gtcatttcaa taatgttttc taattttcgc gtctttgttt taagaaaaag
     3121 gccagaatca gttggaaaaa agttcgtacg atagtgaaga ggggcaaaat ttcctactaa
     3181 tattcccact aacaagagat aaaacggcca ggttggtgtg attcctctat aaaaaaatgc
     3241 ccatgctacc gaaatcccat agattaacaa ctgaacaatt tttacaatga aaaaattgtt
     3301 ctgccattac tcaaagtctc ttcctccttg tatcgtttat ttgtttcatt ttatcattat
     3361 gataggcgct tgaacaatcc attttgtatt ttattgagta ctcttggata aactaccagc
     3421 gattatcata tttatttatg ttactgtaag ccatcatata aaagtaaatg agcagatcat
     3481 agaaatataa aaaagaacta aaattgatct aaattatgtg cagcttgact acgtaccaat
     3541 ctatattact tactagattg gtcgttaatc tattcggatc gaatgagcaa aatatttttg
     3601 tgaatgtcca tccttgatta tgattccaaa aagaatcatt gggaggctgt ggcgtaaata
     3661 ttatactata agccagaaat acctatcatt ctgaactttt ttatttagga actttcatct
     3721 caaggctttt tcaattaatt tgtacccctg ttttttggat aagatcatgt gcattgattt
     3781 tttctatctc tacgttcatc tcctacttaa tgcctatctg gctcctgagg gcttcttttt
     3841 aaggcgtcgt catagtcttg gctgaggatt tcttggtaat ctgagggaat ggcttcccaa
     3901 gtgagcaggc gaacgttcat gacgtgtttt cctttgtgtt tgattacagc atggtttttc
     3961 tctttttcta cctctccagc gtgaaaagac agccatgtac catggccaaa gatatcatta
     4021 catacccaac gttcgatgga attgtccacg tatcctacag ggagtgtggt atagccgatt
     4081 tgtggtttca aaaaaggatt gaatcgatcg atcgtttcat ttttgtattg ataattgata
     4141 agaaaaagat agaccaaaca aaagacgagc agaaagaaag atagtgtctt tttgagtgag
     4201 aagggttgtt ttgatgaata agatttcttg ttaacaagag gttcatctgg caatgttggt
     4261 tctattgatt cggtagctga ttcaatagaa ggaaaatcaa tcggcagacc taaagtatga
     4321 tgtaaaagtg acagcatttc aaggtctggc attgtacgag aattttccca actagaaatc
     4381 gttttatcgg aaacaccaat tttttcgcct aattcttttt gcgtaagctt acaatttctg
     4441 cggtaagttc gtattttttc tccggtgttc atcttcttct cctttcgatt ctacagtata
     4501 tagatttatt ctacatgacc tatacacttg atagatacgt ttttttgatg aaaaacggta
     4561 tgattttatc aaagaatcat ttaagtaagg gagagtggtg ataatcaaaa aaaagttcac
     4621 agttttactc acaatcatag tgaccggtat catgagtggt tttgccttgg ttagtataaa
     4681 taaacaggta ctctatcgga caaatgaatt tttccatcaa acagatactt atactccgat
     4741 tcgattgcca caacatttta ttgggcaaga agaatatgag cagctaatca tggtatttga
     4801 aaatgtctca tcagaaactg agctttctta tatgaaaaaa acagtaaatg aatccaatga
     4861 agtagataca tcttggaact tcaagataca gccaaagctt gaaatgacat tgtattatca
     4921 aggggatgtc aaatcttctc acgagaaggt accctattct aatacctcct ttcgcgtaac
     4981 gtccattgaa gaatcgatca aaataaaaga ttttgaaggt gaatttttct tgatgacgag
     5041 agaaaaagaa gcctatgaac ggttcatttc actattcact gatcgattca atcaagcatt
     5101 tcacactgat tatgccacta atatttttga agactttgaa aatagctatt cgtattcggt
     5161 tatttcaaat acagacgtat ataattatcg agtgtattta actattggat tgattctttt
     5221 attccttttg ttatcatcct ttttcttatt gatgaataaa gaaatcagtt tattgttgaa
     5281 taatgacttt tcattacatc aagtattgtg gtgtttattg gggaaaaaga aaatcattat
     5341 tacattcata attgcaggaa tcattggtgg ttttattttt atgtattata aggagcaatt
     5401 tctttatttg ttccagatgt acgttttctt gttcagcgga atatatggta tctctttgat
     5461 ttctctttta ttagttgaaa aaatcgcttc gttaagagaa gcgttcaact atatccaata
     5521 cattcttttt acatttggat tgttgttttc tcttcttttg accgttgcca atgtcgaatt
     5581 agcaactatc gttttttctt cttcacagtt agctgtttcc catgaaccac caaaagaaat
     5641 cgctggtgag gactatcatg tattttatcc agtaaccatc gggaaaaacc acgtggattt
     5701 tatttattcc aatcgttttg catcagcagc agatcaggaa ttatatgaaa ctttgaataa
     5761 aaacggaagt atattagtca atgtatcaga ttacttgaat gaagaaaatg aacagtttgg
     5821 cagagggata aaaataaatc tgaattatct taagaaattt cctttgattg atgtctctgg
     5881 aaggttgatc caaataaccg aaaaagaaac gcaccctgtt atattgatcc ctgaacgata
     5941 tagacataca gatttaaaaa atgacctggc gcaaataaca gaatattatc aagaaatcag
     6001 tgaaaaagaa ccgaaatttc tatttataaa aaacaatcaa cccatttata catttattcc
     6061 ttctatcccc tggatcgaac attatccggt tgttgaaata ctgacattga aaaacagtat
     6121 gtatttggaa agaaatattc tcagcggaga aatctatcca ccgttaaaga tcaagatagg
     6181 ctctttgtcg aaagaaaggt tatttgagct gattgaagaa agtcagttac gtgataactt
     6241 gcagttatca tttccgtata ctcagactga agagatagct gtcaaagtgt taagcagatc
     6301 atttcattac ttgatacaga tatttttact cactttcttt tctttcttct tgttgagcta
     6361 tgtcatgtta tgcatatatt ttgtctacaa ttgtcggaaa atcgctatct ttcgttcaag
     6421 tggttattcg ctctttgaaa cgtataaaga tttcttcatg atgaacctca tcaaatgggg
     6481 aacgacaagt gtaatcttcc tttttcttat agaaagagag cctaaatatc tttttaatat
     6541 ttttttcttt agcttcattg attttattct ttcagttgtt tttattttgt ctacagaaaa
     6601 gaaaagtcag ttattgctga tgaatggagg ttaacaattg gagccgttga tagtgataaa
     6661 aggtgcgaaa aaaatcattc gaaacaaaac gattcttcat ttggatcgtt tcactgcgga
     6721 tgctggggat cttgtgactg ttgttggaga aagtggagca ggtaaaagta cgttattgaa
     6781 tatattagga ttgaatgatc agttcagttc aggacaattc tatttgttcg atcaagagac
     6841 gaaacaatta actgggagag agaagcgctt gataaaaaga aataccatat cttttctttt
     6901 tcaagatttt gggttgattg aatatgaaac aatcgatttt aatttagata ttggattgaa
     6961 gttttcgaat ttaaatgttt cagataagcg aacagcaaag tatgaggcat tgaaaaaagt
     7021 aaatataaac aagaagctaa gtacgcctat ctacacatta tctggtggtg agaagcaaag
     7081 aatcgcctgt gcgagagtct tattgaagaa tagcaaaatc attttagccg atgaaccgac
     7141 tgggtcattg gataaaagaa ataaggataa agtagttgaa atcttatcgc aacaagcttc
     7201 ccaaggtacg ttagttatcg tagtcacgca tgatccatat ctgaccaaaa taggaaacaa
     7261 gaagatcatg ctttaaaaat caaaaaggaa aggatgtcgt tttatgaaaa aagcagttat
     7321 tatttgtgca ctagggatag gttttgttgg aggaaacgct atgtcatctg aagtggaagc
     7381 aaattcagtt cacggtgaaa tctattacaa atctttaaag ccaggagaga ttcattttga
     7441 tgctaaaggt aatattatta agtcaaattt accagtagac cgggaaattt ctagaacagc
     7501 aattaatcat agctctggaa gatgggttta ttatagtgat acaacttgta cgagaaaaac
     7561 aggtcattct aaccactata gtcgtgtcta taatcgtcac ggtgcaaaag cgaaagttgg
     7621 aaactctatt cgatcagcaa atgcaacaaa aggagtatgg gcctatgcaa gtgcgacagg
     7681 aaatagaact gacacattct catgttggta caatccaacc ggttggtatt aagtatccaa
     7741 aagtttatat aaaatagaaa aaacatataa tgaaacagcg ttaaaagtta tttttggtag
     7801 ttttaatata ttcattttta gttgtataaa aatatatcat aataaaaaca acaaaaaaat
     7861 gtctggaaaa tcgaacataa aagtgtctga tttgaatatc ccagcagcgg aagaagaaaa
     7921 acaaaatcga taacatttgt tctaagaatt agttacgtta aatacggaac gcaagataga
     7981 cttgaaaaac ggtaacaaaa gaagtagtat attaaggtgt gatgtgctac ttgacattta
     8041 ttctgctgag cgaactttct ggattgtgta gccttatatc tattcaggac gtatcgagca
     8101 gattagtaaa ggtgttcttg atgattttca tcttgaacac ctttgttttt agcaattcgc
     8161 aaaaaacgaa aagtaggaga attgataaat gaaaaaaaga tttttagcac ttgccatcgt
     8221 actaggcact ggcttattgt caggctgtac gaatgccggt gaaaaaacag ccgttagtta
     8281 taaaggcggt acaatcagcg aacaagaagt aatggatagc ttgaaaaaaa tgcaaggagc
     8341 agattccgct gtgcaacagt tgatcgttta tcaagtattt gaagataaat atggagatga
     8401 cgtatcaacc aaagaaatcg attctcaata tgatcaaaca aagaaacaat tgggtgattc
     8461 atttgattca caattgaaat ctgcaggtta cacagaacaa acattcaaag acagtatcaa
     8521 acaaagtcta gcctttcaag aaggtctaaa gaaacatatc aaattgaccg atgaagattt
     8581 aaaaacggct tgggaatctt tccatccaga agtggaagct caaattatcc aagtagcaag
     8641 tgaagatgat gcaaaagatg tgaagaaagc tgctgataag ggagacgatt tctctaaatt
     8701 agccaaagac aagtcaactg atactacgac aaaagaagat ggcggaaaag tgaaatttga
     8761 ttccactact acaacggttc cggcagaagt caaagaagca gcctttaagt tgaaagatgg
     8821 tcaagtttct gatgtgatca cttctaccaa tgcttcaact tatactacgg aatattatgt
     8881 ggttaaaatg gtgaaaaacc aaaacaaagg caacgacatg gataaataca aaaaagaatt
     8941 gaaagaaatt gcaacagaca caaaattaag tgacagtacc ttccagaata aagtaattgg
     9001 cgaagtattg aaagacgcta atgtaaaaat caaagacaaa gactttgaaa atgttttgag
     9061 tacattcact tcagattcta gtacagcaag tagtaaataa gcggtataaa taaaaaatat
     9121 gaactattta aatcaataaa actgttcatc taaatctttt tattagaact aaatcaacag
     9181 tagtttaatt actacttgat ttagttcttt ttattttaaa aatttttttt acttagaatc
     9241 cagcgaccct agatattttt ctgtataatc ttaaataaag aaaacaatat caaaaaatgc
     9301 acgtagaagt agataaatgg agtggttttg caaatttctt ttatttctag aagttattca
     9361 cgtatacgga tttgtttttc tcacaaataa aaaggcagga agtacaatgg atcatttgta
     9421 tataaaatat attcatcaat tttccgtttt ggtgttaggc acactaatta tagcggggat
     9481 tgtcgcattt attattcaag gccgtgacat attaacagta catcaagagt ttttcagtta
     9541 tgcatttggt attcctctga tcgctataag gtctgataac aaaaagtgga agaaacgcta
     9601 ttacattctc ttgctatttt tgtgttttta tctgccacct aagattttta ctttattagg
     9661 tatacaatgc ctatcatttt cttttatttc ctatatcatt gttttaattt tcctcagttt
     9721 catagtatgg atgttttatc ttgtcaaaaa ataaatgaat gtttcatgaa cagtacttca
     9781 tcacagccta atttttttct ttatgctatc gttatattaa agaaatcaat cagaagggaa
     9841 agaaagtgat gaaaaaagtt ttattagtgg taaaccccag ttcaggcggt gaacaagcaa
     9901 aggaatttga gcaattagcg atagcaaaat tggaatcggt gtttgatgaa gtggtcgtct
     9961 tgcatacaaa aaaagcaggg gatgcaaaaa actttactcg cgaagcggct acggaagggt
    10021 atcatagtgt gtttgtcatg ggcggagatg gaacagtcaa cgaaggaatc agtggaatcg
    10081 ctgaacaaga acatcggcca aatttcggat ttttcccatt gggtacggtg aatgacttgg
    10141 ctcgagcatt agggataccg ctagaacctg aggaagcgat caatcatttt tcgattgaat
    10201 cagtcaagcc attggatatt ggaaaaataa atgacgacta ttttatgaat gtcgtcgcaa
    10261 ttggctctat ccctgaatca atcaatgatg tagatgcgga gaagaagacg aaatttggaa
    10321 aactagctta tttcatgtca ggaatcaaac agttagcttc cacacaatcc tactcgtttc
    10381 acgtagaagt tgatggaaag aaggaagaaa ttgagagcag taccttgctg ataggactaa
    10441 ctaactcagt cggcggtttt gaaacattac ttccaaatgc aaaagtggat gatggtaagc
    10501 tccatttggt ttatttaaaa gattcttctt tgatcgatac gcttaaaaca ttgccagacc
    10561 tattaaaagg agtagatgaa tctacgaaaa atctagccta ccttacttgt gagcaattag
    10621 ttgtgtcatt agctgatgag gaagaattga cgacgaatgt tgatggagat gaaggggata
    10681 aattacctgt gacaatcaaa gtattgccct cacatttaca ggtttattgt taaaatggag
    10741 gttgtgaccg aggagttggc gggagaacac cgcttgttcg ggataaggga ctgtgacaag
    10801 acttttgtca cagccttttt tttgtacaaa aaagaaattt acacctaata gaaaaatttt
    10861 tccataaaaa tacatatttt aaatatgaaa gtacaattgg aaatagatta aaacgataaa
    10921 ataaaattgt caaaacgtca gagaggatgg tggagaatga atcaatgtgt aaaaaagctg
    10981 attgcgtcga ttttgtttgt cttccctctc ttgatggtga catccgtttt tgccgatgta
    11041 gagaaaacaa acgaatcaac gcaatcagag aatcaaagtt ttgacaagac tactttttca
    11101 gaagaatcga ttacttctga aactacagaa tctcaaagtg aggcttctac taataaaagg
    11161 caagaagaaa ataagacaag tgagacctca gaagtagctg aacaaacgtc tgatagcagt
    11221 gaagcggcac tggcaagagc tgtttcagcg gatgggtaca gcgaagctgc cacaacagaa
    11281 gaactggcgc agcttctcgc agacgaatca gtaactaaga tccgactgat tcagcctttg
    11341 actttagaca gggaattaga gatcaaacga gatatcgtta ttgattttgg tggattcgct
    11401 cacaatttcg gcacacacca tatttatatc aatgagatac ctcagtcgat cgagttccag
    11461 aattttaaag gaacagctgc acatccaggt ggattgacac caaatatcga tggaaatgcc
    11521 attatcattg cttatatgac cgatttttgg ggaagcagat atcattttac gggagaaata
    11581 aaatttaccg ggacacttga tttgtatgat gggtccaaat taggactgat ttacgctcct
    11641 cgtgcaactg tcacattaga tggtgtttcc ggtgtgttgg atgtccaacc agtgaaggaa
    11701 ggactgaatg ccgctccggg taaagcctat ttttgtagaa gctatcaatt gaatgtgatc
    11761 aatggtagtc aactttatgg accgtatcta gggaaattct acggttttct agattcagga
    11821 gatgagactg gtagtggaaa aacagcgcca ggaatccata tcttaagcgg ttcaaaggtc
    11881 tctcttgatt atgatcgggc agatggcgtt agcgatgaag gagaggcagt cgatacgcta
    11941 cctggtaatg tcatgtttga agtcagtggt tcgggaagcg aattttcggt aaatacaaag
    12001 atcaatatta ccaatgacaa caatcgcgga atcatccaaa tgagaggttc aggatcgaaa
    12061 gtcactgtat cgaatggtgg gaaaatcacc atcaatacag caactacggg cggatttcgc
    12121 ttgcaaggag attctagtca aatccatgta tcaagtggtg gacaaatctc ggtcaaaatg
    12181 gctggagatg acgatcaacc cgggaacaat gggatgcgtt ttgtaggaaa aggcctttct
    12241 ttgatcgtag aaggagccgg atcagttatc gacatcgata agcagagtgg gcgttccagc
    12301 gcagttcgtt ttgagaatgg tacgcaaaag ctgacagtaa ccaatggagg ctcattgagg
    12361 gtaagaaata caggtgatgg caaatcttac gtgaacagac tcaatcaagg aaatcaagcg
    12421 atccaatttt atgacgcttc tggttttcta gaaagtccag gttcagctga ttttacagtc
    12481 actggagaaa aatcaaatat cgatatacgc gcagattccg gagcaactgt agatacaact
    12541 ggagatattg atttaaattt tacggcaggt gaaaaaactt atttagtcat ggtcgggaaa
    12601 acaggggaag ataagaccgg catcatcagt ggggatgtgt tgaatgtgga cttagaatct
    12661 cctacctatt ttgatttcca aaacaaacgg attggaagcg agaccaatgg cataggcggc
    12721 tgggtattcc aaggaaatgg agattcttct ttaaagatcg cccattcaga aatcgcttta
    12781 tggcgaaaaa caggcgcaac attcgataac tttgaaaaag acccggatta tttttctgat
    12841 atgacagact tatcgatcaa tgggaccgac ttaggcaatt tcgtatcaag ccctagtgca
    12901 ggtttaacaa atgaatttgc tcagacaggt gccggaatga aaaattataa tcgaatcagt
    12961 gccaataatc agctcccgat gattgaatcc ttaagagtgc caacgaatgc agataaaaaa
    13021 atctatgggc atgtcgttgt tcccgaaggt gtcgaggcaa agccaagaga tgcttggaca
    13081 ggcgaagtag aagtgacttt acgtattact tacgcagata cgacgaaatc atctgtagaa
    13141 attactgctc tcacgcaagg acaaacagat gaaaatgaag gggcaggata taacccatgg
    13201 ggagaaggcg aacaaggcgg cttgttcgaa gttcaagtac ccaatgatga gagattaaaa
    13261 acggaggatc aagtgcaagt tgttgctgct aaaaaagtaa gaggtggcca agaagtgaca
    13321 gaactggact ctgccaaaac gacagtcgat gtgattcctc ctgaacctgc taatttaagt
    13381 accacattga tcaatgcagc ttctcgcacg atcacaggta caggtgaagc aggagccttg
    13441 gtttcattga aaaaaaatca agctgttttg tcagaaacaa ctattgatga tacaggggaa
    13501 tttgttcttt ctatcccaga aaatcaactt tttgcaggag atgtattaga aattgtatta
    13561 aacgaccaag caggagaagc tactgctaag ggtgtgcaga acaagccaag taccaacgat
    13621 gaaattggga atcacaatcc aagtgatacg ccagttgttt atcatgatgc cccgcctttt
    13681 gcacctgcta cgaagatcgt agtagaaggt ggtctctcat ttcttgcccc ttccaaactc
    13741 gattttggtc agatcaaagc aactggtttg acacaagaag catttggcca aatggatata
    13801 caggaaaaat tatggattta tgatcagcga gaaacgaaaa atccgtggat cttaacgata
    13861 aaattatctc agcctttcac aaatgatgca ggattttcta tgcaggatgt cctatatttc
    13921 aagaaaggag aggagtatca attagtgaat gaagaagaac tggaaattgt cagaggacaa
    13981 aattcaacga atgtgcaagc agattattca gaatatttaa ataacggtcg ggcgtttaaa
    14041 cttgtcttaa ccaaagaaaa tcagatagct ggtaattact cagcagagat aacatggaca
    14101 ttagcagatg cgccgacaga ataaagatca aggaggatga ttgatgaatc gaatatggaa
    14161 taaaagtcat cgaaacaact gctctttact tatgatcctt ctccttggat tcatcctttg
    14221 gcagacacct gtccatgcct ccgaattcaa ttttgcggta gattttgaat taccggctaa
    14281 tcagattgat aagaacaaaa catatataaa tgtaaggatg gagcctggga aaacacagga
    14341 aatcccctac acggtcttta accgaacaga taaatcagta acagttgaga cattgatcca
    14401 ctccgctacg acgaattcaa acggtgtgat cgaatatgga gaatcaaaaa gtaaatttga
    14461 tgaaacatta agttatgata tgaaaaactt agcaaaaaca gtgggagaaa taactgttcc
    14521 tcctcaaagt gcggtcactg agacactttc actgaaagcg ccagaaaaag agctggctgg
    14581 tgtggtcgca ggagggatca cgtttcaaga aaaaaaagag aacgtttcta acaaaagtac
    14641 agttggtgtc gtcaatcagt tttctcaagt gaaagcaatc ttgatttgaa atagcgaaaa
    14701 agagtcttcc ccagatttga aattaaaaaa agcttatgcg gatcaaagcg atggaagaaa
    14761 tgtgattaag gcgaatcttc aaaatccaaa tgcttcttat atgttcggct tgactacatc
    14821 ggttgaaatc tacaaaaaag aaacaaaaga gccttacctt tcaataaaaa aagaaaaaat
    14881 agatgtagca cccaatacaa attttgattt acttgtccca ttagaaggaa aaaaattagc
    14941 tccgggacat tatcaagcgg ttgtgaaggg tgcttggaaa gaaaaacgct ggaatctcca
    15001 taccgatttt gaaataactg atgatgcagc ggaccaattg aatgaaaagg atattgattt
    15061 aaaaaatcag ccagaaacat ttgactggcg atggctgata cttgttggag tcattcttat
    15121 tggcttattt gctggattat ttattttaaa agaaagaagg aaaaagaaat gaaaaaaaca
    15181 attgcattaa gtaccgttct agctggaagt ctattattat gtacacaaag tgtttttgct
    15241 gagaataaga gctcagccac tacagaagga acgatttctg tgaccaaacc agatgctgca
    15301 aatcctgaag acgttgttcc agttcctcct gttgatccaa atgatcctga tactcctttt
    15361 acacctgacg aaccaaatca gggaacatcc ggattactga cgattgacca agctccagat
    15421 cttgattttg gaacaatcaa gttaggtaca gcaaaaacag tatatgctgc attacaaagc
    15481 ggaacggaca gtactggagc acaaacacaa gtacagaatt tcgtacaagt aacggataaa
    15541 agcggaaatt atgccggttg gaaattatca gtcaaacgaa cagaatttat tggtacaaca
    15601 tctgatacga ataaactaac tggttcaaag gtgaaattca aaaacgccgt gattcgaaat
    15661 agcgcaacta atagcagtgg aatgccatct accgtttctg gaactggcgc tgaaggaatc
    15721 gaaatcccag cagatcaggc agtagatcta gtaactgcag gagcaaatga agggattggc
    15781 acatggattt attcattagg agagaatgtg gaacaaggga gacaatcagt ggaattattt
    15841 gttcctgtat ccaattatgc tgtagacaac tacgtttcaa cattcacatg gtcgttaaca
    15901 gatgctccta cagaagagga agcgtaaaat catatcaacg atttcaggga gtggagccga
    15961 ttcaaggccc actccctgaa atctgctatg cttacgtctc agctcttatt catcaactcg
    16021 aagcagtttt ttggaagtca tggaaacgtt cctttgtctt ttcaaagatc tttcgaccaa
    16081 cgagtaagga gtcaccgttg aaaaattcta acacataatg agcagaatca attttctgca
    16141 attgcgataa gttgatacag taagatttta aaagtaaaaa gtaagcatac ttttctaact
    16201 ctgtcttcaa ttgttttaaa gaagtattca ctaatttatc ttctgtagtt gttttgacca
    16261 taacttggtt tttcaaacct ttgatacttt caatatataa aatttcccga ggatacagga
    16321 cgagacttcc acctttttga tcaataaaga ttttatcgac atttacgatc tgctgttctt
    16381 tccaattgat ctgttctagc acttgacgaa tttgttcaga taaattcctg tcttttctga
    16441 tatatccaat cggaaggatt ttccgattga tgatctctac tgcaaaattc gtataagcgg
    16501 tgaaaaaaac aatattacta tgtgcatttt ttttacgtat ttgttcggcg acatcaatac
    16561 ctgtgagtga ttgcttcaag tcgatatcga taaagaaaac atcgtgatta tcaatagtgc
    16621 ccaactggat caactgattg atttgagaga tagaagtgag ctgcacgata ttctcgatat
    16681 gatgattgac caattctctg atgatccatt cgctgtgaaa ctggttatct tctatgataa
    16741 aatacttttt catatgcagg ctcctattaa aaaaatatgt gtacattttg aaacaaaaca
    16801 atggaggaat aagatggtcg caacagtgac actttatttg acctttactt tgattttaga
    16861 tttaggtatc tatttattct tttcgaaaca actgctagta aaaaacagct ttggttgtat
    16921 agctttgatg gtggtgacac ttttacttcc tatccctaaa gtgagctttc cttacctagt
    16981 tggcttatta ttgatgtttc ttttttattt tactacgaaa aacctgttta tcagctgttt
    17041 gatcagtaat cttttttttg ctatcactgg actcagcttt tttctggttt ttgatctgat
    17101 ggattattta ggaaagagac atcttggaac agcaagtttt tttgcactac ttatcgcatt
    17161 tattttctat ctattagtaa aaagtataga caaacagacg agatttctca acttgctttt
    17221 tacttatcca actgaacact attttggagg tatttgtatt ttattaagct ggggcgtgat
    17281 ttacttggta ttgggaagtg ttcacttccg ctcatctaat tatctgcttc tatctctttt
    17341 gtcgatagtc atcagttttt tttctttgtt tttttccatt atgttgatca gtttccaagt
    17401 aaaagaaaga caacatcagg aatcgcttca actgtatcaa ataaatgaag aatattacca
    17461 tcatctagag gaattcaaac atgattataa aagtctactg ttttcattaa aaattactct
    17521 tcaggaaaat aaaaaagaaa cgttggaatt tttagaaata ttagaagatc attctgcagc
    17581 tatttttgaa aaaccgcaaa tacaacagct aacaaatatt gtatccccag ctgtcagggg
    17641 tgtcttttta aaatttctag aaaaagcaga atcggagaaa atcccgatcc aagtagttat
    17701 tcctgtcgaa gtgaaaacaa tcccgattga tctggttgtt tttattcgct gtttatctat
    17761 ttttatttct aacgcattcg atcatcgtgt agccgatcag agtccgatta cgatcaatct
    17821 gctggaagaa aaagagggat cacgttttag tatagcgaac aaggcaaatc caacgaattt
    17881 gacgctatcg gagatggtca aaagaggaaa aacctctaaa aaaaatggcg ggttaggtct
    17941 atacagtgcg aaaaaaatgt tggatgttta cgaaaatgca gaactaaaaa tcgaattttc
    18001 aaaacagaat cactattttt ttgtagaaat gtatctggca tctagaaaga gcaaatcagc
    18061 ctactgactt actgtcaaag gataagtttg aggaaagtct acacggagat agagacaaca
    18121 acacagcata ttcaaaagaa acagaagcgt gatgaccgtt gtcacccttc tgtttctttt
    18181 gctatgtagt taaacaaagt agttgacact tccgctactt agtattagta cgaaagtata
    18241 cttcatgata ctaagtagat aagaagtgaa aagcggaacc cacgatcgtc aaaatgctaa
    18301 ctgctttgct tacaccagat aaagggaaga gcaatatcgc ttattttagg cgtggagaag
    18361 caagcaaatc cctacgatca caaaaaaaat gctgacccag cagttaagag agttagaaca
    18421 agatgaaagt atccatcgaa aagcctataa ccaagtcccg cctaaagtag agtactcgct
    18481 caccgaagaa ggaaaatcat taagagagat tctagtagcg ttgtctgttt gggaagaaga
    18541 acgtatcgaa aaacaaaaag cctcaaggga agaagacacg ctactgcata cacatggtgg
    18601 ctacctaaat tattgatttt ctatgacatt tattttaaat aaaagaaaac aggagttttt
    18661 ttaaaagcat catctatttt ttatgtaaaa aaatggtatg attaatcaag taagaagtga
    18721 ttggaagaag gcaagcaaat gaatagtatg gaaaaagtca accagtttag agatgaacgt
    18781 aattggcgac aatttcataa cgaaaaagat ttggctatct ctatttcttt ggaagcatct
    18841 gaattgttag aactttttca atggaaaacg ccggaagaag taaaagaaac acaattagaa
    18901 cgaatcaaag aagaactggc agatgtattg atctattcat acatgatggc agacaacctt
    18961 cacctggatc tagatgagat cattgaagaa aaattgatca aaaataattt gaaatatcct
    19021 gtagaaaaaa gcgcaggtgt acgaaaaaaa tacacagagt tgtagaaatg tgtcattcgg
    19081 ctgttggaaa gagcaaaaga aaaacagtaa aatgagtctg gagggagaga aaatgaaaaa
    19141 aaaaggaaaa gcaaaaagat ggctagtcaa tatcctcttg ttcctcttgt tattagtagg
    19201 actagctttg attttcaatg aacagatcaa ggattatttt gtcagagaga caggagataa
    19261 atatgctata gcgaatgtaa caaaagaaga tctgaaaaag aataacgata aagatgtcag
    19321 ttttgacttt gatgcggtag aaccaatgac gacagaaggg gtcatgaggt cgcaaatgag
    19381 aggaacagat ctaccagtga ttgctagtat cgcagtccct tcagtttcaa tcaatttacc
    19441 ggttttcaaa ggcttggata atacgtcact actttacgga gcaggaacat tatctccaga
    19501 tcaagaaatg ggaaaaggaa attatgcctt agcaagccat cgtgcaacga accctgaatt
    19561 gctgtttaca ccgctagaaa atttagagat gggtgccaag atctatttga cagatttaga
    19621 aaatgtgtat acatacaaaa cattttttaa agaaaaagtt gctcctacag atacccaatt
    19681 actgaatgaa gtcgagggaa aagaaattgt cacactaatt acttgtggcg acatggacgc
    19741 cgtcacgcga ctagttgtac aaggagaact ggaaagtgtt acatcgataa aagatgcgac
    19801 agatgatatg agatctgctt tcaatctaga gacaaaaaca ttttaggata ctgggacata
    19861 agtcgttaca gattaaaaac agccaaagga tatgaatcga taaggaaaaa ccaatttgct
    19921 gaaaaaaaga gaaacgcaaa gaggtgcatc tctgatttgg ttgttttcta gatagacttg
    19981 tgttccagtt tttttatttt ttatatgaaa aggcttgcat aaataaaaat tcgatggtat
    20041 gatgattttg aataagaaat ggattgttac tgtacggcag gcaaaaccag aattcagcta
    20101 agaaacagta ggcgaattct aggttttttt atttttcttg agagggcgaa gcaaaatgaa
    20161 aattgataaa attttgaata acaatgtcgt catttcgaaa aatggattcg gggaagaagt
    20221 cgtttgtatg ggaaaaggac tagcttttca aaaaaagatt ggggacgaga tttcgccaga
    20281 agcggtccaa aaagaatttg ttttaagaga ttcttttgct aaaaaccaat tccagcagct
    20341 gatggctgat attccggcag aggagatcga atgggtcaaa caagtagtcg aactggctga
    20401 agataaacta aaagttgaat tttcgcctaa tatttatctg acattgaccg atcatcttca
    20461 ttatgcgatc acacgagcaa aagaaaatat ccaactgcct aatccattat tatttgaaac
    20521 aaaaaaattt tatccgaaag aatatcaagc agcgaaagag gctcttttgt tcgtgaaaca
    20581 aaagacgggt gtggaacttt ctgaggatga agccggtttt attgcctttc attttgtgaa
    20641 tagccaacaa ggaaacgaag acatgcaagt gacgatgacc gctaccacaa tggtaagaga
    20701 tatactgagt atcatcagta aattcttcgg catcgtgttc gatgaagaat ctttgaatta
    20761 tcagcgtatc attactcatc tgcagttttt tgcccagcgt tatctcaaag gggaaggatc
    20821 tgacgagcaa gatgaatttc tctacgcatt agttcaagga aagtatccaa aagcgtttca
    20881 ttgtgtcgaa cggatcaatg agtatcttcg acaaacgcaa aaacaggaaa tggggatcgc
    20941 agagcagatt tatttaacga ttcatattca aagagtagta agtgagaaaa aaagcatcca
    21001 atcataagga ttgttactgt atggcaggca aaacctggat tgaaaaaata ggttcacgaa
    21061 tgtttgtgac tgtttttttc aatctgggtt tttatttttt aaataaaaaa gaaagaagga
    21121 aaaaacaatg gataatcaag cagttggtcg tcgtgtatgg gaagcggtag gaggacaaaa
    21181 gaatgtcaaa agtttagtac attgtgctac gcggttgcga ttcagattaa aagatgaatc
    21241 tttagcagat acgcaaaagt taaaagaaga cccggacgtg atccaagtcg tacaaagtgg
    21301 cggtcagtat caagtagtga ttggcagtaa tgtagcagat gtttatcaat cgatcgtgga
    21361 tgaggaaggt cttgcagttt cggaggatgg aaaagaagag tccaaaaata ttttgaatcg
    21421 tctgatcgat attatctcta gtatatttac accttttcta ggggcgatgg cagccgccgg
    21481 ggtattgaaa ggattcttat cattggcaac ggtgatgggc tggctgacag ctgatagcgg
    21541 tacttatcaa atattgtttg ctgcagcaga tggtgtcttt accttcttac ctgtcatgct
    21601 tgcttttaca gcagctaaaa agttcaaagc caaccagttt ttagcagtag caattgctat
    21661 ggcacttgtg tatccagcta tcacagcagt tgctggtgcg ggagaagcta tcagtttctt
    21721 tgggatccca gtgattcttt caccatctgg ttatacttct tctgtcatac caatcattct
    21781 agccgtatgg gttcaaagta aattagagcc attcgtaaaa aaagtgattc cgcaatttct
    21841 gcaaatgatt ttagttccgc ttgtcgtttt agtagtgatg gttccattaa catttcttgc
    21901 tttaggacca atcgggacgg ttgccggtaa tgctttaggc ggtctcttca actcgattta
    21961 tggatttagt ccaatcgtag caggtctaat catgggaagt ttatggcaag tattcgtgat
    22021 gttcggcatg cattggggat tcgtaccgat catgtttttg aatatcgaac aatatgggtt
    22081 tgatgtattg atgccaatgc ttcttccagc catcttggca caaggtgggg ctgctttagc
    22141 tgtagcattg cgaacaaaag atacgaaact tcgagcatta gggatttctt ctacagtgac
    22201 ttcgttattt ggaatcactg aaccaactgt gtatggtgtg acattacctt tgaagaaacc
    22261 attcattgcc gcttgtattt ctggaggtat tggtggagct attatcggat tttccggagt
    22321 aaaagctttc tccagcagtt tggtcagctt attgacgatt ccgactttca ttaatacggt
    22381 ggatggtgta gagtcaaatg tgacagtagc agtaatagct acaggaattg cttttgttct
    22441 tgcattcgta ggtacattga ttttaggatt tgacgaacaa acacaagaca atcaattaga
    22501 aaacaaacat gcgaatgcag gaaaaccgat tacttcagca cggcatacct taaaaagtcc
    22561 tttaacgggt aaagtactgc cattgagtga agtaccagac caagtttttt cttctggcgt
    22621 gatgggaaaa ggtatcgcta ttgatccaga agtaggagaa ctagttgcac ctgcagacgg
    22681 agagatcacg actatttttc caactggaca tgctgtaggg atcacaacga ctgacggagc
    22741 ggagattttg attcatatcg ggatggatac agtagaatta aatgggaatg gctttgagat
    22801 cttagtcaaa caaggggatc tcgtcaaagc aggagattta ttgattcgtt ttgacatcga
    22861 agcaattaga gcagctggct atagcgtaat cactccagtt gtcattacga atactgatgc
    22921 atttgccgat atacttgaat tagaccaaaa agagattatt gcaaacgaag atgttctggc
    22981 aattgtaaaa taaggaggaa atcaggatgc aatcacgttt ttcagaaaaa tttttatggg
    23041 gtggagcaac tgctgccaaa tcaattagaa ggggcttatg atcgagacgg aaaaggacta
    23101 tccgttgcag atgctatgcc tggaggtaag cagcgctttg cgattatcgg cagtgaagaa
    23161 tttgactgga cgattgatca agaaaaatat atttacccga atcatcgagg aatcgatcat
    23221 tacgatcgtt tcaaagaaga tcttgccttg tttgcggaaa tgggattcaa atgttatcgt
    23281 ttttcgattg cctggacacg aatttttcca aatggagacg aaggtactcc taacgaagcg
    23341 ggattagaat tttatgatca gctgattgat gagtgcttaa aatatgatat tgaacctgtg
    23401 atcacgattt ctcattacga aatgcctctt catttggcaa aagaatacgg tggctggaaa
    23461 aatcgaaaac tgatcgactt ctatgaacga tttgctcaga cagtattaga gagatacagc
    23521 agtaaagtca aatattggat gactttcaat gaaatcaatt cggcctttca ctttccagct
    23581 ttaagtcaag gattagtaaa aagcaatgga gcaggagagt accaaaatat tttccaagca
    23641 tggcataatc aattcgttgc aagtagcaaa gccgtaaaaa tcggccacga attgcgttcg
    23701 gatatccaaa tcggctgcat gattatctat gcaacaactt acagcatcga tgccaatcca
    23761 gtcaatcagg cagcaacaat gatccaaaac caagaattca attttttctg cacggatgtc
    23821 caagtacgtg gagaataccc tgcatacaca gcgcgtaccc ataaaaaata tggagtcgat
    23881 ccggaaaaat tagagcaaac ggaaaaagac ttccagttgc taaaagaata tccagtggac
    23941 tacatcggat ttagttatta tatgtctacg gcaatcaatg agactgatcc acaagcagca
    24001 acttcagaag ggaacttgct gggaggagta aaaaatccgt ttcttgaggc aagtgaatgg
    24061 ggctggcaga tcgatccaga agggctgaga atcgcattga atgaattata caatcgttac
    24121 cagaaaccat tattcattgt tgaaaacggt cttggagcca ttgatcatat ggaagaggat
    24181 ggcacgatta ttgatgatta tcggatcgat tatttacgtc gtcatatcga agcgatggct
    24241 aatgcagtag cagacggtgt agatttgatg ggctatacgc cttggggatg tatcgatctt
    24301 gtcagtgcat caacaggaga aatgagtaaa cgttacggct ttatctatgt ggatctagac
    24361 gatgaaggaa atgggacact gaatcgatca aagaaaaaat cttttgattg gtataaagaa
    24421 gtaattgcct caaatggcgt aaacttgtaa ttaaagcagc tgaatgaaaa agaacacact
    24481 tctcacatgt gatttgtgtt ctttttttat tttttatgtg ctattctttt agtaatagta
    24541 aagcaaaggg tgacgttatg aaaaggacac gtgtactata cttggaagtc gcagataaaa
    24601 ttaaagaaga tattttctca ggaaaatatc cagtcggttc gatgttgccg acagaaagtg
    24661 aattagaaga actgtttcaa gtcagcaaaa tcactgtccg aaaggcaatc gaactgctgg
    24721 catcagatga atatgtcgca aagaaaagtg gtcgtgggac gacggttcta agcaatcgtc
    24781 cttacaatcg attatccaaa gcggcaagct ttacgcaaat cttggagaac tcccgatatg
    24841 aagtccggaa agaaacgctg gatttgcaat tggtccatat aaagaaggaa gatcctttgt
    24901 tttccttttt cggtgagcaa gctgtttgtt ttaagcgctt atattatttg aacaatcagc
    24961 cttatattta ttttgttcat tatcttccac aagaaatgat gcaattcagc agagaagaat
    25021 ttggcaaaga atctttgtat cgtttgctga cgaaaaaagg atatactatt gactcatttt
    25081 ctgatgattt ctcggcagta tttttaacag aagaagaaca aggaattctg aaaacagacg
    25141 cacagatcgg catcaaacgg atacggaaat cgtctacgca aattggggaa atgattgaat
    25201 attcggaggc ggtctaccat acggagctgc atccttacca tattgattat gaaacataaa
    25261 taaaaaaaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag ccaaacaaaa
    25321 atagcttctc atatttttcg tttggttagg cttattaccg cagatacacg gctgtttctg
    25381 gattaccatt tattctggat aagggggtgt ggcacgattt tcagaaggaa tctatgaacc
    25441 aatcactgta ttgattttag cttcgatggt aaccccattc ggcttgacga tcgcttattt
    25501 cttagggaaa atcattcgta aaaatatact caatcgtcaa gaaatcgata cattgaagac
    25561 cgcttttcct atggggatct gtcagatcac agagggatgt ttccctatcg tattgaacga
    25621 cttggcacgc aatgtcatag caacgggagt cggtgggaca gtaggtggcg gattaagcat
    25681 gttttggggc gcagatagcc gtattccggc atcgggaatg tttgcagtcg ctacgatgac
    25741 cagaccatgg gcattcattg gtgcattgtt agctggttct tttgtcacag gaataaccat
    25801 tttattattg aaaaaaccag tagatccgaa tgcagaaatc atccaagaag aaaaagagga
    25861 agaagacata tcttgggatg atttgacaat tagttaggag aggcagaatg gattacaaga
    25921 aaaaagtaca atagatgtac cttgagacag gaatggtatg aaagaatcag agttagaaag
    25981 aatcgaatat acagattttg gtttaggagc gatcgaaact gtccagagca tcggcaccct
    26041 ccttgaaatg acgatgaagg aaaaatgaga acctttcgtt gtcgaaaggg tctggtatac
    26101 cttatgtcga aggaaaggaa acaaaaaaac tttcctgtac tattccaaaa aggaaagaaa
    26161 gttattatac agtaaaaaaa gaaattattc ttcatgctag agatcagtac acgttcgagc
    26221 ccaatatcaa acactagttc caggcaggcg caacaggtgc ggttatcact gaattttctt
    26281 ccagcagtga cgattcaagt gatattttta caaatccagc tatttatcgt taacaaaaag
    26341 atttttagga gaagctgatg aacaaaaatg atcaaatttt gatcgtttag ttcgtcggct
    26401 tttttattta gagaaacatc atttatcagt gaaacataac acgagaacaa aatgattttt
    26461 tcgttaataa aagaaacaag tatttgttgt ttaaaataat acacgttatt ggcatatagg
    26521 acgaaatccc agtatcttct ataagtaagt catttcatat cgatgaaaac acgttattta
    26581 tttttgtttt atctataaaa tggctgaaaa atgatttttt tctaaatact atatatagtg
    26641 gcgtctatcc tatataccac taaaaaacta caaaatctag tcttttattt taaaaaaaca
    26701 cgtgtagaat gaagatagac ttttgagtta gggagtgttt ttttatgaaa cttactgttt
    26761 tttgcggttc ccgttttggt aataaagaaa gttacaaatt tattgcgcaa acgttaggaa
    26821 aatacatggc acaagaagaa atcgaactgg tttacggtgg ttcatactcc gggattatgg
    26881 gagtgattag taaaacagta ctggaaaata acggaaaagt cacaggtatc tatccgaatg
    26941 ggctttttga aactgaattg cctggaaaag atgttactac atttattcct acagaaacga
    27001 tcgatgaaag aaaggtatta ttatttgaaa agggagatgc tgtcctcgtt ttccctggag
    27061 gtctaggtac attggaggaa ttctcacaac ttctttcttg gatagcaatc ggtctgacgc
    27121 ccgacaagcc aatcggaata ttgaatattg gcggttatta cagcggcttg caacaattat
    27181 tagaaacttt tgcaaaagag gattttatgg ataaaaaatg gttggatcaa gtaatctttt
    27241 ccaacaatcc attggaactg gtcaatgaat tacgagcagt atctattaaa aatcagttat
    27301 tattgaagga ggcaaaataa tgagtcaaat atatttagca ggaccatttt tttcagagga
    27361 gcaaatcgat cgagtaagtc gtatcgaaaa agcgttggag gaaaacaaaa cagtcacaag
    27421 tttctactct ccacgtcatc accaagaaag caattatgaa ctattcagcg ctggatgggc
    27481 gcaggaagta tacgaaaaag atatggaaga actgacaaat gcagaatttg ttgttgctat
    27541 ccttgacttt gagcatcaaa caatcgatcc aggaacggct tacgaattag gtgtggcaac
    27601 tatgttgaaa aaaccgatga tcattgtaca agaagagacg gtacctacga acttgatgat
    27661 tacacaaagt ctccatacgt atttaaaatc agaccaagct gtacgtgaat atgattttga
    27721 aacattgcct gttgaaactt acgtaggcga gtacttatag aagaggaagg gttgtatgtc
    27781 agtgattttg atttcaggaa cgattggtgc aggtaagagc agtttgacgg atatgcttgc
    27841 aaaagagatt gattctaaac cattttatga aaatgtcgaa gataacgaag tattgccgct
    27901 cttttattca aatcctgagc agtacgcttt tttattgcaa atcttctttt tgaacaagcg
    27961 atttttagca atgaaaaatg ctttagtgaa cgatgacaat gtgctagacc gatccatcta
    28021 tgaagatagc ctgttgttcc atctaaacgc tgatttaggt cgggtaacgg atatcgaagt
    28081 acagcagtac gatagcttat tagatacgat gctaaacgag ttagatgatg ttgctccaaa
    28141 aaaacggcct gatttgatgg tccatatcaa agtttctttg gacacgatgc tagaacgtat
    28201 caaaaaacgt gggcgtgact atgaacagct agaaagcgat gagacgttat atacctatta
    28261 tgaaacgttg aacacccgct ataatcaatg gtatgaagat tttgatgtat gtcctaaaat
    28321 acaagtggat ggggataaat tcgattttgt agaaaatcca gaagatgcaa aaaaggtgat
    28381 ccgtcagatc caagaaaaat tagctgaatt ggaaggaaag cctgctggaa aatattttac
    28441 ggataaccag catgcaaaag gtcaagaatt aggctaaaaa taaaaaacca aacgacggga
    28501 aagaaaaatt actacgtgat gtagaataag cctttctatc gtttggttat tttatattca
    28561 aatatttgaa ataaataaat ctttactctt tgatttgtaa actgaaatga tccactgttt
    28621 gttttactga tgaagcttga tcaaaggaag aagctgagtt tgaagttcat aaaaatcagc
    28681 catagtgagt tttgcttgga tttgtttatc ttcttctact ttttacataa ttttgaacag
    28741 gataagattc aaatgtttta aatgaatctt tgactagttt gtgacagttg tcgcatatgc
    28801 tcaagcttcc ttggtcatta aattctgtat ggatattttt gttgttatta catgtacaat
    28861 aatcaggact atcgatcatc catttcgtct cctttacaat catcttgctt ctttactata
    28921 gcagaaaaga aggggagaaa caaaagaaga tccaacagac ggcactatag actgtagatg
    28981 ttgccatcct catccttcaa acggtaatta ccgatttttg taaatatgac tgttgtttct
    29041 tttttatttt cttttcctga aataatatat tcgtcatctt tggtagtgat cttgatatct
    29101 gtcagcactg ttttttcttc agatgattca gaagaatcag ttccttgtgt ctgatacgtg
    29161 cgtgtccatt ccattttttt atctgtaaat gtcaactgtc ccgcggataa atccatatct
    29221 ttacgtactt ttagcgtata tacactgttg gtggtatctg ctttttccgc ttttaagaca
    29281 gaagaatcgg ttgtttcgtc cgaggattgg tcagagccgc caaacaaatc acagccagtc
    29341 aatgtcaaaa gtcccgcaga caaaataaat agtacgatta atttcttcaa aatacttccc
    29401 ccatttgata ggataaagaa attataccat tggaatgtaa aacttcaaac ggatggaaga
    29461 gaaaaacgca caaaacaccc acaaatagag taaattttag aataaaattt taaaaaggca
    29521 aaaaatccgc cactttcaga aagcgtttta aaaaagtgtt gcaatctttt aaaaaaatgc
    29581 tatattaaaa atgaaccggt tcaaatactt gtttaagtat aattaatagt gagaggaagt
    29641 tgctctactg aagactttag ttatatggat attgatgaca agcaacacta tgcaaagaat
    29701 gcggtcatca taatcgaaca acaagaaaga gaaatcaaat gtaactggca ggagtgagaa
    29761 tgtatggcat cgatccgtga tgtggctaag atggctggtg tttcagtggg aacagtttcc
    29821 cgttacttga atggtcagca actgaaggaa aagaacatga aaaaaatagc agaagcgatt
    29881 tcagcgttgg actataaaga aaacatcatt gctaaagggt tgaagaataa tcgaagcttc
    29941 tctattggac ttttgataaa cgggatttcc agccgttttg ggtcggaagt tgtctcaggg
    30001 atcgaaagag tagctgaaga aaatgggtat agcttattat taagcgggtt tgccgatcag
    30061 ccagaaaaaa ttgaacaaaa aattgaatac ctgatgaagc atgtgatcga tgggctgatt
    30121 gtcttcttat ccgaagaaga gtggtctgga tttgaaatgc tcacaaaaat gagtattcca
    30181 gttatcgcat tgaattgtcc agaaggacca gcaggtattg atacaatatt agtgaatgat
    30241 cgagaaagtg tagcaaaagt gatcgctcat catggtgaac aaggacataa aaaaatcgga
    30301 tttattgctg ccactcaaac agactacgta gcaagagaac gtttagcagg agccaaagaa
    30361 gcggtccaaa atgatccatc gatccaatta gaggtcttta caggagacta ttctagaaaa
    30421 agcggctatt acggggcaaa agcattatta gaaaaagggg caactgctat ttttgtcagc
    30481 aattacaata tgtcgattgg tgccatcgaa tgtttcaatc aagaaggtgt cagaatcggg
    30541 aaggatattg cgttcagtca ctatgattat ttagataaaa acgatctgag tatccttccg
    30601 aaaatcacga tcacgccacc aacagataaa atcggggaac gagcagcaga acagctgtta
    30661 aaacgaatca aaaaagaaga ggaagaaaat gaaaaagtga ttgtgatgga aaatatcata
    30721 catggaatca actaaaacga actaaaaaag aaaaagaggt gctttgatga aaaaatatca
    30781 atttccagca aatttttggt ggggttctgc agcaagtggc ccacaaacag aaggaaggat
    30841 ttcaggcgat ggaaaaggag agaacatttt tgattattgg tacaaaaaag aaccgcaaaa
    30901 gttctttgat caagtaggac ctgaaaaaac ctcgcaagtt tatacgaagt atcaagagga
    30961 tgtacagttg atgaaacaga ctgggcataa ttcatttcga acatctatcc aatggagccg
    31021 tttgatacct gatgaatctg gaacaatcaa cgaagaagcc gttcgtttct atcatcgtta
    31081 ttttgatgag ttgattgaaa acggtatcga accctttgtg aatctgtatc atttcgatat
    31141 gccgatgtat ctgcaagaaa aaggcggatg gttgaaccga gaaacggttg aagcctatga
    31201 aaaatatgcc aaaagttgtt ttgaattgtt tggttcgaaa gtaaaaaaat ggtttactca
    31261 taatgaacca atcgttccag tcgaagcagg ttatttatat cgatggcatt atccagaaga
    31321 gtcagatatg aaaaaagcga tccaagttgg ctatcatgaa gcgttggctt cagcgttagc
    31381 catcaaatct taccatgaaa tggagcaagg tggtgttatt ggtatcatct tgaatctgac
    31441 accaagctat ccaagagacg aatcaaaccc tgaagatgtt aaagcagcgc aaatagcaga
    31501 tgcgttcttc aatcgttcat tccttgatcc agctgtaaaa ggcgagtttc caagtgaatt
    31561 gattgagata gtaaaagaac ttgatctcat gcctgtgatc catgcagagg atttgacggt
    31621 tatcaaagaa aatacagtag atcttttagg tatcaattat taccagccac gccgggtaaa
    31681 agcaaaagaa acaccaatcg atactaaaaa cggtccaatg cctgaggatt atttcgacaa
    31741 ttatgaaatg cctggtcgta aaatgaatcc ttatcgcgga tgggagatct atgaacaagg
    31801 aatctatgat attttgatca atgtacgaga tcattatgga aatatcgact gctttatttc
    31861 tgaaaacggt atgggtgtag aaaacgaagc acgatttatc aaagaagatg gcatgatcca
    31921 cgatgattat cggatcgaat ttgtccaatc tcatctgaaa tatgtccatc aagccattca
    31981 agaaggtgcc aattgtttag gctatcatat gtggacatgt atggataact ggtcgtggac
    32041 caatgcatat aagaatcgat atggctttat ttcagtagac ttagcacaag atggaaaaag
    32101 aacaatcaaa aaatcgggtc attggttcaa agacgtatca gataaaaacg gatttgaggc
    32161 ttaagaaaga atagttgaga gcaaatgcgt gaaattctcc attcttacca ttttgtgaaa
    32221 aagaagttca attatttacg atctttttca acttttttgt ctttctattt cattttttgt
    32281 atataataga atagtcaaaa ttaaatagga gtggaggaaa gcatgaggaa aaaaagaccg
    32341 tttcttctct tttggttatt cggttttttc ttactgctta ttttatccgc agcattttca
    32401 ggaaaaatga cagcagatct tgctcagaaa gaaacagaaa aaatcgtagc aagcaaagaa
    32461 tcagtagata atttcaaaaa agaattctca acttattggt gggcggagta taaaataact
    32521 gccaattacg aaaaaggcga attggacatt tatttgccaa aaggaaaaga agaaaatcaa
    32581 tttattaata tcgatgacgt acttagtgcg atttctctca gacaatatca atttaaagac
    32641 aagcatttga ccgttttgct tttgactcat gatggcgaca aagtagcccg tttgaaaaat
    32701 ggaaaagtga tcaccgctta ttctgaaaaa gtagacaaaa aagtggtcgc cgatcgttta
    32761 gcggaatgga aaaagacaaa tgaacaagtc aagaaaacaa ctgtctcttc tcaaaaaaac
    32821 gagtcgacag gaacaagtcc ttcagattct ggcagtggac aaacgataac gggaagtgaa
    32881 gaaaatcaag cctcaattat tacgaatgag tcatcacaag gacaatatca atcctatgga
    32941 caatgaaaaa cagaggttgt gaaatcagcg ttttgacctg agtattagag aaacaaacgg
    33001 caaaatagtt cctcatattt tgccgttttt tgagctaata ccgaaggtca gctgattgaa
    33061 caccgtgtat caagaggtct tgagcctgcc gtttaactct gagccacaag gagcaatttc
    33121 aaaaaatagc tccttatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
    33181 gaacaccgtt taccaagagg ttgtaaaatt agcgtcctga cataatctaa taccgagggt
    33241 cagctgattg aacaccgtgt atcaagaggt cttgagcctg ccgtttaact ctgagccaca
    33301 aggaacaatt tcaaaaaaca gcttctcatg ttttataaat attgtgactt gtggccgagg
    33361 agttggcagg agaacaccgt ttaaggaaga agttgtgaaa aagctgtcct gcatcaagaa
    33421 ataaggagct gcgacaagaa ttttgtcaca gcctcttttt ttgtacgaat accgagaaac
    33481 tggcttttga ataccgtgga gtgaagcggt caccacaaga cttttatctc ttcattagtt
    33541 taatcgctta catcgcagcg gttttatgct gtgaaaagtg gacggaataa tctttagaaa
    33601 gaataaaaaa atcttaagat aacaactttt ttaggaaatt ttcatttttc aggagcaaaa
    33661 taagaaaaaa tgtgaataga gagaagtgat atacgtgaca caacttaaaa aattgtgcca
    33721 aaatcgttta gcaatcgttt tgacagctgt tttctttttc tggctgaaga cgatcattgc
    33781 ctactatgca gatttttcat taggtgtgga aggtacgatc cagtacttta tcttgtggat
    33841 caacccaatt gcgactacct tattgttttt tggcctttct ttgtatatta aaaagccaaa
    33901 gccagcgtta gctattcttt tgatcattga tattttaaat acgcttttgt tgtacttgaa
    33961 tattattttt tatcgggaat tcacagattt cattacagtc aaatctgtat taggcttttc
    34021 gaaagtgtcg caaggattgt caggaagctc cttttctttg atgaagcccc atgatgtcat
    34081 ctactggctg gatatcgctg tgttcattgg gctactggtt tggttgaagg tcaagaaaat
    34141 acctatcaaa agcaaccctg tcggcaaacc gatggctatc gctgttacct gtctttcagg
    34201 gcttattttt tcaggaaatc tagcgctgag cgaagccaac cgtccacaat tgctgcagcg
    34261 aacatttgac cgttcatata ttgtcaaata tctaggaatc gatgcgtata ctatttatga
    34321 cggaatcaaa acaggaatga ctagcagtgt tcgcgcgcat gcaagcagta atgggattga
    34381 tgaagtttta gattatacaa agaaacatta tgcagagcca aatcccgaaa catttgggat
    34441 cgctaaaggg aaaaatgtga tcgttctcca tttagaaagt ttccagcagt ttttgattaa
    34501 tatgaaggta gacggacaag aagtcacacc gtttttaaat agtattttcc agaatcaagc
    34561 aacgatcagc tttgacaatt tctttcatga agtaggacag ggaaagacaa gtgatgctga
    34621 aaatatgttg gaaactggca catttggttt acctcaaggc tcattattca cagaacttgg
    34681 gtcagacaat gtctttcaag ccgcaccagc gatccttgga caaaagcaag gctatacaag
    34741 cgctgttttc catgggaatg tagctagttt ttggaataga gatcatgtat ataaaaactt
    34801 aggttatgac aatttctttg atcgatctta ttttgatgaa tccgacgaaa cgttaggtta
    34861 tgggatatta gataaagatt tattcagaga atctgcccaa tatcttgagc atctacaaca
    34921 gccgttttac acgaaatttc tttccgtaac caaccacacg ccttattata cggatgacaa
    34981 gaatttcgac tttccatctt tgaatactgg caatagcaca gtagataatt atgttcgaac
    35041 ggcacattat ctggatcaat cattggaaca attttttacg tatttgaaaa aatctggtat
    35101 ttaccaaaat tcaatctttg tgatttacgg ggatcatttt gggatctcga atacagataa
    35161 taaagattta gcaagcgctc ttggtaaaga tccagataca tgggatgaat ttgacaacgc
    35221 acagatgcaa cgagtgccat tgatgatcca tatgcctggt tatactaaag ggacagtcaa
    35281 tcatgaatac ggtggagaaa tcgatgtttt gccgacatta ctacatctat tgggaatcga
    35341 tgataaagat tatcttcatt ttggtacaga cttattatct tctcagcatg atcaagtagt
    35401 tgcttttcga aatgggaact ttgtgacacc caaatacacc gtcctaggag gaaaagctta
    35461 caataatcag acaggagaaa tcatcgatac gaaagcagca ggaatcgatg aggaaatctc
    35521 caaagatcag gaaaaagtca agactgcgtt aagtctttca gataagctga atcaggaaaa
    35581 tctcttgcgg ttttatgtgc caaacggatt tgagacgatt gatccgaaaa aatatgacta
    35641 taaaaatgaa gcggaacgta atgaagcaat cgaaaagcaa aaaggcgaac attcgacaag
    35701 tgtcttttca aaaaatggta ataaaacaac aaccaactta tatccagtaa caagaacaga
    35761 cgttgaacag caaaaaaata atcaataaac aatcaaacca accttcagta gttagacaac
    35821 attctaatgc ttgaaggttg gtatttttta tgaaaaaaac atttttttat ttttttcgtg
    35881 tatttttcct ttttaacgta tctatttatt gaatgtggaa aaatttaaat taaaaaggag
    35941 aactattatg tatgatggtg aattgatcaa gaaacttcga gtcaacaaga agttgacaca
    36001 gtcccaatta gcggaaggaa tctgttttaa gacttctcta gtcgggattg agacaaattc
    36061 agtgaaaaaa atgtcatttg taacattgag atcattttta gagcgaatga acatgacact
    36121 tgcagaatat gaatggatgc gaaaccagct ggatgaacca ataaagttaa agaaaaaaag
    36181 acgaatgcta gatcgggtac aagaagacag ctttgatcct tataaagaga tcagcaataa
    36241 ccgtaggcat tttaagaaaa cagaagatct gtattatttg gttttgaatc ttcacatggt
    36301 cgaaaaaaca gaagtatctg agacagaatt tgaattttta cgttatgaat gccgaatcat
    36361 tgaagaatac ttgatgcaga taccagacta tggtcaattt gaactatcta ttctagctga
    36421 ttttccttgg atattcagtg atcggttcat tgagaacaat tatttgaaaa tcaaaaaaag
    36481 aatgcgacga ttagcacagt tgaacaatca tgagcagtat ctctttactt ttttaatgaa
    36541 cctgacttcg ttttatatag aaaatggtcg gttgaaaaag gcacgtagtg tgaatgaaga
    36601 tatgaaacgg agcttagcac ataaagaaag aagtacagtg atctatgaaa cattgatggc
    36661 agattattat caaagattga tttctgctgc tttaggggaa gaaaagacga aagactacca
    36721 agaattcttc acagtgctcg aatatatgtt aggaaagaat caacggaggt ccttagagaa
    36781 aaaactgctg gccattgctg aacccagcaa ctagaccagc agtttagaat ttgtatcact
    36841 tcgccgcttt ttcagacggt ttgtttatat cgaatggcgg gaaaaagcgg gcgatctctt
    36901 ttaccgcagc atcacgggaa tcagaagcgt gaatgatgtt ttcagtcccc ggcagcgcgt
    36961 agtctcctcg gatcgtacct ggaacagcat cggcagcttt tgttgctcca accatcttac
    37021 ggacgatatc gatcacttcc tcgccgacca gcacaagata aacgacagga ccggaagtca
    37081 tgtaatcgat cagttcatca aaaaaactgc gctcagtcaa gtgctgataa tgttcttttg
    37141 ctaattcagg agtcatagtt tcaaacttca tttcagcaat ggccagtccc tttgcttcaa
    37201 aacgttggat gatgcttccg actaaatggc gtctcacgcc gtctggcttg ataataacaa
    37261 gtgttctttc cataacagca cctcgtttcc tactatattt ctattatagc gggaggtgga
    37321 aaatgagaca aataaaaccg cgtgagacag gaatataacg tgtgtatttt aaaaaaagta
    37381 tgaaaaacat ggaaaattga tttgacatcc tcttttttag ttgttatatt aacgaaggaa
    37441 aaccgaataa gatttgttag gtgaggctcc tatacaaaca taggctactg cccaaaaacg
    37501 tcgagagacg ccaatgggta gaacaggaat tgtcgagata aggcttttca taaggtagct
    37561 aaaagcaggt atctgctttt tacgttgtac agtgtcaaaa ctcaacgatg agatctgaga
    37621 agcttttttt aatatgcttt ttcaaacctt tcgctgagta tttggcgaga ggtttttttg
    37681 ttgccgcaaa tccccatcgg tgaaaaataa aaaggaggtg gtctaattgg aaaaataact
    37741 attcgaaaaa tgattggaag tcatgaatta aaattactta gtaaaggaga tgagaacaaa
    37801 tggatgacag tcattcgaaa aacaaggatc tagaggaaag gccagggcta ttatctatct
    37861 gttctcagcc cctttcagtt ttttctctat cgatccgatg aatagctaaa gaccaaaagc
    37921 agcgagaaca gctgtctttt ggacacaaag gagagattga gatggaaaaa aagaaagata
    37981 cacaaaaaat ctatgcagct tttgctggaa aaacgaaaca agaattattt gaactattcc
    38041 atgcatcagc aaatggattg acagaagaac aaatcgaacg ttcaagagaa aattacggtg
    38101 aaaatgcgat ttcatatggc aaaaaaacac cattcattgt agaaatattg aaagcataca
    38161 tcacgccatt tacattagtg ttgatcgcat taggaattat ttcattcatc acggaatatg
    38221 tattagcagc tcctggagat aaagatcttt tcggtgtagc cattatcttc actatggtta
    38281 tcgtcagcgg gacaatgacg ttgattcagt ccgtacgttc caatcaagcg gctgaaaaac
    38341 tgaaatcact tgtgaaagta acggcagctg taaaacgtaa tggaaagtac acagagattc
    38401 ctatggaaga aatcgtctgt ggagatcttg ttcggctttc agcaggggat atgataccag
    38461 cggatatgcg tttattgtcc gcaaaggatt tattcatttc ccaagcagca ttgacagggg
    38521 aaagctatcc agtagaaaag catgcgaaag tatgtgaaga cgatcagcaa agcgaaacaa
    38581 gttacgaaaa tcttgtcttt atgggaagta atgtcatcag tggaagtgcg gaaggaatca
    38641 ttgtttctgt tggaaatgaa acactatttg gtcatgtagc agaaacgcta tctgaaaaac
    38701 caattcaatc aagctttgaa ttaggcatcc ataaaacatc catgctactg atcaaattta
    38761 tgtcattgat ggctccagca gttatcgtca tcaatgggct tacaaaaggg gattggctag
    38821 aagcttttct ttttggacta tctgtagcag ttggtctaac acctgaaatg cttccaatga
    38881 tcgttacgac gaatttggtc aaaggagcaa gtgtgatggc caaaaaagga acagtgatca
    38941 aaaatttgaa tgccatccag aactttggtg caatcgatgt gttatgtacg gataaaacgg
    39001 ggacattgac acaagataaa atcattcttg aatatcatat ggattgtgaa ggaaatgaag
    39061 atgatcgtgt tttgcgccat gcgtatctaa atagctacta tcaaacaggg ctgaaaaatt
    39121 tgttagatgt ggctatcatt gatgaagcaa cagaaacttt agatacagat aagatcaatt
    39181 atcaaaaagt ggatgaaatt ccttttgatt ttgaacgtcg tcgtatgagt gttgtggttg
    39241 ctgatccagc tggtaaaaca caaatgatca caaaaggcgc gattgaagaa atgctgaaca
    39301 tttctaaatt tattgatctt ggcggtactg ttacaccatt aacaaaagaa cgaaaagaag
    39361 ccgtacttgc aaaagtacag tcacttaatg aagatggctt acgagtgatt ggtgtcgcac
    39421 aaaagaccaa tccaagcgtc gttggagaat tttcagtcaa agatgagtca gacatggttt
    39481 tgattggtta tcttgctttc cttgatccac caaaagaaac aacgaaacaa gcgttgaaag
    39541 ctctgaaaga acatggtgta gctgtaaaag ttctgactgg ggacaacgaa ttagttacac
    39601 gttcagtgtg ccgtcaagta ggtctagaga tcaatgaatt ggttaccggt gagaaaatct
    39661 cggaaatgag tgatcatgaa ttagcacaag tagctgaaga ccatgaagta ttcgtaaaat
    39721 tgaatcctca gcaaaaagca cgtttgacga cagctttacg taaaaatggt catactgtcg
    39781 gatttttagg agatggtatc aatgacgctc ctgcaatgaa agtggctgat atcggtattt
    39841 ctgtagatac agctgtggat attgcgaaag aatctgcaga cgtgatcctt ttagaaaaag
    39901 atttgacgat tttggaaaga ggccttcttt ctggacgaga aacttttggg aatatcatga
    39961 aatatatcaa ggctacagca agttctaatt ttggcaatat gttttccgtg ctgattgcaa
    40021 gtacgttcct gccattttta ccaatgctgc ctttacaact tttgtttttg aatctgatct
    40081 atgatgtttc ctgcatttcc ttgccatggg atcggatgga taaagaatat ttggacgagc
    40141 caaaaaaatg gaatgcatct agtattgggg aattcatggt ttactttggt ccaacaagtt
    40201 cgatatttga tattactact tacttgttga tgtattttgt tatttgtcct gctgtagtgg
    40261 gcggaagttt ccatacattg gatagttcac agcgagtgat tttcatcgca ttattccatg
    40321 caggatggtt tgtcgaatca ctatggacac aaaccttagt gctccatgca ctgcgtacac
    40381 cgaaagtacc atttatccaa agcaacgcaa catttgccat gtttgttatc actacattag
    40441 gaattattgt cggttctgtc cttcctttta caagttttgg tgctgaatta ggcttgatgc
    40501 cacttcctgg caattactgg ttatggttat ttgctacagt catcgcttat ctggcgcttg
    40561 tgacagctgt gaagaaaatc tatatccgtc gattccaaga actcttataa acgagaaggc
    40621 tggttctttg cttctgttct ttttggacca gtcgtgctaa actatgtgta ttgttttgga
    40681 aaggattaat gtatagatga aaggtgcaat atttgattta gatggtttgt tagttgacac
    40741 agaaaaaacg tatcgtgatg gctggctttg gggatttcag caatttgata tccatatccc
    40801 aaaagaaatc gttgacgctt ggggcggaaa aaactggaaa caaagttttg atattttagt
    40861 gaaagcagca ggtagccctg aaaaagtaca ggaagtccga gcgaagcgtg aagaatattt
    40921 ttatcaacag ctgacaagcg gagccatcca cttaaaacca tatgcaaaag aaatattggc
    40981 agacttaaaa gaacgtcata tgaaactagg cattgctacg accactgtta cgaaaagagc
    41041 aacagatatc ttggatcact ttaaattagc tgattacttg gacacgttta cattcggaga
    41101 cgaagtgtcg gagaacaagc cttcaccgat tccttatctg acagctttgg aacgaacagg
    41161 attagaagct agttcagcct ttgcagtgga agattcttta gtaggagcaa cagctgcttc
    41221 acgagcagga ttaggcgttg ttttgatacc tgattccagt tttgtccgaa attataccgt
    41281 cgaagaaaag gaaaaattga aacttcttgc ggaaggaaaa gatcttcgaa ctgttattga
    41341 aatgttaaat aaaaaaacag aaaaataaat atagaaaaaa taatttgaat ttcgtacttg
    41401 acttttcagt tttcctcatg tagattaatc ccaacaacaa aatatcgatt tcgttgaaaa
    41461 ggaaagtaag tttggaaact ttgcagagag gctctggctg gtgagaagag tcaagtaaga
    41521 aaagctgaaa atggccttgg agaataattt gtcgataagt aggcaaattc ggaactgttc
    41581 gaccgttaaa acgaacgcca atctctcttt ttgagatgtt gggcttgagg gaaggaaact
    41641 tccgaaaaaa ggtggtaccg cgatacgtcg tccttttact gacatagttt atgttagtaa
    41701 aaggactttt tttttgaata ttatttgcat tgaaatggta agtacgatag aagaaccttt
    41761 gacagagagc ttcggtagct gagaagaagc aaggaacgac tattggaaaa tggccatcga
    41821 gcaattcatc ccgagcatag atgtaaggcg atgacggctg cgaccgatat cttgctagag
    41881 tatgatcgta cttgaagagg agttgtgtgt gaacacagct tgaacatgag gtggtaacac
    41941 gcaaaaaagc gtcctcaaga aaatgacatt ttcattttct tgaggacgct ttttttagat
    42001 aaggaggaat tttaatgatt gaattgcgag atgtttctgt atgtttcaaa caaaaagaaa
    42061 aagagataca ggcagtagat tctgtagatt taacgattga aaaaggcgat gtttatggaa
    42121 tcgtcggtta ctcaggtgca gggaaaagta cgttagttcg tgtgatgaat ttgctgcaga
    42181 aaccaacagc aggagaagtc attgtcaatc agacgaatct gagtcagctc tctcccaaag
    42241 aattgcgaaa agaaagaaag gccatcggta tgatctttca gcatttcaat ctaatggaaa
    42301 gccgaactat ttttgacaac gttgattttt ctttaaagta ttctggaaaa tcgaaacagg
    42361 aacgtcgtca aaaagtgaat gaactactgg aacttgtcgg tttggaagaa aaagcatctg
    42421 cttatcctaa acaactgtcg gggggacaaa aacaacgagt agcaatcgca cgagctttgg
    42481 cgaatgaacc taaggtatta ttatgtgatg aagcaaccag tgcacttgat ccgaaaacga
    42541 cccttcagat ccttgcattg ttaaaaaaat tgaatcgcca gttaggttta acaatcgtcc
    42601 tgatcactca tgagatgcaa gttgtcaaag aaatctgcaa caaggtagcg gttatggaag
    42661 atggaagaat cgttgaaaaa ggtagcagca tccagatctt cagcaatcct gaagaagaat
    42721 tgaccaaaga ttttattcga acagctactc atcttgacca agcattagaa acgatcattg
    42781 cccattctgc ttttgccgat cagatcgcca ataaatggct ggtggaatta agttatattg
    42841 gaaatcagac gaacgaacct ctaatcgctc atctgtacag caaatatcaa gtaacagcga
    42901 atattttata tggcaatgta gagttattac aagaaacgcc gttagggagt ttgatcgtta
    42961 ctttagctgg agaaaccaaa cagcggaaaa aagcattgga ttatttgatc aaatctggtg
    43021 taaaaataaa tattttacaa aaaaacgatt tagatagtcg tatcaaagtg atcgaaggag
    43081 gaagataaaa tgactgattt ctttaaaaca tatttgccca atgtttattt gattccggat
    43141 gaatttgtcg aagcgacgaa gcaaacacta tatatgtctt tttggacagc attcattggc
    43201 gggatgattg gaatcatctt aggtgtgacg ttagtagtga cacgccctaa tggattacta
    43261 gctaaccgtc tgctatttga aatacttgat aagctgatca atatcattcg ttccatccct
    43321 ttcattatct tgctttctct tttagcattg actactcgtt tcttagttgg aacgacgatt
    43381 ggtgcgaaag cagctttagt gccattgatt tttggagttg ttccgttttt tgctcgtcaa
    43441 atcgagaacg cactgctaga agttgatcct ggggtgatcg aagcggcaga agcgatggga
    43501 accagtccaa tcggtatcat tttacgtgtg tatttgttag aaggtttgcc tggtatcatt
    43561 cgggtctcgg cgttgactat catcaacgtg atcggactga ctaccatggc tggagcagta
    43621 ggtgcaggcg gattagggaa tcttgctata tcgagagggt ataatcgatt ccaaacagat
    43681 gtaacgattg tggctacatt attgattcta atcttagttt ttttaagtca atttatcagt
    43741 aatcaactga ttaaaaaaat tacacattga aagtggagga gaaaaaatga aaaaaattat
    43801 tcgttttgta gtcattacat ttgtagcaat tatttggttg gcagggtgta gccaaggaaa
    43861 agcagataca actgacaaaa aagaaactat cgtaaaacta gggattattg gagaagatac
    43921 agatgtatgg gacaacgtga aagaccgttt gaaaaaggag aacattaatt tggaatacgt
    43981 gaaatttaca gattacaatc aaccaaatgt ggctttagca gatggatcga ttgatttgaa
    44041 ttctttccaa catcaattct ttttagacaa ttacaataaa gaacacggaa ctgatttagt
    44101 atcgatcgga aatactgtca atgcaccgct gggaatttat tctaacactt tgaaggatgt
    44161 agctgatatc aaagacggag caaaagtagc aatccctaat gatgttacaa atggcggcag
    44221 agctcttctg ttattgcaaa ctgctggatt gatcaaagtg gataaggcaa aaggacaagc
    44281 gcccactgtt tctgatatta ccgagaatag aaaaaatcta gacatttctg aattagatgc
    44341 ttcccaaaca gcacgtgctt tgtcagatgt agacatttca gtgatcaata gcggtgtagc
    44401 tgtagatgct ggatttaatc ctgcttctga tgccatcttc ttagaaccag tggatgacca
    44461 tgcacgtcct tatgtgaata tcatcgttgc aagaaaagaa gatgaaaaca ataaaacgta
    44521 tcaaaaaata gtagacgctt atcaaacaga agacacagtc aaagtaatcg agaaaacatc
    44581 aaaaggatca agcgtgcctg catgggaaac attcgggaga aaataagaaa aatgactagc
    44641 ataggaggaa ttttttatga caacaacaat ctcaacaaac caattgaagg atctaatcca
    44701 atatcgttta ccagaatatc aagcacttgc tctagatatc cacgaacatc ctgaagtaag
    44761 taactatgag ttttattcct cagcagcgtt gattaaacaa ttagaaaaag aaaggttttg
    44821 ggtagaaaaa gaagtagctg gacaccgaac agcctttgat gcacgttata tttccaataa
    44881 acccggtccc acaattgcct ttttggcaga attcgatgct ttaccaggta ttggtcatgc
    44941 atgcgggcat aatttgtttg gtacttactc cgtattagcc gccagcatag tgaagcaatt
    45001 cattgatgaa actggtggag aaatccgtgt atatggcacc ccaggggaag aagggggcga
    45061 gaacggatca gctaaaggca gctttgttcg agaaggtttt tttgaggatg tcgatgtagc
    45121 gttatgcgtc catccagctt ttcgttatgg gaaaacgaca gaatctttag cgaatgatcc
    45181 agtcgacatc aaattttacg gtgtcgcttc ccatgcagca gcagcacctg aaaaaggagt
    45241 aaatgctttg gaagcattga tccaagtgtt taatggaatc aacgctctac gcttgcaatt
    45301 gccaaaagat gtcaatatcc atgggatcat tacagacgga ggagtagctg ccaacgtcat
    45361 tccagattac gctgctggac gtttctattt acgtgcagga aatcggcaaa cattagataa
    45421 agtatatgaa aaagtggaaa atatcgtacg aggtgctgcg ataagtacgg gcacgacttt
    45481 tgagtttgga ttgttccaaa atgcagttga tgatgtcatc gttactcctt cttttgatga
    45541 cttattcttc cagcatgcaa aagaagcagg tgttccagat gaagaaatag aaacggaaca
    45601 aagaacaagt ctcggttctt ctgatgtagg aaatgtcagc caagtcatac caacaatcca
    45661 gcctacggta gcaatctctg agacatatat tgccggacac acagaagaat tcaaagctgc
    45721 tgctaaaagt gacaaaggcc ttgcttctat tgcgattgca gcagaactac tcgccaatac
    45781 agctcttgat ctgttgaaag agccaaagct attagaaaga atcaagcaag aacatcaaga
    45841 aatagtagca agaaaaaaac aagagggcgc tactttctag taaaaaataa acttgtgtgt
    45901 gaaagttata aaaataattt tcagaaaaat gaaaaaatat tttctttttc attgacaaat
    45961 gatcttatgg aaggtaaact tcgtacaagt caacttttca cgcagtagcg gttaggtgtt
    46021 ttgttcagaa agtcggtggt cgctgtgaac cgatcaagcc taattgacga attacatgga
    46081 aagtccatac atttgaataa aagtttgaaa gtgaaaaaca gatctgtttt taattaaggt
    46141 ggtaccgcgg agtcattcgt ccttagttta aaacaggtct ttttattttt ataaaggagg
    46201 aatcgcggat gaattgcggc ggagtaatcc gatatcttaa cttttttaga acataagaac
    46261 aactaaaaaa ggagagatta tcatgggaaa aaaaggaaca ccaagttttt tagaagcaac
    46321 acttgtatta atggttattg ttttaggcat tgcgattggc gttgtcggat taaaggtctc
    46381 acctaatatc acgatcttag cagcgatcgg aatggtcatg ttgtatgcag tgataaaaag
    46441 atacccgaca gattggctgc acgaaggaat catcaatggc atcaaaccag gaatcatccc
    46501 aatctttatt tttattttag tcggttcatt gattgctgta tggatacaag cagggatcat
    46561 tccaactcta atggtcattg ggttcaaatt gatcagtgtc cagtggtttg tcccttctgt
    46621 atttgttgtg tgtgcaatcg taggtagcgc tgtagggagt gcctttactg ttatgtcaac
    46681 gattgggatt gcatttttcg gtattgggac aacactaggc ttaaatccag cgctggttgt
    46741 tggagctatt gtttcaggtg ccgtttttgg agataagatg tctccattat ccgaatcaac
    46801 aaatctagca gcagctattg ttgatgccga tttgttcaaa cacatcaagc atatgatgtg
    46861 gtcaactgtc ccggcatttg ttgtttcttt ctttttattc atgattttag gacacacaag
    46921 taaagccgca agtctttcat ctattcgtga agtgactgat gttctagaag caaactttac
    46981 tatttcaatc tggtcactga ttcctttatt tttaatgtta ctgtgtgcat ggaaaaaggt
    47041 gccggcaatt ttaacgattt tgttgaatat cgctgtagca gtaggaatga tttttattca
    47101 aaacccacaa acttccttaa cgaagttggc tggggtcatc gaatcagggt ttgtttcaaa
    47161 tactggaaac caacaaatcg attcactgct tagccgcgga ggaatcgaaa gtatgatgcc
    47221 tactgtgtca ttgattattc taactttgtc attaggtgga ttgttgatcg aatttggctt
    47281 gatctcaacc gtgatggaac gagtatcaag aaagatgacg aacacaccaa atttgatttt
    47341 tacaacattg atgacaagta tcggagtaaa tatttttatt ggtgaacaat tcctatctgt
    47401 tattctgcct ggtaatgcat ttaaagaaac ttatgaaaaa gcaggatttg atccaactgt
    47461 attaggtaga acgctagaag atggtggaac agtaatcaac tacttagtgc cttggggaat
    47521 tgccggaagt ttcgtagcaa gtacatttgg tattcctaca ctcacttact tgccattcgt
    47581 atttttcagt ttgctttctc ctgtgttttc gatggtcagt gccttcacag gttggggaat
    47641 ctcaaaaaaa tttgtgaaac cgcaagaagc agtaagcaag acagcgcaat tagaacaata
    47701 aaaacataaa aagggattgt gacaagactt ttgtcacaat cccttttacg aataaacggt
    47761 attcaaaagc cagtttctcg gtattaagcc aaatttatac aaaaatgtgg aaagccattt
    47821 cacaaaattt ttcttaatac gcgaaactaa gcgacttttt cacaacctct cgataaacgg
    47881 tgttcaatca gctgaccctc ggtattagct caaaaaatgg caaaatatga ggaactattt
    47941 ttccatttgt ctctctaata ctcaggtcag aacgctgatt ttacaacctc ttaataaacg
    48001 gtgttcaaaa gcttgcatct gcggtaataa gcctaaccaa acgaaaaata ggagaagcta
    48061 tttttgtttg gctgttctta ttacttgatg cagggcagct ttttcacaac cccttattca
    48121 cggcgttcca gaagtagctt cttcttattt cttgaagctg ataacttctg gaacatcctc
    48181 tttttttatt tggacggata atggtcattc catttctttg atcatttttt tcattttgtc
    48241 gatctcttct tcttgtgcct gaatgatttc ttccgctaat ttttttactt caggatcttt
    48301 taaatcagct cgtttacttg ccaggatagc gatagaatga tgaggaatca tggccttcat
    48361 ccaagctgtg tcgttgacag tagtttggct tcgtaccata aacaaggaac cggcaaaaac
    48421 aagaagacta gcggctaaga tcataacatt tatctttttg ttttcataca ttttccacat
    48481 gaacaacagc atgatgactg ccatgactgc acccatcatc aacgccatga atactcgtgt
    48541 acgactgaag aagatatgat cgagcgcata gacattcaag tacatcatac caaacataac
    48601 taccgttgaa acaccgatca tcaacaaaaa cttccaatac tttttcattt aaaacaaact
    48661 cctttttttc attcctttct tctatcatgt catgagcatg gagaacaatc aattattttg
    48721 cacagagggt ttttgaacac cgtttaccaa gaggtcatga gactgtttat tcggaaaaag
    48781 gagttgcaac aagacttttg tcacaactcc tttcttcaat agctccatct attccacata
    48841 aataaaataa aattttctat cgatttcatt cattcgtcaa tacgaacgca tcattctttt
    48901 tttgataact gaaataaatg aacaggtaaa gcaggccagt caatacagtt accccaatgg
    48961 agttcgtgaa caggatacca aggaaaatca atcctaagac agctaatgca aaaaagctga
    49021 ttcctttatc ccataatgtt ccttttctta gtgccagtac aaaggatgcc gcactgataa
    49081 tgatccagat caataatact gtatacgaaa gggaacctgc cagatagcca aataatttgt
    49141 caccgacaaa ataagaaaga gctacaccaa gataaagaac actggtacaa aataaaacag
    49201 cacgttgcgg tacttgatgc ttgtttaaaa cagctagttt tctagaggaa ccttttcgat
    49261 ccttcaaacg gaagaacaaa attcttgaag atgcatacac acccgaattg atagatgaga
    49321 acaaagcaag gacaatcaca aaattgacga tatccgctgc aaatgggata ttcatttttt
    49381 caaataccat cacaaaagga ctgacattcg tgcctgctaa agtattccaa ggaaaaagaa
    49441 tcaaaagcaa aaacaaagga atgatgtaaa aggaaatgat tcttcccatg acacctttga
    49501 ttgctttagg aatcgcacgt ttaggatcat ctgcttcgct taccgtgata gcaatcagtt
    49561 cagaaccgcc gtaagaatag ataactacaa gcaaagaact gatgattccc ttcataccat
    49621 gaggagtaaa cccaccatgg tcagtcaatt gctgaagggt agggaaaacg cctgtgccca
    49681 acatttgctg accgacaaga taagtagcaa aaatgatcag taagataatg acactgattt
    49741 tagcaaaagc cagccaatat tctgtttctg cgaataaccg taccgaataa agattgatga
    49801 tcgtagttaa aacagccaac ataaaaacaa atacccaggc tggaatgtgc gggaaccaca
    49861 actgcaaaaa ggaagcagct gcaacagctt cagcgatgat attgataatc cacatagacc
    49921 agtagaccca atcagtaaaa tcagcagaat gagcacctag ataagggcgt atcaaaccag
    49981 ataatccgtg atgcgtgtcg ccaccgctta aaacgatttt tcccagacct ttcatcacga
    50041 tatataaaat cagcccacca atcaaaaaag caatcaatac agaagggccg gcgatcccaa
    50101 tcgcttcgcc acttccttta aatagacctg caccgattgc tccacctaaa gctaacatgg
    50161 tgatatgtct ggagctaagt tgttttttca attttttatc ttccataaat gacctctttc
    50221 ttttaaattc acaatcaaaa tttatattaa gaataaatag caatatttaa caatgactaa
    50281 tgataacaaa ttttcagaca attacaacta ttcagaaaac ttttaggcga attttaatag
    50341 aagaatgatt tatttcatca tattttcata caaatgttaa attttcatac aaatatagga
    50401 aacgttttac tggcttttta ctatttaaac aggctatcat aggaaaaagt gacagagaaa
    50461 gtgaggcgga aaagatgaac aaaaatgacg tactgctagt tgcaaaaaat aaatcacttt
    50521 attttttgcc ttccatccgc tctttagatg tttggtccgg aaaagattta ctacagtcgg
    50581 aattcattaa atcagctaga caacagttgg caaattatca gctgatcgtt tttttagatt
    50641 atggatttga tatggagatg gctcgacgcg tgcgcccatt taccaatgca aaaattgtgt
    50701 tatttttttg gaatcacttc aaagcagaac acgaggcgat gctgaagcag gcacaagatg
    50761 aaccggcaat cgatgagatt tatcattttg attttttgga agcaaaagaa cttgggctga
    50821 aacataatag ttcattttat tcgaaacata tgaatttttc ttctagcgaa ccaaagacgg
    50881 atctattttt tggtgcaacg aataatggga gaaaagaacg agcagaaagc tataagcagg
    50941 aatttataaa aagagatgtt acgaccaact atttcattct tccacatcga gggaatgaac
    51001 aacctggata tcttacttat gaagagtacc tgaaaaaaac aggggaatcg cgcgggattc
    51061 tggaactgtt gcgggagggt cagcaaggag tgactttgcg gacatttgaa tccatgtatt
    51121 ttcaaaagaa actagtgacg gataatcaag cgattgctca ttatcatttt tatcgtcctg
    51181 aaaatatttt tttgcttcga gaacgtgatt tagatgaatt accggaattt ctgcatacac
    51241 catttaagcc aatcctatca gagctattgg aattttatga tgcagaaaat tgggcaaaac
    51301 gatttatcac taacgattcc actatttatg agcaatacga atatgacctc tcctctgttg
    51361 aaaacaaata aaaaacctac attttgaaga gattatgtac tgaaacaaac aagcacaatt
    51421 cttatggcat catgagagtt gtgttttctt tttgatcttt tattgggctt aaaaaaagag
    51481 aaccagctgc tgattcagct gattctcatc agagagcctt ttattctgcg ccaaggattc
    51541 cttcttttgc atggactaat tgggtcaaaa aggcacaata tggtgtagca atatcatatt
    51601 tttgtccttt tcgccaaatg gcaccattga tgtagtcgat ttcagtcaaa cgatggtttt
    51661 tgatcaggtc ttggtacata gatgggtaat gcaacccgat gttgtcagga tcataagtag
    51721 aagcaatatg ttggtacact tcttcttgat ctagtatgat tccttctttt gctgcgatag
    51781 aagcaaattc agatacgatt gttcgaacca tgctctctgc cgctttttgc gcgcctagtt
    51841 cagcaatatt acaatccaaa atcgtgcata aaccattcaa tgttccattt acacaagctt
    51901 ttcgccaaat ggaataacga acgttatggc tatagatcgg atttaaattt gcttcttgaa
    51961 agacttctac aacttttttt gtaaatgctt cgccttctgg ttccaagttc tctagttcga
    52021 tttctccatc acctaacaat gtgacttttc cgggaccagc tagaccagct gtccacatcg
    52081 tgatcccaaa taagatattt ttctttggca catatttttc cagtacatct tcatgaccta
    52141 atccatttag taaacacaaa acataagtat tgtcagtaat aatggattgg atcgaacaaa
    52201 acatatcatc taattgattg gcttttgtta atgctacgat caagtccacg tgttcgttgc
    52261 tttcgatgat ttcttctggt gaatagattg gcagttttgc gaccacttct ttaccgttaa
    52321 agtcagcaat caacccattt gtacgaatgg cttcaatgtg agcaggccaa cgatcgatca
    52381 agaggacctc atttccgctt tgatgtagca tcaatccgat tcggctgccc attgctccgg
    52441 ccccagctat tgcgattttc atgttttttc tctcctttat tcaatgagtt cccttttagt
    52501 atatagaaaa atggaagaaa atagcattgt tttgtttgaa aaaaatgaaa aaaagtgtct
    52561 tacttaagaa gaaagtgagg gatacagatg gatattttgg cggcaattga ttcatttaaa
    52621 ggaagtgctt cttcatttga actaaatcag gcagttctaa atggctggga agatcgtaaa
    52681 ggaagaaaaa tcaatgtgcc gattgcagat ggcggagaaa atactgcaga agcaatctat
    52741 tacgcgctca ggggaacatg gcgtaccatt gatgggatgg atctcttatt ccgtcagcga
    52801 aaaatcagct atttattgac aacttggcaa ggaaaaaaca ccgcagtcat cgaatctgca
    52861 caagcattgg ggttagatct acttgataaa ccaacggatc aaactatccg acttgcttcc
    52921 agttatgcct taggagaact tattaaagat gcactgcttc aagaagtaga acaaattatt
    52981 gtcactttag gaggaagcgg gtgtagcgat ggtggtctag gattactcca atccttgggt
    53041 gcttctttag aagggtacac agaaggaaat cctttattat ctactaaaaa acttcatttt
    53101 gatcgaagaa acgttcttgt ttctaaagta aaattgatta tcgctgcaga tgttaccaat
    53161 ccatatacag gtcaacaagg agctgcttgg caatttggaa gacaaaaagg aggaatagat
    53221 acgacgctag ctttcttaga taaacaagca ggaaagattg ctcagcaaat aaaagatttc
    53281 tgggaggtgg atttagatga tattccagga agtggagcag caggcggtat tggtggagcc
    53341 ctttttttgt tgggagcgaa gatggtttct ggtttcgatt tagtcagcca aatcactgga
    53401 ttggaaaaaa agatcgaaca agcagatgtc atcattacag gagaagggcg gatcgatcag
    53461 caaacgatcc acggaaaagt tccatacggt gtagcagtgc ttgcaaaaaa atatcgaaaa
    53521 ccaataatcg ctatttgcgg aagtagagac caagagctag aaggactttc tgattttctc
    53581 ccaattgtct taagtattca tttgggtcca ataggtttag aagaagcaat ggaacacagg
    53641 caaacattag aaaaaacaaa aatcttagga cagacactta gcagattttt caccatcctc
    53701 ccgaagtagt tgacaaaatc attttcttta tgctatcgta atttcaaata taggacaaag
    53761 tgagggatcc gtgcattgaa aaattaagtc gattgatgaa agctgcattc agactgtacc
    53821 ttgtggagac aaggaagcag tgtgcagatt ttcgggattg gcttggtttt ttcgatgtat
    53881 gggtcttttt gtgtgtatcc aacccttgca tcctctttag ccagttctga aaataggact
    53941 ggtctttttt tacgaggtga tggaaaatgt ctaaaatcga aataaaaaat ctgacattcg
    54001 gctacgacag ccaaggcaca ttattatttg aacaagcaaa tctaaatttt gacacacaat
    54061 ggaaactagg acttatcgga cgaaacggtc gaggaaagac aactttactg aatattctac
    54121 aaaacaaact accttatcaa gggcaagtaa tccatcagca agaatttgcc tatttcccgc
    54181 aacagacaaa agataaagaa cgtttaacct attacgtgtt aaatgatatt acggattttg
    54241 agatatggga aatcgaaaga gagctccaat tgatgcaaac agatcctgaa atcttatgga
    54301 gagaattcag cacactatcg gggggagaga agacaaaagt cctactggca cttttatttg
    54361 tggatgacac tcatttcccg ttaatcgatg aaccaacgaa tcatttggat atctctggta
    54421 gaaaacaagt agcggcttat ttgaaaaaga aaaaacaagg cttcatcgtg gtcagccatg
    54481 accggggatt tatcgatgaa gtagtggacc atgttttagc aatcgaaaaa agtcaactgg
    54541 aactttatca agggaatttc tctatctatg aagaacagaa aaaacttcgt gatgaatttg
    54601 aaatggctca aaatgaaaaa ttgaaaaaag aagtcagtag gctaaagaaa acagcagctg
    54661 aaaaagccga atggtctcgt tcccgagaag gagataaaac aaagaaacaa gtcggattca
    54721 tcgatactga atctagacga gtgaataaag gagcagtggg tgctgatgct gcacggacga
    54781 tgaaacgatc caaagcaatc gtgaatcgga tggagaccca gatcagcgag aaagaaaaac
    54841 tattaaaaga tatcgaatat atcgattctt tgacgatgaa tagccaagcg tctcaccata
    54901 agcgactttt aagcgtagaa gatcttcaat tagggtatga aaatctgtta ttcgagccaa
    54961 ttcattttac aatcgagcct catcagcggg tggcgatttc aggtcctaac ggtgcaggaa
    55021 agtcatccat tatccattat cttctggggg cattcaacgg caaggttata ggagaaaaat
    55081 accagccaaa acatctgagc attagttatg caagccaaaa ttatgaagac aatcgaggaa
    55141 cgttggcgga atttgcagag aaaaaccaag tagactacca agcatttttg aacaacctcc
    55201 gaaagcttgg gatggaaaga gatgtttttc ataacaagat cgagcagatg agtatgggcc
    55261 aacggaaaaa agtggaattg gctaaatctt tatcacagcc agctgaacta tatacatggg
    55321 atgaaccatt gaattatttg gatgtcttca atcaagaaca attagaacaa ctgatcttga
    55381 acgtgaaacc tgccatgtta ctagtggaac atgatcaaac cttcctggat aaagtatcta
    55441 ctgagattat ttctcttgag agaatctaac ccttgtaatc tcaagaacaa caaggtatgg
    55501 ttaaggggaa ctttgccaac gaaggagaga atcacatgct ttatgtaacg agacacggag
    55561 aaacaacttg gaacgcacaa ggattagttt gcggacgggc agatgtgccg ctgactgaaa
    55621 aaggacagat gcaggcacag aaactagcag aaaaggtagt agatttgcct gttccaatca
    55681 cgaaaatcat ccattcccct cttcaacgag caagagatac agcacaagca gttgctgatc
    55741 gtctcagctt gccgctgacc gttgatgaac gattggtaga aatggatttt ggggattatg
    55801 atggactgcc aagcaaagat gagaactttc aaaaagcacg tttagcgttc gctgtacgat
    55861 tcccaaatgg ggagtcagtc ttagatgtgt atgcacggat cgttccttta ttgaaagaat
    55921 gcatcgaaga cgaagaaaat gtctatctgt tagtttgcca taatgccttg ataagagtga
    55981 tcaatgctta ttttcatccg atgcctaatg aggggttctt tacattcatg gtagataata
    56041 cagaattggt ttcttatgag taattatgtt tttgtgttat ttaaataaaa tatttcaagg
    56101 cactgctttt gcttagcagt gcctttttta ggttatgaca attcttttga taatataaac
    56161 tcaaccttta gaaatgaaat ttaattattt taataaaagt attgaaattt tgtttcttta
    56221 gatatatact ctagtcatag aaatgaagac ggatacagaa aaagagggat aatatgtttg
    56281 gcttttcaat atttatgaac gattctttgt cagaagagaa gaaacagtat atccgccaaa
    56341 tggcatccaa tggctttgtc ggtattttca cttccatgca tataccagaa gatgatgttt
    56401 cggcatatca aaagcgattg atcgaactag gaagcgaagc gaaaaagaat aaactagaat
    56461 tgatggtcga tatttcagga gaggcattgg aacgagcggg tttttcatta gaagatctaa
    56521 cgcctttgaa agcaattggt gtaacaggtt taagaatgga ttatcatatt tccaatcagc
    56581 agattgccga ctggtcacat caattgaaaa tcagtttgaa tgctagcacg atcacgccta
    56641 aagatatcga tgaattgaaa gaagcagaag ctgatttctc acagattgaa gcttggcata
    56701 attattatcc acgtcccgaa acagggctgg acaaagaatg gtatcagaaa aaaaatcaat
    56761 ggctcaaaat tcaaggtctc cttgtccaag gttttgtacc aggagataca gagttgagag
    56821 gcccactata tcaaggattg ccaactttgg aagaacatcg agggatccat cctcttgcag
    56881 gcgctctaga tctattagca agtaacacgg atattgttta catcggagat gcaggtctgt
    56941 cagaaaacgt tcaggagcaa tttgcttctt tccaaaaaga acagacagtc ttgctccata
    57001 cggaacctgt agatgaagaa ttttacgaat atatattagg gaagcatacg aatcggcaag
    57061 acgatgcaag agatgtcata cgaagcgtag atgcacgttt tcgagagatt ccgcctatcc
    57121 cagcaagaaa tacagctacg cgtatgaaag ggtctattac tttagataac gaaaagtatt
    57181 tgcgctatat gggtgagatc caattaacca aatatgattt gccagcggat gaaaaagtga
    57241 atgtagtagc taaagttatc aaagaagagc taccattgat caaccaaatc aaagctggta
    57301 tgaactatca atttataaga aaagaaggaa gataaaaatg agcaaaatca atttagagaa
    57361 cctaacaacg gaacgcagaa accaagaaac ttttggatta gatgagatga gtatttctca
    57421 agccgttcaa ctaatgaaca aagaagatca gaaagtggcc gctgcggttg aaaaagaact
    57481 gacaagtatc gaaccagtga ttgctaacac gattgagtcc tttaaaaaag gtggacgtct
    57541 gatttatatg ggagccggaa caagtggaag actaggtgtg ttagatgctg ctgaatgcgt
    57601 accgacattc ggtgtagaac cggaaatggt cataggtcta attgctggtg gagaagaagc
    57661 aatgactgta gctgtcgaag gggccgaaga ttcttatgag ctagggcaag aagatctaat
    57721 caagctgaat ttgacaaaaa atgatatagt tattggtatc gcagctagcg gtcgtacacc
    57781 atatgtgatc ggtggattag attacgcaaa atcgatcggt gctgtcacag gaacgatttc
    57841 ttgcaataaa caagccgaaa tcagtaaaca cgcggatttc ccaatcgaag tcgatgcagg
    57901 tccagaattt ttgacaggct ctactcgatt gaagtcagga acagcacaaa aacttatctt
    57961 gaatatgatt tctactatca gtatgattgg tataggtaaa gtattcaata acttgatggt
    58021 cgatgtcaaa ccaacaaatg aaaaactaat cgaacgctca aaacgaatca tcatgcaagc
    58081 gacagaagct gattacgaaa cagctgaaaa gtactttgaa gaagcagatc aaaatgttaa
    58141 gctggctatc gtcatgattt tgacaaatag tgataaagag gaagctcaag caaaattagc
    58201 tgaagcaaac ggatttgtaa aagggacctt atgaggggga aaagaccatg gcaaaaaacg
    58261 aactgacttt ggaagaactg gcaaaacaaa tttataaagg ggcaggcggc atgggcaatg
    58321 tagaaagcat tgtccactgc atgactcgtg tgcgtatgaa gatcatcaat gatgatcttg
    58381 taaatgtctc tgaattaaaa gcgattccag gagtgcttgg tgtagtagaa gatgaacagc
    58441 ttcaagtcat catcggacct gggaaagtga acaaagtagc acagcaaatg gttgatttgg
    58501 caggcgtgaa attaggagaa aaactttctt ctgtaagtac agctacagca aattcatcag
    58561 catctggaaa agaccgtgta aacgaacggg cacaagaaat gaaagcggca caaaaatcga
    58621 aacaaaaacc atctaaaatc aaatcggtat taaaagacat ttctaatatc tttgtgccaa
    58681 tgattcctgc atttgtcggt actgggatcg tggcagggat tgccgcagtt ttagcaaact
    58741 tagtcacagc cggaacactt gatgcagcaa catggcaaca atatatcgat gtgatgaaca
    58801 tcttgaaaaa cgggatgttt agctacctag ttatctatac aggtatcaat gctgcacaag
    58861 tttttggtgc aacaccaact cttggtggag ttatcggggc cgttgtgatg ctgacaggga
    58921 tgaatccgga agcaccactg aaaaacttat ttactggtga agctcttgca gctggacaag
    58981 gtgggatcct aggtgtcatc ttcgctgttt ggctattgtc aatcgttgag aaaaaactgc
    59041 ataaagtagt acctgatgcg gtagatatca tcgttacacc aactctttca ttgattgcca
    59101 tcggtctgat tgaaattttc ttcatcatgc ctttagcagg tttcatttct gatggcatgg
    59161 tcggtgggat caactgggta cttggcgttg gtggagcatt tgccggcttc gttctaggca
    59221 cattattctt gccgatggtt atgttcggac tgcatcaaat tttgactcca atccatgtgc
    59281 aaatgattga tgaaactggt cgtacgctat tgcttccaat tcttgcgatg gctggtgcag
    59341 gacaagtggg tgcagcattg gcattatgga ttcgctgcaa aaaagataaa gaattaacag
    59401 aaatgatcaa aggagcttta ccagtcggga tcctaggaat cggcgaacca ttgatttacg
    59461 gagtaacatt acctctagga cgcccattta ttactgcttg tatcggtgga ggtatcggcg
    59521 gggcagtgat cggaatgatc ggaaatgtcg gggctatcgc tattggacct tcaggagccg
    59581 ctttgatccc attgatctct gacggtaaat ggttaggcta catccttgga ttattggcag
    59641 cttatgctgg tggcttcgtt gctacattct tcttcggtat tcctaaagac cgtttagaaa
    59701 aagaagaatt agcgactgaa acagccacag taaatgaagt gcctgaaaaa gtagtacctc
    59761 aaacttcagc tgaaagtgtg actatcctct catcagttgc tgacggaaca gttgtttctt
    59821 tagataaagc aagtgatcca gtttttgcac aaaagatgat gggtgaaggc tactttgtag
    59881 aaccagaaaa cggacatatc tattctccag ttactggggt agtcagctct gtcttcccaa
    59941 cgaaacacgc tattgggatc acaacgccaa atggtttaga aatcttgctt catatgggaa
    60001 tcaacacagt tgacttaggc ggtacgccat ttgatgtgaa agtaacagaa gggcagcaag
    60061 tgacacctga tacgcttgta gcagaagctg acttagaagc gatcaaagca gcaggcaaag
    60121 aaacatccat gatcgtcttg gtaaccaata tggatcgtgt aaaaaacttt gtcttggaaa
    60181 aaacaggtaa agcaaaagca aaagcacctg tgatggatgt caaagcttaa tatgatagaa
    60241 acaatagaaa cgaaagaaga aactgaaatc tatttttcag cttcttcttt tttattattt
    60301 attgttttct gctaaaatct gataaattcg tgtcacatca gatggtgttc cccgtaattc
    60361 ataatctgcc aagaaaggaa agccaaatgt ttgtgcatat tgtttggctg ttagacaata
    60421 ctgattattg aagtttttat ttccgctacc gactacacca agacaaagtt tatgattgtc
    60481 ttcatgttcc agatattcac gcatcgtttc ggtcaagatt tctgtatcac cgttatctaa
    60541 accatttccg ccgtctaaat aagtaggaac gaaagtaaaa aatggttctg tttctttcgt
    60601 aaaatcacta ttttcatgga tttcttttaa agtaacttca ggattgacag gatcttctgc
    60661 atgcattttt tcagcatatt ctgctagatg tttggcaaag gcacgcgtat ttcctgaaat
    60721 cgatatatat aaaatgttca tggatcatcc tccaattctt tccttttagt atagcatctt
    60781 ttttaagttt tttggtggtg taatttggaa agggactagg cgctttgctt gtataatgag
    60841 aaaaaagccg gtagaattat atttctatta aagtttttat taattaagta aaaataaaaa
    60901 aggtttgtta tactttttga taaaagcggt tttattttgg aggatgctta catggataca
    60961 gagcaacaga aaaaaggaat tggtttaatt ccacttgctg ccttagtaat tggttcagcg
    61021 attggaggtg gcgtatttgg tattatgacg gacatctccg gatcggctgg cggcccagct
    61081 attttcagtt gggtactcgt aggaacagca atgttgatgc tctccttgtc gatcaataat
    61141 atcaataaaa aacgtccaga tttaaaaggc gggatcttta gttacgcaga agaaggtttt
    61201 ggccgtttcg ctggttttat cagcggttgg ggttactggc tcacatgctg gttaggcaac
    61261 gtagcttttg caacattgtt gatgagcgca ttaggatatt tcttcccagt atttgaaggt
    61321 gggcaaaatc tggcatcgat cgtgatgagt acagtctttt tatggggata tgcatggcta
    61381 gtgaatcggg gagtcgaaaa tgctagtttc gtcaatgcta tcgtcacatt ctgtaaatta
    61441 gtgccattat ttttatttat tgtagtagct atcagcacgt tttcgatcca acaattcatt
    61501 gataactttt ctagcgacat tatattgaat gcgaatggac agccgatccc gttttccagt
    61561 caagtattga gttgcatcat ggtgatgctt tgggtatttg ttggtattga aggagcctct
    61621 gtcgtaggtt cacgagcaaa aaaacaatca gatgttggga aagcaacgat tctagggata
    61681 ttcggcttga ttattattta cgtcttggtt tccatgcttc catatgggac aatgacgatg
    61741 aatcagctgc aaacgatcaa ggcgcagcct gctatgggct atgtgtttga aatgatggtt
    61801 ggtaaatggg gagcagtcgt gatcaacggc ggtttgatca tttctttagt tggtgtttgg
    61861 ctctcatgga cgatcttacc gattgaaaca atgggaaaca tggctgaaga cggacttttg
    61921 cctaaagcat ggggaaaact caacaaaaat ggggcaccaa cttatgctat cgtattgacg
    61981 acgttatgta cgaatgtctt tttactttca ttattattca ctgacagtgc ttataacttt
    62041 gcttacacac ttggtacagc agctattttc ttcacgtggc ttttcttagg attgtaccaa
    62101 atgaaacttt cttatcagcg gaaagaatgg gtccaatttt tagtagggct tttagcaagt
    62161 gcctttcaag tatgggctat gctagtcgta gcctttaatg aagtaatgct tgttcttgtt
    62221 cttttcttgc caggcattta tttttacctc aaagcacgta aggagcaaaa cgagcctgta
    62281 caacatatcc acttagataa gattttactt ataggtgtga cgatggtcgg agtgattgca
    62341 atggttttat ttgccactgg gatgcttcac gtatagaagt ataaaagaag tataaagaga
    62401 ggttgtgagc ctgtcgttta ttcaaaaaca tactaggaaa ggagctgtga cacaaatgtt
    62461 gtcacagctc ctttttcaga ataaacggtg tttaagagac agccaatcgg caataaggct
    62521 gaacgtctct ttcacagcct cttttttgat gagtcatcat acattttttc gcttaaatac
    62581 gagatatcct aatgctaaga acaaaacgat gtaagctaaa ttaccgaata gcatttgatt
    62641 agtagaaaga tgagtcaatt gcttcatgat ctcttcgttt ccaatttggt tttgcagatt
    62701 caacatactg atcggattcc atttgatcca ctcccatttt tggatgagtg caataaggat
    62761 accagatatg attgacgaag caaaataaaa gacgattcct gctgagatgg atgcgccgga
    62821 actattgata aagcaagcaa tcatcaacac taaacttaaa atcagccata aaccgatata
    62881 actacccaaa gtataagtga acaagctttg gatcagtgtt tgaccagtag aaaccttctc
    62941 cgtgaaggaa atcgaaggga aaagaatcag tttcatcaac acagtgacaa taatagctaa
    63001 aaggtataaa taaacagaat aaataactaa tgtgatccat ttgcttacta atatttctcc
    63061 tcgagaatac tttcgataaa ggagattttt caatgtacca tattgtgctt ccatcgaaat
    63121 gatcgtgctt gcagctgcaa tcatgataaa gacaatccaa gatgtggcac taaaaagctg
    63181 agcagtcatt tcaactggat caatgatcgt cgtgtatttt ttggctagaa gtgctgttcc
    63241 tatgagcaaa acaactaata gaacagataa aacagcagtt gactttttct tgatcaattt
    63301 aaataattct tgttttacaa gcaatgtcat ttttcttctt ccccctttac tgaatccaga
    63361 atttccaata gagaagattc caaatcattc gtcagatgct ggacgtctgt cacatggata
    63421 ttttgtgcag acaatagctg gatcacggtt cctaataatt cttcgttttc ttttaaaagc
    63481 ttgattttat caggatcttc caaagtatat ccagcttctg tcaagatcgt tcgagcagct
    63541 agatcatttg ttgtagagat aacgatgaaa tcttgcgctg tttggttcaa ttcagccatt
    63601 gtacattgtt tgatgatctt tccttgattg atcaggatca gctcatcgac cattttttca
    63661 agctcgctga gtaagtgact ggagatcaag aaagaggtgc cctgagtagc caagtcggta
    63721 atgatttttc gcacatctct tgttgcttgt ggatccaaac cgttcatcgg ctcatctaaa
    63781 ataacgatag acggatggtt cagtaaggca agagcaattc ccaatttttg tttcattccc
    63841 aacgagtagg atttcgcttt tttgtgaatg tacttttcca tcttgagctg attcacgata
    63901 gtgtcgatag cagatacatc gttggtttca gagaataact tcagatgatc atagcctgtt
    63961 aagaatggat agatacccgg gtattcgatc aatgctccta cttgttccaa ccctttatga
    64021 ctagagggag aaatctcatt ttcaccaata cagattgttc catctgagta atggatcagt
    64081 cctaaaatag ctttcatgat cgttgttttt ccagcgccat taggacctac taatccaacg
    64141 attttgccag gggataagct aaaactgaca tcgctgaggg cttgtttgtg accaaagttc
    64201 ttggataatt cttggaccga aagacttttc aaaaatattc actccttttc tatagatagt
    64261 acaagcatac acctgatgag tggagaagtc gctaggtcat tggacttatt tcttattttt
    64321 tcataaaaaa attgaatatt aaataaaaag gcttattttt tctgatcagt acttattcca
    64381 agtgtctagt ggccgctagt aatcttgatg ccaatgatac tgatgagtag catccccaca
    64441 aagaaccaag tggaaagatt cagcttatca tgaaacaaga ccacaccaat tatgaccgaa
    64501 ccaactgcgc caatccctgt ccagatagga taagagagac tcatcggcaa acttttgatt
    64561 gcctttgaca aaaagtaaaa actagcaatc atgccaatca ctgtgtaaaa agaataattg
    64621 attttagaaa aaccatcact ccatttcatc atcgttgacc agaatacttc caaaattcct
    64681 gctatgatta attccatcca tgccatactt acattcctcc ttaactataa aaaaatacac
    64741 aaaaagagga aaatcatccc ttcaaagacc taatggaatg atcatcctct ttatcctacc
    64801 cggtggcaga ttgtatatct ttttctgtag tatagcacac ctatccagat atcggcaatc
    64861 tcatactttt tagtaaagtg taccaagcac tgacggatat atcgttagcg gaaaagaaga
    64921 tgaatgcttt gcaatttcat agaaagaagt tgttatttat taatcattta aaaaaaacag
    64981 taactagacc ttatttctat atattatcgg gcaataaatt aaaaaaagct ttaccttaca
    65041 ataagtaaga taatttttgt gataaaaaaa taacgaaaaa tatttaaaaa tagtgtctat
    65101 ttcgtgagaa aacaactata ttttttatgt gttttgcttt ataattaaaa aaatattgtc
    65161 attttatgga ggaaaatata tggaaaatta tcttagcctt ttaggcgtct tgattgttat
    65221 tctcgggttt gcatttaaat tagactcgat attgatcgta atggttgctt tagttgttac
    65281 agctttgact ggcggtcttg gcattcaagg aatgttagaa cttttaggta caagttttgt
    65341 aaacaatcgc gggatggcga tttttatcat cattatgttg gcaacaggaa cgcttgaaag
    65401 aaatggtctg aaagaatcag cggcttcctt gatcaagcga ttcaagaaag tatctgctgg
    65461 tatgattgta gatatttatg ggatctttcg aatgatcttt gcggctttta acgtgagttt
    65521 tggtggagtt gctggatttg ttcgtccaat cattttaccg atggcattgg ggaccatcga
    65581 atcaaaaggg ttagaagtcc atcctaagca tgaagaagaa ctaaaaggta tggcttcagc
    65641 tatggaaaat gtctgctggt tctttggcca agtactattc atcggaggag caggggcgtt
    65701 gctagtccaa tcaacactga aagatctagg atatgacgta acattgggac agcttgcttt
    65761 agtggaagtt ccagtggcta tcacggcttt agttgtggca agtgtctact tctatcttaa
    65821 agaaaagaaa ttagcgaaga aatattatcc aggaaagggg caataagatg gactttttta
    65881 caaatcctga tattttatta ggcgataaat tactggagat cctttatatc gtgatgggac
    65941 tcgtattgat ttacactgga gtaagaaatt taacagataa aaccaatcct catcgttatg
    66001 ggtcagcctt tttctggacg gtcttagggg tagttattgc aggaggaaga tggcttccgg
    66061 caatcgtgaa cggtggcttg atctttgcga tgaccatacc agcaattgct aagaaagtca
    66121 gcaaaggaga aagccggctt ccatcaaaag aatatatgga aaaaatgtca gacaaactag
    66181 gaatgaaaat ttttattcca gctttgagta tcggtgtctt tgcgatcttg tttgctctat
    66241 ttaccaattt aggtgcttta gtcggcgtgg gcgtcggtgt ttttgcagcc atgctaatct
    66301 tgatgttctt ttctcgagat aatcgaccaa gtacattttt agatgatgca gcagatatgc
    66361 tgggcacagt agggccgtta agcatgttgc caatgctttt agcttctcta ggtgcggtat
    66421 ttacaagcgc tggagttggg acagtgattt ccaatgcagt aggtacgatc gttccagaag
    66481 gaaatgtgaa catcggcatc atcatttacg ctcttggcat ggtgatattt acggtaatca
    66541 tggggaatgc atttgcggcc atcaccgtga tgacggttgg gatcggtgca ccattcgtat
    66601 ttagtcatgg agcgaatcca gcgttaatag gaatggttgc attgacttgc ggtttttgtg
    66661 ggaccctgct gacaccgatg gctgctaact tcaatatcgt gcctgtagcg atgctggaga
    66721 tgaaagacaa atacggagtg atcaaaaacc agttgtttat tgcgttattt atgctagttt
    66781 tccaaattgc ttatatgatt ctatttaaat aatagaagag aggggttgtg acagaataat
    66841 tgtcgcaacc tcttctaaca ttgtagtgga acactatttt tttataagaa tatattaagt
    66901 ttgatctgtt atgttattta aactattgtt tcctttattt acgctgttca aggaaaatgg
    66961 tatgataaaa gataaccata tgttaaatca atcggacgga ggaacaacag atgaaagtac
    67021 tagtaacagg ttttgatcca tttggtgggg acaaagtgaa tccagcctat gaagcagtaa
    67081 aaaaaatgcc agatgaaatc agcggtgctg aaatcatcaa agtggaagta ccgaccgtat
    67141 tcgaaaaaag cagtcaagta gtcaaagaag ccatccaaca gcaccaacca gatgtcgtga
    67201 tctgtgtcgg acaagcaggt gggcggtcag cagtctcgtt cgaacgtgtc gcaatcaatc
    67261 tagcagaagc aagaatccct gataatgaag gaaatcagcc attcgatacg gctttagaag
    67321 aaaacggtcc agccgcttac tttacctcat tgccaatcaa agcaatgaca aaaaatgtac
    67381 aagatcatgg tctaccagcg tacatctctt atacagcagg aacatttgtc tgcaatgata
    67441 tcatgtatcg tttgcttcat ttgatcgaaa cacaattccc ggcaattcga ggtgggttca
    67501 tccacgtgcc attctcaccc gatcaagtga tcgatcgtcc agtgggtacg ccttctatgt
    67561 ctttagaaga cattgcttct tctttgactt atgcagttga agcagcagta gaaaataaag
    67621 aagatatttc cggaaatgcc ggaacaactc actaatttag caagaaacac tggctggttg
    67681 ctaacgttat gttagtagcc agctttttgt aattagaaag gaaagaaaca gccgtggaag
    67741 aatggcaatc cgtctttgaa gaatggtttc caaaagaaat cagcaaaagc tatcccatca
    67801 aaatttctaa acaatacaca agcagtcaac gttgggagat ctatgcaaaa ttaacgaaaa
    67861 aacagcggga actagtcgat aagcaccgcc gatatctaat cagctctcgt tttatggaag
    67921 aacattattt agctgcaaca gattgggtgt tttctgactt caagatcaat ccgttttttc
    67981 gaacaaaacg aagccagcaa aaattgtatt gtgagtgtgg tagagaatta aaagtgcaat
    68041 acatcgttaa atcgccaaaa accggaaaga tcctaaaact aggcatcaac cactttgccg
    68101 atcacctgca tgtctctccg acagtggcag cttcgatcca tcagggaatg acaaaagttg
    68161 atctggcctt agacgaatta ttatggctta agcagaagaa tatcgatttt cctgaaggac
    68221 tatggcaaaa atactgtttt gtcttgtacc aaaatcgtcg gatgaaacag ccatatttac
    68281 cggatataaa attagctcag cggctcgcag aatttcgcca agtagaaatg ccgatttaca
    68341 tagcggatta tcaagcattg gaaaatgaaa tcaagaaaat aagtgaacac atcaatgggc
    68401 agtcaaaaaa gcgacagata aaaaaagaac tatttgacga ctttgcagaa gaattagtaa
    68461 aggatgtaga agaattcttg atcaactatc gcgcattttt acgtaaagat tggcaatcca
    68521 tcgtatatga agaagtgcca gtacatccta atgcttattt tgaaacattt atttccgtgt
    68581 tgagaaagac aaaacgtcaa cgaacaccag aagtcactgc tcagatggaa tattttgcga
    68641 aaaaccaacg attcatccaa ccaaaaatct atctatttat ttggaagcaa tattgtcgct
    68701 atggttttac agaagggttc ttcgacagca ttcctcggat agtcagaaat ggttttttaa
    68761 aagtattgcg taaagaacga gaagctatac aatctgctga taagaaagac cgtacagtct
    68821 caaaagaaaa atggcaacta gtagtgaaag atatccaatc tggaaatgtg caagagacaa
    68881 tcgacaaatg gaaaggaaaa cattatcgtt ttactgaagc tcagaaacaa gcgttggaat
    68941 attatcagaa gttagaagaa tcactgcgtt tcaatgatga ggcaagaaag tatctgaagg
    69001 aattactata aaaaatagag gctgggacag aagcgtttaa ctccaagaaa taagaagaaa
    69061 ttcacgaaaa ttgcttttca aatttttgtg aatttcagct tatttccgaa ggagttgctt
    69121 ctgattccgc cgtttatacg tgtttagagc gtgagacaaa agtgttcttt acttttgtcc
    69181 cacgctcagt ttttttatgc gtgttgttgt gcgttgtagt attcttcttc cattttttgc
    69241 atacgttcac gttctttttg gttataccaa ggtgaaacgg taaatgcaag gacatcattc
    69301 agcaagtaaa cagagctgtt cacgaacatc gccaaacttg cagatccatg cgtaaatgaa
    69361 atataccaaa ggaccatttg agcaagccct gaagctaacc accagtaata ttggttgttg
    69421 taacgcaaga aactgatgat accagcagtc aagctaatag aaaaagcaat ggcatctacc
    69481 cacggccgag gatcatctgt ataacgacca atcaggaatc ctgaaacaag atagacaagg
    69541 aaagtactga tgattgcgat aaaccagctt tttccagtga attttcggat ttttgaagcc
    69601 atattatgat tccattcttt acttaacaat acggggatat ccaatgtgat catataagca
    69661 atctgttcac cgatacttaa atagttcttt gctgaaaacc caacgataat aaaacaaatc
    69721 gcagacaaaa tacctaaaat accgttgact gattttgccg cattgatgga taaaatacac
    69781 aatacaccaa gcgtggtacc gataaatgta ataacggaca gccaagtgat gggactattg
    69841 acaagagtca tgacctggca gccaagactg aaaaagaaaa gataataatt ttgttgcggc
    69901 cagcctttta gctgatgagc taaaaactga aaatatgact tcatgtatga ggactcctta
    69961 agacactaaa tgtagtgtgt tctgtattta ctaacacaat ctattgtatg cttttcttga
    70021 aaaaaaacta gtctttcaat tataaataat ttgtgttatg ataattttct gtttgttttc
    70081 ttgaaaatat aatttcttac tttcatttat tatattggga acaggaagag tgtcttggag
    70141 gacctacttt ctgttttttt tgcctttttt cttataaaaa ggaaaaacag ctattttttt
    70201 caatctgaca catttacaat ggaactggag attaatatag atccgagctt aaacgaagaa
    70261 taggagtgct catatgaacc ttacaacgat ccatcatata gccatcattg tttcagatta
    70321 tcagaaatca cgtgattttt atgttaataa attaggattt gaagtaatca gagaaaatta
    70381 ccgcaaagaa cgagatgact ataaattaga tctgaaacta ggaagtgctg aattggagat
    70441 ttttggaatt gctaatgcgc cacaacgacc aaactatcca gaagcttgtg gcttgcgtca
    70501 tcttgctttc aaagtagacg atattgaaga aacaattgca gaattgcagt ccttagggat
    70561 cgaatcagaa ccgattagag aagatacctt tacaggaaaa aaaatgacgt ttttctttga
    70621 tccagacgga ttgccattag aactgcacga ataaagataa caaaattgtc actttttttt
    70681 cgtttcattc gatttttcat tcgttcattt cttgatattc tttaaaaaaa agaaacagga
    70741 gaatgaagaa tgaaaaaaat gatgtttggt gttgctgccc tcatcgtaat agggattacc
    70801 ggtatgacgg gactgctcaa tgccgctttt cacatggatg atattagtga ccaggaatat
    70861 cggatctcat tgaaaaaagc agcagcaatc gtaaaaaacg aaacaaaaga acataacata
    70921 tctgctattc attttgcagt tgaaaaagat ttggagaatc ctacgaatgc aacatatgaa
    70981 taccagtttt tttgtgaagg acaagtcata gcagtcaatc catccactgg gaaaacaacc
    71041 gtacgaaatg acacggaaaa ggccgcagaa catctttttg atatagaaaa aacggaagag
    71101 ttcaaacaac cgcaagcagc gatgaaggaa gcagtcagct actacgggaa aaaagcaaaa
    71161 gtgaaagagt ggacacttct agtcaaagat gaacgtcctt attataaagt ccgattaata
    71221 gatggagaac aggaccgtca ggtatggata tctgcataga agaaggatat tgcttctctt
    71281 ttttacaata taagagtaag ctaagagagt aaagatgaaa ggaagttgag ataacatgga
    71341 aaaaatgaaa tgccctaatt gcggaaaaaa atttgcatac gaagaagtca ataatgtagt
    71401 cgaacataat gataaagaaa tgccagtcgt ctgcccttat tgccgaactg aagcggcaag
    71461 aatcgttact catggttatt tcattactga aaagattgaa gattttttaa aataacgatt
    71521 ttgcgatttc tttttctgca tgttagaatg gagaaaagga gaggaaacta tgattcgttt
    71581 tgcgagaaaa gaagatgctc cagaaatcgc cccattgatc ttagtgattc taaaagatat
    71641 ggaactgcca tttttgaagc agacagggga acaaaagact ttagagattt tagaagaagc
    71701 aatcactgat ccaaattatc gttacagtta ttcacgaggt atcgtagaag aaatagacgg
    71761 taaagttgca ggagttgctt acggttatac agatgaagaa gaatcaatca tcgatcagcc
    71821 actggtacct atcttagaga aatatcagct agatgcgtct acacgtttgt tcattgataa
    71881 agaaacgttt cccaacgaat ggtatttgga ctctatcagt gtatcagaag attttagagg
    71941 acaaggcatc ggttcgcgat tattagaagc attacctaaa ttggcgaaga aagctaatcg
    72001 ctccgtcatc ggtctaagtg tggatgaaaa aaatccaaag gctaaaaaat tatacgaacg
    72061 ccatgacttc aaagttgtcg gtcagcggat gatcagtggt catctttatg atcatatgca
    72121 aaagaatatc taaaaagatg tacctgaaat gcgtatttcc gcatttcagg tacatctttt
    72181 taacttaagt taagtcggta agaaacaggc ttatcgcgac ttttgtcttt acggtagagc
    72241 cagtaatcta aaaccgcagc aatacaaata caaactggag catcttcatc atttgagaca
    72301 tccaatacat agtaatctcc tgaaaaaagt gttgctttat ccattttcat gatttgctga
    72361 ttgaaatgat ggatttgata ttgatgatta tagatatcac cttggactgt ccagtgaagt
    72421 ctacgaatat aataaaagtc accgggccaa ttgaatattt tctgcatggt tcccaccttt
    72481 tcgaactctt tatacagctc aaaacgcgta ccgaatgtca ggcttatctg cttgatgtga
    72541 gcaacgagat cgccattcat ggcataaaga gacaatgcat cgccctttgt tccccagcga
    72601 ccaaccatca aaaacaggga atgtcctttt tcgtttttga caattgtccg agttgcacgt
    72661 ccaagctgct tttcctgaat gaaaaattct gacatctata tccctctttt ctagataaaa
    72721 ctacaagtct tctgcaatcg cgcgcaatat ggcttttcct acgccagatt caaggtttgt
    72781 gtccgttgta tatttggcat aatcttttac ttcgatttct gcatttccca ttgcgaaact
    72841 gactcctgct acttgcagca tgctgacatc attgaagtta tcgccaatcg tcatgacttc
    72901 ttctaaagaa atcccacgct cgtgagcgac atgggcaaca gcaattcctt tttgagcgtt
    72961 tttatggttc acttcgatat tgttttgacc agaggaagta acggctagat cgcctaaaag
    73021 agaaatctgc tttccaactg gacctaatac agctggccct tctgtatgaa aacaaataat
    73081 ttttaaaact tccagattct tttcttccaa aatagcacga atactgtcca cgtatgtgat
    73141 gtgcaataac tccagcttag ctgaggccat ggcaatcgcc attttatacg taaggtgagg
    73201 taaatgggta gccaccatgg aagcaaaact ttcaattcgt ttcgcttgat tttcagaata
    73261 aagaccatcg ttggttgcta cttcatagta aataccgtta gcatctagaa tatctaaaac
    73321 agagattgct tgattatttg gaatcgacac ggtaaacaat gaatttcctt ctttatcaaa
    73381 tacctgtgcc ccgttcaaag tgatcatcgc acaatcgatt cctacctcat ctaaagctgc
    73441 gcgagcttct tgatagtttc gaccagtagc gaccatgaac tcaacaccgg catcatttgc
    73501 aaaacgtatc gcctcggcat tttcaggaga aacactcata tgggcatcta ataatgtgcc
    73561 gtccatatct gatgcaatta atttgatcat aatgattatc gttcctttct gcttcttgtt
    73621 cctagtttac catattaatc aagtgctgag ggtgaagatt gtcccttgtg attgaagaaa
    73681 atgtgagtta ttctttttat taggaggtgg agtttttgaa agaaatcatt cataacagtt
    73741 ggcaagaagt tttgtcctca gaattttcaa aagattatta tctgcatcta agagaatttt
    73801 taaaaaaaga atatgcgtcc caaaagatcc atccagatat gtatcatatc tatgaagcat
    73861 tggaattgac tccttatgaa gaagttaaag tggtgatttt aggacaagat ccttatcatg
    73921 gagaaaatca agctcatggc ttatcttttt ctgtgcaacc tggcgtaaag atcccgccat
    73981 cgttaagaaa tatctataaa gagctttatg atgatcttgg aatcgcacct gttcaacatg
    74041 gaaatctagt gtcatgggct aaacagggag tccttttact caacaccgtt cttaccgttc
    74101 gggaagggca agcgtattct catcgcggga aaggttggga aagattaaca gacacgatca
    74161 ttgaaaaatt aaatgaacgg gaaaaaccaa tcgtgtttat cttgtgggga aaaccggcac
    74221 aagaaaaaat caaaatgatc gataagagtc gccatatcat cataacatct ccacatccca
    74281 gtccgttatc tgcaagtcgt ggcttttttg gttccaaacc cttctcgaaa acgaatgatg
    74341 cgctccttgc gttaggagaa gaaccgattg attggcaact gcctgaaaca gtgtaataaa
    74401 acagcgcttt cctgttatag tgcagttcgc tttgtatgtg aaaaacttaa tttttccagc
    74461 atttttgact gatacagata aaataaactg ttcttttttt ctggaatagt gtatgataat
    74521 acatataaaa acgtctggag gtcatcttgt ggaattgttt gatagtttaa gatttaaaat
    74581 tgttcgccgt gggatcaaaa tcgttttccc ggaagctact gatgctcgta ttttaggcgc
    74641 agctgcccgg ttgaaagcag aagaacttgt ggaacccatt ttagtaggga acgaagaaga
    74701 aataagaaat gcagcgcatg ccagaggaat caaaacatct agctttacga tcatcagccc
    74761 tgataattac gataagtggg atgaaatggt ggatactttt gttgaacgta gaaatggaaa
    74821 ggcatccaaa gaacaagcag aaaagatttt aaaagatgtg aattactttg gtacgatgct
    74881 tacgtatatg gggattgcag acggaatggt ctctggagcg atccattcga ctggcgacac
    74941 tgttcgccca gcacttcaga tcatcaaaac aaagcctggc atcagtcgga caagcggagc
    75001 gatgatcatg gtacgtggaa gagatcaaga aaaatatgtc ttttcggatt gtgcgatcaa
    75061 cgtgaatcca actgcgcaag aattggctga aatcgccgta gattctgcaa aaacagcaga
    75121 attgttcgat atcgaaccaa aagtagcaat gatgagcttc tcgacaaaag gctcagcgaa
    75181 atcgccagaa gtagacaaag tagttgaagc aactaaaatc gctaaaaaac tagcgccaca
    75241 atacatgatc gacggcgaat tgcaatttga tgcttcttat gttccttcag ttgcacaact
    75301 gaaagcgcca gattcagaag tagctggtca agcaacagtc tttgtatttc cagaattgca
    75361 gtcaggaaat atcggctata aaattgctca gcgtttcggt aactttgaag caatcggacc
    75421 aattctacaa ggcttgaaca aaccggtttc tgatttgtct cgtggagcaa atgaagaaga
    75481 tgtatataaa ttatcgatta tcacagcagc acaaacattg attgattaac aaatacacgc
    75541 gaactagcca aaaacagcag ctgctgttct tgactagttc attttttctt taaaaaaact
    75601 tcattacgcg atatactggc aaaaagaaag aagagcggga gaagatgaaa aatgatcgaa
    75661 ctatcaggat taacaatgac agaacaattc gcagaggcaa ttggtcattc cgcaatgcca
    75721 ggtgacaatc tgatattgac tggtgatcta ggtgcgggta aaacaacgtt gacgaaagga
    75781 attgcccaag gattagggat aacgcaaatg atcaaaagtc cgacatacac gattattaga
    75841 gaatatagtc aaggtcggat tccgttgtat catatggata tttatcgggt agcagctagc
    75901 ggagcagatt tagggttaga tgaatatttt gaaggtgatg ggctttccgt tgttgaatgg
    75961 ggaaatctat tagaagaagc actgccggag gattatttgg aactgatttt agaaaaaagt
    76021 gatacagatt tagaaaagcg gtatgtgaaa ctaatctcat acggggagca aagtgaaacc
    76081 ttcaaacagc gaattctaga aaaatgggct gaaaatcatg agtgaagaag ttgggatcat
    76141 cttgcgggaa gcagtgccag gagatgcaaa agacattctt ttgatgatgg gacaagttaa
    76201 taaggagaca gaatttttag ttttggatga agcagaactc ctgttaccac cagaaacctt
    76261 agaagaagag ttggattata tatacgaaag caataataat ttattgctgc ttgcaatcta
    76321 tgaagggacg attattggaa cagcatctgt caaggcagac agtcaatttc gtttgtctca
    76381 cgtgggagaa gtcggtatca gtattctgca ggaatattgg ggaatgggac taggcactct
    76441 tatgctagaa gaaatcatca gttgggcgaa ggaaatgggt gtactttttc gattggagct
    76501 tgacgttcaa gttcgtaatg aaagagccgt acacctttac cgaaaaatgg gcttccaaat
    76561 cgaagcggtc atgcctagag gggcaagaac agatctaggt gaatttttag atgtatataa
    76621 aatgagttat ttgattgaat agaagaaata ggaaaagagt gagaaactgt catccttttg
    76681 agaaggcaga aaaacacacg aagggactgt gacaaaagtc gtgtcgcagt ccattttttt
    76741 tgaataaacg gtgttcaatc agctgacctt cggtattagc tcaaaaaacg gcaaaatatg
    76801 aggaactatt ttgccgtttg tttctctaat actcaggtca aaacgctgat ttcacaacct
    76861 cttggtaaac ggtgtccaaa agcttgcatc tgcggtaata agtccaacca aatgaaaaat
    76921 atgagaagct atttttgttt ggctgttctt attacttgat gcagaacagc tttttcacaa
    76981 cctctccaat aaacggtgtt ctcctgccag ctcctcggtc accagtcaca atatttaata
    77041 aacatgagaa gctatttttt gaaattgctc cttgtgactc ggagtgaaac ggcaggctca
    77101 cgacctcttt gtgtgtttca taagtattct tttactttat agtcacaagt tttttcaatg
    77161 gatctgtccc gaattttttt tcttgataaa gcaggatttc cgcacatgca cgactatctt
    77221 ccaatgcatc atggtgatga tctaattgga tgttcaaatt ttcgcacaca gtgttcaatc
    77281 gatgatttgg aaattcagga aacaggcgac ggctactttt cactgtacat aaagaaagat
    77341 aattaggctg ttcaagatga taataatcta agcatcccgc taagaccttt gtatcaaaag
    77401 cagcattatg tgcaacgact aaacgatttt tttgaaaata tggttggata gtttgccaga
    77461 cttctgggaa tttcggcgca ttgcgtacgt cttcttgatg gattccgtga atttggacat
    77521 ttcgccagaa aaaaggagtc tcaggctgta tcaatgtata gaattcatca acgatctggc
    77581 tattttttac catgactagc gcaagagaac aagcactata aggctgatga ttggcagttt
    77641 caaaatccat ggcaacaaaa ttcatttctg taaaacctcc ttgggaaatt actgtaacat
    77701 acttttgaaa aaagcaaata aatttaacaa aaaacataga attttttatg taatatttgt
    77761 tgagaaaagc tagtttttga actcattcct tgtgatttgg agttagatgg ggatcatgac
    77821 ttctcggtat taagtcaaat ttatgcaaag ttgtgaggaa ccattttcaa aaaaatttct
    77881 tacgactcga aagtaggcga ctttttcaca acttctccac taaacggtgt tcaatcagct
    77941 gaccctcggt attagctcaa aaaatagcaa aatatgagga actattttgc catttgtttc
    78001 tctaatactc aggtcaggac gctgatttca caacttctca ataaacggtg ttcaaaagct
    78061 tgcatctgcg gtaataagtc caaccaaacg aaaaatatga ggaactattt ttgtttggct
    78121 gttcttatta cttgatgcag tgcagctttt tcacaacctc ttaataaacg gtgttcaaaa
    78181 gcttgcatct gcggtaataa gcccaaccaa acgaaaaata tgaggaacta tttttgtttg
    78241 gctgttctta ttacttgatg cagtgcagct ttttcacaac ctcttaataa acggtgttca
    78301 aaagcttgta tctgcggtaa taagcccaac caaacgaaaa atatgaggaa ctatttttgt
    78361 ttggctgttc ttattacttg atgcaggaca gctttttcac aatctctcga taaacagtgt
    78421 tcaaaagcca gtttctcggt attatgccaa atttatgcaa aatgtggaaa gccattttca
    78481 caaaattttt cttaatactc gaaactaaac gactttttca caacctcttt atttcaaatc
    78541 taattctact ggacaatgat cactgcccat aatgtccgta tggatctttg catcagctag
    78601 tcctggctct aaatctttgc tcgtcaaaaa gtaatcgatc cgccaaccag cattgttttt
    78661 ccttgaattg aatcggtagt tccaccaaga gtagacacct tcctgggttg gataaaaata
    78721 acggaatgta tcgataaacc cgttatccaa cagtcgagaa agagccgctc tttcttctgg
    78781 agtgaatcca gcattttttt gattcgtttt ccagtttttc aaatcaatct tttgatgagc
    78841 gacatttaga tccccacaca cgatgactgg tttttgtttt tttagatttt ccaaataatt
    78901 ataaaaggct tcttcccatt ctaatcggta atctaaacgt tttaattcgc tttgagaatt
    78961 tggcgtgtaa caggttacaa gaaagaaatc ggaatattcc aaagtaatca gacggccttc
    79021 tgtatcatgg atatcgatac ccattccgta agtggcattc aatgctggtt ttttagcaaa
    79081 aatcgctgtg ccggaatagc cttttcgttc cgcgtaattc caatattgat aataacctgg
    79141 aagatctaaa tcaatttgtc ctgcttggag tttcgtttcc tgcagacaga aaaaatcggc
    79201 gtctaactca tgaaaaactt ctaaaaaatt tttatttacg atggcacgaa gcccgtttac
    79261 gttccaagag ataaatttca ataaaagcac actccttcga attaatttcc ttacctttgc
    79321 gatactattg tacctcattt tagtaaaaaa tggtgtaaga tgaaagtaac taaatgatcc
    79381 aggaggaaag tagatgaaga ttggtgtaat cggtgtaggg aatatcgcag aaaaagcata
    79441 tttaccaact tatgcgaaaa aacaaggaac acttgatttt tattttgcaa caagaaataa
    79501 agaaacaaaa gaaagaattc aaaaagagta cgggttccaa catctatatg aaacgatcga
    79561 tgagctttta gaagaaaaaa tcgaagcatg tatgatccac gcagcaacaa aagtacatta
    79621 tgaattagcc aaaaaatgtc tagaaaatgg tatccatgtt tatattgaca aaccactgag
    79681 tgtccatcta aatgaagttc gtgaattgca agaattagct gataagaacc aaataatttt
    79741 gatgatcggt ttcaaccggc gctttgctcc tatggtagaa gaattgaaac gtatcccaga
    79801 taagcggttg atccacctgc aaaaaaatcg aatagcggca aaagaaacga cagagttcgt
    79861 cctttatgat ttgtttttac atcttattga tacagctgtg tatttattag atgaacccat
    79921 tttgagaaca aaataccaga taagagaaac aaaggaattt ttggaatatg ccattttaca
    79981 attagaaaca caaaatcaaa cggctatttt aacgatggat ttagcaagtg gcgctaatat
    80041 agaaaaatat caagtaacaa gtaaaaatgg tacttatgaa gtaaacgatc tgacccagct
    80101 tgtcattcaa gaggctgatg gaaaaaaagt gaaagaattt ggcgactgga caaacacgct
    80161 agtcaaacgc gggtttgagc caatggtaga ggccttcttt tctcagatcc gtttaggtaa
    80221 atcggaaaca gaattgctta aacaaaaaga agttgtaaga agtcatgaat tatgtgagga
    80281 agtgcttaaa gatcactacc gtcatcaact ttgaacaaga ctggcaagat tgtattaagc
    80341 gtgatagaat aatgaggacg tttgagaaaa gaaaggaaaa tcctcagtga ataaagaaga
    80401 aatagttgaa agcttagcca cgattactat attgtttgat gaaccattga tgaactatac
    80461 gtttacgaaa acaggtggac cagctgatgt ccttgcattc ccgaaaaaac aagaagaagt
    80521 aaagcaaatc atcgattatt gccgaatcca tgatatacct tggatggtat taggaaatgc
    80581 aagcaatctg atcgttcaag atggcgggat tcgcggtgta gtcatcatgc tgactgaaat
    80641 gaaacagatt catgtcaaag gaacgatggt cattgcggaa gcaggtgcct cactgattga
    80701 tacgacttat gcagctttgg cagaatcatt gactggattt gaatttgctt gcggcatccc
    80761 gggaagtgtc ggtggagcgg tttatatgaa tgcaggtgcg tatggcggag agatcaaaga
    80821 tgtttttgct gaagtggatc ttttgcttga agatggaaca ttgaaaactt tgacaaaaga
    80881 cgaaatggta ttttcttatc gtcacagtaa agtccaagaa ttacgtgcta tcgtattgga
    80941 agctcgattc agtttgcaaa caggggatta tgaagcaatc aaagcaagaa tggatgagtt
    81001 gaccgaattg cgtcaatcaa aacagccatt agaataccct tcatgcggaa gtgtctttaa
    81061 gcgcccagta ggacactata caggtcagct gatccaacaa gcaggattgc aaggcctgaa
    81121 atggggcggt gcacaagtct ctgaaaaaca tgctggattc atcgtcaata tcgatcatgc
    81181 gacagcgaca gattatattg aattgattgc gcacatccaa gaagtaatca aagaaaaatt
    81241 tgatgtgtct ttagaaacag aagtgcggat cattggagaa aaacaagcaa tatcagaaac
    81301 aagcagaagt ttagccaatt aataagtaat ggaacttaaa ataagtaaaa gagcaggacg
    81361 gtggccgtcc tgctcttttt tgtggttgtt tacgcaaatg gcgcaaatct tgtacatttt
    81421 aacacaataa aacaaaaaat atagaatatt tcctcataaa tttttccatt ttttgcaata
    81481 ttctcaaaaa aaagataaaa aatgagaata actgatgaaa aaacaagcat aatccatcat
    81541 aaaatcagta taggtgaaaa gatgaacttt catctgcata tcaagtaatt ggtatgaggc
    81601 attttttgag gtgtaggata tgaagttgga ttaccttata gaagaggaag atagagaaaa
    81661 actgctgcta gctagatatt tagaatttga gaaaaagaat tatgtagtgc tgcctaagtt
    81721 atatgaaaca ttaggtttgt ctaaatttaa aacaagaaat tatatcatag aattaaataa
    81781 agaatttact gaattcaaaa caaagccaga gatccaaatt tcagaagaag cagagatcca
    81841 tgtcgaacgt ctgaatttga cggatatcaa aaatttaaga agaatctact ttcaacgatc
    81901 aaaaattgct cgactgttaa cggagatcgt ggattcaaaa gtaacgatcg aaaaatttgc
    81961 agagaatcat tttttaagca tatcaagagc ctatgtaaaa agaagagaat tagtctcttt
    82021 tcttgaacag catcaaataa agttaaagaa aaacatatta gttggggaag aaataaacat
    82081 acggaacgta ttatttgggg tatatttcgg ggtctttaat ggtttttcta tgccatttag
    82141 agaaagcgtc atgaatgaag caaagagtat cgtgcaatat tttgaatatc tgttcaattt
    82201 aaaactgtct gcaacaaaac gaatcaaatt caatttgcta accactatta gtttgttgag
    82261 ggcctatggg aatcaaccaa tcggaaaacg tttcttttca gatgaatact ttaagagctc
    82321 agaaggaatg aagatcatca aggatttatc acatatttct tttcttcata tccctaaaga
    82381 gcagatcatt gatgagattt cttatatcat gttgtttttg atcacggaag aagaatttga
    82441 attagatggc gcatttggac ttgcggaagc tcattttcat gatatccaca tgattagtca
    82501 aaaaatgtca gtggaattga ttgatcaatt accattttcg attgaaatct ctgaagaaga
    82561 aagactatat atacgaaaag aacttcgtat gggtatgatg catgtcaatc gaaaaaattg
    82621 gatattcgac tttgagatga ctagctttac gtctaaaaag cagctacagt ttttcttcga
    82681 gacgtatcca atattcagta cagcaatcca aaaggtcata caagagaata aacgctattt
    82741 cttgggaaaa gatgaacgtt tcatgattcg cttattttat gattatctct ttttattggt
    82801 cggtatttgt ccggtatcat attttgaagc accgattcat gtctgtattg atttttcgca
    82861 aggatctgct tataccgatt acattatttc tcaagtgaaa ggatttaaaa attacaatat
    82921 tgtgattgag agtcgaatca cttctcgtac gaatatttat ttatctgatt gtttacttga
    82981 aaaatttaat aaagaacaga taatttggaa aaatccaccg actccagatg actgggaata
    83041 ttttggaaat accatcatca gagaaaaaaa tagacattta actgaaaaaa tcagctagta
    83101 tttatggatg gcagctttgt ctcacatatt tattagctga tttatgtttt tttcaaagaa
    83161 aaggtaacta gatataacta gatataacta gatatattac ttaacaaaat taaaaagtac
    83221 ataagttatt attccgattt tcttgaaaca ataaaaaatc aaaaaaaata ctctcctaat
    83281 agtccatctt cttttgattt tatttatata atgaaatcat cttatgaaaa ttactacttc
    83341 atgtttagac aagagtaggg aggactaaga ataaatgtgt tcaaaaagac gttcaaaaca
    83401 actaaataaa cacgcttgtc gtttagataa ggaacggtcg tgttctgaaa aacactacgt
    83461 taagtttatg atgcgcacga tgctgatcag tttatctgta ggtgtctata tgcatgtacc
    83521 cgttcaggca acgacacaaa ccatcaaggt cgtacgtacc tctgtttctg agaccactga
    83581 ttcccctatc actattactc atactttcac tgatgcaaat ggaaaacagc tacttcctgc
    83641 attgacttcc tataataaag tcagttatcc tccaacgata aaccactact ggttaattcc
    83701 taacaaaaca ttatggacat cggaaaccgg aagcagtgtg ccgctttcta tcttttccga
    83761 tatgactgat cctacaaaag gtgatcagtt aaaacagatc actgatattt tgaatcatgc
    83821 tttgacagat ataaaggacg gtggaagcgc acaagtttct tatgtatatg aaaaagatta
    83881 ttcacagttt gaaagaaaga aaatcgatgt tctggcaggg acaaaagttt cgatatctga
    83941 tctggtagaa aggctgaaga acgcagatgg aacacaagga gatatgtcca aagtaatttc
    84001 aaacaatggg aatatcgtgg atacgactaa tacaggaaca tctactattt ccttgtccta
    84061 ttttgatcca attgctttaa aaacaatgac tgcatctgct gtgatccatg ttcgtgctga
    84121 tcaaacagct atcaaaggaa aagatcttca cttaagaaca ggagagagtt acgcccctaa
    84181 agatcttgtt gaatatgtaa caaaaagcga tggaacttca ggaaataaag aagaaatcaa
    84241 aatgaacggg acagtagata ctacaactcc gggagaatat cccgttgtat taacttatat
    84301 agactcgaaa acatcaaatg tggcagaaac aacagctgta gtttatgtag aagcatctgc
    84361 tgcttcatcg actgtcatca ctcattttat agatagtcaa acaaataaag aacttcaagc
    84421 agaaacacat atgagtagtg taaatcgtat tcctgatttg cgcctacctg ctgaattttc
    84481 tgatcgagtg ccaaatatag atttatcgac ttatgaacaa aatgggaaag tcacttctat
    84541 ttctgattgg atgcaagcaa acggattaga cagaaaaagt cactggtcgg agatccttga
    84601 agtacttcac tcagaactga tgaataatag gaagcaatca ggaggggaaa ttacctttac
    84661 ttatgtttat ataccagaca atcgttcatt agaaggtcat gatcttattg ttcatccaaa
    84721 cgaaaaaata aacaaagaag agttgatcca atcagcaaca aattccttag gtaaacccgt
    84781 accgttaaag gatgtatcgg ttacaatcaa tgaagtacca ttggaggatg gttatttgac
    84841 caaacaaaca ggtgatttta cgattatgta tagttatcat gacccgtatt cactccaaga
    84901 actccatgct cagtcactat tacatgtttt accattaaac aaacagatag atcaaaaaga
    84961 acaaaaaaga cgagaagaaa gcttgtcaga aaaaaaacaa caaatggctc atgcagtttc
    85021 tccaacaggg aaagtaaaac aaacaagaag cctagagtta gcaagaatat ctagaaaaat
    85081 ctcagaatct aataaaacga taaggcaaga agctcatttg gccccacaag tttctggatt
    85141 ggaaaatgaa attttagagc ctaaaataaa acaacaatca gaaaaagaat ttgttgctaa
    85201 acagtcggaa gatcagaagg aatcagatga ccagaaggaa acgaagaaaa aacgcttgtc
    85261 aactattgat tccaccgatg agcatctgca gccgcaagta ccacttccta gtccgggaac
    85321 acccgcacct ggtggaggta ttccttcttc tgctttagga gatttcatgc gaggtatggc
    85381 aggaacatgg gtatacgcta atcgaaatga atttgacgca gatgactggt tctgatttat
    85441 atctctatct gttatagtca aataagtagc tgagtcatat gttatttggc ataatggaga
    85501 cgaggaggga tagaaaaatg ttagataaaa cgttacctta cgcagaaatc tggatgacaa
    85561 gaccacttca acaaaatgtt ccaatcacgc ctttagcgga aggttttgaa tttcgatttt
    85621 atcaaaaaga agatgaatct gcttgggcgg agattgagac ttccgttggg gaatttcaaa
    85681 cgacagatga agcacttgag tattttcaac aaaactttgc tccttatcct gaagaacttg
    85741 aaagaaggat gttttttgta gagacaaaaa aaggagaaaa agtagcaacc tgtaccgctt
    85801 ggcgaaaaaa atcaagagag caagtgtatc cggtttttca ttggttagca gtgaaaccag
    85861 attaccaagg aaaagggctc gcaaaagcat tgactgccag agtcttgcaa gaatttccta
    85921 ccctccatac ggacggacca atctatcttc acacacagac atggagccat caggcgattg
    85981 ctctttataa aaaaatgggt tttactttta ttgctgaaaa tctagacggc acacctaatc
    86041 cagattatga aaaagttatg caggttttaa gtaaattaga taaataacga aatggtgaaa
    86101 taagtcctca gtattgggat aaaaaacgaa atagtcattc ctcatatctt acttttttga
    86161 gttaatacta tcggataact aatggcatgc catacattca aaaaagaagg ggcagtgaca
    86221 aaagtattgt cactgcccct tctcttgttt tttagcatgt tttatcgttt gattttttta
    86281 ggaataaaat tcttgatgaa cgtaaatttt tggtaatcgt ttcgactata gaggtaacca
    86341 aatacactga aaacaactgc tccaataaaa ttaacaaata aatccttcat agtatccatg
    86401 attcccacgt ctaagtaacc attttggatg attgttttat ctgttttacc atctgcattt
    86461 tgactttcaa tgattgtccg agtgatatct ggaacatgat agacagtgtt tccgtcttta
    86521 cttaactcca cactattgat ttgctggatg atcgtatctt tttgcatatc gaattgcagt
    86581 aatttatctg ctgaaaattc cacaaactcc cacataacac caactgtcat cgaaaaacag
    86641 aaggaaacaa ctgctagtaa taaaggagtc aagttcatgg tttttgtatt tttattgagc
    86701 aaataaacaa gggaaaagcc gacaccccca gctaaaaatc cgctgaatgc atgtagaaaa
    86761 gtatcccaca ttgaaaaata gccataaaaa tcacttaact ctcctaaaac tgttgaacta
    86821 aaaataaaag ataacaaaat gatttctagc atatttggaa tctgtatctt taatatcttt
    86881 tccagaaaat aagggatttc aaataaaatt aatgtcagta ataaaagcat tgtatttccc
    86941 cagtgttggt tccagacctg atccactagc ataataacga cgatgatacg taataaaata
    87001 tagattccgt ttgtgattct acctgcagtt tttgtactgt tcgtaaaaaa ggttttcaaa
    87061 tgattcataa aataactcct tcatgtatat ccttaattat actaagaagc gtgtgtttac
    87121 caaagctata tgacttaaag aaaaacagag ttgatattgt gaaaaaaatc acttaatata
    87181 gtataaggag aaaaggggga tacaaatgaa aaaattagtt gctggtttat taacagccgt
    87241 tgcacttctt gctatgagcg catgcggcgc agatcagaaa aatacagcgc agacaagttc
    87301 tagtacgaag gaatctgccg aagtatcatc aggcgcttct gaacaagtat atacagatcc
    87361 aaaagaaatg aaagaatctt atgatgtcgt gattattgga gctggtggtg cagggatggc
    87421 agcagctatc gaagcaaaag atgctgggtt gaatccagta attttggaaa agatgcctgt
    87481 tgccggagga aacacatcta aatcatcaag cggtatgaat gcttcagaaa caaaatttca
    87541 aaaagaagaa ggaatcaaag actcgaatga tgccttctat gaagaaacgc taaaaggtgg
    87601 tggaggaacg aatgataaag aaatgcttcg atactttgtc gatcactctg ctgatgcggt
    87661 cgattggcta gatcaaaacg gtatcaaact ggacaactta acgatcactg gagggatgag
    87721 tgaaaagagg acacatcgtc cgtctgatgg ctcggctatt ggtggatact tagtggaggg
    87781 gttattgaat aacgtccatg agcgggaaat ccctattttt gtcaacgcag atgtgacaga
    87841 cattaagaaa aatggggaaa cggtcaatga agtgactgta catgttcaag gagaagaacc
    87901 aaaagaggtc accggaaaag caatcgtagt taccacaggc ggttttggtg ccagcaaaga
    87961 atttattgaa aaatatcgtc ccgatttagc tgattatatt acgacaaatc aagaaggatc
    88021 aactggagat ggtatcacca tgattgaaaa attaggtgga caaaccgttg atatggataa
    88081 aatacaaatt catccaactg tgcagcagga tgagggggtc ttgattggtg aggttgttcg
    88141 cggagaagga gcaatcttgg tcgatcaaga ggggaaaaga tttgtcaatg aaatggatac
    88201 aagggataaa gtatctgcag caattacagc attgcctgaa aaatcagctt atttgatttt
    88261 tgaccaaggc gtgcgtgatc gtgccacagc tattgaattt tatgaccaaa aaggatatgt
    88321 cacaactgga aagacaatgg aagaattagc agaaaaaatc gatttaccaa aagaaacact
    88381 agcaaaaaca gttgatggat ggaatacgga tgttgcggag aaaaaagatg ctcaattcaa
    88441 ccgtgccaca gccatggaac atgacttatc gtctccgaat tattatgcga tcaaaatcgc
    88501 accaggaatc cattacacga tgggcggtgt gaaaatcaat acgaacactg aagtattaga
    88561 taaagaaaac cagccaatta gggggttata tgctgctggt gaagtcacag gtggcttaca
    88621 tggaaacaac cggatcggtg gaaattcagt tgcggagatc attatttttg ggcgacaagc
    88681 gggcatacaa gcagctcgat ttgcttcgga aaatgaatga aatagaacgt aaataaaaag
    88741 aggttgtgaa atcagcgttt tgagctaata ccgagggtca gctgattgaa cactgtttag
    88801 tgaagaggtt gtgaaaaagc tgccctgcat caagtaataa gaacagccaa gtaaaaacag
    88861 cttcttatgt ttttaacttg gttggactta ttaccgcaga tgcaagcttt tgaacaccgt
    88921 ttattcggaa aaagagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca
    88981 cgtataaacg gcggaagcag aagaaattcc ttcggaaata agctgaaatt cacaaaaatt
    89041 tgaaaagcaa ttttcgtgaa tttcttctta tttcttggag ttaaacgctt ctgtcccagc
    89101 ctcttttttg atcagcatga ttttattcag ttatcttcaa aatgattttt ccgatgtttt
    89161 tgtttgcttc catatattca tgcgcttgtt tgactgcgct gaattggaat gtacggtcaa
    89221 tgattggccg aatcttgcca ttttctagaa aaggcccagc tttttgcata aaagtttgtg
    89281 cgagctctgc tttatactga tcacttctag gtgtcaataa tgtaccaagc agttggacac
    89341 gtttcccaat caatacacca aggtctatct caggcacgat ggtcccacct agcataccaa
    89401 tcaagaccca acgtccatca acggctatag aagctaaatt cttttcccag taagaagcgc
    89461 cgatgaaatc caaaataaca tcgactcccc gaccttcagt gatttttcgt acctcttctt
    89521 gaaagtcttg ttttttatag ttgatcaatt catctgcacc aagttcttta caaaaggcga
    89581 gtttttcttc tgatcctgca gtaacgatga ttttcgcgtg agaaagctgt ctagctaatt
    89641 ggatcgctgc tgttcccaca ccacttgctc ctgcatggat caagacagtt tcgccttctt
    89701 gtaattttcc aagccagtaa agtgtttgat aagcagtcaa aaaaacttct gagattccag
    89761 cagcagaaac atagtctagc gtatcaggga gaaggattgc acgatcggca ggcatcacag
    89821 catattcggc ataaccacct agattgacta atccgtatac ttttgtccca ggagtaaaag
    89881 tggagatatc acttttattc tcaactacct ctccagctac ttcgactccc aaaatagggt
    89941 agggaggttt caaagagact gattctcttt tcataatatc tgttcgattg acagctgctg
    90001 tatggacttt gatcagtaat tcgccttgtt ttggtttagg aacaggcact tcaatttgtt
    90061 ctaatacatc tacagaacca ggatgcttga ttgaaatggc acgcatggat ctattcctcc
    90121 tttgcccatt cttggaatgc tgtattcaac tctttgctac cttcagaaag tactagagtc
    90181 gtacgagaaa tatccgtttc ttccactaga gcggcaagta cagcagcaac tgtagagcga
    90241 gtgatcgttt ttgatgcctt ctcattaggc ttagttaatt gaatggaagt aacttcttca
    90301 tcgttagtca atgtgactgg ttgaagaatg acaaaatcta agttagttcg tttcagccat
    90361 tcatctgcat aatgtttagt aatataataa tctgtcatgg actcaggcca ttttgctggt
    90421 tcatctgcat aaacagcact gaccatcaga tagcgagaga catgcgcatt ttcagctgcg
    90481 atcactgttt tgattgcgcc atccaagtct acttgcagaa gattctttcc ttgagagccg
    90541 gcagcaaaaa tcaatacatc gatgcctttg aatgcttcgg tcattttttc cacggaccaa
    90601 gttaaatcaa aagagacata cgaaacattc tcttttttta ctaatgtttg ggaagcaaca
    90661 tctctcaccc ctgcaaaaat ttcatgggaa cttggagcta aatcttttat caggtgtcgg
    90721 ccaatttgtc cgtttgcacc tacaacaaaa attttcattt ggatcatcct ttcgacgtca
    90781 aatctttcca ttcaatagta acaccaaaag agaaaaaaat cagagatcgg ctacgatgac
    90841 tatcagttag taagagaagg gtgcttgaag tatcttaaca agacattgca tactgctgca
    90901 gtcgcaagac ctgcgacgat tccttcaggt ataccacttg taaaaataac cgctagaata
    90961 gctgaataaa cagcatttaa atttgttccg atgactttgg cataatcttg ctggaataaa
    91021 aagtagatca tattcatcac taaaatcgta ttggtcaatg aaccggaaag tccagctaag
    91081 aataaagaaa cggaatctgc tttttgtttc attagtttgc gcagaccttt ataaacgaaa
    91141 taaggaacca ccccaatcaa gatgcgagga ataaacgcga tcagcaaggc tttccagctt
    91201 ccatgttctg ttccgatgac tgggataaac ggagaaaaga caaaagacaa aggtgtttgg
    91261 ataactgtgc tacggatcat gctgatcagt ccaaatgtac cgccaagaaa agcacctagt
    91321 cttggtccta aaacgatcga agcaatgatt acaggaatat gcatggttgt tgcattgatt
    91381 ggaccgatag ggataaaacc taaaggcgta acagctaata aaatcaaaat agctaaaaac
    91441 atcgcagtta atgtaaagtt ttttgtattt ttcataagta ctcctttttt ctaagcattt
    91501 ctttcattaa gagtcagcat gacctgttcg atgatcgtct gaatatcagc taaggcacct
    91561 tttccaaaat ctccacaagc taaaagagat tctcttggtt caatttcctg atagccgaca
    91621 gatctcaatg tttccagatt acgctgagtg atcgggtttt gatacatatt cgtgttcatg
    91681 gcaggggcaa tcaattttgg tgttcccact ggaagcgcca ttgcgactgt tgaaagaaga
    91741 tcgtctgcaa tgccgtttgc tagcttgccg ataatgtttg ctgtagcagg cgcgatgaga
    91801 aacaagtcag cttgttttgc tagttcgata tgattgatta cctgcggatt tgattcttgc
    91861 atgacgtctg tatggactgc gcgcttcgac agagattgca aggttaatgg cgtgataaaa
    91921 tgtgtgctgc ttttagacat aatcacatct acctgggcac ctaatttgac gaattgactt
    91981 gtaagatcag cgcttttgta agcagaaata cttccagtaa cgcctagtaa gatctttttc
    92041 attttgattc ctccagtcta attttctcag taatcaatgc agcaatctca gatttacttt
    92101 gagcttcttc cactgttcca tctttagata agagatagcc gtggtgtgtc tcgccatgga
    92161 tttccgtcaa atcatttgcg aaaataaaat cagttttgtt atttatcaga ctattttttg
    92221 cgactgccag aagagattcc tttggtacat ctactaatag tttgaaacca atgatttttg
    92281 cttttggctg ccattcacgt agataagaaa tgatttttgg tgttttcttc aaaacgagca
    92341 caagatgctc ggtgttagaa gagatctttg aaacagtggt atctgtctgt tcattttccg
    92401 caaaccaatt gtccagttct tcttgcgttg aagaagtggg taggtttttt gccagctgtt
    92461 cttctgaaaa actaaaagca ggagtaaaat cactgactgc catactgtgg ataatcgcat
    92521 cgtagtgttc ttttttcgtt agttcttcta attgtaaaaa taagtccagt gcagattcga
    92581 tcgtataaat cgttatattg gagtgtcttt tcggtttcag tgcttgtttc gtagtcacgt
    92641 aatcgattgt ggtatccgga ttggcagcta aataatcagc taatgcttgc cctaatcgtc
    92701 ctgtcgaatg atttgtaata gcacgtactt gatcgatttt ttcagtcgtt cctccagctg
    92761 tgattagaat cttcatttat ggtcacctac atttactagt gttcttcctt ggtgtgttcc
    92821 agcaagtagt ttagatgctg tttcaggcaa ttgatccaga gtaatctctt ggacgaccag
    92881 ttcatctgca atctgcaaat ccgccatctg ttgccataaa gaaagacgtt gatctattgg
    92941 tacattgaca gaatcaattc caatcaactg gatgtttcgc agaataaaag gcaagacagt
    93001 cgcgtttagt ttgattcccc cagcattgcc acataaagct actgcaccgt tataagagat
    93061 caatggaaga agactgctta gctgctcgcc acctactgtg tcgatgacat agtcgatttg
    93121 ctgttttcct aaagcttttg ttgtctctgg cagaaattca tcaggtcgta cgacttgcaa
    93181 agcacctaga ctttttagcc atgtgacaga ctcttctttt ctggaaaagg caatgattga
    93241 ctggaatcct agtttatgta acagggctat cgctgtactg cctacaccgc cagaagcacc
    93301 agtaacgaca acttttttat cggaggtcat accttgacgg attaaagcgt tcacacacaa
    93361 catagcagta aaccctgctg taccaaggat catcgcttga cgcagcgtca tattttttgg
    93421 tagaggaact aaccagtctc ctggtacttt ttgatattgg ctataaccgc ctggatgggt
    93481 gacgccaagc ccaaaaccag tagcaagtac aagatcttct tttgaaaaac gtggattgtt
    93541 actttggacg acagttccgg ataaatcaat ccctggagtc atcggatatg ttcgtatcac
    93601 ctgaccagac tcgctcattg ccaatgcatc tttataattg acatcagaat aggcaatttt
    93661 taccaagact tcattttcag ccaactcagt aaatggcaga tcactgattg ctgtatggaa
    93721 aacaggttcc tttattacct gaaaagatcg aaaagcgttc attcgtgaga cactcctttt
    93781 aaaattttct tttatcatac cataaagcct actcttttat tcttgttctt ccgtaaattt
    93841 agacaaaaat taggtgaaaa ataaagaatc aaacaactag aatttattcc aaaagataaa
    93901 gaaatattca gcaaaaaaaa ttttacaaag ggttgccttt ttcaataaat ttcgtatact
    93961 aatacgagtg gtagtaaaca ggaagtttac tattagaaaa cttcgattcg gaggaaatta
    94021 tggaaattgg tgagaagtta agaaatttgc gcatccaaaa gaatttgaca caagaagaac
    94081 taggcgaacg cacagatttg tcaaaagggt atatttccca gttggaacga gatttaagtt
    94141 caccatcaat ggaaacattc ttttctattc ttgaagtctt aggtgtgact ccagaagaat
    94201 tttttcatca agaagcagcg aatctgcaag tggtttattc aaaagaagat catacggttt
    94261 atttcgatga agaaaacggg tatgaattgg aatggctcgt ggctgattcg aatgaaaaag
    94321 aaatggagcc tgtcttgctg acttttgaag gtgcaggaga atataagacg tttgaacctt
    94381 cattatctga aacgtttatt tttgtattag aaggggaatt agagttaagc ttaggagaga
    94441 cggtctatgt ggcaaaagca ggacagtcga tctattatca agcgacaaaa caacatcaat
    94501 taaggaatca tctcaaaaaa cgaacaaaag tattgattgt tgcaacgcaa tcatatttgt
    94561 aatatagggg gtaaaaacgt ggggaaacca atcatttcat ttgatcaagt agtgaaacgc
    94621 tatgatgatg aaacagtatt aaaaaaagta agttttgaga tcgaacaagg aaaattttat
    94681 acactgcttg gtccttccgg ctgtggtaaa acaacgatcc tacgtattat agctggattc
    94741 accgaagcta cagaagggga catctatttt gaagggaaac ggttgaatga tttgccagct
    94801 aataagcgac aagtaaatac agtcttccaa gattatgcac tttttcctca tatgaatgtt
    94861 tttgataacg ttgcttttgg attgaagatc aagaaaatgt ccaaatcaga tatcgaaaag
    94921 aaggtaaaag atgctttgcg tatggtccag cttcctggat acgaaaaacg ggagattagt
    94981 gaaatgtctg gtggtcaaag acagcgtgta gcgattgcac gagcaattgt aaatgaaccg
    95041 aaagtattgc tattagatga accgttatcg gcgcttgatt tgaaactacg tacagatatg
    95101 cagtatgaac tgcgggatct gcaacaacga ttaggaatca cgtttatttt tgttactcat
    95161 gaccaagaag aagcgttggc aatgagtgat gagatttttg tcatgaataa agggaaaatc
    95221 gtccaaagtg gtacacccgt agatatttat gatgagccaa tcaatcattt cgttgctgat
    95281 tttgttgggg agagcaatat cgtggacggt gtaatgatcg aagataatct agttgagttt
    95341 gttggtaagc aatttgaatg tgtcgatggc gggatgcgtc taaatgaacc agttgaagtt
    95401 gttttacgcc cagaggattt gacgatcacg acaccagata aaggaaaact agtcgtaacg
    95461 gtcgatactc agctgtttcg tggtgtccat tatgaaatca tctgttatga tgaacagcaa
    95521 aatgaatgga tggtccattc aacgaaaaaa gctaaagaag gaagcaaagt aggattggcc
    95581 tttgagccag aagatatcca tgtcatgcgt ttcaacgagt ctgaagaaga attcgatgca
    95641 cgtcttgaca gctacgaaga atagggggga caagaatgaa aacaatgcgc aggatctatt
    95701 ccgtccctta tattttgtgg ctcggtttat tcgtgatcgc gcctgtatta atgatcattt
    95761 atcaatcttt ttttgatatg aatgggcaat tcacgctgaa taattatcta tcttatttta
    95821 cgtcggggac atcgctttct atgacgatca attctgtatg gtatgcattt ttgatcacgt
    95881 ttttcacgct gatcatcagt tacccgactg cttatttttt aacaaaactg aagcacaagc
    95941 aattatggct gatgctgatt attttgccta catgggtgaa tctgctgctt aaagcttacg
    96001 catttattgg gattttcagt gtgcacggca gcgtgaatca tttctttgat tttatcggga
    96061 tcgggccgaa acagatatta ttcacagact tcagttttct gttcgtggca acgtatatcg
    96121 agattccatt tatgattatg ccaattttca atgcattgga agagatgaat ccttctttga
    96181 tcagtgctag tagagattta ggcgcgaata gctttgagac cttccgacga gtgatttttc
    96241 cgttatcatt aaatggtgtg aaaagtggtg tccaagcagt atttattcct tctctttctt
    96301 tattcatgct gactcgtctg attggcggaa atcgggtgat cactttagga acggctatcg
    96361 aacagcattt cttggttaca caaaactggg ggatgggttc tacaatcggt gtgattttaa
    96421 tcattgctat gttcatcgtg atgctcctga caggcgagaa gaaaaaagga ggtcgctcat
    96481 aaatgaaaaa atttaaatgg tcgtatcttt atttagggat cgtcttttta ttcttatatg
    96541 caccgatctt ctatctgatt ttttattcct tcaatgaagg aggaacgatg aacagcttta
    96601 caggatttac ttgggaaaat tatgaagctg tttttgaaga tacacgtttg atcattatcg
    96661 tattgaatac gttcatgctg gctttcttat ctgcattgat cgccacgatc atcgggacat
    96721 ttggggctat gggaatctat tacacgaaaa aacgacgtgc acggacagcg ttgttgagca
    96781 tgaataacat cttattagtc tctcctgatg ttattatcgg tgctagtttc ttgattttct
    96841 ttacactact aggattcgga ttaggttttc agagtgtatt gctttcccat attgctttca
    96901 gcattccaat cgtcgtcttg atggtcttgc caaaattgca ggaaatgaac gattcaatgg
    96961 tcgatgcggc tagagacctt ggtgctaata atttccaagt agtcaaaaat atcattttgc
    97021 cttatctttc tccaggaatc atagctggat attttatggc atttacctat tctcttgatg
    97081 attttgcggt tacatttttc gtcacaggaa atggctttac gacgctctca gtagagatct
    97141 attctcgtgc aagacaagga atcagtttag aaatcaatgc gctaagtgcg ttagttttct
    97201 tgttctccat gatattggtc attggatact actttattag taaagaaaac cgtccaaaac
    97261 ggctgaagaa aaacagaaga gtacgcgagg gggtggcacg gctgcgatga aaaaattaca
    97321 atctctgttt ttaggtattc tcatcattat tgcggtgctg ctgcttggtg ttcgtcaact
    97381 tgaaaaaaca agtggcatga gtggagcaaa ggtacttacc atctataact ggggagatta
    97441 tatcgatcct agtttgatca cgaaatttga aaaagaatat ggatacaaag tgaattatga
    97501 aacatttgat tctaatgaag cgatgttcac aaaaatccag caaggtggga caaactacga
    97561 catcgctata ccaagtgaat atatgatcca aaaaatgatc aaagaaaaac tagtacttcc
    97621 attagatcat tcgaagatca aaggattaaa taatattgat ccacgatttt tagacttgga
    97681 ttttgatcca gataatcagt attcgattcc atatttttgg ggaactttag ggatcgtata
    97741 taacgataaa ttctttgatg caaatcaaat taagcattgg gatgatttgt ggaagccgga
    97801 attaaaagac agtttgatgc tgattgacgg tgcacgagaa gttatggggc tttctttgaa
    97861 tagtttaggc tattctttga atagtaagaa tatggcacag ttgacagaag cagcaaataa
    97921 attgaatcat ttaacaccaa atgtcaaagc aatcgtggca gatgagatca aaatgtatat
    97981 gatcaatgaa gaagcctccg tggcagtgac attttctggc gaagctgccg atatgatgtg
    98041 ggaaaatgaa catttacatt atgtgatccc accagaagga tccaatcttt ggtttgataa
    98101 tatcgttatg ccaaaaaccg tgaaaaataa agaaggtgcc tatgatttca tcaactttat
    98161 gctagaaccg gaaaatgcgg cacaaaatgc agagtatatt ggttattcta caccaaatga
    98221 agaagcaaaa gcgttattgc caaaagacat ttcgtcggac gaacaattct atccatctga
    98281 tgacacgatc tcccatcttg aagtctacaa agatctagga tcgaaatacc taggcatcta
    98341 taatgaccta ttcttagaat tcaagatgta taggagataa tggcaaaact cagtaagaat
    98401 ggggacttat caacgttcta aagagctaaa aatcgtcctt tttctatctg agttttccaa
    98461 tcttctacca aaagcggatg aaacgccgct ttttttgtgt tattttattt tttctaccac
    98521 gtttttctac cagagaaaag tggacaaaac cattgtaaaa tagggcccga gacgggctag
    98581 taaatctaag attgaaaaag ttacagaagg ataaagtaat tggaaactat gcaaatatac
    98641 ttaacccttc tgtaatctat taataaaact aatatatgaa tccagttcaa tatttgtagc
    98701 tatgaaaaaa cataaaaatc atattagata aaaactaaat tggtagataa attattttgt
    98761 cagatatctg aggcaaatat tcgactattt gtgtttcaaa gtaaaaattg gaatacaaat
    98821 atagaataga catattgcag ccgatagttt attatgcttt tagacacatt aaactggagg
    98881 aaaaaatgaa aaagagcgct aaagatcgag tttttgatga gctgatacga ctaacaggga
    98941 atatgtcttc tgtcactgcc gagaaaattt cgaagaatct aggtatctct cgtcagaatg
    99001 ccagccatta tttgacgaga ttagtggaag ataaaaaggt cgagaaatta ccgggaaagc
    99061 ctgttttgtg gaagccttta gatgaatatg cggttgtaga taatacggag caaataaacg
    99121 aagcctttca ttctgtagtt ggtcatgacg gaagtctgag agaggtcatt caaaaatgta
    99181 tagccgctgt caaatatccg cctaacggac tcagtgtatt aataaatgga gccacgggtg
    99241 ttggaaaaag ttttttagcc acaaaaattt ttgaatacgc tatccatgag caaattattg
    99301 aaaaagatgc accatttgct atcttaaact gtgccgatta tgcggacaat cccgaactgc
    99361 tttcggcaac actgtttgga tacaagaagg gggcctttac gggggctgaa aaggataccg
    99421 atggtttatt ggcaacggcc aacaacggct atctttttct cgatgaggtc catcgcctat
    99481 cgaaagaaaa tcaagaaaag ctctttctat ttattgatac tggaaactat cgtccagtcg
    99541 gtgaaaatgt aaattggcat tctgctaagg ttcgttttat tttcgccacg acggaaaaag
    99601 gtgaaaatta tttattagac acctttgatc ggcgtattca aattagtgtt atgttgccaa
    99661 cttttgaaga tcgccccatt cgagaacgat tagagctgat tcaattattt tttcaaaatg
    99721 aagcggatgt tttacaaaaa gatattattg tttcaagaga agccttgtct ttaatgttat
    99781 ctcatccctt tagtgggaat attggtaagc tgaagaatat cattaaaatc agttgtgcgg
    99841 atgtgtatag tcgaacacaa gaagaaccat taacgattgg gaaaaatgag atcttgattc
    99901 agttaaatat gctggaatcc gaagtagaaa gcttgccgat tgctagttta ttgataaaga
    99961 aaaatcaaaa gataaaaacg gatcgaatca aaccgtttga ttatgattcg attttggaaa
   100021 aaattactca gctgataaat gaaggtaatt tcaatgaaaa tttttcttcg ataaagaatg
   100081 aagtacagca tttggtgcat cacattgata aggattcttt tttatcaatg agtagaaaat
   100141 tgatgattca gatgtttaga aaggtctggg tatccgtttt aggaaaaacg tacggattga
   100201 cgacgagtgg acctgttgca gaaattatta gcagatccta tgtcatttcc ccaagctata
   100261 agacagactt aaccgggata aatcgtacat tagctgcaaa tctacctcgg acgacttact
   100321 tatgtaatca gttcttggag catttaccac cgctctcaga aaaggacgag aactttctaa
   100381 gactgcttat tgtcgtgact ttatctgatt atgtagatga atccatcgag ctgaagggcc
   100441 tgttgcttgc tcacggagat agtacggcta gcagcattca agcagtagtg aatcaattat
   100501 gcggcaatta tgtttttgaa gcgttggata tgccagtaga gagtaatgtg tcagagattg
   100561 tcgaatcgac aaggaatttc gtgaaacggc aagtgaatac agatagtcta gtgctgatcg
   100621 tagatatggg ctctttaaat caaatttaca cacagatcaa gaacgattta aaaggggagt
   100681 tacttcttct gaataatttg acgacttcca ttgcacttga tattggcttg aagatgacga
   100741 tgaatgcgcc atttaaacag attgcggagg atgcagcaga caagtattca atcaatgttc
   100801 aatactttga aggcttttct caaagtagca acattatcat ttcctgtatg tcgggtttgg
   100861 ggatttcgga caaattgaaa gatgtttttc gtcatcattt atcggatgaa tccatcgaag
   100921 ttttcacgaa ggactacaga gaactacgtg aattaatcga ccaaaatgat gaaacttatt
   100981 ttgacaagac aaaattggtc atcacgactt cggatcttcc acgttccttt tcaattccta
   101041 atattaatgt ttatgacatc ttagacggtg atggagcagc caatttggag catattttga
   101101 gtcctgaaat gaagccggca gaatttaacc gcttgattca ggagctagta aaattctttt
   101161 cgttggaagg aattgcggac cgtttgaact ttctgaaccc aacgatcgtg atgagtgaag
   101221 tggaaacagt catttctaaa tatgaaaatt actatcattt cactttaaat ggccgtgtaa
   101281 aactaaatct ctatatgcat acggcattga tgatggaacg tttattcttg gctcggacag
   101341 aaaaatctga agaaccgaat tatgatttga aaccagatga agatgaattt tattatgttt
   101401 ctagaagtat ttttcaatca atggagatga aatataattt taaagtcaat gcttatgagc
   101461 tttcgctgct ctatgaatta ttaggaccgt atattcaaaa ataaagtgtc taaaaaagtg
   101521 tctaatctgt tttttttagg ttagacactt tttttaatgt gaattaacgt gattaaacca
   101581 atgtttttaa aagttggcac aggaattgct tatataaaag tgaacagaaa gaaaggaatt
   101641 aaccatatga agaaaattct aatagcgact cacggtcact tagcttcagg ctttcttagc
   101701 agtattcaat tactgacggg gaaggttcaa gagattacgg tgattaatgc ttatgtggat
   101761 gagtgtgatt ttgaacagga gttgaatgta tttgtaaacc aggtgaacga gcaggatcag
   101821 atttttgttt ttaccgattt atttggcgga agtgtcaatc agaagattac caaaaccttt
   101881 ttggagaaag agattgctgt aactattttg tctggcttta atctgccaat tctgctcgaa
   101941 atagtattgg caaccaagga attaacaaag gaggatattc aggaactggt aaataaatgc
   102001 caacaggaat taaagatgac cattcttgat gaggtacatg tagtaaatga agaggaagat
   102061 tttttttagg aggaagaaaa atgattaaag cagtgcgaat tgatgaacga ttaattcatg
   102121 gacaagtagc gatgacttgg acaaagagtc taaatttaac cgggttagta gtagcaagcg
   102181 atgaggcggc taccaacgat attcaaaaga tgacattgaa gatggctgca ccatcgaatg
   102241 tcaaagtcat tattaaaaca gttgatgacg cgattcattt gttaaaagat ccgcgggcga
   102301 aggacatgcg cttgatggtt ttagtcccta atgtcaaaga tgcggtaaaa gttgcgaaag
   102361 ctttcccaac ggaaatcgaa ttaatgaacg tggggaatgc tgggaaaatg acaccgccat
   102421 cggaagataa gaaaacctta tcaaaggaag tgatgttaac gacgtcagaa attcaagact
   102481 taaaagaatt agttgaactt tatccagata ccgtttttca gcctacaccg gcgatggaaa
   102541 aacgttcagc tatctctatt ttgagtgatt tttagaaaaa ggaaggagaa aaattatgta
   102601 tgatgcattt gtagtcgctt tggccgtttt tattggaatt gctggacatg aaatgttcgg
   102661 gatgtccatg ctgagtcgtc cgattgttgt tgctccgtta gcaggagcgt tgatggggga
   102721 tttgcagacg gggttgattg tcggggcgtc actagaagcc atctttatgg gtgtcgtcaa
   102781 tatcggaatc tctagtacgg cggagccttc tttggctgct gggctggcta cagcgttcgc
   102841 aatcaaaatg ggaggaaacg tggatgcgat tattcccatt gtctttccac tagcagtatt
   102901 aggattacag ttaatgaata tgattttttc ttttgtatgt ggaccgatgg ctgctaaatt
   102961 tttcaaattt gcaaaatctg gtgatcaaaa aggaatcatc aatctgcact tctttatgtg
   103021 gtttgtccac tatgcgcttt atgctcttat tccattcttt gccgttttat ttggagcaga
   103081 tgttgtcaaa agcgtcttgg ataatattcc agaggtcatc atgaacggat taacagtcgc
   103141 tggaaatctg ctgccggcag ttgggatggc gatgctgtta cggatgcttt ggagtaaaaa
   103201 tattgctgtg tggtttttct ttggggccgt tgttatggcg tatctcgagt taccattgat
   103261 tgcaattgcc gcagttggaa cgattattgc aattgttata gcccaaagag atctacaact
   103321 aaaaaatata tctgttcatg cagttcaaag cgatgctgct gtaaatgcaa cgcttgacca
   103381 agaagaggag gatttctttc aatgaaatta gctgatggaa tttctaaaga tgagaaaaaa
   103441 ttggttcgga agatgttttg gcgctcgatg actatgtatg cttcggtgaa tccggtgacg
   103501 atgggcggcg gtggtttcgc ctattctatg atgcccttca ttaatcattt ctacaaaagt
   103561 gaagaagatc gagctgcggc gctggaacgt catacacagt atttttcgac gaccattcct
   103621 tgcgcgtcct ttgtcatggg gattgcggca tcgatggaga aggaaaattc tgaaaaagct
   103681 ggttttgacg tggaaagtat caacgcgatc aaaacgagtt tgatgggacc attggctgga
   103741 attggcgatt cattgttctg gggagtttgg cgtgtcgtta ccgcgggact agcgatcaac
   103801 ttagccaatg ccggaaatat tttggcacca attatctttt tagttatgtt caatatacct
   103861 aattacttga tgcgatacta tggcggcgtt ttaggctatt cattaggctc taaatatatt
   103921 gaaaagctgt atgcgaacgg gttaattgaa attctgacaa aagctgctag tatcttgggt
   103981 ctgatcatgg taggtgcaat gacgatcagt atggtcaaat tcaataccgt tttgagcttt
   104041 tcaatgaagg gcgaagaact aatgaatgtc caagaaatcc tcgatagtat ttttaaagga
   104101 attgtaccgt tattggtaac attgagctgt ttcaaattac tagataaaaa agtcggcgtc
   104161 atcaaaatta tttttggaat ggtcgtcttg ggaattttat tgtcattact tggaatcgca
   104221 tagtaggaag ctgcagctac taggctgcag tttttttagc aaggagggaa aagatgacta
   104281 ttaatgaagt aatttattca cccaaacacc cattttttaa tgctgagtta aacgacggaa
   104341 atgtgactac ttcaaacgaa tacagctact atcggaatat tcctgatgta aagggattga
   104401 ccttcgatgt aagtaatcaa aagacattag caaaccttga tgtttgcggg acgattcaaa
   104461 cgttgacctt ttatcgagaa aatttactaa ctgaggaaaa accaggtgtt tgggtcaata
   104521 aacagctctc tcaaaccaat tcgttatcct taaaggttga agtaaacggg attgtctacc
   104581 atttgtcaga atcggatcat cgtattgaag tcgatgtgat tcaagactgt ttgcctagat
   104641 atattcatca ttattcgacg tttaaggtca ttgtagtatc ctttgctccc atactggcta
   104701 ataagcgttt aagtatgctt gttcaacagg tttacattgt gaatgaaaca cacgaaagcc
   104761 ttcaagtttc agtgaaagat gtgccttcgt atcaagagaa gtattcggat cagcaaaatg
   104821 tgttgatttc tcaaaaagga aataaggaca cgatcgcaca acaggaagtg gcaagcttcg
   104881 ccattgcttt tgtggaccca aatgcttttg aagaagtaat gacttttcag gaatctgata
   104941 tcgaaaaatg gctgaaggaa acattgaact attttgctca gctatttggg cagctgtcaa
   105001 tgccaaacga tagagttgtc catctctatc gtcgtgcact ttatcaaagc ttttctagtt
   105061 tcggtatgaa tcgacaggga caggtcgtcg gcagcaattg gggcagctat cctgctacta
   105121 atcgaatttg gaataaggat atgtattacg catcattgcc attcaccatg tttgaaccgg
   105181 agttatgtca aaaaacggtt ctatggtttg atcaatatgg cgttaagttt cctggttcaa
   105241 aatttacagg aggtatcaat cattctctga gtaattcgtt atccagtgcg ttactggcta
   105301 gtctttattt tgaacatact ggggatacaa aattctttga gcggtgtccg caggttctac
   105361 tgaatgcttc aagaattatt gaggacatat tagctcaacg tcggtcaggg gaaccaatgc
   105421 tttttccatc tgtttggtta tcagacgcct ttgcacttgg aaagtatcat accggcagca
   105481 atctttgtct ttggaaggcc tgtattggac ttgcagaaat ttttgaagcc ctagaacaac
   105541 tgagtcttag caagcgatac aaaaacattg cctgtaaact aaaggaatca atttgtaaac
   105601 aaatgactat cgatggtcct tttggtgagc aatttttgga gggaatcggt gatgaggaga
   105661 aaacaaccta ttcagtgaag cactatcaaa aaccgattct tgagcaaggg ttaatctttt
   105721 taagtgatgt gattgtggat ggaaaaatta atttattgat gcatgatgga gaagaatcag
   105781 acactacgtt gatgcctttt tatcaatttt tagataataa agatcattta tatcagaaca
   105841 ccatggcctt ttctgctagt tctttcaatc caacctatag cgaggaaatc aaaggcatca
   105901 cttggggatt agaatccggc gccacttttc ctggctttat cactgtttta atgagcgagc
   105961 tgcacaaccc tcaagctttc gtaacccgat tagaagaact aatttgtttg gcagatttag
   106021 acggttcgtg gtggtggtgg ccgtatcgct tagaggctca acaaggagaa gtcgtgcgag
   106081 actttggctg cggcaaatgc ggctgggcat cgggtctttt tgtcagccta atgatcagtc
   106141 aatatttcgg attgaaattc aaaaaaggag tattgcaaat tgatcctgtt gataatttaa
   106201 cgttttcttg gaagaatttg aagttcggtc aagccttcat ttcgattgaa tgtacgcaat
   106261 ccgagttgag tatttcaaat ttgtcagaaa agccattgaa aataagtgat acgaaaaaaa
   106321 ttcttcacgt agaaaaggga aaaacgattc acattcaaag gagaaaaaga tgagtatact
   106381 aaaaaatatt acccaattta aaaaaatcca gagtcgagca atcactcccg aaaatccaac
   106441 aggcgagaaa ggtaaagctg cgatgttgcc aagcgagcta ggaccaagcc gcaaaggtcg
   106501 tggcgcaatt ccgctgactt ctggtgaaga aactgtgatt gcggatatta ctggaacggg
   106561 aataattcgt catatgtgga tgacgattcg tgatcgaaca gaatttggca gttttgtttt
   106621 acgtgattta atcttaagaa tttattggga tgggtcggat agccctgctg ttgaaagtcc
   106681 tttaggggat ttcttttgta atgggtttgg agagcgttgc gaggtgaact cgttgccgat
   106741 agtggttaat cctacagggg gctttaattc ctatttcgaa atgcctttcc aaacatccgc
   106801 taaaattacg atcgagaatt tacatccgaa ggatatgtcc tcattttttt atacgataaa
   106861 ttacgctttg gtggacgaat tggaagaaaa tagcctatat tttcacgcaa attggcatcg
   106921 ggaacgcatt accaaattac aggaggatta taagttaatt gatcgattaa agggtcaagg
   106981 ctattacgtg ggtacttacc ttgcgcttac ctgcctagaa cgttattggt ggggcgaagg
   107041 cgagtttaaa ttctacattg atggggatag tgagtatccg accgtaacct caacaggttc
   107101 agaagattat tttggcgggg cgtgggcctt tcatgagtac gatgagttcg gacgtccaca
   107161 tgcgaaaaac tattcaacag cctttttagg gtatccttac caatcaaata gagatgccac
   107221 aagagatcgt tttgaaacgg gaaaattgaa tgccgttcat gcctttggtg acgacgcaat
   107281 gccacgacat ggtctttatc gttggcatct tcaagacccc atctgtttta ccgaagattt
   107341 atcggtcact ttacaacaaa tcggcaacga tgatgttcgc ttatttgagc ggcaagacga
   107401 tgttgctagt gtcgcttact ggtattcaac agaagcttca ggaaatacta aacgtcattt
   107461 cactgatcga gaacgcttgc cgcgttaatg aagtgttttt cacatattcg aagggaggaa
   107521 cctatgatga tatatggggc aattgaagct ggagggacta aatttgtctg tgcagtcggt
   107581 actgaggaat taacgatttt ggaaagagtc agttttccca caacaactcc agttgaaaca
   107641 atgaagcagg tcattgattt ttttaaacct tttaaggaac aattggcagg catcggggtc
   107701 ggctgctttg gtccaattga tattcaatgc aaatcaacga gtttcggtca tatcacatca
   107761 acaccaaaac ttgcttggca aaatttcaat tttgtaggaa cgctaaagca atatttcaat
   107821 attccgattg aatggacaac agatgtcaat gcggcttgct atggtgaata tgttgctggt
   107881 tctggcaaag ggttagcagg tgttgcatac ttcactgtgg gaacaggtat cggaggtggt
   107941 gccttgttca atgggacttt cgtggaaggg ttcagtcatc cggaaatggg gcatatgttg
   108001 atcaagcgac atccaaagga caatttttca ggaaactgtc ccttccacca agattgttta
   108061 gaggggatgg cagcaggacc agcgattgaa aaacggttgg gtgtaaaagg ccagaattta
   108121 ttggctgacg attcgttttg gcagattgaa gccttttacc ttgcccaatg tgcatacaat
   108181 acaacactga tgttttcgcc agatagaatt atttttggcg gtggtgtaat gaaacaagaa
   108241 cacatgaaga aaaaagttca ggataaattt gttgagctta taaatggata tgttgaaata
   108301 ccaccaattg atagctacat tattacgccc gaattaggtg ataatgccgg aataatcggc
   108361 ggattagcat tggccagaaa agcggtaaga aataagcaac cataattaat gaatgtttca
   108421 ttgatactca tgtcaaaagt atttaaccga gtaatcaaat gatcaaatga agttggataa
   108481 agcgaaaatg aattcaaata aaaaggctct aagtcaaatc gtgttgtttt cattaaatca
   108541 ctgaaaggaa atgcttctgc gtttcctttt ttctttttgt tgtgcgctgt ataccttgag
   108601 gatattcact ggtggcgtac acttaaatag taatatattg tgttacttta tgtctgtggt
   108661 aatagttata gttgcttacc aatgtatgtt catttttcga aatgttgact tcataatcct
   108721 tggcttaatg tttgaatttt tttggtgata gattttttca gccgtataaa ataaatcgta
   108781 attctattat agagagttgt gattataaaa agatagcaag aaaaaaaaag aaagtttcta
   108841 tacattttaa atattttatt cctatttcta ttgctaaaaa agtagaaatt ctatatttac
   108901 ctttaactct cgcttctctt gcaaatgtag tta
//
LOCUS       DAYYFU010000003       105427 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000003 DAYYFU010000000
VERSION     DAYYFU010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 105427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 105427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..105427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.003"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACHA34_000211"
     CDS             212..604
                     /locus_tag="ACHA34_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8790894.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACHA34_000212"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACHA34_000212"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFM8790895.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACHA34_000213"
     CDS             2698..2943
                     /locus_tag="ACHA34_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8790896.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACHA34_000214"
     CDS             3165..4775
                     /locus_tag="ACHA34_000214"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFM8790897.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACHA34_000215"
     CDS             complement(4752..4964)
                     /locus_tag="ACHA34_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8790898.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACHA34_000216"
     CDS             5067..5936
                     /locus_tag="ACHA34_000216"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFM8790899.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACHA34_000217"
     CDS             6101..7372
                     /locus_tag="ACHA34_000217"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8790900.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACHA34_000218"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACHA34_000218"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFM8790901.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACHA34_000219"
     CDS             8763..9026
                     /locus_tag="ACHA34_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFM8790902.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACHA34_000220"
     CDS             9471..9920
                     /locus_tag="ACHA34_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8790903.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACHA34_000221"
     CDS             9977..11395
                     /locus_tag="ACHA34_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8790904.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACHA34_000222"
     CDS             11395..12255
                     /locus_tag="ACHA34_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8790905.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACHA34_000223"
     CDS             12429..13202
                     /locus_tag="ACHA34_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8790906.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACHA34_000224"
     CDS             13349..13714
                     /locus_tag="ACHA34_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFM8790907.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACHA34_000225"
     CDS             complement(13862..15070)
                     /locus_tag="ACHA34_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8790908.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACHA34_000226"
     CDS             complement(15089..15367)
                     /locus_tag="ACHA34_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8790909.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACHA34_000227"
     CDS             15560..16216
                     /locus_tag="ACHA34_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8790910.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACHA34_000228"
     CDS             complement(16658..17455)
                     /locus_tag="ACHA34_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFM8790911.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACHA34_000229"
     CDS             complement(17452..18636)
                     /locus_tag="ACHA34_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFM8790912.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACHA34_000230"
     CDS             complement(18661..19908)
                     /locus_tag="ACHA34_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8790913.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHA34_000231"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHA34_000231"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFM8790914.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACHA34_000232"
     CDS             21888..22202
                     /locus_tag="ACHA34_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFM8790915.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACHA34_000233"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACHA34_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFM8790916.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACHA34_000234"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACHA34_000234"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFM8790917.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACHA34_000235"
     CDS             23546..23875
                     /locus_tag="ACHA34_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFM8790918.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACHA34_000236"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACHA34_000236"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFM8790919.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACHA34_000237"
     CDS             24863..25687
                     /locus_tag="ACHA34_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFM8790920.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACHA34_000238"
     CDS             25680..26027
                     /locus_tag="ACHA34_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFM8790921.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHA34_000239"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHA34_000239"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFM8790922.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACHA34_000240"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACHA34_000240"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFM8790923.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHA34_000241"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHA34_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFM8790924.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHA34_000242"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHA34_000242"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFM8790925.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACHA34_000243"
     CDS             31684..32451
                     /locus_tag="ACHA34_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8790926.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACHA34_000244"
     CDS             32441..34483
                     /locus_tag="ACHA34_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8790927.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="ACHA34_000245"
     CDS             34764..34913
                     /locus_tag="ACHA34_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFM8790928.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="ACHA34_000246"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="ACHA34_000246"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFM8790929.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="ACHA34_000247"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="ACHA34_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFM8790930.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="ACHA34_000248"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="ACHA34_000248"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFM8790931.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="ACHA34_000249"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="ACHA34_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFM8790932.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="ACHA34_000250"
     CDS             39330..40412
                     /locus_tag="ACHA34_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFM8790933.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="ACHA34_000251"
     CDS             40412..41215
                     /locus_tag="ACHA34_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8790934.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="ACHA34_000252"
     CDS             41285..42025
                     /locus_tag="ACHA34_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8790935.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="ACHA34_000253"
     CDS             42036..43040
                     /locus_tag="ACHA34_000253"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFM8790936.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="ACHA34_000254"
     CDS             complement(43085..44929)
                     /locus_tag="ACHA34_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8790937.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            45141..45659
                     /locus_tag="ACHA34_000255"
     CDS             45141..45659
                     /locus_tag="ACHA34_000255"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8790938.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            45787..46470
                     /locus_tag="ACHA34_000256"
     CDS             45787..46470
                     /locus_tag="ACHA34_000256"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFM8790939.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            46779..46852
                     /locus_tag="ACHA34_000257"
     tRNA            46779..46852
                     /locus_tag="ACHA34_000257"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46813..46815,aa:Arg,seq:tct)
     gene            47011..47370
                     /locus_tag="ACHA34_000258"
     CDS             47011..47370
                     /locus_tag="ACHA34_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFM8790940.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            47493..49124
                     /locus_tag="ACHA34_000259"
     CDS             47493..49124
                     /locus_tag="ACHA34_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFM8790941.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(49186..49827)
                     /locus_tag="ACHA34_000260"
     CDS             complement(49186..49827)
                     /locus_tag="ACHA34_000260"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8790942.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    50013..50089
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            50109..50417
                     /gene="rplU"
                     /locus_tag="ACHA34_000261"
     CDS             50109..50417
                     /gene="rplU"
                     /locus_tag="ACHA34_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFM8790943.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            50444..50788
                     /locus_tag="ACHA34_000262"
     CDS             50444..50788
                     /locus_tag="ACHA34_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFM8790944.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            50801..51094
                     /gene="rpmA"
                     /locus_tag="ACHA34_000263"
     CDS             50801..51094
                     /gene="rpmA"
                     /locus_tag="ACHA34_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFM8790945.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            51528..54335
                     /locus_tag="ACHA34_000264"
     CDS             51528..54335
                     /locus_tag="ACHA34_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8790946.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            54526..55017
                     /locus_tag="ACHA34_000265"
     CDS             54526..55017
                     /locus_tag="ACHA34_000265"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8790947.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            55120..56109
                     /locus_tag="ACHA34_000266"
     CDS             55120..56109
                     /locus_tag="ACHA34_000266"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8790948.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            56142..56945
                     /locus_tag="ACHA34_000267"
     CDS             56142..56945
                     /locus_tag="ACHA34_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFM8790949.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            56966..57880
                     /locus_tag="ACHA34_000268"
     CDS             56966..57880
                     /locus_tag="ACHA34_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8790950.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            57963..58337
                     /locus_tag="ACHA34_000269"
     CDS             57963..58337
                     /locus_tag="ACHA34_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFM8790951.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            58476..58721
                     /locus_tag="ACHA34_000270"
     CDS             58476..58721
                     /locus_tag="ACHA34_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8790952.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            58893..59957
                     /locus_tag="ACHA34_000271"
     CDS             58893..59957
                     /locus_tag="ACHA34_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFM8790953.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            60203..60628
                     /locus_tag="ACHA34_000272"
     CDS             60203..60628
                     /locus_tag="ACHA34_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8790954.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            60621..61070
                     /gene="nusB"
                     /locus_tag="ACHA34_000273"
     CDS             60621..61070
                     /gene="nusB"
                     /locus_tag="ACHA34_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFM8790955.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            61167..62021
                     /locus_tag="ACHA34_000274"
     CDS             61167..62021
                     /locus_tag="ACHA34_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFM8790956.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            62022..63359
                     /gene="xseA"
                     /locus_tag="ACHA34_000275"
     CDS             62022..63359
                     /gene="xseA"
                     /locus_tag="ACHA34_000275"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFM8790957.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            63364..63588
                     /locus_tag="ACHA34_000276"
     CDS             63364..63588
                     /locus_tag="ACHA34_000276"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFM8790958.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            63590..64480
                     /locus_tag="ACHA34_000277"
     CDS             63590..64480
                     /locus_tag="ACHA34_000277"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8790959.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            64489..65307
                     /locus_tag="ACHA34_000278"
     CDS             64489..65307
                     /locus_tag="ACHA34_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFM8790960.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            65414..65863
                     /locus_tag="ACHA34_000279"
     CDS             65414..65863
                     /locus_tag="ACHA34_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8790961.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            65881..67563
                     /gene="recN"
                     /locus_tag="ACHA34_000280"
     CDS             65881..67563
                     /gene="recN"
                     /locus_tag="ACHA34_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFM8790962.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(67618..67734)
                     /locus_tag="ACHA34_000281"
     CDS             complement(67618..67734)
                     /locus_tag="ACHA34_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFM8790963.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(67807..68763)
                     /locus_tag="ACHA34_000282"
     CDS             complement(67807..68763)
                     /locus_tag="ACHA34_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8790964.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            68934..69302
                     /locus_tag="ACHA34_000283"
     CDS             68934..69302
                     /locus_tag="ACHA34_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFM8790965.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            69472..69903
                     /gene="mraZ"
                     /locus_tag="ACHA34_000284"
     CDS             69472..69903
                     /gene="mraZ"
                     /locus_tag="ACHA34_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFM8790966.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            69924..70883
                     /gene="rsmH"
                     /locus_tag="ACHA34_000285"
     CDS             69924..70883
                     /gene="rsmH"
                     /locus_tag="ACHA34_000285"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFM8790967.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            70888..71289
                     /gene="ftsL"
                     /locus_tag="ACHA34_000286"
     CDS             70888..71289
                     /gene="ftsL"
                     /locus_tag="ACHA34_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFM8790968.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            71290..73482
                     /locus_tag="ACHA34_000287"
     CDS             71290..73482
                     /locus_tag="ACHA34_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8790969.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            73508..74470
                     /gene="mraY"
                     /locus_tag="ACHA34_000288"
     CDS             73508..74470
                     /gene="mraY"
                     /locus_tag="ACHA34_000288"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFM8790970.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            74484..75845
                     /gene="murD"
                     /locus_tag="ACHA34_000289"
     CDS             74484..75845
                     /gene="murD"
                     /locus_tag="ACHA34_000289"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFM8790971.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            75871..76959
                     /gene="murG"
                     /locus_tag="ACHA34_000290"
     CDS             75871..76959
                     /gene="murG"
                     /locus_tag="ACHA34_000290"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8790972.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            76974..78194
                     /locus_tag="ACHA34_000291"
     CDS             76974..78194
                     /locus_tag="ACHA34_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFM8790973.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            78350..79675
                     /gene="ftsA"
                     /locus_tag="ACHA34_000292"
     CDS             78350..79675
                     /gene="ftsA"
                     /locus_tag="ACHA34_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFM8790974.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            79698..80939
                     /gene="ftsZ"
                     /locus_tag="ACHA34_000293"
     CDS             79698..80939
                     /gene="ftsZ"
                     /locus_tag="ACHA34_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFM8790975.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            80956..81633
                     /locus_tag="ACHA34_000294"
     CDS             80956..81633
                     /locus_tag="ACHA34_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFM8790976.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            81647..82246
                     /locus_tag="ACHA34_000295"
     CDS             81647..82246
                     /locus_tag="ACHA34_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFM8790977.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            82327..82548
                     /locus_tag="ACHA34_000296"
     CDS             82327..82548
                     /locus_tag="ACHA34_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFM8790978.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            82597..83379
                     /locus_tag="ACHA34_000297"
     CDS             82597..83379
                     /locus_tag="ACHA34_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFM8790979.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            83400..84107
                     /locus_tag="ACHA34_000298"
     CDS             83400..84107
                     /locus_tag="ACHA34_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFM8790980.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            84369..87167
                     /gene="ileS"
                     /locus_tag="ACHA34_000299"
     CDS             84369..87167
                     /gene="ileS"
                     /locus_tag="ACHA34_000299"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFM8790981.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(87216..88742)
                     /gene="zwf"
                     /locus_tag="ACHA34_000300"
     CDS             complement(87216..88742)
                     /gene="zwf"
                     /locus_tag="ACHA34_000300"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFM8790982.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(88757..89404)
                     /locus_tag="ACHA34_000301"
     CDS             complement(88757..89404)
                     /locus_tag="ACHA34_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8790983.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(89588..89917)
                     /locus_tag="ACHA34_000302"
     CDS             complement(89588..89917)
                     /locus_tag="ACHA34_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFM8790984.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            90094..90822
                     /gene="sfsA"
                     /locus_tag="ACHA34_000303"
     CDS             90094..90822
                     /gene="sfsA"
                     /locus_tag="ACHA34_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFM8790985.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            90838..91851
                     /locus_tag="ACHA34_000304"
     CDS             90838..91851
                     /locus_tag="ACHA34_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8790986.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            91851..93128
                     /locus_tag="ACHA34_000305"
     CDS             91851..93128
                     /locus_tag="ACHA34_000305"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8790987.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            93191..95893
                     /locus_tag="ACHA34_000306"
     CDS             93191..95893
                     /locus_tag="ACHA34_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8790988.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            96045..96362
                     /locus_tag="ACHA34_000307"
     CDS             96045..96362
                     /locus_tag="ACHA34_000307"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8790989.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            96392..96712
                     /locus_tag="ACHA34_000308"
     CDS             96392..96712
                     /locus_tag="ACHA34_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8790990.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            96820..98280
                     /locus_tag="ACHA34_000309"
     CDS             96820..98280
                     /locus_tag="ACHA34_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8790991.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            98348..98569
                     /locus_tag="ACHA34_000310"
     CDS             98348..98569
                     /locus_tag="ACHA34_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8790992.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            98600..98782
                     /locus_tag="ACHA34_000311"
     CDS             98600..98782
                     /locus_tag="ACHA34_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFM8790993.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            98782..99195
                     /locus_tag="ACHA34_000312"
     CDS             98782..99195
                     /locus_tag="ACHA34_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFM8790994.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            99318..100499
                     /locus_tag="ACHA34_000313"
     CDS             99318..100499
                     /locus_tag="ACHA34_000313"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8790995.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(100570..100734)
                     /locus_tag="ACHA34_000314"
     CDS             complement(100570..100734)
                     /locus_tag="ACHA34_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8790996.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            100888..100961
                     /locus_tag="ACHA34_000315"
     tRNA            100888..100961
                     /locus_tag="ACHA34_000315"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100922..100924,aa:Arg,seq:cct)
     gene            complement(101030..102169)
                     /locus_tag="ACHA34_000316"
     CDS             complement(101030..102169)
                     /locus_tag="ACHA34_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8790997.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEVK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINAVFDSLYKNDN"
     gene            complement(102279..102929)
                     /locus_tag="ACHA34_000317"
     CDS             complement(102279..102929)
                     /locus_tag="ACHA34_000317"
                     /inference="COORDINATES: protein motif:HMM:NF023043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFM8790998.1"
                     /translation="MKKNLILGFICTSILTLGACSSPSNNATNSETQKTINSLKKENE
                     ELKKQIASQSNDTEENSESIDNASKEKTLPVVGILEYTISSIKTEQVTNDEDNFTNAE
                     YNFSGIENFPEKYYRTTISYKLKNKATSPFSLSSYQASLIDGDGMEYSRTAGEYFLYD
                     ENSNSLVQPETTTSGVFYLLSKNKPNLSTFKLNASKQFTDDFTAEPIGEAGTTEYK"
     gene            complement(103066..103719)
                     /locus_tag="ACHA34_000318"
     CDS             complement(103066..103719)
                     /locus_tag="ACHA34_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8790999.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETNISKTKET
                     TEISSSTSKSTSKTDSSSTVTSSNQATAKPSPTVISSSQDTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNTEAQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(103769..104197)
                     /locus_tag="ACHA34_000319"
     CDS             complement(103769..104197)
                     /locus_tag="ACHA34_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8791000.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(104202..104576)
                     /locus_tag="ACHA34_000320"
     CDS             complement(104202..104576)
                     /locus_tag="ACHA34_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8791001.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            104879..105064
                     /locus_tag="ACHA34_000321"
     CDS             104879..105064
                     /locus_tag="ACHA34_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8791002.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALVGISGAYLSDILNGNRDG
                     KKAQEHIETVKKILGIQ"
     gene            105077..105415
                     /locus_tag="ACHA34_000322"
     CDS             105077..105415
                     /locus_tag="ACHA34_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFM8791003.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
BASE COUNT        34306 a        18023 c        22168 g        30930 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaactttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttttta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggcatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagaacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    44941 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    45001 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    45061 tttttacagc tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat
    45121 aaagaaagaa gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa
    45181 cgttgattat atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt
    45241 tgactcctta tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga
    45301 tatatgtacg accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg
    45361 aaaattcagg aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag
    45421 atttttatac aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa
    45481 atattatcgg tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg
    45541 catttgcatg gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag
    45601 catggaatac ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag
    45661 aatgttaaaa acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga
    45721 gaaaacactc atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg
    45781 agtagtatgc gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt
    45841 atgcgtaccc agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat
    45901 gtagcagaag taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga
    45961 cagcttcagc ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc
    46021 atggccacga caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg
    46081 atcgagaaag ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt
    46141 gaaggtcaag cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg
    46201 gatagtgcaa aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa
    46261 ggtggcgttt tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa
    46321 gagattcccc gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg
    46381 atggatcatc ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg
    46441 acaaaggaat cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg
    46501 attgaatcga gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta
    46561 aaggatacag gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc
    46621 aattgataac cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag
    46681 ttgggccaaa ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa
    46741 tttaattatc tataattatt gatgttccgc tgattcttgt catagtagct cagctggata
    46801 gagcatccgc cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa
    46861 ccgttagaaa ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt
    46921 ttcaaaggca aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa
    46981 ataaaactca tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact
    47041 ctagttgcaa ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac
    47101 acgacaagta gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca
    47161 ttgtttaaga accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct
    47221 tatctttctt ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt
    47281 gcattatatg gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct
    47341 ctgttggcat tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag
    47401 gaaactttgt gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt
    47461 ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa
    47521 aaaaagaatt ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca
    47581 cagtagctgc tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg
    47641 cggccgcagg tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg
    47701 gtttttatca aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg
    47761 caacaaaggg aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg
    47821 cagcaggact tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg
    47881 ggtttacttc aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg
    47941 ttcactcgga aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc
    48001 cgataaacac agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc
    48061 caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag
    48121 ttatgctatt gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca
    48181 gtagtaatcg tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc
    48241 cagctattag cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg
    48301 gccgaatggc aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa
    48361 atctgttgct tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg
    48421 tggccatcaa atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac
    48481 tgctctccat gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag
    48541 gtgttttgat tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta
    48601 ctaataaata ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct
    48661 ttgatttaag tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca
    48721 aaagcgctgc tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag
    48781 aaacggaaaa gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt
    48841 cttcagaaaa actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct
    48901 ccatgcgagg cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag
    48961 aaaaagcaga gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa
    49021 aacaactttc tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg
    49081 cgttgacagc tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac
    49141 agacaagact gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa
    49201 aagtgaagct agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa
    49261 aatagctgct ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc
    49321 atccttttgt tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt
    49381 aaccgtatat tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa
    49441 ttttggctgg accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga
    49501 acgagtaggt gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg
    49561 atgagtcacg cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt
    49621 taagccagtt gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac
    49681 atgcgtatca atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag
    49741 aggtccttgc ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc
    49801 ttgtatcagt tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat
    49861 aaaagaaaca caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt
    49921 attgcttgaa tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt
    49981 ctggtaagct agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta
    50041 ataagcacag catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc
    50101 tttatagcat gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac
    50161 aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag
    50221 ttatcttagt aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg
    50281 ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac
    50341 ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg
    50401 acgcgatcaa cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt
    50461 taagcgaaat gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg
    50521 tccttatgga agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa
    50581 cgggattgcc tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg
    50641 ttatctttat accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat
    50701 tctattagaa aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat
    50761 ccaaattaaa acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct
    50821 acaattattt gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc
    50881 aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta
    50941 ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac
    51001 tttatttgca aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca
    51061 agtgtctgtt tatccagtag ctcaagaagc ttaattgaat cttatgatta aagaggttgt
    51121 gacaagactt ttgtcacagc cttttttgtt tataaaaata aaagttgtag tggaggctac
    51181 gaagtttact ttgtcttttt acacttaata tttttttgcc aatttggaat caggatgttt
    51241 tgataactat cgttttcaat tgatggaagc tatattgttt gctatcagtg aaataattat
    51301 atggtaaaca gtgttctttt tcttgtttcc attataaaaa tcgtatcggg atttttcgtt
    51361 cttttaaatt gaacatttct atctatccgc gtgttatcaa caaaacaaaa tcaggtaaat
    51421 agttgacgaa acagaaataa atacatagta ttatgttttg aagcctaagt acaatagtct
    51481 atagaaaagg agcgatacca ttggcaaaac gcattgaccg aatttatatg tatgtcaaag
    51541 aaaaaaccgc tcatttaaca aaggcagaat atgatcaagg agtaacaaca caggaaattg
    51601 ctgaagtgtt agggattcag cgaacaaata gcagtaaaga tctgaatcag ctagtgcgcg
    51661 aaggaaaact gataaagaca gacggacgac cagtacgtta catatatcaa acactcgtta
    51721 cgcacgtaaa gccagttaca aaacatgtag tcagttacaa agaaacgccg gtctctgaag
    51781 aaaaaaatac actccaaata gatactaagg atatctttgc aaaaatcatt ggagctaacg
    51841 gcagcatgaa aaattcagtg gaacaggcaa aagctgcgat tttgtatccg ccaagaggat
    51901 taaattgttt gattacagga cctaccggat caggaaagac ttattttgca cacgcgatgt
    51961 atcattttgc aaaagcgcat catgtaatag atgaggaaac ggaactgatt gttttcaatt
    52021 gtgctgatta tgccaataac cctgagcttt tgatgagtca tttatttggg tacgcaaaag
    52081 gtgcttttac aggcgcagat gaagaaaaaa tggggattat cgatcaagcg gatggcggta
    52141 tgctgttttt ggatgagatt caccggttgc cgcctgaagg tcaagaaatg atcttttact
    52201 ttatggatca tggaacttac agtcgtcttg gggaaacaac gaaatcccat gaagcaaatg
    52261 tacggatcgt aggtgcgacc acagaagacc caggttcttc tctattagaa acttttgttc
    52321 ggcgtatccc aatcaatatc aaacttccgg cttttgaaaa acgtcctgca aatgagaaag
    52381 tcgatttagt aaaaatcatg attgcccacg aagcaaaccg cacgcagcga aagatttctt
    52441 tgacagaaga tgtagcaaaa gcattgatcg gcagtgtgac ctatggaaat ataggacaat
    52501 tgaagtcaaa tatccagtta gtatgtgcac gaggatttct aaatcatatg caatcacccg
    52561 aaatctcgat cacaatcgat gatttgacag aagggattcg tagcggattg attcaattag
    52621 caagcaaccg cagtgcaatg gcagagcttt ctaaaatatt ggagccaaag ataactgtag
    52681 caccaaatga tacgatcatg aaaatccaat cggactccta tgagttacct tataatctat
    52741 atgatatcat cggagataaa gcagctttac tgaagtcgga tggtttggat caagaagcaa
    52801 tcaatcattt catttctaca gatatcaaca tccatctgaa gtctttctat aaagaccatg
    52861 gtttttcttt taatgcagac agcaaattag cagaatttgt agatccgaaa atcatagaag
    52921 tgaccaatca gatttatgta atggtcaaaa gatctttacc atatgagttt cagcaaaact
    52981 ttatttatgc tatgagcttg catatcagtt cttttttgaa aaaaatcaac ttaggagaag
    53041 aacggcatac gaatgataac atccgtgaga tggcttttga ttttccagat gaatatgctg
    53101 ttgcgaaaga agtgcgtagt tatatcgaaa cgtattttca agtggagatt ccagaaagtg
    53161 aagactatta cttgaccgtt ctactcgtct cgttacgggc aaatcaagca agcggcagaa
    53221 tcggtgtagt cgttgcagct cacggaaaca gcacagcaag cagtatggtc caagttgtcc
    53281 aacagttgtt agatgcagag aacgtacgag cagtagacat gcctttggat atggatccga
    53341 aaacagcatt ggaccgaatt gaacgcaatg tacaagaagt agatgaagga agcggggtca
    53401 tactcttagt agatatgggg tcccttgcgt cgtttaattc gcaaattcaa agagacactg
    53461 gaattccagt acgtactgtt gatatggtaa caacttctct agttttggaa actgtacgaa
    53521 aggtctcagt attagggaca gatctagata tgttgcatga atcattgaaa aattttcgag
    53581 gttacgcaga aatatcttct gtagaatctg ttcaggaagc aaaacctatt cataagaaaa
    53641 aagctgtttt ggctatctgc gcatctggcg aaggtacagc acaaagaatc aaagaattga
    53701 tcgaacgagc ggtaaacaag cgtcaagaaa cagaattaga agttctagct ttgtcgattg
    53761 tggaagtgaa agaagaactg ccagttatcc aaaaagaata tcagatcata gctactactg
    53821 ggattactga tttgaatatc caagccccat ttattccttt agaacgtttt attgatcaaa
    53881 atatcgaagt cattttggat cagttgctga tggaatcaga attggaggaa actgaattca
    53941 tctccctaga tgaagagtca gctaaaaata cgtgtgttga gtttatctct gataacttca
    54001 tctttattaa tggtagtaag ttaatcgatc caatgtggca attttctaca caaatctctc
    54061 agacgacggg aatcggcgat gaagaatatg gattcaaaat caacctagtg atgcacacag
    54121 caggaatgat cgaacgtatt attcggaatg aaccattgac tgtggaagaa aacgaattga
    54181 caaatacaac gaatgatcct ctttatagcc aattggcagc ctcagttgtt ctgttggaag
    54241 atcagataaa agtaaaagtg ccgatagaag aaatgtatta tctgctacgt ctagtccata
    54301 atcagttaga taaaaaagaa tacactgtgc catgaataca caaaaacgat acacttttat
    54361 agtgtatcgt ttttgtgtat tggttaagaa tcctttaaat tcaattagtg tatcgtaaaa
    54421 aagttggcac gctttttgct tatagtagtg tgtgctagga ggtggttgtt cacacagaaa
    54481 tgataggggt ttctgtgaag tagtgaggaa gaaggagttt tgcagatgga tattcgattg
    54541 gcacgtatag atgatcgttt aatccacggg caagtcgcaa cagtttggtc gaagcaaaca
    54601 ggtgttgaac gtatcattgt gatcagcgat gtagtcgctc aagataaatt gagaaagttt
    54661 ctactaaaag aagctgcacc gccggggatc acagcaaatg taataaccgt gcagaaaatg
    54721 attcaaatct atcatagtgg attattatca cgagcaaaag tgatgctttt attcaccaat
    54781 ccccaagatg ttgaagcggt tgtccgctgc ggtgtacagt tgaagtcgtt gaatatcggc
    54841 ggtatgagtt ttactgaagg caaatcaatg attaccaatt ttgtttcagt aaatcaacaa
    54901 gatattgatt cctttcgtta cttgaacagt caagaagtgg agttagagat tcgaaaagta
    54961 cctgctgaca ggaaaatcct tttgatggaa ctactagcga aaatcaaaaa ggtgtagcaa
    55021 gaatggttgt tggaaaaggt caaataattc tgttgtaatg gttagcaatt attaatattt
    55081 ttgaatttgt tacaacaaaa gtaaatagga ggtataaata tggtagggat tatcctagca
    55141 agtcatggac aatttgctga aggaatctta caatccggtt caatgatctt tggcgaacaa
    55201 gaaaacgtca aagccgtgat tttgaaacca agtgaaggtc cagatgactt gagagccaaa
    55261 ttggaagaag cggttgcttc ttttgataat caagacgaag tgttattctt agttgattta
    55321 tggggcggaa caccgttcaa tcaatcaaac acattattcg aagagcacaa agataaatgg
    55381 gcaatcgtca gcggtttgaa tttaccaatg ttgattgaag cctacgcatc acgcttttca
    55441 atggaatctg ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga cggtgtaaaa
    55501 gtaaaaccgg aagaattaga accggcagaa gcaccgaaag cagcagttga agacgcacaa
    55561 cctaaaggag cacttccaga aggaactgtg gtcggagacg gaaaaatcaa atatgtatta
    55621 gcacgtgttg actcacgtct gttacatgga caagttgcga cagcttggac aaaagcagtg
    55681 caaccaaacc gtattatcgt tgtttcagat gcagtatcaa aggatgatct gcgtaagaga
    55741 ctaatcgaac aagctgctcc tccaggtgta aaagcaaatg ttattcctat tagcaaaatg
    55801 atcgaagtag caaaagaccc tcgttttgga aataccaaag cattgctttt atttgaaaat
    55861 cctgaagacg tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt aaatgttggg
    55921 tcaatggcgc attcggtcgg taaagtcgtt gtaagtaaag tattgtctat gggacaagaa
    55981 gacgttgaag cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt acgtaaagta
    56041 cctaatgatt cacgcgataa catggatgat attttgaaaa aagcaaaagc tgaattagca
    56101 aaagcgtaat aaataatatt aaaatttata ggaggcttat catgtctatt atttcaataa
    56161 ttttagtcgt attagttgcc tttctagctg gtatggaagg tattcttgat gaatttcaat
    56221 ttcatcaacc actagtggca tgtacattga tcggtttagt taccggtaac ctcgaagctg
    56281 gtatcgtact tggcggtacg cttcagatga tcgctttagg ttgggcaaat atcggtgccg
    56341 ctgtagcacc agatgctgca ttagcatcag tagcatcagc aattatttta gttttaggtg
    56401 gacaaggtgt taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg gctgttgcag
    56461 ggttgttctt aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat ttgatggatg
    56521 cggctgctga agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt gctgtatgtt
    56581 tacaaggggt tcgtatcgcg gtcccagcag cagcgttgct atttatccca gcacaaactg
    56641 tgcaatcatt ccttgaatca atgccagcat ggttaacaga tggtatggct atcggtgggg
    56701 gaatggtcgt agctgtaggt tacgctttgg taatcaacat gatggctaca aaagaagtat
    56761 ggccattctt cgttattggt ttcgttgtag ctgcaatttc tcaattaaca ttgattgctc
    56821 taggtgcttt aggtgttgca ttagctctta tttacttgaa cctttctaaa atgggtggtt
    56881 catcaaataa tggaggcgga agcaactctg gtgacccttt aggcgacatt ttgaatgact
    56941 attaatcttg aaggaggaaa ataaaatggc agaagaaaaa atcaaattat caaaaaaaga
    57001 tcgtttagct gttgcgtggc gctctacctt cattcaaggt tcatggaact acgaacgtat
    57061 gcaaaacggt ggctgggcat tttctatgat tccagcaatc aaaaaattat acaaaacaaa
    57121 agaagaacgt tctgcagcat taaaacgtca cttggaattc tttaatacac acccatacat
    57181 tgcttctcca attttagggg ttacattggc tctggaagaa gaacgtgcga atggcgcgcc
    57241 tgttgatgat gtagcgattc aaggggttaa agtaggtatg atgggtcctt tagctggtgt
    57301 tggtgaccca gtgttctggt ttacagtacg tccaatgcta ggtgcactag gtgcttcatt
    57361 agctatggga ggaaatatcc ttggaccaat catcttcttc ttaggttgga acattattcg
    57421 ttgggcattc atgtggtata cacaagaatt tggctacaaa gctggttcta aaattactga
    57481 tgacctttct ggcggattgt tacaagacgt aacaaaaggt gcttcaattt tagggatgtt
    57541 cgtattagct gcccttgtac aaagatgggt atcaatcaaa ttcttaccag ttgtatcaac
    57601 tgtaaaatta gataaaggtg cttacatcga atgggataaa ttgccttcag gtggtgaagg
    57661 aatcaagagt gcatttgaac aagttaataa tggcttagcc ctttctcctg aaaaggtaac
    57721 aacattacaa gacaacttag accaattgat tcctggttta gctgcattag gattaacatt
    57781 gttatgtatg tggttgctta agaaaaaggt ttctccaatc gttatcatcc ttggtttatt
    57841 cgtagtcggt gttgttggac acgtcatcgg acttttataa tccataaaaa tatagctcgg
    57901 gcgttgtccc gagctttttt tatatagtat actgttcata aacaaatatc tggagggaga
    57961 aaatggttca atcaatcaat acaaaagtag acttggtaat tgatgctact tcgtttacgg
    58021 gtctgacaga ttacgggcaa atcatgatag gtgataatgg ttttgagttt taccatgcgc
    58081 gagatagacg caaatatatc caaattccat gggaagaagt ggattatgta ctagcatcag
    58141 tcatgctaaa gggaaaatgg attcctcgat atgccattca aacgaaaaaa aatggtacat
    58201 acacattttc ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa tatgtagatg
    58261 aagatagaat ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt attcgtagta
    58321 tgtttaaacg caagtaaaaa ataaagaaaa cttctaagag gaggttttct ttattttttt
    58381 actttgcaga agaaaatatg aaatagcaaa accaattatt tgtccccttt aaatcccata
    58441 tgctaaaatt gattagcaaa gaaggaggtt atattatgag ttggttatgg acattgattg
    58501 ttggagccgt gatcggagca attgcaggtg cgatcaccag taaaggaaat tcgatgggct
    58561 gtgtaacgaa tatcattgct ggactagtcg gttcaagtat tggacaagcg attttcggcg
    58621 aatggggacc aaaagctgca ggtatggcac ttattccatc tattttaggc gcagtgattt
    58681 tggtagcagt tgtctcattt ttctttggaa aacgatcata agtttttaca aaatagtaaa
    58741 ctaaaaggag ttgcagcgag actgtgactc ctttttggct ttatttattt aaaataaaaa
    58801 gaagttttcc ggaaattttt atttaactgt cgtgtacgaa gaaaagtttg ctataatgga
    58861 aaaaagcgtg caaagaaaga gggagtttca ttatgatgct acgtgtagaa aaattgcgca
    58921 aaaaaatgca agaagaaaat ttggattcat ttttggttac cagtccgtac aatcttcgtt
    58981 acttaacgaa ctttacaggt acgacaggat tagctgttat tactttggaa aaagcatttt
    59041 ttattactga ttttcgttat accgagcagg cggcagcgca ggctcaaggg tttgaaatta
    59101 ttaaaaatgt cggacctatt tttgatgagg tagcaaactt agtacgcaaa gaagaactgc
    59161 actcattagc ttttgaggaa acaacagtct catttttaga atattcggtt ttagaagaaa
    59221 tcatcgatgc ggaattagta cctgtttcac agatgatcga agaactcaga gaagtgaaag
    59281 atgaagaaga aatcgctatt atcgaaaaag catgtcatat tgcggatatg gcatatgatc
    59341 acatcctgaa aatgatccaa ccgggcatga ctgagattga agtcgcaaat caactagact
    59401 tttatatgcg ttctcttggc gcttcaggtg tttcttttga tacgatcgtt gctagcggat
    59461 tgcgttcggc tatgccgcat ggtgtagcaa gcaaaaaaat catcgaacaa ggtgacttga
    59521 tcacattgga ctttggttgc tattacgaag ggtacgtttc cgatatgaca cgtacattcg
    59581 ctattggtga tcctggggaa aaattaaaag aaatttatca aatagtctta gatgctcaat
    59641 tagcagttat tgatgctgca aaaccaggaa tgactggtgt ccaattagat gcagttgcta
    59701 gagattatat ttcaaaacat ggttacgggg aagcatttgg acattctaca ggacatggga
    59761 tcggtttaga gatccacgaa ggaccaaacg tttcttctcg tgcagaaaag caatttgtac
    59821 caggaaatgt gatcactgat gaaccaggga tctacttacc aggaatcggt ggagtacgta
    59881 ttgaggatga cttgttgatc acagaagatg gaaatcgtgt gttgactcgt tcaccaaaag
    59941 aattgatcat cttatagttt catcaataga agaagtaagc gtaaaaatca gtgttcacag
    60001 actttgttat agaaaatcac tcgatttaat gatacactat agaagaactt ttaagctgta
    60061 agagataata aagttgggtg ttttctccaa ttcatagcag tttgcagctg atcggcggca
    60121 gttttcttac cacgtgatca ctatgccgca tcatagagaa ttcggatgta tctcgtgctt
    60181 aggcaaaggg aggaataaat aaatggcaga tgaaaaaaat ttgattttaa acgcaaacca
    60241 agatttagga gagatcgtga tcgctcctga agtcatcgaa gtaattatcg ggattgcagc
    60301 ttctaaagta gaaggcgtat acggaatgcg cggaactttt gccagcaatg tcactgaatt
    60361 attaggacgt gcagctcacg gaaaaggtgt ttacttagca aacgaggaag atggcttgaa
    60421 agtagatctt tactgttatc tagaatatgg tgtttcagtt cctaaagtag caatggaaat
    60481 gcaggaacgg gtcagccagc aagtcctttt catgacggat atcgatttat ctgaagtcaa
    60541 tatccatgta gtagctgttg ttcctgaaaa attagaacaa ccaacaatcg aagaactttt
    60601 tgaagacgaa gaggaagaga atgaataaag aattatcaag acacgagatt cgggaaatgg
    60661 cattgcaggc tttatttcca ttagacttca atgcagacct aacaaaagaa gatgcgattt
    60721 tcaacgcaat cgaattagac caccgcgaca tgatcaacga agatgaatca gagtttgttc
    60781 ctgtttatct tgatacgcta gttggcggtg tctgtgcaaa aaaagatgaa ttggataaag
    60841 tgattgaaaa acatttaaaa aacaattggc acatcaatcg gatctcaaaa atggatttgg
    60901 tgatcttgcg tatcgcgatt tttgaaatga cttatgtgac agatgtacca gcggctgttg
    60961 ctttaaatga agcaatcgag ttggcaaaaa catttagtga tgaccgttca cgtaaattcg
    61021 tcaacggtgt tttatccaat gtattgaaag aattagaagc tggggcgtaa gtcctagctt
    61081 ttttctatga atcatcataa acctgttata aatcgggaaa attctatggt agaatgaaaa
    61141 cacaaaaaat atctgggagt ggttagatgg cagaattgat taacggaaaa gaattagctg
    61201 aaaaaatgca ggcagagatt gctgaaaaaa tccaaaaatt gaaagataat ggtatccatc
    61261 ccggattagt cgttttattg gtaggggaaa atccagccag ccaaatctat gtaagaaata
    61321 aagaacgtgt agcaaaagaa atcggaatcc attcattggt agagcgttat cctgagacga
    61381 tttctgaaga cgaactactt gcagaaattg aaaaatacaa tcaagatcct agttttcatg
    61441 gtatattggt ccaattacca ttgccaaagc atatagatga agaaaagatc ctgttagcta
    61501 tccgccctga aaaagacgta gacggttttc atccgatgaa tttaggcaga ctccttgcgg
    61561 gtaaaccgga caagattcct tgtactccat acggcatcat gaaaatgttt gaagcttatt
    61621 ctatcgatcc ggcgggtaaa cgagcagtag ttatcggacg aagcaatatc gtcggtaaac
    61681 cgatggctca gctactcttg atggcagatg caactgttac tgttgctcat tctaaaaccg
    61741 aaaatctggc tgaactggca caagaagcag atattttagt cgtagcaata ggacgtggac
    61801 attttgtcac aaaagaattc gtaaaaccag gtgctgtagt gatagatgta gggatgaatc
    61861 gtgatgctaa cggtaagctg atcggtgatg tgaaatttga tgaagtggag cctattgcag
    61921 gctggatcac tcctgtgcca aagggtgtag ggccaatgac tatcacgatg ctgatgtatc
    61981 aaaccgtaaa atctgccgaa gcttttggag caggtgaata gatggcacaa gaatatttaa
    62041 ctgtcactac attaacgaag tatttgaaaa gaaaatttga tgccgatcct tatttagaac
    62101 gcgtatattt aacaggcgaa atatcgaatt tccgtttacg tgccaatgcc catcaatatt
    62161 tcagcttaaa agatgaccat gcaaaaatat ctgctattat gttcaaagga gcattccaaa
    62221 aattacgatt ccagccaaaa gaaggaatga aagtccttgt cgttgggcgt atttcattat
    62281 atgaagcggg aggttcttat caaatctatg tagagcacat ggagcctgat ggaataggtg
    62341 ctttatatca agaattggaa gaacgaaaag aaaagctgat gaaggaaggc ctttttcaag
    62401 ggcctaaaaa aatgctgcca cgtttcccaa agcgaatcgc ggtccttacc agtccaagcg
    62461 gagcggttat ccgcgatatc atcacgacgg tgaaaagaag atatccaatt gtccaattgg
    62521 ttttgttccc aacagttgta caaggaaacc aagcagcgga caatgttgtc aggaatatcc
    62581 agagagtaga agagatgggc aatttcgata caatgatcat cggtcgtggg ggtggttcga
    62641 ttgaggattt atggccgttt aatgaagagc gggttgctcg ggcgatttat caagctaata
    62701 cgccggtgat ttcatctgta ggacatgaaa cagataccac gatcgctgat ttagtagcag
    62761 atgttcgggc tgccactcca acagcagctg cagaattagc tgttcctgtt ttgtcagaag
    62821 agttgatgcg cattcaagaa cggcaagcta gattgcagca ggcgtttgta cgccagatcc
    62881 agcgcaaaca agaacggttc gagcgtgctc gacgttccta tgtatttcgg cagcctgaaa
    62941 gattgtatga aggtcagacc atcaaattag accagttgaa acagcgatta tatcaagcta
    63001 cacagcaaat ttatcatgcg aaagaaaaac aaacgatcgc attaagccac caattggaac
    63061 aagtggctcc gctttatcga gttaaagcag ctcgccaaga aactgcttat ctagaaaaac
    63121 gcttagaaga gaaaatccaa caatatatga aacaacagca gcagcgtttt gctcaagctg
    63181 ttcaatctct tgatttgttg agtccattga aaatcatggg aagaggctac agctatacga
    63241 cgttaaatga tcaagtagta aaaagtgtca ccgagattca tcaaggggat ttgctgacga
    63301 tccattatca agatggaatc gttaaaggac aagtacaaga gatagacaaa ggagaataaa
    63361 aatatggaaa atccaacttt tgaagagtca ctgcaagaat tagaaaaaat tgtcatgcag
    63421 ttagaacaag gagacgttcc attagaatca gctctagatt cgtttaaacg aggaatggaa
    63481 ttaagcaaac attgccaaga tacattagct aaagcagaag agaccttgac gaaaatgatg
    63541 acagaatcaa atgaagaagt tgattttgac ggaaacgagg aaagctgaga tgagattaac
    63601 tgacttttca gctattcatc tacctgctgt agaaaaagaa atcttgtctt ttttagatga
    63661 gcatacaacg gatagaggac tctatgatgc gatgagttat tcagtgaaag caggcgggaa
    63721 aagaatacgt cccttattac ttttaacggc cgttgcttcc tttgatgagc ctattgatgt
    63781 tcccgtctat caagtagcag ctgctttaga aatggtgcat acgtattcct tgatccacga
    63841 tgatttgcct gcgatggaca atgatgatct tcgtagagga aaacctacca atcataaagt
    63901 attcggcgaa gcattagcta tattggccgg agatggtttg cttacaggag cattccagct
    63961 aatcagtatg gcacacttag gtaattctcc taagcttctg ctgcttcagc aattagctgt
    64021 ttgtgcggga tcacaaggta tggttgctgg gcaagctgca gatatcgagg gcgaatcgaa
    64081 aaagctttct ttagaagaat tagcatttat tcatgaaaga aagacagggc atttgattcg
    64141 ttatgccttg ctggctggtg gcatattagc gaagcaacct gaagaaatct tattgctact
    64201 tcaacgatta gccgaacatt taggtttagc ttttcagata agagatgatc tactcgatgt
    64261 gattggtaca acgaaaacat tagggaaaac agctggtaaa gatgaacgta tggaaaaaaa
    64321 tacttacccg cgtttattag gcctagaaaa gacacgtgag gcgttggaga ttgagctgtt
    64381 atccgccaac aagatcatag acaaattaga agaggacgtt ttttcttttg atggcgggct
    64441 gatgcgccag atgatcaagc aatttgatgt ggagaaatga gggaaagaat ggaaaaagaa
    64501 cgcgtagatg tattagcggt caaacaagga ttatttgaaa caagagaaca agccaaacgt
    64561 tcagtgatgg ctggtttgat ttacaacgaa aaaaacgagc ggtttgataa accgggagaa
    64621 aaaattccag tgagtagtga attaagagtc aaaggtaaaa aacttcctta tgtttctcga
    64681 gggggattaa aattagaaaa agcactcaaa gcatttgatc tatctgttga aggaaaaaca
    64741 ttgttagata ttggtgcatc cactggcgga tttactgatg cagctttgca aaatggcgca
    64801 aaaatgagtt atgctttaga cgttggctac aatcagctag cttggaaaat aagacaagat
    64861 cctagagtcg tcgtaatgga aagagtgaat tttcgttatg caaaacccga agactttacc
    64921 ttaggaattc cagaagttgc tgttatcgat gtttctttta tttcactaaa actgatgctg
    64981 cctccattac atgctatttt aaaagaagag ggagaagtga ttgctttaat caaaccccaa
    65041 ttcgaagcag gcagagaagc tgtagggaaa aacggaattg ttcgtgaccc tcagacccat
    65101 caaaaagttt tagaagacat tttgtcattt gcggcacaag gtggatatga tgtgctggaa
    65161 ttaagttatt ctccgatcac ggggggagaa ggaaatatcg agttcttggc tcacttgagg
    65221 aaagtacccg aaagcggaac aatcaattca gctattaata tggcagaagt ggtttcaaat
    65281 gcacacgaac agtttgacca taaataaatt gtaactgtga aaatcaagtg agaagtatgc
    65341 ggattcttct ttattatacc aaggaaataa gctatcattg attaagaaga atatgctaag
    65401 gagcgaagac gtaatgagaa aaaaagatcg gcatcgtttg attactcgat tgttgagtga
    65461 gcaagatatc cgaaagcagg aagaatttgt tgaaatcctg aaaagcaaag gaatatctgt
    65521 tactcaagct acgatttcca gagatataaa ggaactgaaa ctgatcaagg taccggcttc
    65581 agatggcggc taccgttata gcataccagc tgaaacaaac gaagatgtaa atactaaatt
    65641 ggaaaaacta ttaaaagacg ctttcgtggc agttgaccaa atggaaaaat ttgttatttt
    65701 gaaaacattg ccgggaaatg cttcggcagc agcaaatttg attgataaac gttacaagaa
    65761 agaattattt tcgattatca atgatgacga taatgttttg atgattacac gaactgaaga
    65821 ccaggcagtt ttattgaaga aagacttcct gcattacctt taagttgagg agcgaatgtg
    65881 atgctacaag aaatcagtat tactaacttt gcgattattc cggaattgcg gctttctttt
    65941 cacgaaggaa tgaccgcgct gaccggagag actggtgcgg gtaaatccat tattatcgat
    66001 gctttaggtc tgttagcagg aggaagaggt tcaagcgatt atatacgtca aggagcagaa
    66061 aaatgtgtgc ttgaagggtt gtttgaacta ccaaaacaag aaggattctc tgaactgatg
    66121 gttgaattag ggatcgaaac agatgaggac aatctgattg ttcgacggga tatgtcctta
    66181 actggaaaaa atgtttgtcg agtgaatggt catattatca cattagctaa tctgagaaga
    66241 atcggcagtt atttagtaga tatccaagga caaaacgaac atcaggaact gctccagcct
    66301 gaatcacatc ttgctttatt ggatcgtttt ggagatgtag ctttccaaca gaagaaaaag
    66361 agttatcagc aagaatatat ctcttatcgt gagctggaaa aaagggtacg aaaagtgcag
    66421 caaaatgaaa aatcctatgt tcagagaata gatatgctcc atttccagca agaagaaata
    66481 gctgctgctg atttgcaagt cggagaagaa gagaaattaa aagaagaaag agaaaaactt
    66541 agcaattatc aaaaaattgc tgatggatta gctgcaggtt acggagcact gacgaataat
    66601 gaacaaaaca gtgtagatgg tgttggcttg gccgtttcag aactgcaagg gatcgcccat
    66661 ctagatgtgg agtatgaagc aatttatgaa aatatccaaa gtgcttacta tttgctgcaa
    66721 gatgcaatcg gcgatatgag tcgtcagatt gatttattgg agttagatga gagtcgttta
    66781 gaagaagtta cgcaacgttt agaattaatc cgacagttga aacgtaaata cggcgaatcc
    66841 attgagtcaa tattggctta ctatgacgaa atcaccgaag agctagcttc ttctgatttt
    66901 tcagaaggtc aattggacaa aatgaaagag gaactagagc aaaaagagct tctacttcag
    66961 caacaagccg cagatcttca tgaagcgcgt aaaaaaatcg caaaagaatt ggaaaaatcc
    67021 attctgcatg aattgaaaag tctgtatatg gaaaacactg agtttgaggt acgtttctta
    67081 aaagaagaaa atagacaatt gaatcatgat ggatttgacc aaatcgaatt ttacattacg
    67141 accaatccag gagagccact gaagcctttg gtgaaagtcg cttctggtgg cgaactgtct
    67201 cggatgttgc ttgctttgaa aactattttt tcttctgagc aaggtgtgac aagtattatt
    67261 tttgatgaag tggatacagg tgtgagcgga agagttgcac aggccatcgc agataaaatt
    67321 tctcaaatat cgaaatactc tcaagttttg tgcatcactc atctgcctca agttgctgca
    67381 gtcgccgact atcaatatta tattgtgaaa gcagtgattg atggacgtac gcaaacttct
    67441 gtttcagagt tgaaaacaaa agaaagagaa gaagaaattg cacgtatgct ggctggaagt
    67501 gagattacaa aactcacact cgaacatgca aaagaactat tgaaactcgc aaaaaggacc
    67561 tgagccacct cgcagtggtt caggtccttt tcagcagctg atagctatat tttattttta
    67621 catgacactc attacagcag tcaatacaac agatccaatc gttaaaatca gcattgtcca
    67681 aatgacaaat tttgttactt tagcaaaagt acttgtttgt tttttttcac gcattgtttt
    67741 caacctcgtc attaattagt tgattcaagt gtacaatctt ttttccattt ccacaagagg
    67801 gcttttttat aaaaacttcc ctttttttaa ataataggta gttacgatac aaataaataa
    67861 tgttaataga actagccata taaaggcatc atccctttca gcaataggta acttaatgtt
    67921 cattccatag atactgccga cgattgtcgg tatagttaaa ataatcgtta aagaagttaa
    67981 aattttcata atattattca gattattcga tacgattgct gaaaaagtct gattcatttg
    68041 ttctactagt tttaattgaa tgcgagaagt agtcattgcc tgctctgttt ccaccaatac
    68101 atcacgtaaa tgatttttca aagcatgatt ttcttggaaa agtttatttt gcgctagttc
    68161 tttcaatgca tctaaattag cagaagtcgc ttctctgaaa taaacgaggc ttttttggat
    68221 atccatgatt tgataaagtt gtttgttttc agtggatact tgaagtttgg cttccaaagt
    68281 atcgcattga gattgaagcg tagctaaata actgttgtaa gagagtgtga cctgccaaat
    68341 gaattgaaag aagatattga gattaggcga aatatcactt tttggaaagg tctggctaaa
    68401 gactttcttt aaaaacagtg gttcatgatt cgtaattgtg ataagcttct ttttgggcgt
    68461 caaaataata gagagcgggt atgtatccac ttgaaaataa ccgctaggac ttgttttagc
    68521 aaaaggatat tgtaacaata ataacgctgg ctttgtcaac tttgattgat tgaattcttc
    68581 tgtacgggaa ttttctgcat cgtccaatat ggaagtgatg taatctgacg gaatttcaaa
    68641 ttgatgtgtc agctgatgaa tctcttcttg attaggacgt tctacacaat accagatcgt
    68701 ttggtcagtt tcctttccgg tattacgcag tttctcatta tctaatgtaa aatatttgat
    68761 catgattttc ctcctgttgc ctgtttcttt tattataacg gatagggaat tggaatacat
    68821 ggaatgtgtt tgtaagatat agaatataac tgcataaatt taaataaaat gaaacagttg
    68881 tgtaaagtat atacagtata cttgaagcaa cgataaaaaa ggagtttacg ttcatgggca
    68941 cattcacacc acttatcttg ttttggtata tttacccggt aatcgttttg tttggttgcc
    69001 aatttcttgt aaaattattt tcgttaaatc gtcgttttaa aataaaagcc cctgatttgg
    69061 cgattccttt tttgtggggc ggtatgcatg cattgtctag aaatacaggg acaatttcta
    69121 tcttaccgtt tttctttatt tctgttttgc taatgggaat ttgtattgct gttttccaag
    69181 catattatta tgaagaaatt atttatccgc gttatttcaa gatgacttgg cgtttggttt
    69241 ttctgttgac aatcgtcttg tacgctgtct tgatcattgt gaacatactt tcttacctat
    69301 gaaaaaaata gaaaagctgt aaaaagtctt ttaaaaggct ttttgcagct tgatttttaa
    69361 aaaaatgttt atgaataaaa aaagttgttc ccggatttcc cagaaaaggt ggtagaaagt
    69421 gggggattgt ggtagactaa ttttagttag tggaggaatg gggggctttc aatgttgatg
    69481 ggtgaattcc aacataatat tgatgcaaaa ggacgtttaa tcgtgccctc aaaacttcgt
    69541 gaagaacttg gcgaaaagtt tgttctgaca cgcggattgg atggctgtct tttcggctat
    69601 ccgatgagcg aatgggaaaa tctggaagct aaactgaacg aaatgccgtt ggctaaaaaa
    69661 gacgcacgta cttttgtgcg attcttctat tcggctgcca ctgaatgcga acttgataaa
    69721 caaggacgaa tcaatatacc cagcacgtta agaaattatg cagctttgac aaaaggttgc
    69781 gtagtcatcg gtgtatctaa ccgaattgaa atttgggatg agacacgatg gcaagatttt
    69841 tctgcagcag cagaagagaa ctttgacgaa attgctgaaa gtatgattga tttcggacta
    69901 taagaatagg aagaggctga ctaatgactg aaacatttca acactataca gtcatgttga
    69961 aagaaacggt agatggattg aacatcaaac cggatggcgt ttatgtagac tgtacactcg
    70021 gcggtgccgg acatagtgaa tatttattaa gccaactaag ttctaaaggg catctgtatg
    70081 cgtttgacca agatcaaaaa gcaatcgatc acgccaagct gcgtttagct tcttatgtcg
    70141 aacaaggtat ggtcacattt atcaaagcga acttcagaga attagaagaa cgcttgagtg
    70201 agcatgtaga taaggtagac ggcattttgt atgatctagg ggtttcctct cctcagctag
    70261 atgaagcaga acgtggattt agttatcatc aagatgcacc gttggatatg cgcatggatc
    70321 agtcagctcc tttatccgct tatcacgtaa ttaatgaata ttcctaccat gaattagtaa
    70381 agattttctt ccgctatggc gaggagaaat tttccaaaca aattgctcgt gaaatcgaac
    70441 gagtacgaaa aacaaaacca atccaaacaa ctggtgagct tgtcgaaatc attaaacaag
    70501 tcatcccagc tcccgcaaga agaaaaggcg ggcatcctgc aaaaagaatt tttcaagctg
    70561 tgcggattgc agtaaatgac gaactcggtg ccgaagaggc ctctttagaa caggcaatcc
    70621 ggttattgaa agtcaatgga agaatcagcg taatcacatt ccattcattg gaagacagaa
    70681 ttgtcaaaag tatgtttaaa gaatatagta cggtacaaga tctaccaccg ggacttcctg
    70741 tcatacctga cgaattccaa ccagaactga aagtcattac tcgtaaacca atccttcctt
    70801 cagaggaaga gctaacagaa aacaatcgat cacgcagtgc aaagttacgt attgcagaaa
    70861 aaataagaat gaaagaggag tagaatgatg gcagaattaa agaaaatgca agattacgaa
    70921 tatgatttac ctgaattggt ggatcagccg gaacaacaaa tgcctgattc ttcttcgaat
    70981 caagaaatca ttcttccaca atcaccggca aaacgattaa agcagatttc tgctttggaa
    71041 aaaattgctg tggcaagcgt ggcgattgct attgttgctt tatgtgtttt aacagttatg
    71101 ctgcgcacaa atatcagcgg agctgaaaaa gaaatcacgt caatacaaac agaaatcaat
    71161 gataaaaagc aagaaaaaac gagtttagaa caagaaaaaa acgaactttc tcgtactgaa
    71221 cggattaaac aaatcgcaga agaaaaagga ttgtctatta atgacgacaa tttaaggaaa
    71281 gtgaagtaaa tgagtcttaa aaataaaatt cggcggttta tggaaaagaa aaatctaaat
    71341 ccgatgaaca atcgcaaaaa agtaggtatc attttatttg ctacgagtat tggattgttc
    71401 tttttatttg ccgtccgttt ctcgtatatc gttattggag gtcatgttgc tggtacctca
    71461 ttagctgaaa aaacaaaaca gttgtaccaa ggaagtgaag tagtaaaagc aaaaagagga
    71521 acgatttatg atcgtaacgg tgtggcgctc gcagaagatg cctcctctta ttcaattaaa
    71581 gcgatactct caaaaacata tacctctggg gataaaaaac tgtatgttga agaaaagaat
    71641 tttgataaaa tagcagaaat cctgcataag aatctttcta tagacaaaaa agatgcactg
    71701 aatattttag aggacggcgc aaaaaaagag ctgtatcaag ttgaatttgg tagctatggg
    71761 aaaaatatct cccaagaaac caagcaaaac atcgaagcgg acatgaaaaa agaaggtgtc
    71821 gctggtcttt attttgtcga ccatcaggct cggatgtatc caaatggcgt cttttcttct
    71881 cattttattg gatacgcagt tccagacaaa gatgagaatg gcttagttgg taaactaggt
    71941 ttggagtctg cttacaatga tatcttgagc ggtaaagatg gtaagattat ttatcaaaaa
    72001 gataattttc aaaatcctct gccaggtaca gtggcagaag aagaaaaagc tgtcgacgga
    72061 caagatatct acacgacttt agacagcaga ctacaaagtt atttagaaac actgatggat
    72121 caagtaaatg aagaatatca gccagaagaa ttgacagcag tcctgatgaa agcaaaaacc
    72181 ggagaaatct tggcaatggg acaacgacct actttcaatc ctgaaacaat ggaaggattg
    72241 actggaaaag atgctatctg gcgtaatttc ttggtacaag atagttatga accaggatca
    72301 accatgaaag ttttcactac tgcagctgct atcgaagaag gagaattcaa cgagaatgaa
    72361 accttccaat ctggaaaaat ccaagtagaa gatgctacga tcaatgacca tgactttggt
    72421 gaaaaaggtg tgctgaccat gcgtcaagct ctttcatggt caagtaacgt cgggatggtc
    72481 atactggaac agcgattagg cggacgatgg tataactatt tacaaaaact gggatttgga
    72541 caaagtactc attctggatt agatgatgaa gtaaacggtg ctttacctac attaaatatt
    72601 gtcgaccgag caatgagtgc ttatggacaa gcggtaggtg tgacgaattt ccaaatgatg
    72661 aaagctttca cttctattgc gaataacggt acaatgattc aacctcgata tatcagcaaa
    72721 gtggtggatc cccaaactgg agaagagcga acaactcaaa cagaagttct agggcaacct
    72781 ttttcaaaag aaacaacaga aaaagtccgt gaatatatga gagatgttgt cgaaagcgaa
    72841 aactacggta gtgcttatgg tgtatatagt gtacctggat acaatgtatc ggcaaaaaac
    72901 ggaacagccc aaatcgcttc tgatacaggg ggatatcaaa caggagatac agcctacctg
    72961 tattcgattg tggagatggt tccttctgaa gatccagact atgtcttgta tctaacaatg
    73021 aaacacccta agacatatga tcgaatggca ttggctaaaa tcgcaaatcc attgatgaaa
    73081 cgggcaatgg attttaaaga aactgaagaa gattcagata cagaaacaaa aacagaaaaa
    73141 gtatccgtag ccgattatcg taatcttgaa gcggacgttg cagcagctga tgctcaaaaa
    73201 agtggactgc aacctgtagt gatcggtaat ggtaaaaaag tacaaaaaca atctactgcc
    73261 aacggtgatc agttgatctc tggagaaaaa ctgatcctat atactggtgg tgataagctg
    73321 atgcctgacg taacaggatg gtcaaaagca gatatcatga agctggggaa aatcctcggt
    73381 attgaagtta gttttgatgg agatggttat tgtgtcaaac aagaacttgc tccatatgaa
    73441 aaaatcactg aagacaaact tagttttact ttagaagaat aagaagagaa ataggagaga
    73501 aaagagcatg gaatggacac aagcattgat tcccattgtc agcagttgtg caatgacaat
    73561 tgccgcaatg ccgctattta ttggttattt tcaaatgaaa aagcaaggtc aggcgattcg
    73621 tgaggaggga ccaaagtggc acaatagcaa agccggaact cctacaatgg gcggcttagt
    73681 gtttctgatc ggctctattt tgacaggaat ttgggttgga gcttggcaaa aacagctaac
    73741 tcctactctt ttcatcttgc tttttgtatt agcgttatac ggagtaatcg gctttcttga
    73801 tgattttata aaaatattca agaaaagaaa catgggactg aactcgaagc agaaattgat
    73861 tggacagatt ctagggggaa tcatctttta tcttgtttac cgttctgaag gttatccagg
    73921 tgtattgaat ttcttcggac tagatctacc tttaggaatc atttatggta ttttcgctct
    73981 cttctggctt gttggatttt caaatgcagt aaatctgaca gatggcatcg atggattagt
    74041 tgctggctta ggaagtatct cttttgcagc ttacggtcta attgcttggc atcagcatca
    74101 gtatgatgta ttaatcattt gcttaagtgt tttaggaggt ttactgggtt tcttcgtcta
    74161 caacagaaaa cctgccaaga tttttatggg agatgtcggt tcattagcgt tgggcggact
    74221 tttagcggca atttctataa tgctaaacca agaatggaca ttgcttttga ttggtttgat
    74281 ctatgttatg gaaacagcaa gtgtgatgct tcaagttact tcctttaagc ttacaggaaa
    74341 acgaattttt aaaatgtctc caattcatca tcactttgaa atgtgcggat ggtcggagtg
    74401 gaagattgat acagtttttt ggttgatcag cattgtgact tcgttgatca cattatggat
    74461 cgtttggtag gagatgacgg gaaatgaaaa aaatatcaac atatgaaaat aagaaagtgc
    74521 ttgtcttagg actcgcaaaa agcggagtaa gtgcggccaa attattacat gaattgggcg
    74581 cacttgtcac cgttaatgat ggaaaacctt ttgacgaaaa tccggaagca caagagctgc
    74641 tttctttagg gataaaagta ataacaggta gtcatccaat cgaattgttg gatgaagaat
    74701 tttcattgat ggtcaaaaat ccagggattc cctacagtca tcctttagta gcaaaagccc
    74761 aagaaatggg tattccggta attacggaag ttgagctagc ttacgaagta gcggaatgcc
    74821 caattattgg gattacagga acgaacggaa aaacaacgac aacaacgatg acaggactac
    74881 tactgaatgc aggagcagat caaggaattg cacgtttagc gggtaatatt gggtatccag
    74941 caagtggtgt agcgcaagaa gcaaaatcag aagataaaat tgtgatggaa ttgtctagct
    75001 tccaactcat gggaatcaca gatttccgcc ctcatatagc ggtgattacc aatatctatg
    75061 aagcacatat tgattatcat ggtacaagaa aagaatatgt caaagctaaa tggaaccttc
    75121 aaaaaaacat gacagaaaaa gattatctga tccttaactg gaatcagtca gaactacaag
    75181 aattagcgca aagaacaaaa gcacgcgtac tgcctttctc gacaaaagaa gtgttggaag
    75241 acggtgtgta tgctgacgat tactcaatat actataaaaa agaaaaaata atggaaatat
    75301 ctgaacttgg cgtaccaggt aagcataatg tagaaaatgc actagctgct atttctgtag
    75361 caaaattgta tggtatatca aatgaagcta tacgagaaac gttgcatttt ttccatggcg
    75421 taccacatcg tacgcaatat gtaggagaaa tccaaggcag aaaattttat aatgattcaa
    75481 aagcaaccaa tattttagcg actaaaatgg ctctaagtgg atttgaaacg tctaaggtcg
    75541 tgttattggc tggcggatta gacagaggaa atacatttga tgaattaatt ccttcattaa
    75601 aaggaatcaa agcaatggtc gtatttggcc aaacaaaaga aaagttgatg gatgctggaa
    75661 aaaaagcagg catcgagaca atagtaacag ccgattctgt agaacaagca gttcctttag
    75721 cattagaaaa cagtacagat ggtgatgtag ttttattatc accagcgaat gccagctggg
    75781 atcaataccc aaattttgaa actagaggaa acagattcat ggaagctgtg aaccgattaa
    75841 aatagaaaaa gtagaagcga gcgataaaaa atgaaaattt tggttacagg cggaggaaca
    75901 gggggacata tttatccagc tcttgccttt gtcaattatg taaagactca agaaccgaat
    75961 gctgaattca tgtatgtggg agcaaaacga ggtctagaaa acaaaatcgt ccctgataca
    76021 ggaatgcctt tccatacctt ggagattcaa ggatttaaac gaaaaatttc catgcataac
    76081 atcaagacgg tccgattgtt tcttaagagt atccgtcaag caaaaaaaat cctcagtgat
    76141 tttcaaccag atatcgttat cggtactgga ggatatgttt ccggagcggt agtgtacgct
    76201 gcagccaaaa tgggtattcc aacagttatc catgaacaaa acagtgtacc aggtgttacc
    76261 aataaatttt taagccgata tgtggatcgg atcgctcttt catttaaaga tgcggcttcc
    76321 ttttttcctg aaaacaaagc agttcttgta ggtaatcctc gagcacaaga agtagcagat
    76381 actaaaaaat cagatatttt gaaaacattt gatttggatc cagaaaagaa aacagtcttg
    76441 atttttggcg gtagtcaagg agcacttaaa atcaatcaag cagtatcagt ttttttaagt
    76501 gaatatattc cttcttctta ccaagttttg tatgcttcag gagatagata ttatgaagag
    76561 atcctaaata aaataaatcc tgaaatacaa aatattagtg tccagcctta tatcaaaaat
    76621 atggcagagg tcatggcttc cagtgatctg ctggtggggc gtgctggtgc aacttcgatc
    76681 gcagaattca cagctctggg gttaccggca gtgttggttc ccagcccata tgttacgaat
    76741 gatcatcaga ctaaaaatgc aatgagtctt gttcacgcag gagcagcaaa aatgattgca
    76801 gataatgaac taacaggtga atcactaagt cagacagtca acgaaatcat gggtgatgaa
    76861 gaattgcaaa agcaaatgtg ccgagcttct aaagaacaag gaatccctga tgcttcaaaa
    76921 cgtctttatg acttagtaaa acaaataatc aaaaaataaa ggcggtggtc ttagtgagta
    76981 agaaaaaaga accaaaaaaa ttgtctcatc cgtcttctga tgaaaacaat ttgactcctt
    77041 ggcaaaaggc taaccgaaaa tatctggcag aacaagagat gaaaaaccaa gagccaaaaa
    77101 aagaagacat aacattagat acaccccttt cagattctga agaagaaaaa acggttcaag
    77161 aggaacagca agaagaaagc cagtccgaga aaaaaatcga attttttgaa gagatcaagc
    77221 aaaagaaaaa cggtggacca tataacggtt cttttttgaa ccggctgccc aatttaaaaa
    77281 gtcaacggca caaagtacta tttcgtcggt tagctttgat catcactgta ttgggtatac
    77341 cgctgatttt tttgatctat tatgtttctc cttatagcaa attacaagca gttgcagtat
    77401 caggcaataa gacagtcaac agccaagaaa tcatctctga tacaaaattg tcattaggag
    77461 aaaatgtttg gggacagtat tttcatcgat ctacttatat cgatcgcctt aaaaaagcac
    77521 agccaagaat cgaaacagca aatatacatt tcaaagggat gaatgaattc gatttggatg
    77581 taacagaata taaagagatt gctttgatcg caaaaaacaa tcaatactac ccagtgattg
    77641 aaaatggaac agtattagat gaaaaagtag caaatcctac aaaaaatctc cctatcttgg
    77701 aagagttcaa agactctgca aaaatcaaag aattaacaaa gcaatacaat cagttatcat
    77761 cagaattaca aaaagcaatt tctgaaatca agtacacgcc aagagcaagc aataaaaatc
    77821 tgatccaatt gaatatgaat gacggcaatc aagttattgt aaatatcaat aatcttgcga
    77881 accaaatgaa atattattcg caagtagcca aagatatgga tgaaaaaggt gtgatcgaca
    77941 tggaagttgg tatcttctcg catccttacg gtagcactca agaaacggaa gaatcgggcc
    78001 aatcggaaga aacaactgaa agtgaagaaa atgcggatca aacaaacgaa tcaagtgaag
    78061 aggcagaaac gcaaaaagca caaacacctg gatcgtctag cacaaacgga tcatctgatt
    78121 caaatgattc aagtgaaaca agtcaaatag aagataatcc aagtacaact ccgtcaagtt
    78181 taaatgaaaa ttgataaaac aactttaaaa agtaagaatg tactttctaa aaaccagtaa
    78241 tttatggtag aattggaagc agtgaatata tctattaata agtatatttt aaaacggacg
    78301 ctagttgttc gcttgtatat ggaatagtaa gtaggaggga atcccattca tggcaaaaac
    78361 aggaatgtat gtaggccttg atattggaac gacatctgtt aaagttgtcg tggctgaata
    78421 tatcgacagc caaatgaata ttattggagt aggaaacgca aaatcagaag ggattaaccg
    78481 aggcattatc gttgatatcg ataaaacggt ccaagcgata caacgagcag tacgacaagc
    78541 agaagaaaaa gcaggtatcc agatcaaagg cgtaagtgtc ggtttgccag caaacatgct
    78601 tgaagtagaa aattgccagg gaatgattgc agttaacgga gattcaaagg aaatcaccga
    78661 tgaagatgtc agaaatgtgg cttcagctgc ccttgttcgt tcaatacctc ctgaacgtca
    78721 aatcgtgtca atattgccac aagattttac tgtcgatggg ttcgagggaa tcaaagatcc
    78781 tcgtggaatg atcggcgtaa gattagaaat gtacggtctg ttgtttacag gacctaaaac
    78841 gatcatccat aacatacgta aatgcgtgga aaatgccgga ttaatcgtca acgaaatggt
    78901 gattactcct ttagcgctga ctgaatcaat tctttctgac ggagaaaaag attttggtac
    78961 gatcgtcatt gatatgggtg gcggccaaac aacaacggct gttatgcatg ataagcaatt
    79021 gaagtttacc aatcttgacc aagaaggtgg cgaatttgtc actaaagata tctctattgt
    79081 attaaatacg tcatttaaca atgcagaagc gttgaaaata aactacgggg atgcataccc
    79141 agaacggaca tcaccagatg aagaatttcc agtagatgtg attggtcagt cagaaccagt
    79201 gaaagtcgat gaacgctact tgtcagaaat tatttcagct cgaatggaac agattttcaa
    79261 caaagcaaaa gatgcattgg accaaatcga agcattagag ttgccaggtg gcgtcgtatt
    79321 gacaggcgga gctgctagtc ttcctggtgt tgtggacttg gcacaagaaa tcttcggcgt
    79381 aaatgtaaaa ttgtatgttc caaatcacat ggggctgaga aacccggtat ttacaaatgt
    79441 aataagtatc gtggattatt cagcaaactt gagtgaagtg tatcaattgg caaaaattgc
    79501 tgttacgggt gaaacctcag cagctcgtca aatggtagtt gaacaagaaa caacaaacac
    79561 atatgagagc tatgaagcac ctgaagaaac aatctacgat gagccggaac caaaagagtc
    79621 cggagaaaat gtaaaaaata aaatcaaggg tttctttaca aatatttttg attaattcat
    79681 cctgggagga ataagatatg gaattctcaa ttgataataa tatcaatgac ggcgcagtaa
    79741 tcaaagtcat cggagtcggt ggtggcggtg gaaatgctgt aaaccgaatg attgaagaaa
    79801 acgttaaagg tgttgaattc attacagcca acacagacgt gcaagcatta aaaaattcaa
    79861 aagctgaaac agttatccaa ttaggcccca aatatactcg tggtttaggt gctggttctc
    79921 agccagaagt tggacaaaaa gcggcagaag aaagtgaaca atccttacgc gaagctttag
    79981 acggtgcaga tatgattttc atcactgcag gaatgggtgg aggaaccggt acaggtgctg
    80041 caccaatcgt tgcaggtatc gcgaaagaat tgggcgcatt gactgttggt gtcgtgacac
    80101 gtccattcac ttttgaagga ccgaaacgtg gccgttttgc tgcagaaggt atcgctcgtt
    80161 tgaaagaaaa tgtggataca ttattaatta tctcaaataa ccgtttatta gaagttgtag
    80221 ataaaaagac accgatgctt gaagctttcc gtgaagcaga taatgtttta cgacaaggtg
    80281 tacaaggtat ctctgatttg atcacagcgc caggttacgt taaccttgac tttgctgacg
    80341 tgaaaacagt gatggaaaac caaggtacag cattgatggg gatcggtgtg gctagtggag
    80401 aagatcgcgt agtagaagca acgaaaaaag caatctcctc tccattgctt gaaacatcta
    80461 ttgatggagc tgaacaagta cttcttaaca tcacaggcgg tttggatatg acattgttcg
    80521 aagcacaaga tgcttcagat atcgtagcaa atgcagcaac tggcgatgta aatatcattc
    80581 taggtacatc tatcaatgaa gaaatgggcg atgaaatccg tgtgacggtc atcgcaacag
    80641 gtatcgatga atcaaaaaag gaacgcaagt ctagccgacc agctagacaa gcacaaatgc
    80701 aatcacctgc tcaaaagact gtattagata tggatcaagc taaaccaact tcttcagaag
    80761 aagaaaacag ttttggcgat tgggatatca gacgtgaaca aaatgttcgt cctagagttg
    80821 atgattccaa ttttgacaat atcgaaaaaa aagaatttga tacattcaac cgcgaagaaa
    80881 caaaatctca tggcgatgat gaattaaata cgccaccgtt tttccgtcgt aaaaaataaa
    80941 ggagaggaag agcacatgat tgctgacaac ttgcagaaaa tcaagcaaga aattcaggat
    81001 tcgtgtgctc ttgttcatcg tgatccgaaa gaagttacat tagtagctgt aacaaaatct
    81061 gttgaatccg ctcaagctga agaattggtt agagctggcg ttatgaattg tgcagaaaac
    81121 cgagtagata aattattaga aaagaaacaa gatttatttg attttcctga aataaagtgg
    81181 catttaattg gtaatttaca gcgcagaaag gtaaaattga ttgtaaatga aattgattac
    81241 tttcatgcgt tggacagttt aagattagca gaagaaatcc agaagcggtt agagaaagaa
    81301 ttatcctgtt ttattgaagt aaatgtttct ggtgaggaat caaaacatgg aatttcacca
    81361 gatgagcttt taccgtttgt ggaatcttta gctgcctgca caaaaatcaa ggttgttggg
    81421 ttgatgacta tggcgcctaa agaagcttct gatgagcaaa tccataaagt gtttggtacg
    81481 ctgaaagaat tgcgtgatct tgttcaagca aaaggttatg cccatgcacc atgtagtgaa
    81541 ttaagtatgg ggatgagtca agactttcaa attgcgattg aagaaggtgc aacatttatc
    81601 cgtgtaggaa cagcgctatt caaagacgaa taaaggagga acaaaaatgc cgcttttaaa
    81661 cagaggagca attgctaatt ttttcggatt agctgatgag gaagagtacg aataccaaga
    81721 ttatcctgaa acaacaaaaa aggctgtaca gccaactaaa aaaaatacaa cacagaatca
    81781 atcaactcat tctgtatctg ctgcgaatac tcatgaaaaa caaaaaacgg cgacttcacg
    81841 tcctgtaacg catgaaagta ttcagccggc tgaaaagaaa aaagtaattg aactacatca
    81901 atcacctaca aaaactgtag cgaaagaaag aacagcttca agaacctcta cacaacctaa
    81961 agcaaatctt gctggaaaaa tcactgtaat ggaaccaaga gcttattctg aagctatggc
    82021 gattgctaaa cgaatcattg ctggagaagc aaccttagtg aattttcatt tagtagaaga
    82081 aaaacaagcg agaagaatcg ttgattttct aacaggaaca gtttatgcac tagatggaga
    82141 tatcaaacgt gttggagatg agatttttct ttgtacaccg actgggatgg agattgacag
    82201 ttcaaccgct caatcttttg cagatgcgaa tttgtttgat ctgtaagagg aggaactacg
    82261 atttatacta ttgcactatt acttcattta cttcaacggg cagcctattt ctatagcatt
    82321 atcttgatgg tatatgcatt gctctcttgg tttccaggcg ggtatcaatc agggctagga
    82381 cgattattag ctaagatctg cgaaccgtat atcagtttgt tcgatcgtct gcctttgagg
    82441 attggaccct tggatttcac aattttaggg gcaatcctga tcttgaactt agccgtacaa
    82501 ggtttagcac agatcgttat atggataact gtccatttag tttattgatt gaagcaacaa
    82561 aatctttatt ctcagaatag ataggagggg aaaattttgg atgcaaatgt ataccagcac
    82621 tttcgggcag atgaacgtac atttatcgat tcggtaggcg attggattga acaggttcaa
    82681 ggacaatatg caccctatct gaccgaattt cttgacccaa gacaatccta tattttagaa
    82741 acactgattc gacaaagcag tgatttgcgt ttcatgtttt atggcggata tgaacaggca
    82801 gaaagaaaaa gatgtttgat tttccccgat tattacgaac ctgtagagga agattttgaa
    82861 acagcattgt atgaaatcca ttatcctagc aaatttgcca gcctaagtca tgggaaaatc
    82921 cttggaactt tagtgggaac ggggatcaaa cgctcttact ttggcgatat catctcggac
    82981 ggtgatcgct ggcaagtatt tattgcaaat gaggtggatc attttgttgc tgcacaagta
    83041 acaaagattg gaaaggttgc tgtacgcttg gaaccaatca aatacacaga aatgattatg
    83101 ccgaaagata gctggacaca agaaagagga acagttagtt ccatgcgact agacagtgtg
    83161 atctctgaga tttataatat ttccagacag cggtccaagc agctgatcga agctggtaaa
    83221 gtaaaagtga actggacaga aaatacaaga cctgattttg tacttgaact attagatatt
    83281 atttcagtca gaggttatgg cagagtccag atccaagaac ttgaggggaa gacgaaaaaa
    83341 gaaaagtaca gggtactgtt cggtgtatta cgtaaataaa ataaaaaggt ggtttactca
    83401 tggctttaac tccattagat atccaaaata agacattccc gacaaaaatg cggggatata
    83461 atcaagacga agtagatgat ttcttagatt tagttgttcg tgactatgag gaattgactc
    83521 aaagaaatcg agaattagaa aaagcagtca aacattctga agaaaaactt gagtacttca
    83581 atgaattaaa agatgctttg aaccaatcaa tcatcgtagc tcaagatacg gctgataaag
    83641 taaaaacaag tgcaagcaag gaatccgaag tgatcgttac ttctgcacaa aacaaagcag
    83701 atgagttagt tgcgaacgct gaaaaacgtg cacatcaatt aacaactgat gcagaagaaa
    83761 aagcaagaaa aatcttgaca gatgcaacag aaaaagcaag acagcttgcg acagaaactg
    83821 aagatcttaa gaaaaaaaca cgagtattcc atcaacgtat cagcttgatg cttgagtctc
    83881 aattagaaca agtgaaaagt ccggaatggg atgaaatttt acaaccattc tccagctacg
    83941 tgactgacag ccatgaagtg atcaaagaag ttttagcaaa acagcttgac aatgaaaatg
    84001 atactgatgt aaactcagac acaggagtca tagaagcacc tatgacggca tttgatacac
    84061 aggctattga tatctcgatc gtgcctggtg aaaatgaaaa cgagtagaac agaaaagcac
    84121 tttgttattc ttaaagcgag ccgatgagag tggaagttcg gtaagacact gcatggtgaa
    84181 agatcctcta cttgagacct gcttttccga aattagagta agaagcagcg gctgacctcg
    84241 ttattggtca cagagagttt gcactagata gatgcaacta aatttgggtg gtaccacggc
    84301 atgttcgtcc cttgcggctg aacatgcttt tttgtgtccg gaaaacaaat aaaggagtgt
    84361 atggttgaat gaaaatgaaa gaaaccttgc aattaggaaa aacaaaattc cctatgcgtg
    84421 gtaatttgcc aaaccgagaa ggcgagtggc aaaaagaatg ggaagaaaat cgaatctacc
    84481 aaaaaagaca agagttgaac gaaggaaaac ctagctttgt tcttcatgat ggacctccat
    84541 atgccaatgg taatatccat ttaggacatt cattgaataa aatcagtaaa gacattatca
    84601 ttcgttcaaa atcaatgtct ggctatcgtg caccatatgt tccaggatgg gatacacatg
    84661 gattgccgat tgaacaagta ttggctaata aaggcgtgaa acgtaaagaa atgtccatgg
    84721 cagaatactt ggaaaaatgt cgtgaatatg cactttccca agtagataag caacgagcag
    84781 atttcaaacg cctaggagtt gcaggagatt gggaacatcc ttatgttaca ttagatcctt
    84841 cttatgaagc tgcacaagta cgcgtatttg gtaaaatggc tgaaaaaggc tatatttaca
    84901 aaggattgaa accaatttac tggtctccgt caagtgagtc ttctttagcg gaagctgaaa
    84961 ttgaatataa agatgtaaaa tctccatcta tttttgtagc tttcaatgtc aaagacggca
    85021 aagatattct tgatgaagat acagcttttg tcatttggac gacaacacca tggacattgc
    85081 cagcaaacca aggtattgct gtaaatccaa aatttacgta tgtattggtg gaagccgatg
    85141 gccgtaagtt tgtcgtagcg aaagatttgc tagaaacagt ccaatcagaa atcggttggg
    85201 aaaatgtaac agttctaaaa gaatttgctg gacaagaaat ggaatatatg actgcaagtc
    85261 atccattcta tgaacgtgaa tcacttgtga ttttaggcga tcatgttact ttagacgctg
    85321 gtactggatt agtccatact gctccaggac atggggaaga tgactatatc gctggtagca
    85381 aatataaact atctgtagtg tcacctgtag actcaaaagg attgtttaca gaagaagcac
    85441 ctggatttga aggtatcttt tatgacaaag caaatcctat gatcactgat ctattaaaag
    85501 aaaaaggagc tttgctgaag ctagatttct ttactcatag ttatcctcac gattggcgga
    85561 cgaaaaagcc tgtgatctat cgggcaactc cacaatggtt tgcatctatc gataaattcc
    85621 gtcagaatat tttggatgaa gtagaaaaag tagagtgggt tatcccatgg ggtaaaacac
    85681 gtttgtataa tatgatccgt gaccgcggcg attgggtaat ttcccgtcag cgtgcatggg
    85741 gagtaccact tccgattttc tatgctgaaa atggagaagc aatcatcaca ccagaaacaa
    85801 tcgaacatgt ggcaaaatta tttgctgaac atggatcaat tatctggttt gaaaaagaag
    85861 cgaaagaact tcttcctgaa ggatttaccc acccaggttc tcctaatggc gaatttacaa
    85921 aagaaaaaga tatcatggat gtctggtttg attctggttc ttctcatgaa gctgttttac
    85981 gtcagcgacc agaattatca ttcccagcag atatgtattt agaaggatca gaccaatatc
    86041 gtggctggtt caactcaagt atcacgacta gtgtggctat caatggtgta gcaccataca
    86101 aatcagttat ttctcaagga tttgctttag atggtgaagg ccgtaagatg agtaaatctt
    86161 taggaaacat cattgcacca gaaaaagtga tcaaacaatt tggtgcagat atccttcgct
    86221 tatgggtatc tagtgtagat tatgaagcag atgttcgtgt ttctatggat atcttaagtc
    86281 aagtggcaga agtatatcgt aaaatcagaa atacgatgcg tttcttatta gcgaatacag
    86341 aagatttcga tcctaaaaaa gatactgttt cgtacaatga tttacgttct gtagacaaat
    86401 atatgaccgt tcgattgaac caagtaatca aagaaatccg agaagaaggg tacgacaaat
    86461 ataacttcat gcatatttac cgtacagtta tgaacttctt gacggttgat ctatcgtcat
    86521 tctacctgga ttttgctaaa gatgtagttt atattgaagc agaagatgat tatcaacgtc
    86581 gttgcatgca aactgttttc tatcaaacag ctgtagcttt gacaaaatta ttgactccta
    86641 tcattccgca tacagcagaa gaaatctggt cattcctgaa agaagaagag gagtacgttc
    86701 agttaagcga gtttcctggt tatgaagagt ttgcaaacca agaagaattg atggatacct
    86761 ggactgcttt catggacttc cgtgataatg tcttaaaagc attggaagaa gcaagaaaca
    86821 gcaaattgat tggtaaatct ttggaagcaa aactaactgt ttatccaaaa caacaagttc
    86881 gtgaaatgtt aaaagctttg gatgcagata ttgcacaatt attgatcgtt tcaccagact
    86941 actttgaagt tatggcagca gatgaacaag tgccagataa cgctatggta tttgatgatg
    87001 ttgctattac agtggaaaaa gcagacgggg aaacttgcga tcgctgtcgc caagttcgta
    87061 aagatgtagg agtagatgaa aaacttcctc atctatgcgg tcgttgtgca aaaatcgttg
    87121 aagacttcta tcctgaagct gtagctgaag gattcgaaga aaagtaaaag ggaaaagggg
    87181 gtgtgacatg ttctttgtca cacccccttt tgttatcaat cgtttaattt tccacgttca
    87241 cgataccagt tatctggttg ccagatccat tcgaatccgt ctttttcaag tagttcgaaa
    87301 gctgctttag gtcccattga tccagctttg tagttgggaa aatccggttg tgtttggtcc
    87361 caagcttttc gaatgacatc cacgatatgc caagattgag cgacttcttc ccaatgagag
    87421 aagttcgttc catcaccatt caacgcatct aatagtaatt tttcgtaagc ttctgggcta
    87481 ttttcaacca tttctgcact atttctaaat tccaatttga ttggttccgt ttcaaatcct
    87541 tgccctactt ctttcccatt caaggataaa gaaaatcctt cagttggctg aatatagatc
    87601 gttagaacat taggtggaag tgtcgtatcg tcacatggtt catcgacaga tgttttgaat
    87661 acattaacag gaacttgttt gaatacgatg ttgatccgag tccctttttc agtcaaacgt
    87721 ttgcctgtac gtacatagaa aggaacacct gaccaacgga agttatcgat agtgaatttg
    87781 cctgcgacga atgtttcagt ctctgattga gcatccactt tgtcttcttc gcgatagccc
    87841 ttgaattgta caccatccaa ttcaccttga gcatattgcc cgcgaacgaa gttttctaac
    87901 acttcttctt cgttataaat gcggatagct ttcaatgctt tgatcttttc tgtacggatt
    87961 tctttttcgg aaaatgctac tggcggttcc attgcaagta atgctacaac ctgtaaaata
    88021 tgattttgca ccatgtcttt aagtgcgcca ctatgatcgt agtagccccc gcgatcttct
    88081 acaccgaggt tttcgccaaa agtaatttgg atattgtcga tatagcgatt gttccattga
    88141 gcctcaaaaa tattattggc aaaacggata gctgagatgt tttggatcat ttcttttcct
    88201 aaataatgat caatcctgaa aatatcttgt tctgggaata cttctctgat ttgctggttc
    88261 aattcaaaag cagactggta atcagaaccg aacggttttt ctatgatcaa acgattgaag
    88321 ccattttttg tcataatatt ttgtgatttc aaatgagaaa cgatcgttcc aaaaaattga
    88381 ggtgccatag ccaaataata gacacggtta ccgtttaatt gatagtgtcc atctaatcgg
    88441 tcagacaatt cttttaatgt ctgataatgc gttgaatcat ttacatcatg ggattgatag
    88501 taaaaatggc ttgcaaaggt agttgcttct tcatctgaag gattcaatgc acggattgtt
    88561 tctttgacaa cttcacggta gtgctcatcg ctccagggac ggcgagcggt tccgattaca
    88621 gcaaaatgct cgcctaaatt tccttttctg taaaggcgga acaaagacgg gtacaattta
    88681 cgctgggcta ggtcacctgt accgccgaat atcgtaaaca atacattttt ttcattagac
    88741 atgtggatac actcctttat attttatcga caaaaatcgt gctggccgcc ttaaagctga
    88801 cggggatttt ctttctatca ttttggatag tgatcggtcc ttcataagct gcgatgtcaa
    88861 cgatctcata ttcttcgttg atattcaggt caatagtgac taagtaatcc aacaattctt
    88921 tttcatcaag cactcgtgcg atacgcactt ttgtaccgac aggataggac tccaatgttt
    88981 gtcggcgttc ttcatggaca acttcctctt ttttaggaat cgatcctcca tgaggacaat
    89041 gttcaggatg attcaaatag ttttctaaat gatctgcaag atgttccgaa gtcacatgtt
    89101 ccaatacttc agcatcatca tggacttcat tccagctata gtctaaatgt tcaactaaaa
    89161 aaacttccca taagcgatgt ttgcgaatca gactgctagc tttttgcagt ccgcttgcag
    89221 tcaattgtac accttgataa ggggaatgtt ctacaagctc ttctttcaca agtttagaaa
    89281 tcatttcact gacagatgca gctgagacat ctagactaga tacgatttgt ttattgttga
    89341 ctttcgttgt atctccacct agttctaaaa tgattttcaa ataatcttct cgatttggtg
    89401 tcatattcga tcatcctttc atggtaaaaa accatcatta gtaagtgtaa caaaaaaaac
    89461 gttaattgac tatttttcta aataaaaaac aaaggatttt tagacgtttt tacaacaaaa
    89521 aacgaaagat gatagagaag actcattctt tcgtttataa aggatatata tatacattgg
    89581 gagggatcta tttttcagat aacaacttat atgaagtgtc tgcagattcg tcataaagag
    89641 tttgttcggg ataagaaaag ataagataaa aaccatactg atcacgtacg actaaacgat
    89701 attcatctat ttctaccaca gatgtgccaa gagactgacc gttgatcaaa aacgtgtaat
    89761 cagatgtaat ctgtaattgt tgtttttgct ggctatcact aaagaaccat gtaccttgat
    89821 actttgagag tccttctgac ttttccgctt gttttttctt tttcaagtaa ataaaacttg
    89881 agagaacggt aatgagtgaa ccaagaaaat agatcatttt ttttatcacc aaatcactcc
    89941 tttatgagaa aataatcgca tagacatatt ctttcttctt tatttcattg taccgaataa
    90001 aagctaaaat ttctctaaga ctttcgactg gtttttttac tgtttttcta caaattatga
    90061 taggatacat agtaggaaga aaggtgacat aatttgaagt atcagcaagt ggaaatagct
    90121 ttttttattg accgcccgaa tcggtttata gcacattgta tgaaaaaaga cggagaaata
    90181 attattgcgc atgtcaaaaa tacaggtaga ggaaaagagg tcctgatacc aggcgctgaa
    90241 gtggctattg tatttgctcc tggacctaaa cggaaaaccg cctatgatct gattgctgta
    90301 aaaaagaagg gtgactggtt taatatcgat agtcaattgc ctaatcggct ggcaattgat
    90361 ggtattttag acggcacgat ccttttacca ggattaactg gtccatttac ggtatacaag
    90421 cgtgaagtaa cttatcttca ctccaaattt gatatttatt tagagacggt ggtcggacaa
    90481 aaagcatttg ttgaagtcaa aggtatgaca cttgaaaata aagcgattgg tgcttttcct
    90541 gatgcgccca cactcagagg attgaaacat ataggagaat taacgtatgc cgcacaagat
    90601 ggttatgccg cttatgtctt gtttattgct cagttcgaac atcttcatct ggcaacgatc
    90661 catgaagaaa tgcaaccagc actagctgat atggttcgac atgcgcagca aagcggagtt
    90721 caaatcctcg cctataattg tcaagtatcg gcagatgagg taactgtcaa gcaggccatt
    90781 ccatttgatg tagatatccc ttttgaagat cctatacaat agaaagaagg taaaataatg
    90841 atcaacttag gtattatcgg aacaaattgg attactcatc aatttgtcca agcagcttta
    90901 agcacaaaaa aatatgaatt gacagcggta tattctagac atttggaaaa agcacaggaa
    90961 tttggttctc attatgaagg ggatatcgaa ttcgcaacag atttgaagac ttttttcaat
    91021 attactcata tggacaccat ttatattgct tctccgaata gtcttcactt tagtcaagct
    91081 aagcaagcaa ttttggcagg aaaaaatgtg atagttgaaa agccagcttt ttcgacgcca
    91141 aaagaaatgg atgaaatcat ccatttggca aatgaacaca atgtgtattt ttttgaagca
    91201 gcaagaaata tccatgaatc aggttttaaa aagattgccg attacttacc attaaaagac
    91261 gagataatcg gtgcaaattt cagctatatg aaatattctt cgcgctatga ccaagtatta
    91321 gatggagaag aaccgaatat cttctcgact catttctcag gtggtgcatt gatggattta
    91381 ggtgtttacc ttgtatatgc agctgttgct tggtttggaa tgccaaaaga agtccattat
    91441 ttcccaagaa agatttctac tggtgtagat ggtcttggat gggggatctt acgttatgag
    91501 aattttgatg tatcgatcca gccaggtaaa atcggtgatt cttatttacc gtcagagatt
    91561 tattttgact caggaacgct agtgatgaat ggagtcaatg caattgaaca agcacaattt
    91621 catgatcgcg cgcataaaga aatcattgat ttggcggtaa cagctgcaga aaatccaatg
    91681 gcagatgaag cagaggattt tgccaatgtc atggctcatc cgaaagatcc agcttgggga
    91741 ttgttgtatg aagagtggat cgagctagct agaaatgtga accaagtgat ttatgattta
    91801 agaaaaaatg gcggcatcaa attcgatgca gataatgagg aagattttta atgaacgaat
    91861 ttgtacaagt atttcccgaa aattggcaga aaaaatatga ggataaaggg tttatctcac
    91921 cttctcttat tcaacgagca gtttttcagc cactaagtaa taaacaaagt atagtagcgg
    91981 tttctcctac aggttcaggt aaaacactgg cttatctttg gcctctacta ctaaacgtgg
    92041 aaccaggtga agcaagtgcg ttggtcattt ttgcttcttc tcaagaacta gctattcaag
    92101 tagcagatgt tgcacgtgaa tggggaaaag acaaagagtt gaaagtccaa tctttggtgg
    92161 gcggagccaa cgtcaaacgt cagattgagg gtttgaagga aaaaccagaa atcttgattg
    92221 gtacaccagg aagaatattg gaattgatga aagcaaaaaa aataaaagca catcaagtga
    92281 aaacaatggt atttgatgaa gcagaccagc tatttgacgc tgggaacagc caaatcattg
    92341 atcaaattct tcatcaagct ccaacagagt atcagctagc ttttttttca gcgacagcag
    92401 atcgttccct tgaaagcatt gaaaaaatca ctgggaaaac gtttgagaca attgacgtga
    92461 cagcagagga cgattctcga aaaggactga gacattactt tttacgtgta ccgatacgaa
    92521 agaaagatga ctacttgaga cgtctcacgc atatagaaga ttttcatggt ctgattttct
    92581 ttaatcaact gactgaatta ggcgtaatgg aagaaaaatt gctgtataga ggtgtaccgg
    92641 taggaagtct ggcctctgat cagaacaagc tactccgaaa agcagcgcta gaacaattca
    92701 aaaagaaaat ggtcagagca cttttgacca cagatgtggc ggctagaggt ttagatatta
    92761 caggtcttcc ttatgtagtg aatgcagaag ttccgttaag tgaagaagct tatttgcatc
    92821 gttcgggaag aactggtcga atgggtaatg aaggaatagt aattacactc gtacaagata
    92881 ataatctaag agatttaaaa cggcttttac gtccatcaaa tataacactt gaagagattt
    92941 ttttatacgg aggtaagctg caaactgagc agccaaccaa agaagatgca gctcctgcag
    93001 taaataaaaa gtatgcgaaa actttttctg gaaaggagca gaacccagaa tataacaaag
    93061 aaaaaataag aaaaaaagtt aaaaataaat caaaaaaaga gagaaataaa ggagcaaggc
    93121 gcaaataagc gcctttttat gctatgatta tgaaaacggg aacaaaattg gttgccagag
    93181 gaggggttat gtgaaagaac gtcaagaaga gatcttgcag ctattgacag aaaaccaaca
    93241 aggattgaca gcaagtgatg tagctgagcg cttaactatc gatcgaagca atgcaagccg
    93301 atatttaagt gaactataca aggcccatca tattgtaaaa accgctggcc gaccagtcgt
    93361 ttactctttg ccaacggaaa agtcaaaatc tgatgaagtg catgtcgact cttctacgca
    93421 agtgactttt gaaacactcg ttggagaaaa tgattcttta aaagtcagca tacagcaggc
    93481 aaaagctgcg attctttatc caccgcgggg gttgcatacg attatttttg gagaaactgg
    93541 tacagggaag tcgatgtttg ctgaatgtat gtatcatttt gcaatcgatt cagagatgct
    93601 ttctgcagat gcgccatttg tttctttcaa ttgtgcggat tatgctcaaa atccgcaact
    93661 gctctttgga cacatctttg ggatcaaaaa aggggcttac acaggagcag ctcaagatag
    93721 tcccggtcta atagccaaag cagacggcgg tatattgttt ttagatgaga tccaccgttt
    93781 accgccggaa gggcaggaaa tgctgtttac tttcattgat aaaggtattt accgtccgct
    93841 tggagaaagt gcacaagtac atgaagcatc tgttcagatc attggggcga cgactgaatc
    93901 ttcagaaagt ttcttatcta cgttcaatcg acggattcct atggcaatca ccttgccgaa
    93961 tcttgcttca cgttcactgg atgagcgtta tgaaatcatc tctcttttta tcaaacaaga
    94021 agcgaatcgt ttgaatcaac gtatcgatat cgaaaaagaa gcgattcttg ccttcatgct
    94081 ttatgatgcg gaaggaaata tcggtcaagt gaaacgagac ttgaaactag tctgtgcaaa
    94141 atctttttta cattaccgta ctcatcatga gaaaaagcta atcattcgaa aagaagaact
    94201 ttcccttcaa gtacaaaaag gattactgaa aatcaaggaa ataccggaac gtctcgaccg
    94261 attcatggac agcaaaagtc agtatttgac atttgaacca ggatttgctg atgttgtctg
    94321 gtcacaagat ccagaacgaa acatgcaggt ctataacgat attgaggaaa aagtcacttc
    94381 tctttccgaa accggtgtgg agaacattga tctagagacc ctcatttcaa aagatgtgga
    94441 tgcttatttc cagacatatg tcaaagaatt gactaaaagt tcgattccca aagatctttt
    94501 gccagatgaa atctggcggt taaccaatca attgtatgat gtggctgaaa aacggttaga
    94561 ccgtatttat aatgaaaaag ctcgttttgc ttttgctttg catctgcaaa gtacattgga
    94621 tcgtgtgaga gaaggtcata tgatcgtcca cccagatttg aataacgtac ggaaaaattt
    94681 taagagggaa ttccaggtag caatcgatct ttcaacgatc attgaagaag aagaacaagt
    94741 tgagattcct tttgatgaaa tcggctttat tagtatgttt ctttctatca atctaggaga
    94801 aagtgagcct gttaaagaaa acaaagtaga tgttctcata ttgatgcacg gcagacagac
    94861 tgcttcaagc atgttggaga tggcacaaga actattagga acaaaagtag ggaaagcttt
    94921 taatatgcca ctggttatgg aagttcaaac aatgtatgaa gaagtgttac aatatgtcaa
    94981 aaactatcaa gagcatttga cgaatggcct actcttgtta acagatatgg gatcattaaa
    95041 caccttcagt gatttgattt atgaagagac tggcattcgg acgaaagcta tcacgatgac
    95101 aagtaccatg attgtgatag aagcgatacg gatggcaagt gttggaagaa gcttagaaga
    95161 tatttatcaa aatatccagc tttcctttga atcgatcgta cgtgatcagt tcaaagcgga
    95221 aaagtctgaa actttacagc gtaaaaaagc cgtaatcgtg acatgcttta ctggtgaagg
    95281 agtagctgcc aaattatatc agcgtattat gccagtgatt aatcaaaata aggtagaact
    95341 gatccagatg cagtttttag aacgtgaatc attcaaaaaa cacatcgatg ccttattgga
    95401 agaatatgag atcaaagcaa ttgcgggaac agttgatgtt gattatcaaa atatcccatt
    95461 tttttcagcc tatgacgttt ttgatgatga aaaactaaat attttaaagc gtatagctga
    95521 tgatgagatc ccgacagcta aaattgtcga ttcacttgct ggatcattaa gcgatgtctt
    95581 ttctattgat gaactgatcc gttcattgca aaagacagtt cagcagatcc agaccgattt
    95641 gtcgattatc gtggaacctg gagtagatac tggaatcgtt atccatttag ccttcatgtt
    95701 agacggattg atcaaacaag aaagccatcg gcaatttgag aaactgtcta ttttctcaaa
    95761 aatttatcgt ttggaaatgg atatcgttcg ggcaaacttg atggcactag aacgaaaata
    95821 tcaggtaaaa attccagaag atgatatcgc atttcttaca caaatgtttc ttgaaaataa
    95881 aataaaaaaa tgaacacact aatcatactg tttacacact aaacatctgt gtgcgcacta
    95941 taaaaaagag ctaaaaagca tgaaaacgct atttcagctc ttttttttat tggcatgctt
    96001 cttgcgttat ataaaagtgt aaaaaaataa aaggagtgtt aaaaatggca aaaaaaacaa
    96061 tcatgttagt ttgttcagca gggatgagta cgagtttatt ggtcacaaaa atgcaaaaag
    96121 cagcagaaga aaaagggatg gaagccgata tttttgcggt ctctgcctca gatgcagata
    96181 ataatttaga atctaagaat gtcgatgtat tattattagg tcctcaagta cgtttcatga
    96241 aagctcaatt tgaacaaaaa cttgcgccta aaggtattcc attagatgtc atcaatatgc
    96301 aagactatgg catgatgaac ggagaaaaag ttcttgcaca agcagaaaat ctaatgaaat
    96361 aaatcaagta tgcagttggg ggtataacaa cgtggaagat caacaaaatc tagaagccat
    96421 catggggtta atcatgtttg gcggtaatgc aaagagcgat gcaatggaag cgattgcggc
    96481 tgctaaaatc ggtgattttg agttagcgga taggaaaatc gctgatgcag aagaatcact
    96541 tatacaagcg catcatgcac aaacagacat gttgacacaa gaagcacaag gaaatcatat
    96601 gcaagtgaca ttattgactg ttcacagtca agatcactta atgacatcta ttgcgtttac
    96661 tgatttagca aaagaaatca tcgaacttta tcgtcgcgta tacagcgaat aaatacaact
    96721 tcttttttga acggaagaag aaaaattttt gaacggattt tttattttgt tataaggaga
    96781 actctcttta tataaaaaac ttataggagg aagtaaaaaa tgaacggact atcaagttgg
    96841 atggagaaat acatcctgcc ggttgccgca aaaatcggag cgcaaaaaca tttagttgct
    96901 ttgcgtgatg cttttatcgg cacactgccc gcaacaatgg cgggatcagt cgctgtaatg
    96961 atcaatgcaa ttattcgtga tttaccgcaa cagtttaaca gtacctatgc tgacagttta
    97021 gcagcagaca aaggaatctt tgccgtattg aatgtcatca tcggaatcaa tggttttgtc
    97081 tggaacggta cactagcaat cgccggattg atctttgcct tttcttgggg gtataatctt
    97141 gcccgagctt atggagtaaa tgatctagct ggggggatcg ttggtttagc taccttgatt
    97201 caaggtattt cctttggtcc agcattatca gcagcattag atgtgaatgt tccttcaaat
    97261 gtcgcagatg caatcaatag ttctgagatt ggtgcaacga ttgcagatgg cacactgtca
    97321 gttggattat ggggttggct aaaactagat catttgaatg gaaacgctta ttttacagtt
    97381 atgatcatgg gagctatttc agtcattatt ttttgtaaat tgatgcttgc caacattacg
    97441 atcaaaatgc ctgattcagt accaccagca gtatctaaag catttgcagc cattttacca
    97501 gcgacgatcg ctttatacgt cattgctatt atcaattata tcgtaggtcg tgtagctgat
    97561 gggcaactga tcattgattt ggtgcaaaaa tatattgcag agccattcct aggattatct
    97621 caagggttgg gagcagtatt gatcgtcaca gtatttgttc aagtattctg gttctttggt
    97681 attcatggac caaatgtatt ggcaccagtg ctagagggaa tctggggaca agcacaacta
    97741 atcaatattg atattttcca aaaaggttat aacggaaaaa ctggtacacc agccgtgtta
    97801 gatgcaatcg aagatggaaa agcttatatg tgggtccgcg gatcgttcga tgcttttgca
    97861 tggtttggcg gatcaggcgg aacgatcgtc ttactgatag ctatcttgat tttctctaaa
    97921 cgtgcagatt atctaacagt agctaaatta tccattgggc ctggtatctt caatatcaac
    97981 gaaccagtga tgtttggtct gccaatcgta ttgaatgcaa tcatgtttat cccattcttg
    98041 attgctccag tagtagcaac agcaattgga tggacagcta cttatcttgg cctagtcgct
    98101 ccggtatcgc agcaagtcac atgggtagta ccgccgttct tattgtcatt cttagcaaca
    98161 ggagcggatt ggcgggcacc gatcgtaaca ttagtatgta tggtcgtgac tttcttgatt
    98221 tgggcaccat ttgtcatctc tgcaaacaaa atggaccctg ccgcaggcga acaacaataa
    98281 gatgaacgtg aggagcggga cgctttaagt ttcgttcctc ataatgaatt ttcgaggagg
    98341 aactgaaatg attacaattg ccagtgcaat gcaacaagat gaaatcaaag aattgttaga
    98401 aggatacaat gaagaaggaa tgacttttac atttaaagaa aaacaaggaa tcaagctgtt
    98461 ctttgaaaca agtgcagaag atccggaagt tgcggctaaa aaagcaaagg aactaatcaa
    98521 agcacagcct tggggaacag tcctttattt tcaatcaaca gctgtttaga aaatggttta
    98581 aaaagatagg atggttgaga tgataaaaaa cggtttattt ttatgtagta ttggttcttt
    98641 gatcgtgttg tatagtttga atccacattc gcaaaggtat gatttgtata gtatggtcac
    98701 aggtcttttt ctaacaggat taggaatcta tttcttcatg aaggggaaaa aaagagaaga
    98761 agcaaaggag aaaaataact aatggaaatc acgaaaaaat taaatattcc agcatcgttt
    98821 ttttacgata aagttatgga ctctgtcctt ttcgatgtac gtaaagcgac cggcaaatca
    98881 gtaactagaa aacagctgaa aaatttggag tacgtcaaac aattctcgca aaataaccgg
    98941 gctcgcataa aaatcgaaga agtcattgaa aaccagtctt ataagtttcg tacatcgaca
    99001 acgaagaatg aattcgtcgt tcattatcaa atcaaaccat tggacgattc gacctgtgag
    99061 gttcattaca cagaaaaaat ggagtcttat ggcatgcttc aaaagttgaa tgatatgctt
    99121 ttaggaacaa tcttgatgta ttttaaacgg agacgtttca aaaaaatgct agatatgatc
    99181 gaagcaacgt attaaatcaa tgaaacatgg tactatttag ttattgaaaa atgaagtaag
    99241 ctagaaggaa tttttcatag gaagatagat ctttcctgcg aatcctcact agcttttttg
    99301 taggaagggg cagcaaaatg acaaacaaag taagagagct aggtgtatct tcgattgagg
    99361 aaatgtttga tttagccact tatgcattta atggggcaga tacacctgaa aggcgagaac
    99421 gttttcgtac acttgcggag aattcttgga attacggatt ttttgatgag gaggatcggc
    99481 taacaagtca agtcatggcg accccttttc cagtgaattt ttacggacaa gagtacctaa
    99541 tggccggcat tggctatgtt gcttcttatc cagaagcaag gggacaaggc gggatcaatc
    99601 agatcatgga aaagatcctt gaagattgcc gtgatcgtaa agtaagctta tcttatctgg
    99661 cgccattctc ctatcccttc tatcggcgct atggctatga acaatcattt gataaaatca
    99721 gttatcaact aaacagtcgt gatgtgccgt ttatcaaaaa aaaatctgga aaaataaagc
    99781 gtatggattt tgaagatgca aaagctgcaa tcagaagaat ttatgaacag atgccggaaa
    99841 accaacgagc tggtctgaag cgcgaagact ggtggtatac gtataaattc aagcaaaaag
    99901 gcaattacca gtttgctttg cattttgatg ataatgagga agcaacggga tatatcgttt
    99961 atcagcttgc aacgtcttcc tttattattg cggagtgggg atttttggat catgatgctt
   100021 tttgcagcct tgttcgcttc gtaagttctc acaatggtgc ttttgaaaca tttgaatatt
   100081 catgcggcta cggcggtaat gatcaaaatt atctattaga aaatccattt gcttctgtaa
   100141 tgatcacacc ttatatgatg gcgaggatcg tagatatttc tttgtttttg gaaaattatc
   100201 catttaaaaa gagacaagct gcatttgccc tacaaataga agaagatcat tatgcgaaat
   100261 ggaatgaagg aatcgtggaa gtaacaatcg aaaatggaaa aaatcatctc tcaaaagtag
   100321 aacaaacaaa actcccaatc gttcgaggaa gtatccaaca tatcacgcag ctattattag
   100381 ggtatcgtaa agtggaagag ttggtattcc aagaaagaat acaggtgaca aaagaatatg
   100441 aagaaacgtt aagctcaatc ttgccacagc aacagccaat tcttaatgac tatttttgat
   100501 aaaaaacggg tgatgacaat atttttgtca ccaccctttt gtttttcatt tggcatttga
   100561 aggaaaatgt taaagtaaat cctctttttt cgtaaatttg aattttttgt tttcttttgt
   100621 tttagggttt tcttttttgt ggtcttggat tgcttctttc gtaatctttt cccgattttt
   100681 tttagatgaa tctaagcgtt ctgcgtatac tttttctttt ggcttatcat tcattggaaa
   100741 ggacctccct acattatttc attctacctc tagtttatca tctgttaagg actagtggaa
   100801 acgttttgcc taacttataa gctgattttt ttagcagata agcttgcaag taaaaaacaa
   100861 acaagctaca attgatttat tgaaattgtt cctgtagctc agtaggatag agcaatcgcc
   100921 tcctaagcga taggtcgctg gttcaattcc agtcaggaac gtataaataa cccctgtatc
   100981 ctttgtggta caggggtttg ttttgtattt gtgccaaaaa tgtgccaaac tagttatcat
   101041 ttttatataa actatcgaat acagcattga ttttttcttt atcctgatct tcaagctctc
   101101 gaacaatatg aagataagta gacattgtag tttctaaact gctatgtcct aaccgttttg
   101161 atacgctaag tatatttact ccttgataaa gtaaaataga tgcatgtgta tgccttagtc
   101221 catgcaaagt aaattgcttt tcaatcccta attcttttaa ttttttgcgc aaatatttgc
   101281 tgacggcatt tgatgaaact aatccatttt ttaagttgaa aaaaacaaaa ttatgagggt
   101341 ttttaacttc aaaattctcg aataattctt tttgattgat tttaaacttt tttaagagat
   101401 caatcgtatg tttatcaatt gaaattttcc gatttgaagt ctcgtttttt gtttttcccc
   101461 attcatttaa tttataattc catgttttat taatagtgat gatttgatct tcgaaatcga
   101521 tatcttccca tgttaatcct agaagttcag caaatcgcat tccagtagca ccagcaacta
   101581 acacgagcat aggagaagag tactgtgcag aaaggttttc ttctacgagt ttcattaaag
   101641 atttaaattg atcataatct aaatatttat cttcttttgg cttcaatgaa tcttttcctt
   101701 tgattactgc ttttctagtt ggatcaaacg gaattaatcc ttcttctacg gcatctttca
   101761 acgatgctct aatatggtta ttaaacttaa taacggtgga ttttacatgg tctttcgcat
   101821 acttatttaa aaagcgttga tatccaactc tatctaaatc agaaatcaat actgctggca
   101881 tatatttctt tatattcatt aacgtatctt cgtattttct ataagtgata ggagatactg
   101941 ttccttcttt gtagagttgc atccaatctt caaagtaatc tgataataat agatttttgc
   102001 gatccccttt aagaccttta ttcaggttat attctaattc gttggcggca tcttttgctt
   102061 cgccttttgt cttgaatcct gattttctta tcttcgtgta ttttccgtca tcttttttgt
   102121 atgaaatttc gtattgccaa ctatttccac gtttgactaa tcttgccata attgaatcta
   102181 ctctctttct ctgatacaat aggcactgta aagaagccta ttgtgtaggt ttgtttttaa
   102241 cgcgtccaca aactttggcg agggaggggg cgtgtttttt atttatactc tgttgttcct
   102301 gcttcgccaa tcggctcagc tgtaaaatca tcagtaaatt gtttagacgc attaagttta
   102361 aatgtactta aatttggttt atttttagaa agtaaataga aaaccccaga tgttgtcgtt
   102421 tcaggttgaa ctaaagaatt agaattttcg tcatataaga aatattctcc agctgttcta
   102481 ctatattcca taccgtctcc atctattaaa gatgcctgat acgaagacaa agagaaaggt
   102541 gatgttgctt tattttttaa cttgtaagaa atagtagtac gatagtattt ttctggaaaa
   102601 ttttctattc cactaaagtt atattcggca tttgtaaaat tatcttcgtc atttgttacc
   102661 tgttcagttt ttatagaaga aattgtatat tctaggatac caacaactgg caaggttttt
   102721 tccttagaag cattgtcgat agattcgcta ttttcttcag tgtcatttga ctgcgatgct
   102781 atttgttttt taagctcttc attttctttt tttaaactat tgattgtttt ttgagtttca
   102841 ctattagttg cattatttga aggcgaacta catgcaccca aagttaaaat actagtacaa
   102901 ataaatccta atattaagtt ttttttcatt tttaattcct cacttctatg atattatttt
   102961 tatgtagaat cttagaaatg agattttagt ccgtgttgca gcacgggctt ttttctttat
   103021 aactttttag aggttatagg caagatagtg ggacacaaaa atatattatt gtattccgta
   103081 tttagaaaat cctaattgaa cttcaccgga agttttttgc tgtgttgtac gcaatgcttc
   103141 ttcttcagac attccattct gtactttcca tgcaacaggc gacatcccgt atttgttaac
   103201 aaaatcagta agtgataaag tgtcagcgtc ttgttgagcg cttgtttgtt gggcttctgt
   103261 attttgttga gatgcagctt gttgttcttt ctgatcttga ctgataatat tgccagcatc
   103321 atctgtagtc aatccatttt cataaagggc cacgccgaaa gcttcccact ctttgttgga
   103381 ccaatttgca cgatcagctg gagttgactg taaagtgcgt tgtttcatct gttcatatgt
   103441 ttcttcttga ggtgcggttt ggattgtatc ctgactggag cttataactg ttgggctagg
   103501 tttcgccgta gcttggttgg agcttgtaac tgttgaacta gaatctgttt tagatgtaga
   103561 tttgctagta gatgaactga tttcagttgt ttcttttgtt ttacttatat ttgtttcttg
   103621 gttagaagtg gcatctgttg attcagcttt tttattattt gaacaagctg aaagtagcag
   103681 agcagtactt aacaacaaca taacgctaac ttttttcatt ttataattcc tctttctcgt
   103741 tgtaatatgt gtgctaacac gggctttttt atataagaaa acgataagcg ctttctggaa
   103801 gaccgtaaag attctttaat tcctcgattt ttttaggata ttgatcatta tcttctttat
   103861 aaagagaaac aatgagatta gcagcaaagc aattagcttc gctttcagat ttgcttctag
   103921 atgttcttgt tgatacataa tagctggata agccacgatg aaaaatagcg tgacctaatt
   103981 cgtgagcgca aatgtagaat ctttcctcag agtctcgcag ttcatcattt aaaaagatta
   104041 tcgcacgatc tctaatttct tgaaactgcc ccttgggatt ctcgataaaa ggaacgtatt
   104101 gaattttaat gcccatcttt tcacaaatat aaaaaggatt agcggactgg tatttccgct
   104161 tcaactcctc gactaaatta atcgtatcca tctccataag ctcacatctt tttgcctttt
   104221 tctttgtctt ctttcacaat atcccagaaa gtcgctatca ggatatcttt tacgcgctgt
   104281 atttgttcgg gtgtcaatgt ttccccacca taagacatat taacatttga gtctagtagt
   104341 ttatcaagtt caaccacttc ctcttttgta gcccatttgg gaacattatt atttcccaat
   104401 aaataatcag ttgtgacacc aaaataatca gcaactttct ttaagttctc agattttggc
   104461 gaggctttat cccatcttct tatttgtcca ttagagatgc ccacctgtct ttctacttct
   104521 gctatagtca catgcttttc gtctgctaat tctttaatct tagtaactaa actcattatt
   104581 atcaaccttt caaagctgaa aagaaaataa atagcttaaa agttatattt ttggttgaca
   104641 attagctttt aagctagtat atttaattcg taagctaaat tgttagctaa ataagagcaa
   104701 caaaaaactc tactaattta aaacattctc tcggtcgcca aacttagaaa tgttatttta
   104761 gaggcttttt ataagtctta tttaactatg tattcataat agcttaaaag ctaataggtg
   104821 tcaacgattt agctaatttt ttagcttaca aattattttt tagaaaggag ctatttttat
   104881 gtctgagaat ttagacttaa aaattcgagc ggagatgaga aaaagaagaa tgactttcaa
   104941 agaactagct gcgcttgttg gtatttcagg agcttattta tcagatatcc taaacggcaa
   105001 tcgtgatggt aaaaaagctc aagaacacat agaaactgta aaaaaaatat taggtattca
   105061 ataggaggaa cagctaatgc aatatctaga agcgaaaatc ccagttccag aaggctatgt
   105121 aattatctct caagtggatt acgaggagtt aaaaaaagct gatgatactg gtagatggat
   105181 gacgttgcca gaagtgctag aacggattaa cagaaaatat gattggttta cttctagagt
   105241 tttaaagaac ccaagatata gaaatattat cgatatcgag aaaaacaaaa atggatttgt
   105301 ctattatcca gttgaaggaa gagacacata tctattttta agaagtaaaa cacttgaatt
   105361 tttagaaaca aatttttcgg aaatcttaag gaggcaagcg gatgggaaaa tttaatagag
   105421 cgctagt
//
LOCUS       DAYYFU010000004        95323 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000004 DAYYFU010000000
VERSION     DAYYFU010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 95323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.004"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            180..494
                     /locus_tag="ACHA34_000323"
     CDS             180..494
                     /locus_tag="ACHA34_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8791004.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            515..892
                     /locus_tag="ACHA34_000324"
     CDS             515..892
                     /locus_tag="ACHA34_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8791005.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            902..1675
                     /locus_tag="ACHA34_000325"
     CDS             902..1675
                     /locus_tag="ACHA34_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791006.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            1697..3100
                     /locus_tag="ACHA34_000326"
     CDS             1697..3100
                     /locus_tag="ACHA34_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8791007.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3282..4466
                     /gene="mobT"
                     /locus_tag="ACHA34_000327"
     CDS             3282..4466
                     /gene="mobT"
                     /locus_tag="ACHA34_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8791008.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            4463..4753
                     /locus_tag="ACHA34_000328"
     CDS             4463..4753
                     /locus_tag="ACHA34_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791009.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            4750..4971
                     /locus_tag="ACHA34_000329"
     CDS             4750..4971
                     /locus_tag="ACHA34_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791010.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5013..5792
                     /locus_tag="ACHA34_000330"
     CDS             5013..5792
                     /locus_tag="ACHA34_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFM8791011.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            5853..6353
                     /locus_tag="ACHA34_000331"
     CDS             5853..6353
                     /locus_tag="ACHA34_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8791012.1"
                     /translation="MEMKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEE
                     YAIHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENK
                     EDIHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHG
                     VFEYCQ"
     gene            6409..7047
                     /locus_tag="ACHA34_000332"
     CDS             6409..7047
                     /locus_tag="ACHA34_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFM8791013.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7119..7514
                     /locus_tag="ACHA34_000333"
     CDS             7119..7514
                     /locus_tag="ACHA34_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8791014.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            7498..9951
                     /locus_tag="ACHA34_000334"
     CDS             7498..9951
                     /locus_tag="ACHA34_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8791015.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            9948..11975
                     /locus_tag="ACHA34_000335"
     CDS             9948..11975
                     /locus_tag="ACHA34_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8791016.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            11972..12994
                     /locus_tag="ACHA34_000336"
     CDS             11972..12994
                     /locus_tag="ACHA34_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFM8791017.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13011..13940
                     /locus_tag="ACHA34_000337"
     CDS             13011..13940
                     /locus_tag="ACHA34_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8791018.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14254..14301
                     /locus_tag="ACHA34_000338"
                     /pseudo
     CDS             14254..14301
                     /locus_tag="ACHA34_000338"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14317..15117
                     /locus_tag="ACHA34_000339"
                     /pseudo
     CDS             14317..15117
                     /locus_tag="ACHA34_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            15355..15591
                     /locus_tag="ACHA34_000340"
     CDS             15355..15591
                     /locus_tag="ACHA34_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791019.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            15598..17550
                     /locus_tag="ACHA34_000341"
     CDS             15598..17550
                     /locus_tag="ACHA34_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8791020.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(17622..18119)
                     /gene="dfrG"
                     /locus_tag="ACHA34_000342"
     CDS             complement(17622..18119)
                     /gene="dfrG"
                     /locus_tag="ACHA34_000342"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8791021.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            18394..19521
                     /locus_tag="ACHA34_000343"
                     /pseudo
     CDS             18394..19521
                     /locus_tag="ACHA34_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            19619..19807
                     /locus_tag="ACHA34_000344"
     CDS             19619..19807
                     /locus_tag="ACHA34_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFM8791022.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(19865..20218)
                     /locus_tag="ACHA34_000345"
     CDS             complement(19865..20218)
                     /locus_tag="ACHA34_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8791023.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            20749..21171
                     /locus_tag="ACHA34_000346"
     CDS             20749..21171
                     /locus_tag="ACHA34_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8791024.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            21168..21398
                     /locus_tag="ACHA34_000347"
     CDS             21168..21398
                     /locus_tag="ACHA34_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8791025.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            21896..22096
                     /locus_tag="ACHA34_000348"
     CDS             21896..22096
                     /locus_tag="ACHA34_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFM8791026.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            22123..23316
                     /locus_tag="ACHA34_000349"
     CDS             22123..23316
                     /locus_tag="ACHA34_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8791027.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(23384..24949)
                     /gene="guaA"
                     /locus_tag="ACHA34_000350"
     CDS             complement(23384..24949)
                     /gene="guaA"
                     /locus_tag="ACHA34_000350"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8791028.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(25008..25931)
                     /gene="coaA"
                     /locus_tag="ACHA34_000351"
     CDS             complement(25008..25931)
                     /gene="coaA"
                     /locus_tag="ACHA34_000351"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFM8791029.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            26127..26732
                     /locus_tag="ACHA34_000352"
     CDS             26127..26732
                     /locus_tag="ACHA34_000352"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8791030.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            27040..28056
                     /locus_tag="ACHA34_000353"
     CDS             27040..28056
                     /locus_tag="ACHA34_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8791031.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            28197..29498
                     /locus_tag="ACHA34_000354"
     CDS             28197..29498
                     /locus_tag="ACHA34_000354"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFM8791032.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            29544..30206
                     /gene="deoC"
                     /locus_tag="ACHA34_000355"
     CDS             29544..30206
                     /gene="deoC"
                     /locus_tag="ACHA34_000355"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFM8791033.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            30227..30625
                     /locus_tag="ACHA34_000356"
     CDS             30227..30625
                     /locus_tag="ACHA34_000356"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFM8791034.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            30691..31773
                     /locus_tag="ACHA34_000357"
     CDS             30691..31773
                     /locus_tag="ACHA34_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8791035.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            31985..33547
                     /locus_tag="ACHA34_000358"
     CDS             31985..33547
                     /locus_tag="ACHA34_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8791036.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            33540..34673
                     /locus_tag="ACHA34_000359"
     CDS             33540..34673
                     /locus_tag="ACHA34_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8791037.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            34673..35629
                     /locus_tag="ACHA34_000360"
     CDS             34673..35629
                     /locus_tag="ACHA34_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8791038.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            35728..36894
                     /gene="deoB"
                     /locus_tag="ACHA34_000361"
     CDS             35728..36894
                     /gene="deoB"
                     /locus_tag="ACHA34_000361"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFM8791039.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            36918..37736
                     /locus_tag="ACHA34_000362"
     CDS             36918..37736
                     /locus_tag="ACHA34_000362"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8791040.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            37753..38460
                     /gene="deoD"
                     /locus_tag="ACHA34_000363"
     CDS             37753..38460
                     /gene="deoD"
                     /locus_tag="ACHA34_000363"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8791041.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(38758..39444)
                     /gene="gpmA"
                     /locus_tag="ACHA34_000364"
     CDS             complement(38758..39444)
                     /gene="gpmA"
                     /locus_tag="ACHA34_000364"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8791042.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(39616..40197)
                     /locus_tag="ACHA34_000365"
     CDS             complement(39616..40197)
                     /locus_tag="ACHA34_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8791043.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(40330..41013)
                     /gene="rpiA"
                     /locus_tag="ACHA34_000366"
     CDS             complement(40330..41013)
                     /gene="rpiA"
                     /locus_tag="ACHA34_000366"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFM8791044.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            41332..41745
                     /gene="rpsL"
                     /locus_tag="ACHA34_000367"
     CDS             41332..41745
                     /gene="rpsL"
                     /locus_tag="ACHA34_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFM8791045.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            41829..42299
                     /gene="rpsG"
                     /locus_tag="ACHA34_000368"
     CDS             41829..42299
                     /gene="rpsG"
                     /locus_tag="ACHA34_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFM8791046.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            42393..44480
                     /gene="fusA"
                     /locus_tag="ACHA34_000369"
     CDS             42393..44480
                     /gene="fusA"
                     /locus_tag="ACHA34_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFM8791047.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            44652..45839
                     /gene="tuf"
                     /locus_tag="ACHA34_000370"
     CDS             44652..45839
                     /gene="tuf"
                     /locus_tag="ACHA34_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8791048.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            46246..46554
                     /gene="rpsJ"
                     /locus_tag="ACHA34_000371"
     CDS             46246..46554
                     /gene="rpsJ"
                     /locus_tag="ACHA34_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFM8791049.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            46585..47214
                     /gene="rplC"
                     /locus_tag="ACHA34_000372"
     CDS             46585..47214
                     /gene="rplC"
                     /locus_tag="ACHA34_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFM8791050.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            47242..47865
                     /gene="rplD"
                     /locus_tag="ACHA34_000373"
     CDS             47242..47865
                     /gene="rplD"
                     /locus_tag="ACHA34_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFM8791051.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            47865..48155
                     /gene="rplW"
                     /locus_tag="ACHA34_000374"
     CDS             47865..48155
                     /gene="rplW"
                     /locus_tag="ACHA34_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFM8791052.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            48194..49027
                     /gene="rplB"
                     /locus_tag="ACHA34_000375"
     CDS             48194..49027
                     /gene="rplB"
                     /locus_tag="ACHA34_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFM8791053.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            49070..49348
                     /gene="rpsS"
                     /locus_tag="ACHA34_000376"
     CDS             49070..49348
                     /gene="rpsS"
                     /locus_tag="ACHA34_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFM8791054.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            49371..49718
                     /gene="rplV"
                     /locus_tag="ACHA34_000377"
     CDS             49371..49718
                     /gene="rplV"
                     /locus_tag="ACHA34_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFM8791055.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            49732..50388
                     /gene="rpsC"
                     /locus_tag="ACHA34_000378"
     CDS             49732..50388
                     /gene="rpsC"
                     /locus_tag="ACHA34_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFM8791056.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            50391..50825
                     /gene="rplP"
                     /locus_tag="ACHA34_000379"
     CDS             50391..50825
                     /gene="rplP"
                     /locus_tag="ACHA34_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFM8791057.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            50815..51003
                     /gene="rpmC"
                     /locus_tag="ACHA34_000380"
     CDS             50815..51003
                     /gene="rpmC"
                     /locus_tag="ACHA34_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFM8791058.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            51028..51294
                     /gene="rpsQ"
                     /locus_tag="ACHA34_000381"
     CDS             51028..51294
                     /gene="rpsQ"
                     /locus_tag="ACHA34_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFM8791059.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            51352..51720
                     /gene="rplN"
                     /locus_tag="ACHA34_000382"
     CDS             51352..51720
                     /gene="rplN"
                     /locus_tag="ACHA34_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFM8791060.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            51757..52065
                     /gene="rplX"
                     /locus_tag="ACHA34_000383"
     CDS             51757..52065
                     /gene="rplX"
                     /locus_tag="ACHA34_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFM8791061.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            52093..52632
                     /gene="rplE"
                     /locus_tag="ACHA34_000384"
     CDS             52093..52632
                     /gene="rplE"
                     /locus_tag="ACHA34_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFM8791062.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            52651..52836
                     /locus_tag="ACHA34_000385"
     CDS             52651..52836
                     /locus_tag="ACHA34_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFM8791063.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            52872..53270
                     /gene="rpsH"
                     /locus_tag="ACHA34_000386"
     CDS             52872..53270
                     /gene="rpsH"
                     /locus_tag="ACHA34_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFM8791064.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            53302..53838
                     /gene="rplF"
                     /locus_tag="ACHA34_000387"
     CDS             53302..53838
                     /gene="rplF"
                     /locus_tag="ACHA34_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFM8791065.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            53999..54355
                     /gene="rplR"
                     /locus_tag="ACHA34_000388"
     CDS             53999..54355
                     /gene="rplR"
                     /locus_tag="ACHA34_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFM8791066.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            54376..54876
                     /gene="rpsE"
                     /locus_tag="ACHA34_000389"
     CDS             54376..54876
                     /gene="rpsE"
                     /locus_tag="ACHA34_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFM8791067.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            54891..55070
                     /gene="rpmD"
                     /locus_tag="ACHA34_000390"
     CDS             54891..55070
                     /gene="rpmD"
                     /locus_tag="ACHA34_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFM8791068.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            55114..55554
                     /gene="rplO"
                     /locus_tag="ACHA34_000391"
     CDS             55114..55554
                     /gene="rplO"
                     /locus_tag="ACHA34_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFM8791069.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            55554..56849
                     /gene="secY"
                     /locus_tag="ACHA34_000392"
     CDS             55554..56849
                     /gene="secY"
                     /locus_tag="ACHA34_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFM8791070.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            56911..57558
                     /locus_tag="ACHA34_000393"
     CDS             56911..57558
                     /locus_tag="ACHA34_000393"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFM8791071.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            57749..57967
                     /gene="infA"
                     /locus_tag="ACHA34_000394"
     CDS             57749..57967
                     /gene="infA"
                     /locus_tag="ACHA34_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFM8791072.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            58000..58116
                     /gene="rpmJ"
                     /locus_tag="ACHA34_000395"
     CDS             58000..58116
                     /gene="rpmJ"
                     /locus_tag="ACHA34_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFM8791073.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            58134..58499
                     /gene="rpsM"
                     /locus_tag="ACHA34_000396"
     CDS             58134..58499
                     /gene="rpsM"
                     /locus_tag="ACHA34_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFM8791074.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            58527..58916
                     /gene="rpsK"
                     /locus_tag="ACHA34_000397"
     CDS             58527..58916
                     /gene="rpsK"
                     /locus_tag="ACHA34_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFM8791075.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            58996..59934
                     /locus_tag="ACHA34_000398"
     CDS             58996..59934
                     /locus_tag="ACHA34_000398"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFM8791076.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            59962..60342
                     /gene="rplQ"
                     /locus_tag="ACHA34_000399"
     CDS             59962..60342
                     /gene="rplQ"
                     /locus_tag="ACHA34_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFM8791077.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            60614..61453
                     /locus_tag="ACHA34_000400"
     CDS             60614..61453
                     /locus_tag="ACHA34_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8791078.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            61429..62298
                     /locus_tag="ACHA34_000401"
     CDS             61429..62298
                     /locus_tag="ACHA34_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8791079.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            62288..63085
                     /locus_tag="ACHA34_000402"
     CDS             62288..63085
                     /locus_tag="ACHA34_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8791080.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            63288..64034
                     /gene="truA"
                     /locus_tag="ACHA34_000403"
     CDS             63288..64034
                     /gene="truA"
                     /locus_tag="ACHA34_000403"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8791081.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(64367..67036)
                     /locus_tag="ACHA34_000404"
     CDS             complement(64367..67036)
                     /locus_tag="ACHA34_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFM8791082.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(67274..67462)
                     /gene="rpmB"
                     /locus_tag="ACHA34_000405"
     CDS             complement(67274..67462)
                     /gene="rpmB"
                     /locus_tag="ACHA34_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFM8791083.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            67732..68094
                     /locus_tag="ACHA34_000406"
     CDS             67732..68094
                     /locus_tag="ACHA34_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8791084.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            68146..69828
                     /locus_tag="ACHA34_000407"
     CDS             68146..69828
                     /locus_tag="ACHA34_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFM8791085.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            69945..71981
                     /gene="recG"
                     /locus_tag="ACHA34_000408"
     CDS             69945..71981
                     /gene="recG"
                     /locus_tag="ACHA34_000408"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFM8791086.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            72030..73031
                     /gene="plsX"
                     /locus_tag="ACHA34_000409"
     CDS             72030..73031
                     /gene="plsX"
                     /locus_tag="ACHA34_000409"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFM8791087.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            73153..73395
                     /gene="acpP"
                     /locus_tag="ACHA34_000410"
     CDS             73153..73395
                     /gene="acpP"
                     /locus_tag="ACHA34_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8791088.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            73663..74667
                     /locus_tag="ACHA34_000411"
     CDS             73663..74667
                     /locus_tag="ACHA34_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8791089.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            74668..75612
                     /locus_tag="ACHA34_000412"
     CDS             74668..75612
                     /locus_tag="ACHA34_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8791090.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            75612..76574
                     /gene="opp4B"
                     /locus_tag="ACHA34_000413"
     CDS             75612..76574
                     /gene="opp4B"
                     /locus_tag="ACHA34_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8791091.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            76601..77515
                     /locus_tag="ACHA34_000414"
     CDS             76601..77515
                     /locus_tag="ACHA34_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8791092.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            77541..79322
                     /locus_tag="ACHA34_000415"
     CDS             77541..79322
                     /locus_tag="ACHA34_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8791093.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            79670..80356
                     /gene="rnc"
                     /locus_tag="ACHA34_000416"
     CDS             79670..80356
                     /gene="rnc"
                     /locus_tag="ACHA34_000416"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFM8791094.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            80376..83957
                     /gene="smc"
                     /locus_tag="ACHA34_000417"
     CDS             80376..83957
                     /gene="smc"
                     /locus_tag="ACHA34_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFM8791095.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            83966..84775
                     /locus_tag="ACHA34_000418"
     CDS             83966..84775
                     /locus_tag="ACHA34_000418"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8791096.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            84786..85784
                     /gene="ftsY"
                     /locus_tag="ACHA34_000419"
     CDS             84786..85784
                     /gene="ftsY"
                     /locus_tag="ACHA34_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFM8791097.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            85971..88238
                     /gene="gshAB"
                     /locus_tag="ACHA34_000420"
     CDS             85971..88238
                     /gene="gshAB"
                     /locus_tag="ACHA34_000420"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFM8791098.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(88327..88779)
                     /locus_tag="ACHA34_000421"
     CDS             complement(88327..88779)
                     /locus_tag="ACHA34_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFM8791099.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            88929..89879
                     /locus_tag="ACHA34_000422"
     CDS             88929..89879
                     /locus_tag="ACHA34_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8791100.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            89872..90831
                     /locus_tag="ACHA34_000423"
     CDS             89872..90831
                     /locus_tag="ACHA34_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8791101.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            90828..91583
                     /locus_tag="ACHA34_000424"
     CDS             90828..91583
                     /locus_tag="ACHA34_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8791102.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            91606..92559
                     /locus_tag="ACHA34_000425"
     CDS             91606..92559
                     /locus_tag="ACHA34_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8791103.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(93203..93499)
                     /locus_tag="ACHA34_000426"
     CDS             complement(93203..93499)
                     /locus_tag="ACHA34_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFM8791104.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            93854..94210
                     /locus_tag="ACHA34_000427"
     CDS             93854..94210
                     /locus_tag="ACHA34_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8791105.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            94220..95119
                     /locus_tag="ACHA34_000428"
     CDS             94220..95119
                     /locus_tag="ACHA34_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8791106.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        31367 a        16965 c        20069 g        26922 t
ORIGIN      
        1 tttaaatata gtaggaatcg gacgctcagt acggagcgtc tattttttta cccaattttg
       61 aaaggaagtg aacttatgaa aataaaaaat caagaatcaa aaggtcgctc tccccccttt
      121 aaaaccatca cacaaataag attcattcaa acataaaaac agaaaggata ggtaaaaata
      181 tggaacttaa atatgtcatt cccaatatgg aaaaaacatt tggacaatta gaatatgctg
      241 gtgaagggaa cattgaacaa cgtcgaataa atggcagacc aaccattctc tctcgtagtt
      301 acaatttata ctcaacgatt caacgagcag atgatattgt tgtcattctt ccctccgaag
      361 ctggcgagaa acattttgaa tccgacgaaa aagtcaaact catcaaccca catattaccg
      421 cagaaggtta taaaattggc aatcgaggat ttaccaatta cattatgcac gcagacgata
      481 tggtgaaagc ataagaaagg aaaggtgaaa aaatatgaga ttagcagaag gtattgtgat
      541 tgaaaaagac cagacatttg gcacattgaa attttccgcc ttgcgtcgtg aggttcgtat
      601 ccagaatgaa gatggtacgg tttccgaaga aattaaagaa cgtacctatg acttaaaatc
      661 aaaaggacaa ggacgcatga tacaagtcag tatccccgct tctgttcctt tgaaagaatt
      721 tgactacaac gcagaagtgg aattaatcaa tcctgtggca gataccgtag cgacggctac
      781 ttttcgaggg gcagacgttg attggtacgt gaaagcagac gatcttgtat taaaaggaca
      841 gacaaaacct catcagaaat ataaaaaaga agcgtccgaa agtgaaaaat aggtgttgct
      901 tatgacaaac acaccattga ttttgaaaga aattccgaag gatgaagcca tttcttttat
      961 tcggcaatac cattattcaa agatactccc tagattgtgt aaatactttc tagggatttt
     1021 ttcagaagaa aaattattag gcgttgtgga attaggctgg ggtacgcaac ccctacaaac
     1081 catacgcaaa ctctttcccg acagttccct tcaaaccaca gattatttag aaattggaaa
     1141 gatgtgtttt cttccagaaa tgaatcaaac caattatttt ggcagtcaag ctctttcagc
     1201 tctaattaag tggttgaaag aacatacgga ctgtcatttt ttatatacac tggcagatgg
     1261 aattgaggga aaatgtggct atgtctatca agcgtccaac ttcttttact gcgggtattt
     1321 caagacaagt gtctatcgtg ataagcagtc atgggaaaag atacaccctc gaagtgctcg
     1381 ccttttatta gaggaaaatg ctcgctttga acaggtagag aaaaagcatt ggctttccca
     1441 ggcattttgc gaatacaaag ggattgagaa aatcaatgga cgcatgtttc gctatctcta
     1501 tccgttgaca aaagaagcga aaaaactgtt aggacacact ctctatcgac gtcattatta
     1561 tccgaaagaa aaaaatttac gctttgaaaa gcgaatcgct tatcagaaat atgaagccat
     1621 ttctcaacca acttttgata aacaggctcg tatctacaat acacaattat tttgataggt
     1681 ggaaaggagg atttttatgc cattgcttca tgacagagga gagcgaatca aagccaacga
     1741 taagcacttg gtctatcatt ttgtactaat ttcattgctg gtagtttttc tagtcgtcct
     1801 actccctttt cattggagag cgttacaatc ggtgaactgg caagcagttc agttagatac
     1861 atttttctat caacttcata cgccatactt ttggagcagt attctcactg ctctttttgt
     1921 atgcggactt atcagctggc tttattatca ctatcgtata gattctataa aacagttaga
     1981 gcaccgtcaa aagttagctc gtatgatttt agaaaatcat tggtatgaag ccgaagaaac
     2041 tcaatcagag ggattcttta aagatttacc cagcaagaaa accaaacaac gaatccgcca
     2101 ttttcctaaa atatattatc gaatgaaaga tggactaatc catattcatg tagaaatcac
     2161 cttagggaaa tatcaagatc agcttttgca cctagaaaac aaactagaaa caggattgta
     2221 ttgtgaatta gtggaaaaaa cgttgaaaga ttcttatgta gagtatgtcc tactttatga
     2281 tactattggc aatcgaattg gcattgaaga cgtggtagct caagatggac gtgtcaaatt
     2341 gatggaatcg gtctattggg agttcgattc tctccctcac atgttaattg ctgggggcac
     2401 tggtagtgga aaatcctatt ttattctcac attgattgaa tctctcctgc atacaaatgc
     2461 aaagctttat attcttgacc ctaaaaatgc tgatttggca gacttagggg cggtcatgaa
     2521 taatgtctat taccgtaaag aagatatgtt ggcatgtatc aatcaatttt acgaagatat
     2581 gattgatcgc agtgaaacca tgaaacaaca ccctaactat aaaacaggag aaaattatgc
     2641 ttatcttggt ttgtctgcca attttcttat ttttgatgaa tatgtggcaa tgatggatat
     2701 gctaggtcgt gaaagtacca ccgttattca taaactcaaa caaattgtaa tgttaggacg
     2761 acaagcaggc ttctttttaa ttcttgcctg tcaacgtcca gacgcaaaat attttagtga
     2821 tgggattcgg gacaacttta acttccgtgt ggctttgggt cgtatgtctg aaatgggctt
     2881 tggtatgatg ttcggaagcg atacacaaaa acaatttttc ttaaaaccca tcaagggaag
     2941 aggttacgtg gatacaggaa aaagtgtgat tagtgagttt tatacacccc ttgtaccaaa
     3001 aaggtacgat ttcttaggag aaataggaaa agtcattcaa aaaaagcaat ctgagcctgt
     3061 accgcacgaa gtgaaaggta caggttcaga cttgctttga ttggtgtggc gttagccacg
     3121 ccaatccttt agccccctct atctaacagg ggggcacaaa tacaactgaa aacaggcaat
     3181 tctcatcaac caatgttgat ggtacaaagg attgcggaaa atatgaaagg tgtaaactca
     3241 agggtcatag tttacatctt ttttagattg gaggtttgcg aatggaaaac gaaaaaattt
     3301 ggattaagga attgaaagaa aagcgactgg cttatggcgt ttctcaaaat aaactggcgg
     3361 ttgcttctca tattaccaga ccttacctat cggatattga aacaggcaag gcagtcccta
     3421 ctcaacaggt gaaagaagat ttactgaacg ctttggaacg cttcaatccc gataacccat
     3481 tggagatgtt attcgactat gtacggattc gattccctac caatgatgtg gctcaaatta
     3541 tcggtgaggt tttacgtttg aatatggatt atatgctaca cgaagacttt ggctattatt
     3601 cttatcccga acactatcgt ttcggtgata ttgttgtcct agtctctcat gatgtaagta
     3661 aaggtgtttt attagaactg aaaggaaaag gctgtcggca atttgaaaac tttctattgg
     3721 ctcaacatcg gagctggtat gatttctttc aagattgcat ggaacataaa ggtattttca
     3781 aacgcttgga tttagctatc aacgataaaa cagggatttt gaatattcca gatttggcaa
     3841 agaaatgtaa acaggaagaa tgcatttccg tctttcgaag ctttaaaaat tatcgcagtg
     3901 gtgaattggt tcaccgtgat gaaaaaccat acatgggaaa cacactttat attggctcat
     3961 taaaaagtga agtctatttc tgtatatatg agaaagatta tgagcaatac gtgaaaaatg
     4021 atgttccctt agaagacgca gaagtaaaga atcggtttga aattcggtta aaaaatgacc
     4081 gagccaccca agccatgaaa gatttattgg cacatcaaca ggcggaaaaa acagcctttg
     4141 aaattatcaa tcgctatatc cgctttgcag ataaagacga cactaaacgt cggagtgatt
     4201 ggaaaactaa tgaacgttgg gaatggttta tcggtaaaaa tcgtggagca ttacgcctaa
     4261 ccacacagcc agagccttat tcttttgaac gaacattgaa ctggttacat catcaagtcg
     4321 cccctacatt aaaaatcgca agcattttag atgttctcaa cggtacaacc attatctcca
     4381 ccatgattca ggaagccaaa ctgacagaaa aacatgagaa attaattgaa caacaacatc
     4441 tagccatgga ggatttgatt acatgagcct tttaacaaaa gaattaaaga agttagggtt
     4501 tcagtgtggc attgagtttc aagcgtatat acaaaataca gggaaatata cgtctctcat
     4561 tattgaggga aaacggcaag ctggtgatac catctatacc tatgattttt acaaggtgag
     4621 attttataat aattatacca atcgagtaac cgtctatgga gagcatttaa ccccttttca
     4681 attattaagg cgtgtaaaaa gctatatcta ttatcgagaa aaatatctta aggaaaggag
     4741 gacaatcaca tgaattttgg gcagaatttg tacaactggt tcttggataa tgcacagtca
     4801 ttggtattgt tagcgattgt tgtcattgga ctgtttttag gttttaaacg agaattttca
     4861 aaactcattg gcttcttgat tattgctcta atcgccgtcg gacttgtgtt caatgcagct
     4921 ggcgtgaaag atgtgttatt aaacttattt aaccgaatca ttggtgctta acagatattt
     4981 ttcatgataa aataggaagt atatattttg ttatgaaagg atttatgaat atgtttacaa
     5041 atgatcaatt taaatctctt ttaattaaaa cattagaaag aaagagctct tcaaattatt
     5101 atgaaggagg taacgagatt gtttctaaaa tgaaaatatc agaggtaact ttagatgaga
     5161 ctgacgattt tacttattat aaaggatata aaaagggctg gaatacgctt tgcacatatc
     5221 ttaaaataag agttcctttt gatgatttag acttttttga aagtcataaa gatttgatta
     5281 cccagacagc aagctccata tatgataaac aaggtgataa tgtattagtg gatactattt
     5341 tagtaccact tccagagaat tacgaagtaa ttaactttag tcagttgaaa attagtgatg
     5401 tggtttcaca agctattgaa gacgctgagt cttttatgag taatggtgaa tatcaacgtg
     5461 cctttgacag agttcacact gcttttcatg gatatttaat tgaaatacta aaaaaatatg
     5521 aaattacagt tcctagagac gaaaatctat ctaaattata tagtaggata caacaattaa
     5581 ttgaaaaaga aattcaacct actgaacttg ctgatatagt gaagacaacg ataaggtcat
     5641 caaatggaat gattagctct cttaatgaag caagaaatag gcattcactt gcacacccta
     5701 atacaaatat tattggaaag agagaagcaa aactaataat aggtatttca agtacagtta
     5761 cagattatat tagcggatac cttgataagt agcgtcatta actgattgct acttttttta
     5821 tgactatttt tataaaaagg atgtgataag aattggaaat gaaagtctat gtggcaaatt
     5881 taggacggta taatgaaggt gaattagtcg gagcatggtt tactccacca attgatgagg
     5941 aagaaatggc agaaagaatc ggtttaaatg aggactacga agaatatgca atacatgatt
     6001 ttgagcttcc ttttgatgtg gacgaataca cgccgattag tgaaatcaac cgtctatgtg
     6061 aagccattca agaaatcgaa ggtacaccta tttataatga actcaaagaa attcaaggca
     6121 tgtggtttag tagcttggaa gaattactgg aaaacaaaga ggatattcat tgttatagtg
     6181 attgtgattc gatggaagat gtcgcccgtt attacgtgga agaaacagga cagcttggtg
     6241 aagtcccttc caatttacaa aattatattg actaccaatc tttgggacgt gatatggaaa
     6301 tagagggtaa ttacctagtt acctctcatg gggtatttga atattgtcag taagtttaac
     6361 aatccgtgtt ataattaaat taataaatag cacggagagg tataaattat ggtaaatgta
     6421 tttgaaaatc ttaaactatt aaaactagac atgaaagata aaggctgggt gatagattcc
     6481 ttttactttc gttacaaaca gcaaaattat attgtacttg ttaagctatt tgaaaaagag
     6541 gaaaaagttc cagaatatgc tctgttaaaa ctagaatttt taaaggaaaa cgatttttca
     6601 gatatgctag ctgtctatgc taattcggta aaattatata cagacacgaa aacaataaga
     6661 gaatattttg gtatcgaata cagtagtaac ttgggagatg ttttatttca atttagccaa
     6721 actcttgctc gatttattcc gacagaagtt agtgagaaga aaaatgaaga ccaaaaagaa
     6781 gccatgtgtt tctcattaag tcaaagtgat tcagaagatc ccagaaaaaa atattgtttt
     6841 tcagttagaa gaaatccttt aaagggaaat ggtgagctgg gaaaacgaag tccttttaat
     6901 gacaataaaa cgagattgta tagacccagc ttatatgaac gattagggtc agataccaat
     6961 ttaagtttca gatattcaat gaatccagat gatgaagaaa ctgatgaagg tattattgca
     7021 aaatggacaa aaaataaaat tgaatagtta aagcattgaa agagcaatca cctattttga
     7081 ttgttctttt tctattttta cggaaaggac gtgaagacat gaaaaaaata agaagttaca
     7141 ccagtatttg gtcagtagaa aaagtgattt atgcgattaa tgatttacaa ctccctttcc
     7201 caattacctt tacgcaaatg acatggttta tcgtttcttt gtttgtcgtc atactctttg
     7261 gcagtttacc accactttct ctgattgacg gagcactact caaatactta gggattccca
     7321 ttgttctcac tgggttcatg tgccaaaaga cctttgacgg aaagaaaccc tatggatttc
     7381 taaagtctat cattacctat gctctacgcc ctaaagtaac ctacgctggc aaacaaatac
     7441 gattaaaaaa agaaacaatg aatgaatcga ttacgattgt aaggagtgaa caactagatg
     7501 gcatatccga ttaaatatat ggaagataat ctcgtattta atcatgatgg cgaagtattc
     7561 gcttattatg aattaattcc ctataattac agttttttaa gttccgatga aaaaattcaa
     7621 gttcatgata atttctgtca attgattgcc caaaatcgtg atggcaagat tcatgctctg
     7681 caactagcaa ccgaatcaag tattcgagcc acacaggaac aatcgaaaaa agaaatcact
     7741 ggacgattaa aagagatagc ttacgaaaaa atagatgaac agaccgaagc gttaatttct
     7801 atgattggtg acaatcaagt agactatcgc ttttttattg gttttaaact ctacctcaat
     7861 gaacaggaag tatccgtaaa aaatgttggg aaagaaattc aaaaagcatt tgcggaattc
     7921 tttcatggtg tgaatcacaa attaatgggc gattttgttt ctatgcctat ggcggaaatc
     7981 gaacgctttt ctaagatgga acagttgtta gaaagtaaat tatccagacg ctttcagatt
     8041 cgtaaattgg agaaagatga ttttggttat attattgaac atctttacgg gcaaacaggt
     8101 gtcccttttg atgactacga ctaccattta ccgaaacgat acgaagataa aaaagtgttg
     8161 gtaaagaaat acgatattct caaacccact cgctgtttaa tcgaagaaaa tcaacgctat
     8221 ttacggattg aacaggaaga taccactact tatgtggcgt attttacaat taatagtatt
     8281 gttggtgaat tggatttccc taatagtgaa attttttatt accagcaaca gcagtttact
     8341 ttccccgttg ataccagtat gaatgttgaa attgtcgcca ataaaaaagc cttatctacc
     8401 gttcgcaata aaaagaaaga actgaaagat ttagatagcc acgcatggga aagcgacaac
     8461 gaaaccagta ctaatgtggt ggacgcttta gaatctgtcg atgaattaga aagtaattta
     8521 gaccaaacca aagaatctat gtacaaacta agctatgtgg tacgggtatc agcaaagaat
     8581 ttggacgagc tgaaacaacg ttgtgatgaa gtaaaagatt tttatgatga tctgtctatc
     8641 aaattagtac gaccttttgg cgatatgcta ggcttgcata atgaatttat tccctcaagc
     8701 aagcgatata tcaatgacta tattcaatat gtcacgtcgg attttttggc tggtcttggt
     8761 ttcggtgcta cacaaatgat tggtgaaaat tctggtattt acgtcggtta caatctcgat
     8821 acaggcagaa atatttatct ccaacctagc cttgcaagtc aaggaattaa aggctctgta
     8881 acaaatgcat tagcgtccgc ttttattggt tcattaggtg gcggaaaatc attctctaac
     8941 aatatgctgg tctattattc agtactcttt ggcggacagg caaccattgt tgacccaaaa
     9001 gctgaacgtg gtgaatggaa agaaacctta cctgatattg cccatgaaat caatattgtt
     9061 aatctcacga gtgagaacga caataaaggg ttacttgacc cttatgtgat tttaaaactc
     9121 ccgaaagatt cagaaagcct tgccattgat attctcacgt tcttaacagg tatttccagc
     9181 cgagatagcg agaagtttcc tgtcctgcgt aaagctattc ggagtgtaac tcaaagcaaa
     9241 caacgagggt tgttattggt gattgatgaa ttgcgaaaag aagatacgcc catcagcaat
     9301 agtattgctg accatatcga aagctttgtc gattatgatt ttgcccatct gttattcagt
     9361 gatggcagtg tcacccaatc cattagcttg gaaaaacaac tcaatattat tcaagtggca
     9421 gatttggtct tacccgattc caatagctct tttgaagaat acactaccat ggaattatta
     9481 agtgtggcaa tgctgattgt cattagtacc tttgctttag actttatcca ctctgaccgt
     9541 tccattttta aaattgttga ccttgatgag gcttggagtt ttttacaagt ggcacaagga
     9601 aaagctctat ctatgcgatt ggttcgagca ggacgtgcca tgaatgcagg ggtctatttc
     9661 gttactcaaa acgcggacga tttactagat gaaaagatga agaataatat tggcttgaaa
     9721 tttgcctttc gttccactga cctagtggaa atcaaaaaga ccttagaatt ttttggtgtg
     9781 gacaaggaag atgaaaacaa tcagaaacgc ttacgtgaat tagaaaatgg acaatgttta
     9841 gtttcagaca tctatggtcg tgtaggggtt atgcagtttc acccgatttt tgaagagttg
     9901 cttcatgcct ttgataccag accgcctgtg cgaaatgagg tggaagaatg acacaaaaac
     9961 aaaagaaatt tcttcactat agttggataa cgctcctttg tgtgggcgtt attttacttt
    10021 tattaggcac gctgggcaat gcagtacaag cgacaggctt agttgatgaa accattgata
    10081 caagcaatga atactccaag tatggtttga atcaatatca gcttgattat tatgtggaca
    10141 atagctgggg ctggcttcca tggaattgga gtgatggcat aggacaatcg gtgatgtatg
    10201 gattgtatgc cattactaat tttatttgga caatttctct ttatctatcg aacgccacag
    10261 gttatttggt gaaagaagcc tatgccttag atttcattag tcaaacatcc caagcaattg
    10321 gcgaaaacat gcagacgatt gcaggcatta caccgaatgg cttttccagt tcagggtttt
    10381 atgtcggttt cctccttctt tttattctca ttgttggaat ctatgttgcc tatacaggat
    10441 tggttaagcg agaaacgacg aaagtgatac gagctgtttt aaactttgtc gtggtgttta
    10501 ttctatcggc ttcctttatt gcgtatgcac ccgattatat tgcgaaaatc aatgattttt
    10561 ccaaagatgt cagtaccgcc agtctgaata ttgggacaaa aattgttctt cctaacacgg
    10621 atagtcaagg gaaagacagt gtggacatga tacgtgacag cttattctct gttcaagtaa
    10681 agcaaccatg gctattgctt caatatggct ccactgatgt agaagcgtta ggggaagaac
    10741 gtgtggaaag tttattgtct accagtccag atacaaacaa tggtgaagat agagaaaatg
    10801 tagtgattga agaaattgaa gatcatgaca atacctattt aaccctaccg aagacgattt
    10861 ccagattagg aacagtcttc tttttgtttg tattcaatat tggtatctcg atttttgttt
    10921 tccttctaac aggaatcatg attttttcgc aagtgctatt tattattttc gctatgtttt
    10981 tgccaatcag tttcctatta agtatgttgc cgagttttga tggcatgggc aggcatgcca
    11041 ttaccaagtt atttaatgtg attatgacaa gggctggtat taccttgatt attaccgttg
    11101 cctttagtat ttccactatg ctttattcgc tgtcagcaag ttctcccttt ttcatgatta
    11161 tgtttttaca aattgtgacc tttgcaggga tttattttaa actgggcgat ttaatgagcc
    11221 tattcacttt acaaagcaac gactctcaaa acatgggcag acaagtgttt cgcagacctc
    11281 gtcaaatgat gaatcgacaa accagaagat ttcaacgcag tatgagtaag atttttcgtg
    11341 gaaaagcagg gtcaacgaag caaaagacaa tgacaacaaa gcagaatcaa accaataaag
    11401 ctaaccatac tcgtcaaaat gaacggacta caccaaagaa agaaacagca ctttccaatg
    11461 tgaaaaagca acaaggcggt atcgggcaac gattaggtga aaagacagcc catgtattgg
    11521 acacgaaaaa tcgtctagtc aataaaagaa agcaggcaaa acaacagata caggcgacac
    11581 caacccatgt caaatatgcc cttcatcaag gaaaagagaa tgtcactcat aatgtaacag
    11641 actttaaaca atctatggca gataccaaag ctcaacgcaa acaacagcgt atgaaccagc
    11701 aggagcaaaa acgaaagaac attgctcaaa agcgtttgga gctggataaa gcaaaagaaa
    11761 aaagacgaaa agaaaaccca tcatctcctg ttcagaataa ccagcagcat gttcgcaagc
    11821 gtcagcaaac cgtcacgcct gttttgtcac aaaacactaa acgtgaaaat catcagcgac
    11881 cattgaaaaa agagcaaaaa caaaagacac caccgaagca attcaatcag aggaagctaa
    11941 ataaaagaac ggataagcga ggacaacaaa catgaagcta aaaataacag tgatttgtag
    12001 tgtggtcttc tctctcttct tttttctctt attattccta gtcattttct ttgcagatga
    12061 tacggacagt ggcgaaaaca acaaggattc ttctattcca caaggcggtg tgaccgtttc
    12121 accagaagtg ttggcacacc gtccgttgat tgaaaaatat ggtaaggaat atgggattga
    12181 agactatgtt tcttatatct tagccattat gcaagtggaa tcgggcggta cagcagaaga
    12241 tgttatgcaa agttccgaat ctttgggttt accgcccaac agtttgagta ccgaagagtc
    12301 catcaaacaa ggggttaaat atttcagtga attattaacc agtgcagaac aacaaggagt
    12361 ggatatagat tctgttatcc aatcttataa ttatggcggt ggctttttaa attatgtgag
    12421 gagtcatggg aaaaaatata cctatgaatt agccgaacag ttttctaaag agaagtcggg
    12481 tggtcaaaaa gcagactatc ccaatcctat agccatacct gtgaatggtg gttggcgata
    12541 taactatgga aatcagtttt atgttcagtt ggtttcacaa tacctaaccg acacttcccc
    12601 aacagaattt gatgatgaaa ccgtgcaagt cattatggac gaagccttga aatacgaagg
    12661 gtttccctat gtctttggag gggcgtcgcc taccacctct tttgattgta gtggcttaat
    12721 acaatgggtg tatgacaaag ctggcatttc tctccctcgt gtcgcccaag atcagtatga
    12781 cgctacgcaa gaaatctcta tggaagaagc ccaagcaggc gaccttatct tctttcattc
    12841 aacctataat gcaggcacat atgtgactca tgtggcgata tatttagagg gaaatcgctt
    12901 ttatcatgca ggcgacccaa tcggttatgg cgacttaagc agtcgttatt ggcaagacca
    12961 tctgattggg gctagacgtg ttattcataa ctaagaaagg aatgaatcaa atgattataa
    13021 aaatcgagcg aaaagaaaag aaggagaaac caaagaaatc taaaaaaccg agcaagcaaa
    13081 gaaaagttcc gatgattaaa gttggcacgc ataagaaatt aacctttgtt ttatgggttt
    13141 tattgattgg tagtgtcgga tttggaatct ataaaaattt tactgccatt gacactcata
    13201 ctgtccgtga aacagaaatc attaaacagc agattgtcga taccaaccaa gtagaaagtt
    13261 ttgtgaaatc ctttgcgaaa gactactttt cgtggcaaca atcccaagaa gccattgata
    13321 aacgaaatga aaaattgacc cattatttga cggaagaatt gcaggtatta aatgaagaaa
    13381 tgattcgtaa ggatattcct actagttcta gtgtgaacga tatacaggtg tggcaggttt
    13441 ctcaagtgaa tgagaacacc tttgaggtac tgttttctgt tgaacaagtt attaccgaag
    13501 acaaagacaa ggaaactata tcgtctagct ttcatgtggt tgtccatata gatgaatcag
    13561 ataatatggt tattatcaaa aatccgacaa tgagtaagaa acctcaaaaa tcggactacc
    13621 aacccaaaca gctagaaagt gaccatacag tagatacaga aacgatggac gaaatcacta
    13681 gctttttaga aacgttcttc cagctctatc ctaccgccac agaaaaagaa ttgacttact
    13741 atgtaagtaa tcatgtgtta cctatgatta acaaggaata tgttttcgag gaattggtga
    13801 atcctatctt taccagaaaa gataaccaag taatcgtgaa tgtcgctgtg aaatatttag
    13861 atcaagaaac aaaggcaacc caaatctcgc aatttgagtt gatattagaa aaacaagata
    13921 attggaagat tgtaaaataa caaatattgg tacatgatta cagatacttt gtaatcatgt
    13981 actctttttg ataaaaaatt ggagattcct ttacaaatat gctcttacgt gctattattt
    14041 aagtgactat ttaaaaggag ttaataaata tgcggcaagg tattcttaaa taaactgtca
    14101 atttgatagc gggaacaaat aattagatgt ccttttttag gagggcttag ttttttgtac
    14161 ccagtttaag aataccttta tcatgtgatt ctaaagtatc cagagaatat ctgtatgctt
    14221 tgtataccta tggttatgca taaaaatccc agtgataaaa gtatttatca ctgggatttt
    14281 tatgcccttt tgggtttttg aatggaggaa aatcacatga aaattattaa tattggagtt
    14341 ttagctcatg ttgatgcagg aaaaactacc ttaacagaaa gcttattata taacagtgga
    14401 gcgattacag aattaggaag cgtggacaaa ggtacaacga ggacggataa tacgctttta
    14461 gaacgtcaga gaggaattac aattcagaca ggaataacct cttttcagtg ggaaaatacg
    14521 aaggtgaaca tcatagacac gccaggacat atggatttct tagcagaagt atatcgttca
    14581 ttatcagttt tagatggggc aattctactg atttctgcaa aagatggcgt acaagcacaa
    14641 actcgtatat tatttcatgc acttaggaaa atggggattc ccacaatctt ttttatcaat
    14701 aagattgacc aaaatggaat tgatttatca acggtttatc aggatattaa agagaaactt
    14761 tctgccgaaa ttgtaatcaa acagaaggta gaactgtatc ctaatatgtg tgtgacgaac
    14821 tttaccgaat ctgaacaatg ggatacggta atagagggaa acgatgacct tttagagaaa
    14881 tatatgtccg gtaaatcatt agaagcattg gaactcgaac aagaggaaag cataagattt
    14941 cagaattgtt ctctgttccc tctttatcat ggaagtgcaa aaagtaatat agggattgat
    15001 aaccttatag aagttattac taataaattt tattcatcaa cacatcgagg tccgtctgaa
    15061 ctttgcggaa atgttttcaa aattgaatat acaaaaaaaa gacaacgtct tgcatattaa
    15121 gtagttcagt tttggagtac aaaaagtgaa aatgtaaacc taaatgagta tcaaattgca
    15181 actgaatttg atactcgttt tttattgtta aatcaacgtt tgttgttaat ttacaccaca
    15241 aaatgtaagt taaaatgtat ataaaaatgc gaacgtgaaa taattattgt aggaatgcaa
    15301 attggtgtgt taaaatggtt acaatttact aagcaaaaag gggtgcattt tcctatgcaa
    15361 tttcttgagc caaaaaataa gaacgctaag tctgtggatt gggaaatatc agagcaagta
    15421 agggtgatag ttaagcaata cgctgaatat gcagagcgta ctgaaagcga agcagtggat
    15481 gaattcttat tgaacatttt agacgataag aagtttattg aatggattgc caacaagcga
    15541 agtaataagc ggattgttga aaaaatgggt atcaaagata gagtgggttg atagacaatg
    15601 accaagctta aaagattagc cacgaaggaa gatgaaattg tagatgttaa aattccgatt
    15661 tcaaacgaag aattaaaaga tagggctaaa caatatgatt tattaactcc aaaacgattt
    15721 gcaacaaggt ataacaaaat gttgttcttg ccaacgtctt ttaaatggaa cggttctgaa
    15781 tatcccattc aatataatta ctgcatcaat ccgttttgtt gtaattttgg gaaagaacag
    15841 cacaaattca aagatgtaaa agggaaacca agtcgatata aaatgactgg tagttccaaa
    15901 gataaaggtc ataaaggtat gtactgcaat gacaatccta ttggcagagg tgtatcgcaa
    15961 aactgtacag taaccccttt atctaattgg tctgttgttg aagaaattaa acgccttata
    16021 gaaatcaata gcatccaaga tgttgagcca gattatcaat ttcataaaga aggctgctcg
    16081 gaagaagaat caaccccatt caatgagccg aagcaatttt acaaaagggg gaaaagtagg
    16141 ggcaaatctc aacgctatca atgtaaggcg tgtaagaagt tcactaatgt tctaccaaaa
    16201 agagaagaaa ccactactta tcaccaacaa aagaacacca ttcttccaat gttcgctaag
    16261 atggttgttg gaagggtttc agtcagtcgt acttgtgata ttttaggaat tggagttgga
    16321 acctactatc ataagctgga gtggctgtat aggcgttgtt tggagttttt agaacgatat
    16381 gagacacaac cccttcaaac aaagaaattt aacgagatgt ggcttaatac ggacaagatg
    16441 cactactacc taaataacgt ccgtaagaaa ggacaaggtt caaagaaata tacggggttt
    16501 gaagacctta atatgcaaac gtatatagtt gtatctgctg aggtcttatc aagatatgtg
    16561 ttccgttctg atgttgccta tgattggaat atttcaatgg atgaattaaa tgaagataca
    16621 agaaaattca aagaagacca tctcaatact tttagtcgta aaaatgatag gttagattgg
    16681 tcttattatc cacaagaacc atccgctaac gatagcgaaa ataggaatgc ttatcttcac
    16741 gaattaggga aaattactaa taggagtaga tttgtcgatg gtttaaatgt tgatgctcct
    16801 tatactacaa ctgctcacta ttggttaatt aaacaaatgg ttaatgccga cgaatggcga
    16861 atgattagtg atgatgactt ctctattaga aacgcctttt atagagtatt cacgaaagaa
    16921 ttaaggttga gcgatgcaca tcattttatt tgccaagtta acaagacgaa aagcagaaaa
    16981 caatgcttaa aagaattcgg acaagcaaaa gcagaactgc tcgattgggg agatatcaga
    17041 ggattcaaaa caaagtttct acggactttg gcatctcatt atctaactga attactaaca
    17101 agtcatcaat tccacgaaga ggctattagt aaagatggag aaagatatcg caaatatgcc
    17161 gataacccta tcaaacatcc attagccact aaggataaag gattttattc agttgattgt
    17221 agaacggatt tatctgctct ggaaccaaac gaaatcgcaa agatgctatt aaatgttaat
    17281 gaccattcga ccaatagctt tattcaacag ataagacgtt atatatcatc tcttgaaaga
    17341 ccattaacaa cagctcgtgg tgataagaag agctatattt acgctaactt caatccgaag
    17401 tatgctcaat ttgcgattac aattctaaga acttactaca acttttgtcg cccttttaaa
    17461 tcagcggata aaaaggtttt gacacctgct caaagattag ggattacaga taaacaattt
    17521 gattggaaag atataattta tttcaaatga caaaaagagc ttggagtaat cccaagctct
    17581 tgtgctttat ctttactgtt cttcttcaat taacgcaccc gttaactcaa taagtttttt
    17641 ctttcatata catgaaaata gtagttatac ggatttttat cattctttat ccctttttgg
    17701 gcaaatacct cattccattc ctcataattc acttctggaa aaaaagtatc tccttcgaat
    17761 tcatgatgta tttttgtgat gtacattttc tcaacataag ggaaaaacaa attataaatc
    17821 tgttctcctc cgaaaataaa aatttcttct tcgtttttac ataactcaaa aacatcttct
    17881 attgaatgaa caatttcaca accattaaag gtaaacccct tatctctcgt cagaataata
    17941 tttcttctgt caggtaaggc tcttccgatt gattcaaggt tcttcctacc taatattatc
    18001 ggatgtccct ttgtagtatt tttaacatat tcccagtcct tgggaatcct ccaaggaatg
    18061 tcattctctt tgccaatcac tctattctta tccatcgcag caatcaaaga aactttcatt
    18121 tataacacag cctttttaac taatttacca tctaatttct tatttctatc aaaaaacctt
    18181 ttctcattat cctgcccgtt agttgaataa gcaatttttt ctataagtta tattatcata
    18241 ataccacaaa atcacaaatt ttcattcaat tatcattttc aattgcattt tataaatcat
    18301 tttgtatgta atttggtatg taagttaaaa tgaaaaaagt agtccatttt gaagtacatt
    18361 ttgtcatttt gtactccaaa actgaactac ttacttgcat atatacgcct ttatagtgga
    18421 gtactacatt tacgagattc ggttagagta tcagaaaaag aaaaaataaa agttacagaa
    18481 atgtatactt caataaatgg tgaattatgt aagattgata gagcttattc tggagaaatt
    18541 gttattttgc aaaatgagtt tttgaagtta aatagtgttc ttggagatac aaaactattg
    18601 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
    18661 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
    18721 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
    18781 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
    18841 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
    18901 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
    18961 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
    19021 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
    19081 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
    19141 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
    19201 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
    19261 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
    19321 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
    19381 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
    19441 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
    19501 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
    19561 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
    19621 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
    19681 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
    19741 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
    19801 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
    19861 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
    19921 atagttcttt gtctgtaaag ctatccatgt atttttctac ctgtaacctt cgagtgcttt
    19981 taatcaaatt atctgtaggt aagaaaaatt catcaataga tacatggagt aatgatacaa
    20041 gatcatacag cacttgtgta cttgggtgtt gtcctttgtt ttctatattt gttaaataac
    20101 gtgggtcaat ttcaatcatt gttcccactt gttcacgagt gagaccccgt ttcattcgag
    20161 cctctttaat cgctaaacca acggctctaa aatcgtattt atcttctttt ttacgcatac
    20221 taaatcacct ctatacattt tactgttcct atataaccat aaacaggtat agaaaaacat
    20281 gttatatagt ttatgagttc ctatagaaca ctgtggaatt tattaatgcg atgaatccaa
    20341 tttcaaatag aaaggtcgct gattttattt acctatacaa ctttataaaa tttagtcata
    20401 ctcctagaaa gtagataaaa ttggcgactt aatggaggga aaagtcgcca tgagaaagat
    20461 aatctgtcat ctagttctct taattaagtt cttatcaaaa aaacttatcc acctgtactg
    20521 gatagatatg accatcaatg tggttttgca gattacatag atagaaaaat caaagcatat
    20581 gaacagagaa aacaatctgt ttatgtgctt ttttggtcac ttaataaata tcttttagga
    20641 tttctcttca tttatgcaac aaatcccccg ttcgtgtcgg ggtttagtat gaagggagat
    20701 aaagaagcac atgccactcc ctttcacgac agaaaggggg tgaaaagcat gaaaccatct
    20761 tcttttcaag aaaccataga aaatcagttt gactatatct gcaaacaagt tattgaagat
    20821 gaacgaaaag attacttcaa acatctagac agactatcag caaaagaagt ctcgttttct
    20881 gatatagata actaccttgt caactctttt tctactgttg atacgtacgt cactgatttc
    20941 caactattta aattatttgg attaagaatc aatgttgaaa gtgatttatt aagtgaagcc
    21001 ctgcgtcatc tttcagagaa aaaacggaac attattttac ttcattactt tatggatatg
    21061 agtgatgtgg agattgcgga gttactaaat ttgaatcgtt cgactgttta tcgtcatcga
    21121 ataagtggac tagccatgat taaagaattc atgaaggagt gtaaagaatg aaaacagctt
    21181 atccaagggt tcccttcccc ctcattgtaa aggcgacaga tggcgacgtg gaagcaatta
    21241 atcagattgt taaacattat cgagggtata cctcaaaacg ttcattacgt cgcatgacag
    21301 acgaatatgg aaattctcat atggttattg atgaaaccct acgtggcaga atggaaacga
    21361 aactcatctc taagatttta tcgttcgaaa ttaaatagtc ccattttttc gtggaagcgt
    21421 gtcgaatcgt atccacgctt cccgatttgg gaggtttgtt attccatcaa agcatattgt
    21481 gtgttcagta ggttttgata ggctaacaag ccaaacgttc tttgacaacc aaataaaggc
    21541 aatcgaatac gtttcggtaa gaaaagagct gatgaactaa cacgccatga cctacaaaat
    21601 tagtgagcga ttcatgttag taattcgaaa gtaatcttta gcaggattgc tgccatgact
    21661 ttcttatcgt gataatgata ctcccgtata gccatagtcc gagcgttaga agcgtcacag
    21721 gcaatgggta cggctacatg agaaccatgc agaagtgaaa ttcctgtgag ctttgctaga
    21781 gctgttcgat tgctaagata ttcttctctt gtcagtgtat tattataaaa ctatttgata
    21841 taattatagg tgaatattgg agttttccta gcctataatt gggaaaggag taataatgat
    21901 aacggtcaca aaaaaggatt tagttgagct aggatatgga acttcttttt cagcagacat
    21961 cataaaaaaa gcaaaagaat tgatgattac aaaagggcat atctactatc aatctcgaaa
    22021 gctagataga gtccctcgtg aagctgttga agaaattcta ggaattcggt tttcaaatga
    22081 aaacaggtct gagtagtttt tgcacagtaa ggagtaaaaa ctatggttaa agacccaata
    22141 aaaaaagcaa agaatggaac ctattatttt cgtgcaaatc taggttatga ttctaaaggg
    22201 aaaaaaattc aaaaatatcg tagtggattc actactaaaa aagaagcaag agaagaatat
    22261 tcgaaactgc tattgatgaa accagaagaa ttaagtgaga ataaggataa aatgacgttt
    22321 aaacattata tctttgaaat tttccttcca tggtataaga cacaggtaaa gctcagaaca
    22381 tatgaaaatc gcttaccaac tattaagaag catttctctt attttgataa aatggctgtt
    22441 tccgatattg agcctattga tgtgcaaaat tggcaactaa aattatcaaa gaagtgtaag
    22501 tcttcttatg taagagctgt tcaaggacta ttttcagttg ctatggatcg agcaattgtc
    22561 cttgggataa ccaccacaaa tccatctaag attattggca atgtgaaaaa gcaaaagtct
    22621 aaaattgaat tttggacaaa agaagaattt gaaaaagtaa tctctctaat ttacaaagaa
    22681 gattactatc aacacttttt atttatctct ctgtggtttt tatttatgac tggaatgcga
    22741 attggtgaag ctactgccat tcaatgggaa gatattgatt ttgactcagg agtactaacc
    22801 atcaacaaaa cactctatta caagaatcaa tcgaattata gatttgttga accaaaaaca
    22861 aaggcgagcg ttcgtcatat tgtattagat aaatgcacat taacttattt atccgaatgg
    22921 aaggaagtac aacaaaatct aattcagaca gactttgtaa tgagctataa cggtattcct
    22981 acccaaaaac atacactatc atatgctatt gaacgctatg ctaagctggc tggtatccat
    23041 cgaatcagaa tccatgggtt acggcattct catgcttcct tgttaatcag tatgggagaa
    23101 aatccactta tcattaaaga tcgtctagga cacgaagata ttgagacgac tttaggaacg
    23161 tatggacatc tttatcctaa cagtaatttc gaggtagcta acaaattaaa tggagccatt
    23221 tcctttaaag aagcaaaaca aaattatgat acttcaccaa agaatcaatt tactgtagac
    23281 tacttgcgaa acaaagaaat gaaaaaagtg caataaaagt gcagtaaaaa gataagaaga
    23341 gccagaaacc cttggtataa gaggatttct agctctccta caactattcc cactccactg
    23401 ttgctggtgg tttagaagtg atgtcataca cgatacggtt gacgtgggct acttcgttta
    23461 cgatacgtac agagattttt tgcaagactt cccatgggat acgggcaaag tcagcggtca
    23521 ttccgtcgat tgaagttaca gcacggatac cgactgtgta gtcgtaagta cgtccgtcac
    23581 ccataacacc gacagagcgg atccctggaa gaacagtgaa atattgccag atatcacggt
    23641 caagtccagc agctgcgatt tcttcacgca agatagcatc agattcgcgt acgatttcta
    23701 gcttgtcttc agtgatttcg ccaagaacac gaataccaag accaggacca gggaatggct
    23761 gacgccaaac gattgaatca ggcatgccta actgagtccc taattcacgg acttcgtctt
    23821 tgaataatgt gtttaatggt tcgatcaatt caaattgcat atcttctggc agtccaccta
    23881 cattatggtg agatttgatc gtctgtgctg tttctgtacc agattcgata acgtcagtat
    23941 acaacgttcc ttgtgctagg aaagagacgc cttcttcacc agcaagtttt gttgcttcgt
    24001 catcaaaaac atatacgaat tcattcccaa tgattttacg tttttgttct gggtcagata
    24061 cacctttcaa cttgtctaag aagcgatctc ttgcatcgac tttgatgatg ttcaagccga
    24121 attttccgcc tagactttcc attacttgtt ccgcttcgcc tttgcgtagc agaccgtggt
    24181 caacaaagat agatgtcaat tggtcaccga tcgctttttg taaaagaacc ccaacgacac
    24241 tagaatcaac gccacctgaa agaccaagca agactttttt gtcaccaact tgttcacgga
    24301 ttttttctac ttgcatatcg ataaagttat ccattgtcca gttaccttca cagtgacaaa
    24361 catcaaaggc aaagtggcgc agcaattcga ttccgtattc ggaatgacgt acttctgcat
    24421 ggaattggat accataaaaa ttacgtgttg tgtcttggat agaagcaatc ggacaatctt
    24481 tgcttgttcc gactgtttca aagccttcag gaactttagt taccaagtca ccatggctca
    24541 tccatactgt ttgttgtttt ggtgtttctt taaacaagac agcttgatcg cttgttactt
    24601 ctaacaaggc tttcccatat tcgcggtttt tagcaggctc tacggaaccg ccaagtttgt
    24661 acgtgatcaa ttgcatacca tagcaaatcc ctaatacagg aatacctaaa ttgaagatct
    24721 caggatcgat cccaaatgca ttctcatcat atacactgtt tggtccgcca gaaagaatga
    24781 ttccctttgg attcatcgct tttacttctt ctgcagtaat gcggtggctc aataattcgg
    24841 aaaagactcc aagatcacga atacgtcttg tgatcaattg gttatactga ctaccgtagt
    24901 ctaatacgat aatcttttca acgttcgtca tatcggcaac gtttgtcaaa tttattaccc
    24961 ctatcccttt aaattttatc tcaaaatagt caaactattt taaaatgcta atattttcgc
    25021 atgaaaatcg aatcgatgat atgattttca gttttatgga gaattatatc ggctcttcct
    25081 cgtgtaggca agatatattc ctgtaaattt ttcaaattca catttttcca tacatctttt
    25141 gccattttaa aggcttcttc acgatctccg atagcaaaac gataataata attctctgga
    25201 ttttggaaag ccgtatccag taacgcacca aaacgatcca aataccattt ttcgatcagc
    25261 acgggatcgg catcgacata gacagaaaaa tcaaaaaagt cgctgacgta gatttgctga
    25321 ttcgcaggta attgaagcgt attgatcccc tcaacgatca agatatcagg ctgctggatc
    25381 aattcgtact tgtctggaat gatatcatac acatcatgtg agtaaacagg tacttttatc
    25441 tcttcctgac cagattttac acggttcaag aaatcgatca gcatctccat atcataactt
    25501 tcaggaaatc ctttacggtc catgattcca cgctctttta atatacgatt tggataaaga
    25561 aatccgtcag tcgtgatcag ctgaacgttt cttcgtttga aggtacgaga caaaatcgtc
    25621 tgcagcagtc tggctgttgt actttttcca acggctacac ttccggcgat cccgataata
    25681 aaaggcggta cttctacgta tttatgaaga aataaacctt tactcaagaa caaagattca
    25741 aattctttca tataaagata aatcagatga gcaagaggta catagatatc tcgaacatcc
    25801 tgcatggaaa tgcggtcatt caaacttttg atattatcta gttcatcttg tgtcaaaggt
    25861 gcttgcccat tacgataaaa ttctcgccat tcttctcttg ccacacggta gtagttcatt
    25921 ggatcattca tattagcctc ctgcgttctc ccatcaatgg aactatttta acacaaaata
    25981 aacgcaaacc ccaccactta agagagattg ccgatctttt tgtataatct ttataataga
    26041 aagaaacgga gtcttattct tgacttgcgc acggatacat aaaaggatag actgttgtca
    26101 tgaaagaaat gagaaggatg gaagacatga caaatcatta ctattctgaa cgcccagata
    26161 cggcgcacga ttttgaacaa tgggcttttg agttaaaagg cagaaccttt caatttgtaa
    26221 cagattctgg tgtattttca cgtgaaacag tggattatgg ttcacgtgtc ctgattgatg
    26281 ctttcgattg ggaagagtta cctgatgaag gaaagatctt agatgtggga tgcgggtatg
    26341 gaccgattgg gttagctttg gcatttgcga cacagcggtt tgttgaaatg gtcgacatta
    26401 atgaacgagc ggtaagtcta gcgcaaggaa acgcaaagcg taaccaaatc gaacatgttg
    26461 atatccatca atcgaatatc tatgaagcag ttcatgaaga aacctatgca gctatcgtaa
    26521 gcaatccgcc gatccgtgct ggaaaaaaag ttgtacatga aattttgaca ggcgcatatc
    26581 ctcggttgaa gaagggcggt acactgacaa ttgtgatcca gaagaaacaa ggagcgccga
    26641 gtgcgcaaaa gaaaatggaa gaaacatttg gtaatgcaga aatcgtcaca aaagataaag
    26701 gatattatat catcaaaagt gtgaaagaat aactagctga aaaatagtgg agcgacgaaa
    26761 gagtggaact gggcgtgcat gtacgggtac gtatcagttc tttttttgtt gagaagtttt
    26821 taaaaacaaa gaactatttt ttcttcaaaa agaaatagta ggaacagagt cgttgttttt
    26881 tacgataaaa taaataagat acagaacttg tatagaaggc tgccaagtcg gtagataaag
    26941 gaggttttta tcaaaaaaca tactttcctt ttttctctat tcttttcttg ggggttttgg
    27001 tacactgata gggaatttag atagaaggga gccaatagga tgaagctgac catcagagat
    27061 atcgcagaaa tggcaggcgt atcagtaaca actgtctcac agattttgaa taacaaagga
    27121 agcagattta gtgataagac aagaaaaaaa gtgttgtcga tcgtgaatga acatcattat
    27181 aagcccgatt atttcgcttc aaatgtgatc aatcgacatt caaagaccat cggaatgatc
    27241 gtccctgatg tcactgattt cttcttctcc aaactgatcg aaggtgtaga gagctatttg
    27301 aatcctttgg gctatgtgat catgttatgc aattctcgtc atagccaaga aaatgagatc
    27361 aagtatttac aagagctttc ccaccgctca gtggacggaa tcttgctggc cacaccaaat
    27421 attttacctg agaaatatgc attagacagc ggtttttatc aaaaaatgcc aatcatttta
    27481 atcgatcgcg gattgaatcg tcgggataac ggacggctta tcgtaaagga atatgagggc
    27541 gcctatcaag ctgtttcgct gtttattgaa aatggccata cgaagatcgg catgctgaag
    27601 gaaaccactg gatactatca attagaagaa cgtttcaacg gatatcgtca tgcgctgaaa
    27661 gatcatggcc tgccttttaa tgggaaattt gttgaacatg gagagttgac tgtccaaggt
    27721 ggatatactg catcaaaaaa attactcaaa cataaagata tcacggctat tttttgcggg
    27781 aatgatgcca tggcgatcgg atgctatcag gcgatcgatg aattgggcaa aaaaatccca
    27841 gaagatatct cagtcatagg attcgatgga ttaaagctgt cagaatacat gattccgcaa
    27901 ttgacgacag tgcagcagcc aagttttgat attggatttt atgctgcacg tttcctgatc
    27961 gatacgatcg aatttcctca aagaaaagtt ccgaacaaag tgttcgaaac aaaattaatt
    28021 attcgtgaga gcgtaaaagt attgacaaag ggctaactcg gcggtatatt gcttatgtaa
    28081 gcatttacaa aggctagtta gtgctttttt tatttttagt aaaccagttt actaaagtgg
    28141 tttacctttc ctaaaaataa tgtataatac cgaatgaaga caaggaggat caggcaatgc
    28201 gaatggtgga tctgattgaa aaaaagaggg atggaaacga gctgtcgaaa gaggaaatcg
    28261 agtacatcgt cacgaactat acgaatggaa agattccaga ttatcaagtc agtgcattat
    28321 tgatggcaat tttttatcaa gacatgacaa atgaagaaat cacgaactta acattggcga
    28381 ttgctaattc tggtgatgtc atcgatttga gttctcttga aggaataaag gtagataagc
    28441 attcgacagg tggcgttggt gacacgacca cattgatttt agccccccta gtagctagtg
    28501 tcggagtgac agtggctaaa atgtctggac gcggtctagg ttacacaggt ggtacactag
    28561 acaagctaga agcaattccc ggatttcaga tcgagctttc ggatgaagaa tttgtacgca
    28621 ttgttaatga aagtaaagta gcggtaattg gtcaatcagg aaacttggca ccggcggaca
    28681 aaaaattgta tgccttgcgt gacgtcacag cgaccgttga ttctcttccg ctgattgcca
    28741 gttcgattat gagtaaaaaa atcgcggcag gagcagatgc aatcgttttg gatgtgacca
    28801 ctggagacgg tgctttcatg aagaacatcg aagatgcaag acgtctggca aagaccatga
    28861 cgagcattgg gaaactggct aaccgagaaa cagtggcggt tatctcagat atgtcagaac
    28921 ctcttggcga agctatcgga aatagtctag aagttgtcga ggcaatcgaa acattacaag
    28981 gaaatggtcc agaagatcta gtagaaatgt gctacgcctt aggaagccaa atggttgttc
    29041 ttgctggcaa agctaagaca accgatgaag caagaacgtt gcttcaagaa gctctggaat
    29101 caggtaaagc gttagcaaaa ttcaaagaaa tgatccaaaa tcaaggggga gacccagcga
    29161 tcgtggaaca tccagaacga attttgactg cacgctacac aatggagctt cctgctaaac
    29221 aaagcggagt tgtgtcaaaa atcgtggcaa atgaattagg aattgctgca atgatgctag
    29281 gtgctgggcg taaaaccaaa gaagaagata ttgatcacgc tgttggattg aaactgcata
    29341 aaaaaattgg tgacactgtg acaaaaggtg agtcactatt gacgatctat agtaacgacg
    29401 aagaaatcac ttctgtcatt gaattattat ataaaaatat tgaaattggt gaatcggcaa
    29461 tgaagccaac gttgattcat gacatcatca cagaataaag gaaaaaagcg gactttaccg
    29521 ttttaaagaa aaggggaaaa gaaatggaac taaaccgaat gatcgaccat actattttga
    29581 aagcagacgc atctaaagaa gctgtgatgc aaattatcga agaagcaaaa aaataccatt
    29641 tttattctgt ctgcatcaat ccagcatggg ttgcgctagc gaaagaacaa ttacaaggtg
    29701 agccagtagc cgtatgtaca gtaatcgggt ttcctctagg agcaaacaca cccgagacaa
    29761 aagcatttga aacaacagat gccatcaata acggtgcaga tgaagtcgat atggtcatca
    29821 atatcggtgc tttgaaatca aaggatgatc aacaagtcca aaaagatatc gaagcagttg
    29881 ttgaagcagc aaaagatcgt gcgcttgtca aagttattat tgaaacatcg ttattagata
    29941 gagaagaaat catccgagct tgcgaaatag caaaagctgc aggcgctgat tttgtcaaaa
    30001 catcaactgg tttctcaaca ggcggagcaa aggtagagga tgtccgtttg atgcgtgaaa
    30061 ctgtaggacc agaaatgggt gtcaaagctt ccggcggtat ccataacgaa gaagaagcaa
    30121 tggcaatgat cgaagctggt gctacaagaa tcggaacaag cgcaggagta gcaatcgtat
    30181 ctggttcaac tggtgaaggc tattaatttt ttcaaaggag aagctcatgg cagaactcaa
    30241 acaagaatgg atcaacacag ctattgaagc gttagataaa gcctatgttc cttattccca
    30301 ttttccggta ggcgcttgtc tcgtaacgga atcaggcaag atttatcaag gcatcaacat
    30361 tgagaatgca tcatttggat taacgaattg tgccgagcgc acagcttttt tcaaagcagt
    30421 atctgaagga gaaaaaacat ttacgcatct agtcgtagcg gggcacaccc ccgatcctat
    30481 ctctccttgt ggtgcatgtc gacaagtaat ggcagaattc tgttcccctg atatgccggt
    30541 tacgctagta ggagacaatg gtgtgacgaa ggcaacgacc gttcgtgcac tgctgcctta
    30601 tgcatttact gaaaaagatc tataactcat gttcattcag tcagctgact gttgaaaaat
    30661 aaaacaatta ttttaggagg ctttacagaa atgaaaaaag caaaattatt tggtttaggt
    30721 gctgtcgcac tagcagcagg gttattttta ggtgcatgcg gcaacaacgg ctctactgat
    30781 tcatccggtg gcaaaagctc atcagacaca acaactgcag cattgatcac agacacaggt
    30841 ggtgtagacg accgttcatt caaccagtca gcttgggaag gactagaaaa atgggggaaa
    30901 gatcaaggac tttcacgagg aaatgatggc ttccaatatt tccaatcttc aaatgaatca
    30961 gattacatcc caaatattga ccaagcattg aatgctggat tcaaaacgat ttttggtatt
    31021 ggttataaac taaaaccagc gatccaagaa caagcaacaa acaatacagg tacgaacttt
    31081 gttattatcg atgatgtcat cgatggatta gataacgttg tatctgcaac tttcaaagac
    31141 aacgaagctt cttatcttgc aggtgttgca gcagcttata cgactgaaac aaacgtagtc
    31201 ggctttatcg gcggtgtaaa aggtgaagta atcgatcgtt ttgatgctgg attcaaagcc
    31261 ggtgttgatg caggtgcgaa agaattagga aaagaaatca aagtattgaa tcaatatgct
    31321 ggtgacttct ctgctccaga taaaggacgt tcaattgctc aaggtatgta tgcacaaaac
    31381 gcagatatca tcttccatgc ttcaggcggt acaggtaacg gtgtattcca agaagcaaaa
    31441 tcattgaacg aatcaggcga caaaaaagtt tgggttatcg gtgttgaccg cgaccaatca
    31501 gacgaaggcg aatacacatt aaacggcgaa aagaaaaact tcacattgac atctacactg
    31561 aaagcagttg gaacagtagt agaagatttg gcacaaaaat cagcggacgg taaattccct
    31621 ggcggcgaac acactgttta tggattgaaa gaagacggtg ttggtctaac agaaggacaa
    31681 ctttctgacg aagcgaaaaa agcagttgat gaagcaaaag aaaaaatcat ttcaggtgac
    31741 gtaaaagttc cagaaactcc tgaagaaaac taaaaccatt tctatagcct tgttagacac
    31801 atgtttgaca aggctatggt ttgattagaa caagaattgt tcatttatcc aagctgcctt
    31861 gtgacagttt ggataaggga acaactccct gaagtaatgt ttgtttcaaa agaagaacgc
    31921 tcactaagcg tgaatcttag tcaatcgttg ccctagatag aaatcgttag gatgtgaaaa
    31981 aaaggtggtt caagaaaata ttgtcataga aatgcagaat attaccaaag aatttggaac
    32041 attcaaggca aatgacaaca tcaatttaca agtaaaggca ggagaaattc atgcgttgct
    32101 tggagaaaac ggtgctggca aatctacatt gatgaacgtg ctttccggat tattagagcc
    32161 gacatcaggg aaaattttga tgcgtgggaa agaagtaaag atcacaagtc cgaccaaagc
    32221 caaccaatta gggattggga tggtccatca gcactttatg cttgttgatg cctttactgt
    32281 aacagaaaac atcgtgttgg gaagcgagcc tagtcgcgca gggatgcttg accataaaaa
    32341 agcacgaaaa gagatccaaa aagtttctga acagtatgga ttgtcagtca acccagatgc
    32401 ttacgttcgt gatatttcag ttggaatgga acaacgggta gaaattttaa aaacacttta
    32461 ccgaggagca gatgtactga tttttgatga gccgacagct gtattgaccc ctcaagaaat
    32521 tgatgaatta atcgtgatca tgaaggaatt agtcaaagaa ggcaagtcga tcattttgat
    32581 tacgcataag ttagatgaaa tcaaagcagt agctgaccgc tgtacagtta tccgccgtgg
    32641 aaaaggaatc ggtacagtca acgttaaaga cgtaacctca caacaattag ccgatatgat
    32701 ggttggaaga acggtttcat tcaaaacgat gaaaaaagaa gcgaagcctc aagaagtcgt
    32761 tttgtctatt gaaaatctag tggtgaaaga aaatcgtgga ttagaagccg tgaaaaactt
    32821 gaacttagag gttcgtgctg gcgaagtact tggtatcgct ggaatcgatg gaaacgggca
    32881 gtcggagttg atccaagctt tgactggttt gcgaaaggca gaaagcggac atatcaagct
    32941 aaaaggggaa gacatcacta ataaaaaacc ccgaaaaatc actgaacatg gtgtaggaca
    33001 cgtgccagaa gaccgtcata aatacgggtt agtcctagat atgacattat ctgaaaatat
    33061 cgcactacaa acctatcatc aaaaacctta cagtaaaaac ggcatgctga attattcagt
    33121 aatgaatgaa catgccagag aattgatcga agaatatgat gttcgaacaa cgaatgaact
    33181 tgttcccgca aaagctttat caggcggaaa tcagcaaaaa gcaattatcg ctcgtgaagt
    33241 cgaccgagat cctgatctgt tgattgttgc aaatccaact cgtggactgg atgtaggtgc
    33301 gatcgaattt attcataaac gtctgatcga acaaagggac aaatacaaag cagtgttact
    33361 gatcagtttc gaattagaag aaattttaaa tgtttcggat cgtattgctg ttatccatga
    33421 aggagaaatt gtcggtatcg ttgatccgaa agaaacatct gaaaatgaat taggtctatt
    33481 gatggcgggc tataccttag aagaagcacg aaaagagtta gaaaaggggg ctggcgcaca
    33541 tgaatgatcg cagcgaaaaa ttccgtaatt tattagtgcc tgtactttca gttgttttag
    33601 gattgatctt aggcgcgata ttgatggctg cttttggttt tcagcccatc caaggttatt
    33661 cttctatgct agatgcagcc cttggttcgc aaagagggat aggtgaaacg ttaaggcaag
    33721 caacgccgtt gatttttaca gcattaggtt tttctgtagc taactcagct gggtttttca
    33781 atattgggct ttctggacaa gcactttgcg ggtggatcgt cagtgtctgg gtggcacttg
    33841 cgtttccaga tctgccaaaa gttgttttgc ttccgttatg cgtgatctta ggtgccttag
    33901 ctggtgcgat ggcagcggct gttccaggtt tacttcgagc attttttgga acaagtgaag
    33961 tgatcgtaac aatcatgatg aactatatct tattatacgt aagcacattt gctttacatg
    34021 aagtgatgcc tgaaagctat cgctcaagtt tggactcttc caatatgatc agtcaaaatg
    34081 cctcactgcg gttaccatgg ttaacacaag cttttggcgg ctctcgcgta aactcaggat
    34141 tatttttagc aatcatcgcg cttattttcg tttggctgct gatgaagaaa acgactttag
    34201 gatttgaaat ccgttctgtt ggattgaatc cttttgcttc tgagtatgca ggaatgagta
    34261 gtaaacgtac gatcgttttg tcgatggtca tttctggcgc tttggcaggg cttggcggtg
    34321 tggttgaagg attaggaacg tatcaaaact tctttgttca aacgacttcg ctatctattg
    34381 gatttgacgg aatggctgtt tccttgcttg gttctggatc agcaatcggt attcttttat
    34441 cagcgatctt gttcagtatt ttgaagatcg gtggacttgg catgcagaca ggagcaggtg
    34501 ttccgtttga gatcgtcaat gtatcgattg ctttgattat cttctttgtc gggatcaact
    34561 tcctgattcg tttcgtcatg gctaaagtct tccaagggaa aaaacaagaa gagatcgttg
    34621 cgacaataga atcgaaacca acgaatcaaa accatgaagg gggcgaacta taatgtcgac
    34681 tatcgaatta attgccttga tccttacttc tacactggtt tattcaacac cgctaattct
    34741 aacgtcacta ggcggaactt tttctgaacg aagcgggatc gtaaacgtag ggctagaggg
    34801 aatcatggtc atgggtgctt ttagttccat cgttttcaac ttgacgatga gtccatcact
    34861 tggttctatg acaccatgga tcgctatttt agtgggtggc gtcgtaggta tcctattttc
    34921 tctgattcac gcagtagcaa cagtcagttt gcgtgctgac catattatca gcgggacagt
    34981 aattaatctg atggcacctg ctttaggtgt tttcttgatc aaagcatggt atggtaaagg
    35041 tcaaactgat aacatcacgc agaatctggg gtatttttct ttcccaggat tagcagatat
    35101 tccggtcatt ggaccaattt tctttaaaaa tacattctta cctgcttatt gtgctatttt
    35161 gattgctgtc ctagcttggg tgatcatctt caagacgaaa ttcggcttgc gcctccgttc
    35221 agttggtgaa aatcctcaag ctgccgacac attaggtatc aatgtatatg ctatgcgtta
    35281 ttcaggcgta ctgatttctg gcttgttagg cggtatgggc ggtgctgttt ttgctcagtc
    35341 gatttccggt aacttttcag ctggtacgat cgttggacaa gggttcatct ccatggctgc
    35401 tatgatcttt ggtaagtgga atcctttagg ggcaatgggt gcctcattgt tctttggttt
    35461 tgctcaaagc ttgagtatca ttggcgcaca attaccagtt atttcttcga ttccgccagt
    35521 attgctgcaa attgctccat atttgctgac aatcattgtg ctggttgttt tcttagggaa
    35581 agcttctggt ccaaaagcaa acgggaagaa ctatatcaaa tcaaaataat cttttagcga
    35641 ataaaaaggg tgggttacgg gaagtgtttt tcgtctcgcc ctttttgttt taaaggcaat
    35701 aaaaataaaa agaaagaagg aataatcatg tttaaacgcg tacatctgat cgtcatggat
    35761 tcagtaggaa ttggagaagc accagatgcc gataagttcg gtgatgttgg ttctgataca
    35821 ttaggacaca ttgcgaaaga agcaggattg aacgttccac atttagagca gcttggttta
    35881 ggaacgatcc gtcctcttga aggtgtggaa tcagtgaaag atcatcaagg ctatgcgacg
    35941 aaattagaag aagtttctgt agggaaagat acgatgacag gccactggga aatcatggga
    36001 ttgaatatca aaactccatt tcgtgtgttt cctaatggat tccctgatga tctgatcaaa
    36061 caaatcgaag aattttctgg ccgtaaagtc gtatgtaaca aaccttatag cggaacagct
    36121 gttattgacg attacggtga acaccaaatg aaaacaggcg atttaattgt atatacttct
    36181 gccgatcctg tccttcagat cgcggcacat gaagaaatca ttcctttaga agaattatat
    36241 cggatttgcc aattcgttcg tgatataaca aaagatgatc cgtatatgat tggccgaatc
    36301 attgcacgtc cgtacgtggg tgaaccagga gacttcacac gaacaagcaa tcgccatgat
    36361 tatgcgttga atccattcgg acacacagtc cttgattctt tgaaagaagc aggaaaggac
    36421 gtgattgctg ttggaaagat caacgatatt ttcaacggac aaggaatcac tgaatctgtc
    36481 cgcacgaaaa gcaatatgga tggcgttgac cagctgctga aagttatgaa aaacgatttt
    36541 tcaggaatca gctttaccaa tttagtagat tttgatgcat tatacggtca tcgtcgggat
    36601 gtagtcggct atgcgcatgc gattgaagaa tttgacttac gaattccaga attgcttgaa
    36661 gcgatggaag aagacgactt gctaatgatt acagccgatc acggaaacga tccgacgttt
    36721 actggtaccg atcatacgcg agaatatgta ccgttattgg tttacagtaa aaaaatgaaa
    36781 gaacaagggg cattgcctca aggatattat tcagatattt cagcaacgat tgctgagaac
    36841 tttagtgttt cttcgactga aaatggcgaa agcttcttga aattattaaa ataatctttt
    36901 taaataggag aaagcaaatg accaagttaa gcgagatgtt acaagaaacg acccagttta
    36961 tccttgataa aggagtaaaa gaaattgatt ttggtttgat tctaggttca ggattaggtg
    37021 aattagcaga tgaaatcgaa gatgctgtaa tgatttctta tgatgagatc ccgttctttc
    37081 ctgtttcgac agtagttggg catgcaggaa agctagttta tgggtctctg gcaggcaaga
    37141 aagtgttagc catgcaaggg agattccact actatgaagg tcattccatg caaacagtga
    37201 cctatccagt acgtgtgatg gccggactag gggcccattc agtcattgtg actaatgcat
    37261 gtggtggtgt caacgaatca tttgtaccag gagatttgat gctgattacc gatcatatca
    37321 attttactgg tgaaaatcct ttgatcggtc cgaatgaaga tgcaatgggc cctagatttc
    37381 cagatatgag tcaagcttat acgcctgact atcaagcaac agcaaaagca gcagcagaaa
    37441 aactaggttt ggatcttaaa gaaggcgtat atatgggcta ttctggacca acttatgaaa
    37501 cgcctgcgga gatccgaatg tctcggacaa taggagccga tgcagtaggg atgtcgactg
    37561 ttccagaagt catcgtagca gctcacagcg gattaaaagt gttaggaatc tcttgtatta
    37621 ccaacttagc agcaggtatg caagcgaatc tgagccatga agaagttgta gaaacaactc
    37681 agagagtcaa acaatctttt aaagcattgg ttaaagaaac attgacatta ttgtaatagg
    37741 aggaacaata aaatgagtgt gcatattgaa gcgaaagaag gcgagatcgc cgataagatt
    37801 ttacttccgg gagatccttt gcgagctaaa tatattgcgg agactttttt ggaagatcca
    37861 gtttgttaca atcaggtacg cggtatgtta ggctttaccg gcaaatataa aggagaacgt
    37921 atttctgttc aaggaacagg aatgggaatg ccatcagcca ccatctatgc ccatgaattg
    37981 atccagtcgt atggtgtaaa gaaattgatt cgagtaggta catgcggtgc attgtcaaaa
    38041 gacgtccatg ttcgtgattt agtactagca caaggtgcag cgaccagttc ttcgatgatc
    38101 gaaaaaaatt tccaagcctt tcattttcct ccaatcagtg atttcaatct attactgaaa
    38161 gcatatgaga tagctaaaga aaagggctat acggttcatg taggaaatgt tctttcggaa
    38221 gattctttct acaaagatga tttgacagag acgttccaat tagctgaact tggcgtttta
    38281 ggagtggaaa tggaagcagc ggctctttat tacttaggcg cgaaatacca tgtgcagaca
    38341 ttaagtttga tgaccgttag cgaccatttg atcaccggtg aagaaacaac agctgcagaa
    38401 cgtcaatcta cgttcaatga aatgatcgaa gtaggattgg aaacagcgat agcagaataa
    38461 gaaggaaatg aaaaagctgc cctgcattaa gtaataagaa catccaagca aaaatagctc
    38521 ctcatgtttt tgacttggct ggacttatta ttgcaaatgt aagcttttga acaccgttta
    38581 ccaagaggtt gtgaatctgc cgtttaactc tgagtcacaa ggaacaattt caaaaaacag
    38641 cttttcatgt ttgatgaata ttgtgacttg tgaccgagaa gttggcagga gaaaaccgtt
    38701 tatacgaaaa aagggactgt gacaaatcgt gtcacagtcc cttttttata gtgtttttta
    38761 taggtagtat tttttagcaa ctgttaagtc atcatttaat tcataaacaa gtggttgacc
    38821 tgttggaatt tcaagatcca tgatgtcttc gtcagagata ccttcgatat gtttagctag
    38881 ggcgcgtaat gagttaccat gtgcagctac taaaacagtt ttgttgtcta gtaatgctgg
    38941 agcaatttca tcttgccaga atggcaaagc gcgttctaat gtcactttca agttttctcc
    39001 accaggaata tcgcgtttgt ctaacattgc gtaacgacgg tcatttgctg cagaaccttc
    39061 gtcagtttct tccattaatg gaggaagagt atcgtaagaa cgacgccaga tgtgcacttg
    39121 ttcgtcgccg tatttttcag ctgtttcttt tttgttcaaa ccttgtaatt taccatagtg
    39181 acgttcgttt aagcgccatg atttgatttg tggtacccaa agttgatctg aatattctaa
    39241 gatcaagtta catgttttga ttgcacgagt taaaacagaa gtgtaagcaa catcaaattc
    39301 gattccagct tctttgattt tgcgtccgcc ttcttttgct tcttcgattc cttctggtgc
    39361 tagatttaca tcagcccaac cagtaaattg gttcaatgca ttccattcac taagaccgtg
    39421 acgagaaaaa actaattttg gcatgtttag ctctcctttt aagataggtt tatatttctg
    39481 aaattttcag ttctgtttta ttgtacaatt ttttggaaga gatttccatc gaaaaattag
    39541 ataagattct atgagaaaac aagtagagaa tataaaaaaa cgaacagtgc ccacgcaact
    39601 gttcgttttt aggtattaga tgcataattg ctcgatttct ttttcaggta aattgatata
    39661 tttataacat gttccaactg aaacaccgca ttttgaagaa ataaattgga tcgtttcttt
    39721 tttttcataa tagagacgtc ggattttttt gatcgttttt gcatcgattt ttggtcttcc
    39781 gccgattttt ccttttttac gagcttcatc taatccaact aatgtacgtt ctttgatcaa
    39841 tgcgcactcc atgtttgcta ggttttccat taattggaaa tagatttttc ccatttcgcg
    39901 cgtatcgatt ccttcatcaa gactaatcaa gtgcagcccg cgatctttga ataattgcgt
    39961 gagttctgtt aattgccgcg tagatttgcc taatcgatga agttggcaaa tgactagtgc
    40021 atcgccttgt tgcatgtcag cgatcactga ctccaaatga acagcagtag aatcattttg
    40081 ttccacttca tatgtctcat ggaaaatttc ttgacaacca aagtcagata attgatcaac
    40141 ctgagttgtt aaatcaacgt catttatggt tgtgcgtgca taaccaataa ttttcatggt
    40201 gattcctttc tagcagtgat tttctaactg gatataacag taaaaatgta gcataaagta
    40261 cgtcaaaaag atagtttttt cgcaaatttt ccgctgtctt tttttattgg atgatcaagc
    40321 agtttttctt tatttacgac ggattgcgtc aagaatctca acgccttttt ctgagccgac
    40381 aactacttta gaaaccatgt ttaggaaaag cccatgttct actaccccaa caagttcatc
    40441 taaataggta gctaagcttt ctggctgttc gatgacttct agatgcagat caatgatgta
    40501 atgaccacca tctgtggtta agacttctcc gttttcgtct gtacgcaaga cgggttggaa
    40561 tcttttttct tcaaaaatat gaagtagctg ttgggagcca taagggatca cttcaactgg
    40621 gagtgggaac ttcccaagtt tatcgactaa tttagaagaa tcaacgatcc aaatcgtttc
    40681 ttttgagtac gtagcgacta ttttctcgaa aagcaaagca gcgccgccgc ctttgatccc
    40741 ttgaaaatca gcactgattt catcagcacc gtctattgtc aaatcgacta cggggacttc
    40801 atcaatactc tttaacggta ttcctaattt ttccgcttgt tcttttgtcg catttgaagt
    40861 agtaactcct gtgatacgta aaccttcttc acgcatacgg cgaccaatct cttctaccat
    40921 ataataggcg gttgaaccgg tcccgagacc tacgatcatc ccgtccttta cgaatttcgc
    40981 ggcttcaatg ccagctaatt gttttaaatt catctttgcg tccacctttt tttagtttga
    41041 gcatcgtgct ttcctcacta ttttagcgtg atttacggta aaaagatacc attctttttt
    41101 attccaataa agctttttca aaaaaaattg catctcttgt tgacatgttt tttcattcgt
    41161 gatattctaa caaaggtgct ttatctacag gtttctgtga agaaacgtgc tgactgttta
    41221 tcaaagcata tttgataagg atgcagacat tgcatttatt ttttatcaca aaaaaagaac
    41281 cacctggatg tgtggaacta gacgcaaatc gtgggaagga ggaaacaagg aatgcctaca
    41341 attaaccaat tagtacgtaa acctcgtaaa tcaaaggtgg aaaaatctaa ttcacctgct
    41401 ttgaacaaag gatataacag ctttaagaaa actcaaacta acgtaaactc acctcaaaaa
    41461 cgtggggtag caactcgtgt gggtacaatg acacctaaaa aaccgaactc agctttacgt
    41521 aaatatgcac gtgttcgttt gtcaaactta attgaagtta ctgcttatat cccagggatt
    41581 ggtcataact tacaagaaca cagtgttgta ttattacgcg gtggtcgtgt aaaagactta
    41641 ccaggggtac gttaccatat cgttcgcggt gctttagata ctgctggtgt taacgaccgt
    41701 aaacaaagcc gttctaaata cggaactaaa aaacctaaag cttaatttta attattaaac
    41761 aaatgaaact tgcttctact atatagaagt aattactatg aacaatattt cggaaggagg
    41821 agttacggat gccacgtaaa ggtcctgttg caaaacgtga tgttttacca gatcctattt
    41881 ataactcaaa attagtaact cgcttgatca accgtgtaat ggttgacgga aaacgcggga
    41941 ttgcagctaa cattatctat aatgcctttg acttgatcaa agaatctaca ggtaacgatc
    42001 cattagaagt gtttgaacaa gcaatgaaaa acgttcaacc tgttcttgaa gttaaagcac
    42061 gtcgtgttgg gggttcaaac taccaagtac cagttgaagt tcgccctgaa cgccgcgtta
    42121 ctctaggtct tcgttgggta gtaaactacg ctcgcttgcg cggtgaacac acaatggaag
    42181 aacgcttagc aaaagaaatc atggatgctg caaacaacac aggtgcttct gttaaaaaac
    42241 gtgaagacac tcataaaatg gctgatgcca accgtgcgtt tgctcactat cgctggtaag
    42301 attctctctg tttggtcggc ttttataagt cgacctcaga tagtgtcctt atttttagca
    42361 actagatagt aaagagagga gaaacaagga gaatgaccag agaattttct ttacaaaaca
    42421 ctcgtaatat cggtatcatg gcccacatcg atgctggtaa aacgactgct actgaacgta
    42481 tcttgtacta tactggtaaa atccataaaa ttggtgaaac tcatgaagga gcttcacaaa
    42541 tggactggat ggaacaagaa caagaacgtg gtattacgat cacttctgct gctactacag
    42601 cacaatggaa gggtcaccga atcaacatca tcgacacacc aggacacgta gacttcacag
    42661 ttgaagttga acgttcattg cgtgtacttg atggtgctgt aactgttctt gatgcacaat
    42721 caggtgttga acctcaaact gaaacagttt ggcgtcaagc aacaacttat ggtgtacctc
    42781 gtatcgtatt tgcaaacaaa atggacaaga tcggtgctga cttcttatat tcagtatcaa
    42841 ctttacatga ccgtttgcaa gcaaacgctc acccaatcca attaccaatt ggtgcggaag
    42901 acgacttcac aggaattatc gaccttgtaa caatgaaggc tgaaatgtat acaaacgatc
    42961 ttggaacaga gatcgaagaa acagaaattc ctgaagaata ccgcgaatta gctgaagaat
    43021 ggcgcgaaaa attggttgaa gctgtagctg aaactgatga agaacttact ttgaaatact
    43081 tagaaggtga agaaatcact gaagctgagt taaaagaagg tatccgccgc gcaacagtta
    43141 acgtagaatt ctacccagta ctatgtggtt cagcatttaa gaacaaaggg gttcaattac
    43201 tattggacgc tgttcttgat tacttgccat caccgttaga tatcccagct atcaaaggga
    43261 ttgaccctaa aactgatgaa gaagtagaac gtcctgcaga cgacagcgct cctttctcag
    43321 ctttagcatt taaagttatg actgacccat tcgtaggtcg tttaactttc ttccgtgtgt
    43381 actctggtgt tttacaatca ggttcatacg tacaaaacgc aacaaaaggc aaacgtgaac
    43441 gtgttggtcg tatcctacag atgcatgcca actctcgttc agaaatctct gaagtttacg
    43501 ctggagatat cgctgctgct gttggtttga aagacacaac tactggggac acactatgtg
    43561 atgaaaagag tctagttatt cttgaatcca tggaattccc agaaccagtt atccaagtcg
    43621 ctattgaacc taaatcaaaa gctgaccaag ataaaatggg tgtggcttta caaaaactat
    43681 ctgaagaaga tcctactttc cgtgctgaaa caaacgtgga aacaggcgaa acaatcatcg
    43741 ctggtatggg tgaacttcac ttagatatca tcgttgaccg tatgcgtcgt gaattcaaag
    43801 ttgaagctaa cgtaggtgcg cctcaagtat cttaccgtga aactttccgt gctggcactc
    43861 aagccgaagg taagtttgta cgtcagtctg gtggtaaagg tcaatacggt cacgtatgga
    43921 ttgaatttac accaaatgaa gaaggtgctg gtttcgaatt tgaaaatgcg atcgtcggtg
    43981 gggttgttcc tcgtgaatac attccagctg ttgaaactgg tttgaaagat gctatggaaa
    44041 atggtgtctt agctggctat ccattagtag atatcaaagc taaactttac gatggttcat
    44101 accatgatgt cgattcaaat gaaactgcct tccgtgttgc tgcttctatg gcattacgtg
    44161 cagcagctaa gaaagctaat ccagtaatcc ttgaaccaat catggctgta gaagttgtta
    44221 tccctgaaga ttatttaggt gatgttatgg gtcacgtaac tgctcgtcgt ggacgtgttg
    44281 aaggtatgga agctcgcgct ggcggccaac aagttgttcg tgcgatggtt ccacttgctg
    44341 aaatgtttgg ttatgctaca actcttcgtt ctgctactca aggtcgtggt acatttacaa
    44401 tgacatttga ccactacgaa gatgtaccaa aatcagtaca agaagaaatc attaagaaaa
    44461 acggcggaaa agctgaataa tttcttaaaa ccggatagtc ctgcaaagag attatccaaa
    44521 gagactatat ttttaatata gtttcgtgta aaataattat cgatgatatg ggcatgtggt
    44581 gtaccttgtg tacatcatat acctatcaga caaataaaaa atatattgta tttttaggag
    44641 gacattttaa aatggcaaaa gaaaaatttg accgttctaa accacacgtt aacattggta
    44701 ctatcggaca cgttgaccat ggtaaaacta cattaacagc tgcaatcaca actgtactat
    44761 ctaagaaaaa cggcggccaa gctatggctt acgatcaaat cgatggtgct ccagaagaac
    44821 gcgaacgcgg aatcacaatc tcaacagctc acgttgagta tgaaacagat acacgccact
    44881 acgctcacgt tgactgccca ggacacgcgg attacgttaa aaacatgatc actggtgctg
    44941 ctcaaatgga cggagctatc ttggtagttt ctgctgctga cggcccaatg cctcaaactc
    45001 gtgaacacat cctattgtct cgtcaagttg gtgttcctta catcgttgta ttcttgaaca
    45061 aagtagacat ggttgatgac gaagaattac tagaattagt tgaaatggaa gttcgtgacc
    45121 tattaacaga atacgaattc cctggtgacg atgttcctgt agttgctgga tcagctttga
    45181 aagctctaga aggcgacgct tcatacgaag aaaaaattct tgaattaatg gctgcagttg
    45241 acgaatacat cccaactcca gaacgtgaca acgacaaacc attcatgatg ccagttgaag
    45301 acgtgttctc aattactgga cgtggtactg ttgctacagg tcgtgttgaa cgtggacaag
    45361 ttcgcgttgg tgacgaagtt gaagttgttg gtattgctga agaaacttca aaaacaacag
    45421 ttactggtgt tgaaatgttc cgtaaattgt tagactacgc tgaagctgga gacaacattg
    45481 gtgctttact acgtggtgtt gcacgtgaag acatccaacg tggacaagtt ttagctaaac
    45541 caggtacaat cacacctcat acaaaattct ctgcagaagt atacgtgttg acaaaagaag
    45601 aaggtggacg tcatactcca ttcttcacta actaccgtcc acaattctac ttccgtacaa
    45661 ctgacgtaac aggtgttgtt gaattaccag aaggaactga aatggttatg cctggcgaca
    45721 acgtaacaat ggaagttgaa ttaatccacc caatcgctat cgaaaacggt actaaattct
    45781 caatccgtga aggcggacgt acagttggtg ccggtgtcgt tacagaaatc aaagcttaat
    45841 tcacttaagt tttaaagtaa agcaacattt attcggacgt aatcgctcgg aacgaaggtc
    45901 ttaggatctt tgttccgagc ttttttattt attgcaggat caggaaaaaa gacaggagta
    45961 ttataaatag aagaaacacg aaataaaaga gtcagtattt tctaaatgaa actttatgaa
    46021 aacaaaatgt tttttacaag aaaaagcctt gctttgtgag tttatatata ttataatgac
    46081 aaaggtgcgt ttactttagt gaattgcaca gacgtaatca gaatgatttc gtcggctaag
    46141 aaacgagagg ttgcgacacg cccggacgct ttgccacgaa cgagcgtgac ggaaattttc
    46201 gtggagctat gtctactttt caaaataggc gaaggaggga acaagatggc aaaacaaaaa
    46261 attcgtatcc gtttaaaagc gtatgaacac cgtattttag atcaatcagc ggataaaatc
    46321 gtggaaactg caaaaagaac tggagctagc gtgtctggtc caattccatt accaactgaa
    46381 cgcagtcttt acacaatcat ccgtgcgact cataaataca aagattcacg cgaacaattc
    46441 gaaatgcgta cacacaaacg tcttatcgac attgtgaacc caacaccaaa aactgttgat
    46501 gctttgatga agctagactt accatctggt gtaaacatcg aaatcaaatt ataaaactaa
    46561 aacaaaaata atggaggtgt actcatgacc aaaggaatct tagggaaaaa agtgggaatg
    46621 acacaaatct tcactgaatc tggtgaatta attccagtaa ctgttgtaga agctactcca
    46681 aacgtagtac tacaagtgaa aacaatggaa acggatggct acgaagctat tcaagttggt
    46741 taccaagata tgcgtgaagt tttatcaaac aaacctgcga aaggtcatgt tgcaaaagca
    46801 aacacggctc ctaagcgctt cattcgtgaa ttcaaaaatg ttgagctagg agaatatgaa
    46861 gtaggaaaag aaattacagt agatgttttc caagcaggag acattattga tgtaacaggt
    46921 actacgaaag gtaaaggatt ccaaggggtt atcaaacgcc acggacaaag ccgcggacca
    46981 atggctcacg gttctcgtta ccatcgtcgt cctgggtcaa tgggtccagt tgcacctaac
    47041 cgtgtattta aaaacaaacg tcttgctggc cgtatgggtg gcaaccgcgt aactatccaa
    47101 aacttggaag ttgtacgtgt cgatgcagaa agaaatgtta tcttgatcaa aggtaatgtt
    47161 cctggagcta aaaaatcttt aattactatc aaatcagctg tgaaagctaa ataattgtag
    47221 gaagaaagga ggaactaagg aatgccgaat gtagcattat tcaaacaaga tggaagccaa
    47281 aacggtgaaa tcacacttaa cgaagaaatc ttcggaattg aacctaatga aagtgttgtt
    47341 tacgatgcaa tcgtaatgca acgtgcgtca ttaagacaag gaacacacgc ggttaaaaac
    47401 cgtagcgctg ttcgcggcgg cggacgtaaa ccatggcgtc aaaaaggaac aggtcgtgca
    47461 cgtcaaggat ctatccgctc accacaatgg cgtggaggtg gcgtagtctt tggaccaaca
    47521 ccacgttctt acagctacaa acttcctaaa aaagttcgtc gtttagcaat gaaatctgta
    47581 ttgtcagaaa aagttgctga aaacaacttg gtagcaatcg aaggcttgaa cttcgacgca
    47641 ccaaaaacaa aagaattcaa acaagttctt gctaacttat ctatcgactc taaagtattg
    47701 gttgttctag aaaaaggtaa cgactttgct gctttatcag cacgtaactt accaaacgtt
    47761 tctgtagtaa cttctgataa tgtgagtgtg ctagacgttg tatcaaatac aaaagtgtta
    47821 gcaacacaaa ctgctcttac tcaaattgag gaggtgcttg cataatgaac ttactagacg
    47881 taatcaaacg cccagtgatc actgaaaaat caatgcttgc catggatgac aagaaataca
    47941 cttttgaagt ggacactcgc gcaaacaaaa ctttagtaaa acaagcggta gaagctgctt
    48001 ttgacgttaa agtcaaaaac gtgaacatcg tgaacgtacg tgcgaaattc aaacgtatgg
    48061 gcaaatatgc aggatataca aaaaaacgtc gcaaagcgat cgttacattg actgaagatt
    48121 caaaagaaat ccaattattc gaagctgctg aataagcact aatagataac tcattaaatt
    48181 aggagggaaa aacgtggcga ttaaaaagta caaacctacc tcaaatggtc gtcgtaacat
    48241 gacaggttct gattttgctg aaatcacaac atcaacacct gaaaaatcat tgttacaacc
    48301 attgaaaaac catgctggac gtaacaacaa cggccgcatc actgtacgtc atcaaggtgg
    48361 cggacacaaa cgccaatatc gcgtgatcga cttcaaacgt aacaaagata acgttgttgc
    48421 aactgttcaa acgatcgaat atgatccaaa ccgttcagct aacatcgcgt tagtacacta
    48481 tgaagatgga gtgaaagctt acatcttagc acctaaagga ctagaagtgg gtatgaaact
    48541 tgtttcaggt ccagaagcag atatcaaagt cggaaatgct cttccattag aaaacattcc
    48601 agttggtact gttatccaca acattgaaat gaaacctggt aaaggtggac aattgatccg
    48661 ttctgctggt acaagtgctc aagtacttgg taaagaaggc aaatacgtat tgatccgctt
    48721 gaactctggt gaagttcgta tgatcttagc tgcatgccgt gctacaatcg gttctgtagg
    48781 taacgaacaa cacgaattaa tcaacatcgg taaagctgga cgctctcgtt ggatgcgtaa
    48841 acgcccaact gtacgtggta gcgtaatgaa ccctaatgat cacccacacg gtggtggtga
    48901 aggtaaagct cctatcggac gtaaagcacc agtttctcct tggggtcaac cagctcttgg
    48961 ttacaaaact cgtaacaaaa aagctaaatc agacaaactt attgttcgtc gtcgcaaaaa
    49021 taaataatta ctttgaccat gtgtcgcaca ttttgagagg aggttcacca tgggtcgtag
    49081 tttaaagaaa ggaccttttg tcgatgagca tttaatgaaa aaagttgaag ctcaagcagg
    49141 ggttgaaaag aaaaaagtta tcaaaacttg gtcacgccgt tcaacagttt tcccaagttt
    49201 tgttggatat accatcgcag tttatgatgg acgtaaacat gtaccagttt acatccaaga
    49261 agacatggta ggacacaaat taggtgaatt tgcacctaca agaacttatc gtggacatgc
    49321 tgctgacgat aagaaaacga aacgctaatt tgaggggagg ataaatccaa atggcagaac
    49381 aaatcacatc agctaaagca actgcaaaaa cagttcgcac ttcacctcga aaagcccgtt
    49441 tagtaattga ccttatcaga ggtaaaagtg tcgcagatgc aatttcaatc ttgaaattca
    49501 caccaaacaa atctgctgga atcattgaaa aagtattgat gtcagcagtt gcgaatgcag
    49561 aaaataactt tgacttagat gttgaaaact tggtagtaag cgaagctttt gttaacgaag
    49621 gacctacaat gaaacgtttc cgtcctcgtg caaaaggatc agcttctcca atcaacaaac
    49681 gtacaagtca tatcacggta gtcgtatcag aaaaataagg agggataatc agtgggtcaa
    49741 aaagtacatc caattggaat gcgtgtaggc atcatccgcg actgggatgc taaatggtat
    49801 gctgaaaaag agtatgctga gttcttacac gaagatttaa gaatccgtaa atttatttca
    49861 actaaacttg ctgatgctgc tgtatcaaca gttgaaatcg aacgcgcaac aggtcgcgta
    49921 aacatttcaa ttcacacagc taaaccaggt atggttattg gtaaaggcgg ttctgaagtc
    49981 gaaagcctaa gaaaagaatt aaacaaatta actggtaaaa gaatccatat caatatcgtg
    50041 gaaatcaaaa aaccagatct agatgcaaaa ttagtaggtg aaggaatcgc acgtcaatta
    50101 gaaaaccgtg ttgctttccg tcgcgctcaa aaacaagcca tccaacgctc aatgcgtgct
    50161 ggcgctcaag ggatcaaaac tcaagtatct ggtcgtctaa acggagcaga tatcgctcgt
    50221 tcagaaggat actcagaagg aactgttcct cttcatactt tacgtgcgga cattgattac
    50281 gcatgggaag aagcagatac aacatacgga aaactaggag ttaaagtgtg gatctatcgt
    50341 ggagaaattc ttccaactaa aaaaaacact gagaaaggag ggaaataatc atgttagtac
    50401 ctaaacgtgt aaaacaccgt cgtgaattcc gcggaaaaat gcgcggggaa gctaaaggcg
    50461 gaaaagaagt agcattcggt gaatacggtt tgcaagctgt tgattcacat tggatcacaa
    50521 accgccaaat cgaagctgct cgtatcgcaa tgactcgtta catgaaacgt ggtgggaaag
    50581 tatggattaa aattttccct cacaaatctt atactgccaa agcaattggg gtacgtatgg
    50641 gttctggtaa aggggcacct gaaggatggg ttgcaccagt aaaacgtggt aaaatcatgt
    50701 ttgaaatcgc aggcgttcct gaagaagtag ctcgtgaagc gttacgtcta gcttctcaca
    50761 aattaccaat gaaaactaag atcgtaaaac gtgaggaaat gggtggtgaa tcgaatgaag
    50821 gttaaagaaa tcagagaatt aaccactgcc gaaatgcttg atcaagaaaa acaattaaaa
    50881 gaagaattgt ttaatcttag attccaatta gcaacaggtc aattagaaaa cactgcacgt
    50941 attaaagaag tacgtaaatc gattgcacgc atcaaaactg tgttgcgtga acaagctaag
    51001 taattatgga aggaggccat taagcgtatg actgaaggaa gaaatcaacg caaagtttat
    51061 caaggtcgcg tggtttcaga caaaatggat aaaacaatta ccgttgctgt cgaaacaaag
    51121 aaaaaccacc ctatctatgg caaacgcatg aactattcta agaaatacaa agtgcatgat
    51181 gaaaacaaca cagcaaaagt tggcgacatc gtgagaatta tggaaactcg tccattatca
    51241 gctacaaaac gtttccgttt gttggaagta gtcgaagaag cagttattat ctaataacac
    51301 gagattttcc tatatagatt gaatgcaaaa tctgaaagga ggatacacaa cgtgatccaa
    51361 gcagaaagtc gtttaaaagt cgctgataac tcaggcgcac gtgaaatttt gacgatcaaa
    51421 gtccttggtg gatctggtcg taaaactgct aacatcggtg atgtgatcgt tgctacggtt
    51481 aaacaagcaa cgccaggtgg ggttgtcaaa aaaggtgacg tcgttaaagc cgttatcgtt
    51541 cgaacaaaat caggagctcg tcgtacagac ggttcatata ttaaatttga tgaaaatgct
    51601 gcagtgatta ttcgtgatga taaaagccct cgtggaacac gtatctttgg gccagttgca
    51661 cgtgaactac gtgaaaacaa cttcatgaag atcgtttcac tagcaccaga agttttataa
    51721 tcttgagaca cttatcaaag gaggtgcgaa acagttatgt ttgttaaaaa aggcgataaa
    51781 gttaaagtta tcactggaaa agacaaaaac aaagaaggcg ttgtactagc agcgttcccg
    51841 aaaaaagata aagttattgt cgaaggtgta aatgtcgtta aaaaacacca aaaaccttct
    51901 caagctgctc ctcaaggtgg aattgttgag atggaagcgc caattcatgt ttctaacgtg
    51961 atggtggttg actctactgg tgtagctggc cgagttggct acaaagaagt agacggcaaa
    52021 aaagtccgtg tttctaaaaa aaccggtgaa gttttagata aataaaagtt taaggaagga
    52081 gggtcttact gaatgaaccg cctaaaagaa aaatatctta aagaagtaac tccatcattg
    52141 atggaaaaat ttaactatag ctctgttatg caaacaccta aagttgaaaa aatcgtcatc
    52201 aacatgggtg ttggtgatgc ggtatcaaac gctaaaaact tagataaagc agttgaagaa
    52261 ttaactttga tcactggtca aaaaccaatg attacaaaag ctaaaaaatc aatcgctgga
    52321 ttccgtttac gtgaaggaat gccaattggt gcaaaagtta cattgcgcgg agaaagaatg
    52381 tacgaattct tagacaaatt ggtttctgtt tcactaccac gtgtacgtga cttccatggt
    52441 gtaagtaaaa aagcctttga cggtcgtgga aactatactt taggtattaa agaacaattg
    52501 atcttcccag aagtagatta tgatttagta gataaagtac gtggtatgga catcgttatt
    52561 gtaacaacag caaactctga tgaagaatct cgtgagttgt tgacacaatt aggcatgcca
    52621 ttccaaaaat aattaaagga ggcgaactac gtggctaaaa aatcaatgat tgctaaaaac
    52681 aaacgtcctg caaaattttc aacacaagct tatactcgtt gcgaacgttg cggacgtcca
    52741 cattcagttt atcgtaagtt ccatctttgc cgtatttgct tccgcgaact tgcctataaa
    52801 ggtcaaattc ccggcgtgaa gaaagctagc tggtaaaaag taaattcgca taagaaggag
    52861 gtaaaagatc aatggtcatg acagatccaa ttgcagattt tctaactcgt atccgtaacg
    52921 caaacatggt taaacatgag tctttagaag tacctgcatc aaaaattaaa cgtgacatcg
    52981 ctgaaatctt gaaacgtgaa ggtttcgttc gtgatgttga atacatcgaa gatgacaaac
    53041 aaggcgtgat ccgtgttttc ctaaaatttg gtaaaaacgg cgaacgtgtt atcacaaact
    53101 taaaacgtat ctctaaacca ggtttgcgtg cttatgtcaa agctgacgaa gtaccaaaag
    53161 tattgaacgg tttaggtatc gcaatcattt cgacttctga aggtgttgtc actgataaag
    53221 aagctcgtga aaaaaatatc ggcggcgaag ttatcgctta cgtatggtaa tcaagtagaa
    53281 tctacaagga ggtgtcttta agtgagccgt atcggaaata aagtcgttgt tatccctgaa
    53341 ggagtaacag ttactcaaga cggaaacaac gttacagtta aaggaccaaa aggtgaattg
    53401 actcgtactt tttcagcaga tatcaaaatg aatatcgaag gaaacgaagt aactttcact
    53461 cgtccaaacg acagtaaaga aatgaaaaca attcatggaa caactcgtgc gaacttcaac
    53521 aacatggttg taggtgtcag cgaaggtttc caaaaagcgt tagaactaat cggggttggg
    53581 taccgtgcac aattacaagg atcaaaactt gtattgaacg ttggttactc acatccagta
    53641 gaaatcacac caccagctgg agtaactgtt gaagtaccag ctaacacaca agtaattgtt
    53701 aaaggtgcga acaaagaaga agttggcgaa ttagctgcaa acattcgtgg aactcgtcct
    53761 ccagaacctt ataaaggtaa aggtattcgt tatgttggcg aatatgtacg ccgtaaagaa
    53821 ggtaaaactg gtaaataatc gtttagaaaa attgcataga agccagcagc aggaaaaagg
    53881 catttggctg aagtttacct tcaaaaaaat acatttttac ttgtgccgca gcttctattt
    53941 ttcgaaacgt atttactgct tgaaatagta gtacaataaa aataaagagg tgacaattgt
    54001 gattacaaaa ccagataaga acaaaacacg tcaaaagaga catcgtcgtg tacgtaacaa
    54061 tatctctggt actgctgagc gcccacgctt gaacgttttt cgttctaaca aaaacatcta
    54121 cgctcaaatt attgatgacg tagcgggtgt aacgctagca agtgcctcta ccttggataa
    54181 agaaatttca ggtggaacaa aaacagaagc agcacaagct gtcggtaaac tagttgctga
    54241 acgtgcagct gaaaaaggca ttaaagtagt agtctttgac cgtggtggat acctttacca
    54301 tggccgtgtg caagctttag ctgaagctgc tcgcgaaaat ggactagaat tttagaagaa
    54361 ggaggaatac cattcatggt ttatatcgat ccaaaacatt tggaattaga agaccgcgtt
    54421 gttgcaatta accgtgtaac aaaagttgtt aaaggtggac gtcgtctacg ttttgctgct
    54481 ttagttgttg tcggtgacaa aaacggacac gtagggtttg gtacaggtaa agctcaagaa
    54541 gttcctgaag caatccgtaa agcaattgaa gatgcgaaga aaaacttagt tgaagtacct
    54601 atggttggtt caacaatccc acacgaagta atcggtgctt ttagtggtgg tagaatccta
    54661 atgaaacctg ctgtagaagg ttctggggtt gctgctggtg gaccagttcg tgccgtatta
    54721 gaattagctg gggtagcaga tatcacttct aaatctttag gctcaaatac accaatcaac
    54781 gttgttcgcg caacagttga aggtttgaaa caattgaaac gtgcagaaga agttgcagaa
    54841 cttcgcggca aatcagttga agaattaatc ggataaggag gacaacaata atggctgaat
    54901 taaaagttac tttaaaacgc agtattatcg gacgtcctca aaaccaacgc gatacagtta
    54961 aagcgttagg tcttacaaag attaacagtt cagtggttaa gccttctaat gaagcaatta
    55021 aaggcatgat caatactgtt tctcacctag tggacgttga agaaatttaa tttagaaaaa
    55081 tcataactag aacattaagg aggtgccaaa cctatgaaac ttcatgaatt aaaacctgct
    55141 gaaggttcac gccaagtacg taaccgtgtt ggtcgtggta cttcatctgg taacggaaaa
    55201 actgccggac gcggacaaaa aggacaaaaa gctcgttcag gcggcggtgt gcgtttagga
    55261 tttgaagggg gacaaacacc attgttccgt cgtttaccaa aacgaggatt cacaaacgtg
    55321 aaccgtaaag actatgcagt cattaatctt gatgtattga accgctttga agacggagct
    55381 gaagtaactc ctgttgcttt agttgaagct ggaatcgtga aaaacgaaaa agctggaatc
    55441 aaagtattag ctaatgggga actaaacaag aaattaactg tgaaagcagc aaaattctct
    55501 aaagcagctc aagaagcaat tgaagcggct ggtggatcaa tcgaggtgat ctaatgttta
    55561 gactattaaa agatgctttt aaagtaaaag acattagatc gaaaatccta tttactgtat
    55621 ttgtgttgtt tgtatttcgc ctaggtgcac atattactgt accaggcgta aatgctgctt
    55681 cattgcaaaa cctttcttct ttgccatttt tcaacatgtt gaacttggta agtggtagtg
    55741 cgatgcaaaa cttttccatt ttctcaatgg gggtttcacc atacattact gcttctatcg
    55801 tcattcagct tcttcaaatg gacatcgtgc ctcgatttgt tgaatggtcg aaacaagggg
    55861 aagttggtcg taaaaaactg aatcaagcga ctcgatatct gacattagta ttggcattta
    55921 tccaatctgt cggtatcact gctggattca atcaatacgc tcaattaggt ttcgtgaata
    55981 atcctaacgt agcaacttac gtaacgatcg gtttgattct aacagccggt acgatgtttg
    56041 ttacttggct aggagaacag atcacagaaa aaggtatcgg aaatggcgta tcaatgatca
    56101 tttttgcagg tatcatttct agattgcctg aaagtatcag agaaatttat gaagattact
    56161 ttataaatat tgatccttca gacatatgga aatctgctat ttttgtggcc attttactag
    56221 tagcggtctt gttgatcgtg acattcgtaa catttttcca acaagctgaa cgcaagatcc
    56281 cgatccaata tacgaaacgc gtagctggcg cgcctacaag cagctatctg ccgttgaaag
    56341 taaatgcagc tggagttatt ccagttatct ttgctagttc gtttattgct acgccaaatg
    56401 ctgttttaca agcattgagc ggtagattcg gttctgaaaa ctggtacaaa gtgatgatgg
    56461 agatctttaa ctataataca gcacctggtg ctacgattta tacgattctg atcgttgcct
    56521 tcactttctt ctacgccttt gttcaagtaa accctgagaa attagcggaa aacttgcaaa
    56581 agcaaggaag ttatatacca agcgtacgcc ctggtaaggg gactgaagag tatgtctcaa
    56641 gattactgat gcgtttaagt acagttggtg cagtcttcct tggtttagtg gcattgcttc
    56701 caatcattgc tcaaatggtt tgggatcttc cacaatcaat cggattaggc ggtacaagct
    56761 tactgatcgt tatcggtgta gctttagaaa ctgcgaaaca gttagaaggc ttaatgctga
    56821 aacgtaaata tgttggattc attgagtaag aaagcgtgtt gaggtttccc ctcaacacac
    56881 gttctcacac atttattagg agggaaaaca atgaacctca ttttaatggg gctgccaggc
    56941 gcaggtaaag gaacacaagc tgaaaaaatc attgatgctt atggtattcc gcacatctca
    57001 acaggagata tgttccgcgc agcgatgcaa aacgaaacag ctcttggcct ggaagcaaag
    57061 tcttatatgg ataaaggtgc attagttcct gatgaagtaa caaatggaat cgtgaaagag
    57121 cgactagctg aaccagatac agagaaaggc ttcttattag atggctttcc acgtacgcta
    57181 gatcaagcaa aagctctgga cgcaatgcta aaagatttga ataaaaaaat tgatgctgtc
    57241 atcgatatcc atgttggtga agaaatcttg gtcgaacgtc tagccggccg atttatctgc
    57301 agcaattgtg gagcaactta ccataaaatt ttcaacccta caaaagttga agacacatgt
    57361 gatcgttgcg gtgggcatga attctatcaa agagaagatg ataaacctga gacggttaaa
    57421 aatcgtctgg ctatcaacat caaaaatagt gaaccaatct tggcttatta taaagaacaa
    57481 ggtttgctaa acactattga tggtgctcgt gaaatcgatg ctgtatttgc agatgtacaa
    57541 aaaatcattg aaaaatagca cgattgcccg tagccgactt gcattatgca gttactcttg
    57601 tcaagttcaa taaattatga tagaatatgt cagtccgact tctagagaat cctctagatg
    57661 atccattttg agaattttga ggtgggaact gcagtttccg ccattttatc gtgtgaaaat
    57721 gcgaaacaag tacaaggagg tactgtgcgt ggcaaaagaa gatatgattg aagtcgaagg
    57781 tacagtcgtc gaaactttgc cgaatgcaat gtttaaagtt gaactagaaa acggacacca
    57841 agttcttgct actgtttcag gtaaaattcg tatgcattac atccgtatct tacctggtga
    57901 caaagtaacg gttgaattgt ctccatacga tcttaaccgt ggccgtatta cttatcgctt
    57961 taaataattg tactccgtaa aactacagga ggtattatca tgaaagtaag accatcagta
    58021 aaaccaatgt gcgaacattg taaagtaatt cgtcgtaaag gacgcgttat ggtgatttgc
    58081 ccagcaaatc caaaacataa acaacgtcaa ggataattgg aggtgctata aatatggctc
    58141 gtattgcagg agtagatatt cctcgtgata aacgtgtagt agtttcactt acttatatct
    58201 atggtattgg taacactact gctaaaaaga tcttagctga tgctggtgta tctgaagatg
    58261 ttcgcgttcg tgaattaacg aatgaacaaa cagatgctat ccgtgcagaa gtagataaat
    58321 taaaagttga aggggatctt cgtcgtgaag tgaacttgaa catcaaacgt ttgatggaaa
    58381 tcggttctta ccgtggaatc cgtcatcgcc gtggattgcc tgttcgtgga caaaacacga
    58441 aaaacaatgc acgtactcgt aaaggcccag ctcgcgctat cgctggcaag aaaaaataat
    58501 tcttaagtga aggaggttaa aacttcatgg cagcaaaaaa agtgaatcgt aaacgccgtg
    58561 tgaagaagaa tatagaagca ggtattgcac atatccattc tacattcaat aacactatca
    58621 tcatgatcac tgatactcac ggaaatgcat tagcgtggtc atcagctggt tcattaggtt
    58681 tcaaaggaag caaaaaatca actccttttg ccgctcaaat ggcagcagaa actgctacaa
    58741 aagcagcaat ggaacacgga ttaaagactg ttgaagtaac agttaaagga cctggttctg
    58801 gacgtgaagc agcaattcgt tcattacaag ctacaggttt agaagtgact gcaattcgtg
    58861 acgtgactcc agttcctcat aatggtgccc gccctccaaa acgccgtcgt gtttaatgag
    58921 tgccgctcat tttgagtttt atcagtaacg tcattgaaca agacactttt cgttttgaaa
    58981 ggggtaaaga taagaatgat tgagttcgaa aaaccaagaa tcgcaaaaat tgatgaagaa
    59041 aaagattatg gcaagttcat cgttgaacct cttgaaagag ggtatgggac tactttaggg
    59101 aattccctac gtcgtatttt attatcttct ttgccaggtg ctgccatcac taatattcaa
    59161 atcgatggtg tactgcatga attctctacc atcaaaggtg ttagagaaga cgtcactcaa
    59221 atcatcttga acattaaagg cttagcgctg aaaatgtatg gccaagaaga aaaaaccctt
    59281 gaaatcgata ttaccggacc tgctacagta actgctggcg atattatcgt tgatagtgat
    59341 gtagaaattc ttaacaaaga tatgtacatc tgtagtgtct ctgaaggagc tacgttccat
    59401 gctcgcttaa cagtgaaacc tggacgtggt tatgttcaag cagatgaaaa taaaaaagaa
    59461 gatatgccaa ttggcgttct tccagtcgat tcaatctaca cacctgttcg tcgtgtgaac
    59521 tatcaagttg agaacacacg tgttggacac cgtgaagatt ttgataaatt gacgatggag
    59581 atttggacag atggttcaat tgaaccgttg gaagcaatga gtttgtctgc gaaaatcatg
    59641 actgaacatt tggatatttt tgtgaactta actgatgaag caaaaaatgc tgaaatcatg
    59701 gtcgaaaaag aagaaacaca aaaagaaaaa atgttggaaa tgacgatcga agagttagac
    59761 ttgtctgttc gctcttacaa ttgtttgaaa cgtgcaggaa tcaatactgt acaagaactt
    59821 acaaataaat ctgagcctga aatgatcaaa gtccgtaact taggacgtaa atcattagaa
    59881 gaagttaaag caaaattgca cgacttaggt ttaggtttac gtaaagaaga ttaaaacctt
    59941 ttacgaagga gggaaaccaa cgtgagttat cgtaaactag gacgcacatc tagccaacgt
    60001 aaagcgatgt tgcgtgattt aacaactgat ttattaatca acgaacgtat tgtgactact
    60061 gaagctcgtg cgaaagaagt tcgctcaact gcagaaaaaa tgatcacttt aggtaaacgc
    60121 ggagatttgc atgcacgtcg tcaagctgcg gctttcgtac gtaatgaagt tgccagcgtg
    60181 cgtgaagaaa atgaggacat cgtcattgaa tcagctttac aaaaactgtt caacgatatt
    60241 gcacctcgtt acgcagaacg tcaaggcgga tacacacgca ttttgaagac agaaccaaga
    60301 cgcggtgatg gtgcaccaat ggttgtctta gaacttgtct aaaatcacac taataacatc
    60361 actttataga tgattctttt agagtgttat gatgttggaa gatatttttc cgagtctagc
    60421 tcagcgctgc tcctcaagcg aatatcgctt ggggagtaag cactcatcat aaagtgtttt
    60481 tttatacctt caattacctg tcatgacaga tatgtttgaa ggcataaaaa aacattttaa
    60541 gcgagaaata attgaaaatt ttgtcttttt ttgatagtgt tagtaggaac tttaggatga
    60601 agaaggagta atcatggagc caattattga attagaaaag ataaattata aataccagcc
    60661 agatgatctt cgccccgcat tgaaagatgt ctcctttaca attgataaag gtgagtggat
    60721 cgctattatt ggacacaatg gttcaggaaa atcgacactt gccaaaacga tcaatggatt
    60781 gcttctgcca gaatcaggga tcgtgaaagt gggaaatcaa atattggatg aagaaaatat
    60841 ttggacgatc cgacagatgg ttggaatgat ttttcaaaat ccagacaatc agtttgttgg
    60901 ttcgacagta gaagatgacg ttgcttttgg tcttgaaaat caagggattc ccagagaaga
    60961 aatgctagtg cgtgtgaaag atgcacttga gaaagttaga atggctgagt ttgcttcacg
    61021 cgagcctgct cgtttatcag gagggcaaaa acaacgtgtt gccattgccg gcgtggttgc
    61081 cttacgacct gatatcatta ttttagatga agcaaccagt atgctagatc cagaaggtcg
    61141 tgaagaagtg atctctacga tcaagaagat caaagaagaa agtcaattga ccgttatatc
    61201 tatcactcat gatattgatg aagcagctaa tgccaatcgg atactagtga tgagacaagg
    61261 cgaacttgtc cgtgaaggta caccaaaaga aattttttct gctggtcctg aactgatcga
    61321 cttaggtcta gatctaccat ttcctgaaaa actgaagagc gcactgaaag aacgtggggt
    61381 tgatgtgccc agtgaatata tgacagaaga aaggatggtg gattggttat ggacatccgt
    61441 tttgaacaag tagacttcac ttatcagccg aatacaccat ttgaacaaag agccttattt
    61501 gacatcaata tgacgatcaa agaaaacagt tatactgcat tagtagggca taccggaagc
    61561 gggaaatcaa ccttacttca gcatttgaat gcgcttgtta agccaacaag cggtacagtc
    61621 catattggag aacgagatat tcagccggat acggataata agaatttaaa gcccatccga
    61681 aaaaaagttg gcatcgtctt tcagtttcca gaagcacagc tattcgaaga aacggtagca
    61741 aaagacattg ctttcggtcc taaaaacttt ggtgtcagcg aagaagaagc actagtccta
    61801 gcaaaagaaa cattagaaca agttgggctg gatgaaagct atttggaacg ttcgccattt
    61861 gaactttcag gagggcaaat gcgtagagta gcaatcgctg gtgtgcttgc catgaggccg
    61921 gaagtacttg tattggatga accaacggca ggacttgatc cccagggcag aaaagaaatg
    61981 atggagatgt tttggcgtct tcataaggaa cagcagatca ctattgtatt agttacgcat
    62041 ttaatggatg atgtagccaa ttttgcagat tatgtctatg tgttggaaaa gggacgaatc
    62101 gttaatagcg gggagccgca agaggtcttt caaaacattg aatggctaaa agaaaaacaa
    62161 ctaggcgtac ctactgccac cgagtttgca gaagaattaa tggctaaagg gatgaacttt
    62221 gctaccttgc ctttgacagc tgaagaatta gcagacgcta tcgtccatta tgctggagga
    62281 aatgcccatg atgaataaat tgatatttgg tcgttatatg cctggagatt ccttgatcca
    62341 tcgattggat ccccgagcta aattattagc cagtttttat tttatcggaa tcattttttt
    62401 agcaaataat tgggaaacgt accttttgtt ggcatttttc acattacttt ctgtcttctt
    62461 gtcaaaagta gatttgaaat tttttatccg aggcattcgc ccattgatct ggctaatcct
    62521 cttcactgta gctttgcagg tcttgtttac aagtggcgga gaaacttatt ggagttgggg
    62581 tgttttccat atcactgaat ttggtgtgat gaacggcctc tttattttct gtcgattcgt
    62641 gttgatcatt tttatgtcga ctctactcac gcttactaca ccgcctttgg aattatcgga
    62701 tgcgattgag tacttgcttc gtccattgaa agccgtacgg ttcccagtcc acgaaatttc
    62761 attgatgctg tcgattgctc ttcgttttgt gcctacattg atggacgaaa cagaaaagat
    62821 catgaacgct cagcgcgctc gcggtgttga ttttggtgaa ggaaacctag ttcagaaaat
    62881 gaaagcagtc attcctttgc tgattccttt atttgtcagc agcttcaacc gtgcggaaga
    62941 tcttgcgact gctatggaag caagaggcta caaaggtggg gaaggaagaa cgaaatatcg
    63001 tgtattgcat tggcataata gagacacgat ggttatcatt gcttttgtat tattaactgt
    63061 gctactgatc tttttaagag gataaacgtt cagtgggccg ctatctattc ttttcttgct
    63121 gatctgtttg caagaaagga tgcaagcagt gtatgacaag aaatattagg gtctgtggca
    63181 gactaaggtc acaggcctct ttcttttatc tgaaaagaaa gaacgaaaaa attttataga
    63241 aaaatcatta ttccagataa ccttttagga acaaaggaga gaaaaacatg gtacgttaca
    63301 aagcaatcat tgcgtacgac ggaacaaatt ttaatggatt ccaaaaacag ccaaatgggc
    63361 gtactgttca agaagaagta gaaaaaacat tacaaaaaat ggcaaatgga aaagaaatca
    63421 cggtctttgg ttcgggaagg acagatgccg gagtacatgc gatcgggcaa gtaatccatt
    63481 ttgattatcc agaggagcgt ccgttagaac gaatgcgttt cgcgctagat acccagtcac
    63541 cagaggatat tgctgtaaga caagtagaaa ttgtttcaga agacttccac gcaagatatt
    63601 tggtcaaaga aaagacgtat caatttcgag tggatatagg gaaaccacgc agtccttttc
    63661 gacgccatta tgcaagttat tttccttacc cacttgattt ggaaaaaatt cagcgggcgc
    63721 ttcctgattt attagggact cacgatttca cctctttttg tgcatcagga agctcgatag
    63781 aggataaaat acgtacgatc tacgaggcta agatggaggt aaatgaaaca aaagacgaat
    63841 tgttgtttac atttagggga aatggcttct tgtataagat gatacggatc ttagtaggaa
    63901 cgcttttgaa aattggcaat ggccgattgc ctgaagatag tatcccagat attattgcga
    63961 aaaaagatcg taatgcagca ggacctacag cacaccctga aggactttat ttgtatgaag
    64021 tgaaatatga ataagaggtt atgatagaag tggtcagctt caagaaataa gaaggtattt
    64081 acgaaaatta cttctcaaaa ttttgcaaaa tttcggctta ttttcgaaga agctacttct
    64141 ggaacaccgt ttaagaaaga ggtcgtgagc ctgtcattta actctgagtc acaaagagca
    64201 atttcaaaaa acagcttctc atctttgatg aatatcgtga cttgtggacg aggcgttgat
    64261 aagtgaatca tctttttgtt acctattttt tctgtaacca aaaaacgatg gaacgttgtt
    64321 ttgattgccc ttggtgcgga cagtctgaac aggttccatc gtttctttat tttctacgtc
    64381 ttcttctgtg tgtgtttcgt ggttgttctt ctattgctgg atcaatcgtg ccataaaagg
    64441 ggcgattgcg gtcaaggtac cagacataaa gaataaacaa tgcaatacat aggacaaaaa
    64501 ggattgcacc tatcgtaaat ccttcaggca agtaactgaa agtgacagta tgtttacctg
    64561 ctggaacttt tatcatcaag aatgcgtctt tgaaattgct gaccttgact ggtttcccat
    64621 caattttagc tttccatcct ttgtcataag gaatagttgt caccaacatg gaatcttcag
    64681 cggtatttac agtcccagaa gcttttctgc cagtggcttc catttcgaca cctttttctt
    64741 tgatctgatt cattgcggct tgataggcta caacatctag accaacgact ttaggctcca
    64801 tgaaacttat ttctttcgtc ccataaaaac ttgcagtaaa tgtcactgtt gtctcttgag
    64861 gataatagcc aagatcatag tattgaccag aaatgttgat ttgcgttttt cgcgatgtac
    64921 cattgacagt gatcgtcgca ctagaacttt ctaattgcgc aaaattggtt gggaacaaac
    64981 ttaagtaagc ttgtgtattt gcaggcacat ggacttccca agtgagctct tttggctggt
    65041 tatctgctgt ttctgagtaa gtgacgccag tcgttgtctt tgtgatcgtc gtattcaact
    65101 gattcttcaa agtaggtgta tagaatgtaa aatactgttc atttagacca gacaatgtat
    65161 tgatcaagtt tgtctgactg gtcaaattat ctgtcactgg ctgctcaaca tcattaacgg
    65221 acaacggtgc agtaaatgct agtggcaatg cattgacatt ctgatacagt gtatagtcgc
    65281 cgctttgacc agcttggcgg aagccatatt tactgaacgt gctttcgttt ttagaaatgt
    65341 tatatttgat tcctgtaaac ccatccatca gcaaagtatt attgttataa cggatattca
    65401 aatttgtccc tcttgaacgg aaacctaatt tatccaggta agaggaagaa ttacgatttc
    65461 ttatagaaga aaacaaactg ataccactgt aaccatagtt gatcgaatcg tttgcagata
    65521 ctggatccag attttccaag cggtaaaacg agtcggaatc tttttttgtt tcatttacaa
    65581 gagctttcaa gtcaggataa ggatcagtat ataaactgcg ggaagcataa ttccaatcat
    65641 caagaatgcc atggatcata ctgttcgtat tgaccagtgc ttcagcagac atgatcaata
    65701 ataacaatat actcagatga ttgaatgaaa gttttttatg ctgataaaat cccacagcaa
    65761 aagcataaat ccctaagaaa accactgtca atacaaagga agtcatcgtc acataggtgt
    65821 agcttccttc aggtttgatg ccccatgcca ccgcaaagag agcagctacg accaaaatga
    65881 ttcctaacaa cagcccacca tcgtcttgtt ctatcttttc ccatcctttt cctgcaagca
    65941 ggagcactaa gaaagagaac agataggcat aacggaataa aaacatattc ggtgcatgca
    66001 taccatgcca gaacaaattc aatggaacaa gataaaaact tgcgataagt atggcgaaaa
    66061 gactgccgta caaaaattta tttttcaacg gtactttccg actggcaaaa taaaacagac
    66121 agaggactaa tggtagcaga cctgcgtaaa cgaacggaat cgatccgtat tttgtcgtgt
    66181 cataaacacc aatcatattt ttcatgatta gatccagata agaagtcgct tctgttttca
    66241 acgtagtgat ctgactcagt gcctcgccat ttgaccgaag atccagcact gctggcaata
    66301 caatgatcat tgaagcgccg cctgccagta aagaagtgat accataagga acaatcgttt
    66361 ttttatattc tgaccagttt gtcaatagac gggcgataaa atataagaaa gaaaatacgc
    66421 caatcataaa acccatataa aaactggata aaaacaagag taaataactg ataaataaaa
    66481 gtttcggccg tttaagatcc ataacacgat gaatccccaa aatcactaat ggaagataaa
    66541 caaatgcatc cagccacatg atgatctccg aatgtgcagt gataaatgag atcaatgcgt
    66601 aactgacgct aagtgcaaca tgcccccagc gaggaatctt gaacgtacgt gcaagaaacc
    66661 aaaaagccaa tccggcacag ccgaccttaa gcaaagtcag aacatacagt gcatctggca
    66721 tcatctggtt tggaaaaaag aacaccagtg gcgtgaagat cccgcctaga tagtaagaaa
    66781 ttaaagataa ataattcaat cctagagaag cattccatgt ataaaaaata ctttgctttc
    66841 caagaagaac atttcggaag ctggcatgaa aattagaaaa ctgtgaaaag gcatcacttg
    66901 ccaaaatact tctgctgctt ccaggataga ttccgatact tagataaaca agtgctaaaa
    66961 tcaaaaacgg aatcaggaaa ctagctgctg cgtagcgtcc gttttctcta aagaatgact
    67021 ttaaattttt tttcatattt attttccacc aaatttctat gaagttgtat tcagtttatc
    67081 atatccttgt ttttctctgc ttaaaaagag tctagttttc acaaaaaaat aaaatttaga
    67141 ataaataact tcataatttg ttcgtataac aaaaataaac gcaaaaaaag ggtaggaatc
    67201 aaaagtccca cccttaaaaa tttttattaa ataaaaaacc gttcactatc tgaacggtcc
    67261 aagtatcatt caattaaacg cgttcaactt ttcctgattt caaagcacga gttgacaccc
    67321 aaactttttt aggttttccg tcgaccataa cgcgaacttt ttgtaagtta ggtttaacag
    67381 tacgttttgt tgagttcata gcgtgtgaac ggttgtttcc actttttgtc ttgcgacctg
    67441 taaagtaaca aacttttgcc atggtaattt cctcctttgc cttgatttgg agcaatagaa
    67501 taattcagct cataccagac taacatatca tatagaatgg gaatttgcaa gtacattttc
    67561 atagatacaa ttgacaattg agaagaataa ttgtaaatta aacaaggtta tacctgaaat
    67621 tttgcaaggt ttttgagtac tttgggtaac tgccttttat ttcaagttgc gctatgataa
    67681 aatatgatag aagaaaaatg ggagtttttt acccgttaag gaggctcatt catggctgta
    67741 aaaattcaaa cgtcagctgg aaccattgaa attaccaatg atgtaattgc aacagttgtt
    67801 ggtggcgccg cgacagatgt gttcggtgtt gtaggaatgg ccagcaaaaa tcaaattaaa
    67861 gataatatta ctgagatttt gcgtaaagaa aattactcaa gaggagtagt tgtccgccaa
    67921 gaagataatg gtattgctgt agatgtttat acgattatca gctatggtac gaaaatctct
    67981 gaagtttccc gcaacgtcca agaaaaagtg aaatacaatc tagaggcatt attaggcgta
    68041 actgccaatt cagttaatgt ctttgtacaa ggtgttcgtg tactgccaga ctaggcagga
    68101 gactggctgg taactcagca gtcgatctga aggaggatta tctaggtgag tgtaacagaa
    68161 ataagtgcaa gccagttcca ggaaatggtc caggcaggtg cgaatcgtct gcaagtaaat
    68221 gcagaatacg tcaattcatt aaatgtattc cctgtgccag atggcgatac gggaacaaat
    68281 atgaatctat ctatgactag tggagctaag gcagtagtag attcaacctc agaaaaagta
    68341 ggagaattag cagcaatgct ttcaaaagga ttgctgatgg gtgcgcgcgg aaattccggc
    68401 gttattttgt ctcaattgtt tcgtggattt tctaagcaaa ttccagatgt tgtctcatta
    68461 aatgcttctg atcttgctgc agccttcact catggagttg aaactgcgta taaagcagta
    68521 atgaaaccag tagaaggaac gattttgaca gtagctagag aagcagcgaa agctggagaa
    68581 aagaaagcaa aaagtactga tgatgtcgtt gaagtaatga cagctgttgt aaaaggagca
    68641 aagcgtgctt tagctaaaac tccagacttg cttccagtcc taaaagaagt cggtgtcgta
    68701 gacagtggag ggcaaggact gctgtttgtt tatgagggct ttttaagtgc attgaatgga
    68761 gaattccaag cggatgaaac atatgaacct tctccagcag aaatggatga tatggtcaat
    68821 gcagaacatc atcgcagtgt ccaaggtcaa atggcgacag aagatatcca ttttggttac
    68881 tgtacagaaa tcatggtaaa aatcggagaa ggacctacag tcgatagtac attcgattac
    68941 gaagaattcc gtaactattt agatggtata ggagattctt tacttgtggt caatgatgac
    69001 gagatcatca aagtccacgt gcatacagaa aacccaggag aagtcttgaa ttatggacaa
    69061 aaattcggtt cattagtcaa aatcaaagtc gacaacatgc gcttgcagca tgagacgatt
    69121 ttggaacacg atgaagaagt gaacgagttt gcgaaaaaag aaataccacg tatcccgtat
    69181 ggtatcgtag cgatcgctgc tggtaaaggc gtccaagagc tgtttgaaag tttaggcgct
    69241 aattatgtta tcagtggtgg acaaacaatg aatccaagta cggaagacat tgttaaagcg
    69301 attcaagaag taaacgcaga taaagtgatt gtcttgccaa acaataagaa catcttcatg
    69361 gcagctgatc aagcagcaga agtagcagat atcccagtag cagttgttcc ttcaagaacg
    69421 atttcccaag gattgacagc tatgctttca ttcaatgatc aagcttcttt agaagaaaac
    69481 aaagaaacga tgacagaaat gttgtctagc gttgtgagcg gtcaagtcac tcacgcgatc
    69541 agagatacct caattgacgg tgtagagatc aaagaaggag actttttggg catgatcgat
    69601 ggaaaaatcc tgatctctaa cccagatatc acagcaacag cgttaagtac actagaacaa
    69661 atgatcaatg aggacacaga gatcgtaacg atcttggcag gtgatgaagg aacagttgaa
    69721 caagcaaatg aatttgctga gaaactaaca gaaaaacatc ctgatttgga aatcgagatc
    69781 caccaaggag atcagcctgt ctatccttat ctattttctg ctgaataatt tctgtgtcag
    69841 aatatctttc taacacagta ttcattcata aaaggccttc ggttagtgat caggtatgat
    69901 cgctgaccaa gtcctttttt tgttaaagga gggaggaaaa atttatgcgt tctttagatg
    69961 atccaatcac tatgctggca ggagtcggag aaaaaagagc agctagtctt gccagcttgg
    70021 gcatccaaac gattgaagac ttactgactt attatccttt tcgctacgaa gatattcagg
    70081 aaagaaatct aaacgagatc caggatcaag aaaaggttac tttgaaagga atcgtagtct
    70141 cgcctcctgt gatgaatcga tttggttata aaaaaagccg attgcaattt cgtatgatgc
    70201 aagaacatga tgtgttcaac gtttcttttt ttaatcaacc ttatctaaaa gaaaaagtca
    70261 ttttatcgga ggaaatcgcc gtatacggta aatgggatgc aaagcgtaaa gccttgaacg
    70321 ggatgaagat tttaggttct caaacaatgg atgacttttc gccgatatat cacgtcaaca
    70381 aatcaattcg tcaaacgaca ttagttgatc taatcaaaaa agggtttcaa gaatacggca
    70441 cattgattga agaaaatctg ccaaacaacc ttgtggagaa atatcgactg cttgaccgag
    70501 cttctgctgt aagaagtatg cattttccaa aagaccacga agaaaaccat caagccaaac
    70561 gacgtgtcgt ttttgaagag ttctttttat ttcaaatgcg tcttcaagga ttgaaaaaag
    70621 cagaaaaagc agaaacgaat ggtttggaga tcctttatga tgtccaaaaa ttgaaacaat
    70681 ttactcaaaa gctacctttt gaactgacaa atgcacaaaa acgtgtaacg aatgaaatct
    70741 gtcgggatct tcgcagtccg cagcatatgc agcgactttt gcagggagat gtaggtagcg
    70801 gaaaaacagt ggttgccgcg attgcgttat acgctaccgt tacagcaggt ttccaaggtg
    70861 cgttgatggt gcctacagaa atcttggcac aacaacatat ggaaagcttg caacagctgt
    70921 ttgatcccaa tgaagtaacc atcgcattga ttacggggtc aacgaaagca aaagaacgac
    70981 gtgagctcct tgaaaaacta gagcaagggg aaattaacgt cgtgatcggc acacatgcat
    71041 tgatccaaga tggagtcgaa tttcagaatt taggtctagt tattacggat gagcagcacc
    71101 gttttggcgt gaaccagcgg aaagtacttc gtgaaaaagg ctggcgacca gatgtcttat
    71161 ttatgacagc gactccgatt cctagaacat tagctattac agcatatggt gagatggatg
    71221 tatctatcat tgatgaactt ccagctggac gtatcccgat cgaaacacgc tgggtaagaa
    71281 cacctcaatt ggacagtgta ctggattgga cgaaaaaaga actggcaaga ggacatcaaa
    71341 tgtatgtcat ctgtccgctg attgaagaat cagaagcatt agatgtaaaa aatgcagtgg
    71401 aaatctatga aaaacttcgt gaactatttg caccaaaata tgaagttggc ttgttacatg
    71461 gtaaaatgaa gaatcaggaa aaagaagtaa tcatggaagc cttcaaagac aaccagatgc
    71521 agatattagt ttcgactact gtaattgaag taggggtaaa cgtgccgaac gcgaccgtca
    71581 tgctgatcat ggatgcggat cgatttggat tggcacagct gcaccaactt cgtggacgtg
    71641 ttggacgcgg aagtgatgct tcctactgta tcttagttgc taatcctaaa aatgaactag
    71701 gtgtggaacg gatgaaaatc atgacagaga ccaccaatgg ttttgtacta agcgaaaaag
    71761 atctggaatt acgtggtcct ggtgaagtat ttggcttccg tcaatctggt ctgcctcaat
    71821 ttgcgattgc tgacttggtg acggatggaa atgttttgga ggtagccaga gaagaggctg
    71881 cagccctttg gcaaataaag gattggcaga tgcttcctga gtatcagcct ttagcgcaaa
    71941 gcctccaaat agaaaatcag gataatcgtt atttcgatta gcagactgtg ctatacttta
    72001 tactgatgac ggaaaaagga gtggaaacaa tgaaaattgc cgtagatgca atgggcggag
    72061 atcacgcacc tcaagcaatt gtagaaggcg tggctttagc gcagcaagat tttccagaag
    72121 tagaattctt actttacgga aaagaagcag aaatcaagaa atacttaaca aatgaaaaaa
    72181 acatcacgat tatccataca gacgaaaaaa tcaatagtga tgatgagcca gtaaaagcaa
    72241 ttcgacgaaa aaagactgca tcaatggtcc ttgctgcaca agcagtgaaa aacggtgaag
    72301 cagatgctgt attttcagca gggaatacag gcgcgctact agcagcaggg ttgttcatcg
    72361 taggacgtat caaaaatatc gaacgccctg gcttgatgtc taccttgcca gtagtaggga
    72421 aagaaggtgc cggtttcgat atgctggatc ttggcgctaa cgcagaaaac aaaccagaac
    72481 atttgttgca atacggtatt ttaggttctt tttatgcacg taaagtccga ggtatcggac
    72541 gtcctcgtgt cgcactctta aacaacggaa cagaagcaac aaaaggtagt gaagtgacga
    72601 aaaaagccta tgaactcctt cagaatgaaa caagcttgaa ttttattgga aacgtagaag
    72661 ctcgtgagct actcaatggt gtggctgatg tagtggtcac agatggattt acaggcaacg
    72721 ctgttttgaa atctattgaa ggaacagcaa tgaatatgat gaatctatta aaatctgcta
    72781 ttctaaatga agggatcaaa ggaaaaatgg gcgctatgct tttaaaagac ggcttacgtt
    72841 ctttgaaagc agaaatggat tattccaaac atggcggtgc cgttttgttt ggcttgaaag
    72901 caccagtcat taaaactcat ggagcaacag gccccgatgc tgttcgctat acgatccgcc
    72961 aaatccacac aatgcttgag acagatgtag ttggacaatt agtcgaacaa tttgaaaaaa
    73021 gcgaagaata gtatttggat atctgaagcg atccgttcca ttgaaaaaaa ggtggacatt
    73081 cctgaaaaat gccagtaaaa ttagccatgg tgtaggtaat tgacaagtgt atgattcttg
    73141 gaggtgaaaa agttgactcg cgaagaagta tttaacaaag tagcgaaaat tatttcaaat
    73201 cattttgaga tagataccga taaagtgacg gatgaattga acatcaaaga tgatttaaaa
    73261 gctgattcaa ttagtattat ggaattcgtt ttagagcttg aagatgaatt cggtacggaa
    73321 atttcagatg aagacgctga acaaattgaa acagtcggtg gcgcagtaga ttatatcagc
    73381 agccatctta aataatttca aataaaagca aagaaggata aaaagctgca tgcagctttt
    73441 tatccttctt tttttaatct ttttgacacg taaaagtcat ttttctgata attctggcga
    73501 taaacagaaa ttatgttgca aagtaaagcg gttttctata taattattaa taattcatta
    73561 cacaaagata agaataacct ttgttataaa acgatttatt ctgtgtaatg tcctttttta
    73621 ttaagaaaaa cttccattta ggaagcagag aggggtattc gattgtccga taaccaatta
    73681 ttagaagttc agaacctaca tacaggattc cgactcaaag atgactatta cgatgcagta
    73741 gatgacgttt catttacttt agacaaaaac gagattttag caatcgttgg ggaatctggg
    73801 tgcgggaaaa gtaccttggc tactacgatt atgggattac ttgatcctaa taacacaaaa
    73861 attacaggtg agattttata taacgatctg aatttaatcg acctgaacga aacgctctat
    73921 aataaaatcc gcggaaatga tatcggaatg attttccaag atccgttgtc tgcattaaat
    73981 cctctcatga gaatcgaaga ccagatcaaa gaaggacttt cctatcacac aaagatgaat
    74041 gcagaacagc gtcaagctcg tgcactagaa ttgctagaac aagtagggat tcctaatcct
    74101 gcgagagttg gacgccaata tccccatgaa ctatcaggcg gtatgagaca acgtgtcatc
    74161 atcgcaatcg caatcgcctg caaacctcca atcgtcattg cagatgaacc gacgacagcc
    74221 ctagatgtaa ccatccaagc acagattctt gatttactga aagaccttca ggaagaaaca
    74281 aaaacaggga tcatcttgat cactcatgac ttaggagtcg tagcagaaat ggctgatcga
    74341 gtggcagtga tgtatggcgg acagttcgtt gaagtagcag gtgtaaaaga actgtttgat
    74401 catcctaagc atccttatac acgctcttta ctgaactcta taccacaaga aggaaatcat
    74461 gatgcagaac tccatgtgat cgaaggtgtc gttccttctc tgaaaaacat gcctcgtaaa
    74521 ggttgtcggt ttgcaccacg tattccatgg ataccagcaa gcgatcatga agaagagcct
    74581 atccttcatg aagtcgcacc gaaccacttg gtacgttgct cctgctataa gcatttccac
    74641 tttagagacg aaaaaggaga ggtgtagatg acagaattaa tcacaatcaa agacctgaaa
    74701 gttcattacc ctattcgcag tggattcttc aatcgagtaa cggatcatgt atatgctgta
    74761 gatggtgtgg attttattat cgaaaaagga aagacatatg gtttagtggg agagtctggc
    74821 tctggaaaat ctactacggg aaaagccgtt gtgggattag aaaaggtaac agccggacaa
    74881 atcatgtatg aaggaaaaga tgtaacgaag cgaagcaacc gcaaaaaaat gggttataac
    74941 aaggatgtgc agatgatctt ccaagattca atgtccagtt tgaatcctaa aaagcgagtg
    75001 ttggatatca ttgctgaacc catccgaaat tttgaacggt taagcgatca agaagaaaag
    75061 aaaaaggtaa aaggactgct agatatcgtt gggatgccgg aagatgcgct atataaatat
    75121 cctcatgaat tttcaggcgg acaacgtcaa cgtctaggag tagcccgtgc tgttgctacc
    75181 aatccaaaac tgatcgtagc cgatgaacct gtatctgctc tggatctatc tgttcaagcg
    75241 caagtattga atttcatgaa acgaatccag caagaatacg gcctcagcta tttattcatt
    75301 tcacatgatt taggtgtagt aaaacacatg tgcgacaata tcgcaattat gtacaaaggt
    75361 cgatttgtag aaatcggtac aagagaagac atttataatg acccgcgaca tatctatacg
    75421 aaacgtctgc tttcagctat tccgcaaatc gatgtagaaa atagggaaag ccacaaagaa
    75481 aatcgacgaa gagtagaaca agagtatatc caaaaccaaa aagaatacta tgatgcgact
    75541 ggtcgagtct acgatttgcg tacgatcaca actacacaca aagtggcgtt gaaagatgga
    75601 ggtgcaagct aatgtggaaa acgattctgc gacgtgtttt attgatgatc ccgcaagtta
    75661 ttattttaag tgttctgatc ttcttattag cccaagcgat gccgggggat ccatttaccg
    75721 gtttgatcaa tccaaatcag gatccggcag tgatcgaaca gatgcgacaa gcagctggct
    75781 tgaatgaccc atggtatgtc cagtacttcc gttggatcgg aaatgctttg caaggcgatt
    75841 ttggtcaaag tttcttatac aaacgctctg ttgctgactt gatcggcgaa cggatcatca
    75901 acactttata tctatcgatc ttgacagttg tccttactta tttgattgct gtgcctctgg
    75961 ggatgcttgc tggccggtat caaaattcaa tcttggataa gattgtcgta atctacaact
    76021 tctttagttt tgctgttcct ttatttatct tcggcttgat catgctattt gtttttggtt
    76081 atcgtttagg ctggttccca acaagtggat cacaaacact aggtttgcaa ggtggattta
    76141 tcactcaatg gatagaccgt ttgaaatata ttctgttacc atcaattacc caagcatttt
    76201 tggcgactgc cgtcacaatc caatatctgc gaagtgaagt aatcgattcg aaatcgctcg
    76261 attttgtcag aacagcccgt tccaaagggg taccgacgaa taaagtattc agtcgacata
    76321 ttttccgaaa tgcagcgttg ccgatggctt ctcaaatggg gtatgaaatc acttcgttga
    76381 ttgctggttc tgtagttatc gaaaagattt ttggttatcc gggagtcgga aaactattta
    76441 ttgattccat cggccaacgg gactataccg tgatcacatc cttagtattg attttaggaa
    76501 tcgcgacgct agtggggaca ttactttcag atatcatcat gagtatcgtt gacccgcgta
    76561 ttcggattca ataaaaccaa tcagaaagga gaatggatcg atgagtgaaa aaaacaatac
    76621 agcagttcaa gaaagtattc ctcctatggg gatacgaatg attgctcgag aatttaaaaa
    76681 agataaaatt gctatttttt cattaggttt gctagtggtt ttattattag tgatctttat
    76741 cggtgcgatg ctgaccgatc aagacaaagt gatgtatgtt agtatcttcg acaaatacgc
    76801 tgcaccagga acagtttcga cacaaggaca aaagttcatt ctgggagcag atgaaggcgg
    76861 acgtgatgta ttaggacaat tgatcatagg tgctagaaac tctgttacga tcggatttgc
    76921 gatcacgatc cttacttcga tcatcggggt tggattaggg atcatctctg gctactacgg
    76981 agggatcatt gacaacatct tgatgcggat cgttgatttt atcatgattc taccgatcat
    77041 gctgatcatt atcgtcttcg tatcgattat ttccagatac aatgtctggt cctttatatt
    77101 catcatgagt gcattttatt gggtagctaa agcccgatta ttccgaagca agacattatc
    77161 tgaagcaaga agagattatg tcagtgcctc aaaaacaatg ggaaccaatg attttaaaat
    77221 catgttccgc gaaatcatgc cgaatctaag ttcattaatc attactaatt tgacgatgaa
    77281 ctttgcggca aacatcggta tcgagacaac tttgactttc ttaggtttcg gcttgccggc
    77341 aaatgtacct agtttaggta cattgatcgg ttatgcaagt aacggagaag tcttagtgaa
    77401 tcggcaatgg atctggttac ccgcatcaat ccttatttta gttttgatgt tgagtatcaa
    77461 ttatgttgga caagcattca agcgttctgc agatgcgcgc caacggttag gctagtatag
    77521 aagcgaaggg ggaaatagga atgaagaaaa cgatttttgg atttgcagcg ctgatgtctg
    77581 ctgtcgttct tgcagcatgc gggggcggag gtacagatac ctctggaaac aactctggag
    77641 gcaacggcaa taacaaagag gaattaactt ttccaattgc aacaaaaaat aatgatgatg
    77701 caatcgaaga cggcgaatta gaagctgcgg tcgcaacgga cagtcagttt aaaggattgt
    77761 tccagtggga gttttaccaa gattcatatg atgcagcttt tatggctcct tctcatgaac
    77821 cgctatttgc aaacgatgac gatttcagaa tcacagacga cggagctgca acgcttgatc
    77881 tagatgaaga tacgaaaaaa gcgacaatca aagtaaaaga caacgtcaaa tggtcagatg
    77941 gtgaagatgt aacggcggac gatgtgattt tcccttacga aatcattggg aataaagatt
    78001 acactggtat ccgttatgac gacactttcc gtaacatcgt aggtatggaa gaatatcatg
    78061 atggaagtgc cgacacaatc tccggaatca aaaaaataga cgacaaaaca gttgaaatcg
    78121 aatataaaga agtaaaccca agcatgcttc aatcgggtgg tggtatctgg tcatatgcag
    78181 caccaaaaca tacgttagaa ggtgtagcaa tcaaagatat ggaatcaagc gatcaagtac
    78241 gtaaaaatcc agtgactttt ggcccttatt acatgagtaa tatcgttgca ggagaatcag
    78301 tagaatactt gccaaacgag cactactgga atggtacgcc taaattgaaa aagatcacga
    78361 tgaaatctgt accgatggca agtgcaactg aagcattgaa ctctaaatta tatgatatga
    78421 ttttccaaat gccaacagat acttacgata cctataaaga aattgctggc tatacaaact
    78481 taggccgtga acaaacttct tatacttatc taggattcaa gttaggtaaa tggaatgcag
    78541 atacaaagag tgtggaatac aatccagatt caaaaatggc tgataaatca ttacgtcaag
    78601 caatgggcta tgcattagat aataatgcag taggtgaacg tttctatgca ggtttgagaa
    78661 gcaatgcgac ttcattgatt cctccagtat tccagtcata ccatgatgat gagatgaaag
    78721 gattcacaca agatctagac aaagcgaaaa aactattaga tgatgctggc tacaaagatg
    78781 tggatgacga tggtttaaga gaagacaaag atggaaataa actaacaatc aactttgctt
    78841 cgatgtccgg tggggaaaca gcgcaaccat tggcagatta ctatatccaa caatggaaag
    78901 aaattggttt ggatgtccaa tacacaacag gtcgtctgat cgaattcaat tctttctatg
    78961 ataaattaga aaacgatgat cctgaaattg atgtttacca agctgcttgg aatacaggaa
    79021 ctgatccaaa tccaagtgga ctatggggac caaatgctgc gttcaactac actcgttatg
    79081 aatcagaaga aaatacaaaa ttgatcaacg atatcagttc aagcaaagca tttgatgaag
    79141 agtaccaaaa agaagcatat caaaaatggc aacaatatgc atttgaagaa gcatttgcga
    79201 ttccaacgtt gttccgtaat gaagtattgc cagtcaacga ccgtgtcaaa gactgggatt
    79261 ggtcttatga tgtaccaaac ccatgggcaa atgtttctgt tacagcagat tcacgttcat
    79321 aatcactaaa aatgaccgaa aaagggcgat gtcctttttc ggttttttga tatttttctg
    79381 tttatttctc taatattcag gtcaaggcgc tgatttcatg acttctccaa taaacggtgt
    79441 tcaaagtcca gtttctcggt attaagtcaa atttatgcaa aaatatggaa agccattttc
    79501 acaaaatttt ttcttcatac tcgaaactaa acgaattttt cacaacttct cgtcaatcac
    79561 taggattatt aaagctttac ttaccttttg accgaaccat ttcaaatttg acaaaaaacc
    79621 agtataataa ggaagtatcg aaaaaatatc cataaaagga atgtggaaaa tggacaacca
    79681 gctgacaaaa gaactaaaag aaaactataa catcgttttt cacgatctaa atttgcttga
    79741 acaagctttt acccattcat cctatgtgaa tgagcatcgc aatctgcaat tatcggataa
    79801 tgagcgactt gaatttttag gagatgccgt tttagaactg atggtttctc agtatttatt
    79861 ccgtttatat cctcatttgc cagaaggaaa attgacaaaa acacgagctg cgatcgttcg
    79921 cgaagacagc ttgtcgaaat ttgccaaaga atgtcacttt gatcaatatg ttttgttggg
    79981 caagggagaa gagaattcag gaggacgtac gcgaccagca ttgctttgcg acctattcga
    80041 agctttttta ggcgcgttat atcttgacca gggattcgac acgacccatg cattcatcga
    80101 aaaagtgatt tttcctaaag tcaaagcagg tgctttttca catgagatgg atcataaaac
    80161 aaaactacaa gaagtcttgc aaaaatcagg tgatgtatca atcgaatacc gattgatcaa
    80221 tgaagaaggt ccagctcatg aacgagtatt ttggatcgaa gtttatgtag atgatcaatt
    80281 gattggcact ggacaaggga aatcgaaaaa attagcagaa caagctgcgg cggaaaatgc
    80341 attggcagca ctgtaaaagt aagtgaggga aagctttgta tttgaaacga attgaaatag
    80401 ccggttttaa atcatttgca gacaagacag tcattgattt tgaaaatagc gtcacagcag
    80461 tggttggacc aaatggcagc ggaaaaagca atattacaga ggctatccgc tgggtattag
    80521 gagaacaatc tgctaaaagt ctgcgaggcg gaaaaatgcc ggatatcatc tttgccggtt
    80581 ccgatacccg taagcagctg aatattgcag aagtcacagt gattttagac aatagtgatc
    80641 attatctgcc attggagtac aatgagatca gtgtaacccg ccgttatcga cggactgggg
    80701 aaagcgagtt tttcatcaat aaacaaagct gcagattaaa agatatccaa gaattgttct
    80761 tagattctgg attaggaaaa gaatcgttct caatcatttc tcaaggaaaa gtagaagcga
    80821 ttttcagtag taaaccggaa gaccgtcgtg ggatctttga agaagcagct ggtgtcttga
    80881 aatacaagca gcggaaaaag aaagcagaac agaagctttt tgaaacagaa gacaacctca
    80941 gccgcgtcca agacatcatc cacgaattgg aagaacagtt aactcctttg gccgcccaga
    81001 gtgaggcagc aaaggagttt ttacggctaa aagaaacact gactcaaacc gatgtctctt
    81061 tgatggttgc tgaaatcaag acagcaaaaa aagattggga caacaaacaa gcacagcttg
    81121 cgaaattcaa tctagaatta ggcaaactat ctgagtcgat ccaagaacaa gaatccattt
    81181 tggcgaagca gcgtaaagaa aatgcgcaag ccgatcgtct gatcgaaaaa aatcagcaag
    81241 tattattaga tctttcggaa aaactgaagc agacagaagg acaaaaagat gttttgcagg
    81301 agcggacaaa gcatacacaa aaaagctcgc aagaatatca aacttcatta gcagaagcac
    81361 aaaaaaaggt taaacatttt gaaaagcttc aagaaagctt gatgaaagca gcagcagaaa
    81421 aagaaacaga gatccaaaaa gctgaagcaa atctcataaa aacacagcaa gaattagaaa
    81481 aataccaaaa atcaacgaaa gaattattgg cagaattaag agatcaatat gttgatttga
    81541 tgcaagaaca agcagctgta ggaaacgaac tgaagtatct agaacgtcaa tatatacaag
    81601 aaacagcaaa aagcaagcag acgttagcca agcaatcgga agtagaagca tcagtagacc
    81661 gattgatctt acaaaaagaa gaattgacac aaaagcaggc gcagctaaaa tcatctttaa
    81721 ctgaaacaaa agagaagtta gaaatgatcc aacaaaacgg gaaaaagttc caagaagcat
    81781 tagcaaaaga acagcctaaa atgtatcaat taatgaatca agtccagcag ctacgtgctc
    81841 gtaagaaaag ccttcaagaa atccaagaaa actattttgg tttttaccaa ggggttcgat
    81901 tggtcttgca gcataaacag cagctttcgg gtattgtcgg agcagtggct gaactgatcg
    81961 atgttcctgc agatttcacg ttagctatcg aaacagcttt aggtggggca gcccagcatg
    82021 tcatcgttga aaatgaaaaa gatgctcggc aagccatcac gtatttgaaa cagcaaagag
    82081 gaggaagagc aacattcctt ccactgacga caatcaaacc aagacaatta ccggctcata
    82141 tattgacaca agcagcagct gtagaagggt ttatagggat tgccagtgaa caagtcagtt
    82201 atcccgatca aatccaaact gtggttcaaa acctgctagg aacgatcctt ttagccaaag
    82261 acttaactag tgccaatgca atcgcgcaaa cgatccgcta tcaatatcga gtggtttcct
    82321 tagaaggtga tgtgatgaat gccggaggat ccatgacagg tggagcgaat aaacgaggaa
    82381 accaaggcag tttgtttgta caaaatcaag aattgaaaca gttgactagt gaatttgaag
    82441 aagcagataa acaactccaa gcacaagaaa aaaaagttca ggagctgcaa caagaaacag
    82501 ctcgtttagc cgaagaacaa gaagttttgc gtacacgagg cgaacaatta cgttttgaag
    82561 aacaagaagc gacaaatcag ctacaaaaca tcataaatga acttgaacgt tttgaaaaag
    82621 aaaagcaaat ctcaactttt gagacacgtg aactgcagca gtttatcgaa gattacgaaa
    82681 agcaaacgaa cgaattgaaa gacaaacaaa cagatcttga atcgcaacga caaaaaatcg
    82741 acgaagaaat caaatcgtta agtcaagaaa gcgaccagat ggaagccaga agagcacaag
    82801 tacaaagtca aaaagcacaa gaacaagcag atcttgctgt cttgaaagag cagtttaatc
    82861 atctgcagat ccagctccgc ggtgcccgag tccaaaaagc agaggcaacc gaacgtcaag
    82921 aagcgataga aaagcagttg gcgacattga cagcagactt ttctgatcat gaagtcacag
    82981 aagaaagttt ggaaaaacaa attaacgaat tatctgctca gcgagaaaca ttaaaagccg
    83041 agttggcaaa agccaaagag cagcgagata gaaggcaaaa ggagatcgat aagcttgaag
    83101 cagtcttggc agagcgtaat cgtgagcaaa aagcacgact gtcggaacaa tccaaattag
    83161 aagtccaaaa agaccgagct gaaatgattt tagataatca tttgacctat ctgcagtcag
    83221 aatatcagct gacatttgag aaagcttccc aagactatca agaaacaacc gatatcgaag
    83281 acagtagaac aaaggtttca tccttaaaag aacagattga aaaactggga ccagtaaact
    83341 taaattcaat cgaacaatac gaacaagtca gtgaaagaca tacattctta gcaacacagc
    83401 gagatgatct gttagcagcg aaaaaccaat tgtttgaaac gatggatgag atggatgatg
    83461 aagtgcggac aagattcaaa gaagtatttg aagctatccg tcaagaattc aaaattgtct
    83521 ttccaaatat gtttggcggc ggtaaagctg aattagtact gacggaacca actgatttgc
    83581 tgaaaacagg tattgagatt gaagtacaac ctcctggcaa aaaactgcaa agtctaagtt
    83641 tgctttctgg aggagaacga gcacttacag ccatcgcgtt gctcttttcc attatccgtg
    83701 tctgtcctgt tccgttttgt atcttagatg aggtagaagc agcgttagat gaagccaacg
    83761 tgaaacgttt tggtcgttac ttgagcgatt tccaagatga cacacaattt atcgtagtca
    83821 cacaccgcaa aggaacgatg gtcgcagcag atgtactgta cggagtgacg atgcaggaat
    83881 ccggtgtatc aaaaatcgtt tctgtacgga tggaagatat caatgaagag gcgaaaatca
    83941 acgaaacgaa agtgtaggaa cgaaaatgat caaattagta gcaatcgatt tggacggtac
    84001 attattaaat gaacaaaagc tgatttcaga tgagaataaa caagcattag cacaagcaaa
    84061 acagcaaggg gtcaagatcg ttctatgtac aggacgcccc ttagctgcga tggcgcatta
    84121 tctgcaagaa ttaggactgg tagatgaagg tgactacagc atcactttca acggcggttt
    84181 ggtacaaaaa aatgatactg gtgaaatcat ggaaaagaaa gtgatggaag tatcagatat
    84241 ccaacgctta tacaaactgg ctcagcaatt ggatctgcca ctagatgttt tgtctgacaa
    84301 tgtggtttta cagctgccta gtgccccaag gaagcaatcc ttgtacaatg tgttgaataa
    84361 gctgctgcaa ttccaaccag cggatataag cgatattaca gatgaatttg tgcttaataa
    84421 ggcagtaatc gcttatgacc aagaagaatt agatcctaaa atcaaagaaa taccagcaga
    84481 attccatgaa atgtatgaga tcatcaaaac aagaagtatg cttttggaat ttatgccaaa
    84541 aggtgttaca aaagcttatg gtatttcttt attggcaaaa gatcttggtt tgaaacaaga
    84601 agaaatcatg gcgatcggtg atgaggaaaa tgatttaccg atgatccaat atgctggact
    84661 aggcgtagcg atggagaatg ctgtgccttt cgtcaaagaa gcagcagatt tcgtgacagc
    84721 cagcaatgaa gaaaatggcg tagccaaagt gatccaaaaa tttgtattag agtaggggga
    84781 gtcaaatggg attttttgat aaaatcaagc gtgcttttac tggcgaagag aaaacagaag
    84841 aagttgaagt acaggagaag tacgacaagg gtttagaaaa gacgcgtaaa acatttggtg
    84901 aacggatgaa tgagttgttt gccaattttc gaacagtcga tgaagacttt tttgaagaat
    84961 tagaagaaac acttatcggt gcagatgtag gttttgaaac agcgatacga attaccgaag
    85021 cattacgtca agaagtgaaa ctcagaaatg cgaaaaaatc ggcagaagtc caaaatgcga
    85081 tcatcgagaa acttgtggat ctctatgaag cggaaggaat caatgaagta aatgaactaa
    85141 atatccaaga agacggacta acagttattt tgtttgtcgg tgttaatggt gtagggaaaa
    85201 caacaagtat tggtaaactt gcccatcagt tcaaaaacga aggtaaaaaa gtattgatgg
    85261 cagcagcaga taccttccgc gctggagcaa tcgaccagtt agttgtctgg ggcgaacgcg
    85321 ctggtgttga agttgttcgt ggcaatgcag gaggcgatcc agcagctgtc gtattcgatg
    85381 ctttggaaag agcaaagaca gagcaggcag atgttttact agttgatact gcaggccgac
    85441 tgcaaaataa agtgaacttg atgaacgaat tagaaaaaat aaaacgagta atccagcggg
    85501 aattaccaga agcaccccaa gaaacacttc ttgtattaga tgcaaccact ggtcaaaacg
    85561 cgatggttca agcaaaacaa ttcaaagaaa caacagatgt gaccggtctt gttttgacaa
    85621 aattagatgg tacagcaaag ggcggtatcg tacttgctat ccgtaatgaa ttgcatttac
    85681 cagtgaaatt ggtcggtctt ggtgaaggaa tcgatgactt ggagccgttt gatccaaatg
    85741 attttgtcgt tggattgttc aaaggattac taaaagaaga ataacccatg tctaaaaagc
    85801 aggagctgtg acaaaagttg cagctccttg tttgaatgat tgtttttaac atagctgtgt
    85861 ttttgatgcc ttcattttta tacttatttg taagcggttc tcaaacaatt tcgttctttt
    85921 tcttagactt tttgattata attagattca tatgaattgg aggtcactta atgatgaatt
    85981 ttaagcaatt attattgcat gtcaatgcgc gcccttttat cgaccaagct cgatttggta
    86041 tagaacggga aggacagcgg gttgatcttg caggaaatct agcaaaaacc gatcatccag
    86101 caatctttgg cgatcgatcc tatcacccct atatccaaac agattttagc gaaacacaaa
    86161 cagagatgat cacccctgtt accgattcta ttcccgaatt atttcagtat ctcgctgctg
    86221 tttatgatgt gactgctcgt tctataccga aagaagagat gatctggcca ttaagtatgc
    86281 cacctgcctt accggaaaaa gacgaagaga tcattattgc aaaattaaaa aatttcgaag
    86341 atgtcttgta tcgacgttat ttagcaaaag aatacgggaa gcgaaaacag atggtaagcg
    86401 gtattcattt caattttgaa tttggcgatg agttgctaag aacattgttc agccatcagg
    86461 aagaatttca agatttttcc gaattcaaaa cagaacttta tttgaaaaca gcgagaaact
    86521 ttatgcgcta tcgctggatg atcacttatt tattcggtgc ttcaccaatg agtgagaaga
    86581 actacttttt agatgaatca catccgcaag aacctgttcg cagtattcgc aacagtgcgc
    86641 tgggctatac gaatcatcca aatgtgaaag tttcctatgc ttctatgaaa cagtatttag
    86701 cagatatcga gcgaatgatc gaagaaggga aactttcgga ggagaaagaa ttttatacac
    86761 cgcttcgttt ccgaggcgga aagaaagtcg cagatttagc aacaacaggt gttcgttata
    86821 ttgaattgcg gaatatcgat ttgaatcctt atgcaagatt gggtatcaat ccagagcaag
    86881 ttcggttctt acaattattc ctgatgtaca tgttatggac agaagaaaaa gaagactgtg
    86941 accaatgggt agcagaaggt acgactcgaa ataacaaagt agcactagaa caaccatcag
    87001 atcaaacaga atttcatcaa gaaggtagag aaattcttga aggaatgaag caaatgctgg
    87061 ttgaattgga ttggttggat tctctttatt tagtagagga agcattgact caaatggatc
    87121 atcctgaaca aacgttagca gcgaaactct atcaagaagc acagctatcg agtcagcaag
    87181 aagttgctgt ggcattggga catcaatatt ataaagaaag ccatgaacgt ccctatcagt
    87241 tggccggttt tcgcgaaatg gaactttcta ctcagatatt tatgttcgat gccatccaaa
    87301 aaggtgtcca agtcaaagta ttagatgagt cggatcaatt tttgcgtctg caattccaag
    87361 accatgtaga atatgtgaaa aatgcaaaca tgacaagtaa ggacagctat atcgtcccac
    87421 ttatcatgga aaacaaaaca gtaacgaaaa aagtcttaaa agaagctggg ttccgggtac
    87481 caggaggcgc agaattttca tccatggaag aagcggtaaa agcttatccg agatttgccg
    87541 agcaggcatt tgtgatcaaa cctaaatcaa ccaattacgg gttaggtatc acgattttca
    87601 aggaaggagc tagtctggaa gattatcaag caggtttagc cattgctttt cgtgaagaca
    87661 gttcggtttt agtagaagaa tttatgccgg gaacggaata tcgtttcttt gtgatcgacg
    87721 gagaagtcca agccatcatg ttgcgagtcc ctgctaatgt tattggtgac agtatccgaa
    87781 cagtgaaaga acttgttgaa gaaaaaaata gtgacccttt gcgtggaacg aatcatcgtg
    87841 cacctttaga attgatccaa ttaggtgaat tagaacaatt gatgttgaaa gaacaagggt
    87901 tgacgatcga atcagttccc caagccaatc aaattgttta tctaagggag aactcaaata
    87961 tcagtacagg tggtgattcg atcgatatga ctgatgaatt ttctgaagct tacaaaaaaa
    88021 tcgccgtttc tgcggtagaa gcactaggag ctaagattag tggtatcgat ttgattatac
    88081 cagataaaga aatcgatcct acaactgata aaaaagcata tgggatcatc gaagcaaatt
    88141 tcaatccagc catgcatatg catgtttatc cttttgcagg aaaaggcaga agattgacga
    88201 tgaatgtatt gaaattgtta tatcctgaag tattttaaaa gaaaaagctt ttgacagatt
    88261 gtcctatttg atgtcaatct gatcaaaagc tttttttgca tgtaaaaatt tatgtgtttt
    88321 ttcgttttaa aataaacgat cccagaagct ttttttcttt tttggttttt ctttttcagc
    88381 tggcgaagag ttttcttctg ggtatttttt ttcgatatca atcacaggtt catttactgc
    88441 ttcttttgct gttaagagca atccgaattc ctcaggattt gcagtgacgt aatcgttgac
    88501 tacagtgaaa cggctattgt ttttcgttgc tagctggata tattggtttt ggacagaaag
    88561 gggtagagcc ccattcaaga gtatggaagc atctggatgt tttttgagtt cttttaaaaa
    88621 gttttctttg ttttttggtt ggatcatttc agcaacagtc atactaagat agcagcgttc
    88681 cctgaatgtc cctaaatatt tatgctgttc ttcagggttc actaaaggtg taccataaag
    88741 tcccttatcc aaatggcttt ttacatcatc ttgtgccata aaaaataccc tccttcttct
    88801 ctttgtcttt cttacgatta tagcatattc ccaataatac ctaaaacata tgaaaaagcg
    88861 gttgcttttg ttcaacgatt cacttaaaat aagaatgaaa atcattctca attagaaagg
    88921 tggattggat gcgctacgga cgactagtag cattattagt tattttaagt agtatttcat
    88981 tgttcatcgg agtatcgaat gtacaattgt cagaactgct ccagttagat gggcaaagct
    89041 tacaaatctt gctagtgact cgtttgccac gaacgatttg tttgatcctt gtgggagcga
    89101 ccagtagtat ttgtgggttg atcatgcaac atttgaccca aaataaattt gtttcaccaa
    89161 cgactgcggg cacgatggat agtgcgcgtt taggtatttt agtggctatg attttcctac
    89221 ctggtgcatc cttgattgtc cgatccttga cagcattttg ctttgctttt gcaggaacat
    89281 tgcttttttt atcccttacg cgattgttcc tccagaaaaa tcaattgatg cttccgttga
    89341 ttggagtcat gttagggaat atcattggtt cagttgctac tttttttgct tatcaattcc
    89401 agttggtaca aaatatgact tcatggcttc aagggaattt tgcgactgtg atacgaggaa
    89461 attatgagtt attgtatgct gttgttccat tgttgctctt ggtttggcta tttgcttacc
    89521 gatttacgat cattggtctg ggcgaatcgt ttgcagtaaa tctgggaata gattaccaga
    89581 agatgcagtt tttaggcatt ggcttagtat cattggctag tgcagtcatg ttgatcatgg
    89641 taggaagcat tccttttatc ggagtgatca ttccgaatct tgtctctaga atatatggag
    89701 atcaagtgca caaaacaatt ggcgtaactg ctttagcagg tagtttgttc ttagtatttt
    89761 gcgatcttgt cgctcgcgtg ttgatttttc cttatgaaat accagtcagt gtgatcgttg
    89821 gaatcatcgg tggggctatc ttcctttatc ttctgataag gggacgacga tatgcttaaa
    89881 aaaaagccat ctatttttat tggtatgctt gttcttgttt tatttgttac aggaatgtac
    89941 ttgacgatcc atacatatgg aaactggcat tttgcatgtg cattgaggag taagaaaatt
    90001 tttgcattta tccttgtctc gctagctgtc agcagtgcca caatcagctt tcagacaatt
    90061 acgcataacc agtttttgac tccttcagta ttgggacttg agtcgttata tgtccttgtc
    90121 caaacggtcc ttttcttctt tatcggagga gtgacgatgc ttcagcagac aggtacagtt
    90181 tttttcttat tgaacgttgg gctgatggtc gggctgagtc tattgttatt tttctttctt
    90241 ttgaaaaaaa tgagagcgaa tcttttttta ttactgatgt cagggatgat tttaggtacg
    90301 ctttttggta gtgtcagtac ctttttacaa gtcatcatgg atccgaatga atatgatttg
    90361 cttcaaggca gattatttgc aagctttggc aatatccaga gtcagtatct tttgcctgct
    90421 tctttgatca ttatcggctg ctggtacctg ttatttcgat atcgacatgt gctagacgtt
    90481 ttccatctcg gaaatgaaca agcagtaaac ctcgggatag acacagatcg ttttgaattt
    90541 ttgattttat gttgtgtcag tcttttgaca gggacatcaa cggctcttgt gggtccagtt
    90601 acttttttag ggtttattgt cgcaaatatc agttatcgtg tcttttctac ttatcgtcat
    90661 ggtcagctat ttatcggaag ctttctggta gctatgctgt ttctgacagg aggacagctc
    90721 gcagtagagc agattttcgg ctggaataca acagtgagtg tcgtcgttga atttgtcgga
    90781 ggaatttatt ttgtcacaaa attgattcgc gaaaggaaga gtatcctatg attgatttac
    90841 aaaaggtcaa taaactgtac catggaaaat cagtggttaa agatgtacag ttaacgattc
    90901 ctgaaaagaa attgactgct tttatcggtc caaatggtgc tggaaagtca acggtgttgt
    90961 cgatgatcag tcgtttgata ccaaaagata caggtgaaat ctatttagac cataacgaag
    91021 taaaggcttg gaaatccaat gaattagcca aaaaattatc gatcctacga caatcgaatg
    91081 aaataaatgt ccaaatcacc gtgaaagaat tagtagaatt tggacgattt ccttatacga
    91141 aaggaaaatt gacgacaaaa gacgaggaaa tcgttcagga atcactggaa caccttgggt
    91201 tagaagaaat agcggatcaa tcgattgaca ctctttcagg cggtcaactc caacgtgcgt
    91261 atattgcaat ggtgctagcg caagacacgg attatatttt actggatgaa cctttgaaca
    91321 atctagatat gaattttgcg gttcaaatca tgcagatttt aaaaaattta gtacaagaaa
    91381 tcgggaaaac aattgttatt gttcttcatg atatcaactt tgctgcaagt tatgcagacg
    91441 aaattgttgc aatgaaagat ggacagttat ttacgcaagg aacgacccaa gaaatgataa
    91501 aaaaagatgt attggatgaa ttatacgaaa tggacatacg gatttgtgag attgaaggcc
    91561 gcagattctg tctttatttc taaaactaac attaggagag aagagatgaa aaaattagta
    91621 gtaggtatct tgattctagc atcatttggt ttagcagcat gtggaaatag caacaacacc
    91681 agtcaggcag acacaaaaaa aagcagcaca caaactgaaa ctacattgac gataacagat
    91741 agtaatggag accaaatcga ggtgccaaat aatccagaaa aagtcgtcgt attcgacaat
    91801 ggttctttag atacgatgga tgctcttggt gttggagata aggtagttgg agcagctaca
    91861 agcagccttc ctgagtatct ttcatcctat aaaaaagtag aatcagcagg tggaataaaa
    91921 gaaccagact tagaaaaaat taatcagctt cagccagatc taatcatcat ttctggccga
    91981 caaagagatt ttcaaaaaga tctaagtcaa attgctccga ccattttcct ttctttagat
    92041 gcgaaaaatc catgggaatc tttccagcaa aatgtcactg ctttagggga gatctttggt
    92101 aagcaagaag aagctaaaac tcaattagag gaattatcta gtgcaatcga tcagacgaaa
    92161 aagaaagcag aagctactga caaaaaagca ctagtcacac ttgtgaatga agggcaatta
    92221 tcagcctacg gatctggttc tagattcggt ttcatccatg atttatttgg ctttgaacaa
    92281 gcagatgatc aaatcgaagc atccactcac ggtcaaagtg tttcttatga atatgtgctg
    92341 gaaaaaaatc cggatatctt atttgtagta gacagaacaa aagctattgg tggagacgac
    92401 agcaaagatg atatttctgc caatgaactg gtagcacaaa cgaatgctgg taaaaatcag
    92461 cagatcatct cccttgaacc agatgtctgg tatttaagcg ggggcggttt agaatcaatg
    92521 aaactgatga tcgaagatgt gaatcaagca tttaaatagc ataaataaca gaggattaaa
    92581 ctctcatact atgtaacaat aatgtttctt acattcttaa atagcaagag cctatcctca
    92641 aagaagttat caaaaaccat ccgtaaaatc gtattgatga ttttacggat ggtttttatt
    92701 tatttgagaa atcttttttc aacgtgaatt cattatctac atcatacggg ttgattctag
    92761 aatggggagt ggcaaaaaat agtgtgatcc attttttcag catcaagatc gcctcctttg
    92821 atttgaatca aaggtacaaa gcgaaactta gataaaactt aaatttagat atttttattt
    92881 tgtaccgatc tcttctttaa acgatgttcc atctgctgct tctcggcaat aagtccacta
    92941 gactagaaaa tatgagaagc tattttctgt ccagcgttct tattactcaa agcaaatcgc
    93001 agttgtcaca atctcttcta aacgatgttc catctgctgc ttctcggcaa taagcccact
    93061 agactagaaa atatgagaag ctattttctg tccagcgttc ttattactca aagcaaatcg
    93121 cagttgtcac aatctcttct aaacaatgtt caatcagctg accttcggta ttagctcaaa
    93181 gcactgattt cacaacctct atttaggcat gtgtctcagc aagcggttga tagttgatga
    93241 tctgcatttt tcgataaaaa tcggcgggaa gcttactcca ccgttttaat aattgataga
    93301 aataactctc ttctgctttg attttatact cgcttaatat caatccaaaa ctattcgttt
    93361 tttttgaagc taatttttta taaatatcca atgctttggt tttatcttca ttgacaaaga
    93421 gtgtttttac ttcaatataa gaaccctcga tattttgtgt ttcttcgata aaatagatgt
    93481 tttttatata ttccttcata gtgattcctc caaagaagtc ttttatttga taagtttact
    93541 ctaccgaaga gttgttaata atccgtgaac aaaaagtaat tctttttcca atagttgtta
    93601 aaggtttttt attttttata gtattttttg ttatttaaga aaggaataag gttaaacaaa
    93661 taacaggttg ctttttgggt ctttttccgt tcaagaaact gaacgaagaa aagggaaagt
    93721 tgtctctttt gtgataagag aaatcataat aaacgaatga cttactttcc aataaattga
    93781 gaacagtcag aaccagacaa attagttgca tctgatgaat aaagtgatta ttctatgaca
    93841 tgaggtgata cagatgaaat tagaagtgac aaaagaagca caagaagtat tgaagaataa
    93901 attagaagca gacgatcaac ttttattaga tatcgaaaac ggtgacggtc catttgcaga
    93961 aagccaagtc agttgccaat tggatacggc atttcgattg atcattgtca aaaaagatac
    94021 acaaacagat ctaagtcttt attctgtagt ggctgataca ccagttggac caatcaaaat
    94081 caaagacagt gcaatacttt acatggatga tccatccaca cttgctttag aaccaactta
    94141 taatagttta caattaaaag gaccaagcgg cttgagaaaa ggcaacttgc aagttgttcg
    94201 aaaagactag agggataaaa tgggaaaatt ggcgatcatc agcgacctac atgtggacat
    94261 caataaattc ggcgaatcag agctgatgca gctttggcaa gttttgcaaa agcacaagat
    94321 cactcggctt catctagccg gagatacggc taataaagta gaccgttgcc tagctgttgt
    94381 cgaatttttc gtcaataaag gattatctac gacatttcat ttcggcaatc acgagttggc
    94441 agatgtgccc aatgaacaga tgatggacca ttttccagac ccacattttt taaatgaacg
    94501 gtatattgct ttaaatgagc agaccgttct tttagggatg aatggctggt acgactatca
    94561 gttttccgaa ttgaaagatt acgaggcaat cgtacgaatg aagcgtttgt attggtatga
    94621 tcggttcatc cagcgtggga aaacagatct cgaggtcaat gcagaaatgc ttgcagctgc
    94681 aaaaaatttg ctagaccagc tggaagcaaa aaaacttgag gtgattttat ccactcattt
    94741 cgttcctaaa cgggcattta tcatgtatca atctgctcct tatgagcgtt ggaacaaatt
    94801 aaatgcattt ttaggatcag aatcattcgg agaattactt gaccgttatt caaatatcaa
    94861 acaagtagtt tttggccata cgcatcgtcg ctttgaagat aaatccatcg gtcacacgat
    94921 ttacagctgc agaccttttg gttattatta tgaatggcag ctgacccgag attttgtctt
    94981 gaaagaacag ttgctagaca gctatgatcc tatgaaatta cgtgtcctct tgcgtgccca
    95041 tcacccagct tttcaggcct atcaaacaca acatttgcaa gaggaatttg aacaagcgat
    95101 gacaatcgtt ccgtattgag acagcattag taatgtgtaa aagaaaatac cttgtaaaac
    95161 ataaggataa gtaagagcag tagatccatt tatcgtggat atgctgcttt ttttgcggaa
    95221 aataatcagc gaatgtttat ttgatcggat gatttgtaac tcgcagagta ctagatgcaa
    95281 gcgtgtattc tttttttgaa cgataatcac aaaaaaaaat ata
//
LOCUS       DAYYFU010000005        84917 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000005 DAYYFU010000000
VERSION     DAYYFU010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 84917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.005"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1589)
                     /locus_tag="ACHA34_000429"
     CDS             complement(<1..1589)
                     /locus_tag="ACHA34_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8791107.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKI"
     gene            complement(1604..5125)
                     /gene="mfd"
                     /locus_tag="ACHA34_000430"
     CDS             complement(1604..5125)
                     /gene="mfd"
                     /locus_tag="ACHA34_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFM8791108.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(5142..5702)
                     /gene="pth"
                     /locus_tag="ACHA34_000431"
     CDS             complement(5142..5702)
                     /gene="pth"
                     /locus_tag="ACHA34_000431"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFM8791109.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            6055..7029
                     /locus_tag="ACHA34_000432"
     CDS             6055..7029
                     /locus_tag="ACHA34_000432"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8791110.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            7199..8599
                     /locus_tag="ACHA34_000433"
     CDS             7199..8599
                     /locus_tag="ACHA34_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFM8791111.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(8677..9399)
                     /locus_tag="ACHA34_000434"
     CDS             complement(8677..9399)
                     /locus_tag="ACHA34_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8791112.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(9478..9984)
                     /locus_tag="ACHA34_000435"
     CDS             complement(9478..9984)
                     /locus_tag="ACHA34_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8791113.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            10107..11906
                     /locus_tag="ACHA34_000436"
     CDS             10107..11906
                     /locus_tag="ACHA34_000436"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFM8791114.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(11974..13548)
                     /locus_tag="ACHA34_000437"
     CDS             complement(11974..13548)
                     /locus_tag="ACHA34_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFM8791115.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(13803..14312)
                     /gene="mreD"
                     /locus_tag="ACHA34_000438"
     CDS             complement(13803..14312)
                     /gene="mreD"
                     /locus_tag="ACHA34_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFM8791116.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(14316..15170)
                     /gene="mreC"
                     /locus_tag="ACHA34_000439"
     CDS             complement(14316..15170)
                     /gene="mreC"
                     /locus_tag="ACHA34_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFM8791117.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(15325..15963)
                     /locus_tag="ACHA34_000440"
     CDS             complement(15325..15963)
                     /locus_tag="ACHA34_000440"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFM8791118.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(15964..16614)
                     /gene="rpe"
                     /locus_tag="ACHA34_000441"
     CDS             complement(15964..16614)
                     /gene="rpe"
                     /locus_tag="ACHA34_000441"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFM8791119.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(16627..17526)
                     /gene="rsgA"
                     /locus_tag="ACHA34_000442"
     CDS             complement(16627..17526)
                     /gene="rsgA"
                     /locus_tag="ACHA34_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFM8791120.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(17691..19760)
                     /gene="pknB"
                     /locus_tag="ACHA34_000443"
     CDS             complement(17691..19760)
                     /gene="pknB"
                     /locus_tag="ACHA34_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFM8791121.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(19757..20497)
                     /locus_tag="ACHA34_000444"
     CDS             complement(19757..20497)
                     /locus_tag="ACHA34_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFM8791122.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(20510..21868)
                     /gene="rsmB"
                     /locus_tag="ACHA34_000445"
     CDS             complement(20510..21868)
                     /gene="rsmB"
                     /locus_tag="ACHA34_000445"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFM8791123.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(21868..22806)
                     /gene="fmt"
                     /locus_tag="ACHA34_000446"
     CDS             complement(21868..22806)
                     /gene="fmt"
                     /locus_tag="ACHA34_000446"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFM8791124.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(22799..23290)
                     /gene="def"
                     /locus_tag="ACHA34_000447"
     CDS             complement(22799..23290)
                     /gene="def"
                     /locus_tag="ACHA34_000447"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8791125.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(23305..25713)
                     /gene="priA"
                     /locus_tag="ACHA34_000448"
     CDS             complement(23305..25713)
                     /gene="priA"
                     /locus_tag="ACHA34_000448"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFM8791126.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(25966..26271)
                     /gene="rpoZ"
                     /locus_tag="ACHA34_000449"
     CDS             complement(25966..26271)
                     /gene="rpoZ"
                     /locus_tag="ACHA34_000449"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8791127.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(26276..26890)
                     /gene="gmk"
                     /locus_tag="ACHA34_000450"
     CDS             complement(26276..26890)
                     /gene="gmk"
                     /locus_tag="ACHA34_000450"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8791128.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(27377..27661)
                     /locus_tag="ACHA34_000451"
     CDS             complement(27377..27661)
                     /locus_tag="ACHA34_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791129.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(27679..28569)
                     /locus_tag="ACHA34_000452"
     CDS             complement(27679..28569)
                     /locus_tag="ACHA34_000452"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFM8791130.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(28589..28807)
                     /locus_tag="ACHA34_000453"
     CDS             complement(28589..28807)
                     /locus_tag="ACHA34_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFM8791131.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(28777..30477)
                     /locus_tag="ACHA34_000454"
     CDS             complement(28777..30477)
                     /locus_tag="ACHA34_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFM8791132.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            30744..31517
                     /locus_tag="ACHA34_000455"
     CDS             30744..31517
                     /locus_tag="ACHA34_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791133.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(31598..32167)
                     /gene="thiT"
                     /locus_tag="ACHA34_000456"
     CDS             complement(31598..32167)
                     /gene="thiT"
                     /locus_tag="ACHA34_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFM8791134.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(32233..32335)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(32341..33312)
                     /locus_tag="ACHA34_000457"
     CDS             complement(32341..33312)
                     /locus_tag="ACHA34_000457"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8791135.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(33406..34779)
                     /gene="glmU"
                     /locus_tag="ACHA34_000458"
     CDS             complement(33406..34779)
                     /gene="glmU"
                     /locus_tag="ACHA34_000458"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFM8791136.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(35282..36100)
                     /gene="purR"
                     /locus_tag="ACHA34_000459"
     CDS             complement(35282..36100)
                     /gene="purR"
                     /locus_tag="ACHA34_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFM8791137.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(36267..37091)
                     /locus_tag="ACHA34_000460"
     CDS             complement(36267..37091)
                     /locus_tag="ACHA34_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM8791138.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(37064..37744)
                     /locus_tag="ACHA34_000461"
     CDS             complement(37064..37744)
                     /locus_tag="ACHA34_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8791139.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(37767..38705)
                     /locus_tag="ACHA34_000462"
     CDS             complement(37767..38705)
                     /locus_tag="ACHA34_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM8791140.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(38950..39801)
                     /gene="ispE"
                     /locus_tag="ACHA34_000463"
     CDS             complement(38950..39801)
                     /gene="ispE"
                     /locus_tag="ACHA34_000463"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFM8791141.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(39955..42528)
                     /gene="mprF"
                     /locus_tag="ACHA34_000464"
     CDS             complement(39955..42528)
                     /gene="mprF"
                     /locus_tag="ACHA34_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8791142.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            42669..43475
                     /gene="proC"
                     /locus_tag="ACHA34_000465"
     CDS             42669..43475
                     /gene="proC"
                     /locus_tag="ACHA34_000465"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFM8791143.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(43554..45110)
                     /gene="rny"
                     /locus_tag="ACHA34_000466"
     CDS             complement(43554..45110)
                     /gene="rny"
                     /locus_tag="ACHA34_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFM8791144.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(45521..46570)
                     /gene="recA"
                     /locus_tag="ACHA34_000467"
     CDS             complement(45521..46570)
                     /gene="recA"
                     /locus_tag="ACHA34_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFM8791145.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(46679..47920)
                     /locus_tag="ACHA34_000468"
     CDS             complement(46679..47920)
                     /locus_tag="ACHA34_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFM8791146.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(48160..48738)
                     /gene="pgsA"
                     /locus_tag="ACHA34_000469"
     CDS             complement(48160..48738)
                     /gene="pgsA"
                     /locus_tag="ACHA34_000469"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFM8791147.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(48947..49870)
                     /locus_tag="ACHA34_000470"
     CDS             complement(48947..49870)
                     /locus_tag="ACHA34_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFM8791148.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(50609..51895)
                     /locus_tag="ACHA34_000471"
     CDS             complement(50609..51895)
                     /locus_tag="ACHA34_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8791149.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(51888..53156)
                     /locus_tag="ACHA34_000472"
     CDS             complement(51888..53156)
                     /locus_tag="ACHA34_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8791150.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            53408..53869
                     /gene="mscL"
                     /locus_tag="ACHA34_000473"
     CDS             53408..53869
                     /gene="mscL"
                     /locus_tag="ACHA34_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFM8791151.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(53929..54375)
                     /locus_tag="ACHA34_000474"
     CDS             complement(53929..54375)
                     /locus_tag="ACHA34_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8791152.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(54468..54713)
                     /locus_tag="ACHA34_000475"
     CDS             complement(54468..54713)
                     /locus_tag="ACHA34_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFM8791153.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(54828..55406)
                     /locus_tag="ACHA34_000476"
     CDS             complement(54828..55406)
                     /locus_tag="ACHA34_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8791154.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(55483..56013)
                     /locus_tag="ACHA34_000477"
     CDS             complement(55483..56013)
                     /locus_tag="ACHA34_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFM8791155.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(56017..56877)
                     /gene="rlmB"
                     /locus_tag="ACHA34_000478"
     CDS             complement(56017..56877)
                     /gene="rlmB"
                     /locus_tag="ACHA34_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFM8791156.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(56870..57265)
                     /locus_tag="ACHA34_000479"
     CDS             complement(56870..57265)
                     /locus_tag="ACHA34_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFM8791157.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(57262..58674)
                     /gene="cysS"
                     /locus_tag="ACHA34_000480"
     CDS             complement(57262..58674)
                     /gene="cysS"
                     /locus_tag="ACHA34_000480"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFM8791158.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(58671..59207)
                     /gene="epsC"
                     /locus_tag="ACHA34_000481"
     CDS             complement(58671..59207)
                     /gene="epsC"
                     /locus_tag="ACHA34_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFM8791159.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(59293..59532)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(59529..60989)
                     /gene="gltX"
                     /locus_tag="ACHA34_000482"
     CDS             complement(59529..60989)
                     /gene="gltX"
                     /locus_tag="ACHA34_000482"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFM8791160.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(61159..62316)
                     /locus_tag="ACHA34_000483"
     CDS             complement(61159..62316)
                     /locus_tag="ACHA34_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFM8791161.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(62350..63720)
                     /gene="radA"
                     /locus_tag="ACHA34_000484"
     CDS             complement(62350..63720)
                     /gene="radA"
                     /locus_tag="ACHA34_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFM8791162.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(63783..64310)
                     /locus_tag="ACHA34_000485"
     CDS             complement(63783..64310)
                     /locus_tag="ACHA34_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFM8791163.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            64540..65346
                     /gene="proB"
                     /locus_tag="ACHA34_000486"
     CDS             64540..65346
                     /gene="proB"
                     /locus_tag="ACHA34_000486"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFM8791164.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            65343..66590
                     /locus_tag="ACHA34_000487"
     CDS             65343..66590
                     /locus_tag="ACHA34_000487"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFM8791165.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            66666..67010
                     /locus_tag="ACHA34_000488"
     CDS             66666..67010
                     /locus_tag="ACHA34_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791166.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(67127..67453)
                     /locus_tag="ACHA34_000489"
     CDS             complement(67127..67453)
                     /locus_tag="ACHA34_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791167.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(68068..69270)
                     /locus_tag="ACHA34_000490"
     CDS             complement(68068..69270)
                     /locus_tag="ACHA34_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8791168.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            69387..69902
                     /locus_tag="ACHA34_000491"
     CDS             69387..69902
                     /locus_tag="ACHA34_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8791169.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(70101..70547)
                     /locus_tag="ACHA34_000492"
     CDS             complement(70101..70547)
                     /locus_tag="ACHA34_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8791170.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(70594..72072)
                     /gene="msr(C)"
                     /locus_tag="ACHA34_000493"
     CDS             complement(70594..72072)
                     /gene="msr(C)"
                     /locus_tag="ACHA34_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8791171.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(72426..73055)
                     /locus_tag="ACHA34_000494"
     CDS             complement(72426..73055)
                     /locus_tag="ACHA34_000494"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8791172.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(73039..73503)
                     /locus_tag="ACHA34_000495"
     CDS             complement(73039..73503)
                     /locus_tag="ACHA34_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8791173.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(73990..75258)
                     /locus_tag="ACHA34_000496"
     CDS             complement(73990..75258)
                     /locus_tag="ACHA34_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8791174.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(75489..76226)
                     /locus_tag="ACHA34_000497"
     CDS             complement(75489..76226)
                     /locus_tag="ACHA34_000497"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8791175.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(76293..76862)
                     /locus_tag="ACHA34_000498"
     CDS             complement(76293..76862)
                     /locus_tag="ACHA34_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM8791176.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            76990..77862
                     /locus_tag="ACHA34_000499"
     CDS             76990..77862
                     /locus_tag="ACHA34_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8791177.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            78204..79076
                     /locus_tag="ACHA34_000500"
     CDS             78204..79076
                     /locus_tag="ACHA34_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8791178.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            79073..81031
                     /gene="nagE"
                     /locus_tag="ACHA34_000501"
     CDS             79073..81031
                     /gene="nagE"
                     /locus_tag="ACHA34_000501"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM8791179.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(81363..82214)
                     /locus_tag="ACHA34_000502"
     CDS             complement(81363..82214)
                     /locus_tag="ACHA34_000502"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8791180.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            82337..82804
                     /locus_tag="ACHA34_000503"
     CDS             82337..82804
                     /locus_tag="ACHA34_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFM8791181.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(83067..83549)
                     /locus_tag="ACHA34_000504"
     CDS             complement(83067..83549)
                     /locus_tag="ACHA34_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8791182.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(83649..84512)
                     /locus_tag="ACHA34_000505"
     CDS             complement(83649..84512)
                     /locus_tag="ACHA34_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8791183.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        23984 a        18210 c        15313 g        27410 t
ORIGIN      
        1 ttaatttttt tatttaatcg caggattttc ttaccaaaag gcaacattag ccattctcgt
       61 actgtaaaca gacgaagacg aatcactgca taaatgaaca cagaagcccc gatcccaact
      121 cctatcacgc tgacaaaaaa agcagtggaa cgatgagtta caggaccaaa tactatattc
      181 aatacaccat aatacagcag cagtactact gtcatacaaa gaaggctgaa catcaacttc
      241 cgtccaaaat gccgttcttt ccaaaaagca tttagctctt ttgtttcaat tcgaacaaaa
      301 taccaaagcg tcacacctaa tcctaaaagg gtagacaggc ttgcacctgc tgttcctaat
      361 aaaccggtca gaaaagaagt agtcaatcct tttaccaata gtccccatcc tgcgcctttt
      421 aaagaagggc gaaacgagtt cttgctttga gcaatacttt gataagcttg gatgactgca
      481 gtgaaagcaa tggaaaaaac aaataataca agcgctgcat ttcccgcata atctttaaac
      541 aacgcataat tgatataagg gagcagcaag gctaatccca aagacgcagc taatgctaaa
      601 gcggtcgtca atctaagata gattttggct gtctgtaaaa actgacgata gatccggttg
      661 gtcaaatgac gagtcaacgc aggcaggaat gtcgcactta atgcagtggc aatcactaaa
      721 cctaattgta ctaaaggctg acctcgatca tagacccctt ttgctatctt cgcaccgtat
      781 tctgacagtc cgtaaacttc caaagcattt tttacaaaga aagagtcgat cagctggaaa
      841 aagataagca atccactata aatcgaaacc agtccgcctt ctaccagaaa acgccggatc
      901 aagggctttg acggctgttt gaacaatgga aaccgtcgga agcttaacgc tcctccatga
      961 atcttttgtt catagtagtg aaggatccca tatgcgcata aaccgccaaa aacagctcca
     1021 ctcattgctg ccgtccctgt ttggtacacc gtccatccaa gctttctaaa agcaaacgca
     1081 ctacagacaa tcacaaaaac acgaacgaac tgctcaacta cttgagaaac agcagttggt
     1141 tccatcaaga atcttccttg gaaattccct cgataaaaag atagaccagg catcagcaaa
     1201 aaggtaaagg aaactacttg gatcagcggt ttcaattgta tgtctcccat catccaagca
     1261 atccagctgc taaatgcaaa gacaaacaac cacaaaaaga cacctgtcca aaatactaag
     1321 ggataaagat tttgcaaagc ttgctttcgc tcgaaaggat tctttctttc tgcaacgtac
     1381 tttgatatga actgcggaag accggataaa gctaaagtca ttgctatccc ataaattgga
     1441 taaacttgct gataaacata aaaaccttcg tcgccgacta agttttggag gggaattcgg
     1501 taaagggcac ttaatacttt tgctatgaaa gaagcaatcg tcaatacaaa tgctccttgc
     1561 atgacgcgcc gcatttcttt atgatccaaa tcattgctcc ttcctacgct gacagatatt
     1621 tttgctcacg caacgctttg acaaactttt ggatttcctg aagccatact gcttcttcca
     1681 tttggttggg gatcgtcaag cgtatcacca tctgttcttt ttcaacgcct agactggctt
     1741 ttagttttgt cgctgtcagt gcttcgaata actgttccac actatagcgt tttgttccaa
     1801 ctttacttag agtaaagact atatgcgcct cctgtttcct tatcgtttct aggagcgctc
     1861 gatcgccatc cattttgatc tgcccaattg tcaagagatg tgctacttct tctggatatt
     1921 ctccaaaacg gtcgagcaga tcatcttcta gttcatctag catttcttgc gaatcaagtt
     1981 cacgaattcg tttatagatc tcgattttct gccgttgatc tgccacgtat gtttcaggta
     2041 gatacgcatc gacaccgagg tcgatctcaa cagtggtctt ctccacttga ctattctttc
     2101 cttgtttacg agaaacagct tcgcttagca tttgagaata catatcgaaa ccgactgcat
     2161 caataaatcc atgctgctga gcacctaaga gattacctgc accgcgaatg gaaagatccc
     2221 gcatcgcaat cttgaatcca gatcccagtt ctgtaaaatc tttgattgcc tgaagacgtt
     2281 tttcactgac ttcgttaagg atcttctgct gctcatacat aaagtaagca tatgcgacac
     2341 ggctgcttct tcctacccgc cctcgcaatt ggtataacgt ggataatccc atataatcag
     2401 cgttctcaac gaacaacgta tttgcgttcg ggatatctac tcctgtttca atgatggttg
     2461 tagtgacaag aacatcatat tgaccttcaa taaattcaaa aagcgtattt tctaattgcg
     2521 cctctgtcat ttgcccatga gcataaccga tccgtgcttc cggaacgagt tcttggattt
     2581 cttctacttt ttgttcgatc gtttctaccc gattatataa atagaatact tgtccacctc
     2641 gtcccatttc tcgatggatt gcttcgcgaa tagccccagg atttttttcc ataacatacg
     2701 tttggatcgg ataacgattc gcaggcggtg tttcgattac tgaaagatcc cgcacgccta
     2761 acatcgacat atgcaaagtt ctcggaatcg gtgttgcagt cagagtcaaa acatccactt
     2821 gtgcacgtaa ctgtttcaat cgttccttat gttttacacc aaaacgttgt tcttcgtcaa
     2881 tgatcagtag acccaaatcg ctaaattcaa tatcttttga caaaattcgg tgggttccta
     2941 caacaatatc aacttgacct gtacgcagtt gttcgatggt ttctttttgt tgctttcttg
     3001 ttctgaaacg acttaatagc ccgatattta cggggaaacc ttcaaatctt tccagcatcg
     3061 tttcatagtg ctgctgtgcc aagatcgttg ttggaactaa aaaggctact tgtttgcttt
     3121 cctttatcgc tttaaaggcc gctcgaagcg ctacttctgt tttcccataa ccaacatcac
     3181 ctacgagtag tcgatccatt ggtttttctt tttccatatc ccgtttgatc tccgctgcac
     3241 ttctcaactg gtcatcagtt tcagagtagg ggaaggcatt ttcgaattct ttttggtagg
     3301 cgtcatcagg tccaaaagca tatccttttt ctgactctct tgcggcatat aatttgatta
     3361 gatcgtcagc gatatcctcg atttttgaag agacttttcg cttggttttc gtccattcac
     3421 ttccgcctag cttattgatg cgaggcgctt tggcttcaga ggcgacatat ttttggatca
     3481 gattcagttg agtaacagga ataaacagtt tgtcgtcgtt ttgatacaaa atagtcatat
     3541 agtcttggtg aacgccatct acttctagcg tttccatccc aatgtatttc ccgataccat
     3601 gattggcatg gacaacgtaa tctcctgctt ttaactcatt atagcttttc aatcgctctg
     3661 cgtttgttac tgtttgacgt ctcgtttgtt ttttcgtcgt tttttggaaa atttcttttt
     3721 ctgtgatagc tactattttt tcttgaggaa gttcaaaacc agcctgcaaa gctccttcaa
     3781 ctaattgtac ctgtcctctg atcaatgcat ttttttctgt ttctactact aatatgtctt
     3841 catctcgaag catttgttct gctttgcgta ttctgtcctt tgtcggaata aagatcacaa
     3901 cagtctgttt ttgcttgcgc caacggtcca tttctgtttt cagcaatggc atttgtccga
     3961 agaactgttg catcgtccga tattgaaaat tatggattgc ttggaaacga agatttccca
     4021 tccctttttg gaagagtgag aaaaaggttg tggtgaactt ttccttctgc acgatttttt
     4081 gtatatctgc tgaaaatgtc tgctcagaaa acacccgcaa ttcgctgatt ttttgagtct
     4141 gccattctgc cgcttccctt tcgatttccc gatttgtttc catgattcgc tgataatcat
     4201 caacgactag taaactattt gaagggaaat aatccaagat cgtcacagat tcttggtaaa
     4261 gaagatctga gtagaatttt gcttcttctg tcgggacacc tgcttgccag cttgaagtca
     4321 gctgaccgaa gtaatcttgc aaaaactcgc ggtctgcttc ttctgtggtc gcagcaagcc
     4381 gtttttctag cattttttgc atttgctcta ttccatgttc tagatcctct ggtccgtaga
     4441 cttgttcact cgttggggaa acccagattt cttccgtttt ttcgattgag cgttgtgttt
     4501 ctgcttcaaa ataccgcata gaatctatct ctacatcaaa tagttctaca cgaacaggat
     4561 aggttgtcgt taacgggtaa atatcgataa tgcttcctcg aatactgaat tctcccggtt
     4621 ttgcgaccat ctcttctcgt acatagccca tcaaagtcaa ttggcgagga agattttcca
     4681 cgtctatctc atcaccaata tgccaattca attgcgcctg tagccaagtt ttcttattcg
     4741 gcagtcgttt gtgcaaagat gcgactggca acaaatagat acctgctttt ttttcttgaa
     4801 gtgcgttaag cgttgcaact ctctcgcctc gcgcttctgg agaagaaaag gccatttcag
     4861 cagaaagcac ttcatccact ggaaataaat ggatctgatc ttgcggaatc acatgttgca
     4921 gatcttctgc taactgattc gcataataca aatttggcat cagaataatc atgtttttat
     4981 ttgttttctt aaacgctcga gcaaatagaa gtgttttcgc tgagcccgct aatcctgtga
     5041 ttagttgtct aggcattttt tcttctaaat tgctgaccca ttcttttact tgcggatctt
     5101 ttccgactaa ttgaataata tccattcctt ttcctccttc ctcaattgaa tcgattcatc
     5161 gcatccacga acgaatgccc ttctatgaga tattcgactg ctttaacaga ttgacacatg
     5221 ctttcttcta tcaaaggctg attttctttg ctgaaaggac taagcacatg ctggactact
     5281 gtttttttcc cttctggcct gccgattccc accttgattc gatcaaagac attcgtattc
     5341 aaatgggaaa taatactttt gataccgtta tgcccgccgg cacttccctt ttgacggagc
     5401 cggatttttc ccactgccag atccaaatca tcgtagatta cgactaattc ctctggataa
     5461 atcccaaaat aagtcattag tgggccgact gcacgaccag attcattcat gaatgtttgt
     5521 ggtttgacta aaaggatctt ttctccatta tgaaaaaatt cggcaacttc tgcttcgaac
     5581 ggattttttt tgaaagtagc ttgatgctcg cgtgcaattt tatccacgac cataaagccg
     5641 atattgtgtt ttgtatattg gtatttggta cctgggtttc ctaacccgac gatcattttc
     5701 atttttgttt tccacctaat tctaaaaagc agcacaaaag gctggactgc taattagcgg
     5761 tccagtccat cgagcttttt tcttttttac acttgtcaat gcatagtata acacaaaaca
     5821 aggcgtttcg gctaaaagag aatcgttaat aaatgataaa gaaattaaaa atttcttcaa
     5881 agtttaatga aataattgat atataaaaat agtgaaataa cgcacatgta agcttttaca
     5941 tataatacaa ccttgttatc tgttatattt tatagccaaa tagcgtgaca tggggcttga
     6001 gaaatgttag cataagcatg ggaaaaaaca aatcaatgga aggattgata ctaaatgact
     6061 gcagctgcag aaaaaaaaga tcatcaaaaa gttattcttg ttggagacgg cgccgtaggt
     6121 tctagctatg cctttgctct agttactcaa aatatcgctc aagaagtagg tattgtggat
     6181 atcaatactg ctaaaactga aggagacgca attgacttgt ctcacgcttt ggcatttact
     6241 tcacctaaaa aaatctattc tgctacttac gcagacgcac atgatgctga cttagttgtt
     6301 attacagctg gcgcaccaca aaaaccaggt gaaactcgtt tagacttagt aaacaaaaac
     6361 ctaaaaatca accgcgatgt tgtcacacaa atcgttgaat caggatttaa cggtatcttt
     6421 ttagttgccg ctaacccagt agatattttg acttactcaa cttggaaatt ctcaggtttc
     6481 cctaaagaac gcgtaatcgg ttcaggtacc tctcttgact cagctcgttt ccgtcaagca
     6541 cttgcggaat tagttgacgt tgatgcacgt aacgtccatg cttacatctt aggtgaacac
     6601 ggagattcag aattcccagt ttggtcacat gcaaatgttg ccggcttgca aatctatgaa
     6661 tgggtaaaaa acaacccaga aatcgatgaa gaagctatgg ttaacctatt ctttggcgta
     6721 cgtgacgctg cttacacaat tatcgagaaa aaaggcgcta ctttctatgg tatcgcagta
     6781 gcattagcac gtatcactcg tgccatctta gatgatgaaa atgctgttct tcctttatca
     6841 gtattcatga atggcgaata tggattgaac gacatctaca ttggtgcacc tgctgttatc
     6901 aaccgtcaag gtatccaaaa agtaatcgaa attcctttaa gtgacagcga gcaagaccgt
     6961 atggctgctt ctgctaagca attaaaagaa attctagatg aagcttttgc taaactagac
     7021 gctgaataat ctttgaacat gacggaaaac atctattcgg tgttttccgt ttttttattt
     7081 tatcaccctt atggtttcta aattttttca taaagtagag ataaccttcg tcttttcctc
     7141 taaattatgc tatcatgaga ccgttccacc ctaaactttt acttaaggag ttgtcgatat
     7201 gacaagatct tcgcatcgaa aaaaatcgaa acatgttgct ttgctttcag ttcttggtgt
     7261 tctcttagtg ctgatcagct tttataccta tcgcagtaca tactacacaa agcatttttt
     7321 gccgaataca gaaataaatg gaataaatgt cagcaatcta acagtcgaaa aagcaaatga
     7381 aaaattaaaa gaagcttatt ctgataaaaa actggctatt aaagaagatg gaaaagtctg
     7441 gcaggaagta ccaaaatccg agctcggcta caaagatgat ttcacttcgg agctttctcc
     7501 gatcctaaat gaacaaaacg gatggacttg gggaatgact tatgtctcag cggcagaaaa
     7561 acaagaaata gatcctgtca gtcaaaacca ccaaaaacta gacactaccg ttcagacatt
     7621 gacaactaag ctgactgatt tgaacaaaga ccggacacct actactgatg caacgatcga
     7681 aaaagcaggt gactctttcg ttatcaagcc tgaagtcaat ggaaatacga tcgacgtcga
     7741 tgctgcggtc aaacaattaa aaagtgcggt aaactctggt aaagatacaa ttgaattgac
     7801 tgaattcaaa gaaaaaccaa aagtaacatc tgaagacagc tcactgaaag aacaactcgc
     7861 atccatgaat gctatcgcaa atgtaaaagc tacttacagt ataaacggtg agactttcca
     7921 gatcccatca agtgatatta tgagttggct gacctacaat gatggaaaag tcgatttaga
     7981 tacagaacaa gtacgtcaat atgtaacaga tttaggcaca aagtataaca caagtacgaa
     8041 tgacacaaaa tttaaaagta caaaaagagg agaagttacg gtcccagttg gcacttacag
     8101 ttggacgatc caaacagaca gtgagacaga agcattgaaa aaagcgattc tggcaggcca
     8161 agattttact cgttcaccca ttgtgcaagg tggcacaacc gctgatcatc cattgatcga
     8221 agatacgtat atcgaagttg atctggagaa ccagcatatg tggtattaca aagacggaaa
     8281 agtggccctt gaaacagata tcgtttccgg aaagccaact acacctacac ctgctggagt
     8341 cttctatgtt tggaataaag aagaagatgc aactttaaaa ggaacaaatg acgatggaac
     8401 accttacgag agtccggtaa actactggat gccgattgac tggacaggtg ttggtatcca
     8461 cgattcagac tggcaacctg aatacggtgg agacttatgg aaaacccgcg gttcacacgg
     8521 ctgcatcaac accccaccaa gtgtaatgaa ggaattattc gggatggtcg aaaaaggaac
     8581 gcctgtattg gtcttctaac aaaaataata tataaaaaga aagcacccgt acgattggta
     8641 caggtgcttt ctttttatta tctaacaatg tgctacttac atttcagcag cagtaaagac
     8701 ttctgaaaca tcatcatcgt cttctagctt atctactaga cgttccaatt gctctttttg
     8761 ttcctcattt aaatcaatcg tcgtttgtgg aatcatcgtc aattctgctt gcgccaaagt
     8821 gaactctttt tccaactcat ctcgtacagc agcaaaatct tctggtgcag tataaatctc
     8881 gaatacttca ggagaagttt gcagatcttc tgcccccgcc tctaatacat cctcaaacat
     8941 cgtctcttca tcaacagtca acccatcgcg ttcaatagcg atatatcctt tgcggtcaaa
     9001 caagtaactg actgaacccg tttctcccag tgatccaccg tttcgagtaa aagctactcg
     9061 gacatttgtc gctgtccgat tacggttatc tgtcaatgca tggactaaga ttcctactcc
     9121 acccggacca tatccttcat acgtgatttc atcgtaattc gtttcatcta cggcactcgt
     9181 agccttttta atcgcccgtt ccacattgtc gttaggcata tttgcagctt ttgctttgtc
     9241 cacaacaagc cgcaaagctg gattcataga tggatcagga ccgcctgctt tagctgccat
     9301 ataaatttca cgagaaagtt tctgaaagac tttccctcgt tttgcatctt gggcattttt
     9361 tcttccttgg atattatgcc atttactatg tcctgacatg atttttcctc ccttgttctt
     9421 ttgagaatac acttctattc tagtactatt tcacaaaaaa acaaagccac agccaatcta
     9481 taaacgcttc ttgaatgccg caaaaagcac acataatatc acagcagtca gagcaccgat
     9541 cgaatccaac atgacatctt ggaacatagg tgttcgtcca ccggtcatca tctgatggaa
     9601 ttcgtccaaa cctgcgtacc ctgttgctga aagccaagca aggatcgctg tcagccacca
     9661 aatttttagc ttggggaaaa cacctaaaaa caagctgcct cccaaaataa aataggtacc
     9721 gaaatgtgcg cccttcctta aaaagaactc taaaaatttg gcgtagccca gatggttgat
     9781 actcaccata cttccaccat aattaaaacg tatacccgct aatgtgtctt taaatggctg
     9841 attaggcagc cagctttcta atcgcgaaac ttgtgattgc tgtccgtaag tttgagaaga
     9901 actgtaaaat aagattataa cgactatcag tgcgattact aaataaatat ttccgttttt
     9961 catttgtttt tttgaagaat tcataagtca cctctgtatt ttatcttaga ctttttttga
    10021 agaaacaaca atcattttca tagagacttc ttccataaaa aagtgctata cttaattttg
    10081 tattacaata tgaagggagt tttttcatga gttattcatt aaattggaat ctagattcta
    10141 ttttttccgg aggcagtcat tctgatgctt tgaaccaacg gatgaagcaa ttggaagacc
    10201 aaatgaacga gtattatcac cgcgtaacaa aatggtcacc tagttctgac aatgcagagc
    10261 agttaaacgc tattcttcaa ttgcaagaaa cgattacgaa tggttttacc caatgcagca
    10321 gctatattac tgcattgctt tctgccaatg tcaatgattc agatgcaaag gtcttatcag
    10381 gcaagcttta cgcaatgctg cctcgtctgc aatcagccga aactgtattg tcaaagaaat
    10441 ttgcagaaat atctgacaat gattggaatc agatgctttc tcaatcttcg tttgaaacaa
    10501 ttgcttttcg tttaaacgag attcgtcgtg atggctcaca attattatca gaagctgaag
    10561 aaaacattat aaatacgctt tctttagatg gtctaaatgc ttggagcagt cattatgata
    10621 cgattgtagc cagcatttcg attcccttcg aacaagatgg acaagtagtc gaattatctg
    10681 ctggacaagc cttcaataag atgatgggcg atccagatcc aaaagtcaga gaaacacttt
    10741 tcgcagcatg ggaagaagcc tggaaagaaa aggcacccct cttcacggat actttgaatc
    10801 atttagatgg ttttagactt tctgactatg aacttcatgg tgtcacagat tttctacaaa
    10861 aaccattaga gtacaatcgg ttgaagaaag aaacattgtc tgtcatgtgg gatacaatcc
    10921 agaaaaacaa acaacccctt gttaattact tgactagaaa agctaatctt ttcggtaaag
    10981 aaaaaatgga atggcaagat caagatgcac ctattatttt aggcgatttg aaagaaaaaa
    11041 cctttagttt tgacgaagca gcagcattta ttatcgaaaa ctttaacaaa ttcagtccaa
    11101 aaatggctaa atttgcccaa tctgcttttg aaaagagctg gatcgaagca gaagatcgtc
    11161 ctgggaaacg tcctggtggt tattgtactg agttaccaga aacaaaagaa tcaaggatct
    11221 ttatgaccta cagtaattct gttaatgagg ttgcgacact tgctcatgaa ctaggacatg
    11281 ctttccatag cagcacgatg tgggatttac cttctttaaa tcgcgaatat gccatgaatg
    11341 tcgcagaaac agcaagtaca ttcgcagaac tgatcgtagc cgatgctaca ttaaaagtcg
    11401 caaaaacaaa agaagaaaaa atcaatttgc tagatacaaa actgcaaaat gctttagcta
    11461 tgttcatgaa tatccattcc cgattcattt ttgaaaatcg attctatgaa gcacggcaag
    11521 aaggattagt ctcagaagat aagatcacag agatgatggt tgaggcgcaa aaagaaggct
    11581 atcaaggagc tttagctact taccatccat atttctgggc tgcaaaattg catttcttta
    11641 ttgatgacgt accgttttat aacttcccat acacatttgg ctacctcttt agtttgggca
    11701 tctatgctta cgccaaccaa aagggagcaa attttgaaga agaatatatc catttactac
    11761 gcgatactgc ctcaatgacg acggaagatc ttgcgaagaa acatttgaat gtcgatctta
    11821 cccaaccaga tttttggcaa gcaggtatcg acatggtgac tgaagatatc caaacgttca
    11881 tggaattgac agaagaattt atctgatttc tattttatca caataaaaaa agagttagtc
    11941 cagaatgggc taactctttt tgattaatca ttcttacatg ctgacagcaa agtcaggggc
    12001 aaaccattga actgaaccaa cttttacgct ttcgcctggt tgaggagcat ggatatattg
    12061 tcctccgcct aaagcaatcg ctacgtggta agttccgcct tgtgaccccc agaataacaa
    12121 gtctccagct tttgcttgag aaactgaaat ttttgttcca gctgattctt gaggaactgt
    12181 ccaaccaccg atgtcacgac cagttacttg catgtaaaca tagcgtgtga accctgagca
    12241 gtcaaagcca cttggatctt taccgcccca aacatatgga gtaccaatat atttgtaagc
    12301 ttctgctacg attgctgcac cattcacact tccagaagga gctggagcag gtgttacagg
    12361 ttttggttgt tcaggtgttg gtgttggcgt tggagccgga gtactacttc ctgtgccgtt
    12421 tcctgtatca acactttgat ctgttgatgg tgttgtaggc gcaggtgttg ttgattcttc
    12481 tgttgttgaa gattcaggca ctgttgttga ttcttctgtt gctgaagatt caggcactgt
    12541 tgttgattct tctgttgctg aagattcagg cgctgttgtt gattcttctg ttgctgaaga
    12601 ttcaggcgct gttgattctt ctgttgaaga ttcaggtgtt gttgattctt ctgttgctga
    12661 agattgtgta gctgatgatg attcttcagt cattgaagat tgtgtagctg atgattcttc
    12721 tgttgctgag ctttgtgcag tagatgaagc ttgagtagct tgtgcttcag cctctgcttg
    12781 ttcgcgtgct tctttttcag ctttttcttg tgcagcttgt tgacgagctt gttctgctag
    12841 acgttgttgt tcacgaatac gagcttgctc tgcttcagct tctgcttttt gacggttcaa
    12901 gtcagctttc ttgtcttcag cagttgcttg ttcagcagct aatgaagttt tcagtacgtt
    12961 caaatcagct tgtttagata aaagatcgcc tttttgagat tccaaagcag cttggttttc
    13021 agctaattct tttagtttag catcgttttc agcttttttg tcttctacag cttttttatc
    13081 ttgtttttgt tgttccatca agtcgttgtt tgcttttacc atcgttgtca ttgcttgaac
    13141 gcgtccaata gcatctgcta atgaatcagc attcaataca gcatcgatgt agtttgagct
    13201 tgtgttgctt acttgtgctt cgcgtgcttg tttttggatt gtatcttcac gtttttcaat
    13261 acgttcttgt aaatctgcaa tgtcttttac taattgtgca gattcttgac gcaatgtatc
    13321 ttgttttgct aataggtctt gtgcttgtgt gttgatttca gaaacttggc tttctaaggc
    13381 atcgatttgt gattgtgcat ctgcttgttg atttttcaag tctgcaatct tttgatcttg
    13441 ctgttgtatc tgagaatcaa aatcgtccgc cgcggcagcg atcggtgatg ctacggcagt
    13501 caaggtcatt gaacatacca ttactgctga tattaaactc tttttcactc ttcattcctc
    13561 cgactggctt aatttaataa atatctaaat atatttaagt ctttcttaac gatataaaaa
    13621 ttgtaccata gcagtgtgtc aacgatataa tagttatgtt acaaaaatgt ttcaaaacaa
    13681 cttttaaaaa tgacaaaact gttcgtttca gaaaatgatc atgaaaaagc cgcgttaagt
    13741 ctagcatttc ttaagataac aacgattctt tttcgctttc gacctaaaaa taaaaaaaga
    13801 aattattcat cactgaataa tttcttaaag ggaaaaatga ataatacaaa tataatcatg
    13861 ttcaacagca gtgtaggtcc taaaaacctt gtaataaaat acgtgttatt tactactgct
    13921 aatttaaaaa tcaactggac caccatcgta aacaattcat aacccgtgac aaagatgatc
    13981 atactgaaaa attcagtaaa gatgttgaca tggataacgt ctttcattac atacatcaac
    14041 catacaatta aagggagagc tactgcatag ataccgataa cgccaatata gtaagcgtca
    14101 tagatagccc cgagaacaat cgtggtaatc agtaaataac gtttttcaaa cgccatactg
    14161 caacataata atgccaatat cagaaagtgg gcatttgaca tataggtgcc tttcgaccac
    14221 tcccctagca ttcttgttaa atgaccatct atcaacatca aaaagaacaa aacgattggc
    14281 agatagtact tcattgtttc tttcttcaac atggactaat cctccgctag ccgttggaca
    14341 atcgtcacaa ctgacacgtc atacatttct gcataaggtt tcacataaac ttcccgatcc
    14401 agcccataac tatctggttt cgttttaata accgtaccga tcggcaagtt agctggagaa
    14461 ttccctccaa gaccggatgt ctggacaaca tccccttctt tgatatccgt atcaccagtt
    14521 aattgggtca ccactaaggc atggagcttt tcatcatagt ttttaagcaa accaaacgct
    14581 tcgccattag ccgaagatac ccgtactgga aaatgattgg agctttcatt agaggatgac
    14641 agtaattcga ttttagacga agccgtattg acctcgatca ctcggccaat caatcctttt
    14701 tgcgacatga ctgccatatt tgcttcgata ccgtctttag aacccttgtc tacgatcagc
    14761 atgtcctgcc aactgtcagg cgaacgagta atgacatttg ccgttacttt ttcgtaacta
    14821 gtcagcgtct gattcaactc aagttctttt ttcaacgctt cgatttctct ctcgtaattc
    14881 ttgttttgct gtgaaacgtc atcatagcta tcgatttttt cttttagccg ctcattttcg
    14941 gcataagttg aaaaaagatt atggacagaa tctactccat ttcgtaccca ccgtactggg
    15001 gcagagattg ttttatcaac cattgcaaca ctgtcattaa cgatggattg gaagaaattg
    15061 ggctttcctt cattcgctcg ctgcgcagcg gtcacactta gtatcgtcac cacgatgata
    15121 acaataacca aggtaataat aatattttta ttaggattga acttcttcac aaagacacct
    15181 cgaattaatt agactcttgt gttcaatttt atcactaaat gccgtgaaac aaaaggtaag
    15241 ttgctcctct taagcttttt tttagcaaat aatagattct gtcattttta gcctgaaaga
    15301 caaatttttt tatttgttta tggatcaatc tttgctttga ataactgcaa tcataccatc
    15361 tgaaaaagaa aattctgcgg tatctgaaag aaattcgtta cttgcataag atgtcggata
    15421 ttctacttct ttattttcca atacatactt gctgcctttt aatgtaagat tcgagacggg
    15481 agtcaagcag caataagcta aatacttcat atctggtatc tgtcttacta tatgctctcc
    15541 aggcaaataa taagacaaat aattttgaca atctctgata ataagctgat gggcaaaggg
    15601 tttgaagcga ggttccatac ctaaccagag attggctaaa agatgatcca atcttccgcc
    15661 cgtagctcca atcaaagtga cttccgcttg cgggtaacgt tcaaatgttt ttaagatacc
    15721 taactgtgta tctgtatcgt ctttttctgc aggggaacgg gtatactctt tggcttttgc
    15781 aaatacttcc ttctgctctt cgttcgacaa tgaatcaaaa tcacccactg ccagatctac
    15841 tgggtagcct gcttgaagca ggtacagcgc accacggtct atcccgacat aggcatcgta
    15901 tttggacagc agagactgcg gccagttttg cggattacct ccagcagcta ataaaatttt
    15961 catttagtct aatgcctctt ttaatcgttc gatttgtccg actggatttt cggctccata
    16021 gatatatgat cctgcaacga agacatccgc ccctgcatct ttacagattt ttgctgtttc
    16081 tggaacgatt ccgccatcta cttcgattgc ataagtccaa ttgttctctc tacgtagatc
    16141 agcgagttcc gtaatttttt ctactgtttc ttcaataaag ctttgtccgc cgaacccagg
    16201 attgacagtc atgactagta cttgatcagc caaaggcaaa acatgtttga tcatggagac
    16261 tggtgttcct ggattgataa ctactccagc tttaactcct aagctttttg tcatttgaag
    16321 tgcccgatga atatgaggag ttgcttcgac gtggacagta atgatatctg ctccagcttt
    16381 tgcaaatgct ggaatgtagt tttctggatc aacgatcatc agatggacgt ctaacggtaa
    16441 tttagtaaca ggacggattg cctgaacaat atttggccct aatgtgatat ttgggacaaa
    16501 tcggccatcc attacatcta cgtggatgta atctgcgcct cctttttcca ctaactcaat
    16561 gtctctttgt aaattggcaa aatctgcact caaaatggat ggtgctatct tcattcttgt
    16621 ttcctcctat tttcgtttac cttctttttt tgcatacatt ggttttcttt tttcgatttc
    16681 ttgtaagaat tgcaaataat tgtcgtaacg tgtctgtgcg ataccacctg tttctactct
    16741 atgttttact tcgcagccag gttctttagc atgcatgcac tctctaaatt tgcaatggac
    16801 agaagcttct acaaattcag gaaattgtct cggcagttct cctgcttcaa ttgtcaaaaa
    16861 gtcaatcgag ctaaatcctg gtgtatctgc tactaacccg ccatagatag gaatcagttc
    16921 cacatgacgt gttgtatgcc gtcctcttcc aagtgcatca gaaatctctc ctgttgctaa
    16981 attcagttcc ggtgatattt gattcagtag cgtcgatttt cctgctccag actgacccat
    17041 aaagaccgat agccgttctg ggaagtaacg gatcagttct gtcgtatcac ccgtatgatt
    17101 gggtacaatc accggatagc ctattttagt ataggtttgc ttgatctctt cgattttttc
    17161 tgggtcgtct gacaagtcgg tctttgtcaa aaaaataatc ggttcgatcg actcgtattc
    17221 caaggtaact agaaagcggt caagtagatt ataagaaaat gcaggctcta cgatacttgt
    17281 gacaatgatt ccaagatcaa catttgtgac tggtgggcga actaattgat tttttctcgg
    17341 caagatttcc agaagatagc cgtctgtttg attttcactc tcaaagatca cctgatctcc
    17401 gacaagcggc gtgattttcc gattccggaa atttcctctt cctcttgttt gataggtctc
    17461 tccgtttgcg tatacatagt aaaaaccgct gattgctttt ctgatttgtc ctttgataag
    17521 tgccacctcc gtttttgttt attttaccat acgaagagga aagtaggtgt tattccatgc
    17581 aaacttctca aagtatatgg cttgaaaatt gatcaaaaga gatagaaaca caaaaaagag
    17641 aatgcaacaa atcaatgtcg cattcccttc tgatatattt tattcagcac ttattttgaa
    17701 ctttctgagg tgctagctga tgaacttgtg gagctggaag attcttcttt tgaagagttg
    17761 cttgtgctac tggagctact tgagctgcta gaactgtctg aagagtcttt ttcagaacta
    17821 gaagacgctg gatctggtcc tttagagtaa attacgttca caaccattcc tttggtaatt
    17881 gtcgttcctg cccctggtac tgtacgaatc actttatctt tatccaccgt gtttgaatat
    17941 tcggaagtct catgcccgat atattctgca ccgacactag ctaaataact ttgtgcttcg
    18001 ctttttgtat agccggagat atcattcaat ttaggctctg ttccttggct tacgacaaaa
    18061 ttgatgtaag tttcttctgc tacaacttca cccgaggctg gatcctgact gatgatcgaa
    18121 cctgatggta cagtgtcgct atagtcataa gtagcactaa tattattgat attgaatcct
    18181 tgtgtaacaa gccaattacg aacagtttcg tagttttctc ctacatgact actcaaagtc
    18241 actgtttctg gtcctttgct gactgtgaaa gtaatcgtgt ctgttttagg atcgacttct
    18301 gtatcttctg atggagattg cgaaatgatc attccgcttt ctacctctga actgaattcc
    18361 tcttcttttt gaatcaagtc agcagaaaac cctaattctt tcaaacttgc ttcggcatct
    18421 tcataacttt taccactgta atcatctaat ttgatttttt tcgtgccgct actaatatac
    18481 agagtgactt cgcgtttttg cttgactgtc gttcccgctg ccgggtctgt cttcactaca
    18541 ttaccttctt cgattttgtc atctgcaatt tcttctgttt ttgttgctac ttctaatttc
    18601 gcttttgcga gtgtttctct agcagaagct tcagaaagat cagaaacatc aggaacaaca
    18661 acttctcctc gcccgccaga agcaaagaat acagctgttc cgattcctaa aacagcaaga
    18721 atagctaata ggatcagcca ccattttttc tgtcctttct ttttaggatc aggcagaact
    18781 tcttcctcct ctggtaaggt ttcgttttct ggcttgtctt ttggcagagg catagatttg
    18841 aatgaatcag gcattggtgc atcttccgta aggggtgtga taacttttgt ttcattatcc
    18901 atcacatgag gctgccattt ttcttcgttt gcacgagctg gtgataatac tgtagaaaga
    18961 tcatcagaca tttcctcggc tgacttataa cggtctgcag gttctttagc cgttgcccgt
    19021 aaaatcacgt tttccagtgc ttgtggcaca tttggatcaa gtgcttttat agatggaaga
    19081 tcatcttgaa aatgttttaa agcaattgtt acagcagatt caccatcaaa cggtacgcta
    19141 cccgtcaaca tctcataaag gatgatccct aaagcataaa tatctgactg cttcgttgcc
    19201 atacttcctc tggcctgctc aggggacaga taatgaacag atcctagcat cgtattcgtc
    19261 tgcgtgatcg atgtttcaga taaagcgata gcaatcccga aatccgtgat ttttacaaca
    19321 ccttcattat cgatcagtac gttttggggt ttcaaatctc ggtggatgat ttgatggtta
    19381 tgagctaaag aaatagcaga aaggatctgc tgcataatat ttactgctgt ttcataagga
    19441 acaggataat gtgtttggat atatcgtttc aagtccattc ctttgacgta ttccatcacc
    19501 agatactgca tgttgtcttc ttcaccaaca tcgtaaacac taacaatgtt tggatgaacg
    19561 agttctgttg ctgctaaagc ttctctctga aatcgtcgaa tcgcagtctg gtcattttga
    19621 aagtcaaatc gtaagacttt aactgctaca tcacgatcca agattaaatc gtgcgccaaa
    19681 aatacatttg ccataccacc gctaccgata tttccagtga tttgataacg gccatttaat
    19741 tttttgccca actcgatcat acactgtcct ccttatattc gataagaaga acggtgatat
    19801 tatccgctcc gcctgcttca ttggcacgat caatcagctc agttacttta tgagacaatg
    19861 ggccttcttg attgatgatc gttcctattt cttcttcaga cagcatgttc gtcaagccat
    19921 ctgaacataa cagcagtcga tcttttagtt gccaaagatg agtagaaaca tctacttcga
    19981 ctgttccagg catgccgaca gaacgtgtta aaatattttt ccttggatgg ttcactgcca
    20041 tttcttcgct gatttctcct gatttgacta gttcattgac taaagaatga tcctccgtca
    20101 attgcatgat tttatcttca cgaatcaaat aagctctgct atccccaaca ttggcaatcg
    20161 tgaaccagtc atctgacaga gcggccgcta cgattgttgt tcccataccg ttatattccg
    20221 gctgttcttg tccacgctga taaatcagca tattttcttc ttgaatcgat tgaatgaacc
    20281 attgggcgat tttttcatcg tcaacagctt cttgtttttc ccaagcttcg cctagattcg
    20341 ttaccgccat ctgactggca atgtctccgg cacgatgacc acccatcccg tctgctaata
    20401 cagcaagctt gagacctgaa cggctggtaa atacactggc atagtcttga tttgtgttac
    20461 gtcgtcttcc tacatctgat tgatattcaa tctgcatttt ttcacctcat tactttttgc
    20521 gcaaacaaca aatgaaaaat ccatctgtgt aaaactgatg cggatacaaa gtcaacattt
    20581 tctcttcgat tgatgattgt agcataggat tggtcaaaac atcaatcctc tcaaaatcag
    20641 ggtgtctttc caagaaagca gaaacaacgt cttggttttc ttcttttaag atcgtacaag
    20701 tgctataagt cagaataccg gaagacttta aagttggtgc gcagctttct aaaatttcca
    20761 attggatttt tggtaaattc gctaattcat ccggctgttt tttataacga atatctggtt
    20821 ttcgccgaag cagcccaaga cctgaacaag gcgcgtctac taaaatccga tcaaactgct
    20881 ctgcttcgaa ctgtttactg acttctctag catccatctt ttgagcgtag acagcagaag
    20941 agacacctag acgtgctgca ttatcttcaa tgagtttgac tttgtgttcg tgtacatcta
    21001 aagcgaccac ttctccaccg catgacggat ctaaaaaaga agcaatatgc gtcgtcttac
    21061 ctccgggtgc tgcacaggca tctaagacat gatgttcagg ctcgatttgc atagacggcg
    21121 ctacaagcat cgaactttca tcttggatcg tcatgacgcc atcgtggaac aatgagctag
    21181 aagctaaatg cccttttttc gctactatac cgtatggact gacttggctt ttttcagctg
    21241 tgatctcttc ttcttgcaaa acagtgatgg cctgatctct ggaaagtcgg cgtgtatcta
    21301 ctcgggcact agcatggcta ggctgatata gcgataaacc gagcttttca gtttcttcgt
    21361 atccgatttg atccaccagt cgtttcgtca tccatagagg caggctgatt ttgacagaaa
    21421 gtctttcgat aggatcagca atgccttgca gatctggtgc tccttgacgc tgataattcc
    21481 tcaagacacc atttacaaat ttccctgtcc caggatttcc ttttactttt gcgatttcga
    21541 ccgcctcgtt taaaatagca tgtgcaggga ctttatctaa aaattctaat tggtatacag
    21601 aaagataaag aagcatcttg acccagtcat ccactttttt tgctttagcg atcaatggtg
    21661 caacgtaaaa ttccaatagt cgctgatggc taatcgtacc atagatcaat tctgtaaata
    21721 aacccacatc tttttgattt agctcgcttt gtttgatcat ctcattcaac aaaagattcg
    21781 agtaggcgcc acccttgttc actcgctcta atgtcagtaa tgccatatat cgtactgatt
    21841 ttttcatttt tttagggatt tttttcatta tttcacctgc tgaccaatct gaacttgctg
    21901 accgcttccg ttaaggaaat catgtacggc ctgttttcct ttaccagctg gctgtaattc
    21961 attgatggct aagacggtct gtttaccgca agcaatccat agattttttt tgtctttccg
    22021 gatgatcgta ccaggtgctt tgtctgtagt ttctgccaat acttcaaccg ataataattt
    22081 ccagcgtatg ccctcatagg tcgtgaatgc aattggccaa ggacgcattc cgcgtacttg
    22141 gttgtcaatt tcttctgcag ttttatgcca atcgattgct tcttgttccc gtgtgatgtt
    22201 tggcgaaaaa gttgcttctg actcgtcttg aggtactggt ttttgtccat ccaaaatatt
    22261 gggcaatgtt tctagcaata gctttcttcc taaaaggcta agcttttcaa acatcgtccc
    22321 tacatcatct tgtttagtga ttggtatgct ttcttgcgca tagattccgc ctgcatccat
    22381 ttttttgatc atttccatga tcgttacacc agtttctttc tccccattca tgatggcata
    22441 atgaacaggc gcacctcccc gatatttagg aagtaaagaa gcatggacat tgattgcccc
    22501 taattttggt acttgcaata acttttcagg caagaattgg ccaaatgccg cagtgacgat
    22561 cacatcaggc accaattcaa tgatttcttc catttctggc gaaccggaga ttttctctgg
    22621 ctgcagcaca cggataccgt gcttcagagc cgcttttttt accggtgtcg gcgtaattac
    22681 tcgtttccgc ccaactggac ggtcgggctg tgttaccact gcctggatct cgtaacctga
    22741 ttctatcagc ccttcaagaa tcggcacaga aaaagcaggt gtgcccataa atactagctt
    22801 agtcatcaag atgttcctcc atatattctt caagttcttc ttccgggatc tgctgtatca
    22861 ttttctcaat gaacaagata ccgtctaagt gatcgatttc atgctggaag gcacgggcta
    22921 aatagccaaa agccgtcact tctatctctt ctccgtctcg atcataataa cgcacggtca
    22981 cttcatctgc tcgcttgacc gttccataga catgcggaat actgagacaa ccttctacgt
    23041 caaggctttc accttttgct tcgatgattt ctgggttaat cagttcaaat ttctcgcctt
    23101 catctacttc gatcacagca attctcttgt tttgtcctac ttgaggagca gcgatcccta
    23161 ttccgtcatt cgcaatcatt gtctcatata aattgtctaa taaagcgatt gtctcgtccg
    23221 tgattacatc gattggctga gcagttcttt ttaatttatc attagggtgg atcaatatag
    23281 gatagcgcat aatttggccc tttcttaaat aaagtttaat ggttcgttgt caattgccac
    23341 aaaaagacct tgtcgctgtt ctttctggct tccattcaaa atttcatcca aaacatgagg
    23401 aagattcggt tcatgtttat atttgatgat tagttgataa taatatcggt ttttcactct
    23461 caaaatcatg ctgggcgttg gccctaatag caagctttga gggcttaaca cttcttttag
    23521 ctgattggca atctgaaaga ttttctttgc cgctacttgt tcttcaggat ggctggcggt
    23581 tattttcaca gtaaagtaat aaggaggata gcctccacga tgccgtacat gcatttcctg
    23641 ctgataaaac tgctcataat tttgtttttt agccaattct atggcgtaat gttgaggatt
    23701 gaatgtttgg atcacgactt cgcctgcttt ttccgctcgc cctgctcgtc ctgaaacttg
    23761 ggtaagcaat tggaatgtat gctcactaga acgaaaatcc ggtaaattca gggaggtatc
    23821 agcattcaac accccaacta atgtcacttc tggaaagtcc aaacctttag caatcatttg
    23881 tgtccctaat aatatatctg cttcttttgc accgaatttt tgtaaaatct gttcgtgtgc
    23941 cccttttctt cgagtggtat ctacatccat acgcaaaatc ctagcttgcg gataaagttc
    24001 tcggagttcc tcttctactt tttgagtacc tgttccatag tagcggattt tattgccgcc
    24061 acaattagga cagcgattgg gaataggttc ttcatgtccg caataatggc agcgcattga
    24121 acgtgtatcc atatgcagtg taagagagat atcgcaatta ggacatggaa gaacaaatcc
    24181 acagtcccta cacatcacaa aagacgaata tcctcgacga ttgagcagta ggacagtttg
    24241 ttcttttttg tctaatcgat tttgtatttt ttcctgcaaa ttagcagaaa aagtagaggt
    24301 ccgatgattc tgaaactctt cacgcatatc tacgatctca atagctggaa gctgtgcagc
    24361 tgctttggct cggtggttta actgcaataa ctgataaacg cctttttgag ctcgtgctct
    24421 tgattctaaa gagggcgtcg cgcttcctag aactattggg caatgatgat atttactgcg
    24481 ccaaattgct agatctctcg catgataacg cggtgtctca tcttgtttgt aacttgattc
    24541 atgttcttca tcgataatga tcagcccgat atttttcagc ggggcaaaaa tagcagatcg
    24601 tgcgcctact actacttgcg cttcttcccg ctcaatcttg cgccattcat catatttttc
    24661 accttgggaa agtccgctat gtaagaccgc aactgattct ccgaagcgtc ctttgaatcg
    24721 ctcgaccatt tgcggagtca atgcgatttc aggcactaac ataatagccg ttttattttc
    24781 tgacaaaaca tcagcgattg cctgaagata tacttctgtt ttcccgctgc cagtgattcc
    24841 ctccagcaaa aagacctgat ctttttgctg ctgacctgct gtgatgattt gttcgacagc
    24901 atttttttgt tctgcattca gttccaatgc cgtcgtttgg tcaaatactc ggtctttata
    24961 agggtcgcga taacgttctg attctataaa ctccagccag cccttttctt ttccttgatt
    25021 caagacagca gtactgattc ctttattttt gaaataagca attggttctt cttggctcag
    25081 ttcagataaa cacaaaagaa gttctttttg tttcttggca ttttgccgta attttgccca
    25141 ttcttcttcg atttgctttt cagttaacaa agaacggacc aaacgaatcg tttttacttt
    25201 atttcgtgta tggacttcat atttgatatc tactaattgc tcttggcgcc agcgaatcaa
    25261 ttgagaaagg ctgccttctt cttctgcttc cttccaggaa atcacttctc gctgatcaaa
    25321 caatacctga acttctggtg tgtctgccaa aggatagatc aatttatcat attctgcttt
    25381 catcacactc ggaagcatcg tttgtagaca agtgattttg aatgcgtaag taattttttt
    25441 catataatca gctaatgaaa gtaattcttc attgaccacc ggagcaaggt ctagcaaacg
    25501 aataactgcc ttcaaagatg gattagctga ttcttctgat ttttggatag ccataacaaa
    25561 tccttggaca tgacgattgc cattaccaaa agggacttct actcgcatcc cgacttgcaa
    25621 agctgtttcc atttcagagg gaaccaaata agtgaatggc tgatcggtct gcatagtagg
    25681 tacgtccaca atgacttcag ctgttaatgt catggttcgt ttcctccttt ttttgtcttt
    25741 tcttatcaag cgacaaaaag ttccttgcct attttactag agatcgatgg atttgtctta
    25801 tcttctgcca aatcctaaca aaaaattaac agcagataag aaaaaaaaga actgtgtcaa
    25861 aaagttcaga cacagccttt tctaccagac aataagtggc gaatgtttga aaaggaaaca
    25921 gctgggtttg cttttctttt tagatgttcg ctctattttt ctttattaca ttttttgctc
    25981 ttcacggata cgtgattcca attcgtgttg ttcacgctct ttttgtgctt gacgttcctc
    26041 ttcttccatc ttcaaacgtt cacgttttaa ttcaggatgt ggatcattga ctacatttcc
    26101 tgcatcgatt tcttctaaag cttgaccgac gctttttact gaatcaaaag aatctagagt
    26161 aggctgtgca cttgcatcca attcatgcgc gcgtttactt gctaaaatca ctagagaata
    26221 ttttgaattt acacgatcta atagtgagtc aattgaaggt tttaacatca taagactaca
    26281 tctcctttaa cattttaata tatttaccga ttacgcgatc tactcgaaaa tgttcactgg
    26341 caataatgtt tttgattcgt tcaactgcta gcggcacttc atcatttaca acagcatagt
    26401 cgtaaagagc catcatttcg atttcttctt ttgcaacttt cattcgtttt tcgattactt
    26461 catctgcatc tgttcctcgg cctacgatcc gtgattttaa ttccgctaaa tctggcggag
    26521 tcaaaaagat gaatacacca tcgggcactt tttctttgac ttgttttgct ccttggactt
    26581 caatctccaa gaaaacatct tttccttcat ccaatgtctt gttgacatat gacaaaggag
    26641 ttccataata attcccgaca tattctgcgt attccagcat ctctccagct tcgatcattg
    26701 attcaaactc ttctttgctt cggaagaaat aatcaacgcc ttcgacctcc ccttcacgca
    26761 ttttacgggt agtcattgag atcgaatact gaaaatcatt atcctcactc tcaaaaattg
    26821 cttttcgtac tgtgcctttt cctacaccgg atggaccaga caacacgatt aataatccac
    26881 gctctgacat gacgacgtcc tttcaactag tctttttgta cttatcgttt attttgcact
    26941 ttttttctgc aactttcaag gttatcttta attatagcta gaaattggta aaaaagctac
    27001 cctgtaccaa gaggtcgtaa aaaagctgtc ctgcatcaag taataagaac agccaaacat
    27061 aaacagcttc tcatgttttt cgtttggttg gggttattat cgcagatgca agcttttgaa
    27121 cgccgtgtat aagaggtcgt gaagaagccg attagtttcg agtatgaaga aaaattttgt
    27181 gaaaatagtt tctcacattt ttgcataaat ttggcttaat accgagaaaa tggtttttga
    27241 acatcactta ttcaaaataa aaaaagattg tgacacgaat tttgtcacaa cctttaaatg
    27301 tattcctaga gcaaagaagc taagcgaaga aatccaattc tgtttattta ttgtatttct
    27361 ttgttcagca tccttgctaa aagcccatat tggacaaagt attcactaca cccatgatct
    27421 catcttttcg agaacgtgct ttttctgtcg gatcttctac ctcttctgtg tcaattcgaa
    27481 caataaattt ccgattcatc gtcacgtaag caccgtcgtt gatcaaaata aacagttcgt
    27541 tctcattata gtctgcacag gcatctttcc atacgtcaaa gccgtttttt tcagaaggga
    27601 tatcccgttt aaccacagtt tttccactta attcgtggaa cgttacgtca taatatttca
    27661 ttatctctac ctctttcgtc attcaatatt ttgtacttgt tctctgattt tttctaagat
    27721 cgtcttagac tggacgactt gatttttaat ttcgattgca tttgatttgg aaccgatcgt
    27781 gttgatttcc cgattcattt cttggatcag gaaatctaat tcccgaccta caggacattg
    27841 tgcttcgata agttcatcta gtttttgtac atggatcgcc agacgatcca gttcctcgtg
    27901 aatatctccg cgttccaaca agatggctag ctccgtcaac aaccgttgct catcgacttt
    27961 cgctccaaga aattcctcca gctttttttg ataccttatt tgaaattcct tttcatagat
    28021 ttctacaaaa ctatcgagtt tttttagtac ttctttgaat tcttcagtat tctttttcaa
    28081 cactaaagct aaagcttgac cttctttttc tcgactcgcc tcaatagaat caagtgcgct
    28141 tttcaatgta tctagagcta actgtgccaa ttcttccatg ctctcttccg gtttttcttc
    28201 taccgttacg aagttttcgt ccagcacgaa gcgttccgcc atccgactaa ttgacacttg
    28261 attttgacta ccgtatcgtt gttgaattcc gtctgtcaat tgagtgataa gtgaatcaat
    28321 caattcccag ttgatgacga tctgtttttc gtttttgcca atataagcta gattcacaaa
    28381 tacttcgatt cgaccgcggc ttaactgctg tttaataagc tgacgaaaat cgttttcaac
    28441 aaagttcagg attttaggac ttcgtatttg aacatctaga aaacgctggt tgacactttt
    28501 tatttcaata tctaactgat acgcttttgt ttcgcggatg gctttgccaa aaccagtcat
    28561 acttttcatt cgacagggtc cttcctcatt acatattttc tattgctttt tgcagttgtt
    28621 caaactcttc gttcgtcaat gtcaggcctt tccccatttt ttcatggtct ggcgcccaat
    28681 ctcgaagatc gaattttggc gggcgaccat tccagctgac taggttcagt tctttacgcc
    28741 accccttgtt gttttctgac aatatagcga tttcttctac gatttcatat gaaaattctt
    28801 gtgccatggt tgttcctcct tgagataata taaatatagg atcagtaatg tgtcaactgg
    28861 ttgcttaagc aattcatgct gcaaacgatc cttgcgtttt ccttgtagca gataatcaaa
    28921 ataaacagtt ttcgtgcgct cattttgtat tttttgctgg tattttcgat accttcgcca
    28981 ttcttcagta cttaattgac caatttgtgc caataaatca tccggcatac tttttatatc
    29041 ttgttggcag atcatttgaa tcatcatgtc tacttgatag atttcattgg gcagtaaaaa
    29101 atgttttttt gctaaaagtt ctagcatcgc caatccgcat gtctctggta cgaccctgtt
    29161 cttatataaa ttacgcagtt tttgccaaaa ttttatgctt ttgaaaaaag ataattctaa
    29221 ctgaatttct tttgttaagt aagacaaaaa tcctcgccag tatgttcttg caggtttaac
    29281 agaatgaaat gtataccagt tcccatagta atagcctaat cgttttttca tttccagata
    29341 gcctaattgt aaatttaatt ggttcctttg gctatcaaaa agcagaaagc tgcctagtgt
    29401 ccacaatgtt tgacatttcc aatggataaa aatgtctggc atacgaattt ttttcgctgg
    29461 gcctggccct tgcacatcta cgacaaatgc ctctgttgct cctttgttaa tcgcaatatc
    29521 aataggtatt ttgttgcgat acccaccgtc tgcatattta ttttttccga tctttcgata
    29581 agccatagcg ggataaaatg aagcagaggc gagtatccaa tctggtattt cttctgtttt
    29641 tgtctgttgg atatcagtca ctacttccgc tagttctggc agtttagtag acaccgtata
    29701 taaacgtatt ttgggattat gtttgagtaa ggacaaatcc aaattgtctt tgaccatctt
    29761 ttccaatgga gcagaagaaa ccccgccttc aattagtgct gttttcgtca tttggcgtct
    29821 ttctcgatag aagcgttcac gaaggtcttc aactgcagcc atttctggta acgcaagcac
    29881 ttgtcttgtc gacagctttt tccataaatt cacagctttt ttcatatctc cttgtaaaat
    29941 caatgcgccg ttcaacgcac ctaccgaggt acctgtgata atctcaaagt tgatatcatg
    30001 ttccttcagc gcctgccaca caccaatctg ataagcaccg tgcgctcctc ctccacctaa
    30061 aacaagtgct gtatgataga ccagcttttt atttaattgt gtggttcctt gatagccgtt
    30121 tttatgaaaa atacgcaacc attcttctga tagaggagat tgaaataaaa aacaacagtt
    30181 ttctttaata aaaaaacgag cgcaagcttc catataccga aaaaattttt tccattcaaa
    30241 agtttcacct tcaatcaaca gattattaaa aataacatct cctctacttt tagcaatagt
    30301 aaaatagacg gtatattttt tcgatactgc tttaaaagca cattgctttt ttgcagaacg
    30361 ccaaaaagcg tctctacttt cttgatgact ttgaaaaaat ggaaggggtt gttttgcttg
    30421 ctggataggc gaatacagcg acattttctg tgaaaagtaa acttttttta tctccatcgc
    30481 gctctccttt ttcttcaagt ataccagtac gtcataaaaa ggtcatgagt ctcgtattcg
    30541 actttgtaga ttccataaga aaacaattca taaaacctcc tttaaataaa aaaatacaaa
    30601 aatcacaaaa attaattgaa aatgagagtg ttttcaatga aaacgtgatt ttgtaatgga
    30661 attttaaaat aaaagaaaca agctttttta atgcaaatgt tgtaaaataa aagagattaa
    30721 gaggaaataa gaggaggaac atgatgactg agtcagttta tgtacatatt gataccacaa
    30781 gtaatgctgt tttaacaaaa gggctgactg ctactgactt tgcaaacagt atcgtgcatt
    30841 ttcctcaaaa cttgctcttg cttgatccat ctgcttctgc tggagaatat gaatcccata
    30901 cagggctgaa agtaatccgt ggtacagaaa atattcagcg tttcttttcc tcttctcgta
    30961 acaggcgtgg attcgatgaa ttgaagtgga tcgactttac tgatttaacg atgctgaaag
    31021 aattgacacc tttagaaatt tcagaattat tatattttgg tcatatgaaa acacatcttc
    31081 attcgccgtt tttttataaa ctacagaata attttgtata cttcgatctt aatgatcagc
    31141 tgaatcggat ttattatcgc tatttagatg aattttatag gatttttgct agtaaattaa
    31201 gttcgatctt gtcagataag atcaatgtaa aaaaatcttt tttcaaaaaa gaaacacctg
    31261 ttgaaaaaat cagtgtggaa ttattgaaag aaatgcgtaa tttgatgcag gaagggatca
    31321 ttttttcttt tgatcaagtt ggtttgatcg atggtaaata tcgtatccca atctatgtga
    31381 ttgaagatta tatttggaaa ttaaaaggca cccggtatcg agaagaggaa atcatcgcta
    31441 ctttaatcta ctctgtcacc gatcgacact ggcaattggt agaagatgcc gatctttctt
    31501 atctgccatt gaagtagatc tatttcttat caaaaaataa aaaggaaaca agaaactagg
    31561 acatgattct gtctcaagtt tcttgcttct tttttattta cagctcctgt cgtttatctg
    31621 gcacaaaaat cgaaggagtc gtttgttgga tccctaataa aacgatgatc gttacaatag
    31681 ctgtactcaa cccgctggca ccattcatta cgaaagaaaa gagccaagga ttcatccccc
    31741 atagtgcata cgctccccag aaaaccactc ctgcaataaa atgccaaaaa tatctggaaa
    31801 gtgcgccaat caatgcacca aacacgatgc tttttctaat ttgtttcgta ttatcgtcta
    31861 catagccttt ttggacattt gaagccatga gccctgctgc gcctgcaaaa gtataggcaa
    31921 tcgggtactc aatcaacact tgaacgacac ttaaataata aacttgtccc aacggaaaat
    31981 gtaggatgcc ccaaatgaaa cctgccagca tccctgcctt tgtccctctt cttaacgcaa
    32041 aaagagtcaa tggaatctgc cctaatgaaa tagaaaaact gctgccgatc tggatcggaa
    32101 tcaacgacaa caccatcgct aacgctgcaa caatcgttcc ttctacccaa actctatttt
    32161 ttcccataaa aaaacttcct cctttgaata aaaaagtcac aaaggaggaa gcagctttcg
    32221 aaaatagtca aacagctgtt tattcacaat tcctacgctc gcataaccga tacaggtacg
    32281 aagggtttag aaatagattc aatctcagcc caattggact cccctttgtg agtttattta
    32341 ttatttattt tttgtttcaa acaatggact aactggtttg ttttcatgga tacgtttgat
    32401 tgcatctccc attagtccgc caacacttac ttcatcgatt ttgtcgattt tacgatcttc
    32461 tggaagatag atcgagtctg taacaactag acgctcgata gctgaatctt ctattctttg
    32521 taaagcaggg ccggataata ctggatgtgt acatgaagca tacacactag ttgcaccagc
    32581 ttctttcaaa gcattggcag ccaatgtgat cgtacctgct gtatcgatca tatcatcgat
    32641 cagtacacaa gttttgcctt tgacttgtcc aatgatattc attacttctg caacatttgc
    32701 ttttggacgg cgtttgtcta tgatcgcaat cggagatttc aaaaattccg ctaatttacg
    32761 tgcccgtgtg acaccaccat ggtctggtga aacaacgaca acatcatcgc ctttgattcc
    32821 tttttctaag aaataattgg caataagtgg cgcacccatc aaatgatcga ctggaatatc
    32881 aaagaaacct tggatttgga ctgcatgaag gtccaaagtc agcattcttg ttgcaccagc
    32941 tttttgaatc atgtttgcta ctaatttcgc tgtgattggt tcccgtgcac gtgctttacg
    33001 gtcttgacgc gcatagccat aataaggcat gactacgttg attgtttttg cacttgctcg
    33061 tttcagtgcg tcaatcatga tcagcaactc catcaagtta tcattgactg ggctgctcgt
    33121 tgattgaata acatagacat gcgatccacg aatactttct tcaatgttta cttgaatttc
    33181 tccatcacta aattgagtaa cagatgattt tcctaactcg acgccgactg ctgctgcaat
    33241 tttctctgct aatgggcgat tggaatttaa cgcaaaaatt tttaatcttg ggtcaaagta
    33301 atgttttgac atggggacct ccactttctt tttcacaact ctagaattct acctaataaa
    33361 tagtagtcat attttttaaa taaatcaagt gattttgcaa aagtcttagt ttagataagg
    33421 gagttttttt gcatatcctt ctttattgac ttgtcttgct ctcgcaattg ccagcgcata
    33481 ttctggaata ttgtctgtaa ttgttgaccc ggcagcaatc gaactgtttt tagctacctc
    33541 gacaggtccg atgatattcg tgctagaacc aataaagctg tggtcgccga cagtcgtatg
    33601 atgtttgttc tttccatcat agttgacgaa gacaacacca catccgacat tgatttcttc
    33661 tcccagcgta gcatcaccaa cataagtcaa gtgaccgact ttagttcctt tgccgatttg
    33721 agcatttttc acttccacaa agtttccgat atggacgttt tcgccaattt cagcttttgg
    33781 tcgtaaatgt gcataaggtc ccacatctgc atgtttcttg acatggctgc tttcaatgac
    33841 tgagttttca atgacgacgt gatcctcaat cctactgtct acgatttttg aatgtgaccc
    33901 gatgacacaa tcagaaccaa tgactgtatt tccttggatt tgtacacctg cttcgatcaa
    33961 tgtgtctgga ccgatttcta cgcctgcatc gatgtatgtc gttgctgaat cgacaaagct
    34021 tacgccattg accatatgca tctggttaat tcgctttctc atgatctcat ttgctttagc
    34081 taacgcgatc cgatcattga ctcccattga ttcttcaaaa tcatccgttt gataagcagc
    34141 aactgtatgg ccttcttctt ttaagatttc gatgatatct gttagatagt attcaccttg
    34201 agcattgttt gttccaactt tgcttaacgc atcgaacaaa gcttcgttgt caaaacagta
    34261 agttcctgta ttgatttctt gtacaagagc ttcttctggt gttgcatctt tttgttccac
    34321 gattttttct acgatcccaa tatggtcacg gatgattcgt ccatatcctg ttgggttttc
    34381 tgcttgtgcc gtcaagatcg ttgcacttgc attttttcct tggtgatatt caaaaagatt
    34441 gttcagcgtt tcagtagtca ataatggtgt atcgccactg ataactaacg ttgttccttt
    34501 ttttccttcc aagaaagaag cagcttgcaa aacagcatgt cctgttccca attgttctgc
    34561 ttgtaacgca tatttactgc gttctcctag ttgtgcttta acttgttctg ctccgtgtcc
    34621 aacgatcgta atcacttcat ctggtttcgt ttcactgacc cgattaatga tatgttctac
    34681 cataggttta ccagaaacgg gatgtaaaac tttatataat tttgacttca tgcgggtacc
    34741 tttccctgcc gccaaaataa ttgcataacg tgcgtccaac tgcgtcactc ctaaatatag
    34801 tttgctgatt tcagtttatc ccagtatacc cattttttca acaaaacaag tgattttctt
    34861 ttatcccagc gttttctgga taaaaaaata gatagaccat aacgggccta tctatttggt
    34921 aaccggtgct ctcctgccaa ctcctcggtc acaagtcaca ctactcataa aacatgaaaa
    34981 gctgtttttt gaaatcgttt cttgtgactt agagttaaac ggtaggctta caacctcttc
    35041 attaaacgtt tttcaatcag ctgaccttcg gtattagctc aataaacggc aaaatatgag
    35101 gagcaatttt tccgtttatt gagctaatac tcaggacagg acgctgattt cacaacctct
    35161 agataaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg aaaaaatgtg
    35221 gaaagccatt ttcacaaaat ttttcttact actcgaaact aagcggcttt ttcacaacct
    35281 cttattctga aaaataattt cctggcacaa ccgtaatatt tttcgtttga gtatctacat
    35341 ctttgacata aagcagtgac gtataatcat cgatggcgcg atgaccgttg aattttgatt
    35401 cggcaaatac agtgatgcca acaagttctg attcgaattc ttcaatcaag ctcttcattc
    35461 cgttgactgt tccgccgcct ttcatgaaat catccactac aaggacacgt gaaccacgtt
    35521 tcaaacttct cttagacagt tccatctttt caattctttc agaagatcca gacacatagt
    35581 tgacacttac tgttgatcct tctgttattt ttgagtcccg gcgtacaata acgaatggcg
    35641 cattcagata ataggacaca gcttgggcaa taggtacgcc ttttgtggct acagtcatta
    35701 cagcatcgat tttttcacca aggtatttag aagcaatgat tcttccaact tgacgcagca
    35761 gttcaggttc tcctaacaaa tcagagagat aaacatatcc tcctggaagc agacggtctt
    35821 gttctgatag acgttctgct agagattcaa tatatttttt cgcctcttca tatgaaattt
    35881 cagggatgaa caatacacca cctgcagcac ccgggattgt ttccaaggtt ccataccctc
    35941 gttctttaaa tgttttcttg atgattccca aatcttcact gatagaagat ttagcagatt
    36001 cataacgttc ggcaaagctt gttaaggaaa tcaattcatg cgggtgattc aataaatact
    36061 gtgtcatgtc aactagacgt tcactgcgac gtactttcac ctattccaac tccttttaag
    36121 aaattttatc gttttacatt atataagttt ttcttcaaaa attcgagcat tttatgaaaa
    36181 aataagaaaa tagttcggta tttattaaaa taaaaagaag cctataaaaa atttttcgtt
    36241 tttataaggc ttcttttctt tataatttag ttattttttc ttctgaaggc aaccaatatt
    36301 cgtttgatga tatttacaat caggaacaaa gcaataaaga ctaatgtgat tgttgctcct
    36361 ggaggtgtat ctagataaaa cgagctagtt agtcctgaca gcatccctag aaatcctaca
    36421 caaacactga tagcaatcac gacattgaag cttttgccta atcgcatacc gattgctgcg
    36481 ggaagaacca tgattgcgga tatcaaaagt gcaccggcaa tcgggatcat cacagcaata
    36541 gccacaccag taatcacatt gaacatcatc gacatcaaat gaacgggtag cccatccacg
    36601 tgggcggtat cttcatcaaa agtcagaaca tacatcggcc gtttgaataa aaagaacaat
    36661 ataccgatgg caacgaataa gattcctaaa aagatgactt gctcccatgt aatggtcacg
    36721 atcgaaccaa acagatagga ttggatactt gttgctgaat tccctccgct caaattcatc
    36781 aatactaacg ctagcgccaa accaccagcc attaaaatgg caatggagat ttctgaatag
    36841 gttcggtaga tcgttcgcag atactccaat atcacagctg ctaatacaac gactagcatg
    36901 gtcgtaagtg taggattaag gttcaaaaag aatcctaacg ctacccctgc taaagagaca
    36961 tgagaaagcg tatcagccat cagcgattgt cttctgatca ctaaaaacac acctagcatc
    37021 ggggcgattc cggcaatgaa gatagcggca agaaacgctc ttctcatgaa ttcatatgaa
    37081 agcaacgcca ttgagaatcc tcctttctca ccagtcggat ctgtctgtcc gcatactgtt
    37141 tgatatcttc atgatcatgg gtgatcatta gaactgattt cccatggaca tgcgcactat
    37201 gtcggagaag acgataaaat tcatttctgg attcttcatc catacctgtt gttggttcat
    37261 caagaacaaa taagtcaggg tcagtagcaa aaatacgcgc taaactgatt cgctgctttt
    37321 gtccgccaga caattcacct attcgccgat ttcgcatatc ccacatccct acagcttcta
    37381 aagcacgctg tacatgttga tgatcttttt cagagagacg cttgaaccag cggcctcttg
    37441 gatatcttcc tgaacggact aattcgatca ccgtactagg aaaacctgca ttgaaagatg
    37501 caacttgttg aggaatataa ccgatactta atttttttcc ttcttgattt ttctcagcaa
    37561 tctttatcat tccttttgaa ggtttcagta atcctaacga tgctttcacc aaggttgact
    37621 tggctgctcc attttctcca gttaaaatga caaattcacc cgcatctaca tgataagaga
    37681 catcttctag tacaggttca tcatcatagt aaaaggtcaa atcagatatt tcgatataac
    37741 gcaccttaca acctccattc taaaacttaa ttgatacttt cttttaaagc tttcagattt
    37801 ttttccatga cagaaatata attttcgcct gctttgattt gttcattagt caagctttct
    37861 aaaggattca acacttctag cttcactcct gtttcattcg acaatgtctc agctaccttc
    37921 gaagaggcat tttcttcaaa ataaatcact ttgatgttgt ttttcttcac aaattctttt
    37981 agctcagcta agcgacttgg tgtgggttcc tgatctggtg aaacgcccga aatcgctact
    38041 tgttttaagt cgtattgatt cgctaaataa gcaaaagcag catgctgagt gacaaatgtg
    38101 cggtttgttg catcttttag ctcatcggag tatttttgat ccagtgcttc aagctttttg
    38161 atataagcat cggcattctt cgtaaaagtt tcctttttat ctggatattt ttcgcctaat
    38221 tcttcagcaa tcgttctgac ttcttttata gctaataccg gatcgagcca aacatgtgga
    38281 tcgtattcat gtgaatgacc atgcccttct tctgattctt catgttcgtc tgcttcttgc
    38341 gattctaaac ggtcgattcc tttagctgct tcgatcatca aggtctgttt gctatccact
    38401 gaatccttta acgaatccac aaaaaattcc atgtctgaac tattatacac gaaaacatct
    38461 gcatcggaaa tttctgctct ttcttttgca cttggttcga aatcatgcgg ctccgttcca
    38521 gcagggatca aaagtttcac gtttccttca tcccctacga tctcttttgt aaaatcatac
    38581 atcggataaa aagtcgtaac gatcgtcaaa tcctcttttt tgtctgcttc cttgttcgta
    38641 tttccacatg ctgcaaacag taaagcacct atcattaccg tagctgtcaa aagatatttt
    38701 ttcatttata tgctcctttc aatgaacaaa tcggaatttt acgatttatt aacgtgaact
    38761 aatgtatcaa aataaaaaaa cacagtcaat agaaatccat caatttctga tttaagcatg
    38821 tatattcttg attaaatcgt aaaatggatg agttgctgct catatctagc catttcacta
    38881 caataaaaaa ctgaagaata ccattttagg attcctcagt ttttcatcac ctattttact
    38941 tcttatattt tattgcaggg ttcgtactaa ataaacttcc tcacaaaaac cttttaaccc
    39001 attatatact ctctgcgctc ttgaataatg ttgacacaag gcaaaaacgg ttggtccgct
    39061 tccactcatc aatgcagcat ctgctccgta cttgatcata cgctctttga tttgatggat
    39121 gactggatgc cgctttcccg ttatatcttc taaactgttt cccatttttt tgatcatctg
    39181 ctgataatct tgctctcgga tggcttcagc aagtccgtgg atgtcttgat gttggatggc
    39241 ttgcgtatct acttctctaa acactgtacg tgtagagaca ctcattcttg gtttgaccaa
    39301 cacaacccag cactgtggca tagaaggtaa tacttggatt tcttcccctt tgccaccgac
    39361 aaatgccgta cttccgtaga cacagtaagg aacatctgta ccgacttcca aaccgatttc
    39421 tgccagttct tctaacgaac agcccagatt ccataattgg ttgatccctc ttagagcagc
    39481 tgcacagtct gtacttcctc cgcctaatcc agctgctact ggaatattct tcttaacaaa
    39541 gatcttgaca ccggttttga tattgtattt attttttagc aataaagcag cttgataaac
    39601 attgttccgt tcatctaccg gtaaaaatgc tttatttgtt tctacaatga ttttgtcttc
    39661 tgttatttct tcaaaaaata gatgatctgc caagtctacg ctcgacatca ccatttccaa
    39721 ctcatgatac ccatcttgcc gtctatataa tacatccagt cctaaattta tcttggctgg
    39781 cgctttttca atcaattcca tcctctcacc cattcgaaaa gtctaagtcg caagtattct
    39841 atcataggga aaaataaaag tatagcttat gtctgcctag tggttgattt tgtcatcgtt
    39901 tcaacagctt tcacagaatt ttctcttcat aatccaaaca agttctagtg attcttataa
    39961 ttctgtgagc acttcttctg ccaaactcgg aacatttcct ttttcttttc ctatcagact
    40021 gagtagctgc atggtagcaa atccgaaatt acttcctttc ctataagcaa aataacgtcc
    40081 ttcccaatga tcgacatatt ttaatttctt ttcataagca gactggaagg aatagaaaaa
    40141 attaccataa ttatatacga tatgcatgac tttttcttga aagaaagaaa aatctgtttc
    40201 tccaacatta gctaatggag ctaagcctag attggcttcc gagtagccca ttttctcgta
    40261 tacaataaac agattggcta acagatagtc agctaattcc tcagattctt taggtaagac
    40321 ccgcaacaaa tcatatcccg cctgcttctt cgtcatcggc tgagcggtga tgaagccaac
    40381 gactttttga tctttacgga caataccgac accgctagat aatacatatg atttatcaaa
    40441 tcgtcccaca gagaagtatt tttctcgtgc accttgaagc catttttgtg aaacttcatt
    40501 tacagcttga tgtaattctt ccggcagttg atcgtaccaa gtaaaatggt atccttcttt
    40561 catcaaacgt tgatattcaa gttgctgact cagccgagac aagtcttctg ttgctagatc
    40621 aacaatccct gcttcaccta ctttcgtgaa gtgaaaaccc aattcatgaa gcatcactac
    40681 gtagctttct gatatcttat aaaacgcaag ctgatagcct aaacaatctg cttgtttcat
    40741 aaactgattc gttgctgccc gcatcatttt aggattgcca actggatctc ctaaaacaaa
    40801 gcatttgttt cctttgatct gataaccaaa aacgacttga tcttggtcgt cttcttggta
    40861 ataaaaataa ctgtaacctg acaagcgaag ctgatggctt gtagctgtgc ctccgtatgt
    40921 ttccaataaa cggtcaaaac ggtctttttc gtactcactg ccaagcttcg tagtatcttc
    40981 agctaaatat tgataaagcg ccactaaagc tagcaatgag atgcccaaac caataagccc
    41041 tgagaaccac acatcatctg aaggaaacaa aataaaatga tttcctgtta ttcgaccgac
    41101 taaaaagcgt ccgctgtgat agcctgcaat tgcgtaaacg ataaacaaaa agccgaacaa
    41161 gctagcatca aagattatag ctccccaaga ataaacgaat tttgttcgat aaaattcttt
    41221 tctggataac cacacagcta acagaatcaa gacatatacc aatatcaact gccaagataa
    41281 cgtacgtgtg attgtattta aaataccaaa agatagtaaa gcaatcgtag gcagaaatgc
    41341 ctttttgact tttactgaaa gtgctctagc aaggccgagt aataaaaagc caaccaatag
    41401 attcactgtc tgatctaaaa aattgaatga gaacggcaat aatacttgaa atagccgact
    41461 gattgcagac agattagtga ttgttgatag aagtaccatc atgatccccg caaaatagac
    41521 agcacttacg agcacgatat gcgcaacctt ttggaaaaac agtcgcggaa tgttatctaa
    41581 aaatcgattg attttgactc ctgtatgtgt gatgaacaat agaagtcccg tcataaaagg
    41641 tagaacataa taaaacaacc gataatataa tagccaaact actgctacat cttgccgaac
    41701 agacaattga ctcattccta agatcatcaa aacgtcaaaa gtccccattc cacctgggac
    41761 catcgtcagc attcctatca atgtagcaat catgaacata ggatagatcg aaagcatcga
    41821 aatatctatt ctcatcaact tgccgatgat caaaaatacc gcaagggctc ccgtccattg
    41881 tccaaaggat gccagtagca gaccaagcaa gccttttgga taaaactcga agaacaatgt
    41941 ctttctcttt agataagcaa aaaagaaaat gactggtgct aaaaaactac cgccgaaaag
    42001 ccagatccaa tagcttcgaa aaatactttc tgactgaata aaaaagacat caataaatgt
    42061 aagaaacgac catacagaca aaccagtcag cataaatagt gcgacttttg atacagtggc
    42121 taaaaccatt tttctatttg tctgctttcc ataaaaacta gcccgcagac ttgctcctac
    42181 cacaccacca aaaccggcga gattgttgat cgtgtttgtt acccacgcag caagaagaaa
    42241 ctcttttctt tccatttttg gttttccttg tttttctaag actctgatcg tcacccaatc
    42301 atacaacaac atcggagtga cacccagcag tccagccaga atcatgagca ggatcgtcat
    42361 gcggctttgt tgtcccatcc tttgccaaat atcttgccaa ctcatgccat tagcaatatt
    42421 tgcgacttga tttgccacaa agactaacac ggagccgaaa aaaatcagtt ttaaaatcag
    42481 actatgttcc tttatccatt gcagcatcaa gtatcgttta tttttcacat tttcccattc
    42541 ctaacttgta tacttttgtg tcatacccca atgttatctt atttatttta atttttccac
    42601 tttagacctg ttctttgctt gcgatgatag aaaaagaaag atatcctaat gctaaaggag
    42661 aggataacat gaaagtagga tttatcggca cagggaatat ggcacaagct attattgatg
    42721 gtttattaaa aaaaggaaca gtctcgaaag aagaggtgtt cgtttccagt ggacattttg
    42781 aaaatgcaca aaaatttgct gctgaaactg gcgtccatgc atgcaaaagt aatcaggaag
    42841 ttgcatcaca atcagatatc gttcttttag ccgtgaaacc aaaaatcatt gaaaaagtat
    42901 tggaagaact aagagaatat agccagaaag tcttattcat ttctattgct tcaggaaaat
    42961 cattaataga tttagaaaca gcgcttgaag atcctcaggc ctccatcgta cgtgtgatgc
    43021 cgaatgtcaa tgtcagcgta ggagcaggcg tttctgcgat ttgcaaaaat gaagctgcca
    43081 cggaggaaca gcttcagcaa gctacgatga tttttgaagc aatcggcagc gtctatcctt
    43141 tagcagaatc agatttcagt acattcattg gccttgctgg cagctcaccc gcattcgttt
    43201 acatgctgat cgatgcgatg ggacggactg gtgtactgaa tggtttgtcc aaacagacag
    43261 ccactgagat tgctgcaaaa gcaattatgg gttcttgtca aaaattcctt tcaagtgata
    43321 aaaatccatg ggaacttgta gaccaagttt cttctcctgg cggtacaact gttgcaggtg
    43381 tagttgcctt agaagaagca ggattcatcc cagcaatcat caaaggaatc aatacgacga
    43441 tcgagaaaga tcttgagatg ttgaataaaa aataatgata gcaatgtttt ttcgtgcctt
    43501 acaaaaaaag aagagctgtg gcttttgccg cggctcttct tttgtatttt ttattattta
    43561 gcataatcag ttgcacgagt ttcccgaata acgattactt tgatgtgtcc aggataatcc
    43621 agttcttctt cgattcgttt acgaatatca cgtactaaac gaacagcgtc taaatccgag
    43681 atttcttctg gcttgaccat tacccgaact tcgcgtccag cttgaactgc gtaacttgat
    43741 tctacaccgg cgaagctgtt cgaaatattt tccagacttc ttaaacgacg gatgtagttt
    43801 tctaatgatt cacttcgcgc accaggtctt gctgctgaga gcgcatctgc tgcagctaca
    43861 aggacagaga taacagaagt agcttccaca tctccatgat gagaagcgat cgcgttgatt
    43921 actactgggt tttctttgta cttggcagct aattccgcac cgatctcaac gtgagatcct
    43981 tcaatctcat ggtcaagtgc tttcccaata tcatggagca acccggcacg ttttgctaat
    44041 tggatatctt cgccaagctc tgctgctaag ataccagcta attttgcaac ctcgaccgaa
    44101 tgcttcaaga cgttttgtcc ataactagtt ctgaatcgca gacgtccgat gatcttgatt
    44161 agatctggat gaagtgtatg agcaccgact tcaaacgctg cctcttcccc gtattcgcga
    44221 atacgttcat ccatttcttt acgagatttc tcgaccattt cttcgatacg cgctggatgg
    44281 attcgtccat cttgtatcag tttttccaag gtcatccgtg cgatttctct tcggattgga
    44341 tcgaatccgg aaagaacaac tgcttccggt gtatcatcga tgattaaatc gataccagtc
    44401 agcgtctcaa gcgttcgaat attacgacct tctcgtccaa tgatacgacc tttcatttca
    44461 tcattcggaa gggtaacgac tgaaacagtc gtttctgaaa cttgatctgc tgcgcagcgt
    44521 tgaatcgcta atgataataa gtttttcgct ttgcgatctg cttcttcttt ggcacgctgt
    44581 tcagattctt ttaccattag agttaactca tgattcagct cttcttcagt cgatttcatg
    44641 atgacatctc gtgcgtcctc ttttgataaa ccagcaattt tttctagttc tttttgttga
    44701 tcttcaatca ttttttcaac gtctttttct cgctcttcaa ttaattgctg ttttgaacta
    44761 agtgcttctt ctttactttc aagtgagttc tcgcgttttt ctaacgagtc atctttacga
    44821 ttcagcgttt gttcgcgttg aatcaaacga ttctcttgag attttaattc taacttactt
    44881 tctctcagct cactttcaac ttcagaacgg tatttctgat tttcttcctt agcttctaac
    44941 aatgcttctt tttttagagt ttctgcttct ttttttgcac tatcgatgat tccagcagct
    45001 gatgattgtg caccagctat ttctttctcg tgacgtgact ttgcgaccat aaaccctaaa
    45061 ccaagaccga caattaaacc gatgatagcg aggagaatgt tgaataccat aaaatccacc
    45121 tccatactat ctttttcatt tttatcatct atacagcttc tatgataaat tattctaaac
    45181 tcgtcaatat gcatcttcgc atatatgtgt acaaaaaaca tgcacaactt tattctaatg
    45241 tttgtacccc gtagtgtcaa ctttaaataa acaaagaaac ggctttctac ttattcatta
    45301 aacgatggtt tttaaaataa atgacactag tttcatatag gtaaaccttt aaattgagaa
    45361 acttttctat taaattattc tttgtagatg aacagatatt tttattttat gtacccactc
    45421 gtataattaa ataactcacg atttctttat tcaataaaaa aacggcaggt caaggagagt
    45481 acctactctt cagaccagcc gtttttgttt atcataattt ttattcttct actaaaggca
    45541 attcttcttg tcctttttca tcttctggga cagcgacttc ttcaccaata ccataagctg
    45601 ctctaactag agcagaaact tctgccatca tttcaggatg attagccata tagattttag
    45661 cgttttcccg tccttgacca atccgatctt ctttataaga ataccaagca ccgcttttat
    45721 caacaatgtc tttttctact gccatgtcta gaagttctcc ttcttgtgag atccctaaac
    45781 catacatgac atctacttca gcgattttga aaggaggtgc tactttgttt tttacgactt
    45841 tgatctttgt acggttacca acaatatctg taccttgttt caattgttct gcacgacgta
    45901 cttccaaacg gatcgttgcg tagaatttca atgcgcgtcc tccaggagtg atttctggat
    45961 tcccaaacat cacgcctact ttttcacgga tttggttgat gaaaatagcg attgtttttg
    46021 ttttgttgat cgaaccagag agtttacgca atgcttgaga catcaaacgt gcttgtaacc
    46081 cgacatgtga gtcacccatt tcgccgtcga tttctgctcg tggaactaaa gcagcaactg
    46141 aatcgactac tacgatatct acagccccac ttgatactaa agcatcagcg atctctagac
    46201 cttgttctcc tgtgtcaggc tgtgaaagaa gtagttcatc gatattcaca cctaattttt
    46261 gtgcatattg cggatctaac gcatgctcag catcaatgaa agcggccgtt ccgccatttt
    46321 tttgtacttc tgcaatagcg tgtagtgcaa ctgttgtttt acctgaactt tcaggtccat
    46381 atacttcgat gatacgtcca cgggggtaac cgccgacacc taaagcgaca tctaacgcaa
    46441 gagagccact tggaattgta gagatttgtt ggtcgatttt ttcgcccaat ttcataatag
    46501 agcctttacc atagctcttt tcaatttttt tcagtgcagc atctaaggct gctttacgat
    46561 catctgccaa tacgataatc ctccttatat ttacaaatca gagcaacatt tcattatcct
    46621 tatcatatct tttttagtga aaaaaagcaa gaaaaaaccg aacaaatatt cgcatttatc
    46681 attttcttaa aagctgtttt ctcaaaagat caaatccttg cataaccgca ttatatctga
    46741 tagcagcacg atctctggca aagtgatatt tttccgaaac cacgccgtct ttagatgaaa
    46801 tagcgatcca tactgttcct gcagattgac cttctaacgt gtccggtcca gccactccgg
    46861 tgaaagctac actgtaatct gttcttgcta tctcacgtgc ttgaatggcc atctgctcca
    46921 cacattcttt gctgactgtt ccatattctg ctaatagctc cggatcaatc tccagaaaac
    46981 cagcttttgt ttggagcgag taggtaacga atccgcctgg aaatacttca gatacaccag
    47041 caatgtcgcc taatgcggct tggaaggcgc cggctgtcaa actttccgct gctgttacag
    47101 tctgcttgtt ttctttcagt agttccacta cgacttttgc tagcgaatta tcatcgccgt
    47161 atccataaaa atattcccct actcgttctt ggattttttc ttctaatgct aaaagagctt
    47221 gattccctgc ctgaacatca tttgttttca ctgtcagtcg taatgtcact tcatttggtt
    47281 tggcatatgg tgcgatcgtt ggattgatct gtgtctcaat cagatctttt agctcagtga
    47341 ccagtcgcga ttcaccaatc ccataaaagc gcaaaacttt agaaattagt ttttcttcag
    47401 aaggaaaacg ttcctctagt aaaggacgta cttgttccac aaacattggt ttcaactcat
    47461 taggcggacc gggaagaagg atatacgcat gtgtatctgt ttgataaaaa gtccctaaag
    47521 caagccctgt acgatttggt agaggaaccc cgccttcgat aatctgggac tgttgcagat
    47581 tgtttttggt catttttcga tgggttgttt caaaatatgc caataatttt ttgtatcctt
    47641 ccgtattttg tatgagagac ttaccgaggt gagccgcagt tgcttctttt gtcaggtcat
    47701 cttctgtcgg tcctaatccg ccacataaga gtatgagttc acttcttgtt tcagctagag
    47761 caatcaactc ttctaaacgc tgctgattat ctcctacaac tgtttgataa taaacttcga
    47821 tacctagatc agctagctgt tctgacaaga aagtggcatt cgtattcacg acttgtccta
    47881 gtaaaagttc tgttccgaca gctattattt ctgctttcat tacttcccgc tcccttatat
    47941 agtatacgct gtatttttat atttttttat ttaaaaagag ggcaaggagc tgtgacaaaa
    48001 atcttgtcac agctccttgt tccaaataaa cagtgttctc ctgccaaaac ctcggtcata
    48061 agtcacaata tacataaaac atgaaaagct gttttttgaa attatccctt gtgactcaga
    48121 gttagacggc aggctcacaa cctctccact aaacgatact tacattgatc ctttgaacac
    48181 accgctgttt ttgatgaagt aatccacacc tgaatagata gtaaagatca gacaagcata
    48241 aagcatgatt tgatctactg gaataccgat caggctgaat gggatattat tgataaacag
    48301 caagataata gcaaccattt gtgtagccgt cttgactttt cccggccaag ctgcagccat
    48361 gacttctcca tgttcgacaa gtagtaaacg caagcctgtg acggctaatt cacgacagac
    48421 aatgatcgct actacccaac caggggcctt tccttggtca accaacatga taaaagcagt
    48481 cataactagc attttatctg ccaacgggtc cgcaaacttg ccaaaattag tgacaaggcc
    48541 tcgcgcacgg gcaatttttc catctaacca gtctgtgata ctagctaaag caaaaataac
    48601 tgcacctact agctgtgtga cagccagtgt ggtgtctcct acgttaaatt gtccccaatc
    48661 taaaggtaaa cctacaacca aaatgaaaat cgggatcata caaattctaa gtacagttaa
    48721 tttatttggt aaattcacac tatcgactcc tttcttccct attctacgtg actatcagcg
    48781 aagtttccac cgatattatt tacttttcca aatgaattta ttcaaaggag tatttttatt
    48841 gaaattgatc tttttgaatt aacgaagaag gaccgcgaca cagatttgtc acgatccttc
    48901 ttttttatac ctacaagtaa acagatgcgt aaactaattt atacgatcat tctgcgtatt
    48961 gaagagtaag gttcacattt tttggactct gattgttttc ccctggatta actggtacgg
    49021 attctccatt cgctttgatc gttgcattgc ttgctgcacc aatcgtgatg gtcgcatttg
    49081 ttgctgtttc cggtaacacg gtactctgtg tttcatttgc tgccagtgtc ccttgataaa
    49141 cataggcatt gtctactagc acacctaacc atacacgatt gacagcagta aattcaaggg
    49201 ttacaggctt ggtagcgtct gtgatattta ctgcgatggc actgccagaa tcttctcctg
    49261 tagctattgc cattttcttt tcttcacttg attttgttgt tgattctact gtagaagact
    49321 ctttggtctg cgtgctttgc tctgaggaag aggactcctc gactgatcct tctactttaa
    49381 gcgaagaagc tgtgtctccg attattggat cagcttgacg atcttgccaa gtcatatagc
    49441 ccacgacaac gacaatcgcc aaagcaacca atcctaataa aatgacagga agacttgtcc
    49501 aaaagcgact tggatgcgat tcttcctgat gcattgcttt tctagaagta cttactgttt
    49561 caggctgcgg acgttcaggg accgatcctg tcaatttgct tttcccatca aaaacatcga
    49621 ctaaatggtc tccatcttct cctacagctt cagcgtattg ccgaataaat gcccgcacgt
    49681 aaaatttacc aggcaattgg tcaaaatcat cattgtcgat tgcttccaaa taacgttttt
    49741 gaatttttgt aatctgctgt aattcatcta aagatatttt tttattcagg cgtgcttgcc
    49801 gtaatttttc tccgatggtt tcaaaagcca ctcgttcatc ttctccagtc tgtttgattc
    49861 tccgaagcat ctgctgttgc aacgaaagac gttatgtttg ggtgctgtga caaaagtcat
    49921 gtcacagctc cttttttccg aataaacatt gttctcctgc caactcctcg gtcacaagtc
    49981 acaatatcca ataaatataa gaagctgttt tttgaacttg ttccttgtga ctcggagtta
    50041 aacggcaggc tcacgacctc tttcttaaac ggtgttcaat cagctgttcc tcggtattag
    50101 ctcaaaaacc ggcaaaatat aaggagatat ttttccgtat gtttctctaa tactcaggtc
    50161 aaaacgctga tttcacaacc tcttccttaa acggtgttca aaagcttgca tctgcggtaa
    50221 taagtccaac caagttaaaa acattaagaa gctgttttta cttggctgtt cttattactt
    50281 gatgcagggc agctttttca caacctcttg gtacacggtg ttccagaagt agcttcttcg
    50341 gcaataagcc gaaatatcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt
    50401 cttgaagctg accacttctg tcacaacctc tttgattact tcgtttgatt ttattttaat
    50461 ataagttgat taaaagagag catgatttgt tttattttct cactaatttt tccaacacaa
    50521 ctaagaatac cataaaagct gattttggag gaggttccta ctacaagtct aaaacgaaat
    50581 ttaagacttt tcctcataaa atactggttt attttggtct catgtaaaac tcgctgaagg
    50641 cctcttcact tataaatgct tccccagctg ccagtacgtc tttcatttgg atcgaatcga
    50701 tgatttcagg aagatcaaat aaagttcgat cgccaaataa actttgcgta aattgattgg
    50761 caatatattc gatcgaattt aatgattgga aatattgtcc aagcattttc ttcttcagca
    50821 aatcgagatt tgtttctgac acttccggat catcagcgaa tccaagaatg atttctttga
    50881 ctttttctgc tgctttctct ggttcgtccg tatctccgct gaagtccgca aagtgaaatt
    50941 cacgatccaa gctgaattca aaaccaaagc tgtcatcgat aattccttga ttgtacatcg
    51001 ctagatagtt tcgagaagta ttgcctaaaa tcatttggaa caacagattg attgctgttt
    51061 tatagcgtaa cagttctgtt ccttcttgcg gcaatgtgtc taatccttta ataccaagta
    51121 caaatttatc tcgtgtaatg gctgcttcca atgcactttg tttgatgatt tcttttgtgt
    51181 tttcagggaa ataacgtaca atttcttgct ttggagggaa attttttgct tcttggtttt
    51241 cccgaataag tttcatcaat ttctcaggct ccatcttacc aactacaaat aaaaccatat
    51301 tgcttggctg ataaaacgtc cgatagcacg tatacaagtc ttgagctgtg attttgtcga
    51361 tactttcaac cgtgcccgca atatcgatat gcaatgggtg tgtaggatac aagttattta
    51421 gtatgccaaa aaacatccgc cagttagggt cgtcttcata catttggatt tcctgaccga
    51481 tgattccttt ttccttgttt acagtttctt ctgtgaagta aggtgcttgt acaaaatcga
    51541 tcagagtcgt cagattcttt tctacctgat ccgtcgtaga aaacagatag ctggttttcg
    51601 taaaacttgt gaaagcattg gcggaagctc cttgtttccc aaataattga aagacatcac
    51661 catcttcttt ttcaaacaac ttatgttcta aaaaatgggc aattccatct ggaactttca
    51721 ccttttcttt ttcaccatag ggaataaatt cgttatcgat cgaaccataa ttcgtactga
    51781 acaaaccata tgtcttgtga tagtcatttt taggcaacaa atacacggtc aaaccgtttg
    51841 gcagtacttc atgataaagt gtttcattga tttgctcata ttctgtttta ttcatttgca
    51901 tttcctcctg ctaaaaagaa gattgcctgc aattcgattt gctcagctac ttgttgtatc
    51961 tctttgatcg ttacgctctc catacgtttc aaccattctt catcagataa ttttgtttcc
    52021 ggcagccagc tgtccaaaaa agaagcttcg attgccgctt gcggagagtc tagagaaagc
    52081 agatattggt ttctcagcat tgcttttgtc tgagacagtt ccaactctgt tatttcacca
    52141 tttcgcaaac tttctaattg ctcgtgaatc agatgaagta cttgatttcg gttcttttca
    52201 tcgataccgg tttggacact catgaatcct cggaacgtat ccacgctact agaggcataa
    52261 taagctagac tttctttctc tctgacattc atgaaaagtt tagagtgagg aaaaccgcca
    52321 aataaaccgt taaagaccat tagcgcaaaa cgttctggtt catcgtaata cacatttgtc
    52381 tgataagcta ggttcagctt tgcttgaacg attggctcac gtacttgtcg ttccttcacg
    52441 atattcactt gaggttgggt ataaaacaga tctggataaa ctgtttcccg cgactcaaat
    52501 ggtaattgcc caactgcttc agctgctttg tccggatcaa catcgccgac aacaaaaatg
    52561 tcgatttgat catttgccat catttcttga tagtaggacg ctagcgaact agcagtgatc
    52621 ttgtccaaat cttcgactac gcccaaccct gggatctttt gttccgggct gttttcaaaa
    52681 taaagttctt gcaaggcata ggaagcaaaa gtttgtttat cttccttcaa actttccaaa
    52741 tatagacgca gattgttctt ttccaagtca aacgtcaact gatcaaactg atgattttta
    52801 atatttggat aaaataaaac ctcttttaaa aaatctaccg cttgagaaaa aaggtttgga
    52861 tcattgacat attcaccatt cacaaaactg atccctgcgt ttagccaatg taaatttcct
    52921 ttttttctaa ctgataatcc aaagcttgcg ccataaagct ctgcaagttg cgcacttaac
    52981 tttgtctgat ccgaataatt caaactattt gtttccaaca tgctgctgag aagtgaacgt
    53041 ttcgtgatga cttcttgttg cagacgcgca gtaaaacgga taaaaatccg gatcgttttg
    53101 tattgttttg ttggtaagac atgaagattc acgccttctg ccaattgata caccatattt
    53161 atatgctcct tttcacagtt gattgcccgc ttactctttt aatgggtcaa cctgcctttt
    53221 ttacttattc ctctgcttga aatttataag ttcaggcaat tcgccgtttt ttctaaatga
    53281 tgttacactt tatagtatag catagatgtt ttttatcaaa agaaacagac ggctaaaagc
    53341 gtatattgtt tttttatttt ttatttttcg atatactaaa gacaaataaa agaagggagg
    53401 gtcattcatg atcaaagaat tcaaagagtt catcatgcgt ggagatgtct tggatcttgc
    53461 tgtcggcgtt gttattggta gtgcgtttac aagcatcgta aaccaagtgg tgcaaggctt
    53521 gatcacacca ctaataggtt ggtttgtcgc aatgatcaca ggtacttctg atttagaagg
    53581 agcactttca attcttgact ggtcgccgac aaaaggcgtc acatttgcgt ttggtaacgt
    53641 tattagtgct atcattacat ttctgatcac tggttttgtc ttattcttag ttgtcaaagc
    53701 ggcaaaccgt gcgaagaatt tacgctccaa agaagagcaa gtggaagtgg aagaaactcc
    53761 tactgcagaa gattatctta gtgacatacg cgatcttctt gccaaacaag tggcagaaga
    53821 caatacacat ccagtagagc aaacaaatct ttccgatact tctaaataat aaagaatcta
    53881 aaaaaaaaga aagccgattt tcagtgtcgg ctttcttttt ttagattctt aacttgctac
    53941 ttgttccttt ttcatgtcga tatgtggaat atcatcttcc aaatacacat ctgagattgc
    54001 cttgaatcca aacgactcat aaaaagcttg taggtattct tgtgcgccga tcacgattgg
    54061 tttttctgga aaatttttct ggatcatgtt aatcgtttct tgaacaatct ttcttcctaa
    54121 accatcttta cggttgtcct gatgaacaag gacacgtcca aaatggatac tctccgcttc
    54181 ttcatatact cgcgtgtagg caaggatctt cccatcagta tcttgaaaaa acgtatgcca
    54241 agcttctcca tcaatgtcat caacttcttg ataaggacac tgttgttcca caacaaaaac
    54301 tgcgattcgt tctttgacga tcgcgtaaaa ctctgctgtt gacaattctt caaatttctt
    54361 tacctgatat tccactaagc aaacgccccc tgctttttta ttcttagcat accgattcgc
    54421 acacaaaaaa gcaaacgaac cttacgaatc tcaggctcgt ttgcttttta aactgcttca
    54481 gttaaaaatt cgatctcaac tgtccgagta agtacatctg aatagctgta agatactcgt
    54541 tcaaaagaat tttcttcagg atccaaatca acaacgaata ctgaaggata ggtttctgtt
    54601 aaaacgccgc ggcgttctgt ttgacgcttt cttcctgtct gagcaaccaa catgattggg
    54661 ctgccgatac gaccttctaa atctttcttg attgaagcta aagttgttgg catacagttc
    54721 acctctacta tcaactatac cacatatttt taaaattttc aagtttacca taaaaaaaaa
    54781 agaaatgcaa taaaaataaa taagaagctc attaagattt ttctaaatta ttcctcatta
    54841 taaaatttca agtgtttttt tattttgagt tttactctcc cgtaagcgct cattacttgc
    54901 ttgtgatcca ttgctaaaca ttcagccatg tctacaaaat caacgccttg catataataa
    54961 tgaaaaattt tagcttctat ttttgaaaac tggatttcgc aattttcgat tgcttctttt
    55021 aataaaagat gctgaattgc taaaggttct tgcaatgtga tctctgctaa aaagtctggt
    55081 ccttctgtct ctattttttg gtcgattgag acttcatctt tttgatcacg acgcttcagc
    55141 gcgttctgct tcctcaacag agaatagatt tgattgctta taacgctttt ataataactt
    55201 ccaaagctaa cacctttgct tttttcgtaa gttctcaatg cctgatataa agcgatcctt
    55261 gcttcttgtt cccaatcctc agaaacataa tttcttaaat aataggtgtt tttcaaacgt
    55321 agtaaaagtg gaaaatattt ttgatacagg gcagcaaatg cttcttcgtc accattacgt
    55381 gcagcgcgca ataactcctc agacataaaa taattcctcc ataaattcgt ttatggagga
    55441 atcttatcat caatgattca tcgaactgat tagaacgata tgttatttat tttttctcat
    55501 taaatcgtcc aatttttggg acaatttttt taactgctct tcattccatg gagaattacg
    55561 tcggaaatca ataaaatgca tatctttatt ttcttgggct attgtttttt ccgttttttt
    55621 tatcgtctta tataattcat aagcagaagt tcgtaatgct ccttgggaaa agatcatcca
    55681 ctgttctgct aaatcgcttg tcgccactgt cacttgtgtc aaacggtcat tcagctgacc
    55741 agcgattctc tcgatataac tgtcagccgt ttcgtcttct ttcgtaaaaa caacatccaa
    55801 ctggtatttc ttataatttt gttggatgcc cggaactagt tgggcatcga agacaacgat
    55861 gacttccatt ccttcatatt tcgcataatt tgataaacga tgtaaaagag cttctcttgc
    55921 atccgccatt ttgtcctgct ttttaagctg gacaagttcc ggccacgaac caatcatgtt
    55981 gtagccgtct acaatcaaca gctgtttttt catggtctac tccccctgac gttgggaaaa
    56041 agcctgatac ataagcaaac cagcagcaac actggcattt aagctctgga cgtgcccggt
    56101 catcgggatc gtcagcaatt catccatttc ttttttcaat cctggactca ttcctttgcc
    56161 ttcattacca atgactaaag caattgatcc tttggcattc catttttgga agtttgtgcc
    56221 ggtcatatct gtgccaaata cccaaaatcc tgtttctttg agctgttgca cagcttgtac
    56281 caaattcgtc acgcgtgcaa cagggacatg ttcgactgcc ccagtggaag tctttgttac
    56341 gataggtgtg attccaactg ctcggtgttt tggtatgatc acaccatcga cgccacttgc
    56401 gtctgctgtc cgtaaaatcg acccaaaatt gtgtggatct tcgatgctat ccaaaatcaa
    56461 aaagaagggg tcttcttttt tctcttttgt tcgttcaatc agttggtcaa gggataaata
    56521 ttcataaggt gtaatcgcaa gaacgactcc ttgatgcacg ccgtgatcgc tcaaagtttc
    56581 taatttttgt ttaggaaccc atttgactgg cacagcttgt tcacgtgcga tttctttcag
    56641 ttcttctact ttttctcctc gtgaatcttc ttgaagaaag agtttattcc ctcgcccttg
    56701 ttgtagcgct tctactgcgg catggtgccc aaagacgaaa ttatcttcta atgcttcttc
    56761 attttgatca gaaactgact gatttcgtcc agtttgcttt ttatttgaat atctattttg
    56821 ttttttgtct tcaaatccac gtttcgattg atgtttgttt gttctttctt tacccatttt
    56881 tttcaccaac tttctgaata caccagcgga tcaattcttc catccgctcg gtttgttttg
    56941 tcaaatgcag atagcccatg agcgcttcga accctgttgc gacccgatac gtcgtaacat
    57001 cagcattttt tgcgctcgta tggcttttag catttcttcc tctgcgataa taaagttctt
    57061 cttcttctgt caaaagatgt tcttccagca tttgctgaat caaaaaggct tgtgcattcg
    57121 cagaaacata attcgttgcc gcacgatgca gcttattggg ttttgtttct ccttgatcaa
    57181 tcaggtggtc acgaacatag atttcgtaaa tcgcatctcc aacataagcc aaggctaaac
    57241 cgttcaattg tgtgtaatct ctcatgcttc tcttctccat cgggttcctt gcggtgtatc
    57301 ttctaataag atcccttttt ctttcaacaa atcacgaatt tcgtcgcttc ggacaaaatc
    57361 acgattgctt cttgcttgat ttcgttcttc gatcaattgt tcaatctctt catctaataa
    57421 ctcttctgct gtaaataaaa taccgaagat cgaaagccat tgcgagaata aagcttctcc
    57481 ttgtgccaag acagcggcag aaactttcgg ctggtctaga taagtgttca gccacttagc
    57541 aagctcatag accactgtga tcccatttgc tgcattaaaa tcatcgtcca tttcttcgat
    57601 gaatcgctgt tctaaatttt ctaacaaagc gattttatcc gcatcatctg ccaaattctc
    57661 tgaggcagtt tcttggcgga atttcaaatt gtcaaacgta ttcttcagtc gttgtaaatt
    57721 ggtggctgct tctttcatcg tcgtttcact gtagcggatc ggacgacggt attgtgtggt
    57781 cgccatgaag aaacgcaaga cttgtggatc aacgtttttg atcatttcat gtacagtgat
    57841 gaaattccct agagatttac tcattttttc atcgtcttcg ccaatcgtta catagccatt
    57901 gtgcatccag taattagcga attttttccc tgttttcgct tcactttgtg cgatttcatt
    57961 ttcatgatga ggaaattcta aatcttgccc gccaccatgg atatcgatcg tttctcctaa
    58021 atgttttgtt gccataacgg aacattcaat atgccaaccg ggacgtcctt tgccccatgg
    58081 tgaatcccaa gaaatctcgc caggttttgc ttgtttccat aaagcgaaat ctaatgggtc
    58141 ttctttttgc tgctgttctt cccctgtacg ctgacttgct ccgacttcca gttcatcgat
    58201 ggattggtca cttaatttcc cataattttc aaatttacgg gtacggtaat acacatcacc
    58261 tgaagattca taagcatagc ctttttcaac caacacttga ataaatgcta aaatatctgg
    58321 catatgatcc atgactcttg gatgcagtgt agcaggttcg acattcaacg ctttcgtatc
    58381 ttcttcaaat gctcggatga aacgatcagc aacttctggt gctgtaatac ctaattcgtt
    58441 tgctgtacgg atgattttat cgtctacatc cgtgaaattc gatacgaaat cgactttata
    58501 gcctcgatat tcaaaatagc gtcgaattgt atcgaaagca atggtgctac gagcatttcc
    58561 gatatggata taattataca cagtcggtcc gcagacatac ataccgacat gtcccttttg
    58621 tatagggatg aattcttctt tttttctggt caatgtattg tagattttta tcattctgat
    58681 ctcctcactt tttctccttt gattcgaacg acttttgctg gtactcctac cgctgtagcg
    58741 tcgtctggaa catctttcaa cacaacggca cctgcaccga ttttggcgtt ttttccgatc
    58801 gttaccggtc ctaatatctg cgcattcgca gagagcatgg ctccttgttt aacagttggg
    58861 tgtcttttac ccgtttcttt tcctgtgccg cctaatgtta cgccatggaa taaaatcaca
    58921 tcgtcctcga tttctgcggt ttcgccaatg acgattccca ttccatggtc gatgaacacg
    58981 ccagttccaa tcgttgctcc tggatggatc tcaacaccag ttaggaaacg ccagaattga
    59041 gcattgatct tcgccaataa aaagagccgg tgctgatata aaaaatgaga gaaacgatgc
    59101 caaaacaagg catgaaaacc agggtatgtc aaaacgatct cagctgtcga acgtgcagcc
    59161 ggatcgtttc tctttgctgc atcaatcgct cgggtaaacc aactcatgac tttccactcc
    59221 tttcgtaaga attttatgta ggaaatcatc ttcttataaa gaaaagaagc gccttttaaa
    59281 taagtttcct tatcaaaaga cgctttccgc gtggttccac ttttcttcat aatcaatgat
    59341 tatctctgac agctaacgcc tgttatacgt tcttggctac tttgtttcct ataaaacagt
    59401 ttttcctgtt ttctgtgttt cgccaagacc actcccagag gcatttcggc aaccattcat
    59461 tgataccttg caccacccgg tatctctctt gtaatgatca gctgcttact tttttctgtt
    59521 catcgtgttt agttcagtac ttgatttaaa tgtgctaatg atttttcacg accaagcaat
    59581 tcgatcgttt caggcaattc tggaccatgc atttgtcctg atacagctac acggattggc
    59641 atgaaaagat tttttccttt tacgccagtt tctttttgaa cagctttgat ggcagctttg
    59701 acagttggag catctactac ttccatttgt tctagctgtg ctttgaatgc gtggagaaca
    59761 gtaggaactg tttcaccagc aagtacttct tttgctgctt cattcaatac aggatgttca
    59821 ttgaaaaata gcgtagacag atcaacgatt tctgcagcat agctcatttg aggttgatac
    59881 aggctaacga tcttcttcac ccattcgatt gtcttttcat ctgggttttc tgctacacgt
    59941 ccatcagcga ttagataagg cagacacatg tcagtaaatg catctagatc cattgctttg
    60001 atgtaatggt tgtttaccca ttgcagtttt ttcgcatcga aagctgcggg tgatttactt
    60061 aggcgttttg gatcgaacat tttgatcaat tcttggtgtt cgaagatttc gtcttcgccc
    60121 acaggtgacc agcccaataa tgcaataaag ttgaacattg catctggtag atagcctaat
    60181 tcgcgatatt gttcgataaa ttgcaagatt gattcgtcac gtttgcttag ttttttacct
    60241 gtttcactgt tgatgatcaa tgtcatatgt ccgaaggttg gtggtgtcca gccaaatgct
    60301 tcatagatca tcagttgttt tggtgtattg gcaatatggt catctccacg taaaacgtga
    60361 gtgattttca tcaaatgatc atcgaccgca acagcaaaat tataagttgg cataccatcg
    60421 cgtttttgga tgacaaagtc gccaccgata ttatcggatt cgaacgaaat agcgcctttc
    60481 accatatctg taaatgcgta ttctgtattg cgaggcacac ggaaacgtac aacagattct
    60541 agaccttgtg cttctttttc tgcttgttct tcaggtgtca agttcgcaca tgttcctgcg
    60601 taatgaggca tttctcctct tgcgcgttgt gcttcccgtt ctgcttctaa ttcttcttct
    60661 gtgcaatagc atttgtatgc tcgattgcta gcaagcagct gatcaatcag cggttgatag
    60721 atttctttac gttcagattg acggtaaggg ccgtattctc caggattttc aggagattca
    60781 tcccaattca ttcccaacca agcaaggttt tccaactggc ttttttcgcc atcttcgata
    60841 ttacgttttt ggtcagtatc ctcaatacga ataataaagt ctccatcatt gtggcgggca
    60901 aacaaataat tgaataatgc agttcttgca tttccaatat gcaggtgccc agttggactt
    60961 ggtgcatagc gtacgcgtac tttcgtcatt ttcttcctct tctttcaaac tagactttga
    61021 ctaacagcaa aaattccgtc agttacggct tctcaaatat catttattta aagccactca
    61081 gcacaaattt taccttttct aaggtaaata gtaaagccaa ctggttgaat ttccttacca
    61141 gttggctgaa tttctttttt attttgattg tttagtcttt ttctctgtct cttttttatt
    61201 gtcgattcct cgtcctgcat gtgcaggttt agcaaagatc atgcgtccag cagctgtttg
    61261 aagggcactt gttacaacga cttcgatacg ttcattcatg taatgctgtc catcttctac
    61321 gacaaccatc gtgccgtcat caaggtaggc tactccttgt tggcgttctg tcccggcttt
    61381 tactactaga acattcatcg tttcaccagg gatgacgact ggttttacgg catttgccaa
    61441 cgcattgata ttaagaacag gtacattttg gaattcagag actttattca agttatagtc
    61501 attggttaca atgacaccat ctaaaagctt agccaatttg atcaatttgc tgtctacttc
    61561 actgatatct tcaaagtctc catcgtacat ttcgacagat ataccctctt ctttttgcaa
    61621 ggcatttagg atatctaatc cgcgacgacc acgcacacgt ttcaaactat cccctgagtc
    61681 cgcgatgtat tgtagttcgt aaagaacaaa attagggatc atcaaggttc cttctaagaa
    61741 tccagtcttt gtaatatcat aaatccgacc gtcaatgatc acgcttgtat ccaagatttt
    61801 atatttatgg aaattttctc ctgattttcg ttctaacagt tgactctcaa cttgactagc
    61861 tgtttcttgc tcgatttttt tgcttcgtga accaaaaact ttcttccatt cttcgattcg
    61921 tgtcgttccc attctcaatc ccagatagcc taagatgatc atcagtaaaa tcggcaatac
    61981 actattgacg aatggaattt ccatattgta aagaggggtc gagatcacaa cacctaaagt
    62041 caatccgatg atcgcaccga tcgcaccgaa gagaagataa gtcaagctga cctcacttag
    62101 tttttgttct acttgtttga taatagctga gatatgtttg gccaacagca aagataataa
    62161 aaagaaaata agtgcgccta acaggctgtt ggtaaatgta ttattcagcc atccgtttga
    62221 ctgttgaccg attgcctgcc aagctgaggg taaaaatgaa atacccaagc ttgcgccgac
    62281 aatgatcatc agcaaagtaa tgacacgttt ttgcatcgtt tttcctccgt ttcgacaatc
    62341 atttattagt tagcggaaca cacgcttcaa cgtttcagcc aaagttgcta ctccaactat
    62401 ctcgattcct tttggcgctt cccagttacc taagttgttt ttaggtaggt atacttttgt
    62461 gaagccgagt ttttggactt ctttgactcg ttgttcaata tgactgacac gtctgatttc
    62521 tccagtcagt ccaatttcac cgatgaaaca ttccgttggc tgtgttcctt tttctttata
    62581 gcttgaagca atactgacag cgatagctaa atcaatcgct ggttcattta attttacccc
    62641 acctgcagct tttaagtaag catcttgatt ctgaagaagc aacccagctc ttttttccag
    62701 cactgccatg atcagagata cccgattgaa atcaagacct gttgttgttc gtttggcatt
    62761 tccgaacata gtaggtgtga ctaatgcttg gatctctacc aaaattggac gtgtcccttc
    62821 cattgccaca acgatagcag agccagttgc accgtctagg cgttcttcta agaaaatctg
    62881 tgaaggatta gcgacttctt ccaatccatg ctcatgcatt tcaaaaatcc caatctcatt
    62941 cgttgagcca aatcggtttt ttactgctcg caaaatccga aaactatgat gttgttcacc
    63001 ttcaaaatat aacaccgtat ccaccatatg ttctaacatt cttgggccgg cgatcgagcc
    63061 ttctttggta acatgaccga cgataaaaat ggctatccca tttgttttag cggttttgag
    63121 tagttctgca gtggtttctc ttacttggct gacacttcct gccacacttg tgatgtctgg
    63181 ctgcgtcatc gtttggatgg aatcaatgat aacataatca ggggagatat gttcgatggc
    63241 acgactgatt tcgttcatat ctgtttcagc gtatagataa aattctgtgt tgatacttcc
    63301 gaggcgttcc gcgcgtaatt ttatctgctc tgcgctttct tcgccggata catataagac
    63361 ttttccgcct atggctgcta attgctgaga aacttgtaac agcaaagtgg atttcccgat
    63421 acctggatcg ccaccaatca agaccataga ccctggcacc acacctccgc ctaacacacg
    63481 gttcagttct tctaattttg ttttgatccg aggttctttt ttagggacga cgtctgcgat
    63541 ttttgttggt tttgcttttt ctcctgtcaa acttgtccga gttctgcggt ccgttgtatc
    63601 ttgttcgatt tcttcgacca tcgtattcca ttttccgcaa tttgggcagc gtcccaagaa
    63661 cttaggcgac acatacccgc aattttggca aacaaattgt acttttgatt tttttgccat
    63721 tgtaccgcct ccttttatcc taatcatgag acattaaatc aagcttaagt atctttaacg
    63781 ttttatttac tggaagaacc aaatccgcct tgacgttgag cagtcgtttg atcttgatct
    63841 gcttttaaaa acggcaaaaa gatcccttgg ccgatacgct cgcctttttt gatcactaca
    63901 tccttgaaac caaagttggt gaattggaac atgatatggc cttcatttgc ctcattattg
    63961 taataatcac tgtcaatcac acctactccg tttgtcaaca ctaaaaaccg cttcaacgga
    64021 ttgcttgaac ggttagcaag ctgcaaaaat tcatcttctc ccatgtaagc tttgattcca
    64081 gtaggcacga gggttggttt caattgtttt tcattttctg atacatcttt tttcaatcca
    64141 tctgcgactg tttccaaccc ctttttccag cctgctttcc agatacttgg aatcacgacc
    64201 tcttctgctg cttcaaaatc atagccagca gcatgatgtg tagcgcgctg cggtagattg
    64261 atattttgat ctttatattt agaaataact tcaaatcctc tttttctcac cggttttcct
    64321 ccctaaataa actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa
    64381 ttcttcaaga aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac
    64441 tgcaagttgt acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata
    64501 gaacgaaatt aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat
    64561 gcaaaacgaa ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc
    64621 aatcttgcgg caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag
    64681 gaagtgatct tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca
    64741 gaacgtccga ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg
    64801 atgaatatct ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg
    64861 acaagagacg tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag
    64921 cttttgatta tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa
    64981 ttagatcatc tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt
    65041 gtcgcggcag accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg
    65101 actacaaatc ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg
    65161 gcacaagcag gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa
    65221 gccgcttcta ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg
    65281 gcaattattt ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg
    65341 aaatgactga cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac
    65401 ttatgcctac aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga
    65461 atcaacaagc gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata
    65521 tcagtgaaac gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta
    65581 cagagatcga aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga
    65641 cgaacaccga cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg atcggcatca
    65701 tttacgaatc tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga
    65761 atgccgttat ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat
    65821 tattgcagca agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg
    65881 atacttctca tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga
    65941 ttcctcgagg cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca
    66001 ttgaaacagg tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg
    66061 cagtgaacat catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa
    66121 cattgctgat ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg
    66181 tagaacacgg ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg
    66241 ctttagcaga agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag
    66301 tggtcgattc tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag
    66361 aaacgatcgt gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg
    66421 cggctgtcta tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg
    66481 cagagattgg tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat
    66541 taacttccac aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt
    66601 gaaacattat tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg
    66661 tcaatatgtt tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg
    66721 cttttctggg agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga
    66781 gtcaacggga aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac
    66841 gcttgcacat tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt
    66901 ttttcgatca ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat
    66961 cttataaata tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg
    67021 tacgataagt agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg
    67081 acaaagattt tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg
    67141 gtaaatctgt ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat
    67201 aacgaagcag ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt
    67261 cagccagtag ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt
    67321 ttctagtttc accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca
    67381 accgatcatc tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat
    67441 attcttccag catctattat ccccccattc tttttcttaa atgaatttta gcttacctac
    67501 tgaaaatttt ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca
    67561 catccccttt ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat
    67621 attcaacaaa catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga
    67681 cagtctcaca acctcttggt aaacggcgtt caatcagctg accctcggta ttagctcaaa
    67741 aaacggtaaa atatgagaag ctatttttcc gttttgtttc tctaatactt aggataaaac
    67801 gctgatttta caacctctgt taaacggtgt tcaatcagct gtccttcggt attagctcaa
    67861 aaaacggcaa aatatgagga actatttttc cgtttgtttc tctaatactc aggacaaaac
    67921 gctgatttta caacctcctg ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc
    67981 caaccaaacg aaaaatatga gaagctattt ttgtttggct gttcttatga cttgatgcag
    68041 gacagctttt tcacaacctc tgatttctta ttcttcttca tttgtagcaa aataattacg
    68101 gtgataaatg atacaacata cactgataag aacaatggca cctgcaaaat aaaacgcata
    68161 gctttcacca atatgttcac caatcacacc tgcaataaat ggtgctaaag ctccaccgaa
    68221 aaagcggaca aaactatagc tggacgaagc aacgtttcgt tgcagacctg gtatttccat
    68281 agcaatcgtc gtcaacagtg tattgataat tccttggaaa aatcctgcta acacaatgct
    68341 gatcggtaca aggactggca tagaagttcc agctcctaat aacagcaaac aaataatgaa
    68401 taagacaaat gctgctaaaa ttgtccgata agtcgtaaac ttccgctcaa gagccggtgc
    68461 aacgaaaatc gaagcaatag ccagaagcac accccagcca aaaaatacaa aaccgacttc
    68521 taattcagaa tagccaatca ataaaaatgg cgcatacgca agtaaagtaa agaatccgaa
    68581 attatataac aatgcgatta gtccaactga acgaagttta tgattgctta acgctttgac
    68641 accttcccaa aggcctactt ttctctttgg tttctcagtt tcaggtaaaa gtaccgctag
    68701 ggctataaaa ccgcaaaaca tcaaaaatgc taccccaaaa aatggataac gccatgaacc
    68761 actgcccaag accccaccga ctagagggcc aacagccatt ccgcagccaa gtgctgcttc
    68821 atacatcatg attgcttttt ctgtgttacc aactaaaata ctgacgatcg ttgctaaagc
    68881 agtagagaca aataaagcat tcccgacacc ccagccggca cgtaacccaa tcagcattcc
    68941 gatattccca gataatccgc caagaccagc gaatacaacg atgataaaca atcctgtcat
    69001 caatgttttt ttcaaaccga tccgactaga taagaaacct gtaaatagca tcaccacacc
    69061 agtgactaac atgtaactag taaataacag ggtcgtttca gctggcgtag cgtttaactg
    69121 tgcagcaatc gtttttaaga ttgggtctac tagacccact cccataaaag cgatcataca
    69181 ggtagctgct acaactagcg ctgaactttt cccctttgtt tcttcatttg tttgttcttg
    69241 tatatctatt tgattctcca atatttccat ccctttctct ctattagcat catacatgat
    69301 ttattttaca tgtcaatatg cacatgtaaa acgtggaaac tttactgtga tataataaag
    69361 aaaataagac gagaggtagg atttttatga atactttagc ctatatatta ttatgcgtgc
    69421 ttttatccaa accacgttct gggtatgaat tgaagcagct gatcacaatt ttttgggaag
    69481 cccatcacag tcagatctat acaacgcttg ccaagttaga ccagcagggt tatgtgaccg
    69541 ttttggacaa agacgagcac tctcaaaaaa agatctacca cttgactgat gaaggacgtg
    69601 ctcttgtaga ggaatggatc aaagaggaaa ctcccgctcc tacgcaaaaa gatgaatttc
    69661 tagcaaaaat ctatgctttt tcgacgctcg ataaaacaac agctactgga ctgctattca
    69721 ctagagaaca acagctgaac aaaatcatgc aaaaaaatca aaaaaaattg aatgacctct
    69781 cgctagacaa gaaacaagaa tttggacgtt atgttgtgat ccagcgcaga attatgcttt
    69841 gtcagcagga actccaatgg tgtcagcaag taagagaaca aatgcagcag ttttttgagt
    69901 aagggataaa cagaaccttt ctagaaatat ctattgaaga gcaaattatc ttcaataaaa
    69961 gagcaataca gttggaaaag aaaaagcgat ccgaacgaca ggccaaaacc tatcgttcgg
    70021 atcactattt ttctagaact gcattcaaga gactatgaca aaaagcatgc caaggccttt
    70081 tttcaaataa cagtgttcca tcattcatta ctttgaattg cttttagcac acttcttgaa
    70141 agcgggccaa cgatgatcaa ctgcaacggt aaagcggcaa tcacattcat tttccaagca
    70201 ttcatataag cagagaataa ttgatcaggt attccttgtt ccatgatcac accgaataaa
    70261 gacatacatg tcaccattcc taaaaccatc aagcatgaaa tagttaagat catttggacc
    70321 ttgctttctg gatttactgg tagtttaaag gcaatttttt tcgccagcac accgatgatg
    70381 aagacatcca aaatgaaagc caccacgaat ccaggcacta gaccaatcaa caaacttgat
    70441 aaatgaagat cttgatgcag ccataaatta tacgcgctca tccccaagac catcaaaaaa
    70501 cacatcattg ttgtaaaaat tattccttct tttttatttg taggcaatct gtaccactcc
    70561 tttaaatttg tcacgaaaat catcgtagca catttaaaaa tttctcgtaa gtactttttt
    70621 tattttctgc ccttttattt cataaacttc atcagccgta cgctcgacaa agtaagaatc
    70681 atgagaagta aacaaaatcg ttcccggata tatttgcatt agcttctcta aagcttcgat
    70741 cgttgccata tcaataaaat tagtcggttc atctaacagc aacacattac ttggcttagt
    70801 aaaaagcaaa gcaatcgtca gacgggtcgc ttctccccca cttaatgtac ataacggttt
    70861 cgtcaaagtt tcctgagcaa accctaagtg attcagcaaa ctacggacaa gcgattctga
    70921 aaactctgtt tgtctcatga caaaacgaat gatggattct tcagatgtca tttgataacc
    70981 cttttgctga tatacttgaa agcttacttt cggagagaga aggattcctt ctccttgttt
    71041 gcggatatgt tctaataagg ttgtcttacc tgaaccattt tcaccgacaa tcgcgatttt
    71101 tttacctaaa gaaaactgaa aatcaccatt taccagtaat gttctcccac ttctttccaa
    71161 ttggacattt tgtcccatga tgggatagcg gctgtggagt tcaagagatt ttggtactgg
    71221 aaaacggatt tgtttcatct gctgcggctt ggttgtttct cctatttttt ggagtcttct
    71281 ctctaatgtt ttcgcattct tttggatggc tttttgtaca ctgtcttttt gtttagagga
    71341 agacaaccga tccagtctga tttgttgctt tttcttttta ctggacactt tttccaactt
    71401 ttccgcttgt tttttctttt cttggatcga tttttccaat cgttttttct ctttcatgat
    71461 cgactctgct tctcgtagct gagtcttttt ctccaattcc ttttgagtga gataggcact
    71521 ataattcccc gaaaactctc ggatttctcc gtcttttact tcccagattt tcgatgctag
    71581 ctcatctaga aaatagcgat catgactaac aaaaaccagc gtaccataat aataacgaag
    71641 ctcttcaatc agtcgatcaa tactttttct atctagatgg gttgttggct cgtctagaag
    71701 catcccttgt tcataagaag aaagaagacg tatcaactcg atctttcgtt cttcccctcc
    71761 gctccatttc cgctctgaag tttcttccag tcttaactgg aaatgactgg ctaattccaa
    71821 atttaggtcc tttgcttcag cgagataggt taattgaggc aggtagttga aggtgatctc
    71881 tgtttggatt ttcccatgat cgggaaaaag ttcaccggct attagtttga gcaaagtcga
    71941 tttaccgaca ccattttctc ctatgatccc tatacgatct tgctgataga cacgaagaga
    72001 atcaatagat agttctagct ggttgccaaa gctgacttgt agatttgtta tatttactgc
    72061 taaattttcc atatgtttta cactcctatt tcatgtaaaa caacaattat cgatactaaa
    72121 aaagagcaga aaaatctgtt tgcaaaaaaa ctgcatcaca tcttttccta ctctgaacga
    72181 aataactatg actaaatcac caaatctgtc atagctttca cctattctac tgtcgttcga
    72241 aaaaatggac atactccatc catgaaattg ttgtttatac atgtcttttg aatttattta
    72301 gttgttattc aaaagttcga aacgtaattt catcgatgca gtcatttttc cgacctcctt
    72361 ttttattagg ttaccacaca cgattcaaaa aaagcaatag ctactgaata gtaactgaaa
    72421 accttttatt cgaacatagt gatcttttct ggtgctaaac gaacagactc gtaaccttct
    72481 tctaccgctt ttcctattga taaaataata actggaacat aacggtcttt atccaaacca
    72541 aatgcctctg ccaactgatc tgcttcgaat ccaccaattg gatttgtttc atagccatga
    72601 tcacgcgcaa ctaacatgaa ttgcatagcc gctaagctag aatcgacttt gactacatcg
    72661 ttcatttctt ctcttgaaaa actcttgtaa taaggaatga tcgcagctaa ttgttgatct
    72721 ctgacttctt ttggcatctt ccctgattcg taagcttggt tgtagatttc ttcgccatat
    72781 tcgtaacatt gcatatcgcc aaaaatcatg accattgcag aagaagtatc attttgtctc
    72841 gtattgaaac ggatcaaagg acgcaagatt tcttttgctt cctcgctttc agcgaccaca
    72901 aagcgccatg gtttcatatt gacagaagaa ggtgcagtcg tcgcttcttt gatcatttcc
    72961 agcatttctt cgtgtgggat tttgtatgtt tcatcatata cgcgaacgga tcttctacca
    73021 gtcataattg ttgaaaaatc atttttttgt tccattatta ttaacctctt tcgtttctgt
    73081 tgttatattg caaagttggg tattcaattt atctaaaagt gttagaaatt gtttcatttc
    73141 tacatcgctg aaagccgggg atagcacgga acctgtatct ttatggtcca tttcacaatg
    73201 cttgattttc ttctgggctt tttctgttag cgctacatac acttcccggt tgttctgagg
    73261 atttctctga cgcgaaacat agccatttgc ttccaatacc ttcagatgac gagtaactgc
    73321 tccttgatca atctgcaact tttcttgcaa actgctttgt agcaatggat tttcttcttt
    73381 cagtctcatc atgatctcgt atctagtaag acttataccg atattttctt caaagatctg
    73441 gacgattttt tgatcggcta atttgatttg atataaaagg tgactaatct cttctaaaga
    73501 cataatttcc tcctttctaa taattgactc gtcaacgatt gacttgtcaa taatagaata
    73561 aaaaaagaaa aaagtcaaag aatcagctgt gtaataaaaa aaacgctctc aatagaaaag
    73621 tatagagaaa agaaattttt tgtagaatga acggaaaaaa tgataaagaa agcacaacgc
    73681 taaaaccaag attattgatt cttatcaaac tatagaagat agctcttctg cagtagataa
    73741 ttttacaccg ccaaaaattt ccgaaatcgc cttttgcaag aatcccgtcg aatattagaa
    73801 attggatcag tatgcttaaa cagtaataaa aaagttatga caagatgttg tcacaacctt
    73861 ttttgagtaa acggtgttca aaaacttgca tctgcggtaa taagttcaac caaacaaaaa
    73921 acatgaaaat ctgtttttgt ttggctgttc ttactacttg atgcagggca gctttttcac
    73981 tacctctttt taataaccat aaaatcctat tccttcatat ctccagccca tcggtgttag
    74041 ccaatctctt tccttagcac tcaaggtata gtgatgggta ccagtttttt catttggatt
    74101 aaatagtcga taaattggct tggaaccacc agaataaaaa cttagacctt cataacgcca
    74161 accgattttt gttaaagagt tgatctcaga ttgtgccatt gtgtaatgat ggtctccact
    74221 gtaaggattg tataaacggt agacaggttg tccgctgttt ggtgcttgcc aagcaatccc
    74281 ttcatattcc cagcctacag ttggcagata atttttttct ccggcattca atgtatataa
    74341 atgttctcct gtatgacggt tatacagacg atacatcgga gaagttgaag ttggtgctgt
    74401 atcatactgt gtcagattat aggtttcgat tacattgttc aatgtagaac cataattagg
    74461 cgcagtggca taacgacctg ttagccaagc cgtagcatct ttgtaagagt tcgtattgct
    74521 tttccatgca ccggaatagt agtaaacgcc tggctgaaaa gaagtcgtct tcaataccgt
    74581 tacgttatct tgcaatgatt ctttgtacga aggatatttt ctaaaagcgg cattcaccgt
    74641 aatccattgc ccattcacat attccgaagt aggcatcgta acagattggc cattatagct
    74701 tcctttaatc ccaaatagat tgtaattagg ggcagaagcc aaagtactgt tcccccagcc
    74761 actttcaacg atggcttgag caatcatgac tgatgcatat aagtcattgg cacttgccaa
    74821 actttgtgca gttgaggcaa tactatcaat gaacgcttgt tgagaattag cagatgagcg
    74881 agcataaaaa gcatagccag tatagttttc gaattcagac aaatctgctt gatgggtatg
    74941 tgtcaatgtt tgcccatctg tcaattggtt cgtgatttct tctgcattct ctggattgtt
    75001 ctgagcttct gctgatgtac tgtcttcggt ttgtgtttgt tctgttgttt cagttgttgg
    75061 ttcttcggtt gaaattgtat tttctgagac agtagctgac tgcaaagtac tttctgtatc
    75121 gttttcaatt cctgtttctg aagaactcga tgtctgagtt gcgacagttt ctgtcgattc
    75181 ttcagctagc actgttgtgt tggcaactgc tagatttcct aaaagaataa cgctaaataa
    75241 taaattcttt gctttcatct tttttctcct ttgaattatg aaacttttcc taaataacag
    75301 tgtaactaaa aaagtaaaag caagtcaatg tccatattta aataacattt gtttcttttt
    75361 aataaatttg ctttttctgc ataaaaagac gtaaatgaag aatattcttc tccattcacg
    75421 ccttaaattg atggtcgttt gaataactat ttaacaagta attctctctg gatattatcc
    75481 gttatggatt atacttcctg tgtcgtcgga cggataatca tgttgctgac agacatccgg
    75541 tctggcgttt ctatcgcaaa tgctacagca gaagctatat cttcagatgt taatccccat
    75601 tctttttgcg cttcatgaag ttcaagagca gcctttttgt cgctgatggt ttgatataat
    75661 tcagtttgaa cagctcctgg agagatgatc gtagatttga tgttattttg ccgttgctct
    75721 tgacgtaatc cttccatgat tgcacggaca gcaaattttg ttccgcaata aacggcggaa
    75781 tcaggataaa caacatgtcc cgccacagaa tcagttgaaa tgatatgccc agatttttgt
    75841 ttttccatga ttggaagtac ggcggaaatt ccatttaaca cacccatgat gttgatatca
    75901 agcatatttt gccattcatc acggtgacct tcaactaatg gagcggttgg catgattcct
    75961 gcattattgt acaatacatc gattcgaccg aacttttcca tcgctaaatc gataactgct
    76021 tggacttgtg caaaatctct tacatctgct gtttgaatat atagctcagc ttctggtaaa
    76081 gattctttta ttgcttttag gcgatcttct cggcgtgcag ctataactag tttagcccct
    76141 ttttctgcta atagcttcgt tgttgcttca ccgattccgc ttgaagcgcc catgatgaca
    76201 atcacttttt ctgataaatc attcattcta ttctcctcct gttttgaaaa atctcttgct
    76261 tatactcact atctcttatt tcatcttact gttcaaataa tccgttcgac aggtttgacg
    76321 attttggcag ggattcctgc aacgatggta ttgtctggga catctttcgt aacagttgca
    76381 ttcgctgcaa caatggcatt ctcgccaatc gttacgcctg gcagaatagt tgctcctgct
    76441 ccgatccaag catttttctt gatgtggatt ggctttactg acaagcctct tcgttctttt
    76501 ggatcggtta gatggttcac cgtgatgagg ttgacacgag gaccaatcaa tactgaatct
    76561 tctaaacaga tgccacctaa gtcaacgaac atcacttgac gattgataaa gacatttttc
    76621 cctatactaa tatgtttgcc gaaatctgta taaaaaggca gcagaacagt caccgtatca
    76681 tctactttgt cactaatgat attttctagc cgtttgcgga tatctgctgg ttcatggtac
    76741 ccagtattca gcgctgctaa aggctcttcg ttttccgctt ggatctgatg gatcacttca
    76801 aagagcggat ccgttactcg aatctcttta ttgacgattt gtttttcgat gaggcttgtc
    76861 attttgctcc ctccttttat ccttacttta taacggacgg cttatgatgt ctaatactta
    76921 taaaacatac ttaactattc cttttaacta tacttagtgg tatgcttttt taaaaacagg
    76981 agggaaaaaa tggatattcg tatactgacg tattttttag cagtagctag agaaaaaaat
    77041 atcagccgag cagcaaaatc tctgcatatc acgcaaccta ctctctcaaa acaactgaaa
    77101 gatttagaag aagaacttgg tgtgacactt ttcactcgag gctcccgaga gatacagttg
    77161 acggattctg gtcgatatct ttattcaaaa ggaaaagaaa tcttgtcgtt ggtagaaaaa
    77221 acggcgaaca atttaacaaa tgacgcaatc atctctggag aaatctacat tggcggaggc
    77281 gaaactaaag caatctcttt catttctgat agccttcatt cgttgatcaa ggcgcatcct
    77341 gatatccagt ttcatttata tagcgggaat gcagatgata tcatagacaa actggatagc
    77401 ggattacttg attttggact cgtaatcgaa ccgacaaaca aacaaaaata ccagtcattt
    77461 cgactgccag cccaagatcg ttggggactt ttgatacatc ggaatcatcc tttagcaaag
    77521 caaacagtca tccatccgca ggaactgaaa actgttcctt tgatcatttc ccggcaaacg
    77581 tcagtggaca gtcaactagc tgaatggcta ggtttcagca tagatcagtt atcgatcgtc
    77641 ggaacatata acttattgta caatgcttct ttgatggtcg ataacgggac ttttggtgca
    77701 ttagctctag atgggatcat caatacttat gggacggaaa aagtattcat cccactcgat
    77761 ccgccattac aagcttcact gaatctcatt tggaaaaaag accagcctct ttctcctgct
    77821 gctcgtaaat ttttagatgt cctaacggag aaatacagtt aagtagagtt tttttccttc
    77881 gctttcagct ttttaacaac gaataagtta agcaaaagat ccaatgatta aataacataa
    77941 gtttcttaat ttgttccttc cttttatttc tgataattcc agtaaattac tataattata
    78001 aaaaaagaat tgacaaaaca taactgtcta tataaaattg attacgttaa caataattcg
    78061 tttcatgtga ctagatcata ctaggcatga aaaatctctg aggaacatgg tttccctagg
    78121 aggtttttcg tgtctatttt tgtttggccg caatcaactg gtctagcaaa cgtgaaaacc
    78181 aaatcttcaa aaagggtgcg cgcatgaaaa tcaaaaagat ctttaatcaa aatgcagtat
    78241 tggtagatga cggtggacag gaaaaagtag ctattggaaa agggattgga tttgacagaa
    78301 aaagaaatga tttgattttt gatcgtgatg tcgaacggat atttgtcatg gaaccagaag
    78361 gtcaaatcaa gttgcaaaat ttacttaatc agattgatga acgcttttta tttgcagcag
    78421 aaacgatcat tgatcatgcg gaaactgttt tgatggaaaa gttgaatgaa catgtattga
    78481 tcgctttgac cgaccacatt gctttttctg cggagaacat taataacggt atcgtcatcc
    78541 gcaacaaact tttaagagaa atcgaagtgc tttacagtga tgaattcagt attgctcaat
    78601 gggcagtaga gtatctgaca aaagagcttg ggattccata tacttatgac gaagcaggat
    78661 atatcgcaat ccatattcac agcggacgaa gcggtcggaa caataaccat cgcagtatcc
    78721 gtgaagtgac gattgtatct gacatcatcc atttagtgga atcagaattg gaaacagata
    78781 tccattcaga accattttca ctgaattatt caagactggt gaatcattta cgactgcttc
    78841 tacaacgaac ccatgcgcaa caatatgctg ttttagacac ggaaatcgta gaaatggtca
    78901 aacgaaaata cccagaaagc tataagattt ctaaaaaaat ccgtgtgttg ctgaccaaag
    78961 aataccagct cgcgatcacg aaagaagaac tgggttactt ggccatccat atcgagcgtt
    79021 taagaagcgc aatcgtacaa acaaatgtta acaatcatga ggaggaaaaa aattgaaagc
    79081 atatctacaa cgaatgggac gttcccttca acttccagtt gcggtcttac ctgctgctgc
    79141 tttactagta gggatcggaa actggtgggc atcttatagt aacgacattg tcgctcattt
    79201 tttacaagct ggcggtaacg cagttttagg tcaattacct ttgctgtttg ccgttgggtt
    79261 agcactaggg atgtcagtgg acaaatcagg tgctgctgcc ttggcaggtt tggtcgcatt
    79321 tgaagttcca gtaaatgttt taaaaactga ttcagtagct actttattga atatcaaacc
    79381 agaatcagtt gatccatctt ttgctcagat cagtaacgta tttattggta ttattgccgg
    79441 attgattgcg gcagctcttt ataatcgttt ccacgaagca aaattaccga tggctttatc
    79501 tttctttagc ggcaagcgtc tagtgccaat cttggctgcc tttgtcatgt tgctgatcag
    79561 tgcagtgcta ctgctcgtat ggcctccggt ttactctgct ttagtttctt ttgctaaatt
    79621 cattttgagt ttaggctggg gtggtgcagg tctttatggc ttcttcaacc gtttactgat
    79681 ccctacagga ttgcatcaag caatgaactc cgtgttctgg tttgacgtag caggaatcaa
    79741 tgatattggt aaatttttgg cttctgaagg aacaaaaggc gtaacaggaa tgtatcaagc
    79801 tggatttttc ccaatcatga tgttcgggct tccagcagga gcatatgcta tttacagaaa
    79861 tgcacgtcca gaaaacaaag caaaaacagc ttccttgatg ttagcagcag cttttgcttc
    79921 attctttact ggtgtaacag aaccacttga attctcattt atgttcgttg cttggccatt
    79981 atatgtcttg catgcaattt tcactgggat ttctttagca gtgagtgcat tcttccattg
    80041 gacagcagga tttgccttta gtgccggatt tgtcgattac ttcctatctt tgaaaaatcc
    80101 gattgcgaat cagccattga tgttgatcgt ccaaggatta gtaacagcag tgatttacta
    80161 ctttggcttt aactttgcta tcaaaaaatt ccatctgatg actcctggcc gcgaggaagc
    80221 agatctttca gatgacgata ctgcaacgac taattcagac aataaatatg cggcacaagc
    80281 aagcaaaatc tacgctgcat taggcggagc cgacaatgta acttcgatcg ataactgtac
    80341 tacacgcctt cgtttgcaag tcaaagatac aggcctgatc gatcaaaata aaatcaaagc
    80401 cactggcgta cctggtatga agatcatcga cggaaaaaat gcgcaagtca tcgtaggtac
    80461 agaagtacaa ttcgttgcag atgagatggc aaaactacat ggtggtgctg caacacgacc
    80521 tgcacaaaca aacaccgtgg ttaaaacaga aacatttgcc ggtggaacga tcgaacaaga
    80581 tatttacgct atcgcaaatg ggaaactgat tccaatcact gaagtttcag atgatgtatt
    80641 cgcggaaaaa atgatgggtg acggctatgc tgtattacca gaaaatggag aaatcttctc
    80701 tccaatcgct ggtacgatca caaacatttt cccaacgaaa catgctgtcg gaatccaaac
    80761 agatgctggt atcgaagtct tgcttcatat gggcatcaac actgttgact taaaaggcga
    80821 accattcact ttatatgtaa aagaaggtca aaaagtcgca agaggtcaat tgattgcttt
    80881 agttgatctt gccgctattc aatctgcagg caaaaacacg gatatggtcg ttgtattcac
    80941 aaatggcgac aaagtccaaa gcttagaaat cgaacccgca cgtgatgtaa aagcaaatga
    81001 taaaatcggc tctgtttcca acaaagcctg agccatcgtc ttccccagac ttttttatcg
    81061 ataaacaaac aggagctgag acaaaaatct tgtctcagct cctttttccg aataaaaacg
    81121 gcgttctcct gccaactccg cggtaataag tccagccaaa cgtaacatat gaagagctat
    81181 tttttatttg actcttctta ttacttgatg cagaacagct tttttacaac ctctgataaa
    81241 cggtgttcaa aaaccagttt atcggtatga agtcaaattt atgcaaaaat gtggaaagcc
    81301 attttcacaa aatttttctt catactcaaa actaatcggc tttttcacaa cctctttata
    81361 cattattcct gatccaatat ttcttttaat gctgcttcaa gagaagttaa tggttttccc
    81421 aatacttttt ctaaatcatc agaggaaata gctaactgat cctttttaat atcatgttgg
    81481 atcgctaaaa acatgtcagc tgcttcttga gggaatccgg cttcttttag attttcgaca
    81541 aacgctgcat cacttgcttc aataatttct aactttttac ctgtcgcacg ctctaatgcc
    81601 ttggctaatt cttcatagtc agttagattg ccggataatt ctaagatttc tggagagtca
    81661 ctttctacta gtacttttgc tgcagcttct gcatattcac gtttcaatgc ccaacctgtt
    81721 tttccatttc ctgcagcata aacgaattga ccagttttta aagcctgccc aatgataggt
    81781 aattcattct ctacatacca attattccgt aaaaatgtat gggaaatacc tgaatcctta
    81841 atcatttttt cagtgaattg gtgatctggc gctaatacac tgatcgagtg atcggcatct
    81901 gcaaaacttg tataagcgat ataagatacc ccagctgctt gtgctgcctt cactacattc
    81961 ttatgttcgg cttgacgatt tcctggaacg ccagagacaa aaagcaatcg atctattcct
    82021 ttcaacgctt ctttcattga gtctaaatca tcataatcac caatacgtat attaaatcca
    82081 gcttctttca gtgcttctcc tttttcttca ctacgtacta gtacatagat ttcattttgt
    82141 ggaaccatct tttttaagaa ttccagcgca taaccaccaa aacctccagt tgctcctgtt
    82201 actaaataat gcatatttgc cactccctat tctttttatt tgttaagatt tacagctgta
    82261 catttataaa ttacaccttt atcataaaga gtgtggtaag ataagtcaag caaaaaaaat
    82321 gaaaggatga caactgatga agtattctct gcaatttagt gatgcaatcc atgtacttgc
    82381 ctatattgaa atgtttcaag atacccgatc actttctagt gagatgattg ccagtagtat
    82441 cgaaacaaat gcagcaaatg tacgtaaaat catgagtcag ctcaaaaaat ccggtttgat
    82501 ctttacaaaa accggaaaac cgcagccttc ccttgccaaa agtccagatc agatttcttt
    82561 actagatgtc tatcgcagca tcgaaggtaa tacacagctt atccaagtcg atccaaaaac
    82621 aaatccaaat tgcgtcgttg gtgcgaatat ccaagaagct ttagaagaaa gctacgctcg
    82681 gctgcagaaa aaggcagaag aagaaatggc taagatcaca cttgatagtc tgatacataa
    82741 aatagccgtt ttggaaaaag aaaaaagacc agaaaacaca ggcctcgtag agagattttt
    82801 ataattctct ctactgtgcc tgcatttttc tgatcggaat cgtctacata aattctttta
    82861 ttctccgggg gacaaaagcc ttgtcatgcc cctttttcct ctctaatgct caggtcaaag
    82921 cgctgaagtc atgacgtctt gataaacggt gttcaagtag ctgaccctcg gcattagttc
    82981 tgaaaaatgc aaaatatgga aagctatttc ccatttttcc tctctaatgc tcaggtcaaa
    83041 gcgctacttt cacgacctct atattatcat ggtttattga ctaccggtat ccagatttct
    83101 cccatatatt tcccgttggt aactcccatt tcgactacag catttggacc cccaacatag
    83161 gcgtaatctt ctacttctct taaaacatgc ccaaaggccg tattcgtcaa atcatttctt
    83221 aattcttcag gcgattctgc taaagcgatt acaacaagat acgttccttt tgggaatggg
    83281 atgattcggg tagtgtttgg aacatcctga ctcgtttgaa caccgacata gtacatcatt
    83341 ttcccgttat agggttcgtt gactgtaaag aaataatgat tttcagcagc ttgtttcaat
    83401 gcatctattg ttccattacc gataactttt tgctcaaatg cttgtttctc tgcagccaaa
    83461 ccgacaaaat ccgtattgct actcttcaat tccacaccaa tacccgtcac cgtaaaatct
    83521 ggtttttctt cgatcctata ctttgccatt tctcccaccc tttctattat attaagttca
    83581 tcttacataa aattaaaggg aaatgacacg attacgctct tttttctagg gatattttta
    83641 ttttttaact atttttttgc tttcactgca atccaaatct cactgtaaac attactgaaa
    83701 tcctctcccc attttgtaaa agagatctcg ggagcttcga ctaactcata atctgaagaa
    83761 ggaagccatt ctgcatagat tttcccccaa gtatcttgta aagtttgggg aaatggccct
    83821 ttattaggaa aaatggccca agtgtgttct tctatagcaa gcatttctaa atcagcgtac
    83881 ggattttctt ttgtcgtagc aaagccaatc atgtgcatca aactgccctt ttcttccaag
    83941 cgggctccat caaatccata agaaacgttg attacttgat ttggatataa atctcctaac
    84001 ttatgcatct cttccctttg agtctccgtg atcgtctgcg ctaacttttg tatttcttga
    84061 ttgactcctt caaatttgac aggaactttt ttcgccacac ctgccacata aaactgttct
    84121 ttcttttcga tacgaaattc catcgatttt cctcctctga catcaataaa aaatgtcaag
    84181 cgtggaaaag atttttgtat gccgtatctg ctgatttctg aaggcagata tcctgaccaa
    84241 tcacgaaaag ctcttgaaaa accttcgact gtttcatatc catatctaaa cgccgtttct
    84301 gtcactcttg atccatctag gagatcgcga ttagccaaag ccaatctacg gtttttgata
    84361 tagtccatta gcgacatccc tgcaataaac gtaaagatgc gttttaattg atattcagag
    84421 attcctgtat tttcggctat tattttatag gaaagtcctt ctgtcaaatg ttcttcgatg
    84481 taatcgatca attggttcag atctttaagc atatttatct tcctccttgc ttgccttttt
    84541 atcatattat caaagaaagt caattacttg atgaatcagg aaccaattta gtcagatgta
    84601 gaaaatgttg tatggatcta aagaaaattc tcaagcagcg tttcgtaacg gataattggt
    84661 attatctgcc tatctatgtt ttacctttag aaaaataaat tttggggaag acacattcta
    84721 agtaaacaaa gcggtaaaaa tatcttttat tcccttagtt aatccgctta ctacctactg
    84781 tacaaagcaa attgttgttc aaactgctga tcagaacgct gcttggattt ttctttgcga
    84841 tacatcatac ctcttttatc aattggaaca attatttatt tctaacaaaa tcacttcatt
    84901 tttgtcagat aaataaa
//
LOCUS       DAYYFU010000006        84310 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000006 DAYYFU010000000
VERSION     DAYYFU010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 84310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.006"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            281..973
                     /locus_tag="ACHA34_000506"
     CDS             281..973
                     /locus_tag="ACHA34_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8791184.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1181..1471
                     /locus_tag="ACHA34_000507"
     CDS             1181..1471
                     /locus_tag="ACHA34_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791185.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1743..2092)
                     /locus_tag="ACHA34_000508"
                     /pseudo
     CDS             complement(1743..2092)
                     /locus_tag="ACHA34_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            2185..3555
                     /locus_tag="ACHA34_000509"
     CDS             2185..3555
                     /locus_tag="ACHA34_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFM8791186.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(3795..3878)
                     /locus_tag="ACHA34_000510"
     tRNA            complement(3795..3878)
                     /locus_tag="ACHA34_000510"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3842..3844),aa:Leu,seq:gag)
     gene            3951..4112
                     /locus_tag="ACHA34_000511"
     CDS             3951..4112
                     /locus_tag="ACHA34_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791187.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            4261..5478
                     /locus_tag="ACHA34_000512"
     CDS             4261..5478
                     /locus_tag="ACHA34_000512"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8791188.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            5662..6498
                     /locus_tag="ACHA34_000513"
     CDS             5662..6498
                     /locus_tag="ACHA34_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8791189.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            6574..7722
                     /gene="nagA"
                     /locus_tag="ACHA34_000514"
     CDS             6574..7722
                     /gene="nagA"
                     /locus_tag="ACHA34_000514"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFM8791190.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            7999..9000
                     /locus_tag="ACHA34_000515"
     CDS             7999..9000
                     /locus_tag="ACHA34_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFM8791191.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
     gene            9019..10038
                     /locus_tag="ACHA34_000516"
     CDS             9019..10038
                     /locus_tag="ACHA34_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFM8791192.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(10140..10856)
                     /locus_tag="ACHA34_000517"
     CDS             complement(10140..10856)
                     /locus_tag="ACHA34_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8791193.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(10825..11610)
                     /locus_tag="ACHA34_000518"
     CDS             complement(10825..11610)
                     /locus_tag="ACHA34_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8791194.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(11601..12350)
                     /locus_tag="ACHA34_000519"
     CDS             complement(11601..12350)
                     /locus_tag="ACHA34_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8791195.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            12648..13172
                     /locus_tag="ACHA34_000520"
     CDS             12648..13172
                     /locus_tag="ACHA34_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFM8791196.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            13188..14687
                     /locus_tag="ACHA34_000521"
     CDS             13188..14687
                     /locus_tag="ACHA34_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFM8791197.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(14775..15377)
                     /locus_tag="ACHA34_000522"
     CDS             complement(14775..15377)
                     /locus_tag="ACHA34_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8791198.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            15481..16437
                     /locus_tag="ACHA34_000523"
     CDS             15481..16437
                     /locus_tag="ACHA34_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFM8791199.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(16742..17698)
                     /locus_tag="ACHA34_000524"
     CDS             complement(16742..17698)
                     /locus_tag="ACHA34_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8791200.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            17872..18432
                     /locus_tag="ACHA34_000525"
     CDS             17872..18432
                     /locus_tag="ACHA34_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791201.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            18425..19249
                     /locus_tag="ACHA34_000526"
     CDS             18425..19249
                     /locus_tag="ACHA34_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFM8791202.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            19259..19876
                     /locus_tag="ACHA34_000527"
     CDS             19259..19876
                     /locus_tag="ACHA34_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8791203.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            19894..20577
                     /locus_tag="ACHA34_000528"
     CDS             19894..20577
                     /locus_tag="ACHA34_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM8791204.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            21260..22495
                     /locus_tag="ACHA34_000529"
                     /pseudo
     CDS             21260..22495
                     /locus_tag="ACHA34_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            22503..23390
                     /locus_tag="ACHA34_000530"
     CDS             22503..23390
                     /locus_tag="ACHA34_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8791205.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            23407..24243
                     /locus_tag="ACHA34_000531"
     CDS             23407..24243
                     /locus_tag="ACHA34_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8791206.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            24246..24869
                     /locus_tag="ACHA34_000532"
     CDS             24246..24869
                     /locus_tag="ACHA34_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791207.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            24866..26158
                     /locus_tag="ACHA34_000533"
     CDS             24866..26158
                     /locus_tag="ACHA34_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8791208.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      26517..26614
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            26730..27311
                     /locus_tag="ACHA34_000534"
     CDS             26730..27311
                     /locus_tag="ACHA34_000534"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFM8791209.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            27314..28621
                     /locus_tag="ACHA34_000535"
     CDS             27314..28621
                     /locus_tag="ACHA34_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFM8791210.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            28914..29402
                     /gene="purE"
                     /locus_tag="ACHA34_000536"
     CDS             28914..29402
                     /gene="purE"
                     /locus_tag="ACHA34_000536"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFM8791211.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            29395..30519
                     /gene="purK"
                     /locus_tag="ACHA34_000537"
     CDS             29395..30519
                     /gene="purK"
                     /locus_tag="ACHA34_000537"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFM8791212.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            30532..31827
                     /gene="purB"
                     /locus_tag="ACHA34_000538"
     CDS             30532..31827
                     /gene="purB"
                     /locus_tag="ACHA34_000538"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFM8791213.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(32007..32384)
                     /locus_tag="ACHA34_000539"
     CDS             complement(32007..32384)
                     /locus_tag="ACHA34_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791214.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            32640..33368
                     /gene="purC"
                     /locus_tag="ACHA34_000540"
     CDS             32640..33368
                     /gene="purC"
                     /locus_tag="ACHA34_000540"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFM8791215.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            33368..33622
                     /gene="purS"
                     /locus_tag="ACHA34_000541"
     CDS             33368..33622
                     /gene="purS"
                     /locus_tag="ACHA34_000541"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFM8791216.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            33624..34295
                     /gene="purQ"
                     /locus_tag="ACHA34_000542"
     CDS             33624..34295
                     /gene="purQ"
                     /locus_tag="ACHA34_000542"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFM8791217.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            34296..36518
                     /gene="purL"
                     /locus_tag="ACHA34_000543"
     CDS             34296..36518
                     /gene="purL"
                     /locus_tag="ACHA34_000543"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFM8791218.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            36503..37942
                     /gene="purF"
                     /locus_tag="ACHA34_000544"
     CDS             36503..37942
                     /gene="purF"
                     /locus_tag="ACHA34_000544"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFM8791219.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            37965..39017
                     /gene="purM"
                     /locus_tag="ACHA34_000545"
     CDS             37965..39017
                     /gene="purM"
                     /locus_tag="ACHA34_000545"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFM8791220.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            39014..39592
                     /gene="purN"
                     /locus_tag="ACHA34_000546"
     CDS             39014..39592
                     /gene="purN"
                     /locus_tag="ACHA34_000546"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFM8791221.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            39589..41124
                     /gene="purH"
                     /locus_tag="ACHA34_000547"
     CDS             39589..41124
                     /gene="purH"
                     /locus_tag="ACHA34_000547"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFM8791222.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            41140..42414
                     /gene="purD"
                     /locus_tag="ACHA34_000548"
     CDS             41140..42414
                     /gene="purD"
                     /locus_tag="ACHA34_000548"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFM8791223.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            42536..43450
                     /locus_tag="ACHA34_000549"
     CDS             42536..43450
                     /locus_tag="ACHA34_000549"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFM8791224.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(43583..45148)
                     /locus_tag="ACHA34_000550"
     CDS             complement(43583..45148)
                     /locus_tag="ACHA34_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8791225.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(45141..46592)
                     /locus_tag="ACHA34_000551"
     CDS             complement(45141..46592)
                     /locus_tag="ACHA34_000551"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8791226.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(46606..47622)
                     /locus_tag="ACHA34_000552"
     CDS             complement(46606..47622)
                     /locus_tag="ACHA34_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8791227.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            47842..48930
                     /gene="chvE"
                     /locus_tag="ACHA34_000553"
     CDS             47842..48930
                     /gene="chvE"
                     /locus_tag="ACHA34_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8791228.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            48984..50510
                     /gene="mmsA"
                     /locus_tag="ACHA34_000554"
     CDS             48984..50510
                     /gene="mmsA"
                     /locus_tag="ACHA34_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8791229.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            50512..51717
                     /gene="mmsB"
                     /locus_tag="ACHA34_000555"
     CDS             50512..51717
                     /gene="mmsB"
                     /locus_tag="ACHA34_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFM8791230.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            51974..52951
                     /gene="guaC"
                     /locus_tag="ACHA34_000556"
     CDS             51974..52951
                     /gene="guaC"
                     /locus_tag="ACHA34_000556"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFM8791231.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            53215..54639
                     /locus_tag="ACHA34_000557"
     CDS             53215..54639
                     /locus_tag="ACHA34_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8791232.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(54681..54938)
                     /locus_tag="ACHA34_000558"
     CDS             complement(54681..54938)
                     /locus_tag="ACHA34_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791233.1"
                     /translation="MPIRILTALVNKITIRKAFYFSSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            55100..56209
                     /gene="pdhA"
                     /locus_tag="ACHA34_000559"
     CDS             55100..56209
                     /gene="pdhA"
                     /locus_tag="ACHA34_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFM8791234.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            56212..57189
                     /locus_tag="ACHA34_000560"
     CDS             56212..57189
                     /locus_tag="ACHA34_000560"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFM8791235.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            57214..58857
                     /locus_tag="ACHA34_000561"
     CDS             57214..58857
                     /locus_tag="ACHA34_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFM8791236.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            58865..60271
                     /gene="lpdA"
                     /locus_tag="ACHA34_000562"
     CDS             58865..60271
                     /gene="lpdA"
                     /locus_tag="ACHA34_000562"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFM8791237.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            60433..60708
                     /locus_tag="ACHA34_000563"
     CDS             60433..60708
                     /locus_tag="ACHA34_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFM8791238.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            60714..61481
                     /locus_tag="ACHA34_000564"
     CDS             60714..61481
                     /locus_tag="ACHA34_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFM8791239.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            61649..63478
                     /gene="typA"
                     /locus_tag="ACHA34_000565"
     CDS             61649..63478
                     /gene="typA"
                     /locus_tag="ACHA34_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFM8791240.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            63739..65511
                     /gene="recQ"
                     /locus_tag="ACHA34_000566"
     CDS             63739..65511
                     /gene="recQ"
                     /locus_tag="ACHA34_000566"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFM8791241.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            65625..65927
                     /locus_tag="ACHA34_000567"
     CDS             65625..65927
                     /locus_tag="ACHA34_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFM8791242.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            65930..67093
                     /locus_tag="ACHA34_000568"
     CDS             65930..67093
                     /locus_tag="ACHA34_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8791243.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            67119..70547
                     /locus_tag="ACHA34_000569"
     CDS             67119..70547
                     /locus_tag="ACHA34_000569"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFM8791244.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            70568..71692
                     /locus_tag="ACHA34_000570"
     CDS             70568..71692
                     /locus_tag="ACHA34_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFM8791245.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            71673..72119
                     /locus_tag="ACHA34_000571"
     CDS             71673..72119
                     /locus_tag="ACHA34_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8791246.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            72103..72441
                     /locus_tag="ACHA34_000572"
     CDS             72103..72441
                     /locus_tag="ACHA34_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFM8791247.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            72560..73120
                     /gene="rsmD"
                     /locus_tag="ACHA34_000573"
     CDS             72560..73120
                     /gene="rsmD"
                     /locus_tag="ACHA34_000573"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFM8791248.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            73117..73608
                     /gene="coaD"
                     /locus_tag="ACHA34_000574"
     CDS             73117..73608
                     /gene="coaD"
                     /locus_tag="ACHA34_000574"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFM8791249.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            73598..74641
                     /locus_tag="ACHA34_000575"
     CDS             73598..74641
                     /locus_tag="ACHA34_000575"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFM8791250.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            74800..75528
                     /locus_tag="ACHA34_000576"
     CDS             74800..75528
                     /locus_tag="ACHA34_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFM8791251.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            75644..75889
                     /locus_tag="ACHA34_000577"
     CDS             75644..75889
                     /locus_tag="ACHA34_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8791252.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            75886..76104
                     /locus_tag="ACHA34_000578"
     CDS             75886..76104
                     /locus_tag="ACHA34_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFM8791253.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            76240..76779
                     /locus_tag="ACHA34_000579"
     CDS             76240..76779
                     /locus_tag="ACHA34_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFM8791254.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            76772..79045
                     /locus_tag="ACHA34_000580"
     CDS             76772..79045
                     /locus_tag="ACHA34_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFM8791255.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            79083..80129
                     /gene="holA"
                     /locus_tag="ACHA34_000581"
     CDS             79083..80129
                     /gene="holA"
                     /locus_tag="ACHA34_000581"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFM8791256.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(80218..80472)
                     /gene="rpsT"
                     /locus_tag="ACHA34_000582"
     CDS             complement(80218..80472)
                     /gene="rpsT"
                     /locus_tag="ACHA34_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFM8791257.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(80872..81141)
                     /locus_tag="ACHA34_000583"
     CDS             complement(80872..81141)
                     /locus_tag="ACHA34_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791258.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(81403..81531)
                     /locus_tag="ACHA34_000584"
     CDS             complement(81403..81531)
                     /locus_tag="ACHA34_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791259.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(81611..82066)
                     /locus_tag="ACHA34_000585"
     CDS             complement(81611..82066)
                     /locus_tag="ACHA34_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFM8791260.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(82204..84132)
                     /locus_tag="ACHA34_000586"
     CDS             complement(82204..84132)
                     /locus_tag="ACHA34_000586"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8791261.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        27483 a        14397 c        18141 g        24289 t
ORIGIN      
        1 cgttttttgt aataaataca gtaatctatg aaatgaaaag ggttacgttt aataaaactt
       61 gcttttttgt ttttgttcac cataaatatt tatttaagaa agtattaacc tgcatttttc
      121 tctataaaaa agatttttta aatagataaa aaaggaggat taaatgagta tttgatagta
      181 tttatattaa aattacacat gtaatttaaa aacgcttgac attgtgtttg agatcactta
      241 cttttagcat gtaaaagtat tggattaagg agctatgcaa atgaaaaaga aatttattac
      301 tggtgggatt gtcgttgcat ttctatgtct aattggctac acaggcacgc ggtacgcaaa
      361 tgaaattatt ttgtggggtg gagagacaga tattgataca atcaatgaga atctgacaca
      421 gcttgatcat acattgacag ataaagagca agcgcttaca gatcttaccg ctcgactagc
      481 acaaaaagaa cagacattgc aagattcaaa ggcagatgtc caagaatacc aaacaaaagt
      541 aaatgaatta gaaaaccaaa aaaatcaatc ggcagcagaa aaacaaaact tagaaagtca
      601 attagtcagc aaggatgcac aactacaaag caaacaaaat gaaatcaatg tcaaactaga
      661 tgagatcaac caaaaaaatg atgaaatcaa tcatctgaat gcagattgga cagcaagact
      721 acaagaagca caagcaaaac tttcagaatt acaaaatact gtaaatggtt tgaatcaaca
      781 aatcgcaaca ctgacacaag aaaaaacaaa tgtagaaact cagttaacag atacacaaaa
      841 gaaattagac gaagcaactg ctgaaaactc aagcttgaaa caatatattc aaaagttaga
      901 aaaagcaaaa gatgaagtgg aagatacagc tgataaatct cagcaaattg ttgaagaaca
      961 tttgcgtaaa taatctttct taacaagtaa acctaaatta gtaaagaagc aacagtaatc
     1021 tatactgctg cctctttttt ttagtacatg tccttcatct tcgcaggaag tgccaatatg
     1081 attttaagaa aggtaataga caaaaagtca aaaagataaa acagagtttc ttctttacaa
     1141 agggaatgta tgttcgtata atggtattga ggtgaaggtt atggagaaaa aatatggaac
     1201 agtagataag atccgtactt tgcaagttag tgccagaccg ttgatccgtt tcagtttaaa
     1261 tggggtcaat tgtttgattg cttcccatag tttgaacttc ctagctgaag tagacgaagg
     1321 gatgaagtta gtcgttacag gttattataa taatagaaag caatttgttg tgagatccta
     1381 tcatgtgtta gggaaaccca aaattgtggt agaatatgag aaaagtctat acccaagaaa
     1441 aaaggtccga caagatgaaa gaataaacta gaaaacaagc ggctgagaca aaaatgtcct
     1501 agccgcttgt tttggaaaga tggatgcaga cgatgggatt tgaacccaca cgagcatacg
     1561 ctcacaagaa cctcaatcta tatataacta catgtattta agtatttgtg atgaatcact
     1621 aaactttcca aactcatttg agggatattg cttaaagatt gaacagatca attcgaagcc
     1681 ctattagtat tcgctcaatg gggggatcag aggccataaa aagagactag tcattactag
     1741 tctcaggtag tggaattgta ggattttaaa cgttcctgga gagtaccggt atgtaactcg
     1801 aagaggtgat tatcatagtc gtaaaagtaa attgaagaag cctcgccttt cacacgagaa
     1861 cgtcctggtt cgacagttaa acctaacatt tggatttttg agacaaacga atctatatct
     1921 tgttcgtcaa ttttaaaagc aatatgattg tatgtctttg gcagtttatt cgacgaattc
     1981 tgcattactg caatccatag gtccttaact aagaaaaatt tttctggata taatgaaaat
     2041 ttttttgttg actatcgtag atttcttttg catcaaataa ggtatttaac aattaagtat
     2101 ttaaaagata acttttaagt tatctttaag taatgcttaa agctatgtta tactctatta
     2161 ggaaatataa gaaggagtat aaaaatgaac aaacataata gaaaaataac gaataacaaa
     2221 gaactgatct tgttactaat agggatttgg ggtatctttt tactctttaa tatgtatatg
     2281 ccgactatga gagcggatga cgtagtttat gcaagtagat tagatgaact tggctatttg
     2341 ggtgcttcga tagaacatta taagacatgg agttcaagaa ttattataga actattctta
     2401 atgttctttt ctaaacactt tatgctatgg aaattattaa attctacgat tatgttagga
     2461 attgtagtat tactttgcaa gtatgttttt gagaaaataa atgttaaaaa tttattattg
     2521 gtttgctcta tatactgttt aataccattg acagtaatgg gggaaaccgg ttggagagca
     2581 accaccttga actatcagtg gccagtagca tttagtttac taacttttta tccgtttttt
     2641 caactcttaa gaggagaaga aataaataga aaaatatatt gggtaagtat tcctttattg
     2701 atatttttaa ctaatcaaga acaagtcaac gcctgttttt ttgttctaac aagtatagtt
     2761 agtttatatt tgattgtcaa tggacgatat aattataagt tatcagtatt ttcaataata
     2821 agcttagcag aattaatttt ctctttgaca actccaggga atgcattacg tgctgcacat
     2881 gaaattaata aatggtttcc tgaatataaa aatttcaact ttcttaataa gttagattta
     2941 ggtatttcat catttggaaa gccgtttttt ttagacatga atatcttatt tttattgcta
     3001 tttttcttaa ttttccttct aacatatagg aaatgtcaaa actattatgt gcgtatattg
     3061 actgcattac cgtttttctt aaacctgatt atttattttg gtaatacaat gggtcaaagc
     3121 tttacttacg tcaatggaaa taaaagggca atgatttgga gtagtagtaa cctgaataat
     3181 ctctttacag agttaggaac gaaactgtca cttttttatc caggtacttg gatagctact
     3241 ttagtagtct tggcattatt actatgttta attgttggaa tttatttaag ttttgacaat
     3301 aagaaaactt ctattttttt agttattttg atgataatgg gattttgttc aaggttaatt
     3361 atgggatttt ctcccacagt atgggcttcc ggaatgcgga cgtactatat actttatgtt
     3421 gttatcgcca tcttagtact aatggcagtt aaagagctaa tgaaaagcat gagtgtacaa
     3481 aaaaatgaat ttatgcaatt tggattaacc gtgcttggta tttgtacctt tataataacg
     3541 gtgataaaca gataatatac aaaaaatccc tcgctttagt attaggtgag ggattttact
     3601 cgtttttcga ttaattgttt taacacttgc aagtttctac gagtagcttg tttccgacct
     3661 tcatatactc acctcaggct gtttacatga ttgtacgttc tttttgtaaa ggatacagtg
     3721 atatgtatat aatggcataa agaaacgttc aaacaaaaaa actcgtgaag caaaattaac
     3781 ttcacgagtt gtagtgcaga tgatgggact cgaacccaca cgagctaacg ctcacatgac
     3841 cctcaatcat gcttgtctgc caattccaac acatctgcgt aataataaca cactaatgat
     3901 agcgaggttt tacatagttg tcaagctcaa aaatgggagg acttattaag atggagaaat
     3961 atgacggaga attttcagga ctgggaatga tcttaggtgt attgattgga cttgcattcg
     4021 gacgtttttt gcttggtttt atgctaggaa tcatttgtgg aattgcaatg gattgggcag
     4081 cgaatctatg gaatgactat catgacaatt aagttctcaa cgcttgtttg tgttttttta
     4141 atacttacgt ttttcctaaa taagtggcaa aattttctag gatggagtat gatattgaaa
     4201 tagtaaataa caaaaaattt ggaaagttct aactttttga aatgagggat tggctaagtg
     4261 atgagagaat ttgaaaaatc caacaaatta gaaggtgtca gttacgatgt tcgcggtccc
     4321 gttctagaag aagcggagcg gatgcaagaa gagggcatta gtattttaaa attgaacacg
     4381 ggaaatcccg caccttttgg atttgaagca ccaaacgaag tcattcgtga tttgatcatg
     4441 aatgcccgtg actcagaagg ctactcagat tccaagggga tcttttctgc tagaaaagca
     4501 attgagcaat attgccaact gaaacatttt ccgaatgtga cgatcaatga tatctacacc
     4561 ggcaacggag tgagcgaatt gatcacgatg tgtatgcagg ggttgctgga caatggagat
     4621 gaagtactgg ttccaatgcc ggactatccg ttgtggacgg cttctattgc attagctgga
     4681 ggcacacctg ttcattatat ttgcgatgaa gaggcagaat ggtatcctga tattgatgat
     4741 atcaaatcaa aaataacgag tcggacaaaa gctattgtta tcattaatcc taataatcct
     4801 actggggcac tttatccaaa agaattatta gaagaaatcg ttgaagtagc tcgacaaaat
     4861 aacctgatta tttattccga cgaaatctat gatcgcttag tgatggatgg tctagaacat
     4921 attccgattg ctacattagc gcctgatttg ttcgttgtta cgctgaacgg tctatccaaa
     4981 tctcatcgtg tagcaggatt tcgagtaggc tggatggtcc taagtggaga taagtcacac
     5041 gtcaaaggct atatagaagg gctgaatatg ttgtcctcca tgcgtttgtg ttccaatgta
     5101 ttgtctcagc aaattatcca aaccgcgcta ggcggttatc aaagtgtcga tgagctgctc
     5161 ctgccaggcg gaaggatcta tgaacaaaga gaatatatct ataatgcaat caatgatatt
     5221 cctggtcttt cagccgtaaa accaaaagct gctttttata tattcccaaa aattgataca
     5281 aagcgattca atattttgga tgatgaaaaa ttcgttttgg actttttaca tgaacatcat
     5341 attctacttg tacatggtgg tggatttaat tggaaccagc cagatcattt tcgaatcgtt
     5401 tatcttccta aaatggatga tttgaaacaa acagctaaga aaatgagaga attcctagca
     5461 acctatcgcc aaaaatgatt ttaaaaacca aatttttcag taggagggcc acagacaaac
     5521 acactgaggc tctctttcta gtggagtttt cttaaataac taaagtatat tatttagttg
     5581 caagtctttt cttgaagttt gcgacttcct aaagtaaaat acgaatagaa tgcgaatacg
     5641 catattttgg ggaggatact tatggaaacg ttaatcgagt cctttggaac gatcattaaa
     5701 gagattcgaa aagaacaaaa aatgacgcaa aaaatgcttt cgcatgatat ttgttcacaa
     5761 agtgtattaa gtcggatcga aaataatgag gaactgccga atgtattggt gatgatgcag
     5821 atttgtcaac ggctaggggt aacgattgat catgtgatga atctctctcg aaaaactact
     5881 cggacaaacg ataaattatt tgaactgttg gatagttgtt tccaaaaacg tgattatcgg
     5941 aagttagaac aagtactaaa atcttctgat atttctgaaa atctgtatca agcaactgac
     6001 tttcaacgct attattatta tctcggtgtt tgcgaattca cgttgcgtca aaatatctct
     6061 cagtcccttt attatttgaa agaagcatta agctactcta gccaaaaaga aagaatccat
     6121 gtttctgaca cagaaataca actgattagt tgtatcggaa aaatatatgg tgtggcagga
     6181 aaaacggctg aagcacgcta ctatttgagt cgaagcatcc aactgctgca tcaatcagct
     6241 gatgaacgat caaaaagtga attttcgcaa attttttata attatggaaa tttcttattc
     6301 catcaaaatg aaatcgacga agcgttagaa caggtgaatc aaggaatcta ttgggctcaa
     6361 gagaagaata gctactatta cctcaatgat ttatttgtgc ttaaaagctt aatatacaag
     6421 cgtaaagaag aaccagaaag agcagcattc tatgaaggct tagcacaagc tgtgaaacaa
     6481 atttcaaaaa atttgtaaat ggtgtagacc attcttgact tcttctctaa ataagagtac
     6541 tttagaggta gaaaaacaaa gggagagaat agaatgagaa cttttatcta tgctgacaaa
     6601 ttctttttag cgtcaggtgt caaacatgca ggctatcttg aaattaccga tggacttttt
     6661 ggtgaataca ccaaagaact acctgaggga gaagtcaaca tcattgacca aagcggcaaa
     6721 tggattgctc ctggtttggt agatacacat attcacggct atatgaatca tgatgtaatg
     6781 gataatgatg cagaaggcat caaagtgatg tctgaaggtc tgttatcttg tggagtgact
     6841 tcatttttac caacaacatt gacgtcaagc aaagaacgtt taaaagatgt agctgagaca
     6901 atcggcaaaa tgtatcagga agcaccagga gctaaaatca aaggaatcta ttttgaaggt
     6961 cctttcttca cagaagaaca caaaggggca caaaacccaa gttatttcgg ggatcctgat
     7021 ttggatactt tccatgaatg gcaagaagct tcaggtggtt taatcaaaaa aattgcttta
     7081 gcaccggaac gtcaaggtgt agaagagttt gtcaaacaag tgactgaaga aggagtagtt
     7141 gtttctctag gacacagtaa tgctacacta gaagaagcgc aaaaagctgt tgaagcaggc
     7201 gcaagtgtat ttgtccatgc tttcaatggt atgcgtggat tgaatcatcg cgaaccaggt
     7261 atggtgggtg cgttattatc tttagaacat gtcttttctg aattgatttg tgatggccat
     7321 catgtacatc caaatgctgc gggaatccta atggaaaaag caggacatga tcatgtcgca
     7381 ttaattacag actgtatgat ggccggtggg atgcctgatg gaaattacaa tttaggcgaa
     7441 tttccagtag tagtaaaaga aggaacagca cgtctagaat ctggcaactt agctggaagt
     7501 atattgaaat tgaaagaagc aattaaaaat gttgtagact ggggattagc aacaccagaa
     7561 caagcagtca tgatggctag tctgattcca gcaatcagct gtaaaatcga tgatacatgt
     7621 ggaaaaatcg ctcaaggacg cgatgcagac tttatcgtat tgaaccctga tatgacattg
     7681 gaagcaactt atctagatgg cgtggaacgt ttccacgcat aatataattt aagttaacct
     7741 taaaactaaa cttcttaagt ttggttttaa ggtttttttg tgttctagta aaaatcactt
     7801 tacataagtt tcaaaagttt ctttaatttc ctattgttca ataagtaaca atgtaaagat
     7861 aaaattaaaa atgttaatac gtattagcaa aataaaagct ttttttattg tgaataaatg
     7921 aacataatta ggaggagaaa gatgaactta tttgacattt ttcgatcgac tttgacagat
     7981 atgtcgattg tcagtgcaat cacttcaaca gtttttatta tattattagg gtttgtttgt
     8041 agaaaaaagg ggattttttc tgaatctgtt ggaaaaattc tatcgaaagt cgtcttaaca
     8101 gtggctttgc cagcactggc atttcgagct ttcatgcaag atatcaatac agaattactg
     8161 atgcaaggaa tcaatgtctt gatttgggga ttagttattt atattattct tattctagtt
     8221 tcaaaacctt ttttcttaag ctataaaggg gataagcaaa caacgttgcg tgtcctgacg
     8281 atctttggtt ctacgacctt tttcggaacg cctatcgtca gtgcgattta tggtcctgtc
     8341 ggcgtgatgt attcctcaat tttcaatatt ggctaccgaa tctttttgta ttcttatgga
     8401 tacataaaaa tgagtggctt gaagatggaa gctaaaaata tcaaagcgat gttcgtcaat
     8461 ccaatcatta tcgctacttt tgccggcctt ttcatttggc tgttccaagg gtatctgcca
     8521 caagtgtcag ttacaggtgc agaaggagag ataagccaag tagcgttttt gcgaattgac
     8581 cagactgcac cgtggctatt tcagccgatg gattatttag ctggtttaag ttcaccattg
     8641 gcgtggctag caatcggcgc aacattaggt gaagtaagct tcaaaaaagc tgcatctgaa
     8701 aaaacttcat ggtattacag tctagtcaaa gtggtatttg tacctgcatt gaatatcgtg
     8761 attctagcag ttttgacggc aacgaatatt ctaccagtca attttgaagc actggcaaca
     8821 atcgttatca tgatggctac accagctgca acagtggcag cagcctatgc tatcagtttt
     8881 gatagggagg cacttttagc ctccaatgca tccttactgt caactatttt tgctgtggta
     8941 ctgacacctg tatggatcgt tgtattacaa atgattcatg gcataggttt attttcataa
     9001 gaaatggagg aagaaagtat gagaatcgca tgttacggtg ttcgtccgaa cgagatagat
     9061 ttttttgaaa aattgaatat ttaccattat gatctcagtc tttatgaaga actgttgaca
     9121 catgacaata tcgtgtcagc aaaggaccat gatgctgtat tgttgagggg aaattgtata
     9181 gcagatgaga ccaatcttgc aaaaatgcag gaatatggga ttcgttatgt ttttacacgg
     9241 actgtgggag tcaatcatat cgatctacaa gcggcagctg attttggtat gacagtagcc
     9301 cgtgtaccat cgtattcgcc aaatgcgata gcagagttat ctttgacttt tgccatgatg
     9361 cttttgagaa atactgcgta tacgacgatc cgaacatctt tcaaagattt tcgagtggat
     9421 gagcaaatgt ttagtcgtga aatcagaaat tgcacagtag ggatcatagg aacaggcagg
     9481 atagggttga cggaagctgt tttgtttaag ggactaggag ccaaagtgat cggctacgat
     9541 attcatcaga cagaagctgc taaaaaagta ttgacgttcc actcgcttga agagttatta
     9601 gccaaaagcg atattgtcag tattcatgta cctcatatcc caggtgaaaa cgatcaaatg
     9661 atcaatgaag gatttttagc tcaaatgaaa aaaggtagca tcttgattaa tacagcccgt
     9721 ggagagcttc aagataatga agctatttta agagctttga aaagcaatca tttagctggt
     9781 tttgcaaccg atgtttttgc aaatgaaaca gaggtttttt tccgatcctt caagccatgg
     9841 gagacgattc cagaccctgc tatccagcaa ttagtagaaa tgtaccctag agttcttgta
     9901 accccgcatg taggttcaaa tacagatgag gcattatcaa atatgatttc gacaagtttc
     9961 gagaactttc gtgaaataat agaaactgga aaaacgaaaa atgaggtatc tcttcctaaa
    10021 gcccgtcaat taagataaga aaacatggat attcgtcaat aaaatgttta aaaatagtcc
    10081 aagtaaacag ttgttacttc tgtatacttg gactattttg tatgggataa ttcgatgact
    10141 caacagtaat ctgtgagctt tttccgccca ttctttgtca attcataaca ggttagcggc
    10201 tggccgattt ccaaatattt gattctttcg ttcaatatat cttgttctac taaaaaattc
    10261 aaatatttct tgacggatat acgtgacagt gagctttttt gagccagttc ttttgttgaa
    10321 aaaatagtat cttcttttag aataaatgca cttatttttt ttaaagtcag ttttgacaga
    10381 cctttaggca aatcgatgaa tgatggtatt tcgtgaatgg gagtggtgtg gttgaatact
    10441 tgatcgatca atttctgatc ggctttttca gtcgtttgga ttgtctgaga gaaagcaaga
    10501 tacttttcga tcgccagctg aaaacgttgc aaagtgaacg gtttgatcaa ataatctaag
    10561 gcaccgtagc ggatcacatt ctttatggtt tcgatatcat ctgcagcagt gatcaaaatc
    10621 actgggatat catttccttg ctgtctaagc caagataaaa aatggatacc actttcgcca
    10681 ggcatgtata catcaagcag cagcagatct gcgttttttt gtctgaaata tttttttcct
    10741 tcttctacat ttctgaaact ttcgattacg ttcatattag ggatgctttc cacaaaagta
    10801 cgattcaatt ctgcaaccat tggatcatct tcgattatca ggacatttgt catcattttg
    10861 atcttccttt tttggtaatt ctagatagac gattgtaccg ccatttgttt gtttggaaaa
    10921 ttctaaaaat ccaccgtggc gtttgacgat tgaatggatg agattcaatc caaacccacg
    10981 attgattccc ttcgtcgaaa aacctagcgt aaatattttt tctgagattt cttctgatat
    11041 cccgacaccg ccatcgcaga cggtcacgat gaatacttca gcttctgtgt catagtttaa
    11101 atgaatccgg acctgtccgt ttctttcatg tttttgtagc acggcttctt ttgcattatc
    11161 aagtaagttc cctaacactt gcaaaagtga atggatggag tcatccatct ccattttagg
    11221 gataaaagaa tcgctttcta acagcatttc aacagattgt tctttggctt cgtttatttt
    11281 gcctaataaa aaaccggcaa ttgcaggtac tttgatcaga cttgagactt ggccaatctc
    11341 ttttttataa ttttgattta gctgctggat aaaatgggtg acttcttcat acttcttcaa
    11401 atcaatcaaa ccagaaatga catgaagttt attcatgaat tcatgggttt gagctctcat
    11461 ggagtttata tattgtttcg tccccccaag ctcctccatt aatttcatta cttctgatcg
    11521 atttcgtaaa gttgcgactg ctcctaaaaa acggtcattg ctaaaaagag gtgtaacgct
    11581 agaaatagtt tctatcccat ttaaatacat ggattgagtc cgaacggtcg tttgatgctc
    11641 aatcgtctct ttaaacaaaa cgtcatatag attatcactg atttttttcc tacttcgaaa
    11701 tccaatagct gctctgtttg gtagaaaatg ttttttgcag gtgtattgat caagaggata
    11761 gttttatcag aagagatggc taagattcct tcatctactt cattttcgat aatcgttttt
    11821 tcacttaact gttctgctat ttcttgtggt tcaagtccaa gcagtacttt tttcattttt
    11881 tgggcaagaa aaacggcgcc tatgatgcct acgctaagtc caataatcaa tgaaaaaaca
    11941 atcatttttt gtccatcttt gacttgattg tcaatcgtgt tgtttgttaa gccaacacaa
    12001 actgcaccaa taatctcttg atcataataa atagggacga aatagcggaa gccgaaccct
    12061 aacattcctt ctttttcaga gaaaaattct tgtcctccaa tcactttttt cacatcttca
    12121 tgacttgaga aaggaagacc gatgtaactt gggtctgcgt gagaatatcg aatgccttca
    12181 gggtccatga caacgataaa atctaaattc gatgcaatca tgatttcatt tgttattcct
    12241 tgaatcgcat gtgtcgggtc accagtctgc agcttttttt ggatgagttt atcattggca
    12301 acgattttag caatgctaga gattttatct gacacactag atcggttcat cgaaagaata
    12361 aaattcctga tcaatatggc agaaacaatc aaagaaacga atgtggagaa acaaacgaca
    12421 aggatgatcg tgttctggag ctttaatggt tttttcttaa aaaaaggaaa cataggctac
    12481 ctcgaaagaa tttattgtga aaattttaac atgaaaacaa aaatttaaca aattgtcaac
    12541 gataaacaca accttcctat aaaaaacacc actgagtcgg taaaagaagc tatacagaat
    12601 tttcaaacat tggtatgata gacgtagaaa tggaaggagg cgtgtatatg atagggggaa
    12661 tcgcacttga gacgatttat tggtatacat tgctgatttg tgcaggattg gcagtcctat
    12721 tgattatttt tggagatgta ttcgattttg atggcccgct tgatccgatg cttattatcc
    12781 cttggttaac tttcacaagc ttatttggtt ttctaggtga gcgtttaaca gatcaatcat
    12841 ctttattttt atttattgct agtggaggca tatcgatagt gttggtattt ttgcttaatt
    12901 tctatatatt gatgccgatg aaacatgccg aatcaacgat gtcgtcttct gaaaaaactc
    12961 tggaaggaca agtagctgtt gtgatcacac cgatccctgt acgaggtatg ggagaaattc
    13021 agttgaaaag tgttaccggt tccattagtc gtccagcttg tttttacagc ccccaagaac
    13081 agacagctga gcggggaacg aaagtattga tcattgagat caaggaaaga atctgttatg
    13141 tcatgcctta tgaaagcatg ataaaagctt aaaggagcgt gtgttaaatg gaaataccag
    13201 ttgtttttat cgcgatcgca atcgcagctt tgattttact gatgttgctg atcgtctttg
    13261 tagcgaagta ccagacagca aaaccggatg aagcgttgat catcagtgga agttatttag
    13321 gaagcaaaaa tgtgcacatt gatgaaagca ataacaaaat caaaattgtg cgtggaggtg
    13381 gcgcattcgt cttgccagtt tttcaacgtt cgaatcggat cagtctatta tcgagtaaat
    13441 tggacgtctc cacccctgaa gtgtataccg aacaaggcgt accggtgatg tgtgatggta
    13501 cttcgatcat caaaatcggt tcatctgtag aagagattgc tacagcagca gaacagtttt
    13561 taggcaagac aagagaagaa ctggaaaatg aagcaagaga agtattagaa ggacacttgc
    13621 gctcgattct tggttctatg actgtagaag aaatctatca aaatcgcgat aagttcagtc
    13681 aaagcgtaca agaagtcgct tctgtagacc ttgcaaaaat ggggttgatc atcgtttctt
    13741 tcactatcaa agaggtcaga gacaaaaacg gttatcttga ctctttaggg aaaccaagaa
    13801 tcgcccaagt caaaagagat gcagatatag cagaagcgga agctctaaaa gaaacgcgta
    13861 tcaaaaaagc tcaagcagaa aaagaatcac aagcagctga attacagcga caaacagaga
    13921 tcgcagaatc attaaaagaa aaagaactga aattagcaac ctacaaacaa gaacaagatg
    13981 ttgctaaagc aaaagcagac caagcctaca atttagaaag tgcccgagca caacaacaag
    14041 taatcgaaca ggaaatgcag attaaaatcg tagaacgcca aaaacaaatc gaactggaag
    14101 aaaaagaaat cacacgtcgt gaaaagcaat atgattcgga agtgaagaaa aaagcagatg
    14161 ccgatcggta cgctaaagaa caagaagcac aagctcaaaa agtcaaagaa gtgactgaag
    14221 ccgaagcaga acgattccgt gtggaagcac tggcagaagc ggaagccaat aagacacgtt
    14281 tagctggaca agcagaagcc gaagcagcac tagcaaaagg aaaagcagaa gccgaagcaa
    14341 aacaaaagat cgccaatgcc ttcaaggaat atggcgaagc agctgtcttg agtatggtta
    14401 tcgatatgct accgcaattg atgcgcgaag cagcacagcc gctaggaaat atcgaaaaaa
    14461 tctctgttgt cgatacagga agcagttcag gagaaaccag tggagcgaac cgagtgacaa
    14521 attatgccac aaatttgctt tcttctactc aagaaacact aaaagcgaca acaggacttg
    14581 acttgaagga aatcgtagag aatttgtcga cacgtgggac tactagccaa gtaaatatgt
    14641 atgaagcgcc aaaagaggca gaacaaaact cgatgaagga agaataaagt agtacacttc
    14701 accgttcaat caaaaatgga ggaagacttt ttaaagttct tctctccatt tttttatttt
    14761 attgcaaaag aaaattagtc ctcgctattt ttcttttgtt tttggatctt agcttctatc
    14821 tcttgtaaat ttgttcttag caatcgttct ttcttttgga gttcgaccaa atcttctgat
    14881 agttcttcct tcttttctgt ttcaggagga cgaagaaaga aattgatacc gattgcaatg
    14941 aatgtcccta taaaagtatc tagtacacgt tggatagcat acaaagcgga ttcgccttga
    15001 gggatgctca acgcaatcag caacaacgta gctattgcgg aaatgatacc ggaattattg
    15061 ttaataccat ctgacacgac gatcacaata ataactagaa taggaagcaa gatcaattct
    15121 accaaaaaat cattgtgaaa atagcttttg acaaaaaaat aaatgatagc taatgtacca
    15181 cctaatgtat tgccgatgat cctagacttt ccaaaagaaa cggttgtagt caagtcctga
    15241 cgcaaagaaa aaacagcaga taatgcagca atcatcggga ttcctcgttg agtcaaatga
    15301 aaaaacaaga tacataaaaa aacagctaag gcacttttga tcgtacgcat acctagtcga
    15361 aaacggccaa attgcataga gattctcctt tttggtcttt ttactatcat acctaaactt
    15421 ttgtctgata gctagttttt cattattcaa gtataattaa aataaaaaag ggggaagtaa
    15481 atgcgtatag gaatgcgaac agtcaaaaca gtgatcagtg tcttcttttc gatggtcaca
    15541 gcgggtagct tatcgctgtt gtattggcca gctgcaggga tcattgcatt attaagtgta
    15601 ggaaatacca agcgctctac attgcgtacg ggcatgaatc gaatcgcagc ctttatatta
    15661 gctacggcaa cagcactgtt cagttttttt gctgttgggt atactatttt tggcttcagt
    15721 ctttttttat tcctgttcat cccgcttgcc tctcgcttca aattaaccga agggatagta
    15781 gtaaattcgg ttttggtcac tcattattta gtggaggaaa acatgtcttg gcagctgatc
    15841 ggcaatgaag cgggattaat ggcgattgga ttagtatttg ctttgcttgc aaatgtttac
    15901 atgccagatc gtacgaagca gctgaaggaa aaccaaatac agatcgaaaa agaatttcga
    15961 aatattttga gacaaatggc agaatttctt ttatcagaaa ataaagaaga tgtgcagatc
    16021 aaatgcgaac atttactgac attcattcgc gaaagccaag aagatgccag agaacatcaa
    16081 gaaaactact ggctcagaca gccgctatac tacgaaactt acttctctat gagaagagca
    16141 caggccaatg tgattaaaga catgctggaa aatctagaga gaatccagca accagcttat
    16201 tatggaaaac atatctatgg tttattgatt tatactgcag aaacattttc agagtcaaat
    16261 gatggcagac aaattttaac acgtattgaa gaagtttatg tattgtatcg gcaaatgcct
    16321 ctgccgacta gtcgccctga attcgaggat cgagcagaat tgttccagtt tttgcagtct
    16381 tttaaaagtt ttattgaaat caaggcagaa ttctcccaac aaaaaatcca aaaatagagg
    16441 ttgtgaattc agctaatatt caggtcaaaa agctgatttc acaacctctt ctttaaacgg
    16501 tgttctcctg tcaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgtt
    16561 ttttgaaatt gctccttgtg actcggagct gaacggtagg ctcacaacct ctttcttaaa
    16621 cggtgttcaa aagctttcct ctgcggtaat aagtccagcc aaacgaaaaa taggaggagc
    16681 tatttttgtt tggctgttct tattacttga tgcagaacag ctttttcaca accttttaaa
    16741 attacatatt tttccataga acttgcattt gatccaagat ttcgatgaca tcttttgtca
    16801 agaagagaga gcgattgggc aagtcacctt caatcatgtg taccatattc tttatttcgt
    16861 aattcattgc ttcagatgta ttcccgcttt cgatgaattc ctttgtacca tctgtaaata
    16921 agatctccgc acgatcagca cgcgggtaat cagcaatcgt cacataaccg ttctcaaagg
    16981 caacgatacc gacttttggc atttttgcct gaaaagtcaa gctgacagca gcaagttcat
    17041 tttctttgtt acgcaagata gtgacggatt gttcgtccac gcctgtttca aaaggaacca
    17101 ttgtagaggc aactacttca ggttgagagc tcaaaaagaa acgagcgaaa gatacagcat
    17161 acgtaccgat atccaacaga gctcctccag ctaattctgg attgaagaac cggtttttag
    17221 ggtctggttc tttgtaactg ccgaaaggtg cttggatcat ttttaaagcc cccagttttc
    17281 ctgtatccat tattgagcgt aactgctgat aaagtggcat gttgaagatt gtcatcgctt
    17341 ctgctaagat caaattcttt tcttctgcta agcgcatagc gtcagccagt tcttttttat
    17401 tcatcgtgat ggctttttca catagaacgt gtttgcctgc ttccaatgct gccaagatat
    17461 ggtcgatatg ctgtctattt ggtacagcaa tgtacacgat gtcgatttca gcatcgtcta
    17521 acatttcttg atagcttccg taagcttttg gaatattgta acggtgagcg aagttttccg
    17581 ctttttctga agttctggaa gcaactgctg ttagttcgct cgtctcttga ttgaagcttt
    17641 cggcaaactc atgtgcgatg gtaccaagac caacgattcc ccattgatat tttttcatct
    17701 gaaaacgctc cttcatagtt ggattgagtt tcttgtctgt ctattattat aacaaaaatc
    17761 acgaagaata aagaggagag gacttgaaac attaaaaaaa atgaaagcag cgatttaaga
    17821 ggtgcttttt gatcaaaact taagtagaat agttaaaagg aggggataaa cgtgcatcaa
    17881 gtatatattg atcttattat gagtgtatta ttagaacagt ttgaaactga aaaaaatttt
    17941 ttagatgaat acttgctgat cagtccagaa caatggcaaa aatggaaaga tggcgagaaa
    18001 aatctgccat ctgaagcgat gcagaaagta aaaaatcttt tcactgatta tgaatggatg
    18061 ctaacgcaaa agattttgcg tcaaacgatt atttttcccg aaaaacgaaa cgttgccgtt
    18121 gcagagtaca agcaaatcaa aacacgcatc gcgcaaaaat ggcttagcag caaagcagca
    18181 aaagtcgagc tgatcccttt gaaaaataat cagcaggctg gtagatacct tgacttgaaa
    18241 gtaagtattc aatatgatga atggggctat gatgatattt tgaattttcg tctgccagca
    18301 gatgttcaaa aacagatcga aggagaaaaa atcgaacttc tcgattgggt gaatgaaaat
    18361 ctggaagaaa cttacgttca tgaggcgaat ggggaacgtg aagaacaacg aaaggatttt
    18421 tcaagtgaat aaatttttag gatttggagc agttgtgatt atgatcgcag caatgggcta
    18481 tgttattttt agcgatgatg attcgcatgt tcaagccggc aatcaagcag tcgaacgaac
    18541 aagcaccaaa acaactgcta gtaaagaaaa gaacagtcaa acaaccagca gcaaggtaaa
    18601 ggacaaagaa acaaaagatc gtacgtccaa acaattgcca gatgttaaaa cagacgattg
    18661 gaagctagtt ttggttggtc cagatcacaa attagaaaaa gaaattgatg agtctactca
    18721 attggcatca ttaagtaacg gatacttgat cgacaaacgc attgctccag aatatgaagc
    18781 atttgccaag gctgctgaag cagctggatt tccattagtg atggtctcag cttaccggtc
    18841 tgtatcatcc caacaacaag ttttcgaaca aaatgtccaa gatgtgatga gtcgtcaagg
    18901 tgtatcagaa gaggaggcga caaagattac taaagaaacg attacggagc caggatacag
    18961 cgaacaccac acaggtctgg cagtcgatgt cgtcgatcaa aactggtaca acagttatcc
    19021 ttctactgta ttggatgcaa gttacggaga tcagcctgga gctaaatgga tcgcagataa
    19081 tgctgcgcag tatggattta tcgttcgtta tccaaaagga cacgaagata taactaagat
    19141 cacatatgaa ccttggcact tacgttatgt cggtaaagaa aacgctgagt atatcaccaa
    19201 acaccaattg acgctagaag aatatctgaa tgaattaaat gaaaagtagg aattgatcat
    19261 ggcgaaaaaa aagtggaaaa agcaaaagcg caagaatatc cgttggccag cagtggttgc
    19321 cggtgtagca gtgatactct tagcctttgt tttttccttg aagaatctgt cttctccttt
    19381 tactgataat cagcaagaaa atgaagcgca atcccatcag caatttattg aacggcttgt
    19441 tccgcatgcc caagaactac aagatggtta tggagtgctt cctagtatca ttttaggaca
    19501 agctatatta gaatcaaatt ggggaaaaag taccttggct agcaaataca ataatctttt
    19561 cgggatcaag gcttatgggg atcagaagaa agtaagcctg gaaacaaagg aatttgttaa
    19621 cgaagaatgg ataactatcc aaggagactt caaagtgtat gattcatggg aacagtccat
    19681 ggatgaccat acacaactgt tcgtccaagg agtagattgg aatcctgcgc tgtatgaaaa
    19741 agtgattacg gccaccaact atcaggaagc cgcacaagca ttgcaagatg caggttatgc
    19801 gacagacccc ggttatgcgc aaaaaatcat ccaggtcatc gagacgtatc aattgaatca
    19861 gtatgaccgg agatagcaaa ggagaagtgg ctgatggcag aacaatttac gccagaaatc
    19921 atgacggaat tgagaaaaga tatcgtaaga gtaccggaaa tcattttaga agcaagcggg
    19981 atcaagatat ttggaaaatt gatccgttcg atcatcttta ctacggatat tgcgatcata
    20041 cgtaatacaa atgcgaatgc agtaattgct gtgtatcctt ttacacctca tccagcgatt
    20101 actaaaagca ttattgaggc agctgacatt ccagttttct ccggtgtagg tggtggtttg
    20161 acacaaggtg ctcgttcttc ctacatgagc atgttcgcag aagcgcaagg ttcgttaggg
    20221 gtcgttttga acggtcctac gcctttagag acagtaaaag aagtttgtcg agtagttgat
    20281 attccagtta tcagtacggt aacgtcgaaa tatacagaaa tagatgaaaa gttagcagct
    20341 ggtatttcga ttatcaatat cagtgcaggt aaagaaactg cagaagcagt acgccatttt
    20401 cgtaaaaaat atcctgaact tcctatcatt gcgacaggtg gtccaacgga agaaagtatt
    20461 gctgagacca ttcaagcagg agcaaatgcc atcacatata cgccaccaag tagcggagag
    20521 ctttttagtc gtaaaatgga aaaatatcgg gaaactgaga aaaagaatca tgaataacag
    20581 gtgagaatca aaagacgagg aagagcagat agtggctctt cctcgtcttt tgtactccag
    20641 tactatttgt tcagaattca aaaaaaagag aaattttttt gtttttttac ctgtaatgta
    20701 cataagaaga gttaaaacct tttgttatta ttagcattct aacaaaaaaa tgtcttgatt
    20761 tattgaagcg ctttctatag aatgaaaaac gaataaaaac taaaaaaggc acccgacaat
    20821 ttagtagcag ctaaattgtc ggggcttgaa aagtcagcgg gaactgacca atcaagttgc
    20881 catagtcaat gcttgtgcat cgacgtgtat attgtaccta atttcataca gaattactag
    20941 ttatttttct gttttttagg gaagagcgca cacgaagaag catgcttatt tagctatgtc
    21001 aaggatattg ggcagttgtc tgatatcctt ttttctttgc gtaattatcc tcccatcatt
    21061 gaagcaagaa gtgtttttag cgatgaaaga agaatcagca ctatctaatt tttcttgaaa
    21121 gtattgagtt gttcatccta ggcagaacga taaggcaagt cttggttttt attccccttg
    21181 aaaaagatcg tttaataaat atgttctgta taaaaataca gtatattgaa aaataacaat
    21241 ggaagtagag gtgcagagaa tgaagaaaag aatgagaatc atcttagctt taagtgttat
    21301 gttattcttc ttgacggctt gtgggcaaga agatgacaaa aaaatcacgc tgaacatggc
    21361 ttggtggggt tcgcaagtcc gacacgatgc aacagtaaaa gtaatcgagc tttatgaaga
    21421 aaaaaatccc catgtgacga tcgattatga attttatgat tatgaaggat attacacaaa
    21481 attaggtaca ctagcagcat cagatgatat ctgggacatt ttccaactga acaatcaatt
    21541 tcctcaatac atcaatcaga ttgaaccatt agatgaatat gtggagcaag ggataattga
    21601 tatttctaat gcagatgaat cctatttagc aacgacgact tacgacgggc aattagttgg
    21661 actgtctaac ggggtgaatt cgtttgcgat tgcttacaat aaaaatatat ttgaacaatt
    21721 aaatatccct gaaccagctt caaattggac atgggatgaa tttgaacaaa tcagctatca
    21781 aatcactgaa gaattaggaa tctatgctat ttcaagattc gaagatttta tagctggctg
    21841 tattattcaa ataccacaag ttgagaaagg attgaatttt tataatcggg aagatctatc
    21901 taacagcttg ggatttgaaa atccagacta tctaacagat tttttccagc tgcgtaaaaa
    21961 tctggtagac aaaggggccc atccagaacc aggtggtgga gctgcaagta cgacggatct
    22021 gtcttttcag gcggttaggg atagagaagc ggcaatggtg tttctatcaa gtaaccagtt
    22081 gacagaaata ttgtcagggg ctcctgaaga tatggagatt cagttgatta atccacctcg
    22141 cagaaaagca gatggagaat ctggtatccc tttacgttct tcgcaaatgc tgagtatggc
    22201 aaaaagctca gcccataaag aagaagctgc taagtttatt gatttctttc aaaacagtga
    22261 agaagccaat gaaattttaa aaggagaacg tggtgtacca atcatgtcga atattcgaga
    22321 acagctcgct cgtgcagatg atgcaacttt gcaagcgaca tttgattatt tagaaatgat
    22381 ttccgatatg gacaacggcg aagtgaatgt ctttgatagt gctttgaatc cggaaattca
    22441 agaccaatac aatcttttag ttgaaaaagt catttatgaa gaagtaacag cagaaggaag
    22501 gcatgaaaaa agaaaaacta aaacggttct tggatcaaga caatgtggtc ggttacgtat
    22561 ttgcttctcc attcatccta ggatttctat tgataacagt gataccgatg atcttgtcac
    22621 tttattattc gttcaccgat tatcgtttgg gaaatccagt ctcatggatc ggcttggata
    22681 attatataaa gatgctgagt gatgcccgtt ttttgaactc catccgagtc acttttctct
    22741 atgtatttgc atctgtacct gtgaaactga tctttggtct attgattgct tatcttttaa
    22801 cgaggaatat caaaggaacg actttctttc gttctttgta ttatgtgcct agtttgatcg
    22861 gtggcagtat ttctatcgct ctagtttggc gggagttgtt ttctaaaaac gggttagtga
    22921 atattttcct ggaacgatta ggcgcacctg cgctttcctg gtttagcgat caaaaactgg
    22981 tcatgatccc tcttgttttg atgtctgctt ggcaattcgg ttcggcaatg atcatctttg
    23041 ctgcaggtat caaagagatc ccaggctcgt attatgaagc ggcagagatc gatggagcga
    23101 acaaatggca gacgttttac aagatcacgt tacctcttct ttcgccgatc attttgtata
    23161 acttggttat gcagacaatc gcggcgttta tgacttttac gcaggcattt atcattaccc
    23221 aaggtggtcc gaatgatgcc acgaactttt atgctcttta tgtgtataac caagcttttt
    23281 cttttaattc tatggggtat gctagtgcta tttcgtggat catgctgttg attatgggag
    23341 ctgtcacctt tctgattttc aaatcatcaa aaaaatgggt cgttgtgtga atgagaggag
    23401 gaagaaatga ataatacagt aacgaaatgg tttcaatacg gttttatttt gctattaggt
    23461 ctagtcatga tttatccggt cttatggatg atcgcaggtt cttttaaaac ggatcaagag
    23521 attatgagtg gttcgctgaa tttgattcca gagacttttc aatgggaaaa ttacgcaaaa
    23581 ggatgggcgg ggtttgctgg aatttcattt tttactttct ttaggaattc gtttgtcatc
    23641 tctgcagttt cgacgatagg gacagttctg agctcgactt gtattgctta cgcattatcg
    23701 agaatcgatt tcaaaggaaa aagattctgg ttcgtcgtca tgttagtaac gatgatgatt
    23761 cctgcacaag tgattttgat tccgcagttt attatttaca atcggctgaa tttggttggg
    23821 acgtatgtgc cgctgatttt accacatttt tttggacaag catttttcat ttaccagatc
    23881 atgcaattca tggtcagcat ccccaaagag ttagatgaat cagcgattat tgacggatgc
    23941 agcaagtata gtgtctttac caaaattatt tttcctttgc tgaaaccttc gatcatcacg
    24001 acgatcatca tccagtttta ttggaaatgg gacgacttca tgggaccatt gatttattta
    24061 aacaagccga gatcttacac ggtatcgatt gccatcaaat tatttgcaga cgctggatct
    24121 agtacgactt acgcgagtat gtttgcgatg tctacgttgt cgctgctacc ggtttttctg
    24181 attttcttat tcttcaataa atatcttgtc caggggatca gtacaagcgg tctgaagggc
    24241 tgaatatgaa aaagccaata gaagagatgt ttctttacaa gctcaactac tatgtctgtc
    24301 ggattttttt aaccagtctt atcagtaatg ctcttttcgc tttagttcta ggatgtttgt
    24361 tcactttagg attagtgctg tctgctcgtg ttgtctatct gatttatttt ttgatgatgt
    24421 atccttctta ttatctatct gtccattact tggcagaaaa aaaggtatgt tggaggaaag
    24481 ggaaaatcaa acggatgtgt ttcatcatta cgttgtttca gctgtttgtc attatttttg
    24541 cagttgattg gtatttatta gtgggaaaga ttggagcttg gtatctgatt ccttttctat
    24601 tgtgtctgtt gctttttgta tcccatagtt tttttagtac gctaattgat atctgcaaag
    24661 aagataaagc agatagtctt gtttatttga tcaaaaggaa tatctggctg ttgtcaaaaa
    24721 acgtcaaggg gtttctgtgg tatttatgcc ttttgtatgg actgttctgg tgtatacagc
    24781 ctgatttttt actcactgca ttactgtatc cagggctgcc atttgtcaca gtattttgct
    24841 taaaagaaaa ggagaatgac tttaaatgaa gaaaaaatat gtacaagtcg gacttggtgg
    24901 gagagcgcgt tttttttatg aggcaattgc ctctacgtat catcaaactt gtgaactagt
    24961 cggtttttgt gatgtatctc aaacaaggat ggattatgca aatcggatac ttgtgaacaa
    25021 gtatggacag aaaaaagtaa agacatgtat gccatcacaa tttgagcaac tactggatga
    25081 cgtacggccg gctgaagtca ttattacgtc agtagatcga acgcatcatg attatatcat
    25141 tcgtgcaatg aaaaaaggat atgatgttat ttgcgaaaaa cctctgacta tcgatgagga
    25201 aaaagctcaa gaaataataa cggtccaaaa acagacggga agaagcctgc gagtcacttt
    25261 taattatcgt tatgcaccgt atcacacgaa gataaaggaa ctgatagaaa gtgatgtgat
    25321 tggtgatgtg ttttctatcc acttcgaatg gctgttaaat acgcaacacg gggcagatta
    25381 tttcagaaga tggcatcgaa ataagcacaa tagcggcggg cttcttgttc ataaatctac
    25441 ccatcatttt gatctagtga atttttggtt gaacagccgt ccccaaaagg tatttgcatt
    25501 cggtggatta aacttttatg ggatgcataa tgcggagaaa cgcggagtag aaatatttta
    25561 cagtcgatgc tatggaagcg aactagctaa agctgatcca tttgctatag acctgtcagc
    25621 caatgaggaa ttgcgagaaa tgtatttaga agcagaaaaa gacagtggat atattcgtga
    25681 ccgcagtgta ttttccgatg atatctctat tgaagataca ctaggcgtaa cggtcaaata
    25741 tgaaactggt gcgattttga cgtattcgct aaacgcgtat atgccgtggg aaggattcaa
    25801 tgtttctttc aatggcagca aaggacggat cgaaatgaaa gtgatcgaaa agtcttacat
    25861 caatgcaggt ggagataaaa aggatgaagg agcagccgtt cttcagtcca tccatgtcta
    25921 tccgatgttc ggtgagccat atgaggtaat cgtcgaacaa gaagcgggcg gacatggcgg
    25981 aggagatccg cgtttattag atgatctatt tggtgaagaa aaagaagaag atccgtggaa
    26041 tcgggcagca actcacgtag atggtatttt gtcgattttg acaggaatcg cagcaaatca
    26101 ttctattgct tcaggaaaag cagtagtgat tgacgagctt gtcagcttaa aagaataaga
    26161 atagatttac ggaaattact tcccgtagtt tataaaatct tgccttattg ccgataggct
    26221 gtcttttgaa cactatttat tcggaaaaag gggctatgac atgctttttg tcatgccttc
    26281 tttttatgtg ggctgctttt ttagatagga gaaaataaaa ataactattt aaacccgaac
    26341 aaaaaggaat aatgcattaa aaatcattag attttacaaa aaaataagta aaccacgttg
    26401 tcagtttcag aaagtagtgt tagactgttt tcaatatttg aaacaacatt ttttattcaa
    26461 atatggcaaa ctgccttcac gtgcaaaaca gttgcaagaa aatgaattta cactattatt
    26521 agttttacat acctacttat atatcgtcat aatatggatg acagtttcta gccagtaccg
    26581 taaatgctgg actataagta aaagattggc tatttcaccc ttcttttctt atgataaagt
    26641 ggagggtgtt tttctttaac agaaacatag taggtttttg cacaaaagcg gttatttcgg
    26701 tattaataaa tgataaagga gcaaataaag tgaaagaatt agttgaacgt attcaaaaag
    26761 acggacgtat attaggcgag ggcgtattaa aagtcgacag cttcgtcact caccaagtag
    26821 accctgtatt gatggaacaa atgggaaaac ggtttgcaga agtatttaaa gaacaaggaa
    26881 tcacgaaagt tgtgaccatt gaagcttctg ggatcgcacc agctttattt gctgcaagag
    26941 aattagatgt tccaatgatc ttcgctagaa aagcaaaaag tttgacgatg gatgaagaat
    27001 tattgacagc ttctgtttat tcttttacga aacaagtgac aagtacgatt tctatttctc
    27061 gtaaattttt atcttcagaa gacaaagtat tgattatcga cgatttcttg gcaaacggac
    27121 aagcggcaaa aggattgatc gaattatgcc agcaagctgg agcgcaagta gaaggaatcg
    27181 ggatcgtcat cgaaaagtct ttccaagatg gacgtcagtt acttgaagaa atgggattga
    27241 gagtcgtttc tcttgcgcgt attgcttcct tgacgaatgg acaagttgag tttcttgagg
    27301 aggacgcata atcgtggaaa aagaaacaca aaatggaaaa gctgcggttt tagggctgca
    27361 gcatctgcta gcgatgtatg caggagcagt agcggtgcct cttttgatcg gtacaggact
    27421 aggctttaat caagaacaga tgacttattt gatttcaatt gatatcttta tgtgtggatt
    27481 agcgacatta ttgcaattga cagttaaccg atttttcgga attggcttgc ctgtcgtttt
    27541 aggatgtgcg atccaagcag tagctccact gatcttgatc ggaaccaatg aaggagtagg
    27601 agctatctac ggatctatta tcgcttctgg gatctttgtt gtgttagttt ctggtgtctt
    27661 ttctaaaatt aaaaaactat tccctcctat agtaacagga acggttatca ccgttattgg
    27721 tttgactttg attcctgtag caatcgagaa aatgggtgga ggtaatgcaa cagcagctga
    27781 ttttggtgac aaaacaaatc tgctacttgc atttgtgacg attctcctaa ttgtaggcgt
    27841 gcaaatgctg gcaaaagggt tcattcgttc aatcgcggta ttgattggtt tagtcggagg
    27901 aagcatctta gctgctttca tgggaatgat tgatctgagc gcgattggag atgcgccgtt
    27961 gttccatgtt ccacaaccct tttattttgg taaaccaact ttcgacgtat ggtcgatttt
    28021 actaatgatc atcatttcca tggtcagcat ggtagaatca acaggtgtat attttgcact
    28081 tggtgacatt actgggaaaa atgttggaga agaagaattg aaaaagggat atcgtgcaga
    28141 aggacttgcc gttatcttag gcggtatttt caatacattc ccttatactg gcttttctca
    28201 aaatgtcgga ttggttcaac tttcgggaat caaaacaaga aaacccatct atttctctgc
    28261 attcttcttg attgttttag gattattccc taaaatcggt gcgttagcac aaatcatccc
    28321 tgagccggta cttggtggag gaatgttagt catgttcggg atggtggcag tccaaggaat
    28381 ccgtatgctg ttggaagttg atttcagcaa tgataaaaat ctattgattg ttgctgtttc
    28441 tgttggattt gggctgggtt tcaatgttac gccgacatta ttcagtcaaa tgccgcaaac
    28501 attgcaaatg ttcactggaa atggcatcgt aatgagtagc attacggcta tcatattgaa
    28561 tttgatcttt aatggattga aaaaagatga agaaatattg gaagaagcct tcaagaagta
    28621 atagagcaaa agaaaggttg tgaaatcagc gttttgacct gattgttaga gaaacaaacg
    28681 gaaaaatagt tcctcgtatt ttgccgtttg tttgagctaa taccgagggt cagctgattg
    28741 aacaccgttt atccataaaa aggaatgtga cctaactttt gtcacattcc tttttttcat
    28801 ctttaataaa cacgaacaat aaaatgtttt tctttgttaa cattcgaaat ttctttgaca
    28861 tcaaaaaaag tgattgttaa aatatgaatg tagaaaaact gggaggaaaa acaatgacag
    28921 caaaaatctc tgtgattatg gggagtactt cagattggga aacaatgaaa gaagcatgtc
    28981 aaatattgga tgaattcggt gtgtcttatg aaaaaaaggt cgtttctgct catcgaaccc
    29041 cagatttgat gtttgaatat gcacaaaacg ctcgaaaaaa aggaatcaaa atcattattg
    29101 caggagcagg gggagccgcg catctgccgg gtatggtcgc atcaaaaaca actttgccgg
    29161 ttatcggtgt accggtccaa tcacgaacat taaatggttt ggattcgctt ctttcgattg
    29221 tccaaatgcc tggaggagta cctgtagcta ctacagcaat cggaaaagcg ggagcaatta
    29281 atgctggact acttgcgata cagatgcttt ccatgtatga tttagaattg gaggctaaat
    29341 tggctgaaag acgttcaatg ttggctaaaa ctgttataga aagtagtgat caacttggtt
    29401 aaacctttgt tgcctggttc aacgatcggg atcgtcggtg gcggtcaatt aggtcgtatg
    29461 atggcgatta gtgcaaaaga aatgggcttt aaagtaggtg tgctggatcc tgtgagcgat
    29521 tgtccagccg cacaagttgc agattggcac attgaaagtg tctatgatga cacctttgcg
    29581 ctgaaagagc ttgccagaag aacagatgtc attacctatg aattcgaaaa cgtcagtgta
    29641 gaggcgttaa atgcgatttt acccatgtca tttattcctc aagggacaga cttgcttgcg
    29701 attacgcaag accgcctgtt agaaaaatct tttttggaaa cgaataacat cgtgattgct
    29761 ccttatgcaa cgatcgtcag tccgacggat atccaagatg cgattgacgg gatcggctat
    29821 ccttgtgttt tgaaaaccac tcgaggcggc tatgatggca aggggcagta tgttttgaaa
    29881 agtcgggcgg atctagctcc tgccatggac cttttaagag aaggaacctg tgttttagaa
    29941 gcatggatcc cgtttgaaaa agagatttcg atcatggtgg caggaaatgg tcaaggagat
    30001 ttcacgactt ttccggtcgt ggaaaacatc caccacaaca atattcttca tgaaacgatt
    30061 gctccagccg cgattgatca ggatgtgatc gaggaggctg agcgtattgc ccgtgtgatt
    30121 gcagaagcag tgagcctttc aggtgtttta ggggtagaaa tgtttttaac aaaaacaggc
    30181 gggctatatg tgaatgaatt agctcctcgt cctcacaatt ctggacatta ttctattgaa
    30241 gcttgttcta tgagtcagtt cgatgcccat attcgaggta tttgtggttg gccattagga
    30301 gaaaaagtac gtttgataag caatgcagta atggtcaatg ttttgggaga acaactgctg
    30361 gaaagctatc gcttgattga gaataagcca gactggcaat tccatttcta tggaaaaaaa
    30421 caagcaaaaa aaggtcgcaa gatgggacat atcactatct tgacagaaga catccagcaa
    30481 acgctggaag aaatcaaaca aacgaatatt tgggattaga ggagaaaaaa catgttagaa
    30541 cgttatacac gccctgaaat ggggaatatc tggacggaag agaacaaata ccgtgcgtgg
    30601 ctcgaagtcg agattttagc agatgaagct tgggcagaac ttggtgaaat cccgcaagaa
    30661 gatgtgaaaa aaattcgtga gcatgcatca tttgatatcg atcggatctt ggaaatcgaa
    30721 aaattaacac gccatgatgt cgttgcgttt acacgagcgg tttctgaaac attgggagaa
    30781 gaaagaaaat gggtccatta tggtctaaca agtacggatg tcgtagatac tgcttatggc
    30841 tatctcatca agcaagcaaa tgatatttta cgggaagact tgaaacgttt tacggaaatc
    30901 attggcgaaa aagcaaaaga acacaaatat acagtgatga tgggtcggac acacggtgtc
    30961 catgctgaac caactacttt tggattgaaa cttgccacat ggtattcaga aatgaaacgg
    31021 aacatcgaac gatttgaaca tgcggcaaaa ggagtagaag caggaaaaat ctcaggagca
    31081 gtgggaacgt tcgcaaatat cccgccattt attgaagagt atgtatgtaa acaattgggg
    31141 atccgtccac aagaaatctc tactcaagtt ctccctcggg atctacatgc tgaatatttc
    31201 tcagcgatgg cgcttattgc tacaagtatt gaacgttttg caacagagat acgcggattg
    31261 caaaaatccg aaacacgtga agtagaagaa ttttttgcga aaggtcaaaa aggttcttct
    31321 gctatgccgc acaaaaggaa tccgatcggt tcagaaaata tggctggtct agccagagtg
    31381 gtgcgcggac acatggtcac tgcctatgaa aatgtcacat tatggcatga aagagatatt
    31441 tctcattctt cagcagaacg aatcatcttg cctgatacca cgattttatt gaattacatg
    31501 ttaaatcgtt ttggcaatat cgttaaaaat ctaacggtct ttcctgaaaa catgaagcgg
    31561 aatatgtctg caacatttgg tttgatttat agccagcgcg ttatgctgaa attgatcgat
    31621 aagggaatga caagagaaga agcttatgat cttgttcagc caaaaactgc gtacgcttgg
    31681 gacaaccaag tgcagttccg cccgctgtta gaagaagatc caaccatcac atcggtatta
    31741 tctaaagaag aaatagacga tgcctttgat tatcatcatc atttgaaaaa cgttgacgtg
    31801 atattcaaac gagtaggttt ggaataaaga agagtgagtt gcaaaattca attaaaatag
    31861 aatagcaaaa ataaccaaaa aagaagcaga tccgttagcg gttctgcttc ttttagttta
    31921 ttatattttc ttatcaataa taggaacctg attttaaaag cggctacctc tttaccgtgc
    31981 ctctttacat acaaaaaata gaggaattaa gcgtaaatgg cttggctatt ataactaaaa
    32041 tgctggttat ggagatgttg cagcaagttt tcgagaaaag cgtattccat ctcttgtcca
    32101 ttgaataaga tctggatacg ataatgattt ttgaaacgct tctgttcatg aatctgactg
    32161 attgaaagtt cgcctttgtg ttctgtctga taaagaaaag tccttttacg gcattcagca
    32221 cagcgcttgg ttgctcggcg gatttttcgg aatgtataaa aactagtatc tttattgcat
    32281 aaataaacgt ctttgatcga cagcaaatca ttatatagac aggaacgttc ttcagctgtt
    32341 aattctgata aaaaaagctg accttgtttt attgcatctt ccatcggcaa gtgcctcctc
    32401 gaacccatta tccaaatttt tagttaattc taatatacca tcattaagta taaatgtaaa
    32461 cgcttgttga tggtataaga ttttcgtttc tgataaaaca cgaacattaa aagaatctaa
    32521 tgccttaaat gtacatattt ttgttgattt ataaaaagat gattgctaaa atttaaagtg
    32581 aaaaaacgaa caaaaaacga tgtatttgct tttaatgtaa ataaaaagga gagacgtaca
    32641 tggagaagaa agaacttttg tatgaaggaa aagccaaaaa aatatatctt acttctgatg
    32701 agaacttgat ttggatagaa tatttagatc aagcaactgc gttgaatggc gcgaggaaag
    32761 accagataag tggaaaagga gaattgaata atcaaatcac aagtgcaatt ttccgttatc
    32821 tgactcaaag aggaataaaa aatcatttta ttgaacaatt gtctaaaacc gaacaattag
    32881 caaaaaaagt acaaattatc cctctggaag tggttatccg aaatattgca gcaggaagtt
    32941 tctcaaaacg tctaggtgtt cccgaaggta aagaattcat caaaccaatc attgaattct
    33001 actataagga tgaccagttg gatgatccat ttatcaatga agagcatatc tatttcttac
    33061 aacttgcaaa accggaagaa atcagtgagc tgaaagccca agcgttggct attaatgaaa
    33121 ttttgaccga attatttgct aaggtcggtt tggttttagt agatttcaag ctagaatttg
    33181 gccggtcgaa tggacaaatt ttgcttgcgg atgaagtaac accggatact tgccgattat
    33241 gggatgaacg tacgaatgaa catatggaca aagacgttta ccgcagagat ctaggtgatt
    33301 tgatcccagt ctatcaagaa gtatataacc gtctatcgga actaactgaa gaaaaggagc
    33361 gagtatgatg tatttcgtaa aagtatatgt aacctataaa gattcagtgt tagatcctca
    33421 aggggaggca gtaaaaaaag cagtccatcg tatgggatat gaaacaatcg aagatatccg
    33481 aataggaaaa tactttgaga tacaacttgc tgatgcagaa gaaccaatcg atacagtgat
    33541 tgaaacgatt tgtgacaagc tgcttgcaaa tgtaaatatg gaaacatacc gatatgaaat
    33601 caaaaaaatg gaggaagtat aacatgaaat ttgcggtcat cgtttttcca ggttcaaatt
    33661 gtgacgcaga tatgttatgg gcagttcgcg agatcatggg cgctgatgcc gaatttgtac
    33721 ggcatgatga gtcctcttta gaaggatttg atggggtttt actgccggga ggattctcct
    33781 atggtgacta tttgagatgc ggtgcgattg ctcggttttc tacaatcatg gaagaagtga
    33841 ttcgttttgc tgaagaaggg aaaccagtat tcggtacatg caatggattc cagatattga
    33901 cagaagcagg acttttgcca ggtgtactga gacgaaatga gtctttgcat tttatttgca
    33961 aaactgtacc attgaaagtc aaccccaaga cggcctttac ttcagagtat gaagccgacg
    34021 aaataatcta tcttccagtt gctcatgggg aaggaaatta tttctgtgac gaagaaacac
    34081 ttcaagaact aaaaaaaaat aatcagattg cctttactta taatgacaac atcaacggca
    34141 gcgtggaaaa cattgcaggg attacgaaca aagcagggaa tgtgttagga atgatgcctc
    34201 atccagaacg tgcagtagaa gacttgctag gatcagaaga tgggaaacgg ttctttgctt
    34261 ccattttaaa aaacttcgga aaggtgaccg tatagatgaa aacattgaaa gaaccgacag
    34321 ctgaagagat caagaaaaaa agaatctatg ccgaatgggg attgactgat gaagaatatc
    34381 gaatgatcga agaagatatc ttaggaagaa tgcctaatta tacggaaact ggactgtttt
    34441 ctgttatgtg gagcgaacat tgttcctata aaaattctaa accggttcta cgcaaatttc
    34501 ctactagtgg acctcatgtg ttgcaaggcc ctggagaagg cgcaggcatc gtcgacatcg
    34561 gtgatggact agcagttgtc ttcaaagcag aaagtcacaa tcatccttca gccgtggagc
    34621 cttatgaagg agctgcgaca ggagttggcg gaatcatcag agatattttc agcatggggg
    34681 ctcgtccaat tgcgttgcta gattcattga ggttcggcga attagataat ccaagaacga
    34741 aatatttact agaagaagta gttgcaggta tcggcggata cggcaattgt atcggcatac
    34801 cgactgtagg cggcgaaatt gcatttgatc cttgttatga aggaaaccca ttagtcaatg
    34861 caatgtgcgt aggtttgatt gaacacaaac atatccagaa agggcaagcc gctggcgtcg
    34921 gtaattcgat catgtatgta ggtgcaaaaa caggtcgcga cggtatccac ggagcaactt
    34981 ttgcctcaga agaatttaat caagaagaag aacagcaacg ttctgctgta caagtaggcg
    35041 atccttttat ggaaaagctg ctgcttgaag cgtgtttgga attaatcttg catcattcgg
    35101 attctctaat aggtatacag gatatgggag cagcaggact tgtttcatct agtgcggaaa
    35161 tggcttcaaa agctggctca ggacttatat tgacattaga tgaagtccca caaagagaaa
    35221 ctgggatgac accttatgaa atgatgttat cggaatcaca agaacggatg ttgatttgtg
    35281 ttaaatcagg agaggaagaa aaaatccaag aactatttca gaagtatgat ctagatgcag
    35341 tcactatcgg taaagtaaca gacgacgggc aatatcggct atatcatcaa ggagaagaag
    35401 tagcaaatct gccagtcgat gcattagctg aagatgcacc agtttatcat aaagaaatga
    35461 aagaaccagc tcgaatcgta gaatttcagc agttagctcc ttatcagcca gttatcgaag
    35521 aacctggaca agtattgctt gatctgctgc agcaaccgac gattgcttca aaacgttcaa
    35581 tctacgaaac ttatgattca caagtacaaa ccaatacagt tgtccgtcca ggaagcgatg
    35641 ctgctgtgat gagggttaga gggacgaata aagcgttagc tatgaccaca gactgcaacg
    35701 cgcgctatct gtatttggat ccaaaaatcg gaggacaaat agcagtggca gaagctgcta
    35761 gaaatattat tgctagcggt ggtaagccct tggcgatcac cgactgcctg aactatggtt
    35821 ctcctgataa accagaagtt ttttgggaat tatcgacttc agctgacgga attgctgctg
    35881 cgtgtgaaac gttaggaact cctgttattt caggaaatgt ctctttatat aatgaaacgg
    35941 atggtcaagc aatctatcca acaccaatga ttggtatggt cggattgatt gaggatcata
    36001 aacatatcac tactcaggaa ttcaaaaaat caggggatct aatctatata ttaggtaaga
    36061 cgtttgcaga ttttgatggc agtgaattac aaaagatgca gcttggaaga atcgaaggtg
    36121 tgatccgaaa ctttgatcta tccattgaaa agagaaacca agagttagta ttgacagcca
    36181 tccaaaacgg attgattgaa agtgctcatg actgttcaga aggtggcttg gcaattgcac
    36241 tggcagaatc agctttcaaa catcagctag ggatatctgt acagtttgaa ttgtcctctg
    36301 ctcaattatt tgcagaaact caatcaagat ttgtgttgac agtcgcaccg gaaaataaga
    36361 cacgattcga agaaatgatg ggagatgctg ctgttttagc aggtaaggtg acggatgaag
    36421 cgataatcga aatttcagca actgacggtc agatcaaaat agaaactgca gtagcaagaa
    36481 agtgctggga ggatgcaatc gtatgtctta tgaagtgaag agtttaaatg aagaatgcgg
    36541 gatcttcggt gtttggggtc atccagacgc cgctagtgtg gcatacttcg ggctccatag
    36601 tcttcagcat cgaggacaag aaggtgcagg tatcgtgtcc aatgatcgag gaaagcttaa
    36661 ggggcatcga gatttggggc tgctttctga ggtattcaaa gatcaaagaa aattgcagca
    36721 gttaacagga gaagctgcaa tcggacatgt acgttatgca acagcaggca atggaagtgt
    36781 ggacaatatt cagccttttc tattcaaatt tcatgatcag tcgatcggct tagcgcataa
    36841 tggtaacttg atcaatgctc gttcccttag aaaagagtta gaggaaaatg gggcgatctt
    36901 ccattctaac tccgatacgg agatattgat gcatttgatc cgacgaagtg aagaaacaga
    36961 ttttttaaat aaattaaaaa gtgctttgaa tgaagtcaaa ggtggctttg cctatttatt
    37021 aatgacagaa acggcaatga ttgccgcttt ggacccaaat ggttttcgtc cattagcaat
    37081 cggacaaatg aaaaacggag catacgtgat tgcctcggaa acttgtgcat tagaagttat
    37141 tggggcacgt tttataagag atgtccagcc tggtgagata gtgatcatca atgacgaagg
    37201 aatccagatt gattgcttta cgaaggacac gcagctttct atttgttcga tggaatatat
    37261 ctattttgcc agaccagatt caaacatcgc agggatcaat gtgcatactg cacggaaaaa
    37321 tatgggacgg aatttagcga aagaatcgcc agtaaaagca gatatggtcg ttggcgtacc
    37381 taattcttct ttgtcagccg caagcggtta tgcggaagct agtggaatcc cttatgaaat
    37441 cggattagta aaaaatcaat acattgcccg aacatttatc cagccaacgc ctgagctaag
    37501 agagcaaggt gttcgaatga aattatctgc tgtacgagga gtagtggaag gtaaaaaggt
    37561 gattatggtc gatgattcaa ttgttcgggg tacgacaagc agacggatcg tccatctttt
    37621 gaaagaagca ggggcaaaag aagtccatgt gaggattgct tctcctcctt tgaaatatcc
    37681 atgtttttac gggattgata ttcaaacgcg taaagaactc attgcggcaa atcacacgat
    37741 tgatgaaatc aaagaatgta tcggtgcaga ttctttaggt tttttaagtg aagagggtct
    37801 gatcgaatcg atcggtctgg aaaaagatgc tccgtattca ggattgtgta tggcttattt
    37861 caacggagat tatccgacgc cgttatatga ctatgaagaa aaatatcagg aatcactaaa
    37921 agaaaaagta tcgttctttt aaaagcagac caagaaggga gtatatgaat tcaatggcaa
    37981 atgcatacgc aaaagctggc gtagatgtcg aagctggata tgaagtagta gaacggatca
    38041 agaagcatgt caaaaaaacg gaacgtttag gtatgatggg cgctatagga ggattcggcg
    38101 gttgttttga tttatcggtc tttcaattaa aagagccagt ccttgtctcg gggaccgatg
    38161 gcgtggggac gaaattagca gtggcaatcg aagcagacaa acacgataca atcgggatcg
    38221 actgcgtagc gatgtgtgtc aatgatatct tagcacaagg cgcagagccg ttatatttct
    38281 tagactactt agcaacgggg aaaaatgatc cgttacgttt ggaaaatgta gtaaaaggtg
    38341 tagcagatgg atgtgttcaa ggaaatatgg cgctgattgg cggagaaaca gctgaaatgc
    38401 ctggtatgta ctcaggtaat gattatgatc tagccggatt tgctgtaggg atagctgaaa
    38461 aatctcaatt attgacagga gaaacgatca acccaggaga tgtgttgctt ggaatagcat
    38521 ctagcgggat tcattcaaat gggtactctc ttgttcgcaa aattttcttt gagcagcagc
    38581 agttttcttt atcatcaaaa ctgccagaat tgaaaaaaac acttggcgaa gaattgcttg
    38641 agccaacgaa aatctatgtt tcttccttgc ttccgctgat caaagaaaag aaaataaaag
    38701 gagctgcaca tatcactggt ggagggtttg tagaaaatat cccgcgaatg ttaccagaat
    38761 ctatggctgc acaaatcaaa cttggctcat ggccgattct tccaatattc ggctgtttac
    38821 aaaaatatgg tgaaattcct gagatggaga tgtatgaaat cttcaatatg ggcatcggta
    38881 tggtcctggc agtcgaagaa tcgaaagtaa aagaaatagt acacgaaatt gagcaagctg
    38941 gcgaaaaagt ttatgaaatc gggaaagtga tcgccaaaga aaaacatcgt gtggtgttta
    39001 taggagaaga acgatgagaa tcgctgtttt tgcttctgga aacggaagca actttcaagc
    39061 gctagccgat tatctatcga aaaaaggatt ggaatcttcg attgactggt tgttttgtga
    39121 tcagcctgaa gcatatgttt tgaaacgtgc aactgcgttg agtgtaccgg cagattgttt
    39181 ttcacctaaa gaatttgatt caaaaaaaga gtatgaagaa gcaattttgc ataaattgaa
    39241 agaaaaaaag atcgatttga tcgtattagc tggatatatg cgaatcattg gaccggtatt
    39301 gctgaaaaat tatgataaaa gaatcatcaa tatccatccg tctcttttac ctgccttccc
    39361 tggtttgcat ggcatacgtg atgcctttga agctggagta aaagaaacgg gtgtcacaat
    39421 tcattatatc gaccaaggtg tagatactgg accaatcatc cggcaggaaa aagtgagaat
    39481 tgaacaggag gacacgttcg attctttaga agaaaaaatc catagagtag agcatcgaat
    39541 ctatccagaa gtgattagtg aaattattga aaatggaggc tatctgaaat gacaagagca
    39601 ttgatcagtg tatcagataa aactgggatt gtagcattcg cccaaggttt gattgaacaa
    39661 ggagtagaaa tcatttcaac aggaggaaca aagcatgcgt tggataaagc tgggatcaaa
    39721 acgctttcga tcgaagaagt gacagggttt cctgaaatga tggatggccg tgtcaaaaca
    39781 cttcatccta aaatccatgg tggtctttta ggaagacgcg atctccctag ccatatggag
    39841 gcaatggaaa cggaaaatat ccaaccaatc gactttgttt gtgtcaattt atatcctttc
    39901 aaagaaacga ttatgaaacc agatgtcgaa atagcagaag ctattgaaaa cattgatatc
    39961 ggtggtccta gtatgctccg tagtgcagca aaaaaccatg aatttgtcac ctctgttgtt
    40021 gatccatctg actatgaaga agtactttca gaattacgtc agtatgggca tacttcactt
    40081 tctttcagac aaaaactagc ggcaaaagtc tttcgtcata ctgcggcata tgatgcactg
    40141 atcgctcaat acttaacgga ttgggtggaa gaaaaagaac cggaaaaatt gacacttacg
    40201 tatgaacgaa aacaaacgct tcgttacggt gaaaatagcc atcaagccgc tgccttttat
    40261 cagtccgctc ttctggaaaa atattcgatc gcagcagcaa aacaactcca tgggaaagaa
    40321 ctttcttata ataatatccg agatgcagat gctgcgctac ggatcatgaa ggaatatgac
    40381 gagccaacgg tagtcgcctt gaaacacatg aatccttgtg gtattgggac agcatctacg
    40441 attttagaag cgtgggaagc agcgtatgaa gcagatcctg tttcaatctt tggtgggatc
    40501 atcgttttga atcgagaagt agatctgcca acagcagaac agatgcataa gctgtttcta
    40561 gaaatcatca ttgccccttc ttatacacaa gaagctttgg aattactgca aaaaaagaaa
    40621 aacatccgat tactgacagt agactttgcg caggaagaaa atcaaaatcg agaagaaacg
    40681 gtctcagtct taggtggact tcttgtacaa gatcaagatc tggcaataga aaatgaagaa
    40741 gagtggaaag tcgtgacaaa acgtaagccg actccagcag aagcaaaagc attagcgttt
    40801 gcttggagag ctgtcaaaca tgtgaaatct aatgcgatcg tactggcaaa cgaacgtcag
    40861 acagtaggaa tcggagccgg gcagatgaat cgagtcggtt cagtaaaaat tgcgattgaa
    40921 caagcgcaag agaaaatgga aggtgcagtt ttagctagcg atgcttattt tccaatggat
    40981 gattcggttg aatatgcagc caaacatggt ataaaagcaa tcattcagcc aggaggaagc
    41041 atcaaagacc aagattctat tgatatggcg aataaatacg atctagcaat ggtctttact
    41101 gatgttcggc attttagaca ttagagagga gtcggataaa tgaacatttt ggtgatcggt
    41161 gcaggtggtc gtgaacatgc aataagtaaa aaacttttag ccagtccgaa ggtggatacg
    41221 gtgtattgtg cgccaggaaa tccggggatg acaaaagatg gtatcaattg tgtcgctatc
    41281 agtgagcgag atcattcagc gttaatccat tttgttaaga aagaggctat tgattggagt
    41341 atcgttggtc ctgaagttcc tttgttaaat gggatcgtcg atgattttat ggcggaagga
    41401 ttgaaaatct tcggtccgac aaaagcagca gctctgatcg aaggttcgaa aagttttgct
    41461 aaacaattaa tggatactta tcaaatcccg acagctaaat ccagaagttt tgaatgttac
    41521 gaaaaagcag cagcgtatat caaagaaaat ggtgcgccaa tcgtagtgaa agcggacggc
    41581 ttagcagctg ggaaaggtgt agtcgtggct accacagtga aggaagcgct ggatgcggca
    41641 gaacaaatgt tgaaccagca tcgatttggg gatagtagca aaaaaatcgt cgtagaagag
    41701 tttttggcag gagaagaatt ctcccttttg gcgtttgtcc gtaataccaa ggtgtatccg
    41761 atggtcattt cgcaagatca taagcgggca tttgataacg ataaagggcc aaatacaggc
    41821 ggaatgggtg cttattcacc agtaccgcaa atttcaaaaa agatgattga aaaagctgtg
    41881 gaagaagttt taaaaccagc ggctttggga atgaaagaac taggacgctc tttcacgggt
    41941 gttttatatg cgggtttgat tgctacgaag gaaggaccaa aagtgatcga attcaatgcg
    42001 cgtttcggtg atcctgaaac gcaagtcgtt ctttctcggt tgaagagtga ctttgcgcaa
    42061 gtaatcgatg atttattaga gaatcgtaca gtagagttaa agtggcagca agaaggatac
    42121 aatgtaggcg ttgtggttgc agctgccggt taccccgaag aatatgagac aggaatggtt
    42181 ctaccagatt ttctggaaga agagctttct gtttactatg ctggagtttc ctcaaataac
    42241 ggcaaactag ttggagcagg aggacgtctt tatttagtag aagcatacgg tgagacgatc
    42301 aaagaagcac agaaaaaaat ttacaccgcg ttaatggata aaacgacaga tggaacattt
    42361 tatcgtacag atattggaga aaaagctgcc aatagcgaac tgatcgtgaa gtaaatcagc
    42421 tgcttccatt tataaaaaat acaacaaaca taatcggaag cgccgatttt agtgaagcct
    42481 tttcataaag agtgtggtac acttttgttg tgtatataaa taaggaggag ttcagatgaa
    42541 aaaaattcaa ttaggtactt ctgatcagaa agcagctgca gtgatcttag gctgtatgcg
    42601 tatcaacaat gcggaagatc cagtaaaagt gattgagaca gcttatgaaa acggcattga
    42661 tttttttgac catgcagata tttatggaaa cggtgaatgt gaagaaatct ttgcagatgc
    42721 tctagcaaaa acatcaatca aacgagaaga tctttttatt caaacaaaat gtggaatcgt
    42781 tccaggtgtg atgtttgatt tttctaaaaa acatatcatt gaatctgtag aaaaaagttt
    42841 gacccgtctg aaaatggatt atgtggatgc tttacttctt catcgtccag atacattggt
    42901 agaaccagag gaagtagctg aagcgtttga tgaattggaa cggtcaggaa aagttcgtta
    42961 ttttggcgtc agcaatcaaa aaccaatgca aatcgaattg ctgaaaaaat atgttcgtca
    43021 accattagta gctaatcagc tgcaatttgg cttgaaacat actggaatga tcgatcaagg
    43081 gattcatgtc aatatgacag atgaagcaag tatcgatcat gacggtagtg tacttgatta
    43141 ttcccgattg catgatatga cgatccaagc atggtcacca taccagtatg gattttttga
    43201 aggtgtattt atcgggaacg aaaaattccc tgaattaaac caaacattag aagaactagc
    43261 agctaaatat caaacgacac caactggctt ggcttctgct tggattttac gccatccagc
    43321 caatatgcaa gtgatcgccg gtacgatgaa cgtagggcga atcaaggaaa tcgctcaagc
    43381 ttcagaaatt aatatgtctc gggaagattg gtacgaattg taccgtgctg ctggaaacat
    43441 tttaccttaa agattgtcat aagcaggtca aaagggctat aaattaaaaa gacagaataa
    43501 cttgaaagct gttttcagct tcgagatatt ctgtcttttt ttattgcaaa tggaaagagt
    43561 atcattgtac ggatttcgtt tattagttat ttttgttttt aaggaaaagc tgtgtccgat
    43621 attcttttgg agaaatccct tgttttttct taaataaata actgaaatag ttagggtcat
    43681 tataaccaat ttcaaaagca atatctgaaa tacgatagtc tgattgctgc aataattttt
    43741 ttgctaattc gattctttgt tctgtcagat aatcgatgaa tgtttggtta aacgattgag
    43801 aaaaaatcgt actgaaatga gagggactta atgcaacttg ttgagcgacc caagttaagg
    43861 acatatctgg atcggtaaaa ttttctttga taaaatccaa tgcatggttc aaaattggct
    43921 gatatttgtt ccttgaaggc tgtgagggca gagcagttaa gaaagaaatg aacatataaa
    43981 ctgtcgaacg gtaaagcgag tcgtcggcgc tgattgctaa gcgttgactg gtggatgtgc
    44041 aatgaaccat ttcttgcggg attttctttt ctttcaggca gacatgctgg agtagcttag
    44101 tcaagacaaa taaacgatat aatcgtgttc gagttggtgt gccttgtggt ttcgtcaagt
    44161 gttcgatgta cttagatact tcgtttttcg ataatgaaga caaatcaagt gagaggtcaa
    44221 agtaaaagac agaatcaact ggaagtttcg cttcttgaat caaatcctca tagctgataa
    44281 tttgttcttt aagggaactg gcggtttcaa gtatcatctg cgtgatctca aaagatgcag
    44341 gtaattcgct gattctttct gcgacaggac caatggcgac agaaaaatta tttttttgtc
    44401 ccttttctaa ttcatggacc aacgtttggg ctacctgata actggtctcg atcacatttt
    44461 cttcaagaga atcaactatt aaaaatttga tgaatcgaga ggaaacagca gagaagatca
    44521 cttgttgatt ctccctaaat aaaaacttca aataatcact gagatgagcg taatcttcaa
    44581 agtctgtact gacttggttt gctgctaata atacggtcat ttttttccct gctaaagagc
    44641 gattgaaaga ttcagcaagt ttcaatgttt cactgatcgt aagtgagcct ttgaacaaac
    44701 cgttcaagaa atgatttttt ttcaattcga aaacaagatc agaagaagtg tgcgtttctt
    44761 tttgctgcaa atctaaagca gttactgctt tttctattgc ttgtttcaat tcgctacttt
    44821 taatcggttt aagcagataa tcatctgcct gtacctggat tgctcctttc atataatcga
    44881 aatcgtcgaa tccactaata aatatgagac gagtccaagg aagtagtttc cttgcttctt
    44941 ttgaaaaagt caacccgtcc atgaaaggca tttggatatc agtcagaatg atgtcgggct
    45001 gtaagtcgat aattgaagac agtgccatct caccatctcc agcttcgccg acataagtga
    45061 ttggataata agaaccaagg acagttattt gttcccgcaa gttattgcga atgaggtatt
    45121 cgtcttcgat gataaaaact ttatacatgt ttgtttcctc caattgtttt gggagataga
    45181 gggatcatca gtgtgacagt tgttccttct cggtatttgc tgtcaatcgt gataggtttt
    45241 tgatcatttc cgtaataaag aaggagacgc ttgtttacgt tgtaaagtcc gtagccgata
    45301 ctgaaatctg aatcgatccc tttggctaac tctttacgta tttctgccag tttttctgca
    45361 gggatgccaa tcccgttgtc tataactttg aattggatca tctcatcaat tttgcatgcg
    45421 gtaatacgaa taaatccttt gtgacggacg tacttcgttc catggtatac agcattttca
    45481 atcaatggct gcaagatcat ttttaaaata gaaacaggaa aaagtgtttc tggtatatcg
    45541 atcttatagt caagcatatg gccgtatctt atttttaaga tgactagata atcattgata
    45601 tggctaattt ctttttcaag aggaatccag tcttgtcctt tgcttaaaga cgtacgaaaa
    45661 aagccggaaa gtgcatgggt catagtcaga gcttcttcaa agcagtcttg ttcgatcaaa
    45721 gaaataatgg catccaaact gttataaata aaatggggag taatctgagc ttgcaataca
    45781 cgaacttcac tttgtgctaa atggtattgt ttcaaagcgt tctcccgtaa agactggttt
    45841 agatcttccg ccattttatt catgctagcg gtaagtgttt gtaattccag tgtttccggc
    45901 agggttaaac gaaagccaag gttccctgaa gaaagttgtt ctgacatctc gatcagctga
    45961 ttgatcggtt cttggacctt tctctctaaa aagcgacgat tctttttcgc atagaaaaaa
    46021 atggtactaa gaactccaag ttccactatt gttaatagaa tgagcgagcg gacaagtgtc
    46081 gtattttttt tgcttgctaa gtcgatctcc acacgaacaa aatcttgtaa gatatctgac
    46141 agcagttgag tgacagaatc tacctgattg atgatctctt cattggtatc cataaaatct
    46201 tttgccaaga tattattgat cagctcgttt tgataatctt ccagtgtatc gatcgtacgt
    46261 agcgatacat ctaacgtact aatttcttta agtgtatttg tattattttt gatctctcga
    46321 atatcattcc taagttcttc taggatattt tcctcattga actcatctac ggttacttgg
    46381 ccagaaatca agtcccacat actttccaaa actttttgat caaccttcaa agcaattgca
    46441 tctgcttttt ggatatttgc gatcgtctgt tgataaatca aaagctgttg cgaatataac
    46501 agtatgctca gtagcaaagg gataacagca aatagaagca tggctttgtt tgcttgggca
    46561 aaaatctgtt ttatgcttaa attttttgtc atgatacttc tccagtcaat aatttctggt
    46621 tgggatgtta gctatattct gctgggaaaa gacagtctcg gaaataaaaa tatctttagg
    46681 taatgttttt cctgtttgaa tatttccaaa ataacgagta acggtattgg ctacatacca
    46741 gccgacatca ggtttacact cgactacaca attgacgatt cctgagcgta gcttctcgat
    46801 catatcttct tgtccatcaa tagttacaat gatcaaatct tctccaggtt tgattgatgt
    46861 ttcttttatc gcagctaaag caccgtgagt catttcatca ctatggctgt ataatacatc
    46921 gatttcggat aaatcatttg tttctaaata tcgtttcatc tggtcttttc ctttcatccg
    46981 aataaaatca ccgacaattc ggtgaacaat agtcatcgga ggtttatggt cgatggttcc
    47041 atcttttttt agggttggtt tacgattgat tgattccaaa aatccttcgg agcgtagctt
    47101 ggtcggtgtg gagttctctg aaccagcaag ttctaggatt ctgatttcct gtttctttga
    47161 atttgtgaag tgattgctga caaataaacc cgcccgattt ccttctgctt tgaaactggg
    47221 gccaatgtgg gtgacgaaaa gctcgggatc attcgtttga atatgtctgt cgacaagtat
    47281 gacagggatc cctgctcgct ttgcttcttt taatacagag tcccacccgg attcttgcaa
    47341 aggagtaaaa atgatcatat ccactttata ggcaatgaac gtccgtatat cttggatttg
    47401 ccgttcctga ttcatgtagc catttcggta gagaacttgg taatcttctt tttcaagcgc
    47461 agtgataatt gattcagtat gctttttccg ccagctactc tccgttcctg attgcgaaaa
    47521 accaacgact agtgggtcag gtgcttcctt tgtattaggt gtacacccca tcatttggta
    47581 agcgattaca acaagggcta aaaaaaataa aacacgtttc actttaaatc actccatctg
    47641 gatttgttag tactatctta tcagataaaa gggaaaatca aagtcaaaag gaatcaatcg
    47701 aaaaaaatcc aagaaactta aaaaaatcca agacaaaaaa agtacaccta aaaaatttaa
    47761 agtaaatccc ataaaaaaat caatgttaac gctttcttta ttagctatac tcggtgtatc
    47821 aattgaaatg ggggataaaa aatgaaaaac aaatttagaa agattgttgg gactatcgct
    47881 attttgaccg caagcattct attaagtgcg tgtggcaatt caggatctgc agatgatagt
    47941 acggggtatg taggaatcgc tatgccgacc aaatcagcag aaagatggat cgctgatggg
    48001 aacaacatgg tctcggaatt agaaaaactg ggttataaaa cagatcttca atatggagaa
    48061 gataaagtag aaaatcaagt agcacaaata gaaaatatga ttacaaaagg ggtagataca
    48121 cttgtgattg cttctatcga tggttcggct ttaactgatg tgttagctaa agcaaaagaa
    48181 gcagatatca aggtgatcgc ctatgatcgt ttactgatga attcagaaaa tgtcgattat
    48241 tacgcgactt ttgataattt tggagtaggt gtatcgcaag cagcttatat tgaagagcac
    48301 ttaggattga aagaaggaaa aggaccattc acgattgaat tatttggtgg ctcgcctgac
    48361 gacaacaacg cactaatcaa ttacaacgga gtgatgtctg ttttacaacc atatatggac
    48421 aacggtcagt tggtcgtgcc atctggacag acgagtttca gtcaaatcgc tacgcttcgc
    48481 tgggatggtt ctacggcgca agcaaggatg gataatctgt taagcgcaaa ttatactgac
    48541 caaacattgg atgctgtttt gtcgccatat gatccaataa gtttaggaat catttcgtca
    48601 ttaaaaggcg tggggtatgg gagtgaaagt aaacctttac ctgtgattac tggacaagat
    48661 gcgacagttg ctggcgttaa atcgatcatt gccggggaac aaacacaaac gatctttaaa
    48721 gacacacgta tcttagcaaa aaatacgatt gaaatgatca aagcaatcag tgatggagaa
    48781 gaggttccgg tcaatgatac tgaaacttat gataatgggg tgaaaactgt ccctacgtat
    48841 ttagcaaata cagtatctgt cgataaagat aattatcaag cagagctgat cgacaccgat
    48901 tattataaag aatccgatct gaaaaattaa gaggaaccaa acaaggaact aaagagatct
    48961 aagtcagaga gaggataaga attatggcgg attgtatttt aaaaatggca gatatcacca
    49021 aaaaattttc tggaatccga gcgttagatt gcgtgaatct gatgattgaa cgtggggaaa
    49081 tccatgcatt gtgcggagaa aacggagcgg gaaaatcaac attgatgaat gtcttgagcg
    49141 gcttgtatcc atatggctcc tatgacgggg aaatctttta tgatggaaag ctgtgtcaat
    49201 ttaaagattt gaaagatagt gaagaaaaag gcatcgtcat cattcatcaa gaattagcac
    49261 taagtcctta tctttcaata aaagagaata tttttctcgg aaatgaacaa gcagaaagag
    49321 gcgtgattga ttgggaactg acagaaaaga aaacatatga actgctgaat aaagtcggtt
    49381 tgtctatcga cccgaatctt ctagtatccc agattggcgt agggcaacag caactagttg
    49441 aaatagctaa agcattatca aaatccgtcc gtctgttgat tttggatgag cctaccgctg
    49501 cattgaatga agaagaaagc gcgaatttgc tgagattgat ccgagagttg aaagagcaag
    49561 gtgtaacctc catcattatt tcacataagc tgaatgaaat cgtggatatc gcagatcgaa
    49621 tcaccatttt acgggatgga cagacgattg agacactaga aaatgaaatg atcagcgaag
    49681 agcatattat tcgcgggatg gtgggcagag atttagttga tcgttatcct gaaagaaaac
    49741 cggatattgg agaagtttat tttaaagtag aagactggac ggtctatcac cctcttgatc
    49801 acgagcgaat cataaacgac cacatcaact tgcatttgaa aaaaggagag attgtaggaa
    49861 tagctggact gatgggagcg ggcagaactg aattcgctat gagtctattt ggacattctt
    49921 atggaagcaa gatatccgga cgtatattaa aggaaggggc agagatatca gttaagaccg
    49981 ttccttcagc cattgcaaac ggactcgctt atgtctcgga ggaccgaaaa tctttaggac
    50041 tgaatctgct gatggatatt cgggaaaacg tgacgctgag cagtttggat aaaataagtc
    50101 aaaaaggagt attagataaa gaaaaagaag taactgaggc agaaaatttc cggaaaaaga
    50161 tgcgtataaa aacaaactcc atccatcaaa ttgtcagtag tcttagtggt gggaatcaac
    50221 aaaaagtcgt tttatccaag tggctgatga cacaacccga tattcttttt ttggatgagc
    50281 caacccgtgg gatagatgtt ggggcaaaat atgagatcta cacgattatc gaagaaatgg
    50341 cagcaaatgg caaagctgtc tgtcttatct cttctgaact tcctgaaata ttagggatgt
    50401 gtgatcgaat ctatacgatg aacgaaggac gtattacagg agaagtctcg cgagaagatg
    50461 cagatcagga agtcttgatg aaattaatga cgaaagaaga ggtgggttaa gttggaacag
    50521 gcaaaaaatc ttacggataa aaaagagaaa aaactaaatt tgaaaagtag agcagctgat
    50581 ctttttggtc gctacagcat ggtgatcatt ttagcgggac tacttattgc gtttcaattg
    50641 atgacggatg gtattttctg gcgtccattg aatataacta accttgttct gcaaaatagc
    50701 catatattag tactagctgc agggatgtta ttagttgtgc tcttaggtca tgtggatttg
    50761 tcggtagggt cagtgatggc gtttgttggt gcaattgcgg gcatgatgat ggtcaatcgc
    50821 caaatgaacc cgtggatgac agttatcgca tgtcttgcag taggcgggct gatcggcgca
    50881 tggcaaggtt tttgggtagc ttatgcaggg ataccggcgt ttatcgtgac tttagcagga
    50941 ttgctgatgt ttcgtggact gacacaagtt gttctaggag gccaatctct agcacctttt
    51001 ccagctgttt tccaaaaaat gtctacgggt tatttaccag acttaagcga aggaagcagc
    51061 cttcacttct tgacattgat tttaggaatg atcatcacga tcgtgttagt tgttggtcaa
    51121 tggcgaacaa gaaatcgtcg gaaaaatcat ttatttgaag tggaatctcc aacagctttc
    51181 ttggtaaagg cggtatttac agcagtcgtt gtactaggaa taacttatat ctttgcctct
    51241 tatcaagggt atccgattat tttgatcatt cttggtttga tcgttgcagc atatgggttt
    51301 ttgacaaata aaacagtagc aggtcgacag atttatgcga cgggcggaaa tcgaaaagcg
    51361 gctgaactat cagggatcaa aacaaagcga atcacgttct gggtattcgt caacatggga
    51421 gtcatggcag ctttagctgg attagtttta gctgctcgat tgaatgcggc aacacctcaa
    51481 gcaggtacat cgatggaact ggatgcaatg gcggcagtat attttggtgg tgcttcgact
    51541 tccggcggca ttggaacaat catgggtgcg attgtcggtg gcttggtcat gggtgtattg
    51601 aacaatggca tgtcgatttt aggcgtcggg gtagactggc agcaggcaat caaaggattg
    51661 atcttgcttt tagccgttgt gctagacttg tataataaga aaaaacgaac aacgtaggag
    51721 aacgccggtt attcagaaaa aagactgtga caagacattt gtcacagtct ttttttcgct
    51781 aaaagcgaac attcattgtt tcgtttgtat agactaaaaa taatatatgt ttttttacca
    51841 ttcgtataaa ttctcttttt gctcttgatt tttttgctta attgagttaa attacatagg
    51901 gcgctaaaaa aagccttgat taaattaata atgttcgtgt tttgctggat gtgaacatca
    51961 ggaggataat gtaatgaaag tatttgatta cgaagatatt caattaattc ctaataaatg
    52021 tatcgtaaat agccgttctg aatgtgatac aacggtcaca ttaggtaaac atacattcaa
    52081 aatgccagta gtgcctgcca acatgcagac gattattgat gaaacaatcg cagaattctt
    52141 agcggaaaat ggttatttct atatcatgca tcggtttgat gaagcagctc gaattccgtt
    52201 tatcaaaaaa atgaaaaagc gtggattgat tacctcgatc agtgttggcg tcaagaaaga
    52261 ggaatattcc tttatagaga agttggcaga agaatcgcta aatcctgatt atatcactat
    52321 cgacatcgcg catggacatg ctaactcagt tattgatatg attcagcata ttaaaaaata
    52381 tttgcctgaa acttttgtga ttgccggtaa tgttggtaca ccggaagccg tccgtgaatt
    52441 ggaaaatgcg ggcgcagatg caacaaaagt cgggattggt ccaggaaaag tatgtattac
    52501 aaaaatcaag acaggctttg ggactggagg ctggcaactg gcagctctta gatggtgtgc
    52561 gaaagcagct cgcaagccga tcatcgctga cggtgggatc agaacacatg gcgatattgc
    52621 aaaatctgtc cgttttggtg cgacaatggt catgattggt tcattgtttg ccggtcacga
    52681 ggaatctcct ggcgaaacaa aagtcgaaaa tggaatcgtg tataaagaat actttggttc
    52741 tgcttcagaa ttccaaaaag gcgaaaaacg aaatgttgaa ggaaagaaaa tctggatcca
    52801 acacaaagga tctttgaaag atacactagt ggaaatgcaa caagaccttc aatcatctat
    52861 ttcttatgct ggaggaagag atctggaagc cattcgaaaa gttgactatg tcatcgtgaa
    52921 aaattcgatt tttaatggag acacaatcta aaaaataaat aaaatattag gatgttgtga
    52981 aaaagttgtc ctgcattaag taataagaac agccaaacaa aaatagttct ttatatgttt
    53041 tgtttggctg gaattattat cgcagataca agcttttgaa catcattatt cagaataagg
    53101 gactgtgata agatttttgt cacagtccct tatgttttgc aaaaaaagtt tttctttttt
    53161 ctcgtaaatt cggtaaaatc aaaagaaaaa atagacaata gaaaagagaa gcttatgtgg
    53221 gaaaaagtag agaaaaaaca aggaccgatt tatcgtcaaa ttatggaaca gattatgaga
    53281 gaaatcgaaa acggaaggat caatcctgga agtcaacttg aatcagaacg aaaactaggg
    53341 caactttttg gtgtgaatcg ttccaccgtt gtccatgcat tggaagagct tagggagctt
    53401 ggggtactaa cgagcaaacg tggcagcgga tggtttgtta atcagactga atgggggaaa
    53461 ttcgctgttc caagaatcga ttggcggcaa atgatctctc ctcgttatga gcagacggac
    53521 tggtatgagc aaaaaataag agaagcaaag cagatccaaa aaacatcgtt tcttgattta
    53581 tacgctagcg agatgccaga tgagcttttg ccgaattttg agtttccttc ttattctctt
    53641 gaagaaatct tagcggaaga aaaagaaatg gatcttttag gctatactcc gttacgtaac
    53701 aaaatcaaac ggtatttgga aagtaaattt gaattggcat tttcccccaa ccagcttctt
    53761 gtgactggtg gcggtcagca agcaatcttt ttgattttac aaacgattct ttcagctgga
    53821 gaggggatag ctgtagagtc accttctttt ttttaccgat tacctctttt caaagcttca
    53881 ggaacgcgtt tatttgggat accgatggat gaagaaggga tcgacttaga aaaactgaaa
    53941 gaatcgatcc ataaaaataa attgaaagca gtgcttatca acccgaattt tcaaaaccct
    54001 acaggaaaag tgatgagtca aaaacgacga gaaaagctag tagaattgtg tcgacagtat
    54061 cggcttccaa ttattgaaga cgatgtattt acggatcttt catttctaaa ccaagaagaa
    54121 gtccttccta tacgaacgat tgatccggat aatgtgttat acgtcggttc gttatctagg
    54181 gttttaggaa aaacaacgaa aatcggttgg gtgataggac ctgagacttt tatctcgcaa
    54241 ctagctcaag cgcaagaaat gatggagttt tcgatgaata tccttacaca aatcacagtg
    54301 gctaatgtat ttaatgaagc aattgatttg aaaatgcaag atgtaagaaa aaagttgaaa
    54361 gaaaaaagcc gactgataag cgcttgggga gattcacaat cgtttttcaa gcttcattca
    54421 attttaggtg gttactatgc ctggattact tgggatggga aaaaactaga ccccgaacta
    54481 gcagaaaaag tagtagatat gggtcttgga attgcaccaa gtctactttt tgggcaagat
    54541 ataaatggaa tcagaatcaa ttacagtagg ctggatgaaa accaaatacc gttgttttat
    54601 tcaaagatga aaattctaga aaagtggtta acagaataat ttcacaaagt aaatagatta
    54661 aagaatataa cagcttggat ttaaatttca agctgtttct tttttttcgg aaaaagaata
    54721 cgatataaag aaactaagaa aaaaccatta tttgtacgtc cgttcacgat gtgtaaataa
    54781 aataaatgtt tgcaacaaaa ctgtataaca ccaacgtttt taaatagaaa cagatggttg
    54841 taagtttgat tagaaaatag aaaaaatgca gatgaaaaat aaaacgcttt ccttattgtg
    54901 attttattca ctaatgctgt taatatgcgg attggcataa tttcctttca tcgtgaaaat
    54961 ttgtgctttt agcttgtatt tctcataaat ccatgataat ttattaatga agacggttaa
    55021 atagtgatac agttttattc actgtttcaa tcgcagaatg aagagatttt cttatagatg
    55081 agagaggaat gtgtgacaaa tggcaaacaa aaaagcaccg atcgacttcc aagcactgct
    55141 agatgaagtc aatgcagatt tcccagtata tcaagcactt gatcaagatg gaaacgtagt
    55201 taacaaagat ttagtacctg atttatccga tgatgaactc gttgaattaa tgactcatat
    55261 ggtatggtct cgtgtattag accaacgttc aacagcgctg aaccgccaag gacgcctagg
    55321 cttttttgca ccaactgctg gacaagaggc aagtcagctg gctagtgctt acgcatttga
    55381 taaagaagat gttcttcttc caggttatcg ggatgtacct caactagtaa aacatggttt
    55441 acctttatct caagcttttt tatggtctcg aggacatgct gcaggtaatt cttatccgga
    55501 agaattgaaa gctctaccac cacaaattat catcggtgca caatatgtac aagcagccgg
    55561 tgttgcatta ggtttgaaga aacgaaacaa gaaaaatgtc gtcttcactt atactggtga
    55621 tggcggttca tcacaaggtg atttttacga agcaatcaat ttcgctggcg cttaccatgc
    55681 aaatgccgta ttttatatcc aaaacaacgg gtatgcgatc tctacacctc gtgaaaaaca
    55741 aactgcagca aaaacacttg cacaaaaagc tgtcgcagct gggattcctg gtattcaagt
    55801 cgatggaatg gatccgcttg cagtatatac tgtatctaaa atggcacggg aatgggcgat
    55861 cagtggaaat ggaccagtcc taatcgaaac actgacttat cgttatggac cacatacact
    55921 atctggtgac gatccaaccc gctaccgtac aaaagacaca gatgacgatt ggcagaaaaa
    55981 agatccattg atccgcttcc gtaaatattt gacggaaaaa ggactttggt ctgaagaaaa
    56041 agaagaacaa gtcatcgaag caactaaaga agaaatcaaa gcagctattg ctgaagccga
    56101 caaagtacca aaacaaaagg tttcagattt cttgaaaaac atgttcgaag tatctccaca
    56161 atcaatcaaa gaacaaattg caatttatga agcgaaggag tcgaaataat catggcacaa
    56221 aaaacaatga tccaagcaat cacagatgcc ttagcacttg aactagaaaa tgacgagaat
    56281 gtcgttgttt ttggtgaaga tgttgggaaa aacggaggag ttttccgggc aactgaagga
    56341 ttgcaggaaa agttcggaga agaccgagta ttcgatactc ctttagctga atctggaatc
    56401 gcaggattga gtttcggtct tgcgttagaa ggatttcgtc cagttcctga aatccaattc
    56461 tttggtttta ttttcgaagc aatggacgaa gtagtagctc aaatggctcg tacaagatat
    56521 cgaatgagcg gcactagaaa tctacccatc acaattcgtt cgccatttgg tggcggtgtt
    56581 catacaccag aattgcattc ggataattta gaaggtttga tcgctcagtc tcctggtatt
    56641 cgagtagtga ttccttccaa cccttatgat gcaaaaggac tattgatttc ttctattaga
    56701 agtaacgatc cggttgtttt cctagaacac atgaaactat atcgctcatt ccgagaagaa
    56761 gtgccggatg aagcttacga agttcctttg gataaagcag cagttactcg tgagggaaca
    56821 gatgtatcta ttatcacata cggagcaatg gtgcgtgaag caatcaaagc agcagacaat
    56881 cttgcaaaag aaaatatatc tgttgaaatc attgatcttc gtacagttgc gccattagat
    56941 gtagaaacga tcattcaatc cgtggaaaaa actggtcgcg tagttgttgt ccaagaagca
    57001 caacgacaag cgggcgttgc cgcgcaagta gtttctgaga tctcagaacg cgctatcctt
    57061 tcattagaag ccccaatcgg acgagtttct gcacctgata cagtcttccc atttggacaa
    57121 gcagaaaatg tctggttgcc aaatgcaaaa gatatcgaag acaaagtaaa agaaatcgcg
    57181 gaattttaag aaaagaaggg acgaaacaaa gtcatggctt atcaatttaa attgccggat
    57241 atcggtgaag gaatcgcaga aggtgaaatc gtcaaatggt tcgtcaaacc aggagatacg
    57301 atcaatgaag atgatacatt actagaagtt caaaatgaca aatcagtaga agaaatcccg
    57361 tcacctgtta caggaacggt taaaaatgtg attgttccag aaggtaccgt agcaaatgtt
    57421 ggggatgtat tagtagaaat cgatgcacca ggacacgaag acaatgaagg ggacagtggt
    57481 gtagccgcag aatcccaaac tccagctaaa ccggctgctg aacccactgt tgatacagaa
    57541 tcagctggat cgtcaagcga aggagtcttc caatttaagt taccggatat cggtgaagga
    57601 atcgcagaag gtgaaatcgt caaatggttc gtcaaaccag gagatacgat caatgaagat
    57661 gatacattac tagaagttca aaatgacaaa tcagtagaag aaattccgtc acctgtcaca
    57721 gggacagtta aaaacgtgat tgttccagaa ggcactgtag caaatgttgg ggatgtatta
    57781 gtagaaatcg atgctcctgg acacaatagt gcaccgtcaa ctagtgcgcc atcagctgaa
    57841 gcaccaaaag aaaaagtgga aacatcaggt tcagcaagtg tcgttgaagc tgccgatcca
    57901 aataaacgtg tattggctat gccatctgtt cgtcaatttg cccgtgaaaa agatgtagat
    57961 atctcacaag taactgcaac tggaaaaggt ggcagagtaa caaaagagga catcgaaaac
    58021 ttcttggcag gtggtccaag cagtgcacca gcgaaatcag aagcgccaga agcggcagca
    58081 ccgaaagaag cagctcctgc agcagaaagc aaaccagctg caccagcaaa accattcaag
    58141 tccaacttag gagacttgga agaacgtgta gcaatgacgc caacacgtaa agctatcgct
    58201 aaagcaatgg tcaacagtaa gcatacagct ccacacgtga cattgcatga tgaagtagaa
    58261 gtgtctaaac tatgggataa tcggaaacga tttaaagaag ttgctgctgc aaatggtacg
    58321 aaacttactt tcttgccata tgtcgtgaaa gctttaacag cgactgttaa gaaataccca
    58381 gtattgaatg catctatcga cgatgcaaac caagaaatcg tttataaaca ttactacaat
    58441 atcggtattg caactgatac ggatcacgga ctatatgtac caaatgtgaa agatgcagac
    58501 cgcaaaggaa tgtttgctat tgcagacgaa atcaatgaga aagcaaaatt agctcatgac
    58561 ggcaaactat ctgcagaaga tatgcgcaat ggaacaatta cgatcagcaa tatcggatct
    58621 gtaggtggcg gttggtttac accagtcatc aactatccag aagtagctat tttaggtgta
    58681 ggaacgattg cacaacaacc aatcgtaaat gctgaaggcg aaatcgttgt cggtcgtgtg
    58741 atgaaactgt cattaagttt cgatcaccgt atcgtagatg gagcaactgc gcaacaagca
    58801 atgaataata tcaaacgtct tttagctgat ccagagctat taatgatgga aggatgataa
    58861 acaaatggta gttggagatt ttgctgtaga attagataca gttgtaattg gtgcgggacc
    58921 tggtggctat gtagctgcca tccgcgctgc tgaaatggga caaaaagtag caatcattga
    58981 aagagagtat attggcggtg tttgtttgaa tgtcggctgt attccttcaa aagcactgat
    59041 agctgcagga caccattatc aagagtcact agattctgaa atgtttggag taactagtga
    59101 aaacgtcaaa ctagattttg caaaaacaca agaatggaaa gaaaacaaag ttgtccatac
    59161 gttgacttct ggtgttggtt tcctattaaa aaaacataag gttgaaacaa tcgaaggaga
    59221 agcgttcttc gtagatgatc acacactacg cgtcattcat ccagattcag ctcaaactta
    59281 ttcgttcaat aacgcaatca tcgcaacagg ttcccgtcca atcgagattc caggatttaa
    59341 attcggagga cgtgtgttag attctactgg cggattagca ttgaaagaag tacctaagaa
    59401 atttgtgatc atcggtggcg gtgtgattgg tgccgaattg ggcggagcgt acgcaaactt
    59461 aggagcagaa gtaacgattc ttgaaggctc accacaaatt ttaccaactt acgaaaaaga
    59521 tctagtaaaa ttagtagaag atgatttcaa gaaaaaaggc gtaacagtcg taacgaatgc
    59581 aatggcgaaa gaagcagtcg ataacggtga cagcgtgaca gtgaaatatg cagtggacgg
    59641 gaaagaagaa tccgttactg ctgattatgt gatggtcact gtaggtcgtc gtccgaatac
    59701 agatgatatg ggcttagaac aagcgggtgt cgaagtagga gaacgaggat tgatcacggt
    59761 ggataaacaa ggacgtacga acgtacctaa catctatgcc atcggtgata tcgttccagg
    59821 ggcggcatta gcacacaaag ccagttatga agcaaaaatc gctgctgaag caatctctgg
    59881 caaaaaagta gctgtagatt ataaagcaat gccggcagta gccttcactg atcctgaatt
    59941 ggcttctgta ggtatgacga tcaaagaagc aaaagatgct ggtatcgaag caaaagcata
    60001 taaattccca ttttcaggga acggtcgggc attatcgctt ggtaaaacag aaggattcat
    60061 ccgtctagtt acgacgatcg aagataacgt gcttattggc gcgcaaatcg gtggagtcgg
    60121 cgcaagtgat atggtctctg aattagcttt agcaatcgaa tcaggaatga atgctgaaga
    60181 tatcgccttg acgattcatc ctcatccatc tcttggcgaa atcgtcatgg atgcttcgga
    60241 attagcatta ggtttaccaa tccatatttg atttgaataa tacaggcgaa acgcaactga
    60301 aaaatcagtt gcgttttgtt ttttagaatt atcaataata ttattatgta aacttaaaaa
    60361 tatgacaact tcttggttta ttaccacttc ctatgctaca ctgaagagga atatgaaata
    60421 ggagagttga agatgcaaga atatcagtat ccacttgatt tagattggac aacagacgaa
    60481 atggtcattg taatgaagat gtgggaagat ctggaacgtg cgaatgaaaa agggataaac
    60541 aatgaagctt ttttgaagac ttatcagcag tttaaaactg ttgtcaaatc cattggtgag
    60601 gaaaaacgat tgggacggga atttgaaaaa gcttccggtt attctttata tcgtacagtg
    60661 cagcaagcaa aaaaaaatcc aaacaaaact ttgagaatga acgggtgaga taaatggaac
    60721 agatgattga tcatatcaag tcctggcttg aagaagctgg tgagaaaatc aaatcttctc
    60781 aacaaacatt aacaatctcg caaaaatcaa atcgaacgga tctagtaaca aatgtcgatc
    60841 gaaacatcca gaattttcta atcgaaaaaa tccattcctt tgatccagag gcgaaaatat
    60901 tggcagaaga agaagggtgt aatcaattat cggatctcga tggaagagtc tttatcatcg
    60961 atcctattga tggcaccttg aattttgtga ttgaagggga aaatttttgt attatgctcg
    61021 ctgtttatga atccggcgtt ggaaagcttg gtttcattta tgacgtgatg agaaatgaac
    61081 ttttttgggg tggaaaagga ataggcgtgt ataaaaatga tgagccattg cctagaccag
    61141 caaataaatc cttatcggaa ggcttacttg gtgtgaatag ttatcttttc ggtcataata
    61201 aatatggaat ccgagaaatt gcggaaagca gcatggggac acgtatgtac ggatgcgctg
    61261 gtcttgaact agtggcaatc ttgaaaggaa accacattgc ttatatttca aaccttagcc
    61321 cttgggatta tgcagcggga aatgtcttat tggaagagtt tggtatggta acaagcggtc
    61381 tttttggtac tcctttgaca tttgaaaaaa gggaatacta tttagcaggg acttcttgtg
    61441 catatgcaga aatcgcagac cttttgaaaa aagaaggctg aaaaaaaggt agaaatcatt
    61501 cacaaaaatg ataaaaacga gcaaaagatt aattttctcg aaaaaatgaa aacattttac
    61561 tatatttcat gaaaaaagtc tgctataatt agcagtcgag taaaatttgc cctgtcataa
    61621 agaaccaata aaaaaggagt tagttacatt gaattataga aacgatatcc gtaacgtcgc
    61681 aattattgcc cacgttgacc acggaaaaac aacattagtt gatgaactat taaaacaatc
    61741 agatacatta gacgcacata cgcaattgca agaacgtgcg atggactcaa atgcgctaga
    61801 aaaagaacgt ggtatcacga ttttagccaa aaacacagct gtagactata aaggtattcg
    61861 cgtaaacatc atggatactc caggacacgc ggacttcggt ggcgaagtag aacggatcat
    61921 gaaaatggtt gacggcgttg tgttggtcgt agatgcgtat gaaggaacaa tgcctcaaac
    61981 acgttttgta ttgaaaaaag cactggaaca acatatcaca ccaatcgttg tcgtaaacaa
    62041 gattgacaaa ccttctgctc gtcctgagca tgtcgtagat gaagttcttg aactattcat
    62101 cgaattagga gcagatgatg atcagttaga ctttcctgtg atttatgcat ctgcactaaa
    62161 tggtacgtca agcttgtcag atgatccagc agatcaagaa ccaaccatgg cacctatttt
    62221 tgatacaatt attgaaaaaa tccctgctcc tgtcgataac agcgacgaac cattacaatt
    62281 ccaagtttca ttacttgact acaacgatta tgtaggtcgt atcggaatcg gacgtgtatt
    62341 tagaggaaca atcaaagtag gagaccaagt agcattaatt aaattagacg gaacagttaa
    62401 gaaattccgt gtaacgaaat tatttggttt ctttggacta aaacgtctag agattcaaga
    62461 agcaaaagct ggagacttga ttgctgtctc aggtatggaa gatatctttg tcggtgaaac
    62521 agttactcct gtggatcatc aagatgcgtt acctatcctt cacattgacg aaccaacatt
    62581 gcaaatgact ttcttagtta ataattcacc atttgccggt cgagaaggta aattcgtaac
    62641 cgctcgtaag attgaagaac gtttgatggc agaacttcaa acagatgtat ccttacgagt
    62701 tgaaccaaca aactctccag atgcatggac agtttctggt cgtggagaat tgcatttgtc
    62761 tatcttgatt gaaaacatgc gccgtgaggg atacgaattg caagtatctc gtccagaagt
    62821 tattgaaaaa gaaatcgacg gtgtaaaatg cgaaccgttt gaacgcgtgc aaatcgatac
    62881 accagaagaa tatatgggta gtgttatcga gtccttgagc ttacgtaaag gtgaaatgca
    62941 agatatggtc catacaggaa acggacaaat ccgtttgaca ttcttaacac ctgcccgcgg
    63001 tttgattggt tattcaactg aattcctttc aatgacacgt ggttacggaa tcatgaacca
    63061 tacttttgac caatatctgc caatgcttcc tggtcaaatt ggcggacgtc accaaggggc
    63121 gcttgtttcg atcgatacag gaaaagcaac gacttattct atcatgagta tcgaagaacg
    63181 cggtacagtc ttcgtagaac caggtacaga agtatatgaa ggaatgatta tcggggaaaa
    63241 cagccgtgat aatgatttga ctgtcaacat cacgaaagca aaacaaatga caaacgtacg
    63301 gtctgctact aaagaccaaa catctgtcat caaaaaacca aaacaattga cgttggaaga
    63361 atctttggaa ttcttgaatg atgatgaata ttgcgaagta acaccagaaa gcattcgctt
    63421 gagaaaacaa attttagaga aaaatgctcg tgaaaaagca tctaaaaaga aaaaataatc
    63481 tgtaaaagga tgaagagaga aaccagcttg ttataaaaaa tagctggttt ctttttttgt
    63541 ccaactcttt tctgtggaga agtcatcttt gtcaatggac ataaattcag aaaatacgcc
    63601 gaaagacgtt taaaagactt ttaaaaaact tatttctaga gagtttttgt ttcataataa
    63661 gataatgttt cacgaaagga gatcaacttg tccttcgaag aaaatcgaag taaaatggca
    63721 agagaaagga gcgggaagat gaacgactta ttaaaacaat actttggtta tgatgagttt
    63781 agacctggtc aaaaagaaat tatccagaaa gtcattgatc aggaaaatgt attaggaatc
    63841 atgcctaccg gaagtgggaa atcaatctgt taccagcttc cagctttact attagatggg
    63901 cttactgtgg ttgtgtcccc tttaatctct ttgatgaagg atcaggtgga cgcggcgaat
    63961 caattaggta tacccgccac ttttatcaat agctcgttag atggttacga aacagctaga
    64021 cgcttccaag aaatcgatag acagcagtac cgtttacttt atatcgctcc tgaacgtttt
    64081 atcatgccgg attttatcca agcaatgaag cgatggaacg tccgtatgat cgctattgac
    64141 gaggcacatt gtatctctca gtgggggcat gatttccgcc caagttattt acagatggcg
    64201 aaccagctag atcaactgcc aaatcgtcct gtaattgttg ctttgacagc tacagcgact
    64261 gtgcaagtag ctgctgacat aaaaagactt ttaaaaattc cagaaaacaa ccatattcag
    64321 actggttttg aacgagaaaa tctacgattt caagtaatta aagatcaaaa aaaagaacaa
    64381 tatctaatcg aatatttaaa gatcaacaaa aatcaatctg gtatcattta tgcagcaacg
    64441 agaaaagaag tagaccgtct ctatcatttg cttaaaaaat tcgatttttc cgtgggtaga
    64501 tatcatggtg gtctaaatga gaatgagcga acagaaatgc aagaagcctt tttatacgat
    64561 cgcttgcagc tgattgtagc gacaaatgca tttggtatgg gtataaataa aagcaatgtc
    64621 cgttttgtta tccattatca aattcctggc tctctagaag catactatca ggaagctggt
    64681 cgggcgggaa gagatggtct ttccagtgaa gccatcctgc ttttctctcc tcaagatatc
    64741 caagtccaaa agttttttat ccagcagtcc caaagagaag aagggcaaaa gcaaaaagaa
    64801 tatgaaaaat taaaagcgat gacagaatac gtgtatattg agtcctgtct tcagcaatac
    64861 atcctaaact attttggcga gacaagcagt ccgtgcaacc gttgtggtaa ctgtttagat
    64921 gacagagaaa tagtagaagt aacgacagaa gcacagatgg ttctttcttg cttaaaacgt
    64981 atgggtgaga actatggtaa acaaatgctg atgaaagtct tagcaggatc gaaagaacaa
    65041 aagctgcaag ctttaggttt tggacattta tctacctatg gactgatgaa aaaccaatcg
    65101 caaaaagaaa cgatgcagtt aattgaatat ttgatatcga atggctatct tctgaccatc
    65161 aatggtgagt atcctgtttt gaaagtaaca gaaagaggga tacaagtatt gaaaggacaa
    65221 gaatctgttt atcgaaagga acccaagaaa gtccagcagc tatcggatga agaaaccgat
    65281 acactatttg aagtgctgcg ggaactgcga acggatctag catcagaagc aggcgttccc
    65341 ccgtatgtcg tattttcaga ttccacattg aaagaaatga gccgaatacg tccatcaagc
    65401 agattggaga tgcttcagat aaaaggagtt ggccaaagca agcttgataa atatggcgaa
    65461 gcctttttat ctcggataaa aaatgcggac tctaacgttc tggataagta gaaagaacga
    65521 atacgtaaaa atgaataaaa acaactggtt aaatctgttt tttttgctcc ttatgaaaat
    65581 caaatatgtt ataattaagg aattagaatg ggaggtaagc acagatggat tctttgtcga
    65641 aaaaacttgc gatccaagta ttaaatgatg aagcagaacg gatcaaacga ttaatccgaa
    65701 atcaaaacaa cagtctctgt atctcacaat gcaaagcctt cgaagaagtg gtagatacac
    65761 agatgtacgg cttttcccgg caagtcggtt ttgctgtccg tataggagta atcggaaaga
    65821 acgagggcca gctgatgtta agtgagctag aacgagaatt gaatcagtta tatagtgagg
    65881 tttaccaaga aatttatgat aaaaaagaag ttggcaagga ggaatagact tgcctaagaa
    65941 agtgaaaaaa aggcatctat tagactatag catcttgatt ccctatttaa ttttatgtgt
    66001 attgggattg atcatggtat atagttctac gtcgtatcta ttattagaaa acggacaaaa
    66061 tccgtccgca tcagtcatta accagagtat tttttgggtg cttagcttga ttgctatcgc
    66121 actactttat aaaatgaaaa cagatgtttt gaaaaaccag cgtttgatca tggcagccat
    66181 cgcagttttg acgattttgc tgctgattgt tgtgtttttt ggtaaggaga tcaatggggc
    66241 gaaaggttgg ctgcaaattg caggattctc gatccaacct gccgaatacc taaaaatcat
    66301 ttctatctgg tacctgtctc tgactttatc taaaagacaa aacagtgtac aaaaagattt
    66361 tcttgggact gtgaaacgtc cacttgcaat ggttatcgga cttactgcat tagtcgctat
    66421 tcttcccgat tttggtaatg cggcagttat ctttttgatt attttggttc tgcttttagc
    66481 cagcggagtc aattatgtgt atactttgat tgtaggagta ggaggctttt gtctaagtac
    66541 ttttacgatc tggctgatca atatcacaaa cggaaagatc ttacccggac gtttacagta
    66601 tatctataat cgatttgcta tttaccaaaa tcctttctca gacgaattaa ataaagggca
    66661 tcaactagtc aacggctact atgccatgtt caacggagga ctatttggca ggggattagg
    66721 taacagtatc cagaaaaaag gatttttaca agaagcacaa accgatttta tttatgcaat
    66781 cgtagtggaa gaactgggag tgattatggg gattttgata ttagcgttgc tattctttat
    66841 gattgtgcgc attgtccttg taggtatccg ttcaaaagat ccgttcaatt cgcttttgtg
    66901 cattgggatc ggcgcaatgt ttttgatcca agtatttgtc aatcttggag gaattactgg
    66961 cgttattcct ttgacaggga tcacgttccc atttttaagt caaggaggct ccagtttgct
    67021 gatgctttcg atttgtgtcg gctttgtcct caacatcagt gcggatgaaa aacgcaagag
    67081 cttaggttta taataaaaaa agatgtagga gtggttcgat gaaaaaagtt ttagttgcta
    67141 acaggggtga aatcgctgta cgtgtatttc gtgcttgtac agaattagga attaaaactg
    67201 ttggaatcta tgcagaagaa gatgaatatt ccgtacaccg tttcaaagcg gatgaagcat
    67261 atttagtagg acaaggaaaa aaaccgattg atgcgtatct agatattgaa gggatcattt
    67321 ctatcgcgaa agaatgcgga gctgacgcta ttcatccggg ttacggtctt ttgtcggaaa
    67381 acttgaattt tgcgcaacgg tgcgaagaag aaggcatcat ttttgttgga cccaaactgc
    67441 atcatttaga tatttttgga gataaaatca aagcaaaagc tgcagctatc gaagcgggga
    67501 ttgcttcgat tccaggcaca gatggaccaa tcgcttctat agacgatgcc ttggagtttg
    67561 cgaaacaata cggttatccg attatgatca aggcagctct aggcggcggc ggacgcggca
    67621 tgcgtgtagc tcacgatgaa aagagtgcaa gagagggcta cgaacgtgcg aaaagcgaag
    67681 caaaagcagc ctttggtagt gatgaagtgt atgttgaaaa atacatcgct aatcctaagc
    67741 atatcgaagt acagattctc ggggatacgc atggaaacgt catccatctt tttgaaagag
    67801 attgttctgt tcagcgccgt caccaaaaag tagtagaagt agcgccttgt gtctctatga
    67861 atgaacagca acgtcaaaaa atctgccagg cagctgtcca attgatgaaa tatgtcgggt
    67921 atgtcaacgc aggtacagta gaatttcttg ttgaaggaga tgatttttac tttatcgaag
    67981 tcaaccctcg cgtccaagtg gaacatacga tcacagaaat gattacagat attgatatcg
    68041 taacaacgca actgttgatt gctcaaggtt tggatctgca taaagaaatc ggattgcctc
    68101 agcaagaagg aattaaatta aatggttcag ccattcaatg ccgaatcact acagaagacc
    68161 cgttgaataa ctttttacca gacacaggga aaatcgatac gtatcgttca cctggtggtt
    68221 ttggcgtacg tctggatgtc ggaaatgctt acgcgggcta tgtagtgaca ccgtattttg
    68281 attcattgct agtaaaagta tgtacgcatg gtgctacctt tgaaacagcc attcagaaaa
    68341 tggaacgttg cttgaaagaa tttagaatcc gtggagtgaa aacaaacatt ccatttatgc
    68401 tcaatgtcat cacacatcct gaattccaat caggaaatgc aaaaacaacc ttcattgata
    68461 gtacagcaac attatttgaa tttcctcgac tacgagaccg cggaaacaaa acgatgaaat
    68521 atatcggtga aattactgtg aatggttttc caggtatcga aagtggcgaa aaaccatttt
    68581 atgaagaacc acgtatgcct aaagatctaa tcacaagaac tgattacgta acggctaaga
    68641 atgtattaga tgcgaagggt gccgatgctt tggtcgaatg gatcaaagga caggaaaatt
    68701 tgttgttgac ggatacgaca tttagagacg cccatcaaag tctgctagca actcgtgttc
    68761 ggacaaagga tttcaagcag attgcacgtt tgactggaga aggtcttcct gaactctttt
    68821 caagtgagat gtggggggga gcaacatttg atgtggctta ccgctttttg aatgaagatc
    68881 cttggcaacg gttgagaaaa attcgttctt taatgcctaa tacgttgctg caaatgcttt
    68941 tcagaggttc aaatgccgtt gggtattcta attatcctga caatgtattg gtggaatttg
    69001 tcaaagaagc agcagcgcaa ggaatcgatg tatttcgaat ctttgatagt ttgaactgga
    69061 ctccacaaat ggaaaaaagt atccaagctg tacgggatac tggtaaaatc gcagaggcag
    69121 ctatttgcta tactggcgat atcaacgatc caagtcgggc aaaatacaat gttcagtatt
    69181 ataaagacat ggcaaaggaa ctagaacaac tgggtgcgca tatcattgca atcaaagata
    69241 tggctgggct gcttaaaccg caggcagctt atcgtttgat tagtgaattg aaagaaacga
    69301 cagatctgcc aatccatctg catactcatg ataccagcgg gaatggaatc atcacttatt
    69361 ctgctgcttc aaaagcagga gtagacatcg ttgacgtggc aatgagtgca atgagtggga
    69421 atactagtca gcctagcatg agcagcttgt attatgcact ggtcaatggt ccacggctgc
    69481 cagaaatcac gattgaaaat gcacagaaat taaaccatta ttgggaagat gtgcgcatgt
    69541 attacaaacc gtttgaaaac ggcttgaatg ctcctgaaac agaagtatat atgcatgaaa
    69601 tgccaggagg acagtattcg aatctacagc aacaggcaaa agctgtcgga ttaggtcatc
    69661 gctgggatga aatcaaacaa atgtatcata ctgtcaactt gatgtttggc gatatcgtta
    69721 aagtaactcc atcatctaaa gtagttggag atatggcact atttatggtc caaaatgatc
    69781 ttacagaaga agatatttat gaaaaaggcg aaacactaag tttccctgaa tctgttgtga
    69841 ctttcttcca aggagaacta gggcaacccg ttggcggatt tcctaaaaaa ttgcaaaaga
    69901 ttattcttaa aggcagacct gcattgaatg aacgtccagg tcttttggct gaatcagtcg
    69961 acttcaacga agtaaaaaaa gaattggcag aaaaaattgg ctatgaacca aaacaagacg
    70021 aagtattaag ctatttgatg tatccgcaag tctttttgga ctatcaaaaa gcatataatc
    70081 aatttggcga tgttacatta ctggacacac caacattttt ccaaggtata cgtttgggtg
    70141 aaacaatcaa tgtacaaatc gaacgcggaa aaacgcttat cattcgttta gatgaaatcg
    70201 gagaacccga tatcgaaggg aatcgcgtac tgttcttcaa tttgaatgga caaagacgtg
    70261 aaatcactat caaagataat tcgatcatca gtacggtcca aacccgattg aaagctgaac
    70321 cgacaaaccg agaacaaatt ggagcaacga tgtctggttc tgttttggat gtattagtga
    70381 aaaaaggcga taacgttaaa aaaggtgata cgttgatgat tactgaagcg atgaaaatgg
    70441 aaacggccat cgaagcacgt tttgacggag aagtcgcaca tgtatatgtg tcttcaggag
    70501 acactatttc gtctggtgac cttttgattg aagtgactga gaaataataa gcgaaaggtg
    70561 gggaaacatg aaaagactgg gtttgttttg gctgattctg ctattagtta ttgtggcatt
    70621 ctacatggaa ccagtcttgt ttccgccaaa acttgaagtg aaggaaaact ctgatcaaca
    70681 aatatcaaac caaatgacaa cgtggaaata ccaggagctt aaagcaactg gtttttctac
    70741 ttatatcgga aaaccgatcg atcagctgga aaaggatttt ggccaagcct atgatcgatt
    70801 aactagcgga tttggttttg aaacaagata ttatgaaaat aaagctagtc atctcacatt
    70861 tgaagcgaac atccaagaca gcagagtcac aactgttaaa gtactgcaaa cagggcctgc
    70921 tgatatcgcg ccgttttatt tagggatgac gatgcaagat cttacgaaaa tcactactat
    70981 ctatactaat ttcacttttg agtaccatga tacagaagtt ggaatcgaac taatggaaga
    71041 ggatatgaat tatcgtccgc taattgcttt tgataacgac acttttgcta ttttattttt
    71101 tgatcaaaca acaaacggcc tattttcagt tgtgtattta gataaagata gtcttttgaa
    71161 actattgcca tatcaagtgt ttggtgaagg ccttccacat tatcaagcag ataaatcggc
    71221 tgattgggaa tcaatcaatc gcgttaaaga acgaaaaagt acgactttat taaatatgct
    71281 tagaagagaa gacgaacttc ccatatacaa tcaaacactt gcctttcaaa accacagtca
    71341 gctgctacta catgattttt tgacaaggcc tgaggaaata ttgtcagagg aacgtatggc
    71401 tgattggcaa aaatcgctag gcagtgctca aacgacagtg aaattctcac tgacgaatga
    71461 tgaattggag aagttaatca gtaaacaaaa attagaaaat actagtgggc tatttattca
    71521 tccggcgatt gacgctactt tttcgttttt atattggtac agcgatcctt acaaccatga
    71581 ccgtttcatg gacccgggaa cggattctgt aggcattgct ttttctaaag aaaatatgtt
    71641 agtcttattg caagaagaaa acggagaaag tagtgatagc agtgatcgtt aacgaagagc
    71701 tgtttcaact ggaggatcaa tgcctccaag tcgctgaaat gataaaaaag agtaaaacga
    71761 tggcttctta tcttgaacat aaacagtcaa tggaagaatc tgcaaaagtt tgccagttga
    71821 aagaagcgtt cttgtctaag aaagaagcat ttgaaaatgt agcggcatat ggtgagtatg
    71881 cacctgatta tcgtgaaaaa aaacgagctg ttcgaaaagc aaaaagacag cttgatatgg
    71941 atgagcatgt tgcagctttc cgatatagtg aaacacagct gcaaagtctg ttggatcaaa
    72001 ttgtccaagt acttgccgaa gaagtttcta atgaaatcaa agtagatgca ggtagcccgt
    72061 tttttgaaac gaaaaaacac tttggatgcg gaggtaattg tcatgcaatt agacaatagt
    72121 gaatttgaaa taacaaaacg acgcgggctg atcgtatggg tctacagctt aaaacaatta
    72181 aagaatctga aaaagttcgg cttgttgcat tatgtctcaa gaagaatgaa atatgtcgtg
    72241 ctttacttaa atgaagagac attggaagaa acagaagaac gaatccgtaa attacatttt
    72301 gttcgaaacg tagaacgttc ttaccgaccg gatatcgaaa tgaattttgc cgaaaaaata
    72361 ggaaacaaaa cagtattaaa agacgaaggt ttcgaaattg aagagctaaa tacagaaatc
    72421 cgattagctg aacatcttta aaaatctctt atgagcagtt tttaaataaa aaataacttg
    72481 gataacgatc gatttttgat cattgtccaa gtattttttt ggtatactag taccagtacc
    72541 atttctaagg agtgagtaga tgcggataat atcaggaaat tatggcgggc gccgtttaaa
    72601 aagtttagca ggggcgaaca ccagaccaac tacagataaa gtaaaagaat ccatattcaa
    72661 tatgattgga ccatattttg atggagagac agtattggat ctgtttgcag gaagcggtgg
    72721 tttagctatc gaagcagtat ctagaggctg cagtcatgcg gtatgcgtag acaaaaatta
    72781 ccaagcgcta aaaatcatca aagaaaacat cgaaattacg aaagaaccag agaaatttac
    72841 cacaataaaa cttgatgcgg acaaagcaat cgaacggcta gcagcggaga aacaacaatt
    72901 cgactacttg tttcttgatc cgccatatgc aaaacaaaaa ataatcgatc aaatccataa
    72961 aatggaagaa ctgggacttt tttctaaaac cgcagttatc gtatgcgaaa cggataaaac
    73021 agtcgaattg ccagaaactg tggctggatt ccatcaaata agaaaacaga tttatggcat
    73081 aacggccgta actatctata gaaaagaggg aaatcaatga aacgtgcatt attcccagga
    73141 agttttgatc cttttacaaa aggtcactta gatacggtgg aacgtgcggc taaattattc
    73201 gatgaggtag taattggtgt ttttatcaat acttcaaaga aaagtttgtt tcccccagaa
    73261 gaaagaatga cattgatcac aaaagctgtt tctcatcttc ctaatgtaaa agtcatgcat
    73321 caagaaaatc aattgacggt agaaacagca aaagagattg gtgcagatgc gttgatcaga
    73381 ggcatacgaa gtatcaaaga ctttgaatat gaacgagaga ttgctcagat gaatcatcat
    73441 ctatatgatg aattggaaac tgtcttctta ttagcaaaac cagaatatag tcacgtgagt
    73501 tccagtattt taaaagaagt actgcacttt ggcggagatg tgagcagcta cttaccgcca
    73561 gtgatcaacg aagctttagc tggaaagcgt gaatcaaatg aaagctaaac aatggttgaa
    73621 aacagtactg attatcatat ttggaatgtt gatcattgga tttgtagtag ttcccttgcc
    73681 gtattatgta gaatcaccgg gagccacgat cgatctgaaa gaactaatta cggtcaatga
    73741 taaggaagat aaaggttctg ggtctttctc tcttacctct gttggtatca ggcaagctac
    73801 aggtttctta gctttaaaag ctaaaatgac cccttttgaa gaattgatca gtgaagaaga
    73861 attgactggt ggggcgacaa atgaagaata taatcaaatg caacagtatt atatggattc
    73921 ttcccaaaat gcagcaatcg aacaagcgtt aaaacttgca gacaagcctt ataaaatgga
    73981 attcaaaggc gtatatgtca tgagtgtaga gaaaacatcg aatttctttg gcaagctttc
    74041 agttggagat acagtaacaa aagtagacgg gaaaagcttt caaagtacag aagcatttat
    74101 ggattatgta aaaagccaaa aagttggaca aacaatcgaa atcagttatt tgcataatgg
    74161 tgaagaaaaa acagcttctg gtgatctgat cgagttacca acagataaaa aggcaggaat
    74221 aggtatcacg cttactgacc ataccgagat cgaatcagat acagatgttc gtattaattc
    74281 tggctctatc ggtgggccat cagcgggatt gatgttcact ttagaaatct atgaacaagt
    74341 cactggtaaa aacttgcgac atggtaagca aattgcagga actggaacga tcaacagtga
    74401 aggagaagtt ggccgaatcg gaggaatcga taaaaaagtg gcaagtgcgg ataaagctgg
    74461 cgtagagatt ttctttgcac cagatgatac gatttcagac gatgttaaga aagaatatcc
    74521 tgaaataaaa accaactacg aagaagcaaa agctgccgct aaaaaattga attcctcaat
    74581 gaaaatcgta ccagtaaaaa cagtacaaga agcgcttgac tatctagaga aaatgaaatg
    74641 aaactaaata attaaagagg ggttgtgaca cgagttctgt cacaacccct ctttttcgtc
    74701 aaaaatcttc acgataaaaa taaaaacaaa agaaaaaatt gacgtaatta gatgttgaag
    74761 ggacgatttt gccagagctc actgtttctt caattttaaa tgaaaggagt aatgaatgat
    74821 ttttactggt cattctttga aaaaagaaca atgatagaaa aaatcagcaa gaaaatacta
    74881 gcttcaccca aattgatcat ttggatgtct atgatagttc ttatcttttg tatcgcaagt
    74941 ggttttttat tatcaaattt gctattttat agagaaacag aagagactgc tgcaaaagaa
    75001 atactttctt cgtttagttc cagtggagaa gatccatcag ctgaaggaaa cgatgtagaa
    75061 gaggatgctc gtccaaaggt catgtacacc gatatcaaag gaagtgtgaa ggagcctggt
    75121 atctattcct tttcttcaga agaacgggtc tatgatgtac taaagcgtgc aggtggatta
    75181 ttagaagaag cggatagcga tcgaatcaac ttttctgcga agatagaaga tcagcaagtc
    75241 ctatatatcc ctgctgtagg tgaagagctg ccagaacatc ttgatcagtt ggcatcctcg
    75301 gaaggaaaac agcctacagc agattccgag cctcccaaaa taaatatcaa tacagccact
    75361 ccatcagaac ttcagcagat tcctgggatc ggatcagtca aagcgcaaga aatcattcga
    75421 tttagagaag aaaacggatc cttccagaaa gtggaagacc tgcaggagat ttctggtatc
    75481 ggtgaaaaga cagttgaaaa actaaaaaac ttcgttacaa ttaaatagaa tgattcaaaa
    75541 tcgactggca cgagcaataa atggacgcta atggatacta agaataatcc aattctaaga
    75601 gcttggcagg acgaggaggt agtatttttg gataggaaat agcttgattg ttcgcaaaga
    75661 aacattaaaa aaaccaatgc tgaatgtcta tctccaaaat aaaatatctg ggattcatat
    75721 tatgaatacg gctgtcagcg gcaataacag tcaagctcta cgagaacgct ttgccaaaga
    75781 tgtacttagc tatacagcag ataaagtatt tattctcatt gggacgaatg atctagcaga
    75841 gcataaacaa ctttctaaag aaacgtacca aaaaatctgc agtggatgat agttcagctg
    75901 aaaaaagact atagttctga tcatgctttc tttatcacgc caccagcagt cgatgagaca
    75961 aaacaaaaat atcggaataa caagttgatc aaagaatata tctctgttct cacagaagta
    76021 gcaaaacgcc atgattgtga agtatcgatt tttatacgga atttcttgaa caaatggatt
    76081 cgaagaattt ttataatggg ctaatggatg acggacttca tttcggcagt gaagggtagg
    76141 atcttttgtc agacaggatg attcgttata tttaaagaat attttgttta agcaatgagt
    76201 actttttaat caacttgaaa aaacggagga atataaaaca tgttagaaaa atcttcaaaa
    76261 gcatgcgata cgaataaaga aaaagttact gataagtcac ataatgaacg cattccttgg
    76321 gaccagtact ttatggctca ggcagtactg ttatctttac gtagtacgtg tacacgttta
    76381 gaagtaggag cgacactcgt tcgagaaaaa aggatcattg ctggcggcta taatggagca
    76441 gtcagtggag atgtccactg catagatgaa gggtgttacg tcgtggatgg acactgcttg
    76501 cgaacgatcc atgcagagat gaatgcactc ctacagtgtg caaaacttgg tatcccgacg
    76561 gaaggttcag aaatctatgt cacacatttt ccatgtttgg cctgtacaaa ggctttgttg
    76621 caagctggtg tgaaagggat caattatctc catgactatc gaaatgatcc ttatgcccaa
    76681 gcgttgatcg aacaattagg tgtatctgta caccaagttg ctttagactc acagcatttt
    76741 tcaaaacttc aaaatgaatt aacaggaaaa catgtctagc gttttagaaa ggcatgttcg
    76801 aaatcggttg atttttccgg caattacgtc gatgattatc tgtttgttgt attacactga
    76861 caattggggt tatcgtttgc ttcttgccgg aattttttta tggttttcgc taaaagaagg
    76921 acgttttatc gcgtacttgt gtattttgtg tagcataacg gtatccattt cttataggct
    76981 acaagagtat tcatttttag aggaaaaaac aacatctgcg caaattactg tatttacaga
    77041 tacgattcgt gcaaatggta attttttgac aatggaagga aggttatcag agaataaaca
    77101 aaagctccag ctttcttatc aagtaaacac agaagcagaa ttgaaaacat ggcttaactt
    77161 ccgaggaaag ggaatagaat tgactgctga aggcgagttc atctctccag caaaaaagcg
    77221 gaatctttat aattttgacc aagaagctta cttcaggaac catagaattt ctggacggtt
    77281 ccaaattcaa aagattgaac agatcaccat gcaaaatcac tggcgaaact ggcttcagag
    77341 aaaaagagga tcttttatct cctttgtcca atctgaattt ccgcaaaaaa caagtgtata
    77401 tattaattct ttattatggg gctataaaga tgcaggattc agagaaccag aagaaatttt
    77461 tagggagagt ggtttgctgc atctgttcag tctatcgggt ctacacatcc aattttattt
    77521 agggtggctg tattatttat ttcgacgtat cggatggcca ctacacgtca gtattattcc
    77581 gttgagtcta ctaactatct gttatgtttc tattgcagga agttctattt ctgttttgcg
    77641 tgctagtttg ctgtttttat tgcgattgct agtcaaattg ctgaataagc attattccac
    77701 catggactta tttgcagttg tcttttggct tcttttacta tttgatcctc gatttctttt
    77761 tcatgcgggt ggacagctta gttttttcat gagttttttg tttatcctga tttcttttga
    77821 tggagattgg ttgaaaaaaa cggcctctca cgtcctgttt actatgatca cgatgccgtt
    77881 gattgtatgg catttttatg agtggcctat tttaggtagt ctttttacat tactgattgc
    77941 accggtattc aagtttcttt ttcttcctgg tgtgttattt ctcacactct tcaatcgctg
    78001 gctgccggaa actttacttt tgctgataga agagggatta atcttatttg agcgaataat
    78061 cgatttttct tcagggtcgt ctctcattat aggacgtttg ccccttgtgc tatctgggat
    78121 gtatattttc ggattactga tttgtatgga taggttgaaa gaacaacctg tgaaacactt
    78181 gttttatgtt ggtctttttt cttttatttt tttgactttg ccatttttac gcttttcttc
    78241 aacgatcgct tttatcgatg tcggtcaagg agacagtatc tttttgcaaa gtccttttcg
    78301 aaaagaaacg attttgatcg atactggagg tcagttacac tttgaaagag aaaagtgggc
    78361 gcaaggtatg atacgtcctc ctgctgaaag aaatatcgtt ccatatctaa aaggtcaggg
    78421 gataagtgta atcgataaac tcatcttgac acacgacgat acggatcatg tgggagaatt
    78481 acccacgtta tctcagcatt ttactatcaa aaaaatttat atcggttggg gcgctggacg
    78541 aaatgagcca ttacaaaggc atttatctga agctcaaaaa aatggtactg agatcattga
    78601 aatcaaacat ggggacagga tcagcggtta ttatgatctt tatgtattga ctccttttga
    78661 aaagggagag ggaagaaatg aagattcgat tggtctatgg atggaatata acaataggcg
    78721 ctttttattt ttaggagatt tgaatcaagc aatggaaaaa caattgcttt tgatttatcc
    78781 taatctgaaa gccgatgtag tcaaattagg tcatcacggt agccgtacgt caagtaacac
    78841 agattttctt tcccatattg aagcaaaaca tgggattata tcctgcggcg taaataatcg
    78901 ttatggtcac ccgcacagcg aagtattgga gacactagaa gaaaatcaga taaaaacatg
    78961 gcgaacggat caacaaggaa tgatcaccta tcgctggcat ccagtttttc atcctcatgg
    79021 gctagtggaa acaatgatag actagtaatt ctcatctgct catggtagga taatttttga
    79081 aagtggtgag taatttgagt atacaagcag agttacaaaa aatacgtaaa gaaaaacttg
    79141 catcttgtta cttgatttta ggaacagaaa agtttctcca agatcaagtt cggacagaaa
    79201 ttgtcaaaaa aatccaatta gagggcgaag atgacttaaa ttttttaagt tttgacatgg
    79261 aaaatgcaag tttagatgag gtagtagctg aagcagagac gttgccgttt ttcggagatc
    79321 aacggttagt attcgtagag aatccttatt ttttaacagg tgaaaaagta aacaatggaa
    79381 tcgaacaaaa tactgattta ttgacggctt atttgaaaga accattagaa tcgactgtat
    79441 tagtattctt tgcgccatat gaaaagttgg atgaacggaa aaaagtaacg aaacaattga
    79501 aaaaaacagc aattatcatc aatgtccaac agttgaatga aaaagaagta cgtcaatatc
    79561 tgatgaatac cttagagaat tcctcaatca aaatggaacg aagtgcaatt gatttattct
    79621 tgaggttgac cgacttagat ttatccaaaa tgatgagaga gttacagaaa ttggtccttt
    79681 atggacaaaa tcaacagcaa atcacggtaa gagaagtaga agaattagtt cctaaaacgt
    79741 tagaacataa tatttttgac atgacccaat atattttaaa agggaagacg gaacaagctt
    79801 tgcgtttata cgaagattta gttatgcaag gggaagaaac aatcaaaatt aatgcgattt
    79861 tgctttctca attacgttta tttttacaga caaaatttct tgtaaagatt ggttatcagc
    79921 aggcaaatat tgctgaaaca ttgaaaatcc atccatatcg tgtaaaactt gccatgcagg
    79981 aagtaaaaaa attcgatgaa catttgctgg tacagttgtt tgatcaatta gttgagatgg
    80041 attatcaaat caaaacggga caaatcgaaa aagaattaag ttttcaacta ttcgttttgc
    80101 gcacagggca gttgttaaga aatcgataag aagaaagacg agggcaaagc atgcgggaag
    80161 gtcgtttgtt tttgtccttt ttttgacaaa taaaaaaatc ggccgaagcc gattctttta
    80221 tttagcgatt tttttgctta aacgagattt gtcgcggtta gctttgtttt tgtggattag
    80281 accttttgat tcagccatat caatagcttt aacagcttct ttatataacg catctacgtt
    80341 gtcagcacct gaagctacag catcttcaaa ttttttgatt gcagtacgca tagcgcttgt
    80401 ttgagatgaa tttttcacat tagcgttttc gcttgtacga acacgtttga ttgcagattc
    80461 aatgtttggc attagtttca cctccgataa cttaagttct tacgcgtgtt accgcataat
    80521 tcaacattca ttattatacc taaaggcagc gtgctttgca atatgaacat gacaagtttt
    80581 tttccttaac agtcttttat taggattttt tttcagtaaa aacggatagt acaagctgaa
    80641 aaaaatcaaa aagacgctat tgtgggaggc aatagcgtca aaccaaacta gatttttatt
    80701 tatctattta aacaaagttt gaatgggaat gtttggtact aagttattat ataacaaaga
    80761 gaaccgtttg caaagttttt tttgaaaagg gatacgattc cctgttgttc tttgtttttc
    80821 tattaagaaa ctgcagttat tttataacca gtatcctttg atcagtaaag gctattctgg
    80881 tgtaccgatt attttaatgt attcatcgat attttttatc aacatttttt ttatgaccaa
    80941 ctggttccga cggttccatt tgccacttac ttccagcaca tatttattta ggggcaaacg
    81001 taagatatat tttgccaaat agcgatcttt aagcaggcca ttgatcacac ggtcctcaag
    81061 ctgcaagttg atcccagtca gcatcattgg ctgttcttgt aaaacgtacg tatcaattaa
    81121 aagaccttca aaagtttcca caagcgtctt cctttctttt caaagtaacc tgaaaacagg
    81181 cactaattct tccattttga tattcgttga agtggatgcg atcattcttt cttttgtcca
    81241 ctgtaatcta gtataacaaa aaagagcaga agagccaaaa agaaaaaaga gcatcgaatt
    81301 ctataggaag tctcattggc acggcctaaa ttattacaaa acattgatta aaaaaagccc
    81361 ctgcatcgca ggagcatccc ctatatcgtt ttaataggtc aatcaaaaag cagtatcaaa
    81421 cacattttgt atcaaatcag gaaataatag ataagctaag cccagaatca aaagtaccgc
    81481 tagaagaaac cagccgattg ttcctatgaa atactttaat ttttcgaaca ttgtatcacc
    81541 tcgttgtcag tatatcacgg atcagtaaat tgggggtgtt aagaatctcc taaaatagac
    81601 aaaatttgtt ctattttcct aaagcaaata cttgtaagat acagtaagtg ataccagaag
    81661 caatgaccgg tccggcggcg attcctctta agaagacaac acctaaaatc gtgccgaaaa
    81721 caagtgcaac agtgatttgt ggatcagaag caatcaatcc tacacccttg gaagataaga
    81781 cagccactag cccaccgcaa aaaacagcta cccagccaat aggggattta aaggcttcta
    81841 atagatcacg tagaccgatt tctcctgttg cgatagggat tagaatagcc gctgtgatga
    81901 tcgtcacccc aatattgatt cctttgcctt gaagccaagg gaaaaaggaa cgggaggctg
    81961 gtatgagctt cataatcaaa acaaaagctg ccgctatcat cagactttga tttttagcta
    82021 aaattgctac aagtaaaata attcctaaaa aaagccaact ttccatgaaa tatattcctc
    82081 actttcttcg aaacaatcat aacatgtttt tagaaagtta caaaagtagg aattcatcct
    82141 tcaagaccta ttcataagct gtttttttca taaaataaac aaaatcataa aaaaacactt
    82201 tgcttatttg acagaagaca gccgcgcttt gtatagtgga caagacttac atttttttga
    82261 agaatcagaa agatcaggta aaggtcttag taaacgcaag ccattcagaa taaccaatat
    82321 tgtgcttcct tcgtgtccta caactccaag aggtagattt aaaaattgca ggaagttaga
    82381 agcaatcaac aagagaataa caagaatcga aaaaataata ttttgtttga tgatccgccg
    82441 catttttaaa gatagccgat gactgtagac aaatttattt aagtcgcttt tcattaagac
    82501 gatgtctgcg atatccatag cggcatctgt cccttgcccc atagcgacac ctaaagaagc
    82561 ggctgctaaa gcaggcgcat cattgactcc atcaccaacc atcgcattta cttggtaaag
    82621 ttcattctct tttttgacta gttgtgtttt ttcttccgga gtacagcttg catagtaatg
    82681 ttcaatccct acttgttcag caatggcctt tgctgtgcca gaatggtcgc cggttatcat
    82741 acttgtatgg atgttttggg atttaaagta ggaaattact tgttgggtat ttgcttttgg
    82801 aatatctagc agacctaaaa cagcaattaa ctgctgatct ttagaaagat aaatcacggt
    82861 tttgccttga ttttccaaac gttcgatcat ttccgcaatc tctttgctga ttatcatcgt
    82921 ctcaggatca tatgcgtgct tgccaacttt ccattgcgca cctaaatagg tagtttgtaa
    82981 tccaaaacca gtaatttctt ctattggtaa gtattggatt tcttcaggta ttgtgcaatc
    83041 gaaatattgc aaaatcgcat gagcaagtgg atgcgtcgtt ttttgttcca tagcaacaag
    83101 aagttgttgt gccaaatcct tttcatcaaa gaaaaagctg tcagttacaa ctggttttcc
    83161 tttagtgagc gttcctgttt tatcaaaggc aatcgctttt aattcagaaa gcttttctaa
    83221 atggataccg cctttgatta aaatcccatt ttttgcagaa tttgaaagag cagctagaga
    83281 agctggtgta gcagatgcga caagcgcaca aggagaagcc acaactaata ataccattcc
    83341 tcgataaaag ctttcatccc aactccaacc taggaaaaag tgggggagaa ggatcattag
    83401 aggaacagca agcaaaatca attttacata attattttcg attcgttcga taaatgaagc
    83461 tgtttgagtt ggcgtattct gcgcttcttc tactaattgg ataatcttag caaagacagt
    83521 ttcggaactc gtttttgtta ctcgaatcgt gatgggattt ccttgattca gcgttcctgc
    83581 atacatctca tcgcctaaat atttttctac aggcacagat tcaccgctta atgcagactc
    83641 atcgatcgtt gcctgtgtgc tttctaaaaa gccatctatc ggaacagctg ctcctttagc
    83701 gacaaatacc aagttatcca cctttagttg agaaacatct acttcttgca tttgtccatt
    83761 ttcttttaat aattgggctt tttgtggttg aagattcatc aaagcagtaa tttctttttg
    83821 acttttatta gttgtgtatt cctctaatgc accgcttaaa caaaagatga atgtcaacat
    83881 tgctccttca aagtaattac cgatcaaaca ggcaccaata gcagctaaag ccatcaataa
    83941 atccacattc agtgtccgat cattccacaa ttctttcaag ccttccgatg tttgtttata
    84001 tccaccgatg ataatggcac tgatataaaa aagtggggtg atggtaagag agaatcggtc
    84061 gaagataaaa ccgaagatca taaataaaag gcagccgatt gttgctgcaa aagctttttt
    84121 ctgattagtc atcgttatta gacctccttg tcaaaacaag gatagcataa taaaaatgta
    84181 attgataata ataacagcta aatgagaacg ataatggtta tcatttggtg attaagcttg
    84241 atgttgtttt tattatttat aatgattatt attaaagaaa ataaaaaacc agaacatttc
    84301 tcaaataaga
//
LOCUS       DAYYFU010000007        74845 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000007 DAYYFU010000000
VERSION     DAYYFU010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 74845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.007"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(552..2165)
                     /locus_tag="ACHA34_000587"
     CDS             complement(552..2165)
                     /locus_tag="ACHA34_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8791262.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2306..3214)
                     /locus_tag="ACHA34_000588"
     CDS             complement(2306..3214)
                     /locus_tag="ACHA34_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8791263.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3397..4521
                     /locus_tag="ACHA34_000589"
     CDS             3397..4521
                     /locus_tag="ACHA34_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8791264.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4546..4725
                     /locus_tag="ACHA34_000590"
     CDS             4546..4725
                     /locus_tag="ACHA34_000590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791265.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            4748..5665
                     /locus_tag="ACHA34_000591"
     CDS             4748..5665
                     /locus_tag="ACHA34_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8791266.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5658..6491
                     /locus_tag="ACHA34_000592"
     CDS             5658..6491
                     /locus_tag="ACHA34_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8791267.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6574..7692
                     /locus_tag="ACHA34_000593"
     CDS             6574..7692
                     /locus_tag="ACHA34_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8791268.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7713..9584
                     /locus_tag="ACHA34_000594"
     CDS             7713..9584
                     /locus_tag="ACHA34_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFM8791269.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            9578..10801
                     /locus_tag="ACHA34_000595"
     CDS             9578..10801
                     /locus_tag="ACHA34_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8791270.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10887..12821)
                     /locus_tag="ACHA34_000596"
     CDS             complement(10887..12821)
                     /locus_tag="ACHA34_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8791271.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13047..13703
                     /locus_tag="ACHA34_000597"
     CDS             13047..13703
                     /locus_tag="ACHA34_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8791272.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13777..14130)
                     /locus_tag="ACHA34_000598"
     CDS             complement(13777..14130)
                     /locus_tag="ACHA34_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8791273.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14322..15251
                     /locus_tag="ACHA34_000599"
     CDS             14322..15251
                     /locus_tag="ACHA34_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFM8791274.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15262..16098
                     /locus_tag="ACHA34_000600"
     CDS             15262..16098
                     /locus_tag="ACHA34_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFM8791275.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16346..17122
                     /locus_tag="ACHA34_000601"
     CDS             16346..17122
                     /locus_tag="ACHA34_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8791276.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17298..18041
                     /locus_tag="ACHA34_000602"
     CDS             17298..18041
                     /locus_tag="ACHA34_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8791277.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18034..19614
                     /locus_tag="ACHA34_000603"
     CDS             18034..19614
                     /locus_tag="ACHA34_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFM8791278.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19865..20344)
                     /locus_tag="ACHA34_000604"
     CDS             complement(19865..20344)
                     /locus_tag="ACHA34_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8791279.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20360..21112)
                     /locus_tag="ACHA34_000605"
     CDS             complement(20360..21112)
                     /locus_tag="ACHA34_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8791280.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21229..21299
                     /locus_tag="ACHA34_000606"
     tRNA            21229..21299
                     /locus_tag="ACHA34_000606"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21261..21263,aa:Gly,seq:ccc)
     gene            21660..22385
                     /locus_tag="ACHA34_000607"
     CDS             21660..22385
                     /locus_tag="ACHA34_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM8791281.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22640..22906
                     /locus_tag="ACHA34_000608"
     CDS             22640..22906
                     /locus_tag="ACHA34_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFM8791282.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22903..23334
                     /gene="ruvX"
                     /locus_tag="ACHA34_000609"
     CDS             22903..23334
                     /gene="ruvX"
                     /locus_tag="ACHA34_000609"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFM8791283.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23359..23664
                     /locus_tag="ACHA34_000610"
     CDS             23359..23664
                     /locus_tag="ACHA34_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFM8791284.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            24233..25070
                     /locus_tag="ACHA34_000611"
                     /pseudo
     CDS             24233..25070
                     /locus_tag="ACHA34_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            25135..25569
                     /locus_tag="ACHA34_000612"
     CDS             25135..25569
                     /locus_tag="ACHA34_000612"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8791285.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(25637..26959)
                     /locus_tag="ACHA34_000613"
     CDS             complement(25637..26959)
                     /locus_tag="ACHA34_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFM8791286.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(27113..28753)
                     /locus_tag="ACHA34_000614"
     CDS             complement(27113..28753)
                     /locus_tag="ACHA34_000614"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFM8791287.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            29083..29553
                     /locus_tag="ACHA34_000615"
     CDS             29083..29553
                     /locus_tag="ACHA34_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFM8791288.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            30182..31285
                     /locus_tag="ACHA34_000616"
     CDS             30182..31285
                     /locus_tag="ACHA34_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFM8791289.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            31569..32249
                     /locus_tag="ACHA34_000617"
     CDS             31569..32249
                     /locus_tag="ACHA34_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8791290.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            32339..33373
                     /locus_tag="ACHA34_000618"
     CDS             32339..33373
                     /locus_tag="ACHA34_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8791291.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            33387..33722
                     /locus_tag="ACHA34_000619"
     CDS             33387..33722
                     /locus_tag="ACHA34_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791292.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            33706..36186
                     /locus_tag="ACHA34_000620"
     CDS             33706..36186
                     /locus_tag="ACHA34_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791293.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            36242..37801
                     /locus_tag="ACHA34_000621"
     CDS             36242..37801
                     /locus_tag="ACHA34_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8791294.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            37798..39162
                     /locus_tag="ACHA34_000622"
     CDS             37798..39162
                     /locus_tag="ACHA34_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8791295.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            39333..40403
                     /locus_tag="ACHA34_000623"
     CDS             39333..40403
                     /locus_tag="ACHA34_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8791296.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            40418..41086
                     /locus_tag="ACHA34_000624"
     CDS             40418..41086
                     /locus_tag="ACHA34_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8791297.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            41106..41684
                     /locus_tag="ACHA34_000625"
     CDS             41106..41684
                     /locus_tag="ACHA34_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8791298.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            42087..42998
                     /locus_tag="ACHA34_000626"
     CDS             42087..42998
                     /locus_tag="ACHA34_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8791299.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(43254..44171)
                     /gene="rnhC"
                     /locus_tag="ACHA34_000627"
     CDS             complement(43254..44171)
                     /gene="rnhC"
                     /locus_tag="ACHA34_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFM8791300.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            44373..44810
                     /gene="zapA"
                     /locus_tag="ACHA34_000628"
     CDS             44373..44810
                     /gene="zapA"
                     /locus_tag="ACHA34_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFM8791301.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            44868..45415
                     /locus_tag="ACHA34_000629"
                     /pseudo
     CDS             44868..45415
                     /locus_tag="ACHA34_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            45530..47890
                     /locus_tag="ACHA34_000630"
     CDS             45530..47890
                     /locus_tag="ACHA34_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM8791302.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            47977..48291
                     /gene="trxA"
                     /locus_tag="ACHA34_000631"
     CDS             47977..48291
                     /gene="trxA"
                     /locus_tag="ACHA34_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFM8791303.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            48473..50251
                     /gene="uvrC"
                     /locus_tag="ACHA34_000632"
     CDS             48473..50251
                     /gene="uvrC"
                     /locus_tag="ACHA34_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFM8791304.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            50396..51295
                     /locus_tag="ACHA34_000633"
     CDS             50396..51295
                     /locus_tag="ACHA34_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8791305.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            51288..52550
                     /locus_tag="ACHA34_000634"
     CDS             51288..52550
                     /locus_tag="ACHA34_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8791306.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            52713..54611
                     /locus_tag="ACHA34_000635"
     CDS             52713..54611
                     /locus_tag="ACHA34_000635"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFM8791307.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(54910..55629)
                     /locus_tag="ACHA34_000636"
     CDS             complement(54910..55629)
                     /locus_tag="ACHA34_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFM8791308.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    55819..55866
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            55966..56298
                     /locus_tag="ACHA34_000637"
     CDS             55966..56298
                     /locus_tag="ACHA34_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791309.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            56285..58288
                     /locus_tag="ACHA34_000638"
     CDS             56285..58288
                     /locus_tag="ACHA34_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFM8791310.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            58307..58777
                     /locus_tag="ACHA34_000639"
     CDS             58307..58777
                     /locus_tag="ACHA34_000639"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFM8791311.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            58798..59382
                     /locus_tag="ACHA34_000640"
     CDS             58798..59382
                     /locus_tag="ACHA34_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFM8791312.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            59397..60383
                     /locus_tag="ACHA34_000641"
     CDS             59397..60383
                     /locus_tag="ACHA34_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFM8791313.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            60373..60684
                     /locus_tag="ACHA34_000642"
     CDS             60373..60684
                     /locus_tag="ACHA34_000642"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFM8791314.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            60706..62487
                     /locus_tag="ACHA34_000643"
     CDS             60706..62487
                     /locus_tag="ACHA34_000643"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFM8791315.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            62480..63856
                     /locus_tag="ACHA34_000644"
     CDS             62480..63856
                     /locus_tag="ACHA34_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFM8791316.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            63863..64498
                     /locus_tag="ACHA34_000645"
     CDS             63863..64498
                     /locus_tag="ACHA34_000645"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFM8791317.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            64528..65883
                     /locus_tag="ACHA34_000646"
     CDS             64528..65883
                     /locus_tag="ACHA34_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8791318.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(66075..67469)
                     /locus_tag="ACHA34_000647"
     CDS             complement(66075..67469)
                     /locus_tag="ACHA34_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8791319.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            67561..68532
                     /locus_tag="ACHA34_000648"
     CDS             67561..68532
                     /locus_tag="ACHA34_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM8791320.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            68529..69311
                     /locus_tag="ACHA34_000649"
     CDS             68529..69311
                     /locus_tag="ACHA34_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFM8791321.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(69480..69851)
                     /locus_tag="ACHA34_000650"
     CDS             complement(69480..69851)
                     /locus_tag="ACHA34_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8791322.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            69985..70428
                     /locus_tag="ACHA34_000651"
     CDS             69985..70428
                     /locus_tag="ACHA34_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8791323.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(70471..71280)
                     /gene="yidA"
                     /locus_tag="ACHA34_000652"
     CDS             complement(70471..71280)
                     /gene="yidA"
                     /locus_tag="ACHA34_000652"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFM8791324.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(71299..72666)
                     /locus_tag="ACHA34_000653"
     CDS             complement(71299..72666)
                     /locus_tag="ACHA34_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8791325.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(72650..73480)
                     /locus_tag="ACHA34_000654"
     CDS             complement(72650..73480)
                     /locus_tag="ACHA34_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFM8791326.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            73643..74080
                     /locus_tag="ACHA34_000655"
     CDS             73643..74080
                     /locus_tag="ACHA34_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFM8791327.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            74156..74776
                     /gene="rpoE"
                     /locus_tag="ACHA34_000656"
     CDS             74156..74776
                     /gene="rpoE"
                     /locus_tag="ACHA34_000656"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFM8791328.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        24419 a        12819 c        15418 g        22189 t
ORIGIN      
        1 ttaaataaat aggaacaaaa aaattaaatt atttaataca cacattaggg atcattcatt
       61 gaaggattag aagacaacga ggatggcgaa aaatatgatt acaatagaaa agtcgttcta
      121 aaccaatcag ttcctagtat cacacaacgc aagccagtcg atttggtgag attagaggag
      181 tgaaagcaaa ctttattata taagaaaaaa tattcgaaag ggatgctagt ttaggtacta
      241 gtgctgatag attaatatga tgcctcgtaa tattaggcgt agttactcta gagacatcac
      301 tatggactat gatttgtctc agagtcacaa catgtttttt tatgaaaaga aacagctaaa
      361 ataattaaat tgcgcaatgt ttaaactttc ttaaactata acctaataaa tgtgaagaaa
      421 taatgcacaa tttcttcaca tttatcttct attctatata cataccaacc aacaacccta
      481 tatacttttt catttttact cctcaagtaa aaataactcc tagaccgcgg ctacccaccg
      541 cggtcgtttt tttattcttc aatcaaaatc gctttgatac ccaaacctgc caagattcgg
      601 gcagaagtat aagtattcaa tcctcttctg aaagtaatgt aaactttttt atcttttggg
      661 atttcattga ttcgatctcg taactcattc attggaatat tttttgttgc cgagatactg
      721 ccagaaggtg ctttattagg ttcacgtata tctaaaaaga acgcttcttt caaatcgttt
      781 tcgggtatat cacttgcttt gatcgtttct acgatattcg tcatttgatt gattgcgaca
      841 taaccagcta tattcaacgg gtcacgggta gtagaatacg gtggagagta aggcaactcc
      901 aaatcaggca aatcaaagac tgtcaagttt ccggtgattg ctacagaaag ttctcccatc
      961 cgtttatcga cacctttttc tccaaatgct tgtccaccta aaatacgacc gctatctgca
     1021 tcaaaaatca gctttagatt taatcttgta gcttctggat agaaataggc atggtcaaag
     1081 ggagtgataa aaacagtttt ataatttgtt atgcctgatg cttttaattc atgttctgtc
     1141 attcccgcat aacttgctgt atgatcaaag atttttgcga cacctgcacc aatatatccg
     1201 cgatagcgca taggcgtacc atttaaaata tctgccagca tatgtccttg acgattggcc
     1261 gcactggaaa gcatactttg gatcggctga ccagtcacta cactagtcgt ttcgataata
     1321 tcgccaatcg catagatgtc cggcagattg gtttgtaact ggtcatcaac aatgatctga
     1381 cctgtatctg ataattgtat gccggttgct ttcaccactt cattcttcgg agaaacacca
     1441 acagcaaaaa ttagcatatc agcagaaagg acggagccat ctgataatac tatttctcga
     1501 cctttatcac ggatctcagt aactcttgtt tctaaatgaa cggctaatcc ttctttcagc
     1561 aatttgtcat aaactaaatt agcaatttct ggatcataag ggaaagcgac ttgtggcaat
     1621 tgatcgatta aagtcacgtc taagccgcga tgcttgaaat tctcagctaa ttccagaccc
     1681 ataacacctg caccaagaat gacgacatgt tgtggatttt tttcttcaag gaacgatttg
     1741 attcgatccg catccgtcac actacggagg acaaagccgt tttctgcttc aaaaactccg
     1801 ggaatatccg gataatctgg tctagcacca gaagagatga tcaacttatc gtaatctgtt
     1861 tttatttctt catttgtgga taggtcttta actgttaatc gcttagtaga aggatcgatt
     1921 gctgttactt cgtgtttagt aaagacatcg atgttgtttt ttgttttcaa tatttctggt
     1981 gtacgttcga tcagcgagtc acggtcagtg atcacacctc ctaaataata aggcaaagca
     2041 caacttgcat aagaaatatt ttctccgcgt tcatagataa tgatttcgtg cgcttcattc
     2101 aatcgacgga aacgagtggc aaatgaagga ccgccagcta caccgccaat gacaacaact
     2161 ttcataaaaa catctccttt tctacgttga tcgtactatg ttatggtaaa aaacagcaaa
     2221 taattgtgcc ggaaaattat tgtttatttt gaatagacag aaaatataga gaaataattt
     2281 gtggataaaa cctaaaaatt tttttctatt ttaaggatag ataatacttt gaaggggata
     2341 accctgtata ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat
     2401 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
     2461 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
     2521 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
     2581 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
     2641 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
     2701 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
     2761 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
     2821 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
     2881 taatgataga tgttatttct tgatctatag aaaaagattg tcccgtaata tgtgctaata
     2941 attgctcatc aaaataaaac tggataatga taaaagtttg tgtttccttt tgagtagaaa
     3001 agatttggac ttcttgctgt ttatcaatga ataaaaggga aggattatct agttctatag
     3061 atttatgtgc aaattcagca tataaattag cgctttcatt tattagtagt cgaaaatagg
     3121 gagcagcaga taaatgaaga tgggtatcta tggaataggt tagtgttttt ttgacaagat
     3181 tggaggattg gttgtttgtt aacaaaagaa ccactttcct ttctcttttt atataaaaag
     3241 ctttctattt ataattaatg actattataa caaaaatgaa aagccaaaac gacaagtaaa
     3301 atattttttt atataaatat ttgtgttact aattccgtta atatacataa gtgaaagcgt
     3361 tttatataag aacttgaaaa gaaagaaggg atagacatga aaaaaagata caaattggtg
     3421 atagctttgc taaacattac cctattattc agcttaagtg catgtggaaa aactaacgaa
     3481 gaagtaacag caaacatcgc agatccaatg ataaaaaaag ccactacatt agaagtatat
     3541 gtcgcaaacg gcaaactgac aaactatgtt gaaaatgctg caaaaatcta taaccgcaaa
     3601 actggagcaa atattgactt aaaaattgtg aatgtagctt cgggaacagc aacagtacaa
     3661 atgatcaccc caaaattggt aacacacgaa gatatgccgg atattatctc catggcagac
     3721 acttcagcag caggtgtgct ggaaaaattt caagatgctt tttatagtgc tgatgaatat
     3781 ggattttatg acaaatatgg cgatcagttt tatgatcaaa aattgaatat tttgcgtcaa
     3841 caaacgagta atgaaacgat tattccttgg gctagtgatt ttaccccagc actttgttat
     3901 tatcaaccaa aaaaatttgc tgaaataggt gtagattttg aagaaatcag ttcttgggaa
     3961 gaatatatcc aggtggcaaa agcattaaaa gaaaaaactg gtagttttgg aattgccttg
     4021 ccagaatctg gggaccaaga attgtttatc aacatgctag cccagcagaa tcaatctttg
     4081 cttgatgaag aaggaaatat ccgtctgaat actaaagaag cgaaacatgc tgcaaagtta
     4141 atcaaagaaa tgatcgactc agatattgta catttctacg gtgcacaaga tgctgagaaa
     4201 gcatttcaag aatctgcaat gtttgttgct ggcggctggt atgctacgaa tatgtctttg
     4261 aattttcctg atgcatctga caaatggcgc atggctccac tgattccttt ttcaaaagaa
     4321 aatccaggaa aaggtgcagt atcaggagga tcaagttttt atgtccctaa agatgccaaa
     4381 aatccacaag tggcacaaca atttcttaca tttatgctga cagatgaaga atgcttagcc
     4441 aacgcactgg agctgggcgt ggctacatct aactcattgg cctatcaaac agaagcagct
     4501 gagaaaaagt ttgactacta aggaggacaa aaatactatg aactattgaa taaaatcaat
     4561 gaaaatacat caaatactgt tttaccagcc acttattcag atgctacgtc ttatttgact
     4621 accgctgcat ataactattg gcaaacaggt gattttgaaa attcatatct aaaagaagca
     4681 gaaaattttg ctcataagta cggcgtaaaa ttaactggtg attaaaaaat attaggagga
     4741 cttaacgatg atgcaatctt cgttaccaag caagacaata aagaacagca agaaaaaagc
     4801 aacagatacg tctcataaaa aaagtgctta tggattggtc acaccttttg ttattttctt
     4861 tattgtgacg attttgattc ccatcattat ctcgtttgct tttagtttta tgaaaattgg
     4921 ctactcattt gaatttgttg gatttgacaa ttatctagct atcttccgag atccattgtt
     4981 tattaaatct tacacgaatg ttttgattat catggcagga tcaattccaa taacgatgct
     5041 attagctatt tactttgctg ttttattgaa ttcagcacag ttaaaaggaa aaggattttt
     5101 tagaactgtc tattatattc caaccattac atcaacggtg gctgtagctt ccgtattcat
     5161 gacttttttc aatccgactg ggttattcaa tggatttttg aatgcgctag gaattaacgg
     5221 tgtacagtgg ttaacaaatc ctttttggat acgaatatca atgatcatca cgatgatttg
     5281 gcttagtgta ggatataaca ctattttatt tttagctggt cttcaaggga tttctaaaga
     5341 gatatacgaa agtgctgaaa tagatggagc atcgaaattt cggcaattct ttttattaac
     5401 gatccccatg ctaaagccaa tcattttaat ggccacagtt ttagccacta tcaatggctt
     5461 gggtaatttc aatatcccaa atatattctt tgggacttcc aatggaccag aaaattcagc
     5521 tttggtcgta ggagtcaatt tatataaaac aagttttgaa atggttgatt ttggaaaagc
     5581 ctctgctatt gcctggacga tggtgtttgt atctgttata ttatcggcta ttcaatttaa
     5641 attcggaggt gataaaaatg actaaacaaa agatacaaag cagaattttt acgtacgtca
     5701 ttattgtgtt tggtgcctta atatttttga tgccatattt atatatgatt gtagcttcaa
     5761 cgcaaagtaa tcaagaaatt ttaagtggcg gaatcaattt caagtttggg gatatgctat
     5821 taaaaaactg gcaagccatc atggcgagat ataattatcc cttgattatt tggaattcga
     5881 ttgttattgc tgtaatcagt acagttttag ctacagccag ttctacaatg gcgggttatg
     5941 cattagcaaa gtatcgtttt atggggaata aactgttgtt caatacagtt atgctatcta
     6001 ggatggtgcc tttttttacc acattgattc ctttatttta cattatgagt cgtttaggat
     6061 tatcgaatac ttatgcaggt gtgatcatcc cttcgattgc cagtacaact tcagtgttta
     6121 tgatgcgaca atatagcagt caatttccta cagcactgat ggaggctgcc agaatcgatg
     6181 gtgccaatga gtgggtgatt tttacgaaaa tcgctgtacc gattttatca cctacgatta
     6241 ttactagtgg tttacttatc tttatgggaa gttggaatca atatctattt ccattagtca
     6301 tgctttcaga taaagataaa tatacggttc ccttaattat caaaaatatg acggtttcta
     6361 gtacaggtga gccaattaat tatggcgcaa ttatgctaat tttagcaact tcagtgatcc
     6421 caatggtctt gatttatgtg tgggcgcagt ctaaatttaa ggagaatgac attggagctg
     6481 caaataagta aaaaaaataa cgaatgttaa ccatctagtt cgttctagga gcaactccac
     6541 attcataaaa attaaaagaa tggcgggata tccatgaatg aacagacgaa ggaatggtca
     6601 agaaaaattt tgaatgtagc ttctgaaaag atagatgcgg aattagacag aatggggaat
     6661 aaaattccat atatttctta caacggaaaa tatacagacg taataaagga cagaggaatc
     6721 gactggtgga ccaatggttt ttggagtggt attttatggc agctctttca caagacaaaa
     6781 cgagaaaagt tcaaagaggc agcaatacaa caagaacaat tattagaatc tgccctgtac
     6841 catttttcag acgttcatca tgatgtaggt tttttattct taccatcggc tattgcacat
     6901 tatcaacagt ctcaaaacaa acatagtcga caactagggt tacatgcagc tacggtactt
     6961 gctggacgct ataatatttc agggaactat ttagtagcat ggaatgacca taaacctggt
     7021 tggatgatta ttgatagtat gatgaattta ccattactgt attgggcttc gagggaaact
     7081 ggtgatccga ggttctctaa aattgctcgg cagcatgcag atacaacagc taagtatcta
     7141 gtgaggaaag atggatcaac cgggcatatc gcagaatttg atgcagaatc agggaaatat
     7201 attcggttac ttggcgggca aggatacaat gagaattctt gttggagcag aggaaatgca
     7261 tgggctttat atggattcgc gttaagttat cgttacacaa aaaatccgta ctatctagct
     7321 atagcagaaa agatagcagt aaattttatt cagaaaatcg aaaaaactgg ttatattcct
     7381 ctcattgatt ttgctgtaaa agattcgaat attgatacca gtgctggagt atgcgctgca
     7441 tgcggtcttc tggaattagc aggatatgtt tcctctgtac gatcaaaaat gtatcatgaa
     7501 gctgctatca atatactcca aaagacagaa aagcactatg caaattggaa tatggatgaa
     7561 gatgggatca ttggaggcgg agcagaagca taccatagag aactgactta taatgtgcca
     7621 ctgatctatt ctgattattt ttttattgaa ggcttacttc gattagaaca gtcggaatta
     7681 gctatttggt agtaaaaaga ggagaaaata caatgaaaca tccagtacca ctattaagga
     7741 ccagacagga ttactttgac ttatttaaaa agataaatca accactccag ccgttttatc
     7801 gagcaagtgg cgcacaaatt gattatatac atcaaggggt aggatatgga aataaaattg
     7861 caggaatgga aggattctct cgggttttat ggggagcggg gcccgcaatt gaccaattag
     7921 atgaacgatg gttgaaatta attctatcag gaataaaagc tggaacagat ccttcccatc
     7981 ccgattattg gggagaaata catgaaagag accagcgtat ggtagaaatg cccgcaatgt
     8041 tattagcgtt atatcatcat gaacaattat tatggaaaaa attagataca ggagaaaaaa
     8101 gccagattat tcattggttc tcgcaaattt ttgaatatga atgtgcagat ggtaattggc
     8161 aattttttaa gataattgtc ggaacaatta tccaaaaatt tggttatcca gttaaagaat
     8221 cagcactgaa agaagcattt gataatatag aaaattgtta tcttcaaaat ggctggtatc
     8281 gagacagttc aagaggtcgc caagattatt ataatccttt tgcatttcat tattatggtt
     8341 tgatttatag cgtactagaa ccagaaaagc ccattagcca gatatataag aaccgagcta
     8401 ttgatttttc tagagactat attcattttt ttgcggaaga tggtgcaaat gtgccttttg
     8461 gtcgaagcat gatttatcgt tttgcagttt caagcttttg gtcagctatg ttgtgggccg
     8521 atcttttgcc ttttaaatta ggtgaaatca aggggttagt caatcggaat ataagatggt
     8581 ggatgcaaaa agaaatattt gatgaaaaag gtatcctgaa tattggttat acctaccctc
     8641 aattgacatt gactgaaccc tataatagta cgttatctcc attatggctg aataagattt
     8701 ttttattgtt ggcgctacct gaagaccatg agtattggac ggctaaagaa atgcctatgc
     8761 ctattcgtga aggaactaaa cttctttcag aagccaattt gctcatacaa catgataatg
     8821 gacatacatt ttttttgaat gcgggacaat tagggccaaa tttccataca ttgaccaatg
     8881 aaaaatattt aaaatttgct tattctagtc aatttggttt ttcgataccg cgagcgaatc
     8941 aattgaaagc agaaatggca atggattcaa tgctagggat tcagcgagcg gacacatata
     9001 tcacgaccag ttttaagaat gagagcatca aaacctatgg acagttcatc gttcgaaata
     9061 atgtacgaga cattgtatgt aagaaagagg tacttgcctc tacatgggaa cctactttac
     9121 aggcaaaaat cagaacttgg cttattcctt ttgaaggctg gcagattcgc gtacacaaag
     9181 tagaattaga tacagaatat gttctatatg agacagggtt tgctattgaa tgttctcctg
     9241 agaaagaagg aataatcata gaagaagaca aggaaaacta tagggtgagt gaagctggct
     9301 tttcaggtat tatctgttta agtgacgata ctattaaacg aaaatccaca gctattatgg
     9361 gtttgcccaa tacaaacttg atgacatggg aaaacacttt tattcctgga ttagagggta
     9421 catttggaaa aggaacccat tggctcggaa caggagtggt tgctcatcaa gatcgggatt
     9481 atagtttaga agtatggaac aatcgtccca aaattgattt tgatgggaca accgggctaa
     9541 ctatccaaaa taaaaacaat aaaaaaagac taaaattatg ctaaaggctg gtggggaaat
     9601 gcttgataaa gtgatgtttg aagagataat acatccagaa aaatggataa atgaatgtta
     9661 taaagaatta atggaaaaag aatggaataa tgagatcgag cgttgtcgca gacacacgga
     9721 gaggttaatc aaacgtatca gtaacagtca gtatcaattc ccaagcgagt gtcctataaa
     9781 aggacgttat ccattaagag acaatattgg ttggacagaa ggattttgga caggaattaa
     9841 ttggttgtta tttgagaaga cgggtgatcc tttatttcaa caaacagctg aaaaagcaga
     9901 tatagatttt atccacagga ttgactgtca aattatcact aatcatcacg atcttggttt
     9961 tttgtattct ttatcaaccg tagcagatta taaaataacg ggaaatcgaa aaatgaagga
    10021 agcttcaatt gatgctgcag aaacattact tcagcgtttt tgggagaagg caggagttat
    10081 acaagcttgg ggagatccta gtgatccttc acaacaaggt agaatgatca tggattgttg
    10141 catgaatctt ccattattgt attgggcgag tgaagaaact ggaaatcaaa aatataaaaa
    10201 agctgctcag gctcatattc aaaatgcagc agattattta gtaagagaag atggttcaac
    10261 ttatcataca ttttatatgg atgtcaaaga tggaaaacca agatatggaa gtacgcatca
    10321 aggttatagt gataattcct gttgggctag aggccaagca tgggggattt acggttttgc
    10381 attaagttat cgttatttga aagaacaaaa atattttgat ttaagcaaaa tattagccaa
    10441 ttattttatg aatcggttgc cagaagatac catttgttat tgggatttga tctttgatga
    10501 ttctagcaga gaagaaaaag atacatctag tatggtgatc atggtttgtg gattattaga
    10561 attaatagaa tcaggtctat tagacaaaac agaaaagaac ttataccagt ctgctgtttg
    10621 gaaaattatg cgacaattga aagaaagata tgctacaacg gaagaagaag atggcttttt
    10681 aacacagtct gtttataata agcccaaaaa taatggggta aatacacctt gtatttgggg
    10741 tgattactat tatttagaag ctttagtgag aatgactagt aattggtctt cttattggtg
    10801 aaaaaggtaa atatggaatt tttgtttgat aaaataaaaa aagaaaaacc aaaacagcat
    10861 gaatggtttc tgttttggtt ttttcgttaa ttttcatatt cttctaattc caagcttagt
    10921 tcttcccatt ccgtcaattt tgtttcttga tcttgtctag cagcttctaa atcttggttc
    10981 agctcattga gttttacgtg atcttccagg ttttctggcg cgaccatttc ttgttccaac
    11041 tgatcgatcc attcatcgat ccgcgccatt tcttcttcaa cctgagtaat ttttcgttga
    11101 aggctacgga taagcttttg ctgttctttg ctttggtaat aatcagattt gttcgtggga
    11161 gcaggagcag attctggctc atcttgtgcc aacaattcgg cgatttcttc ttcttcctgc
    11221 tttttctcta aataataatc atagtctcct agatagactt tgctgccatt ttcagagagt
    11281 tcgacgactt ttgtcgcgat ccgattgatg aaatatcggt catgggaaac aaaaagcagt
    11341 gtgccgtcat aatcgatcaa tgcattttct aatacttctt tgttgtcaat atctaaatgg
    11401 tttgttggtt catccaaaat caaaaaattt tctttgttca tcgctaactt ggctaaagca
    11461 acccgtgctt tttcaccgcc acttagcaga gggattggtt tttctacatc ttcgccagaa
    11521 aataagaaag cacctaaagc acttcggatc tctttttctg gagtggtagg atgctcatcc
    11581 cacaattctg ctaggatcgt cttgtttgta tgcagatcag cttgaccttg atcgtaatag
    11641 ccgatggtga catttgtccc tagcgtttct tttccacgga taaaagggat ctggtgaatg
    11701 attgatttca gcaacgtcga tttcccgata ccattcggac ctactagagc aatcgcttct
    11761 tgacgacgga tatctagatt gattggttca gataaggtga cattatcata tccgatagcg
    11821 gcatcttcga tttgcaagac tacatttcca gaaggctttt ctatactgaa tagaaagttt
    11881 gcttgttttt catccccttg aggacgatcc agccggtcca ttttttctaa tgtctttctg
    11941 cggctttgcg ctcgttttgt ggtagaggcg cgaacaaggt tccgagcaac gaagtcttct
    12001 aatttatgga tttctgtttg ctgcttttca taagccttcc attcacttgc taactgttct
    12061 gctttcaaat ctagatattt cgaatagtta cctttgtaat ggcgcatttt tcttctgctt
    12121 aattcataga cttcattgac tactttatct aaaaaataac ggtcatggga aacaatcaat
    12181 aatgcaccag tatagctttg cagataattc tcaagccagg aaagtgtctc gatatctaaa
    12241 tggttcgttg gttcatctag aatcaaaatg tcagggtgtt gcaaaagcat gcgagctaaa
    12301 gctaaacgtg tgcgctgtcc gcctgataaa gtatcgattt tctgatcata aaatgaagaa
    12361 tcgaatttaa acccatgcaa tacggaacga atttcatttt cataaccata gccgtttcga
    12421 tctgaaaaat cgtgttgcag ccggtcgtat tcttttaaat aagaactata agcaggtgtg
    12481 tctggcgtac cttcgctaat gaaaatctct agctcgcgca tccgctcttc catttgtcta
    12541 acttgttcaa aagctttcag catctcgttc caaactgttt cattggaatc aagtcctgtg
    12601 tcctgagcga ggtatccgag agtcgctgtt ttatttttag cgatctttcc ttcatcaggt
    12661 gcttcaattc cagcaatgat ctttaataaa gtcgattttc cagcgccatt tctgccaact
    12721 aaagcgacac gagcgcctgt actgatttct aaatgtatgt tttcaaataa gacttctgca
    12781 ccaaaatggc gagcaatctg attggcttgt aataatatca tggaatcctc ttttctactt
    12841 tgtgaattct cctcaaataa aaaactattt ttcaattagt agtctaccat aaattccatt
    12901 atactgcgag atttgcagaa atgataaata aatgaataaa gaataaaatt tcacaatatc
    12961 tgagcaatgg aattgatttt ttcctataaa tactgctatg atggagaaat caaagatgta
    13021 attagacaat acgtggaggt catttcgtga aagatcatat tattccaaag gcgacagcta
    13081 aaagactgcc tttgtattat cgctatttaa ggattttaaa taatgctgga aagaaaaaag
    13141 tgtcttcaac tgaattaagt gaagccgttc aagtagatag tgcaacgatc cgtcgagatt
    13201 tttcttattt tggcgaatta ggaaaacgtg ggtatggtta tgatgtagaa gatcttatgc
    13261 acttttttgc aaagacatta aacgacgatc aattgacaaa tgttgcgctg atcggtgtag
    13321 ggaacttggg aagtgcttta ctaaaataca aattccatca aagtaatagc atacgtatca
    13381 gttgtgcttt tgatgtgaat gaggaaatcg ttgggcgcat cgtagacggt atccccgttt
    13441 atccaatgga agatatgtca gaacaaatca agatccaaca aatcgaagta gcaattttaa
    13501 cgatacccgc tgaaagagca caagaagtag tcaatcagtt aacggaagca ggaatcaaag
    13561 gaatcttgaa ctttaccgct gctcgattga atgctccgtc agatgtattg atccaaaatg
    13621 ttgatttgac gaatgaattg caaaccttga tctatttctt gcattctgac gcaaatgcgc
    13681 attccgaaaa tacagcagaa taaaaataaa aagcgattga acaaatgaag gcaaaacttt
    13741 cacttgttca atcgcttttt tctgtttgga caacgattat tttgttcgga agatttcttc
    13801 ataagaaagg gaatcatctt ctgctgtcag ttcatctagt agataacgga tttttttaaa
    13861 gtcgatgaac cgtgcttgcc gaaagacttc ttttccttga tgaaaaatca ggacaactgg
    13921 cgcagtcagc acttcaaatt tactagctac ttctggtgtt tcaaatgcat ccaaatgaag
    13981 tgccggaata tcataatgtg tgagaagttc ctctagtcgt ggacgaacag cgtgacaaac
    14041 actgcaatta ggcatcgaaa gataaatgac ggcaacggat gactgttgca gtatgtcttg
    14101 aacttcagtc aagttagtgg tttgtttcat ttttattctc ccttttatga tagaagtgat
    14161 atgtatgaga ccttaattta atgggaataa cagaaaaagt caattgaagt gctttggtaa
    14221 aaaataagag ttgtgtaaaa atttacagag caacttgttt tttttgaata aaagcgtata
    14281 tttgcaaagc gaatgaacta gaaataaaaa ggaggaaact catgttttct tttattccaa
    14341 attcggtttt gcagatgagt acgattttct tatcgatcgt tattgaagca ttacctttcg
    14401 tattattagg ctgtcttatc tcaggcgcct tacaagtatt tttgacccca gaacgtgtga
    14461 agagatggct tcctgaaaat cgtatcttct ctatttttac tggttcgatc ttaggatttt
    14521 tctttccttc atgtgaatgc ggcatcgtac cgatcgttca tcaatttgtt aaaaaaggcg
    14581 taccagttca cacagcgttt gcttttatgc tgactgctcc gatcatcaat ccaatcgtga
    14641 ttttttccac ttttatcgca tttggcaatt cttggcagat ggcaagctgg cgggtgatag
    14701 gcagcttttt tgttgccctt gtgattggtg tctggcttgc ctattttcaa aaagacgctg
    14761 tattgaaaga aaaaatcacg tctaaagtag cagaaaatca ccatcatcat gtccatgaag
    14821 aagaacaaag tgagaagaat gaaccaaaaa atcagctgat ccatcaaact gggcatgtgt
    14881 tgacacattc aatcgacgaa ttttttgata caggtcgtta tttgatcatt ggcggattaa
    14941 tcgcagctgc gatgcagact tatttaccaa ctagaattat gctaacgctc ggctcaacaa
    15001 agattttagc cattatcatc atgctgttat tggcctttac gatgtctctt tgctcagaag
    15061 cagatgcgtt tataggatct tctttgttaa gtctgttcgg cacagcacct gtagctgcct
    15121 ttttggtctt tggcccaatg gtcgatatca aaaatctact gatgatgaaa cgatatttta
    15181 caggacgctt tattttatcg ctgatcggac tgattgccct tagcgttatt ggattcacat
    15241 tatttattta ggagggaaaa tatgctgcgg tttttgattt tatctgggta tttcgaattg
    15301 atgatgtact tgcaagtttc tggcaaactc gatcaatata tcaatgtcca ttatcgctac
    15361 ttagctattc tttcaatgat tttatctgct cttttggctt tagttcagct gtatttgtgg
    15421 gtcaaaaacg gaaaagcaga agaaaaggca cacgagcacg atcatgacca tgggttgtca
    15481 aaaccttatc aacgtggaat cgcttatgta ctgctagctt tgccagtaat cgtggggacg
    15541 ttgtttccaa cagttagtct cgacactacg atcgtagaag ccaaaggatt taatttccca
    15601 gtaagtaaag aatcagtagg ggatccggag atgcagacac aatacttaaa gccggatacc
    15661 agtatttatt tcaacaaatc agactactta gaccaaatga atgagttgaa agaaaagtat
    15721 ggggataagc aaactatcaa gatcacagat gaaaactatt tagaagttat ggagctaatt
    15781 tataattacc ctagcgaatt cattggcaaa aaaatcagct atgaaggatt tgtttaccaa
    15841 acaccgaacg atagcaatac agatatcttc ttgttccgat tcggcattat ccattgtgtg
    15901 gcagattcag gagtctttgg tttgttgacg catatgccgg aaggagtaac ggttaaaaat
    15961 gatgaatggt ataaaataga aggaaccatc caatcagact attatcaacc attcaagcgt
    16021 gaaatcccat ctgtagttgt tacaaacgct gagaaagtct ctgctccgaa aaatcagtat
    16081 gtgtatcgtt ctttttaaaa gaacaaaata aataaacata gctcagacag ctgacacgca
    16141 aggacgtcgg ttgtctggct ttttttgcat gatgtattca tttgtttgat atttatttta
    16201 gaagcagcat cctgaaagta tttgatatcg gtttcaaagg tgttggaaaa gattgtgaag
    16261 agaattacaa tttattccta acatgtatgt tgaaaagttg tatgaaatga ttttgatagt
    16321 aaaatatcga gtggaggtac tttttatggt aacgcgtata tttattttgg atgatagttt
    16381 ggtttttgaa aaaatgctag tagaaatttt ggaagaatcc agaattttat taagtccttg
    16441 gaaatttgag atcgttcagg gattgaacgt tatacaattt gttgaattca ttaaaaataa
    16501 tgaaatctat tcctcagata tttttttcct agacatcgat ttgaacgcct attatagcgg
    16561 tatcgaacta ggcgaaatgc ttagagaaaa gaatgaaaag tgtaaaattg tttatctaac
    16621 gtctcatccg aataaagcaa taactgttat taatcgaaag attacgccta gtggttattt
    16681 gataaaagaa ttcacttatg aaaaaaataa aaaaagcatc caaagttact tattgaaaga
    16741 aaaagaaaac tatcctgttc aacatgctac gaaaaaaagg tggacaactg tccgttatgg
    16801 taatagcgat cactatatcc attacgatga aatcctttat ctaatgagca tgccaggtta
    16861 taaaaataaa ataggcctag tattgaaaga tgaagagatt ctcgtaaacg gcacaattaa
    16921 aaaatataaa aaatcactcg aatatgagta tttatgtaaa gatttaaaag cttatatcat
    16981 taatactgaa aacattacat ctattagcag agtaaacgaa atagtaacat ttgcaaatgg
    17041 agaacaacta gagatgggaa agagaagtat tgataaacta aagaatttta tgcaagaaaa
    17101 gatagaaaat gaatcatttt aaagaaaaca aagactcata tcttacctgt tagaagagtg
    17161 ttttcctgta gaagagaaca acccaagtag cagcctagtc gtttctatcc ttttttgtta
    17221 tgcactcttt ttgacattta ctccaccaat aacgaagggt acaataaaaa gtaataacaa
    17281 acaaaaggag tacagaaatg attgaattca tcaatgtaag taaaagttac gggaaaaaac
    17341 aagcattaaa agacttgtct ctctcgataa gacaagggga aatttttgga tttttaggac
    17401 ataatggtgc cggaaaatca actacgatta aaagtttagt tagtattatt gaaccatcat
    17461 cgggaacgat tttagtagat ggaatgaaat tgacggaaaa tcgattgagt atcaaacaaa
    17521 aaatcggtta tgtgcctgat tccccagata tttttttaca attgacggct ggagaatatt
    17581 gggacttgat ttcagctgcc tatgaattag acacacaaaa aaaagaaaag cgtttagcag
    17641 aattgacagc gctttttgat atgtatagtc atcaaaatga aacaatcgca agtttttcac
    17701 atggaatgcg tcaaaaaacg attttgatcg gcacgttgct gccagatcca gatatttggg
    17761 tattggatga acctttgcaa ggacttgatc ctcaagcagc atttgatttg aaagaaatga
    17821 tgaaggctca tgccgcaaaa ggaaaaacag tcattttttc tacacatgta ttggataccg
    17881 cacaacagtt atgcgataaa ctggctattt tgaagaaagg tgaattgatt tatcaggggg
    17941 cagttagtga attattgaat ggctcacctg atgaaacgct agaaaagatt tatttgaaaa
    18001 tggctggaag gcaagcgaca ggagatggac cgtatgtata agcgacaatt attagaatta
    18061 attcgagtta atcttttata tgttaatcca cagctcacga ataaagcaag agagaaagga
    18121 aaaaagggaa agcgattaac tatctatctt ttgtctcaaa atcttttttc agggctgatt
    18181 tttttactga tttacggatt tgctatgttt gctatgaact ttgttaaatt gccaggtttc
    18241 tttacttatt acgtggcact ttttggtatg ttaggtttaa ctcaaagtat atcagtcatt
    18301 tataatatct tttttgaagg aaatgacctg caagtatttt tgcctctgcc ttttggacaa
    18361 gaacaggttt tttcagcaaa gattttggta gttgctttga ccatcattcc ttttgttttt
    18421 ccgcttctag tcatgtttat tttaaccgga tggcgagctg atctagggat tttgcttact
    18481 atcattatct caatcctttt gttttctcta tttttgatta ttcttttttg tctggctagt
    18541 ttgattgttt ttggtttggc tcgaacagca gtgtttaaaa aacatagaaa aacagtttct
    18601 agtatcttgc ttggcagctc gatgctgatc gcatttgctg gaatcatgtg gatgaatcat
    18661 caaacgggga ctattgaaaa tgagttaaca gatcggcatc cgatttcgat attattgcca
    18721 ttctatgatg ctgccagtca gccactttct caacaaggtt tattgggttg gggaatacta
    18781 gctatcacgg ctctgggatt tttccttgct gtaaaacagc tgatagtacc aaagcttaca
    18841 gagcaactga ctgattcaaa cagtgagttc aaaataaaaa gaacccataa aaagaaccaa
    18901 aatgtgcatc agcttttatt tagttacaat gctcaattag taaaagaacc taatttgatt
    18961 atgcaagttc tttcttcttc cttgttaact ccaatcattt ttattcttgc ttttgcccta
    19021 ggaggcgaaa taaaattaac tgatttagat aataagttaa tcggtgttgt tttcctggta
    19081 gggatcgcat tggctttctt aacagttaat cagacttctt ttatcagcaa tcttatttct
    19141 ttggatcaag aaaattttct gtttatccgt tctttgccaa tctctatgaa ccaatattta
    19201 aaagaaaagt tcagattcgg atggatattg cagagttttt taactggtgg gatcgcatta
    19261 ttaagcggac tgtcttttca gctgcctttg ttttttatcc ctagcttgct atttggttct
    19321 ttattcggct gctacttatt gtcactacgt tatttcgcta gagattatcg gttgctgctg
    19381 ctgaactgga ccaatattaa ccaattattt aacagaggtt caggaagttt gggtttagtc
    19441 gcaactatga tgggttcatt gatcatcagt attattctta tcgtcttata cggaatgttg
    19501 gctttgtttt atccattttg gttagtgaat attcctgtga tcggtttagt tctaatactt
    19561 tccgctctat ggatcgttta ttatcaacga acgttttgga aaaaatttga ataaagaaga
    19621 ggggccgtga caaaaatcat gtcgcagccc cttttttaca gatagagggt gttcaaaagt
    19681 cagtttctca gtattaagtc aattttatgt aaaaatgtga gaagctattt ttacaaaact
    19741 ttttttaata ctcataatta cttcttattg ctcctgcggt tacttgtcgc aatgtttgtg
    19801 agatattttg tcatcgctca aggctgaacg tctctttcac aaccttttct ttgttacact
    19861 tcgtttattt aaaaagccgt ttttttcgtt tcatcatgat tgcgtcacgc tttactttga
    19921 aattacggat gttatggtta aagacttctg ataatacaag acctaaagcg attgctccag
    19981 caatcataat tgcttggacc gcataatgag ccgcttctcg ataggattca gtaacgagat
    20041 ttcgaaccgc ttgataagct aaaccaccag ggactagagg aaccatcccg ggaatattga
    20101 aaatcgttac gggcattttc aaacgtttag agaaaagatc gctgatgaat gctactccta
    20161 aagaaccaag caatgaacca aaagcagcag atccgcctat ttgaacacag atccagtaga
    20221 tcatccagcc aaaagcacca gaaagaccac aacaaatcaa tgaacgccta ggtacatttg
    20281 taataacggc aaatgccgca gtcgctaaaa aactaaaaga aaactgaata aatatcgtcc
    20341 ccatacttgt gttcctttcc taataaaaaa gctggaaaat aaaagcaatc gcaaaaccga
    20401 tcatcgtagc agtcatcatc gcttctgttc cgcgagaaac tcctgagaca taatgtccag
    20461 ctagcaaatc acggatagcg ttcgtaattt gaactccagg aactaaaggc atcacacatc
    20521 cgataatgat tagatcctga ttgcttccta agccaaaacg tacgctgtag atagcggcta
    20581 tgccaatcaa taaagaagat aaaaattccg ataaaaattt gattctaaaa aatttcaaac
    20641 tatatagata agctgcgtat cccaatcctc caatgactaa agtcacaggt aaatcggtca
    20701 tctttccgcc aaataacacc ataattgttc cactgatcac tgccgcgcta acgatttgca
    20761 accagacagg aaagaaagaa cggtctcggt cgattgcttg cagttcgcag taaagttctt
    20821 ctaatgttag ctctgaagca acatatttac gtgaaagatc atttactttt actacttttt
    20881 ctaaattaat cgaacggttg gtaatttgtt ccattcggat ggtcgaggta cgatcaagac
    20941 caataaagag accggtttga gtgacatagc tgaccaaacg gaaatttcca gaagctaaag
    21001 caattcgact catcgtatct tctacccgat acatctccgc attgctttcc atcatgattt
    21061 tcccagcgag taaacaagtg tccagcaaca gatctaattc ttttcccgtc atagcatctt
    21121 ctccaataat tgattgatta gttttatttt actacaaaac ataccagaag cgtaaagaat
    21181 aaaaaaactt gaggaaggaa taaaagaggc gtatactgtt tttgtattgc gggtgtagtt
    21241 tagtggtaaa atcccagctt cccaagctga tgtcgcgggt tcgattcccg tcacccgctt
    21301 aaaataggtt gtgaaaaagc tgctctgcaa caagtaataa gaacagccaa gcaaaaacaa
    21361 cttcttatgt ttttaacttg gatggactta ttaccgcaga tgcaagcttt tgcgcaccgt
    21421 ttaccaagcg gttgtgaaat cagtgtattg tcttgagtat gaagaaaatt tttgaataat
    21481 aataaatacc ttacagatag agtggtgact gattagagac gaggaatccg tctctttttt
    21541 gtatttttaa gccatcttaa aacaggtttt attttttgct gtttctattt ctttctagta
    21601 tttatttata ggggaacata tgttaagatt tagggagctg ttagtaaagg agtaaagaaa
    21661 tgagcaaaaa aacaaccaag gcgatcgtgg ggatcgtcgc tgctgtgatt gcaggggcat
    21721 tcggtgtttc caccacacaa aaagattcag atatctttca gcagattaca actatttttg
    21781 attccacacc acaagcttct caagtagcgg atgtgccaga atatgatgga aaacatcaag
    21841 aaatagaaat caatgggaat cagccggaat ttacaaaaga ggatcttagc ctttccaaag
    21901 gaacatggga aagctattca aatattgata aattgaacag agtaggacaa gccaatgcta
    21961 tgcttggaaa agatttgttc cctactgaaa agagagagcc tttgtatatt gacccgacag
    22021 gttggaaaca aaagaaatta agtgacgggc aatggttata taatcgcagc catttaattg
    22081 gtttccagct gactggtcaa aataacaaca ttaaaaactt gatgacaggg acacgttcgt
    22141 tgaatgctcc atacatgctt gctcacgaaa atgatattgc ggcatatatc aaagaaacaa
    22201 atcatcatgt acgctaccgt gtcactcctc attttgaggg gaatgaactg gtagccaggg
    22261 gagtgcaatt ggaagcagaa agcattgaag acaaaaaaat tgaattcaat gtctttatct
    22321 acaatgttca agatggctac acgatcaatt atgagacagg acaagcaaca aaaacaaaaa
    22381 attagtagca gctattcgaa ttttttatga atattatgaa aaaattgtgt aaaaacatac
    22441 attcaaagaa atatagcgta tactttatga aaagagtact tttgataaag ctacagaaaa
    22501 aaggaaaaaa acatacaaat agtgtttctt aatttattcg gaagtttgga atatttgttt
    22561 gtagttttcc ctttcttcga gtaaaataag gttgtagaag gtaagctgga attaccttgc
    22621 gggaaagagg tgtagaatca tgggttttac agatgaaacc gtacgttttg gttttgacga
    22681 tagtcgcaaa aaagaagtga gcgaaacatt agcgattgtt tatcgggctt tggaagaaaa
    22741 aggctacaat ccaatcaatc agattgtcgg ctacttgctt tctggagacc cggcctacat
    22801 tcctcgttat cgtgatgctc gaaatttgat tcgccgtcac gaacgcgatg agatcatgga
    22861 agtaattgtc aaggactacc tctcaaatca tggagtgaat ttatgagagt aatgggcttg
    22921 gatgtcggtt ctaagacagt aggtgttgct atcagtgacc cctttggctg gacagctcaa
    22981 ggagtagaga tcattcggat caatgaagca gaagaagagt ttggctttga tcggttgaag
    23041 gaattagtag cacaatatga agtagaccgg tttgtagttg gattacctaa aaatatgaac
    23101 aattccatcg gaccaagggc agaagcttcc atgtcatacg gagaaaagat caaagaacta
    23161 ttctccttgc cagttgatta tcaagatgaa cgattaacca ctgttcaggc agaacgtatg
    23221 ctagtagagc aggcaaatac ttcaagagcc aaacgaaaaa aagtcatcga taaattagca
    23281 gctgtgatga tcttacaaaa ttatttagac ggctcaggta agcaattatt ttaataataa
    23341 aagaaaagag gtaatcttat gacagaacat aaccattccc acgaccatga aggacacgaa
    23401 cacatcacat tggtggatga ccaaggaaat gaaacattat atgaaatcct actaacagtt
    23461 gacggacaag aagaatttgg acgtaactat gttcttttat atcctgctgg tgtttcagaa
    23521 gatgaagatg tagaattaca agcttatgca tacatcgaaa acgaagatgg aacagaaggc
    23581 gagttagaac aaatcgaaac agacgcagaa tgggacatga ttgaagaagt attcaataca
    23641 ttcatggcgg aagaagaaga atagaacaaa agaatgaatt aataagatat aaaaaaaggg
    23701 attgtgacaa acgtcttgtc acaatccctt tttccaaata aacgatgttc aatcagctgc
    23761 ccctcggcat tcgctcgaaa aacggcgaaa tatgaagaac tattttgcca tttgtttctc
    23821 taataatcag gtcaaaacgt tgacttcaca gtctcttcct taaacggtgt tctcctgcca
    23881 actcctcggt cacaagtcac aatattcaac aaacatgaga agctgttttt tgaaattgtt
    23941 ccttgtgact cggagctgaa cggcaggctc acgacctctt tcttaaacgg tgttcaaaag
    24001 cttgcatctg cggtaataag ttcaaccaaa cgaaaattat gaggagctat ttttgtttgg
    24061 ctgttcttat tacttgatgc aggacagctt tttcacaacc tcttttcgtt tagcactaaa
    24121 tttccttaaa aagtagaaaa gctttcgaag cctcgtttgt ttcttttact gtataataaa
    24181 agaaaagagt aacaatcgct aaaagatata aacaagagaa aggaaataca agatgattgg
    24241 tctgatttat tttgtggtga tcgttctggc gaacacagtt ggcgctattt ccgggatggg
    24301 tggaggcgtg ttgatcaaac cgattcttga tcttatcggc gcccattctg ttgcagggat
    24361 ttctttttac tcaacagttg cggtattcac gatgtccatt gtttcgactg tgcgacaagt
    24421 atcaagcggg aaatcactga attggcagat tgtcggttgg gtatctagtg gagctgttgt
    24481 tggaggagta gtcggcaaca ttgtattcga agttttttta cagttatttg aaaacgagaa
    24541 gcatgtgcag ctgatccaaa tttttttgac ggtattgacc ttggtttttg cgttcttcta
    24601 caccaagcat catcagccaa aatttcattt gacgtcatgg acttggtacc ttatatgtgg
    24661 ggggggttct gggcttttta gctagttttt taggtattgg tggtggacca gtcaatgttt
    24721 ctttgttgat gctgatgttt gcattgccaa taaaagaagc aacactatat tctctatcta
    24781 cgattttctt ttctcaactg gcaaaattgg taacgattgc tttaacaagt agctttatgc
    24841 gctttgattt atctatgttg ttttatgtta tccccgctgc tgttattgga ggtttgtggg
    24901 gagcaagatt tagcagggtt ttgtctccaa aaaaagtgac ctttattttt caagtaattg
    24961 tcctcgtggt tcttttggtc aattgttaca atgcctttgt catcttacaa acattaggag
    25021 aatctagact aacgcaagtc aaaatcactg aaacatattg ttctaaatga aaaaaagagt
    25081 atactatgat aaaaaaattc attttaaaaa atcagaaatt ggctggtgat ttggatgtta
    25141 cgggaaatgc gtttagaaga tgcagaagca ctacgtgaaa tcaatgcgga tcaattaggg
    25201 tatgatatac cattaaccgt tacggtgaga caaatgaaga aactgctgaa ggagccagag
    25261 aaaaattttt tcttggttta tgaagatcct acttctaaaa aagcagctgg ctatgtacaa
    25321 gcggagtcct atgaatcgat ttttagcgat ccaatgttca atattatggc acttgctgtg
    25381 tctaaatatg ctgaaaaaag aggcatcgga aaggctttga tgactggcgt agaagaagaa
    25441 gcgaaaaaac gaggaataac gacaataagg cttaattctg cagaatacag agtagaagca
    25501 caccaatttt atgaacacat cggttatcat tccgataaaa tgcaaaaaag atttttgaaa
    25561 atcctgtaaa tctttatcaa aaaaataccc gatcaccaag ccgatacttg atgatcaggt
    25621 atttttgttt ttttgactaa gaaagtaatt gcatcaagaa accagagatg atcaaaacga
    25681 tggcaccacc aagtcgattg cccatttgag caaaagcaat aagttccatc cgattagctg
    25741 cagataagac ggctacattt cctgttccgc ccatcgagtt gttgcataag ccagcagtaa
    25801 tagttgattc aattggatat aaaccaaaca aacgaccgac aaatcctgaa acaactgaaa
    25861 tcgcgataac acttgtcaga cataagacaa caaattgcca tgtcaaagca gaagctaata
    25921 cattcagatt cagcaacgca ataccgatac cggcaagaac agctgctgtc aagtttttga
    25981 caatcaagtt attgaacatg atagctgcat cttcataata ctctggtaat aggttggcga
    26041 ttttacagat gacaacgata ataatcataa atgcgtaagc atggacttta ggaacaaaat
    26101 agttaccaat ttgtcctaag atgaagaaag tcaatgatac catcaaacca acacctaact
    26161 gtggaatgga aggtttcaca gttcgaggtt ttccatcgcc aagatcgcct ttttccatta
    26221 atttaccatt gccgttgaat tttggcattg attgtccagc acgagcaact aatgctgcac
    26281 cgatgatagc taagacattt gtcattgctg tcgcaggaat caggcgggaa atgatagaag
    26341 aagcatccgt acctaaaaca tttgcataaa tcgttgataa aggaacggct ccggctccaa
    26401 caccaccagc catcgcaggt aatgagatgt ataaaacgga atttgcaaaa ccatttccga
    26461 taagcatgcc cacgagtcct acggcaaaaa aggagacgac cattgaaata agtgcaacgg
    26521 gaataaaacg aacagaagct ttcatcaata gattacggtt cataccaagg atgctaccag
    26581 taatcaaggc agcaatataa aagtcccaaa agcccatatt gctgacaaag ttttctgtgg
    26641 atttcactac atattcaggt aagatcccaa atgtagcaat cgcagcagaa gcgaagatcg
    26701 caaaaacgga accaccaccc aaataacttt tgacgatagg gatttttgtc ccgataaaat
    26761 gaaataaatt accgaaaata accaaaactg cgattggtcc aatcatattc gtcggtaatt
    26821 tccctaaagc cattgcaatg atcaggacaa ggaccatgat caaataaagt ggcaaactta
    26881 cgccggtgat ttttgtggac caaaaacctt cttttttctt ttcctcaact gcagttgcag
    26941 ttgttgtttt ttgtgccatt tttacagatc ctttcttatt ctgtatcata tgaaaaaagc
    27001 caaagaaaag cgacaaaaga gcaggagttg gctggccgct gttttgccgc agtcgctttt
    27061 ttctttttag acgtcataca aagtttctgt atgttctttt actataacga tcttaatctt
    27121 ctaaagattt atattcagga acccatttag cagattcaac tgcttctttg atatccgtga
    27181 tttcttcacg attcaagttt tgtgcgacaa ctgattttgc gacagtttca gcaatcgttt
    27241 gagaaaattc agtgattttt gctactggag gcaagatagc tgctcctggt tttgttacat
    27301 caacgattcc gcctaatgct cgactagctt gagaaatgat ttcagcattt actcgtgtag
    27361 ctgttgaagc aatcaaacca agtcctaaac ctgggtacat caaagcatta tttgcttgac
    27421 cgatttggta agttacacca ttatattcaa catcagcagc agggatacct gttccaacta
    27481 atgcttttcc gtccgtccat tctattaaat cttttgcttt tgcttctgcc aattttgttg
    27541 gattagacaa aggcatgatg atcggacgtg gtgtatgagc agccatttct ttcacgattt
    27601 cttcagtgaa tgctccaggt tgggtagaag taccaatcat gattgtcgga tggatcgctt
    27661 ttacaactgc ttctaagttt gtcaattcat cagcattagt gaaatctgag cgtttgcgag
    27721 cgaatgggat ttgaccagga gtcaatcctt ctgtatcttc aaacaataaa ccttgtttat
    27781 ctacttgata gaaatgttgg cgtgcttctt cttcaggtac accttgacgg atcatctcat
    27841 ctaaaaggat gtttgcgata cccacaccag cagtaccagc accaaatgtc aaaattattt
    27901 gatctttcaa ttgttcaccg gatatattca atccgcctaa tacacctgct aggacaacaa
    27961 ttcctgtacc ttggatatcg tcgttgaaag tagtgatttg atcttcgtat ttttctaaga
    28021 tcgctgcagc atttcctcgt ccaaaatctt cccaatgcaa taacaattct gggaataatt
    28081 cggtagctga ttgaacgaat tgatcgatga attcatagta agtttcgcct tctacacgag
    28141 catgacgatt gcctaagtat aagggattat tcaataattc ttcattatta gtaccagcgt
    28201 cgatcgatac aggcaaaact tgtgctggat taataccagc agcagcagta tagaccatca
    28261 atttgccgat agcgatgtca acaccgttca caccccagtc gcccatacct aagattcctt
    28321 ctgcatcagt cacaacaatc aaacgaatgt ctcgtccatc tgccgcgttc ttcaaacttg
    28381 ctttgatgtt ttctggttcg tcgattgaaa gaaatgcagc atcttgcggt ttcaagaaaa
    28441 tttcgttgta ttgttcgatt gaatccgcaa cgacaggatc ataaacgaca ggcatgaatt
    28501 caactaaatg ttcgcccatc aatttataaa acaatgtccg attcgtattg aatagattca
    28561 ttaagaagat acgtttttcc aaatctgttt gtttgcttaa gtattgacca taagcttgaa
    28621 cagattgttg ttccagtgtt tggacagtac ttggaagcat accagtgatt ccatattttg
    28681 cccgttcttc ttttgtaaaa gctgttcctt tatttagaaa aggattattt aataagttta
    28741 accctgtaag catgaatgat tcctcctaag ttttaataca aacgaagtat agaagtcaga
    28801 tgatgatata gtcaaaaaga gcaggtatga taaattttat ttatatgtaa tatagccaaa
    28861 agaaatcaaa atagcagtat ttgttctttt tatatcgaca atccttgctt gaatactatg
    28921 cgtatctctt atatgctaga gaaagaaaag tagaagaata agggatagat ggaatggata
    28981 aataggattt ctgaggaaaa aagcacatga tagcgttgat aggagtcacc tatgtcaggt
    29041 gataaaaata cataagtaga aaattaagag gaggaagaga aaatgaaaga agttcaattg
    29101 attagaaaaa gcgaactatc agaaggcggt tgcaatgcat gtggcgtagt agaagcgaca
    29161 agctatacat taaaattaga ttccaatcaa gcaataatct ctgaactgac agtaggcggt
    29221 cttgtggaca gtctggcgct tgctgaaggt tttactggag aagatattta tgagatgttt
    29281 tcagaagtcc gtcagttaaa aaaaggagaa aaatgcatag aggttcatca tgagagcccg
    29341 aatgtgaggt tcaaacgtgg tgataatgag atgatcttca agaatcatgt gtctgatcac
    29401 actgaactat atgaaatcgt gaatcaaata ctgtctggga tttttgaatt aggaccttat
    29461 gagttcaagg aagaaaacgg taatccaaaa ttaaatgaag aatggcaaga aacgattgaa
    29521 acacaaagaa acaatccaca cttatttcaa tagtcttcca cagtatttcg taaaaatgtt
    29581 gatgaaaaag aaacaccgtg aatcagaggt cgtgaaaaac ctgccctgca tcaagtaata
    29641 agaacagcca aacaaaaata gctcctccta tttttcgttt ggttgggctt attaccgcag
    29701 atgcaagttt ttgaacaccg tttaaggaag agactgtgaa gtcaacgttc tgacctgagt
    29761 attagagaga caaatggcaa aatagttcct catattttgc cattttttga gctaataccg
    29821 agggtcagct gattgaacac cgttttatcg agaggttgtg aaaaagtcgc ttagtttcga
    29881 gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt tgacttaata
    29941 ccgagaaaat ggcttttgaa caccgtttat tcaaaaaaag ggactttgac actacttttg
    30001 tcaaagtccc atcggttttt tatctatttt tattacaatc atttcttcca gagtagtgac
    30061 atctggaatg gctgaaatgt aaacagatta tttgcctgta tcaatgtcga agagtaatat
    30121 aaaaagtgta aacgctatca tattgttcat cattcaaatt ttatagagag aaggcattga
    30181 gatggaaaaa acatatcaag caggtacaaa tgaaggaata gtagatttta tcaatatgga
    30241 agatttagaa atcgcggctt cacaagtgat tccagctggt ggttatggtt acatcagcag
    30301 tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc atcgtttgat
    30361 tattcctcat gtattaagag atgtagaatt acccgataca actacacatt ttgacgaaga
    30421 aatgctgact gcaccaatca tcatggcacc agttgctgct catggattag ctcatgtgaa
    30481 ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata cagcaagttc
    30541 ttacgcttct tgtactttag aagagataag agaagcaggc ggggagaaag caccgcaatg
    30601 gtttcaattt tatatgagca aagataatgg aatcaatttg gatattcttg aagtggccaa
    30661 gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg gaaaccgtga
    30721 aacagaccgc agaaacgggt ttaccttccc gctgccaatg ccgatcgttc aggcttatca
    30781 atcaggagta ggccaaacga tggatgctgt atataaatct tccaaacaaa aacttagtcc
    30841 aaaagatgta gaatttatcg ctgctcattc tgatttgcca gtctacgtga aaggtgttca
    30901 atcagaagaa gatgtctatc gatcattaga atctggtgct ggtggaattt gggtatctaa
    30961 ccatggtggc cgtcaattag atggggggcc tgctgcgttt gattcgttgc aatatgtggc
    31021 agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac gtggacaaca
    31081 tgttttcaaa gcaatcgcat caggtgccga tcttgttgct attggcagac ccgttattta
    31141 tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct ttaaaacaga
    31201 attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca aaaaaataaa
    31261 attgcgtgag aatcgtttta tttaagaaat aaatgacgaa aaaaatagga gtgcggcaga
    31321 tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa tttgaaacga
    31381 aaaaataaaa ttgaaatatt ttttcatact taataaaaaa ggaagaatat tcatgtaaac
    31441 gattttattg tttttttctc ttttatacaa aaagggtcat aatttcaagt aagcggatga
    31501 attcatgagg attttttgca tactgtctct actatactta ggtcactaag aaaaaggagt
    31561 gatttattat gaaaaagaac atgattggga cattattact ttcaacactt cttctaggcg
    31621 gcttggcaac accagctttt gctgagggtc aagcgacttc aaaaggagat attaccttta
    31681 ctgaaccaac aaataccgtt gagcctctta accctactga tccttcaaaa ccagtagagc
    31741 cagcagatcc ggaaaatcca gcaactggcc agacaggttc tttaacattg gatgtagttc
    31801 ctgaattgcc atttggtaca cacgagattg aaagcggaac aaaaacgtat caagtagacg
    31861 cttcaaaaaa cgatacacct tatctccaag tatcagatcg tcgaggtgta ggtgcagatg
    31921 gccaagcaca agggtggaac gtgacagttt cggtttcaga ttttgtaaac ggttcacaag
    31981 ttttgcaagg cgctgaatta gattttggaa catccactgt taaaagtacg tcagataacg
    32041 aatccactgg accaactagt caaactgtaa ctgggttgag taaagcaagt gcagcgacac
    32101 caatatttac agcagcaaaa gatcaaggtc tgggaacatg gctttctgtt tatgatccag
    32161 caaatatcac actgaaagtg ccgaaagcag cagctggaac attcacggct gatttgactt
    32221 ggaacctcgt ggctggccca gtagcataga gtatgaaata aattcaacaa actgcggtga
    32281 aaatactata ggtgaaggat tttttaccgc agttgttgtt gaaaggatgt ggtggacaat
    32341 gaatcaaagt aaacgactgt ttgtttcgtt tttttctatt ttaagtctat ttttcatttt
    32401 tccgtcaatc agccaagcaa aggattcagc aggagacttt ggaatcaaac cggtatttcc
    32461 agagaaccaa atagataaag caatcggtta ttttgattta ttagtggcac cggaacaaaa
    32521 tcagacacta gaagtaatta tcagcaattc ttcagatgaa gaacgaacat ttgaggtctc
    32581 tgtcaatccg gcggtaacga gtgatggtgg aacgatcgat tatagtcaaa aaaacccaac
    32641 gttagatgag acattgccgt ttgatgtccg tgacgttttg ctcattgcga aaaaggaaat
    32701 caatgtatca gcacatgcag aaacaactat acctatagaa gtgaaaatcc cagcaaaaag
    32761 ctttaaggga cgtgtattag cgggtattca tgtttcacct aaagaagaag ctgaaacaga
    32821 aaatgctaca gaaggagcac aaatcaaaaa tcgtattgct tataatctcg cagtcgtttt
    32881 acaagaaagt caagagacga tagaaccgga tctgaaactt ttatcaggag atttggacga
    32941 ggtcaacgca aagccaactg ttcagcttcg tttccaaaat cctcagccta ggattatcag
    33001 caatttgata tttacgagca agatctttta tgaaaatcag ctttatattg aaaatacgtc
    33061 taatgaattt ttagttgcac caaattcgaa ttttcacttg aatcttgatt tagctggtga
    33121 taaagcaaaa gccggcgatt accgcgcaga aatcatagca aaatcaggcg acagtaatga
    33181 atggcgattt acacaaaact ttacgatcaa aaaagaaaaa gcacaaaaag taaatgaaaa
    33241 ttccgttttt gcagtacaag aacaatcttt cccatggctt tatgcaggat tggttgctgc
    33301 agttctagtg ttatggttga ttttgctgtt tttgtgggtc cacaagaaaa agaaaaaagg
    33361 agaaacaaca tgatgaggtg atttcgttga aaacatcgat cttattggga gggattattg
    33421 caagtttgct gttcttcagc tggcaagagc cagtccaagc agataccaaa agttcaggta
    33481 cgattacatt ttatgatcta agtccagttc cgcatccaag tggttcagtc aatggcgcat
    33541 caaaacagca gacacagcta ccgatttata aagaatctaa tcaacatggc gtattcccgg
    33601 agacgaatga agaaaagatc cgttttcttt atttgatcat tggtatagaa ttatggcttc
    33661 ttgcgtggtt gttgttatgg agaagaaagg aaaaggagga tgaacatgtt gctacaaaat
    33721 aaattttcat tttttcgctc cttctttttt acttcgcttt ttgttttaag tatatttatc
    33781 ttttcacctt cggcagaagc tgttggagca acgcctcctg ccatttctta tgatccatac
    33841 acgtatgaaa atatcgtacc tccaaatggt ggacgtagtg cagtcaatgg cgggaagctt
    33901 gtccaactaa ctggggctaa ccaattgcct aaattgactg ctacttattt gaacgatggt
    33961 tcaaatgatc cttggaaagg tggcacggtc cgttttcatt gggaaagcca tgtgaaacta
    34021 acaaatgtac ttgatttggt gaatatcact gcacaaggat ttgcatttgt ggcgatcatc
    34081 aatttgccgg aacatgtatc aagcagtgat gtattaaaag caattgactg gaacaacgcc
    34141 tacttgtata ttgccggtgc ccccatcaag gttactagcg gtagtttatt atcagcagga
    34201 agccatacct tgcgttttgg gatggggtcg tgggatacaa atactctatt ggcagatatt
    34261 attgctatta ttacgcgagg cgaattcaac gtggacgata tcccgcttca atttgatgtt
    34321 acagctaatg tcgcgaagat gacagaaaac ggagataaat atgatacgag tccaaacaaa
    34381 attttgacca agggtaaatt acaaccttct atgtctaaaa aggccgtttt ttctgttgat
    34441 ttttatgatt cgaataatat tatccaagat cgtatgttca attcgatttt agctccagct
    34501 gcaggtagat tttatcctaa attaactggc ggaaacttga atccaggagc atatgcattt
    34561 tctgcaagtg cagagataaa tacgtggaat agttacatct caacaagtga ttcaacagga
    34621 aaatataata catttgctga taccacagaa tacgtggatg gaaataacca gaaccttcca
    34681 ggctctacca atagtcggac attgacgatg gggctgactg aaggacgttt tgatcagttg
    34741 ccatttagtc gctttgatcg cgtagtcaac tattttacag gaaaaaacgt tacacaagga
    34801 gcaatactga ctcatactcc agcaagcaca agtggattga atcagacagc caatatcgtg
    34861 tacagtggga aagatgcgca aggcggattg ctttctcctg ttgctttaaa ggtattggag
    34921 cagtacaagc tagacggaac ggttttacca acaaacttaa cagccgatag tcagtgggga
    34981 aagctccttt cttctgcatc gtaccaagtc acggccaatt ggtcagcaac tgaactaaaa
    35041 aagggttcga tccattacag gctttattcg aaaggaaccc aaaaattagc gacaggatgg
    35101 acagatcaac ttttccaaac gatccaatca aataacgggg gtgtaaatac agctacttct
    35161 acactacctg cattggcttc agggcaatat tattttgatt atcgcttggt ggacgatgta
    35221 cttactaaca tgtatccgtc ttttgcgtac aaatggcagt ctgaacaagc tggaatcacc
    35281 actcttcctc aaataacagt ggctaatttt ccatctgtga gtgcagatag tcagctaaaa
    35341 aaccttagca gacaacctga gacaggagat cccctgtctg ctctatcagg agatacgatt
    35401 caagaaaatg tgacattcac gttaacaaaa tctggggatg cgataaccga caaaaaaatc
    35461 acgatctcac ttcctgaaaa tactacttat atcaatggtt ccttaaaatt aaatggtaca
    35521 actatcgcag ataccggcat tcaacaagga gtttctgtcc cagcagatct attgagcaaa
    35581 ataggagata ccattcatct tacttacaac tatcaattaa atactgtaga tacttctgta
    35641 caatccgtca gcatactgac aaaggcagct gtactttcca gcaatattac attagcagat
    35701 ggtgctaagc ttcctaaccc agttgtccaa acatctgcta aaacaattct tgtccccaaa
    35761 caagaattaa cattagttaa tgtaccagat gattttacat ttggtaacga tctccccaaa
    35821 ccgttgaaga caagttatta tgaagcaaaa ggcgattttt cttttgatgt gcgggatact
    35881 cggcttccaa gcacgtctcc ttggcaactg acagggacat tgacttcttt atttaagaat
    35941 gatcaaggtc aagaattaag cggaacaaaa ttatacttca atcattctgg atcaaaacaa
    36001 ttgattcagc aaggtcaaaa cacgttgatt tatgagagtg atggaacagc taaaggagag
    36061 gttctcgttg actttcccga taccgatggt ttactcttag aggtaaatag cagtacgaat
    36121 gctcaacctg gcgcaacgta tcaaggcatg gtcacatggg aactgacagc tgggccaacc
    36181 agctaagtga ttagaatttg tatggatctg tgataactgt cacagatcct ctttttcaag
    36241 gatgagaagg aaacttctgc tgttaaaatc agaaatgctt ggaggagtgg acttggaact
    36301 tttcagtttg cttgaaaaaa atgaacaacg gcaaatcaaa cttttccaac ttttattaga
    36361 ggaaaaagga gagatttccg ttaataacgt acttacccgc ttatcagttg atcgggctac
    36421 cttgaaagaa gatctgtttt atttacaagc taacttggaa aatatcaaac aattggaaat
    36481 tgttatccaa gaagggaata ttttattaat cagacatgga aatatagcag cagtcgatgt
    36541 ttattatcat tgctacttgc gctattctac aaaatatcag atacttcttt atctgttgga
    36601 aaatggctca tttgatcggc agaaacttgt catggaactt aatatcagtt cggctacttt
    36661 gtctcgcaga gtgaaggaat taaatgattc tttaaatgag tttcatctga aaataaaaag
    36721 cgggcggctg accggtccag aaagtcaaat ttgttatttt tatttccaat tattttggtt
    36781 tggacgtcct tatcttgaaa acaaaaaaga atacagtgat ttatcaggaa acactttttt
    36841 agatctgctg caaaaagagt ttagttttac tattacagaa gaaggacgat tgaaatttga
    36901 aatatggctt tccattatga agaagcgaat gcgctgtacg aaaaaatcca aaccagatta
    36961 tagcaatctt cctgttggat tgtttcaaac gacacaattt ttattggacc tccatacatt
    37021 tttgacccgt tacttctttc aatatgcctt tccttggaat caaaatgaaa cgttggtttt
    37081 ttatcttttc atgatcagta actttacatt agatacacat aactcgcatg tccaggagtt
    37141 tttgataagc gaaaaataca aagacgacgt agtagctcaa ttgaatcgaa cgtttaaaca
    37201 atcgatagaa gaatatttct ctttttatgt ttttccagaa gagatttcgc aaaagattgc
    37261 gttgacagtg atgcaaaatc acgctcattt tgcctatttt agaggatgga tgaatatttt
    37321 tggccaagaa ggattaaaac agcggttaac tgaagtagaa gataaacaat tcctggtttt
    37381 atgtgaaaga ttggtcagac aaacatttgc tattgttgat tttactgaag tagacgagga
    37441 aaatgcccgc tttgaattat ttgctcgcta tgcatctatt ttgcggacgc tgatcaagca
    37501 gttagaatta cgtctgcaaa ttgcttatga tcttccttat gaaaggctaa tggcggatat
    37561 cgtgaaagaa atcatccagg aacaattgct gaatacgatc aaatatgatt tagtcgaata
    37621 cgagaaagaa aagcagtatg atatcatttt gactagccaa ttgaaagagt acccgtcaca
    37681 gaagtccgct aaagtttttg tattcactag caacgaatat aattatgatt tcccctattt
    37741 gaaccaattt ttaaaagaat gttatttaga aaaattgaat ttaaggatga ataaaacatg
    37801 aaaatcgtga tcattggtgc ttcccatgca ggcatcacag ctgccctaaa tctaagaaaa
    37861 cttcaaccga aagcagaagt tttactgatc gatgaagatc ataaagatgg actaggctat
    37921 gtttcaaatg gaatcaacct ttatttaaaa ggaaagatac gtagtttagc tgaagttgcc
    37981 cataacatgc ggactcttca agaaagtggg gcaaaactga tcaccgaatg gagagtaact
    38041 gaacttgatc cagacaaaca tcagctcatc ctcgcatcca aagaaggaaa aaacgagacc
    38101 ataacctatg ataaattaat cgtagcaaca ggttcctctc ctgtcacctt gtataaacaa
    38161 attgaagctg aaaatgtcta cacctataaa aatctagtgc aatctaagca agtacttgcc
    38221 gctttaaaag aagcaaaaga agtagtgatc ttcggtgctg gttatattgg attggaatta
    38281 gcagatgctt tgagaaataa aggatacatt attcatctag ttgattatat gccgaatgtt
    38341 ttatcaagat atttcgataa ggatatgatc aacagttttc aaaatcagct gcaaactaag
    38401 caaatcaatt tttatccaaa tgaattcttg atcgattgga aaaaatcaga agagaaagta
    38461 gtatccgtac aacttctatc tcaagcaata aaggcagata tggtcatttt ttctgcccaa
    38521 actcgtccta atacgacatt gttaaaagaa aaagtggctt tgtatgagga tgagactgtg
    38581 atagtcaatg aatatctgca gacaagtgat ccggacatct atgcgattgg agatatcgtc
    38641 ccagtcagtt ttgacaaaaa taaacggcat ctctttttac ctttagttac tcgagctgta
    38701 catatggcaa gagcagtagc gctaactttg agtggtcagc caaccgctta cgacctcaga
    38761 caaaagataa cagcagcagt cattatagac tactttttag gaactgtagg gctgacagaa
    38821 gatgaagcgc cattcttgga acaaaatacc ggttcgtgtt caggagaatt tgatctattt
    38881 ccacaatacg atgaagaaaa caaaacagta aatgcaaagc tgatctacca tccggataca
    38941 ttagaaataa ttggcggaca gctgataagc caagaattct tattatcaga tttgaacctg
    39001 cttgcggata tcgtgaaaca caaaactacg attccacagc tagcagttga ggattttgga
    39061 tttctgtctg aacacacctc tcgttttcac tacctcaatg agttagcttt taaagtacta
    39121 gctaagagag caaatagcaa tagagtagat aaaagactgt gagaaatatc acggtctttt
    39181 attgtgtggg gatataataa atattaatca atcgagaata aaaaattcat ataatgaaat
    39241 agcggaagaa ttttttgagc ataatattcg ccaaaaattt atcaagtgat aaaattgttg
    39301 tataaaatga ttatttcagg aggaggagtt aaatgttttt agcattaaat gaaattatgc
    39361 attcaaagtt acgttatgcg ttagttgcag gggtgatgtt tttgatcgcc tacctggtat
    39421 tttttttgac tgggttagct tatggcttag ctcaagataa tcgaacagca gtcgacaagt
    39481 gggaggcaga ttcgattgtg ctttcgaaag atgcaaattc aaatctaggg atgtccatga
    39541 ttactaagaa gatagcagaa gaagtcgaag gtgggaaagt ggcgtatctt gcccagacac
    39601 ctggagtcgt gactagtaaa gacagtacag aagaaggaaa aatcaatgtt agcttcttcg
    39661 gtatcgataa aaatcaattc atcatgccca atcttgttga aggaaaagct tttgataacg
    39721 atgatgaggc agtaggcgat attagtttga aagaagaata tggtctagcc gtaggagata
    39781 cagttaagtt atctggtagt gacaagacgt ttaaactaac tggctttacg gatcatgcca
    39841 aattcaatgt ctcaccagtt ctctatacaa ccatcaatgc ttaccaagag atccgcttcg
    39901 aaaaagaaga tacaagtgaa aatgcacgga ttaacgcaat agttgtcaga ggtaaaatca
    39961 gtaatttacc tgaagattta gaacagatca aaatttctaa attcatcaat gaattacctg
    40021 gatataatgc gcaagtattg acttttggat ttatgatcgg atttttgatc gttattgcgg
    40081 ctattgtgat cgggattttc atttatgtac tgacgatgca aaagattaat atctttggtg
    40141 taatgaaagc acaagggatc acaggaggat ttattgcgcg ttctgtagtg gcacaaacat
    40201 tcattttatc atttgttggt attcttcttg gtctattagg aacagtggga acatcccttg
    40261 ttctacctga tgcggttcca ttccaaagca attggctgtt ttttggcgtc attagcttgt
    40321 tgatgctggt agtagctgtg ttaggcgcac tattttctgt tcggacaatc gtaaaaatcg
    40381 atccgttgaa ggcaattgga taagaaggag gaagagcatg aaagcaatag aatttaaaga
    40441 agtagaaaaa agtttccaag atggcgacca agtagttaaa gcattgaaag aaacaaccat
    40501 gagtatcgat aaaggggaat ttgttgcaat catcggtcct tctggttcag gtaaaagtac
    40561 ttttttgact ttggcaggcg gattgcagac gcctagcagt ggctctgtat ttatcaatga
    40621 tcaagaattc agtaatcaaa aagaaagtga acgcgttaaa ctacgtttta aagaaattgg
    40681 ctttattttg caagcttcca atctggttcc ttttctgaca gtaaaaaagc agttattatt
    40741 agtagataag atcaaacatg aacatcgtca aaaagaagca aaagaattat ttgagcagct
    40801 tggcgtggat aagttgatca acaagtatcc agaagaacta tctggtggag aacggcaaag
    40861 ggtcgcaatc gctcgtgcat tgtacaatga tccgtccatt attctagcag atgaaccaac
    40921 tgctagtttg gattcagaaa aggcacgaga agtagtcgat atcttggcaa aagaatcaaa
    40981 agacaaaaat aaagcaacga tcatggttac tcacgataca cggctgattg acaaatgcga
    41041 tagaatattt atgatggaag atggtgtatt gaaagaaaaa gaataaccaa aaagcaggtg
    41101 gaaaaatggt ccatcgatac aacaaagata cacagcaaaa aattttgaaa gaagcgactg
    41161 acttgtttat gtccaaggga tatctcggca cttcaacaag agagatcgca caaaaagttg
    41221 ggatcaccca acccaatctc tatcattatt tcggtgataa agaaaaacta tatacggcag
    41281 ttttagaaaa ccacttaaaa gatgttggaa aagctttgag agaaatcgtt caaacaagcg
    41341 aaacgggatt tcaagtaacc ttgacaaaaa tggcgcagtt tttgattgag acacatctgg
    41401 tcgatttgtt tctgatgctt cacgatcttg aaagcaatct ttcaaaggaa acaagagatc
    41461 atcttttctt tctatggaag aaaaattatc gtgaaccttt tgaagaaatc ttttcagcaa
    41521 accaatctgt gctaagaaat gggatttcaa aagaaatcgc agcaaggcat tttttcttag
    41581 tgttagcacc ttacatcact cggtcgaaaa gacctgtgga agaaccgcta aatgtggcgc
    41641 aattgatcga tttatatttg aatggtgtat taggagaagc gtaaagctgt tatataggga
    41701 gaaaaatagt taggtgaaga agcaagatat gacgaagatc gttatatctt gtttttttta
    41761 tataggtagt ttttcataaa tacaaatttg ttattctctc atgattatca gggtatattt
    41821 ttatttatta tgtcggtgtt ccaggaagtg ttttaattta ataacgaaga aattaattgt
    41881 ttgtagaatg aaggaaacta tttattgggt agatgaatca ccacgaatag tcaaaaggag
    41941 atttcaggat atgccaaaaa tgaaaaaaat cattttagca gtaatgttat tttctacggt
    42001 tatatctttt acagcccctt ttttgcgtac ttaagctgat tttaagtttt gacccttagt
    42061 ctggatctac gaggtgagag tcaagaatga aaaaaataat tggagctttt gtatttgcaa
    42121 tcattatcat tattatctgt tttcaaatag atgtttttgg tcaaaaaaag atagttggac
    42181 aaacggtcag cagtacgaac agtacggtta caaagacaaa ggaatcattt gatgatacag
    42241 aacaagagac attaatcaaa atcggaaaaa atgaccgtac actatataca gatgactcgc
    42301 taactacccc acttgcaact ataaacggcg gagagctggc ggagtttctt tctgaaacaa
    42361 aaaatgctta tcagatcaag acaaatgatg gctatactgg atatttagcc ctcgaagatg
    42421 gaaccaaagt cacaaagaat atacaaacaa aaccggaaga gctatctgat gcagtgattg
    42481 tattagatcc tgggcatggc ggaaatgata ctggggcatt aagcaatgat gaacaaacag
    42541 aagaaaaaga tataacatta agtacagcca taaaagtaaa aaaggcgcta gaggatgctg
    42601 gagcaacggt ttatttgacc catactacag atgaattagt acagcttgga gatatatgcg
    42661 actacagtga agaaaaaaag gcagatgtct ttatcagttt acacgcagac tctacggaat
    42721 atgcgaatga agcaactgga atcacgacat attattacta tggacaagag gaaacattag
    42781 cacagacaat tgctgactcc ttcactgatc tgcctctgga ttcaagagga atctccactg
    42841 ggaactatca agttcttcga gaaaatcttc aaccttctat tttaatcgag atgggttata
    42901 tgaataatga tagtgattta aaggaattag taacagacag ttatcagcag cagattgctg
    42961 caagtttgac acaagcattg accacttatt ttcagtaata aataagccca gccaaacaaa
    43021 aatagctcct catatttttc gtttggccgg gcttattatc gcagatgcaa gcttttgaac
    43081 accgtttaag gaagaggttg tgagcctgcc gtttaactcc gcgtcacaag gagcaatttc
    43141 aaaaaacagc ttctcatgtt tgatgactat tgtgacttgt gaccgaggag ttggcatgag
    43201 aacaccgttt attcggaaca agggactgtg acatgacttt tgtcacagtt ccattatttt
    43261 tttagcagct tcaaagcttt ttctgtattg gcaaaatgaa gtttggcata tttttcaagc
    43321 ataggcatgc cgcctttctc caatagtttt gccgctactt ggtcagattt cgttccagct
    43381 cctgatggaa gggtgattcc agcttcagct gatgctttgt gtaactcttc caaaaagctg
    43441 gcacgactga tgatagaagc agcagcaaca gctaaatgat attgttctcc tttagtaacg
    43501 aagtacagtt tttcttgtaa ttgatttttt tctaaacgcg catatttttt aaaatttgct
    43561 tccggcgtaa actgatcgat caaaatcgct tctggttttt ctggctcgat tttttttagc
    43621 agcaaatgga tcgcttgatt gtgcaaagcg actttcatcc gaacggcatt gtattttggt
    43681 tggatctcat tgtatttttc tggagataag acaagcagtt tataggggat ggtttttttt
    43741 aagatgtcag caagctggat gatttggcta tcttttaact cttttgaatc tctaacacca
    43801 agtttacgta attctgcaag ctgatcacgt ttcacataag cagcacatac tgttagcgga
    43861 ccaaaatagc tgccatttcc gacctcatca ctacctaaaa cggataattg actgatattt
    43921 tttggcaaag aagaggctgt ttttgctggc ttggcttgtt tatcctgtgc ctccccccat
    43981 ttatcagctt ctattgctgc attcgtacct tgaaacatca cttttccgga tgtataagcg
    44041 gtaatggtcg tcccgtcttt ttttgcagca aagctagtat aaggaatctg tttatttaac
    44101 aaataagagc gatagtatgc tttcatttga ctaagttggt cgtttgttgc tttgataatg
    44161 acgttgctca taaaattctc ctcgttttta tacataaaac tataccgcaa ctcacgactt
    44221 ttcgttttct aagagattgc gagacatagc atttttttaa agtttatcat ataatagtta
    44281 agtatgtgaa aaatatttgt ggagaacggt gaaagaacca acttcatggt acactaaata
    44341 aaactttcat caaataggag gaaatgattc gtatggcgca cgaaaaaaca cgctacaaag
    44401 cagtgattgc caaccaaaca tatactatta tcggacggga aaccaagcat catatggata
    44461 tcgtaacgaa gctgatcaat gaacagttag cagaattgaa acaattatca ccacaaatgg
    44521 ataatgaaca agcagcaatc ttgatggctg tcaatgcgct ttcggatcaa ttgaagaaac
    44581 aagaaagaat cttggagctg gaagaagaaa cagcagaatt aaagaaaaaa atgatcaaat
    44641 ttacagagct tgaaaatcgt gtgaaacgca ttgaagcaat cgagaacgaa gctcgggaag
    44701 tattgaaaga aaatggtcaa gcagatcata ccattaaaaa tcatgtggaa gcacaacaga
    44761 ttttaaacga gaaacgaaaa ggacaaatta aacaaaaatc ttctaattaa gatcctttta
    44821 tcaaaataag aaaaatttaa aaaagttaga agagaaagga acgtagcatg ctgagtttgc
    44881 tgattttatt tttattgcta attgcgtttt tttctggagc acgtcgcggg tttgctttgc
    44941 aaatcatatt cgcgattgga tatgtattgt catttattgc tgcccagcat ttttacaaac
    45001 cattagcaag tcatttggaa ttatacattc catacccagc tgtcacccca acatcaaagt
    45061 tagcattttt tgatcaggta tttgcttttc acctagatga agctttttat gcaggaactg
    45121 cttttctgat tattttgcta attggctggc tgcttacccg atttgtgggg gtttttgtcc
    45181 atggattgac gtatgtgccg atcttgagac aggtagactg gattgcagga ggcatcctgt
    45241 cactaatcat ggcttacgtt actatatttt tgattttgca gttacttgcc tttgttccat
    45301 tagtgtggtc caaaatcaat tcggcgagca tagtttcgct cgttttatcg ttgagagaac
    45361 gccatttctt accaacaaga tgtatgagct ttgggtaacg aatattttga attaaggaaa
    45421 atagtgctgg aaaatatttc gttttttgta cagagtagtt tatttaagaa ggctgcgtag
    45481 cgtatgtccg gcacagctct tttttcatag tgaaagataa ggtgataaaa tgaataaccg
    45541 aatccttgaa acattggaat ttgaaaaagt caaacagatg gtcagacaat ttgtagtgac
    45601 tgcacaagga aaagaagaac tggcagaatt ggtaccagtg agtgagaaac aaacgatcac
    45661 aaactggctt caagaaactg aggatggatt gaaagtgcaa cgtctgcgag gcgggatccc
    45721 tatacctaaa cttgaaaata tccgtcccca tatgaaacgg atcgagatcg gagcagactt
    45781 gaatggagtg gagcttgcac aagtttccag agtattgtct acgacaagcg aattgaatcg
    45841 tttcattgat gatctttctg acagtgaaat cgaatttgca cgtttgtata tgtgggcaga
    45901 tcagttagtc acgatccctg ttttgagccg ccgtttaaag gaagcaatcg atgaagacgg
    45961 acgcgtgact gatgacgctt ctcctgagtt aaagagcata agacagaaca ttcggcgaag
    46021 tgaacaagct gttcgtgaac agttagatgg gatcgtccgt gggaaaaatg caaagtattt
    46081 aagtgatgcg atcatcacca tgcgaaatga ccgctacgtt atcccagtca aacaagaata
    46141 ccgcggtgta tttggcggtg tagtccacga tcaaagtgct tcaggacaga ctttatttat
    46201 tgaaccaaaa caagtggtgg atttgaataa tcgtttgcgt caataccaaa tcgctgaacg
    46261 aaatgagatt caacgtattt taagtgaact ttctgctgaa cttgttcccc atcgtcaaga
    46321 aatcatccac aatgcttatg tgattggtaa aatggatctg atgaatgcaa aagcacgatt
    46381 tggtaaagaa gtaaaagcca tcgtgccggg aatcagtgaa gacaatcatg tcgtcttgaa
    46441 acaagcaaga catccactga tcgatcagga aaaagtagtt tctaatgaca tcacaatcgg
    46501 aaaagactat caagccatcg tcattacagg acctaacaca ggtgggaaaa cgatcacgtt
    46561 gaagacttta ggtctgttac aactgatggg gcaagcagga ttaccgattc ctgcaggtga
    46621 agaaagtcaa atcggtattt ttgaggaagt atttgcggat ataggagacg aacagtcgat
    46681 tgagcaaagt ctatcgacct tttcttctca tatgacgaac actgtagata ttttatcaaa
    46741 agtcaatgaa aaaagtctgg tattatttga cgagctcggt gcaggaacag atccgcaaga
    46801 aggtgctgct ctagcaatcg caatcttaga tgatcttggt aaaaagtctg cttatgtgat
    46861 ggcaacaacg cattatcctg aattgaaagt ttatggatac aatcgggcaa atacgattaa
    46921 tgccagcatg gagttcgatg tggatacact aagtccaacg tatcgtttgc tgattggcgt
    46981 tcctggacga agcaatgcgt ttgaaatctc tagccgatta ggtcttgata cagaagttat
    47041 tgatgaagcg aagcagttga tgaatgatga gagccaagat ttaaacgaaa tgatcacaga
    47101 tttagagaat cgccggaaaa tggcagagac cgaatatctg gaaatgcgtc attttgtttc
    47161 tgaggcacaa gaacttcacg atgatttgaa agaagcttac agctatttct ttgaagaacg
    47221 tgaaaaagaa atggaaaaag caaagaaaaa agcaaacgaa gtcgtgtcag aggcggaaga
    47281 aaaagcagaa aaaatcattg ctgatattcg taaaatgcaa cagcagattg ggcaaggaaa
    47341 tgtgaaagag catcagttga tcgatgcaaa gacccaatta gcaaatctac atcaagaaga
    47401 aactttgaag aaaaataaag tattgaagaa ggcgaaagaa caaaaaacgt tgaaaccggg
    47461 agacgaagtg ttggttacga cttacggcca aagagggacg cttttacgta aaaacgggaa
    47521 tcagtggcaa gtagaaatcg gtatattaaa aatgaatgtg tccgaagacg agctgacgcc
    47581 tgttgctcct caaaaagagc cgacacaacg agttatccat gctgtacgat ctgaatctag
    47641 tagtcacgtg ccgaatcaat tggaccttcg aggaaaacga tacgaagaag cgctaagtga
    47701 ggtagatcaa tatttggatt ctgccattct agctggttat cctcaagtga ctattgtcca
    47761 cggaaaagga acaggcgcac ttcgcaaggg aattactgat tatcttaaaa accatcggag
    47821 cgtcaaaagc tttgagtttg caccggcaaa ccaaggcgga aatggtgcga caattgttaa
    47881 atttaagtaa agcttactaa aggtaagcag atggttcgct atccaagtat agaaatgcta
    47941 tactaatcat agattttaaa aatggaggtc tttgatatgt cacaagtaat tacagataag
    48001 acatttaatg aagaaacaga taaaggactt gtattgattg atttttgggc aacttggtgt
    48061 ggtccatgtc gtatgcaagc accgattttg gatcagttgg aacaagaata tgatgaagaa
    48121 gaattcagaa ttgctaaaat ggatgtggat gaaaatccag aaactcctca acaattcggt
    48181 atcatgagca ttccaacatt aatgttgaaa aaagatggtc aagtagtaga aaaagctgtt
    48241 ggtgttcatt caaaagaaca actacgtcaa atgatcgatc aatatttata aaaacttaca
    48301 gcaaaaataa gctgagggtg ggactgaatc cagttccgtc ctcggttttt ttgcggttga
    48361 gttaaggtta ttcaaattaa tattttttga gtataattac gttgtcaggc tggattgttt
    48421 ttttgacaga catgctataa taactttaat tgaatatgaa ggggagtacc agatgaacga
    48481 acgaatcaaa aacaaattag ctttacttcc tgatcagccc ggctgttact tgatgaaaga
    48541 taaaaatgga acgatcattt atgtcggcaa agccaaaata ttaaaaaatc gtgtccgctc
    48601 atattttact ggcagccata atacgaaaac ggaacggctt gttagtgaaa tcgaagactt
    48661 tgaatacatc gttacagaat cgaatatcga agcattgctc ttagagatca atctgataaa
    48721 gaaaaatgat cctaaataca acattatgtt aaaggatgat aaaacctatc ctttccttaa
    48781 gataacgaat gaaaaatatc cccgtttggt gattactcgt aaagtactaa aagacaaagc
    48841 actgtatttt ggaccatatc ccgatgtcgg tgcggcgaat gagacaaaga agatcctcga
    48901 tcgattattt cctctgagaa aatgcaaacc tagtcagacg aaggagcctt gtctttacta
    48961 tcatttggga caatgtctgt gtccttatta tttcgacgta gatccgaaag tctatacgaa
    49021 aatcgttgat gaagtgaaaa agtttttaaa cggaggacac gaaaaaatcg agtcagagat
    49081 ccatcagaaa atggaaaaag ctgctgaaaa catggagttt gaaaaagctg cagaataccg
    49141 agaccagatc cgtgccattg agacaatcat gactcgccaa aaaatgacaa atactgattt
    49201 actggatcga gatgtatttg gttatgcgat tgacaaagga tggatgtgtg tccaagtgtt
    49261 tttcgtaaga caaggaaagc taattgaacg agatgtttca atgtttcctt tctataacga
    49321 agcagaagaa gatttcttga cttacctcgg tcagttttat caggaaaatg agcatttcat
    49381 tcctaaagaa gtcttgattc ctgatacgat cgacaagccc agtgtagaag ctttgttagc
    49441 tacgaaagtg ctgcaaccaa aacgaggaga aaagaaaaaa ttagttgaat tggcgaataa
    49501 gaatgcctca gttgctttga atgaacggtt tgatctgatt gccagaaaac aggaacggac
    49561 gatcggagca gttgaacgtt taggaaacgc gatgaatatc ccgaccccaa tacgtatcga
    49621 gtcttttgat aactctaata tcatggggac gaatcctgtg gctgcgatgg tcgtattcat
    49681 tgatggaaaa ccggcaagaa aagaatatcg gaaatataaa ataaaaacag tcaacggacc
    49741 ggatgattat gcatcaatga gagaagtcat ctatcgcaga tattcacgag tgattcgcga
    49801 agggttgcct ttacctgatt tgatcttgat cgatggagga aaaggacaag tagatgtggc
    49861 aaaagatgtc ttagaaaatc aattaggaat cgatattcct attgctggca tggcaaaaaa
    49921 cgataaacat aaaacaaatg agttgctatt cggtatagat ttggctgtag taccgcttga
    49981 gcgaaattca ccagagtttt tcttactcca gcgaatccaa gatgaagtcc atcgattcgc
    50041 aatcactttc catcggcagc tacgcagtaa aaacagtttt gcttcccgat tagatgaaat
    50101 tgaaaggtta ggaccaaaac gaaaaaaaat gctgcttaaa gagttcaaat cgttgaaaaa
    50161 tatcacagca gccacgacag aagaattaca agaaatcgga ctgccgaaaa atgtcgcaca
    50221 aaacgtattt gataaattac atcaggaatg aggaaagggc actttacaaa gaaagtaaag
    50281 tgtcctatca ttttatgtac aacaaaaaga gcgtttacgt tttgttggta aataataaat
    50341 cgcttacgtc tggtgtctaa ggatatatgt gatttcttga agaaggagtg aagaaatgtt
    50401 agaagtcaag gatcttgtta aaacttttgg gaactacaca gctgtcgatc atgtctcttt
    50461 ccaaatcccc gatggaaaaa tcatggggct aattggccaa aacggggctg ggaagacaac
    50521 gacttttcgt ttgatcttgg attttttgac acaagaccaa ggagaagtgt tatggaatgg
    50581 ccatcatctt ggggagaaag attatgatgt catcggttat cttccggagg aacggggact
    50641 ttatccgaaa gtaacgattc aagaccaact gctttatttt gcagaactta gagggaaatc
    50701 aagaaaagag attgaaccaa aaatcgattt ttggatggaa aaatttcagg taaaagggaa
    50761 aaagacggat aaagtaaaat ctttgtctaa aggaaatcag caaaaagtcc agctgatcgc
    50821 cacgttaatc catgaaccta aattgatcat tttggatgag ccattcagcg ggttagatcc
    50881 ggtaaatgct gaattgctaa aagatggaat attggaattg aaaaaacatg gttcatgtgt
    50941 cattttttct agccacaata tggataatgt agaaaaaatc tgcgatcatt tgatcatgtt
    51001 gcgaaacgga aaaatggttt tgaatggaaa agtacatgag attcgtgaat catttggccg
    51061 tacgaagtta ttcttggaat ctggtttaag ccaacaggaa gtagaagaaa tcgaaggcgt
    51121 gaaaaaagtg atccttcatg aagacaatac gttagagatt accttagaag atcctgaagc
    51181 tgggaaagaa atctttactc gtgcgactca gttcggctac attcctatgt ttaaccagca
    51241 accgccaact ttggaagaga ttttcaaatt gaaagcaggt gaaatcgatg cgtaagtttt
    51301 ggattattac caaagatgtc tatttaaaaa atgttaagtc gatttctttc ttgatcatga
    51361 tattagtccc ttttatcatg ctgggtatca tttatgtagc aggaaacttt gctcaacaaa
    51421 atagtgatgt caataaaatc ggcgtattgg ctgataatga gcaaatcagt cagcaaatga
    51481 gccaagtcaa aacagatgat tttgagttca aagcagttga ttctgaagaa aaagcacaaa
    51541 aagagctttc ggaagaaaaa atcgatgcgt atttagtagt ctcaactgag aatgataaga
    51601 taaatgggat gctttatagc gaaaattcat taggacaaag tacacaacta ctgatacaac
    51661 aacaactgac tggcttgcaa tcgatgcttc gagcaagtca attaggcatt tcaccagaag
    51721 aagtagctag cttgagccaa caagcaagtt tgactcgaca aaaagtcaat tttgattcaa
    51781 aaggaaaaat gacagttgga gaagataact cgacagtcca atatgtcgta agttatgtag
    51841 caacgattgt cttgttcatt attattttga cgtatgcgca gatcattgca caagagatcg
    51901 cttctgaaaa aggcacgcgg atcatggaag tgatcctgtc cagtacgact gcacaaaagc
    51961 atttttacgg aaaactaaca ggtgttttac ttgttgcatt aacgcaaatg gcattatacg
    52021 gtattatttt tggtatagga tacagtcaat tcaaagatat ggaaatggtc aaaaatatat
    52081 tagacggtat ctcattggat aacttgattg gaccattctt gtggtattct cttctattca
    52141 tgatctttgg cattttgatt ttttctgttt tagcagcttt atgtggctct ttggtcaaca
    52201 aagcagaaga tacagcaaaa gcaattttgc cagtgactta tctatctcta ggcggatata
    52261 tgcttggatt gatactagga gcttcagatc caaacaatat cgttattcgg atcacttcgt
    52321 atattccatt cttatcttca tatattatgc cggttcgctt agcaaatgag acagtcggta
    52381 tgggtggagc attgatttcc ttgttggtac ttgtagccgt aacattctta ttgatgatct
    52441 tgtctgcaaa catgtacaaa tcgaatgtac tcgtgtatag cgaaggcgga ttatggagtt
    52501 cattgaaaca atcgatttca attatgagaa atgaacgaaa aaaagcataa aatatagaaa
    52561 aatatagaag gaagctggac atttattgtt cccagcttct tttttgtata gacctatttc
    52621 tattttttta ttatattgga tatatatatt cattgaaaat agagcataac aactttataa
    52681 tgttgagtat accaattttt ggaggcgttt taatggaccg ctatttagat ttactttcag
    52741 agaagttccc tacaacggaa gatgtcatca cggaaatcat caatctcgaa gcaattttgc
    52801 agcttccaaa aggaacagag ttatttttga gcgatataca tggcgaattt cctgcttttg
    52861 atcatattct gcgtatcggt tcaggaaatc ttaaagaaaa gatcaaagac cttttctctg
    52921 aacaaatgac ggaaacggag atcagccaat tcactatttt tacggcttat ccagaatatg
    52981 tgttgacgac tgattggtac aaaaaacaag ataaatccca gctgatccat cgtttgatcg
    53041 atcttttacg gtttacgaca gtcaaataca ctcgatcaaa agttcgaaaa ggattgccga
    53101 aagaatatag ctatatcatt gaggaactct tgtatatcga tgatcgtatc aacgggaaaa
    53161 aagactatgt aaaaaaaatc attgaacaac ttgtcttgat gaaggaagaa gaaaaatttt
    53221 taacaaaact tgcgcagacg atccaaaaat ctgtcatcga tcatttgcat atcgtgggtg
    53281 atatttttga tcgaggaact caagcagcca aagtaatgga tcgaatcatg gatttttcct
    53341 ctgtagatat ccagtggggg aatcatgata ttttgtggat tggtgcgtat tgcggctctg
    53401 aggcatgttt attgacgctt ttacggattg ccgcacgcta caactattta tttgaattag
    53461 agaaagaata tggattaaat ttacgtccat tattttcttt tgcccatcaa acctatcaga
    53521 agaatccagt atttacccct aaaaacagga aagatctatc tgaaagagag caagaagaat
    53581 tagaaaagat ccatcaagca ctttctatcc tccaattcaa atcagaacat cagttgttga
    53641 atcgacgacc tgaatttaaa atggacgatc gtaaattgtt ggaaaaaatc aactatgagg
    53701 aatcaacgat cgttttagaa ggtcagctct atgagttgaa agggacttgt tttcaaacga
    53761 tccagccaga tgatcctaaa aaattcttgg aagaggaaca aaaagtgatc gatagtttga
    53821 tgtattcatt ccagcactcg ttaagaatgc aaaaacacat gacgtttttg atcgaaaaag
    53881 ggggaatgta tctcacgaac aatcagcatg tactgttcca tggctgtata cctgtagatg
    53941 aaaaggggca gctcttagca tttgaattag accaatctta tcgcggaaaa gaactgctgg
    54001 gattttttga aaaacagata actgaaagcg caaaagattt gactgcaaat gaagatttgg
    54061 cgaccgattt gatttggtat gcgtggagcg ggccattttc gcctttattc ggcaagagta
    54121 aaatggcaac ttttgagcgc tattttctca cagagcctca tacgcacgtc gaaaaaagca
    54181 atccctatta tcacttgcga gatgaagagt ggttcagtga aaaaattttg gcagaattcg
    54241 atgcaaaaga agaaggttca gcaatcatca atggacacac gccagtaaaa gtaaaaaaag
    54301 gagagtcacc gatcaaagca aacggaaaag catttgttat tgacggaggt ctatccaaag
    54361 cttatcaaaa aacaaccggg attgctggct actcattact atgcaattct tacggtttcc
    54421 aaatcgtgac acattatccg ttcttgccta ttgaacaaca atttgcaaaa aaatctgatc
    54481 aaacgaatgt aaaaaaagtg atcgagcagc agctcccacg caaacttaat cggaatacga
    54541 caaaaggaat ggaattacag caacagatta ctcaactcca aaggctgttg gattatcgaa
    54601 aggacgattg agaaaaatgc tagccaacat tgctcaatag actaaaaagc gactgcaaca
    54661 aaaggcgtgt cgcagtctcc ttcggaatta gctcaaaaaa cggtaaaata ttagaagcta
    54721 tttttctgtt tatttctcta atactcaggt cagggcgctg atttcacgac ctcttcctta
    54781 aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatccaacaa acatgagaag
    54841 ctgttttttg aaattgctcc ttgtgactca gagttgaacg gcaggctcac gacctaatca
    54901 gaaaaacgat taaaattcat tcacaaagaa atcgaataaa cgctgttcag atgaatctac
    54961 acgatgagaa agttccggat gccattggac accaaggatt ttgtgcatat tatctttact
    55021 ttggactgct tcaatgagtc catcgttgga aaaggcgatg gcttttaaca aaggagaaag
    55081 atctttgact gcctgatgat gataggaatt cacaaagtat gaatcaggga gaaggtcact
    55141 taataaactg tccgaaacga ttttgacttc gtgagttgca aactgcgggg cagtgggctg
    55201 ttgttcgtgt tttacggacc attttggata taatgaaaga tcttgataaa gtgttccctc
    55261 caacacgaca ttcaatagtt gaagtcctcg gcagactgca aaaatcggtt ttctttgttt
    55321 caatgcttcc aagatcaatg cctgttcgaa ttggtctcgt tgaatatttg tttccgtcag
    55381 ttttggatga gggtcttcca tgtaaagttg cggactaaca tcctgaccgc ctgcaagaat
    55441 gagtttgtcg attttctcaa ttagttgagg cgctgaatct ggagacatga ctggcagaat
    55501 aagaggtaat ccttgagcat ctaaaactgc atccacaaat ccctgcggtg tatagcttac
    55561 ttgatttcct tggaaagtat ctgtcgctcg aatcagttga ttcccggcaa tgccgatgat
    55621 tggtttcatt tttattcccc ctaaagtaga ctaattgtaa caaggattta aaaaaattta
    55681 aactatgtaa gacttgcccc tatacttatc tgttcttgct gtgttactat ctgtaaagag
    55741 aattttcgtg tgaaattcat gacgatagtt gaaaatattg cttaatttgt tataataaaa
    55801 agtaagcaga aataaaaagg ttgggcgtaa gcttcaagga tctagttctt caaggggtga
    55861 gaaggacatt ttattgaagg ggcctgccta tactaagtta tctttagagg tacatacttt
    55921 caatgaaaaa agaaagcatg tacctctttt ttaaatcgaa aggaagtgaa gcaagtgtta
    55981 gaagctttgg aacaaatccg aaaagcagaa gaagaaaatg agaagcgtgc tcgaacccta
    56041 cggcagcagt tgaagaccta tgcagaagaa aaagagcaag aattggagat aatccaaacg
    56101 aacagtcggc aacagctgaa acagttggta gaagaaaaag aacaattcga aaagcaagaa
    56161 ttagaaaaag aaaaaatgaa attgaaagca gaagcagaca aggtgcaagc aactttgaaa
    56221 gaccaatatc aaaagcatca gagtcaagca atcggtgcaa tcattgaaag ggtgaaagaa
    56281 acttatggcc gtcactaaga tggaaaaagt gaccctcatt tcagataaaa gaaatcagga
    56341 gaggatttta caagcagtac aaggaatcca aaatgttgaa atccgggatc tgtatcaaaa
    56401 tagaacaaac aaccagtggg tagaaaaata ttttccagat accacgatta ttgataaaga
    56461 agcaaaatta agcgctttag gcaatcggct gaaagacatc caagaagcta tccaattcat
    56521 acaacaccac ggcgatagcc gtcaaaagaa actccatttg aaacgtcaag aactttcctt
    56581 acaagacttg gaagcaaatt attccgaaga aaatttcatg aataaattag aagaagtcct
    56641 tgatttaaaa gaaaagtggg aacagctaac tgaaaaacgt gatcgtttag aagaagagga
    56701 agaatggctt cttcattggc aacagctgga tattgctcca agtatgtatg caagtaagac
    56761 gaccattttt gagatgggga ctgtgagtgc agcaaatttt gaaagtttca aggaagaact
    56821 aagtaagctt gatgacgttt atatggaaga aatagattat acgccaaaac aagtttatct
    56881 ttcgtatgtt gctttaaaaa aatcagttgc tgctatagaa gaaattgctg ggaaatacgg
    56941 gttcaataaa gaaacgtatc cttatgaaca aaatcctaaa gaaaaattgc aagaaaccaa
    57001 acagcaatta tccattgttc acgagcagca aaaaaagata acgacagcgt taggtcagtg
    57061 cagcggctat atccaagaat ttgaatgggt agaagaagtc acacttgccg ttgcagaacg
    57121 ggaaaaagta aaagaacggt ttatccatgc cacttatctg atcgttttgc aaggttgggt
    57181 agatgcagaa gaaaaacaga atttgatgca tgtgttaaac gaaactgtat cagaagaaga
    57241 tatatacgtt agtttcgaaa caccaaactc agaagagatt cagcaggaag tccctactaa
    57301 attgaaaaac catccgctta tcgaaccttt tgaattgctg actgaaatgt acagtctgcc
    57361 aaaatatgaa gaagtagatc caacgccttg gatgacacca ttttatcttg tcttcttcgg
    57421 gatgatggtc gcagatgttg gttacggatt gctgatgctc attgggacaa tactagctca
    57481 aaaactattt gttttacccc gcgggatgaa acgtttcgta aaattctttg agattctctc
    57541 ggtaccatcg atcatctggg gattgatcta cagttcattt ttcggacagg cattaccaaa
    57601 agaactattt ggtatccgct tgcctttccc aatcctatct acgacggatg acgtcaatac
    57661 gattttgatt ttatccgtga ttttcggtct gatacagatc ttggttggat tattcgtggc
    57721 agcaaaggaa aatatcagga ggaaagatta cttgagtgct gtcagtgatg gttttgcctg
    57781 gcaagggatc ctgattggga tcgttattgc attgattggt gtcatgttga taaaaaatac
    57841 agcatttgtt tatcttggcg cggggctatc gatcattgct gctttatgta tcttaatcgt
    57901 gccgattatc caatcgccgt ctaaagtcaa gggtgctgcc aaaggagcgt ataatcttta
    57961 cggattgact ggttatatcg gtgatttagt cagttataca cgtttgatgg cactaggtat
    58021 ttcaggcgga agtattggcg cggcattcaa tatgctagtt gctttcatgc cgccagccgc
    58081 tcgattcagt gtagggatcc ttttgatcat cgctttacac gcactgaata tgtttttgac
    58141 actgttaagt gcctatgtcc atggtgcacg attgcagtat gtcgaatttt ttggaaaatt
    58201 ttatacagga ggcggacgtg cctttgaccc gctgaaaacg gcggaaaaat acgtcaacat
    58261 caatcataag aaaaaaaaca ataaataatt ggaggatttt tatacaatga tggattactt
    58321 aattagtcaa aatggtggaa tggtatttgc agtattagcg atggcaacag cgacgatttt
    58381 ttcagggatt ggttcagcta aaggtgttgg aatgacaggt gaggcagcag cagcgttaac
    58441 aacaagtcaa cccgaaaaat tcggacaggc tttgattctg caattgcttc caggtacgca
    58501 agggttgtat ggattcgtta tcgccttttt gatttttatc aacttaggaa atgatatgtc
    58561 agttgtacaa ggattgaact ttctaggcgc ttctctgccg attgctttta caggtttgtt
    58621 ttcaggtatc gcccaaggga aagttgcagc agcaggtatc caaattttag cgaaaaaacc
    58681 tgaacatgct acaaaaggaa tcatttttgc tgcgatggtt gaaacatatg cgattttagg
    58741 tttcgttatt tccttcctat tagtattgaa tgcctaagca taaaggagga aaagcagatg
    58801 aacgccattg aaaaaatcat ctctcagatg aatgaagcag cagagcaaga aagagcggcg
    58861 ttagagcaag aagagcgaat gaagatcgat caaaactttg aacaaaaacg tacgcaaatc
    58921 gaaacagaac atcaaaaaca aaaagaaaaa caaatcgaat tacttgaaaa aaaataccgt
    58981 cagcttagga atcgccaaca agtagaagta cgacaagaaa atttgaatgc gaagcaggaa
    59041 tttttacgac gattgtttgc agatgcaata gctgaaatgg agaattggga tgaatctgaa
    59101 caaatccagt ttatcaaaaa ttcattgtat tcattacctt tgacaggcaa agtaacattc
    59161 attgctggag aaaaatcagc agcttatctt tctcagacat tgctggatga atggaacaat
    59221 gaacttccct ttatgatggt tttaagtgat gaaactgttg ctgatcaagc gggttttctg
    59281 ataaacgatc aaggagttca gtataatttt cttttcagca gccttgtaca agatatccaa
    59341 ggaacgatga gttttgagat tgccaatcaa ttattcgaat aaggagggat cagtgtatgg
    59401 aataccacga actaaatccc ttgattcgcg gaagagagct tgaacttttg tccaatgaga
    59461 catttgaacg gatgattcaa acaaattctg tcgaagcact tggtgaaata ttgaaatcca
    59521 cgatttacag tccatatatt tatgacggat atgaatatga cttcgaacag aatcttttaa
    59581 aggagcagag ccgtctattt cgctggttaa aggaatcagc accggagcct gaaatcgttt
    59641 ggatctatac gatgcgctat actttccata atctgaaggt tttgacaaaa gccgagatga
    59701 caaatcaaaa tctggaccat ttgtatatcg acgatggatt ttattcatta gaaaccttga
    59761 aggatgccat tcatacgcaa gtttcaaatg aactccctga aagcattatg gacagtatac
    59821 gagaagtacg tgattatttt gaagagtcta ccattcttca gggaattgac gtgatttatg
    59881 atcgacagtt tttgacagag cagcgacggc ttgccgataa gctaggatat caagaattgc
    59941 tgacagaagt cattgctttt attgacttga ccaatatcac aacgatggct cgaggaatat
    60001 tgcagaaaag aacatatggg ttcatgacga cggtactttc cagctcggga agtatcgaaa
    60061 aagaagtatt tttgaacttt gtagataaag atatggaaga ttacacgaat tttctattag
    60121 agacagatta tgctgatttt ctgcgccctg tcatccgtga gggaaatctt gatctcgtaa
    60181 agcttgaaca gttgaaagat gattatctaa gttcattgta tcaagaagca caaacccagg
    60241 ctttcggacc actgccacta ctagcctttt tgaacgcaaa agaggtagaa agcaaaaacc
    60301 ttcgtttact ggttatcggc aaaagaagcc atttcgaacc agaaacaatc agaaagcggg
    60361 tgagacaagt ctatgacgca taagatcggt gtagttggcg acaaagattc tgttttgcca
    60421 ttcaaattgt ttggctttga tgttcgttat ggtacaacca agcaaaccgt tcgtcaggca
    60481 attgaagaaa tggcaaagaa tgagtatggc gtgatttatg tcacagagca atgtgcagca
    60541 ttgagcccag aaacgatcga acgttacaag gaacaaatga tacctgctat cgtattgatt
    60601 cccaatcatc aaggatcatt agggataggg ctggaagaaa ttcaaaaaaa cgtagaaaaa
    60661 gcagtaggtc aaaatatctt atagtatgaa aaggagatag aaattttgca aattggaaaa
    60721 atcataaaag tctccggacc tcttgtaatg gctgagaaca tgtctgatgc cagtatccaa
    60781 gacatgtgtc tagtgggaga tctaggagtt atcggagaaa ttatcgagat gcgtggcgat
    60841 gtggcgtcta ttcaagtata tgaagagact tctggaattg gtccaggcga accggttcgc
    60901 tctactggtg aagctttatc tgttgaatta ggaccaggga tcatttcaca aatgtttgac
    60961 ggaatccaac ggccattaga tacctttatg gaaatcacac aaagtaattt cttagggcgc
    61021 ggagttcaat tgcccgcatt ggaccatgag aaaaaatggt ggttcgaacc aacagtagaa
    61081 gccggagaaa cggtgagtgc tggagatgtt atcgggatcg tagaagaaac aaaagttata
    61141 aaacataaaa tcatggtgcc aaatgggata aaaggaacga tcaaaacaat cgaaaaaggt
    61201 tcattcacta ttgatgaaac agtatgtgtc attgaaaccg aaaatggtga caaaaatttg
    61261 acgatgcttc aaaaatggcc agtaagacgt agtcggccaa tcaaagaaaa attaaacccg
    61321 gatgcaccca tgatcactgg acagcgagtg attgatacat tcttcccagt gacaaaagga
    61381 ggagcagcag ctgtacctgg tccttttggt gcagggaaaa cagtcgttca gcatcaaatt
    61441 gcaaaatggg ccgatgtgga catggttgtc tatgtgggat gtggcgagcg tggaaatgag
    61501 atgacggatg ttttaaatga attccctgaa ttgattgacc ctaatacagg agaatcgttg
    61561 atggaacgta cggtattgat tgctaataca tcaaacatgc cagtagcagc tcgagaagct
    61621 tctatctata cgggaatcac aattgcggaa tatttccgtg acatgggtta tgacgttgct
    61681 atcatggcag actccacttc acgatgggca gaagcacttc gtgagatgag cggacgtttg
    61741 gaagaaatgc ctggtgatga aggttatcca gcttatcttg gttcacggct tgcggaatac
    61801 tatgaacgat ctggaagagt cattgcatta ggatcagaag gtcgcgaagg aagtataacc
    61861 gcaatcagtg ctgtatcccc gtctggaggg gatatttcgg aaccagtcac acaaaataca
    61921 ttgcgggtcg taaaagtatt ctggggtctt gattccagtt tagcccaaaa aagacatttt
    61981 ccttcgatcg attggataca aagctattct ctttattcaa cagaagtagg gaagtatatg
    62041 gatcatatcc ttcaagctga ctgggcatcc atggtcaccg agggcatgcg tattttgcag
    62101 gaagaacagc agctgaatga aatcgtgcgt ttggtaggga ttgattccct ttcagataat
    62161 gaccgtctga cattagaagt ggcaaaatcc attcgtgaag attatttgca gcaaaatgct
    62221 tttgatgatg tcgatacatt cacttcaaga gagaaacaat tcaaaatgtt gaagatgatc
    62281 ttgacctttg gtgaagaagg acgccgtgcg ttgtcgttag gtgcttattt aagtgagatc
    62341 atggacggta ccgtatcgat tcgtgaaagg atcggacgaa gcaaatatat cccggaaaca
    62401 gaattagaaa aactagatca aattattggt gaaatcaagc aaacgatgca aacgatcgtt
    62461 tcagaaggag gcatgagcaa tgattaaaga atatcgtacg atcggtgaag tcgtcggtcc
    62521 attgatggcg gttgaaaaag ttgctggtgt caaatatgaa gaattaatcg aagtccgtat
    62581 gcaaaatggc gagattcgtc gtggacaagt attggaagtc caagaagata aagcaatggt
    62641 ccaaatattc gaaggaacaa gtggtatcaa tttacgtgac tcttctgtac gatttttagg
    62701 ccatccttta gaactaggtg tatcggaaga catgatcggt cgagtattcg atggtttggg
    62761 acgaccgaaa gataacggac cagagatcct gcctgaaaag catttggata ttaacggaga
    62821 agtaatcaat ccagtagctc gggattatcc agatgagttt atccaaacag gtatctctgc
    62881 aatcgatcat ttgaatacat tagttcgtgg gcaaaaatta ccagtatttt ctggctctgg
    62941 tcttcctcat aaagaattag ctgctcagat cgcgcgacaa gcaacggtac tcgattcatc
    63001 agatgatttc gccgtagtct ttgctgctat tggaatcact tttgaagaag ctgaattttt
    63061 catggaagat tttcgtcaaa cgggcgcaat cgaccgttct gtcatgttta tgaatttagc
    63121 aaacgaccct gcaatcgaac gtatcgctac tcctcgtatg gctttgactg ctgcagaata
    63181 tcttgcttat gaaaagggaa tgcatgtttt ggttatcatg acagacatga ccaactatgc
    63241 agaggcattg cgagagattt ctgcagcacg gcgagaagtt ccaggaagaa gaggatatcc
    63301 tgggtatctt tacacgaact tagctacttt attcgaacga gcaggaagaa tccgcggact
    63361 aaaaggatcg gttactcaaa ttccgattct aacaatgcca gaagacgata aaactcatcc
    63421 aatccctgac ttgactggtt atattacaga aggacagatc atcttgacta gagagcttta
    63481 caagagtggg attcagccac caattgatgt actcccgtct ctttcgcggt taaaagataa
    63541 gggaactgga cccggaaaga caagagaaga ccatgcagct acgatgaatc agctttttgc
    63601 agcctatgca caaggaaaac aggcaaaaga attagccgta gttttaggtg aatctgcttt
    63661 atctgatgta gacaagatct atgcgaagtt tgctgagcgg tttgaaaaag aatatgtcaa
    63721 tcaaggattc tacacgaacc gttcgattac tgaaacactt gatctagggt gggaactgct
    63781 atcgatgttg ccaagaaccg aattaaaacg aatcaaagac gacatgctgg acaaatatct
    63841 gccagaaggg aagtgagttc tgatggcacg attgaatgtg aatccgaccc gcatggaatt
    63901 gactcgattg aaaaaacagt tgacaactgc cacaagaggg cataaattat taaaagataa
    63961 gcaagatgaa ctgatgcgtc aattcatcct gttgattcga aagaacaacc agctgcgtca
    64021 agaaatggaa gagtctatga cagaagcaat gtctgatttt gtattggcaa atgcttcgat
    64081 cacggaacct ttcatcgaag aattatttgt tttgccggcg gaaaacgttg atttgtctat
    64141 cgtagacaaa aatatcatga gtgtaaaagt acctgtgatg aattttgaat atgatgaaca
    64201 gctgacagac acacctttgg aatacgggta tttgaattcc agtgccgaac tggatcggtc
    64261 aatcgatcgg tttactaccc tgcttccaaa acttttggaa ctgacagaaa tcgaaaaaac
    64321 ttgtcaactg atggcgagtg aaatcgaaaa gacacgtcgg cgagtcaatg cattagaata
    64381 tatgacgatc cctcaattag aagaaacgat ctattatatt cgtatgaagc tggaagaaaa
    64441 cgagcgcgca gaagtaacaa ggttgatcaa agtaaaaaat atgggtacca ctgactaagt
    64501 ggtatctttt tttaaaagag gaaaacgatg attaaacaaa gcaaacaaat aaagaaacgt
    64561 ttgccggctg ttcagcttct cgcattagga ttttttctcc tgattttagt tggcggcggc
    64621 ctacttaccc taccattttt tagtaatagt gggcatggga caaattttat cgatgcgctt
    64681 ttcactgcaa cttctgctgt ctgtgtaacg ggattgacaa cattgaatac agcagagcat
    64741 tggaacagtg ctggtcaatt tttgatcatg cttttgattg agatcggcgg tcttggtttt
    64801 atgatggttc cgattctatg ctttgcattg ataaagaaga aagtcagtct cagcacacgt
    64861 attatattaa aagaagcgct gaatctagaa gaagtatcag gtgtgatgaa gctcatgctt
    64921 tacatcctaa aattggcctt tgttatccaa ttgattggta cggttgcttt atcttttgta
    64981 tttgtgcctg aatttggctg gttgaaaggt atctggtaca gtttgttcca tgcaatctct
    65041 agtttttgta atgcagggtt tgatttgtta ggagacagtt tagccaatca ccagcaaaat
    65101 gtttatttga tcatggtggt atctgctttg atcatttctg gtgggttagg cttcatcgta
    65161 tggcgtgatt tactggaata tcatcagaaa aagaagatga cgattcattc aaaaatcgca
    65221 ttaaccgtta caggtaccct tttattaggc ggattcgttg tgttcttttt cacagagcgc
    65281 aatggggcaa cgctagtgga agggaatatg tttcaacgat tggcaaacac cttttttatg
    65341 agtgtgacac ctagaacggc tggatattat tcagttgatt acctccatat gagccatgca
    65401 ggtctgattt taacgatgtt tttgatgtac atcggcggaa catctgggtc gacggccggc
    65461 ggattgaaaa caactacgtt aggtgtgttg ctgatccaga tgcgttcgat gttgaaagga
    65521 cgtacgagag cggaagcatt tggccgtacc atccgacaag gcgcagtgtt gcgggcactt
    65581 actttatttt ttgttacgtt gacgttgtgt gtagtcgcca tcatgatttt atcggtgact
    65641 gaaacgatac ctaaaacttc tggcatcgaa tatattgctt ttgaggtctt ctctgcgttt
    65701 ggtacagtag gtcttacgat gggattgacg ccagacttga ctgcaatagg gaaattgatc
    65761 attatagcgc ttatgtacat tggacgagtt ggtgtattga ctgtggtctt ctctctgatg
    65821 acaaaagcac aaagaacaca atcaaaatac aaatatccag aagaaagtgt catgattgga
    65881 taatttcatc ttctgacaga aacctagtga tttagtaaaa taagaaaata aaataatttt
    65941 tcttgcttct tggatacttt aagtgcaaaa tgttcgttta ttttttgagg cactacccgt
    66001 tgttgaacct cgggtagtgc ctctttttta ttgaaaggaa tcaataagat ttgattcctt
    66061 tatttatcaa taggttataa ttcatatttt tttatttttt cttttacgat acttagtcca
    66121 gaagctaaaa tttcaaggtt attagaggat agggaaattc gaacgaatgc atctggttgc
    66181 gcacgtgtag taaagcgtct ggaatgataa agtcggatac cgttagcaag aaagtcattc
    66241 tcaattatct tagggtcaat ctcaggatca actggaagaa ttcgatagta agggacacga
    66301 ttcgttggtt tttctacttt gaaaagagaa tcaaaaagct gattggctct ttttgtaaga
    66361 cttaatttag tttctaagat cctatttgct tcatttgaat ggaggatttg agtgatgact
    66421 tccacatcta atccagaagt tttcacattt atattaaaaa ttgcttgttc tatcttgctt
    66481 tggacatgta ctggaactac aagataagca actcgcaatc cagaacaaat aaattttgtc
    66541 attccagcta agtagattgt ttggttaggt aataaatttt ggaaaggagg aatgatcgta
    66601 tgttgatagt aagttgttaa aaatgaatgg atatcatctt caataaccca tagaccttct
    66661 ttttcaatca cttcggctaa ttgctctcgt cttttgtgag agatttgaaa ccctatagga
    66721 ttattacaag aaggcataag aaagacacca tgaatcttct tttttctaca ttcctgaatc
    66781 aatatatcag gtcgcattcc ttgctcgtca aaatcaattg gaacgatctc taaatgatat
    66841 aactgagcca attcaatgaa attagcgtat gtatatcggt ctgttgctat tctatcacct
    66901 ggagagaaaa gtgcagaaag tgtgatagcc aatccatttt gaacaccgga aacaatagat
    66961 agagattcta aggacggaat gactccttga gtttttaacc aatttgctgc agtagtcaat
    67021 tgaaaatttg tgcctttggg atcttggtaa ttaagcaaac caacaagatt tgggttatta
    67081 ctagttgatt gaataaacgg gacaacgaat ttattacatt cttcaaaaga actaaccaga
    67141 ccaaaatcaa tgatatgttc ggttacttca ttggtagaga tggtgatgga ctcaaagaca
    67201 ttgggagaaa taaatgtgcc acttccaata ttcgtataca gcaaaccttt ctcaattcca
    67261 tatttataag ccttgccaac ggtagtaaaa tttaaatcta aataatcagc tagctctctt
    67321 tgggagggta actgagtgtt tttctgtaat ttattcttta aaatatcttg ttctaattgt
    67381 tgaatcaacg atcgataata aggacgcttc aatttttgtt tgtctggtcg ccaagttaac
    67441 gggtaattgt cataagaatt aataggcatt ttctccactc cttaaaattg tataccatac
    67501 aattttacca ttgattgtat tgattttaga agttaatctt ttgtgaggag ggaattgtct
    67561 atgaaaatta ttaattttga ggaagtagta gcttctttta gttttgaaga agcgattcaa
    67621 gtaatgaaaa aatgtttttt tgattatgaa agtggtaaaa tcagccaaaa tcctcgattg
    67681 gtggaaattt tacctgacgg aaaaggcaaa aatatgtttg cgatgatgcc tgcttatttg
    67741 ggggagaaca gatattttgg cacaaaagtc attaccgctt ttccggataa tcatcagtat
    67801 catttacctt ctcatttagg agagattttc ttgtttgatg ctcaaaacgg actacccaaa
    67861 gcattgataa acgcaaatgc agtcacctgg attcgaacag ctgctgtatc tgcgctggca
    67921 acagattact tagcaaatcc gcatgcagaa acacttgctt taattggagc tggtcaacaa
    67981 gcattgtcgc atttgactgc gatttctacc attcgtccca tcactacagt gtttgtttac
    68041 gatattttag aagaaagacg aaatactttt atcgctaaag ctaaaaaaca gtatcctaaa
    68101 ataacgttta taaatgcaat atcagtagaa gatgcagtac gtccagcgga aatcatttgt
    68161 acgttgacct ctagtaaaga ggcatttctt caaaaagagt gggtaaggga agacgcacat
    68221 atcaatgctg tgggcacatt tactcctgat acaagagaga taagtacatc gttgatggaa
    68281 acttctcaaa tctatgtaga tgattactca gctgccttaa cagaaagcgg ggacctgctt
    68341 atagccatgg cagaaggaga tttagaggaa tcttgtattg ttggtacatt aggcgaatta
    68401 gtttctgaga aaaaaacatt ttataaagga gaatcgaggc cgacgatttt cgacgcagtt
    68461 ggtctagcta ttgaagatct ttgttgtgca gaatatattt ataacaaatt agagaaaggg
    68521 gctttaaaat gaacttagat tattacgtag ttgatgcatt tgcagatgag gtgttcaaag
    68581 gaaatccagc agcggtttat gtgctagaag aatggctgcc agaaggtacg atgcaaaaaa
    68641 ttgccattga aaacaattta tctgaaacag catttacagt gaagaaaaac caagaatttg
    68701 agcttagatg gtttacgcca gatcgagaaa tcgatctttg tggtcacgcg acattagcaa
    68761 cagcatttgt tttatttaac tattacaaaa tacctgatga aacgattaaa ttttctactc
    68821 aaagcggtaa tctatttgtg acaagaatta aagattatta ttacatggat tttcctagta
    68881 ttatgccgaa aaaagtgccg atattgtctg aatatgaaga agcgattggg gctaagatta
    68941 aagaagccta cttagcacgt gatttatttt tcgtattaga agatgaaaaa acggtggcta
    69001 aattgcagcc agattttaca gctatcaaaa attttgaatt aggaatagga gtcatcgtga
    69061 cagcagaaag tgtgcaaaaa gactttgttt ctagaacctt ctttccaaaa ttaacaatca
    69121 atgaagatcc agtatgtgga tcagcacatt ctaatcttat tccttattgg gcagaaagaa
    69181 tgaataaaac aaaattgtca gcttatcaaa tgtcatcaag gggcggggat ctagaatgtg
    69241 aattaaaaga agatcgtgtg ataataggag gaaaagcagt tttgttttct aaaggtacct
    69301 cgtttatttg atttctttca aatgaattaa aaataaattg gtggagagtg gcaaccttat
    69361 agctacaatg gttgttaaaa aaagacgcaa actttttcaa tttaatgaaa agtttgcgtc
    69421 tcattttaat tattggtgtt tgttttttat tcaatcaaga gtaaaatgcc ccatactaac
    69481 tatcctcgga tgacttcgat tttataacca tcagggtcaa tgacaaagta gtaagaaggc
    69541 gcagtgccag gtaatccttt taagtcagta atctcaagac cggcagcttt atgttgctcg
    69601 tgtaattttt ctagatcatc agctgctata gcgatatgac catagccgtc tcctagatca
    69661 tacgcaccat gatcatagtt gtaagtcaac tctaattcat agtcatcccc aggaagtgtc
    69721 aaataaacaa gtgtaaattt gttttctgga aaatcacggc ggcggctttc ttcaaaacca
    69781 aatgcctttt gataaaattc gattgatgct tcaagatctt tcactcgaac acatgtgtga
    69841 gccattttca tagattcatc tctccttttc cttttactca atcataacaa aaagaagtaa
    69901 ggaaatagtt gatttgtgct tgttcatgag aaatttctca tgtaaaatgg atagaaaata
    69961 aaactgttaa ggtggaaact attcatggat aaaccaattt ttggtgaaaa aaagcaattt
    70021 gctgattatc aaacccgtta tgcagcatat attgtcatag aaagagatca gcagattgca
    70081 gtaatcgagg ctccaaatgg tgcttttttt cttcctggcg gggaaattga aggagaagag
    70141 acgaaagaag aagcgattgt ccgtgagctg atagaagaaa tgggaattgt ggcagaaatt
    70201 tcttcttatc tgggagaagc tgatgagtat ttttactcga attatcgtgc aacttattat
    70261 tataatccag gatatttctt tgttgctgat tcttggaagc gtataggaga accaactgaa
    70321 aagaccaata aaatttggtg ggtgagccct caagaagcgc ttgtgaaatt gaaacgtggt
    70381 agccaccgat gggcagtcga gagatggatc gaaaaaaata aaaactgaaa gaaagcgagt
    70441 agatcagctt tctctcagtt tttttattta ttacagtaca tattttttga ttgcttcagc
    70501 tacaccgtgc tcgtcgtttg atgaagtgat gacatttgct gcttctttga caagagaaac
    70561 ggcatttccc attgctactc ctagaccagc atattcaatc atggataagt cgttctcgtt
    70621 atctccgata gccatgattt catcttgatc aataccaagg tgctgtgcga gattcgcaac
    70681 tgccgcacct ttacttgctt ctttatttag tacttcgaag taatagggcg cacttttgac
    70741 cgttgtgtat ttatcacgga aagtttgcgg taaacgcgca attgctgcat ctaaaatttc
    70801 tggttcgtcg atcatcatca ttttgacgat gttcatgtct ggtgtcattt cttcaggtgt
    70861 tcgatatttt agcggcatct tgactaaact tgcttcgtgg atcgtataag cgctgatatc
    70921 tcgattaggc gtgtagatcg ctttgtcatc gatcgaatgg agatgactgc cgacacggcg
    70981 tgccatcact tcgatctcta ggaaatcttc atgtgtcagt ccgtgacggg cgatgatttt
    71041 acctgatttc gtttgttgta cgagtgcccc gttataagta ataacgtaat cattatcttg
    71101 aaaaaggttc aactcgttta actgttcctg gacacctggt aagggacggc cagtgcataa
    71161 aacgatcgaa acaccttttg caacagcagc ggcaatcgct tcttttactt cttttgttac
    71221 ttctcttttc tcatttaata aagtaccatc gatgtcgata gcgactagtt tgatagacat
    71281 aatttcctcc aatatatttt aatttatttc aatcaatgcg ccattacgga tatggctggc
    71341 aaattcttga taagtttcgt caaataaatc ataatgatca cgcaaggacg gatcaaccat
    71401 ttcttttgga aagaaaaatc gctcatctcc ttgttcttgt cccgctaatg cagcaacaag
    71461 ctgactaacc tgtgataatt ctatcaaagt tccgtcattt cgcaaaatct cgatttgtgt
    71521 gcgatgtctg ccttgttctg ggcgataaaa atcataaggt agatcgtagc tagaattcac
    71581 agcagtgtaa taaacaggat tatatccgac cttttctact aacaaagtga gttcttggac
    71641 taattttgaa tcccggttgc cagaaaaagt agcagatttc agtggtttac gattcaaaaa
    71701 acgtttagct aaatcattta aaataggatc agcagattca gaccattgag taaagtaagt
    71761 gcccaagacc ccatcatcaa gtttgaggta ttcttctaaa gtaaagcttc cgtcaagaaa
    71821 tggcagaagt aattgagaat ggagttcaaa actgctggca tcttcttgat acaactcgtg
    71881 tgcgcgatga agcagatgct ccaaaattac ttccatacca cgggaaacag gatggaagta
    71941 cacttgcaca tacatttgat atcggctaac gatataatct tccactgcat gcatgccact
    72001 catggaaaaa gcgatcccgc cttcataagg acgaatcaca cgtaatattc gggtaagatc
    72061 aaatgttcca tattctgttc cagtgaaata agcatcccga agcaaatagt ccattcgatc
    72121 tgcatcgatc tgactagaaa tcatttggac aacttgtggg ttggggtaag ttttttggat
    72181 cacactagct accttttcag gaaaaccttc ttctactcgg ttaagaatct gatacacttc
    72241 tgtttctgga gaagtgatga tctctacagt gatggcttca tgattcgtat ggaagatatg
    72301 ttcaaatgta tgagagtaag gaccatgacc gacatcatgc aacagtgccg cacataaagt
    72361 caccagccgt tcctgatcat cccagcctcc gttgcctatt ttttcttttg aaaaatttcg
    72421 agaaaagata tcgcagattc gtcttgaaat ctcataaacg cctaatgaat gagtaaaacg
    72481 gctatgttct gctccgtgaa aagtgaaaga agaagtgcct aattgtttaa tgcgtcgtag
    72541 acgttggact tctttcgaat tgatcaaatc taagattact tgatgctgga catggatata
    72601 gttatggact ggatcacgaa aaactttttc cataggcagc atttgatatt tataaggggt
    72661 tgtcataatg tagctccttt atgttattac gttgttccat tctctggatt tggttgttcc
    72721 attgctccag ctggatttct ttttttagcc agtcggtaga atcctgtaaa tcacttgcag
    72781 aatacagctt ttcaaaagct tttgcaaatg cttctttgac ttcttttaca aaaaggggtt
    72841 ggtcaagcag ttcttcaagt gttgccattg tagcaggatc cactgcagga tagccatttg
    72901 taccaaagtc ttcttttaaa gcagattgat aaaatcgctt catgacttgc ccgcgttttt
    72961 cctgattacc gttgacactt aagtacatca taacagaaat cccatttttg atccgacgct
    73021 gagcgatacc ggcaaatttt ttgccatgga tgcttaaatc ataagttcca ggacaataag
    73081 aatgggtgat ttcgaatgct tgtatcggtg cctttggaaa agcgtattta gtcaatagga
    73141 gcatttgctc gtaagcgtca tctatggtta ttttttttgt gtcactttgg gggaaaatca
    73201 aagaaatatt caaaattcct tcatctgata cgatccctaa tccacctgaa tttctgatca
    73261 ctggagcgta acctgcatct ttcagtgttt ggatcccgtc acttagaaaa ggagttcgaa
    73321 catccttcat acctaagatg actgtgcagt catattgcca gaaatgtaaa aagatttgct
    73381 ggtgttcttt agaatattcc gttaatatat ctgttaatgc aaaaggggaa aagtcgtttt
    73441 tttgataaat gtgctgatct agtcgaatcg cagggttcat tcaaccggct cctttgtata
    73501 aattcatatt ttctacaata ttatactcta aaatcaaaga gttgttttgg aaaaaaggtt
    73561 ttctgtaaaa atggacaagt tggttgacaa aacaaaagaa actaggaaca atgtagctat
    73621 gagaagtaaa ggagaatgaa taatggaatt atcacaagga acaccgattt caatcaaatt
    73681 gcgtacaaaa gtaaaacagc aaggggaagt gcaagatttt tattttgatt tgaaagggca
    73741 aatggtgaca attggcgata cgctgtacat acgttataaa gagattcagg aggagacggg
    73801 cgatgagatt cctgtaacga tcaaattagt tccagatggt catgtacagc tgatccgtgc
    73861 aggagaaatg cggatgcgtt tgaaatttgg ctataaagaa cggttagaaa catcctatcg
    73921 aacaccatac ggtatgatcc agattgctac ttttacgaaa gaactgcatg ttagtttgaa
    73981 agaccgtccc acaggaggga aggttcgaat cgattatgat ctgttcatgg gaccggaaaa
    74041 aatcggcgaa tattatttga ccttggattt tacagcgtaa atataaaaga aaagtttgat
    74101 tttttgtgtc tgattttgta tgataaggag tagactgtga aaggacgtgt cctgattgga
    74161 aattagtgta tttgaaggtg caaacaaaag cgaattatca atgatcgaag tagcacacgc
    74221 gatcttagaa caacgtggcg atgtcatgga tttttctgat ttagctaacc agatccaaaa
    74281 ctatcttggg aaatcagaca gtgaaattcg tgattcactc gcacaatttt atactgactt
    74341 gaatattgac ggcagcttta tctcattagg ggataatcgt tggggcttgc gttcatggta
    74401 tgctatcgat tcgatcgatg aagaagtaaa ccatggaatg gacgaagagg atgaagatac
    74461 tccacgtcgc cgtaaacgca agaaagtcaa cgcctttatc aatgatgatg aagacgcgat
    74521 cgattacaat gacgatgacc cagaagatac agatctgaca gaagaagatg acgatgatct
    74581 gtttgatgac gatgatgatg aagatgaaga aatcgctgct tataattctg acttgcaaga
    74641 aatcggagca gatgacccga cagatgatga agaagacttg ccaggaaata tcgaagaaga
    74701 tttaagtatc atagacgaag acgatgatga tgaagattat gaagaggaag aattttctga
    74761 ctctgaaaaa gaataaacat tattttctta cgagaggttg tgacagagac gatcagccta
    74821 tcagaataag gaggtgtgac acgat
//
LOCUS       DAYYFU010000008        61538 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000008 DAYYFU010000000
VERSION     DAYYFU010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.008"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ACHA34_000657"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ACHA34_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFM8791329.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2944..3378)
                     /locus_tag="ACHA34_000658"
     CDS             complement(2944..3378)
                     /locus_tag="ACHA34_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791330.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3395..4600)
                     /locus_tag="ACHA34_000659"
     CDS             complement(3395..4600)
                     /locus_tag="ACHA34_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFM8791331.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5294..5680
                     /locus_tag="ACHA34_000660"
     CDS             5294..5680
                     /locus_tag="ACHA34_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8791332.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5774..5965
                     /locus_tag="ACHA34_000661"
     CDS             5774..5965
                     /locus_tag="ACHA34_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8791333.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6479..6811
                     /locus_tag="ACHA34_000662"
     CDS             6479..6811
                     /locus_tag="ACHA34_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8791334.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            6721..6915
                     /locus_tag="ACHA34_000663"
     CDS             6721..6915
                     /locus_tag="ACHA34_000663"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8791335.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            6954..7100
                     /locus_tag="ACHA34_000664"
     CDS             6954..7100
                     /locus_tag="ACHA34_000664"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791336.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            7538..8878
                     /locus_tag="ACHA34_000665"
                     /pseudo
     CDS             7538..8878
                     /locus_tag="ACHA34_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9119..10735)
                     /locus_tag="ACHA34_000666"
     CDS             complement(9119..10735)
                     /locus_tag="ACHA34_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8791337.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            10853..11071
                     /locus_tag="ACHA34_000667"
     CDS             10853..11071
                     /locus_tag="ACHA34_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8791338.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            11266..11727
                     /locus_tag="ACHA34_000668"
     CDS             11266..11727
                     /locus_tag="ACHA34_000668"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8791339.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(11920..12159)
                     /locus_tag="ACHA34_000669"
     CDS             complement(11920..12159)
                     /locus_tag="ACHA34_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8791340.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(12183..13706)
                     /locus_tag="ACHA34_000670"
     CDS             complement(12183..13706)
                     /locus_tag="ACHA34_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM8791341.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(13724..13846)
                     /locus_tag="ACHA34_000671"
     CDS             complement(13724..13846)
                     /locus_tag="ACHA34_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8791342.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(13876..14859)
                     /locus_tag="ACHA34_000672"
     CDS             complement(13876..14859)
                     /locus_tag="ACHA34_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8791343.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(14883..15032)
                     /gene="rpmG"
                     /locus_tag="ACHA34_000673"
     CDS             complement(14883..15032)
                     /gene="rpmG"
                     /locus_tag="ACHA34_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8791344.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(15053..15430)
                     /locus_tag="ACHA34_000674"
     CDS             complement(15053..15430)
                     /locus_tag="ACHA34_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791345.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(15462..15641)
                     /gene="rpmF"
                     /locus_tag="ACHA34_000675"
     CDS             complement(15462..15641)
                     /gene="rpmF"
                     /locus_tag="ACHA34_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8791346.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(15656..15925)
                     /gene="rpsN"
                     /locus_tag="ACHA34_000676"
     CDS             complement(15656..15925)
                     /gene="rpsN"
                     /locus_tag="ACHA34_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8791347.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(16448..18214)
                     /locus_tag="ACHA34_000677"
     CDS             complement(16448..18214)
                     /locus_tag="ACHA34_000677"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8791348.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(18174..19927)
                     /locus_tag="ACHA34_000678"
                     /pseudo
     CDS             complement(18174..19927)
                     /locus_tag="ACHA34_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(20037..20606)
                     /locus_tag="ACHA34_000679"
     CDS             complement(20037..20606)
                     /locus_tag="ACHA34_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFM8791349.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(20624..22039)
                     /locus_tag="ACHA34_000680"
     CDS             complement(20624..22039)
                     /locus_tag="ACHA34_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8791350.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(22050..22718)
                     /locus_tag="ACHA34_000681"
     CDS             complement(22050..22718)
                     /locus_tag="ACHA34_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8791351.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(22848..23432)
                     /locus_tag="ACHA34_000682"
     CDS             complement(22848..23432)
                     /locus_tag="ACHA34_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8791352.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(23763..23996)
                     /locus_tag="ACHA34_000683"
     CDS             complement(23763..23996)
                     /locus_tag="ACHA34_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8791353.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(24011..24961)
                     /locus_tag="ACHA34_000684"
     CDS             complement(24011..24961)
                     /locus_tag="ACHA34_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFM8791354.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(24958..25800)
                     /locus_tag="ACHA34_000685"
     CDS             complement(24958..25800)
                     /locus_tag="ACHA34_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFM8791355.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(25802..26521)
                     /locus_tag="ACHA34_000686"
     CDS             complement(25802..26521)
                     /locus_tag="ACHA34_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8791356.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            27069..27371
                     /locus_tag="ACHA34_000687"
     CDS             27069..27371
                     /locus_tag="ACHA34_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8791357.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            27443..28669
                     /locus_tag="ACHA34_000688"
     CDS             27443..28669
                     /locus_tag="ACHA34_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8791358.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(28757..29149)
                     /gene="rpsI"
                     /locus_tag="ACHA34_000689"
     CDS             complement(28757..29149)
                     /gene="rpsI"
                     /locus_tag="ACHA34_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8791359.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(29165..29593)
                     /gene="rplM"
                     /locus_tag="ACHA34_000690"
     CDS             complement(29165..29593)
                     /gene="rplM"
                     /locus_tag="ACHA34_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFM8791360.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(30127..30594)
                     /locus_tag="ACHA34_000691"
     CDS             complement(30127..30594)
                     /locus_tag="ACHA34_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFM8791361.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(30714..32252)
                     /locus_tag="ACHA34_000692"
     CDS             complement(30714..32252)
                     /locus_tag="ACHA34_000692"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFM8791362.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            32332..33018
                     /locus_tag="ACHA34_000693"
     CDS             32332..33018
                     /locus_tag="ACHA34_000693"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFM8791363.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(33219..33746)
                     /gene="msrA"
                     /locus_tag="ACHA34_000694"
     CDS             complement(33219..33746)
                     /gene="msrA"
                     /locus_tag="ACHA34_000694"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8791364.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(33830..34507)
                     /gene="pgmB"
                     /locus_tag="ACHA34_000695"
     CDS             complement(33830..34507)
                     /gene="pgmB"
                     /locus_tag="ACHA34_000695"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8791365.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(34504..35634)
                     /locus_tag="ACHA34_000696"
     CDS             complement(34504..35634)
                     /locus_tag="ACHA34_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8791366.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(35634..37817)
                     /locus_tag="ACHA34_000697"
     CDS             complement(35634..37817)
                     /locus_tag="ACHA34_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFM8791367.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(37814..38839)
                     /locus_tag="ACHA34_000698"
     CDS             complement(37814..38839)
                     /locus_tag="ACHA34_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8791368.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(38884..39711)
                     /locus_tag="ACHA34_000699"
     CDS             complement(38884..39711)
                     /locus_tag="ACHA34_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8791369.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(39708..40775)
                     /locus_tag="ACHA34_000700"
     CDS             complement(39708..40775)
                     /locus_tag="ACHA34_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFM8791370.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(40801..41637)
                     /locus_tag="ACHA34_000701"
     CDS             complement(40801..41637)
                     /locus_tag="ACHA34_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8791371.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(41634..42503)
                     /locus_tag="ACHA34_000702"
     CDS             complement(41634..42503)
                     /locus_tag="ACHA34_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8791372.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(42573..43856)
                     /locus_tag="ACHA34_000703"
     CDS             complement(42573..43856)
                     /locus_tag="ACHA34_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8791373.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(43870..45555)
                     /locus_tag="ACHA34_000704"
     CDS             complement(43870..45555)
                     /locus_tag="ACHA34_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8791374.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            45830..46855
                     /locus_tag="ACHA34_000705"
     CDS             45830..46855
                     /locus_tag="ACHA34_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8791375.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(47450..51103)
                     /gene="rpoC"
                     /locus_tag="ACHA34_000706"
     CDS             complement(47450..51103)
                     /gene="rpoC"
                     /locus_tag="ACHA34_000706"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFM8791376.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(51182..54799)
                     /gene="rpoB"
                     /locus_tag="ACHA34_000707"
     CDS             complement(51182..54799)
                     /gene="rpoB"
                     /locus_tag="ACHA34_000707"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8791377.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            55527..56495
                     /locus_tag="ACHA34_000708"
     CDS             55527..56495
                     /locus_tag="ACHA34_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8791378.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            56513..57418
                     /locus_tag="ACHA34_000709"
     CDS             56513..57418
                     /locus_tag="ACHA34_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8791379.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            57415..58221
                     /locus_tag="ACHA34_000710"
     CDS             57415..58221
                     /locus_tag="ACHA34_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8791380.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            58378..59346
                     /locus_tag="ACHA34_000711"
     CDS             58378..59346
                     /locus_tag="ACHA34_000711"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFM8791381.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(59663..60187)
                     /locus_tag="ACHA34_000712"
     CDS             complement(59663..60187)
                     /locus_tag="ACHA34_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFM8791382.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            60474..>61538
                     /gene="scm"
                     /locus_tag="ACHA34_000713"
     CDS             60474..>61538
                     /gene="scm"
                     /locus_tag="ACHA34_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM8791383.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        18671 a        12347 c        10305 g        20215 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2521 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2581 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2641 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2701 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2761 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2821 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2881 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     2941 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3001 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3061 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3121 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3181 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3241 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3301 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3361 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3421 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3481 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3541 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3601 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3661 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3721 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3781 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3841 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3901 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     3961 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4021 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4081 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4141 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4201 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4261 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4321 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca
     4381 tgtatagaag aaacatctca ttaaaatcac cagtaaagaa atggaaatct ttagtttttt
     4441 taatattatc agttagtaac tttttataat cataaaaaaa ataagtagtt ctatctgagc
     4501 gatactcttt tataatactg aattcggaat taaaaaccac gataggtaac tttaaacaaa
     4561 catgaagaga ctttaataag acttctttat ctgaaaacac ttttgtttct ctctttcctt
     4621 tctacatcat taaatagtat tttatacgtt atctaaaata ctattcctct gtcaaaataa
     4681 gctaacttac ttcattataa aatatccact tatcgctttc atgtatattc caccgtaaaa
     4741 agtacatttt atcaccgaga actcatcatt tttcatcatg atgaatacta tttacatata
     4801 tctagttcaa agacaataat tattatgatc cgttattatc taagcactat acagataata
     4861 ggaatttttc gtttctaaat attccaattt gggaatccaa taaaaatttt cgttcaaata
     4921 cgtgtctatt cgaactcttt tctagttgtt aattatgaag ggggaagaaa taaaaaatac
     4981 aatctataca cgaataagaa tattatccaa aaggaggttt tagaaaagat ttattatacg
     5041 taaaaagctc aagacatttt cttagccata aggacatgtt tgatatttaa gaagatatat
     5101 gaattttctt ttctaagtat aaagataact atccattatc aactaaaaaa acatttttga
     5161 aaataaacat cagcagtcct atcaatgcat gtgagcttat agaagttatt cgcagcgcag
     5221 cataattgca ttagcttctt ttcgtgtttt ttgcctcttt tccctttaaa tcttaatgga
     5281 ggaggtgtgc aatatgtcag ataacgacga acgcgtaaat gcccagttga ttcgattctt
     5341 tgaaaaggtc attttgaaca ccgctaatac ttattataaa aaacaagaag aaattcaaaa
     5401 acatgaacaa cttgatcaac tcccaccata tttgtttact aaagatacta taaaatttaa
     5461 acaaccgatt aatgttttaa atgtgactct ccttgttgaa gatataaaat tagcaaaaat
     5521 ccttcctttg ttgaaagaac ctgaacaaat ttttcttatc gaaaaattta tctttgataa
     5581 aactgataaa gaaattggaa aggttctggg gattacaaga cagggtgcga caaatttaaa
     5641 acatcgactt tacaaaaaaa ttcaaaaaat gctggtttaa tatctgcata ataactaatt
     5701 ttgtatgctt ctttgctcgt tctcctcctt cccaaagaaa tagattataa gtccatggga
     5761 agaaggtaga tttatgaatt ctttaaaaga agatttcatt ttagcaaagg ctggaaatga
     5821 agaagctgtt gaggcaattc tcaaaagatt tagctctttg attcataagc aatcctggag
     5881 aactggaaaa tatgatcaag attgttatca agaatgtatg cttgcaatat atctagctat
     5941 ctcgaaattc gaaattaaag aataactcaa ctaagggagt ctgtgacata agtaaaaaga
     6001 tagtttcaaa tttcgaacaa taaatttgaa actatcttct ttataattat aaagtatcga
     6061 ttttacagcg ttctataatg attcatagaa tctaaaacga atgattttaa ttatcaacgt
     6121 tttttaattc gtttttagct taaaaataac ttttgtcaca gtctcactcc taaaattact
     6181 ttatttacgt atgaagtggg caggaggtcg aatccagaac tgggctatgg tacttcaacg
     6241 aggacttctc ctctagaatt gaaaagcatg cgatttacct tccgtaacga cggtagattt
     6301 attcaactat gtgtccaatc aaaatttgtc tgatttcaaa aatagtcaag agaattttgg
     6361 ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt gacaaaccct gatactaagt
     6421 tgggctatga tacacttttc ttgactatca gttacgttac cggaaggaat gaactctatt
     6481 gagaaaaaca tatgatcgta aatttaaact aaagatttca caggatatcc ttgagaaaaa
     6541 agtaactacc aagaaaattg ctgaagaata caatatctct cgtccaacaa tttcccgatg
     6601 ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct ggacaaggaa aaagattacc
     6661 tgataaagca gatttctaca ttcttgaaca ggaaaataaa cgactaaaag aggaagacga
     6721 gtgttctacg aacacaaaga tcgatacgga tctccaggga tcactcaaaa actttacgat
     6781 gaaggaatcg aaatcaataa acgtgttgta gctgttctta tgcgtgaaat gaatttatgc
     6841 acaagaggat ttcatcgacg caaatcttct catggtagag aaaaaacaat caaagaaatg
     6901 gtaaaaaaat gttgaatcga taatttgaac agaaccaaat tgatgctgtt tgggtgatgg
     6961 atattactta cattccatgt aatgacggtc gcctctattt gtccacctat attgacttag
     7021 caactcgtat tctacgttac tatatggtag attcacacat gaaaaaagag attgtcgtcc
     7081 gtccattaca aatttattaa ggagaattgc ctacggtaat tcattccgat agaggtagcc
     7141 agcatcggtc ttttatttat caagagctgt tgaacgacca ccacatcaca cattcagtga
     7201 gccaaactgg gactcctgtt gacaatgcag taattgaagc gtttcatcga tcaatcaaat
     7261 gagaattaat cgactccaac aaatataaaa gtaaaataga gatgaaggtg ttaatccaag
     7321 attatttaga agactactac ccaaacaaaa gaatccatac aaaatttatg atgactcctt
     7381 gacaatttga agcgaagaaa ataagggatc tataatatct agtgttggtg acgccttctg
     7441 ttgccaatac tttttctttt ttctaataaa cgcaaacaga caccttaaaa tcctttagaa
     7501 taaagtcgac gaaaaccatt caaaggagtg tttcaagatg tcctattcag agtttaccaa
     7561 agagttacta gatattctag acctaaatct tatctttcat gaagatgcct ttcgcaaaga
     7621 gcgaatcaat gacgaaacat gttttgtttt tgatggcaca ttgacctatc aaccggagga
     7681 atgctttcat cgccactatc aaaatgaaca aacgatcatt aaatagggct ggaaaaaagt
     7741 atccatctta ttgaatgatg tcagtaacta caagaccatt ctgcggatta acaaacagcg
     7801 cttcaaatgt aaacactgtg gaaagacttt tttagcagaa gattcagtta gtgatcgtcg
     7861 ctgctccatt gctcgcagag tcaaacaagc cattcttgag ctattaagcg aacctctttc
     7921 tatgtctttg atcgcacgta tgaagcacat ttctccgacc actgtcattc gaatacttcg
     7981 cagccttcgg cctaaaacca tttctttgag ccagctgtta ccagaagtgg tgtgtttcga
     8041 tgaattcaaa tcagtgaaaa atgtctcggg agccatgagc tttgtcatga tggacggcaa
     8101 gacccatcaa ctgattgata ttgtggaaaa tcggcaactc aatcctttac gcgactattt
     8161 cttgcgctac cctataaaag tccgtgaaca agtgcgtcta gtggtttcag atttctatac
     8221 accctatcgc acattggtga aagagtgttt tccgaatgcc cgaatcgtcg ccgatcgctt
     8281 tcatattagc caacacattg gtcgggcttt taccaaccac cgaattcaag taatgaagac
     8341 ctttaaaaaa ggggatcgtc ggtcgaagca cttaaagaaa tactggaaac tacttcaaaa
     8401 gaatgcgtgg gaattgaaag gtcagcatcg ctactggcgt ccgtcatttc gagatcatct
     8461 gacagaagca gagatagtcg atcggctatt atcttatgat gattccttaa agcgaggcta
     8521 cgaagtctac caggattttc tttccgttat cagaagacaa gatgttcctg aattcgtcgc
     8581 gctcctaaaa gaagactaca aggagttgcc agagcactac caaccagtgt tcacgacctt
     8641 caagaaatac caaacagaaa tcaaacgagc gttgcgcgtt ccctattcca acggccctat
     8701 agaatgtttg aataaccata tcaaagtact aaaacgaatt gcctatggct atagaaactt
     8761 tcaaaactac cgagaacgaa tctttttata tcgaggaaaa tattttaaga aaactaagaa
     8821 tactacccaa ctgactaaag ccagaacaac aacacggcta gacaagatag ctgtctagcc
     8881 gtgtgagtat ctcgattatt ctattgaatt tcttcaccaa cactcattga cgaagagcca
     8941 aaataagtaa tctaaccgac tgttaactaa accattgcag gtcccgttaa agatacttgt
     9001 gcctgttgtc accgcttctt tttcttgtcc acaagcgctt aacacactta ataaagcccc
     9061 tgctattatt aataataata tctttttctt attaaaaacc tcctcatcta tttccagatt
     9121 agtaaatgta gtaatctgtg ctttcttttt tctcaatact aataactaac ttatggggta
     9181 aacaaatact cgtttgaccg ggttttgaga tccatccagt tctaaccgcg atttgatcag
     9241 gactattatc ttctttaact cgaattcttc catctttaat ctcaacaata ttatattgtc
     9301 ccttcgcagg atgataattc acgagtttat gattgatctt atccaaatcg aagcgatcca
     9361 tttccttgcc atcaattcga acgatggcat atttgatagc atcttcagtt gtttcattct
     9421 ccttttctat agagaataca aataagggaa taaaagaggt aataatcaaa atgccaataa
     9481 ttatgacatc gcctatcttg acaccagctt ttcttaaaaa tttcattaac gatatctggc
     9541 taaacatatt agactttttc catggaaatg gtgaaatttt tcggaaatta tctgcattaa
     9601 gagaatcagt taaacaaccc tcaagttgtt ccttaataca ttgttgatcc atcttcatac
     9661 caataaaaac tagtttatta atgcgttcac caaacaacgg atgccaggct tccttcaatt
     9721 cgggattttt ttgtataatc atttgacact caattttagg taaactagct acccaataat
     9781 ttaatgattc aatactttta tagctacccg atgtttcaaa gatcgtcgaa aaatcactat
     9841 gttgggcaaa ccaaagaaac ccttttgctc ttgtaatatt gccaggaaaa tttttatccg
     9901 taagatgggc aaatttttgt gcgtcaagga gtaaccgatt tcgatagata aaacttgaga
     9961 taccatattc ttctgtttca ggaatatgat tttgatagcc aagctctaac tctttgaacc
    10021 agcccactga ataggaagtc tcatctatat taaaaagatt tgtatttaga attttcgata
    10081 agtctacttc acctttaatt gtcctaatta ttttggcatt ggactgtaac ttagcaatta
    10141 gaccgttaat tttgtaaatc gtcttggttg acactaagtc actattattt aaaagtaata
    10201 tgttacaaca ctcgaattga tcaattaaca agccacgaat atcctgttct tcttcaacta
    10261 cattaccttc agcttttctt tcctgaatct tttcacccgt ttcgaaatta tcccaaaaac
    10321 gttttacatc cactactata ataatggcac caatctctaa tcgttgggtc aaatcaatgt
    10381 ctaaagggtt tttagctaat ataattgctt gaataattgg aattggtttg ctgattcctg
    10441 aagcttcaat aacaatttga ttcaagtcag aaaattgcac taattgatct agttcaacaa
    10501 tcaaatcttc gcgtaatgta caacaaatgc atccattcgg catcgaaaca agtttttctc
    10561 cagttctttt aaaacctttc ttgtcgacta ataccccatc aacatttact tcccctatgt
    10621 catttacaat gacagcaatt tcctttccgt gatcaccgta cagaatatga tttagaagta
    10681 tcgtttttcc tgaaccaaga tagccagtta agattgtaat aggtattttt ttcataggga
    10741 acctctttct ataccttgaa tttacacttt aagtgcaaca cataaaaatg agtcataatc
    10801 gatatccaac tagtataagt ttgtcgagaa cactcctaga aggaaaataa ttatgagcca
    10861 gtatcgtcat cttactatac tcgaacgaga aagaatcttt ctgcttcacg aagaaggatt
    10921 ttctattagt ataattggca acgacattca gcatagcccg tttactattt tacatgaact
    10981 gagaagaaac aagaaaaaaa ctattcacct tccaaagcgg aaaaaaatta tgcttatcga
    11041 agaaaaaaat gcggacgtta tcacattcta aatgatccgt gagtttggtc aattgttcgt
    11101 catttatttg tcgattttca gtggtcgcca gaggaaatct cgaatcgttt gaagcatgaa
    11161 catatggact tttcaattag ttttatcacg atctaacgtg caatttgcaa tgatcttttc
    11221 gatacagaac ccctctcaca cggcaataaa ggcctgatcc gttcattgca ccatcgagga
    11281 aaaacaagac atacaaaaaa tcatgtcgag tctcgtggaa agatttgcct tactcattcg
    11341 atccatggcc gtccaaaatc cgcagatgat agaagtgatt ttggtcattg gaaagccgat
    11401 attgtagctg gcaaaactgg tggtacttgt ttagtgactt tgacagacag aaaatcatgc
    11461 ttccttttag caggaaagaa ggtccccaga aagaaagccg cttatgtgag cgagaaaatg
    11521 attgaacttt taagcagtat tccaaatgag gcggtcaaaa cgatcactcc agatagagga
    11581 aaagaattct ccaatcattt cgcagttaca gaagcattga ataatatgcc cttttatttt
    11641 ccagatcctc atgcccttgg caacaatgaa caaattaaaa tacgaataga ctcattcgag
    11701 aatacttgcc aaaatcgaaa gagatagcta cggtagataa ctcaacgata aatcagatga
    11761 ttgaaaaact gaataacaga cctagaaagt gtttagattg gagaacacct tatgaagtct
    11821 tctttgataa agtgttgcgc ttgatttgac aattcaagac ataataaaaa gcagatcaaa
    11881 tccaaataag ggaaaaatga tctactctgg tattatcaat taattggcca agctattatg
    11941 ccgttttgct aaacgtctac ttcttccagt attgaccttg ataaaacaac aatcaggatc
    12001 ccgttttaat actctatgaa ctgtgtcaat actaacatgt aaagttgcag caatatcagc
    12061 taatgtactc ccatttaatc tcatttgctt tgcaaatcga taacgccgat aagcatcaat
    12121 attcttggaa tccatagaca ataatacttt tcttaccatg tatttcctcc aaaaattgaa
    12181 ttttaatgag ccatcatttc ttgagcaatt tcatgtttag acaatgaagc tggatagaaa
    12241 gtaggccaat tatgcaattc attatagagt ttttcttgac tttctccacc aaagaaaata
    12301 tggaaatgct ctgccttact tggtgcaatt ccatgatcac taaattgaat atatttaaaa
    12361 cgaccagcac ctgcatcatc agtttcaaaa ttaaaacgaa caccacggtt tcctttttca
    12421 taattcaaaa ttttgtatcc aacatatttg tatgtgtatt gatgatgttc accgttgact
    12481 acaaaatcaa tggtgttgtc tttaatgttg atagtatcaa tatctgtctt atagccagta
    12541 gtataataat ctttgtactc ggcagctgtc atgtctttat tgatttttgc tttgtaatcg
    12601 aaaacttcat ccaaaattcc atcttttaac aacggataaa cagattgcca ttcgcctgta
    12661 taatcactca attcgcgatc cttcacatcg gcatctttaa agtacccatt agcaactgtc
    12721 ttttcatcct tcgcttcttt ttctggaact acctcttcac cagcaaccat agttgttttt
    12781 tctaacgctt ttagattgtc ttccattact gaaacataat cttcaccatt atccatttgt
    12841 tcttttgtaa gactttcaag aggattcagt acttccaatt ttatcccagc ttcgttggcc
    12901 aaagtttttg caattttatc attagcattc ttttcaaagt aaatataatt gatcttattt
    12961 tttttaacat actcttttaa ttcagctaat cgaccagaag atggctcttc ttcaggactt
    13021 agtccagcaa taggaacttg tttcaatcca taatccaaag ctaaatatcc aaaggctgcg
    13081 tgttgcgtca caaaactttt ttgtttcgct tctttaagac tagtggtata gtcagcatcc
    13141 aaccgtttca gtttagtcaa atacttttca gcatttgtct caaatacttt tgcttttttc
    13201 ggatacaatt taactaactg atccttgata tttgaaactt ccttaattgc cattttgggt
    13261 gaaacccacg tatgaggatc tagttcatga tggtgatctt cttctccgtg atcatgatct
    13321 tcttcttcac tacctggcaa taaaatcatg tcttttgttc cttctactac attaggccca
    13381 gctttcttcc aactttcttt agcttttggt acccatgatt ccatgttttc gttatgatat
    13441 acaaaaacat cagcatcatg agcttttgcc atatccttag ctgagggctc atagtcatga
    13501 ggttctgatc ccgctggaac cattaaatcc acgttcgctt catcgccgac gatgtttttg
    13561 gtgaaatcat acattggata gaatgttgtt acaatttcta attttccatc tttatcattc
    13621 gatacttttg catcattagt gctggagcaa gctcccaata atcctagagc tgctatccca
    13681 ataagtccta cactgatttt cttcattctt tttccttcct taattatgct aatttcttca
    13741 ttttatttcg tttatgttct ttgataattt ttaatgccct ttttctagtt ttaatattcg
    13801 ggcttttaag tcgtcgatat gcactagata tatccgtttt ctccattaga aattaccccc
    13861 aatcactact tatcattatt tgctttccgc cagtaattta tcaaaactct gtttgatcct
    13921 ttcttcatcc aacatttttc cgattagaat aatagtggtt tggtcttttt gtcgagtggt
    13981 gtattgaaaa ttaacctgtt cgtttacctc ttgcatggca actataaaat cctgctcttg
    14041 taaagcaatc aatcctttaa aacgatacag gctcccttgg ttggttaaaa tcagccagtc
    14101 aatccattgg agtaaaaaat ctttttttat gtcttgatct gaagttaaga gaattgattt
    14161 aaactcatgt acttgatggt gttgatgata gtatttgcca ttatagtgat tttctgatga
    14221 atcactttca aaaactaagg gatgattaaa ttttttcaaa ttataaaaat cctttttttg
    14281 aatcgtttca ctatgtgtga acattcgaat atcggataaa gggttaatac ttttgatttc
    14341 tctctgtaat ttttttagtg cagataaatt caagttttgt tttctagaca caaaaatgcg
    14401 atctgccatg acaagttgct taagggcttg tggttctctt tcaataagtt caaaactgtc
    14461 actatcaata acagtcaata aactatcgat atatactctt cctcttagtt gaggcgctgt
    14521 taaaagtgtt tgaatgattg gttggggatc tgctatacct gtagtttcaa tgatgatttg
    14581 tgaaattggt tgttgatgtt cttctattgt ttccagtaaa gaaatcaata ttgctaataa
    14641 atcactgcga agactgcaac acatacatcc attatttagt tgtaacaccc gttcctcgct
    14701 atgaactaat aatttgtgat caatcccaac ttcaccaaac tcattttcaa ttataataat
    14761 ttcttccctg gggtagggtg agacttgcaa ggcttgattg attagcgttg tttttccagc
    14821 ccctaaaaac ccagaaacga ttgtaactgg tatgcccatt ttgtcacctt tttaccactt
    14881 agctatttca cttctttaaa aattgcccgt ctgcgcaatt ttggcgaata cttttttaat
    14941 tctaaccgtt cagggttatt ccgtttattt ttgctggtga gatacagacg ttctccagtt
    15001 tcgacacatt ccaaaataat attttgacgc ataaatcttc ctccttcaat acttatcgat
    15061 cttcaagata tgcttgaatt tgttgtgcat cttcaaaagt taactcgcca gattccaatt
    15121 gtgaaactaa attttctttc atatcaacag gtatctccca ataaccatcg atacattgaa
    15181 ataactcctc gtcatactct tcaaaaattg gatagattct taaaaaatca tcaaatgata
    15241 agccttggtc ctcatagtaa tctttgacta atccattttc aattgcctgt tgggtcggta
    15301 aacccgtttc agtaataatt cctctctcta ccatcgaacg ataaaaagtg catttatcta
    15361 gcgattgctg ggtcgtttca agataatact ttaatccttc ttcaactaaa ggtgggtaaa
    15421 taatttccat tttgattccc tcctaaaagt tatatattaa actattttga agctccttta
    15481 attactttct ttccattata gtaccctttt aaagatacat gatggctacg tctataatca
    15541 ccattcgctt cgtcaaatga aatttctggt tttactacag tttgatgagt tcttcttaac
    15601 cgtttcttcg ccttagatgt ttttcttgct ggtactgcca tgatatttcc tccttttacc
    15661 aacttgcttt ttttactcca ggaatcaacc cttgatgggc aagttctcta aacttaatac
    15721 gggacatacc gaactttcgc atataccctc ttggacgacc atccgtctga tcacgaagcc
    15781 tcagacgatt cggatttgaa tctcttggca atttagcgag agcggtgcga tctcctgcgg
    15841 cttttaattc ctgacgttta tctgcatact tttcaatcat cgccatttgt ttctttgctt
    15901 tagcaatttt cgactttttt gccatatacc tttatactcc ttttattttg atttagacac
    15961 ctcaaatcgt aatcgttacg ttttgacact gatactttac caacaagagt tgctcttgtc
    16021 aacaataaat aacatgaaat aaaactcata tgaatagata actatttgaa aacgaattta
    16081 tcggtacata gagataatca aatcatttaa aagaatatgc agtttaggct ctgaaaaata
    16141 agaattcact tttattagtc caatcaaaca caaataccgt tttaagtagc ttttcctcta
    16201 cttaaaacgg caatatgaaa gaaactataa ctttttattc aatcttttat gtttaactgg
    16261 tttatcgcca attttttaaa actttgatta ttccttatat tataagaata gacattaagt
    16321 gaactaattc aatcatactg attctttcgt gtattcaaaa ttagatgctg tttaataaaa
    16381 ttattctttt tgaaaaagaa ttataactat atcccaaaaa ttgtaggtta atttaaagcc
    16441 tatttcatca tcgtgattga gcttcttttt catagacgaa tttctcagat agtttagttt
    16501 tctttatgag atttttgtat atttctgacc ttcgatacaa ctcattatga gtaccttgat
    16561 caactagttt tccttgatcc atcactacaa ttttatttac cttttcaata gacttcaagc
    16621 gatgcgatat aattagtact gtcttatctt gtattaaacg atttaaactt tcctgaatac
    16681 gtttttcatt ttccgcatct aaactggcag taatttcatc taaaattaaa attggcgcat
    16741 ttttcaaaaa agcccgtgct atggataggc gttgtcgctc tcctccagaa agtgaacttc
    16801 cgttttctcc gaccaatgta tgataaccgt caggtagttt ttcaataaag tcttcgcagt
    16861 tagctaaacg agctgctttt ttcacttcct catctgacgc ttgactattt ccaatacgta
    16921 tattttccaa aatacttgta ttaaacaaag ttacctcttg aaaaacaata gcgattttgc
    16981 taaacaaaga agctggagat acacgtttaa tatcataccc atcaattaaa atacagcctt
    17041 catcataatc atacagtcta gacactaatt tcaagatact agttttcccg gaaccactag
    17101 cacctactaa cgcagtcact tctccttgtt tagccgtaaa agagatatga tctaaaattg
    17161 gggtattgtt atcatatgag aaagagacat ctcgcaactc aatgtcaaaa gatttcagag
    17221 gactatctga tccctcttgg atactggttt ccttcatagc tctaattcgc tgtattttag
    17281 gagctaaata aaatatttca agcactgctt ctttcataga atcgaaggaa tcctttattt
    17341 ttattgcggc tagtaagtaa ccaacgacgt agagtatagt cacttcaccc gttaacagta
    17401 agtgaacacc tactagaatg actactgcta aactaacata tgagaaaata gaggataagg
    17461 ccatgactga aaaagtactt aattctacct ttaaatgaat cctttcactc tcttccattt
    17521 ttttgaaaag tctgtcttga acttttttag atagattaaa gctattaatc tcttgctgca
    17581 attctatagt ttcttgaaat tcttccgagt tttctctcaa agtatcgtaa tatttagtat
    17641 tggctttcgt ttgggatttc tttgataaca agattaagac aaaactaaat aacgttggca
    17701 aaataacagc taatcccatt ttgacattac caaccaaaag catcactgaa ataaaaggga
    17761 aaaacagagc cataccgccg gctttaggta ttgcatgact catcgcatgc tcaatacctt
    17821 caacgtcaga catgatagtt tgagataaat ctgataaatt atgttttgaa aaataggata
    17881 atggtaaatt tgacaaatcg tctgcaattt gcactcttaa atgcgcactt tctttataag
    17941 tactattata taagttttca tactccctat ctaacaacca atacatgaaa agcaaagtaa
    18001 caaacgatac ggcaaaataa agccaacggg gctttgtatt ttctaaaact aattcgtcta
    18061 tcagcatcat tataatgaat gctggtacca tattaatgca gtatacaaaa aaactagaaa
    18121 tagaagcttt agttaaagct tctgcgccct gatcacttaa agcaaatttg tttttataaa
    18181 aacttttcat tgtcaagcct ccaatcattt gcattctcat aattagtgac caattgttta
    18241 tacacttgat gtttagccat taattcatta tgatttccac gttctactat tttcccttct
    18301 gacaagacaa gaatttcatc tacagattta atgctggata atcgatgagc tatcataata
    18361 actgtttttt ctttcatcag atgttgaaaa gccttttgta gttcatactc attatctgga
    18421 tcaatagatg cactggcttc atccataata acaatctttg catcttttaa aattgctctg
    18481 gcaatagcta tacgttgttt ttctcctcct gacagataaa ctcctttact accaattaag
    18541 gtatgctctc tttcgggaaa tttttctaaa atcgattcgc aaccggctaa tcttagcgcg
    18601 tcaaatactt tacttgctgg cgcttgagga tttgctatag aaacgttatc gtaaatactc
    18661 tgattgaata gtttcgtatc ttgaaagaca aaagcaattg tgttaataat tgtgtctttt
    18721 gaatagtgat ttaaaggctt accaccaatt ttaatctgtc ccttatccac attgtagaaa
    18781 cctgaaatta gtttagctat cgtactcttt ccgcttcctg attttcctac taatgcataa
    18841 attctttttt gttctaaaac taagttgaaa ttttctaaaa cacactcttc tttgtatgaa
    18901 aagctgacat tttcaaatgc aatgtcacaa tttttaatct gtgtctcgtt cccatatttt
    18961 agttggtctt gcttcatttc taaataaagg gagtctagtg tttcgactgc atatcttgct
    19021 tcaaacaagt gcatacttac atacattatt ctcatgaaag aaacaaaaat aattccactt
    19081 aaaaaaaaca acatcaacaa gtcaacagct aatatcttgg gagtggtaag agaagttata
    19141 aaaaaggtta taggaactat caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga
    19201 acaaaaggtg ttttcccact caaagaatac ttatatgcaa acttagcata atcgttaatt
    19261 gctttattca aggctttaaa tgatgttact ttagcaccaa atatctttat aacttgaatt
    19321 ccgcggatat attctaccgt ttcagagctt aacacatcta acgattcttg atatttttga
    19381 ataaaatttg tattctctgt cattttcttt aacaagaata caccaattac taacatggcg
    19441 attagaacca atccaatttt aaagttaatg aaaaagccta aggcaactga taaaacaggt
    19501 gttactatgg cttgagcact atctggaatc aagtgagcaa ttgccatatg tgttttagcc
    19561 gcgttgtcat caattgtctt tcgaatatat cccgaagaat ttaaatcaaa aaaacgaaaa
    19621 cttgcttctg ttaaaccgct aattcccttt tttctcaaat tagtctctaa gcgaaaccca
    19681 aggatatgag aaaataagcc agaaaaaata taaaataaag ctctaaagtt aaccaagcaa
    19741 cagtttgaac cgcataactc ttagctaaat attcatctcc tacaataatt agagactgaa
    19801 gaaatttgta aatactgtaa tatccataga ctgttaatac gacagaaaaa tcagaaaata
    19861 aagtggctaa ccatccgtag tatttcattt tagggacata actaaataat tttttgtata
    19921 ctttcatttt ttcctccccc gcattcaatt tcttctctta ttcgtttaca actgttaatg
    19981 ctttccgtaa tagcgtaaat tagagatgta caaacagata aaaggtaaac tacgttctat
    20041 tttttgtatt tttttaagac ttttcttcca atcaatacac caatcataga taaacttgct
    20101 acaactaaca ctgaaatgat tccccatata cctaccgcat agtggatcat tgcatccatt
    20161 gttgcagcag attgtccttt atctatccaa tcttgacgaa aacgatctgt aaagaaccat
    20221 ataggaatcc actgtccggc agcactgcct aattgcatta gagcccaaga cgcaacttga
    20281 ggaattgctc gatcatatcc aaatacagat agaataatat cagctaagat cgccatacta
    20341 atcgaactga taaaccaagg taagtacgcc ccgcccatta acgtaaaaaa taacaataaa
    20401 acaattgtgt aaatagtaaa aattcctttt ctagggtagt tatagctaat ataaacaaat
    20461 atgattccag ataataatcc aaaaatcccg ggggatatgc tgtgaccaaa cgccccacca
    20521 atactactaa ccattattga aaaaaagtaa attactaggg aaataactgt agctaaacca
    20581 ataaaaatat aatcttttgt tttcatatat attctcctca attctatttc gttatgtcat
    20641 taaaaatctg ttctatcaaa gttgaatctt ctactctaag ttttatatct ttttttattc
    20701 caccattttt taaataaata attctattgc atgttttata aagaaattcc ggatcatgag
    20761 tagctataac tactgcaccc ttttctttta agtctttaat aaggtcagca atcctttcca
    20821 tattaatata atccaaccca cttgtaggtt catcaaatag aaataaatta gtttttgaca
    20881 atgaagctat agaaaccaat aatctttgtt tttctccacc ggataaatca aacggtaatt
    20941 gatctttcat tttataaagt tccatttttt ttaaaatttt ttcttgctga tctttactaa
    21001 ctatttcatt ccccagggct aactcgctcc tcactgattc tgtgaaaaat tggtaatcca
    21061 tttcttgcat tactaaaaac ggcgatgaca aatttttttt attttcgatt atctttcctt
    21121 tggtagggcg aataatcttc atgagtattc ttagtaaggt tgttttcccc acaccgtttt
    21181 tccctaccaa accaataaca tctccaactt tgacatcaaa atttatgtct tttagaatat
    21241 tttcataagt aatttcttca acttttaata catctgcact taccttttcg atatagggaa
    21301 cttcaatcga actattaaaa atgtcaaatt tccttaatgc gttattgttt tcagttttta
    21361 acaactgaat agttgtagtt tttacagttc tatttttaac aaatagaact tgatcaatta
    21421 attctttcaa atagtaaaaa cgatgttcag ctataacaat agtaaatcct ttatttttta
    21481 aatcacaaag taagtcagca agtttcttag tcgctttgaa gtccaaattt gaagacggtt
    21541 catccaatat aattaattta gagtttagcg ataaactaca tccaatagaa attttctgcc
    21601 gttcaccact tgatagcgaa aaaatgtttt tatctaatat atttgaaaga tctaataatt
    21661 cagctaattc attgattctt ttatccatat gctcttttgt aaatccaaag ttttccatcg
    21721 aaaatactaa ctcactcgtt gtatttgttg taaagaattg tcctctagga tcttgactta
    21781 cgtagccaat attttttgat atactttctt gtgttaagga ttgtagcgag ttattttcaa
    21841 ataaaatttc cccaaccaat tttccgtcat aaagtcttgg aataagtcca ttcattaact
    21901 ttaatagtgt ggatttccca ctcccagaag aaccagtaat caatgtaaga ctgccttttt
    21961 ttattgaaaa attaaggtca acaagcgctt gttccttaac atccttatat gaaaaagaaa
    22021 cattgttaaa ttcaaacaat tctttcacct caaatcaaaa tacttaagaa aaaaatagta
    22081 acggaaaaca ggatgatata gtctaataag ctcatgtttg ttttatagta agaacttctt
    22141 ttcacaggag attctatccc ctttgttatt gcattagcgg tcatttcttc agcaatcata
    22201 gttgcacgta taagaacggg aactaacaga aattccaaat attgcagagg ctgaaaaaaa
    22261 gacaagcgaa tgtttctcat ttttaaagat tctttaatca ttcttatttc agaacgataa
    22321 gtcggaaaga atctgatcac tactgtaata cttaacatta tttttttcgg taaatgtatt
    22381 ttttctaaag aggataataa ttcattgcta gtatagacac taaccaacaa ccatgagatc
    22441 attacgaccg gcaacattcg aaatacaata tataaggatg tcgcaaaaaa tatagtaaaa
    22501 aactctatct tgatagcatc aataattttc attactaaaa acagaactga ataaatcaag
    22561 atgaaataaa tacatgtctt tattttacga aacatcaata ctaaaactgt agcaataatg
    22621 aaacacccca cttgaaatat atttcccttt cctaaaaaaa taatgaccgg aaaaaaaata
    22681 tttaaagtaa tcaaggttaa cggattgatt tttgacactc tttcacctcc aaaaaataag
    22741 atcgcactgt tatcttaagc tgataaaaat gcaccgttat tcagataaca gtgcgatctt
    22801 atcaaaaagc ataactgaga acaattctca atgcaagctt tttttaatta actcagtaat
    22861 tcaatccacc caggaacaga aaattgatac aatcttctgg ttaatagcat agtcttttct
    22921 tttgaatagc tatgcttcaa tatatcatag aatgtagcgt agaatgagta aatgtagaag
    22981 tggatttctt cttcattaaa gactaaattt tgattgatca ttccgatagt ctttaactta
    23041 tctagacaac agactgtctt tttcgtcaca aatgatgtaa ttttttcaac aatatcttga
    23101 tatttggttc cagctgattg gaataaaatc aaatcgaata tacgtttatt ctcaaaaata
    23161 taattaataa atggatctaa ttcattgaag ttctctctcc aacttttctc tatatctata
    23221 ttttctaagt ttaagaggta tttggtaaac tcttcattat acatattaat aagcgcatta
    23281 atatgcggct gaataatttc ttcaaataac gcttccttag aagtaaaatg tttataaaaa
    23341 gcacccgtag ttacgcctgc ggtcaggcac agttgcctta tcttagtttg ctgaaatcct
    23401 tctttttcaa aatggcttag tccactttct attaactttt ggtgagttat aatataatct
    23461 ctggtttcta tgggtacttc ctcctaataa gatgaattaa ctttattatc ttacacagga
    23521 cttgaagttt caatcgttaa cgtttattac tgaactcatt ccttagagct aaactaatga
    23581 gaaagagcag aataactgat acaaaaagca atcctacaag aattaataga ctcatctaaa
    23641 tcattcacct tttctaaaat aatccgagaa agatttaaaa aaattatatc cagctattag
    23701 gataagtcct atcataatca ctccaatagc cattttctta tcctccctaa aaataaagtc
    23761 ttttacttaa aacctttttt gtaatcgtca ataaattttt tccctttatc agttaattga
    23821 aatcctatat ctaaatagtc ctcatatcta agtttagcca gcatctcact tacagatgct
    23881 ggactaacac ttagaacttt tgctaaatca ttatttttta atttcttttt tctttttgat
    23941 aattcctgaa ttgctagtaa ataaattagt atagtagtcg ttttaatttg taccatacct
    24001 accctccaaa ttatttcgaa aggccttcag caattttatc caggttccat ttcatcatcg
    24061 catagtaact atcaccatct tgtccttttt ctgcaattga atcagtaaaa atcgttgaat
    24121 agataggaat attggtatct tttgatactg ttttcattgg tctatcatcc acactgcttt
    24181 ctacgaataa ggagggaact tttgttgtgc gtaatttttc aactaagtgt tttatttgat
    24241 ctggtgttcc ttcttcttca gtgttgattt cccaaatgta agcagaaggc acattatacg
    24301 ctttcgagaa atatttaaag catccttcac ttgtaacaat cattttttta tcattcggaa
    24361 ttgaagcaaa tttagattta gcttctttgt ctagagaatc caatttttca atatacttat
    24421 ctagattttc tttatagaat tttttattat caggatcttt ttccgctaac tgtttttcaa
    24481 tatttttagc gtaaataata ccgttttcta aatttaacca agcatgagga tcttccttcc
    24541 ctttctcact ctgaccctct aagtaaataa catctatgcc atcacttgct gcaaaatagt
    24601 ctttgttttc ctctttgttc gcatttttaa ctaattttgt aaaccaagca tttcctccag
    24661 tttccaagtt aacaccgtta taaaaaatca aatctgcttt tgaagttttt tgaacatctt
    24721 caggcaaagg ttcatattcg tggggatctt tcccaatagg tacgatactg tgtaaatcaa
    24781 ttttatcttt tgctatattt tcggtaatat ctgctaaaat cgaattagta actactactt
    24841 ttaatttttc gttcgaagcc actttatctt tgtctttact atttattgaa catgccccga
    24901 atataaaaag agcaatcaaa atccccagac ttcccagtac tgctttccat tttctcatca
    24961 taacgatctc tccttctttc ttaaaaattt ttgttttggt gagattagaa aactaattac
    25021 aaataagaat gccgctgtta aaacaatact tgatcctgct gctatgttaa aactataacc
    25081 aataaacaat ccaagcaatg atgaacttgc tccgactgtt gcagaaataa atatcatagt
    25141 tttcaagcta tttgcatata aatatgctgt agctgctggt gtaattaaca ttgcaacaat
    25201 taatatagtt cccacacttt gcatagctgt tactgctact aaagttaata gaaccatcaa
    25261 tagataatga taaaaactga cattcatccc tattgctttt gccattaatg gatcaaaaga
    25321 agtgattaat aattctttaa aaaatagaac tactataacc aacactaaaa ccgaaacgcc
    25381 aattgtaatc cacttatcaa tatcttgtac agctaaaata tttccaaata aaatatgaaa
    25441 taaatccgtt gagctatttg caactcctat aaggataact cctagtgcta aaaaagaact
    25501 gaaagtaata ccaattgctg tatctccttt tattacacta ttgcttttta taaaagtaat
    25561 aatgatagaa gctaatagac caaatacaat cgcaccaata aaaaaattaa tacctaaaat
    25621 ataagaaatt gctactcctg gcaaaacagc atgagaaata gcatcaccca taagagacat
    25681 tcctcttaaa ataataaaac aacctactgt tccagaaaca atccctataa ctacagatgt
    25741 aattagagca ttctgtaaaa agtgaaagtc atacagacca ttaataaaat tttgaagcat
    25801 attattcccc ctctccaata aatatagtat ctccataggc tttttttaaa ttttcttcat
    25861 taaatacaga ttctacactt ccgtgagcaa ttagatttcg attcaaaaga ataatattat
    25921 caaaatactt ctttaccttg cttaaatcat gatgcacaat tagcactgtt tttccctgat
    25981 gtttcaattt ttttaacgta tccataatta ttcgttcact aactaagtct atccctacaa
    26041 atggttcatc taaaaatatg aaatccgcat tttgagcaag acatcttgct aaaagtacac
    26101 gttgaaattg tcctccagat aattctccta tttgatgatt agaatatttt cccatatcga
    26161 ccttttccaa agcctttgaa actctttgcc attctgcatt tttaattctt tgaaaaactt
    26221 tcattccggc atatgttccc atagatacac attcttttac ttttattgga aaagtaaaat
    26281 caatatcagc tttttgctct acatatgcaa tcttcaccag ttttttattg atatcttcat
    26341 tattaatgga gacctctccc tgatgaggga taatatttaa cattgcttta agcaatgtgg
    26401 attttccagc accatttgga ccaataatac ctgttaaagc accttcttgt atttttaaag
    26461 aaatctcgtt caatgctaaa aaatcgttat aacaaacatt aagctttttc acttctaaca
    26521 taactttctc cttctaaatg ataatgatta tcacgatcat atggtataca aaaattatat
    26581 tttagtcaac cctaaatttt atagtttaga tttttttctg ttgctgaaga ttagaatgac
    26641 aaaactattt gaaactacgc gtatagtaag tagctttcaa aatatataaa aaaggagcgt
    26701 tctcaaatga gaacatgctc ctaaaactac tttattggcg tatggaaaaa ttatacttga
    26761 agtggatagc gggttgaata agttgataag catcaaaaca tttataaaaa ttattcaaga
    26821 ctatgtctgt tccatataca aatatgaaaa tcagtaaact ccatttaact tttttcgtag
    26881 tgtgtctttt tatacatgtt tatcgttatt aatagtgaat aggaaaaaaa ctatttttga
    26941 tcattgacaa ttaaatacaa tttttgaata acgatccctg ctattcgagc tagaaactca
    27001 agtacgccat gacctgatta gcgacaaata cttagagatt tctttttgtt ctgggaaaac
    27061 aaaatgccat gacaatagca gcaaataatg atacttccgt aaggctcgcc gtaaactggg
    27121 gagaggtgaa attcctatgt gcttacccaa agcaattcaa aaatttcata actactattt
    27181 taggagatga ttttatagaa tccagagtct acctgaataa aaaacaaacc tgtgcatttc
    27241 taggaatcga taataatact ctagataagt ggattcgtat atataatttc ccagtaataa
    27301 gagttggtgg tgtagttcgc tttgacgtag aagatattaa agactttatg aatacattta
    27361 aaataacttg aaattttctt ctcgccgcat atactaaaat tagtgaaata tactgcctta
    27421 ctttttttaa ggagtgagga ttttgcctac aaagctttca aatgggaaat ataaaactaa
    27481 tctgagatat ccaaaaaggt ttaaagaaat aacaggaatt gcatcagaaa agtatcaaaa
    27541 aacatttcca aataggcaat tggcgattaa agccgaaaat gatatgaaga agaaaataga
    27601 aaaagttttg agagaggaga atgcaaactc attagaatta aaaggaaaaa taacgtttaa
    27661 aaaattttac gaatctaaat ggcttcctag atatgaatta ggtcaaacaa ttagatcaaa
    27721 caggcctcct agtgatataa ccattagtaa tacaaaagat atatttcggt tgcatatttt
    27781 acctatgttt ggtgaatacg caatgaatta tctaaatatc aatactgaga taatatcaga
    27841 tgaattaacc aaaaaatcga aagaatatgc aaatataaaa atcataaaag gatatgttcg
    27901 ttccatgttc gatattgcag aaatactaaa ttatatcgaa tttaatcgaa ctactaaaat
    27961 tattcaaagt atcactgcac ctaaaaaaaa tgctttagaa gaaaaaagaa tacaagaagg
    28021 taagcaagca ctatcatcaa aagaacttac taattggata gaagcagtaa atgatgattt
    28081 taataatcat ttacttactt ttcatgatta cacacttttt atgattaccc tgtatcttgg
    28141 tgatagaaaa agtgagactt acgctttgca atggaaatat attgattttg aaaaacaaac
    28201 ggtccgtctt aaacatactt tagataaata tcaaagaaaa aagtttacaa aaggtcgaaa
    28261 agataccgtc attcaagtac cagaagttgt aatgacattg ctaagtgaat ggaaatcggt
    28321 acaagctgat caattgttga aattaaaaat taagcaaacg ttagatcagt atctctttac
    28381 ttatactaaa ccatctggag aagtaaattg tcctgttcat gccgattacc taaactatcg
    28441 aatcaattca attaaaagaa ggcatcctga cttagttcat ttatctccac acaaactccg
    28501 tcatacttat gcaactatag ctagacaagg tggagcagat atgaatcaaa tttcaaatgc
    28561 tttaacacac tcagatattt ctacaacaaa gatatatgtg aacacaccag atatagttga
    28621 taaggctgtt tttgaagctt ttcagagagg attgaataag tgtgattgat atgtgttctt
    28681 aaatgtgttc ttaaaaacaa aagaagcact ttccaaataa ttgaaaagtg ccagaaacgt
    28741 tgatatataa aggatattaa cgttttgaga attgtgaagc tttacgtgct ttcttaagac
    28801 ctggtttttt acgttcgacc atacgtgcgt cacgagtaag taagccagca cgtttaagag
    28861 ctaaacggaa gtcaggatca acttctagca atgcacgagc gataccatga cggatagctc
    28921 ctgattgtcc agcgtatcct ccgccgttta cgttaacaaa tacgtcatat gcgccttttg
    28981 tttcagtaac agcgaaaggt tgattgatta cttcacgcaa gtcagcatgt ggaatgtatt
    29041 cttcaacatc ttttttattt acagtaattt tgccagttcc tggtactaga cgtacgcgag
    29101 caactgcgtt tttacgacgg cctgtgccga tatattgaac ttgtgccaat gaaattccct
    29161 cctattagat taagtttgtg atgtctaaaa cttctggttg ttgtgctgca tgtggatgtt
    29221 ctgctccacc gtatacgttc aattttgtaa attgtttgcg gcctaaagtg tttttaggaa
    29281 gcataccttt tacagaagtt tcgatcaaac gacgagaatt tttagcacgc aattcgccag
    29341 cagtgattga tttcaatcct cctgggtagt tgctgtgacg gtaataaatt ttgtcagtcg
    29401 cttttttacc tgttaatttc actttgtctg cattgatgac gatgacgaaa tcgccagtat
    29461 ccaaatgagg tgtgaaagtt ggtttatttt taccacgtag tacagatgca acaactgttg
    29521 aaagacgtcc caatggaaca tcagttgcgt ctactacata ccatttacgt tctacttcgc
    29581 ctggtttggc catatatgtt gtacgcacgt tattttcctc caatttaagt tctgatgttt
    29641 gacatacact tcgagtttcc ggggctcttt gtggggcaaa caataccatt taacatatta
    29701 cccgctaatc tagtcaatgt caacgttttt ctgcttgatt gaaaaattta tatgaaactt
    29761 tacttctttt ctttctttta tataaaaggg tcgtttcctg tcaaaatgaa aaacaaaaag
    29821 aggctgtgac aaaagtcttg tcacaacccc ttattttgaa taaacggtgt tctcctgcca
    29881 actcctcggt cacaagtcac aatatccata aaacataaga agctgttttt taaaattgtt
    29941 tcttgtgact cggagctgaa cggcaggctt acaacctctt ggtaaacggt gttcaatcag
    30001 ctgaccctcg gtattagctc aaaaaacggc aaaatatgag gaactatttt gacgtttgtt
    30061 tctctaatac tcaggtcaaa acgctgattt cacaacctct ttttcggtca agttcaacaa
    30121 cagtttttac gcgtcgatct ctggcgcttt gcccaaatga gcttggatca tccagatacg
    30181 tttttctgtg gctgttttga attcgataaa gatatcttgt gttggatcgt cgccttcttc
    30241 tccagaaact tcgattcctt tttggtaaag atctgctaga taacgatagc ctgcaacaag
    30301 tgtttccaaa cgttctggaa tagtacgatc ccagcggcca atttcatcag ggatttttgt
    30361 gttatctgca aattcacgca atgtagaata aggtgaaccg tctaatgtga tcaagcgttc
    30421 ggatacttca tctaactgag cgttcaaatc atccatgaac tcatccatca ttgggtgcaa
    30481 tgtaaggaaa cctggtcctc tcatgtacca atgtgtttgg tgaacaacaa cagacatttg
    30541 gcttagatct gcaactaatt gatttaatac ttctttggtt tgttggaatt tcatctttct
    30601 tgcctcctaa cattttctta cacttaaaat aatagtagac tttcaatgaa aagtacaaca
    30661 aaatcattta cctgagaacg attttcagtt cgctttttta tccgtaaagc caatcatttt
    30721 tcagaatcag ctaattcttc tcgcagttga gcaaattgct gataagtctg tgctaaatgc
    30781 ttttgtgtcg aaacaaataa tggatagatt ttctgatatt ttttatgatc agtttcttgt
    30841 actccaactt gctctttcaa ttctatcaat tcctgtgctt cactgatact ttggatcttt
    30901 ccgatgcttc tccagcctaa aatcacagct cctaatgcgg atgcttctgt gacagtaggg
    30961 aaaacaagcg ggtaacccag tacatcagcc atcatctgac gaaaaatcgg agattgagca
    31021 aacccgcctg tagcacggat ctctgtgagc tgattttcct ggcaaactcc ttttaaaatc
    31081 cgataaaggt tgagacagat cccttctaga atcgccttca tcatttcttt ttctgtatgt
    31141 tgccgctgta aaccgataaa actgccaaac gcatctgcgt cccaaagcgg tgctcgctca
    31201 ccaagtaaat agggatgaaa aatcagacca ttggcacccg ctggcacaaa agcgatagcc
    31261 gaaagcagtg catcatatgg attttctcct ttttctttag cagattgaac gacttcctgt
    31321 aaaaatgtat gccctgccca atcaaagatg ccagcaccat tgctagtcgc tcctcccact
    31381 acccaatgtt tctgatccag aacataacaa aacgtctcca tttctggatg gaactgaggc
    31441 tgagtagtaa taaaacgaag tgctccgctc gttcctacgg tcaaagcggc gatcgtatca
    31501 ccaaaagcac ctaaccctag attagaaagc ggcccgtctg cccctcccaa aacaaatgat
    31561 atatcatttg gcaaacctag ttctgctgct gtttttgatg gtagacctgt tacttgatag
    31621 gtggaaggaa agacttgcgg caattgctct attcgaatgt tcaagtagtc aagcacttct
    31681 tctgcccatt ggaattcatg gatatcaaaa tatccagttc ctgaagctgt tccgtagtcg
    31741 ctgatcagtt cgccaaacaa tctataaaag atatagtctt tgataccgac aaaataagca
    31801 gtttttttga ataattcagg ttggctgttt tgcagccact tgattttgtt caatggtgtc
    31861 attggatgaa tcggcgtccc agtctgccga taaaattctt tgccaagcac ggtatatttc
    31921 agtttttctg cttcgtccgc tgctcggtta tctgcccaag tgatcattcg agataacggt
    31981 gttttctctt catccaacaa tatcaggcca tgcatcgcag aggagaagga gacaaattga
    32041 atgattgtgt ctgaaggcaa ctgtttaatt acttctttta taacggtgca gacaccttga
    32101 aagatcaatt cctgatcttg ttctgccatc ccttgtgttt cttggatcaa agggtagcga
    32161 aaataagcgg atgctgcttc ttgggcttca tctgtgaaag caactgcttt ggtttgtgtg
    32221 gttccgatat ctaatccgat tgaaacgttc attttttcac ctcaaatttt atgtatgtac
    32281 aaaaatttag tttaagcttg cgtatcttgg ttttgaaaag ggggagatgg cgtgttactt
    32341 tattttatca ttggttgctg tatcggatca tttttatgtt taaccgccca gcggattccg
    32401 ttaggtcact ctattattta tcctagatct cattgtgtca aatgctgtcg ttcgctttct
    32461 tggtatgaat tgattccgat tctctcgatc atcgtccaac gatttcgctg ccgctattgc
    32521 aggtgtcggt tgcccttcta ctatttgttg gcagaagcct tatgtggtgg tctttttgcc
    32581 tggttcttta ctttttcttc cgcaagaaat ttttcgacgt tcatatggat gctttcggct
    32641 ttgctatttt cgttgatgga tttattttac ttcatggtcc attctcacac gctattctgt
    32701 tcttgggcaa ttttatggat tttttggttg caggccggcg tcttccaatg gcaaagtgtg
    32761 ctgttaacgt ttatcgtagg cggtgtgtgc cttcgctatg gtcaatccta tctcggagct
    32821 ggcgatactc tgttgatgct ttcttggagt ggtggcttgt ctctagaaga gttgcttcag
    32881 atcttgtttc ttgctagtct gtttggaata tccgtttttt ctgtttactt tatctgtcat
    32941 aaaaaaaagt tggaaaagct gcctttcatt ccttttttga gcagtgcgtt gctgattgtc
    33001 cttcatctta ggtattgaaa aaaggttgtg acaaacgact tgtcacaacc tctgatatat
    33061 agtgttctct taccaaatcc ttgtgacttg gagctaggcg acaggttcac aacctctcca
    33121 ctaaatggtg ttcaatcagc tgaccctcag tattagctca aaatgctggt tttacaacct
    33181 ctgatatacg gtgttccata agtagctttt tcggcttctt atttcttgac gccccaatgt
    33241 cgacgaagga aacctgcacg tccagaacct tctcgataaa gattgtaatg gagcttgttt
    33301 ttcttgtaat aatcctgatg gtaatcttct gcaggataaa attcgcttct tggttcgatt
    33361 tttgtcacga ttggcttatc gaatcgcccg ctgtcttgca atgcttgttt tgaacgctct
    33421 gcaatatttc gttcttcttc atcattatag tagatcactg gacgataaga atcaccgcga
    33481 tcagcgaact gtcctaatgc gtcagtcgga tcggtttgct gccaatagat ttccaccaac
    33541 tgatcataag aaataacttc tggatcatat tcgatctcta ctgcttctgt atgccctgtt
    33601 gtgcctgttg ttacttgttc ataagttgga tttggtacat gcccgccagt ataaccggat
    33661 gtcacagaaa tgatccctgg ctgctcttca aatggtttga ccatacacca aaagcatccg
    33721 cctgcgaaaa ctgctttttt tgtcatataa aatccccctt ttttctcatc ataccttgat
    33781 ttccaaaagg aggataacgt tttgcttgtg acagaaaaaa cggaaactat tatatacgtg
    33841 atacggcttc tgataaatgc caaaacgaac gtacttgatc aaaaacggtt aaatcaggaa
    33901 tcacatattc agcagaagga agatttatgt tttcacctat tcccaataca tgcatgttac
    33961 cagccaaagc tgcttgacaa ccagcttctg aatcttcaat gaccaagcat gcatttggcg
    34021 gtacattcaa ttgctgtgct cctttgagaa aaacttctgg atcaggtttt gcttttgata
    34081 cttgcgtgcc gtcaataagg acatcaaaat aacttgtcaa attcgttttt tctaaaatga
    34141 ggcgtgcatt tttgcttgct gatcccaatc cgatcttgat gtttgttttt ttcaagtact
    34201 caagaacagc tattgcacca ggcaaaacgg atgtttcatc cattttttga atcgcttgaa
    34261 gatatagatt gtttttttgc tcagcaaagg ctgatttctc tttttctgag aatttctgtt
    34321 ccatttttcc aaatgacagc aatcgctcta gtgactcggt tctggatatt cctttcagtt
    34381 gctcgttttc tttctcagtg aatggaatgg acagctggtt agctaaaacc agccacgctt
    34441 cgtaatgata acgagctgtg tctacaagaa cgccatctaa atcgaatagt gctgctttga
    34501 ttttcatcga ttacctcctt gaaaatgaac gacttgaagt aaagatgcga tgtttttagc
    34561 tgtgcgatga agattatcag tggcttgctg taaagcttct tgtaaaggca cagctgcagg
    34621 aaggatcgga aaaacggccg aaatgccaac atctgctgct ttttcgcttc ctttaccaac
    34681 acttccgcaa atagctatca caggaacgcc ctctggtact cttctagcaa tcccaactgg
    34741 cgcttttcct tttagacttt gtccatctag acgtccttcg cctacgatta cgaggtctgc
    34801 ttgctgtacc cgtttatcga actccagtaa atccaatacg tagtcgatgc cagagactat
    34861 tttccctgaa gcaaatgcta acagtcctgc acccattcca ccaccagcac tactgcccga
    34921 tgcctcgatt acttgagggg caaatctcat ataaaaatca tgcatggctt ggtcagccac
    34981 tgctaattca ttttctttca agcctttttg tggtccgaaa acaaaagaag cgccttcttt
    35041 tccgcataaa agattagtaa catcagtaat gacagtgatt ttggctttaa tcagctcaat
    35101 aggcacgttc gttttatcaa ctgtagcaat tttacctaga ttttctccca gtgcttctac
    35161 tttttgatta tcttgatcgt aaaaagcata acctagacct tctgccatgc cgatgccgcc
    35221 atcgttggta ctacttccgc caacgccgat gatgatgtgt cgaacacctt gttgcaacaa
    35281 ttgtcgtatc agttctccta ctcctcgcgt ggataaccta agtggctgtt gttggtttag
    35341 tgggacatct ttcaatccga cgatttctgc catttcaaaa atcgcagttt gaccgtctga
    35401 agcgtactgg atttctttgt ttttgccaaa tgcattagta acgactgctg tttgccgctt
    35461 caaccttaaa ctgtctgcta atgatgaaac cgttccttct cctccatcgc ctataggtag
    35521 cagatcatat tcagcctgaa caaaaacttc ttgaaaacct gattgaatgg cttgtgctgc
    35581 ctctaaggct gtcagacttt ctttgaaaga atctggcgca atcagaatac gcattatttt
    35641 cccctccgtt ccaagatcag ttctttttcc agctggtatt ggtctccatt gacaaaaata
    35701 ggtaatgttt ctttcgtctc tttttttatt actatttgtt tttttgtcaa aacaaatagc
    35761 aacctgtgat ttttccaaat aaaaggaaaa gaaatttttt cccatgctga aggaagtctt
    35821 ggagaaatag aaagagttcc attcgttact gtaatacctg aaaaaccaaa tataactgct
    35881 agccatagtg ctcctaatga agcagcatga attccgtcat ctgatgaatg cggatctggt
    35941 cctaaatcaa tcaggcacgc ttcttggaat aaatgatacg cccattccat ttcttggata
    36001 cggcaagcct ctatggcgtg aatcgcttta ctcaacgatg agtcgtggat tgttttgttt
    36061 tcatagtaat gcaaattctt agaaacgatt ggttgagaaa aatactcagg taataagtat
    36121 aacagcatga caagatctgc ttgcttgctg acttggagtt catttacttc gctacgggca
    36181 taatcaagta aaatagactg gctgccagga cgttctttgt aagtggtcaa atcaataact
    36241 ggcttactga gatacgtgtc atcttgcgga atcagctgtt ctttgttagg cactggcaaa
    36301 tataacttat ctaaaaaatt ttgacattct ttcaaaaaaa gcggatcgcc agcttgatat
    36361 ttaacagcta aggccacatt ataatgcgcc agatagttcg tatatgcatt attgtcgata
    36421 tattctgtgt attcatctgg accaatgact ctgtgtatct ctaaatgatc atcgactttt
    36481 acagctctgc tcagccaaaa agctgctgtt tcatgaatga gcgtttttcc ctttgtttct
    36541 aaaaacgatt gatcctctgt tgcttggaca tactcttgaa cggcataagc aaggtctgca
    36601 ctgatatgat gttctgcggc agctgaagca acttcttgtc ttgttccggt tttgatattc
    36661 aatgcagcaa atttgggtgt ctcttcctga ccagtgtgtg cgctttccca tgggaaaagt
    36721 gcaccttgat agtgataatc tcttgctttt tgccgtgcgc cttctaatcg atcataacgg
    36781 tacagcagca aattctttgc tacttcagga aactgatata aaaagaaagg caggataaaa
    36841 atttccgtat cccagaaaac atgcccttta taaccttcgc cagttagtcc cttcgcccct
    36901 acactaacac ttggatcatt gcggggagtc ataatctgta aatggtaagc agcgaagtcc
    36961 atcgctaact gatcgaaaga agaatcgcct tggatcgtta tccgatgctg atgccaaaaa
    37021 tctgtccaat aagacatcga ctgctctaaa atttctgtat aactaacgct tttctgggct
    37081 tgggcaatac ttttcttaaa cgggtctgtt tgctgaagag aggtagagag cacgatgatc
    37141 ttctccagcg taaaggacgt attttttctg atcttttctg aaaaggaagc ggtgatccgg
    37201 cgattttttg cttggaatac acctgcttta ttgaatttag ctgcaatccc aatatcaatt
    37261 ttactttctg ttgtctggta ggatgcactg atcaaatcgt caccaaatac tcgcagttct
    37321 ttttcaacca actgctgtgt accaaaattg gtttgctgtc catcgatacc cgttttcaca
    37381 tctacttggc agtcttcggt caacggcttg atgaccactt tcagggctgc ctgatggaga
    37441 ttattttgat cagcaacacg acaagtaatg acttccactt tttttccttg agcagtttca
    37501 atgagcgcat agcgtttaag ctctcccatt tctaaatcta gtgcccgctc gtatgcaaga
    37561 actttcatcg tctcaagaga aaaatgttca ccgtcgattt ttatttccca tctgatgaca
    37621 tctgggagat tcactaactc ggctgattct gctcctaaag gtcggttata tacaccagct
    37681 gcaaacattc cccttacttg attccgatag tcttcctcct ggcttcctct gataccaaaa
    37741 aatccatttc caatggtcat ctgactcgca tacttattga tatactctaa atcaaactgt
    37801 ttttcttcta ctttcataag gtaataactt ctcctgtttc tgccgaacga tataaatttt
    37861 ccaccagatt ctggatgaca tatccttgcc agccatctgc aatcatctga gtctggtcac
    37921 cgagcgcccg attcacaaaa tgagccatac ttagttcatg tgggtcatta actggcgtgg
    37981 tcttttgtaa ggaaaccagc tttccatcta catcttcgaa tagttcgcct ggatacagat
    38041 tagcacctgc ctgatcgcca caaaaagcca cattcagttt gtcttctctg atattcaaag
    38101 caaacgaagt attcagttgg atgatcttgc cgttttctaa ttccagtgtg ccaaaaattg
    38161 catcttctac tgtgaatttg cttggatccc atttcccgaa tttccctgaa ttttttttcg
    38221 taccaatttt ttggaagctg tacgcccgaa cgctttttac tttaggaaat ccagtaatat
    38281 acatcgctgt atcaagcatg tgaatgccat aatcgatcaa aggcccgcct ccttgaagtt
    38341 ctttgtcgat aaacgttccc cagcctggaa caccagaccg acgaagcgca ttcgcttcta
    38401 cgtaataaat atccccgaga acatcaagat gattcttgat cattgttgct tcgtcagaaa
    38461 aacgatgctg gaatccataa gccaaaatct gcccgttttt atcggctgtc tctgccatct
    38521 ttcttgcttg atcagctgtc atagctggtg gtttctcgca catcactgaa gcattagctt
    38581 cgagggcagc caatacatgt tcgaagtgaa agcgattggg tgtacagaca ctcacgatat
    38641 cgggagaaat ctcattcagc atttgtgtaa ctgatgtata cgcatgagaa aatcctgtac
    38701 gtcggcaaaa gtcttctgct cgctggctga ctggatcaac cacagcggcc aattctaaga
    38761 catctgatct tttttgataa taagcagcat gtactttttc tgctacttgt ccagcgccta
    38821 tgattgctac tttcttcatt tatttctctc tcctttgctt atttacttat tgttattctt
    38881 tctctattct ttctctaaat catttaaatg acgcgctgtt tccagacagt ttttcaagta
    38941 ttgcaatgat tcctgataaa ttttcacttc attttctcct cgcaaacgac attcatatac
    39001 catataacct tcatactcat tttgaagtag cgtttgaaaa tggcgagtaa aatcaattgc
    39061 gccagatcct ggttggtagc gatgattatc tgctaaatgg atatgaccca catactcttt
    39121 ttgcgtagtc aatgcttttg aaatatcatc ttcttcaata ttcatatgat aaaaatcagc
    39181 gatgatctgc acatgtttca attgattaga gtcaatgtaa tgacgcgcat ccgctaacaa
    39241 attgatcata tggtcttggt atcggttcaa aggctccaaa taaataaccg tacctgtttc
    39301 ttcggctact ttatctaaat aacgaagtga ttcgctgact gctttatagt ctccttcttt
    39361 gcttcggggt gaaaccatcg gcggtaatcg ataagtgaac attccccaag ctgctgggat
    39421 aacgattcct ttgcctccaa cctcgcttag tgcacgcaaa atctcagcaa tttgacgtac
    39481 accattaaga cgccgttctt cgataaagtc accgatccac ccatcgtagc cgttacaagc
    39541 tgtcactact gggatccctg tttctttgat tgcctgtttg actgtgtcga tattttctat
    39601 taacaactta ccgtcgattt cgtattcttc aaatccacag tctttgataa atctaaattt
    39661 ttctaaaatg tctcttggga aaaatgcttg atcttgcgta ccgaatttca ttgttctcct
    39721 cctcggactt gaacgcccat tttgatactt tcttctgggt gctggtctac ataacgcatg
    39781 taagcttctg cgctttctgc aaatggcaca actggatcaa tgatttcttc acaatccaag
    39841 taaccgttca tcaataattc ccaacatgtg tcctcgattc gtttacggtt ccatcttggg
    39901 taatctgggt ttggttcact agatgctcga gcaaagacga tttttgcatt attgaaatgt
    39961 gcttcgcgac caagattaaa cccagctgga aaaggtttag caaaggcaac ataagaaatg
    40021 atcccgccat agccgatccc tattaaagaa gcttgtaatg cggcagcact gccactcgtt
    40081 tcaataactg aatctactcc acgtttatct gtcaattcct ttagacgaaa accgacatct
    40141 gctttcatag gatctagcgt gtaatcggca ccgtgttttt ctgctaattc tctacgctgc
    40201 gctatgggat cgatcccaac gatggtagta gcacctgctt ttttagctaa ttgaatcgca
    40261 atttgcccga tagcgcctaa accgaagatt gctacagcat cacctgctcg aacatgtgct
    40321 tcccggatac cacctaaagc gaattgagca ggatcgtaac atactgcatt tttccattga
    40381 tccaaccgat caagtttacg tagtttataa ttatcaacac cgtttgtaat cagcgtttca
    40441 cgaatgggtc cataagtgca gactaactct cctttttgat actctgtcac caaactgccc
    40501 gtttcttcga tcgtaccgac aatcatattt cctaattgaa attcgccaaa taccacaccg
    40561 cgttggcttc cttcttctct tggcaagaat aggcgccatt cttctgaaaa ttcttcatcg
    40621 ataaatggac ttaatcctct aaagtcgaca atttctgttc ctggttttgg agtagcgaaa
    40681 attactcgga tacgtacttc gttcttttcg atctcacggt cttcgtaatc tttgaatccg
    40741 gctattcttg gttctaatgc aactaatttt ttcatctttt ctttcctctt tcttttcttt
    40801 ttatcctttt acgccgcctt cgactaaatc acctttaata aatcgttcag ataacgcata
    40861 cataatgatg actggtaaag cggtcaccaa tgaagcggcc atcattcgtc cccaaatgta
    40921 gtctggtgta ctgaataatg tgttcaaacc gattggtaaa gtaaattttt cggaggtcga
    40981 caggaaaatc gaagcaaata aataatcatt ccacgaaacc ataaagcaat aaacgaagac
    41041 agaaatgatt ccagaaatag ccaatggtat aatgatccga ataatgatct ggatccggct
    41101 aagaccatcg atcattgctg cttcttcaat atcagtcgga atcgtgtcaa aataactttt
    41161 tagcatgaat atcgcagtag gaagtgtttg aacgaccatc gtgataatca gtgctgtatt
    41221 ggtatcatac aaacctagac cggaaatgat tttaaataat ggaacaacga tcaaaatacc
    41281 ggaaaacata taaacggtgt aaaagctagc attgattttc gagcgagctt tgaagcgtag
    41341 ttttgacaaa gcgtatgccc cgctgatccc aatcactact gcagttacgg aggctatcaa
    41401 ggagatcact aaactatttt tgaaatacat gatgaatgga aaaatctcag cattgaaaat
    41461 atcctgataa tgttcaaaag tccattcttt cggaaaaata gtttgtgtcg ttgcaattgc
    41521 ttcttgtgaa tccttgaatg atgtcatcag catgatcaaa aatggaaaca aattgacagc
    41581 taatagcaac aaaatcgtcg cgtaaaataa gattttcctg ctctttttat ttttcatttc
    41641 aagtccacct tctttcgtga gagcaagacg accgctgcaa taatgatgaa caatagaatc
    41701 gatatagctg ctgcttttcc aagatcattg aacgcaaagg ctgtctgata caaataaacg
    41761 cctaagatat tgactttatt cgtcagtaga tagacatctg taaacatata gaacatccag
    41821 atagatcgta aaacaatcac agttgctagt actggtttga ttgccggcag tgtcacgatc
    41881 ttgaacttct gccaagcatt tgcaccgtcc atttcagctg cttcataaag cgttttgtcg
    41941 actgtttgca aaatagccaa aaatgaaata aacgcatacg gatagtatct ccagatagca
    42001 aacaaaacga ccataaagaa gctagcaact ggctggtcga accacagcgg tgcttcgttg
    42061 aaaagctgca acctatctac taacagatca ttgacgattc cataactatt attgaacata
    42121 tatttccaag caaaaatcaa agaaatagat ggcgtaacat aacttaataa aatcagtgaa
    42181 cgcaccgttt tcctaaatct gaacttgcgg ttaagcaaaa tagctacggc taatcctaaa
    42241 gctgtactgc ctgctacaac taagaatgtg taagcaactg ttaaacctaa agaacgataa
    42301 aattcactat cacttaaaat ttcaagataa ttgcttaatc cgataaaaac ggcgtctgag
    42361 ttaggattca gcggcatatc gaaaaaactg atctgaatat tggaaagcat tggaatcagc
    42421 actaaaacag ctaataaaag catgcttggt gctaaaagtc ccatggctaa ttttaagtca
    42481 gaatgtttcc ttggacgatt cattgaaacc tccccctgat aatctgccaa tagctatcta
    42541 cttttttcga acaccattgg cagaacttct tgttatttcg tatttttttg tgcgtcagct
    42601 aaagcttgat cgacatcttc gtttccgaca gttacttggt tcactgcttt tccgatcaca
    42661 cctgagcttg tgatatctcc cattttcgtg aaattctttt ccccgactag tccaaagact
    42721 tgtacttcat caaaagcact agcaatctca gatggcagct cgccaaaaga cttgatgact
    42781 tcattttctt tgtaaccatc cagttcgact acttgtttat tcactggttg agcaccacca
    42841 ggagacatca agacccactt ttccatgttt ttcggttgag ataagaaagc tgtgaatgct
    42901 tcagcagcct ctttttgttt ctcgtctaga ccagaagaaa tcgtcaatcc agatactgtg
    42961 ccatagacag cttgatcttt ttgtgttggg atagcaaaac cgatattttc tgagttcccc
    43021 gcttcataaa cagaaggcaa aatgtaagtt gaatagattg ccattggtgc tgttccattc
    43081 atgaaagcat ctttgatttc tgtcacatca ttagagcctg gcatcgtgta ttgggataag
    43141 ttttgataaa acgacaatgc ttctctcatc ttttccgtat cgatcgttac ctcgccttca
    43201 tcatccaaaa catttgctcc atttgacaaa gcaaattgag agaacgcttg ctcactcatt
    43261 gttccttcag cagtaggcat agcaattcca tattgtttat tctctttatc tgtgaaatgt
    43321 tgagcaattt ttaaaatatc atcccaattt tctggttctg aaaaaccagc ttctgataat
    43381 ttttctttgt cataccaaat accttgaacc cagccgctaa ttggtgcagc aatatagctt
    43441 tttccatctt ctgaacgaac aaggtttttg gctccgtcat aataattatc ttctccagct
    43501 tcctcgataa cgttttggac tgccgtctga tcaatcagtt catctttatc catcacttta
    43561 gcaaaatcct gactgacttc cattacttct ggtaatttac cggaacgagc taacgtaacg
    43621 atttttgtat tgaacgctga ttcttctacc ggcacttgct tgattttgat gtctggattt
    43681 tctttttcaa aatcagcaac cagttcattg atcacactta atcgttcctg ttcgacagaa
    43741 gagtgcatga attgaatcgt tgtctgttgt gattcgttag agctttgact ttcaccacaa
    43801 gcagtaagaa gaaatccagt agctagcaga caggtagtaa caataatttt tttcattttt
    43861 ttccacctct tattttatta ttttcagcca catatattca tacggctgga atatcaactt
    43921 tcgttcttga ctgcaatgga taatttgtcc agaaaacagg tctaggtaat ctccttttgg
    43981 taaaagataa cctgctgatt gattggaaaa attatggata actaagactt tctcttcctt
    44041 cgcttcgcga ataaaactga taaaagaatc tgctgagtcc agtaagtaaa tcaaactatt
    44101 cggatggaag gctttttctt tttttcgaat agatagcatc tgggtcaatt caccatatac
    44161 tgctgttctt aatggatctg tctgcaactc tttctcaata tctgctaaca aaaatttttg
    44221 ccgattgatt tttcgattct ctcctgtttt ttctacgcct ttgtaatcat ttcgacttcc
    44281 cagcacactt tgaatataaa ttgccggcac gcctggtaac gttaccaaaa atgaatgagc
    44341 agctaaaaag cgtttgcttc tccattccgt cgtatctgtt ggagaactta acgcatctag
    44401 atacgtagcg tttatctcat atggactggt cgttccatct gggtttttct tgtagtttac
    44461 tcgggcaccc tctttcttca gctgttccac taattcaagg atcgtttttt ccggcagaat
    44521 acctcgtacc ggattcaatc cgataccgtc atgggaggct aaaaaattaa aaaaagttgt
    44581 ttttttcgat gcgaaatcga tggtttttaa ccattctttc aacttacttg tattacccga
    44641 acgaatcgct tgaagggtca aaggtggtag cgaaaactga tagaccatct gtgcttcatt
    44701 ctcgccattc ccaaaataac tgatattatc tttgtgtggc acatttgtct cagtgatcaa
    44761 taccgttcct ggagctgctt catcagcgat tgcacggaat aattggatga ttttatgcgt
    44821 ttctggcagg tgtatacaag aagttcctag ttctttccat aaaaatccca cagcgtccaa
    44881 gcgaataaat gaagcgcctt tttctaagta aaacaaaaga atatcgatca ttttcaataa
    44941 aacttctgga tttttaaaat tcaagtctac ttgatcttcg ctaaaagtcg tccatacgtg
    45001 tttttctttc ccgtttttgt caatgaacgt tgtaagtaac gggctggtcc ttggtctagt
    45061 cacgctgctt gtatctgtat ccagtggcaa atcaataaaa aaatctttgt acgtatcatt
    45121 caagtttaaa tattcctgaa accaagtact ttcggcagac atatgattgc agacaaagtc
    45181 gaacattaac cgtgtttcgc ttctcatttc ttctatatct tcccatgtgc ccaaacggtc
    45241 gttgattgcc tgataattta caacggagaa accatcatca gatgtatatg ggaaaaatgg
    45301 caagaaatgt acaactctaa atgtatctgc caaatacttt tgataaaatt tcttaaatgt
    45361 acgcaaagtt ggttgctgct cttcaaaaaa ttgatcccca taagtaatca agaaaatatc
    45421 ttcttcgtcc caatgcagtt tgcctgcctg gcctttcttc tgctgataaa cactgacttt
    45481 ttgttcaatc atagaggcgg ttttctcaga caaatctgag tcgcgataaa tcgcttgtaa
    45541 aagttttgta tacatcggat tcccctttct tttttgggag cgttcccaac gatttgatca
    45601 tattatggaa aacgcttact gtcaatagca tttttttaaa taattttgat atacacaaaa
    45661 atacaaataa gaaagcttat gtatcaataa ttaatagctt cttaaaaaaa tatggtctct
    45721 catgatcatg caagctattc ctaaattttc aaaatcatag acaaaaaaga atgtctattg
    45781 tactatggaa ttattctaaa acgctcccaa aaaggaggtg catataaaat tgaaacctac
    45841 tatttatgac attgcgaaaa aagctaatgt ctcaaaatca actgtctcca gagtattaaa
    45901 taaccagcca aatatctctg ataaagcaaa agcaagtgta cttcaagcaa tcaaagaatt
    45961 gaattatgaa ccgagcaaaa ttgcaagagg actgtcagga aatggttttg acgcgattct
    46021 agcattatct catcgtactt ctcatacgac ctatggcaat cctttttttt cagatgttat
    46081 tcaatccatt tctgccgttt ctgaacaaaa taacttcgac ttgattctac aaacggcaaa
    46141 aacaccagaa gaagagctag aaaaatgcgc gaaaaagatc caagaaaaaa tgatccgagg
    46201 aattattatt ttaagctcta cgatggatga aacacttctt tttgaattgg atcaatataa
    46261 tattcctatt gtggtaatcg gaaaagtcgg aaacgactac acgaacatct actcagtgga
    46321 cacggataat ttccaggata gttacgattt ggtggaaaca ctgatcaaga atggacaccg
    46381 tcatatcggc tgtctccatg ctccgacaga agtttatgta tccaatgatc gtctcaatgg
    46441 ttaccgtcaa tgcttatttg atcaccacct agatatccgt aatgaatgga ttatagacgg
    46501 cggctttcgc ttagaagata gcatggcagc aatgaatgat ttaatgaagc tacgagaaag
    46561 accaactgcg attttttcta ccgatgcatt aaaatcattg agtatttaca aagtggcaga
    46621 acaatctgga aagcagatac ctgatgatct ttcgatcgca ggattcagtg atcccttatt
    46681 gtcctcccta ttttcgccac agttgactcg gatagacatt ccgacaaatc agttaggaat
    46741 caaagccaca gaacttttat tccaactaat ccaccaccaa taccctgaag aaaaaagaat
    46801 cattctttca accgagatta ctctcacaga ttcgatccaa caacaagaaa tgtagaacag
    46861 aattataaaa gaacagaaac actgaggata agtcagttac tctgttcttt tttcaatcac
    46921 tcatccctcg ttaccccctc ccggtatttt agcgaatttt tcttattttc catctgtttt
    46981 agatagctgt ggataactat aatgtttgtg ttaaactatt ttttccacag ggaagaaaaa
    47041 actattttta accacgttgt gtataagttt ttatagttcg tttttttaaa cacatttacc
    47101 cttatttcaa tccatgttat ttggggatac ttatctctaa aacataaata gaataacaga
    47161 catgtctact ggttttgttt tattcgagga attattcaat aatagctagc aaaatattaa
    47221 aaataaccgc tctatcttcc catcaattta ttttgcattc aaaaaatgat ctatctttac
    47281 ttttgttgat tgacttctct tggaagatga tcgttccgat accttaattg gatacgaacc
    47341 gatgttgtta agatcctttc ccacttttct tctagttaga caaattattt tagacaaacc
    47401 aaaaaagagg ttgtgacaat aatttgtcac aacctctttt ttaaaatatc tattttgttt
    47461 gattgttcat tgcgtcttct gcagccattt gcgcttcaat atctgaaata ctgtagacat
    47521 tttcgcttgc tacaccgact tcttttggtt ccatgtcacg ataacgttgc ataccagtac
    47581 cagctgggat gatcttacct ataataacat tttctttcaa accaagaaga ggatctttct
    47641 taccgcggat cgcagcatcc gtcaagacac gagttgtttc ttggaatgat gcagcagata
    47701 agaaactatt tgtttctaat gatgcttttg tgatacctag taagacaggt cgagaagttg
    47761 caggaacgcc tcctgagact agcgtattgt agttttgatc tttaaagtct gcgatgtcta
    47821 ataatgtacc tggtaagata tctgtatcac ctggatccat tacacgaact ttacgcagca
    47881 tttgacgaac cattacttcg atatgcttgt cgccgatttc taccccttgc atacggtaaa
    47941 cacgttgtac ttcacgaagc aagtagtttt caacagataa tacatcgcga acttgcaaca
    48001 attgttttgg atcgattgat ccttctgtca atggtgcacc acggtggatc atatcgcctt
    48061 ctgctacttt catacgtgct gtataaggaa cagtatacgt acgtgtatct gtttttcctt
    48121 tgattgttac ttctttggta cgtgttgcag gatcttctga gatatcgatg acatcccctg
    48181 ttacttctgt gatcactgct tgccctttcg gattacgtgc ttcaaagatt tcttggacac
    48241 gaggaagacc ttgagtgata tcgtctccgg caaccccacc tgtatggaat gtacgcatcg
    48301 tcaactgagt accaggttca ccgattgatt gagcggcgat cgtaccaact gcttctccga
    48361 cttcaacgtc agagccagtt gcaaggttgc gtccgtaaca atgtttgcag acaccatgtt
    48421 ttgtattaca tgtgaagaca gaacggattg tcactgtttc gacacctgca tcaacgattt
    48481 gttttgcgat gtcttcggaa atcaattggt ttcctgcaat gatcaattca tttgtttcag
    48541 gatgacgtac ttctttacgg gtgtaacgtc ccactaaacg atcttctaat gattcgatga
    48601 tttcatttcc ctcgcggatg gcttggattt caagacctcg gtcagtaccg caatcttctt
    48661 cacggatgat tacatcttgg gccacgtcaa ccagacgacg agtcaagtaa cctgaatcgg
    48721 cagtcttcaa ggctgtatcg gtcatccctt tacgagcccc gtgagttgag ataaacattt
    48781 ccaagacgga tagtccttca cggaagttcg aaatgattgg caattccatg attcgaccat
    48841 taggagcggc catcaaacca cgcattccgg caagctgtgt aaagttggaa atgttaccac
    48901 gagctccaga atcagacatc atgaagattg gatttttcgc ttccaaacct tccatcagtt
    48961 tttgttggat gctgtctttt gcagcattcc aaacagcaat gacacgttca taacgttcgt
    49021 catctgtgat cagaccacga cggaattgtt ttgtaatagt ttcaacttgt ttatgggcat
    49081 tttcaatgat ttcttgtttt tcattcagta cactgatgtc agcgatccct actgtgatac
    49141 cggcatgtgt cgaatgtttg taccctaagt ctttcatacg gtcaagcatc ttagaagttt
    49201 ctgttacttt gaatcgtttg aagacttctg cgatgatgtt tcctaagttt ttcttcttga
    49261 acggtaatac taattcttgc tctttgatat gtgcaggaat atcagtacca gcttcaacaa
    49321 agtatttgtc cggtgtttgg acggtcaagt tgaagtctgt tggttcattc agataaggga
    49381 attcaggagg catgatctcg ttgaagatga tcttaccaac tgttgtgatc atcgtacgtt
    49441 ctttttgcca ttcagtaaat ggtttttcac ctaatgagtt agggttcaca ccgatacgtg
    49501 aatgtaaatg aacatatccg ttgcgccatg cgataacggc ttcgttttga tctctgaaga
    49561 ccattccttc gccttcgcgt ccttcttctt ccattgtcaa gtagtagtta cctagaacca
    49621 tatcttgaga aggagtaaca actggtttac catctttagg gttcaagatg ttttgagcag
    49681 ctaacatcaa catacgtgct tctgcttgtg cttcttcatt caatggaacg tggacagcca
    49741 tttggtctcc atcgaaatcg gcattataag cttcacatac aagtggatgc aaacggattg
    49801 cgcgtccttg tactaaaact ggttcgaatg cttggatacc aagtcggtga agcgtaggtg
    49861 cccggttcag caatactggg tgttccttga ttacgtcttc taaaacatcc catacttcgt
    49921 cttcttgacg ttcgatttta cgtttcgcat ttttgatgtt tgaagctagt tcgcgttgaa
    49981 caagctcacg catcacgaat ggtttgaata attcaatggc catttctttt ggaagtccac
    50041 attggtacat cttcaagaaa ggtccaacaa cgataacgga acgacctgag tagtcgacac
    50101 gtttacctag caagttttga cggaaacgac cttgtttccc tttcaacata tgagaaagag
    50161 atttcaatgg gcggttacct ggtccagtaa ctggacgtcc gcgacgaccg ttatcgatca
    50221 atgcatctac tgcttcttgc agcatacgtt tttcattttg aacgatgatt ccaggtgcat
    50281 tcaagtctaa caaacgtttc aaacggttat tacggttgat tacacgacgg taaagatcat
    50341 tcaagtcact tgttgcaaaa cgtccacctt ctaattgaac cattgggcgt aaatctggtg
    50401 gaataacagg gataacgtcc ataaccatcc agcttggttg atttcctgaa gcgcggaatg
    50461 cttctaaaat atccaaacgg cggattgcac gtgtacgttt ttgtccttgt gcagatttca
    50521 attcttcttt caattcggct gcttctgcat ctagatcaac attatcaagt aattgtttaa
    50581 tcgcttctgc acccatagca gcttggaatt cttgtccata ttgttcacgt ttttcgcgat
    50641 attcgcgttc tgtcaacaat tgttttttct ctaaattcgt atttcctggt tcgatgacta
    50701 catatgaagc aaaataaatg atttcttcca aagcgcgtgg gctcatgtct aatacaagac
    50761 ccatacgtga aggaatccct ttaaagtacc agatatgtga aacaggagct gctaattcaa
    50821 tatgtcccat gcgttcacgg cgaactttcg aacgagtaac ttccactccg catcgatcac
    50881 aaacgattcc tttataacgg atacgtttgt attttccgca ggcacattcc cagtcttttg
    50941 taggaccaaa aatccgctca cagaacaacc cttcacgttc aggttttaag gtacgatagt
    51001 taatcgtctc aggttttttt acttcaccgt aagaccagct tctgattttt tcgggagaag
    51061 ccaaacctat ttgcatactt tcgaatttat ttacatcgat caaaaggggt tccctccatt
    51121 tcatttaatg gcaaaccgca agacggctga tcttcgaccg ccttgcagct catagaccgg
    51181 attagtctaa attatcttct gcggtttcaa agttttggat gacatcgtct ttttcatctt
    51241 ccacttgttc agcagctttc ttttctaatt ctttggcagt ttgctgttcg gcaaattttg
    51301 tcaaagcatc aacagtgatc aaatcgtcgt cttcatcgtc catgtcgcga agttcgatct
    51361 ctgtttcttc gatatccaag acacgcatgt ccagacctag tgattgcaat tctttcacca
    51421 atacgcggaa tgattccggt acacctggtt ttggaattgg ttcacccttg acgattgctt
    51481 cgtatgtttt cacacgtccg actacgtcat ctgatttgta tgtcaagatt tcttgtaaag
    51541 tataagccgc accgtaagct tccagtgccc atacttccat ttcaccaaaa cgttgtccac
    51601 caaattgagc tttacctccc aatggttgtt gtgtaaccaa tgagtaaggt ccgattgaac
    51661 gtgcatgcaa tttgtcatcg accatgtgag caagtttgat catgtacatc acaccgacag
    51721 acacacgtcc atcaaatggt tcacctgtac gtccgtcgta cagaacagtt ttcgcatcgc
    51781 tggccatacc ggcttcacga actgtttccc atacgtcttc atcacttgca ccatcaaata
    51841 ctggcgttgc tacgtggata cctaactgac gtgcagccat acccaagtgt aattccaata
    51901 cttgcccgat gttcatacgt gaaggcaccc ctaaaggatt cagcatgata tcgattggtg
    51961 ttccatcagg taggaatggc atatcttctt ccggcataat acgggataca acccctttat
    52021 ttccgtgacg accggccatt ttatcccctt catggatttt acgtttttga acgatataaa
    52081 cacgaactaa catgtttacg cctggtgaca attcatctcc agcttcacga gtaaagattt
    52141 tcacatcgtg aacgataccg ccgccgccat gtggtacacg taatgatgta tcgcggactt
    52201 cacgagcttt ttcgccaaag atcgcatgca atagacgttc ttctgcagat aattctgtta
    52261 ccccttttgg cgtgactttc cctacaagca aatcgccatc ttggacttct gcaccaatac
    52321 ggatgatacc catttcgtct aaatctttta atgcgtcttc cccgacatta gggatttcgc
    52381 gtgtgatttc ttcaggtcct aattttgtat cgcgggcttc tgattcatat tcttcaatat
    52441 ggatagaagt gtatacgtca tcttttacca aacgacggct catgatgatc gcatcttcat
    52501 agttgtaccc ttcccaagtc atgaaaccaa ctaaaacgtt ttggcctaga gccatttcgc
    52561 cttgttccat agatggtccg tctgccagag tatccccttt ttcgactttc tcacccaaat
    52621 gaacgattgg acgttggttg tagcttgttc cggagtttga acgacggaat tttgttacat
    52681 cgtatttatc taatgcaccg ttgtcgcggc gtacgcggat ttcagaagca tcaacgtatt
    52741 caacgacacc gtcatgttta cataacaaag cagctcctga gtcatgcgct gatttgtatt
    52801 ccataccagt accaacccat ggtgcttgag gattgatcaa cggcactgct tgacgctgca
    52861 tgttggcacc catcaaggca cggttggagt catcgttttc caagaaagga atacatgctg
    52921 tcgcaacggc aactacttgt ttaggtgata cgtccatgta gtctacttta tcaatagata
    52981 cttctaagtt ttcactttga gcacgagcca taacaacgtc ttgagcaaat gttccatctt
    53041 cattcaatgg gctgtttgct tgggctacga tgtaatggtc ttcgatatca gctgtcaagt
    53101 agtcgatttg gtcagtaaca cgacctgttt cacggtctac acgacgataa ggtgtttcga
    53161 taaatccgaa tttatttact ttcgcataac tggacaaact attgatcaac ccgatgtttg
    53221 ggccttcagg agtttcgatc ggacacatac ggccatagtg ggagtagtga acgtcacgaa
    53281 cttcataacc ggcgcggtca cgagtcaatc cgccaggtcc taaggctgaa agacgacgtt
    53341 tgtgtgtcaa ttcgccaaga gggtttgttt gatccatgaa ctgtgacaac tgagaagaac
    53401 caaagaattc tttgatactt gccacaactg gacggatatt gatcaattgt tgaggtgtca
    53461 atgtttcagt atcttggatc gacattcttt cacgaaccac acgttccata cgtgctagcc
    53521 cgatacggaa ttggttttgt aaaagttcac cgactgaacg gatacgacga tttcctaagt
    53581 ggtcgatatc atcgacattg ccgatacctt ccatcaagtt caagaaatag ctcattgaag
    53641 caatgatgtc ggctggacga accgttttta cagctgcttc tggatagccg tttccgatca
    53701 cgtttacttc acgttcaggg tctcttggtg aaaatacttt gatgacttgg acagtcattg
    53761 gatctgtgac cacaccgtct tctgatggat agtaagtcac tgaatttagg ccattatcaa
    53821 tgaaaggagc tagtgtttcc atgacttggt gagtcagaac tgttcctttt tcaacgatga
    53881 tctcgcctgt ttctggatca actaatgttt cagctaatgt cagattcaat agacgtgttt
    53941 tcaaatctaa ttttttgttt actttgtaac gaccaacgtt tgctaagtcg taacgttttg
    54001 gatcgaagaa acgagcattc agcaagttac gtgagctgtc tgctgttttt ggttcgcctg
    54061 gacgcaaacg ttcgtaaacg tctttcaaac cttcttctgt tcttgagtcg cttgcgtttt
    54121 tatgcaagtc tttttcgact gtattgcgta atgtttcgct gtcgccgaag atttcaaaga
    54181 tcgtatcatc ggaaccaaat cctaacgcac gaactaatac ggttaaagga atcttacgtg
    54241 tacggtcgat acgtacataa gagatgtctt ttgcatcagt ttccatttct agccaagctc
    54301 cacggttagg gataacagta gaaccgaatc cttcttttcc gtttttgtcg acttttccat
    54361 ggaaatagac acctggtgaa cggactaact gagaaacgat tactcgctct gccccgttga
    54421 tgatgaatgt tccttgttct gtcattaatg ggaaatcacc gaagaatact tcttgtgctt
    54481 tgatttcgcc agtctcgcgg tttgtcaaac gcaatgttac atgtaatggt gcagagtagt
    54541 ttgcatcatg cgcacgagct tctgctactg tatactttgg ttctttcaat tcataatcta
    54601 caaattccaa agatagattg ccattgaagt catcgattgg caaaatgtct tcaaacattt
    54661 ctctaagtcc ctcatctaag aaccattggt aagagtctgt ttgaatttcg atcaaatttg
    54721 gtaattccaa cacttcactg attcttgcga aacttctacg ttcgcgatgt tttccgtatt
    54781 ttactacgtg tccagccaag ctcttcaccc ctctaaaatg tcattcaaaa agactacccg
    54841 atcaattgtt acaataatat ttcaaatatt atttttaggt aacaacagac attttttgcg
    54901 gcttttaggc aaaaaaaaac caaaaataga acattctctt gaaaccttac ttcgtgagct
    54961 tctacttttg tttccttata ttaaagccga aacggacaat atttcgtttc tgccttttct
    55021 tcatcagata gtttttgcaa cttcatatac tacacttatt ttctataaaa gtcaacactt
    55081 cctggcgttt cttctgcttt ttcgcaacat tttcttaatg ttttcaattt tatgttttca
    55141 attatctgac aattcttaat tgacagacct ttttatctcg gttatacttt agaaaattca
    55201 ttttgtggag gaacttctat gcgaaccaac atcaaccaaa tcgcaccaac acaactgaat
    55261 aacttttact ttagctactt tagataggtt tgtattcatc atgatggata caaacgttaa
    55321 acgacagttt gtatctatga tggctaaaga actttgtatt gcagcaaaaa gccacgtaga
    55381 caaaatgcta agtggctgag cagtgaaaat acaaagtaaa agtatcaagc attctacttt
    55441 ttcaatttat tttccgactt ttcaatccat ttggacattt tgtctgaatg gattttttgt
    55501 ttcccaaaat taaggaggaa aaagaaatga aaaaaaatat aatcatcgga ttaggcgctg
    55561 ccctgctact agctttagga ggttgcgcac aaaaagacaa ttccgcagag gcatcagatg
    55621 gacaaaaaac aattggcgta ttgcaacttg tacaacataa ctcgttagat gctgcctata
    55681 aaggattcaa agaaggatta gcagaagaag ggtataaaga aggcgataat ctaacaatcg
    55741 attatcaaaa tgcccaaaac aatcaagaca acctgaaaag tatgagtgag aaactcgtca
    55801 aagaagatcc tgatttgcta cttggaatcg ccactccagc agcacaatct ttattaaatg
    55861 aaacaaccac tatcccgatg actgtcacag cggtcacaga cttggaacaa gcccatttag
    55921 tcacatccaa tgaaaaacct ggcggcaatg tgactggcac gacagatatg gtgccaatcg
    55981 aaaaacaaat cggcttactg ttaaatatcg tgccagacgc caaaacaatc ggcatcatgt
    56041 ataacaacgg tgaagcaaac tctaagatcc aagctgattt ggcagaaaaa gaattgaaaa
    56101 aagcgggcgt caaagtcaaa gtactgacag caaattccac aaatgatgtc caacaagtga
    56161 cgaaaagtct agctaaagat gtagatggaa tctatattcc tactgataac acgttcgcaa
    56221 gtgcggcaag tgttgtcggt gaagtcgcaa aagaaacaaa aacacctgtc gttgctggcg
    56281 cagttgatca agtcaaagac ggcggtctag caacagtagg aatcgactac gaaagcttgg
    56341 gcaaacaaac agggatcatg gcagcaaaaa tcttagacgg caagaatcca agtgaacttc
    56401 ctgtcgaaac agcgaatgat ttggaactgt atgtcaataa agacatggcc aaagcattag
    56461 gcatcaatcc agacagcatc aaagaagctg aataaaacac aggaggattc tcatgacaaa
    56521 tttgctctct cttttcttat catctgcatc gcaaggggtc ttatgggccc tacttgcgat
    56581 tggtgtatac gtcactttcc gtatcttgga cattgccgac ttgactgctg aaggaagctt
    56641 tcctttaggt gcagggatcg cggcagtaag tattacaaat ggctatcatc cattgacagc
    56701 ttgtcttcta ggctttttag ggggtgcggc agcagggctt gtttccggac tgcttcacac
    56761 aaaattgaaa attcccgccc ttcttgcagg gatcatcacc atgactggtt tatattctgt
    56821 cacatcccgt gtgatgggtg cgccgaacat ctctttattg ggtcagccaa cgatctttca
    56881 ttgggtcgag tcattcggca taacaaaaga caacgctgta ctgatcgtcg gtctgctttt
    56941 tgctttgatc gttgtatgtt tacttgtttt gttcttacgt acagaaacag gacttgctat
    57001 tcgtgcaact ggcgataatc tggcaatgag cgaagcaaac gggatcaaca cagataatat
    57061 gaagatcatc ggttatatga tttctaatgg atgcattgct ctttctggct ccttacttgc
    57121 gcaaaacaac ggctttgctg atttaaattc tggtattgga acgatcgtaa ttggacttgc
    57181 ttctattatc atcgcagaag ttttgttacg taaccagcca ctatttctcc gtctcttgac
    57241 tgtcgtttta ggtgcggtca tttatcgttt tattttagcg ttagtcttcg aactgaatgt
    57301 tgaaccaagt gattcgaaac ttgcttctgc tttcgtgcta gtgatctgtc tttctttccc
    57361 gcagatccag caaaaattcc atttacctca aaaaaagaaa ggcggtagtg tcaaatgaat
    57421 gacgtcttaa cattggaaaa tctccatcat acctttgaaa aaggaacgat caacgaaaat
    57481 catgtcctga aaggcattaa tctttcctta gatcccggcg acttcgtgac gatcatcggt
    57541 ggaaatgggg ctggtaaatc tactttgctg aacagcatcg ccggtacatt ttctgtcgat
    57601 cagggtcaaa ttttattgaa tggaaaaaat atcacgaaaa aatctgttgt cgaacgttcg
    57661 aaatccatca gtcgcgtatt ccaagatcct aaactaggga cagccgttcg tttaacggta
    57721 gaagaaaatc ttgctcttgc tatgaaacga ggaaaaaaac gcggcttctt ccgtggtgtc
    57781 aaaccacaag accgaacatt tttcaaagac cagctggttc ggctaaattt ggggttggaa
    57841 aatcgtttat cctcagaaat cggtttgctg tctggaggac aacgtcaagc tattacgtta
    57901 ttgatggcca cattacagcg tccagaactg atcttattag atgaacacac tgcagcgctt
    57961 gaccctgcta cttccaaaac ggtgatggaa ttgacagaaa agctgatcac tgagcagaat
    58021 ctcactgctt ttatggtcac tcatgacatg gaagacgcga tccgctatgg gagtcgactg
    58081 atcatgctgc atcaaggaag gatcgtcgta gatatccagc aagaggaaaa acagcagctc
    58141 acagtgcccc aattattagc gttgttcaaa caaaacagcg gcacggcatt aaaagacgat
    58201 caagtacttt tatcttcata atcagaaaga ggatgggaca aaaaatgaac gcccaccctc
    58261 ttcttttttc tctttccttc tttcgttttt gctttatgta tcaaccgttg tttcttcgaa
    58321 aactattttg ttctctcctt cttcctgata gaatggatgg gaaaggagct tgatgccatg
    58381 acaacgaaag acaaggtact tcaattattg aaaaagacac cagattttct ttccggagaa
    58441 aagttggcac agcaactgga agtttcccgg acaagtattt ggaaagccat caaagaattg
    58501 gaaaaggcag gctatcgttt tgagcacggg cctacagggt atcgttacct gccctctgac
    58561 gtactagatg caaaagaaat ccagcaagag ctacggtcgt ttattcctga tttgaaagta
    58621 caaacgatgt tgacttctga atctacgatg aaagatgcca aactggcagc agctcaagga
    58681 gattctgttt cgaagttatt tgttgctgat acacaagaac aagctcgtgg acgttttgga
    58741 cggccttttt tcgctgaacc tggacgagga atctacatga gtctcttgct ccatccagac
    58801 aaacggtttg atgaattgcc tcaatatacg atcatcgcag ccagtgcctg tgtgaaagcc
    58861 attgaatctg taaccggtcg ttctgcttcg atcaaatggg tcaatgatat ctacatggat
    58921 ggaaaaaaag tctgtgggat tttatctgaa gcagtcagtg atatggaaag cggcaggatc
    58981 agtcatatca tcattgggat cgggcttaat ttttctgttc ctcaaaatca ttttccagct
    59041 gatctccaag aaaaagccat ctctgtcttt cccgacggac aagctagtgt cacaaggaat
    59101 cagctaatca gcaaaatctg gcaagaattg ttcacttatc tttctcaccc agaaaaagcg
    59161 ttggacttct ataaagaaaa atcattcgta ttgggaaaaa ccgtgtcgtt caatcaaaaa
    59221 ggaacgactt atgtcggtct tgcaaaagaa atcggtgatc atggcgaact gatcgtccaa
    59281 acaaatgaac agaagatgag tttgtcttct ggcgaaatca gtctcacatc tatccgtacg
    59341 caataattat cagatttcac aaaaggacac ctgatctcat acagtgtcct tttttctgaa
    59401 ttctcttcaa aagactctcc ttattctatc agctgacagt ttctctatcc tctagaaaaa
    59461 ggtattctaa aaatatctat aaagcatggt acactttctt taaatgaatc aatgcaaaaa
    59521 acaagagagt catcagttta cacgatcaaa agagatagca ggccgcttcc tttttcaatc
    59581 ttatagatag accatgggta acgataaaaa caaaaaagaa cttgcgactt attttgccgt
    59641 aagttctttt ttagctagtg ccttaatctc ttaatcccaa caaacgtctc gcatagatca
    59701 cctgaccaga gtgcatagca gcatcatcaa taatagaaac gagacgcatt cctcgtgtga
    59761 ctggtggtgt ccagttctcg tcaattactt catctagctc ggaaggttcc agccttctta
    59821 agtaagaaat agcaaaatca gcagctcctc gcaagtatcc tagcagatct tctatttgat
    59881 tagcttggat ctgctttgct tgttccagac tatgcttcca atcttgttcg ttatctgcta
    59941 tcgtcatcgg aaaccggctt tcccagtctt gactatgcca aattggagaa gttcctgcta
    60001 aatcagcaat ctggaaatct aattctcgag cggtatgcca agccagccaa gtcattgatt
    60061 tgatctgcgg ggctctatct tccactggaa aacgattggc ctcctcaata gagacgcctt
    60121 caaacaaacg ttcaaaccga tcgtatgccc gatccacact ctcgcatagc atatctaaat
    60181 aatccattta ttttcctcct ttttatttat cctacttact ttttcctaat tctaggatac
    60241 ctacttatat gttactacac acccttattt tttacacttt aacagtcacc atcatcaata
    60301 gtttggagat tgaaagaaaa agatagtgtc gaactatcaa ttgaacaaga agcaacaata
    60361 tgaacgtttc attaatattc tttaaataaa caatgatttt tcaaacgaac agtttaagac
    60421 taggagaaaa agagtataat aggaaaatgt ttgaaaggaa aggaagggga aaaatgaaaa
    60481 aaagggcaag tttactagtc ctagttgctt tgttgttcaa tatcttttcg tatggtgtgg
    60541 ccgtttcagc ggaaacatac attcctaaag accctgggaa tatttataat gatctaactg
    60601 gtaactatgg cttgttagga attgctagtc aattccacgt atttgctaaa aataagacaa
    60661 ctgtgaatgc acatacagac ggaaatatag caacacatga acttgatgca aaaaataatt
    60721 ttggtacaga gcttcatgaa ggtttattga accaagaaat aaattatata caaaaaactg
    60781 attctttcgc taattcttca ggcataccta caggtagcac aagaatgaat aaatttgtcg
    60841 ttggcagtaa cgtggatgta gcacttcaag atggtcatcc tacagtcaat ggatccaggc
    60901 ttgatcactt aactccagat gatttttata aagatcgtgc tgggcatgaa tatattgatt
    60961 tcgagcagga atttgctaaa ttgaatgtag cagcaaatga tcttgcaacg atcactccag
    61021 ctaaaacata tactgctgca gattttccag acatgaacaa tcggacaatc gatttaactg
    61081 atttatcaga taccgggttt ttattggtaa atatcgatgc agaagtgtta acgatgaata
    61141 cgccacttca aatcattaat ccaaatgatc aagtagttgt atttaatgtc atcaacagtt
    61201 cttctgcatt gaacgtacag tcaccaatca aatacaatga tcgttcaaat catgaaacag
    61261 aagatttttc ggatgcgaat atctcttgga attttggaaa tgagatgact gatttgacaa
    61321 tcagtgctcc ttttcaagga acaatccttg cgcctaatgc tactatccgc attactcaaa
    61381 accaagacgg aacgattata ggaacaaatg tgattttaga tgcagctacc aatcgttggg
    61441 atcctaacga aatatttatc agcgacaatg gtacagatac gacagacaca agcgacagta
    61501 cagatacatc taccacaagt gatagctcag attcatca
//
LOCUS       DAYYFU010000009        61366 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000009 DAYYFU010000000
VERSION     DAYYFU010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.009"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..563)
                     /locus_tag="ACHA34_000714"
     CDS             complement(<1..563)
                     /locus_tag="ACHA34_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFM8791384.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="ACHA34_000715"
     CDS             complement(750..1400)
                     /locus_tag="ACHA34_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791385.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACHA34_000716"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACHA34_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFM8791386.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /gene="yvcK"
                     /locus_tag="ACHA34_000717"
     CDS             complement(2912..3910)
                     /gene="yvcK"
                     /locus_tag="ACHA34_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFM8791387.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACHA34_000718"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACHA34_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFM8791388.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="ACHA34_000719"
     CDS             complement(4926..5657)
                     /locus_tag="ACHA34_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFM8791389.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="ACHA34_000720"
     CDS             complement(5661..6926)
                     /locus_tag="ACHA34_000720"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFM8791390.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACHA34_000721"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACHA34_000721"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFM8791391.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACHA34_000722"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACHA34_000722"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFM8791392.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="ACHA34_000723"
     CDS             12268..13770
                     /locus_tag="ACHA34_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM8791393.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="ACHA34_000724"
     CDS             13772..14509
                     /locus_tag="ACHA34_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8791394.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14554..14727)
                     /locus_tag="ACHA34_000725"
     CDS             complement(14554..14727)
                     /locus_tag="ACHA34_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791395.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14739..15368
                     /locus_tag="ACHA34_000726"
     CDS             14739..15368
                     /locus_tag="ACHA34_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8791396.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            complement(15450..16616)
                     /gene="dnaJ"
                     /locus_tag="ACHA34_000727"
     CDS             complement(15450..16616)
                     /gene="dnaJ"
                     /locus_tag="ACHA34_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFM8791397.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(16767..18596)
                     /gene="dnaK"
                     /locus_tag="ACHA34_000728"
     CDS             complement(16767..18596)
                     /gene="dnaK"
                     /locus_tag="ACHA34_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFM8791398.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(18647..19210)
                     /gene="grpE"
                     /locus_tag="ACHA34_000729"
     CDS             complement(18647..19210)
                     /gene="grpE"
                     /locus_tag="ACHA34_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFM8791399.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(19304..20347)
                     /gene="hrcA"
                     /locus_tag="ACHA34_000730"
     CDS             complement(19304..20347)
                     /gene="hrcA"
                     /locus_tag="ACHA34_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFM8791400.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(20581..21747)
                     /gene="hemW"
                     /locus_tag="ACHA34_000731"
     CDS             complement(20581..21747)
                     /gene="hemW"
                     /locus_tag="ACHA34_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFM8791401.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(21763..21990)
                     /locus_tag="ACHA34_000732"
     CDS             complement(21763..21990)
                     /locus_tag="ACHA34_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFM8791402.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            22474..23081
                     /locus_tag="ACHA34_000733"
                     /pseudo
     CDS             22474..23081
                     /locus_tag="ACHA34_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            23254..23967
                     /locus_tag="ACHA34_000734"
     CDS             23254..23967
                     /locus_tag="ACHA34_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791403.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(24082..25092)
                     /locus_tag="ACHA34_000735"
     CDS             complement(24082..25092)
                     /locus_tag="ACHA34_000735"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFM8791404.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25129..25611)
                     /locus_tag="ACHA34_000736"
     CDS             complement(25129..25611)
                     /locus_tag="ACHA34_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFM8791405.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            25761..26129
                     /locus_tag="ACHA34_000737"
     CDS             25761..26129
                     /locus_tag="ACHA34_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFM8791406.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            26594..27046
                     /locus_tag="ACHA34_000738"
     CDS             26594..27046
                     /locus_tag="ACHA34_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8791407.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(27170..28021)
                     /locus_tag="ACHA34_000739"
     CDS             complement(27170..28021)
                     /locus_tag="ACHA34_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM8791408.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(28034..28819)
                     /locus_tag="ACHA34_000740"
     CDS             complement(28034..28819)
                     /locus_tag="ACHA34_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8791409.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(29171..30112)
                     /gene="ribF"
                     /locus_tag="ACHA34_000741"
     CDS             complement(29171..30112)
                     /gene="ribF"
                     /locus_tag="ACHA34_000741"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFM8791410.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(30116..31039)
                     /gene="truB"
                     /locus_tag="ACHA34_000742"
     CDS             complement(30116..31039)
                     /gene="truB"
                     /locus_tag="ACHA34_000742"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFM8791411.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(31407..33769)
                     /locus_tag="ACHA34_000743"
                     /pseudo
     CDS             complement(31407..33769)
                     /locus_tag="ACHA34_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            33934..34390
                     /locus_tag="ACHA34_000744"
                     /pseudo
     CDS             33934..34390
                     /locus_tag="ACHA34_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            complement(34547..34894)
                     /gene="rbfA"
                     /locus_tag="ACHA34_000745"
     CDS             complement(34547..34894)
                     /gene="rbfA"
                     /locus_tag="ACHA34_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFM8791412.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(34921..37227)
                     /gene="infB"
                     /locus_tag="ACHA34_000746"
     CDS             complement(34921..37227)
                     /gene="infB"
                     /locus_tag="ACHA34_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFM8791413.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(37240..37551)
                     /locus_tag="ACHA34_000747"
     CDS             complement(37240..37551)
                     /locus_tag="ACHA34_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFM8791414.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(37548..37841)
                     /locus_tag="ACHA34_000748"
     CDS             complement(37548..37841)
                     /locus_tag="ACHA34_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFM8791415.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(37863..39038)
                     /gene="nusA"
                     /locus_tag="ACHA34_000749"
     CDS             complement(37863..39038)
                     /gene="nusA"
                     /locus_tag="ACHA34_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFM8791416.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(39061..39534)
                     /gene="rimP"
                     /locus_tag="ACHA34_000750"
     CDS             complement(39061..39534)
                     /gene="rimP"
                     /locus_tag="ACHA34_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFM8791417.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(39679..44037)
                     /locus_tag="ACHA34_000751"
     CDS             complement(39679..44037)
                     /locus_tag="ACHA34_000751"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFM8791418.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(44249..45958)
                     /locus_tag="ACHA34_000752"
     CDS             complement(44249..45958)
                     /locus_tag="ACHA34_000752"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFM8791419.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(46026..47294)
                     /gene="rseP"
                     /locus_tag="ACHA34_000753"
     CDS             complement(46026..47294)
                     /gene="rseP"
                     /locus_tag="ACHA34_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFM8791420.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(47455..48255)
                     /locus_tag="ACHA34_000754"
     CDS             complement(47455..48255)
                     /locus_tag="ACHA34_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFM8791421.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(48252..49064)
                     /locus_tag="ACHA34_000755"
     CDS             complement(48252..49064)
                     /locus_tag="ACHA34_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFM8791422.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(49259..49816)
                     /gene="frr"
                     /locus_tag="ACHA34_000756"
     CDS             complement(49259..49816)
                     /gene="frr"
                     /locus_tag="ACHA34_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFM8791423.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(49819..50541)
                     /gene="pyrH"
                     /locus_tag="ACHA34_000757"
     CDS             complement(49819..50541)
                     /gene="pyrH"
                     /locus_tag="ACHA34_000757"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFM8791424.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(50677..51558)
                     /gene="tsf"
                     /locus_tag="ACHA34_000758"
     CDS             complement(50677..51558)
                     /gene="tsf"
                     /locus_tag="ACHA34_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFM8791425.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(51657..52439)
                     /gene="rpsB"
                     /locus_tag="ACHA34_000759"
     CDS             complement(51657..52439)
                     /gene="rpsB"
                     /locus_tag="ACHA34_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFM8791426.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            52798..53277
                     /locus_tag="ACHA34_000760"
     CDS             52798..53277
                     /locus_tag="ACHA34_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8791427.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            53494..54585
                     /gene="phnW"
                     /locus_tag="ACHA34_000761"
     CDS             53494..54585
                     /gene="phnW"
                     /locus_tag="ACHA34_000761"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFM8791428.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            54578..54706
                     /locus_tag="ACHA34_000762"
     CDS             54578..54706
                     /locus_tag="ACHA34_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFM8791429.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            54710..55255
                     /locus_tag="ACHA34_000763"
     CDS             54710..55255
                     /locus_tag="ACHA34_000763"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFM8791430.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(55709..57400)
                     /gene="argS"
                     /locus_tag="ACHA34_000764"
     CDS             complement(55709..57400)
                     /gene="argS"
                     /locus_tag="ACHA34_000764"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFM8791431.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(57820..58767)
                     /gene="arcC"
                     /locus_tag="ACHA34_000765"
     CDS             complement(57820..58767)
                     /gene="arcC"
                     /locus_tag="ACHA34_000765"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFM8791432.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(58882..59901)
                     /gene="argF"
                     /locus_tag="ACHA34_000766"
     CDS             complement(58882..59901)
                     /gene="argF"
                     /locus_tag="ACHA34_000766"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFM8791433.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(59992..61221)
                     /gene="arcA"
                     /locus_tag="ACHA34_000767"
     CDS             complement(59992..61221)
                     /gene="arcA"
                     /locus_tag="ACHA34_000767"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFM8791434.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT        17058 a        13103 c        10623 g        20582 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc gctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacatttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaat aaaaaccaaa
    14521 aatgcacggt cgccatgaag ggaccgtgta tttttatctt cttattttct cacctaatcg
    14581 tatcagccgc atcgcttgtt ttcctgcaca aacaagcaat cgctctacct gttccatcgc
    14641 ataagaaagt accatcggtt cattgatgat atcaagtaca tagtcgatac caccctcact
    14701 taattgagct tgatttttct tctccatatt atgtagttgt gggacaactt tctgacaatc
    14761 agcaattgtg tcaagaacgg atcaatcctt tattggagtt gctcgaaagg gtcttctctt
    14821 attacggaca ggctttgctc actgtacata gacaacaaat cattatccta gtcaatcgga
    14881 tttccagagc ctcattgcta tcattgcttg ataaaatcca gacaaagttt aataaaatgt
    14941 atcagttaca gctaaacttt ggaattgggt cgctttgtta tacagagcaa gagacaccgc
    15001 aatctttcct tcatgccaaa caagtttgcg aatggatagc tttccatcaa tctgtgaacg
    15061 agatccgctt ttttgaagat ttagacctcg ggatcgtttt accagcaatc ccgtcagatc
    15121 aacgaacatt atatgtaaaa cgtattttga aaagcctgac agaagaagaa gtacatttgt
    15181 tcaagaagac tttggcatgc ttcagtaaaa ataacggaag catcaaaaat tgctcagaag
    15241 aattatttat tcataaaaac accttgcaat atcgattgaa caagttccat tctctcactg
    15301 gttatacccc tagaaactat gacgattatc atattctaaa gctggctttt ttgttggtac
    15361 aaacatgaca actacaaaaa aaccaggcga acatatgatt gttcgtctgg tttttaatta
    15421 ttaatgactt agcgttttta ctagaaaaac tacttccgct ttttcccgcc aaaagcatct
    15481 ttaaacttat cgaagaaacc gtcttcttgc tgttcttcaa ctttgatgcc gccagcttca
    15541 gcgaatgcac gtaatgcttc tctttgttgt tcatttagat ttttcggtgt gatcagtttt
    15601 actttgacat gttggtcgcc tgttgcacca ccacgtaact tcggagcgcc ttttccacgt
    15661 aaacggaagt tcgtacctgt ctgtgttcca gcagggatct tcaatttcac atctccatgg
    15721 acagttggta cttggacttc atcccccaaa gctgcttgta caaaacttaa aggcaattca
    15781 tagtaaattt cagaaccttc acgatcaaag atatcacttt cttctacacg gaagaccaca
    15841 tacaaatcac caaatggtcc gccgttttct ccagcttctc cttgaccagc taaacgcatt
    15901 tgttgtcctt cttctacacc agctggtaca ttcactttaa ctgaatgtgc tttcttctca
    15961 tgtcctgagc catgacaggt tggacaaggt tctttgattt ctttccctgt cccatgacaa
    16021 acatcacaag tttgacggct catcactcgt ccaagtggtg tttggcgttc tacattaatc
    16081 gtacctgcac catgacattt atgacaagta gttggttgtg tgcctggttt tgcaccgttc
    16141 ccaccacaag tatgacagat ttcttcccga ttatacttga tttctttttc tacaccaaaa
    16201 atcgcttctt caaaagacaa gtcgaccgta tattgcaaat cagcaccttg acgaggcgca
    16261 tttggatcga ctgaccgacc gcctcctcca aagaaagagt cgaagatatc ttcaaaacca
    16321 ccgaagccgc caccagagaa gccgccgaat ccaccaaatc cgccaccagc tccaccagca
    16381 ccgtaatttg gatctgtgcc tgcatgcccg tattggtcat atgcagcgcg tttttgcggg
    16441 tcacttaaaa tctcatatgc ttctgatact tctttgaatt tttcttccgc atcaggttct
    16501 ttattgatgt caggatggta ttttttcgaa agcttacgat aagctttctt tatttcatct
    16561 tctgatgctc cttttgagag ccctaagact tcataataat cacgttttgt tgccataggc
    16621 tttcctccta ttggttctca tcattttcac gtcgcttgtt atcatatcat attttgtttg
    16681 aaacaaaaac cgatacttca aactattttt caaaagaaaa agccaaagta gcaactttgg
    16741 ccttctctcg aaattcagtg aactgattat ttatcgtcgc catcgacttc ttcaaaatca
    16801 gcatcaacga catcatctgc tccgccttga gctgcttctg ggttttcttg agcttgttgt
    16861 tgtgccgctt gttcgtatag tttaacagtc aagttttgaa cgatttcgtt caatgcatcg
    16921 cgtttagttt tcatttcttc gatgttgttt gcttcaacag cggctttcaa ttcatcgcga
    16981 gctgtttctg cttttttcac ttcttcttcg tccactttgc cttctaattc tttcaatgtt
    17041 ttgtccacag agaatagcaa tgcatctaca tcattacgta ggtcaacttc ctctttacga
    17101 gctttatctg cttctgcatt tgcttctgca tcttttacca tacgttcgat ttcttcgtct
    17161 gttagacctg aagaagattt gatcgtgatt ttttgttctt tttgtgtacc tagatctttc
    17221 gcagatacat ttacaatccc gtttttatca atatcgaatg ttacttcgat ttgtggaata
    17281 ccacgaggag ctgctggaat atctgtcaat tggaatcttc ctagagtttt gttgtcagca
    17341 gccattgggc gttcaccttg taatacatgg atgtctactg ctggttgatt atctgccgca
    17401 gttgagaata cttgtgattt acttgttgga atcgttgtat tgcggtcaat caatttagtg
    17461 aacactccac ccattgtttc gatacctaat gacaatggtg taacgtcaag taaaacaaca
    17521 tctttcacat caccagtgat cacgccacct tggatagcag cacccatagc aactacttca
    17581 tcagggttaa ctgatttgtt tggttctttt cctgtttctt tacgtactgc ttctacaact
    17641 gcagggatac gagtagatcc accgactaaa ataacttcgt cgatttctga ttgtgataaa
    17701 cctgcatctt ttagcgcttg acgtactggt acttttgtac gttctactaa gtcagctgtt
    17761 aattcatcaa attttgcacg tgtcaatgtc atttccaaat gcaatggtcc agattctcct
    17821 gctgtgataa atggcaagct gatttgtgtg cttgatacgc cagataaatc ttttttcgct
    17881 ttttcagcag cgtctttcaa gcgttgtaaa gccattttgt cttgagacaa gtcaatgctg
    17941 ttttcttttt tgaattctgc taccatgtag tcgatgattt tgttatcaaa gtcgtcccca
    18001 cctagatggt tgtcgccagc tgttgacaat acatcaaaga caccgtctcc tagttcaagg
    18061 atagatacgt caaaagtacc gccaccaagg tcgaatacta agattttttc gtcgcgatct
    18121 gttttatcta aaccataggc aagagcagct gcagttggtt cattgacgat acgttctact
    18181 tctagaccag cgatcttacc agcatctttt gttgcttgac gttgtgcatc attaaagtaa
    18241 gctggcacag tgataactgc tttttcgact ttttcaccta aatagtcttc tgcgaatcct
    18301 ttgatatatt gaaggatcat tgctgagatt tcttgtggtg tatatgattt accttctaca
    18361 tctactttgt aacctgcttc accgatatgg cgtttgattg atgaaatcgt atttgggttt
    18421 gtcacagctt gacgttttgc aacttcccct acttggattt ctccattttt gaatgataca
    18481 acagaaggtg ttgtacggtt tccttctggg ttagcgatga tttttgcttc gtttccttcc
    18541 aatactgcta ctgcagagtt agtcgtacct aagtcaatac cgataatttt actcataatt
    18601 aacgtctcct tctttaattg aaagtgtaag ttttatatcc gcacaattat tgtgcaacaa
    18661 tgaccatggt tggtcttaac acgcgatcat gtaatttgta gccttcttgt agtacttcta
    18721 caatcgtatc ggctggtgta tcttcagtcg ctggaacagt ttgtactgct tgatgaagat
    18781 taggatcaaa ggcttctcct ttagcaggga ttttttcaat tccttcttct tccagtgcgt
    18841 tacgcaaact ttccaagacc atttccacgc cttttttcaa acctgcccct tgatcatctg
    18901 acacttctgt ggctaatgca cgttccaggt tgtcaattga tggaagtaat tttttggcta
    18961 aatcttgtga acggtatttt tgtaattgct cacgctcatt ttttcctcga tttgccatat
    19021 ttgcgatttc agcacgagcc cgcaagtatt tatcttccat ttcttctaac tcagctttta
    19081 atttcgctgt ttcgaattct tccgcttcag cttctgaaat acccgaatct tctgcagctt
    19141 cgacatcgat ttccggttct ggctgagcat ctgtcatttc tttatctagt tcttcttgat
    19201 tttctttcac gatacagtct tcctttcatt attcagtcag ctgtttgatt gctcttacta
    19261 agaaagagag aacgatcagc tgcaaatagc ttcgttattc acattacgag tttgacaagt
    19321 caagcgaacg gtagtaatca gcaagtttag ctgctagttc tcttcgataa acatcgacta
    19381 gaccgaacat ccgagaataa ggcatactag taggccctaa caaggctatt gtacctcttc
    19441 catgaccatc tatctcgtaa cttgcttgaa tcatactcat attctgcaat aaatcattgc
    19501 ctaattcagc accaatcttg atatgaatat tgctatccat cggagtgatc atttgtgtaa
    19561 gtccgtctga gtctttcata aaagaaaaca tcgatttgaa ctgatcaaga tcttgacggg
    19621 gctcataatc caagagattc atttgtccac tgacataaac cttatcttca aaaacatgac
    19681 caagaacggt gtcaaataac tgagacatcc cttctgtcgt ttggaaatat ttatgaagaa
    19741 tcatcggaat ctccgtacga agtttatggt acaccgtcat cagaggatct ccgactagac
    19801 ggtcgtttac aatctgtacc attttttcta gttcctgact tcccatatat tcgggaagag
    19861 tgaacacttg gctttctaca ttccccatat ctgttacgac gatagcaata atctgtcgat
    19921 tattcaaggg tacgatacga aagccagtca gtcggcggtc ttttatttca ggtcccaatg
    19981 aaaaagtcgt atagcttgtc agctcagata atatctcagc agattgctta ataatctcat
    20041 tgatttcatg aaattctctg cctagagact gacgaatcag cagcaaatca tcttctgcta
    20101 cgcgagcagg ttttaacaaa tgatccacgt aataacgata gcctaaagca gatggaatcc
    20161 gcccagaaga agagtgagtc ttcaacagca atccttcatc ttctaatgct ttcatatcat
    20221 tgcgaatcgt tgctgagctg gccttgactc cctcagacat caatgttttg gaaccaacgg
    20281 gcacgccact actcgtatag tgttggatga tcagacgtaa aatgtcgttt tgtcgctttg
    20341 ttaacatctt cctcacctct cattagcact catatattct aagtgctaat ccacttataa
    20401 atatagcaag aagcccttct cctgtcaaga gaaagactcc attttttagc actctaaaca
    20461 caagagtgct aattctaaat aattgatgta aatccagtta tatcaacgtt tcaatagtcc
    20521 tttattgtat ttttgaactt tttttattta aattcagata aaaaacaaat attctttaaa
    20581 ctatttttca aacaaaaagc gctcaaacac attgttcccc aaaaaggtcc ctcttggtgt
    20641 caaataaaca cggtcttctt cattaacaag taacccttct tgctctaatt cccttagcac
    20701 atcaccgtac accgattcaa ttgttttttg atagcgatca tagaaatgtt ttttagtaat
    20761 ccctgttttt tttcttagtc ctaagaacat ttcttcttct atttgatttt ttcgtgtcaa
    20821 tttttcttct tccaataccg ggcgttcatt tgctcgtaat gggcgtaaat agtgttggat
    20881 tggtcctttg ttgcggtaac gaatatctcc aagataaccg ctagcaccag ctccaaaccc
    20941 aaaatagtgg tcattgttcc agtacatgag gttatgttga gattcatgtc cggagagccc
    21001 aaagttactt atttcatatt ggtgacgtcc atgcttttcc attgcttcaa tcgtctcttc
    21061 aaacattctg gtttccgttt cctgatcagg cagttcgagt cttccttgtc gtacccagtt
    21121 catgaacatt gttttgtttt cgaggatcaa tgagtacaaa gaatagtgag gcagatccaa
    21181 agccaaagct ttttccaaag tatccagata tccttctagt gtttgtcccg gtaatgcata
    21241 aatcaaatcg atgcttacat tggtaaaatt ttctgcttct aaaaattgca tagtttgata
    21301 cacatcttct gcggtgtgtt tacgtccaat ttttttcaat aagcgattat caaatgtctg
    21361 cactcccatg gataaacgat tgacgccata gtttttcatt acttgaagtt tttcacgggt
    21421 caaatcacct ggattcgctt ctacagtaaa ctcattacca ttttgaaaag gcaggatctc
    21481 tctagcacct gataacagtc gatcaagttg tttagcagat aaagaagttg gtgtcccgcc
    21541 ccctacgtaa agtgtttcta aattcgctgt cggatgactt tctagcatta ttttcatttc
    21601 attcagcagc atttccacat actcatctac tggctgccct tctaaaaaaa ctttattaaa
    21661 atcacagtaa tagcagatat gctcgcaaaa tgggatatga atgtacgcag aaaccttgtt
    21721 ttctgcggga tttattgttt ctatcattga aattctttct ccttacgtcg aaaagtcgtg
    21781 cctgttttcg accttcttaa aatcgcgttc aagcaccaac tcattgaaat aagccgcata
    21841 tttagaatca aggaaaccta gctctaaaaa tgcttgctta ttgtcatcgg agctacacgg
    21901 tgcttttaca gcctctcgat aaacggtaat cgcaaagctg accttcggca ttaagtcaaa
    21961 atctgcaaaa atatgaaaag ctattttcac agattcctcc ttaagtgctc agaccacaac
    22021 gctttgctta caacctctca tatacgcata ctaaacgtat ttggcgaaca aatccactaa
    22081 aaaatccaag aatcgacttc ttggatgatt ttttctttca ttaaactggt tacctagtta
    22141 tatatctcta gggttgtttt catgtctgag tgcccgatat gatttatttt tcgtttctat
    22201 aattactttt tgccttccag tttcttttaa tattgtactc aagcaagtgg gaattggttc
    22261 ttttgtcatc catgtatgca aaatgttata atgctggtaa acaaaaatga ttacctgtca
    22321 tttttattta gttcgtattt aattaagttg actatctgta tactctgtct aaaacttaag
    22381 aattgttgtt ttcgctgtcg ctacccaatt ttaatgaatg tctgtaggtt aaaactgttg
    22441 tccttgttat agatagtaaa ggagagcttg aaaatgaaaa ctgcgattac gtttctatta
    22501 gaggaatatg ccgattggga aggtgcttat attactagca cactaaacca aaatgagaaa
    22561 tggcaaaaca aaactgcttc tatccaatca gaggtcactt ccattggagg attaaaaaca
    22621 aaaatcgatt acttaatcga acaaatacct gaaaaagttg acctttttat tatgatcggt
    22681 ggcaattcat gggacataaa aaatcatcat ttatatcaaa taatcgataa attgttaaaa
    22741 acagacgtac cagtagctgc tatttgtggg gcagttgatt atttagctca gagcggatta
    22801 ttgcaaggtt acaaacacac cggaaatgcg caatttcctt ggttagaatc ggaaaattat
    22861 cagaataaag aagattttat agaacaacaa gctgtgaaag atcgtaattt gattactgcg
    22921 aatggaacag ctcctataga attcacagac ttgattcttc aagcagtcca ttttgaaaca
    22981 gaagaaataa gagaaaaaac gattcgtcta tacaaacttg gattctatga ttactataaa
    23041 aagtacggag atcctttctc ctaatcattc tcttcaatta aatactcctt atcaatagtg
    23101 tgacaagtaa ttttactgat gattatttgt cacacttttt ctgacattta tcaaagaaga
    23161 tacactctat ttatattcat agacaaggtt ttacggattc aaaaattcag gattatgcta
    23221 tactggtatt aaaaaaataa tgaggtattt tatatgaaaa agttaggttt tattttattg
    23281 ttggtctctt tattccttgt aggatgtacg tctcctagta aagaaaatag tgatccatct
    23341 gcgaccacag acaaaagttc aatcagacaa accagttcag ctgaaaaatc aagtactcag
    23401 aaatcagctt ctactgaaac gaatacatct tcaacaagca gtaaagtctc agagcccaat
    23461 acttcttcta catctagtag tacagttgag acaaatattt cttcttcaac aatagacaca
    23521 acctcctcac agtcttctca gtcaatagat ccgttagcag gatattctga tctacaaatt
    23581 gagtacgcac gtgtttggct agctattaga ggtaatactt ttagagatac atcgggtgat
    23641 ttcgaattac atgttattca ttatcctgca ggaacaccta tcgcccctta tgatgcaggg
    23701 agtgcagtct atccgaaaga tgtggttatg ctcactggga aatacagtta ccagggagtt
    23761 ttagtgtatt caagtaacca tgacgggaca atcactagtt atccagttcc ttcacattgg
    23821 caaatgcctg cagatcaaac atcttatcca gcgttcatta gagaaacaac tcaagaaatc
    23881 attgataatg catctgtagt agctattcct acaggaaatc ccaatgacgt gaaacagtta
    23941 atcgatgtga cagttataga taattagact ttctaatatg ttatacaaaa agaagctgac
    24001 agcattttgt cagcttcttt cactttcttt tctatttaga tttattggtc tttgatagac
    24061 tccaaggcag gaaaaaattt tttaaataaa cgtttgcaaa acagtcgctt ttctatcttc
    24121 taccgctact tctgcatagg caccattaat caatgtcagt tgtggcggta catgaccaca
    24181 atcaatatca tacaagatcg gtagttttaa ttcctttgac agatcttcgt atacatcctt
    24241 cattttataa tctaacaaat cctctgccgc acttctaccg aacatgatcc ccgagctgcc
    24301 atcaaaccag cctgccagtt tcatctgaac aagtgatctt cttaaatcaa ctgacgatag
    24361 ctcacagttt tctagaaacc acaagatcgg ctttccatca ttatactttc ttttaaaaga
    24421 ttggacatct ccataaggag taccgattaa atgcctgatc gtatcaatac aaccacctaa
    24481 taatcgtccc ttcatcctta ctgcatctcc gctgactgat tgccaatttg ttggttcagt
    24541 gagatggaaa acagaagatg atgaatgctc atggggccac actttttgat acttttcaga
    24601 agattgctgt agaatcgatt ctccttctct tgttttcagt acactttccc acattgctgt
    24661 cgtttcatcc aagtactctc ctcttaaatc aataaggtta gttccatgtg cagtggctat
    24721 ccctgttttt aatgtgacag caagtagcaa cgtactaata tctgaataac ctactatcca
    24781 ttttggttgt agagcatcaa aatcgacaaa atcgagaatc tcaatcaata attctcctcc
    24841 ccaagtcgga aagatcattt ggatattttc atcttgcatc atttgattga attcatctgc
    24901 tcgttttctt gctggacctg atttttcctt atcttgcgtc caaagcgtct ttccagtaac
    24961 tacttggaaa gatttttctt ccattctctt taaagctgat tcgagtaacg aatggtgttc
    25021 ctctgatacg cctgaggatg gtgcagtaac cccaatcgtt gcatttcttt ctaataatgg
    25081 ataacttatc atgttgcttc acctccaagt atgcactatt ttacttggtt aattgttttt
    25141 ttcgtttgcc ggcatcaaaa atatcggcat ctcaagtagc tggatgattt tgtttaagtc
    25201 aaccgtttgg atgaccatcg tttgatctgg tctacctggt gcaaaaacaa gtttttcatt
    25261 tgataagagc tcttcatcaa taataatcgg aatctgtttt tcataaccaa acggagaaac
    25321 agcgccctgc ttttgtcccg tttgttcttc catgcgttct gatgaaacaa tagaaagcct
    25381 tttaccgacg atcttcccta atgctttgaa atccgtttgt ttcgtagtaa aagtcagata
    25441 tacgtagtta ttatcttttt tatcttttaa gaataagctt ttggtctctg tacctgtaaa
    25501 tccttgttct ttctttatta ccaaagcatc ttcatttgta taaatggctc gatgatgata
    25561 caactcaaaa tatatatttt cgtgtaagaa tttctttgcc aagtcaatca tttttatgtc
    25621 tcttttctat tttattattt gagacatctt atcatataaa tatagcggtt acaacattac
    25681 ttgtgttttg ttcgtttttt atacataact attgtttttc tggtaaaata aatcaaacca
    25741 cttaatggag gcgttttaga atggaaaaat ttcagccgat catcaatcaa atcaaagaaa
    25801 aagaacatca acaaaagatc aaagaccttt ttgctaagat aacagaaacg tatccccagc
    25861 tagacacaac tgtaaaatgg aaccaaccta tgtttactga ccatggtacc tttattattg
    25921 catttagttt tgctaaaaat catttttcta tcgcaccgga aaaagcagcg attcgtgccc
    25981 ttgaaaaaaa tatccaagaa gcaggctacg tatatacgga taacgtcatc aaagttcctt
    26041 ggaaaagtgt aatcaactgg gaattgattg aacaattgat ttcatttaat attgaagata
    26101 aaaaaggtca tgataaattt tggcgataaa gctttatacg aataaacctt ttactggcga
    26161 ggaaactatt catcacctta tacaggtttt gttcaatcct atccttttta tcatccctgg
    26221 ttaaacactt aattgacaaa gtatctgcta tttttataag gagtctttct tgtgctgaaa
    26281 aaaatcactg attgaatggc tttgaatttt ctacatagta aaattatttt ttcagcaatt
    26341 ttcctgcaaa acttttgaat aaggaaaaaa tacgagaaaa tatcagtgat ctttcataat
    26401 actgcccatt attttcaggt atatttaaat ataatatcac tttaggagag aacgaatttc
    26461 tttagagaaa taaaagttag aattttctga cttgattttt acaatgacct taattaaaat
    26521 cgttctttct acatttcgtg attaagcaaa tatcaactaa gtcagtagaa ccattcacat
    26581 aggagggtga acattgaaag atttttactc agtaaatgaa ttagccgaac aattaggtgt
    26641 tactacacgt tctatcagaa attatcttca tgaaggaaaa ctaaaaggaa caaaggttgg
    26701 cgggcaatgg aaattttctg aacgtaatct ttttgagttc ctatacggag atcaagcaga
    26761 ggaagcagca aaagaaatgc agcgattcat gctaaatgca ccaatcacga tgcgtttcaa
    26821 ccttcaatat cgtgatttta cagcaatcaa tcaattccga gaacaattag tccagtacca
    26881 taacgatgtt tacgcaaata aaaaagaccg tcttttacaa tacgatcttt ataaagacaa
    26941 tcatgcggag atactaatcg gtggtaactt caattatgtc gtaaacttta gtcaatggat
    27001 caatgaaaaa ttattgatcc agacagatat ttcccttgta tcttaaacaa ttaaaaagcg
    27061 ctagaaattc cagagatgtt ggaaattcta gcgctatttt tttataagta cttactgttt
    27121 ggttcaaaca aatatttttt taataaatga aattttacca tctttatcct caaataaaga
    27181 ccgtgaccat gcttcctact acgatcagca ataatccgat aatcgttaat tttttttctt
    27241 ttggtgtctt attttcatgc aggatgtaca taccgcctaa tgtagagata actacgttta
    27301 gctgtgtaat gatatatgca gtagcaaccc cattttcatt tgctgaaaaa atataagcca
    27361 atgctgacac actaaagatg gctccaacga tcgttgcttt ccaactcttg tcttcagaaa
    27421 atgcggttgg tttacggaaa atcacataaa tcactgcccc taaaaagaca ccgatcattt
    27481 gcggaaagaa aatggaaagt ccgcttgcat caactagttt aggcaatgca ctgtataccc
    27541 aataaccaat acttgttgat gctaatagac taattccttt tgtgataccg cctttttttc
    27601 caggatcatc actgattgcc gttaaagcag aaccaaatac aagaagaatg attgctgctg
    27661 ctccaatgat tttccccata cttccagacc attctccaaa agcgaagaca ccaatcaacg
    27721 aggttccgac taactgtaat gcagtcgaaa ttggcatcgt tttagaaaca ccaatcaagt
    27781 tgaaagatgt atactgtcct gtttgtccaa taacccaaaa tgcaccagaa agtaaactaa
    27841 tcaaaaatgt agtcactgaa ataccagctg gagaagcgaa taattgaacg agtaagccga
    27901 caagcaccgc tccaatacca gttcctaata tctcatttgt tggtctgcca ccaatctttt
    27961 tcaaaatcag tggctggatc ccccagccaa ctgccgggat caatgcaatc aataaaccca
    28021 tttttattca cccttatcct tttccacctt ggaaagaagg atataaagtc atcccaccat
    28081 ctgcaaatag tgtaatacct gtaacataac tggattcgtc tgaagccaac catgcagctg
    28141 tcgccgctat ttcttttggt tcacctacgc gtcctagtgg gatcatactt tccaagagtt
    28201 ctttttgttc aggatcatca aattttgccg cattaatagg tgtacggata gcaccagggc
    28261 ctatactatt gattctgatt cccttcggtg catactccat tgctaccgtt tcggtaaata
    28321 attttacccc gccttttgaa gcagcgtaat gagcaaacgt tggccatggg atctgttcat
    28381 gaacagaaga catattgatg atatttcctt ttttattatt ctctgtaaag taatttaaag
    28441 ccgctttcgt tccgataaaa acgccagtca aattgacttt taacactctt tcccaatctt
    28501 caaggctaag ttcatgggtt ggatgctgat tttccatccc agcattatta acccaaacgt
    28561 ccagtgtccc aaaattttct aaagccgtat caagtaaacg ctgtattcct tcttcagaac
    28621 tgacatctgc ttgtacagag actgcttttc cgcctgcctc ttcgattgct ttgactgctt
    28681 tttctgctcc ttctttatcc gagttatagt tgacaactac attcattttt tctttcccga
    28741 atcgttgagc aatcgctgtt ccaatcccct ttgagccacc agttactact gcgactttgt
    28801 tttctaattc cttgtacata gtacccactc ctttttcata cctaaacctg ttattacgga
    28861 taacttttat ttccgcctta aagtgagtgt acaccaagat atatagtcta aagcaaatga
    28921 tacgctctag caatcgtttt catgaattgc tagtgtcctt ttatttctat aaagaaagag
    28981 gttgtgacag aagtggtcag cttcaagaaa tttcggctta tttccgaaga agctacttct
    29041 gaaaaaccat ttatttggaa aaaaaggctg tgacaagact tttgtcacag cccctttcct
    29101 gggcttattt atcacccatt ttattgtaat tgctagttat acataaacaa gagattttat
    29161 gcataatgtt ttattgctga gaaaaatatt cagctgttgc ttgttcatcc tccttgagtt
    29221 gttcaatcaa ttcctctaca ctagaaaatt tcacttgatc tcttaaaaat tgattccagc
    29281 gtaccgcgac atgttcacca tagatatctt gattaaagtc caaaatatac aactctacag
    29341 tcagctcccg tccttcaccg aatgtatcat tatgtccaat tgaacccatg gctggatacc
    29401 atgtttcccc aatttttatt tccgacacat aaacaccttc tttaggcaaa tgaaccgtgc
    29461 tctttacttt gacatttgct gtagggaatc ctagtagtct gcctcgtgca tcgccatgga
    29521 caactacccc ttctacctca tatgtgcaac ctaatagttt tgctgcttct tttacttgtc
    29581 cgctgtctag taatccacga atacgggtag agctgatttt ttcaccttgt tcttcttctt
    29641 tagaaactgt gacgattttg aatcttcctt tggcatattc aggaaaatga gcgacatctg
    29701 caatgtcttt tggtccatac gtataatcaa aaccggaaac agcatatttt gcatgtaacc
    29761 ctacgatgta ttgatcaaca aattcctgcg gtgctaaatg agcaaaagaa gaagtaaaat
    29821 caacttcata taaataatct atacccaaag cggccatttt ttcttctttt tgcttaaggc
    29881 tagtcaaata tttcacgctg ctaggatcga tctttttaaa gacaatagat ggatgctgat
    29941 tgaaagtcat caatgctaat ttcaatcctt ctttttgtgc aatttccata cctgtcttta
    30001 tgactttctg gtgtccgata tgcacgccgt cgaaaaatcc cagcactaaa acgacatcgt
    30061 cttctggaat ttgttctgat tggtaaggat gtctgatctt gatgatattc attcgttatt
    30121 cctcatttct caatacttta ctaggtttta atttgttttt ttctttcggg tttggctgat
    30181 aaatactgac aacttttcct ttataaaaca aagcaatttc ttctgacggc atttctggga
    30241 gcgcaaatac agtataatca agtctcatac cgttttttac tttctgccat acttcatcag
    30301 taatatctac tcgtttgaac cgttccactc ctttttctat tggcagcaaa caatcatcga
    30361 ttgtcccatt tttcacagct tgagctactt cttctaatgt cagagcatcc ttctgggaca
    30421 taccggcact tgctgttcgg gtcagatcag acatgtgcgc agggtaacca agtttcttgc
    30481 ctgtatctac agctaatgtg cggacatagg ttcctttccc gcattctact tcgaagcgcc
    30541 aagaaatggt tcctgtcact tcgtctcttg tcaattcact tgtccggata aaacggtaaa
    30601 tctgagcctt tctaacagga cgctccactg tttcattatt gcgggcatat tcatataacc
    30661 gcttaccgtt gacttttacc gctgagtaca ttggtggtat ctgggtgatc tcaccaataa
    30721 atgaagccat cacttcatct attttttttt cagataaaga catatctact ggttttgttt
    30781 ctacccgttc tccggatcga tcttccgtcg ttgtactgaa acctagagtt atctcgccta
    30841 catagatttt cccagaatct gtcaaatatt cgataacttt tgtggctttt ccaatacaaa
    30901 tgggcaacac accgtctacg tctgggtcaa gcgtaccccc atgtccgatt tttttcgtat
    30961 gtaagatttt tcttaatttg aatacgcaat catgacttgt cattccgcgt tctttccata
    31021 aaggtaataa cccgtccatg tcttttctcc atttcatttt tgatttaact gaattattat
    31081 aacacattgc atcaattact gacgaatttg acatcataat gaacggtttt tatttttcgg
    31141 caataaaaac gtaacaaaat tcatgctcac gtaaatagaa atcatgttat gcttttttaa
    31201 aggagggaat agaatggaag attttttctt tgcaccattt gattggattt atgggtggct
    31261 tacaggtaaa tagtttggtt ttcacttccc aaaaacgaaa aaaaagactg gccattcttc
    31321 cagtcttttt tccgtttttt ttatttattt tttcagtttc ttaatcaatg taaaaaattc
    31381 tattttgcgg gatcgatttt atcattttat tggtcaagat ttttccattt ccaatccaat
    31441 acttctggca gatcaatacc attttcacga atatagtttt gatgttcttg taatttctga
    31501 tccatttttt tcgcaaaatc ttctgcttga cttcctttga tttttaaagc tgcgtctttt
    31561 gccaaatgat aacgatcaag ttcatttact acacgcatgt caaatggtgt cgtgatatct
    31621 ccattttctt tgtagccatg aatcattaaa tgatggttat gacgatcaaa gaaaatcgtg
    31681 cggaccaatg tttcataacc gtggaaagca aataagatcg gtttgtcttt tgtaaaatat
    31741 tgatcaaatt cttcatcgct caacccacgc ggatcttgac ttggatgacg taatttcaat
    31801 aaatctacga cattgatgaa ccgaattttt agttttggga attcttccag cagcaaagtt
    31861 acagctgcta atgcttctaa gttagattcc gttcctgcag cagcaatgac caattcaggt
    31921 tcctcctctt ttgctgtcga agcccaatca atgattttca agccttcatt cactaagacg
    31981 gctgcttctt ctgctgagta aaattgagca cgcggatgtt tggaagagat gatcaaattg
    32041 attttttctg acgagcggaa tgctttatcc atgactgcga gcaatgtatt tgcatccgca
    32101 ggcagatatt cacggatgaa ctccgccttc ttttctgcca aatgtgtcaa tattcctgga
    32161 tcttggtggc tatatccatt gtgatcttgc tggaagactg ttgagcttgc aatgagattt
    32221 agcgaaggat aatcatttcg ccaagaaagg tcatgtgatt tccgcatcca tttgaagtgc
    32281 tgagtcaaca tagagtcaac cactcgcaag aatgattcgt aacttgcaaa aaaccatgcc
    32341 gtccagtcag aacgtaccct tctaagaacc cttcatcttg gtgttccgat aattgactgt
    32401 cgatgacgcg accagatggc gaaagccatt catcattttc tggttcgatt ttttccatcc
    32461 attggcgatt tgtcgttttg aaaacttgat tcaatcggtt tgattttgtt tcatctggtc
    32521 caaatatacg gaaattttct ggatttttct caatgatctc gcgaacgaat ttaccaagtt
    32581 cggtcatatc ttctgcttta acggagcctg gtttttcaaa ttgcaaagta tattttttcc
    32641 aatctggcat aataagcggt cttggatcga ttccaccatt ggtgatagga ttttttgcca
    32701 ttctttggtc accagtaggc gcgatttctc tgatttcttc taaaatacgt ccttgtgcat
    32761 caaataattt ttctggttca taggatttca gccaacggag taaagcatct gcatgatcca
    32821 tatgttcttg atctacagga atcggtactt ggtgtgctcg gaaggttcct tcaattggtt
    32881 ccccgtccca ttcttttgga ccggtccatc cttttggtgt gcgtacgatc aatactggcc
    32941 attttggcat cacgacatct gttgctgtct tcaggcgagc ttctttttgg atggatttaa
    33001 ttttctcgat tgcttgatcc attttttcag ccataacagg atttaatttt tctgaatcat
    33061 ttccttcaat aaagaaaggt tcccagccaa gtccgttaaa gtaatttgct aattctccat
    33121 ctgatttacg tgccaagatc gttgggttgg ctatttttgc accatttaaa tgaaggatcg
    33181 gtaaaacagc tccatctatc actggattga taaagacatt tgagaaccag cttccagcta
    33241 aaggaccggt ttctgcttca ccatcaccaa tcacgacagc agagattact tcaggattgt
    33301 ctaatacagc tccgactgca tgtgaaagag agtatccaag ctctccgcct tcatggatag
    33361 aacctggtgt ttctggtgct gcatgagaag caatgcctcc aggaaatgaa aatcgtttga
    33421 ataatttttg cattcctgtc tcatcttccg ttacttctgg atagatctct gtgtaacttc
    33481 catctaaata agcattcgat accatgactt gtccgccgtg tcctggccct tcaatataaa
    33541 acatgttcaa atcatattta ttgatgactc ggttcaaatg gacataaata aaattttggc
    33601 caggaatcgt tccccaatga ccgattggat gttttttgac atcttcgggt ttcagtgtac
    33661 gtctcagtaa ggggttatct ttaagataaa gttgcccaac agaaagataa ttagcagcac
    33721 gccaccaagc cgtcatttta tcaaagtatt ctttactaga ataatccata cataacactc
    33781 cttttcatta ttactcttat tctagcgcag atactcccaa ttgaaaccta ataagcactt
    33841 ctgttcattt gtttggattt tatatctagc caaatgaaca ggtataaaaa accacgaaat
    33901 ttttcctttc gctgacgttt tttacattga tccaaaaaac ttgtcgttat cacaatggct
    33961 gttccgcagt tcatttcgct cctcatcatg cataatttgc tgaatgacgc tgggccagtt
    34021 aacacattgc tggaaaactg gggctggatc gagcaattcc tttcctgatt gatccgtttt
    34081 atgtaaaagt ctctgttatc tttgtcaata tgtgaattgg tatcccagct actatgctaa
    34141 tttctacagg gattatccaa aatttggatc gtgaccaaat cgaagcggcg taatcggaaa
    34201 tatcaataac ttcaacgtga tttttctttt aactgtcaac ggaccgacaa actcaaactt
    34261 ttatggtgca ggttccactg atcttctagt tacttggcta tacctgactg tcactaaaat
    34321 agattacaat ctagcttccg ttatcggtat cttgatcttt atctatatgc aaaaatttat
    34381 atactactag aaaaaagaaa gtcgaatttt ttacttttct ttttctcttt tttggttagc
    34441 tgacacgccg caagactaaa gtcatggaac tcttaggtaa tacgtcttaa tgaaaacaaa
    34501 aaaatcccgc aacccctgat ttcacaggat ttgcgggagt ttttgattag tctttgttca
    34561 agtcgcgaag caattcatcg atatggttgc catagacaac tgattcatca agttcaaaga
    34621 acaattcagg tgtcttataa atacttagat tgtgccctaa ctctcgacga atcaatcctg
    34681 atgctttatt caatccatct tgtgctgttt tcttatctga tgctaagtca gataaaatgc
    34741 tgtaatagat cgttgcttgc tgtaagtccc ccgtaacttt cacatctgtg atcgttacgt
    34801 tctccacacg agggtcacgg acctttttgc gcaaaatatc attgacttct tttaagattt
    34861 cttgaccgac tcggcggtca cggtagttag ccataatgat tggcctcctt ttatcagttt
    34921 ttagtcattt ttgacttctt ccatgataaa tccttcaatt acgtcatcta ctttgatgtc
    34981 attgaatttt tcaatcattg caccacattc ataacccatt ttgacttctt tcacgtcatc
    35041 tttgaatcgt ttcagacttg caagttggcc ttcatagatt acgattccgt cacggattac
    35101 acggacaccg ctatctctac gaatatatcc ttctgttaca taggcacctg caatcgtacc
    35161 aactttagaa actttgaagg tttcacgaac gatcatttga ccggtgattt tttcttcaaa
    35221 ttcagggtct agcatccctt tcatcgcagt ttcgatttct tcgatggctt tatagatgat
    35281 acggtgaaga cgaatatcga cttcttcagt atcagcttgc acttttgcct gcggtgtagg
    35341 acgaacgttg aaaccgatga taatagcgtt gctggctgct gctaaggtta catcactttc
    35401 attgattgct ccgacagctg aatggacgat ttttacacgg acaccttcca catcaatctt
    35461 cttcaatgat gcagccagtg cttctgctga accttgtaca tctgctttga tgattacgtt
    35521 aacttctttc aattcgcctt ctttcaggct ttcaaataag ttatccaaag tcacacggct
    35581 tgtggcagca cggttttcta ataatgcacg tttcgcgcgt tcttcacccg ctgcacgtgc
    35641 tgtcttttcg tcttcgaaga ctacgaaacg gtcacctgct tgaggtacat cattcaagcc
    35701 agtgatttct actggcgttg caggtcctgc ttctttatcg cgacggccaa gatcgtttgt
    35761 cattacacgg acacgtccgt aagtattacc gacaacgatt gggtcaccaa catgaagtgt
    35821 tccttcttgg acaagtaagg ttgacacagg acctttacct ttatcaagac gtgcttcgat
    35881 cactgttccg attgcgcgtt gtgttggatc tgcttttaaa tcttctactt ctgcgactaa
    35941 caggatcatt tccagcaatt cttcgatgtt ttggccgaat ttcgctgaga tctcaacaaa
    36001 gatcgtttct ccaccccatg cttcaggaat taactcatac tcacttaatt cttgcataac
    36061 atgttgaggg tttgcacctg gtttatcaat tttatttaca gcgacgatga ttggaacgcc
    36121 tgcagctttg gcatggttga ttgcttcaac tgtttgtggc atgacgccat catctgcagc
    36181 aacaactaag atcgtgatat ctgtgatact tgctccacgt gcacgcatac ttgtaaaggc
    36241 cgcatgtcct ggtgtatcca agaaagtgat tggtttacca tcaatatcga tctggtaggc
    36301 accgatatgc tgtgtgattc cgccggcttc gccgcttgtt acacgtgaat ggcgtaatgt
    36361 atccaataaa gttgttttcc catggtctac gtgtcccata atcgttacga ctggcggacg
    36421 tgagactaat ttatctggat cgacttcttc tgcctcgaag aatttatcga tatcagcaat
    36481 gtcaacttgg actttttctt gtggttccat gccatagtcc gtcgctaaca gttcaatcgt
    36541 atctttatcc agtgcttggt tttgattgac catgacacca agcataaata attttttaat
    36601 gatttctgct ggctcacgat gaattttttt cgcaatatct gctacgttca taccttcagt
    36661 atactctaat acatctggca attcacggaa tttgcgtggt ggtacagctg gtttagttga
    36721 ttgttgctgt ttgccttttt tacctttttt attataacga ttacggttat tattattaaa
    36781 tttgccgcga ttgttattgt ttggtttgtt gttattgtta gtacggtttt gattgccgcc
    36841 ttggctcgtt ccttgacgat tttggctgct ggtatttccc gtattatttt ggtttgttgt
    36901 tggttttttt ccttgattca cttgaccgct cccttgaccg cgatcttgat aactgcggtt
    36961 atttttattt ttttgttgat tgttcgttgt ttctttttta cgttgatcgt tcggctgttg
    37021 attggctggt tttctgcttg cttgttgtgc agcttgttgt ttcgtgcttg ggtttttgaa
    37081 cgcttgttgc aatttcttct catcattgct agtaatcgca cccatatggt ttttcacatc
    37141 gatgccaagt tgttgtgctt tttccacgac gtctttacta gattttttga gttcttttgc
    37201 taattcatag attcttttgt tgcccatgca atcaccttcc taaccttcaa ttagctcctt
    37261 ggtcttcttc gcaaatccgg catccattat tccgaatacc ttgcgaggtt ttccgatcat
    37321 ctgcgtgatt tctgcttcag aaaatagctc aaagcaagga acttcataat atgaactctt
    37381 atcttttatc ttttttcttg tattctcgct tgcatcggtt gccacaaaca ccaattttgc
    37441 tttttgtttg cggatttcag caatggtcag ttcttcaccc gtgattagtt ttcccgcgcg
    37501 catcgctaga ccgatgagat tcatggcttt ttgccgatta actgtattca ttttccaaat
    37561 aactctttac gtgctttttg gtgagtgaca tagtcaagaa gttcttgata aaactcatct
    37621 gttaatgttg tttcgagtac tcgatcgaga atttttttat cccaagcttg ttgtacttct
    37681 tccggctcaa gtgagacata cgctcctcgt ccaggcattt ttccagttgg atcgatcgat
    37741 acttcccctt cttttgaacg ggtgattcgg accatctctt ttttaggttt catttcacct
    37801 gaaaccacgg atttgcgtaa aggaattttc ctttgtttca tctctttgcc tccttactaa
    37861 agttaagctt ctggtttttc ttcgaccgtt tcattgttga atgcaattgt ttcatattca
    37921 tcgtctgtta aatctgactg aacgattgct tcatcatgca gttcttctgt ttctttttgt
    37981 tgttcttgat tttcataaaa ttccgtcatg tcagattctg atttgatatc gattttatgg
    38041 ttagtcaatt tagcagctaa acgtgcgttt tgtccgcgtt taccgattgc tagcgataat
    38101 tggtagtctg gaacgacaac tgtgcaggcc tttggatttg tctcatcgaa gatcacatcc
    38161 atgacttgtg caggattcaa tgcatttgca atgaatacag ctggatcttc attccattcg
    38221 acgatatcca tgttttcgcc ttttaattca ttgacgattg cttgaactcg ttgtcccttt
    38281 ggcccgacac atgtaccgac tgcatcgata tttggatcat ttgagcgaac tgccactttt
    38341 gaacgatcgc cagcttcacg ggcgatgctg acaatttcaa cgattccatc atagacttct
    38401 ggtacttctt gctcaaataa gcggcgcaat aggtctggat ggctgcggct aacaaatact
    38461 tgcggacctt tagacgtatt ttctactcgg gaaacataaa ctttgattcg atcatgtggt
    38521 tgataaaatt catttggcat ttgatcttgt ttagaaagaa ctgcctcaat tttaccaaga
    38581 ttgacataaa tataacgttt atcctggcgt tcgacgattc cttgcatgat gtctttttca
    38641 taagcactga attcgttata aataatcgtt ctttctgctt cacgtacacg ctgtaaaata
    38701 acttgtttag ccgtttgcgc agcgatccgc ccaaaatctt ttggtgttac ttcaaaacgg
    38761 atcgtatcac cgatctcata tgctggattg attaaaagtg catctttcaa tgagacttcc
    38821 aactgtgaat ccatcacttc ttccgttact tcttttaccg catagacatg gatctttcct
    38881 tttttctgat caaattccac ttcgacgttt tgtgcttggc catagtgacg tttgtatgca
    38941 gaaaccaatg ctgcttctaa agcatcgatc acgatttctt ttgagatccc tttttcagct
    39001 tctaaagcgt ctaacgcatt taacatttct ttactcattt tttctgattc ctctccgtga
    39061 ttaaaattgg atagctaagc gagcttttgc aatatttttt cggtcaaaag tcaattcttt
    39121 ttctcgtgtt tttatgcgta ttttcaatgt aagctgttca gcatcaaatg attgaagaaa
    39181 tccttcatat tgtttttcgc catctactgg ttggtataag gagacatgga cataatcacc
    39241 aagtgccttt tcatagtcgc tttctttttt caatggccgc tctgctcccg gggaggatac
    39301 ctccaggaaa taagcttgag gaatcggatc tggatctgct gcatcaagtt tttcactcag
    39361 tttctcactg acaaaggcgc attcttcgat atcgataccg ccttctttgt caataaatac
    39421 tcgtaagaac cagctttttc cttcttttac aaattcgact tcgacaagtt caaaattttg
    39481 ttcatctaaa atcggtgtca ccatttctgt gactgtttca acgacgctac tcaaaagttt
    39541 cgcctcctta aaaaattaag tgcgtaattc cattaaaaag agtgagcgaa aaatccgctc
    39601 actcacagtt agtatcatta cctataagaa ctataccata ctctgcttaa aattacaagc
    39661 agacaggaaa acagatgctt atagcatatc gaataacgat aattgatttt catctggtag
    39721 atctttcaat acgccattgt cgttcatata ttcgatcaat gtttttgaaa ctttaccgcg
    39781 ggtggcaaga tcttctttgg ataaaaatgg accgtctttt cttgcttcaa cgatttgttt
    39841 tgccacgttg gttcccaaac ttgggacagc tctaaatggt gcgatcaacg tatcaccttc
    39901 aataacaaag ttaacggcat ccgatttata caaatcaatc attccaaatt tgaatcctcg
    39961 ttccagcatt tcattggcta actccagcac tgtcagttga ttcttttctt ttacggaagc
    40021 atctaatcct ttgtccgtga tttctttcat tgcttgtttc acagcatctt ttcctttgca
    40081 catggagact agatcaaagt cgtctgcacg tacagaaaag tatgcacagt aatacaaaat
    40141 agggaaataa accttgaaat aagcgacacg aagagccatc aatacgtaag ctgcagcatg
    40201 ggctttcgga aacatatatt tgatttttga acaagaatcg atataccagt cgggcacatt
    40261 attttctttc atcgccgata gataggtttc tcgcaattca tcagggatct tgttccattg
    40321 tcctttacga acagtttcca tgattttaaa tgccatcccg ctatctaatc cagcatggat
    40381 caaataaacc ataatatcat cacgacagcc gattacttcc gccagtgtcg catctcctcg
    40441 tcgaatcaat tcttccgcat ttcctaacca tacgtctgta ccatgagata gtccggaaat
    40501 ttgcagtagt tctgcaaaag ttgtaggatg cgtttcttct agcatccctc gaacaaaacg
    40561 agtgccgaat tctggaatac ctaatgtccc tgttttggaa taaatttgtt cctgacttac
    40621 tccaagtact tcaggaccgg caaatatccg catcacttcc gggtcatccg tagggatcgt
    40681 ttgtggatcg atccctgata aatcttgaag catccgaata acagttgggt catcatgccc
    40741 tagaatatct aatttcaata cattatcgtg aatcgagtga aaatcaaaat gagtcgtttt
    40801 ccattccgaa ttttgatcgt ctgctggata ttggatcggc gtaaaatcat agacatccat
    40861 ataatcggga ataacgatga tcccccctgg atgctggccg gttgtccgtt tgactccagt
    40921 agcgcctttt gctaatctgt cgacttctgc acttcgaaac tgcagattat tgtccctttc
    40981 gtatcctttt acatagccat atgctgtttt gtcggcgact gtaccaatcg ttccggcacg
    41041 gtaaacatag tcttcgccaa acaatacttt cgtatagtta tgggcttctg cttgataatc
    41101 acctgagaag ttcaaatcaa tatcgggcac tttgtctcca tggaatccta aaaatgtctc
    41161 aaatggaata tcatgaccgt ctttgtttaa gcgtgcacca cattttggac attgtttttc
    41221 tggcatgtca aatccagaac catacgtacc atcttcaaaa aactcggaat attggcactc
    41281 tggacaataa taatgagggg ctaacggatt aacttcagtg attcctgtca tcgtcgcaac
    41341 aaagctagag ccaacagagc cacgtgaacc tactagatag ccatcttcat tacttttatg
    41401 cactagtttt tgggcgatca gataaataac cgagaagcca ttaccattaa tagaattcaa
    41461 ctcttttttt aatcgttttt gaatgatctc aggtaacgga tctccgtaca tttgttttgc
    41521 ttttgtgtaa ctcaattctg atatttcatc ttctgaacct ggaatcttcg gtgtataaag
    41581 ttcatctttg actggaatca cttcttcgca gatatctgcg attttattcg tattttctac
    41641 tacgatctct ttcgctgttt cttcccctaa aaagtgaaat gcagttagca tctcatcggt
    41701 tgttcggaaa tggacatccg gcaaactatg acgattcaac ggattcgctc cgcccataga
    41761 attgatcaaa atttttcggt aaatggcatc ttcttcgttc agataatgta cattacctgt
    41821 tgcaacaacg attttatcca aactttttcc gatttctacc aaattttgga taatttcttc
    41881 caaatcatgt tcatttttta ccaattcttg ctcgatcaaa ggcgcataga ccgcttttgg
    41941 cataacttca atataatcgt agaattttgc tcggtttctt gcctcttcta cccctttttg
    42001 catcatggct tcgaatattt ctcccttatc acaagctgag ccgatcaata gattttcacg
    42061 catctttttc aattgggagc gtggtattcg tgggacgcgt tcgaagtatt cgacattcga
    42121 cattgaaatc agtttgaaca aatctttcag tcctgcctga ttttttgcta aaatcgttac
    42181 atggaaaggt cgagcccttt tgtaagaatc tccttctccg atatgctcat ttagctgatc
    42241 atgataaagc atgttatgat tgtccattgc ctctttcaca aagatccatg ctaagtgacc
    42301 agttgcttca gcatcataga ttgctcggtg atgctgttcc aagctgacac caaatttttt
    42361 agataacaca cctaatccga aacgtttgaa ttgaggatac agatatctag ctaattccaa
    42421 cgtatcaatg actggattgg ccgcttcagg aataccatac cttgcgtagc tagtattcaa
    42481 gaaccccata tcaaaagccg cgttgtgggc taccaaaatt gtatcttttg aaaattcaag
    42541 gaacatccgc aatacttctt cttccgactt tgatcctcga accattccat ccgtgatacc
    42601 agtcagatct accgttgttc gtgacaaagg atgtcctgga tcgataaatt catcaaagct
    42661 ttcgatcaca tttcctttat acattttgac tgcggctagc tcgatgatcg tgtcatatac
    42721 tgcagacaat cctgtcgttt ccacgtcaaa tacgacatat gtggcttcac tcaatgcttc
    42781 atgtgcatca ttataagcaa ttgggacacc atcatctacg acattcgctt ccacgccgta
    42841 taagattttc acacctgctt tttttccagc actatgtgct tccggaaagg cttgcgcacc
    42901 accgtggtct gtaatggcga tagctcggtg tccccatttc cctgcttgtg ccacaagatc
    42961 agagatgctg tttgttgcat ccatcgtact catattgcta tgaacatgaa gctctactcg
    43021 tttttcccct tcaggagcat aatctttgcg aggtgtatgt ttgacttcta tgatatcttg
    43081 cgcattcatg accaaatcac ggacaaacgt atcttcttga atacttcctc gtacctttaa
    43141 ccagctaccg acactgatcg catcaaatac ttgttcatct ttttcgccgt tcgagaattt
    43201 tttgacgata aatgatgaag tataatctgt gattttcaaa gtcaagatct tgcgttttga
    43261 acgtagttct ctgacttctt tgtcgaatac gtacccctca agtgtgactc ttcgttcttc
    43321 ttcaacgata ctgatcattg gtgtcgttgg ttcatcggct ggaatatttc gtcctagctg
    43381 gatgtggatg ggtccttcaa gagctggcga ttgctctttt ctttcctttt tcttttgttc
    43441 gtggacaact agactttcag ctgcttgctt cataaaggct tctgcctgtt cttgtttgcg
    43501 ttcttcaaaa agtttcaata ctcgttccgc ttgctgctca tccacttctg gttcgatacg
    43561 aaattttggg aatccgtaac tgacgaacaa ctcttctact aatggcaagt attgttgctt
    43621 caaataagaa atagctcctg tactgtcgat tggcaagatg actttacgat cttttatcac
    43681 aggtacctgc gttttcaata cttgctgtgc tagtggtgta tcgcattgct gattttccaa
    43741 agcttgggcc cagtaatctt gcaagagctg ttcatcgaat gtctggtcat ctgctaagat
    43801 attcagtttg acttgtgcaa tatctttaaa agcgatagtc agatgctgag tcaatgaccg
    43861 atatagcata ataggcaaaa ttttggaaaa atgcagcgta aattcccata aacgcgattt
    43921 acgatggacc actacactat ccatttcacc agcagtaagc agtggatgat ttttttctgc
    43981 ttcctctagt tggatttgtt ccaacagttt ttcaaataaa tctcgttttt ctgacaaaga
    44041 tatacgccct ttctgatgag aaacaatgca attgatcgca tcttatcctc ttctttttca
    44101 gtacctttta gtatactcgt tttttctgag ttttacatta aaaaattgga actgcgattc
    44161 aaatcgcagt tccaatttta aaagcgaatc atcagattgt caaaaacagc tgattcagct
    44221 atttttattt tctgtgtttt aacgcttatt attcgccttg gttcaagagg attggtaaag
    44281 tattcaccaa ttcttcttta cgtacttcaa ccatttcgcc agttttcttg attttcactt
    44341 ctacgattcc atccactgct tttttaccga cagtgattct gattggacag ccaatcaaat
    44401 cagaatccgc gaatttcact cctgcgcgtt catttctgtc atcaaccagt acttcatagc
    44461 cagcttttgt catcgcttct tctagttcat tcgtcaggtt tgtttggtat tcgtctttta
    44521 gattcatttg gacgatatgc aaatcaaatg gcgcaattcc ttttggccag ttgattcctt
    44581 gttcatctga atgttgttct acgattgctg ataataaacg acttacaccg ataccataac
    44641 agcccataat cacaaatttt tctcgtccat tttcgtctag tacagttgct cccatagatt
    44701 cactgtaacg agtgccgatt ttgaagatat gtccgatttc gattcctcga gtaaaggcaa
    44761 gaacaccatt gttgtctggt gaaggatctc cttcttgtac aaaacgaaga tcttcataag
    44821 aaagtacagt gaaatcgcgt tctggattca catttttata gtggaatcct gtttcgttcg
    44881 ctccagtaat ggcattcgct aaatcttgga cataacggtc tgcatatact tttacgtcat
    44941 ctgaaacacc gactggtccg acagaaccaa actcagcacc tagatatttt tgtgcttctt
    45001 cttctgttgc ttcttctaaa aagtcagcag ataaaaagtt tttcagtttt acgtcattga
    45061 cttcatggtc tccgcgtacc aaaacaagga caggttgttc atccgcaatg aacaatacag
    45121 acttgatgat tttttgaggt tcaacagaaa agaaatctgc tacttcgtca attgatttta
    45181 cgttaggtgt agctactttc tccaattcaa gctgtgtttc atgcgatttt tttgatacat
    45241 agtagcttgt agccatctcc aagtttgcag cataatcact ttcagtcgaa taacagatcg
    45301 tatcctcccc gatttctgag atcgccatga attcttttga gtctttcccg cccattgcgc
    45361 caccgtctcc gataatcgag cgaaattcta agccacaacg cttgaatact tcggtgtagg
    45421 ctttttcgta atctttatat gtctcgtcca aactttcttc cgttgcatgg aaagaatacg
    45481 catctttcat gataaactct cttccgcgaa gcaaaccgaa gcgaggtcgt ttttcgtcac
    45541 gatatttagg ttggatttga taaagattta aaggtaattt cttatatgaa ttgatttcat
    45601 tacgcatcaa atcagtaaaa gtttcttcgt gagttggtcc aaggatcatt tttcgatcat
    45661 tgcggtcttt gaaacgatac aagtttggtc catatgtttc gtaacgcccg gattcttccc
    45721 aaagttctgc tggcaagatg gcaggcatca gcatttctac cgctccgatt ttttcaaatt
    45781 cttcccgcat aatgcgtttc agtttttcca atacacgatt tgctaatggc agatatgaat
    45841 aaatacccgc tgatacttgg cggatatatc cagctcttag caagatttga tggcttaata
    45901 cttctgcatc atttggaact tcgcgtaatg ttggaatcaa catttttgat tgtctcattc
    45961 tatatactcc tttatttcct caaaatagtt atcgtttgtt attataccct atttgtcaac
    46021 tttttctaga aaaagaatcg ttgaatatcg ttccatgtaa ctagcaccat caacaacatt
    46081 aaaaatccaa agccagctaa tgtcaatatt ccttcttttt cttgacttaa tggtttcccg
    46141 cgaatccctt caaaaatatt caagactaat ttcccgccat ctaaggcagg aatcggaagc
    46201 agattgacga ttccgagatt cattgaaaga agcgccatca aactaataac agttgttaca
    46261 ccttgatttg ctgcttctga agacaattgg tacatcatga caggtccgcc taatttatct
    46321 aaactgaaac ctgtaaatag tgatcctaag gctttgaaaa tttccaatga accgctgaaa
    46381 gcttgacgcg tccctccaat gatcttatcc ataaaacctg tattcattgg ggcttttata
    46441 ccaagttggc caactttttc gccatttgac tctacagatt tcggtgtcac gtcgacagat
    46501 tgcacctgac cttctcgttc gatcttaaag tctaatgttt tatccggatt tttcgtaatg
    46561 actgttgtca gatcattcca tgaatggatt tcttttccgt ccacactgac tacctcatca
    46621 ttttctttta agcctgcttc tgccgcagct ccattaggca tgatctctcc tacgcgatta
    46681 gtgttcgtca cttgaacacc accttgcata aatgccagaa tgatgaataa caggattgcc
    46741 aaaataaagt tattcatggg tccagcaaag ttggtcagca tacgctgcca caatttagcc
    46801 gattggaact gtacgtcttt tggcgcaatt ctgatttctg ttccgtcgtt ttcaataatc
    46861 gtcgcatcgt gatcaacagg gtaggttact acttctgttt cgtctccatt cacataacca
    46921 gtgatagtca gctcatcttc taaatcataa cggctcaatt ccatcgggat agcatttgtc
    46981 aattggattt tcttacttaa atttatcttc tctacgatgc cgtcagaatt tagtaaaaga
    47041 gaaagaggca ttcctggtgc catttctgtt tcatcatccc cattgcctgc cattcgaaca
    47101 taaccgccaa taggtaatag gcgaagcgta tatgttgtcc cgtcttttgc ttgatgccca
    47161 taaattttcg gacccatacc gatagcaaat tcgcgaacga ggattcctga tcgtttcgca
    47221 aagaagaaat gaccaaactc atgaacaatc actaatatac caaaaacgat gataaatgtc
    47281 agaatcgttt tcatagcttt tttctccttt acttgttttt tgtgtttttt ataaaacaac
    47341 gataaaacac tataatttta ccatagtata ccaaagacac caatagtatc aatataatga
    47401 aaaataaaaa cctgcgtctt ttatgaaatt cacaaagaaa cgcaggtata cttactaaat
    47461 aatgccaaat aaatgcatga taggaaatac aaacagcaaa ctgtcgaaac ggtctaagat
    47521 tccaccatgg cctggtaaga tatttcccga gtctttcaca tcataatgac gtttgattgc
    47581 agattcgacc aaatcaccaa attgtccgac tgctgaaaaa ataatcgtca tcagaagcat
    47641 gacaaacata ccatgattaa atagttcttt tgctgggtat agcaataaat aaagaaatgc
    47701 gactacaaca gcactaccaa taccaccgag cgcaccttca atcgttttgt tgggcgaaat
    47761 atcaggactt aacttatgct gaccataacg tcgaccaacc atatatgctc cgatatcagt
    47821 tgcccaaaca ataaatagac caaatatcaa aacagccaag ctttctgttc gtgcattgac
    47881 aaaattctgg aaacctattc ccacatacaa gctgacaagc acaggaaaac ctgcttcatc
    47941 aatcgtatac gtatttttag aaataactga tgttcctaaa agaatcataa ctataagata
    48001 aaataacata aatgtactta cattctctgg aagaaagaaa aaccatcttt cttttggcaa
    48061 aacaagtaac actgcaccaa cggcggataa aactccttca aaacttaaga gtgtcaatcc
    48121 tttcatccga aacagctcgt aaactcctat tactgctaag attgctgctg taagctcaac
    48181 cgctatgcct ccgatccata taatcggaat aaacaagact agcgccacgg ctgctgtgac
    48241 cacacgttgt ttcatgactg atcccctttc tcatcttctg ttttttttac accgccgaat
    48301 cgacggtctc gattttgata agaggcgatt gcttcttcaa gatgcgcacc atcaaaatca
    48361 ggccaaagcg cttttgtaaa atacagctca ctatatgcga tctgccataa cagaaaatta
    48421 ctaattcgct cttctccact tgtacggatc atcagctcag gatcttgtaa ctctttaggc
    48481 aagaaacctg tcattaaatg cttagcaatc aattcatcat cgatttcttc tgcgtgtatt
    48541 tctccttttg ctacttcttc ggcaatttct tggacagcag tcacgatctc tgctctgctt
    48601 ccataattca aagcaaaatt caataccatt ccagtattgt tttttgtttg ctcaattgca
    48661 cgtcttacag catcttgcgt atgttctggc aatacgtttt cataacccat gacatgaact
    48721 ttgacatttt ctttaatcaa ttctggtacg aacgtatcaa agaagtcgac aggtagttgc
    48781 atgagaaaac ttacttcatc ttttggtcgc ttccagtttt cagtcgaaaa ggcataaagc
    48841 gtcaacactt tcactccaag ccttgatgct ttttttgtga ctttctttac tgtttccatg
    48901 ccttctttat ggccagcaac tcgtggcaaa cgacggtttt gcgcccaacg accattgccg
    48961 tccatgatga tggcaatgtg ctgaggaatt ggtccatcgg tattaaattg aaattcgctt
    49021 ttttcttgaa tatatttgtt tttttgcgga aaaaaacgta acattctttt tcctcctata
    49081 cttaaaagat tcccactatc ttaagtatta tagcaatatc taactgtttt tcctatgaaa
    49141 tcattgtttt tttatttaaa aactgaataa ttttttcttt ttttaatttt taaataaaag
    49201 aaatataaat aaaaacagag gaagcggaat gatccgcccc tctgttttta tgattttatt
    49261 aaacttccaa taattctttt tctttatcag cagcgatctt atctacttct ttgatgctgt
    49321 tatctgtcaa tgtttgtacg tctttttcta atccgcgcaa atcatcttcg gtaatatcac
    49381 catttttttg ttgtttttta tattcatcga tcgcatcacg acggatgtta cgaatcgcga
    49441 tttttgcatt ttctgcttct tttttaacat ctttagctaa ttcttttctg cgttcttctg
    49501 tcagttgcgg gataactaag cgaatgacat ttccatcatt ggttggactg attccgatat
    49561 cacttgccat aatagctttt tcaacatctt gaagaatgct cttgtcaaaa ggtgtgatca
    49621 ttaaaacacg tgcttctgga atttggattg atgccatttg gttcaatggt gttggcgcac
    49681 cgtagtagtt cacagtaata cggtccaaaa ggctagcgtt cgcacggcct gcgcggattt
    49741 gcccaagttc acgttgaaga ttttgtgctg cttttttcat tttttctttt gcttctgtca
    49801 taattgcatc tgccattttt atttccccct tacagttgtt ccgatatttt cgcctaagat
    49861 tgcacggcga atatttccgt gttcatttaa attgaagaca agcagtggaa tatcattatc
    49921 catgcttagt gaacttgcag ttgaatccat gacttgtaat cctttggcaa taacttccag
    49981 atgagtcagt tcttcaaatt ttactgcatt tgcatctact ttcggatcag cagaataaac
    50041 accgtctaca ttgtttttag ccatcaggat aacatctgcg ccgatttctg ccgcgcgtaa
    50101 tgctgctgtt gtgtctgtcg agaagtacgg gttgccggta ccgcctgcaa agataacgat
    50161 ccgtcctttt tctaaatgac gttctgcttt acggcgaata tatggctctg cgatttgacg
    50221 catttcaatc gaagtttgca cgcgtgtcgg tacacccacg ttttccaacg tatcctgtaa
    50281 agctaaggca ttcataactg tagctaacat acccatgtaa tcagcttgag cacgttccat
    50341 gcccatttgt gctccgattt gtccgcgcca gatatttccg ccgcccacaa cgattgccat
    50401 ttcaacgccc agctcgtgaa cttctttgat ttcttggatg atttctttga ttactggtgg
    50461 tttgattcca aatcctgcgt ccccagctaa agcttctcca ctcaatttta agactacacg
    50521 ctgatatttc ggtgtgacca tgttcattcc tccaatattt tctattgcca ttttaccata
    50581 cttagatgat ttcgacactc taagactggt ttttcttgtt ttgcttaaaa aaagggagcg
    50641 cacaaaccgt gcgttcccgt ctgttattca acagaattat tttttcactt ggctcataac
    50701 ttcttctaca aagttatctt cgcgtttttc gataccttcg ccaacttcga aacgtacgaa
    50761 tgttttaaca gttgcgcctt ttgatgcaac gtatttttca acagtcatat caggatcttt
    50821 aacgaatggt tggtccacca aagcgatttc tgctttgaat ttcttcaagc gtccttctac
    50881 cattttttca acgatattcg ctggtttgcc ttcgttcaat gcttgttctg taagaacagt
    50941 tttttcatgt tctaattcag cttctggaat ttgtgattcg tttacataac gaggattgat
    51001 agctgctacg tgcatagcta cgtctcttgc aactgtttcg tcagttgttc catctagaac
    51061 agtcaatacg gcaatacgtc cgcccatatg caagtatcca ccaaatgcag cattgtcgtc
    51121 tttttctaca acttcaaagc gacggaagct gattttttct ccgataactt gagttgcttc
    51181 gatcaagtca gattcaattg tacctttatc tgttttgatt ttcattgcag cttccatgtc
    51241 tgctggtttg ttttcagcaa caagttctgc aatttctttt actaaatctt ggaacatttc
    51301 gtttttagaa acaaagtctg tttctgagtt tacttcaacg attgctgcag tgttaccttt
    51361 cacagcaact gaagccaatc cttcagctgc aatgcggtca tttttcttcg cagcctttgc
    51421 cattcctttt tcacgtaaaa gatcaactgc tttttccatg tcgccttcta cttcaactaa
    51481 tgcttttttc gcatccatca ttcctacgcc agtcatttcg cgtagttctt ttaccatttt
    51541 agctgtaacg tctgccatgt ttaactcctc ctgtgtgttt ttctttttaa aaaaagctgc
    51601 ttcaaaggag agtgaggctt gtcgcccagc ctttgaaaca gctccatcat tcaaaattat
    51661 tctgctgaag aattgtcgcc ttcaacaaca tctacgattt cttcgattga tgttgctgtg
    51721 ttttcttctg tgaaatcttc ttcaactact tgatcttcac cttggtttcc ttcgatgaaa
    51781 gcatcagcca tttttgaagt aatcaattta acggcacgaa ttgcatcgtc gtttgaaggg
    51841 attactacgt cgatttcatc tggatcgcag tttgtatcaa ccatagctac gattgggata
    51901 tttaatttgt gtgcttcttg aacagcaata cgttctttac gtgggtcaac gatgtacatt
    51961 acatctggga ttcttggcat atcagcgata ccgcctaaga atttttcaag acgttcacgt
    52021 tctttgttca aaccagcaac ttctttttta ggtagaactt caaaagttcc atcttcttcc
    52081 attgcattga tttgtttcaa acggctgata cgtttttgga tagtatccca gtttgtcaat
    52141 gttccgccta accaacgatg gttaacgaag tattgtcctg cacgagttgc ttcttctttg
    52201 attgcttctt gtgcttgttt ttttgtacct acgaataatg ctacgccgcc ttcttcagca
    52261 acgctcttca tgtaatcata agctgcatct actaatttaa ctgttttttg caagtcaatg
    52321 atgtagattc cgtttctttc tgtgaagata tatttcttca tttttggatt ccagcgacga
    52381 gtttggtgac caaagtgtac gccggcttct agtaattgtt tcatagaaat tactgccatg
    52441 tttgtttcct ccgatttggt tttatttttc ctcttcttat cttctacctt gccggcaaac
    52501 tttttcaagc acctaccgac aatccaataa gaatgtggat ttgctgatgc ataaagcacc
    52561 ctcagctatt atacagacaa aagatccgta tcgcaagagc aaatatactt ttctgctcaa
    52621 cattcaatag tttacctgaa agataaaaag aattcaagaa aaacagtaga tttctctttg
    52681 tttctttatt atcctttttc cataaaataa ttttaggtat aaaagacgaa tattattggg
    52741 caatccttct tttttttgtt atgatacatg tgaaatcttt cgtaaaggag aaagaaaatg
    52801 cgcaaagctc aaagacattt gctgattaaa caagtcattg aagaattccc gatccgtacg
    52861 caagaagaac tattaaacaa acttgggcat tatggcgtta ccgccacgca ggcaacgatt
    52921 tctcgagata tccgtgatct aaaaatcgtg aaggcacctg atgaaaatgg cgtttcccgc
    52981 ttcgtattgt ttcgcgaaaa aggaactcct gagactagga ctgaggaaga ggatcggctg
    53041 gttcaaatga ttgaagacat tgtattgaaa gtcgaacgag tccacttttt gacgatcgtc
    53101 aataccttgc cagacaatgc tcatttattt gcatctgttt tagatgagat caaacctccc
    53161 tctattgtca gtacaattgc tggattcgat acgatcatgg tcatctcgga aaatgaggag
    53221 gacgcaaaac gagtagaaga atttcttcac catccaactg aagtatcact tttttaatca
    53281 gagcacgtat tcctaatatg gaatgcgtgt ttttttattt tcaatatttt ccttattgaa
    53341 taaaccttta cactctttac aaaaatacat cattttttat tcaaattttt caacaccttg
    53401 attgaatcac aataatcatc atttatattg gatgttaaca agagcagctt cttgctcttt
    53461 gtgtatttca aaaaagaaat gaaaggaaat catatgtata aattattaac ttctggacca
    53521 ttgacgactt ctgaaacagt gaaaaatgct atgctgatcg atcattgtac ttgggatcaa
    53581 gaatacaaag aaattacaca atggataaga aaaaaattat tggatcttgc tcaagtatcg
    53641 gaaggaatat acacaacggt cttaatgcaa ggtagcagct ccttcggggt agaatcggta
    53701 ttaagttctg tgatcaggcc agaagatact gtactgattt gttcaaacgg cgcatataga
    53761 caacgaatga tcaacatgtg cgaatgtctg aaactgaact ataccgttta tgaggtatcg
    53821 gaacatcaaa tcctccaagc tcatgtgatt acacagctga ttgaagagaa tccgtctatc
    53881 tccgcactag caatggtcca tagcgagact acttccggta ttttgaataa tttggaagag
    53941 attgctgctg tcacacaaga aagagatctt ttgtttatag tggacgcgat gtcaagcttt
    54001 ggtgggatac cgatttgtgt agccgaacta gggattgatt ttttgatttc cagctcaaac
    54061 aaatgtattc aaggtgttcc aggcttttct tttgtacttg ctaataaagc ggaactggaa
    54121 aaacgaaaaa atgttagccg aagtttgtca cttgatctat atgaccagta ccgcgtgatg
    54181 gaaaacgaga atggcaaatg gcgtttcact tcccctaccc atacggtttt ggcttttcaa
    54241 caagcgctga aggaactaga ggcagaagga ggaatcgaaa ctcgatattc acgttatctc
    54301 gccaataatc atgtgctacg acaaaaaatg acagagatgg ggttccagcc tttcattcaa
    54361 gaatatcagg ggccattcat cacctctttt ctttacccta aggactggcg atttgatttt
    54421 aatcattttt atcagtattt aaagcaaaat ggatttgtga tctatcccgg gaaaatcagc
    54481 gatcatgaat gttttcgtat cggaaatatt ggcgagatct atccggaaga tattttggaa
    54541 ctgacagcaa ttatcaaaga ctataaggag aacgttaatg attaaaactg taatttttga
    54601 ttgggcagga acaactgtgg actttggctg tatggctcct gtccacgctt ttagaaatgc
    54661 ttttctagaa aaaggaatcc aactgactga caaagaaatc cgctaaccga tgggaaaatt
    54721 aaaatgggat catatccaac agcttctttc cctcccttct gtcaaaaaac aatggatagc
    54781 tatttatgga caactacctc aggaaaaaga ggtggaggaa ttgtatcaac aatttgaaca
    54841 ttatctgttt aaagatttgg tcaagcattc taccttgaaa cccgacacat tggaaacgat
    54901 cgaacggttg agaaacgctg ggatcaatat tggttccacg aatggattta atgctcctat
    54961 gatgacaatc gtagcagaaa ccgcaaaaaa agaaaggtat tcaccagact tcatggcaac
    55021 ttttgaagac gtcgaagggt atggtcgacc gtttccttat atgatccata aaaacatgca
    55081 gtacttcaaa aacaagtcgg ttgatgaagt aatcaaagtc ggagatacgg tctcagatat
    55141 ccaggaaggg aaaaatgccg gagtgttgac agtaggtgta attgaaggta gttcgctaat
    55201 ggggatttct tatgatgagt accaaaaatt agagatgggg aaaaagatcg tttaaaacag
    55261 tctgttgctc ttagattcca acaagccggc gctgatattt gtgtaaacaa tttaaaagag
    55321 ttaacagaat acattttgta cgctgacatg aatccagaat gacttttata caaaataaga
    55381 ggttgtgaaa aagctgtcct gtatcaagta ataaaaacag ccaagcaaaa atagctcctc
    55441 atatttttcg tttggttagg cttattaccg cagatgcaag cttttgaaca ccgtttattc
    55501 gaaaaaaaaa agggagctgt gacacgactt ttgtcacagt ccccttattt gcaaacaaac
    55561 agtgtttaag agacaacctc tccactaaac ggtgttcatt cagctgactc tcggtatttg
    55621 ctcaaaaaac gataaaattt gaaaagctat tttctctttt gtttctctaa tactcggatc
    55681 agaacgctga tttcacattc tctttttctt acatcttatc tggtgcatga agtcctagca
    55741 aacgaaggtc ttctttcaat aatacagtca ctgcataaac tagagctaag cgtgcttctt
    55801 tttgttcatc atcagctaaa atcttcgtat gagcatagta tttgttgaat gcttgtgcta
    55861 atttgattgc atgtttcgca atcactgacg gttcgtattt ttccccagca gacaagaccg
    55921 tttcagggta tttttgaact aatttaacca cttcccagct ggtatcgtca ttcaatgcat
    55981 agtctgctgt ttctgaaggg acaaatcctg ctttttccaa caggctgact gcacgagcat
    56041 gtgtatattg cacataaggt ccagtttctc cttcaaatcg aacaacttct tccaggttaa
    56101 agtcaaatgt attcagtcga tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa
    56161 cttgtttagc tacagcatct ttgttttcca aatcagggtt tttctcgctg atttgttctt
    56221 tagcagattg gattgcttca tttaatactt cttctaataa gacgatcttt cctttacgag
    56281 ttgataattt tttaccacct tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag
    56341 accagtcaaa gcccatttcc ttcaagacag ctttcaactg tttaaaatga tagctttgtt
    56401 cattccctac tacatataga gattgtttga agtcgtaagt acgtttacgg tacaatgcag
    56461 cagctaagtc acgtgtaatg tatagtgtcg caccgtctga cttcttaatc aacgctggat
    56521 tcaggtcata agcagatagg tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt
    56581 tttcttctaa aagtttgacg atttcatcca tcttgtcatt gtagaaagct tcaccgttca
    56641 acgaatcgaa acgtacttcc agcagatcat agattttatt gaattctttc atcgattcat
    56701 cacggaacca ttgccataat tgaatagcct ctttatcgcc ttcttccaga cgtttgaacc
    56761 aagcacgagc ttcttcattt aattctggtt ctgtttcagc tacttcatgg aattggacgt
    56821 ataaacgaag caattcattg attggttctg ctttaacagc ttcttctgta ccccattttt
    56881 tgtaagcgac gatcagtttt ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga
    56941 ttggctgata accgattttt tccataatga aaccgatcga attaccgata actgtcgaac
    57001 gcaaatgtcc cattgagatt ggtttggcaa tattaggaga agacatgtcg attggtactg
    57061 ttccttgatt cccgatgttg ctatccccat aatgttcttt ttctttcact actgtttgca
    57121 aaactttttt gctgataagt tctttattca tgaaaaagtt caagtaaggt ccgacaactt
    57181 cgattttttc aaagttggca gaatcgattt tttctgctaa atctgcagcg atttgctgtg
    57241 gcgcttttcg gtaaactttt gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag
    57301 ctgattttgg attttctagt aattgctcga cttgttccag tgttaaatca tctttgacag
    57361 cgtcatagac agcttttgcg acaagatctt tattattcat ctttattcct cctaaagtgt
    57421 tcccttgatt gttaaacaaa aaaaatctct agcgacggca tacttagtat gccatcacta
    57481 gagacgagat ttcccgcggt accactccaa ttgtcctaaa aaaggaccag ctccatctga
    57541 tatcggtaga cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc
    57601 ttcattcggc ttccaccact tccgaattcg ctagccaaga atgatacaag tactctcttg
    57661 ttcaacaaat cttagtctta ttaaactttc ttgaattata tcagaaaaaa aaacgaaagg
    57721 ctagtcgttt cataaaaaaa caatccagat ggcctaaagg agaagaaata ccacctggat
    57781 agctataaat catttcgtaa atacgaaaga cttaacaaat taatcagcta cgataatcgt
    57841 tccttcttca gaagctaaaa gattctcgat attttctaaa gaagtgatga ttgccttagc
    57901 gtttggtttt gcttcaacaa atgcaatagc tgcttctact ttcggaagca tgcttcctgg
    57961 agcgaattgc ttttcatcaa tgtattgttt catttctgaa acagtaacag tttctaattt
    58021 cttttgatct ggtttttggt aattgacata cacattgtct actcctgtca atacgatcag
    58081 caaatccgca tcgatgattt cggctaattt ttctgaagca aaatctttat cgattacggc
    58141 ttctcgtcct tctaatccag tagctgtttc tacaacagga atcccaccgc caccaacaga
    58201 gactgtgata actccttgat cgaccagcgt ttcgatgaca cgtgcttctt taatcgagat
    58261 gggtttaggg gaagcgacta cttttctcca tccacgtcca gcatcttctt tgaatgtcac
    58321 agtggagtca gcattcattt gttctttggc ttcttcttcc gtgtagaatg gaccgactgg
    58381 tttacttgga tttttgaatg aaggatcatt ttcatccaca ataacttgag tgactaaaga
    58441 aaccacatct ttatcgatcc ctttttcctt cagtacttcg cccatggcgt tttgtaacca
    58501 gtaaccgata gatccttctg tcattgcaac acatgtatct aaaggcattg ccggtgtttt
    58561 ctcagaatca gcagcttgct gctgaatcaa taggttaccg acttgcggac catttccgtg
    58621 ggagatgatc aattcatctc cttgttcgat aaatttcact aaatactttg ctgtttccat
    58681 taacgcttct tgttgggctt ttgcacttgc atccgtagac aagatggcat tgccccctaa
    58741 agcgacaact acttttcgtt tggccatttc attcgtcctt tcttcagttc taccgaattt
    58801 tctagataac aaaacgagcc agcgttttgt gattttccct caatgctgac tcgttttttc
    58861 gtgagatgaa tgtggttatg cttaaacgcg agggataaac aagttaccta atgttgctgc
    58921 catgatcgct ttgattgagt gcatacgatt ttctgcttgg tcaaattgac gagcatattt
    58981 gctgcggaag acttcatctg ttacttccat ttcagtgatg ccaaagcgtt ctttcatttg
    59041 ttcaccgtaa acagtgtttg tatcatggaa agctggcaag cagtgcaaga agatcaaacg
    59101 atctgtgttg tgtgtttttt ctaccatttc catattgatt tggtaaggtt tcaacaattt
    59161 gatacgttct tcgaatttat cttcttcacc cattgatacc catacatcag aataaaggac
    59221 gtctgctccg tctacacctt tatcaacatc gtcggtaatc atcaattgtg caccggattt
    59281 ttcagcaaag ccttctgcca ttttgacgat ttcttcttca ggttgtaatt cttttggtgc
    59341 aacgatacgc atattcgctc ctaaaattgc acctgtgact aataaagagt tagccatatt
    59401 gtttcgacca tctccgcagt aagcaacagt gataccttct actgttccaa agttttcttg
    59461 gatagttaaa aagtcagcga tcatttgtgt tggatgccat tcatcagtca agccattcca
    59521 aactggaaca ccagagaatt ctgctagttc ctctaccatt ttttgactga atccacggaa
    59581 ttcgattccg tcaaacatac gtcctagtac tttagctgta tcttctgtgg attctttttt
    59641 acctaattgg atatcatttg cacctagata ttcaggatgt gcacctagat cgatcgctgc
    59701 agtagtaaat gcagaacgag tacgtgtaga tgttttttca aataaaagcg caatattttt
    59761 tccttctaga tagtgatgtg ggataccacg ttttttcaag tctttcaagt gttctgaaaa
    59821 atcgatcaaa tattgtaatt cttcttttgt aaagtctttt tctgctaata agcttcttcc
    59881 ttggaatact gattctttca tattaaacat tctcctttag attgttttta tttaagatta
    59941 aagaggaaat cccgacggca gctgacagta agactgccgt caggaaacaa attacttcaa
    60001 gtcttcacgg acaagtggtt ggctcatgca gcgtggacca ccacgacctc gtgacaattc
    60061 acttgaattg atttctaaca ctttgatgcc atagctacga agcaattcgt tcgatacata
    60121 atttcggtta taagtgacta caacacctgg agcgattgcc aatgtgtttg atccatcatt
    60181 ccattgttct cgaggtgcaa cgatctcatc tccattacca gttgggatca gtacaagatc
    60241 atctaatcca agagcagctt tcatcgtttc gtgtaagtta tctgaatgag taatcttgat
    60301 atcatcacca tctggttcga ttgtaaagac atccatctta ccgtctttac tttgaattgc
    60361 tggatggata gtgaatttat cgtgatctac cattgtaaat acggtatcca aatgcatcat
    60421 tgcacggttg ttcggaattt tgattgccaa cactttttca aatcctgaat tttttgcgaa
    60481 taggtggcgt gctaattttt ctaaagcttt cgcattagtt cgttgagaaa tcccaacagc
    60541 tactacttta tcgcttaaga tcaattcgtc tccgccttcg atatgatctg gattttcacg
    60601 atccaaccat acttcgattc ctttatttgc aaatcttgga tgatgtttca aaatatattc
    60661 tgtaaacatc gattcacgac gacgtgcttc aaaagtcatc ttattgactg tcatcccatt
    60721 tcccattgat gcggaagggt cgcgtgtaaa gtacaagttt ggcattgggt ccatgtaaaa
    60781 tggatagtca tcgtctgctg acagatcata tagactgcta gaacgtactt cgatatcttt
    60841 tgtacgaacc ccagccatga ttttatctac catttcttgc gtttccattg atagaaggaa
    60901 ctcatgaaga ccctctctta cagcattcga tgcaatatga gattcatcca acattttatt
    60961 caagaattgt tctttgacat tgcctgcgtc aatcgcttct gccgctaatt tttccaagta
    61021 aagagtctcg acgccttcat tttgcaatgt cttcgcaaag ttgtcatgtt cttcttgagc
    61081 aatcggaaga tatggaatat catcgaacaa cagacgatcc ataatatctg gtgtcaaatt
    61141 ttccacttct tgtccaggcc gtttcagtaa aactgtcttt aatttcccta tttcagagaa
    61201 aacgtgaata ggtttatcca tgttgtgttg cctccttctt tttgattaca tgtataggat
    61261 atcctccttt tgtaatcact ttcataattt tgtctcattc aaaatgagac attcctcaca
    61321 ttaatattcg ataattaaat aattaaacgt ttttctattt ttaagc
//
LOCUS       DAYYFU010000010        60875 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000010 DAYYFU010000000
VERSION     DAYYFU010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.010"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            199..2733
                     /gene="secA"
                     /locus_tag="ACHA34_000768"
     CDS             199..2733
                     /gene="secA"
                     /locus_tag="ACHA34_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFM8791435.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2859..3960
                     /gene="prfB"
                     /locus_tag="ACHA34_000769"
     CDS             join(2859..2930,2932..3960)
                     /gene="prfB"
                     /locus_tag="ACHA34_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFM8791436.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4065..4751
                     /gene="ftsE"
                     /locus_tag="ACHA34_000770"
     CDS             4065..4751
                     /gene="ftsE"
                     /locus_tag="ACHA34_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFM8791437.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4744..5628
                     /gene="ftsX"
                     /locus_tag="ACHA34_000771"
     CDS             4744..5628
                     /gene="ftsX"
                     /locus_tag="ACHA34_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFM8791438.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            5780..7228
                     /gene="cls"
                     /locus_tag="ACHA34_000772"
     CDS             5780..7228
                     /gene="cls"
                     /locus_tag="ACHA34_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8791439.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7565..8422
                     /locus_tag="ACHA34_000773"
     CDS             7565..8422
                     /locus_tag="ACHA34_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFM8791440.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8537..9457
                     /gene="pstC"
                     /locus_tag="ACHA34_000774"
     CDS             8537..9457
                     /gene="pstC"
                     /locus_tag="ACHA34_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFM8791441.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9457..10341
                     /gene="pstA"
                     /locus_tag="ACHA34_000775"
     CDS             9457..10341
                     /gene="pstA"
                     /locus_tag="ACHA34_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFM8791442.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10356..11162
                     /gene="pstB"
                     /locus_tag="ACHA34_000776"
     CDS             10356..11162
                     /gene="pstB"
                     /locus_tag="ACHA34_000776"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8791443.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11178..11936
                     /gene="pstB"
                     /locus_tag="ACHA34_000777"
     CDS             11178..11936
                     /gene="pstB"
                     /locus_tag="ACHA34_000777"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8791444.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11949..12626
                     /gene="phoU"
                     /locus_tag="ACHA34_000778"
     CDS             11949..12626
                     /gene="phoU"
                     /locus_tag="ACHA34_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFM8791445.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            13203..13895
                     /locus_tag="ACHA34_000779"
     CDS             13203..13895
                     /locus_tag="ACHA34_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8791446.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            13892..14659
                     /locus_tag="ACHA34_000780"
     CDS             13892..14659
                     /locus_tag="ACHA34_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8791447.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14759..16327
                     /gene="liaX"
                     /locus_tag="ACHA34_000781"
     CDS             14759..16327
                     /gene="liaX"
                     /locus_tag="ACHA34_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFM8791448.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16624..16944
                     /locus_tag="ACHA34_000782"
     CDS             16624..16944
                     /locus_tag="ACHA34_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFM8791449.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            16944..17294
                     /locus_tag="ACHA34_000783"
     CDS             16944..17294
                     /locus_tag="ACHA34_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFM8791450.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17461..18399
                     /gene="hprK"
                     /locus_tag="ACHA34_000784"
     CDS             17461..18399
                     /gene="hprK"
                     /locus_tag="ACHA34_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFM8791451.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18414..19244
                     /gene="lgt"
                     /locus_tag="ACHA34_000785"
     CDS             18414..19244
                     /gene="lgt"
                     /locus_tag="ACHA34_000785"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFM8791452.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19288..20313
                     /locus_tag="ACHA34_000786"
     CDS             19288..20313
                     /locus_tag="ACHA34_000786"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8791453.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20328..21266
                     /gene="galU"
                     /locus_tag="ACHA34_000787"
     CDS             20328..21266
                     /gene="galU"
                     /locus_tag="ACHA34_000787"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8791454.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            21391..21831
                     /locus_tag="ACHA34_000788"
     CDS             21391..21831
                     /locus_tag="ACHA34_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFM8791455.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21834..22436
                     /locus_tag="ACHA34_000789"
     CDS             21834..22436
                     /locus_tag="ACHA34_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8791456.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(22495..23601)
                     /locus_tag="ACHA34_000790"
     CDS             complement(22495..23601)
                     /locus_tag="ACHA34_000790"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFM8791457.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            23811..24812
                     /gene="ccpA"
                     /locus_tag="ACHA34_000791"
     CDS             23811..24812
                     /gene="ccpA"
                     /locus_tag="ACHA34_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFM8791458.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(25149..27494)
                     /locus_tag="ACHA34_000792"
     CDS             complement(25149..27494)
                     /locus_tag="ACHA34_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFM8791459.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            27773..28675
                     /locus_tag="ACHA34_000793"
     CDS             27773..28675
                     /locus_tag="ACHA34_000793"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFM8791460.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            28888..30663
                     /locus_tag="ACHA34_000794"
     CDS             28888..30663
                     /locus_tag="ACHA34_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFM8791461.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(30729..32315)
                     /locus_tag="ACHA34_000795"
     CDS             complement(30729..32315)
                     /locus_tag="ACHA34_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFM8791462.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            32566..32790
                     /locus_tag="ACHA34_000796"
     CDS             32566..32790
                     /locus_tag="ACHA34_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFM8791463.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            32932..34683
                     /locus_tag="ACHA34_000797"
     CDS             32932..34683
                     /locus_tag="ACHA34_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM8791464.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            34683..36467
                     /locus_tag="ACHA34_000798"
     CDS             34683..36467
                     /locus_tag="ACHA34_000798"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8791465.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            36793..37644
                     /gene="licT"
                     /locus_tag="ACHA34_000799"
     CDS             36793..37644
                     /gene="licT"
                     /locus_tag="ACHA34_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFM8791466.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            37737..39623
                     /locus_tag="ACHA34_000800"
                     /pseudo
     CDS             37737..39623
                     /locus_tag="ACHA34_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
     gene            complement(39663..40565)
                     /locus_tag="ACHA34_000801"
     CDS             complement(39663..40565)
                     /locus_tag="ACHA34_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8791467.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            40796..42199
                     /locus_tag="ACHA34_000802"
     CDS             40796..42199
                     /locus_tag="ACHA34_000802"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8791468.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            42199..43632
                     /locus_tag="ACHA34_000803"
     CDS             42199..43632
                     /locus_tag="ACHA34_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8791469.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            43777..44703
                     /gene="trxB"
                     /locus_tag="ACHA34_000804"
     CDS             43777..44703
                     /gene="trxB"
                     /locus_tag="ACHA34_000804"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFM8791470.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            44917..46644
                     /locus_tag="ACHA34_000805"
     CDS             44917..46644
                     /locus_tag="ACHA34_000805"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFM8791471.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            46659..47009
                     /locus_tag="ACHA34_000806"
     CDS             46659..47009
                     /locus_tag="ACHA34_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFM8791472.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(47101..47961)
                     /locus_tag="ACHA34_000807"
     CDS             complement(47101..47961)
                     /locus_tag="ACHA34_000807"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFM8791473.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            48053..48481
                     /locus_tag="ACHA34_000808"
     CDS             48053..48481
                     /locus_tag="ACHA34_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8791474.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            48643..48819
                     /gene="rpsU"
                     /locus_tag="ACHA34_000809"
     CDS             48643..48819
                     /gene="rpsU"
                     /locus_tag="ACHA34_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFM8791475.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            48846..49292
                     /locus_tag="ACHA34_000810"
     CDS             48846..49292
                     /locus_tag="ACHA34_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFM8791476.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(49446..50825)
                     /gene="brnQ"
                     /locus_tag="ACHA34_000811"
     CDS             complement(49446..50825)
                     /gene="brnQ"
                     /locus_tag="ACHA34_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8791477.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            51531..52502
                     /locus_tag="ACHA34_000812"
     CDS             51531..52502
                     /locus_tag="ACHA34_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFM8791478.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            52526..54718
                     /locus_tag="ACHA34_000813"
     CDS             52526..54718
                     /locus_tag="ACHA34_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFM8791479.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            54735..55211
                     /gene="ybeY"
                     /locus_tag="ACHA34_000814"
     CDS             54735..55211
                     /gene="ybeY"
                     /locus_tag="ACHA34_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFM8791480.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            55195..55590
                     /locus_tag="ACHA34_000815"
     CDS             55195..55590
                     /locus_tag="ACHA34_000815"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8791481.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            55605..56504
                     /gene="era"
                     /locus_tag="ACHA34_000816"
     CDS             55605..56504
                     /gene="era"
                     /locus_tag="ACHA34_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFM8791482.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            56647..57459
                     /gene="recO"
                     /locus_tag="ACHA34_000817"
     CDS             56647..57459
                     /gene="recO"
                     /locus_tag="ACHA34_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFM8791483.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            57843..58760
                     /gene="glyQ"
                     /locus_tag="ACHA34_000818"
     CDS             57843..58760
                     /gene="glyQ"
                     /locus_tag="ACHA34_000818"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFM8791484.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            58762..60837
                     /gene="glyS"
                     /locus_tag="ACHA34_000819"
     CDS             58762..60837
                     /gene="glyS"
                     /locus_tag="ACHA34_000819"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFM8791485.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        19863 a        10760 c        13027 g        17225 t
ORIGIN      
        1 aaaacttgca gcagctactg acagctgccg caagtttttt tgagtttcct cctcttttag
       61 atgaaatatg aataaattat gaagttctta ctttaatacg cttaagatat ttcaattatc
      121 tataactaat gataaaatga aaagtaaagt ctacatagtc tggataatac acagaaggaa
      181 aaggagatta cacaacgcat ggccaatttg attcgttcac ttatcgaaaa cgataagaaa
      241 gaactaaaac gtttagatag cattgcaaaa aaagttgaag catacgctga ccaaatggca
      301 gcattaactg atgaacaatt acaggcaaaa actgctgaat tcaagggcag ataccaaaaa
      361 ggagaaacat tagatcaatt acttccggaa gcttttgcag tcgtccgaga agcagcaaaa
      421 cgtgtattgg ggttatatcc acaccacgta caattgatgg gtggaatcgt gcttcacgat
      481 ggaaatatcc ctgaaatgcg tactggtgaa ggtaaaacct tgaccgcaac gatgccagta
      541 tatctaaatg ctctgactgg cgaaggggta cacgtagtta ccgtcaatga atatttagca
      601 acccgtgact cgacggaaat gggcgaactt tataatttct taggattaac agtggggttg
      661 aatatcaact caaaatcttc ggatgaaaaa cgcgaggctt ataattgtga tatcacttat
      721 agtacgaata atgaattagg atttgactac ttgagagaca atatggtcgt ttaccgccat
      781 caaatggtac aacgtccgct gaattatgcc attgtcgatg aagttgactc aatcttgatt
      841 gatgaagcga gaacgccgct gatcatttca gggcaagcag aaaaatccac agctttatat
      901 acaagagcag acaacttcgt gaaacgtttg aaagaagaag aagattataa aatcgatgta
      961 caatcaaaaa caatcagttt aaccgaagca gggatcgaaa aagcagaaga aacattcggt
     1021 ttggataact tatacgacat tgaaaatact gcattgaccc atcacttgga tcaagcattg
     1081 agagccaact atatcatgct tttagatatc gattatgttg tccaagacgg aaaagtactg
     1141 attgttgacc agttcactgg tcggatcatg gatggacgcc gttattcaga tggacttcac
     1201 caagcaatcg aagctaaaga aggcgtagag atcgaagacg aaacgaaaac catggcgacg
     1261 atcactttcc agaactattt ccgtatgtac aagaaacttg cgggaatgac tggtacagct
     1321 aaaacagaag aagaagaatt cagagaaatt tataacatcc aagtatacca gattccgaca
     1381 aatcgtccgg ttatccgtga tgatcgtcca gatttgcttt acccaacatt acaaagcaag
     1441 ttccacgctg tcgtgcaaga tatcaaagat cgctaccgca aaggtcaacc tgtgttagtc
     1501 ggaactgttg cggttgaaac atctgagtta ttatcagata tgctgaatca agaacgtatt
     1561 cctcatgaag tgctgaatgc gaaaaatcac tttaaagaag cagagatcat catgaatgct
     1621 ggtcaaaaag gtgctgtgac gattgctacc aacatggctg gtcggggaac agatatcaag
     1681 ttaggacttg gggtaagaga acttggtggt ctagcagtta tcgggacaga acgacatgaa
     1741 tctcgtcgta tcgataacca gcttcgagga cgtgcaggac gtcaaggaga ccctggtatg
     1801 tcacagttct acttgtcatt ggaagatgat ttgatgaaac gattcggttc agaacgaatc
     1861 aaatctttct tagatcggat gaagatcggt gaggaagatg ctgttatcca aagcaagatg
     1921 ctgacgaaac aagtagaatc tgcacaaaaa cgtgtagaag gaaataacta tgacacacgt
     1981 aaaaacgtct tacaatacga tgatgtgatg cgtgaacaac gtgaagtaat ctataaacag
     2041 cgacaagaag taatcatgga agaaaaaagc ttatccaagc cactaatgaa catggtcaaa
     2101 cggacaatca gtcgtgtagt agatgcgcat acacaattag agaaagaaaa ttggaatttg
     2161 gaaagtatca tcgattttgc cggcagtact cttgtccatg aagatagtat tactcttgca
     2221 gatatcgaag gaaaaacagc tcaggagatc aaagagtatc taaacgaacg agcacaagaa
     2281 gtatttaata ccaaagcggc tcaactaaat ggtccagagc aactgttgga atttgaaaaa
     2341 gtcgtgatct tgcgtgttgt ggactctaaa tggacagacc atattgatgc aatggatcaa
     2401 ttacgtcagt caatcggctt acgtgcttat ggacagaaca atccattagt cgaatatcaa
     2461 acagaaggct ataagatgtt tgaagatatg gtaggcgcca tcgagtatga agtcactcga
     2521 atctttatga aagctgagat ccgtcaaaac gtacagcgtg aacaggtttc tcaaggaaca
     2581 gcttctcact cagaagacgg agatgccaaa gaaaatagta atactagcgc gaaaaaacag
     2641 ccaatccatg tgaacaaggt cggaagaaat gatccttgtc cttgtggaag cgggaaaaaa
     2701 tataagaact gtcatggaaa aaatgcaaac taatttctga cagcaggatt ggtgagacaa
     2761 attctttttt taagaattcg tctcaccttc tttctgttat ttgctgattc tgttagacta
     2821 gatataatga aaaaagtata gtgaaagaag gaaaaaacat ggaaatatcc gaaattcgaa
     2881 atctcctgac agaaatgcag gaaaaggtca caagcttcag gaggtctctt tgacttagac
     2941 cagctggaag aagacattgc ggaagcagaa gcaaaaatgg ctgaacctgg tttttgggat
     3001 gatagcgaag ctgctcaaaa agtgatcagt gaaaacaatg caaataaaga aacttatgat
     3061 caattccatt ctcttgctga agaactggaa gaaatcgaat tgatggtgga gatgttgcag
     3121 gaagaacctg atgcagccat gcaagaagaa gcagaaacac gtatcaatgc gctagatgaa
     3181 aaaatgaaga catatgaatt atctatgttg ttagatggtc cttatgatcg aaacaacgcc
     3241 atcgttgaat tgcatccagg ggctggcgga acggagtccc aagattgggg cagtatgctt
     3301 ttacgtatgt atacacgctg ggcagagcag catgggtatc aagtagagac actagattat
     3361 caagcaggtg atgaagcagg aatcaagagt gtgaccctac tgatcaaagg acacaacgca
     3421 tttggttatc tgaaatcaga aaaaggtgtt catcgccttg tacgtatctc gccttttgac
     3481 tcagcgaaac gacgtcatac ttctttttgt tcggtagatg tgatgccaga gcttgatgaa
     3541 acgatcgata tagaaatcaa tccagatgat ttgaaaatcg atacgtaccg agcaagtggt
     3601 gcaggtggtc agcacatcaa taaaacagaa tctgctgtac ggatcactca cttgcctaca
     3661 ggaacagtag tagctagtca ggcacaacgt tcacaattga aaaaccgtga gcaagcgatg
     3721 gggatgttaa aagctaagct ttatcaatta gaagtggaaa agaaagaaca agaagcagct
     3781 gcacttcgag gagaacaaaa agaaatcggc tggggttcac agatccgttc ttatgttttc
     3841 catccatatt ctatggtaaa agaccatcgg acaaacgtag agacaggaaa tgtccaagca
     3901 gttatggacg gtgaccttga tttatttatt gacggctatt taaaaatgca tctacaataa
     3961 gaacccattg ttaaattgaa ataaaattgt aagattttgc aagcatgttg aaacattgcc
     4021 atgctataat agctaaagac tgaaaaagat atggagagaa cgccatgatt gaaatgaagg
     4081 atgtaatgaa gaagtactca aatggtacga ccgctatccg caatctctca attgtcatcg
     4141 aacaaggaga gtttgtctat gtggttggtc cgtctggagc cggtaaatct acattcatca
     4201 aattgatgta tcgagaagaa aaagcttcaa aaggaacttt gaacgtcgca ggtcatgatc
     4261 taatagcaat caaaaaccgc gaagttcctt atctacgcag agaaatcggc gtggtgttcc
     4321 aagactataa attattacca agaaaaacag tatatgaaaa tgttgcgtac gctatgcaag
     4381 ttatcggcag aaaacctcgg gatatcaaaa aacgagtaat ggaagtgctg gaccttgttg
     4441 gtttgaaaca caaagttcga gtctttccta gcgaattatc cggtggggaa cagcaacgtg
     4501 tctctattgc tcgggcaata gtgaatacgc ctaaagtact tatcgcggat gaaccgacag
     4561 gaaatctgga tccagaaaac tcatgggaaa tcatgaaatt attagatcgg atcaatgccc
     4621 aaggaactac tgtcgtgatg gctacacata acagtacgat cgtaaatacg attcggcacc
     4681 gagtaattgc tatcgaaaac ggccgcatta ttcgagatca agcggaaggg gaatatggat
     4741 acgatgatta gaaccttttt ccgccattta ttagaaagta tcaagagttt gaaacgtaac
     4801 ggctggatga cagttgcttc agtcagtgcg gtaacgatca cattgacttt agtcggtgta
     4861 ttcatggcag ttatcatgaa cgcaactaaa ttagctcaag atatagaagg aaacgttgat
     4921 gtctctgtat tcgtagatat cggtacaaaa gacgaagaga tgaaaaagct agaaaaagaa
     4981 ttgaatgact tagatcatgt taaaaaagtt gaattttcaa gcaaagaaca ggaattgaaa
     5041 aaaatccaaa acgaaatggg tgattcctgg aaattgtttg aaggtgacaa taacccgtta
     5101 tatgatgtgt atgtagtaag tgctactgat ccttcttata caaaacaagt agcagaagaa
     5161 gcagcagatt tgaagaatgt ttctcgttct gattacggtg gagcttcctc agatcggatt
     5221 ttccaaatcg ctggtgcagt tcggacatgg ggattagttg cagcggcatt gctgctgttc
     5281 gtagcgatgt ttctcatttc gaacacgatt cggatcacga ttttgtctag acagcgtgaa
     5341 attcaaatca tgcgtttagt cggtgcaaaa aatggatata ttcgctggcc gtttttcctt
     5401 gaaggtggct ggatcggtct gatcggtgcg attcttccga ttttgatcat cacatttggt
     5461 tatgcatggt ttttccaatt gatcaatcca tcattattac gttcccatta ttcattgatt
     5521 catccacaaa atatcgtttg gaaaatcaat cttttaatgg tcggcattgg tgtgatcatt
     5581 ggttcacttg gctcgatcat ttctatgaga cgtttcttga aattctaata ccacaatata
     5641 aataaaatga gaaagttgga cagtttcgtt ccagctttct ttttttattg gctaggagaa
     5701 tagaaggaag cggctttttt ccctttactt taaaaaacaa cttgggtatt cttaagttgg
     5761 tagaagaaga ggtgaagccg tgaaaattat cgttgacaat ttttttacga tccttttgat
     5821 tatgaatatt ttgctttcct ttatcatcgt ttttcgagaa agaaaggaaa ctgctcaaac
     5881 atgggcatgg gtgctcgttt tgatgttcat tccagttgta ggatttattt tatatatttt
     5941 tttaggaaga gggatttcca aagagaaaat ctttgatttg aagatccaag ggaaaatcgg
     6001 taagaacctg gaaattgaag aagacaaaca ggcattgatg cgagggcttt atcctcatcc
     6061 gcctactgga caagtagatg tgaaacaatt gatctatatg ctcacggtat ttgaaagtac
     6121 gctctatacg actaaaaatg aaatcacttt atttacagat ggtcgtgaaa aattcgatgc
     6181 gctgattgaa gatatcaagc aggcagaaga ccatatccac ttccagtatt atatttatcg
     6241 cagtgatgca ttaggtgaag aagttcgaga tgcattgaca gaggcggcaa gacgaggggt
     6301 aaaagtcaga gtccttcttg atgcatgggg ttccacacaa gtttcttcca gcttttttca
     6361 gaatttgaaa aaagcaggcg gagaagtcgc atttttcttt cctctatttg ttccgtatat
     6421 taatcctcgg atcaattatc gcaatcatcg taaaatcgta gttattgatg ggaaaatcgg
     6481 atatacaggt gggttcaatg ttggaaatga atatctagga ttggtaaaga aatttggtta
     6541 ttggcgagac aatcatttgc gtatttacgg agaagcggtg tataccttgc aaaaccgttt
     6601 tttgatggac tggaattctc agcatacaaa agaagtaggc tattctccca aattttttcc
     6661 gtccattcac tctactggtg atatagctgt tcaaatcgtg acaagcggac cagataccga
     6721 acatgaacaa atcaaaatga cttatttgaa aatgatttcg ttagccaaac gggagatatt
     6781 gatccaaacg ccttattaca taccggatgg atcgattcat gaagcactga agttggctct
     6841 tctttcaggc gtaaaagttc atatccaaat acctaacaaa ccagatcatc ttctcgttta
     6901 ctgggcaacg tattctttcg ctgcagagct aatcgagtat ggagcaagga tcgaaacata
     6961 tgagaatgga tttattcatg caaagacgat gattatcgat ggtggcattg tttcagttgg
     7021 ctcagcaaat atcgatgttc gttcatttcg cttggacttt gaggtcaata cactggttta
     7081 tgatgaacga atggcttcac gtgtaagaaa agcttttttt gaagactcga aaatttctac
     7141 gcatctcaca aaagaaatgt atgaaaaccg aggaataatc atcaagatga aagaaggatt
     7201 agcaagattg atttcgccgc tcctttaaaa cataaaaagg acatgagaac catcaacaga
     7261 tggagatctc gtgtcctttt ctaacggggc catttctgag agtacgtcag aaatggagaa
     7321 agcgtctcgc atgagcgaaa ttaactttgg ggtatctctt gttttttggg gaaagcaaga
     7381 aataagctaa tatatgctat taaaagagac acatttcaaa gccagtatag catagaaact
     7441 tttaatgaga tagttttttt tacaattatg agaatttatt ttgttattta atgattttga
     7501 ttcgttttca tcaagtaatg aaaatgataa aataaacata ctttaggatt aaaggagatt
     7561 tctaatgaaa aaacttaaat tattcttacc aataatagga ttagctattc ttttatcagg
     7621 ctgttcaaag tggattgatc gaggagaatc cattacggct gttggctctt ctgctttaca
     7681 gcctttagta gaaacagttg ctgaaactta ccagacagaa aatcctggaa aattcatcaa
     7741 tgtacaaggg ggcggaagcg gaactggttt gtctcaagta cagtcaggag cagtcgacat
     7801 cggaaactct gatttgtttg cggaagaaaa atcggggatc gatgcctcag cattagtcga
     7861 tcatcgagta gcagttgttg ggatcactcc tattgtgaac aaggatattg gcgtaaaaga
     7921 catcagtatg gaaaacctga aaaaactatt tacaggcgag atcaaaaact ggagtgaagt
     7981 cggtggaaaa gatcagccag tagttatatt gaaccgcgct tcaggaagcg gaacccgtag
     8041 cacgtttgaa aaatgggtat tggacggaga aacagctgtt caggcacaag aacaagattc
     8101 aagcggaatg gtgcgccaga tcgttgccga cacacccggt gcaatcagct atgttgcttt
     8161 ttcttatgtt tccgatgatg tagatacgtt gaatgttaac ggtgtagaac cgacagatga
     8221 aaatgtgaca acgaacaaat ggacgatttg ggcatatgag catatgtaca caagagatca
     8281 tccaaaaaaa ctgacaaaag aatttttaga ctatatctta tctgatgaag tacaagataa
     8341 catcgttgga gaactaggtt atatccctgt ttctcatatg aaagtcgaac gtgattggca
     8401 aggaaatatc gttaaagagt aatctttaca caaaatttac aaacttagct aaacttttac
     8461 atgttattaa aaccaaattg atgttcaatt tgtacactga gtgagtatgt taaacctgat
     8521 taagggggag tcatctgtgg aagatgtgaa aaaagtatta ttaacaaaat caaaacgttc
     8581 aaaaatggaa cagcgtggaa aattcatcag ttttttatgt atcgcgctga tcgtgttggt
     8641 tgtattatct attttttatt ttgtagctag taaaggattg gccacctttt ttactgataa
     8701 gatcaatgta tttgattttc tgtttggtac agattggaat ccaagccaaa tcggagcaaa
     8761 tgggaaacca atggtagggg cattgcctat gatcacaggt tcgtttattg tgaccttttt
     8821 atctgctatc gttgccacgc catttgccat tggtgcagca gtattcatga cggagatctc
     8881 tcctaaaaac ggaacgaaga tcttacagcc ggtcatcgag ctgcttgtag gtattccttc
     8941 tgtcgtttat ggttttatcg gtttatcagt tatcgtgcct tttgtcagaa gtatttttgg
     9001 cgggactggg tttggtatct tagctggtac atttgtgttg ttcgttatga tcttgccgac
     9061 tgtcacaacg atgactgttg atgcattaaa ggcagtgcca cggcactatc gggaagcttc
     9121 attagcgctt ggtgccacaa gatggcaaac aatctacaaa gtcgtgctac gagcagccgt
     9181 gcccgggatt ttaacagcag tcgtttttgg tatggctcgt gcgtttggtg aagccttagc
     9241 tatccaaatg gtcatcggta atgcagcatt gatgccaaca ggtcttacta ctcctgcttc
     9301 tacattgacg tccatcttaa caatgggaat cggaaataca atcatgggaa cagttgaaaa
     9361 taatgtgtta tggtcattag cgctgatctt gctattgatg tcattattct ttaatatcgt
     9421 gatccgtatg attgggaaga aaggagagct gaaataatga acgcaaaacg ctcagataaa
     9481 attgctacag gtgttttata tatagtttcg gggatcatcg tattgatttt agcagcttta
     9541 ctactttata tcttagttcg gggaataccg cacatttcat ggtccttctt gaccgaacct
     9601 tccaaagcct accaagaagg cggtggaatc gggatccagc tatttaactc gatttatttg
     9661 cttttgatca cgatgatcat cagttttcct atttctttag gtgcgggtat ctatctttca
     9721 gaatatgctg gaaaaaactg gctaacagat gtgattcgca cgtcgattga aatcttgagc
     9781 tcgttgcctt ctgtcgttgt cggtttattc ggtttcttgg ttttcgttat tcaattccag
     9841 tatggtttct cgattatttc tggtgcattg gctttgactg tattcaactt gcctcttttg
     9901 actagaacag ttgaggagtc tttacaagcg gtccattaca cacaacgaga agcaggattg
     9961 gccttaggtt tgtcacgctg ggaaacagtg ataaaagtgg tggttccaga agcgacacca
    10021 gggattttga ctggtgtgat cctaagttct ggaagaattt ttggtgaagc agctgcattg
    10081 atctatacgg ctggacaaag tgcacctgcg ttagatttta cgaactggaa tccattaagt
    10141 gtttcaagcc cgatcagcat tttccgtcaa gctgaaacat tagctgtcca tatttggaag
    10201 atcaatacag aaggcacgat gcctgatggt gtttctgttt cggctggcgc ttctgccgtg
    10261 ttgattattg ctgttttact gttcaacttc ggcgctcgtg cgattggcaa acgattatac
    10321 cgaaaaatga cttcagccta aggagaagaa gcaaaatgaa aaaatataac tggaatgaaa
    10381 caaatgtcat caccatgcca aaagagaaag aagtagcact atatacggat gatctacatg
    10441 tctggtatgg cgacaatgaa gcaatcaaag gagtggatct ggaatttgaa aaaaataaga
    10501 tcactgcact gattggtcct tccggctgcg gaaaatctac gtatcttcgt tcgttgaatc
    10561 gaatgaatga tgggatcgct aatacgaaaa tcactgggaa aatcatgtat caaggcgtag
    10621 atattaatgc gcctcaagtg gatgtttatg agatgcgcaa acggattggc atggtttttc
    10681 aacgtcctaa tccgttcagc aagtcgattt atgacaatat tacatttgct ttgaaacagc
    10741 acggcgaaaa aaacaaaaag aaattagatg aaatcgttga aacaagcttg aaacaagctg
    10801 ccttgtggga ccaagtaaaa gacagcttgg acaaaagtgc cttggcttta tctggcgggc
    10861 aacagcaacg tttatgtatt gctcgtgcga tcgcgatgaa acctgatatc ttgctgctag
    10921 acgagccggc aagtgctttg gaccctattt cgacaggaac ggttgaagaa acacttgtaa
    10981 acttgaaaga agactatacg atcatcatcg tgacgcataa catgcaacaa gcagctcgta
    11041 tcagcgacta tactgccttt ttctatttag gaaaagtaat ggaatatgac aaaactaaca
    11101 agatatttac cagaccaaaa attcaggcaa ccgaagatta tgtgtccggt cattttggtt
    11161 aagacggagg cctcaacatg acaaaagaga ttatttcatc taaagatctt catctgtatt
    11221 atggaaagaa agaagcacta aaagggatcg atctaagctt caatcaaggg gaaatcactg
    11281 cgatgatcgg tccttctggt tgcggaaaat ccacttatct gcgttctttg aaccggatga
    11341 atgacctgat tccgaatgta acgatcaccg gaagtgtttt atataaagac aaagacatct
    11401 atggtccaaa aacagatgtt gttgaactaa gaaaagaaat cggcatggta ttccagcagc
    11461 caaacccatt tcctttttct atctatgaaa atgtcatcta tggattgaaa ttaaaagggg
    11521 aaaaagataa aaaagtcctt gatcaagtag ttgaagaaag cctgaaggca gcgtctgttt
    11581 gggatgatgt gaaagacaaa ttgcataaaa gtgctttatc tttatccggt gggcagcagc
    11641 agcgtgtatg tatcgcacgt gttttggcag ttaatccgga aatcatctta ttggatgaac
    11701 caacaagtgc gcttgatcct gtttctactg ggaaaatcga aagtatgctg ttagaattaa
    11761 gagagcggta tacgatgatc atcgtgaccc ataatatgtc tcaagcttca cgtatttcag
    11821 ataagacggc attcttttta gatggtcact tgatcgaata caatgatacg aagaagatat
    11881 tcatgaatcc agaaaaacaa gagacagaag attatatttc aggtcgattt ggataaggag
    11941 gaaacggaat gctacgtaca caatttgaag aagaattgct gaatctccat aatcagttct
    12001 acgaaatggg gatgatggtg agcagcgccg tgcacaagtc agttcgtgca tacatcaaac
    12061 acgataaaat cttagcccaa gaagttatcg aaaacgatat aaatatcaat aacatggaaa
    12121 cacgcttgga gaagaaaagt tttgaaatga tcgcattgca acagccagtt acaactgatt
    12181 tgcgtatgat cattacggtc atgaaagcta gctcggattt agaacgtatg gccgatcatg
    12241 cggtgtctgt tgctaaatcc acgattcgcg taaaagggca gacgagaata ccggaaatcg
    12301 aaaaagagat ctccgatatg tccgattatg tgaaaaaaat ggtggacaac gtcctagttg
    12361 cttatgtcaa aacagacgaa aaagacgcac gtacgatcgc caatatggat caaagagtca
    12421 atgactattt caatcgaatc tataatgcaa caatcaaaaa tatgcaggaa aatcctgaga
    12481 cagtcatcag tggaacagac tacttgaatg ttgccagtta tctagaacgg atcggagact
    12541 atgtgacaaa catctgtgaa tggatcgtct acttggctac agggaaaatc actgagttaa
    12601 atacgaacca caatgagatt ttctaaactg aagctgtgat tatgccgttt gtttaggaaa
    12661 agaggttgtg aaatcagcgg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagtc
    12721 aaacaaaaaa tagctcctca tatttttcgt ttggctggac ttattaccgc agatgcaagc
    12781 ttttgaacac cgtttattcg ggaaagggtt tgtgacaaaa catgtcacaa acccttttca
    12841 tcatttcata gaaaggataa agaatgcaaa aaaaaattag catcatcact atatttttta
    12901 tttgtgtgct tacttcatgg gttattcagc atcaagccgc tttatatgaa gtacctgtca
    12961 tcgagataga aaaagtacga ataaaagagg atcatcaatt gattacagga aaactgatga
    13021 acacggatca aaaaggagaa tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag
    13081 aaacagcaaa tataaaagcg agacaaatgt atctagtcca aatgcatgga gatgaatggc
    13141 agctagtagc aaaaaaaaat gatggctggc ttttcttctt cacaagtatc ttcattggcg
    13201 ccatgttgct tattggtgga aaacagggtc tatttgcttt aggaagtctc atcatcaata
    13261 tcgttttact tttacttttg ttgctcatct ttactaaaac agaagggatt tctctattaa
    13321 atatatctat cctgttcgtc gttataggga tgattattag catattggct ttagatggat
    13381 ggaatagaag cagtataatc aaaatttcag ctgcagttac gagtgttttt ttagcatttt
    13441 ttatttgtct tttgacaatg aatcattgga aagaccaagg actgcgctat gaggaactga
    13501 attatctgac acgaccgtat cgttctgttt ttctttctag cgtactgatt ggaacagctg
    13561 gaggaacact cgataccgtt atcacagtca ttgcgacatt agaagaattg actaagcgac
    13621 aaccagaaat aacagcgaaa cagttatggc tttcaggaag gcaagtgggt cgagatgtga
    13681 ttggctcgat gaccaatatc cttttgttcg tctatatcag tgggtcagtt ccatcgcttc
    13741 ttttatattt aggaaatcaa tggtcgttca aagaaacgat agaacagcat ttatcattgg
    13801 aatttttacg cgtcattgct ggcagcttag gaatagtatt atcgatccct atctcggtcc
    13861 tcttttttct gactgtaagg aggctgaaac gatgaatgta cttattatac ttgttgggat
    13921 tttagcaatc agcgtgctac ttattggcgg tacgcaaggt atgtatattc ttttaggctt
    13981 attcatcaat ttagggatct ttttcctgtt gttattcggt tatcatcaga agtggccgat
    14041 actggcattg tcggtcatag gatttttatt gattgctgtg gttatcttat tttttatcaa
    14101 cggttataat ctaaaaatgc gtgcagcttt tgtgagtata ctgatctttt tattttgttt
    14161 cctactattg cttcctatca ctgatttttt agcaatccaa ggttttacat cggtagaact
    14221 agaagaatta tctggcttag ataagacttt agcgattgat tttagattac ttacgagatc
    14281 gttgcttctg attagtctta gtggtgctgt tttagatgcg agtgtagcaa tttctagtgg
    14341 gacattcgaa gtatatcagg caaatccgca attatctttt taccaattgc gccattctag
    14401 tttcgctatt gctaaaaaaa tattggcgtc aacagtaatg acacttcttt ttgcattcat
    14461 gggaagttct ttagcgttga tcctctggtt tattgattta gacattcctt ttcagcaagt
    14521 gatcaatgaa aaatcatttg ttctagaata tacgatggct attcttacca cattgtccgc
    14581 actacttgtt cttcctttaa cctgcgtgac tacagcatat ctctttacaa aaaagaaaga
    14641 acaactcaag atggagtaaa agttacaact taagtcctat gacattttta ctgaatcagt
    14701 gcataatatg gatgtaagga aaagcttgca aacaattttg cttaaggagg caatttccat
    14761 gaaagaaaga gaacgtattt tagatttagt gaaaaaagga atcctttcga ctgaagaagg
    14821 cttagattta ttagaaagca tggcaactga aaaagacgaa aagcaaatca aaaaagaagc
    14881 agacaaggta actgctagtc ataaagaaaa agatcaggca agtcaattga tcgataaact
    14941 tgaaaacggc gaagaagaaa tttctgagcc agttgatcca aaggaaagag aaagacaaga
    15001 tcaagaaaac ttagaaaaga tcttagatga actagcaact gaagcgaata aaacttcggc
    15061 gcgtctagat gaagtaaatg ctcaattcgc tgacaataag gcagcacgca atgagaaaca
    15121 ggaagcattg atgcagctga atacaaaaga agaattaggc gagttgacag aagaagaatt
    15181 agcacagcgt cagacgttag aagcagaaat caaagaatta gaagctagtg gcgatacact
    15241 gttagaagaa caattgaaat tagaagcaga attaaaagat atccgtaaga atcaatggtc
    15301 tgagaaaaaa gaaacgttta ctgataagtt tgaattgcca gatgattgga aagatcaagc
    15361 aacagataca ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac agttaggcaa
    15421 attcttgaag aaaacattcc aaactgtttc agaaacagtg aatgacaaca tggaatggaa
    15481 agatgtcagc ttacgtgtcc ctgggattgc gacaacgaaa tttgagcatg aattttacta
    15541 tgaagctcct gcagcaagta tccttgatat caaggcagca aacggaaacg tgacgttaaa
    15601 aacgtgggac agcaacgatg tgaaagttga agctaagatc aaactttacg gaaaaatggg
    15661 tgctgaacca tttgaagcat tctctgaacg gagccagatt gaggttaatg aagatcatat
    15721 ctccttccaa attccaaata aacgtgtgcg tgcagacctt gttttctact tacctaaacg
    15781 agtatacgac cacgcagcta ttaaacttct aaatggcaac atcatgattg aaacattgga
    15841 agctaaagat atttatacaa aatctacgaa tggtaacatc atcgtggacc aattaactgc
    15901 aacgatgctg gaagtagaag gtgtaaacgg caatatcgat attcgaaacg ggaacatctt
    15961 agattctatt atcgaaacag taaatggtac agtgacattt ggtgcgacag cagaaaacct
    16021 ttctgtgtct ttggtaaatg gtgatgtacg tcttacgatt aaagaagata atttgaaaaa
    16081 agtagaagca agttcagtga acggaaatgt caaagtcgct ttaccagatg gcatcggttt
    16141 ggaaggacat gctaaaacaa gtcttggaag tatcaacagc cgtctgtcca attacgaagt
    16201 agttcgtgag aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg
    16261 tgaaatcgcg caagtgcaac tttcaacgac aacaggaagt atctatttaa aagatacaga
    16321 taaataaaga ggttgtgaaa gagatgttca gccttgagca ataagaagcg attttgtaaa
    16381 atagcttccc ctattttaca aaattgtgcc ttattgccga tagtctatct cttgaacacc
    16441 gtttaccaag agattatgaa cctgccaatt atcgccgagt cacaaggaat aatttcagaa
    16501 aaagctcgtc acgttttatg aatatggtaa tttgtgaccg agaattttgc agaaagacac
    16561 cgattaagaa gaaggttgtc acaacgattt tcttcattta aaatagtaga acagaggtga
    16621 gacatgatga aaaaaaagtt gacaaaatct aataaaaatg ttgttttgac agggacttta
    16681 gcaggaatag gagaatacat cgggattgat ccgactgtta tccgtgtgct ttatgtattc
    16741 ctgagcttag ttgcttttgg aagtccaatt attttataca ttttattagc tttgattatc
    16801 ccatctgcgc gttctgcacg aacaaactat ggacataaca atgcatatta tgaaaaaaac
    16861 aattatcgtg aacaaactcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
    16921 gatgacgaat ggagtgactt ttaatgtcgt actttcaacg tattttagtt aatacgttaa
    16981 cattcatatc attggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
    17041 ccgttcttgc cagcttcgta ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga
    17101 tactgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
    17161 cgattcttaa aatgacctcc ttttttgtgg gagcagaaca ttttagtttt tcttcattcg
    17221 gtgcagcgtt gttagtagct atcttgatgt cgcttgtcaa tgtaattgtc agcgaacata
    17281 acagtagaaa ataaaagaaa gctgtgccga actatcggcg cagttttttt atttagaatc
    17341 aggaatgcgt tttctattac attggtaatt aaataaaaac gtttttttta attggtagta
    17401 cttttggcta gacaatgata aaatgataga gaacactcta atagaaatga ggtagcatga
    17461 atgtcagaag ttgtaaaagt tagccaatta gtgaaaaaat tatctttgga aatcgtgaca
    17521 ggcgacgaga aaagcttaga ccgtgtcatc gtaactgggg acatctcacg cccaggacta
    17581 gagttgactg gttattttaa ttattattcc cataatcgtt tacagctatt tggcagtaaa
    17641 gaaatcagtt ttgcggaacg gatgatccca gaagagcgtt tgatcatcat gagaagatta
    17701 tgtagtgaag atatgcccgc atttattatt tcaagaggat taccagctcc agaagaaatg
    17761 atccaggcag caaatgaaaa tgggatagct gtactgcgtt ctcccatctc aacttctcgt
    17821 ttattaggag aattatccag ttatttagat ggtcgccttg cccctcgaac aagtgtccac
    17881 ggtgttttag tggatgttta cggattaggt gtactgattc aaggtgacag tgggatagga
    17941 aaaagtgaaa cagcgttgga gttgatcaaa cgcggccatc gattgatcgc agacgatcga
    18001 gtagatgttt atcagcaaga tgaattaacg gtgatcggag aaccgcctaa aatcctggaa
    18061 catctgattg aaatccgagg aatcgggatc attgatgtaa tgaatctttt tggtgcgagt
    18121 gcggtacgtg gctctatgca agtccagttg gctgtttatc tggaagcatg ggcaaaagat
    18181 aagaaatacg accgtctggg ctctgaagac acgaccgttg aaatcgcgga agtgggcatt
    18241 ccacaaataa aaatccctgt caaaacagga cgaaacgttg caatcatcat tgaagtagcc
    18301 gcgatgaatt tccgcgcacg tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga
    18361 ttaagtcgct tgattgaaga aaactcaggc aacgactagc aaggagtgac aatatgttag
    18421 cacaaatcaa tcgagttgct tttgagcttg tagggattcc tatctactgg tatgcattga
    18481 tcatcgtatc aggtatcatt atcgctatgt ggcttagcag cagagaggct gttcgggtag
    18541 gattaaaagc agatgatgtg acagatttta tgttgtgggg attgcctatc tccattattg
    18601 gcgctcgctt atattatgtt ttatttgatc ttccccagta tatagctgat ccaatccaaa
    18661 tattcaatat ccgttcagga ggcttagcta tttatggcgg actgattgca ggcgcgatca
    18721 cgctgtattt ttttactaaa tatcatttta tttctttgtg gactttttta gatatcgctg
    18781 caccaagtgt attattagca caggcaatcg gtcgttgggg aaatttcatg aaccacgaag
    18841 catatggacc agacacgacg cgaagcttct tagaaagtct gcatttaccg caattcatca
    18901 ttgataatat gtatatcgaa gggatctatc gtcaaccaac atttctttat gaatcacttt
    18961 ggagtttgat tggatttatt ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa
    19021 aaggcgaagt ccttttatgt tatctaatgt ggtatagttt cgggcgtttc tttattgaag
    19081 gattacgtac agacagtctt tactttttag gtgtgatccg tatttctcaa ttattatctg
    19141 ctgtgttatt tgtcggtaca ttgattctat ttatttggcg cagaaaaaaa ggaaatttac
    19201 cggattatga ccgctcatta ggaaaaaatc aacaaattat ctgattttta gagcgttaaa
    19261 aatgaaaaaa atttcaggag tggtaaaatg aattatagag tagcggtttt aggaccaggc
    19321 tcttggggta ctgctttggc acaagtcctc gccgaaaacg gacatgacgt tcggatttgg
    19381 ggacatcgtg cttcacaagt agaagaaatc aacacccaac acacgaatca acgttattta
    19441 ccagatcatt atctacccga ttccatccaa gcattcacca atatggaaga tgcagtaaaa
    19501 gacgcagatg cggtgctatt cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa
    19561 ctagtgccta aattgaatac aaagccagtg atcatccatg caagtaaagg attagaacag
    19621 ggaacacata aaaggatctc tgacatcttg ttagaggaaa tcccagcaga aaaaagaaaa
    19681 gctgttgttg ttctttctgg accaagccat gcagaagaag tagcaaaaca tgacatcact
    19741 acgatcacag ctgcaagtac agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg
    19801 aataattatt ttcggatcta taccaatcct gatgtgatcg gtgtagagat gggggcagct
    19861 ttgaaaaata ttattgctat tggtgctggc gctattcacg gcttagggtt tggcgataat
    19921 gcaaaggcag caatcatgac gcgcggatta gctgagatca gccgtttagg tgtagcaatg
    19981 ggtgccaacc cgctgacctt tatcggatta agcggaatag gcgatctagt cgttacttgc
    20041 acaagtgttc attctaggaa ctggcgcgca ggcaacttgt taggacaagg gcagccccta
    20101 gaagaggtgc tagccaatat gggaatggtc gttgaaggtg tgaatactac ccaagcagcg
    20161 atggaactag cccaaatgat ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg
    20221 ctgtatgaac aaaaagaaat caaaacagcc gcaaaagaaa tcatgttacg tgatggaaaa
    20281 aaagaaaatg aattctcatt aacaattttt tgaaggagcg aatgtatatg aaagttaaaa
    20341 aagcagttat tccagcagca ggattaggaa caagattttt accagcaaca aaagctatgg
    20401 caaaggaaat gttgccaatt gttgataaac caacaatcca gttcatcgtt gaagaagcgt
    20461 tgaaatcagg tatcgaagac atcttgatcg ttacaggaaa agctaaacga ccaatcgaag
    20521 atcactttga ttcaaatatt gaattagaaa caaacctaag tgaaaaagga aagaccgaat
    20581 tattgaaatt agtagaagaa acgacagatg tcaacttgca tttcatccgt caatctcatc
    20641 caaagggttt aggacatgcc gttcttcaag caagagcctt tattggaaat gaaccatttg
    20701 tcgttatgct aggcgatgat ataatggaag acgaagtacc attgacacag caattaatcg
    20761 atgattacga acgtactcat gcttctacga tcgccgttat gcaagttcct catgatgaaa
    20821 cttcaaaata cggaattatc aatccaggag aagtattgga agatgggtta tacaatgtca
    20881 aaaactttgt tgaaaaacca gatccaagca aagcaccaag tgatttagct attattggac
    20941 gttacttatt gactccagaa atttttgatg tattgtccga gcaacaacca ggagccggaa
    21001 atgaaatcca actaactgac gcaatcgata cattgaataa aacacaacgc gtatttgccc
    21061 gtaaattcac aggtaaacgt tacgatgtcg gtgacaaatt tggttttatg aaaacaagta
    21121 ttgaatatgg tctaacacat ccagaagtag gtgcaccttt acgtgagtac atcctgtctt
    21181 taggtaaaac attagaagca gaaggaagca agatcgatca taatccaaaa ggaataacag
    21241 aaatcaaaga agaaaagaaa aaataaccct tttttctaac gagcttcagc tgctaattga
    21301 ataaaaagta gttgaagctc gtttttattt gtttaaaaaa cacgaaaatg ctatccttat
    21361 agaaaagaga gacaagaagg gagtttgatc atgtcaggag gagaaattgc aggattgatc
    21421 gcagcaatcg cctttgcgat acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct
    21481 aaaactttag aaaaagtaga tcagactgta gcagaagcaa atacgacgat cgaaatcgtc
    21541 acaaaagacg tagatctttt gtctagacaa gtagaaggct tactggtcaa aagtaatgaa
    21601 ctgctcttgg atatcaatgg aaaagtcgca acgatcgatc cattgttcac agcagttgct
    21661 gatttaagtg aaagtgtatc agaacttaat cattctagta aaaacatcgc gacgaaagtc
    21721 ggcggaatcg gtaaaacgac agctaaagcg acggttgccg gaaaagtcgg tgggacagcg
    21781 atgaaatttt ttaaaaacaa aaaacatact gaaacgacag gaggaaaata aaaatggcta
    21841 aaaaaggtgg atttttatta ggtgcagtag tcggaggcac agccgcagca atcgccgcgt
    21901 tattgttagc accaaaatct ggtaaagaac ttcgggatga attagctgcc caagcggatg
    21961 atttgatgga tatggcttca gattacacag atatggcgaa agaaaaatcg actgagttaa
    22021 caggtattgc aaaagaaaaa gcgcaagatc tttctcaaca agcaggtgat ctagcaggta
    22081 gtgtcaaaca aaaagtaagt gaatcagcag atgaagcagg aagtgaatct gacgatatct
    22141 tgttaaatct taaagaacaa tcagctgact tatctaatcg tttcaaaaaa acggcagaag
    22201 atatgaaaaa acctgcagat gaattaggaa gtattgctaa agatacttca gaagatatca
    22261 tgattgatgt aaaagacgct gctggtaaag caggagatac tgtcaatgaa ggaatgtcag
    22321 aagcaaaacc agttgcagat gactcaaaag aaatgatgaa aatggcaaaa gaagatgcga
    22381 aagaagctaa aaaagaaata aaagatacaa tgacagattc atctacaaat cactaaaatc
    22441 acaaatttat agaataaaaa ggtgggagcg gactagctct cacctttttt actcttattt
    22501 ttctaaaaca agtaattctt ttggtgtagt cgtgaatggc acacagccat cgcttgtcac
    22561 atatacacag tcttcgatac gtacccctac tttttcagga atatagattc ctggttcaag
    22621 agagaagcac atcccttctt cgattactaa gtcatttcct tctacaaggg aagggaattc
    22681 gtggacggtt gttccgatcc cgtgacctaa acggtgattg aaatattcgc cgtagccata
    22741 gctgttgatc acgtttcgtg cgatttgatc aagttcacct gctgtcacac ctggacggac
    22801 ggcttcttgt gcggcaagct gtgcttcaag agtgatgttg tagatttttt cttggaagtc
    22861 ggttggtttt tgataagcaa ttgtacgagt agcatcactg caatagccat tccaaacaac
    22921 gccaagatcg aataacacaa gttcattcgg tgaaatcttc gtatttcctg gtgtaccatg
    22981 agggcttgca gcatttgctc ctgcaaggac taatgtgtcg aaagacattt gtgaaacacc
    23041 ttgttttttc aattggtatt caatttctgc aatgatcgct tgttcagtca cgccttcttt
    23101 cactgcttta aagccaatct caaatgcaac atcggcccat ttaccagctt cgatcagttt
    23161 gtctttttcc tctagagttt tgatcagttg tagacgttgg atgatggaag ttagatcagt
    23221 gtaagaatcc gttcccgtca agatatcaga gatagcatcg aagcgctcga cagtcaaggc
    23281 gttcttttca atccctacat tgccaggctg accatagcga gcacgaagtt cttttccgat
    23341 gatctcccaa gggttttcgc tgtctaaata gccacggaca tcatacgccc aactactgtt
    23401 ttgagcatct tcaacttcca aagcaggagt gaaaaggaaa ggatcttgtt caagagggat
    23461 aaaaagagct agtacacgct catgaggatc actttcgtat ccggaaaagt aagcgatgtg
    23521 gccagggtcg ctgatataag ccaattcgac ttggtgttgt gccatccatt gacgtagttc
    23581 attgattttt acgttattca tttggcaacc tactttctaa attaattttg tattcaataa
    23641 ttgtaccact gatttattaa aagaccaacg ttctaaaaac tgaaaaaagt aaaaattttc
    23701 atgaaaacag agataaaacg gttgcaattc ttgagattat ttgatattct attttagaaa
    23761 tgtaagacgt attttcagaa aacatataaa atatgaatag gagaacacaa atggaaaagc
    23821 aaacaatcac tatctatgat gtagcgagag aggcaaatgt ttcgatggct actgtttcac
    23881 gtgtcgtaaa tggcaatcca aacgttaaac cagcaacaag aaaaaaagtc ttggaagtta
    23941 tcgatcgctt ggattatcgt ccaaacgcag ttgcgcgtgg cttggcaagt aagaaaacaa
    24001 caacagttgg tgtaatcatc cctgatgtca gcaatatgtt ctttgcttct ttagcacgtg
    24061 gtatcgatga tgtggcaaca atgtacaaat acaatattat cttagcaaat tcagatggca
    24121 acgaccaaaa agaagtcaat gtattgaata acttactggc taagcaagtg gacggcgtga
    24181 tcttcatggg tcaccatatc acagacgaaa tccgtggaga attttcacgt tcaaaaactc
    24241 ctgttgtatt agctggctct attgatccag atgaacaagt aggtagcgta aacattgatt
    24301 atgtatccgc aacaaaagat gcagtaaaca aattggcaaa aagcggcaat aaaaaaatcg
    24361 cttttgtcag tggtgcgttg atcgatccca tcaatggaca aaatcgtctg aaaggctata
    24421 aagaagcatt aagcgaaaat aacctaccat atagcgaagg attgatcttt gaagcacaat
    24481 acaacttcaa agatggtcta tctctagtcg atcggatcca taatagcggt gcaacagctg
    24541 tctatgtttc agatgatgaa ttagccatcg gtattttgga tggtttatta gaccgtggcg
    24601 tgaaagtacc ggaagaattc gaaatcatca caagtaacaa ttcactactg acagaagtag
    24661 ctcgtcctcg tttaacaagt atcacacaac cattatacga tttaggggcg gtatctatgc
    24721 gcctattgac gaaaatgatg aataaagaag aagtcgaaga aaaaacgatt atcttgcctt
    24781 atggaatcga agaaaaagga tctacaaaat aataaaagga caaccattca gaggttgtgt
    24841 gcctgccgtt taactctgcg ttacaaggag caatttcaaa aaacagcatc tcatgtttga
    24901 tgaatattgt gactcgtgat tcggagttgg caggagaaca ccgtttattt gggaaaaggg
    24961 gttatgacaa aattttgtta taatcctttt tttgtgtctt aaaaaagaga gaaagtggtt
    25021 gaaattacat ttatcgtata taaaacaatc cgtgtataga tgctgcattt tgttcaccac
    25081 catcaaaaaa ataagggact gtgacaatac tattgtcaca atcccttatt actgtgtatt
    25141 ctattaaatc aatcatcttt ctcatctgat tctttagttg cttgattcgc tcttgcataa
    25201 gctgaagcgg tgttccagta atctgtatag ttttcatcgg taccgccaac gccaaattta
    25261 aaggcgcttt tcttcggtcc atttttcgcc catagcgatt caactgtttt tcctggtgtt
    25321 tggatggatt ttttattgac agtaactttt ccaggtgttt gaccagtaaa tccagatact
    25381 tttttcttca tcacatcaga tgataattcg aatttctcat cagtgccaaa aatgtcaggt
    25441 tttgcttggt aagcttgatt gatcagattg gcaagataat tagccgaacg tatccttgct
    25501 ttgctgtcca ttccagtatt gtcatcgtgc cctgcccagc tactgatggt aatagacgga
    25561 gtagagacaa ttagccatga atctcggtat tcattggtag aaccagtttt tccgacccaa
    25621 tctgcttttc ctagatttcc gtttaagctt gagatggcat ctttgaatgg tgtagtgatt
    25681 tttgcattga tcacactacg catcatgtca ttcatgatcg atgccgttgc ttccgaatag
    25741 atccggacag agttgctttc gtgtttataa attacatttc ctgcgttatc tgtgatggaa
    25801 tcgatgatat agccttcttc atagacgccg ccgtttgcta aagcctgaaa tccgttggtc
    25861 tgttgaagcg tcgtgacatc tactgtacct aaaggagcag attcaacgcc ccaattgtca
    25921 tttgccgggt agttcatctt tgccaagtag tgctcgtaag catattgttt cgatccaccg
    25981 gaattcaaca catcttgata taagtgataa gccgggatat tgttggacca ttctagtgac
    26041 tcacggacgg tttggaaggt gttcgatcct tcgttagtag cattgacgat tttctcgcca
    26101 gcattttctc cttcttgcca agttgttgca taatctgata cacgggattc tgatccgata
    26161 agccccatgt cgattgcagg tccataaaca agaactggtt tgattgatga gccagcttga
    26221 cgttctgtat cgaaggcatg gttattttga ttctgactgt aattccgtcc gccaacgaat
    26281 ccgtagatgc ggccagttct gttatccatc aatacatttc cggtttctac ttgactgcca
    26341 gcaccatcat ctaacatgta tccataattt tcaacgccat tttgcaatgc agcatagaca
    26401 tctttatcga ttgtcgaatg gattgtatat cctttatttt ggatcgtttg ctgtgccttt
    26461 tgataataag cgtcactgac cgatgaatca gacatatcag cgttgtcttt ttcagccaat
    26521 tgttgtgcaa tgattcttgt tgcttcattc atcacagtat aatacaagaa ttctctgtca
    26581 gtctgttctg cgatttgttg tggtagaaag tcttttgtaa ggtcataggc ttttgcttct
    26641 tcgtattgtt ctttcgtaat ttggccttca cgatacatac tgaataaaac aaaatctttt
    26701 ctggcaaggc cggcagaaag atcttctttt agcgcgcctg tgttcgtgta tggactatac
    26761 gtgatcgggc tttggggtaa tccagcgata taagcagctt gcggcagcgt cacatctttt
    26821 gcatctacac caaagattcc ttttgcagct tcctggactc cagcaatgtt ttgtccctta
    26881 ttattacgac caaatggtga gacattcaaa tacgtagcaa tgatctcatc ttttgaaaag
    26941 tatttttcta cttgtgcaga aagaatgatc tcgtttgctt tacgtttgaa tgttgtttca
    27001 tctgtaagga tctgctgttt gactaattgc tgtgtcaaag ttgatccacc agaagagcca
    27061 ataccagtag cttcagatac taatgctcgt aaaacagctt taggaacgta accttggtgt
    27121 ttatcgaagt attcatcctc tgtgctgata attgcagttt tcaataaagg tgaaatctta
    27181 tcagatgaaa tagtcgtgcg catcagatct gtctggattt ttgatatctc tgtattatct
    27241 gcataaacaa tctttgacgt ttctgtaatg tttcctagtt ctgtctgcag tgctttttta
    27301 gtcggcaatt gtgtgtcttc gacgagatag gcaaaatagc cagcgcctat tccaatacct
    27361 agagctgcga acatcaaaag aagaacagta ataaaaacag tcaatgattg aaaaactcta
    27421 aaaataatct taggaacaaa ccatttttct ttttttcttc tattctgttt ttttcgagta
    27481 tttttgcgtg gcaaaagggt cacctcaact aatggtttta tttcgctaat tatagcaaat
    27541 aatagaacaa aaaaacagac aaggctgttt ttggcaggaa tttctttttt aaaagagtta
    27601 aaacgtgaga aaatgatctt tattataaaa gagttcttaa gcttttcttt agagaaaatt
    27661 tcagttacta tggagaacag ggcaaagaaa aatcaatcat tttctttgta tatcatctta
    27721 ccaatatata ggaattgtta gtaacaattg cctttcaagg aggaatccat ccatgttatt
    27781 aggctcacac gtcagcatga gcggaaagaa aatgctgtta ggagcagcag aagaagcagc
    27841 cagttatgga tcttcgactt ttatgatcta tacaggcgcc cctcaaaaca ctagaagaaa
    27901 agccatcgaa gagatgaata ttgatgccgg aaaagcgtac atggcagaaa aaggtctaac
    27961 aaatatcgtc gttcatgccc cttatatcat taatttgggg aatacgatca aaccagaaat
    28021 gtttcctttt gccgtccaat ttctgagaga agagattgtt cgtgcggaag ctttaggcgc
    28081 tactcaaatt acacttcacc caggtgctca tgtaggggca ggaacagaag ccggaattgc
    28141 tagaattatt gaaggcttga atgaagtttt gacaaaagaa cagacggcac aaatcgcttt
    28201 ggagacaatg gcaggtaaag gtacggaaat cggtcgaaca tttgaggaat tagcgcaaat
    28261 catcgatgga gtaaaattga atgaaaaatt atccgttact tttgacacct gtcatacgaa
    28321 tgatgccggt tataatatta aagaagactt ttcaggtgta atggaagagt ttgataagat
    28381 catcgggtta gatcggctta aagtactcca tatcaatgat tctaaaaatc cgcaaggttc
    28441 gcataaagac cggcacgcca atattggttt tggaacaatc ggattcgaca cgctgaataa
    28501 gatcgtccat gatccaatgt ttgacaatat ttcgaaaatc ttggaaactc cctatgtagg
    28561 caaagataaa aagaaagctt acgcaccata tggaaaagaa atcgccatgt tcaaagcgca
    28621 aacattcgat ccagaagtgt ttcctgagtt attagcagaa gaagtgatac tctaagattt
    28681 tctatctgac agcagcaaaa aaggaccagg cacattgcct tgtccttttt tgtttaagaa
    28741 acaaaggagt actttttgca gtctttttca tcatgcatct tctcccccct ctttttaaat
    28801 ttagacaaac aaggtatact ggtagaaaag taaaaaaacg aaaagtttca caagcaagtg
    28861 gcaaaacgtg attggaagga cgaaaagatg ctgaacactt taaaaccctt gatcaaccaa
    28921 ctaaaaaaga aacatacata ctttattctt ggcatagccc tattatttac aattattcgt
    28981 ttgtatttat atagtaaagc aatctatgga acagacatgt cggcaaccta tgatgaccag
    29041 ctgctgattc gctatgctca aaatattttg gacggtaaat ggctagggga gtatacgact
    29101 acaacacttt ccaaaggaat ctcctacagc ttatttttag tacttgcaca taaactgcat
    29161 ctttcttatg cagttttgtt cggcatgttc agtatttttg ccagtatatt ggttgcactc
    29221 gcgtttaaac cgattgtgaa gaacaagtat cttcttttaa gtatctattt ttattttttg
    29281 ttttctccga ttagttttac acatgaatat tccactcgaa catatcgtaa tgccctcgtg
    29341 attccggctg ttgagatcat tatcgcttgt ttattagcta tttattaccg caaatacggt
    29401 tctagcagaa aattgctgcc atggttttta gggctttctg tcgcttttcc tttcttttgg
    29461 ttcattcgcg aagattctat ctggcttctt ccttttactg tagtagcttt gggtatcagt
    29521 ttcgtccaga tcttgctagg gcaagacttt tctttcaaag aagtaaagaa gttaaaaaaa
    29581 cgactctttc ttctctcgaa acgggaatgg ctgaaacttt cattgttttt acttcctata
    29641 gttggctttt ccatcgtcta tcaatggatc gaatataaaa atgagacaac atacggtatt
    29701 gcaaccatca atgaccgttc cagtggagaa tttggacgtt tgatgaaaca actgatccga
    29761 atagatgatg aaacgaactt gaataaagaa aacagtgaga tatgggtctc acatgctgca
    29821 ctaaataaag cattagccgc atctcctaca ttccatactg tttctacccg tattgacgaa
    29881 ttatatacga ctcacgcgtg gactcaaggt ggaaaagtaa aagaattacc cggagatatc
    29941 atcttttggg cattgcgaga tgtggtaagc gaatctggtt attaccaaaa caatgcaaaa
    30001 gaaaccaatg atttttggaa aaaagtcaac caagaattag aagaagccta tcacaaggga
    30061 acaatcaaaa agaaaaaaga gttctatctg atgggatcag gagatggaaa gacgatgagt
    30121 gatctgccaa tcgtattcga tttccttcaa tcaggatacg cttatgcatg gcgatataaa
    30181 gatttccatc aaggaggaaa ttatagtttc ggaacgcaag aacaagtcaa acaggctcaa
    30241 gaattattaa accttccgct aatggagaat tggatcgata gtaatgaacc gagaacacct
    30301 aatttccaat taacaaaatc agcaaaagtc gcgaatgtca tcataaaaat ttaccaagtt
    30361 ctggctattc ccgtgctgat tgcttcaata gcagggatat tacttatgat actaggaacc
    30421 gtatttgcaa aagctgatcg tgcctattat agttcctctt tgattgtggt caccggatta
    30481 atactgacac aattggtttt tcttttcggt gtctcatggt tttgttctta tgcgccagaa
    30541 aaaaaggact attttttaag tgtctacact ggtgcaggcg tgccattaac gcaattcatc
    30601 gtagtaacaa gcatgctagt tttcagtaaa ttaaatctga aaaaagcttt atcttctaaa
    30661 taaaaagacc cttggtagct agatttttaa cctaactatc aagggtcttt gtgtggaaaa
    30721 atcagttttt attcgatttc ttctaccgtt tcctgcaaaa gctgttcata ttttttgttt
    30781 cctttatata aatcaccaat cgtccatgcc aacaggagaa ggacaagaac aatgattaca
    30841 taagcgatgt gatcagccaa tgctagttga gcggaagtgg cttgctcgcc gaagaaactt
    30901 tcgatctgtc ctggtaatcc tcgtaagttc aagtaagtta gactaatcac agagaaccag
    30961 ccaagaagtt ttacgatgaa tgaattttta aatcggctac ccatctccgc ttggctgttg
    31021 gtcatcatca gtaaaggaat catcgaaaac ggcaacgcaa aagccaagaa cacctgggat
    31081 tcattcatta agttattcaa agcggcatgt tcttcgatcg ttccttttgt acttgtaaac
    31141 aaaacacaaa tcaatacagg aatcacagaa agcaatcgtg tgaccagtct tcttagccaa
    31201 atcgggatac gcatatggat gaatccttcc atgatgactt gaccggtcaa cgtaccagta
    31261 atagtagagt tctggccaga agcaagaagt gctacagcaa ataaagtgga caagatacca
    31321 gattttgcta cagcaatcaa aataccattg ctcatagctg atggatcaga taaagcttca
    31381 tacagaccaa aaaaggaagg atcttggaca gaaccggttt tgaagacagc gacacccatg
    31441 atcaatagca gtgcattgac tagaaaagcc attgacaact ggatattaga atcccaagta
    31501 gaaaaacgta cagcttgtgc tacatcttct tcgttttggt gatcgatctt ccgagtttgt
    31561 gaaactgctg aatgaagata taagttatgc ggcatgactg ttgcaccaat aatccctaaa
    31621 gctccagtca gcggagtttc gccgccgatg accggtgagt ctgagaatgt atgactgtta
    31681 gggataaagc cagcaaataa actgtgccaa tctgggttag acaagccaac ttgatagaca
    31741 aaaacgaata aaatgacgaa aattaagcag acaaccagtg cttcgatctt acgaaacccg
    31801 acttttgtca gtagtaagag aacgaaaaca tctaatacag taataaagac ggcaatcacc
    31861 aatggaatat caaaaagtaa gtagagggcg attgctgcac caatgacttc tgcaatatct
    31921 gttgccataa tggctaattc agtcaagatc cataaaatga cacctaatgt cttacttgtg
    31981 cgtgctcgga ttgcctgtgc caaatccatt tggctcacga ttcctaattt tgctgccata
    32041 tactgtaaaa gcatagcaat cagactagac atcaaaataa ctgacatcaa taaatattgg
    32101 aaattttgtc ctccggtgat cgaagtagac cagttacccg ggtccatata cccaactgct
    32161 actaacgcac caggtccaga gtaagcaaga agtgttttaa aaaaactttt cccctttggt
    32221 acatcaactg ttccatttac ttcagctaaa gataaaccat ttgtatgttc aataagtcga
    32281 tgcttatgaa attgtttatt ggattgatta ttcaagaaaa aaccaccttt cctatttatc
    32341 tacgattaca ttctacacct caaaaagaaa aaattaaaga aaaagattcg gtacgcctaa
    32401 ttttttttca tggcgcgtct tgctagatga cgaaagaaat tttgttctcc tctaaaaaaa
    32461 ccactatttt ttgcatgaaa ctaaagaata cgcctttctt ttttcaataa actttagtaa
    32521 aataagtcat tgagagcaat tcggaaatag aaaggagaga tttatatgtt atcaacaggt
    32581 attgtcgtac ttattgctat tattgctttg ctaatcggcg cagtaggtgg ctttttccta
    32641 gcgcgtaaat atatgaaaga ctatttagaa aaaaatcctc ccgttaatga ggacatgttg
    32701 cgatcaatga tgatgtcaat ggggcaaaaa ccatctgaga agaaaattcg ccagatgatg
    32761 cagcaaatga agaaccaagg gaaaaaatag gttctgaccc aacgactttt tacgggctga
    32821 ttttttcagt ccgtttttta tttcgtaatt tgttagacta ttcagattat tcagcaaaaa
    32881 agacaagcac agacaatcct aggctcttga taaagacaaa ggaggagtaa aatgtcgatt
    32941 tttagaaaat taggctggtt ttttcgtcag gaaaagaaac attatttaat tggggttttc
    33001 tcgctattcg cagtggcctt ggtacagtta gttcctccaa aagtgattgg gatcatcatt
    33061 gatgagatcg ccgataaaaa tttatcaata aagatcattt tcgggtggat cttcctattg
    33121 attatcgctg cttttttaca atatcttttt cgttatatct ggcggacgaa tatttgggga
    33181 agtgcagctc gattggagaa agaacttcgg cagcagttat tcaaacattt tacagaaatg
    33241 gacaatctct tttaccaaaa atatcggact ggggatttga tggctcacgc aacgaatgat
    33301 ttgaatgcga tccagaatgt agcaggagct ggtatcttga cctttgcaga ttcagtgatt
    33361 acgggcggaa caacgatcat tgcgatgatt ttgtttattg attggcgctt aacactgatt
    33421 gcgctgattc ctctgccttt gctggccgtc acttcacgag tgctggggtc aaaactccat
    33481 gatgcttttc gtgattcgca agaagctttt tctagtatca atgataagac acaagaaagt
    33541 atcactggga tcaaagtcat taaaacattt ggacaagaaa aagaagatct agcggatttt
    33601 gaagaaaaga ttgaagatgc aattattaaa aacaaacgag tcaatttttt agactcctta
    33661 tttgatccat tcattacctt gattatcggt ctgtcttatg ttgcaacaat tattttaggt
    33721 ggaaagtatg tcttggaagg cacgatctcg atcgggcaga tggtctcatt tattagttat
    33781 atcggtatgc ttgtttggcc gatgtttgcg attggtcgat tgttcaacgt attggaaaga
    33841 ggaaatgctt cttatgatcg cgtcaatgaa cttttgcatg aaaagacgca tatcgtggaa
    33901 caactgggtg cagtgcagac cccggcaaaa ggcgagctga atgtagctat ttcctctttt
    33961 aattatccga aagaagaatc accagcatta gaacaaatca agtttgcgat ccaagaagga
    34021 gaaacattag gtattgttgg gaaaactggt gcgggaaaaa cgacgatcat caaattactg
    34081 atgcgtgaat acgatgatta tcaagggaaa atccaatttg gcggacgttc gatcaaagaa
    34141 tattcattga atgctttatt aggatcaatc ggctatgtgc ctcaggatca cttcttgttt
    34201 tcgatgacca ttcgagataa tatccgtttt tctaatccaa aactttcaga agaagaagta
    34261 gagcatgcgg cttctttagc atttatcaac aatgaaatca aggaattacc aaaaggttat
    34321 gataccttag ttggagaacg tggagtctct ttatctggcg gacagaaaca aaggatttcg
    34381 attgctcgag cattgatcaa aaatcctgaa ttattaatat tagatgatgc actctctgca
    34441 gtagatgcca aaacagaaga agctatcttg cataatttaa aagaaataag aaaagagaaa
    34501 acaactatca ttgcagctca ccgtttaagt agcgtgatgc atgcaaaaga aatcattgtt
    34561 gtcgatcaag ggaaaatcat tgaacgagga acccatcatg agctgcttgc actaggcggc
    34621 tggtatgaac aaatgtggga gaaacagcaa ttagaagcga agatagaagg aggcgaggcc
    34681 tgatggatga aaattatcaa tcggaatgga caaaatcgat tccacttaaa gaacagaaaa
    34741 agatcatcaa acgactgatg aagttcgcca aaccttttag aagaacgttc atcgttgcta
    34801 tcttatttgc gtttgctttg tctgtgatca atgtcctatt gccaaggatc atccaaactt
    34861 ttatggatga tcatctggct aaacagtcgg ctacgacaca agttattttg ttttttgctg
    34921 gtatgtactt atttggagtc atagtaaaaa gtatcatttg gtttttccaa tggtatttgt
    34981 attccatggc ctcattgaag acctatcagt atattagggt aaaattattt gaaaagctgc
    35041 atacactagg tatgcgttat tttgatcaga caccggcagg atcaaccgtt tctcgagtga
    35101 cgaatgacac agaaacgcta tttgaatttt ggtacgtctt tttgatggta ttgactggga
    35161 tctttgcagt cgtttcttca ttccttgcca tgtttcaaat caatggagaa attgccttgt
    35221 ggactttggt gtttttgccg attttaggaa tcgtcatctg gtattaccag aaattcagtt
    35281 ctcgtattta ccgaaatatg cgggaaaaat taagtcagct gaacacgaaa ttgaatgaat
    35341 cgatttccgg catgcgtatt atccagcagt ttcgccaaga agctcgttta gctaaagaat
    35401 ttgaacaagt gaacaatgag tatttagaca cgcgttatgc gatgatcaaa acgaattcga
    35461 ttctgttaag tccgatcatc aattttctat atgcattggc gattgcgttg gtgttgactt
    35521 tgtttgggat cgatgcgttg cattctcccg tagaagttgg actgatctat gcatttacga
    35581 cctatgtcca gggatttttt aatccaatgt ctcaaatgat ggactttttg agcatcttca
    35641 cagatgggat ggttgctggt agtcgtatct tgaagattat ggatacggaa gaatatgcac
    35701 cacaacaaaa ccctggagca gatcaaatga ttacttcagg taagattgag tttcgcaatg
    35761 taagcttttc ttatgatgga gagcacaatg tattaaacaa tatctctttt gttgctaatc
    35821 cgggagagac cgttgcatta gtgggacaca ccggaagcgg aaaaagctcg atcatcaacg
    35881 tattgatgcg gttttacgaa ttttatgaag gtcagatatt gattgatgat catgacattc
    35941 gagaatatcc aatggaagag ttacgtaaga aaatggggct ggtcttgcaa gatgctttct
    36001 tgttttatgg ggatatcgca ggaaatattc ggatgatgaa tccagctatc acagacgaac
    36061 agatacgcca tgcggctgag tttgttcaag caaacaaatt catcgatgaa ttaccaaaag
    36121 gatatcactc aaaagtgatc gaacatggtg ccagctattc tagtggacaa cgacagctga
    36181 tcacttttgc gcgaacaatc gtgactgatc ctaaaattct agtattggat gaagcaacag
    36241 caaatatcga tactgaaaca gaaggattga tccaagaagg attggcaaaa atgcgtcaag
    36301 gacggacaac gattgcgatt gcccatcggc tgtcaacgat tcgagatgca gaattgattt
    36361 tagttttaga caaaggtcat atagtagaac gcgggaatca tgaaacactt ctagcgcaaa
    36421 atggtttgta tgcagatatg tacaaactgc aaagcagtgg tatataaaag atgttgtgac
    36481 agtagaattt tgacctaagc actagagtga aaataaaaca tagcttctca tatttttcat
    36541 ctgtgagagt tagtgtcgta gtcaccagat ttaaaaaaag attcgacagc tagtcagaag
    36601 tgcctaatag ccactaagaa agcgttgaca aaagcatttg ctttcgataa tataaagaaa
    36661 acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt ctcccatggt
    36721 ttcatggacg gctttttggg tttttctttt tgttttggat acagccctat acatcatgga
    36781 aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat aaccagggat
    36841 gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa gaaagtgggt
    36901 gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga cgggctttca
    36961 caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat ttcttgtgaa
    37021 atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt gtatattgca
    37081 ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt tgtaccaaat
    37141 gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg gaaaaaaaca
    37201 gtgaagaaaa tctcggataa ataccacatc aagttatcag aagatgaagc cggatttatc
    37261 gcattgcatt tagtcaatgc acaaggcatt caaagccagc aagacaatac ctacctgttt
    37321 actataacaa tacaggatat tctaaatatc gtccgctatt actataagat tacatttgat
    37381 gaagattcag tccatttttt tcgattcgtc acacacttac gtttttttct gacacgcgtc
    37441 ctagatggag aaaaatcaga agtgggagaa gtttctgagg aattgtttca ggttattcaa
    37501 aaaaaatata aggatacgtt tggttgcgtt gagaaaatag cagacttttt agccgagacg
    37561 ttcaactacc agatgtccac ggacgaaaaa atgtatttaa cgatccatac tgcgcgttta
    37621 gtgcatgaga atcgttgtaa ataaaaaaag gaggataaat taacaagaac gtatgaaata
    37681 agggtagtga cattcagttg gctaaacctt ccactaataa attggaggga atagaaatgg
    37741 gaaaatacca agaattagcg agaaaaatcg tagaaaatgt cggcgggaag gaaaatatca
    37801 atggtttgac acattgtatc acaaggcttc gtttcaaatt aaaaaacgaa gaaaaagcaa
    37861 atgatgagat cttgaaaaat atggacggta ttgtaacggt aatgcgagcg ggagggcagt
    37921 atcaagttgt cattgggaat catgtgccag tcgtgtttga agaggttata aaagcaggaa
    37981 accttacttt tgatgaggct gtttcaacag aaaaaatgag accgtttgat atgcttattg
    38041 atggaatatc gggctgtttc cagccattcc ttgctatcct agctgctggc gggatgattc
    38101 gtggactgac agcattcctt gtcttcttag gcgcgtttga ccgcggttct ggcacatttg
    38161 ttatgtttga taatatcggt gattctgtat tccaatttat gcctgttatt atcggtttaa
    38221 cagctgcccg taaatttaaa gtaaatgaat tcgtagggat gttgcttggc gcagcattaa
    38281 tgaatcctag cttgtcgctg gaagctttga gtggtgcagc agaagcacca ctaacgacca
    38341 tattttctgg cactattttt gaagcaccta tttatcaaac agtattcggc attccctgga
    38401 ttgcgcgtaa ctacgcttct tctgtcattc ccattatctt tattgtgttg ttagctagcc
    38461 aaattcagaa accaattaaa aagttagtac ctgagatgat cgcgaatttc ttcgttccat
    38521 tcttcaccgt tcttattaca atgcctttag gttttttact cgttggacca gtattcacat
    38581 tcgcaactga tattttaatg gctggatttg aaacactttt agcactttcg ccagttattt
    38641 atggagcaat cgtaggcttc ttctggcaaa ttctggtgat gtttggtctc cattgggcaa
    38701 tcgtaccaat gggattgatg caattttctg tgaatggttg gcaaaatatt atgacacctg
    38761 ttgcagtagt gtcttttgga caaactgcag cacttaccgc actctacttt aagcttagaa
    38821 atccaaaaga caaagcaatt gcaataccag ccattgtttc tggtattgtg gggattacag
    38881 agccagctat ttatgggttt acattaccac gtaaaaaaat atttattttt acctgtgtcg
    38941 gtggtgcgtt agggggcgcg tatagtgggt taatgaattt aacttcttgg aatcaaggtg
    39001 gactaggcat ctttacaatt cccaactata ttagaccaga tggtgattta acagatgtga
    39061 ttaatgtatt gattgggatc gccattgcga tggtggtatc ctttacgctg acttttttct
    39121 tttggaagga tgaagcaggt gaaacagatg atatacagaa aaaatcaggt aaagaaattg
    39181 taaaaacacc gatacaagga caaatcgctc cactaaatgc agcaaaggat gcagcttttg
    39241 cacaaggaac actaggtcga ggaatcttga tctaccctga aaaaggagaa gtacgtgccc
    39301 cttttgacgg aacagtcatg accctgttcc cgacgaaaca ggcaattgga atggtttctg
    39361 aaacagggct ggaattgctg attcatgtcg gattggatac tgtgcagttg gaagggaaat
    39421 attttgaaag cttagtgcag caaggataaa gagtgaaaca aggcgatctg ttgctacgct
    39481 tcgacaaaaa gaaaatcgaa aaggaaggat acagtttaga aacacctgtg atcgtcacca
    39541 attacacgga ttatttggat attttggaaa accaagggga gacggtcggg ccagacgatg
    39601 cacttatcac cgcactgacc tgaacggtta ggtgaagaat ggactaggct taactattct
    39661 ttttattcca cttctttatt ttcaaaattg aaaggctctt tcatgatggc tgagctgata
    39721 gggcgtttat cgaatttttg accgacagaa attagatgga ctaaattctg ttgataattt
    39781 tctgcaaaaa cacaactata taacacgtct agaagataac aaattgatgt attgatcgta
    39841 aaattaccaa tttttgaata cagtcgctcg cgtgtcgtaa gtcgcaaagc ggctgtactc
    39901 attgcagata gcgttgaatc accgatactg gtcaaagcta aaattggaac atgattttgt
    39961 ttcaatattt ctgcggtacg aaggatcaca ttgttttcac ccgtataaga aatcagaatc
    40021 ccgcacatat cactcgtgca attgtacgct tcatagaatg attcaccaaa agtggtgaca
    40081 atcgtgacat ccattttgat gcgcttcatt ttcagcatga aatcttgaga aatcaaagta
    40141 ttcgagttac tggcgaaaat cacgattttt tttgcttgaa gcattagttg tttggctttt
    40201 tgcagttggt catgatgaat gagagaaagt gtatcatgaa tcgtcatctg ttctaaagaa
    40261 gccattttat ttgcgatcgt cataataccg tctgtggtgg aaaaaggaag attcgcatct
    40321 acgtcttgaa aattcccttc aagataggct gtttcagcta aaaaagcatt acgaaaactg
    40381 acccagccat caaaacccat tttttttgct acacggatga gtgtagaggg gtggacataa
    40441 catcgctcag caatcgcttg gatggtcatt tcgccaagtg ctggagaatc gcttaagaga
    40501 aactggatca cttcattttc tgaaggagaa aaggagattt ctttgatttt ttcacgtaat
    40561 aacatgtaga taccttcttc ttattttttt acgtaaatta taactttttt gaaaatgatt
    40621 tgtaaacgga tttatactat ataacttaaa atagacattt tttgacaaga tatttacaaa
    40681 aatccactag aaaacagtaa attttgtaaa tgttactgga aagaaacaga tttctattgt
    40741 ttgttttgtt tacaaaaaag ataatgaaag tgtaaaaaag taaaggagtg agaacatggc
    40801 aaaaggaatc aaaatcgcta cgattggtgg aggcagcagt tacacccccg agttgatgga
    40861 aggatttatc aaaagatatg aggaattacc gatacgagaa atctggctcg tagatgtaga
    40921 agaaggaaaa gaaaagcttg agatcgttag tcagatgtct cagcgtatgt gggatgcttc
    40981 tccgtatgaa gtcaagatct atgcaactct ggatcgggaa gaagcattga aagatgcgga
    41041 ttttgttacg actcagtttc gtgtcggtca attagatgca cgaatcaaag acgagcggat
    41101 ccctagttat tatgggatga ttgggcaaga aacaaatggt gctggcggga tattcaaagc
    41161 ttttcgtacc gttccagtcg ttttggcaat cgttgaggac atgaagcgtt tatgtccaaa
    41221 tgcttggctg atcaatttcg cgaacccaag tgggatggtg acagaagcag ttatcagata
    41281 tggcaaatgg gataaagtga ttggattatg caatgtgcca gtgatggcga tgatgacaga
    41341 accagcaatg cttggcaaga aaaaagaaga gctgatctac aagtttgccg gtctaaatca
    41401 tttccattgg cacaaagtag cagataataa aggaaatgac cgcacggcag aattgattga
    41461 tcgtttatac gatgaagaga atggattacc gaaaaatatc tttgatatcc cgtttttcaa
    41521 agaacaatta aaacaaatga acatgatccc ttgtggctac caccgctatt actatcgtga
    41581 agaagaaatg ctggaacatg ctttagaaga atatcaaacg attggtacaa gagcgcagca
    41641 agtgaaagaa acagaagcag agctatttga attatacaaa gatccagaat tagaccataa
    41701 acctgaacaa ttgcaaaaac gaggtggcgc ttattattca gacgctgcct gtgaaacgat
    41761 tgcttcaatc tatggaaata aagaaacaca gatcgtggta tctacaaaga atgatggagc
    41821 tgttcctgac ctgccggcag attgtgtagt agaagtcact gcctatgttg gcgcacaagg
    41881 agcgagaaat gtagcgtttg gttctcttcc tgctgctgaa agaggctggt tgcaagtaat
    41941 gaagaatatg gaattgctga cgatcgaagc agctgtgact ggtgattatg ggacagcttt
    42001 acaggcgttt acgatcaatc cacttatacg atcaggaaag acagcaaaac ggatcatgga
    42061 tgagttgttt attgcacacc agcagtattt accgcaattc aaggagacaa tcagccgatt
    42121 aaaagaagaa ggaatcgaag ttcaagatga actggcaaga gaattatcag gtaatacaca
    42181 tataatggag gaagtataat gggattcaga aaagattttt tatggggcgg tgctacagca
    42241 gccaatcaat gtgagggcgg ttatgatgaa ggtggacgcg gactagcaaa cgtagatgtt
    42301 gtgccaatcg gaaaagaccg ttttcctgtt atcaccggaa agaaaaaaat gtttgaattt
    42361 gatgaggatc atttttatcc agcaaaaatt gcaatcgata tgtatcatcg ttataaagaa
    42421 gatattgctt tgtttgcaga aatggggttc aagacttatc gaatgtccat cgcctggagt
    42481 cgtatttttc caatggggga tgagaaagag ccgaatgaag ctggcttgaa attttatgag
    42541 gatgccttta aagaatgcca aaagcacgga attgagccac ttgtgacgat tacgcatttt
    42601 gattgtccaa tgcatttgat caaagaatat ggtgcgtggc gttccagaga actcgttggt
    42661 ttttatgaaa atctgtgtcg agtcatcttc aatcgctata aaggacttgt caaatattgg
    42721 ctgacattca atgagatcaa tatgatcctt catgcaccat tcatgggcgc agggctgtat
    42781 tttgaaaaag gagaaaatga agaacaaatc aaatatcaag cggcacatca tgagttggtt
    42841 gcaagtgcca tcgcaacgaa gattgctcat gaagtagatc cggagaacaa agtaggctgt
    42901 atgctggcag caggagcata ttatccatac acttgcaatc ctgaagatgt ttgggcttct
    42961 agaaaatcag atcgagaaaa ttatttcttc attgatgtcc agtcacgagg agaatatccc
    43021 gcttatgcat tgaaagaaat ggagcgaaat ggcattcaga tcgaaatgga accaggggat
    43081 gccgcattgc tgaaagaaca tactgttgat tttatctctt tttcttatta tgcttcccga
    43141 gtttcgacta ccgacaacga actcttggaa cagactgcag gaaatatctt tgcttctgtc
    43201 aaaaatccat atctgcaagc aagtgaatgg gggtggcaaa ttgatccatt aggcttgaga
    43261 ataacgatga atgatattta tgatcgttat caaaaaccat tgtttattgt agaaaacggc
    43321 ttaggtgcag tcgatacgcc agatgaaaat ggttatgtgg aagatgatta ccgaatcgat
    43381 tacctggcag cgcatatcca ggcgatgaag gatgcagtag aactagacgg agtcgatcta
    43441 ttaggctata cgacttgggg atgtattgat ttagtatcag ctggtaccgg tgagatgaag
    43501 aaaaggtacg gattcattta tgtcgatcgt gataaccaag gaaatggcac attagcccgt
    43561 tcaaagaaaa aatcattcaa ctggtataaa aaagtaattg attcaaatgg agaaaatctt
    43621 tcgaacgatt gatcaatgat ttataaaagt ctattgtttt tcagcggatt tttcgagtat
    43681 aataaagcta ttcgagaaac gcaagcagaa caatagactt tttttcgcat tggctgtaaa
    43741 gtatgcttac ttgattaagt aaaggagaat ctacatatgt atgatgtaat cgttattggc
    43801 gcaggtcctg ctgggatgac tgctgcttta tatgcttcac gttcaaactt atccgtattg
    43861 atgatcgaac aaggtgcccc tggcggccaa atgaacaata cggcagaaat cgaaaattat
    43921 cctggattcg attcgatcat gggccccgat ctagcttata agatgtatga aagtgtctca
    43981 agatttggga cagaaaatgc ttatggaatc gttcaagata tcaaggatca cggcagccat
    44041 aaagaagtca tctgtgaaga taaaagttac gaaggaaaaa cagtcattat cgcaacagga
    44101 tgtatccacc gtaaattagg cgtacctggc gaggaagaat ttgctggacg aggcgtttct
    44161 tactgtgcag tctgtgatgg tgcgtttttc cgtaacaaac gtctagtagt catcgggggt
    44221 ggggactcag cagtagaaga agccatctat ttgacacaat ttgcttctga agtagtgatc
    44281 gtccaccgta gagatgaatt acgtgcacaa aagatcatcc aagaccgtgc atttgcaaat
    44341 gaaaaaattt cctttatgtg ggatacagtc gttgacgaaa tcgttggaaa cgaaatggta
    44401 gtgacaggtg tgaaagcacg taatgtcaaa accggcaatg aatttgattt agcagcagac
    44461 ggagtattca tctatgttgg actggatcca ttaactgcac cttttagaga agcaggtttg
    44521 acgaacgaag ctggctggat tccaacagac aacgaaatga aaacgaatat gccaggcgtt
    44581 tttgccatcg gagatgttcg tgaaaaagat ttgcgacaaa tcactacagc tgttggtgac
    44641 ggcggtattg ctggacaaca agtgtataaa tacttggagt ctttagaaga aacaacagca
    44701 taagtttaaa aagagtagaa gaacgggttc aattgttctt ttactctttt ttttcgcgtg
    44761 aaatttactg aatctgattt atgcgaacac gtccaacacc ttttgataaa agaatccttt
    44821 cttgatttaa gcgttttttg gtcctcatac tttcatatct aataagtgaa tgctatactt
    44881 attaagaacc atatttcaga aagaggtgtt attttcatgt cttgggaaca agtgtatgaa
    44941 caatgggtac atgaagaaaa tttagaagaa aacttaaaaa aacaattaaa tgatctgggt
    45001 gaagatcctg aaaaacttga agatgctttt tatgcaccgt tggaattcgg tactgcaggt
    45061 atgcggggaa tcttaggacc tgggatcaat cgaatgaaca tctatacggt aagacaagca
    45121 acagaaggac tagcacgttt catggataca caaggtgaag aaacaaaacg ccgaggtgtc
    45181 gctatcgctt atgactcacg ccatcagtca ccagaatttg caatggaagc agccaaaaca
    45241 ctagcacatc acaatatccc gtcttatgta ttcgaaagtc tgcgtccaac accagagctt
    45301 tcttttgcag ttcgtcattt gaagacgttt gcgggaatca tggtaaccgc atctcacaac
    45361 ccagcggctt acaatggata taaagtctac ggagaagatg gcggacaaat gcctcctgca
    45421 gacgcagatg cattgacgaa atatgtccgt gaagtcagca atccgctaaa agtcgaagta
    45481 ttatctgacg aagaagctaa acatagcggg ttgatcacga tcatcggaga agaagtggac
    45541 gctgcttatc tagaagaaat caaagcagtg acgattgatc gtgaattggt tgaaacaatg
    45601 gggaaagatt tgaaacttgt gtacacgcca cttcatggaa cagggaaaat gttgggcgaa
    45661 cgtgcgttga aacaagctgg attcgaacaa ttcgtactag ttcccgaaca agctgtggca
    45721 gatccagatt tcactacggt caaatcgcca aatccagaag aacattcagc ttttgaatat
    45781 gctatacgac ttggagaaaa agaaggagct gacttgctga ttgcaacaga tcctgatgca
    45841 gaccgtttag gcgcagcggt tcgtttgcca gatggttctt atgaagtatt gactgggaac
    45901 caaatcggag cattgtttgc tcaatatatt ttggaagcac atcaatcaag aggcacttta
    45961 ccagaaaacg cagcggtatt gaaatcaatc gtttccagcg aattgcctac agcaattgcg
    46021 gaacactacg gtgctaccat gttcaatgtg ttgacagggt ttaaattcat tgctgaaaaa
    46081 atccaacaat ttgaagaaga ccattcacat acttttatgt ttggattcga agaaagttat
    46141 ggttatttag ttaaaccttt tgttcgcgat aaagacgcga tccaagcatt gcttttatta
    46201 gcagaagtag cagcttacta taaaaaacaa ggcaaaacgt tgtatgacgg cttgcaaacg
    46261 atttttgaaa cctatggcta tttcgcagaa aaaacgattt ctgtgacaat gagcggacaa
    46321 gaaggttctg cgaaaatctc tgctttgatg gaaaaattcc gtaaagaagc accgacagaa
    46381 tttgccggag caaaagttat tcaaacagaa gactttaaag aattgacgag aacagaccgt
    46441 gaaggaaacg tagaaaaact aacaacaccg ccatccgacg tattgaagta tacattggaa
    46501 gaaaatggct ggatcgctat ccgcccttca ggaactgagc cgaaaatcaa gttttatatt
    46561 ggtgtaaaag gaagctctac agaagacgca gccgaaaaag tagcctctta tgaagcgatt
    46621 atcaaaaaga tgacagaaga ataaaaaact tgagactgat ggaaaaagtg gtaaacttta
    46681 gaagtcagtc ttttttcaag ttggaggtga atggattgcc agacacgcaa aaatcaaaag
    46741 tagagaaagt cagtcaatta ttcaaaatgt tgagtgatcc cactcgtctt aaaattctgc
    46801 tatatctgaa agacggggaa caaaatgtga cagcaatcac ccaagcagtg gaaatggagc
    46861 agtctgctgt atcgcatcaa ctacgtttat taagagaaaa tcatgtcgtc aagtctcatc
    46921 gcgagggaaa agcgattttg tatagtcttg atgattctca tgtactggat attttgaacc
    46981 agacactcaa acatgtggaa catcaataac gataggtgaa aatagataaa cactggagtg
    47041 tgacaacgaa gaaatttcgt tgtcacactc cagtgttttt taagaaaaag acaaaaatga
    47101 ttaatcgctt ttttctgttg tttcagttcc ataataacta tgatcttcta ggtctaaagc
    47161 attcaaaata agcgaagccg tttcttcaat agataaagat gccacattga tcactacaca
    47221 tcctagtttt tcataaagat cattcgcaaa atcgagttca gctctaattt tttcgatatc
    47281 ggaataagca gtatcagggt ttaatccgta agagcgcatc cgttctttac ggatattatt
    47341 taaaatatct gggttattcg ttagtcccac gattttttta ggatcgattt cccacagctg
    47401 tttgggaata tgtgcttggg gaatcaaagg taaattagca acttttaaat ttttatttgc
    47461 taaaaacaag ctcaaaggcg ttttggaagt tcgggatacg cctagcaaaa cgacatctgc
    47521 ttctaaaaaa ccacgaggat ctttcccatc atcgtatttt acagcaaatt ccattgcttt
    47581 gatccgtttg aaatagttct cattcaataa atggagggca ccaggttgcc gaattggctt
    47641 gataccagtt aaacgttcaa tttctgatat cggtggcgtc atcacatcga aactaaataa
    47701 ttgatgttct tcacaaaaag aacgtgttat ctcgatcagt tcttgattga ttaatgtatg
    47761 caggacaagt gctttttctt ctaatgcatc ttgcaatgcc tgccttaatg ttttttcatc
    47821 tgtcacgaac gttctgcgga ataggttgaa ctcaacggta ggatactgag ccatagtagc
    47881 ttgcgctagt tttgtcgcag tttctccagc agagtcggag atgacaaaaa aagtaacgat
    47941 ttctgtagaa tcggaagaca tggataaaac ctcacttgca ttttttttat gtttattata
    48001 ccatactcta gaatgcgtac ttcgtaatca ttttgatttg gaggaataag tcatgaaaaa
    48061 acagacattg atcgaacaat cattagaaac attgaaaaat aacggtttta aatatactaa
    48121 aaaaagagaa gcattgttaa cttatttagt gaagcgtaac cgctacgtat ctgctaaaga
    48181 agtttttgac tatatgaatc aggaattcaa aggggtaagt tatgatacga tttaccggaa
    48241 tctccatgat tttgcagaga tcgatttact ggaagaaacc gatttaaatg gtgaaatgaa
    48301 gtttcgtttt cattgctgtc agggggaaat cggccatcac catcatttta tctgtacagt
    48361 gtgcggaaca acgaaagaaa tccatatgtg tccaatgaat tatttcgaag aacagctagg
    48421 tggatgcacg atcgaagggc atagattcga gattttcgga agatgtgaaa attgtcaata
    48481 aggatttaaa taaaataaca tttgataatt taaaaaaaaa gcaagatttc cttgacggtc
    48541 aaaacagcat tagatataat atttacagga cagtcttgtt ttttgagata tatttcaata
    48601 acaattacgg ttacttttta agctcggagg gagggaatta acatgtcaaa aacagtcgtt
    48661 cgcaaaaacg aatcacttga cgatgctctt cgtcgcttca aacgttccgt gtcaaaagcg
    48721 ggtactttac aagaatctcg taaacgtgaa ttctacgaaa aaccaagtgt aagacgcaag
    48781 aaaaaatctg aagcagctag aaaacgcaaa aaattctagt tctgtgatga attggagttg
    48841 gtagtgtgtc actactaagt agattaaacg atgacatgaa agcagcgatg aaagcgaaag
    48901 ataaggaatc tttacaagtc atccgaatga tcaagtcatc tatacaaaac gagcagatta
    48961 aagaaggtca cgacttgaca gaagaagaag aactaacggt gttgtctcgc gaaatgaaac
    49021 agcgacgtga ttctttgcat gagtttgaag aagcaggacg tgacgattta gctgaaaaag
    49081 taaaaagtga aattgtaata gtcgaaaaat atatgccgga acaactttcc gacgaagaaa
    49141 ttcgacaact tgttcaagaa gcaatcactc aaactggtgc ttcatcaatg aaagaatttg
    49201 gcaaagttat gggtgcaatt atgcctaaag tcaaaggaaa agcagatgga aaccaagtga
    49261 atgcgattgt gaaagaacta ttacaatctt aattttatca tttaaactgc ggacagtgat
    49321 tgtccgcagt tttttgtgtt cgtaaaaaaa gaactaaaaa acagctagaa gaaagaataa
    49381 gattctttct tctagctgtt tttttcctgt ttaatatctc ttatgttaaa ttgtgtttga
    49441 tttggttatt cttctggtag ttcatttgct tcttgtttat accctgatag gcgataggcg
    49501 taataaaaga gtaagctgcc agcgtaacca agtaacgttg ggaccatcca gccaagtcct
    49561 aatgcagcaa aaggaacgtg atgatgataa aatcctagta agcttgctac gacagtttgc
    49621 tgactcacta atgcaggcga atttgccaac atatccaata cggctggaac ggcagaaaag
    49681 aacatcgtta cttgatagac aaatggactc ttttggaaga attttgctgt taaggaaagc
    49741 aagattaacg caagtgacaa tggatacaaa agcatcaata ctggtaaaga ccattggatg
    49801 atattatcca atccggcatt cgcaacgaca aaagagacaa aagtcgttaa ccgcagccat
    49861 gtcatataac tgactttagg gaacagttta tggaaatctt gtgcaaaaga aacaacgagt
    49921 cccatagcgg tagtgaagac acctaatgtg accattaccc ctaagaagct agcaccgaag
    49981 ttacctaaat aaaaattagt gatctgagaa agtgcagttc cgccattttc tgataaaggc
    50041 atcttattca aactcatcgc accgagaagg accagaccaa aataaatcat gacttctaac
    50101 gcaacgctca ggctaccagc ttttgctgtt aattctgtga cttttttatc tttaaaacca
    50161 agatcacgaa ctgcatggac aaaagtgaca cttaaagcaa gtaaagctac tgcatcaacc
    50221 gtgttgtatc cttctaaaac gccatttgtc aaagcgttcg tctgataaga atctgcagca
    50281 gattggttta gattccccat gggttggaat aaagaaacga caaaaatgat agctaataat
    50341 gcaagaaaca aggagtttaa atactttccg acatatttga ccaaacgtgt tggatgtaca
    50401 gtaaggaaat aagcaagtaa aaagaaaaga gcagagaata aaaacatacc taatgaatca
    50461 tatttttcag gcaaaaaggg tttagcagcc atttcaaaag cagttgctgc tgtccgcggt
    50521 gtaccaaaga atggtccgat cgttaaatga acaaggatca agaacaaggc agcataccat
    50581 tttccgacag gtttagctaa gtcatacaga ccatcacttt ttgtcactac tacagctaag
    50641 atagctaata atgggaaaag agcgcctgag atggcaaatc ctacaccagc gctgatccag
    50701 tttgatccag ccatttgacc taagtggaca ggaaagatca gattacctga cccgaagaac
    50761 atcccgaaga taagtgaact gaccatcaat aattgcttcc atgaataagt ttttgtttct
    50821 tccataaatt tttcctccaa taattatcat tttgagtaac aaaataaacg ttgcagaaac
    50881 tagaaatgcc actaatatca gattccatga aaaccaccac cttaaaatat aaaaaaaagc
    50941 ccttttccac ttgggaaaag ggctctagat tctagaacgg taccaccttt tttcgcttaa
    51001 aaaagcttct tcattcacgg acgcttttcg ataaagttat ccgctagcct gataatggag
    51061 gcagccacaa tgatctacgt tttcgacatt gactcggaaa tgatgttcgt ctattcgatt
    51121 tattgtcatt tctcacttac atgactcact gggaataaat cgctgaatag cctactgttt
    51181 tcctcgacgc tttgctgtat atttatttgt tgacgttata atacacctgt tttttttgaa
    51241 atgcaagaat atttcaaata ttttgttttc tttttttgaa acaagaaaaa tacccagaga
    51301 atacttgcta agttagacgg aatacttgtc ctctatctta tgcatagctt acatgatcag
    51361 ctttttttat tcaaacgaga cctttagaga atggaagcag ctatttttta acgtttcttg
    51421 gtacttatgg ttaagcaact cttttagaac cttttcttat ttgctatata gtttcttctg
    51481 agtatggtaa aataggaaag aatgaatcga aaaaaagggg atgtctattt ttgacggaag
    51541 agtctagttc tttagagatt agattgacgg ctgctgatga tgtcagcatg cttttaggtg
    51601 cacacgacaa gcatataaaa ctgattgaag aaacaacaga aacaaccatc catacacgtg
    51661 gagaaatcat tcagatcatt ggcgaacaat cccagatttc cttggctgcc tcagtcattc
    51721 ggacattgca ggaattgatc aaacgaggaa tccatgtaag tacaccagat gtcgtgacgg
    51781 cactgaaaat ggggcaaaaa ggcacattag attattttgt tgaaatgtat gaggaagaga
    51841 tcgtcaaaga ccgtaatgga aaaccaatac gggtaaaaaa cagcggccaa aagaaatatg
    51901 tcgaatcggt agccaaacat gatatcgtat ttggcgtagg accagccggg acaggaaaaa
    51961 cttttcttgc agtagtatta gcaatcgctg ccttgaaaaa aggtgaagtg caaaaaatca
    52021 ttttgacgcg gcctgctgtg gaagcagggg aaaatctagg ctttcttccc ggggatctaa
    52081 aagaaaaagt agatccttat ctgcgtcctg tttatgatgc tttataccaa atttttggat
    52141 tagatcatac gaaccgattg atggaacgag gcgtgatcga aattgctcct ttagcttaca
    52201 tgcgggggcg gacattggat gatgcattcg ttattttgga tgaagcacag aacacgacag
    52261 ttgctcagat gaaaatgttc ctgacacgtc ttggttatca atcaaaaatg attgtcaatg
    52321 gagatacgag tcagattgat ctgcctcgag ggacaacaag tggtcttgtt catgcagaac
    52381 gaacattaaa gcaaatcaag aaaatcgatt ttgttaattt tgaagcaagt gacgttgttc
    52441 ggcaccctgt agttgccgaa atcatcaaag cttatgaaaa ggcagatctc catcaagaat
    52501 aagctttgct taggaggata agcacatgtt gaataaaaca ctgcagaaac tacaagaaaa
    52561 acttggaaag gcagcattgc ctttcctgct gttcttgttt tttatcatta tctgcggcgt
    52621 tacatttagt agtgtcaaac aaaaggccaa tgatttcaga gaaggccaag tagcagaaga
    52681 aagtatccgt gcgaacaaaa cgatcgagaa cacggaagaa accgaacaaa aaagaaaact
    52741 ggcagcagaa gcagtcactc cagaatacac ttaccaaaaa gatcttgcag atgaccagaa
    52801 caatcggatc aagcaactat ttgaattgat cgataaaacg aatgatgcca tcaacaaatc
    52861 ttatgacgaa aagattgata aggcaaagga agatgaaaat atcccaaagc ctactacaga
    52921 agagcggatc gccgcgttga agaaaaattt tgaaaatgtg aatgcagaaa atgttgcgtt
    52981 ttatcaaaaa ctaccgaaca atttttattc gacgatattt gaaatgactg aaactgagat
    53041 ccatactgtg agggatgaga gtctgaagct cattgacgaa cagatgagca aacaagttcg
    53101 tgaaagtgaa ttggaagctt ttaaacaaga agcagaagag caaatccaat atttgaatgt
    53161 cacaccagca caacaacaaa tgattcgcta cttagtggat caaggtatcg tagtgaatga
    53221 cgtattgaat gagaaaaaga cagaagaatt gaaacaatcc gcacgagaag ctgtacaacc
    53281 agtgatgatc ttccaaggag aaatcattgt tcgtgaaggt aatcaaatcg atgcttcagc
    53341 tatgaaaaaa ttggaattac taggactgac taatcaaaca acttcgattt ttccgctaat
    53401 tgccatggtt ttagctgttt tgcttcaaat cggcgtactg atttataatt cacttcaatt
    53461 ccaaaatgca ggagaaagaa caaaatacgt gcttttctat gtgacagcta tgtcagtcag
    53521 catagtgctg atgaaatttt tccaattatt ccaaacggaa caagctactt atatcccgct
    53581 gttttatccc gctgcttttg caccacttgt tttaagcttt ttcttgaacc gaagagcagg
    53641 gatcatggct ggtattttcc aagcagtatc tgcacttttc attttttatg attcgattgg
    53701 cacgaacttt ctaacgatta tcttgatgtc ttatgttttc tctgggctca tggcaacgat
    53761 tgtcagacgc aaacggatct cagaacaagg attatctgct gccatgtggg tcattatttt
    53821 tcctgtgatg atggatgtca ttttgatcgt ctatcaagga atgagctttg gtgatgcaac
    53881 gacttggtta atgcttgtct gcgggttcac tggtgcactc ttctcattct tgctgacgat
    53941 cggtctacac ccatatattg aattgatggt caatgatgac agtgtaattg tattaaatga
    54001 actcagcaat ccaaatcatc ctttattgaa acagttgctg gaagaagcgc caggtactta
    54061 tcatcatagt atgatggtag ctaatctaag tgctaacgct gtagcagaaa tcggcggacg
    54121 ttctttgcta actcgagtag cttgctatta tcatgatatc ggcaagatca aacatgctag
    54181 ctttttcgtt gagaacttgc cttcaggagc ggaaaacccg cacaatttct tattgccaga
    54241 agacagtaaa catattatct ttggacatgt gacagatgga gccaaaatat tggaagatta
    54301 taatatgccg caaatggtga tcgatatttg caaacagcat catggaacca cgctaatgcg
    54361 ttatttctac atcaaagcga aagaacgcaa tccggaagta acagaagagc aattccgtta
    54421 tcctggacca aaaccgcaaa gtcgtgaagc agggatcgtc aatattgctg acagttgtga
    54481 ggctgcagta cgggcaatgg atcatccgac gagcgaaaaa atcgaaagtt ttgtccataa
    54541 tcttatcgaa gagcgtattt ccgatggtca attagacgat agtggactga ccttgaaaga
    54601 aatccgaaaa gtggaaaaat cattgatcag cggtttaagt agcaccttcc attctcggat
    54661 caaatatcct aaaatgaaaa cagaagttga gaagataaag gaagaacaag aagaatagaa
    54721 agaagcgatg tataatggat attaccttta tggatgaaac aaaccaagtc tcagaagaga
    54781 aaatgaaaga aatcgatgac ttgctgcaat ttgcagcatc ttatttaaag ctgccagagg
    54841 aaacagaaat gtctgttact ttcatggaca atgcggcaat ccaagtaatc aatcgtgatt
    54901 accgtggaaa agatgcaccg acagacgtca tcagttttgc tctggaagaa gaaggcgaag
    54961 acgaaatccc agtgattttt gaagatggag agaatccatt gcctcgtgta ctaggagaca
    55021 tcatgatttc tattgaacgt gctgctgaac aagcagaagc ttacggccat tcatatgatc
    55081 gggaacttgg ctttttagcg gtacatggat tcttgcatat caacgggtat gaccatatga
    55141 caccagaaga tgagaaagaa atgtttggtt tacagaaaga gatcttggat gcctatggac
    55201 tcaaaagata aaaacgtaga gaaaaacaaa catttcctga tgtctgtgga atttgctgtc
    55261 caaggagtca aaaccgtttt tgaggaagaa cgcaatatga ggaaacatgt tgcgttcggt
    55321 atccttgctt tatttatggg cgtgattttc cagcttgatc ggatagagtg gttatggctg
    55381 cttttagctg tttttctcgt ttggatagta gagatcatca atactgtttt tgaaaatgta
    55441 gtggacatgt ttacggattt tcattttcat ccgattggga aaaaaatcaa agatatggca
    55501 gctggagcag ttttagtgac cgcttgtttt gctgtcatca ttgggttgat tttatttgtc
    55561 cctaaaatat atcatttgtt gtttaattaa aaaggagaac gtatatgcca gaacataaat
    55621 cagggtttgt cgcaatcgtc ggccgcccga atgtaggaaa atccacatta ttgaaccgaa
    55681 tcgtgggtca aaaaattgcc attatgagtg acaaggcaca gacaactaga aataaaatcc
    55741 aaggagtata tactacacca gaagctcagc tgatttttat cgatacacca ggaatccata
    55801 aaccaaagca tcgtctagga gattttatgg tagaagcagc ttacagtgcg ttgaaagaag
    55861 tggatgcagt cttatttatg atcagtgctg atcaaaaacg cggccgggga gacgatttta
    55921 ttattgaacg tctaaaaaac gtgcattctc cagtttatct gattatcaat aaaatcgata
    55981 agatccatcc agacgagttg ctgggaatca tcgaagatta ttcgacgcag atgccttttg
    56041 cacaagtgat tccgatctct gctacagaag gaaacaatgt cgaacgctta atggatgtat
    56101 tagtcagtga aatgccagag ggcccacaat attttccaga tgatcaagtg actgatcatc
    56161 ctgaatactt tatcgtttct gaattgatcc gtgagaaagt cttgttttta acacgagacg
    56221 aagtgcctca ttctgtcgca gtcgttgttg attcgatgaa gcgaaacgag aatgataaaa
    56281 tccatatcca ggccacgatc atcgtcgaaa gagatagcca aaaaggaatc attatcggca
    56341 aaggcggaaa aatgctgaag caagtaggaa cgaaagcaag aaaggatatc gaaaatcttt
    56401 taggagacaa agtatttcta gaactttggg tcaaagtcca aaaagactgg cgagacaaaa
    56461 aagtctatct gcaagacttt ggctaccgaa aagacgaata ctgatattct gaacttctta
    56521 taggttaagt atagaaataa actggataaa aatccgcacg ttgagaaaaa cggcggaaga
    56581 tcgaaacata aataatctgg atcggcagaa tctgcttttc cagatttatt ttttaggagg
    56641 gagaatatga gccagcttgc agaatcgaaa ggcttgattt tatctagccg taattacaaa
    56701 gaaaaagata aattagtcaa aatctttacg gaatcttctg gcaaaatgat gttttatatc
    56761 aaaggaatcc atcggcaaaa tcagccatta gctccggctt tgctcccctt taccgaagca
    56821 gtctatatcg gtaattttcg aagtgaaggt ttgtcgtttt taagcagtgt caaagacgtc
    56881 catgcgtttc ggaacatcca agaagatatc tttttgagcg gttatggtac ctatttgcta
    56941 aatctagtag atgcagcaat cgaagatcat caatatgacc cgcatttatt ccaatttacg
    57001 caacaagctc tgcagcggat ggatcaagga gatgatgcag aaatcatcac gaatatcttt
    57061 gaagttcaaa tactgcaacg cttcggaatc gcgccaattt ggacgcactg tgtcgtatgc
    57121 ggggaaacaa aaggaaaatt tgactattct tccaaatctg gcggtgtgat atgcgagaaa
    57181 cactggccaa tggacgaaca ccgttatcat gcagatcctc gcgctgttta tttcgttcga
    57241 atgttttcgg ctatttctta tgataagatc agtggaatcc acttgaaaga agaaacgaag
    57301 caggcaatcc gtgacctcat tgaccagttg tatgatgaat atgtcggtat tcatctaaaa
    57361 agcaaaaagt tcattgatca aatgaagtct tgggcacacg tattaaagac gccagagaaa
    57421 aatatgcctg aaaaagagat acctgaaaaa gaagaataaa tgaaagatac aacaaaaaaa
    57481 tcaaaactat ttgacaaata cagggacttc cgtctattat taaccgtaga ttaaatagat
    57541 cgttcactga gaggatctat tatataaaca agaaaactgc aaaatgaata cgtacattga
    57601 ccaagatcag taaaaaacac gtggtttagc gagttcggga gagtggaagc cggacccaaa
    57661 cgatttttga agaggcactt gcgagtacga taaatgaagc tgccgaatct ttattcggaa
    57721 gtagggtgga accgcgaaat tattcgtccc tatgtctgta atcagacata gggacttttt
    57781 gtcgttttct ttcctgtatt cttattgaca gcaacgctta aaaattgaaa ggggcaaaac
    57841 agatgagtaa aaaattgacc gtccaagata tgattttaac attacaaaaa ttttggtcat
    57901 caaacggctg catgctgatg caagcttatg acacagaaaa aggagcaggt acgatgagcc
    57961 catatacatt cttacgggca atcggaccgg aaccatggaa tgctgcttat gtagagccat
    58021 cccgccgacc agctgacggg cgttatggag aaaacccaaa ccgtttgtat cagcatcatc
    58081 aatttcaagt cgtaatgaaa ccttcacctg aaaatatcca agagctttat ttagaaagct
    58141 tgcgtctttt aggaattgat ccattagaac acgatattcg ttttgttgaa gacaactggg
    58201 agaacccatc gatggggtgt gccggtgtgg gttgggaagt atggcttgat gggatggaga
    58261 tcacacaatt cacgtacttc cagcaagtcg gaggactacc ttgtaaacca gttacttctg
    58321 agatcactta tggactagaa cgtcttgctt catatattca agaagttgaa agtgtctatg
    58381 atctggaatg ggctgatggt gtgaaatatg gcgagatctt caaacaacca gaatatgaac
    58441 attccaaata ttcttttgaa gtaagtgacc aagacttgct actaagcaac tttgaccgtt
    58501 ttgaaaaaga agctaaacgc tgtatcgatg aaaatcttgt tcatccagcg tatgattata
    58561 ttttgaaatg cagccataca ttcaatttat tagatgctcg cggtgcagta tccgtaacag
    58621 aacgagcagg ttatctttcc cgtatccgca acatggctcg tgctgtagcc aaaatctttg
    58681 tggcagaacg cgaaaaatta ggattccctt tattgaataa aaacacagca aatccagaaa
    58741 acatgaagga ggctgaataa gatggcaaaa gacttattac ttgaaatcgg gctggaagaa
    58801 atgcctgctc atgttgtcac accaagcatg aaacaattag aagaaaaaac agcaaaattt
    58861 ttagatgaaa accaattgac ttatgatacg atcgaaacgt tttctacacc gcgccgctta
    58921 gcaatcaaag taacaaacat tccagaacgt caagcagaca gtgaagaaga agtcaaagga
    58981 ccagccaaaa aaattgcttt ggacgcagat ggaaactgga cgaaagcagc tgaaggattt
    59041 gtccgcggac aaggtctgac gacagatgct atcactttta gagaattaaa aggcgtggaa
    59101 tatgtctatg taacgaaata cactcacggc aaagaagcga aagaagtatt gacaggttta
    59161 gcagatgtca ttaaaagcct cactttccca gtaacgatgc attgggcaga atacgatttt
    59221 gaatatatcc gccctattca ctggatcgtg gcattgttag atgacgaagt aatcccgttt
    59281 gagatcttag atgtcaaaac aggcagagcc tctcgaggac atcgtttctt aggcgaagat
    59341 gtgacatttg cgcatgcaaa agaatatgaa gaaaaattga atgaacaatt cgttattgct
    59401 tcaccgcaaa aaagacaaga aatgatcgtt acacaagcaa atgaattagc cgaaaaaaat
    59461 cactgggtta ttgatctaga cgaagattta ctagaagaag tcaacaattt agtcgaatat
    59521 ccaactgtct tttctggcga cttcgaagaa aaatatcttt ctgttcctga agaagtacta
    59581 gtcacttcta tgaaagaaca tcaacgttat ttcgaggtac ggaacgatca aggtatgctg
    59641 cttcctcatt ttatttctgc tcgtaatggt gatcgggtaa aattggaaaa tgtggttcga
    59701 ggcaatcaaa aagtattgac ggctcgttta gaagatgcgg aattctttta taatgaagac
    59761 aagaaagctt caatcgacca atacgttgaa aaactaaaaa ctgttacttt ccatgaaaaa
    59821 atcggtagta tctatgagaa aatgcagcgg gtacaagtca ttgcccgtat cattggcgaa
    59881 caagtaggac tatctgagga agaattgatg gatctgcaac gagctgctga aatctataaa
    59941 tttgatctag taaccaacat ggtaggggaa tttcctgaac tacaagggat catgggcgaa
    60001 aaatatgctc tgctgaaagg cgagaaacct gctgtagcaa cagcgattcg tgagcattac
    60061 atgcctatat ccagtgaagg agagctacct cagtctacag taggttctgt tttagcgatt
    60121 gcagataaat tagatagcat tttttctttc tttgcagttg ggatgattcc aagcgggtca
    60181 aacgatcctt atgccttacg tcgccaagca tacggtgtcg ttcggatcat cgaatcgaaa
    60241 aaatgggcct ttcctttgtc tgttctgcaa gaaacgatta gtgatgttat cagtaaagat
    60301 acggatcgtt tcggcattgg tttatcagaa gggcaacaac aggtgatcga ctttatcaaa
    60361 ggccgtctgc gccaattatt gacaacgaag aatattcgtc atgatgtcat cgaagctgtt
    60421 ttgaatgcag aacaaaaaga tctgacaaaa gtatttgcag cagcacaatt gttcaagcag
    60481 catcttgcag atgaagactt caaaccatcg atggaagctt tgacacgtgt cgtgaactta
    60541 gcgaaaaaag cggagcttga aaaacaatca gaagtagacc ctgaactgtt cgaaaatgaa
    60601 gcggagaaag aactgcacaa agcagtagag gcaatgaaaa aaagctttgc tgaccaaacg
    60661 atcaacgaaa attatgatca gcttgtaagc cttcgcccac tgatcgaaaa ttattttgat
    60721 cagacgatgg tcatggcaga agaaccagca gtacgtaaga accgattaac gcaattaagt
    60781 caaattgctg ccatggcttt gtcgctagca agtttagatc aaatcatcac aaaataattt
    60841 ctaatatgta aataaaccag agaataggca caaaa
//
LOCUS       DAYYFU010000011        60192 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000011 DAYYFU010000000
VERSION     DAYYFU010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.011"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="ACHA34_000820"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="ACHA34_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFM8791486.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="ACHA34_000821"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="ACHA34_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFM8791487.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="ACHA34_000822"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="ACHA34_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFM8791488.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1366..1491)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1642..2274
                     /locus_tag="ACHA34_000823"
     CDS             1642..2274
                     /locus_tag="ACHA34_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8791489.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="ACHA34_000824"
     CDS             complement(2276..3181)
                     /locus_tag="ACHA34_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8791490.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3925)
                     /locus_tag="ACHA34_000825"
     CDS             complement(3266..3925)
                     /locus_tag="ACHA34_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8791491.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="ACHA34_000826"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="ACHA34_000826"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFM8791492.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="ACHA34_000827"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="ACHA34_000827"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFM8791493.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="ACHA34_000828"
     CDS             6168..7253
                     /locus_tag="ACHA34_000828"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFM8791494.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /gene="fni"
                     /locus_tag="ACHA34_000829"
     CDS             7250..8314
                     /gene="fni"
                     /locus_tag="ACHA34_000829"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFM8791495.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="ACHA34_000830"
     CDS             complement(8563..9366)
                     /locus_tag="ACHA34_000830"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFM8791496.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="ACHA34_000831"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="ACHA34_000831"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFM8791497.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="ACHA34_000832"
     CDS             complement(10244..11251)
                     /locus_tag="ACHA34_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM8791498.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="ACHA34_000833"
     CDS             complement(11258..11902)
                     /locus_tag="ACHA34_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM8791499.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="ACHA34_000834"
     CDS             complement(12002..12760)
                     /locus_tag="ACHA34_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFM8791500.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="ACHA34_000835"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="ACHA34_000835"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFM8791501.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="ACHA34_000836"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="ACHA34_000836"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFM8791502.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="ACHA34_000837"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="ACHA34_000837"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFM8791503.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="ACHA34_000838"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="ACHA34_000838"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFM8791504.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="ACHA34_000839"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="ACHA34_000839"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFM8791505.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="ACHA34_000840"
     CDS             18158..19027
                     /locus_tag="ACHA34_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8791506.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="ACHA34_000841"
     CDS             complement(19153..20052)
                     /locus_tag="ACHA34_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8791507.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="ACHA34_000842"
     CDS             complement(20217..21041)
                     /locus_tag="ACHA34_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8791508.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="ACHA34_000843"
     CDS             complement(21052..22347)
                     /locus_tag="ACHA34_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFM8791509.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="ACHA34_000844"
     CDS             complement(22363..22842)
                     /locus_tag="ACHA34_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFM8791510.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="ACHA34_000845"
     CDS             complement(22848..23876)
                     /locus_tag="ACHA34_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFM8791511.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="ACHA34_000846"
     CDS             complement(23893..24999)
                     /locus_tag="ACHA34_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8791512.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="ACHA34_000847"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="ACHA34_000847"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFM8791513.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="ACHA34_000848"
     CDS             complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="ACHA34_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFM8791514.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="ACHA34_000849"
     CDS             complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="ACHA34_000849"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFM8791515.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29970..30668
                     /locus_tag="ACHA34_000850"
     CDS             29970..30668
                     /locus_tag="ACHA34_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8791516.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30836..33475)
                     /locus_tag="ACHA34_000851"
     CDS             complement(30836..33475)
                     /locus_tag="ACHA34_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8791517.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(34094..35125)
                     /gene="queA"
                     /locus_tag="ACHA34_000852"
     CDS             complement(34094..35125)
                     /gene="queA"
                     /locus_tag="ACHA34_000852"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFM8791518.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            35318..36493
                     /locus_tag="ACHA34_000853"
     CDS             35318..36493
                     /locus_tag="ACHA34_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8791519.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            36497..37138
                     /locus_tag="ACHA34_000854"
     CDS             36497..37138
                     /locus_tag="ACHA34_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8791520.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            37135..38052
                     /locus_tag="ACHA34_000855"
     CDS             37135..38052
                     /locus_tag="ACHA34_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8791521.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            38049..38720
                     /locus_tag="ACHA34_000856"
     CDS             38049..38720
                     /locus_tag="ACHA34_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8791522.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(39261..39749)
                     /locus_tag="ACHA34_000857"
     CDS             complement(39261..39749)
                     /locus_tag="ACHA34_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFM8791523.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(39950..40216)
                     /locus_tag="ACHA34_000858"
     CDS             complement(39950..40216)
                     /locus_tag="ACHA34_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8791524.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(40216..40479)
                     /locus_tag="ACHA34_000859"
     CDS             complement(40216..40479)
                     /locus_tag="ACHA34_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8791525.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            41000..41140
                     /locus_tag="ACHA34_000860"
                     /pseudo
     CDS             41000..41140
                     /locus_tag="ACHA34_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(41282..43105)
                     /locus_tag="ACHA34_000861"
     CDS             complement(41282..43105)
                     /locus_tag="ACHA34_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8791526.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(43703..46408)
                     /locus_tag="ACHA34_000862"
     CDS             complement(43703..46408)
                     /locus_tag="ACHA34_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFM8791527.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(46714..47661)
                     /locus_tag="ACHA34_000863"
     CDS             complement(46714..47661)
                     /locus_tag="ACHA34_000863"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFM8791528.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            47851..48120
                     /gene="rpsN"
                     /locus_tag="ACHA34_000864"
     CDS             47851..48120
                     /gene="rpsN"
                     /locus_tag="ACHA34_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8791529.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(48296..48658)
                     /locus_tag="ACHA34_000865"
     CDS             complement(48296..48658)
                     /locus_tag="ACHA34_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8791530.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(48669..49790)
                     /gene="alr"
                     /locus_tag="ACHA34_000866"
     CDS             complement(48669..49790)
                     /gene="alr"
                     /locus_tag="ACHA34_000866"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFM8791531.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(49806..50156)
                     /gene="acpS"
                     /locus_tag="ACHA34_000867"
     CDS             complement(49806..50156)
                     /gene="acpS"
                     /locus_tag="ACHA34_000867"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFM8791532.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(50286..51797)
                     /locus_tag="ACHA34_000868"
     CDS             complement(50286..51797)
                     /locus_tag="ACHA34_000868"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8791533.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(52119..53471)
                     /gene="murF"
                     /locus_tag="ACHA34_000869"
     CDS             complement(52119..53471)
                     /gene="murF"
                     /locus_tag="ACHA34_000869"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFM8791534.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(53624..54700)
                     /locus_tag="ACHA34_000870"
     CDS             complement(53624..54700)
                     /locus_tag="ACHA34_000870"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFM8791535.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            54896..56641
                     /locus_tag="ACHA34_000871"
     CDS             54896..56641
                     /locus_tag="ACHA34_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFM8791536.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(56921..57358)
                     /locus_tag="ACHA34_000872"
     CDS             complement(56921..57358)
                     /locus_tag="ACHA34_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFM8791537.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(57342..58715)
                     /locus_tag="ACHA34_000873"
     CDS             complement(57342..58715)
                     /locus_tag="ACHA34_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791538.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(59037..59525)
                     /locus_tag="ACHA34_000874"
     CDS             complement(59037..59525)
                     /locus_tag="ACHA34_000874"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8791539.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(59547..60149)
                     /locus_tag="ACHA34_000875"
     CDS             complement(59547..60149)
                     /locus_tag="ACHA34_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8791540.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        17104 a        13016 c        10989 g        19083 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac
    27961 aggtgtttgt atagaaaaat atataaagtt atttattatt atttatatga cacacccgat
    28021 tattcaatat tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg
    28081 aggtaggaca tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt
    28141 ccttctcttc tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag
    28201 ctggttcttc tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt
    28261 tgaccgtttt gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa
    28321 ctgtccgttt gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga
    28381 ctgaatcacc tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt
    28441 gcgaacgtga cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta
    28501 atgttcccat ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa
    28561 atgctgataa cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg
    28621 cgttttcttt attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat
    28681 catccatgat tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt
    28741 aagaagtcaa acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac
    28801 gtgtatagtt tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg
    28861 catatttcgc atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag
    28921 cgattcgagt aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca
    28981 atgaatcagc agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg
    29041 tagtgaattc taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga
    29101 ttgagtaacc agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt
    29161 caaaccctgc tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt
    29221 tcaatcctcg ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac
    29281 tttcatcgaa tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt
    29341 ggatgttgat tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga
    29401 aatgcacgcc ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc
    29461 ctgaatctgt taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt
    29521 ctactaagtc ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag
    29581 cacccatttc tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg
    29641 gcatgaacat tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc
    29701 cagtatgttt ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc
    29761 ctactttcta tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt
    29821 tgcaagttcc ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta
    29881 tgaataacaa aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg
    29941 gtgttattaa agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag
    30001 caaaagagag tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat
    30061 tgattggtat tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca
    30121 tatatgatgg atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag
    30181 gcggtggcgg aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga
    30241 cttatcttga tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc
    30301 gtggattcaa aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct
    30361 ttacaacatt atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt
    30421 ctcaaagcta ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac
    30481 tggatacgat cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt
    30541 tagatctttc atttatcggc tggcatatct tagcattagc aactgctggt attggttatc
    30601 tatggctcag cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag
    30661 atatttaaaa gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga
    30721 gaggcagtaa aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc
    30781 acacctcctt tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt
    30841 cctcttcttc tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta
    30901 cgatagataa aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata
    30961 gttcttgcgt aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca
    31021 tttctaccaa agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta
    31081 cattgaacac atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga
    31141 catgctgact tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac
    31201 caaacaaggt tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc
    31261 gagcaaagat cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg
    31321 gctctcttcc taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt
    31381 gcacagctgt aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct
    31441 ccgaaatatt acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac
    31501 gtccttgact gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg
    31561 cggcactttg cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt
    31621 tcaacgctgg tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt
    31681 gccaagattt gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag
    31741 ctttgacacg cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg
    31801 cttctgacgg atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat
    31861 gatctccagt gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt
    31921 tactttctgg acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt
    31981 ccagtgcttc agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa
    32041 tcactctcag tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc
    32101 ctgaactaaa gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt
    32161 cgcccatttg gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta
    32221 ctcgtggata tttttcttct aattctgcct ttgtatgata attctcttca acagccctta
    32281 cgatggctac ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg
    32341 tcacatctgt acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat
    32401 cttcgatatt cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt
    32461 gtgtcagcgt tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg
    32521 ctggcaactg tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg
    32581 tcaacgtaac aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag
    32641 tcatgaacat ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg
    32701 cagccgctaa agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag
    32761 gtgttttttg catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca
    32821 tgccaatggc agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata
    32881 ccatattaag ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt
    32941 caactgcttc actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga
    33001 tcaaacgggc atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta
    33061 ccagttgctc tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt
    33121 tattcatatc ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca
    33181 ataccgagtt gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt
    33241 ctgtagcaaa tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa
    33301 aatcggacaa gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt
    33361 tattcaatcc ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg
    33421 gatctgcttc tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat
    33481 tcctccttta aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt
    33541 atttacttca aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc
    33601 ttatatttgc aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca
    33661 tcaaatagac agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta
    33721 caggtttcac ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat
    33781 gtttggatca cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt
    33841 aaacgtctga aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa
    33901 caaaattttt gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt
    33961 tacaatcatt tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga
    34021 gctcggcttt ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg
    34081 gcttttcata accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct
    34141 cttcaatcgc atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta
    34201 acatgactaa agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga
    34261 attcatagcc aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat
    34321 taaatttcgt tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt
    34381 tcccgccatt tttacgtact tggttcaatc gctcagccgc ttcttccgtc aaacggtaga
    34441 attcactatg catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta
    34501 accctacatg tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta
    34561 attctttcgt aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat
    34621 agacagtttg atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca
    34681 tttctcccaa tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc
    34741 gtccgccgtg ttccaattct tctttcacta ctgcttgcaa tctgccatca ccaaaagaga
    34801 ttcttgtccc aactttggca cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt
    34861 gtgtattcgt tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata
    34921 aacgagcagg cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt
    34981 caatgatttc atggaaatgt ttatcttcca tctctccagt tttccgatcc aaaacaagta
    35041 aacgagaatg atcgcggtct tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa
    35101 aatcaaaatc ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt
    35161 ctatcatgtt tttcgttctg ttggcaaaat cagcactttt tcacttctat tttttgtttt
    35221 cctaaataaa aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag
    35281 atagttcttc attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc
    35341 aaaaatttat aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg
    35401 tgagttcatt tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat
    35461 caaccgaatg actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga
    35521 aattgatcca gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat
    35581 gcctcacatg acgatccgtg aaaatatcac tcttgtccaa aaattgttga aagtcagtca
    35641 agaagaacgg aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat
    35701 gttagatcgc tacccacatg aattatctgg tggtcagcag cagcgtattg gagtcgttcg
    35761 tgcccttgca gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc
    35821 aatcactcgt gattccttgc aagaccttgt caaagattta caagagcgtt tgggaaaaac
    35881 gatcgtattt gtaacccatg acatggatga agcgattaaa ctagcaagcc gtatcgccat
    35941 catgagtgaa ggccgtgtga tccaatttga tacaccagaa aatattttac gtcatcctgc
    36001 aaacgatttc gtagaagagt tgattggaga agatcgccta ctacaagcca agccaaatgc
    36061 gacaagagtc ggcgaagtta tgctaaatac agcaatcacg attacaccgg aaaaatcatt
    36121 acaagaagca atcaaactga tgagggaaaa acgtgtagat acgttgttag ttaccgataa
    36181 ttcgaatgta ttaaaaggat ttatcgatgt agaaacgttg aataaaaaac gaggaaaagt
    36241 atccagtgtc ggcgacattt tgaataaaca tgttttttac gtaaaagaat ccgcctatct
    36301 tcgtgatacc ttgcaacgca ttttaaaacg cgggctgaaa tacgtgccgg tcgttgatga
    36361 acagaacaaa gtcgtaggta tcctgactcg tgcatcatta gtggatatcg tctacgatgt
    36421 gatttgggga gaagatcccg caactactga tgttgcagag tcagctactc ctgaagaatc
    36481 taaggaggtc tgacctatgc agcagttttt tcatcagcac ggttcagaaa ttctctcaaa
    36541 aggttttgaa catctttata tttcaggact tgctttgatc attggtatca tcattgctgt
    36601 accgcttggt gtcttactga cacgttttcc aaaagctgcc acgattgtga ttggcttaac
    36661 cagtgccttg cagacaatcc cttctcttgc tttattggca ttgatgatcc cgctatttgg
    36721 cgtaggtaaa ttacctgcga ttatcgcact ctttatttat tcattattgc cgattttacg
    36781 taatacgtac attgggatga aaaatgtcga ttggaattat cgggatgtgg caaaaggcat
    36841 ggggatgact gagttccaat ccattttttc cgttgaattg cccattgcta tgccaacgat
    36901 catggctggt attcgtttag ctgccgttta cgtgattgca tgggctacac ttgcctctta
    36961 tatcggcgct ggtggtttag gcgatttgat ttttagtgga ctgaataatt accaaccaga
    37021 tctcattttg gcaggtacga tccctgtaac aattttagca ctattggctg attggcttct
    37081 aggtctcttg gaacaccggc tgacaccgat cgcacttaga gaggagaatg aagaatgaaa
    37141 cgtatactaa aaatgaccat ggttcttttc tctcttctta tacttgcgag ttgttccttc
    37201 cctggtcttg ccagcaatac agatgaagac accatttcga tcacaggtgg aatcacttca
    37261 gaagcacaga tcttagcaag tctagttgcc ggaatggttg aacattatac agataaaaat
    37321 accgcaatca tcaacaatct ggctactaca actatcaacc atcaagcgat gatgaacgga
    37381 gatgcttcaa tttcagcggc tcgctacaca ggaacagatt taacgactac attgaacctt
    37441 cctcctgaaa aagatccgaa aaaagcgttt gctaccgtga aagatgaatt tgagaaaaga
    37501 tacggtcaaa cgtggttccc gtcctatagt tttgaaaata cgtatgtctt tcttgttcga
    37561 aaagacaccg cacaaaaata ccatctatct aaagtcagcg atctgaaaaa tgtagcggat
    37621 gaattagtag ctggtgtaga tacttcttgg atcaatcgta aaggagacgg ttatgacggc
    37681 ttccaagaaa cttatggctt ctccttcaat tcgatattac ctatgcagat tggacttgtt
    37741 tatgacgcag tcgaagcagg aaaaatggat attgttcttg gctactctac ggatggacgg
    37801 atcgccagtt atgatctagt catgttggaa gatgatcgtc aattcttccc accatatgat
    37861 gctgctccag ttgtagacaa caagatatta gctgatacgc ctcatttaaa agaagcctta
    37921 aataagttag gtcataccat cagtacggaa aaaatgcagg aattgaacta cgaagcagat
    37981 aataatctag tggaaccatc agtagtagca caacgtttct tacaagaaaa ccactatttt
    38041 gaagggaagt gaagcaattg gataaaatga atgtctttca acaatttttc tattatttcc
    38101 aggaaaatgg ctcctatatc tttgcacaat ttatccgtca cttcctgatt tctatctatg
    38161 gtgtcctctt tgcagcagtt gtcgggattc cggttgggat catgatttct aggagacgta
    38221 aattagcaaa ctgggtgatt cgtttggcga atatcatcca gacgatccct tctctagcca
    38281 tgatctccat tttaatcatt ggattaggcc tgggagtcaa tgtcgtgatt gttactgtat
    38341 ttttgtattc cctcttaccg atcatcaaga atacgtatac cgggatgatc caagtagaca
    38401 aaaacatcct tgatgtcggc aaaggcatgg ggatgactgc tagacaacga ctattcatgg
    38461 tagagttacc actctctgtt tctgttatca tggcaggcat aagaaacgcg ttagttgttg
    38521 ccatcgggat aaccgcgatc ggtgcattcg tcggtgctgg tggtcttgga gatatcatta
    38581 tccgaggaac caatgcaaca gagggcacat cgattatttt agcaggtgca ttgccaactg
    38641 cattaatggc gatcatcacg gactggcttt taggtatttt ggaaagacgg ctagatccag
    38701 ccagccgtac atcacgctga tgttattgta aaaaacaaaa gcttctgcga ccatttgttg
    38761 gtcgcaaagg cttttttagt tctgtcattt ttaaggcaat aatccactct gattcttccc
    38821 tccttatggg taaagagttg cttttcttag ctatttatcc attccgtata ctccaacttc
    38881 atactgccaa cgggttcata tctgcatcca ccgactaagt ttttttctta tccgctaagg
    38941 cattttgcca atccattttc ccatcactcg tcatggaatt cataccacat gatctctaaa
    39001 tactcgtctg cttgttcttt gaattgggca tcaattctta catcctttgg cgtggctttc
    39061 atttaagcct tttccatcag tcctcgattg ttcgatgcct tcagctgcat catcaaaact
    39121 ggtgactgcc gtatcggtaa cttcgatcat cttttataca atcgggaact tgccatcctc
    39181 atccttggct aaaaacatat ctagccattc cagcttctcg ccatgggagg tttgcggttc
    39241 ctaaaaccgg tacacgattc ttatggactg catccgtcgc aactagactt ggcgatacga
    39301 tgatccttca ccagaagagc acccccgtaa accaattgat cgatatattg ccaataagaa
    39361 aagacattgg cgtaaaagat atcgatccct cgtgatgcat tgccgttgat actgctattc
    39421 atggccgaaa gcgccacgac tttaacttct tccgtttgag tgacattcat gttcggcaat
    39481 gtcgctttgg atacccgctc tgctaaagga acgacactgg tattgtattt cgtatctgta
    39541 tcttttccaa aagaccatgc caacgaatcc tctgggaacc aataagacga ctccggttga
    39601 ttgtccattg cttttttcaa catacttttg gctgtttctc gataaacgac atttccaatt
    39661 gtcttcgcct cactttggcg ataatcgatg gtcgttgcca ctgccaacac taacaacgat
    39721 gctccaattt tactgatttt ccttgtcaca cttcttcctc cttgttttac ataaacccgt
    39781 caacattcca ttcctaaact caaacaatga cccttcaaag gtggagatat aggaacaaat
    39841 taaagaaatc atgcaataaa tccaagaagg ttggttttag aacaaaaaaa gagtctcctt
    39901 tttggggaga ctctttgtta actatctaaa aaggttggct cttctcactt cactcataat
    39961 gtgtccaggc agaataaatt tctacaactt tattttcctc atcaactttg taaaccactc
    40021 gatgctgtct attcagcctg cgtgagtatc tgccttcatg tgtaggtctc agcttttcaa
    40081 aagactgcgt gggcatatat ggatcttcct tcaatgtctg aatcacttct tcaaattgtt
    40141 tctttaaatt cgtttgcttg atttttctta aatccacttt cgcagagttt ttgattgcga
    40201 ctgtatagtt actcatcaaa gagcttccca atcaatatca tcgatattcg taaagctact
    40261 gtcgtctttt tcgcggtctc gtactttatc taaagtacct gtttgctcaa gaaaaagggt
    40321 ttcttgaatc gctctccagt cttccagccc aatcaataca gcattgtttc cactacgatc
    40381 actaataatc tcgatttcag tatgattttc atttacctct ttcaataact gataaaaatt
    40441 tttacgagca gtgctaggat tcatcgttgt attagccatt gtctctcctc ctttttctga
    40501 tataattata gcgtacttga ttaagtacgt caaaaaaata caataaagca cttaaattca
    40561 gcaaactttt cctctttttt acatagagaa aaagacccct ttttagggag gatctttgtc
    40621 cacgctcact tcttactcct ttacgactag cggtagccaa acgtaaagta attggtaaca
    40681 acgtatcatc tttagcaaag gtcgaatcca aagcctgtga gtctatgcaa caacaccaat
    40741 acacgaaggt tcctcgttgc agtgtgactt gattgatatc ttgaataacc tgaggatacg
    40801 cacgaaagat ttgtaacggt tattcaaaac tgattttggc cgtcaagtct tctactgcct
    40861 caaaaatgat tgcctctttc gtctcctgtc attcatgttc actatcgatt tgatacttgc
    40921 ttagaccgtt gggtttgtga atcaccaatt aaaaatggca ctgagttatg gtaacaaaaa
    40981 atggtcactt gacgaggctg agattcctac ttatgaagac acatggcact taagagtcga
    41041 ggtaaaggaa gaagctgaaa atataggagg ctgtacatta tttggcaaag gattcggaaa
    41101 ttatgatcaa gacatcaaaa tcaaaatgtt ttacagctaa ataggaagaa taataacatt
    41161 tcatacgctg ttcccatctt taatggctta tagagggcac ctttagctga gaataagaca
    41221 aaacagaccc tctagcttca attaagagct agagggtctg tttttgattt taaaacttgg
    41281 tttatttctt caaatggtag ctataggtgc tgacaacgat attttcacgc caagacaatc
    41341 gcaatcttaa ccgcaaactt gtttggttat ggagaatatt ctgccagcgt ctgtttggta
    41401 caaattgagg aatcaacaca gtagttgtat agttttgttt cgctgcattt ttacttacga
    41461 tatctacata acggataatt ggattttcaa tcgagcgata agaagagtgg acgattgaaa
    41521 aacggacatc tgggaaatgc tttttaaatt ctgcttcgat ttctttttct ttttcgacat
    41581 cttcgtctag cgaaacatgc atcgcaacga cataatcacc gatcgaacgt gcgtaattca
    41641 acgcaccgat atttacccgt gtcacattcc cgactaatac aagaacggta ttcccgtcat
    41701 aatcatgtag ttttgcatca ttttctaaac gaagctgttc tgctactttt ttataatgat
    41761 catggatctt ataaaaaatg aacataacga ttggcataat gatgaagaaa ggccaaatgt
    41821 cccctaaccg atagacaaac aaaatcgcaa tgattgcata tgagatgaac gcacccgtaa
    41881 tattggcaat cgatttgctt aaccatcttt ttccttcttt tttccatttg acgaccatcc
    41941 ctgtttgaga taaagcaaat ggaatgaaca cgccgataga gtataacgga atcaagcgtt
    42001 ctgttgagcc atggaaaata aacagcaata cgatcgaacc tagcgctagt gtgataatcc
    42061 cattggaata gcctagtcga tccccacgat cttgatacat gtgaggcatg aatttatctt
    42121 ttgccagatt gtatgcaaga actggaaaag cagaaaaacc ggtgttagca gcaactgcta
    42181 aaatcagcgc tgtagcaaat tgaaggacgt aatacaaaat accatgtcca aagacagctt
    42241 taccaatttg agacaaaaca gtgacttctt tttcaggaac gatcccatac caatagttga
    42301 taaatgtgat ccccacgaag aagaaaccta agatcaatgc catcatcgtc aatgtagcag
    42361 cggcattttt tgctcttggc ttcttaaaga atggtacagc attactaatc gcctccaccc
    42421 cagttaaaga ggaagaacca gaagaaaatg ctcgtaaaat caaagcgata gaaacacccg
    42481 gcacagccgc tcctggaagt gcagtagctt gcaatggcac agcacctgtc acgatattat
    42541 ataaaccaac aacgatcagt aacgagatca caaggataaa tgtgtacacc ggaaataata
    42601 ggaacgatgc agattccctc agcccacgaa gattcagcat catcagtagc aaaacgattg
    42661 tcacagaaat agcgacttga tgaccgtaga gtgctggcac cgctgatgtg atcgcttcag
    42721 cacctgctga gacagatact gctactgtca acatgtagtc gatcaataaa gagccaccgg
    42781 aaatcaagcc agcattcttt ccaagattct cactgctgac gacatatgct cccccgccat
    42841 gaggataagc atggatgatt tgacgatatg atagcgttaa tgaaatcaat aaaataataa
    42901 caaaagcggc aatgggtagc gaataccaga tggctgcagc agacaaagcg actaaaacga
    42961 caacgatttg ttccgtacca tatgcaatag atgataacgc atcagacgac aataatgcca
    43021 aagcagcaaa tcgggttaat ttgtgctcat cattttcagc agattttaaa ggcttaccta
    43081 ctaacaacct ttttaagtaa tccaatttct aaactccttt acacacaatt ttacttcaag
    43141 ttttatagtc gaaaatttat agtaaacaag atgttatagt acgctcactg accttaaaag
    43201 tcaataaact ttaagaaaat aattggaaaa aaacagaaaa atatcaagcg ctttttcaaa
    43261 cgctttaata cagcaaaaat ctaacatttt tttatgggct ttttctttga attctataat
    43321 aaaaggatag aacttgactt agaaaatcaa gttctatcct ttttgatatc ttaacttttg
    43381 gaagagagat gtaaggctgc tttctaccca tcattcttac tactcgaagc atagcgcagt
    43441 tgtcacaacc tcttgataaa cggtgtttaa aagcttgcat ctgcggtaat aagcccaacc
    43501 aaattaaaaa cataagaagc tgtttttatt tggtcgttct tattacttga tgcagagcag
    43561 cttttttaca acctcttggt acacggtgtt caatcagctg accctcggta ttagctcaaa
    43621 aaaacggcaa aatatgagga actatttttc catttttttc tctaatactc aggtcaaaac
    43681 actgatttca caacctcttt ttttaatgaa aaatatggct ctcatgcgcc gcttctttga
    43741 atttcgtcat tttttcttcg atgaacggat agattgccgt tcctacgatt ccaaagacaa
    43801 caaacaaacc gatcatgatc cagattgctt tcgtagcatt tggccaataa attcctcctg
    43861 cagattcccg taacaagttt accgcatgag taaatggcaa gaacggattg atcatttgga
    43921 agaatttccc tgagacttgg atcggatagt tcccgcctcc gccagagatc gacagtacta
    43981 agatgataat agcgatccct ttaccgacat tatcgaataa tgcagccaag acatagacga
    44041 tcatcatgaa cgctaaggcg atcaagacag caaatgacac gctgtagacc ggattttgga
    44101 catctactcc tagtaagaaa atattcccta atgtaacgat caagctttgc gcaatcgcca
    44161 ttgtcaaaaa tgtcagcata cgagcaacga attgttctcg tttcgtaaaa ttgtttcggt
    44221 ctttctttcc taaatagtat tctgtcgtag caacacttga aagaagaaca gcgccgaccc
    44281 acagacatag tgcagtataa aacggggtgc tagctgaacc gttatttgcg attggataaa
    44341 gcgcattggt ttgtagctca acaggttgtg taaagaagtc actttctttt gttgcgtcta
    44401 gtttcaataa tttgatgact tcgcctagat cgacttcttg ttcgcctttt cggatcgcgt
    44461 ctgctgcttt ttggatacca gctttgatat ttggccagtc attgttgatc aagtccgcag
    44521 cagcgttcac agctgtttcg acttctggca ttttttcatt tactgttgtc aatgtaccgg
    44581 tgatttcatt ttttagtgct ggccaatcat tttggataaa atcagcggcc aatcctaatt
    44641 tttgctgaat gatcggtaat tcattgttgt ataggtctgc cccttcattg atgccattga
    44701 cgatggtatc catatttcca ttcagcattg tattggcatc atggatttct tgtccaattg
    44761 caggcatttc tttttgatat ttttctaaaa ttgccactgc attcgttaca gttgctttcg
    44821 ttgagctgag aagcgaatca aagtcgatct ggtccgcttt gctcagtgcg ccttgtgcag
    44881 tcccaatcgt atcgatgact tggtctagta gagaagtgac agtggaactg atttgacctg
    44941 tattgatacc agcaatgatg ccattgatat tattaacaac ggcatcaatt tgatctagca
    45001 attcatcaat agccttcgtg tcttcatttt gaacagctgt gttcaaagca gataggcggt
    45061 tttgcagatt gcttagcaag ggttttaatt gatttagctg atcaataatt ggctgtaatt
    45121 tcgtatttcc agcacttgtt tgcaatttag tcaggaactc gaccagttta tcgattgcat
    45181 tttgttgttg agatattccg tcaataaagc cgttcaatac attgctgatg tttttctttt
    45241 catcatcagt cagcttacca tcttcaatag ctgttcttac agtatctatt aacgaacctg
    45301 tcgttgttgc cacttgtcca attgacttca atgtaacatc tacactgttt gtgatgcttg
    45361 gtatcgcatc tttcagctga gaggcacctg attttgtttg gtttgctaga tcacttgcct
    45421 gattttctaa atttttcaca tctggcaaga tatcttgtac ttgttggatg atcgtcaagc
    45481 cttgttttgc ttcatcgatt ccttggttca ttgtctcttg gatcgaagca aaatcgccgt
    45541 cgatctctgc tagttgctta ccggcatttt ggatctctgg gattttttct tgcaaatcaa
    45601 ggatgatttt tgcttgatct ttcaactcag gcattttgtc attcaatgca acgacttttt
    45661 cacccatctc atctacttta gggatgtaat ccacgaattc atttgcttta ttcagttttt
    45721 ccttgatttc aggtagttga ctttgtaatt cgaggacttg ttttgtgtag ccgtcgatcg
    45781 tgtccaaatt ttcatctgtt gataaaatca tatctttaac tttattgata cttactaggt
    45841 tcgtatcgat gtcgtaacct atctcattga atactttcaa taaagtcgaa ctggctgttt
    45901 tgataaattc atttgtgatt tgcgattgga tcgaacttgc tcctttatcg gtgattttcg
    45961 gtgcaatcgc attgattttt tcatttaccg tatattcgat ttttggtttc ttgatatcac
    46021 cactagtaaa actcagcaga tcttctgaga aatcctttgg caagtaaata cctgcgtaat
    46081 actttccgga acggactccg tcttctaatt gttcttttga atcaacgaat tgccatccta
    46141 attgtttatt tttatgaagc gattcgatga cttgctcccc aatggccact tctttacctt
    46201 gaaactctgc tggcttgtct gcactgtaaa cagcaattgg cagctctcca gtatttccat
    46261 atggatccca taatgctttg atgttaaacc acgcatatag cgaagggatg atcatgatcg
    46321 cgatgatcag aagtgttgca atcgggtttt tgaaaatccg tttccagtca agcaaatata
    46381 atcgaagcgt atttttgatt gactgcatag ttctccccct tcttcttttt tcagctgaat
    46441 tactttacca tgtatttcat acttttttca atcggctttt ttcaaaaaag tcgaattttt
    46501 ggacatatca cggcaattgt ctcattgtaa tctcttacat attgagaaat aattaaggat
    46561 tacagccttt actttatcat gaaataaaag ggaaagataa ttttatgcca aagtttttat
    46621 ctattatacg tatacgcaaa aaagctaaaa cattttttgt ctttgcccct acaaaaatgt
    46681 ttcagctttt ttcgtttttt attcctgtat ttcttatttt tcgaatgaca ggatgatttt
    46741 cccagcagga ggatgtcctt caactagctg atgtgcttgg atgacatgct taagtgaagc
    46801 agacagattc attgaaataa aaacatgaaa ggctcctttt tggtaggctt tgattatgcc
    46861 agcaaaggct tctgcatcta gatattcctt tcttggaaca aacgattcat aaaagccttc
    46921 ctttttttgc cgcaaatcta gatctgggag gtcatttaat gcaacatacc tgccgcctgg
    46981 tttcatgatc tggatgccgg tttctccttt gatacttcct ttcgtcgcat cgattacaag
    47041 gtctgcctga tcagcaaatt gcagacctgg atttgtttta tcataagcag caaatgctga
    47101 agctcctaat ttttttacct tttcctcatt tttactggat gcactggtta agattcggat
    47161 tcccttttca tggagaagct ggatcagact tgacccgacc gcacctgatg ctcctagtat
    47221 cattacagta tctgtcggct ggatttccgt caaatggttg attagattat aagccgtaat
    47281 tcctggcgtg accattccgg ctgcttcttc ccaactcatt ttatctggaa tttttgctac
    47341 ttttgctgat ggcaacacga cttcttctcc gtaagtcccg ccgactgcat ggacagctac
    47401 tcgatcacct acatgaacag ttgtgacatc tgaaccaatc tctgtcacaa taccagcgcc
    47461 atcattacct ggtacataag ggaatttcaa tgttcgtatc tctttcattt ctcctaaacg
    47521 caaggcaaca tcataaggat tcacactgaa tgcttttatt tctacacgaa catgtccaga
    47581 accgacttct ctgggatgag catcgatttc ttcaaaaaca tcttgtgctt ctccgtaacc
    47641 gttaattgca aatcttcgca tggtcaccat ctcccttttt ctttaagttt acgtttatgc
    47701 ctttattttg acaaaaaaaa tgtcttttcg ttttggcgca tgattcaaaa caaaatagtt
    47761 gtcttttttc gcgtaacgtg ttaaattatt tatctaaaag cgtaaagatt acgatttacg
    47821 ctaataaaaa ataattggag gaatttgttg atggctaaaa aatcaaagat tgccaaagca
    47881 aaaaaacagc aagaaacgat tgaacgctat gctgctttac gccaggaatt aaaagcgcaa
    47941 aaagattacg aaggcttgcg taaacttcct ttagatgctc gtccagaacg attgaaaaat
    48001 cgtgatttaa tcgatggacg ccctcgcgga tacatgcgta aattcgggat gtcccgtata
    48061 aaattccgtg aattagcaca taaaggtttg attccaggag taaaaaaagc cagctggtaa
    48121 tccaagtctg gctgtaatgt aaaggaggag ggcgcatgga ccactccttg gatcatcaaa
    48181 aaagatacgt tcgcttcttc tagcagaaat gctggtacta gaagcagacg tatctttttt
    48241 tacgcacagc tggtcctttt ttatatgtaa aggctgcatg gttacttcgc tatatttatt
    48301 tagtaacagg agtcagtgct tcgataccca cactaatttt caatgctcgg tcaacttctg
    48361 ccataatggg tcgatccaaa tggcagacct tttctttcaa tcttgattta tcgatcgttc
    48421 tgatctgctc caacaggata acggagtctc tttctattcc tgttccagta gctttgatcc
    48481 caatgtgagt cggaagctta ggtttagcca tttttgccgt tatggccgca acaatgatcg
    48541 tcgggctaaa atggttacct agattatttt gaataaccaa aactgggcgt gttccgcctt
    48601 gctccgatcc cacgactgga gaaagatcag caaaataaat atctcctctt tttaccatag
    48661 gatcctcctt attcttgata ttctcttggt atccgctgtc ctaatccgca agccacttca
    48721 tagtggatcg tacctaattg atctgctata tcctgcatag tgatttcttt gtctgcattt
    48781 ttgccgataa gcgtcacttt tgttccgaca ggaaattctt gaggcaatcg gatcatcagt
    48841 tgatccatac atacacgccc aatcgtttca cagtagttcc cttctaccaa tagagaaaaa
    48901 ccctgcatct ttcgtggcca gccatctgca taaccgatcg gtatcgttcc gatccactct
    48961 gcttcaggag tgatatatgt ttctccatag ccgattcctt cacctgctgg gagttttttg
    49021 acttgaatca attcagaaac cagctcaagt gctggctgca atggataaac ttccggtagt
    49081 gcgtgcccag agggattaag tccgtacatt gctactccat aacgaatcat attgccgatt
    49141 gtttcatcat gccataaagc cgttgcacta ttgctgacat gtacatatcg aggaagttcg
    49201 ggaagttttt tcagcacttc tataaagcgt tctttttgca gattccaata tgtatcatct
    49261 gcctgatctg ctgtagaaaa atgggtaaat aaaccttccc attctaatac acgactttct
    49321 ttgatgaatg cagctgcctc ttttacttct tcaggtgtac aaaatccgat acgacccatc
    49381 ccagtgtctg cttttagatg aatcgaaaga ggcgtctcta gcttactttt ctctaaaaca
    49441 gcagctgctg cttgcaacca ttcccgtgta cctgcagtta ctgataaatc atgagcaaca
    49501 gctacagtca gtgcgtcgta aggaaccata ttcaagataa ggatgggctg agtaaatcca
    49561 gcttctctca actcgactcc ttcatctaaa tttgaaacac aaaagcccga agctccacct
    49621 gcaactgctg cctcagctgt ttcaatagct ccatgaccgt atccgttcgc ttttactact
    49681 gcaaacaatt cttttccttg cggaagtctt gcaacttcat ttgctacatt ttcagttata
    49741 gcttttttat gtatcactgc tttcgttggg cggtgccaag cgacaaccat cgtttttcct
    49801 tctttctagt cttccaaaat aatttgtgca aaagctgttt catttgtatg agtaatactg
    49861 acccatacat tgccttcatg aggtgaacgc gtcacaacag gctgaccatt ttcatttttt
    49921 aaaatctcga catcttgaaa ggtaactttt ccgatcccag ttccccaagc tttagagaaa
    49981 gcctctttgc aagcgtaacg cccgcctaaa aattctactt ggcgatgaaa aggcaaggac
    50041 tggaatagtt tcatttcatc agaggtcaag attcgtgcaa tgaattttgg tttttcttcg
    50101 atcagccgag tgatcctagg aagttcgact gcatcgatcc caattccttt tatcatatgc
    50161 ttttctcttt tcaataaatt tagaacactc tcattctacc aaattataac cattttgcca
    50221 caaataagtc cacaaataac aaaaatgaaa gtttctctta aaaaagagaa actttcattt
    50281 tatgtttaat cgttatttga acggattaca aatccgcgtt ttttatcatt gtgacgttta
    50341 ccgccatctt tttggctgtt attatttttg ctataaccgc cttttgattt gtttccacga
    50401 taattaccgc cgtcacgacg attacggttg tttccgccac cacgtttgcc atttttattg
    50461 tatccttttt tctgcattgg caatgggcgt tctggtgtga tcttcactgg cactgcatca
    50521 gctggatctt ttgctgtcgt ttttagcaaa agtgcaacta aatcttgtgc agaatattct
    50581 tctaataatt tatctgctgt ttgcaggtat ttatctagac cattttctgc aagttttgtt
    50641 tcgatttgtt caactgcagc acctaattga cctttgaatg cttctttttc ggttggtgga
    50701 cgtaaagtcg tcatccgttt tttcgtcaag ttttcaatca catgcaagta gtccatttca
    50761 tttggtgtaa cgaaagtcac agacataccg ccttttccgg cacggcctgt acgtccgata
    50821 cggtgaacgt agctttctgg atcttgaggg atatcatagt tgtacacatg agtgacacct
    50881 gaaatatcta atccacgtgc agcaacgtct gtcgctacaa ggatatctaa atgaccgctc
    50941 ttaaatgaac gcaatacgct cattcgtttt tgttgagaca aatcgccatg gattccttct
    51001 gcacggtaac cgcgtgcttc taatccgcgt gccaattcgt ctacacggcg ttttgtacgt
    51061 ccgaagacga ttgttaattc aggtgtttga acatcaaaca agcgagtcat gatatcgaat
    51121 ttttcgtatt cttttgcgcg gacatagtat tgatcgatca agtcagctgt catttctttc
    51181 gctttgatct tcacatgatg tggatttttc atgaatttaa cgccaatatt cttgatagct
    51241 ggcggcatcg ttgctgagaa aagtaatgtt tgtctttgat caggaacttg tgaaatgatt
    51301 ttttcgatat cttccaagaa gcccatattc aacatttcat ctgcttcatc caaaactaat
    51361 gtttgaacgg ttccaagttt caatgtgtgg cggttaatgt ggtcaagcat acgtcctggt
    51421 gtacccacaa cgatatgtgg acgatctttc aagccacgga tctgacggcc aatatctgct
    51481 ccgccgtaaa cggcttgtac acgtactttt ttgtcgcgcc ctaaacgata taattcttct
    51541 tgtgtttgga tagctaattc acgtgttggt gcgataacta aaccttgtaa ttcgtgacga
    51601 tctggatcga ttttttctaa cattggtaat ccaaatgcag ctgttttccc tgtacctgtc
    51661 tgtgcttgtc caataacgtc ttttccagct aatgctagtg gaatcgtttc tgcttggata
    51721 ggcgttgctt cttcaaaacc agcgcgttcg actgatttca acaattctgg tgataattct
    51781 agttctttaa atttcaaatg aatcctccta tatggttgtt aactgaggat gtagcttttt
    51841 tgctaccccg tcgatttgtt cggtcaaagt gtattgtttc tttttgacag tggccacgca
    51901 cgtttttatc cattcttagc ccctgtggca ccaccgaaaa atcactttgc gaagtttagg
    51961 cattagaaaa gccaaacggg tcgcgttcag ctttatcatt attatgacat ctggcttcaa
    52021 caagttgttt ctatggcaaa accagtccaa tttctcttca tctttcggca ataggttatt
    52081 ctaacatagt ttttcgtttt ctgcaagttt acagattttt atttcatttg ctgaagttgt
    52141 tctaccactt caaataagcc catgctgttg ctgcctttta gcataatact atctgttgct
    52201 tcaagatttt gtttgatagc atcaatcaat ttattctttt ctttttcaaa atagaacaca
    52261 ggtagttggg ggtatttttg gttcaattct tcttttagtg catgcatttg ttccccataa
    52321 aggaaaatca tggcgaattt ctcaggatcg atatgttcac tcatttgcgc atgcatattt
    52381 aaagactctt tccctaattc cagcatatct gctaacacag cgattctgcg tccttttgtt
    52441 ggcagatttt taaaagtatc taataccagt cccatggctg ttggatttgc attgtagaca
    52501 tcgctcaaaa tagctgcacc atttcctgcc gttagccatt cggttctgtt ttgagtgata
    52561 gaaacatggg cgagcccttg gcgtatctca ggtatggaca gtccaaagaa gcgcccgatc
    52621 gtatacgcaa tcaaagcatt cgttacatta taacttccag gcagcgggat cgtaaatgtt
    52681 tctccttcta ctacaaaatc ggtttgtgct tttccttttg ctgtcacttc cgcatgaaca
    52741 tcgccatttc caataccgaa tgttgtgact gtttgtacta atgttgccgt taaaggtgtc
    52801 agcaacggtt catctgcggg cacaacgagt aatccgtctg atacaagtcc gtcagtgatt
    52861 tccattttgg ctttagcgat tccttctcgc gacccaagat tttctatgtg tgcttcaccg
    52921 atcattgtga ttgctgcagc ttttggttga gccataagtg aaagttccgt gatttcattg
    52981 gcgtgatcca tgcccatttc caaaacgatc ttttctgtat cttggggcat gtgcaaaatc
    53041 gtgtaaggaa gaccaatatt attgttgtaa tttccttgcg tcttatatgt tttgtatttt
    53101 tcagacaaga cagacgcggt tagatctttc gtggtggttt taccattgct tcctgtaatt
    53161 gcaacgactt ctgcgcctac tttttccaag taataggttg ccaattgctg catggctttc
    53221 aaaggatctg ctacaggaat aactggaaaa ttttttggag cttgttcaac aggcaagctc
    53281 caaaatgaag cagatgctcc gtttgtgatc gcttgctgga taaattcatg tccatctcgc
    53341 gtaccagcaa gaggaacgaa taaacttcct tcggttatct tccggctgtc aaattcgaca
    53401 ttggtaatct ttgtcagtgc agagcattcg ctgcccacgg cttgtgcaat ttcttgtatt
    53461 gttagattca ttgagatctc tcctaaaaaa ggttgtgaaa aaagcgtttg gcctaaacgg
    53521 cgggttcgca tgactctcac ttccaagtaa atttctctat tcgtatttag aagctagaat
    53581 gaacgcggtt acgcaatcac tccagcctct tctttatttc tctttattca tttttttcaa
    53641 aaaaagattg acgctgatgg tatcgattca ttcctaactg gatcagttct tcaatcaaat
    53701 caccgtattt caagcccata ttttcccata agagtgggta catactgaac tccgtaaatc
    53761 ctggcataga gtttaattca ttcaggaata attcattttt atttgtcaaa aagaaatcgc
    53821 accggctcaa tccgcttcca cctaacatcg tgtaagctaa cttcgcgtac tcttgcgctt
    53881 tttgataaac ttcttctggc acttcggctg gaatctgcat ttcgatttta ttattgatat
    53941 attttgcttc ataatcatag aatgctacgt cttttacgac ttcaccaggc aaagtcgtcc
    54001 gaacgtcttc atttcctaat acagcaactt cgatttcgcg cgcttcaatt ccttgttcaa
    54061 cgattgctcg agaatcatac tgataggctg ttgctaaagc attttgcagc tcttctcggt
    54121 tttctgcttt tgtaatgccg acactagaac ccatattcgc cggtttgaca aacatcggat
    54181 aaagcaaaga accttcacat tgatcaaata cttttttagg attttctttc cattgattct
    54241 taagtactgg tacataagga acttgcggca caccagcagc ttgtaaaata tacttggtca
    54301 tgattttatc catggcacat gcactggtca atacgcctgc gccgacataa ggcatattca
    54361 atgtctctaa gaagccttgg atcgttccat cttccccgtt tggcccatgt aaaactggaa
    54421 aaacgatggc tccttcttct ttgatttcgc ccggattgat cacttttcct gtaaagcctt
    54481 cctctgtctg tccacttggg tcccatgaaa gatgcaagac atctttgctg gcaggttttt
    54541 ctgttagtaa tggacctttg acccattgtc cttcttttgt aataaatacg agttgaactt
    54601 ggtaataatt ataataaatg gcatttaaaa ctgaaaatgc ggaaagaatg gacacttcat
    54661 gctctgcgct gcgtccgcca tatagtaaag taatcttcaa aagattgtcc tcctgtattc
    54721 taaatcgttc agtgatttac tctcatattt tagcacaaaa caaaaataaa cgaataactt
    54781 atttataaaa aaacgagcca gcttctaaaa gaacgagcgt catttatttt ttgtcagcta
    54841 gattatttgt cccgtttttt ataaaaagtt atgataaaga aaaggaggga tgatgatggc
    54901 gcaaacaaat ccacgcagcc gcttggcaga aatcattcga gtgttcatca attacaatgt
    54961 agtgccaaac tttgtacagc aaaaaaatcc cgaacaggta aaaaaagcct ttgaagaact
    55021 aggccccacc ttcatcaaga taggacagat gctttctgtt cgagaagatt tgctttcttc
    55081 tgccttcact caaacattca aaacattaca agatagtgta cctagcgaca cattttcaac
    55141 ggtcaaaaag acgatcgaaa cagaactttc tctttcatta tcagatatat tcgatgattt
    55201 ttcaaaatcg ccttttgcct ctgcttctat ggggcaggct catcgtgcca agctgaagaa
    55261 cggtgacgcg gtagtcgtta agatccaaca ccctaacatc gccgaagaga tccgacttga
    55321 tcttcaacta tttgaacgag cgatcccttt gatcaaatac attcctgaaa ctagcgtagt
    55381 tgatctaaaa ggggtactcc aagaagtaaa acgttccttg atcaatgaaa tggacttctt
    55441 aaaagaaagt caaaatggcg aacaattcta tcaaaagaac aatggctgga aagagatccg
    55501 cagccccaag atttatgacg ctttttgttc gaaaaaagtg atcgtgatgg aagaaatgtc
    55561 tggtaaaaac ttgaatcatt taatgaacgc cgaaaacaaa actgaaactt ttatcactgg
    55621 aattcaaaac aagcagctga aacaagaagt tgcaaagctt ttagttgaga actttatgaa
    55681 acaggtgttt gatgatggtt ttttccatgc tgatcctcat ccagggaatc tgctattcca
    55741 tgtactaaca aaagaagaac agacacaagc tagccgaaaa actgaaacag tccatgaaaa
    55801 agagttcggt tcctttgctt ttcgagcctc cacttctgca gaagaccctg ttgcgccata
    55861 taccatcaac tacatcgatt ttggtatgat ggggcatctt tctgcaggac ttaggcaaaa
    55921 actcacacaa gccgttttag cgctgtatac gaaagatgct tatcgaatcg aaaaagcggt
    55981 ccttcgactt tgtcagcaag aaggctcgtt tgatgaaagt cggttccacc aagaactgac
    56041 aagttttctt gaacagtatt atgattctcc tattgatgaa atcaacttgc aagaagtatt
    56101 cacacatgtc gtgacgattt gtcatcaaaa taatctgcag ttcgatcgtg atatcacgct
    56161 tttgctgaaa gcatttggga cattagaagg ggtgatccgc gtcctcgatc ctgaagtttc
    56221 cctcatggaa gtcgcttcgc cttttgctca acattatttt ctcactcatc tggatgtaga
    56281 agatacattg aaacaaagcg gattagactt attggaaggg atgaaggctg cgcctaagat
    56341 ccctcaacag ctccaccatc tattagaaat gtggacttcg ggacaaggaa aagtcaattt
    56401 ggaattgaaa aagcaagaca aattattgag caggatcgaa agtatgatca atcggcttgt
    56461 tttcggtatg attttagctg cattgatcgt agggtcctcc ctgcttgtcc aagcagcacc
    56521 tgtagaaaac gcagaggtcg tttccttatt agggatattt acgtatgcta ttgccgcatt
    56581 cgtcattatt tttttggcaa tcgatgcatt gatccaaatg tataagaaga ggaaaaagta
    56641 gaaaaaggtt gtgacagaaa gaatcagctt caagaaataa gaaggggttt tacataaatt
    56701 gtttctcaaa tttttgtgaa aattcggctt atttttcgaa gaggctactt ctgaaacacc
    56761 gagaataaaa aatgtgaaaa agctgttctg cacccagtaa taagaacaat caaaaaggga
    56821 acatggaaca catttatcct gtttccctgt tccctttttg atgtattcga gcataaataa
    56881 aataccttcg ttacctacta tcttctgtac ttggtaaacc ttagtcaatt gcctttttta
    56941 acgcttgacg aaaagacata tgatctttct ccgtaaaggg ttttggaccc tttgtcctta
    57001 tgccagcttt tcttaacttt ccactatgat aacgttgttc tagaagcgtg ccgatattcg
    57061 cttcgtcaaa ttcttttcca gagtgatgga acactcgcac acccttcggc aatacaagtg
    57121 aagctaaacc ataatcttgc gtgatcaaaa agtcattctt cttaaccata ccgacgattt
    57181 tataatccgc actatcagct ccgcggtcta cgtagacgat ttggacatga ttagcgtatt
    57241 ctttggtcgt ataatggtcc acactggtca cgataaccac gtcaagacca taggattctc
    57301 ctaattttat gacatcttct tttactggag aaccatctcc atcaatgaat agcctcattc
    57361 tttttcgcct tcttccataa atgataaaca gttactaatc cgctcagtaa aagaccaaca
    57421 ttgatccatt ttgccaagat aagcagttgt acactttcta tcggcactga ttcatggttc
    57481 agcgtgatca ataatagatg gattggctct cccatttctt ttgctaaaac aaacaaaagc
    57541 gtcatcaata aaaactggtg gatcacactc cctttatttt gagtagtata cttgacaacg
    57601 atccggcgat ccattggaat agctgtatca gtttgataat attccttatt tagccagctg
    57661 cctgttacta cgtgccagaa actaagcaga aagacaccta tcgcaaaacc atttggaatc
    57721 aagaggacca ataaagaaga actcagtcca agcaattgca tagccaatgg agaccatgtt
    57781 aaacgtttca aaatccatgg tccgactatt tttgctgcaa tcaagcctgc tgcataaggc
    57841 aaatacataa aaatcgaaag tttttctaat ccgtacagta tgccaacata aaaactgcta
    57901 aataaaaaaa ggaaattccc tagcattcca tttagaaaag taaacagatt tagccagctt
    57961 ggcaacttcc attccttctt ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc
    58021 aagaaaccat aaatagcaag atcaaaaagc ctttcatcga ataacaatcg ggcatttctc
    58081 aaaagaaaca ataagccaaa gaatatcaaa aataaaacaa gctctcgcgt agctaccaga
    58141 tttcttttga tgtctttgaa atccagttcg taacgcggat aatgactaac cgtatggtag
    58201 gccatgacgt aaaaaagtgt ataaatagta aagcttgctg caatctgagt cggtgcaggc
    58261 aactcaatcg cttgatacag gagaaatagc cagatagctg caaataagta ctttttccca
    58321 tccatattga taaaaccttg cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca
    58381 agccatgctg cactcagccc tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat
    58441 acgcctaata ttccaagcaa tgaaccagca ccaccaatca gcagagcgat catcagcaac
    58501 gtgtaactat ctagaccaaa tcgtagactg ttgactaaaa atagtccagt catccgaaag
    58561 gcataaaaca gcgtaaatgg caaaatcgtc agccaattat gcgagtctgt tagtcccaaa
    58621 aataaaagat aaggaataaa aataaccgtg ttacttaaga taaagggcgt tgtttgtgta
    58681 aaattacgcc caaatcgttt gagtttttcg aacatgacaa tggctcctta aacagtctta
    58741 taactaatct gctcttcgat tttagcatta aaggggaaat aaaccaattt tacagaatat
    58801 attcttgtaa aaaaaaatac agaaagaagc ataagcttct ttctgcattt ttacggtact
    58861 tgattactca agcagacaag cccttctcca caaaagtctt gcccgacacc cctgcatccg
    58921 gcaggtgata aagatagaaa tcaattatcc tgtgcctcca aaatcaatta ggagctctgt
    58981 aaaccagagt ccttctcgac ccgtcgcata aatttacttt aacacagttc ctatgtttat
    59041 gcgagagctt ttttcatttt ataatcgacc atttgaaatc cttgaccgat atctgaaata
    59101 agttcttctg taatctcaaa tccttcatgc ttataaacct cgattgcttg atgattcccc
    59161 tgattcactc gcaaaaagat ctctctttcc tttgtttgcg cagcaagttc ttcataccag
    59221 cgaaaaactg aagaagtcaa tccttgacca cgtactttat ttagcaaata taatttactt
    59281 aaataaattt ttccatttat ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt
    59341 tttaataaaa agtaggacac accttctgtc agttcttttt gaatctgttt ttttgactga
    59401 taagttttca gcatgtaatc cacttgtttt tgaccaatga tcggcacaaa aacttccggc
    59461 catacttctt gtatgactgc catcagctca tccagttcat tttctgttac ttttattttt
    59521 tccatacacc cacgtccttt tttacattat aaatgacctt gcgcttgatg gaataaaaag
    59581 agcatgatca tgctatacac acccaccgac cacggtttca agaaaatcac aatctgcata
    59641 aattccctaa aagataattt cgtcaaacca gctactagac agatcaagtc gtctggcgca
    59701 aagggaagca gcaaggtaac aactagtact tttttaatat ctttcgtttt tcttgtcagc
    59761 aattcatcaa atttctgata cttttttggc gataaaataa gctggacaaa ggctacgcca
    59821 tagtaacgga caagcaaaaa tccgattgct tccccaatca ctaggccaat acaactatat
    59881 aaaagcccaa atccattgcc aaacatcagc atgcccgcta ctgaggagat ccctcccggc
    59941 aaaataggaa tgatcacttg gatcacttgc aacagaacaa atatcatcac tgcatacttg
    60001 ccaaattgct ggataaacgc ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa
    60061 gcataaagaa tcaacacaaa gaaaaaaaac agaccaataa tgggcatgat ctgaagacat
    60121 ttttgtaaaa ctgatccttt tttagacatc tatattcccc acatcttttt tcatttatta
    60181 aaactgcctc ct
//
LOCUS       DAYYFU010000012        58482 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000012 DAYYFU010000000
VERSION     DAYYFU010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.012"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..257)
                     /locus_tag="ACHA34_000876"
     CDS             complement(<1..257)
                     /locus_tag="ACHA34_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791541.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAF"
     gene            complement(273..923)
                     /locus_tag="ACHA34_000877"
     CDS             complement(273..923)
                     /locus_tag="ACHA34_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791542.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(934..2028)
                     /locus_tag="ACHA34_000878"
     CDS             complement(934..2028)
                     /locus_tag="ACHA34_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM8791543.1"
                     /translation="MILLISSGSEEEEIDCSPTEIVASTISLTNQGMETNGLQIAKEV
                     QKHIPNTTLEGLAGMLGNFQQESSMSPTAIERPNDPLSGHGIAQWTAGRTTALKNFAK
                     ERNKEWSDLGVQVEFLIHELNTTEKASQVALQAKSVEEATEQWQVKFERAGIPMMGNR
                     ITFANQWYAKLGKNDSDPVASITLENGANAGSKDLTDYCLETTGTSSSSDSNVLNVAK
                     SLIGYFHYSQPQRAQFGSVENPDKNGYADCSSFVWLVLTKAGYKTPANVGWFTGSMTS
                     DARGSKQYLQEIPESEAKAGDIIIVNLGSGVGNDGHTAVLAEDWHGLSTKVIEQGGMN
                     TNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2125..2997)
                     /locus_tag="ACHA34_000879"
     CDS             complement(2125..2997)
                     /locus_tag="ACHA34_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8791544.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3019..3939)
                     /locus_tag="ACHA34_000880"
     CDS             complement(3019..3939)
                     /locus_tag="ACHA34_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791545.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3939..4208)
                     /locus_tag="ACHA34_000881"
     CDS             complement(3939..4208)
                     /locus_tag="ACHA34_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791546.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4205..4585)
                     /locus_tag="ACHA34_000882"
     CDS             complement(4205..4585)
                     /locus_tag="ACHA34_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFM8791547.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5052..6515)
                     /locus_tag="ACHA34_000883"
     CDS             complement(5052..6515)
                     /locus_tag="ACHA34_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8791548.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6579..9596)
                     /locus_tag="ACHA34_000884"
     CDS             complement(6579..9596)
                     /locus_tag="ACHA34_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8791549.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9747..10586)
                     /locus_tag="ACHA34_000885"
     CDS             complement(9747..10586)
                     /locus_tag="ACHA34_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HFM8791550.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10731..10976
                     /locus_tag="ACHA34_000886"
     CDS             10731..10976
                     /locus_tag="ACHA34_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791551.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11039..11461
                     /locus_tag="ACHA34_000887"
     CDS             11039..11461
                     /locus_tag="ACHA34_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791552.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11555..11872)
                     /locus_tag="ACHA34_000888"
     CDS             complement(11555..11872)
                     /locus_tag="ACHA34_000888"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791553.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11899..12105)
                     /locus_tag="ACHA34_000889"
     CDS             complement(11899..12105)
                     /locus_tag="ACHA34_000889"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791554.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12086..12268)
                     /locus_tag="ACHA34_000890"
     CDS             complement(12086..12268)
                     /locus_tag="ACHA34_000890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791555.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12282..12632)
                     /locus_tag="ACHA34_000891"
     CDS             complement(12282..12632)
                     /locus_tag="ACHA34_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791556.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12658..12960)
                     /locus_tag="ACHA34_000892"
     CDS             complement(12658..12960)
                     /locus_tag="ACHA34_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791557.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12963..13196)
                     /locus_tag="ACHA34_000893"
     CDS             complement(12963..13196)
                     /locus_tag="ACHA34_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791558.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14100..14630)
                     /locus_tag="ACHA34_000894"
     CDS             complement(14100..14630)
                     /locus_tag="ACHA34_000894"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791559.1"
                     /translation="MKMTPNHLRHICCEVTLFNRQRWKLSNRIVRRIIVDSTGKSTVI
                     LTKKLATKQDFQLFFSQVKTIFFDGKIIANRGCSNQDLMVSNIVFSRMKRRQDLLIQA
                     RKAYKVFKKQEKQSRGKQNEQIMNKKCIEVLNGKNDHSDTKKKEGRHENYPILLVCPN
                     CFSCGFVGRIGFRTWN"
     gene            complement(14627..15742)
                     /locus_tag="ACHA34_000895"
     CDS             complement(14627..15742)
                     /locus_tag="ACHA34_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8791560.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(15748..16146)
                     /locus_tag="ACHA34_000896"
     CDS             complement(15748..16146)
                     /locus_tag="ACHA34_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8791561.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
     gene            complement(17175..17561)
                     /locus_tag="ACHA34_000897"
     CDS             complement(17175..17561)
                     /locus_tag="ACHA34_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM8791562.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(17558..18079)
                     /locus_tag="ACHA34_000898"
     CDS             complement(17558..18079)
                     /locus_tag="ACHA34_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM8791563.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(18081..19115)
                     /gene="sppA"
                     /locus_tag="ACHA34_000899"
     CDS             complement(18081..19115)
                     /gene="sppA"
                     /locus_tag="ACHA34_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM8791564.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(19148..20563)
                     /locus_tag="ACHA34_000900"
     CDS             complement(19148..20563)
                     /locus_tag="ACHA34_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFM8791565.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(20532..22685)
                     /locus_tag="ACHA34_000901"
     CDS             complement(20532..22685)
                     /locus_tag="ACHA34_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFM8791566.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            22943..23179
                     /locus_tag="ACHA34_000902"
     CDS             22943..23179
                     /locus_tag="ACHA34_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFM8791567.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            23198..23413
                     /locus_tag="ACHA34_000903"
     CDS             23198..23413
                     /locus_tag="ACHA34_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFM8791568.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(24116..24868)
                     /locus_tag="ACHA34_000904"
     CDS             complement(24116..24868)
                     /locus_tag="ACHA34_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8791569.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(24883..26130)
                     /locus_tag="ACHA34_000905"
     CDS             complement(24883..26130)
                     /locus_tag="ACHA34_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8791570.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(26206..26352)
                     /locus_tag="ACHA34_000906"
     CDS             complement(26206..26352)
                     /locus_tag="ACHA34_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFM8791571.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(26456..26767)
                     /locus_tag="ACHA34_000907"
     CDS             complement(26456..26767)
                     /locus_tag="ACHA34_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8791572.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(26769..26966)
                     /locus_tag="ACHA34_000908"
     CDS             complement(26769..26966)
                     /locus_tag="ACHA34_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8791573.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(27360..29087)
                     /locus_tag="ACHA34_000909"
     CDS             complement(27360..29087)
                     /locus_tag="ACHA34_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFM8791574.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(29080..30273)
                     /locus_tag="ACHA34_000910"
     CDS             complement(29080..30273)
                     /locus_tag="ACHA34_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8791575.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(30460..31104)
                     /locus_tag="ACHA34_000911"
     CDS             complement(30460..31104)
                     /locus_tag="ACHA34_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8791576.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(31198..31332)
                     /locus_tag="ACHA34_000912"
     CDS             complement(31198..31332)
                     /locus_tag="ACHA34_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFM8791577.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(31334..33466)
                     /gene="feoB"
                     /locus_tag="ACHA34_000913"
     CDS             complement(31334..33466)
                     /gene="feoB"
                     /locus_tag="ACHA34_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFM8791578.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(33463..33936)
                     /locus_tag="ACHA34_000914"
     CDS             complement(33463..33936)
                     /locus_tag="ACHA34_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFM8791579.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            34337..34561
                     /gene="nrdH"
                     /locus_tag="ACHA34_000915"
     CDS             34337..34561
                     /gene="nrdH"
                     /locus_tag="ACHA34_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFM8791580.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            34720..36879
                     /gene="nrdE"
                     /locus_tag="ACHA34_000916"
     CDS             34720..36879
                     /gene="nrdE"
                     /locus_tag="ACHA34_000916"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFM8791581.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            36929..37894
                     /gene="nrdF"
                     /locus_tag="ACHA34_000917"
     CDS             36929..37894
                     /gene="nrdF"
                     /locus_tag="ACHA34_000917"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFM8791582.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(37983..39122)
                     /locus_tag="ACHA34_000918"
     CDS             complement(37983..39122)
                     /locus_tag="ACHA34_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8791583.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(39431..40144)
                     /locus_tag="ACHA34_000919"
     CDS             complement(39431..40144)
                     /locus_tag="ACHA34_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFM8791584.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(40398..41099)
                     /gene="nagB"
                     /locus_tag="ACHA34_000920"
     CDS             complement(40398..41099)
                     /gene="nagB"
                     /locus_tag="ACHA34_000920"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFM8791585.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            41333..42865
                     /locus_tag="ACHA34_000921"
     CDS             41333..42865
                     /locus_tag="ACHA34_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8791586.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(43056..44258)
                     /locus_tag="ACHA34_000922"
     CDS             complement(43056..44258)
                     /locus_tag="ACHA34_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8791587.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(44340..45029)
                     /locus_tag="ACHA34_000923"
     CDS             complement(44340..45029)
                     /locus_tag="ACHA34_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8791588.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(45135..45503)
                     /gene="rplL"
                     /locus_tag="ACHA34_000924"
     CDS             complement(45135..45503)
                     /gene="rplL"
                     /locus_tag="ACHA34_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFM8791589.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(45556..46059)
                     /gene="rplJ"
                     /locus_tag="ACHA34_000925"
     CDS             complement(45556..46059)
                     /gene="rplJ"
                     /locus_tag="ACHA34_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFM8791590.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(46263..46390)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(46469..47158)
                     /gene="rplA"
                     /locus_tag="ACHA34_000926"
     CDS             complement(46469..47158)
                     /gene="rplA"
                     /locus_tag="ACHA34_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFM8791591.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(47262..47684)
                     /gene="rplK"
                     /locus_tag="ACHA34_000927"
     CDS             complement(47262..47684)
                     /gene="rplK"
                     /locus_tag="ACHA34_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFM8791592.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            48027..48695
                     /gene="sdaAB"
                     /locus_tag="ACHA34_000928"
     CDS             48027..48695
                     /gene="sdaAB"
                     /locus_tag="ACHA34_000928"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFM8791593.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            48708..49580
                     /gene="sdaAA"
                     /locus_tag="ACHA34_000929"
     CDS             48708..49580
                     /gene="sdaAA"
                     /locus_tag="ACHA34_000929"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFM8791594.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(49671..50771)
                     /locus_tag="ACHA34_000930"
                     /pseudo
     CDS             complement(49671..50771)
                     /locus_tag="ACHA34_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(50863..51750)
                     /locus_tag="ACHA34_000931"
     CDS             complement(50863..51750)
                     /locus_tag="ACHA34_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8791595.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(51827..52372)
                     /gene="nusG"
                     /locus_tag="ACHA34_000932"
     CDS             complement(51827..52372)
                     /gene="nusG"
                     /locus_tag="ACHA34_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFM8791596.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(52473..52643)
                     /gene="secE"
                     /locus_tag="ACHA34_000933"
     CDS             complement(52473..52643)
                     /gene="secE"
                     /locus_tag="ACHA34_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFM8791597.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(52667..52819)
                     /gene="rpmG"
                     /locus_tag="ACHA34_000934"
     CDS             complement(52667..52819)
                     /gene="rpmG"
                     /locus_tag="ACHA34_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8791598.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            52981..53625
                     /gene="cbpA"
                     /locus_tag="ACHA34_000935"
     CDS             52981..53625
                     /gene="cbpA"
                     /locus_tag="ACHA34_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFM8791599.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(53681..54184)
                     /locus_tag="ACHA34_000936"
     CDS             complement(53681..54184)
                     /locus_tag="ACHA34_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8791600.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(54197..54243)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            54781..55746
                     /locus_tag="ACHA34_000937"
     CDS             54781..55746
                     /locus_tag="ACHA34_000937"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8791601.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(55845..57017)
                     /locus_tag="ACHA34_000938"
     CDS             complement(55845..57017)
                     /locus_tag="ACHA34_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8791602.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(57004..57129)
                     /locus_tag="ACHA34_000939"
                     /pseudo
     CDS             complement(57004..57129)
                     /locus_tag="ACHA34_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            57237..58316
                     /locus_tag="ACHA34_000940"
     CDS             57237..58316
                     /locus_tag="ACHA34_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8791603.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT        17423 a        11801 c         9708 g        19550 t
ORIGIN      
        1 aaaaaggcaa gtgcttcttt gtaggcattt tgactgtatt tttttgataa agcatgacaa
       61 atttcttgat aaaatagttg actgcctacc gaataccgat tattgtgtat ccgccacaaa
      121 acgaacacac tgatttgaat cgacgggcgt aaaaaagaaa aattctttcc ataagaaaaa
      181 gaacgtcctt cttgggtttc ttttgcttgt tctgttggta cttcttgtac ctctcttatt
      241 gattcttgtc tctccataac cttctccttt tcttattctt ctaaaagctg ttgattaaag
      301 ctacttccca aattttgcat aaacgtgatt ttctttgacg ctggatcaaa ggttacgtca
      361 aaaatattca tggcattatt ccaggaacta tcaccaattt tattgcgata cgacaccaca
      421 acgagccctt ggatagtcgc tttttgtgtg ctttttaaat aaacagaaca tttctctaat
      481 tcagaagaca ccccgtttga atcaccatag gaaacgcctt ctttgaaata ttgcttcatc
      541 agtttgtcag aaagatatgg tgcacaattg ttttttcgtt cttgaaaaga atcaggctca
      601 taattaaaca gaacctcgaa cgcttctgtc actgtttttt gaaaggtttg aatgtttcct
      661 ttttctgttg aatcattgtt tttcaattct tcatttgctt tttttaacgt cgtgacctcg
      721 ctttctactt gttgaagttg ttctttgagt gacgttacaa ggtcttcctg tcctttcttc
      781 tcttcttgga acttgtttat ctccaccgtc agtttctctt cctttgctgt tagctgtagc
      841 ttttcatgaa taagaaaaaa tgtactgcct acaccaaata agaacaatcc gcctacacaa
      901 acataaataa atcgtttctt catttgacac ctcttatttc tttatcggtc gagcaaaaca
      961 aatatcacca ccattaagca aatacccaaa ggataaattg acttgccctt cacctacacc
     1021 gttagtattc attccccctt gttcgattac tttcgtagaa agtccatgcc aatcttccgc
     1081 taaaacagct gtgtgtccat catttcccac accagaacct aagttcacaa tgataatatc
     1141 gccagctttt gcctcgctct caggtatttc ttgtaagtat tgcttacttc ctcttgcgtc
     1201 gctcgtcatt gagccggtaa accacccaac gttcgcaggc gttttatacc ctgctttcgt
     1261 taaaacaagc cacacaaagc tcgaacaatc ggcatagcca tttttatctg gattttcgac
     1321 actaccaaat tgtgcccgtt gcggttgtga ataatgaaaa taaccaatca gactttttgc
     1381 tacgtttaat acattactat cggaagaact gcttgttcct gttgtttcta gacaataatc
     1441 ggtcaagtct ttacttcctg cgtttgcccc attttctaat gtaattgaag cgacgggatc
     1501 actgtcattc tttcctagtt ttgcgtacca ttgattcgca aaagtaatac gattccccat
     1561 cattggtatc cctgcacgct caaatttcac ttgccactgt tctgttgctt cctctacgct
     1621 ttttgcttgt aatgcaactt gtgaagcttt ttctgttgtg tttagttcat gaatcaaaaa
     1681 ctcaacttgt acacctaaat ctgaccactc tttgtttcgc tctttggcaa aatttttcaa
     1741 agcagtcgtt cgccctgccg tccattgtgc aatcccatgc ccagataatg gatcgtttgg
     1801 tcgttcaatc gctgtgggac tcatactcga ctcttgttga aaattgccta acatacccgc
     1861 tagaccctct aatgtcgtat ttggaatatg tttttgtact tcctttgcaa tttgaagccc
     1921 gtttgtctcc atgccttggt tcgttaatga gatagtactt gcgacaattt cggttggact
     1981 acagtcgatt tcttcctctt cagaaccact actaatcaaa agtatcataa acagaaaacc
     2041 aaacatacat agtaatagtg tgggtagaca accgaaccca caccataaaa gaagtttttt
     2101 cttcatgttt gatccttcat tcgcttagtt cattttttgt gttgcttttc ttttgcgcca
     2161 aaaagcaaaa ccgcttcctg aagccaataa tccaccaagt gctgtcgcaa cgagagacac
     2221 ttcttcgcca gtcttaggca atactttcat ttttccatca gaccctttca ccgcaatatt
     2281 tccgcttgga agttgttcga ccttgtctga aatgggcgtc aagccatttc ctgattcttt
     2341 ataagcgaca ccatcaacga cggagacaat tttttctcct gaagctgttt caattggtgt
     2401 acttggtgtt ggaatatggg ttaattcaga cgcattattg gtttcaattt gaacattttg
     2461 tccacctgtt tgttggctgc caatacttcc atctggtttt actacgacgt cctgtactgg
     2521 aggcgtagtg ttcgtccccc cattattggt aggtggtgtc gttggaggag tcgtatttcc
     2581 ttcattgcct cctggattcg acggcttttc tggtgtctct gtattaccac catttcctgg
     2641 gttttcctca acgggtggaa cttcaggtgt tccttcccct ccgccagtat ctggattttc
     2701 tggtacttca ggcgtttctg tgcctccacc tgtatctgga ttttcttccg ttggtggaat
     2761 ctctggggtt tcaggagttt ctggtatttc gggaatttct ggggtttctt ctcctccatc
     2821 tgttggtggt aaaacaggtt cttcaggaac ttcaggtgtt tcttccgttg gtggaataat
     2881 ttctgtcggt ggtacacttg gatcacctgg tgacgtagta tctgcatacg cactggttcc
     2941 taccatacct agcaatacag ttgcactaaa taaacttcct aaaatcattt ttttcatgtg
     3001 tttttcctct tctcttattt attgatttat ttcaaaatca tctagttcgg gaagcgtgga
     3061 aactagataa tttttttcct gttcttccaa ttgaaaagac actttttttg tttcttgtgt
     3121 ctttttctcg gtttccaatg taatgacata cgcaatttgc caatgcttgc cttctttctt
     3181 catttcccag gataaaatac ttttgacttt ttccttttga ggactgattt gtttcgcaac
     3241 tttttccgtt agaaacgatt gaagcttttt cctataccgt tcttctgcca tttcacccga
     3301 ataataattc gtaataaaat aacgactaaa tgtatcaatt tgtggttgtt ttgtcgcaag
     3361 ttcttccgca aatgcaatgc gttcttgtaa ctggtgctcc tttttcgtta gttgttcaat
     3421 ctctctttgc gttgaaagtt gcataataaa aagaccacaa gatacaacga cgataaatcc
     3481 aaacaaaagc cattgaaaag acattttttt cttctgaaga gagccgttgc tttgtgtaaa
     3541 cgctgtttct acagaaagtg gtgttttctt ttcctttttg atttttgttg gtcgacgttc
     3601 gttcttttgg tctttcttta cttgataaaa cttcggtaat tcggaattta aggtttgaat
     3661 aaaggtttgt gcttcttcac ttaattgccc attttctgtt aaagattgtt caatcaatga
     3721 aatcaaatga aactcttcta ctggaaaatc atacgagcct tcaaatactg gaatttcatg
     3781 gttttctgtg tagatttcca aggtacacca gccaatttcc gctcgtgcca atagctcttc
     3841 attttctctt ttcaattgaa taaatgcctt atctaatgca atttttttat ctgttagtaa
     3901 agacatgtta tcaacttctg taaatcgaat catcatcatt agtcaaacac ttcttccacc
     3961 acttctaccg catacaccac ttgaataggg ttatttaaag aaaatttcac ccgatattct
     4021 tgattgttca aactgacgat atagctatcc tcatcttcag cataataggt atattcgttg
     4081 cctttgactt gaaaatattt ttggatcgct tgtctttgtt gccattcgcc aatctttgga
     4141 aaataggcga aatgatagcc aaataaaacg cctatcccta ataaaacaat tcctgttact
     4201 ataatcatct ggtttcctcc tgtacctgtt tatcttctat tgactctttt gtcggtaagc
     4261 aataaagaac cagtaacaca agagagccaa tcgttgggac aaaatagaaa aaaataaatg
     4321 cccaatgaaa acctgcgtct cgtaaacgtc gaacgctgag agaaagagac gggacaagaa
     4381 ctacgaaaca atacacaatc aatacgccta tcaaacaacc aattattgtt tcagaagcat
     4441 tcaacgctcc tgctagtcca atcgctaagc ctaggccaaa cccaatgagg gcattcatta
     4501 acgcaacgag ccaatactct tttcttgtgg ctttcccttt gtaattaaag ctattctgcc
     4561 aaaatttttt ataatttgtg atcatatctt tgtcctttct aatgtttatt tttgattcta
     4621 gtttcttctg ttttactaac aatgaaactt gccataaaaa aggtggccaa tacaccacca
     4681 gctagaaatc cacttacaaa taacaaaatt ttttcctcct ttctaaaaaa tttcgccata
     4741 aaaaggcaaa gtatgaacac gataaatacc atgtacatac tttgcctttt tatgcaatat
     4801 atgttaactc tccaagcaat tactgatttt taaattcttt ataaataaaa taaaaatcgt
     4861 ttgagtaaag aaataagtca atgaagaccc aaacgataaa aaatattcct aatactatat
     4921 ttaataaact acgttctaga tagatacgag atttcaaatt aaaaaacgaa aatgagagat
     4981 acaaatatat caaatattta aaaagtaacc acattttcta taccactcct tatcagaaac
     5041 agccttattc tttagaataa gactgtttct gggttgatta atttgtcttt tgttaaacta
     5101 ccatctaagg caagttctaa atgtaaatgt ggtcctgtac tatttcctgt tgaacccaca
     5161 taaccaataa tttgtccttg ttcaactgtt tgtcctacgt tcacgcaata ttcctgttgg
     5221 tgcgcataaa gagccacact tccgtcttca tgctcaattg tgacataatt tcctgacaaa
     5281 gtatgatacg aagcttctat gaccgttcct gccttgctcg catagattgg ttctccttga
     5341 ttggctgcca tgtctaatcc tctatgaaat tcttctcctc gccaaccgaa ggaactagaa
     5401 attgaataat tttttagagg taccatatag ccttctttgc ttagttgtgg accagctact
     5461 tctttatcat atttgattaa gtcataggtt tcgattaacc catttaattt tttatcgtag
     5521 ctagtgtctg ttgcgtatct tcctgttaaa aatttagtcg cttcctgata ggtttttgcc
     5581 ttacttttcc aaacaccatc ataaaaagag gtatttccaa ctaagccttc ttttaacagt
     5641 ttggcatagt cttttaagct ttcttcatag ctctcatatt gccgaaaagt tgcttgggtc
     5701 gtataaagtg tcccattgcc taaatcttct tgtgtcgcaa aagagacgcc ctttccgtta
     5761 tgtgtgcctt tgattccaaa tagattgtag tttggtgcct gagccaattg actttgtccg
     5821 cttgcggatt ctaaaatggc ttgcgcgatc ataacagaag cgtataaatc attttcttgt
     5881 ccaattttac gagcagattc accgatttta cggataaacg tttctacaga cgcgtctttt
     5941 tcaaaatgaa tagtcccttc gttatcttgt gtgctggaag ggactaattc ttgtatcggt
     6001 gcgcttggtg cttggtggta gttttttgtc tcttctgaac cgattgattg tgcctgcggt
     6061 acgtcattca taggtacagt cacactctgt tcaggtgcct tttgtgggcg ttccgttttt
     6121 gttggcggtt ccgcttctgt ttcggtactt ggtttttgag gagtctgtga gctttcatca
     6181 gaaggttgct catttggttc ttctgacggt tcctcctcag gttgttgatt gtcttctggt
     6241 ttgtctggtt gattcggtgt ttctggttgt tcttcagaac ctgttggtgg agcgttcatt
     6301 tctggattat ctggtgtatc tattgtatct tcagtcggtg tttctggttg attttcttct
     6361 ccagtggaat ttgttgtcgt ttcttcagtg atcgttgcag attgcgaatt tgtcagttga
     6421 ttgttgcctt gttcattagc caaaaccgct ggcgtatacc caaatacttg acctactaat
     6481 aacaaagcag ataaacattt actcgttttt ttcacaagat tgcctccatt cttcaatcaa
     6541 tcatctagag acagctgatt gctgtctcta gtcatacttt aattgctttg tttcatttta
     6601 cgacgaacca gaacagcccc cacaccaaca aaaagaaaca gtcccaatgc aattaacaca
     6661 tcgttcactg tttctcctgt ttttggtaag ctttgttttg gtgtgctcgt cggcgtttgt
     6721 ttgacagggg ttgtcggcgt ttctttttta atgccttttg ctgtcaatgt ttgtttttca
     6781 ttcttcaaat caaagtgacg tgcttcttcc tctttatggt ctttatcctt atagacaata
     6841 tgagttgcga ccaatttctc cccgtctttt aacgtgcctg ctttgtagtc aaaggatact
     6901 ttaaactctt cagacgcttt agaaacttta cgtgcagttt ctaccgtacc aacgactgta
     6961 tcttttccat ttttatcaat cttatggaat tgagtcaccc agtatttggt ttcccctacc
     7021 gtagattctg gaaattcttg cgatacatga tcgatcaact cttggtcttt gtctggatca
     7081 aatgtttggt cgccattcac tgtaataaag cgtgtttcga tttttggttc tttcgcagtc
     7141 actgtttgat ctttattggt taaatcaaaa tgacgagcga attcttcttc ctcttctttg
     7201 tcgttataga caatgtgcgt tgcgacaatc gtgtcaccgt ctgaaaccat gtctgctttg
     7261 tagttgaacg tcacatcaaa tgtttctttt tcatcggtca cttttcgtgt ttcttttacc
     7321 gtatctaata cagtgacttt tccatcggca gtaattttat ggaattgcgt cacccaatat
     7381 ttctctgtcc caaccgcaga tttagggaat acttgggaca ctttatccac taattcttgg
     7441 tcttttttcg ggttgaaatc ttttccacct gtcgttgtca caaacaaagt agaaatttct
     7501 ggttggtctt cttctggaac cacgatttct tcgtttttca cagtaattgg ttgtacttct
     7561 ccgtctttga ccgttactaa aatcgtttct gtttgtcctg acgcttgccc ataatgctca
     7621 gggaacacaa tttccgtaaa tttatacgta ccgtctggta attttagttt ttctggttct
     7681 tcttttgttt catacacaaa ggacacattt ttattatgtg cagtcatacc ttcaatcagg
     7741 aatttcgctc ctggtaattg tagatgattg attgaatcga ctttattgaa gtctacttca
     7801 cccgttttta acaagttgtc aatcacatct aattcaaacg taagcacagg cgtcgtgtta
     7861 tcgccatagg ccacagagaa cgtggtttgt tcttcagaag gttgacggta gttgtctggc
     7921 acattttttt caatcaattg gtatttgttt tgatcgccat tgtagaattt tttgtttgcc
     7981 ttccagtgtc cgtctttgtc agtttttact gaatccaaga cttctccttt tttcacaagg
     8041 actgtttctt tatctggcgc agtaatatct gttttggcga taatatcaaa ttcgatattt
     8101 tctgcaggaa cggtatcata aacaggtttg ccttgattcc aggattcttc cgcattatag
     8161 acttctttga ctttctttcc ttctggaatg aactgttgta agtcattttt tgcagttaac
     8221 gtcacttctt tgtttggttc aatcgtaatc gtatgagttt gtttgtctag aatataccca
     8281 gctggtgctt tgacttcttt gacatccaat ttattttgaa catattttcc gtctttttca
     8341 tagaatgcag ggatttcttt taaggcgatt tcccctttgg catccgttgt atattgtttt
     8401 gtttctcccg tatcatgacg tttgatttca tacgtcacat ttggaacttt tttccctgtg
     8461 acagcgtcta gtttggttag tttgaagtcg ccatttttta gaactttaat agttagtcca
     8521 gctctattag gatctggcac ataaagcttt cctagatttt gagcaacagg atctacatag
     8581 gcaaaacttg aacctacccc tctagaattt ggatccagtg aagaaacaga aattcgtccg
     8641 tcatttgagt tttgatttgc ttttataaac aaactatttc ctgattttct tacttgaacg
     8701 ttagctgtgt tttcaacaac cgacccatcc cattgagaaa acacaccttt tgtatcctta
     8761 acttctactt cttcccctac tttaaccgtc actgtttggc tatcaaaaga aggatcttca
     8821 agtaaattgt ttatttttct atctacagtc tgtttgaaag cttcataatt agattgtcca
     8881 atagttccat aattaacaaa tgaaccacct aaaacttccc aaatatatgt ttgtgctaat
     8941 acatagttat ccattgaacc actagaatag tagccaaaat aaccaattaa agaaatcttt
     9001 tttctaatag cacttgtcgt aattaaattt tcgagtggtg tcacatcata ttgaataccc
     9061 gcactcgtga ttgcgccttc ttgaatgcaa aaaacatctt ctccgtcaat ttgccagcga
     9121 accatgtaat catgagtata cattgttcct tcaggagtaa ttcgagtcat attcacatct
     9181 ttacgaccat tttcagtcaa tgttagattg gtagcacgtg tctgtgcatt cttttccaag
     9241 attgcttgcc attcttcaga ttcttgcgcc aatcgttctt gttcagaaaa agaagtcgct
     9301 ttttgaatat cttcaatggt tataaagtat ttctcataac cttcagggac ctcttgagtc
     9361 attacattgg gcggagaaac atcttctgtc gtgtcctgcg tagttgtctc ttctgttgtt
     9421 tcacttgtgg tttcagtact gatcgcttct tcactacttt ttgattcttc agtcttcatt
     9481 acttcttcag tagaatttga ttcatttatc gtttctgcaa atgccgttac agatcctaac
     9541 atgggattga gaaatgtaac gaaaattgcc aaccaagttg ccattctttt aaacatattt
     9601 ttttcgttcc tttcatttta tttttcagtc ttctaaagta atttcttatg gacttcgaca
     9661 ccgaaacatt tttatcctcc tcaaaacaaa aaaagactag aacttataat tctagtcttc
     9721 atcaaccact tacttttctt ttatttttag tagaaatcta cagtgtgttt ttgtgacccg
     9781 tcactgtaaa taataacatc caccataaaa cctttgttgt accactgcga atttggatcg
     9841 ctaacctctg cattagccca atcataggct tcatctacag tgttaaatac ctttccacta
     9901 ttaccaatct ctccaactac aattgttggc tcttcaggtg tcgttgtgcc accatttccg
     9961 ccttgatcgc ctccaccagg attgcttggt tcttcaggcg ttgttgttcc tccacctgtg
    10021 cttgggttag aaggagttgt ttctcctcca gtatttccac cattacctgg ttgtgtaggt
    10081 gtttgagtag tgccaccatt gttttctggt gtacttggcg ttgttgtacc accagttgtt
    10141 cctgtttgat ctccagctgg tgttgttggt gtcgttgtat ttggtgtcgt ttgtgtattt
    10201 gaagtcgttc cttgtcctac tggttgattc ggatcaactt tgtctttctc ttgaatcact
    10261 gtttgagcca cagtttcacc tgcgctgttt tgtacacttg ctacattgcc attaaatact
    10321 aatttatttc ctgcatagat taaatccaca tttgtaatct tattcatgtc cgctaatttt
    10381 tggacagtaa tatttgttgc ttgactgatt gcgcttaggg tatctcccca gacaattgtg
    10441 tattcattcc ctttaatttc tgctttgatt tgttccgccg aacgaggtgt ccaaactttg
    10501 ttttctgctg cttcagcatt cgttcccaca gcaagactaa ctcctccgac tacgattcct
    10561 agtgccaata ctttcaacat tttcattgtt tttcgcctcc gatttgaata aatttattta
    10621 caaaaacata gtaccaaact tatgtctatc agtcgatata ttttttgtag atttttttat
    10681 tttttgatat actaactttg gtactaaaat aactaaaagg aatgtcacac atgcaactta
    10741 ataatttaaa tgaaatacat gattgggctc aaaataattt acttaatcgt aaacaagctg
    10801 ccaaaatcac tggacaaaac tatgatgctt tttctagctc tttacgtaga aataatatca
    10861 aaccatttct agaaataggc gacgaacctg gtccttccat cattcgctta tatctaagag
    10921 aagatattga aaaatacgca aaacagctcg aagaaaaaag acagaaacgt caatgaggcg
    10981 ttttctgtct tggatttaca agtaaaacaa taataaacac ttttacaacg gaggttctat
    11041 gaataagaaa tacattgttt cagttggttc aatcgtactg attgtactcg ctattattgc
    11101 tttttctgta tccaacaaaa cagaaacaca gccacaaaat acggttcaat cgactactac
    11161 aagtcaatct agcgatcatt ccgtctatcc ttcactaaat agtttaaaag aaacgcatgg
    11221 acaagattta attgatcttg gttcttctca aattactact gtcttatttg aaaagaaaaa
    11281 tattgcgttt attactccta aagaaggaac tgaccttgaa ttttctatca aagtaacaaa
    11341 taccaaagaa aatttattct ccattcctga cattcaatat acccctaaca agttaaagat
    11401 tggggatacg tttggtttgg ctaaagtaaa tgaacaatac tttgcttact cactgaaata
    11461 agtttctaat agcaagagga aaaaccctct tgttattttt atcacataat cattttataa
    11521 aaacgcttcg aaaatatcaa catttttgaa gaaactattt ttctaatttc tcaatttctt
    11581 ccaagggatt ttcgctaatg acccatacag gcacagattg ctctcgaggc tccccttctt
    11641 ctaaccacca acctgcataa tgaacttctt cctcctcagc attaccatga taccaaccaa
    11701 caaaggattc atctacaacg aatgaaattt cttcttctgg atcaaataat tcatttttcc
    11761 atgcttcaat tgcttgttct ttggtgtact tttgtttgtt aaatacacca caaatttcgt
    11821 catctacata aaaaatttca aaatcaaaat tactcgttcc ttttactggc atcttaactt
    11881 ttcctccttt gactcacttc aatagatata ccactcatgt tcattccatt gtttactagt
    11941 taattggagt tttatcttag ggctttgtgc actctttcca aaaacttgag cgtcgttttt
    12001 ttcttctaac caaaaaaggt aatttcttac attcggtact aaatgttttt cctgcatttc
    12061 ttcatgaaaa acatataatt ctgcttcatt tttcaaccaa ttcattattc attccctcat
    12121 tcacttcttc ataccgccat tcagatagtg agatttcttt ttgtttctcc acttgtccta
    12181 taaaaatcgc tgtattcaac aagctgatca cacttaataa taaagcaatg aaactaatgg
    12241 ttacacttac tctttcacat tttgtcattt tgtttccttc tttactcgtt tgttatttct
    12301 tgtaataggt ctaataactc cttcaccgaa atttgcaaat ggttcgcaaa aatttcaata
    12361 aaagcactcg cacacattac cagaccttca actgatatat cactgcgaca ataaaattct
    12421 ggagaaacac tgatttctga ttctttctta caattgaacg aaaaaactat ttttgaatca
    12481 aattccggta attcttccac aactatgttt tgggattcta ccaagaaagg gagtgcctgt
    12541 tctaatgctt cttctggtgt ttgagcagat agaataattg cttcttgttc ttttatttca
    12601 ttgttctcaa atactttata actttttttc attgttagtt cctctttcat tcctctctca
    12661 attagctaag ccgttactgg tacgccattc tctaaattta gtatcgctaa tccttaatgc
    12721 tcgtcgaata tcaatcattc gataacctat tgcacgaagt tctttatatc ttttagcagt
    12781 tagcgtctct aacgtcattt caggcaatgg gttcgaatca aaaatagaat tcgaaccgat
    12841 tatttgggcc aattgttgta cttgtaacag ctcttctgaa cttaattcag tgattgctcg
    12901 attgcctatc attgcataca ttttctgtcg aatatacttc ttttcttgtt tagacaacat
    12961 ttctatccac tcctattttc attatcattt gttacctcca cttcagtaaa tccacaaata
    13021 aatgaattca tattccaagt accattccct tcaactacaa cttttctcct gtcctctttc
    13081 ggatattcaa gaatcagacc gtttaccaat atgtttttca ctactactaa tttgtttgta
    13141 taccgtggaa ttgctgttcc gatatacttc actttatttc ctacgctaat tgtcatttcc
    13201 tcactcctat tcttttttat ccaaaacttt ttcctgtcct attcctgcca acgaatatac
    13261 ttcactgcat gcttaaacaa aacaaaatgt tgccctgttt tatttccgtc ttttacttgt
    13321 tccacataca gttcatattt ggtcacacct tttaaaacgc cagatacttt ttttcatcca
    13381 ttgtcacaat tagttttttc atcaatcgat tgatactgca ctgctcattt ttcttgttca
    13441 tatagtattc ctttcttttt caaaactagc ctcttttgtt tactagtttt catcaatcat
    13501 caacgattta acagattttt tctttcgcag ggtttctagc gtcagcaacc ccaaactcta
    13561 aaaaagtgcc tttgctccct tgaaaaacga gcggacgatt gtactcgtca tcatatgttt
    13621 ttcatactac ctgatccacg caaataacct ttttttgttc tgtgccacac ccatccatac
    13681 gtatcgcttg agattcacca tactttttta ggtggtttat ccttcctaag tccctgttag
    13741 acacgctgat taacttagaa agtcctgttt gtatccaatt ttcaaagatc tcccaaaaga
    13801 aataagtagg tttacgtgta tcgcttctgt tcaaagtacc tgtttgtact tgagtatttc
    13861 tactcccctc gttcaaggga ttccttccac cagctagctt ttcctttcat catcaaatgt
    13921 tcctgaaaag catttacgtt tcgtgtccgt gtgcttactt attctttttt cgatagcgat
    13981 tatccgctcg ctactcgcta ttttatactt tgtttgttag ctcaaataaa aactaacaaa
    14041 caaaaaaggc tagtttcttt ccgttttatt tttttaactg tcgataaata gttgttagtt
    14101 taattccaag ttctaaaacc aatacgccca acaaaaccac aggaaaaaca attggggcaa
    14161 acaagtaaaa tcggatagtt ttcatgtctt ccctctttct ttttggtgtc gctgtggtcg
    14221 ttcttgccgt tcaatacttc gatacatttt ttattcatta tttgctcgtt ttgttttcct
    14281 ctggactgct tttcttgctt tttgaatact ttatacgctt ttcgtgcctg aattaatagg
    14341 tcttgtcggc gtttcattcg tgagaaaaca atatttgaaa ccatcaaatc ctggttcgaa
    14401 caaccacgat tcgcaataat tttcccatca aaaaaaatag ttttcacttg agaaaaaaac
    14461 agttggaaat cttgttttgt tgctaatttt tttgtcaaaa tcactgtgct tttccctgtt
    14521 gaatctacaa tgattcttcg aacaatccga ttcgataact tccaacgttg ccgattaaac
    14581 aatgttactt cacaacaaat atgacgtaag tgattagggg tcattttcat gtgtccaatt
    14641 cctccaacca attatgcatc gaaattttgg gcaattgtga cagcccataa atctgcgttt
    14701 ccaggtcttc ataactacaa gaacgcttgg cttgaagtaa tgaatattct gcttgatctg
    14761 atagttttgt tgccattgga tctagattca attctcttac acaacattcc caaaaattca
    14821 atagcgctct aaaataaaat ccttttcgat tctgaattgc ttttccttca tgacgagcaa
    14881 ttttttcatt ttgaatacac cgttgatacg ttttgcttaa atcttctgca aatgtttccc
    14941 ctaaaattcg atactcgctt ccatcagata aaccatgtgt ttgattatac tttcgttgcc
    15001 gcttttctat aattcgttta ctgtcgtata tcatgtttag ctgctctttc gcttcacgcc
    15061 attcagctga actgcgaaca atccatgtca aaacatcttt cggaacaaac gtactttcgc
    15121 tgtaatgagc aataaactct ttttgccaat catcagccat ttcaacagga ctagtcgtat
    15181 cgatttttgt atctagattt tctgtatcta gattgttaga agaattatta aataattgtg
    15241 cagaattttc acatggattt ttttcgtcaa aatcatcgaa aatcgctaaa ttatcaacag
    15301 attcctcctc ttcttttcct tgtgcagaat ctacactggt tgagtcaaca ggacctaatg
    15361 aatacacatc attttctgtc gtatctaatt ctcctagata caaccgagct ttgtttcttt
    15421 ttcctgtttg ttgatcaaat cccatatcct ctatttctag tagctcatat tctttcaatt
    15481 cttttttgat tgtattaaca gaacgtctgc aacaattcaa tgcattggct aattcttgtt
    15541 ccgtaaattc aaaataaaca aaaccttgac tatctacttg atttcgttga atggctaaat
    15601 ctaagcgtga ccgaaaaacc atatacgcga tttttgcttt atcagacaat gagcgatatt
    15661 tttcacttgt aataaatacc tttggaaatt gatagtataa ttcattgtat ttacgagttg
    15721 cggtaaatct ttttgttgcc atcgttatta cacctcatct tgtccatata ttaagaaaga
    15781 attttggtgt tggtagacac gaacatcttc cattgtagca atcaattctt ttagataaat
    15841 tcgaccgaat tcaattgtta gtaagtcgta atcttctaat tcatttttga tttcttcaat
    15901 ttcttcttca gacacattga gtaaaatggc taattcttga ttggtataag aaatatagac
    15961 atgtttcttt tcatcaatcc gtttttcaat taaggattct tctaagtact cattcagtaa
    16021 gctataggcg tattttgctt gtgcagtcat cgcataatat tttgacgatt ttataaagaa
    16081 tagttttggc atatgaaaag ttgcttgtgt cagcacatct gttgcgcgaa atttgttttt
    16141 taccatattt tttcctccta gaaaaacacg caaaaaaagc tctgtataaa tcgaattaca
    16201 cagagtttct tcttgttgat atttaatttc taaaatacgt ttcgggtaaa aactaaacac
    16261 ttactaatat ttagattgta gaatcttcgc cttcatttat tcattttaaa gagaaaaaga
    16321 tcaagtgaaa tgtgcccaaa agtttgattc tattaatttt taaacaccat tcataatata
    16381 tttcctataa tgtaaaaagc gtcgatactt attaaagcat cgacgttatt tttatagtaa
    16441 atttatcaaa atttttgcca acattttttc cgattttttt ccctatttct atctttcctt
    16501 atctttctta gttaatatta tttgatgtta atatatcact actaatatta aaaaacattg
    16561 atttatcata ggataaagag tgatagttta atacttaaag agacatatta attttattta
    16621 aaaattgaca ttaatgactc ttaatcagcg ggtcgcgggt tcgagcccct cacggcccat
    16681 tgggtgccaa acccacggaa caggtaaatg ttggtgcgaa agcatttcga aaacatttac
    16741 gctgttcttt ttttattttt caaaataact agggcttttt atttttaact caaagtccac
    16801 tgtagccctt tggactttga gttaaaaaag aagcataatt atgaacagta aagtttctaa
    16861 tataacaata attcgagaag ctttgcaaaa tcatgctgcc aatgaactct ctaaacatat
    16921 aggattaaat ctaagtacta taaaaggtta aaaagtgaag aatatttaat agaaaaacta
    16981 aatttacttg ctgctttccc cttttccatt aatacaacaa tcagagagtg tctttttttc
    17041 gaaaaaatta gaataaataa aagacacgta gtttttttat ctacatgtct tccatcgact
    17101 acaagttatc atttctcaaa acgtatttag cttttaattg attaatcgtt tcacgttgtc
    17161 tacacattgc ctaattaaga ttttcttatt agaagcatca atcccgcaat aatacctaaa
    17221 actccaggaa ttacccacaa taatgttaaa agaaaaaatg gaataataga taaaatgagt
    17281 aaactaatac catagaccac tgttttttga ttttttctta ctaaaaaacc tattatgaaa
    17341 atcattatcg aaaaaactag ggtaattcct ccaaataaaa caacattccc gcttagattg
    17401 actaacatat ctatatcctt actggttagt tctgtagtat ttaaagtagt tgaatcaata
    17461 gaagatagaa acataccacc catcaatgct aaaagactac ataaaatccc tgttattcca
    17521 ctaattaaaa tcaaaataaa ctctgttttt ctcttattca tttaattctc tccagattaa
    17581 gtgttttaga attagaaatt ttttgaccat agttaatagc ttctaatgaa cgaataacaa
    17641 ccactgacgt gttagaaaaa taatccccaa tatgttgttt cttgggcgtg aaagcagcca
    17701 ccaaataccc aatttgaaat acaaaatttt ttaatataaa cctacatata cactcacgaa
    17761 taaagatagt ttgccaactt agctcttttt cctcaaagct cactactctt atcccaaaaa
    17821 tcattttccc aattgtttgt cctgaattca gcttagtcaa aagtacaaaa taggttaaat
    17881 aaatagctaa tgacgctata ccataaaaag taataatgga tttgtcagat tccagtccca
    17941 tgattttgta aaagagacct atagtagcat gggtgatgat gctaatacaa attaaatcaa
    18001 taagaaaagc aaaaaatcgt aaccaaaatc ccgcaaatac ataattaggc aaagtttcaa
    18061 tgccactctt cttattcatt ttatagccct ccatagtaat acattggttt tggagtatca
    18121 gaaaatccaa gtttttcaaa aactgaaatt acacgatctg atgtagttgc ctttagccct
    18181 tgccattcag caatcttatt acctaaccaa gaagaagcaa agccagttgt gtcattttta
    18241 tattctataa ccgtcgcatc agttaatttc ttctcttttt ttagggaatc taatgcatct
    18301 tcagagtaac caatttgatc caccaaccca ttttctactg cttggttacc atcataaatt
    18361 cttccatccg caagttcttt cactgcatta gtatccatat ttcttccttt tgctacaaca
    18421 ttgacaaaac gatcataagc actcatcaca aattcctgta taactttatt ctcttcctct
    18481 gaaggtttgt gttgaggtga catcatatct tttaatgcac cacttttata cgtcgcatca
    18541 gttacgccaa gtttttctaa taagccagaa taatttaaac cagaaataat gacaccaatt
    18601 gatccagttg ttgtttcttc cgtagcaaat attttatctg aaccagcaga aatgtagtac
    18661 cctccacttg ccgctgtatt tttaaatgca gtatagatag gaatatctag ctttttaatt
    18721 tttgccattt ctttagcaat ctcagcactt tcatatattc ctcccccagg tgaatttact
    18781 tctaataaca ctcccttaac cgctttgtct tcttgaattt tctttagctg tgttaaaaaa
    18841 ttctgatggt tatattgttc cctactaaaa agattgcttt cacctgtatc tgcaataact
    18901 ccattgacgg atagtttgac aatcttttta tttgatgcgc cttcttctaa cgtttcttct
    18961 gtgatttcgt tagatccgta aaaaatctta tttattcctg ttagggttgt atttgatgct
    19021 tcctctttat cttttcctgg aattagcgcg acgataatgg atagtaacaa taatccgcac
    19081 gctactccca cagcaatcca tcttctttta ttcatataaa ctcctcctat attcttagta
    19141 aaaataatta ttttttattt agaaacacat cgacaatttg ttgccaatat gttttttctc
    19201 ctacaattaa agacaactct ccagttaatc cataacgaaa cggaaacgat tgagttgttt
    19261 ttaacgttcc tttcactgca taaaaaacac ctttctcagt ggcagtagaa ttttctgaaa
    19321 tttcatctaa agttccgtcg atcgtaatcg gagccccacc tttttgatcc aatttaaaat
    19381 gaaccttcat cccttcttta atataggtac tttcattcgc aggtaaaagt gctgtaaatt
    19441 tcatttttga tttttcgtgg atcggataaa tttctgcaag cactgttcct ttggctagtt
    19501 ctgcttgtcc ctttgtttct tcatttaaat gaaccgttcc agatacagga gcttttagta
    19561 ttccttttga aagttgatcg tccaaacttt tgataactga ctggttcttc tcttgttttt
    19621 catttagctc tgtaactgtt tgttttacag ttgttattgc ttgttctagc tcttgctttc
    19681 ctttttccgt ttgactaatt ctgtcattct cagaagtagt tggtttaact aatttagctt
    19741 tttctgactg atactgttct acttcttttt ttagttgctc aatttgttca tttatgcttg
    19801 ctgaaattgt cagtttcact tgattttttt gatcatcaga aacattattt agttgttctt
    19861 gccaattctc atagtttgcc atcatttctt ttgaaaaatc ttttaatgat tgattattac
    19921 tccaagctac ctgtacttgt tgccattctt gtaatttact atctgaactc tcaatttgtt
    19981 tttctagttg tctttttgtc ttttcgtata cttctaattg cttttgatca tttaattcac
    20041 tttgcttcaa agcgtagcgg agtgattctt tttctgacaa catgctcttt aattgattac
    20101 tgtagccaaa actgtcatcc gttgaaaaca aattttttcc tttacttata ctttcaataa
    20161 atagctgagc catctttatt tgttgagtaa tacgttggtt ttcttgttct atctgagcct
    20221 tttcattctc tattaaagaa caatccaatc taaccaacac atcgtcctta tgaacaaatt
    20281 gattttcttt taggaagttt tccattattt tagcttctaa tggcacttgt aacttctcta
    20341 ttttttcggg gttcaattga gccgttgtcc gaataacaac ctctttttta gcaaaggtta
    20401 aaaacaatcc aataatacat aaaaaaagaa ttacagggta taacacccaa cggtagaagc
    20461 tatgatgttg tttagtatag atactagaat gttcgaacca attattattc tgcataaatt
    20521 gtttccctct cctagatttc ccataatttt tgatagatac catcggcaaa acgaagttca
    20581 tcgtgcgttc ctttttctac caaatgccct tcatggagaa ctaaaatttg atcacatgct
    20641 tttgctattg gtaaatgatg cgcaataaaa ataatcgttt tttcttttaa attgaggaga
    20701 taattcataa ttgcatgttc taacaaggta tctaatccac tagttgcttc atctaaaatt
    20761 aatatgtccg gattttttaa taaagcccgt gctaaggcta atcgttgtcg ttgccctcct
    20821 gaaagattat ttccaccttc ttccaacaat gtctcgtaac gtaatggctg ttgattaata
    20881 aattcattta gttggactat ctcacaaact tctattattt gttcgaaact tggtgggcta
    20941 tctaaaccga atataagatt ctcaatgatt gttccactaa agaaaaatga ctcttgaggc
    21001 aaatatgtca ctcgatcgcg taatatctga aaaggaatgt ctaagtgatt aatgtttcca
    21061 taatggatcg atccttctga tgcttcataa aaacgaacaa ataactttgc aagagttgat
    21121 tttccagaac cactaactcc aactatagcc gttttactgt gcgaaggaat tgtacatgaa
    21181 atttcgttca acgttggggc cttcatgcta tacgagaatg aaacgttttt tacagtgata
    21241 tcttgttgaa ataattgttc agaaagcata tattcaaaag actggttcga tttttctgaa
    21301 tcaatcgcga aaatttcatt taaacgatta tttgcaactt gagccgtttg cattttcact
    21361 tgtaaattaa taatgttttg taaaggatct gtcaaaaata ctagtaaagc attgtatgtg
    21421 ataagttgac ctagactaat tctattatcc ataacaaaat aagaacctag ccataaaatt
    21481 aatgcactgc taattaattg aatcaaatgt ttaattcctt gttgaatatt atcaagcgta
    21541 accgtacgaa atgatttttt cattaattta acaaattcat ggtctatacg gttatatact
    21601 ttctcttcac cgctatatgc tttaattgtt tctattcctt taagactttc tatgatacta
    21661 gaatttacag ctgcaccggc tgccatttct tccctgttcg atttttcata gcttttcacg
    21721 aaagcgaata ttgcaactac ataaaaaggc aaagagcata gagtgattaa aaacaacgta
    21781 ctattctgaa tagctaacgt aaccccaaca agcaacacca taccaatatc taaaaatatt
    21841 gataacgtag cacttgctaa agcatcaata atcttactgg catcaagaaa acgagaaata
    21901 atttcccctg attttctcgt agcaaaaaaa ttcattggta aagttaacac atgtttgaaa
    21961 tactgaagca taattgccat actcattcgc tgtcctaaaa taataagaag ataactacga
    22021 ctatattcaa acaatacccg aaataaatag acagcgatca agccaatgga aattatattt
    22081 aaagttgaac gtgcttgatt agggatgaaa aaatcaagaa taccttgaaa ataatatgag
    22141 cttcctatgc caaaaaacgt aatgaaaatc gatgctaaaa cgatgtggaa tactaatcct
    22201 ttttgtttcc aaattatagg caaaaaagaa cttaatccag ctactttttc tttaatagga
    22261 atataagttt ctctaggcat aggtaataaa agaatacctg tccattcttt ttgaaacgct
    22321 tcaatcgttt ttctattttt tccattagcg ggatctgcaa tccatagtac gtttcccttc
    22381 gttttataaa ttacaacgta gtgcatatag ctgttatcaa ccaatatatg tgcaattaat
    22441 ggtaagataa gatctctttc tttccagatc tcactatctg cttgaatagc catacaatca
    22501 aaagacaatt tttcaaatgt ctttttcaat ccaaatgctg aagtaccctc taaatcagtc
    22561 ccagataggt cccgtaattt atgaatcggg atatcagttt taaaatattt taaaatcatc
    22621 gaaaggcaag caacaccaca atctttttca tcttgttgct gcacaaaagg aaatgatttt
    22681 atcacttatc taacacaact ccttctaaaa aatttttata gaaaaattct caatatagct
    22741 ttaatacagt gaaaatattg tctcataatt ccacaaaaac tttgaaaaca gttcttgaac
    22801 actaactttt tcattcctga atcaaattta ttttggaaat tttactattc aagaataaac
    22861 ttgataagtt tcaggaagat ttttactgtt aatcaaatac gtatgttaca gtagcaccgt
    22921 aaaaaatatt atttaaagaa gcgtgtttaa acatgcaaaa tgtaaaggaa ctaagtattg
    22981 tggaaatgca caaactgttg gtggaggcaa taatgcttgg ggaaaattgg ggcaagttgt
    23041 aggtggactt accacagggg cagtaggtgg tgcaggttta ggaacagcaa tttgcggacc
    23101 agcttgcggt gttgttggag ggttatatgg agctgtcgct ggaggtgctg cagctggctg
    23161 ggatgctcga aaaaaataaa aaaggagaat aagtagtatg catattaaaa acacaaaaac
    23221 aacatttata ttatcttcag aagagttgaa aaatattcaa ggtgggagtg ccgtgggagt
    23281 tttaggcact actttttctg gggcaacagc aggagtaaaa ctttgttcag ccggaggacc
    23341 ttatgcaata gctgcttgtg gtgtaggtgg agctttatta ggtgctggat tcagcatgtg
    23401 gaccggtgcg taaacaatta aaaaatatca aacctcaaga ttggtttgtg tcgattacga
    23461 acataggtat gttctttatc ttttcaaaag tttggaatgg acagaacttt ttactaaagc
    23521 ttcttgtgct agtttttata attatattaa atttttatct tttattaaag tcagataaaa
    23581 attctttgga aatgtaatgg tagagatatt tttacaaaag gacttgcagc gctgtagttg
    23641 gtgctgcagg aggacctatt tcggccgtta caggaggtat tggtaatgta cttgtcggat
    23701 gtgcattaaa gcctgttcct gcaagttaaa tttataaaaa caggaaagta attatctcaa
    23761 atcctatcaa gtacaatcat aggattgcac ttgacaggac aaatttttta actaaaaaat
    23821 gaaaaacact ttctaaaaat tagtagaatt tgccaaatct ttcgcatctt ccaccataca
    23881 tttaatttta attccccttt atggtactta ataaaaacaa gaaaagaact attcatttgg
    23941 cccttttttg gttccagacc atagaaatat tcctccagta actaatattg ctgttatgca
    24001 cgtaactaat aacagctata aaaattggta caactttaat aggattcaga aaaatttgca
    24061 ctagtgaagg aatctatttt attagaaaaa tgcaatctcc ctaaagaaaa aaccactaaa
    24121 cagtgctttc tttcaacttc tccttaattt tttgtgcttt ccctaaagta aagtttctgg
    24181 ccaactctga atcaaaatat agtgaacgtt ttttaaaatc tatttcttta acgttaaaga
    24241 tatttactaa acacgctcga ctaattcttg ttaacatagg atatttcttt tcaagctcac
    24301 taattcttcc ataaaattcg tactgcccat ctttagtaca caatgataat ctatgtggta
    24361 attttgaagc ttctacaaaa taaatgtcat ttatatcaaa agtaaatgtt tgagaaccta
    24421 tggaaaaaac aaatgcttga ttcgtaacct gttttgttgc atctatcctt tcttgcgcta
    24481 ataacaataa ctcaacaatc tcgtttcgat actcatctag tgtttgatct tttgttacaa
    24541 atcctaacgt ttcaactctt ctttttattg ttacgggtaa catctcatca tgagtagttg
    24601 taaaaatgat ttttgcttga acatcatatt ttctgattac ttctgctaat tctataccgt
    24661 taacttcatg atttaaatca atatctaaga aatatattcc ttgctttggt cggaattgtt
    24721 tgaggtattt tttaacttct aacggacttt gtgttttcaa tactatctta aatactttat
    24781 cgtgaaaaag aatgaaatta tcaataattc gttctaactg atttaattgt ataatttgat
    24841 cttcacataa tattattgga taactcataa atcctccttc cctcaaaaca acaaaataac
    24901 ttgagtagta aattgatacg gatcgttttg atagttcaca aacatattgg gattcttttt
    24961 attgatttcc tctatactag ataaacctaa cccttgatgt cccttttttg ttgttattcc
    25021 ttttttcatt agagtatcta tagagagtag tatctctcta caactattcg atatcgaaat
    25081 ttctatctgt ttatcatctc ggtaaatagc tagagacagt tctctatctt gactctctat
    25141 agatgcttca attgcattat cgagaataat tccaagcacc ctaatacagt caaatattgg
    25201 aacaggaact tcaataatag tacttggaca ttcgaattta caacaaatct tattgttatt
    25261 tgcttggtat agcttcgcta tcaacaaact tttcaaatat tcatttttta tattttttaa
    25321 ataacgatat tcaaacttat tggttagata agaattagaa tagatctcta attcttttat
    25381 ttctttttcc aatatcgaat catggttttg attagcgatt tcttttaaac tcagcaataa
    25441 atttttatag tcatgtctaa atctggcaaa tttttcttga tctttttcca gtgaatcagt
    25501 atattttttt agataaacca attcttttct tttcaattgc tgttcatatt gttctcgttg
    25561 cctaatagtt attctaataa ataaaaatag aacaacaaac atttgaatga ttaaaaatat
    25621 taagacacca aacacaaaat gatcaaatac ttgatattga tgagcaacat aagaaacaaa
    25681 taaaccaatt gtaagtaaat aaatcatgaa tatagcagta agtttagaac tggactgttc
    25741 aacaatccta tttatagcta attttctata taaccaaata aacatcagca aaaacaaaga
    25801 atcaagcatt atctggaata ctacataacc ataccctttt atcccttcaa atgaaaacat
    25861 atacgtcata accatggaag taataataga tctgaaatca tcaataagca tacataatag
    25921 taatttattt aaaagcacta tattagtttt cttcttttcc tttaatataa aataacttcc
    25981 gattataggt atagctgcgg aaacatcagt aaataattca acaaaaaaag tgagtggtat
    26041 caataaacat acaaaaagat attttctttt gttaagatca gtattagtca tataaaataa
    26101 gagaaaaaag aaaaagtttc ccacgaacat tcttaataga taagcttttg tatcgatagt
    26161 aatcaagaga tcccctcctg attttaggat atcataaaaa aaattttagt tacacttttt
    26221 aaataatgaa aaaacacatt ccactaaatt tgatgccggt tttccttgag gttttgttcc
    26281 agcacctcct ttaatttttt taagctcttg atctgaacat tgtttagcat ttagtctatt
    26341 tttttcttcc attacgattc cccctacaaa ctataaatac ttttatgtca ctgattcaat
    26401 atacctattt ttctaatcaa aaaattttga gacatattta atattataag tatcattaaa
    26461 attgagattt atctccataa tctgctcgat ataaaccatt tattccagca gttcttccaa
    26521 tttcactcaa atttcttatt aattcctctt gatattcagt gaatcttatt ttattagtat
    26581 aagcagtaaa atatagataa ttcaccaatc tatttattaa aggaccggga tctaattcat
    26641 atttttgttt ttctaatttt aaatatacct tttgtaaaac ctcaagaata tcagaatatt
    26701 taggatcttt acttatagat atatcattat ataattcatg aacaatttgc ttagcatttt
    26761 ttttcatttt agcacttccc tggaattgct ccacctaaaa aaccacctat agacattcct
    26821 gcaatacaag tagttgcctt ggcccaatcg accgtacatt tatttttagt gcaatacact
    26881 ccatttccat aatattttcc actatgagtg gtacccccat agataagttg tgtctcttta
    26941 atagacaaaa tttttaaatg tttcataatc taatcacccc taaattaatg tttttagact
    27001 attattctaa cattcattaa atttcaaaaa acaattcctt cttgaaacgt agcaaaataa
    27061 ttcctgaata agaatttaat cacttttaag tatataaact atgtatattc tttaatattt
    27121 tttgagaatg cacttcgtac ataataaact ttaatcacat tagtctttca tacctacgat
    27181 cgatctcttt gactcgcacc cataaattca taatagtttg tcggcgtctt tggacgtttc
    27241 gaagatgagc tacgcattct ctttttttat agacaaaaaa gatagagacg aacatggagc
    27301 ttccaaattt ggatatttga tccaattttg gaagctccat ttatttcgtg tctgtctttt
    27361 tattttttcc agctttcgac taattcttgg ttctctttga tccagtcttt tgctgcttgc
    27421 tgtggatctt ttccattgtt gatttccaac ataacttttt ccatatcttt ttctgaccaa
    27481 tggaaattgt ctaatacttt gtaagcttct ggctggtctt ctttcaatcc ttttcgaacc
    27541 atcgtatgga tctgctcttc gcttcccatt ccgttttcag gatcttctaa gtatttcaga
    27601 tcatatttgg caaacatcca atgaggtgtc catcctgtaa ctacaattgg ttcgtgtttt
    27661 ttgatcgctt gacctagtgc aacggtcatt gctcctgaag aagatgtctc tactttccag
    27721 tctttcaagt tgtcatattt ttggatcgtg ttttcagcag ccgtaactac accagctcct
    27781 ggctcgattc ctatgatttt cttacctgcc tgatcggtta actcatcgat cgaatttaca
    27841 tccatgtagg ctggcactgc taaaccaact tttgctcctg tcaaattggc acctaggtct
    27901 tcgacttgat ctttatattg tgcatattgt gaaccatgag ttttaggtag ccaagctgaa
    27961 accatggcat ctgactcgcc atttgaaaca gatttccaca tgattgaatt atctaatggc
    28021 gtcatcgtta catcatagcc catttgtttc aatacttcac cgactacatt tgttgaagca
    28081 acttctgtat cccattcaac ataagataag ttgatacgtt ttgtttcttt ggcagatgaa
    28141 aaactcccta taccaatcgc acctaagatc actactacta cagcaccaat gatcagtcct
    28201 tgacgttttt tacttttctg tgttgttgct cctgcagctt ttttattgtt tggctggttc
    28261 aaatgctgtg taaaacggtc aatgatgatt gctaagataa ctaatgcaac cccgttgaca
    28321 aatccattcc cgacttgtgc tctttgcaat gctgataata cgccgcgtcc taagccgggt
    28381 gcaccgatca tagaggcaat aacgaccatt gaaagtgaca gcattgttgt ttgattgatc
    28441 cctgccatga tcgtactttt agctaaaggc aattctagtt tgaatagttt ttgacgactt
    28501 gtgctcccga atgaatcaga agcttctact aattctttag gaacttgtcg aatccctaaa
    28561 ttcgtaaaac gaacggttgg tggcagagca aagatgactg aggcaaatac ccctggaacc
    28621 atcccgatac cgaaaaatgc aactgctgga atcaaataaa cgaatcctgg cattgtctgc
    28681 ataaagtcaa ggatcggttt gataatactt tgggctttac tgctttttgc catcaagatc
    28741 cctaatggta ccccaatgat gatcgagatc acactagata aaagaacaag tgtgacagta
    28801 ttcatcaaat cattccatag accttggtta taaataaacc atagaccgat taatgtgaac
    28861 aacgtaagtc ctggacgttt cttagaaata aagaaagctg ctaccgtcaa taacaagata
    28921 aacaaaggag ctggaatcac taacagtaaa tttgtaatcc ccgacatcac tgcctgtcca
    28981 atcgtttgta aaaatgaaaa taggccagaa aaagttgtcg taaaccattc tgtgatattt
    29041 tctacccagt ttgccactgg taattgatag ttcaatagat tattcatgtt cactcacctc
    29101 gcttgtttct gctaatgctt caatcacact acctcggatg acgacacctt ttaattttcc
    29161 atcctctaca actgcaagtg gagtcggtga gtcataaata atattgaaaa tatcacttac
    29221 caccatgtct ttcccaatca ccgtcacatt ttcatctagg aagtcgatca atggacgttt
    29281 ttgcttccta gcttctaacg cttgatctgc agtgatgatc cctttcaggt gtctctttct
    29341 gtcaacagcc atcagcatgc tgacttcttc attacgcatc cttgtcaatg caacgtttgg
    29401 tccatcagat tcgatattcg tcgtcaatgc aggtaccatg atgttttgag ccgtaagcac
    29461 tttggaacga tcgacttctt ccacaaattc acgtacataa tcatttgctg gattggtcaa
    29521 tatttcttca cctgttccga tctgcatgat ttcgccgtct ttcatcaagg cgattctgtc
    29581 cccaatccgt aaagcttcat tcaaatcatg ggtaatgaaa ataatcgttt tttgaacatt
    29641 ggcttgcagg tccagtaatt cgtcttgcat ttctcgccgg atcagcggat caagagccga
    29701 gaacgcttcg tccatcagca agatctcagg atcatttgct aaagctctgg ctaggccaac
    29761 ccgttgttgc atcccaccag ataactgact aggatattgg tctttaaatg aaagcaaact
    29821 agaattttct aatgcttttt ctgctttttc ttgtcgttct tctttaggta caccacggac
    29881 ttctaacccg tattctgtat tttccaagat cgttcgatgt ggaaacagac cgaagttttg
    29941 gaaaaccata ttgattttat gacgacggac ttctcgcaat tcttctttgc ttaacttcga
    30001 tacatcttct ccatcaatat agatactgcc cgaagttggc tcaatcagac gattcaatag
    30061 acggatcaat gtggatttcc cactcccgga aagccccatg atcacgaaga tttctccttc
    30121 ttttacgtca aaattcacgt catacacacc aaccgttgca ccggttttct ttaaaatctc
    30181 cgttttcggc tggtgatcat tcatcatatc taaagcggct tgtgtctttt taccaaatat
    30241 ctttgttaaa tgttttactt ctacttttac caaacaaatt ctcctttttt atgtaaattc
    30301 atttttcttt ctgaaaaaag tgaccacact atgttaacat catgtagtca ctttgtcaaa
    30361 aatgaaagaa atttgcttgt ccaaactgaa aaaaacttgg ctaaatcttt gtaaataaaa
    30421 ggtttagcca agttaatatt tttttaatat ttcacacaat cattctgcaa gctgatgctc
    30481 cgggtaaaaa aagtttgtca tacgctgtag tgtatattca tttccaataa aatgaacgat
    30541 gtcccctgcc gtaaatttag cataggggcc aggtgaaaca agcagttctt cattatgaga
    30601 aattgcgata actgtggcgc cagtcgcctg ccagatattc aactcgctaa ttgaacggtc
    30661 cagatgcatt gctttttctg ataaagataa ttcaaacgga agtaaaggat tcatcttatg
    30721 tattcgcttc gtttgattga ccagctgatc caataattga gaaaaatgat tcagctcatc
    30781 ttgttgcttc tccacacttg ccatgatttc tgcttttgta tcttggatgg attgtacatc
    30841 ttgatattgc aataggaaat cttttgcttt ttccttcgaa gcaacaaatg ccccacttcc
    30901 atgccgtact tccatgattc caagatctac cagcacattg atagccttgc gggtagtctc
    30961 aggagaagca ttgtacgtac tggcaagtgt tgagcgggca tggatcttct cacccacttt
    31021 atatcggttt tcaacgatac gttcggctaa atcaacagca atctgctggt agcgcgggcg
    31081 gacgattttt ttctttccag acattttctt tcctctttct ttttttactt tccctcatct
    31141 tactatgctc tagcaaaata agcaaaaaag gggagaatag cctcttgcaa cgactgatca
    31201 tcgcttgatc tgttcctttt ttactgggca agctgtttga cagtcatcac aattcttacc
    31261 ggattttaaa cgagtataga caattgcgcc tgcacttcca aagattaaca cacttaataa
    31321 gaatgtcgcc atcctattcc tctccttcta atgacaatat agtataaaac tggttcttgg
    31381 attttggctg acggaacaaa acataaacga gtcctatcat cagtaaaata gcaagatacg
    31441 tccctaacgc tacctgtcca ccctccaata agtgtcccaa ctgataaatc acaaaactaa
    31501 ctaaataagc taaaccgcat tgatagccta tggcgatcca agtccatttc ttggcattca
    31561 tttcccgatg gatagcccca attgccgcaa aacaaggcgc acaaagtaaa ttgaaggcta
    31621 acaatgaata agctgctgcg ggagtatatg tgtgttgtag cacttgccag atttctctgc
    31681 cattttcgga tacttccgca tgaccaaaca aaataccaaa tgtgccaata atattttctt
    31741 tagcgaccaa tcctgtaatc gctgcgactg ttccttgcca gtttccccaa ccaagcggct
    31801 gaaaaacaac tgccaggatt cgcccgaaaa aggctaagat actttggtct tcagaaacag
    31861 gttgagcata aaaattataa gaagagctga accagatcac gatactagaa acaaagataa
    31921 tcgttcctgc ttttttaaca aaagacttgc tgcggtcata ggtttgtctt aggaccgctg
    31981 accattgagg aaaatgatat gcgggcaatt ccatgataaa aggagctgta tctccagcaa
    32041 acaaacgtgt ttttttcaag ctgattccag agaaaataat tgcagtcata ccaagaaaat
    32101 aggcagaagg agctacccaa gattgatgag gaaaaaaggc ccctgctact aaggcaataa
    32161 tcggcaactt tgcagaacaa ggcatgaagg ttgtgaccat gatcgtcaaa cgacgatctt
    32221 gctcgttttc aatcgtgcga ctggccatga cgcctggcac tccacatcct gtagcaatca
    32281 gcataggaat gaaggatttt cctgaaagac cgaatttcct gaaaagtcga tccatgacaa
    32341 aagcgatcct cgccatatat ccgcagtctt ccagaaagct caagcataaa aacaatataa
    32401 tcaattgcgg tagaaatcct aacactgctc ccacgcctgc gatgatgccg ttcaagatca
    32461 gctcctgcat ccatcccgct acttgccatt cagctaacaa tctgccaaca tgttctggta
    32521 accatgtacc aaacaatgtg tcattgatcc aatctgtccc cattgttcct actgtttgaa
    32581 tagccagata atagatcagc cacatcacaa atgcgaagat cggcaaggct aaccaacgat
    32641 tcgttgcaat ctgatcgatt ttgtcactca tcgataattg gaaagaatca tttgacacaa
    32701 cacagagtgt acatagttgg gtaataaatt cataacgttc attgaccaga atcgtctcac
    32761 tatcgtcgtc caatagtttc tctgttaact ggatcagttg ctcgatctct ttttgctgag
    32821 aaactgctat ctcgtaatcc tctcttacct tctgatctcg ttcaaaatat ttcattgcat
    32881 accagcgttt cttgccgctt tgttctccta gtacatcttc aatttcatgt aaagctgctt
    32941 ctaaacgtgg atcatatgta cagggaacag gaatagaagg ccgctttttt gcagactcct
    33001 gaatcttttc gattagtttt tcgattcctt gctttttcaa tacgctgatt ggtaaaactg
    33061 gtacaccaag accataggaa agcttttcta gattgatttt ttttccttct tttttgatga
    33121 catccatcat attcagacca atgatcatag gaatgtctaa atccaataac tgcgtagtta
    33181 aataaagatt tcgttctaaa ttggtagcat caataatgtt gatgatcagt tcaggctgtt
    33241 cctgaaccaa ataatctctt gcaatcactt cttctggtgt ataaggagac aaagaataga
    33301 tgccgggtag gtcctgaata tgaatgttcg gatttttttt caacgatcct tctttttttt
    33361 ctactgttac gcccggccaa ttccctacat attgattcga accagtcaag agattgaaca
    33421 agctagtttt cccgctgttt ggatttccga ctaacgcgat attcattctt tttcctccct
    33481 ttgtatcaag attagttcgg cctctgactt tcgtaacgtc agttcatacc ctcgcactgt
    33541 cagttcgatt ggatctccca gaggggctag ttttcggata actattttta catttcgcgt
    33601 caatcccata tccattaacc gtcgcttcac tgcgccttgt ccgtggattg ctacgacgat
    33661 cccagtctct cctgctttta attgatcaag agaagtccag ctttcagagg aagcagtctt
    33721 tttattgaca aagatttgac ctagtatttc ccgacttact gccactcgat gactttttac
    33781 cagtaaaatg gcattgttgc ctgataaatt gacgacgacg attttacttc cggcaacgat
    33841 cccgatattt tgcaaatggc gttttgtttt ttcttctgtt tcgattcgtt cgacttgata
    33901 aacgaaattc tgctttgcct gcgctaaggt ctgcatattg attcctctct tgtgaaataa
    33961 atcatgtatt aaaatcattc attatatgtt ctaattttct tacgtgttac gcaaataaat
    34021 gagaatcatt ctcacataga atactctcgg taattgagaa tgtcaattat acttgtgata
    34081 ttatgagcga atctttagta ttgatagata aaaatgagaa aaaaatattt cttcatagtt
    34141 ttccacaatt ttttctctat taaatttgct attttcccac acgatacttg ttttcagctc
    34201 ttttttcaaa aacacaaaac ttagtattcc acagtttcca caacacaata tgtagtgttt
    34261 gttgtttgac ttttttacta agagttttat actatctatt atagataata agaatgtgtt
    34321 agaggaggca ctcaccatga acgtaaaaat cttttctaaa aataattgta tgcaatgtaa
    34381 gatggtcaaa cggtttttaa cagaaaatca aattgctttt gaagaagtca atatcgatga
    34441 acagcctgaa gcagtagact ggttgaaagc acaaggattc caaagtgttc ctgtcattac
    34501 ttctgatgca gaaacagtta tcggattccg tcctgatcag ttgcgtaaac ttgccagcta
    34561 acatgactaa aagaatcaaa acgtgataca tggatttgaa gggcttgaga cagcaccagt
    34621 ttttgtcgca aacttccaaa tcctttttta aagcttaaaa acaatgcaat gatttgcaat
    34681 caagcatcat caaatacgaa atgaaggttg ggcgtacgta tgagtcttaa aaatttacaa
    34741 gatgtcagtt atttcaagct gaataatgaa atcaatcggc cagtcaatgg tcaaatccct
    34801 ttgaataagg ataaagaagc tttagttgcc ttttttgagg aaaatgtcaa accaaatacg
    34861 atgacttttc tatctattat ggaaaaaatc aattacttga tcgatcaaga atatatagaa
    34921 gcagaattca ttcaattgta ttcaccatca tttattgaaa agctatatca atttttggac
    34981 gagcaacatt tttctttcaa atcttttatg gctgcttaca aattttattc tcaatatgcg
    35041 ttgaaaacca acgacggttc cgcttatctg gaaacttatg aagatcgagt agccttcaat
    35101 gcgctttact ttgcgaacgg ggatgaagaa ttagcacttg ctcttgcaga cgaaatgatc
    35161 catcaacgat atcagccagc tacaccttct ttcttgaatg ctggacgtaa acgtcggggc
    35221 gaattggtgt cttgtttctt gactcaagtc actgacgata tgaactctat cgggcgctca
    35281 atcaactctg ctttgcaatt gtcccgtatt ggtggcggcg tagggattac tttatcaaat
    35341 cttcgtgaag ccggcgctcc tatcaaagga tatgagggtg ctgcaagtgg tgtcgttcca
    35401 gtcatgaaat tattcgaaga tagtttcagc tattctaatc aattaggtca gcgtcaagga
    35461 gctggcgtgg tttatttgaa cgttttccat ccagacatta ttgctttcct ttcagcaaaa
    35521 aaagaaaatg cagatgaaaa gattcgcgta aaaactcttt ctcttggcgt gatcgtacct
    35581 gataaatttt atgaattgac acgtaaaaac gaagatatgt atttatttag tccatatagt
    35641 gtcgaaaaag aatatggtgt cccattctct tatgtaaata ttacagctga gtatgatcat
    35701 ctagttgcaa atccaaacat tcgcaaacaa aaaatcaaag cgcgtgatct tgaaaatgaa
    35761 atctcaaaat tacaacaaga atctggctat ccttacgtaa tcaatattga tacagcgaac
    35821 cgcgagaatc caatcgatgg caagatcatc atgagtaatt tatgttcaga gattttacaa
    35881 gttcaaacgc cttctgtgat taacggcaaa caggaatatg aagtattagg aacggatatc
    35941 tcatgtaact taggctcgac aaatatcgtg aatttgatgc aaagccctaa ctttggtcaa
    36001 tctgtgcgta cgatgactcg tgcattgaca tttattacag atgcatctga tatcgatgtc
    36061 gtaccaccga tccaaaacgg aaataagctg aatcatacga ttggattagg tgccatgggc
    36121 ttacacactt atcttgcaaa agaacaaatg gaatatggct cagaggattc attagacttt
    36181 acaaatattt acttcatgct gttgaattat tggacacttg ttgaaagcaa caatatcgct
    36241 cgtgaaagaa aacaagtttt ccataatttt gaaaaatcag cttatgctga cggcacttat
    36301 ttcgaaaaat acgtaactgg agaattccag ccaaaatcag aaaaagtcaa agcattgttt
    36361 gaaggcattt ttatcccaac tgctgaagat tggaatgctt tgaaacaagc agtaatgaaa
    36421 gatggactgt atcatcaaaa tcgcttggca gtagcaccaa atggctcaat ttcatatatc
    36481 aacgatacga gtgcaagtat tcatcctatc actcgcatga tcgaagaacg ccaagagaag
    36541 aaaatcggta aaatttatta cccagcgcct tatctatcca atgagacgat tccgtattac
    36601 acttctgctt atgatatgga catgcgtaaa gtaatcgatg tttatgcaat ggcacaacaa
    36661 cacgtcgatc aaggcatgag tttgacttta ttcatgcgtt ctgaaatccc agaaggcatt
    36721 tatgaatgga agacaacaac gaagcaaacg acacgtgatt tgaatatttt aagacattac
    36781 gctttccaca aaggaatcaa atccatttat tacgtacgta cctttactga tgatgcagaa
    36841 gaaatcggaa gcaatcaatg tgaaagctgc gtgatctaat atcatagata gattcgctta
    36901 ttcatatttt aattaaagga gcattctcat gtcagaaaca tattatgaag caataaactg
    36961 gaatgaaatt gaagatatca ttgataaatc cacttgggag aagctgacag agcaattttg
    37021 gctagataca cgtatcccac tttcaaatga cctagatgat tggcgtacat tgtctcaatt
    37081 ggaaaaagat acagtaggac atgtatttgg cggtttgaca ctgctagata ctgtccaatc
    37141 tgaaagtggc atggatcagt tgcgtaaaga tgtccgcact ccccatgaag aagccgtttt
    37201 gaacaatatt caattcatgg aatccgttca tgccaaaagt tattcttcta tttttagtac
    37261 actgaatacg aaaaaagaaa tcgaagaaat ttttgactgg acaaatacga atccttatct
    37321 gcaaaaaaaa gctgagcgta tcaatgaaat ctacaaacac ggtacaccgc tagaaaagaa
    37381 aattgccagc gtctttttag aaacattctt gttctattca ggtttttata ctcctcttta
    37441 ttatttagga aacaacaaat tggctaacgt agcagagatc atcaaactaa tcatccgaga
    37501 tgaatctgta catggaacct acatcggtta caaattccaa ttaggattca acgagttacc
    37561 tgaagcagaa caagaaacgt taaaagactg gatgtacaat cttctttatg agctttacga
    37621 aaacgaagaa cgttatacag aagaattata cgatccaatc ggctggacag aagaagtcaa
    37681 gacattccta cgctataatg ccaacaaagc attgatgaac ctagggatgg atccactctt
    37741 tccagatact gccaatgatg tcaacccaat cgtaatgaac gggatttcaa caggcacaag
    37801 caaccatgac ttcttctcac aagtcggaaa cggctatttg ttaggtcatg tagaagcgat
    37861 gaaagacgac gattatttga ttggattgga ataaggattt attttaattc aaaaattttt
    37921 cgaaaaataa aaagagttta gcgtacagat attttaaatg tacactgaac tcttttttat
    37981 gcttattcac ttgtttcttc cagcactttt ggactgtcta acttttccga aacatgttcc
    38041 attcttctca ataaatagat tttccggata tttaatatta tcgtcttaat cactgcatag
    38101 acaggtatgc aaatcagcat gcctaatatt cctgcgacac ttcctgctcc aatcaatacc
    38161 aagatgatcg tcaaaggatg gatattcatt gatttcccaa ataacaacgg cttgatcaaa
    38221 tttccatcta attgttggat cactaaaatc gacaacgcca tatacaaagc ttgaaatgga
    38281 gagataaaca aaccgacaat gattgctggt accgctccaa taaaagggcc gacatacggg
    38341 ataatattcg tgattccgca aatgaccgca agcaacaagc tataaggctg atcgaatatt
    38401 ctcattgcca aaaagctcat tacaccaata atcaaagcat ctaagatcgt actgctgata
    38461 taggcagata atgtttcgtt taattctttt accgtttggc gtaactcttt acgaatcgct
    38521 ttcggaaaga agtgagacaa cgcatcaata aatttgtgcc catccttgaa cataaatagc
    38581 aaaataaatg gaacggtaaa taacaaaata aaaaattgca tcataaatga aatgattttt
    38641 gagacacttg cagataaact gactaatatg atattgaaca aattactaag agataaattt
    38701 gctgatgcta attgttcttc caaattgaaa tttttaaact cttcccgctg cgccaattca
    38761 tttaaccatt tgcttgtttc atctgcatac attggcaagc tctgggtcaa ttgaattgtc
    38821 tgttccatta actgaggcac tacattcatg acagttaaaa caactaaaat gattacgacc
    38881 aaaaacgtca ataaaaaacc gaacactctc gggatccgat gcttttcgag aaaaacaacg
    38941 attggatcga acatataata gataaaacca gcaacaagca atggtaaaaa aactgccgaa
    39001 agcattttca atattggact aaaaatattc ggcatttgta aaataaagta cacgccgatg
    39061 atcgtgagta ctagctccac tgtccagaaa aataattttg attgtttaaa acgtgattgc
    39121 atacatctcc tccaaggtcc tttgatactt ctattctatc atttaacaaa gagttacgcg
    39181 ctgagatttt catttgctta aaaaaaacaa aaaaaataaa catattttaa tgagtagacg
    39241 agtacttaaa caaaaacacg gtttggagta gtgacaaaaa ttatactgca gtcccttttt
    39301 ccaaataaac ggtgttcaaa agcttgcatc ttcgtaataa gcccagccaa acgaaaaata
    39361 taaggaacta tttttatttg gctgggctta ttacttgatg taggacatct attttacaac
    39421 ctcagatcat ttattgcgca atactgacta aatgcaactt tgtgatattt ttgtgtacgg
    39481 acaactcctc gatcacatcg gcataagtga gcccttttgg aagatcaatc gtatagatat
    39541 ttttgtacac tcggtagtca tcaacgaatt ttacgtcaaa attcacatct tcgattacga
    39601 tatttttctc ttcaaaataa tcggtaagaa aatctttggt ttcttttcta tggataaatt
    39661 ggatttctaa ctttttagtc gtaggtacat ggatcacgcg tttcactact gtgagtgcaa
    39721 tagcaatccc gataaaactg atgatcgcaa tggaatagta acccatgccg atggcgatcc
    39781 ctaatccagc aattgcccat aaagaagcgg cagtcgtaag tcccgtcacc gattgtttcg
    39841 tgacgatgat cgttcccgcg cctaagaaac cgacaccact caccacttga gcaatcagcc
    39901 ttgcttcatc tgaacgaatc gttccagcaa gctgtggata ctctaaagca tctcttaatg
    39961 caccactagc gatctcaact tggatcaatg caatgataca tgctcccata caaactaaaa
    40021 tatgcgtacg catacctgct ggacgatttt tatattgtcg ttcaaaaccg attgctccgc
    40081 caaatagcat agcgatccct aaacgtaaaa taatctctgg aattgtcaat accatatcat
    40141 tcatctaaac accctccagt ccaatatatc atgtctgaaa ggagcagaca aaaaaagtgc
    40201 aatttttacc tcttacgaaa aaaggactgt gacaaaattt ttgtcatagt cctttttatg
    40261 aataaacggt gttcaatcag ctgaccctcg gtattactca acaaacggca aaatatgggg
    40321 agctattttt cctttgtctt ctctaatact caggtcaaaa cgctgatttc acaacctctt
    40381 tagcctacta ttgaaaatta caacttactt gctgcttctt catcgatgat cacaacaaca
    40441 ttgtcgtgat tttgcaatac acttgctggc aaatgattcg ttacaggtcc ttcaatcatc
    40501 cctttgattg catcagcttt gctttcgcca aatgccatca agacgatttc tttacttttc
    40561 ataattgaac cgattcccat tgaaacagct tttgttggta catcttctgc tttatcgaag
    40621 aatcgtttgt ttgcattgat tgttgattct gttaagtcaa ctactgaagt cgttccgtca
    40681 aatgaagcac caggttcatt gaaaccgata tgtccatttt gcccgattcc taagatttgg
    40741 atatcgattg gatgtgcatc gatgatttga tcatactttt tgcatgctgc ttccagatct
    40801 tctgcttttc catctggtac aaacgtttct ttgaatggtt ttttatcaaa taattggaca
    40861 tccatgaaat gacggtagct ttgttcatct gaaccgccta gtccaacgta ctcatctaag
    40921 tttacagaag tcatttcact gaaatccaaa tcgcttgaag tcatttcttt atataaagta
    40981 actggtgtgc ttcctgttgc caatcctaat acttttgcac ctttgttcat accttctttg
    41041 atgatttcaa atgctttttt tccgccttct tcagcgtttt ttacacgaat gatttccata
    41101 gtgattgctc ctttatattt tggtatagcc caattatagc attctttttt tctttttaca
    41161 ataattagta tagaccaaat tgcatttttt tcattttctt gacaataaat aggaatccgt
    41221 gttaggtttc ataagatggt tcaatcaaac aatccatgtt gcatgaaaac caagcaagtg
    41281 attcactgtg gacgctttat gtctgcagtt tttttatgaa aaaggagtta ttatgagtat
    41341 actgacatta aacaacatta cccagcaatt tggcgacaaa atcctctatg aaggtgtcga
    41401 tctgcaatta aatgctggcg aacatcttgg gctgattgga caaaacggag ccggtaaaag
    41461 tacattgatt aaaatcatca ctggtgagat cttaccagat gacggacaga tccattggca
    41521 aaaaaatatt cacaaaggtt acctcgatca atatgtggaa gtcgatgaaa cattaacaat
    41581 cgaagaattt ctaaaaactg cgtacgcaaa agaatacgaa aaggaagaaa aaattgcttt
    41641 tctttaccaa acatatagcg agaccttcga tgattccttg ctggaaaaag ctgggaaact
    41701 ccaaacggaa ttagaccaag gagtgtttta tcaaatcgac accttagttg ctgagatgag
    41761 cagcgggttg gggatcgatg tcttaggttt agattcactg ttgaaaaatc taagtggtgg
    41821 acaacgttca aaggtgattt tagcaaaact acttttagaa aacccagatg ttctgatctt
    41881 agatgaacca accaatcact tagatgatca gcatatccaa tggctgacgc agtttcttca
    41941 ggattttgac ggtgcgtata tcgtgatttc ccatgaccaa gaatttcttg atcagatcac
    42001 cacccatatc gcagacatcg agtttggaaa gattactaag tatactggcc atctaaaaca
    42061 agcattgaaa caaaaggaac aaaatcgcga aagctatttg cgccaatatc atgctcaaca
    42121 aaaacaaatc gaaaagaccg aagcttatat tcgaaaatac aaagccggtt cccgatcaac
    42181 gatggcaaaa agccgacaaa aacaattaga taaaatcgaa cggctgacgc ctccagcttc
    42241 agcaagtaaa gctgtctttg attttccatt ctcgcctatt gtcacgacat tagcagtgga
    42301 aacaactgat ttagtcatcg gatatgatcg accgctactt gcacccatca atttgaccat
    42361 ccgctttggt gaaaatattg ccattcgagg cttcaacggg attggaaaat ctactttatt
    42421 aaaaacactg atcggtgaga ttgaaaaaat aagtggcgat ttccacttcc cagataacac
    42481 aaaaatcaat tacttttcac aagatctgta ctgggaaaat ccattagaaa caccactaca
    42541 atatttaagc agtcaatttc caaaagcgac tatcaaagaa ttacgtcgcc aactagctaa
    42601 agcagggtta gttaatcaat tagcaagtga acctctcact acattaagtg gtggggaaca
    42661 aactaaggtg aaacttgcac agttgacaat gaaccaaggt aatttactga ttctcgatga
    42721 accgacgaac catatcgatc aagaaacgaa agaaagctta caagacggga tacagaaatt
    42781 tccaggaaca gtaatcatcg tctctcatga acaagaattt tatcaagatc ttgtgcaacg
    42841 agtcattgag attgaaccaa aataaacgtt tgattgaata gttgaaaaaa aggagctgtg
    42901 acaaaagttt tgtcacaact ccttcttctg aataaatggt ggccaattag ctgaccttcg
    42961 gtattagctc aaaaaacggc aaaatatgag gagctatttt tatttggcta ttcttattac
    43021 ttgatgcaga acagcttttc acaacctctt ttcgtctatt ttttattttc gagatttctc
    43081 tttcctttaa aaacaactgt ttgaaagaag aacgcacatc cttgaaaaac aagcccgcta
    43141 accgttacac ctaaccagcc atactgctgc cacattgtcg tccctagcaa tgaaccagcc
    43201 gcacctccaa taaagtacat aaacataaat acagtattat ttcggttgct tgctgcttca
    43261 cctagccctt gtactcgtgt ttgattggcg acttgaccaa attgattccc gacgtctaat
    43321 aaaataatag ctaaaagcaa aatggccaaa tggcttccgc caaccaataa agcaataaag
    43381 cttattgttt gcataacaag tcccatatac acgatttttc tttcagaata agaatctgcc
    43441 agtcgtccca cgataggtgc agcaactgct ccagacaaac caaaaatcgc taatatccct
    43501 gcttcgaatg ttccccaatt gtaggcggga ctgctgatat aaaaaatcaa cgtagaccaa
    43561 aagatcgaaa atgttccaaa catgaaaaat ccactgactg ccgcttctct taatagacgt
    43621 tgagaagcaa ctaaatgcgg caaactttta agtgaatcaa ggtatgaaac agctgtacta
    43681 ctttgctgga cagaacgcgg cattttccag taaagcagaa gtgtaagaat tccgactgca
    43741 cataaagcaa ataaatagat catccgccaa gaaaaaagac tggcaacaat accagatacc
    43801 gtccgagaaa gcaaaatccc cacgagtaaa ccgctaagca atgtacccat gaccttccct
    43861 cttgcttttg gtcccgctag taccgcaccg taaggaatga tgatttgcgg cacgatcgaa
    43921 agtaatccta ttaaaaaaga agcgcatgca aataacgtaa acgaaggcgc aaaaaaagcg
    43981 gccaacagag agacggcaga taacgctgcc attcgaatga tcaattttgg acgatccacc
    44041 acgtccccta aaggaactaa aaacagtaac cctaacgcat agcctaattg ggtcaacatc
    44101 gtcaatatcc cgactgcact tgtgctaaca gataaactag ctgctacttt tgttccaatc
    44161 ggctggatgt aatacatatt ggcaacaaca acgccacaag tgattgataa tagtaaaata
    44221 agcgaagttg ttaaaggttt tttttcatta ctcttcattg atttccccca tttcctatct
    44281 tagtttacaa cttatggaaa agattggaaa tagaaaaata caactattcg ccacacttat
    44341 tatttgtgct tttgttttcc ttgttttttc ttgcgatctt tataaatgaa gacaactaac
    44401 cctactagca cagtaacact tgcaaccaac gtgactgcag ttttgttata tccgtgaaac
    44461 agagcttctc caccacacgc atacaacaca gacgaaggga tgactccaat cacgatcgac
    44521 aatatgattt gctgtagagg caatttcatc gcatctgcag caaaattgac catagaagat
    44581 ggaataaccg gtatcatata gcctaacatt acacctattt ttggatgttt catttgtcgg
    44641 attgcttgca cccaatgatt cgtttcggtt ttcttatcta aaaattttaa gcgatgcata
    44701 aggaaaattg cagtgatgtt accaagagca ttacccaaaa tgttgatgat cgcacctgtc
    44761 agtggtccaa agctcaaacc agcaatgact ccaactactg aagtaggaat acctggaaca
    44821 gcacacatca ccgctgtcaa tacgatcaag agtatcgatg caaaagtccc atgcgaacga
    44881 atatgtgcga gtagctctgc cttactcgct ctcggattta aaaataattg gatgtctgtc
    44941 tgatattcta aatacaaccg atagcccact accagtatca gaatgattcc caacgagatc
    45001 aatatcaatc ggattatttt ctttttcata tttcctcctg aaaaaaatct atattcatta
    45061 aataatagtt taacataaaa acaaaaaagc accgaaatcc aacataggat ctcagtgctt
    45121 ttaaatagta aaaattattt aacagttaca gatgcgccaa cttcttcaag tttagctttc
    45181 aattcttcag cttcttcttt agatacgcct tctttaactg gtgcaggagc gccatcaact
    45241 acagctttag cttctttcaa gcctaaacca gttgcttcac gaactgcttt gataacttta
    45301 actttttggt ctccagctgc agttaattct acagtgaatt ctgtttgttc ttcagcagca
    45361 gcagcgccac cagcagcagc tacagctaca ggagcagcag cagatacgcc aaattcttct
    45421 tcaatagctt taactaggtc gttaagttct aggatagttg cttcttttaa ctcagcaacg
    45481 atgttttcaa tgttcaatgc cattataatt tcctccgttt aattgttctt ttttatttaa
    45541 tgctttatgc ggcaattaag ctgcatctcc atcttctttg tctgccactg ctttgacagc
    45601 gtaagccacg ttgcggactg gcgcttgtag tacagatagc aacatagata gaagtccttc
    45661 gcgatttggc aattttgcca atgctgtgat ttcttccaat gaagagactt taccttcgat
    45721 gataccgcct ttgatttcta atgcttttgc ttctttagca aattcgtcca tgattttcgc
    45781 tggtgcgact acatcttcgt tactgaatgc aacagctgta ggtccagtga atacttcgtc
    45841 catgccttca aggccagctt ttttagctgc acgtgaaaga atagaatttt tgataacttt
    45901 catttctacg ttagcatcac gaagttgttt acgtaaacgt gttacttcat caactgttaa
    45961 tccgcggtag tcaacgacta ctactgatgc agctgctgta aatttttcgc ttacttcatc
    46021 aacgattgct gctttttttg cgataattgc ttcactcatt tttgagtttc acctcctgaa
    46081 ttttgatgga agagtttttt gcactgaaaa ggcaagtcac ttttatagtt actttgctca
    46141 accagtatta ttcatcacct attcacttcg ttcatagtga ttctactcgc tttgctcgaa
    46201 aggaaactcc tttcgctttg ctcaaccagt attgttcatc atctcttcac ttcgttcata
    46261 gtgattcaca ataaaaaact ctatgccacc gcagacatag agggactatt gaatatattc
    46321 aataaacgtc ctcggtagga aattaaggct tgatgccacc tactgtcttc ggtacaagtt
    46381 aattattaac ctcaactagc ttagcatgct aaactagttg agtcaagaat tatttttcag
    46441 ttggatcaat caacattcta atatttaatt agaaagaagc ttggtcaaca tggataccag
    46501 gtccaaatgt tgttgtgatc gttacatttt tcatgtattg acctttagct gctgatggtt
    46561 tagcttttag caatacatca tggattgctg caaagttttc aactaatttt tcatcgtcaa
    46621 atgaaacttt tccgattggt acatggatat ttccagcttt gtccacacgg taagttactt
    46681 taccagcttt cacttcgttt actgcttttg ttacgtccat tgtaactgta ccagttttag
    46741 ggtttggcat taagcctttt ggtcctaata ctcgtcctag acgacctaca gtagccatca
    46801 tatcaggtgt tgcaacaact acatcaaagt caaaccatcc gttttggatt ttttgaacca
    46861 tgtcgtcgtc acctacgaaa tctgcaccag ctgcttcagc ttcttttgct ttgtctcctt
    46921 tagcaaaaac taaaacagtt tgtgttttac cagttccgtt tggtaataca acagcaccac
    46981 ggatttgttg gtctgctttt ttagggtcta cgttcaaacg gtatgcaact tcaactgttg
    47041 catcaaattt tgcaatgttt gtttcttttg ctaaagctac tgcttcggca acaggataaa
    47101 ctttacttga atcaactttt tttaatgcat cttgcatttt tttgctcttt ttagccattt
    47161 tggttcctcc ttgattgtgg ttataacggt tgtacctccc acgtgttcta tatctttcaa
    47221 tatagagcca actgagaagc tgctgtctta gggtgtatag attattctac agtgatcccc
    47281 atgcttcgtg cagttccttc aaccatgcgc attgctgctt caacgttagc tgcgtttagg
    47341 tcttccattt ttaattcagc gatttctctt acttgatcgc tagaaacttt cgcaacttta
    47401 tttttgtttg gttcgcctga acctttttcg attttagctg cttttttcaa tagaacagca
    47461 gctggcggtg tttttgtaac gaatgtgaat gaacggtctt cgtatacaga aataacaacc
    47521 ggaatgatca aacctgcttg atcagctgta cgagcgttga attcttttgt gaatcccatg
    47581 atattgatac ccgcttgacc tagtgcagga cctactggtg gagctggagt tgctttacct
    47641 gcaggaattt gcaatttaac gattttttct actttctttg ccacgagaca tacctcctta
    47701 agtccgtgat gtggttaatt ggagatgcat atttctcctc ccacgtttta tctgatctga
    47761 aaaaatccag acaaataatg tgcaaaacaa tgcacactgg tacagtatag caaatttatt
    47821 tgttttttca agattttttt ctgctatttt tacattttgc ttgaataaac gctaaattta
    47881 ttctaaaaaa acaacaattt ttcgttctca tttcttcttt tatgttacac ttgcgctagc
    47941 atggttcaac agatgaaagc aaacaataac acggtttttt agacccttgt acaaagattc
    48001 ctacataaca aaatgaggtg gagaaaatga aagatttaag gtatagaagt gtctttgata
    48061 ttattggacc tgtgatgatt ggtccaagta gctctcacac tgccggagct gctagaatcg
    48121 ggaaaatcat acgtagtata tttggcgaac agcctgattc tgtagatatt tatctatatg
    48181 aatcatttgc aaaaacctat cgggggcacg gaacagatat tgcattagtt ggcggcctgt
    48241 tagggatgga acccgatgat gaacgtttag ctgattcttt gaaattggca tatgaagccg
    48301 gtatggaagt cttgtttgtt cctaagagtg aaaaagccga acatccaaac tctgtaaaga
    48361 tgcttctaaa aaaaggaaca cgaaagcttt cagtcactgg catttcgatc ggcggtggca
    48421 atatccagat ttctgaattg aatggattca aaatttcttt aagcatgggt actcctacct
    48481 tgatcgttgt ccaccaagat gttccaggta tgattgctaa agtgactagt atactttctg
    48541 agaccaatac gaatatcggc actatgacag tgacacggga atcaaaaggt gaaaatgcca
    48601 ttatgataat tgaagtagat gatcctcaag tagaagaaac ggtaaaaaga ctcaaacagc
    48661 tgccaaatat cgacagcgta aattatttcg attaaggagc taaaaaaatg tttttatcga
    48721 ttgaagaatt agtagatcaa gcaaaagctt atccaagtgt agcagaattg atgattgcag
    48781 ttgaaatgga aatgagtggc cgttcccgta gtcacatcat cgagatcatg gaaaaaaacc
    48841 tctcagtaat ggaacaatca atcaacgagg gaaaagaagg tgttacctcc gtcacaggaa
    48901 ttaccggtgg tgatgcaaaa aaattagatg attatttaaa tgaggcagac tttttaagtg
    48961 gagaaacaat cttaaaagct gtacgcaatg ctgtagctgt taatgaagtc aatgcaaaaa
    49021 tggggttgat ttgtgctaca ccgactgccg gaagcgcggg tgttgttcct ggtgtgctga
    49081 tggctgtccg agatcggctt gatttgacac atgaacagca attggaattc ttatttaccg
    49141 ctggcgcttt cgggttagtc atcgcaaaca acgcttctat cagtggtgca gaagggggat
    49201 gtcaagcaga agttggttct gcaagtgcaa tggcagcagc agctttggtt tgtgcaaaaa
    49261 atgggactcc tcatcaagca gcacaagcag tagccattac attgaaaaat atgatgggac
    49321 tgatttgcga tcccgttgca ggcttagttg aagtcccgtg tgtcaaacgt aatgctttag
    49381 gctcttctca agcttttatc tctgctgata tggcattagc gggtatcgaa agtgtcattc
    49441 cgccggatga agtcgttgct gcaatgtacc aagtcggacg tcaaatgccg tcgattttca
    49501 aagaaacagc agaaggtggc ttagctgtta cgccaactgc taaacgactt gcggaagaga
    49561 ttttaaataa gagggcgtga taaagctcct taatttcgag tattaagaga ttagttatct
    49621 tcataacaaa aaacgaagag aggactaatt gcccttctct tcgtttttta ttaatccatc
    49681 aatttcgtat tttttacttc atcgatcgtt tttgttcctg caagttgcat cgtgatcgac
    49741 aattctttgt tcaggtgttc aaaaacagat gttactcctt ctgccccacc tagattcaac
    49801 ccataaatca ctggtcggcc aatcgcaact aagtcagcac cacttgccag agctttgaag
    49861 acatgctctc ctcgacggac accagagtcg aaaatcactg gtacacgttt gttgactact
    49921 tcggcaattt ttggcaatac ttcaaaggac gcaggacctc catctagttg acgtccacca
    49981 tgattggata cccagatacc atctgcccct gcagagatag cgattactgc atcttctggc
    50041 gattgtattc cttttacgat aactggaaga tgggtgattt ctttgattgt tttgatgtca
    50101 tctggtgtca agccttgttt tgcagctgca taaatttctg cgattccttt tccttcgcca
    50161 ttaccacttg cactttgttc gctatacgca gctaagttag gcattggtaa tggaaattgg
    50221 aattgattga tgacgtcttc ttcacggtaa ccaccgagcg ttgaatctgc cgttaaaatg
    50281 atagctttag cccctgcttc tactgctttg ttcaaaatga actcattgaa tctgtcgtct
    50341 ttactcatat ataattggaa aaactgcggt gcgcctggcg ctgcatcggc tgcgtctttg
    50401 attgtcgtat ttgcataagt gctgatggaa aagattgatc cagcagcagc tactccttta
    50461 gctgtatcag cttccccttt ttcatgtgcc aatccttggg ccgctgatgg cgcttggata
    50521 atcggtgtat ctaattcaat tccgaaaaca gatgtatgca aatcggctga gtcgattccg
    50581 cgtaatatcc gtggcatgat tttctttgta ttaaaagaag tcgtattttc tttcatcgtc
    50641 cactcatctt cagaaccacc gcgaatatag ccaaaagctc ctgcttccat gcgctctttt
    50701 acacgacctt ctagttaggc aatgtttaca atatcgattg gatgttcttc attacttgta
    50761 tgataagtca tagtagatcc ttctttcatc ttcttgatta catataaatc atactaaact
    50821 tacaaaaagt aagcaaacat ttactctcag atacttttat cgctattctt tccaaataaa
    50881 tcgacttaca agctgatcta cttcttggtt ttcatgaagc atactatgag aggcattttc
    50941 acctgtcact actttttcct ctatttcatt tccgcgttgc ttcaacaacg catagaccgc
    51001 taatgcactg tttaacggta ctgttccatc actcaagtct gtttcatcta gttgaccagc
    51061 aatcaagaaa aatcgagtgg ttataggaat attaccgatc atttgctggt aatcttgata
    51121 gcggttagaa acgacagcag gcccctttgc taactcatct gtcagagact gcgcactatc
    51181 atcgacaaaa tcattaaaag gcgcgccaat tgctgttaat gtctgaactt gtggcaatga
    51241 cgtatcctgt ccatacgttc ctaaatagcg tagtgcacta actcctccca tggaatgccc
    51301 cactatgttg actttatcta tgccgtagtt ttctttcaga tacaaaagac aagttttgat
    51361 ccattctgct tgattccact cgttattttt attatcttca aacaatactt ggaccatcgg
    51421 attgtctgct tttccagaca aatctcctgt tgcttgtatt tgtccatctg ttcctactgt
    51481 taaaaccaat tcttttactg cttgctgatt tttttccaaa cgccgcaaca tccctttaaa
    51541 ggaattttct gtcccgctgt agccgtggaa aaaaagtgtt ggaatagtgg ttttttctat
    51601 cactttactg gattctgccg ttttttcttt atcagaaact tcagtagtat ttatcttcgt
    51661 cgtctggcaa ccacttaacg caaacaaact gataaggact aaagctgctg cagttgcacg
    51721 tttgcataaa tttttgattg gttctgtcat acacctcttc ctttccatat agaaaaaaga
    51781 aagacttgaa aggccaatca tatgatcgac atttcaagtc tctttattaa tcgattttat
    51841 ctacttgatc gaaatcaagt tctgtactgg tttcacgtcc aaacatatcg atgttgactt
    51901 tcaatttttc tttttctccg tcaacttctg ttacttgtcc ttctaatcca gcaaatgcgc
    51961 cttcgatgat tttcacgact tcgccaactt ctacttctag atcattttgt cgcaggctca
    52021 taccgataga acgcaagata tgattgactt cttctggaag taatggtgct ggtttgcttc
    52081 cggctccgtg tgaaccaacg aatcctgtca caccaggtgt gttgcggaca acataccatg
    52141 aagcgtctgt catgatcatt tcaacaagta cgtatcctgg gaatgtttta tgaacgattt
    52201 ctttttcttt cccatttttg acttcagttt ctttttcttc aggaacaacg acacggaaaa
    52261 taaagtcggc cattttcata ctttgcgcac gagattcgat atttgctttt actttgtttt
    52321 catatcctga atatgtatgc aagacatacc agtttttttc aaaagattcc attgaaggct
    52381 cctcatttca ttgtaaaata aaaaaacctc agtacacccg aagtttttct ttcgcttcat
    52441 tatagcacta aaagtacgtt gtggctagcg agttatttta aaatccagcc aaataagctt
    52501 tggatggctg tatccattac gtaaaacatc aatccgaaaa taatagaagt ttcgatgact
    52561 actaacgtat ctttacggag ttgttttctg cttggccaag ttacttgttt gatttcatct
    52621 tttacgcttt ttagaaattt catgagacaa cctcccttgc tactgtttat ttcgtttctt
    52681 tatgtaatgt atattgatta caatatttac aaaacttatt gatttccaaa cgttcgccgc
    52741 tttttccttc actgactgat tttgtgtagt tgcgagaccc acaaacggaa caagcgagtg
    52801 ctgctttttt ctttgccatt tacttctgcc cccttgctga gaatcagact tatacttcat
    52861 aacagtatca cgtaactttt tacttgtcaa tttgtgaaat aaactatcta ttgttttgga
    52921 taaaattatg atatgattaa tttgaaatta agctaaaatg agacaattgg aggtatagcg
    52981 atgctattaa aaacacttgt atataagaaa aaagatctga caaccgttac agagactagt
    53041 acattagaag aagcattaaa aattttagaa acttccggat accgttgtgt gccgatctta
    53101 gattcttcag gtaatatttt ccgtggaaac atttacaaaa tgcatatcta tcgtcataaa
    53161 gcaaatggcg gtgatatgac gcttcctgtc actcatcttt tgaaaaacgc tacaaagttc
    53221 atttacctgg atacttcttt cttcaaagta ttctttacga tcaaagagct tccttatatt
    53281 gctgtccttg acagtgaaaa tcatttttat ggtattttga cacatagtac gttgcttaat
    53341 atgctctctc aatcttggag cgtagaacaa ggaagctatg tactgacaat tgcttccaca
    53401 ggaaagcaag gagatttggc tgctattgca aaaatcattt caaaacacag cagcattgcc
    53461 agctgtatta ctcttgatat cgaaaaagac gaatacattc gacgtacatt gatcacgcta
    53521 ccggctaaca ctgaagcgaa agtatgtgaa acgatcgtca accacttaga gaaaaagaat
    53581 tataaagtgg tagaaattga aaatttgcaa aaccaagaag cataatcaaa aagcgaaaaa
    53641 agaaaacagc taatctgctt tcttttttcg ctttttataa ttagacacgc ttttcaaaat
    53701 caagcgcgcg atctaaataa tacatcaatg gtgctgaaac agccatgacg ataaactcac
    53761 ttaatgcaag cgtgccatag gttgtccaaa atgcttggcc acctgttgga actaacatat
    53821 aagcaatcag tcccatgctg atggtaaaga agatcgtgtt caatactaaa cgcatcttta
    53881 catttttcac tttgttttgc aataaagctg tcaggcctaa agaaagcaat gtttggccgc
    53941 caccaaaaac cacatccatg ataccaaaac caaagaaggc attatagacg atgaccccgc
    54001 ctaaaactcc ccaaagcaat ttacggttaa agacaactaa atgattcaaa ctttctgaaa
    54061 tacgaaactg gatcggccct gaggatacag gcgcaactaa aatagttaac gctagataaa
    54121 gagccatgat gatgccattt acaacgatta cggataaccg ttgtctgctt ttcgtttttt
    54181 gcattttctc tcctcttttc tcctagtttt tttaacgcgg gatggttgat gaaccgcggt
    54241 tatgtgaaac aaacaataat tataacagac ttttcattta tttcaatccg tcttatctgc
    54301 ttttaaaaaa gcaaaagctt aaacaatgta aacgtttgct aaaaatcgtg ggatatggta
    54361 taaagtaagt gtaaggggtc ataagaattc ccagtaaagc agacgaagta ggatctgcaa
    54421 gaacagaatt gttgtgaaac ttaaataaat aaaaaaggat gtgggaattt gagcaacctc
    54481 ttactagaga acggtctagc aagcagctga agatctaagt ggacatgccg aaccttggaa
    54541 cttctcctta cgtaagtaat ggatcatgcg cttgctaggc ttttttgtgt ttttcggtct
    54601 tcggaccttt ttcattttcc tagacttctg ctataataac caagaattaa ttttgtaaca
    54661 gatttttttg aaaaaacaag tttctttcat tcaaaaaata gaaaaagctt gctaattatt
    54721 ggttaaccag caaacaggag tattaaaata tcttgtttta ttgaatagca ggaggaaatc
    54781 atgaaattcc attatcatat attgatcaat ccatcggcag gcagcggaaa tggctataaa
    54841 gtagcagaaa gaattttacc ggtattaaaa aataagcata ttgactatac acttcattat
    54901 agtgaataca aaggacacga tgctgagatt gctgaaactt tggcaaaaga aacattgatt
    54961 ccttggatag aagaagaaca gaaaacaatc gatactttcc cccttttgat catcgtagga
    55021 ggtgacggca ccttacatca ggtacttgat actttctatc agatggaagt agaattccca
    55081 gttgcttata ttccagcagg ttctggagat gattttgctc gaggggcagg ccttccaaaa
    55141 aatcccaaaa aaggacttca gctgatttta gcagctcaat caccagaaaa agtccacata
    55201 atggcttatg aagaaaaaat aagtgaaaaa aaaggaatag ctgttaataa tttcggtata
    55261 ggattagatg cagctatcgt ccatgcgaca aatcattctt ccacaaaaaa gcgattgaac
    55321 aaatacaact taggatcatt ttcgtattta ttctctattc tccgtgcttt attcactcaa
    55381 aaaggatttc caattctggt agatgcagga ggaaaacaat taagtttttc aaacgcattt
    55441 ttatgtacag caacgaagca tccttttttt ggcggaggaa tcgcgattgt tccaactgct
    55501 gatgcgtcta aaccagtcat tgattttgtc gtggtcgaac gaatcaattt ctttaaaatc
    55561 ttgtggctca ttcttttact tatacagaaa aaacaattga aatcgaaata tttccatcac
    55621 tacacaacaa acagattacg tatcgtttca acaacaccgc aacatggtca agaagatgga
    55681 gaagaaatgg aaaaacgacc ttttgatatc acaataacca caactaaaca gttgttttgg
    55741 tgttaataaa agaggttttg tcagaagcta catctggaac accgtttatt cggaaaaaag
    55801 gactgtgaca cgacttttgt cacagtcctt tttttactat gcagttaatt ctttgcccaa
    55861 ttgggttgcc atcgatagtt tctttcttta cccaaaataa attggatcat tgaaaacacc
    55921 gcagcaaaag caactaagac acaaccattg aaccaaaaag attctgcaaa tgtttgattg
    55981 taactgagtg tttcactgat caagttatta ttttgaacaa accaatatgt tggtgtgaat
    56041 gccgctattt tgctaaccat ttctggcaag tattctgctg gtacaaacac cccaccgata
    56101 aagcaactgc ctgtgataaa aatatctttg atcccaccgc tggtttctga gttttttacc
    56161 aatgaagtga tacaagccga aaagctaatg atcggcaata aaaacaagaa agtatttaaa
    56221 atatgatacc ataccgttat atcccacgag gtgtggatca ctaacagaac cattagaagt
    56281 aagaagccac tgaacaacag cagcgagtaa ctaatcaaac taaaggttac tttgcgattc
    56341 aagtttcgtt tggtaatggg cgcactctgg tttcgtttac tgatctcttt gcgattaaat
    56401 gctagattga tgacgctgaa accgctaaaa atggatagaa acaacccata agacaagaaa
    56461 ttaaaggttg tgcccgtcaa cagctttttc atccgctgac tatatcccgc acttagctca
    56521 acttctccag taaccgagag tgccatttgt gtctgttcca tagctgcatc gattgtttta
    56581 tcttcttctt gaaacagaat cagtgtttgc aaaaaggtgt tcacttgctg tgtaacaact
    56641 gttttagaaa aggcatctgg gcgactctgt atcttgatcg tcggcatctt tcctacttcg
    56701 acttgttcgg taaagtctgc aggcagatgt aagataatgt ttacacgatt aaaataaaga
    56761 gcatcatcga tttctttttg tgaggttcca tccaatgtaa cgactgtttc cttgtcattc
    56821 aaatatttac gaaggccatc gactacaggc gactcttctt cactaatcaa ggcgattttc
    56881 gcatcttgca gagaggtatt ttcaacatca atattttgcg caaaaaagaa tgtggcaaca
    56941 acagtgataa ttacacctat cagtaaattc gatttatttg ctttaagcaa tgtccaaatc
    57001 gttttataaa gcgtcataac gcacccctct ttccatccaa gcatttccta acgcaaaaag
    57061 gaaaataaat ccgatcaacc atagaagatt ggtataaaaa ctagccaaat cagtgtagaa
    57121 ataaagttta ggcggatttt ataatgaact tagccattta aaataaaaaa aacgccacgt
    57181 gaattccatg ttatattgaa gttaccacaa ctctcaatag acaaggatga attcacatga
    57241 cgcaaaccca tgataacaca gtttcacgca agggaaaaca tctttcctac tcagaacgtt
    57301 gccaaatcgc tatcctgaaa aaggaaggct attccaaccg tcaggttgcc aaagtgctgg
    57361 gacgtgttcc gcaaaccata aacaatgaga ttgatcgcgg aaccatcacc cagttaaaac
    57421 gccaaacgca aaatggaaaa gtgtatgaca cctactattc tgtttatgac gctgatgcag
    57481 gacaggcctt ctatgaaaag caacgactga actgtggcag acgccccaaa tgggcgaaca
    57541 caaatgcctt cattgattgg gcagatgaca aaatgttaaa tgaaaaatgg tcgccggacg
    57601 tcgtggtcgg atttgccaga aaacacgagc tgttcgattg tgccatcatt cctggcacaa
    57661 ccgctcttta tcaatggatt gaccgcggaa tcatgcgaac aaagaacatg gacttgcttg
    57721 aaaaactgtc ccgtaaaatc aaagcggtcc gcccaggaca ccgtcccaac aagcgtgttc
    57781 tcgggacgtc aattgaagaa cgccctaaag aagttgaaac acgggaaacg ttcggtcatt
    57841 gggaaatcga cacggtagtg ggaaacaaga ccaagtcgga tgccgtgctg ctgacgctgg
    57901 ctgaacggca gacacgcttt gaagtcatcc taaagttgaa cgggaaggac accgaatcgg
    57961 tggaccaagc ggttcaacag ctaagagaac gggctggcgc taacttcgat caattattta
    58021 agaccgttac atccgacaat ggctcggagt tttctgggct acacgatgct ctgcaagatg
    58081 tagctgacgt ctatttcagc cacccctatg cttcttggga gcgtgggacg agcgaaaacc
    58141 agcacaaatt aattcgccgt ttcgtcccca agggaagccc gattagtgag gtgagcgaga
    58201 accagatttt gcggatccag cgttggatga acgattaccc gcggaaaatt ttgggttatg
    58261 cgacaccaca tgatacattc gtccaggcat tcaagcaaga acggttggtg gcttgaccca
    58321 ttattccttg ataacgatcc tcactcggga catggcttca tgtctggaaa gctggcctgg
    58381 ggccagaatc gtctgaagac tatcatacct cagttcatta tcaaggcctc actgatttag
    58441 aataacgagg cagttacggt atcaaacaaa tgagttacct gt
//
LOCUS       DAYYFU010000013        51693 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000013 DAYYFU010000000
VERSION     DAYYFU010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.013"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..1495
                     /locus_tag="ACHA34_000941"
     CDS             68..1495
                     /locus_tag="ACHA34_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8791604.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1587..1952)
                     /locus_tag="ACHA34_000942"
     CDS             complement(1587..1952)
                     /locus_tag="ACHA34_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8791605.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1949..2314)
                     /locus_tag="ACHA34_000943"
     CDS             complement(1949..2314)
                     /locus_tag="ACHA34_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8791606.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2506..2754)
                     /locus_tag="ACHA34_000944"
     CDS             complement(2506..2754)
                     /locus_tag="ACHA34_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791607.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2753..2881
                     /locus_tag="ACHA34_000945"
     CDS             2753..2881
                     /locus_tag="ACHA34_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFM8791608.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2938..4632
                     /locus_tag="ACHA34_000946"
     CDS             2938..4632
                     /locus_tag="ACHA34_000946"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFM8791609.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4732..4905
                     /locus_tag="ACHA34_000947"
     CDS             4732..4905
                     /locus_tag="ACHA34_000947"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791610.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5283..6416
                     /locus_tag="ACHA34_000948"
     CDS             5283..6416
                     /locus_tag="ACHA34_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFM8791611.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6447..6767
                     /locus_tag="ACHA34_000949"
     CDS             6447..6767
                     /locus_tag="ACHA34_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFM8791612.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6796..7260)
                     /locus_tag="ACHA34_000950"
     CDS             complement(6796..7260)
                     /locus_tag="ACHA34_000950"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8791613.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7392..8645)
                     /locus_tag="ACHA34_000951"
     CDS             complement(7392..8645)
                     /locus_tag="ACHA34_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8791614.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8674..8991)
                     /locus_tag="ACHA34_000952"
     CDS             complement(8674..8991)
                     /locus_tag="ACHA34_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8791615.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(9017..9337)
                     /locus_tag="ACHA34_000953"
     CDS             complement(9017..9337)
                     /locus_tag="ACHA34_000953"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8791616.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9801..11216)
                     /locus_tag="ACHA34_000954"
     CDS             complement(9801..11216)
                     /locus_tag="ACHA34_000954"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8791617.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11233..12549)
                     /locus_tag="ACHA34_000955"
     CDS             complement(11233..12549)
                     /locus_tag="ACHA34_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8791618.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12699..13397)
                     /locus_tag="ACHA34_000956"
     CDS             complement(12699..13397)
                     /locus_tag="ACHA34_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFM8791619.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13686..14675)
                     /locus_tag="ACHA34_000957"
     CDS             complement(13686..14675)
                     /locus_tag="ACHA34_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8791620.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14755..15588)
                     /gene="msrA"
                     /locus_tag="ACHA34_000958"
     CDS             complement(14755..15588)
                     /gene="msrA"
                     /locus_tag="ACHA34_000958"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8791621.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15763..17397)
                     /locus_tag="ACHA34_000959"
     CDS             complement(15763..17397)
                     /locus_tag="ACHA34_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFM8791622.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17384..19072)
                     /locus_tag="ACHA34_000960"
     CDS             complement(17384..19072)
                     /locus_tag="ACHA34_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791623.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNI
                     CTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYY
                     AYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFV
                     VVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDI
                     TTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLL
                     QFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVI
                     QSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKA
                     ADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIE
                     ITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            19218..20855
                     /locus_tag="ACHA34_000961"
     CDS             19218..20855
                     /locus_tag="ACHA34_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791624.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(21000..21740)
                     /gene="truA"
                     /locus_tag="ACHA34_000962"
     CDS             complement(21000..21740)
                     /gene="truA"
                     /locus_tag="ACHA34_000962"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8791625.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(21931..22566)
                     /locus_tag="ACHA34_000963"
     CDS             complement(21931..22566)
                     /locus_tag="ACHA34_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM8791626.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(22713..23933)
                     /gene="sstT"
                     /locus_tag="ACHA34_000964"
     CDS             complement(22713..23933)
                     /gene="sstT"
                     /locus_tag="ACHA34_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFM8791627.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            24152..25150
                     /locus_tag="ACHA34_000965"
     CDS             24152..25150
                     /locus_tag="ACHA34_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791628.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            25346..27181
                     /locus_tag="ACHA34_000966"
     CDS             25346..27181
                     /locus_tag="ACHA34_000966"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8791629.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(27241..27957)
                     /locus_tag="ACHA34_000967"
     CDS             complement(27241..27957)
                     /locus_tag="ACHA34_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8791630.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(28184..28726)
                     /locus_tag="ACHA34_000968"
     CDS             complement(28184..28726)
                     /locus_tag="ACHA34_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFM8791631.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(28731..28958)
                     /locus_tag="ACHA34_000969"
     CDS             complement(28731..28958)
                     /locus_tag="ACHA34_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFM8791632.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(29037..30917)
                     /locus_tag="ACHA34_000970"
     CDS             complement(29037..30917)
                     /locus_tag="ACHA34_000970"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8791633.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(31106..31720)
                     /locus_tag="ACHA34_000971"
     CDS             complement(31106..31720)
                     /locus_tag="ACHA34_000971"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8791634.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            31862..32422
                     /locus_tag="ACHA34_000972"
     CDS             31862..32422
                     /locus_tag="ACHA34_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8791635.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(32727..33041)
                     /locus_tag="ACHA34_000973"
                     /pseudo
     CDS             complement(32727..33041)
                     /locus_tag="ACHA34_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(33295..34930)
                     /locus_tag="ACHA34_000974"
                     /pseudo
     CDS             complement(33295..34930)
                     /locus_tag="ACHA34_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(35485..36048)
                     /locus_tag="ACHA34_000975"
     CDS             complement(35485..36048)
                     /locus_tag="ACHA34_000975"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM8791636.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(36063..37106)
                     /locus_tag="ACHA34_000976"
     CDS             complement(36063..37106)
                     /locus_tag="ACHA34_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791637.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(37239..37643)
                     /locus_tag="ACHA34_000977"
     CDS             complement(37239..37643)
                     /locus_tag="ACHA34_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8791638.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(37876..38511)
                     /locus_tag="ACHA34_000978"
     CDS             complement(37876..38511)
                     /locus_tag="ACHA34_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM8791639.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(38777..39829)
                     /locus_tag="ACHA34_000979"
     CDS             complement(38777..39829)
                     /locus_tag="ACHA34_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8791640.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(39842..40780)
                     /locus_tag="ACHA34_000980"
     CDS             complement(39842..40780)
                     /locus_tag="ACHA34_000980"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8791641.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(41231..41881)
                     /locus_tag="ACHA34_000981"
     CDS             complement(41231..41881)
                     /locus_tag="ACHA34_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791642.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(41981..42490)
                     /locus_tag="ACHA34_000982"
     CDS             complement(41981..42490)
                     /locus_tag="ACHA34_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFM8791643.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(42691..43560)
                     /gene="scrK"
                     /locus_tag="ACHA34_000983"
     CDS             complement(42691..43560)
                     /gene="scrK"
                     /locus_tag="ACHA34_000983"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8791644.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(43580..44701)
                     /locus_tag="ACHA34_000984"
     CDS             complement(43580..44701)
                     /locus_tag="ACHA34_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8791645.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(44703..45098)
                     /locus_tag="ACHA34_000985"
     CDS             complement(44703..45098)
                     /locus_tag="ACHA34_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8791646.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(45117..45914)
                     /locus_tag="ACHA34_000986"
     CDS             complement(45117..45914)
                     /locus_tag="ACHA34_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8791647.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(45916..46704)
                     /locus_tag="ACHA34_000987"
     CDS             complement(45916..46704)
                     /locus_tag="ACHA34_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8791648.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(46718..47194)
                     /locus_tag="ACHA34_000988"
     CDS             complement(46718..47194)
                     /locus_tag="ACHA34_000988"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8791649.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(47266..49923)
                     /locus_tag="ACHA34_000989"
     CDS             complement(47266..49923)
                     /locus_tag="ACHA34_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8791650.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(49987..50346)
                     /locus_tag="ACHA34_000990"
                     /pseudo
     CDS             complement(49987..50346)
                     /locus_tag="ACHA34_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            complement(50524..50844)
                     /locus_tag="ACHA34_000991"
                     /pseudo
     CDS             complement(50524..50844)
                     /locus_tag="ACHA34_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(50960..>51693)
                     /locus_tag="ACHA34_000992"
     CDS             complement(50960..>51693)
                     /locus_tag="ACHA34_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791651.1"
                     /translation="LCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLKNGKSDLTHS
                     IKKMNSLRVAKAIVGMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPHEDYKIIMNY
                     YLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQYEGIGFTLID
                     LKNSTFEIKEGLDLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDL
                     LIRWQVITNFLMELKKYSNTQTKFS"
BASE COUNT        16335 a        10196 c         8105 g        17057 t
ORIGIN      
        1 ttcaaaaaga aaacatgtca taatatttcc catattgtgc aaaaaaatac aaggcggagg
       61 ctctactatg attgaagaat atattgagaa aaatatttta agacagctgt ttttatgtgg
      121 gcaattctat gtaaataaag aagtcaatct agaaaagctc tccaatctcc tgcatgtttg
      181 caaaaccact ttactcaatg atatcaataa cataaaaaaa gaatttgaag aacagatcgc
      241 ttatacacat cgggaaaagg accgttacac tttatatttt tcagaacata tacctcgatg
      301 caagatcatg cagcaattgt ctcaaaattc gttgttttta aaaacttgtc tgctttattt
      361 ggaagaagat gaacctgatt atcttcaatt aactgaatgt gaattcatct ctgtcagcaa
      421 agcttattca ttaaaaaaac aagtattagc ttactttaat gactgcggga tcgaaatcga
      481 ccgctactcc cctcgattca ctgaaatgga aagacgtctg ctgcttctta atgtgtcgta
      541 tcgtttaggc ggttttaatt cttgggagct acctgaatcg ttttttgaac gtgccgatcg
      601 tttcattgaa tccgtaacgg aaaatagtgg cagattttat gataaagaaa ataaagaaat
      661 cttatcgatt ggttttgcta tcagtttcct acgccagcaa gtgtgtgctg tgacaataga
      721 ttcaaaattt atcgaagaaa tcaaaaaacg tcctatttac aattatgtcg aatcagcatg
      781 ggaaaacact gatttccaaa cctattataa aaaagaagaa ttcgccttta ttttgacact
      841 attcaatctt tgtaattatg gtttccattc ttatcagttg attgcggaag actttcaaca
      901 gctacaccaa gtgtttattg acaatactcc cgaaataaaa gaactagttg ctacctttga
      961 atctcacttc aatcaagaat tattcggaaa ccaaccgttc gaacgtgcgc tgattcatct
     1021 gatgcgttcg gcttgggata actatcaatt attcatgcca gaaaaatttt atcttttaaa
     1081 tgaggaacag acaaatttat ataaagaagt ccagacaatt ttttccagct ggtccagcca
     1141 gttaccttat gatctacggt tgaatcctaa ttgcatgcgg gcttttgtga tcgaattgag
     1201 cggaattctc cgtctgacaa aagaacatct gactatctat atcgtcacaa attcggatgt
     1261 tcattatctg atttatcggg aagccttgga agctgtaact actttcgatt ttcaagttgc
     1321 accaacaatt tattcaagta tatctgatat aaagaagtat gcgcagcagt cctcaaaccg
     1381 ggtattatgt gaacgtacat tatatacacc agatgcggtc caatatgaaa atattattcc
     1441 tatttcgatc aatacgatcg atcgagctat catttcagct gtgcaaaaca aataaggggc
     1501 tgtgacaaaa atcatgtcac agcctcttat ttgttgaaga tgcccatttc tgtaacaatc
     1561 tcttgataca cgtcaggctc acaacctcac ttattttgga catctagggg tccgatctct
     1621 ttatttaaaa aataagaaat gatggttcgg ataactacca acgccgctag ttggaaaata
     1681 tcctgcaagg taggtttgat aatcgattcg atgatcccag ctgaaacaag aatttccaaa
     1741 cttagaagta tatagcctcc caacatcttt ttaatagcat tgttctcgct tactcggtcg
     1801 ctttgcactt ttgtatggac aaataaatat ttcaaaaaat ccttgattga tagacaaaca
     1861 ccccaaacca ataaacagat agcaaagata tcaagtccca gtacaagttt ttcgaaatat
     1921 ggaagaagaa actgcatcaa ctctgtcgtc atcctacctc tcctcttctt tgtttttctt
     1981 tgtcttctat ttcctgattc aagaaataag aaatgactgt tcgaatgata ataataagtg
     2041 caagcttcaa aatatctgtc caagtcggct tgataactga ctcgatgata tcagcaacga
     2101 ctagtacttc caagcttaac aaaatataac ttgctagcag ttttcttgta tctgcattac
     2161 gagtattacg ttctgcatag tttttcttga catgagacca atcaaaaagt ttgaatagaa
     2221 caagaacgat cccgacaatt aagataccga tagaacaaat atttaaaata gcaatgatcc
     2281 attctagaaa ttcaattaaa tttgagcgaa acatatacgc tacctacttt cttttaattt
     2341 gttcctattc ttttattgta aaagaaagtt accttgttcg tctatttaaa agttgttcta
     2401 atgataacgg ttcgcttaaa tttttctttt tcactctccc atttttctcc ctccatccat
     2461 aaatcgtgga agtatccaaa acaacgtagc aagttaaaat aaggcttagt ccgtcatcag
     2521 cttttcaaag aagcactgtc tcggtgcttt ttttgtaccc aaatttctaa aaatagaacc
     2581 atacacctcc tcaaatcttt tcttcttacc taccctattt ttatttacag gaaaatcaga
     2641 cacttcttct tttttgtcaa aatttccttt ttcttctcgt cctatcattt ggcattctaa
     2701 taaaaacgaa ctatccttgg atatagaaag agaaaacaag gaggcgtttt tcatgagtaa
     2761 aaaattaatc aaagtcggca tcggtttagg tttgcttgcc cttggcgctg catatctagg
     2821 aaaaaagaca ggactattcg aagatgacag tcatttatat gatgaattcg aatcaatcta
     2881 gcatcctatt tagttgaatc actacggaca tacttggaag acattggagg aatcacaatg
     2941 agaaaaagta aagcaataat gattggtgcc ggtattgcca atatggcagc ggctgtttat
     3001 ttgattcaag aaggcaaatg gaaaggggat cagatcacct tttattcatt ggatgatcac
     3061 ggttcaaatg atggtgcacc gaccgacact gtagtggatg aatattggaa taaaaaccat
     3121 ccattagaaa accaaaaagg ctatattgct cgaggcggtc gcatgcttaa ttaccgtacc
     3181 tatgtagatt tgatggatct gttaagtcga ataccttctg ttactgagcc agggatgact
     3241 gctgaagaag atacgagaga ttttgatgca aaacaccgaa cctttgacaa agcacgtttg
     3301 atggaaggtg gcaaaggaat cattgatggc ggaaaactgg gattcaataa taaagaccgt
     3361 attcttctga ctaaactgat tgctatgcct gattcagaag aagaaaaact agacaatatc
     3421 actatcgaag aatatttcaa agatgatcca cacttttttg aaacaaactt ctggtttatg
     3481 tgggaaacaa catttgcttt cagaacacgt agttctgctc aagaattgcg tcgctatatg
     3541 catcaaatga tttatgaatt cactcaaatc gaacatctag tgggcgtcaa tcgaacacgt
     3601 tacaatcaat acgaaagtat tatgcttcct ttgatcgagt acttgaaaga acaaggatgc
     3661 aaaatcattt tgaaccgccg tgtcactgac tgggaattca aagaaacacc gatgcaagat
     3721 gaaatcactg tgactggttt gaaaattacc aatacactga ctcaagaaga agaatacgtt
     3781 gtggtcgatg aagatacagc agttattttc actaatgggt ccattacaga ttcagctact
     3841 ttaggagatt acgaaacacc agctgtagaa aatatggatt atggtgcagc ttctagtctc
     3901 tggaaaaaag ccagtgaacg tttctacaac ttgggaaatc ctgacaaatt ctttgctgat
     3961 cgagatgcaa gcgaatgggt cagctttacc ctgacgacga aaaaccattt gttgttgaat
     4021 gaaatcaccc gtatcacgac tcaagaacca ggaaatgcct tgaattcatt tatttcaacc
     4081 acaccgatca cgccgttagg ccaaaaagat gtcaatatgt ctattgtcgt gcatcatcaa
     4141 ccgcatttta cgtctcagaa accaaatgaa acagttattt ggggttattt cctttatcca
     4201 agacgacgtg gcgaatttgt ggataaacag tatattaaaa tgaatggaaa agaaatgcta
     4261 gaagaactga ttggacaact ttcaaaagta gatcctggac cagtgaatat tcgtgaaaaa
     4321 gaaacagaaa ttttcgacag cgtaataaat aatatccctg tctatatgcc ttatgcttcc
     4381 gcgttgttca ataatcgtgc caaaagcgat cgaccaaaag tcattcctaa acattcgacc
     4441 aatctggcct ttacaggaga atttgtcgag cagccttatc agatgatctt tactgaacaa
     4501 agtgctgtac gttctggaga aatcgctgct ttccattttg cgggtattcc tatgtcccgt
     4561 cttgtcaaaa caccgcgtta cgacaaagat attcctactc tagctcgtgc cgcaaaaaaa
     4621 atgtttgaat aatcaagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacaacc
     4681 aggctaaaac agcttcttat gtttttgatt tggttggact tattaccgca gatgcaagct
     4741 tttgaacacc gtttaccaag aggttatgaa aaagctgccc tgcatcaagt aataagagca
     4801 gccaagctaa aacagcttct tatgtttttg acttggttag acttattacc gcagatgcaa
     4861 gcttttgaac accgtttacc aagaggttat gaaaaaggcg tttagtttcg agtatgaaga
     4921 aaaattttgt gaaaatggtt cctcacgttt ttgcataaat ttgacttaat accgagaaac
     4981 tgatcccttc tgtcataacc tctttttatt tatgtctgtt tctttttgaa aacaactatt
     5041 ttttcaacgt ttactctctt gccaatccaa catcatctaa cggatacaac tatttcaatg
     5101 attaccatta cactaaaaaa tcggaaacac acctctccga acacaaatac ataagactat
     5161 agaatgtttc atttaagaaa aaaagcgttt cattatcaaa aaggtaagat ctcctttaaa
     5221 atgaaattat ctgtagtact aataatgcag acaattttat gaatctgaaa ggagcatctc
     5281 caatgaaagc agtcacttgg caaggaaaac agaaaatgga aatcagaaac gtagatgatc
     5341 caaaaatatt agaaccaaca gatgcaatca tccgtatcac agcaacagcc atttgcggct
     5401 ccgatctgca tctttaccat catggtgaag cagttttgga aaaagggtat gttgtaggac
     5461 acgaaccaat gggaatcgtt gaagaagtgg gatctcaagt tcaaacattg aaaaaagggc
     5521 aacgtgtggt tatccctttt aatattagtt gtggtcattg tcatttctgc ctgaatcaca
     5581 tggaaagcca atgtgatgaa tcaaatccag atcagttata tggcgggctt ttcggatttg
     5641 gcaagttgaa cggtaatcat tggggtggtc aagcggaata tctaagagtt ccttatgcag
     5701 actctacttc atttgttgta ccagataatg atcttcctga tgaaaaagtt ttatttctat
     5761 cagatatttt gcccacagct tattggagcg ttgaaaacgc aggagtaaaa aaaggcgata
     5821 cagttgtgat tctaggttct ggtccagtcg gtctattcgc tcaaaaattc gctgttatgg
     5881 ctggagcaga gcgcgtaatc gcagtagatc cagtacagca ccgtctagat aaagcggcta
     5941 attacaatgg ggttgaaact tatttcttag aagacacggc acaagctgga acagaattgt
     6001 atgaactggc taaaggtggc gcagatgtca ttatcgattg tgtaggaatg gacggattag
     6061 agcctgtcaa agaaaaagcg aaaaatttag taagcttaca aagcggcaca atctctccat
     6121 tacagatggc atcgcaagct gtcaaaaaat ttggaacagt ccaagtaact ggtgtttata
     6181 tgactccagc ttcttcttac ccgctgcaag aattttttat gcgggatatc gctgtcaagc
     6241 atggacaggc accaattatc catctaatgc caaaaattta cgcaatgatt gcagaaggac
     6301 tattcgatcc tacacaaatc attactcatt cgctgccact atcagaagca gcacaagcat
     6361 atgagatctt tgacaaaaaa gaagataaaa atataaaagt tgttttgaaa ccttgaattc
     6421 agtaaaaaat acaggtgatt ttctttatgc ccaaaatcaa acgaaatacc catgagatcg
     6481 aaataggaaa agaagaagat atcgaaatac gaggacgtcg cttccgcttc tatttccaaa
     6541 atcgttacac attgatctca ctggctgtcg atttattaac cgggattttc tatatttttg
     6601 gaagccttgc ttccttaact aaaatccccg atcgatatgg cacctatttt tatttggctg
     6661 gtgctatttt cttgacgatt cgtcctattc tcagaatcgt aaggaacatt tttatttacg
     6721 acgaaaaaaa agcaatgaag agaacaagca aaaaaaaaga ggcatgagaa tttttcaaat
     6781 ctcatgcctc ttttttcacc ctatcgtatc actttgatac agacgagcgg tcgtcccact
     6841 ttcttccttg accattgtca aaaatgtcca ctcgtatcgt tcataaaacg tggtatgatc
     6901 tgtaattaag taaagttttt cgtacccaag ttgatgcatt tcttttctga tttccgctaa
     6961 tatccgcttg gctatcccct gcttacgctt ttccggttca acaaacaggg cacaaagatt
     7021 tggtgtcaga tcagggcggt tatggaagtc gttttcaatg acgcccgctc ctccgacgat
     7081 ggtttctttt tcatctaaga ccacatacca ttgaggaata ctttccttat tagcgattcc
     7141 agtacgaata ctttcctcat acacaacagc aggaactccc cattttgatg aaaaccaatt
     7201 cgcgctttgt tttactaatt ctgggtgttc tcttaatttg atccagttat atgtaaccaa
     7261 tgtttaaacc tccgactaaa ataaaacgtt tacaaaaatt atacaagtct ttatgaaaat
     7321 tagcaatgac aacaatagtg atgtaaaaaa aaaaacggaa aacaatcact tgttctccgt
     7381 tttttgatta atcaagcatc taaagcttct tttcttcttt cttggatcgt agctaccttg
     7441 acgaacggta gatagatcaa aatagaaaga accaaattga cagcggctag tacaccacct
     7501 gcaatacttt ttgttgccaa tacaccgcca ataattggtg gagttaccca tggaggcata
     7561 acggtagctg ctggcactaa acctgtagaa gtcgcaaagt aagcaacact aactaagacc
     7621 attggtgtca aaataaatgg aatgaacatg atcgggttca acacgatagg taacccaaac
     7681 atcgttggtt cattaatatt gaagacacct ggcgcaatcg acaagtttgt tacaaccata
     7741 tatggtttgt ttttacgtcc tactaaataa atagctaaca gtaaacctaa agttgcacct
     7801 gttcctccta agttgacaaa gctgtcaaag aaaggtttgt tcacgataaa cggtgcgcta
     7861 tgtccagctg cgattgcatt aacgtttgct tcaatggctg gcgcattgat tgtttgcatc
     7921 aaaggatcga ccatattcgc tccgtgtaaa ccaaagaacc ataagaatgg tgtgataaat
     7981 gcaagcagta aggcagatgg ataagagttt gcaagtccca taaatggttc ttggaccact
     8041 gtatagaaag aacctacgat atcatcaaca ccgaatgcag caaaaattgc agcaactaaa
     8101 gcaaacgcag agatagtgat cattgctggt agtaatgaag caaatgcttt tgcaactgcc
     8161 ggtggcacac catcaggcat tttgatgatc aatttttcat ttccgcttaa acgtacaaag
     8221 atttctgttg aaataatccc aacaataata gcaataaaca aaccgtttga gctcatacct
     8281 aatgctccgc ctaaagcgaa gaaagagcct aaagaaatca cacctgcgga aacaccgtct
     8341 tttcggtagc cgttagctaa atgataagca agcaagaaag caatcaagac agaaagaatg
     8401 gcaaatgtac cgttccaagc agcagccccg aatcctttcc atgattcacc accaaaaatt
     8461 gagttcatca attcttggaa cccgggaatc ggtaagttat tgatcattac tgctaatgct
     8521 cctaagatca ttaacggcat actcaccata aatgagtccc gaatcgcaac taaatgccgt
     8581 tgtgcaccaa tttttgatgc tacaggtata aaatgtttct ccataaatgc aacaaattta
     8641 tccataaatc ttccctcttt tctttactgt agattacgcg ttcaatcgac gatacagatt
     8701 aatgatctct tttgctaaat ctgtaaaagc tatagatgtc atcagatgat cttgactgtg
     8761 gaccgtcaaa agcgtgactt caacgtgatt tccttgtgct tcttgtgtca gcatctctgt
     8821 ttgagagtga tgtgcttgga ctaaagattc ctctgcatct ttgatttttt catctgctaa
     8881 agtgaaatca ccttttttcg cagcagcaat cgcttccatt gcatcgcttt ttgcatttcc
     8941 gccaaacatg atcagaccca tgatggcttc tagatttttt tgatcttcca tttattgtat
     9001 cctccgataa tttttattat ttgtttatta gtttttctgc ttggtcgagt actttttcac
     9061 cattcatcat gccatagtca gccatattga ttacatctaa aggaatgcct ttaggtgcta
     9121 gcttttcagc aaattgattt tgcataaagc gtacttgcgg tcctaaaagt aaaacgtcta
     9181 catttttaga ttctagttga ttatccgcat ctgaagcaga tacagcaaag atgtctgctt
     9241 ccattccttt tgcttctgct gccttttgca tttttgtgac taggagactt gtactcattc
     9301 cagctgaaca tacaagcatg attgttttct tttccatttt aaacacgttc ctttctatac
     9361 ttacacttgt atataagcaa gtagcgtgcc aactttaaaa gcgctttaaa aagttgatat
     9421 catcacattt aaggtctata ccaagttgca tacacactaa aaaagaaagc gcacactaaa
     9481 cacacaaatt aatttttatt accttttttc ttttaacgtg cacaaaaaaa gttgtgacac
     9541 aaaacttgtc acaacttttt tcaaataaac ggtattcaaa agcttgcatc tgcggtaata
     9601 agtccaacca agttaaaaac ataagatgtt gtttttactt tgctattctt attacttgat
     9661 gcaggacagc tttttcacaa cctctcaata aacggtgttc aatcagctga ccctgggtat
     9721 tagctcaaaa cgctggattc acaacctctt ttatttcaaa taaccagtaa atacaatgaa
     9781 catctacaaa aaacttctat ttatttatag atttcgtcag gcaaatccaa ttctctctta
     9841 tcaatcaaag tctgatacca cgaagcagat tttttcaatg ttctgcttcg ttcatcgttc
     9901 aattcgattc gaactaatcc atatcgattc ttaaatgcat tcattggtga cacacagtca
     9961 gtgaatgccc ataacatata gccttcgcaa tttgcgcctt cttctgttgc tttgatcaag
    10021 taatgcaaat gctgtccgat aaattcgata cggtaatcat cttgcacgat tcctttctca
    10081 tccatatact gttcttcata ttctcttccc ataccgtttt ctgttatcag ccaagggata
    10141 ttatgataat tttcttttag atacatggct atatcataca tgattttcgg atagatttcc
    10201 catcccctgg aattattcat tttcttccca ggtaaatcaa agacttcata atatcgttca
    10261 ggatggaaag gtgtctcttc attccattga taacgagggg cgcgaacgcg tttaggataa
    10321 tattgattga ttccaaggaa atctactgta tgatgctgaa tcgtttctaa atcctctgtt
    10381 gcaggatcga aataaatcga ttgtttttga cacaactcca ggaactcttc tgtgtagtat
    10441 ccttttacca tcggatcaaa aaatactcga ttataaaaca aatcatacat ataagcagct
    10501 ttttggtctt ctggagaatt ggatcgagca tagaccattt caggatttaa aatacagcca
    10561 atccttccac cataatttcc gctgcggtaa atctctactg ctttagccgt tgctaaaact
    10621 ttatgataat tccacatcat ccactttttt gtgttttgtt catgcggcca gcgaacagca
    10681 tccaaatagc aacgcgtttg gggaacgatt ggttcgttga atgtaaacca gttttttacc
    10741 ctatcaccgt aacgatcaaa ggcaatttga gcatacttag cataaaaatc aactactttt
    10801 cttgaagacc agccatcata tttttccaaa agaaaagctg ggacttcata atgttccaaa
    10861 caaatcatcg gttctacgtc tgcttcgatc aaagagtcga tcatttcact tatatgctga
    10921 gcatattctt catctactat acagttctca taatcagtaa aaaaacgagc ccaattgata
    10981 gaagtccgat aatgtgtcag atggatggat ttcatcagat ccacatcctg ctggtattgt
    11041 tccataaaat tagttgctac ggctgggcca tacccattat gccaaacaaa atgttcattt
    11101 ttataccagc tatctataaa cgaatcctgt ccttcttttt ttcctttcca accttccgtt
    11161 tgccaagcag aagatgcagc accaagaata aaattttttg gaatagtaat tttcatgatt
    11221 tcctcctgtt ttttagatcc catcattttc cacacgattt gctactttta caaatggcag
    11281 atagatcaaa atagaaacaa taatacaaac aagttgcgta ataactgcac cgatatttcc
    11341 tgctgttgcc agataagcat tgacgatcgg cggcattggc caagggacca ttacaactgc
    11401 tttcgccgca aatcctaaag cggttgcaaa ataaccaatc gttccagtga taagaggcgt
    11461 taagataaac ggaatagcta aaattggatt cagcatgatc ggcataccaa aaataactgg
    11521 ttcattgatg ttgaagatcc caggagcaag ggataacttg gtgatttgtt tcatatcttc
    11581 ccgttttcca actaacaata ctgcaatcag taaaccgatc gtgactccag acccgcccat
    11641 actcatatac atatcccaaa atggcatagt aataatgttc ggtacttctt ttccagcttc
    11701 aaaagcttcc gtatttacag caattgccgc taaaagcaac ggttcccgta tcggcttcac
    11761 catttgattt ccatggatac caatcaccca aaagacttgc gcaataaaca tcaatagcaa
    11821 aatacccggt aacccttgta cgacaccttc taatgggcgt tgtacgacat tgtaaatgat
    11881 atcataaagg tacattcctg taagttcttt gatgataaat cccgcagccg cgatacccga
    11941 tacagtcaga atcgtcggga ataaagcaga aaaactagca gaaacattcg ttggcacggt
    12001 atcaggcatt ctcagctgta gtttcttctg tttcctcaac cagcaataaa gttcaataga
    12061 aaggatggag accaacattc ccaaaaataa ccctttcgta tctgtatatt gtttagccaa
    12121 tacgttttct actataacag tctgattttc attcactaat tcataaacag ttggattaac
    12181 agataaataa gacatcactg cgatgatacc agggaataca ccatccactt tattcagctt
    12241 ggctaattcg atccctatca gaaaaacagc atagatggtc aagaaactta gtgtgacata
    12301 gctgatggca gatgcgatag gctttaattc ttctaaaaaa cgcaagaaac ttatctgtgc
    12361 taagccattt tccgagtcaa agaccattgc tgaaaacaat gtgccaaaag cacccgtaat
    12421 aattaccgga atcaatgcag caaatgcgtt ttttatcacc atgatatacc ttaaattatt
    12481 gattctcgtt gccacgttcc ctaaaacatt ggctaattta tccataaaac tcattccaga
    12541 tcccctcatt cctgcttctt ctccttgacg tcatctcttt ttgtctatta gtcagcgctt
    12601 acatttcgat gaacagaaac tatttactcc tcttttttac caaatataaa acaaaaattc
    12661 atctgatgta tttatcataa aatacatcag atgaactgtc aattagtttc tttttctcct
    12721 attattttct gtaattctct ccgatttatt gccatatgga cagtcattgc atcaaacgca
    12781 gcaagtgcat tccgcctttc gatggcctga acgatctctg tatgaatatc gatcgtctcg
    12841 attttcagat gctggatatc ataattttta ttaaacaaag caattgattc attaatgatt
    12901 gggataatat gcagcaaggc tacgtttccg ctagcttctg caatcatcgt atgaaaatcg
    12961 atgtctaatt gaacatgttc ttgtgtgcct tctcgaaaag aagtttcaat cttttgcgtt
    13021 aattttttca tttggacgat ttgattcttc gttgcatatt ccgctgccaa acctgccatc
    13081 ttaggctcca ataaatatct cagttcaaat aagtctcgta tcatccgatc ctgatcttca
    13141 atcaaagaaa aaccgagtgg gtcattggca atccctcgct tatcactgac aaatgtcccc
    13201 gctccttgcc gaacttcaag gatattcctg gaagctagag cccgcacagc ttctcgcacc
    13261 gtgcttcgcc ccacctctaa ataatcggac aattcatatt cattcggcag tttatctcct
    13321 acttgcaact gcatttcctt taaatatcta aggatcttgt cagtagttac ttctactagg
    13381 gttttatttt cttccaaacc acatacctcc ttttcatctg atgtattttt tcgtaattat
    13441 tataacacgt ttgaatcatc tgatgatttt ttgtatacaa aattgattaa tccgttcata
    13501 gacatacaaa cgaagaagat atttaccata aaaaagacta ggataaaatt cactcctagc
    13561 cttcgatcat ttttattttg aacgacaaaa aatatgatgt tcaataacag acgatcttca
    13621 ttcgattctt gatgtcatct tttgtttttt ttaatatatg gataaaaagt ttgattacat
    13681 cgtttctatt ctagaatttg gtcgattctc aattcttctt ctggtgagaa atctaaatta
    13741 tccatcattc gaacactttc ttgtaactga cttaaccgac ttgctccgac cagtacactt
    13801 gtaacagatt tttgtcgcaa attccatgca agggccattt gcgctaatga ttgtcctcgt
    13861 gatacggcga ttgtttgcag tgctttgatt ttttccagag ttgatccaac ttgttcttct
    13921 gaaaggaatg gtatttcctt acgatgtgct ctagaatctt caggaatacc atgtagataa
    13981 cgatttgtca gcaacccttg agcaagcgga ctgaacgtga ttgcccctag cccttcttct
    14041 tctaatactg gagatagatc gtcttctagt agtgcacgag aaaacatatt gtagcgcatc
    14101 tgatgaataa tgaacggtgc ttcctttctt tttaaaattt cagtcatttt ttttgtatct
    14161 tctccattat agttggatat accaatatat aatgcttttc cttgtcttac aagttgcatc
    14221 aaagcttctg ctgtttcttc caaaggtgta tcagggtctg ggcgatgatg atagaagatg
    14281 tcaacataat ctagccccat tctttggaga ctttggtcac aacttgaaat gatggattta
    14341 cgagagcccc attccccata tggcccttcc cacatatgat aacctgcttt gctagaaata
    14401 atcagttcat cacggtaggc ttgaaaatct tctctaaaaa tacgcccaaa gtttttctct
    14461 gcagctccag cagggcctcc ataattattc gcaaggtcga aatgggtaat tcccatgtcg
    14521 aatgcgcctc ttaaaatctc tctttgatta tggataggat cataatcacc aaaattttgc
    14581 cataatccga gagataaaat cggtaatttc aaacctgaat gaccggattt acgatattcc
    14641 attttttcat aacgtgcact atctgcaata tacaattttt ctcctcctca tcaaatcttt
    14701 ttaaaacgct ttcttttttc tatttttagt ttactacatt ttaaagaaaa ggagttaatc
    14761 aaaatgattt tttcgtatat ttttttgcca tttgtttttt actcgttgat agatcagaaa
    14821 ttgattacgt gcttgctgga tttttttata acgtttcggt tgttttttgt aaaattgctg
    14881 atgatagttt tctgctggcc aaaacgtacc ggctggttcg atttttgtga cgattggttg
    14941 gtggaaagtt cctgaactgt ctagtttctg ttttgattgt tccgctaatt ctttttgtcg
    15001 ttcgacttga taaaaaatca ctggacgata ttgcttccct ctatcttgaa attgtccaaa
    15061 tgtatctgtg ggatcagtaa tttgccaata gattgtcagc aactcgctat aacttatttc
    15121 tcttgtgtca taaatgatct ccacagcttc cacatgacct gtgtaaccac cacttacttg
    15181 atcataattt ggtttttcaa catgtccgcc cgtgtatcct gacaaaacag ataggatccc
    15241 ttttttcgat tcaaacggct cgaccataca ccagaaacat ccccctgcaa atactgcacg
    15301 ttcttgatag tcagcatctt gcatggcatg cttggaaaag tcatattttt gctccttatc
    15361 aaatccttcc gtaatcaata aataaaagtc cattacatca ggcgtcagat tcattcggat
    15421 agctaatgga cgtagtgttg cttctagttt tgataactgt tctttaacgg atatattttc
    15481 tcttatgtca ttctttgtct gaatcaatac ttttcgttcc caatctcgtg tattcggatt
    15541 caaaatcaga ttataaagtg tttgaatgaa tatttgttcc tcatccatag attctcctcc
    15601 tttccgaaga agacaagcta gtgactttag ctgtaattta tcataaagtg aggaaataag
    15661 cgattacttc atccccctac agatttttcg tataaaaaag agagaatcct tatcgacaga
    15721 ttctcctttt taatcaattt attttataaa tgaaaagtta atttaccttt tatggaaaac
    15781 tattttaaaa aataaacata aatatgcagt taatgtaaca agataagaaa taatagtcgt
    15841 tactgtataa cttgtgctta ctttaatatc agcaactcct ttgactccgc ttatttgtac
    15901 caatccatta gcattcacct gctcctttac aggttgatga tttatttcta cattcgttcc
    15961 taaataagca tatacaggaa gtgtgattac ttggctcttc tcattagtta ctctatatat
    16021 gatagaacta ccagttgttt tataaggaag gtttacccat tgactgccaa tctttgcttc
    16081 gtgttttatt atatgctcct tattctccaa tgaagcgata ggagaataat catataaaaa
    16141 ataatccatt cctttagtgg tatacgcctc tactgtttct ttgtttacaa caatatgtcc
    16201 cggctgagaa attatttttt gattgaaatt ttttttacta gaaacagtta aagtgattaa
    16261 taacaaaaca gtcataaata aataaaatag ttctcttgtc ttattgtatc ttctattttt
    16321 gaaaaatatc atcgttaata tcagagaaga gaatattatt tgcaagctta agatccgcca
    16381 aggaaattgt aacaatttgg ctggcgtatt ttgcagaaga taccatggca ccaaactagt
    16441 tgtagctagc caagtcaaac acgtaccata ataaatcttt ttttctatgt ttgttaattt
    16501 tttaaataaa atcaggggaa tacataaaga taacagacca actagtccca ttgagtaagt
    16561 agttaagtcg ttgttcatag cttcagtcag actatcgaac acattagatg catgagcata
    16621 taaatttgtc acgctaggtt tgttgatttt ttggtagagc atttgttcaa acatcggata
    16681 ccaaaaagca ctcgtcagaa ccaatgtcat tcctgccgca ctaaataatt ttttaaatat
    16741 agcaataaaa tctgcattct tgaaaccttt agaaaccaaa cgaataaaaa taaaaatgat
    16801 aattaatgct gtactcatta atgttgatag tacatggctg tacactagca aactcatact
    16861 caatgctagc tttacagacg gatacttttc ttcttttcca gagatgattt gataagcata
    16921 aaataataca gggataacaa gtgttattgc gatagcttcg gcaattgcgg aacgatagta
    16981 gatgtctact gtccgataag cagagaaaat atagagacaa ctaaataaaa atgccgcttt
    17041 tttttgtttt aaaaaccgct cgccataatg ataagctacg agtgttgtac acaagttcaa
    17101 taaccaaacg taaataatat agcttacaat caaatttttt gtcatccaat ataacattac
    17161 agctggaaaa aaagtcagaa agggataaaa ataatttaca ccatatccag tataattgaa
    17221 tgtctcataa ttaatcggac tggtaaaaat agtcgataac cccttgattc gagataagtg
    17281 aaaagaaaga tctccagtgg tataaatact ctgtgtattt ttgaaaaaga tgaaagaata
    17341 tatactacta actataatta atccactcaa tattactact tttttattta ttttcattct
    17401 tcttgtttat ctccttttta gtcacatatc gagaatcttt caacttatta ggtgcaaatc
    17461 gtttaaaact tgaatacacc actaaacaat aagtcagaat actgatccat aaagccatga
    17521 gagcagttgg agtatatttt gttgtaattt ctatcttatt ttctccagca tttacatcca
    17581 cattcactaa gccatgtttt ttataagagt catttacgac tttaccgttt acttttacct
    17641 gtgtacctaa ataagcatat actggtactg tcactttttg ctttttgttg cttgccactg
    17701 tgtaagtaat gttattatct gcagctttat acttcccttt ttcccacgat tctcctactt
    17761 ttacttcatg tttttctaag tgaggccttt cttttatcgc ttctgttggg gcgtaatcgt
    17821 ataatcctcc catatctgat gttgtataac gtgggagtgt ttcttcattg ataacaatat
    17881 gaccgtgttt tgattggata acttttgcgt aattttcttt tgctgctata gacgtgacga
    17941 gtagaaacac cacagtagcg ccaatgatta accatgaata ttttttattc atagtctttt
    18001 tcataaaaac aagcgttatg atcattgaag aaaaaaagat ttggatacct aagatacgcc
    18061 aaggaaactg gagtaagctt atcggtgttt tttgaaacaa aaaccatgga aataaattag
    18121 ttacgcatac ccatgtagaa actacgccaa catagatgat tttttctttt ttctccattt
    18181 ctttcagtat gaataaagga aaaatcaacg caaccaatcc taataacccc atagtgtatg
    18241 ttgtaatatc gttatttaac gcttgaatga tactatctga aatagaaagt gcttctagct
    18301 gcaaatctgt tttgaaggga ctgtgtattt cttgataatt catctgctgc aacataggat
    18361 accaaaaata gctagttaat gcacaagtca aaaaaacagc ctttactaaa ttcacgacca
    18421 caaattttat atcgtttttt atttgcttgc ttaaaaacaa acgaactata atcatacaag
    18481 caatcaatgc actacacaat agtgtagtta agacatgagt atacacaagt agacttatac
    18541 tgacagataa agcgatgact tttttttctc tattgaatat caatccataa gcataatata
    18601 aaacagctgg gataatcgta agagcgattg cttctgcaat tgcggatcga taatagatat
    18661 caactgttcg ataagttgca aaagtatata aacaactaaa taaaaaagca gcatctatct
    18721 ttttaaaaaa cttttctcca taataaaaac taatcagaat cgtacaaata tttaataacc
    18781 aaatatatag aacataactt aatattaagt tatttgaaat tccataaaaa acgactgcag
    18841 gaattattgt aagaaaagga taaaaatagt tcagtccatc tccataattg ttaaatgttg
    18901 tatagttgat tggcccttca aaaatagaag ataacccttt gattcgtgaa agatgaaaag
    18961 ataaatcacc tgttgtataa atttcttgag catttctcat aaaatttact gaatataaag
    19021 tactaagtaa gataagaact actaaaagaa atgctttttt acttattttc atagatttgt
    19081 tgttctcctt ttttaattct attttcagta tactagaatt gtcttttttt aataagtttt
    19141 gcgttaaatt tttcatagca ctagtatgat atactagaaa agaatcaatt tttattactt
    19201 ttaaatagga gaaaaaaatg aaaaaaaaca aaaaccaaat aatagatatc atttttatgt
    19261 tatttctaag ttttgcttac gtcatccctc tattcatgtc taagggaatc tatcatagtg
    19321 tcaatcagga tacttacttt catttatctc gaatcatagg actagataat gtatggagtt
    19381 ctccagtaaa cttcaataac ttcgatcatc atggaacaat gatgaatatt ttttatccat
    19441 ggcttactct ttatccagct tttttatttt ataaaatgat gggaaatctt gttttaagtt
    19501 ataatatcta ctattttttt atcacttttt taacaatggt cgtttcttat ttttctatga
    19561 aacaaataaa aaacaatcgt tatatttcgc tgttattttc gattatttat acattttccg
    19621 cctatcgtgc cattgacata ttccatcgtg cttcattggg agaagctgtc gctttaacat
    19681 ttttgccttt gatactaatg gggtgttacg aaatttatat tagagattac caaaaatggt
    19741 actggttgtc tattggcatg acgctagtag tgtatactca tttactttct gtcgctatgg
    19801 tttctgtatt tattggagga acgttgtttc tttcttttta tttttgggac caaaaaatcg
    19861 ctagactctt aagtctttta aaagctaccg tcctcacctt ttttttaagt gcgggattct
    19921 taattccttt tattcaacaa tctagagcac aagaattgaa agtcccactg ggaaaagaat
    19981 taagtggtat ggctcctagt gatatgctga cacatatttt aaataataac tataacaact
    20041 atactattgg attattctta tttctcggat tgattggtgc ttttattttt ataaaaaaat
    20101 taactgctga tgatttattt attttctttc ttggtgtatt tgttcttttt tgttcaacca
    20161 atttatttcc ttggcaactt tttaaccata caccagttaa gagtctacaa tttgtctggc
    20221 ggttaaatgg tttttcttct ttatttatag cctatacgat gagtatagtg atctattata
    20281 ttttttctac taaaaataat tcatggaaaa taatggtctt tacgtttctt gcactaatat
    20341 tgcatctctc aggagtaagc aattctatcc atgctaacaa agattcatta acacttatag
    20401 cacctgaaga cgctgttgct atagctgaaa attacaatca tactgattat gcaaacaaag
    20461 agtcaatcta tcatcctgat atgattaata atgatcaata tttactaggt aaccaggaaa
    20521 taaatccaac aactcatttt atgagtaaca aacttacaat tgaattagat aattcgaata
    20581 ataaaaatac tgtcttgacg acacctattt atcgttataa agggcaagtg gcttctatta
    20641 atggcaaact agtacaaaca aaattatcta agtttggcac aactgaatta actattcctc
    20701 caggtataaa taaagtcgtt attacttatc agtacacaaa actagcaatt gcttcaagat
    20761 atttatctat cgttactttg attttatttt tactttatcg atttactttt tctaaacaaa
    20821 aacaaccgag aagagaagta caacattctc actagatttt tatttaagaa agacttttta
    20881 gctgtacaca taaacaggct aaaaagtctt tttttatatt tctttaattt gcttcataca
    20941 ttatttaaca tcgatattta tactgtgaaa catgcgtttc atccattatc aaagtatttt
    21001 tatcgataat aaacttcatc caaaaacaga ccttgtgcag ggacggtttc tcctgcatgt
    21061 tgccgcactt tgctttcaaa tatttcatcg attgtttcta tcggcatggc acctgtgccg
    21121 atttctagta atgtccccat gataatccgc accattttat ataagaatcc gtctccgaca
    21181 aaagtaaaat gaagcatttc tccctttcgt tcgatagata attcatcaat cgtacgcact
    21241 gtggattttt ttgatttttt caatgcagaa aaaccaatga aatcatgtgt acccactaat
    21301 ttttcgcaag ctgcattcag cttgtccata tccaactctt gagggaaata aaagctgtgg
    21361 tttcgctcaa atgctgaagg aatcacgttg ttccaaacat aatagctgta ttttttgccc
    21421 accgcattat atcttgaatg gaagcgctca ggcatttctt cgattctttt gatgacaata
    21481 tcccgcggaa ggtaacgatt cataaagtcg atcatttctg ccagtccaat ttctgtgttt
    21541 gttttaaaat ttgctacttg tcctcgcgca tgggtgccag catctgttct tcctgatcca
    21601 ataatttcta tctcttcatt tgtcatctga tgcaatactt tttcgatttt tccttgaatg
    21661 gttttatcgt catctccaag tctttgccat cctgaatatc tttttccgtc atattcgatt
    21721 gtcaacttga tgtttctcat attttagtgg ctcctctgtc ttccatttgg tcttctctct
    21781 acctttttaa gattgtagca caaaatatag ctccttgctt ctctaagaca gaagctatac
    21841 aagaaaactg tggaaatata tatttcagaa aaacaaaaaa cagccaccgg atgaagtatc
    21901 cgctgactgt tcaatttgac ggctcaagtt ttactgtttg agatgcaaga tactcaacct
    21961 ggcaatgggc acataattaa agaaaaaact gattctttgg caaaaaccta ttttattcag
    22021 taatggccaa gaatttatag aaggaattct tgccagtcca taaatcgccg caaaaagcaa
    22081 actgatcaca gacagtccaa ggtataagtg gcttacgtcc atttgcccat tttttcgata
    22141 gatcaagaaa agaagaactg gaaacaataa aaaaccactg taacctaaac ctgatccgat
    22201 attatggacc catgtcgaga cattccacga agattcatgg gtattgatac tgaacaaacc
    22261 tgtaaagata caatcaccta taccatagaa cgcgatggct gctgtaacta gggtagctaa
    22321 cacacgagat gtactaacaa atgcagcata caatgcaggc aatgacagga tgaaaaagat
    22381 ccctgaaacc acagaccaaa taagaaacgc ttttcttaca ggactggaaa cctctccaaa
    22441 tacactgatc acgctcttca tttgatctaa ttcaggataa tacaatccta atacgtacgg
    22501 agtcaaaaaa tcactcagca cgcctaataa taaaaagaaa aaaccaaatt tacgtaaaaa
    22561 atccatgata cgcctccaat aacaattacc tatgctttta attgttatta taaacgactt
    22621 tatcttgatt taacagaaac ctctatttac acgaacatct atataactca ttaaaaactc
    22681 gaatcatttt aaaaatacta aatatctaac gttcaacgtt cacttaatcc atctaaacaa
    22741 tctttcaatt tgattttttc cccacttctt ttacgcgcgc ctagctctcc tattgcagta
    22801 aacaacagat cacttgacga atttaaggca gtttccattg aatcctggat aacaccgata
    22861 ataaatccaa taccgacgat ttgcatcgcg acatcattag agataccaaa caaactacat
    22921 gctaatggaa tcaataacag tgaaccgccc gcaacacctg aagctccaca agcagctaaa
    22981 gcagacaaaa tacataaaag gactttcaaa acaaaaggtg cttcaatccc taaagaatgt
    23041 acagcagtca acgtcatcaa ggaaatggta atcgcggcac cgcccatatt gatcgtagca
    23101 cctaacggga tcgatatgga ataagactct tttgtcaatt tcaatctttc agccaacatc
    23161 atgtttatcg ggatatttgc ggcagaactt cgggtaaaga aagcgggaag tgcgctttct
    23221 ttcaaacaaa agaaaatcaa tggataggga ttttctttaa taaacaaaaa cgtgaggaat
    23281 gggtacacaa ctaatgcgac aaacagcatc gtaccgatca aaacaagtaa aagctgcaga
    23341 tactctgcca atccagcgat tccagtcgta gcaattgatt gataaaccaa tcctaaaatc
    23401 cctatcggag caaatgcaat aatcacttgt acgacttgtg acaacgctat agatatctga
    23461 tccaccaact cttttgtcga ttctgaacgt gctcgaaaag ctagtcccaa caatacagcc
    23521 cagaataaga cgccaaggta attcgcatca ataagagatt gaacaggatt ttgtgtgaca
    23581 ttcgtcaaca tagtagataa aacactttcc aaactttgcg gtgctttttc agatactgtt
    23641 tcttgtaaag taataggaac agtaaaaatt gaacttacaa taactgctac aagtgcagaa
    23701 agcaatgttc ccactaaata taagatcaaa ataggcttca cgaatgtctt attcccaatt
    23761 ttatgcttgg caattgacgc catcgtcaag aaaaaaacca gaataggtgc gattgccttc
    23821 aagcaactga caaacaacgt tcccaatatt gaaataaatg tccatgtggg gaaaaagata
    23881 ccaaacaata ttccgactaa aataccaaca acaatacgtt gaactaacga catatccaaa
    23941 aacttctcta acaaatgacc accctttcac aattttaaca atcattgtat cattttatgc
    24001 aaagaaacta aaggtataat tttctaaaaa ttctaacaat acaaacaacc atttcttgct
    24061 aattttgtac tttttctgtc tacacgttgt tttttgagat attctaagtt aggatgttat
    24121 taatgggcaa cttatcagga aacgttttct tatgtttttg tccaattttt ttgtgaggtg
    24181 tttattcttg aaaaaaataa agaaggagag ttatggcttc ctgacaatct ttgtcgcttg
    24241 tataatgatg gttttacagc gtatattgat ttcagcagtc actattccta catatgcaac
    24301 aaaccctggt cgattctaca tctatacatt tttacaaact tccgttgtta ttggtgcaaa
    24361 tttgatccca ctttacatgg gttaccaata tcaaaaaatc gcccgtttaa aagttttaca
    24421 ttatttaagt cgttttgcct ttatattcct aatagccaca ctagtttgca atatcttttt
    24481 ttacgtacaa gctggcagct tgaatattcg tgattattgg cttatcctaa cacctatcag
    24541 tcagaattac tttacttatg ctgtttcttg tactttgctt ttttgtagta ttccttatac
    24601 agttggattt ttaaataaat tatcgaaaac aacaattaaa tatctactat tatttttaac
    24661 aattggatta gttggctgct ctactctatt taataaagac ccgtttgcat tacagaatgg
    24721 acagagtatt ttatggattt tttatttatt tttaatgggt tatggtttga aagaatggaa
    24781 ctggaaagaa aaggtccgct ataagcttcc tcaagtattt atcagcttga ttgttttatt
    24841 cggtttgatt attttgatga cacaaatttc actcatcatc agaggaaata cagatacagc
    24901 gctgaggttt tctaaaccat attctctctt cgcaatgtat tatactttct cttcgttttt
    24961 atttttagaa atgctatctg ataaattagg tataaaagga agaggaaatt ttcttgctgt
    25021 atttttgatt gtcactcaag ttatatgtaa tttcccttta gtttcttatc gcgtatctac
    25081 atttatgaaa cgtgaatttc caaactcagg gaaggcatgg ctgataaata tcgggcagtt
    25141 ttttatttga tatttattag cagtgtccct cctagttatc tttttatttt atatacaaaa
    25201 aatccgtttc attcatcgtt tcatcgaata cttcgctttt gattcgattg aacaattaac
    25261 tcagcgttgg aaaaaaagaa ttcattggct ttttgttcat aaaagttatt ttttagttgc
    25321 agcatttttt tactgcttta ccttcgtgca gattttcgtt ttagaaccaa aagaaggttg
    25381 gaaagaaact atccaggttg ccttaaaaat atttactcag cgacaagcac ccatgatcct
    25441 gacaatcatc attattatgg gatttttctt cttactattg ttgatttcca atcgtttttg
    25501 gtacgccttg actgctacct tgatcattaa cttactgctc actatttcga cagtaatcaa
    25561 aatggaaatg cgggaagagc ctgtctttcc ttcagattta aaaatgctga ccggattgtc
    25621 agaactatta tcgatggtta gcccagtctt attaatcgtg ggcggattga tccttctcct
    25681 attattgatt actagtatta ttgtacaacg tagactacaa aaacagtatt ccttgaagat
    25741 tcattggaaa cgtcgattta tcagtatcgc tgtattatta ggatgttttt cgggtgtctt
    25801 tttcattaat cacaaaaatt cgccttcttt cttgctgttt aatttattta aagtaaacaa
    25861 gactttcttt aaccagcaag gcgccgtaaa agataacgga ccagtcattc aattcctaaa
    25921 caatatcgac attaaaatca tggaagaacc tgctggctat tcgaaagaaa aaattaatgg
    25981 aataatgaaa aagtacaaca caaaggcaga tgaaatcaac caaacaagaa gtgactggtt
    26041 aaacaatcag acgatcgttc tcaatttaag tgaaagcttc agtgatcctg cacgtgtccc
    26101 taatctgaca gtacctacag accctattcc ttacatcaaa agcattataa cagaaggaac
    26161 aggaggattg atgttatccg tcggttatgg tggcggaaca gctaacatgg aatgggaagg
    26221 actgacaggc ttgagtatca gtaacctgtc tgcttcgtta gttacacctt atacccagct
    26281 agtggagcgt cagaaaataa gtccaaatat taccgatcta ttcgatgaaa aaatagccat
    26341 ccatcctttt acagctgctc tttatcgcag aaaacaagtg tttgataaat ttggttttga
    26401 caggttctat tatttagaca gtccggataa attaacttat acggataaaa tagaacaaag
    26461 cccacgcata tcggatgatt cagcatacaa agaaaccttg aaagctttac aaagcaatac
    26521 ggaaacgtca caattcatcc aattatccac tatgcaaaac catatgcctt atgatgatta
    26581 ctatagtgaa ttgaactaca ctgctgaagg aactgctgtc attcctaata gaagaaaaga
    26641 attacaaact tatatgcaag gccttcacta taccgataca gcagtaaaaa aattcatcca
    26701 agaaatcgac aaaatcgata aaccgattac ctttgttttt tatggtgacc atttacccgc
    26761 aatctatagt gggaatgatt cgaaaaaata tggattagaa cagcaccaga cagattattt
    26821 catttataac aacaagtata gtcgtgaaca agcagctacg ttaaataaag aagttgttgc
    26881 tcctaataac tttggtgcta tggctcttga gcatgcgaat atcaaagtaa ctccttacta
    26941 tgcgttgttg acagaagtat ctaatcatct acctgcatta acaattgatc ctacagaaag
    27001 cttgtctaat cgttttaatg gaaaacaggt ctttgttaca gaggatagtc aaattatcca
    27061 agaaaaaaat ttaactgaga aacaaaaaga aattctagaa gattatcgtc tgatacaata
    27121 tgatcttgtt gcaggtaacc agtacagtgc aacatgggcg acacaaaaaa taaaagacta
    27181 acaaataaac tgagcaaggg gttgtgataa aagacatgtc acaacccctt gtttatctca
    27241 ttaatttgct tgtccataaa cgatttgatt aaagccattt ttcaattcat tattcgctgt
    27301 ctcgttgaag taaagataat tatcgggatt cccctgattt tgttgtgtca ccaatacttt
    27361 tggctgattt tgctgaaacc cgaatagata gacgtgcttt ttcagatatg gttctgtctc
    27421 tgcatcggaa tagacggctg tgatttgata gtctgcttga cctgtcccac tttctgacca
    27481 ttctgcagaa acaggcactc caccaatagc catcttccac tccccgttca aaatagcttg
    27541 agggacttta agaccatata agtccacact catttgattc gtataacttt tgtattgttg
    27601 tcccattgtt tggccccatt tggtcataaa tgcctctaat tgtgcggatt tgtctgcatt
    27661 ccacagtgtt tgttcaactt gttcgctgct ttctgaagag gaagatgaag cctctacaga
    27721 agaacttgaa gtagatgttt gagttgtttc agtcgttttg gaagactctg acgaagtaga
    27781 ggtatctgct gtttctgtac ttgctgcagt tgacttgctc tctgtgctag ttgttgtagt
    27841 agttgcaact gttgctttcg ctgtttcttt tgttgattct tttttcgatt cattcccgtt
    27901 ggtacaacca gctaacaaaa aagctagacc aattgttact aaaatccttt ttttcaatta
    27961 aaaaactcct ttcgattact cccacttctt tattctatca aataagcaat gtgattttat
    28021 tgatttttcg tattttctca tcaatctttc aaatcttcta aaaactgaga agcacccgct
    28081 gatgttgtct gtaatacatc agcgggtgct tcttttattt taatcttcgt gattttccaa
    28141 tttacctcac gctttatatt tgtatttgaa gaaacataga cttttagtca tcatcgtctt
    28201 cctcatccaa atcttgtcgg acagtttttc cttggagtct ttgtacttgg tagatgattt
    28261 ctttattgaa acttaaaagt tcaacaagca attgttgcaa gccatatttt tcttcttttt
    28321 cagctaattt gattgcccgt gtgataaaga cattttggaa atcaaaatct tttgcaaagt
    28381 tttccaacat tgttgaggct ggtaaatatt tgttttgacc atgttcgtaa atttttccat
    28441 aacgcatgac ttcttctaaa gtagacggaa cgatttcatt ttcaatcact aactcatgag
    28501 agattttatt gaaccatctc gcttcttgat cagcatattc tgaaaaaagg gtacttaatt
    28561 cttgcgaaga tgtacccaaa acatacaatt gtgcttggat caatttcatt tcttctaatt
    28621 taaaatttgc taatacatgc cccaccatgg cgccagcagt tggtttatga tggtcgatat
    28681 ctgcttgttt tgtttcttga acaaattttt cttccactat attcattgat ttacgcctct
    28741 tttacttttg ctgattggac ttcataacct aatttttcca caactgcagc taattgttca
    28801 gggtggactt catctggatc aaagtttgct ttgattttgc tcgcattaaa caaaactttc
    28861 gtattgctta ccccaggctg ttgagaaacc gcatgttcga ttttttgcat acagcttgga
    28921 caagctagtg tatctaattg cataataatt tttttcatcg tagttcttcc ttcctttttc
    28981 ttgatagtct tattctagat tacagcttcc ataaaaaaat tgattcttat caagttttac
    29041 gatttttctt ctttgctttc gtgtccaaaa tacaataaac gcatcgcgtt aaagataacg
    29101 atcaagatac tagcttcgtg aacgaacatc ccactagcca tgtaaataaa cccggcaaat
    29161 aagccgttga gtaaaaataa gacgacacca atggcaatca ctacattttc acgggtattt
    29221 aaaacagttt tcttagccaa gcggtaggca tgaaccaacg attcaaaatt agattgcatc
    29281 aagacaacgt cactcgtttc gatagcaaca tccgttcctg atcccatagc aatcccgata
    29341 tcagccgcag ccaaagaggg agaatcattg attccatctc caataaaagc aacgcgcaat
    29401 ccttgttctt ggtatttttt cacaaattga accttttcgt ctggtaataa ttcagcatgg
    29461 acttcatcaa tgcctaacat atcagctaca taatcagcag tcatctgatt atcacctgtc
    29521 agcatcacta actgttttgc tcctgcttgt ttcaacttag ctaattgttc agcgacttct
    29581 ggtcgaatca catctgacac acctatgatt tgagtgattt ttttgtcgat tgctacaata
    29641 atgaccgagc tgcctgtttg ttgtaattga tgtaaatctt gtacttgctt tggtgtgaga
    29701 gaaagtttgt gagcggcaat caatttggtg ttgcccgcac aaactgtatg accatcaatc
    29761 tcagctgtga ttccttgtcc cttgattgtc tgattatctt tgactgctaa ttgttgaaaa
    29821 tcaatgtctt tctcctgagc ataatggatc acggcgcgcc ccagaggatg atcggataac
    29881 gtttctaatc gagccactaa agctaagctg tccccttcat cctctgtata gttcttgata
    29941 gtggatacag cagtagttcc tttggtcaat gtaccagttt tatcaaagac aaaagtatcg
    30001 attttggaaa aagtatccat tgcttcaccg cctttgacta agatgccccg tttcgctcca
    30061 ttaccgattc ctgctacatt cgaaacaggc gcaccaatga ctaaagcgcc aggacaacca
    30121 agaaccaaaa cagtgattgc taaacgaaaa tcacgactaa tcagaccaac aaccaacgca
    30181 ataagtaaaa cggttggagt ataatactga gaaaatcgat caatgaattt ttccgcatgg
    30241 gatttggtat cttgtgcttc ttctactaat tcgataattt tggcaaaggt cgtatcatct
    30301 ccgaccttag ttgcttcaat cgtcacgtag ccatctgcta aaatcgttcc agagaagact
    30361 tcatctccac tgattttgac gacttgtcgt gattcaccag tcactgcggc ttcatccgca
    30421 taccctttcc cgtcaatgac tcgtccgtcc acaggaatag ctccaccagt ttttactaag
    30481 acgatatcgc cttcttccac gtcatcgatt tccacttctt cagtcgttcc atcttcttgg
    30541 acaacactag cagtagatgg ggccatctcg gtcaaagcct tgattgactc ccgcgttttg
    30601 gtcaatgttt tttgttccag atagctacca aacaaaaaca agaatgtaac gatagctgac
    30661 tccttgtatt ctccgatcat aaaggcacca agcacagcaa tagtaacgag taaatcaatt
    30721 gagataactt tgactttcac tgcctgatac gcatggatca taataggtat gaccgcaata
    30781 atagaagcta tggctagagc aatgttatag aacaagacaa aaccagtaaa ttttccgata
    30841 aaaccaaaaa taatcaacac agctgataag gcggtgatat gatttcgatg ctcttggatc
    30901 cacttttgta atttcatctt tctccctctt ttcctttcat tcttacgaga ctaagtatag
    30961 atacttatcc atcataaaaa attgaccact atcaagttta gtcaaaaagg cggttttctt
    31021 ttggtagaat acactacaaa aaatagttag caagaagact cactaaaagt gatcttgcca
    31081 actattgtcg tttctatcga aattttcatt gaatgatttt cctgtacagc atacatcgga
    31141 gatctatttc tttatctaag atttttatct tgctaatttt ttcctttagt ctttcaaatc
    31201 cacgtcgttc atagaattga tacgtacagc cactatctgt aaaaaggaat atttcttttc
    31261 ctttctctct tttttcaaat tctgttagca acttggtacc gatgcctttt gttttcaaat
    31321 caggatttgc agctaaaaaa atgatctctc catctggcga atgcttcttt aaatatgctg
    31381 caagcatttg ttcatttgct gtctcgtaag ctcctttact ttctttagca aatatatttt
    31441 gtaaaaaatc gaatatctta acatatattt gctgtccaaa agaacgatgt ttcttttttt
    31501 ctccttttat ctccgccagc aaaacacctg ccaactcgtc ctctgcatac aaagcaagaa
    31561 tttgagttgc tcgattcaac tccatataca aaaaatatct tccgtaaaga ttcagcagaa
    31621 gttcattttc catatatctc tcaaagttca ttccttcaac agcaaaatag atagcttttt
    31681 tgtaatcttt cttttctaag ggtttaaaag ttagtttcat catttcgcct cctcaacttc
    31741 aatgattgtg ttatacttac attataatag acacagtgtc tactatctat aatcaatcat
    31801 ctataagttt gtcgattaag cgacagcttt gctttgatat gtcggataag gagataggta
    31861 aatgaaaaag acagatagac gcgtcaaaaa aacagaaaaa gctttggctg aaacactatc
    31921 cacattatta gtaaataaaa agattcaagc aatcactata cgcgaactaa cagaaacggc
    31981 agatgtacat cgttcgactt tttatacaca ttataaggat atctatgatc tatatgatca
    32041 attagaaagc aacttttttc gggatctaaa tgaaattctc tcttatgatc cagcccatag
    32101 ctatgaggaa ctatacactc gcttgattga ctatgtttac gcaaatactt ttttatatcg
    32161 cttatttatc ctcaaccatg gtgatatttc gtttcagaag aaagttaaac aagttattga
    32221 agaaaactat ctgaacatat ggctatatga agacaagaaa acgattatca cagaagaaat
    32281 gagattcttt actacgtacc acgtccaagg ttgtctatcc gttctcaatc ggtgggtaga
    32341 aagcaattac aagtactcca aaaaagaatt attagaactc ttacagaaac tgaatgatca
    32401 catagagaaa atcatgcctt gaataaaaac atactaagct tagttaagaa gaaccgtaac
    32461 cttctcgtat aacaactgaa tcttatttag ccaaattgtt tactgaagat tttttgagta
    32521 aaatttctat aattcctact tattgaaaag tagtttgtag tgctgcttct ccctctctaa
    32581 gtgatatatc ttgttgtacc aatcaaattc acctcatcat taataaatta cttttaggct
    32641 aatttattac aacttatagg ttgaagctgt aaccaattta tagtcatatt atatttttat
    32701 catccagttt attgaattct tctcgtttac ctcaattcat tttcaagggg aatcagctca
    32761 tcatctaaca ttttacgcca tgctcgcttc atttcttgtg ggtatttaaa tggtttcgct
    32821 tctggatgtt tggctttata tccttcgtaa tccttactgt tgataattgc tttcatatta
    32881 ggaattggat cttcacctgg catatattgc agattagaag catgtaattt tttctcagaa
    32941 acaaaccact cattggaaaa gacatgaatc aaatcatcac ttacttgtgt aaaatagcga
    33001 cgtttaatga caaagatatc ttcattgcta tctagtgtac cttcatttct ttgtgcttaa
    33061 attcaaagat aattttctca ataaagattt ttgattccag taagattcct ttactccttt
    33121 tacaacatag cgttgtcctt tgttttgaaa catcttcttc ccctctttgc cattaaaaat
    33181 agcccaattc tctataaaga agattaagct tcattattat aatataaagt ttaaatttgg
    33241 tttattactt actttttctt attctctagt aagctaatat tgaaaaaaat gtaatcagat
    33301 cattattttt atatgttgta acttcctgat aagaaaacta attaaaagaa agaggtaact
    33361 acataaacta attaaaaaag ctaacttcat agttggagga taaatggaag aaacttcaat
    33421 gattgtttct ccttgtgttc cttcaatcga cactagtcct attatcaatc tctctttttt
    33481 gtccatttac ttttgcgact gttccccaat aatcaaatac aggaagcgtt attttttgtc
    33541 ctttagattc atgtactgta tagactattt tcgaatccat cacttgataa ggcatagtat
    33601 gccattcctt gccaattctt acttcatgat tctgcaaatg ttctttgtat tgtaatgctt
    33661 ccgtgggtgc atagtcataa agtcctccgg gaaggagtgt cgtgtaacga ataagatttt
    33721 cttgattcat taccaagtga tctctctgag tgacaacttt atgttcatag tttgcttttg
    33781 ttgcagcaga tacagccaac agtaataaaa caattgccgt tatattaact acttttcttt
    33841 gcttagctga tgtgctcttc atgaaaacga acgataaaat caacgagcca aacagtactt
    33901 gaaagcctaa gattcgccaa ggaaattgaa tcatttgaat gggtgtattt tgtaataaag
    33961 accatggaaa aaatttggtg gataagagcc aagagactac cgcactaagg taaatcactt
    34021 tttctttgcg attaaatcct ttaaaaaaga agagtggaca gatcaaagaa ataataccaa
    34081 tgattcctac cgtaaaggtt gtcaggtcat tattcatcgc accaatcaaa ctatccgaga
    34141 catttaatgc ttggtgttgt aaggtcgttt tatctggctc attaatcgat tgaaccatga
    34201 tttgtcggaa catcggatac caaaagtatg ctgttaaaag aatcgtcatt cctactgcct
    34261 ttgacaactc cacaatcaac cggatgcttt ctttttttct tgtttcactt actacaaagc
    34321 cgcctaagac tagtaagcca acggctatta cactcataaa agtggaaaga acatgtgtat
    34381 aaatgagtaa gctcatactg actgctaatg gaattgcaca tggctttttc tcataaatca
    34441 gtgcgtaagc ataatacata actatcggta taaccgtaag tgcaatcgct tcagcaatag
    34501 cagaacgatg ataaatatca atcgtacggt acccataaaa tgtatataaa cagctaaata
    34561 taaaagccgc atcgattctt tttaaaaatt ttagtccata atgaaacatc agcatgatag
    34621 tacaaacatt caatagccaa acatataaga tataactaac gattaaatta ttacttatcc
    34681 aataaaaaat gactgccgga aaaaaggtca agtagggata aaaataattc actccgctcc
    34741 cataatgatt aaacgtcgta aaattgattg gaccagaaaa aattgaagat aaccctttga
    34801 ctctagcaaa atggaaggca atatcccccg atgtagaaat agtatgaaca tttctaaaat
    34861 aattgattga ataaagtcca gcaataattg ctaggagaat ggacaatatt atttttttct
    34921 ttacttgcat tacttaatta tatactcctt tcatggattg ctttatctag tataacttaa
    34981 attttaaata aatcgttatt tatttttact ctcacttaaa aaagcactaa aattataaga
    35041 gagggaggta atggtctgaa caccaagaat tgttccaaca tcgaattgct taactaatga
    35101 gttcaaagct taattatgtg gctcttatat ttaccgagcg tggattgcga tctctttagc
    35161 gaaacaaaca tctttcataa aaaaaacagc catatccgct ttcttggata tgactattta
    35221 ctactacttt actatctttc attcttcttg tctcacttat gataaggcgc tgcgtatcat
    35281 gagtgaatga tattaggaaa acttcataaa ataaatcttt tatacatttc cacttctaga
    35341 tggtcattca tgttagaaga atcaaactat ttttttactg tcgtaataat attaaaacac
    35401 tagaacaaat caattaaatt ccataaaata taaataatag acctagcatc atctggtata
    35461 aaatggatgc taggtccgtt atatttactc aaaattgatt ctacgaatag atcttcttat
    35521 catttctatt ttagtagtca tgcgttgttg ttcttctaaa ggtggtaatg gtataagtag
    35581 tttagataaa tttgtaccat ttacatttgg ttgcccagtt ccgtaagaaa ttttttctaa
    35641 taacttccaa tataatggcg agtctaagaa gcagtctacg tattctgata ataatttctc
    35701 aactaaccta attcgaatca agtaagaagc aaaaacagat tcctcactta tttccttaac
    35761 caagaaactt tttcccatag ttcctcctgt ccttgctatc aaaatgtcat tttcttccaa
    35821 tcttaaatca actagtttag aattactaat atcacagtaa ggcacactgg accaatttac
    35881 acgtccctct tgtatatctg tgattctcaa aaacttaaca ttgccttgtt ttttcgccga
    35941 atctgtatag ccatattgaa tcttagtaga aatacttcct aaataaaccc atagtcgaac
    36001 atcgctagat gatttttctt gtcgttcttt atgaacaaca gtgatcatct tgagttgtcc
    36061 gactattttt tagttgcagc aatctttttg ggacgcttat ttgcatgaag caacgttgct
    36121 aagtctagta ataaaacatt ggctaacaca ggattggctt tctcgcgata cgctgcggcg
    36181 ccactttcaa acaatactgc tagacgatct tctacaacaa cgatttgttc caaatacggt
    36241 ggaaatttga ttcgttttag gatccggcta ggagagtccc gctcggacac taagagttcc
    36301 gaggtataag gaccataaga acgactggct aagagataat ggccataaaa agccacacct
    36361 tgaatcttat cagatgtttt tctgctcatt gtcacggttg gttggatttt gtctttgcgg
    36421 cgaattgctg ttttcatttc agctatcgtc ggcaatagat agaaagtcaa ctgtccttca
    36481 ccttttagtg tgaagttgcc agataccagt tgatcattgt tttgagtcaa atacgatgct
    36541 tgtggaatct ctgagagatc agttgtcagc caataagcga ttggctgtcc cgtctcttca
    36601 gatgtatctg ctaagatatc gatcaaacgt aaagcggcta ctcgtccagt agcagatcct
    36661 gtgattgtca accataaaag ttcccgtttt gtatcatata ctaagccccc tacatgtggc
    36721 cgtttaggta aaatcagcag tttgatcggc tgtcctgttt ctctatccag tacatgaata
    36781 atagaatgat gctgatggtc atagcagtag gcagaaataa acacaaaatc ttgagaaaaa
    36841 gccaaccctt gcggcgtcat ccattgacaa acctcatttt cgccggtttc cggcaccacc
    36901 attcttgttt ctgttaatcc aggaatgtta tattgttttg cttcagcctt ttcccaagct
    36961 gcttgaacaa taggataccg ttttaacttt ttttcaatat cctgatgtat ccgttgctgc
    37021 ttccaatgct taaactttag gacatagtaa acaagaaggc caatgaccag caacactaaa
    37081 atcattattg ctacccgctg tgtcattttt ttgcctcctt gttctattcg atcaatcgtc
    37141 catataccgc tattaattac cgtaactaca tgtttctttt actagcgttt ctttatagga
    37201 tctttcataa tctcatttat agtaacatta agaatccctt attctactat agtcacttcc
    37261 acatcaatat tgcctcttgt tgctttagag tatgggcaaa atgcgtgggc ttcttctgcg
    37321 attttctctg cttggtcctt ctcaagtcct ggcaaagaaa cttctaaaac gaccccgatt
    37381 tggtagcccc catcttctaa ttgaaataaa gatacccgtg ctttgacttc agaatcagac
    37441 tcaagattat tttcttccag catgtgttga accgcgccgt tgaagcaaga tgcgtatcca
    37501 gccgcaaaaa gttgttccgg atttgtccct tcactatgga ttcctgggcg gtcgatcttc
    37561 atgtgcatac ttccgtttgg tgcctcaact tcgccatcac ggcctccacg attgatcatt
    37621 gctgtttcgt acattttttt cattgagatc atcctttctt tcgtcgttct atattaagtg
    37681 taacgagtat tttttaatct gacaaaggta atgcttgcat tcggcgacat cagttagaca
    37741 acatcgatga tcagtgatag aagcagttta tctatacaaa aaaagaaggg ttctatgata
    37801 tttggtgtcc ttactcgcct tgagtaacaa caccaaatat catagaacca cttaatcagc
    37861 ttcacactgc tttttctact ttcgtagttg aaaaaaactt aaacaaatca tcggcaaata
    37921 gttaaagaaa aaacttaatc gttgaaagaa gcccaattgc ttaaagaagg caaactgact
    37981 aatcgacgga atccgtgcta atccgtaaag acctgcaatt agtaaactaa taactgttaa
    38041 aacaagataa aaatggctaa gcgtaccttg accactttga cgataaagca gaacaagtaa
    38101 caatgggaaa agtagaaagc ctgcatagcc tagcccagaa cctatattat ggatccaagt
    38161 cgatagatta caagagcttt cattcgtatt gatactgaat aagccagtaa aaatacaatc
    38221 acctatccca tataaaccaa ctgttgcaac aacaagtatt gcaagtgttt ttgatgttcc
    38281 aacaaatagg tgatacaacg caggcaacga aagaacaaaa aaaagtccag atacaactga
    38341 ccagaccaag aaagcacgtc tgactggact atctacgtca ccaaacacac taattacggt
    38401 agtcatttga ttcagtttgg gataaaaaag gccaaggata tacggtgtcg ataaatcact
    38461 catcacacct aataataaaa aataaaaacc atatttttgc aaaaaaatca ttttcttccc
    38521 ctacttttta gtgattattc gatacatcca acaatcaaat tttttctcat tttaacacat
    38581 atttttattc tagttgtctt ttgaacaatc aataacataa aggtgagttt cttctacatt
    38641 ttaaaaaatc gaatggcact gaagagttca gttgtcagca tcaatctcct actcaaaaag
    38701 gcaaaaaaac gtaagtcaga tgttgttgac atctcactta cgcatccatt gattgaaaat
    38761 ttcttagtaa aatctactag cctaaaaatt tgaagacttc ttctcgatca tacagtttaa
    38821 atactttctc gcataaaaat tgagtaatta gtgccgcaaa aacagggatg actaaccaac
    38881 aaagaacaac aagaaagcca tttagccctg catctaaaga agccaacgga ccaacaagtc
    38941 cgaccacgcc gaaaccagca gaagcaggcg tacctgaaat agaaaataga gcgacaggaa
    39001 tagctgtaat cactgcacta actaaacaag gaataagaat cacaggtttt ctaaatagat
    39061 taggcatcat catcttcata gcgcctaaag caatcgcaat tgtcacacct gacttattaa
    39121 ccttccaaga gttcactact aaaacaaaag tggttgcagc tacgcccatc gcagccgcac
    39181 cagcagaaac accatctagt tggattgcca ttccgattgc tactgttgaa ataggagaga
    39241 taatcaaaaa ggcaaaggaa caaccaatca agatagacat taacagcggt tgtaaggtag
    39301 tgaagttatt aatcaatgat ccaattgcag aagtaattgc tgttacaaaa gggaataaat
    39361 aaaaaccaag taaacctgca cctacaccta caacaatcgg tgttaacaca atcgacactg
    39421 acccaaaact atttccaatc agtaaaatca gcccgacagc aatcgatgct gtcagcatcg
    39481 tattaataat atcgcctgta ccagcaccaa cgtaaacacc cgtttcaccc aaatcaggtg
    39541 tgaatttgac tacaccagaa ccaacaaaag aagctccggc tacaaccatc attctcatcg
    39601 gatcaaatcc aaattgtaag gcaatcaaac caccaatgat caaaggtgtt gctagttgaa
    39661 aaataactgc taactgtgcg atcgttaccg caaagggata cgcactaaaa tattttaaaa
    39721 tggcacctaa tactgcatta ggaatcaatc caataatgat tcctgttgct gtaccagcga
    39781 ggactttatt cagaaaaatt ttcggtgata ttttttgtga agtttccatt caatctctcc
    39841 tttattttac gttcaaacta tcttcttttg catggacaag ctcagtcaag aaatcacaat
    39901 aaggcgtcgc aacaccatat ttcttccctt ttctagaaat tgcgccattg atataatcga
    39961 tttctgttaa tcgatggttc ttaatcaagt cttgatacat tgaaggataa tgtaacccaa
    40021 ttgtttccgg gtcaaaacaa gattcacaat gtgcaatgac ttctgggaca tctagttcaa
    40081 tcttctctac tgctgctact ttggcaaact cattgacaat cgtcgccacc attttatgag
    40141 cagtcgatgt ttttcctagc tcagccatat taacgtctaa gattgtgcat agtccgttca
    40201 ttgttccgtt cacacatgct ttgcgataaa tagaatagtg aatattgtca gaaaaatgag
    40261 catttaatcc tgattcagac aatttgtctg ctaatttttt tgcagctgct tcttttccat
    40321 cacctaaatt ttgtaattct actgaaccac ttccaaataa tttgacctga ccaggacctt
    40381 caagacctgc cgtccacatg gtatttccaa tatagatatt ttccattggt acaaatttct
    40441 caataatatc ttcatgcccg ataccattta atagacataa gacctctgta tcttttttga
    40501 ttaatgattg gatatcctgc agcatttttt ccagctgcat cgctttggta aatagaataa
    40561 tcagatcaac ttgatcttct ttttctactt cggattgaag aacgattggt agttttgctt
    40621 ctacctcttt accattaaaa tttgcttgta atccatgttc tttgatttgt tggacatgtt
    40681 ctgcccagcc atcgattaat aatacttcgt tcccactttg gtgaagcatc aacccaaagc
    40741 gacttcccat tgcgccagcg cctgcaattg ctattttcat tgattcttcc tcctttttct
    40801 ttctattata ctcatttgag aatcttttca caacctcgtt ttgttattct tctcttattt
    40861 atcatgttta tatccaccat tcaagttatt ttatcagcaa atcaataaaa actggaatag
    40921 aatcccaatt tcatcgtaag agaaactaga ctagcaataa gaaaatagaa acattcatag
    40981 aaaaaatctc actcctctga ttataaaaga gggggcatta acctcgtgta tactaaaacc
    41041 ttccattcat tcgatggatg attttctttt tgtgaaaaaa tattcattgt agtacgatgt
    41101 tgcgcaacac agacggatgg tattgcttat tatttattaa aatcgaacaa tacacttatt
    41161 taaaaacttc tttcccagat tttcttccat ttttacagtt ataactagta ttatcatgaa
    41221 aaatagcctt ttattgtcta tgggaataat aatttaaaat tggtactttt ttattaatta
    41281 gttcatcgcc caataatctc tccagtttct tagcatctcc tgactcaacc ggaattatcc
    41341 aagcccagtc taaataattt tttaaaatct catcatattt tgcaaatatt tcattgccat
    41401 attccgtcct taaaattata ttttcagtgc cagacaagtg atttatattg agttgataaa
    41461 aaagatctcc atctaaaaaa atatcattag aagaagattt ccccacagta atgaccattc
    41521 catcagctct atgtgttaca tacaaataat tgtatgacct catttcacct aatgaatcaa
    41581 ttatttcttg ccctttagct ctaatatatt gatctctatt acctatctca ctcttattta
    41641 acaaaagttt tttcatgtca aatttaatcg gctgttttat ttttgtaaat tcgcttctac
    41701 tactaatcca atccgagatt aatttttcaa catgtcccag agtaaactct gatttattcg
    41761 ctaaatactt tttttgttgt attaaagaca tctttccccg aagctcaaaa ttgagcctac
    41821 atcgattctc tatctcttca aaaagcatct tatcttcatc attcttatat tcatcattca
    41881 taaaaccaat ccagctcctc gattaagata atattttaat ctcatacaaa aactagctct
    41941 catcaaaaca accattgttt tgatttctca tgaaatttct ttatactttt ctccgattat
    42001 aaattcctaa ccccttgaga aaattacgca tatatttatc tccgcaaggc ttaaaatttc
    42061 gttggccttc ttttctcaaa aatgccccga tttcgccttt tgatggatag atttctgcct
    42121 cagcaaaaac gtctaaaata tcatcagtgg tcatagacaa ggcgattttt aattttttta
    42181 gcaacacatt gttgatatat ttgatttcct tgcttttttg cggcgtgtct actatcggct
    42241 tatctttttg cggaccacgc gccaagagaa tcaggccatt taggaatgcg tcaaaaactt
    42301 ggttgttgat ggttttttca tacacattat tttctgcatt atctttgttc ttatcctgta
    42361 ctttggtcag taccgcctga gcttcttctt tagtcagtgg tacaccgcct agctcaaaag
    42421 ctttaatcaa atccgtatct ttcaaatcaa tcgcataacg caatcgcaat aacctatcat
    42481 tgttattcat ttgttcctcc gaagtattta gtctttactt tcttacgtac tttttgtctt
    42541 gctaagattt ttaggatcaa tgataaaaac cgcatcactt atgataaggc gctgggtatc
    42601 atgagtgaat gatattgctg aatacaaata gtccgaccac attaagttga tcggacttca
    42661 taagcatttt attgactaat acttttttgc ttaagaattg attctaacat cttttgctaa
    42721 agttaagcat cctatagttc ctgcattatc tgctaactta ggtgtgacaa tgtattcttc
    42781 gatatttggc gtatcgacat agccgttaat tagttctaaa aaacgtcttt gtactttctt
    42841 tttcagatgt tcttgttgca tgacaccacc accaaaaatt ataatatctg gtgaaagcat
    42901 cagggtcgta ttacaggcac attgtgcaat ataatccgcc tcgatttccc agaacggatc
    42961 ttctatagaa agatcttgtc ccttggttcc cgtacgcatc tcgatagcgg gtccagatgc
    43021 caacccttcg agacaatcat gatgaaaagg acaagtacca gagaaagtat ctcctgaatg
    43081 tctcttaact aacgtatgcc ccatttctgg atggctaaac ccttcaacaa aaatcccatc
    43141 ttgtatagct cctccaccaa ttccagttcc aacagtgtaa taaacaacac tggataaacc
    43201 ttttccatga cctgaaacat actccccgta acaagcagca ttcacatcgg ttgtccaggc
    43261 aattggaatt tcgaaatgct tattcaacgt tccaacaaaa tcaaaatttt tccaggcagt
    43321 ttttggagta gaggtgatat gtccataggt ctttgattca cgatggatat caatcggtcc
    43381 aaatgaccct actccaatgc tttctaattg ttctttatag tgattaaaga aatcaattac
    43441 caaactcatt gtttcattcg gtgtagttgt aggaaaactc actctttcta aaacctttaa
    43501 atcatcattg ccaatggcac aaacaaattt agtcccacct gcttctattg agccatacat
    43561 ttcttactcc accgtccttt tatctaggaa ttcgttcttc cctgtctaat agtttttcga
    43621 aaaactcatg tggttccttt tggtaccaat aggcaacaga agaaatatcg tcttgacgct
    43681 cataaagctt gatgtcatca tttcctaggg tttggaaagt aacacgtaaa ttttctttga
    43741 aactaatggg gtcgggtaaa tgccagcggt acaacccatg catcggtaaa gcatcatcgc
    43801 caaaaccatg aactggattt ggattaggtt ttccagcact aaaataatcc cgggtgtgat
    43861 ctcttttaga ttgaaaagga tagcccataa atagcgtatt aaaattttga gcatatgccc
    43921 tttctccaaa gttcttttta tggaaagccc aagcgccgcc aaaataatct tctgctcctg
    43981 tagatgttat tgtagggaaa tcttcatctc catctatata aaacttaaat tctccttccc
    44041 cccaccagta tctttccaat gcgcacaaag caaagtaggt tcctacataa tacccagtgc
    44101 ctttgattcc atcaataagg gtgtaatctt ttccttttgt cgtgatgggt tcacggttcc
    44161 atttagcatg aaaatacatc gcatcatccg gtacttcagt caatgcataa ttaaatgtat
    44221 aaaaaatgtg cttaataaac tcaggatgtt cattcgttaa agtaatctta gctttttttc
    44281 gaaatggcat ctcaaaatag caattcatcc ctgatgttgg attcactacg attggtaagg
    44341 aattcacttc gcatcttgtc ccaaacccat tacaaaaaaa atctcctaat ggactttcta
    44401 cagaaggagt ggattcatca tcccaataaa tacgcaaaat taaatcacgt aagacgaaac
    44461 tcccagcgat tgttttgtct gtcactgtca tccaaaaatg acgtaaaatt ccagggcctt
    44521 ccatttctgc taaagtaatt gtctctccag catttatcgg aacagaaggt gacccttttc
    44581 tcccaattcc tagatgactc gctgtcattg ccgctttccc tttttcacca tttttatttt
    44641 ctggtgaaat agttctactt tcctctccct tgtggagcca tatattattt aaacaattca
    44701 ttttattcgt cctctatcct ttcttcactt ttttgattat tcatatagat gatttgttgt
    44761 tgtgcctccg caataattgc tccaatatct aaattatcat ttcctaactg caaagcgatt
    44821 gacattgcca agggaagatt cattcctgca attacgtgca ctctgtcaga aatataggta
    44881 aaaaactttt gatttacact tcctccagcc atgtcagtta aaacaattat ttcgtctgta
    44941 gtatccacta actctttaat ttgttcatct atatcatcaa tttcttcgac atatgcacat
    45001 aagctactaa tttgattatg catacctgtt aaaaactcta gtgtctcttt aaaaccctca
    45061 gctagtattg aatggctagc aatgatcaat cttctcataa ttcaaatcct tttcctttac
    45121 gctaatattc caaatgcagc cataaccatc gcaaaaacaa taacgagtag aatcaaaatg
    45181 gtcatactga tttttttctt actgagtaga taatatgctg cagcagtaat aattactgga
    45241 attaatgttg gtaaaatttt gtccaacatt ggttggattt tcatactaac ttcaccaaaa
    45301 gtgaatttaa gtggcgttga aacactgata actgaagcaa ttaatgatcc aactactgtt
    45361 agccccaata ctgaagcagc ttcagttaaa taattaatct tttcaccgta ttcagtaaca
    45421 atcttcattc cctgcttata accaaattca aagcctctca ttcggaaaaa gaagattacc
    45481 atgattaaaa tgaaccataa aaagattcct acaggattat tttctaaagc catataagcc
    45541 gcaattgatc ccatgatggt tgggatcatc gtcatcaaaa gagagtcccc aataccagca
    45601 attggaccca tcgtaccaac tttaaagtca ttaatcgcat cttttgcttc taatccttgc
    45661 ttttcctcca ttgcaacaca agcacctaaa ataatggctg ccagccaagg ctgtgtattg
    45721 taatactgaa agtgattatt taaagcttcg atgtattcat catcatcttt gtataatttt
    45781 cttaaaatag gtgacaatgc gtaagtcaca gatgcacctt gctgtgtttg ataattaaag
    45841 gtacaaactg acatcaacca acgatagttc gctttcctaa gatccttttt cgttacttga
    45901 tatttttcgc tcattttatt catcctcccc aataaaccca acatcagaag ctcccgcaga
    45961 agcagtgatt tgtgcatctt tttcagctct atgatacaca ttaaatgcta aagcgaatcc
    46021 tatgatcgcc gctccaataa taggtacctt caaaaaagcg gctaagaaaa atccaacaag
    46081 taaataacta atgtatcgac ctgtaggcat aaagcgtaac aacatcgcaa taccaacagc
    46141 aggcaacatt tttccggcta atgttagccc atccataaac caattaggta aaacatccaa
    46201 aacgtaatta acagcagact gtcctaaagt aacggcaata aatactggaa ttgctgcaga
    46261 taaaccaaag aaaattggcc ccaaccacaa aacactattc attttcttaa attctcgttt
    46321 attcgcatat tcctgtgatt tgcgaacaac aaaaccattt aagattttga caattacatc
    46381 tagttgcacc cctaacatac ctacaggtag tcctattgct aatccggctt ctactccaag
    46441 ctggctacca aatgccgttg caataattgc gcacgttcca tagtccggta cagatgagcc
    46501 accaaaaccg gcaactccta atgacatcaa ttgaactgta ccgccaacta tcaaacctgt
    46561 ttttatatca cccatcaaaa ttcctgtgaa taatccccaa aaaactgcgt aatttacaaa
    46621 gatcatcgga atcccataat aatcaacatg tttaataaat gctaatatag tcagcataat
    46681 aatttgaaaa atagtaaaat gcattatcct tactccttta tcctaatttt gattgaattt
    46741 cctgtgcctt atccgccgga acaaattgtg taacaatttc tacaccagaa tccaacaatt
    46801 ttttatacgt gctgatattt tcatctgatg cataggctct agaagatagc tgtaatgcat
    46861 tatctttaac aacagtaccg ccaacaatta gtcggtcaat tctaataccg ttatctacta
    46921 aatgaaggat tgtttccggc tctttcacga tgacaaatac tttttcctga tcgtatttcc
    46981 cctttttaaa attcgcaatc gctgtttctt cagtgataat tgatacagcc attccagcag
    47041 gtctagctaa acgcatactt gatttttgga tgtcgttatt tgcgacagaa tcatcgatca
    47101 ccatcactct gtttgcttta gacattggtg cccattgcgt gacaataatc ccatgcaata
    47161 aacggttatc aactctcgac ataacaatac tcataaatac ttcctccttt atctttcatt
    47221 ttcttacaag caattaccat gccaaaacaa aaagaacttg ataaatcaag ctctttttac
    47281 ataatcataa acgtatttaa cctcatgaat cgggatgtcg acactataat aatgttcaac
    47341 gacactgaaa gattgcttaa aatgtgtctg aaatttttga aattcctcac tcgtttcatt
    47401 gaaataaatt tcatcttcca aagtattttg ttttactaat cgttcaatta agcaactaat
    47461 gtgaacatat aatccgaaac acgtattatt atcaaatgaa attcctagat cattttgtaa
    47521 gatgtatata gcatctgaaa cccgttctaa taatttagtc ggatttaata tggtcaattg
    47581 ctccattaaa ttcgttagac taaaattctt cagaatatta ttgttcaaaa ctttcagttc
    47641 attatcatta aagtgtctat tcaattgatt ggagaattta cttatattca ttcctaaaat
    47701 catatcttca attgaaaaga attctacatc ttctagatgt ggatttagtg ttccaacaat
    47761 aaaaagaata ttgttctctt tacgaagctc cgataaatag ttttcattca ctaaagagta
    47821 atagtcacac gtttctatga caagatcagt atcccttgga aagctatctt caagtatctt
    47881 ttttaattta aacgaagttc ctaatccgct tgcacaagaa cagacaatca tatccttctt
    47941 aatatttgat gattcataaa tataatccac attttgattt tcttttactt gttcaagaat
    48001 ctgtttaagc ggtaaatcta ataatatctg atttgcaaca tcaatagcca ttcttgttga
    48061 aacattgttt agtagtccga attttttttg agcgtctagt tccaaattta gataaatttt
    48121 ctccagtgaa cccatatcaa caagtaaaat aatttcttca tatccggtac actcttgtaa
    48181 gtatctatta attttgtctg caatctcact acttgaagtc attagtggca tatcaatggc
    48241 gtcgtatata tatctctcaa cgatcctatt agcagtactt gctatacagg ttgccgttag
    48301 atctccatga gccaaaatca gtgctaaacg tttttgctga aattctttac tacacattaa
    48361 cttaaataaa ataaccaaaa catttttttg aatttttgtt aaataaatat cccctatttg
    48421 aataaaacga ttgactaatt gaacttctcg actacaccaa ctattaatat cattgatgaa
    48481 ggaatataat ttatcgtccg atgttaattg gatttgataa atccaagaat tcaaaaagat
    48541 agacgttatt tgaagataat aatagtcaaa ttctatttga aaatctctct ctatttcctt
    48601 aacatactgt ctaaatcgaa tctcttgata ggtgggatat gttaaagctg aattattttg
    48661 gatatcaaca ttcttttgtc taaaatatct atcatatttc tcaaaaagct gctgcttatc
    48721 agtgtctttc ttccacgttt gataaagata ctctataagt tcgttgatat taaaaacttc
    48781 gtctactttt tccaaatcat ccatttgaat atattttaat tcattctgtc ttttcataac
    48841 cttcatgccg ttgaaattca cccattgtgg taaatgatac atctgaatat aaagttcttc
    48901 caaatcatct aagaaataaa aggcattggc acaggtcaat tgaatgatat ttttcaaatt
    48961 gccaacattt tcactaaagc gatagtttaa taaaaaatct aataattgtt gagatatcag
    49021 tattttcttt gctagtcttt cttcttctga tttaaaaaat atcttgatta actgttttct
    49081 ttcataaaga gaacgactgt ctaatgttgg aagtgttagc tttacaggta ttcttctcaa
    49141 caaagttgat aatagtttat cctcaggtgg ttctgatgtt gcaaatataa tccagcaact
    49201 tgatgtgtac cagttttcat tatcacccat ccgatgaaat ttcctcgtat ccataaactg
    49261 aaaaagtttc tcctgacatt cattgctcaa tgcatgaatt tcatctaaaa ataagactcc
    49321 attattagct aattctgcta aaccctttct tccatcttcg gctcctgtat atgcaccttt
    49381 tacatgccca aaaaaattgg acaagaaaag ttctggatta ttggcatatt cactacaatt
    49441 aactgaaatg aaagatgcct tctcttctat ataattattg gccaacaaat ccttatacac
    49501 ataatttgct aaatagcttt ttccagttcc agtctcgcca tgaagtaaaa tgggtaagcc
    49561 atttggcgga tagtagattg ctgctttaat ttgagcaatt acatttcgca gacttccttg
    49621 acttccgata accctttcta ttccaaccct aaaagagcta tcataccatt tttccgcttc
    49681 ctctgaagag ctaaaaattt gttgatttgg aactttctcc ggtaaagaaa agaagattac
    49741 tggacgagta ttgatttttc caaattctcc atcttttacg aattcattca aatattgaga
    49801 gacaatatta cgactaacat ttaatttttc agatattttc tgtgctgtat tgttccccca
    49861 tttattttta atctcagagt tctctatgac ataattgaca atgcgttctt ttaattcttc
    49921 catcacagca cctcctcgac actattcgaa ttttgccgac agtatttttc cataaatatg
    49981 acttgctaaa aaggctttgt ttagatcatt caattggaac gttgcttgtt ttgcttgatc
    50041 cgctaagacg ttgaaaagat actctggcga aatcgcatac cccaaaatat caacgattgt
    50101 tgcaatcaga tcttcttttg attcttcatc tgaaagtaat tcacgataca acatcgtttg
    50161 tttgcttact gtgtcatatt cctctaagga ttcatctgct aaaagaacca cttgttccaa
    50221 caaacggtcg gataaatatt tatagaaaat cagccccaat aaataatttt tatattctga
    50281 cgcatccatt ttactacgga gattatctgc cgcactaaat aacttctgat ttaattctgc
    50341 acccaaaaag atacactcct atctcttatt taaagttgct atataccctc ttattttagc
    50401 atactcagtt ttagaaaaag caaaaaaata aagaaactta ttcttgataa caagctacgt
    50461 gacgtatatc cctaacacaa aaatagccat atcctttggt ttggatacgg ctatttgata
    50521 ttactcattc aattatcaaa tcacagtagg tgtgaatctc atcgggatcg gcaatatcta
    50581 cttctgatta ggatcaaagg tgattttctc aataaaggtg tgatacaaag ctttaacttg
    50641 cttcttctca ctctggataa gaaagcaatc caaactagtc aatatctgtt ggacatcttt
    50701 gacttgaatc ggttcccctt gttgttgaaa gatccctaag atttcattct ctcgaatttg
    50761 taactgtctt cgtttttcag tcaattcatt taaacgatct tttaacatcg gaaacaattc
    50821 gggactatct tccaaagcag cttcctgttt ttgctctaat agttagattt tgcgtaccat
    50881 tttcttcttt tattttcctt cgattgctga ttttaatata gtctaaagga aatttatatt
    50941 tattgtattc tttataactt caagagaact tcgtttgagt attactgtat ttttttaatt
    51001 ccataagaaa attagttatt acctgccacc taatcagcaa atctgaacct gagtcaatat
    51061 aattaatatt attttccatc tccaacattg taagtactgt tggtgttaat atagggactt
    51121 tgtacaattg tatttttttg attttatcat ctgcacaaag gagatttttt aaatctaatc
    51181 cttcttttat ttcaaaagtt gaatttttta aatctataag agtaaaacca attccttcat
    51241 actgaatttc tgaaaatcga atttttctcc cagttgatag atcaaggggc agaaaacctt
    51301 ttattacatt gtgagtgtca ccatcattta caatgtcatt aggaattttg attttagaat
    51361 tctgtggagc acctattgga agataataat tcattataat tttgtagtct tcatgaggaa
    51421 ataaagtact tttaggattg ctaagatatt ctcgtaccga atctaagaat ggcttatcca
    51481 ttgaatcttc atccccattt aaactaaaaa acattcctac aattgctttt gcaactctaa
    51541 gtgaattcat tttctttatt gaatgtgtta aatcagactt accattcttt aaacttgtat
    51601 tgttataaag gaaattttta ttgtcttctg caaatctaat atattcctca acatactcac
    51661 taccaaaaaa actattacat ttactacata acg
//
LOCUS       DAYYFU010000014        51238 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000014 DAYYFU010000000
VERSION     DAYYFU010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.014"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            330..1175
                     /locus_tag="ACHA34_000993"
     CDS             330..1175
                     /locus_tag="ACHA34_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8791652.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="ACHA34_000994"
     CDS             1456..2676
                     /locus_tag="ACHA34_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8791653.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="ACHA34_000995"
     CDS             2673..5393
                     /locus_tag="ACHA34_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8791654.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="ACHA34_000996"
     CDS             5377..6321
                     /locus_tag="ACHA34_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8791655.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="ACHA34_000997"
     CDS             complement(6390..7400)
                     /locus_tag="ACHA34_000997"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8791656.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="ACHA34_000998"
     CDS             complement(7580..7924)
                     /locus_tag="ACHA34_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8791657.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="ACHA34_000999"
     CDS             complement(7917..8351)
                     /locus_tag="ACHA34_000999"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFM8791658.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="ACHA34_001000"
     CDS             8489..9226
                     /locus_tag="ACHA34_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8791659.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGDIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="ACHA34_001001"
     CDS             9219..10430
                     /locus_tag="ACHA34_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8791660.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10634..11416
                     /locus_tag="ACHA34_001002"
     CDS             10634..11416
                     /locus_tag="ACHA34_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFM8791661.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="ACHA34_001003"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="ACHA34_001003"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFM8791662.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="ACHA34_001004"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="ACHA34_001004"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFM8791663.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="comGA"
                     /locus_tag="ACHA34_001005"
     CDS             14132..15061
                     /gene="comGA"
                     /locus_tag="ACHA34_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFM8791664.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /gene="comGB"
                     /locus_tag="ACHA34_001006"
     CDS             15018..16067
                     /gene="comGB"
                     /locus_tag="ACHA34_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFM8791665.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /gene="comGC"
                     /locus_tag="ACHA34_001007"
     CDS             16064..16342
                     /gene="comGC"
                     /locus_tag="ACHA34_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFM8791666.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /gene="comGD"
                     /locus_tag="ACHA34_001008"
     CDS             16317..16787
                     /gene="comGD"
                     /locus_tag="ACHA34_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFM8791667.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16759..17106
                     /locus_tag="ACHA34_001009"
     CDS             16759..17106
                     /locus_tag="ACHA34_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791668.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            17069..17494
                     /gene="comGF"
                     /locus_tag="ACHA34_001010"
     CDS             17069..17494
                     /gene="comGF"
                     /locus_tag="ACHA34_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFM8791669.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /gene="comGG"
                     /locus_tag="ACHA34_001011"
     CDS             17466..17825
                     /gene="comGG"
                     /locus_tag="ACHA34_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFM8791670.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="ACHA34_001012"
     CDS             17997..19004
                     /locus_tag="ACHA34_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8791671.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="ACHA34_001013"
     CDS             19029..20213
                     /locus_tag="ACHA34_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFM8791672.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /gene="abc-f"
                     /locus_tag="ACHA34_001014"
     CDS             complement(20650..22233)
                     /gene="abc-f"
                     /locus_tag="ACHA34_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFM8791673.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22338..22466)
                     /locus_tag="ACHA34_001015"
     CDS             complement(22338..22466)
                     /locus_tag="ACHA34_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791674.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(22811..22954)
                     /locus_tag="ACHA34_001016"
     CDS             complement(22811..22954)
                     /locus_tag="ACHA34_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFM8791675.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23176..23655)
                     /locus_tag="ACHA34_001017"
     CDS             complement(23176..23655)
                     /locus_tag="ACHA34_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8791676.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23728..24012)
                     /locus_tag="ACHA34_001018"
     CDS             complement(23728..24012)
                     /locus_tag="ACHA34_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791677.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24090..24284)
                     /gene="ssrS"
                     /locus_tag="ACHA34_001019"
     ncRNA           complement(24090..24284)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHA34_001019"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24356..25642)
                     /locus_tag="ACHA34_001020"
     CDS             complement(24356..25642)
                     /locus_tag="ACHA34_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFM8791678.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25780..26451
                     /locus_tag="ACHA34_001021"
     CDS             25780..26451
                     /locus_tag="ACHA34_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFM8791679.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26417..26905
                     /locus_tag="ACHA34_001022"
     CDS             26417..26905
                     /locus_tag="ACHA34_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFM8791680.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26918..27865
                     /gene="prmA"
                     /locus_tag="ACHA34_001023"
     CDS             26918..27865
                     /gene="prmA"
                     /locus_tag="ACHA34_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFM8791681.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27866..28630
                     /locus_tag="ACHA34_001024"
     CDS             27866..28630
                     /locus_tag="ACHA34_001024"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFM8791682.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28863..31076
                     /locus_tag="ACHA34_001025"
     CDS             28863..31076
                     /locus_tag="ACHA34_001025"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFM8791683.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31087..31533
                     /gene="dtd"
                     /locus_tag="ACHA34_001026"
     CDS             31087..31533
                     /gene="dtd"
                     /locus_tag="ACHA34_001026"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFM8791684.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31704..32963
                     /locus_tag="ACHA34_001027"
     CDS             31704..32963
                     /locus_tag="ACHA34_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFM8791685.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33052..33999)
                     /locus_tag="ACHA34_001028"
     CDS             complement(33052..33999)
                     /locus_tag="ACHA34_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8791686.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34393..35685
                     /gene="hisS"
                     /locus_tag="ACHA34_001029"
     CDS             34393..35685
                     /gene="hisS"
                     /locus_tag="ACHA34_001029"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFM8791687.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35700..37469
                     /gene="aspS"
                     /locus_tag="ACHA34_001030"
     CDS             35700..37469
                     /gene="aspS"
                     /locus_tag="ACHA34_001030"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFM8791688.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37578..38453
                     /locus_tag="ACHA34_001031"
     CDS             37578..38453
                     /locus_tag="ACHA34_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8791689.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38719..40602
                     /locus_tag="ACHA34_001032"
     CDS             38719..40602
                     /locus_tag="ACHA34_001032"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8791690.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40677..40961)
                     /locus_tag="ACHA34_001033"
     CDS             complement(40677..40961)
                     /locus_tag="ACHA34_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8791691.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40951..41685)
                     /locus_tag="ACHA34_001034"
     CDS             complement(40951..41685)
                     /locus_tag="ACHA34_001034"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFM8791692.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41814..42437
                     /locus_tag="ACHA34_001035"
     CDS             41814..42437
                     /locus_tag="ACHA34_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFM8791693.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42629..43750
                     /locus_tag="ACHA34_001036"
     CDS             42629..43750
                     /locus_tag="ACHA34_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8791694.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43757..46885
                     /locus_tag="ACHA34_001037"
     CDS             43757..46885
                     /locus_tag="ACHA34_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HFM8791695.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46904..50098
                     /locus_tag="ACHA34_001038"
     CDS             46904..50098
                     /locus_tag="ACHA34_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFM8791696.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50244..50951)
                     /locus_tag="ACHA34_001039"
     CDS             complement(50244..50951)
                     /locus_tag="ACHA34_001039"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8791697.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16771 a         8945 c        10639 g        14883 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc ttgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg gggatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatctcaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggctt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cgggacagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg aaactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acagtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatat cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccctggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttat
    23041 tttacttgaa ctcattgact gttttccctt tattttaaac attataaaga ccgatgagtg
    23101 cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa aaaaacctgc cgatagttgg
    23161 caggtcgtct acgtgttaag ctttggtgtg gtctttagct ggcaattggt tttctaaatc
    23221 agttcggaca acaagcacat cgcaagctgc gtttctaata acatattcag aaacagaacc
    23281 aataaataaa cgttctacag cattcaatcc tgtggctcct aacataatca aatctacttg
    23341 attatcttct gggatttctc tggcaataat ttgttttggt gagccatatt ctacgacaga
    23401 ggttacatta ttcaagccag ctttcttcgc attgctttca taatctgcca atgtttgctt
    23461 tgccatttca gtagcttgtt ctgcaagcat tccatcaaag gatgaaacgg tttggaaaga
    23521 acgtgtgtca atgacatgtg ctaatagaag ttctccgtta tttcggatag ctacattgac
    23581 cgccttttta aaagctaatt cagcttcatc agaaccatct acggcaacca tgattttttt
    23641 gtattgttgt aacataagca ttcactcctc aagtcaatcg tattgtactc ttttattgta
    23701 gaacacttta gggaaaaaga gaattattta ctgctttggt tcagcaattt tttttgcaaa
    23761 tcggatactg aaggtggcag aaacaataat tacgatcagt aaaaagacca aggcaataaa
    23821 gcgttgattg aaaaaagctg ttaaaatacc cattacgcct aaaacagcat atgcagcacc
    23881 gtaaaattgt aggaatcctt gatttttatc atttttgttg atcaatacaa aaaagatatc
    23941 ctgtttatta aataaataat agctaacgaa taataatact aaagtggcga taatcattaa
    24001 aatcctaatc atgaacaaat cctccaaaat aagcaacaaa tagaaaaagc aatttgccaa
    24061 gagcaaattg ctttattctt taaaagatgg taatctccgt taatgccgtt gtttgtcctg
    24121 tagtattgat cccgcgaatt caagcaggtg ggtcccatgg tcgtagcttc gaactaccaa
    24181 atgataaggc atgttaccag cagtgacaca agacgggtcc caagatatca ataaaaatgt
    24241 tcggtcaaca cacaaagagg acaattcgaa catcacagat ttcccacctc tatagtagca
    24301 tatgaattta aaaagagcaa tgagagagct cgcagaaaca gaaccgtttt cttgtctagt
    24361 tttttggagg atgctttttc cactctttta tacggtaata ctgttgaccc agagcctgtt
    24421 catatttccc tgtatctttt ggctgataat actgagcatt cttcagttta tctggtaaat
    24481 attgctgatt gacccaagcc tgatcaaaat gatgaggata ctgataaccc acgccacggt
    24541 tgagtgattt cgcacctttg taatggctat ctctcaaatg atcaggaaca tctccagctt
    24601 tgccctctcg aatatcagca agtgctgcat ctaatgccat gtaggcagaa ttggattttg
    24661 gcgacaagca aagatcgacg acgacgtctg ctagtgggat acgtgcctca ggcaatccca
    24721 atttttcagc agctaataca gcgttcactg tacgagccgc agcagcagga ttacctaaac
    24781 ctatatcttc ataaccgata accatcagac gccggcaaat acttgccaga tcacctgcct
    24841 caactaaacg ggcgagataa tgaagtgcag catccacatc acttccacga atcgattttt
    24901 gaaaagccga aataacgtca taatgagcat ctccattttt gtcatgggta agagcttttc
    24961 tttggacaca ttcttcaatg atcgacagtg taagatgaat ccttccttct ttatctgaag
    25021 gagtcgatag tgtagccagt tccagcccat tcaatgcact ccgcaaatcg ccgttcgttg
    25081 cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat tgcttgttgt ccaaggccgc
    25141 gttctttatc ccttaaagca tgttcgactg ccaactggat gtcttgttct gttaatggtt
    25201 tgacttcaaa gatctgtgta cggctgcgga tcgctggatt gatggtaata taaggattct
    25261 cagtagtcgc tccgatcaag atgattcggc cactttctaa atgaggaagc agaaaatcct
    25321 gttttgtttt atctaatcgg tgtacttcat ccaataagag gatcactgta ccactcattt
    25381 ttgcttcttc cgctacaact tgtagatcct tttttgaatc tgtagcggcg tttagcatgc
    25441 gaaatgcata attagtagag ccggcgatcg cacttgcgat actcgttttt ccagtaccag
    25501 gaggtccata tagaatcatg gaagagagca tgcgagcatc gaccatgcgg cgtatgattt
    25561 ttccttcccc gactagatgc tgctgaccaa cgacttcatc gatcgttcga ggtctcatgc
    25621 gataagccaa tggctgttgc ataggatttt cctccttctt gtttttacgt cccttaatta
    25681 tatcatattt agcaagatgc ctagaaagaa cagccttctg ttgttttgtt ttcatagctt
    25741 atccaactaa tattgtgtat gataaagtag gtgagaaaaa tgacggataa acaaaagata
    25801 gatgaatttt tttggaataa aacgacgcca gaagtggcac gggatttatt ggggatgtat
    25861 ttggagcatg acacaccaga aggaagatta gctggatata ttgtagacgc ggaagcatac
    25921 ctaggtccag aagacgaggc cgcccatagc tatggtttaa gaagaacacc gcgtgtgcgg
    25981 gcaatgtatg aaaaaccagg tacgatctac ctttacacga tgcatactca tcggattttg
    26041 aacatcatta ctcagccaga agggattcct caaggagtaa tgatacgtgc aatcgaaccc
    26101 gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa aaactggtcc agatatcagc
    26161 aacggtccgg gaaaattagt ggaagcatta gcaattcctc aagaacttta tggtcagtct
    26221 atagcagata gttcgttacg cttagttttt gagaaaaaaa agacaccaaa aaagatcaat
    26281 gctttgccta gaatcggtat cccaaacaaa ggtgtatgga cagaaaagcc gttgcgtttt
    26341 gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa accaaatcga gaaagattgg
    26401 ggttggagga aagaaaatga aaaagaagga tctattaaca tatttagatg aaataattga
    26461 aaaacaagtg acagactatg atgtagcttt agattgggat acaaaaaatc atacgatcga
    26521 gatcgttgtt cgcttgtttg cagaaaataa agcacaattt gagattgatg atgcagaagg
    26581 gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc gtacttcttt ttaatcctca
    26641 gaaatctgtt tttgatgaac gcaattatct agcagtaatc ccttatgaag gaaaaaaagg
    26701 gttggcaaaa tcagtagctg attctttagt ggaatatcta aatgaagtat tagctcaagg
    26761 gcaaagcgat ttgcttgatt ttttggatga agacgatgac gaagctgtat tcgagcttca
    26821 ctgggataat caacaactag cagaattagt agaagtaaaa cagcaaaatg aagcagatac
    26881 ttatttaccg tacccaagtt attaaggagt gacagatatg aaatggaatg aagtaaaagt
    26941 agaaactgaa agtgaagcag tcgaagcagt tgctaatatt ttgatggagg caggggcgag
    27001 tggtgtcgcg attgaagatt ctttagatgt tgaacatttt cagtcggatc aatatggtga
    27061 gattttagat aaaaatgatt ttacatcgct gaaagaaggc gcactagtca tggcttattt
    27121 tccggaaacg actttcttac cggaaatctt gccttttatc aaagatcgta tcacacaatt
    27181 acctgagttt ggtctagcta ttggaaaaaa taaagtaaca gttagtgaag tagaggaaag
    27241 caactgggca acagcctgga agaaatacta tcatccggtg cgtattacgc gaatgctgac
    27301 ggttgcgcca agctgggaaa actaccagac agaagatcct ctagaaaaaa ttatcacact
    27361 agatcctggt atggcatttg gcacaggcac tcatcccacg acttccttga cattgacagc
    27421 actagaaaca gtcttgcgtg gtggtgaaac attgctagat gtgggaactg ggtcgggcgt
    27481 gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc tatgcctttg atttagacga
    27541 agtagccgtt cgttcggcga aagaaaatat ggatatgaat gaaatagcca aagatgtcca
    27601 tgtatcagct aacgatcttt tgacaggaat cgagattgaa agtgatgtaa tcgttgcgaa
    27661 cattttagca gatatcatct tattgatgat cccagatgct tggcgtttac tgaagcaaac
    27721 aggcacgctg atcgtttcag gaatcattga agagaagaag caaatggttc tggatgccat
    27781 gttagaacaa gggtttatcg tggatcaaat tttccagcaa aaagactggt atgcaattat
    27841 tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt ttaaaagaat catatgaatc
    27901 aaaagactat tttgttttag aaggggaaaa ctatcaccac attgtacggg ttatgaggat
    27961 ggaacccgag gatgaagttt ttttggcatt caatgatcaa atagccattg ttgcagaaat
    28021 cacggagatt actgaagagg ctgtttatct gaaagaaatt aaaaaagaag aaaatcaaaa
    28081 ggaactgcca agttcgatta ccatagcgag tggctatcct aaaggggaaa agctggattg
    28141 gatcgttcag aaatccactg aattaggagc gcaagctttt cttggtttcc cggcatcagc
    28201 ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga caacaacggc ttgaaaagat
    28261 tgctcaagag gcagcggaac aatcccatcg gcagatcgtg ccatcagtag agttattgga
    28321 aacgaaacaa gatttatttg agcggctaga tgattatgac gtggtcttgg ttgcttatga
    28381 ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa attcttagtg aaacaaaacc
    28441 agatagtcga atcttagctg tctttggacc agaaggtgga ttatcccgcg atgaagtcga
    28501 ttattttgta gaacacaaag ggatactttg tggattagga ccaagaatcc ttcgtacaga
    28561 aacagcacct ctttatttat taagtgctgc tagctatcaa tgggaattaa tgaatccttc
    28621 tttcagctaa cgttcttcat taataaaaga gtatcgacag aaagaattga aaaaatagtc
    28681 aacaatttgt ataatagaaa caattattta tacaatggat acgaaaaatc gtttgaaaaa
    28741 gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac tgcacggatt ttgggacaat
    28801 tatcaaacag atatttgtcc cgattcccgt tttaccgaaa aaagaaaggg ggatcgattc
    28861 ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat aaagctcgtt agtcaataca
    28921 tgggtcccga acatgtagca tttgttgaga aagcatgcga atacgcgact gctgcacatg
    28981 acggacagtt tagaaaatca ggcgaaccct acattattca tcctatccaa gtcgcaggta
    29041 tattagcaga tttaaaaatg gatccccata cagtggctac aggcttctta catgatgttg
    29101 ttgaagatac agaaatcact ttagaagatc tgagagaaga atttggcgat gacgttgcta
    29161 tgttagtgga cggcgtaacc aaattaggga aaataaaata taaatcccac gaagagcagc
    29221 tagcagaaaa ccaccgaaag atgctgctag caatggctca agatttacga gttatcatgg
    29281 tcaaattagc tgatagacta cataacatgc gtacgttgaa gcacctgcga gaagataagc
    29341 aaagaaggat cgctcaggaa actttagaaa tctatgcgcc tcttgcacat cgtctaggga
    29401 tcagccggat caaatgggaa ttagaagata cagcacttcg ttatctaaat ccaaaacagt
    29461 attaccgtat cgtccacttg atgcagacga agagagaaga acgtgaaaaa tacgtaagtg
    29521 gtactgttga agatattcga atagcgacag aagagctggg gatttttgca gaaatctatg
    29581 gacggccaaa acacatttat tcaatctatc gtaaaatgaa agatcagaaa aagcagttca
    29641 acgaaattta tgacttgcta gctattcgag tgatcgtaga ttcaatcaag gattgttatg
    29701 ctgtattagg agcaatccac acaaaatgga aaccaatgcc cggcagattt aaggattata
    29761 ttgccatgcc aaaagctaat atgtaccaat ctttgcatac taccgtgatc ggtccggcag
    29821 gaaatccggt agaaattcaa atccgaacac aggaaatgca tgaaatcgct gaattcgggg
    29881 ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa agtagaacca gatggtatga
    29941 cgaaacaatt aagctggttc catgagatac tcgaacttca agacgaaagc tatgatgctt
    30001 ctgaatttat ggaaggcgta aaaggagata tctttagtga taaagtctac gtcttcacac
    30061 caaaaggaga cgttactgag ttaccaaaag gatccggacc attggacttt gcatacagta
    30121 tccatacaga tatcggtaac aaaaccactg gtgcaaaagt aaatggcaaa atggtgcagc
    30181 ttgattacaa attgaaaaac ggagatatca ttgagatcat gacttctcca aattcatttg
    30241 gcccaagtcg cgactggttg aaattagttg ctactagcaa agcaagaaat aagatcaaac
    30301 gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa aggccacgaa tccgtggtca
    30361 aatgtattac agatctagga tttacgccta aagatatttt gacgaagaac aaactgcaag
    30421 aagcactcga tcgttttaat tatcaaacag aagatgatct ctatgcagct gtagggtatg
    30481 gagaagttag ccccttgacg atggccaatc gtctgactga aaaagaacgt aaagaacaaa
    30541 aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa tcagccgaaa aaagaacctg
    30601 acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca aggggtagaa aacttactaa
    30661 ttcgtatcag tcgctgctgt aatccgattc ctggtgatga tatcgttggt tatatcacta
    30721 aaggcagagg gatatccatt catcgtcgag attgtccgaa tgttcagcct gacaaaccaa
    30781 atgtagcaga acgtttgatt gaagtcgaat gggaagatac atcgaataca cgaaaagagt
    30841 atgatgcaga tttggaaatt tacggctata atcgttcagg cttattgaat gatgtacttc
    30901 aaacagtcaa tgcgctaacg aaaaatctca acagcgttga agcacggacg aataaagata
    30961 aaatggcgac gatccatttg acggttggta tccagaattt atcccaccta aagagtatcg
    31021 tggataaaat caaagcagta cctgatgtct acagtgtacg ccggacgaat ggataggagg
    31081 acagatatga gagcagttgt tcaacgggta agtaaagctg ctgtgtcagt cgatcagcaa
    31141 gaaattggaa aaattggcta cggcctactt gttttattag gcattcatga aaccgatacg
    31201 caaaaagatg tggactatct ggtaaaaaaa attgcacaaa tgcgtatttt tgaagatgaa
    31261 caaggaaaaa tgaatattag tgtagaagat gtgggaggac aaattttaag tatctctcaa
    31321 ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa gctttgtgga agccgcacga
    31381 ccagaaacag ctattccttt atacgaatca ttcaatgaag gaatcagaag tcgcggcatc
    31441 acagtcgcta ccggacaatt cggagcagat atgtcggttt ctctcgtcaa tgatggacct
    31501 gtaacgatca ttattgattc taaaaacaaa taaagtaatc tggttttcac tagtaaaaac
    31561 tatgaaacac gtcgcttaag ttagactgat aaactctaac ttaagtggct tttttgtctg
    31621 caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta agcttaaatt atacaagtta
    31681 ttttttatag gaggttgctg aagatgaaag aagtaccagt agtagatatc aaaatcacag
    31741 atttaaaaaa attgtatcaa aaaggataca acatgttgga ggagttacgc catgaagcgg
    31801 acgctcccgt cgtcaaagca aagattttca ataaagaagc tatcacgatc tacggatcat
    31861 cagcagcaaa agtattttat gatccaagga atttcaaacg aaaaggtgct atgccaaaat
    31921 tagtattgaa gacacttttt ggtcaaggtg gagtacagac tttggatgga gctgctcatc
    31981 accatcggaa aaatattttt atggatctaa tgacaccgga aagaatggaa gattatcatc
    32041 gtatcttaga taagaatctg acacaagcac ttgaagcaca gcatggtcaa tttgaactat
    32101 ttgatttaag taagatggtt ttctttacat ctatttgcga gtgggcaggt atcaatttat
    32161 cagcgatttc taaagatgag gtcgaaaaat tagcagaata tcaaatctct atgatcagcg
    32221 gaacgtttac ttctccgatt gatcacataa aaggtgtcga gaatcgcaaa aaatcagaaa
    32281 aatgggcgca aggcttgatt gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg
    32341 tggcattata tgcttttgcc aatgccacag atttagatgg tcagctttta cctctggaag
    32401 tggcagctgt tgaattattg aacatcatcc gtccaacggt tgctctaact gtgtgggcgg
    32461 cattgatggg acatgcactt ttcagtagac cagatttgta tcagcaatta aaaaatgact
    32521 tttcgacgtt acaagatccg tttattcaag aaatgagacg ctattatcct ttcttcccaa
    32581 tgctacctgc gatttcgctg aaagaagtag aagtagatgg ttatcgtata ccagaaggca
    32641 gttgggttat tttagattta tatggaacag accatgatga acgaacagtt gaagcgccag
    32701 attcctttat gatcaaacgg tatgtaggga aagcaaaaga tatttcctat aaagaagaat
    32761 atgagatgat tgcacaaggc gggggaaatt ttcgacaaat gcatcgctgt gcaggagaat
    32821 ggatcactct tcatagtcta cgtgtgtttt ccgatcagct agtaaataag tttgagttct
    32881 ctgtacctga acaagattgg acgattccat tcaatcagtt tccgacttat ccgaatagcc
    32941 gagcgctact ttacaagaat tagattagca taaacttatc gaaaaaaagg aagatcaaac
    33001 agtgctgatc ttcctttttt catattacta taaattgata aggtcaattg atcaaaatcc
    33061 acgaaatgct tcatcaatgg tttgataatc ttcattggat aaatgcaaat ctaaagcttg
    33121 gacattgtca gatacttgct ctggacgctt agctccaggt ataactacag acactcgtgg
    33181 attcttcatg taccaagcta aaacaagttg cgcaggagtg gcttggtatc ttttagcaat
    33241 ggggcgaagt acatcgacag cggttaaaat ttctctgaaa cgttctcctt ggaaatctgg
    33301 atttcttttt cgaggatctc cttcaccaaa ttgtttttcc tcaccaagtt catattttcc
    33361 tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa ctgattttgt ttttttcaag
    33421 ataagggaat aattcttttt cagcctgccg atggatcaaa ctgtatttat cttctactac
    33481 atcgacatat ccatctgcat tcgcttcttt caattgttct aacgtgaagt ttgacacacc
    33541 gactgctcgg atttttcctg cttcttttaa ttcgtgcaat gtagcaactg attcattttt
    33601 tggtgtgctt tcatccggaa agtggatata aaaaatgtcg atgtaatcag tttgcagacg
    33661 ttttagtgca tcttccaccg attgtttgag aaactcagga gaattatcaa atgttcgccc
    33721 tttatttggt acatgagcag cttttgtagc gatgacgaca cgtgagcgat catattcttt
    33781 ttccttcagt acctcgccaa tcagttcttc ggaacggccg tttccgtaag cataagcggt
    33841 atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact acttgttttc ctgtctcatc
    33901 ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt gctcctaatc caagctgttc
    33961 tgcgtagact tgtgaatgtc cgattcttat ttctgccata gctgattcct cctttttgtc
    34021 tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt tttgtgaggg gaaatagtca
    34081 gatatctctt gactttgaga ctggttttta gtaggttata agtaatatcg aatgtcaatg
    34141 aaagagcgag taagtttttg aaactttaca agagaggatc atcgaggctg aaaatgatct
    34201 aaagtttcat tagcctaaag acactctgga gatggacgag tgaaaagccg tcccgcgtac
    34261 gagcgttatt cgtaagagga aagagaaatc tttcaaaatt aggtggtacc gtgtatgaat
    34321 ttcattcacc cttgttcatg atgaacaagg gtgatttttt tatttacata gctaagaaag
    34381 ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa atgatatttt gccaggcgaa
    34441 tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat tattcagaga ttatcagtat
    34501 aatgaaatgc gtacaccgat ttttgaacac tatgaagtga tatcccgaag tgtaggggat
    34561 acaacggata tcgtttcaaa agagatgtat gatttttatg acaaagggga acgccatgtg
    34621 acgcttcgac cagaaggtac agcaccaatc gttcgttctt ttgttgagca taaattgttc
    34681 ggcccagagt atgcgaaacc gttcaaaact ttttatatgg gaccaatgtt tcgctacgaa
    34741 cgccctcaaa aaggacggtt acgtcaattc catcaaatcg gagtagaagc atttggatca
    34801 gaaaaccctg caactgatgt ggagagcatg gtgatggcac ttgatttctt caaacagttg
    34861 gggatcacgc aaatccggct agtcatcaat tcattaggtg ataaagaaac gcgtcaagcc
    34921 tatcgtcaag cattgatcga ctatctttta ccttttgaat cagagctgag tgaagactct
    34981 aaacgccgcc ttcatgaaaa tccattacgc gtattggaca gtaaagacaa acgagatcaa
    35041 aaatttgtgg cagaagctcc atctatctta gattatctaa gcgagcctgc tcaaaaacat
    35101 tttgataccg ttgtttccat gctggatgca ttggctgttc cttatgaaat cgacagtaat
    35161 atggttcgcg gactagatta ttacacacat acgatttttg aaatcatgag tgacgcacca
    35221 aaaatggggg cacaagcaac gatttgcgca ggcggacgtt atgacaatct agtggaagaa
    35281 ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg gtatcgagcg attactcttg
    35341 actatggagg cagaggaagt agttatccct gcatttaatg agttggatgc ttacgtggtc
    35401 gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg tccaagcgat tcgaaacttt
    35461 ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa aagcgcaatt taagacagca
    35521 gataaagcga atgccaaatt agtattggtg atcggtgaag atgagcttgc tagtcaaacg
    35581 attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt ttccattaga agagatatat
    35641 gaacattttg atgaagtcta tgatgaaatg actttattcg agtaggagga aaaaaataaa
    35701 tggcacaaag aacgatctat tgcggattag tttccgaaca acaattagaa caaacagtta
    35761 cattaaaagg atgggtacaa aaaagacgag accttggggg agtaatcttt atcgacctac
    35821 gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga aatcaacaaa gaagcatggg
    35881 aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt gactggggaa gttagaagac
    35941 gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga attcgaagtg attgcaagtg
    36001 aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca aattgaagat gatcaagcaa
    36061 tcagtgacga aatacgtttg aaatatcgtt atttggatct gcgtcgtcca aaaatgacta
    36121 aaaacatcaa attaagaaat gcgacaacaa aagcgattcg tcattattta gatgataatg
    36181 actttctcga tatcgaaaca ccttacctag ggaaatctac accagaaggc gcaagagact
    36241 acctagttcc ttcacgtgtc catgctggac atttctatgc gttgccgcaa tctcctcaat
    36301 tgtttaaaca attgctgatg aatgctggat tcgaccgtta ttatcaaatc gttcgttgtt
    36361 tccgcgatga agatttacgt ggagaccgtc agcctgaatt tacgcaagta gatattgaaa
    36421 caacgttctt aacagcagaa gaaatccaaa cttacacaga gggattgatt gcgaaggtaa
    36481 tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc tcgtatgacc tatgatgaag
    36541 caatggctcg ctacggcagc gacaaaccgg atacacgttt tgatatggaa ttgatcgatc
    36601 taagcgatac agtcaaagaa gtagaattca aagtcttcca aatggctctt gaaaacggcg
    36661 gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg ctactcacga aaagacatgg
    36721 atcagttagg tcaatatgta ggacaattcg gagctaaggg gcttgcatgg ctgaaagtag
    36781 aagaagacgg attgaaaggt ccaattgcta aattcatggg agaagcaaca gaagcaatca
    36841 ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt tggcgcagat aaaagtgaaa
    36901 tcgtcgctgc tgcattaggt gctatccgga cacgtttagg aaaagaatta ggactaattg
    36961 atgaatcgaa attcaatttc ttatgggtaa ctgattggcc acaatttgaa ttttcagaag
    37021 aagaaggtcg atatgtctct gctcaccatc cattcacgat gccaaaagaa gaagatatcc
    37081 cgttattggc tgaagaccct gcaaaagtct atgcagaagc ttatgatatc gtcttgaacg
    37141 gttatgaatt aggcggaggt tctatccgta ttcatactcg tgagcttcaa gaaaaagtac
    37201 tagaaactct aggctttaca aaagaacaaa tggaagaaca atttggtttc ttgttaacag
    37261 ctttagatta cggattccct ccacatggcg gtattgcatt aggattggac cgttttgcaa
    37321 tgttattagc cggagaagat aatattcgtg aagtcattgc ctttcctaaa aacggaaaag
    37381 cagcagatgt gatgagtgat gcaccaagta tggtttcgcc tttacaattg ttcgaattga
    37441 atattgatgt aacgaatata gaagaatagc cgataaaaag gtaaaaagga tttgtcgaat
    37501 acgcttcttt tgacaacctt gatttaattg aaaaacactt tataatgaag ccataacata
    37561 taaagcgggg cttttccatg aaaaaaacat tcgaaaattg ggcttatcat gattacacga
    37621 ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc agtcgcattg aatttctttt
    37681 atcagccagg acatatttat tcaagtggta ttactggtct agctcagatt ttgacgactt
    37741 tgtcaaaatc actgatagga ttcgaagtac cggtatcaac aactttgtat ttactgaata
    37801 tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa gtttacaatt ttcactttta
    37861 tcacagtcac tttgacctcc ctttgtatcc atattttacc ggaaacagtc ctatctaccg
    37921 atccaatcat ctgtgcgttg tttggggggg ctgtgatggg actaggtatt gggctagcgt
    37981 tgagaaacgg cctatcatcg ggcggtctag atatcgtgag tatcacgatt cgtaaaaaaa
    38041 caggaaagtc gattggttct atttcgatct acttcaatgc attgatcgtt tttgttgccg
    38101 gaattctatt tggctggcaa tatatgtttt acagtacgtt gtctattttc gtcagtggaa
    38161 aagtaactga tgtcgtttat actaaacaaa agaagatgca ggtaatgatc gtaacgaaac
    38221 atccagaaaa agtagtaaat gaaatccaaa actgtatgcg tagagggatc acgatcatca
    38281 atgaagcaga aggtgcttat aagcatgata agcagacagt attgatgacg atcgttacac
    38341 gatacgaact gcctctactt agagaagcaa tgaaagcttc tgatccacaa gcttttgtaa
    38401 gtattgcaga aaacgtccag atacttggtc gtttctatga ggatggcatt tagatcgagt
    38461 ttgacaaaaa ttcatgaaat actgctgtag acagacgttc gtttgtctgc agtttttttc
    38521 taccatagta gaatcagcaa agaaaaactg cttttttatt tgaaattttc ttaaagttga
    38581 aaaaaagcat gtttattttt ttaaatttat gggatgatag aaaaaaatag tagagaaagg
    38641 acacgattct acttgctatt ttcacttaat acccgtatat tacgaatgaa aaaagtttat
    38701 gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa aaaacagccg ctacatcact
    38761 gggtttgatg ggctccgttc attagctgtg gtgggtgtta tcttctatca ccttctgcca
    38821 actagtatga aaggcggcta tttgggcgtt ccgatttttt tcgtcgtatc tggttatttg
    38881 atcacggatc tgctgcggca ggaatgggaa caaaatggaa aaatcaatat ttggcaattt
    38941 tatgtcagaa gaatgaagag actctatccg gcacttgttt ttatgcttgt gacagcttcg
    39001 gcttatatca ctttgtttca gcgagggctg ttaaacaatc ttcgaggagt tgtggtcagc
    39061 agcttactgt atgtcaacaa ctggtggcaa atcaataacg gtttatctta tttcgatcga
    39121 tttgctaatg agtcaccgtt tacgcatatc tggtctttag cagtagaagg tcaaaactat
    39181 ttggtttggc cgatcttgtt tgtattgttg atggtatttg ttcgtaaaag aaaatggatc
    39241 ttttatactg tgttaggtac atccttcctc tccgccatct tgatgatgat cttgtataca
    39301 ccaggaggcg atccaacccg tgtttattac ggtaccgaca ctcgtctgtt ttctatatgg
    39361 ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt taaagaaaaa catcccgaaa
    39421 caagcgaaaa atattctgaa tatcgctgga ggaatctcgc ttttggcact gattctttgc
    39481 ttcttctttt tagatgatca ttacaatttc atctattacg gaggaatgct cctagtgagt
    39541 ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg gagcaagttt gaatcgatgg
    39601 ctgacaaatc cagtatttac ttggattggc aaacgaagct atggcattta tctgtatcag
    39661 tttccagtta tgatttttta tgaagcaaag gtgactgata ttgccgatca tgttttgttg
    39721 catactttag tggaaatcac attgattcta atcatcagtg aactttctta tcgatatgtg
    39781 gagcgtccgc tagcacgttt tgattatggt cgtacattcc aagtgatgaa aggatggttc
    39841 agcaaaccga tcatcagtaa gagcaaacca tgggttgttc caggcacatt acttgtagca
    39901 attgcactcg ttggtttcgt tacggcaccg aaaaacgctg taacagccga tcaaaaacaa
    39961 ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag aaaccagaaa caattcagaa
    40021 aacaccacta gttcggttga ccaagcgatc cttgataagt acgagttgtc caaggcacaa
    40081 gggaataagg cacaaaaatt agaactaacg gcatttggtg attcagttat gttggatgct
    40141 gcaacggatc tacaagaact ttatccaaaa gtagtggtag atggagatgt cggacgacaa
    40201 ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac aagatttgtt gaaagacact
    40261 gttctgattg gactaggaac gaatggctca ttctcggaag ctcagtttga tagtgtgatg
    40321 gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc gagtaccgac gaagcgctgg
    40381 caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa aatacgacaa tatgacgctg
    40441 attgattggt acagctacag taatgaccat agtgactggt tttatgatga tcaagtccat
    40501 ccaaatcctg aagggatgac acattacatt cacttagtca gcgaagcctt gctaggagga
    40561 agttcaagta cgaacagcag tgagacaaac gtgacagaat gatagctaca aaaaacttca
    40621 ggcaaaaaca tttgtttttg cctgaagttt ttttatattt accgtgtttt taacattcat
    40681 atagattcag gaagcaaaac ggttgagtgt gtctgtaaat aacgttcttt ttgttttcgg
    40741 gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc ttcttgaaga aaatacttct
    40801 gcatggatca tcttaacagg tcttcttttt tgaagacgag tatatttagc accagtacca
    40861 ttgttatgtt cctctaatcg tctagaaaga tcggttgtat atccaccata aaatgtctga
    40921 tctttacaga ataatacgta aaaataatgt tcatttgcca tagagcatct cacgaatctc
    40981 tgcagtgtat tctccatcca tattgtaagt gatcaatggt ggagcgactt tgaatccgtc
    41041 taatttgcct tctttgattc cttcaactaa caaaatattt gcctcttttc caggttttgg
    41101 gtaaacaaat tggatccttt taggtgcaat tcgatagtct tccatgacct gaatgatttc
    41161 taagaaccga tcaggacgat ggaccatagt aaagcgtcca tttgttttta gtagcttact
    41221 tgaaatatcg atcacttctt ctaacgtcgt atgaatctca tgacgggcga ttgccaggtg
    41281 tttattaggg ttcgttttat ttgttggcag ccctttgaaa taaggtggat tacagacgac
    41341 taagtcacaa gaattatgtc gaacgacaga taagctgtct ttcagatcga tggtgtggac
    41401 agtcatttgt tcatttaaat ggttaagcgc gatactacgt cttgccatgt ctgctagacg
    41461 aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt ttacttaaaa aaagtccaac
    41521 tgcgccattt cctgcacaaa ggtcaacaat cttccctttt ttaggtacac gggaaaagtg
    41581 agcaagcagc acagcatcta aagaaaaaga aaacactttg gaactttgga taatcttgat
    41641 atcttctgcg tataattggt cgattcgttc gtcttcgtga agcatggatt ccctccaaaa
    41701 tgaaaaaaat agtcgtctgt attatactat ttttattaaa aagaaccaag gtggcttgtc
    41761 aaacctctat tttttatgca tactatgacc ataagaaggg aaaggaagag actatgtttt
    41821 atcgatttat gcgcggggta gttcgagtac tattagcctt agttaatgga aatgcccgct
    41881 atgagaataa agaagtttta ccgcaagatg agaattacat actagttgct ccccatcgta
    41941 cttggtggga cccattatat ctggcggtag ctgcagcacc caaggaattc agctttatgg
    42001 caaaagaaga actgttcaaa aatccagttc tgcgatttat attaactcga tccaatgctt
    42061 ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa aacacctgta aagattttga
    42121 aatcgacaaa tttgagtttg atcatgtttc ccagtgggac aagacatgct acagaactta
    42181 aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa aatcgtgcca gctgtatatc
    42241 aaggtccgtt gacacttggg gatctgttca agcgtcagag agtgacagtg cgtttcggcg
    42301 aaccaatcga cttatcagat atcaagaaaa cggataaaga aggtatggcg gagatcgaac
    42361 gacgaacaca agcagctttt gacaaattgg acaaagaagt gaaccctgat ttcaaatatg
    42421 aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg gtcttttgaa tctactgtcc
    42481 tgttcagttc agactttttc aatgaaaacg agggagagac gtcaagtctg ccctcttttt
    42541 gcgttccaat atgtaaaata gaagacaggt gtcatgtaat ggatcaaagt gttataataa
    42601 ttagaaggac attttttgag gtgattgaat gcgtttttta catacagccg attggcatat
    42661 cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag cgtcatgtac tatcttctat
    42721 actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg attgccggag acttatatga
    42781 taggagtgtc ccagctgttg atgccgtaga attatacaat gaattgatgg tcgattggaa
    42841 tttaaaggaa aagatcccga tttttgctat atcaggaaat cacgatagca gtacgagact
    42901 ttcagcgggt acgccatggt tcagttattc gaactatttt ttgcgaacac agctagcaga
    42961 agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc tttcttcctt actttgaacc
    43021 aattgcagcg agattatatt ttgaagatga caatatccga acgatcaagc aagcggtagc
    43081 caaggtgata gataaaatgc aggagctatt tgattcagaa aaaaaacaag tattgattag
    43141 ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa acgaaaatcg aagtaggcgg
    43201 gttggattcg atccctggtg aactacttac accgtttgat tatgtagcac taggacattt
    43261 gcataatcaa gctgctttga gacaggataa tgctcgctat agcggttccc cattgaaatt
    43321 ctctttatct gagatcaacc aaaaaaaagg cgtatggatc gttgacctga caacaggcct
    43381 ggagttgaat tttagggaga tcgaacccct gcatgatatt gaagaaataa cagcgagttt
    43441 caaagaattg ctggatcctg ccttttatca gtccgtcaat cgggagaatt acatctatat
    43501 ctatctcact gatagaacga tcatcccgaa tatgatgaac cagcttcggc aaatatatcc
    43561 tcgtattttg gggattgaac gggcaaacgg tcgagcagaa caaacaaagc gcaaacgaaa
    43621 agtactagaa atgaaagatc ctcaaaaatt agcagaagca ttttttagtg aaatgacgga
    43681 agagcagcta actgcaaacc aagaaaaatg gctcggagaa acgttgcaag aaatcatgga
    43741 aatggaatga tcaagaatga aaccgaaaaa attacaactt aaaaattttg gtccttttat
    43801 caatgagacc gtagattttt cacgtttgac agaagcaccg ctttttttga tcagcgggaa
    43861 gacaggagct ggcaaaacga cgatttttga tgggatcacc ttcgcacttt ttggtgaaac
    43921 ttccggaaga cttcgctctg gaaaagagat gcgttctttg tttgctacac cagacgaaga
    43981 aacaagcgtg actttttctt ttgaacatca gcagatgagc tatgaaatcg aacgaaaacc
    44041 agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa aaacaggcag ccaaagtaac
    44101 gttgaccatt tttgatggga aaggaaaaga aataaaacaa atcacaaaac gtacagaagc
    44161 agatcagttg atcagagaac tgatgaatct cgatgcgaaa cagttttctc aaatcgtttt
    44221 actaccccaa ggagaattcc gtaatttttt ggtttcatca agcagtgata aagagactgt
    44281 tttacgtcat ttattcggta ctcaattttt ccaacagttc aatgagcgat taaaagaaaa
    44341 agcaaaacaa cagcaacaaa aattggatca tttagagcaa gagctgcatc ttctgcaaaa
    44401 aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg tttgaagccg ttttagccca
    44461 atggacaaac catcaggcaa tactcgaaaa acaaatcacg gaagaaaaag aagctctaac
    44521 ggaacttcag gaaaaacaaa agcaactaga aaattcctac tatcgttttg aaagcttgaa
    44581 aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa aaggaattag ctgctcaaaa
    44641 agacgaaata gaggaaaaga aacgatggat tgggtattat gaatttacgc aacgattagt
    44701 cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa atagaactgt cacaaaaaag
    44761 tagtgagcaa acaaagaaac tagcgatgac tacgcagaat tatgaagagt ggcaaaaaaa
    44821 tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa gctgctcaaa aactgcaaaa
    44881 caaacagatg atggtgccaa ttgtcgaatc cttacgtagt aaaaaacaag acgcagaaca
    44941 attaacaatt tcattggata aagaagaatt gaatgccgag aagctgtatc aaaatctcgc
    45001 tatctatcag gaaagaactc gaattcttca agaaaattta ctggatcagg aaaaactgca
    45061 agaagatcgt cttgcttttg gacagttaaa atatctagat gaaaagcagc aagaaataga
    45121 aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata agagaagaac tcaaaaaagc
    45181 aactgaagaa ataacaaaaa atagtcagct attgaaagaa cagacagaac aagcagaaca
    45241 gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt cagctttttt tgcgtccagg
    45301 ggagccatgt cttgtttgtg gatcaacgga acaccccata caacaattaa aacacgaaca
    45361 gccaaataag gaagatatct taaaagtaga acaacaactg gcagaagctg agtctgctgt
    45421 agaaagaact cagcaaatca ttgccaaaag tgaagcacat gaaaataatc ttcaacaaca
    45481 attatcagat ttagaacaaa aaatcatggc aacgaatata caattggctg aacaagaaga
    45541 acacttgcaa caggctttga aaaaggcatt tcctgagata acagaacaaa aaacaaaaac
    45601 aactcttgta gtaatcgaac aatctttaga tagacgaaaa agcaagatgg ccaatgaaag
    45661 aatagagctg gaagaaataa gacaacaagc agaaaagctc gaacaacaat tagctgtcta
    45721 caaacagcag cagacagaaa aaaataatca gctccagcaa ctgcaaggag aaatcaaaag
    45781 tttagaacaa caattgcaag atacggatag taactgttta gaagaggaga tacgacgact
    45841 tgaaaaacag ttagaagaag actcgaatca actagctgtt gagcagaaag caggagtgca
    45901 attgttgcaa gatcttcata cgcttcggac acagcttgat cttcaacatg aacaaataga
    45961 aaaaatagat gaaaaaatag aaaaggaaca agaaagaata gctaaaaagt tagaaagcca
    46021 gtcgtatttt tcaacaattg aagaaatctg tgcatttgca caacagcaag cacaatataa
    46081 agaaaaccaa cgtacagtta cttcttatca tgaagagcaa ttagtgacag ctgaccggct
    46141 gcgtcaatta gaaaatatcc agctaactga gacattccca gatacagaag agatcagcca
    46201 acagttagaa gaaataagac gtatgatcga aataaagcaa gaaaagatat acggtttaca
    46261 agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt ctttcgattt atcacacaag
    46321 ccagacgaaa ttagatgagc ttacgcaatt acagcagctt tcgcaaacat tcaacggaga
    46381 aaatcctaag aagactagtt tggaacggta tgtgcttcag gtttatcttc aagaagtgtt
    46441 acaagtagca aatgatcatc tccaacgttt aacgaaaaat cgttatcaat ttgagctagc
    46501 ggataccatt ggcagttatc gagggaaaac cggattagaa atcaatatct atgatgatca
    46561 ggcaggtatg tctcgaagtg cgcataccct gtctggcggt gagagtttta ttgcagcact
    46621 ttctttagca ttagctcttg ccgatgtgat ccagacacaa gctggtggtg tagcaatcga
    46681 tgcattgttt attgatgaag gatttgggtc attagatgaa gaagctttgg aaatggcaat
    46741 ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc ggcatcatta gtcatgtaag
    46801 agaattgaaa gaacgagtat tacaacaaat aagaattgat acagaaggta gcggacaaag
    46861 caaaatacgc tatcaacttg gataaaaagg gagattggta actatgaaat ggagtattcc
    46921 agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa ggccgagtgt tatcggttgt
    46981 ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg ggcagtgaat tgtatatggt
    47041 tgatttggat ggtacagcaa aagagataga ttactgccaa tgtccctatt gggaggaaca
    47101 taaatattgt aagcatacag ttgctgtgga actttattta cggtcgaaaa atctatctcg
    47161 tatcatgaaa aaagaccagc caatgctaga accggtaaaa gcaacaagtg agggagaaat
    47221 cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat cctcaagcgg tgcaaccttt
    47281 gattattgaa taccaagtag aaacgattga gactaatcag tatcacccag agctttctat
    47341 tttggcactt tacttgcgta tcggttcatt ggcatcaccg aaaaaaacat atatcgtaaa
    47401 aaacatctat gattttttac aagcttacac agaaaaagaa acttacactg ttaataaaca
    47461 gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa gatcaaaaaa tactcacgca
    47521 tcttgcagga agtgctcaga cgcatcaatt attaggtacg aacggcttac aagtcaaagg
    47581 aaaactagat aaaagatatc tgcttctccc agtagaacaa gcccgcttcc tgttagagca
    47641 gatgaatcag actacacgtt tgcatctgca gataaatgaa caaaacgatc aaggaataca
    47701 attttctgat caaaacaaac ctttatcatt caaagtggaa aaatttggag aagattattt
    47761 gctgcaagca ttgaatgatt ttgatttttt ctttatgcat tatcagtggg gagttatcca
    47821 agggacattt tattctctga cgaaatatca acaaacggtt tatcaaactt ggaaacaatt
    47881 gattcgccga ttggagaaac cagaagtagt gttcaagaaa aaagaattac cgtctttgtt
    47941 taaagagatt atccctctgc tgtcagagat cggcgacgta gctattgccc ctgcagtaga
    48001 agaggaagta gcagatatcc ccgtcgaatt tattttcttt ttccgaaaag caaaaggaaa
    48061 aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc tattcgactg acccgaagca
    48121 tgaggtctcg acagaacagg cttttcaggt gttacgtgac acagtagaag agaaacgagt
    48181 acttgatcta ttcagaatgt atcgctacca gaaaaatgaa actggttatg aacgtccatt
    48241 gccaagcggg gaagaactat atgccttttt ccgcacagaa ctatctgtct tccgtcagtt
    48301 tggtgaagtc agacttggaa gaaaattgcg agagttgtat ttagatgcgc ataaacatca
    48361 accgaaaatc gaagtaaccg agaccggttc atggctcgat attcgattcg atgtgacagg
    48421 gatagaggag caagaaatcg atcatgtcct gcaaagcttg ctgagaaatg atgcttttta
    48481 tacattagaa aatggtcagg ttctttcttt tgattcagaa gaatttcaac aaacaagcca
    48541 agtgttgcaa caattacggg aatcgatacg tacagaagaa ggaacgatcc atgtacctaa
    48601 aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc aatgctactt ttagtgaatc
    48661 gttccaaacg atggtacaag atctcattca tccagaaaga taccaggcac agcttcccaa
    48721 aggattgaat gcaacgatga gagattatca aaagcaaggt tttcgatggt tgaagatgtt
    48781 aggtcattat cagtttggcg gtattttagc cgatgaaatg ggacttggga aaacactaca
    48841 gacaattgcc tttttacttt cggaaaaaga agaacggaaa agcttctctg ctttgatcgt
    48901 agcgcctgcg agcttaattt acaactggca ggcagaggtg agaaaattcg ccccttcttt
    48961 aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa gaattactgg ctaaagacac
    49021 agatatccga gtgacttctt atgccagctt aagacaggat ctagctaatt atcagtcgca
    49081 aaaaattgat tatttgattc ttgatgaggc acaaatggtc aaaaatagca gtacgaaaac
    49141 tgcacaagca ttgagagaat tagctgtacc tcagcggttt gcactgagtg ggacaccaat
    49201 cgagaataat ttagaagaat tgtggtcatt gtttgcaacg attatgccag gatttttccc
    49261 gactaaaaca aaattcaggg aacttgccgc agaagaaatt gcgcaaatga ttcgaccgtt
    49321 tattttacgg cgagataaac aaactgtgtt gaaagacttg ccggaaaaaa cagagatgaa
    49381 tctatattct gcacttacag aagaacaaaa aactgtttat ttagcttact tgcgtcaaat
    49441 gcgtgaagaa atcacatcaa tggattctga agcattcaag aaaaaccgga tcgggatact
    49501 tgctggtctg acaagactgc gacaaatttg ctgcgatcct cgtctgttta ttgaagatta
    49561 ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta ctggtagctg ccaaagagaa
    49621 caaacgaaga gttttgctgt tttctcaatt tacagggatg ctgactatct tgcaagaaga
    49681 gttagccgag ctagggatct cgacatttta tttaagaggc agtacgaaac cacaagatcg
    49741 gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat gtttttctga tttccttaaa
    49801 ggctggggga actggattga atctcacggg agcagatacg gtcatcttgt atgacctttg
    49861 gtggaatcct gccattgaag aacaagctgc tggacgtgca catcggatcg gacaaaaaaa
    49921 tgtggtagaa gtgtggcgta tgatctctga aggaacgatt gaggaacgca tggattcact
    49981 ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa ggtaatgaag aacagttgac
    50041 gaaactgact gaagaagaca tccgcatgat tttaagtgta ggagaaatcg aggagtaaga
    50101 gactcgaaaa agcgttcagt aatgggtaag aaagcctttc taaagaagcg attgtttatt
    50161 tgatttaaaa aatagcaagg aggctgggat tccattttat cccagcttcc ttgctatcat
    50221 ttttttattt ctatttttaa aggttataca aagattgtct tcattgtctc taacagctga
    50281 tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt caattttgcg ttctcgatgg
    50341 ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta gaacatcatt gtcgtaatta
    50401 tcgccaacgt ataaggtctc ttctggaagc atatgaaatt ctttctcagc cagctgaaaa
    50461 atctcttttt ctggtttttg gaaacctgtt gcttgagaaa tgagcatatt gcgggaagga
    50521 atccaattgt tcaattgaag ttgcttcagt ttttttgtct gatggtcagt cggcccattt
    50581 gtgataattc ctaatgggac attcttttcc ttcaagaaat cgagggtttt tttgacctcc
    50641 tcatgcaagc agatattatc aagttcttcc tcgtaaattt tttgaaataa gaggccatct
    50701 tcttccgtaa tatgagggta gtctaaatct tttagtgatt ggctgatccg atgtgctcgc
    50761 atatattcta gtgtccaatc tcctgccatt acttttggga agttttcatc actatgatgg
    50821 cggaaccgga tatatagtgg atgcatatct gcatctgaaa caagcggaac gactcttttg
    50881 accgcgtttc gaaatggctg ctgctgatca tacatcgtat catcaacatc aaacacaact
    50941 gatttaatca aaattttaca cgcctttcgg ttttcattga ttttttcaca aatccttgac
    51001 tattgtaaca gaaaaaattg tctaaaaaag ctgataaata aaggtttttc ttactatatt
    51061 tctatttcct tgacagaata aggtttttat atttcagttt ttttcactaa aaatcgtgaa
    51121 acacgtcaat aagtttgatg aacaaattaa tcattatggt agaagtcgat tgatttattt
    51181 taatgcaatc tctgtggaat tttcgaaaga tttatttttt taatacagtc tcaatata
//
LOCUS       DAYYFU010000015        48709 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000015 DAYYFU010000000
VERSION     DAYYFU010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.015"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACHA34_001040"
     CDS             33..656
                     /locus_tag="ACHA34_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791698.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACHA34_001041"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACHA34_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8791699.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACHA34_001042"
     CDS             2152..3033
                     /locus_tag="ACHA34_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8791700.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACHA34_001043"
     CDS             3036..3806
                     /locus_tag="ACHA34_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8791701.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACHA34_001044"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACHA34_001044"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFM8791702.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACHA34_001045"
     CDS             5117..6280
                     /locus_tag="ACHA34_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8791703.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACHA34_001046"
     CDS             6397..6807
                     /locus_tag="ACHA34_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8791704.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACHA34_001047"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACHA34_001047"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8791705.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ACHA34_001048"
     CDS             9380..9664
                     /locus_tag="ACHA34_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791706.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="ACHA34_001049"
     CDS             9824..10060
                     /locus_tag="ACHA34_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8791707.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="ACHA34_001050"
     CDS             10057..10455
                     /locus_tag="ACHA34_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8791708.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11122..12066)
                     /locus_tag="ACHA34_001051"
     CDS             complement(11122..12066)
                     /locus_tag="ACHA34_001051"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8791709.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12362..13306)
                     /locus_tag="ACHA34_001052"
     CDS             complement(12362..13306)
                     /locus_tag="ACHA34_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8791710.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13527..14741
                     /locus_tag="ACHA34_001053"
     CDS             13527..14741
                     /locus_tag="ACHA34_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8791711.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14763..16778
                     /locus_tag="ACHA34_001054"
     CDS             14763..16778
                     /locus_tag="ACHA34_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFM8791712.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16861..18234)
                     /locus_tag="ACHA34_001055"
     CDS             complement(16861..18234)
                     /locus_tag="ACHA34_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8791713.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18260..18544)
                     /locus_tag="ACHA34_001056"
     CDS             complement(18260..18544)
                     /locus_tag="ACHA34_001056"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8791714.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18546..20561)
                     /locus_tag="ACHA34_001057"
     CDS             complement(18546..20561)
                     /locus_tag="ACHA34_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8791715.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20740..21042)
                     /locus_tag="ACHA34_001058"
     CDS             complement(20740..21042)
                     /locus_tag="ACHA34_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFM8791716.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEVALAGTNSGETEDKEESLAGTN
                     SGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21131..22162)
                     /locus_tag="ACHA34_001059"
     CDS             complement(21131..22162)
                     /locus_tag="ACHA34_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8791717.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22369..23244
                     /locus_tag="ACHA34_001060"
     CDS             22369..23244
                     /locus_tag="ACHA34_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8791718.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23396..24238)
                     /locus_tag="ACHA34_001061"
     CDS             complement(23396..24238)
                     /locus_tag="ACHA34_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8791719.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24330..26207)
                     /locus_tag="ACHA34_001062"
     CDS             complement(24330..26207)
                     /locus_tag="ACHA34_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8791720.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26204..27625)
                     /locus_tag="ACHA34_001063"
     CDS             complement(26204..27625)
                     /locus_tag="ACHA34_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8791721.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27628..31017)
                     /locus_tag="ACHA34_001064"
     CDS             complement(27628..31017)
                     /locus_tag="ACHA34_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8791722.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31274..32665
                     /locus_tag="ACHA34_001065"
     CDS             31274..32665
                     /locus_tag="ACHA34_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8791723.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32738..33385)
                     /locus_tag="ACHA34_001066"
     CDS             complement(32738..33385)
                     /locus_tag="ACHA34_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8791724.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33393..34079)
                     /locus_tag="ACHA34_001067"
     CDS             complement(33393..34079)
                     /locus_tag="ACHA34_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8791725.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34085..35158)
                     /locus_tag="ACHA34_001068"
     CDS             complement(34085..35158)
                     /locus_tag="ACHA34_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8791726.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35380..36054
                     /locus_tag="ACHA34_001069"
     CDS             35380..36054
                     /locus_tag="ACHA34_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8791727.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            36059..37399
                     /locus_tag="ACHA34_001070"
     CDS             36059..37399
                     /locus_tag="ACHA34_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8791728.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37548..38534)
                     /locus_tag="ACHA34_001071"
     CDS             complement(37548..38534)
                     /locus_tag="ACHA34_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFM8791729.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            39258..40289
                     /locus_tag="ACHA34_001072"
     CDS             39258..40289
                     /locus_tag="ACHA34_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8791730.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            40398..41930
                     /locus_tag="ACHA34_001073"
     CDS             40398..41930
                     /locus_tag="ACHA34_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM8791731.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            42069..42881
                     /locus_tag="ACHA34_001074"
     CDS             42069..42881
                     /locus_tag="ACHA34_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFM8791732.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            42871..43356
                     /locus_tag="ACHA34_001075"
     CDS             42871..43356
                     /locus_tag="ACHA34_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8791733.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            43379..43924
                     /locus_tag="ACHA34_001076"
     CDS             43379..43924
                     /locus_tag="ACHA34_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFM8791734.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(43997..45640)
                     /locus_tag="ACHA34_001077"
     CDS             complement(43997..45640)
                     /locus_tag="ACHA34_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8791735.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(45769..47997)
                     /locus_tag="ACHA34_001078"
     CDS             complement(45769..47997)
                     /locus_tag="ACHA34_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8791736.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(48030..48509)
                     /locus_tag="ACHA34_001079"
     CDS             complement(48030..48509)
                     /locus_tag="ACHA34_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFM8791737.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        14829 a         8971 c         9170 g        15739 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg
    10621 aaatagtggt ttagaactag tgggctgatt ttttatatat tttagtttac ataatgtaca
    10681 ttatacaaag tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc
    10741 gtcatttttc catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt
    10801 actttttttg tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata
    10861 tgaacgaatt agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca
    10921 cagataaaca ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca
    10981 tatgtacgga tacattttcg gaccgattaa ttttaaccat tctgtttatt tgcttaccta
    11041 ttagatatca ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggattt
    11101 ttagttcata ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt
    11161 tgatttgctg aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac
    11221 accattttcg tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc
    11281 agttaaataa gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat
    11341 acgtgcagta ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata
    11401 agcagcatta cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc
    11461 tttttctaca atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac
    11521 ttctgaatca ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa
    11581 tttcaatgcg atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac
    11641 acgtgatgtt ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt
    11701 tgaagcaaca acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg
    11761 catgattgac gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat
    11821 accagctgtg ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac
    11881 agccacattt tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc
    11941 tttttcttga ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc
    12001 ataagcgata cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt
    12061 tttcatgaat aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct
    12121 tgtttattca ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa
    12181 acgaattcct gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat
    12241 aatacaacca aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa
    12301 taagacagca atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt
    12361 cttaatgttt atttctccat tcattgggag tacatgttga atacttcttg aataatttga
    12421 ataaagaagt cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg
    12481 tttcttgaat cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg
    12541 aagcatcaaa atacattttc aatttattac taagatagac cggattgtag ccaaaatgag
    12601 ctgctgtttc ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa
    12661 gcatctcatt ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag
    12721 tcaattcgat cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg
    12781 tatcaaaata aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc
    12841 cgcgaaaatg aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag
    12901 aagaaagtag attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt
    12961 cttttaataa gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag
    13021 cttctttatc aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa
    13081 tttgtaaaca tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg
    13141 gaacttccgc atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc
    13201 ctcggtggat ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca
    13261 ttccttgttc acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat
    13321 tcagctagtt ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca
    13381 cgctgtactg ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg
    13441 gacatgaact tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga
    13501 taagcgctta caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc
    13561 tgctgtcagt ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta
    13621 tgtcagctac ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct
    13681 cttttatggt cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa
    13741 aacaaactgt gatcatcgga ttagtgattg catcgatttg tggtatcctc cccctcttct
    13801 cacaaagtta cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga
    13861 tcaattcttt ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta
    13921 tgattggttt tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag
    13981 gacagctagt acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta
    14041 ttcttgtctt gtttgttttg tttgttccta cacctgaaac aactgaactt caatcgcatg
    14101 gcacagcccc aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt
    14161 ttctcttgct ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga
    14221 cgacttattc tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg
    14281 caagtatggc tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc
    14341 ttacactttc tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc
    14401 tgtatgtgat tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt
    14461 acttattcaa ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa
    14521 tcttattggt tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct
    14581 cttttgcttc tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt
    14641 tgattagcgc gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa
    14701 gagtagatga agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta
    14761 aaatgacaaa tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc
    14821 gccatggact gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact
    14881 ggtacatgga tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac
    14941 cttccatgtt taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac
    15001 atccatgtgg aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag
    15061 gacatacatc agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg
    15121 gctggacagc aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg
    15181 atatcgcaaa agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg
    15241 aaacggagat tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag
    15301 aactaatatc tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg
    15361 tagaagctat ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga
    15421 aagctcgtaa acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc
    15481 aacttcaagt ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt
    15541 cttctttact gaatcagatc tggtcagtat cagaagaagc cttcaagtta tgtagtgatt
    15601 tgttttttat cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat
    15661 cattcatgat caatcaatat cttttttttg acgaggaaat caataaacga acgatattag
    15721 cattaagagg acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct
    15781 tgtggttgat tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca
    15841 tcgtctaccc taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag
    15901 ggtttattta tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag
    15961 aaggaacgct tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt
    16021 gcggagatat cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc
    16081 aagaaaaata tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg
    16141 gcgcttttat cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt
    16201 tcgctgtttt attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct
    16261 tattgaatga cactatcaca cagattacta ctccatattt caaattctat gaacaagatg
    16321 ctttatgcaa actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag
    16381 ggatgttatc tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa
    16441 atgaaaagta cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag
    16501 caagcccaat ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt
    16561 atgaagaatt tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac
    16621 ctatcaaaga tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg
    16681 atagaacagg cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc
    16741 cacttgtctt aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat
    16801 atcagaagac tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc
    16861 ttaattctgt tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa
    16921 tacgacaagt aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc
    16981 tagtttccca agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc
    17041 gattttaaga tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct
    17101 attgaaagca gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc
    17161 agaagcagct ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa
    17221 gcctatcata gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa
    17281 tacagcatta ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg
    17341 tacgagtttt ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac
    17401 cccttgaagg acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat
    17461 tacagcgttg tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc
    17521 aagtgcgaca tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa
    17581 gctgaaactt ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt
    17641 tccgatccat ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt
    17701 gtttccagtg atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt
    17761 tccttcgaag accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac
    17821 taaaatgact gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa
    17881 tttatatctt gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa
    17941 agcagtaggt gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac
    18001 tactccttga gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact
    18061 gattaaacca gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg
    18121 gatcacttct gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac
    18181 gaggatggag ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag
    18241 ttcctccctt gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt
    18301 cttcgctgtc aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg
    18361 tatctttcaa agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa
    18421 catccgtatg acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt
    18481 tgatgttcat ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt
    18541 tcatatcatt cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt
    18601 aataaatctt gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga
    18661 agatgtgcct cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga
    18721 ttatcaagca gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg
    18781 ccatcttctg gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca
    18841 tactgctcca cgcgctcgat catagctttg atataacttt cttctatttt attttctgat
    18901 aatagcggct gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat
    18961 tggatcattt cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct
    19021 aaaagtttgt tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct
    19081 tttaattcga tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat
    19141 cctggtatca gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc
    19201 atgattggat gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta
    19261 gaaaaaataa tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc
    19321 tctgtaaaaa agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag
    19381 ctaatgccac ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc
    19441 gcttttctga tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg
    19501 ctcttcccta ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa
    19561 gcatattgat gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata
    19621 cgaaaatagg cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca
    19681 aattctacgg ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa
    19741 aaatcaagtg caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa
    19801 aatataatct ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta
    19861 atgtcagtga ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt
    19921 tttccaattc gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt
    19981 gcgaatcgaa gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa
    20041 taatcaatgg aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc
    20101 atcaaacgag cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc
    20161 ctaaaaccag ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt
    20221 atctttttga tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga
    20281 aaaacagaaa cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct
    20341 tgttcggtat acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt
    20401 gctcggaatt tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat
    20461 tgtttgatcg cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt
    20521 tctcttggtg ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac
    20581 ttatattcta gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg
    20641 atttttttta cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg
    20701 accaaatgtc tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt
    20761 ttttgaggtc agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt
    20821 ttcaaagtga ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa
    20881 agctgtttct ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc
    20941 ttctgtttca ccagaattag ttcctgctaa agccacttct tctgttttag gggccattga
    21001 acctcttaat cgtttgtgtt tttcaccgtc ataatttgtc atgttggttc ctcctagtag
    21061 ttctatactt tttcatcgta caataagaca gtttatgcgt caaaaaacaa acccgattct
    21121 tacaaatatg tcatattttt ccttctgatt cttgaatgct tttgattcct tcttccaaaa
    21181 ttttcatttg taagattgtt tcttcatctt tgatgacttt ttctgcgcca ttaactaatg
    21241 tctcgtaaat cccttcgtag acacggctgt aatcaccaac ctcggaaatt actttttctt
    21301 catgataact cccttgttca tctatatagg tcaatacacc ataatgttcg ggcaaatcga
    21361 taccaaagtc ttcatgatca ggcatgtaaa acattttcag atgttcttct tgtcgatctt
    21421 ttgtttgttt cacgaacatc cctttttttc cgtaaacgac gaaacttgga cgttctttga
    21481 tgcggaaata actggatttt atcgaaattt tcatatttcc atagaaaaaa tccagatcga
    21541 agtaatcatt cattcgtcct ttccctaaaa gttgacgtac atcatagctg atatcatctg
    21601 gttcgccgaa ataagaaaga acttgatcta ctgtatgaca agcatgtcca tataaatagc
    21661 tgttcgcagt agaaaattca tgcgtatttt ctggtacttc cggtctgaaa taatcataat
    21721 gcatttctac ttctaaaata tcccctaatt ttccgctttc gattactttt tggactgtca
    21781 agaagtcaga atcgaaccgt ctgttttggt aacattggat aaatagacct ttttcttttg
    21841 ctaatgcaaa taactcttct gcttcctttg acgtttctgc aaaaggtttc tctactagta
    21901 cattcttacc atgttcgagt gccatttttc catattcata atgaaattga ctaggtgtag
    21961 tgatgacaac taattggatc tcaggatcat cccaaagatc agacagctga tcagtatatt
    22021 cgatgtcttc gaatgttttc cacactggtt cagatgggga aaaaattttt ttgatattga
    22081 ttttgttcgt aagttttctt gaaaaaggca aatgatagcg atttgtactc tttccattgc
    22141 caagataagc gattgtaagc atgagtggtg cctcctttta ttgatgtttt cagtatatcc
    22201 tagaaaagcg cagaaagtat ttataatagc tattctggta aaaaagttca gaaaaacact
    22261 tgttatcatt cggtttggtg aaattaatgt tcaaagtaag gataaaaatc aaaaagatag
    22321 tagcggaacc aagtgtttat cagtaagata gaatttggag gtcagatcat gcttattgaa
    22381 gaaaaactaa caaaacaaga gttgtttacc acgacagaga aacggattgt cgattacatc
    22441 cgtaggaata tcgaagctgc agtctacatg acgattgaag aattagcaaa agctacgtat
    22501 acttcccatt cagctatcat tcgtttatgc aaaaaaatgg gattcagcgg atttaaagaa
    22561 ttccgtttcg agctggggcg ggaagttcat caattgattg ctcagttcaa tcaaacgatt
    22621 gatcctaatt ttcctttttc aagtgaagat agcactcagc aaattgcaaa gaaaatggcg
    22681 gatttatcga tccagtcaat aaaaaaagca caattgcaga ttgaacatga agatttagat
    22741 caaatcggta aagtattgaa caaggcacag cgaatctttc tttttgcaaa aggtgattct
    22801 caaatcacgg caaggaaatt ccagaataaa atggtcaagt tgaataagtt tttgatcatg
    22861 gctgaagaat acagtgattc ttcttggaat gcagccaatt tgactagcga ggattgcgcc
    22921 atttttatta gctacagcgg tcgcatccat cattatgaac gaataatgac ctatttgaag
    22981 catgtagagg cccctacctt gctgattacc ggaaatcagc attcagaaat ggccaaacag
    23041 gctgatatgt gtcttgttat atcgcaggag gaatatgatt ttgctaaagt agcgactttc
    23101 tcctctcaaa ttgccttcga ttacgtatta aatacgttat tctcagtgat atattctcaa
    23161 aattttgaag agaatatccg caacttgaaa aacaaacaag aattgatgca aaaaggcctt
    23221 cttaaagaat atcggaaaac ttaagaaaag atcctcattt agtaataaga aagatagtgt
    23281 ttgataggct ctctttgata tcatcataaa aacaggctga agttttcttt ttcaaacctc
    23341 agcctgtttg tttacggaac gattcgaata gatatattat agaaatgagg aatagctaac
    23401 ttctgtttct tattttgaac gattgacgaa aaagatatat aagaaggagg aaaatcagta
    23461 tacatgcagc taacaatcca tacaggaaaa atcgtttgtt ctcggatact ttctgtactt
    23521 ctttttgaac ttcttttgca tgataaggaa cccggtgccc tctgacaagt aagcgatgac
    23581 tgttgaccat ataaggtgta caagttagga gtgtcacgaa atcctgccct ttttcgattc
    23641 tcagatcttt tgtatcagtc ggttctaccg tcttgatctg atcgacttga taagcgagtg
    23701 tccgtccatt gatttcgata tagaaagaat cacctaaagt cagtttcggt aagtctgtga
    23761 acaactttgc ttgaggcagt cctcgatggc tggaaataac ggcatgtgta ttttctcccc
    23821 ccgtaggata agacgttcct tccaataatg aggcaccttt ttctagcagc acagtattcg
    23881 tttcatcaaa aattggcaaa tggacattga tctcagggat cgtcaaaata ccgatcgtat
    23941 gtttctcaaa ataggttcta tcctttttcg caggttcttt tttcttagta aagggatctg
    24001 cacctggatt tccaccttct tttgctaatt gctggttttt ctttgtcatg ttttcttgga
    24061 ttttagccat gacttcttca ttttctttga ccgcttgttg ctgataatga gagatgatct
    24121 gctgatccag ataattattc agcgcgtcgc taacaaatgg ataagctagt atccctattc
    24181 ctgagagaag caagaaaagc atcagcagat ctagaagttt ccttttcttt ggtttcattt
    24241 gtgttctcct tgtgatgact gtttgataat aaaagaaaaa ggagcatttg cgtaatccgc
    24301 tgctcctttt tccaattctt gcaaaaagac taaatctgac tgtgcttgcg tctagcaaag
    24361 tacagtccag caatcagtag aaggactact cctgcaccga tatacacata gattccctta
    24421 ccgcctgttg aaggaagtgt acctttgtgt ttatttggta ttttttcagg agaaatcaac
    24481 tgtcctgctg ttacataaga ttgttcatcg atagtaaatg ctacacggtt tgttaatgga
    24541 acatattttt ctggcgcttt cgtttcttcc agatagtacg tgccatattt tagacctgtc
    24601 acatcgatta aaccgttact tgtggttgta aaaaccgttg ctgattcttt cgccgatacc
    24661 cagctgacgg cttttgtgga tgggtcgatc gataaatatt tcgctgtgtc actatcttga
    24721 tcacgaacga cgaattctgc tccagcaagt gtttggtctg atgtaacgtc accatctact
    24781 ttaacaaatc gtttgccccc agtaacgaca tcgactgacg gtggtgtttg atcatttgta
    24841 tgcccgttat cgacatttgc ttggttagaa aatcctttgg ttggatctgc tttttcgttc
    24901 aaatgcatat agtaaacgaa tttcaatgta ccgccaggag ttaatgaagg aatataattc
    24961 ggatcaactg aaaccgtgaa tccgtctgtt tgctcagtga cagaataatt tactgggtcg
    25021 atttctttat ttccatcata taaggcataa gcatacgttc ctgaagaatc attatcaaat
    25081 gttaaagcag cgtcatgagt atcgataagt ttgaatgttg tgtacttgtt ttgcgttcct
    25141 tctttatcag cgatacctaa tgggatattg acagaaatct catattgaat tttttcaccg
    25201 attgcgacat cttttccatt caattgaggt gttgttttat caactttaga cgtatcattt
    25261 ttgatggtct tttctactgg tgtttggcta tttgccagga tctcaaaagg cgtgattgtt
    25321 tgcttttcaa tcatttccgc attatcagga gcttttactt cttctaaatt atattttcca
    25381 acttctaaat gattaacgat caactggcct ttttcaatag atgcctcggc aaagtcattg
    25441 ttgccgatgt cataagaatg accagtaatg aaatgtttgg ctttggtttg atcagttgtc
    25501 caagtgtaca atccatctgt gacactttgg atgtattttt tgacgcttgg tgttccttct
    25561 tctttagaaa taataaattc tgctccattt agtgcttcgt tttcggcagt accgattttt
    25621 gtaactttca acgttccatc attaccgact gtatttttag ggtagagatg gatagtatct
    25681 agttcttctg tcccgtattt ataagagcca tctgcttgtt tgatcatctc atatacaggg
    25741 aaagctaaaa ccatgtttgc ggcagctgac actccgtctt ttggttcttc tttgatcgtg
    25801 tagactgcat ctttcccatt ttgtttttta ggtaaagata aagtgacatt tccatcagca
    25861 tctgtcgttc ctgaagcaac tggtgtacca ggagtcaaag actggactgc ttgttttgct
    25921 gcatccacgg acgctccttt atctcgttgc gcataaaatt cttgagtgac gttataaact
    25981 gaaaatgaaa tatcggctaa tccttggtat tgatcgaatt cgctcatttc tttcccgctg
    26041 ttttggatta aaggatcggg taaatcagtc attttctttt tatgaaggat gacttgagct
    26101 gctgtctcct ctgcaaacac tttttttgcg ccgaagctgt ttgtgagtaa tggtaaaata
    26161 aggaaaagga ctcctaacat aacgtttatt tttttatgat ttttcatttt ttcactcctg
    26221 ttcttttttt gataaacaac cagattgcaa agcttgcgaa taccatacca acaacgccta
    26281 aaagtgtatt tttcgtttcg tttgtttttg gaagaaatgg acggctattt tcttttttat
    26341 tgactacttt caatatactg tccgcctctt tatcctcaga aagaaaagtt gaaatggtaa
    26401 acggaatttc tgtttcactt aaaatataac cagaaggtgc ttcgatctct tttaatcgat
    26461 aacttcctgt tttcaacccg gaaattgaaa aagcgccatt tttatcggaa atcaggtgaa
    26521 gcgcatctga ttcgttcttt gtccacttgt atccgttggc tgtttgctgg aggtaatttc
    26581 ctgctttgtt tttaataagg aatactgcgc cagctaatcg tttatctggt ttatttgtct
    26641 cgattttgac aaattgctta ctccctgtat atacagagac tttctttgta ctgacaccaa
    26701 aaccaaaatc cagtgttccg ttgttgttga tttccttgtt cgcctgtgct gtactgctta
    26761 gctgcatctg ataagaaatg gtcagttttt ttccagcata tttttccaac gaagggatcg
    26821 aaaataaaac atcaaatccg tgaggtttct tgtgtatgac aaagccttct gtcagctttt
    26881 ctccattcga tgaaatcgtt aaactattag gtaaaaacgt taatgcttca tccgcactat
    26941 ctgacaattc gaacttttga tagtcaagga tatttacagg aattgtcgta tgcaaggaat
    27001 aagtgatttt ttcaccaatc gtaaaatcat ttctaggctc ttctaatacc ttctcaaaag
    27061 gtggatcagg gtagtcgttt tcttcatttt taggatacaa atggatagtt gaaagttttt
    27121 gattgttttg tccatgtaca ggaagaacaa ctaccatatt ctctgccttt tctttgacga
    27181 cttctggtac tttggattca atgaaaagat acgctttatc tcttttttct tgatctttag
    27241 cggccagaga aaaagaagcg ataccatctt cgttgttaag agttgtagtt gttgcttctg
    27301 cggtaaacat accggaagac gcaccgtttt ttgcgatctc tgcttgtgct tcttctaccg
    27361 ttttgccctt ttcccgtaga tggtaaaaag aatctgtgac atcataaact tggaatgtga
    27421 caccatttaa tcctcgatac gtttgtaata aagctttttc ttgtccgtca tttggatgat
    27481 ttttcggcag ttgcccgttt gggaaaagca atttgtgtaa agtaatctga accatttctg
    27541 tttctgtttc tgttgctacc tgagtaaaag ctggtttaaa tagtaacaag aagagacaca
    27601 tactaagcca accaagtttt ttcatattta gctgccccct ctttcttttt tcaaaaaaag
    27661 ataccaaata gaaaacgcac aaccaatcat ccctactaga tagattccta aacggccaat
    27721 tcctcccgtt tcaggtaatg gtacttttgc ctgattcgtg atgtctaaag aaatctggtt
    27781 gtttttggct cctggtgaca gaacagattc atgatcttgt ccatccactt gaattgaccc
    27841 atcttcgtgt ataactatag taactggctc ttttagacct tggtatcctt ctggtgtaaa
    27901 agtttcggtc agcgtataag ttccaggttt taaattttcg aatagaaagg tatctgtttc
    27961 ttgtccatct tttggcgatt ctaagtccat ttctggtccc tgcagtctga attttgctcc
    28021 ttttaatgtc tgtccgttgt cttctttttt gtgtaccgtt agatcaaacg cttttagtcg
    28081 attttgatga gtgagcgtcc atacattatt tgtcatctcg gtattttttc ccgtgtaaat
    28141 gatttttcca tatttatcga tttggaattc ataatttccc ggcgaagtat catatccggc
    28201 aggaccagct gtttcaacga ctcgatattt accaggtgtg agtgaatcaa aactggcaag
    28261 acctttttca ttcgttgttt ggctgtcaat tggctgataa gtaccatttg cttctttttt
    28321 ctgtagggaa aatgctgctc ctttaagatt tatctctttg tccgtccctt gcatcgcata
    28381 cttacgtact gctacaggaa cttcaacaaa aggattttca atagtcaact ggatcgtatc
    28441 atcggaagta gtgactgttt ttccatcaat ggttaccgta ccatcagaag cgatcttgat
    28501 ttcccaagta gtctttttgc ttaatccatg cccttctgga gcttttgttt ctgtcagcgt
    28561 ataggtcatt tctttttgca atttgacatt ttctggaaga gaataggttc cgtcgccatg
    28621 atctgttagt aaagtatcaa tagaatctcc tgttaatttg aaaacagcgc caataagatc
    28681 cttttcacct gtagaggaat ttttcgttat tttcaagttt aacggtgtga attgtttagt
    28741 atttttccat gtcattgcat ctatttgttg agaatcgtat cctggtacag gtaattcttt
    28801 gattgtttgg taactgaatg cttgaccttg attattgtat tgaggtagtg ataatatctc
    28861 ttgataactt tctccgctat ttgcagataa tttgtcaatg tctgcacgtt cccacgtatt
    28921 tgttgtatct ttagctggtt taatgattcg aatatatccg tttttccaag ctgcagcatt
    28981 tgtcgtatgt tcccgttgaa tctcaaaagt aacttgatct ggacgatcag ctagattgtt
    29041 gtcaaattct tcccataact ttttgatgtg aagactgact ccgggagctt tagcagatgg
    29101 tataccgaat tcagctaatt cattggtatc aatgtttggc tggaaagttg tcctgccgtt
    29161 catttgatac cagaaatttg gatggaagtc ctcattttct gtttggattc tcacttggta
    29221 atggatttgg atttcttggt cttttcctaa atagatctga ttgctcttga tggtatttcc
    29281 ttctatggaa atagatggag tgaccgttgt aggacttgtc cccacactct tgactgaaag
    29341 tgtaccaggc tgataaatga atggttctgc gattggatca tttatttgtc cattgcttac
    29401 agttgctgag atctgtacag cttttttggc taggtattca gagatatctg ttgcatgatc
    29461 agctgattca tagtaaagat cgcctttttc atctgatgaa accatttgtc gcatacgaga
    29521 ctctacttct gcttttgaga gaccagctgc cggatcgctt tgaagttgga tgccaagacc
    29581 atgtatttcg attcctcgtt ctttgagtgc catcgcttct ccgatggttg cgataaacgt
    29641 actgtcgatt cttctggata gattgttttg gtcaggtgca tcatagattc ttgagattag
    29701 agaagtattt cccggccgat cttgcgtatt agaaaactga gttccgtaat aattgctgct
    29761 tgattgtgcg tgtacccgct gtactttata ggaaaatgtt ggtacaccat ccgtcagcaa
    29821 aacgatcact tttttatgtc catttggaac ggatagcatg cttcctgcat ctcttagtgc
    29881 tttttgtgta aaagtaccac catttgtccg tgaaggtgta atggatttta cttgattttt
    29941 cactgaatca aatgaaccca tctgtactgc accgttactg tagctatatc cttcgcttga
    30001 gtagccgaca tatcccatat tgattttgtc tgtgataccg ctatctgcta aagtatcgac
    30061 aaaacgatcg acaccaatct ttacttcacc gatccgatta ttgtcgttca tacttcctga
    30121 ccagtctacg accaatacga gatcaagagg cgtgatatct ttttgtacat ttccacgagc
    30181 attcaaataa acatcaaaca atccgggtgt acttgtttcc tttgcatact ttcgtatcgc
    30241 atagtctgct tgattaccgg ttcctccata ttcgatatac gaatgggtcc gatcattagg
    30301 atctccattc caagaattta taccatccca ttgatttgtt ccgtttttat tgccttgatg
    30361 atcaagaaca tttgtatttc ctgtaggttg ccaactggct ttaggataag tgccctgatc
    30421 atctgttgta tattctgtag gaatcgtttg attagatggc acactcacag ctggcaagga
    30481 taatgtaggc aatgtaaatg ggctgacaga attttcgttt tcttttgatg attcttcttc
    30541 tatttcgtct ggctttggta ctgacacatc tgtatctttc ttattttctg tctcatcaat
    30601 aatgtcatat tctttattat cgacgatcag cttgtttgcc tggctgtggt aaagtgctgg
    30661 atcgattgga aaactcagat cgatagtttg tttagtgttt gatgagattt ccaacgtaat
    30721 tttcccgcta tttacctgat aagcatagcc atctccttct tttctttcca catcacttaa
    30781 gtaattttcg actagaccaa tttctttttc tatcttttca gcactggtgt tattcgcatt
    30841 tagcttgatg ttcagtgctt tttttccaga ctgatccgaa atgacttcag ctgaaattgt
    30901 ttctaaaact gttggatcat ctgtagtgtc tgctattgca gataatggca caaagtttga
    30961 taaactcaat attatcagca tgaaaataac tttcagtttc ttccctgtgg tcgtcatttt
    31021 tcccctcctt tttctttttt tctgttttga cgtgaatagt gaaaattatt aaaataaaca
    31081 ggcgttacat aattttattt attttaatat gataattatt atacgaaggg cgctaaaggg
    31141 aatcacacca attttcatac caattggttt catttatgaa gttgattcct tcgattaatg
    31201 agcgtttcat tgaaagaaaa gggttgaggg agaaaaacat atgatcaggt tgttggaagg
    31261 aaactataga aagatggaaa aaatcattcg aattctgtcc atttctgaca agtggatgac
    31321 taaaaaagaa cttgccaaac gaattggtag ttcagaatct acatttgtaa ggtatatcga
    31381 agagatcaaa atccgctggg gaacggatgt aacgattcaa tcttcacaaa aattggggta
    31441 tcgtcttgaa catttgaatg taccagctta tttaaatgtt ctgatggaaa tgtcgcaaag
    31501 ttctacaaac agtcgtttat taaatgagat tattgtcaat ccgggtaaaa cgattgagta
    31561 ttattgtgaa aaaatttcca tcagccggtc ttcttttaca cgtaagttga aacaatgcaa
    31621 tcaaatttta gaaagctatt tattaaaagt gatcgttgac caaggttttc agttaactag
    31681 ccttaaaagt gagctccagt tgcgtatctt tgttaccttt ttctttttaa gttactacgg
    31741 aagacgcacg ttgccatttc caatcgaaaa aaagaagatc aaagaaatta tggtcaagaa
    31801 taattgtcag atgaatttgt ttcaggaaga aagcccatat gagcaatctt tctttattat
    31861 gtattttgtt gtgcatctca tgagagaatc ccaaggaact ccatttgaat ggaaagctgg
    31921 agactcttat gtacagttga taaatatcga taaaaaagac ttcttaatct tgaaaaatct
    31981 cttaggaaaa ttgaagtaca gcagctatat aaaagtagtg aactattttg tcaatacgat
    32041 tttttcagaa ttttcggaga aagaaaaaaa tgaaatcagt gagcagatac gaaaaaatat
    32101 gtcctactgt tcgttttcat ccgctgattg caaagaaaaa aacgaagaat atgttgttca
    32161 attattaacg caaatttatt ttttctcccg tattttccca tacgatacga ctcatttgac
    32221 gatccgtgca acttactttg cacagcagat gcaacaccgc cagatacgtc tatatgctgg
    32281 atttcgtgca gaacttcaat tttattccac tctactgaat cagaatctga tttattattt
    32341 atctagctat atcttttgga tgcttatcgg taatcctgaa attcaccatt atacttctga
    32401 taaaaaaata ctgattatca gcgacttaag tcttcgacat agtcaatata ttgaagaata
    32461 tatcagtgat attcttgctg tgcataaaat tcactctgag acaactgcta ttactgaaga
    32521 tcagctgtcg aaatacaatc ttctcgagta tgatctgatc gttacgaatc aacccatttt
    32581 gaatgcccat gttcctcata tattgattga tgatagtgtg tcattcgcaa atgaagaaga
    32641 actcatccgc ttattcgagt tatgaataat caaaaaacag ctatgtataa accaccctga
    32701 taaaaatttc aggacaaata cacatagctg tttcttttta ttgtattcct tcagcaatcc
    32761 aacgagattt atagcgaaag tcttttaaca actcttcttt tgtccaatta tttgcaatga
    32821 gatccgtcaa agtgttcatt acgagggtca tggaaaagta taggaaacta actgcttctt
    32881 ccgacgattg aatggagaaa tgattgattc ttaataattc cagccatttt tcgttcattg
    32941 gagaatcagc cgatagttct ctacgtttgg ttgtctgctg atcatttcgc tgggttaaca
    33001 agtataacct ttgccaatag atactttcgt gatctaagtc gcagcaccac tctaaatatt
    33061 tttcgatccc caaaaagaaa tgttgtttgt tttcgctaat gaattttaag atatcttgat
    33121 ggactatgtg agaactgctt ttgatcgtat acaaataagc atcttccaaa tcttgaaaat
    33181 atttataaaa agcaccgcgc gacatcccca tcttgtcaac gatttctgag actttgactt
    33241 ggctgatgtg ctgtgagcaa aatatctcca gtaataggtc atcgattctc tgtttctttg
    33301 cgttcgataa attgaaaaaa gtttcttttg gcatccacat ctctccttta tctctgatta
    33361 tacgtggata gcccttcatt gtcatgaagg attcattcct tctttttctc ttcttctgtc
    33421 aagtacccat cttccattcg ataaacgcga tcactccatt gaatcatacg gacatcatga
    33481 gtaaccataa tagtcgcttt ttgtttctca tgagcttctt taactagtaa tttgacgact
    33541 tcatatgcat gatctgtatc caaactagct gttggttcgt ctgctaagat caagcttggc
    33601 tcattatata aagcgcgagc aattgccact ctctgtcttt ctccaccaga aaggtcttta
    33661 ggataactat ttttaagtga ctggatatct aaagattgca gcaaggaatc gatttttgtt
    33721 gaatcttctt tttttgaagt aacttggtca actagttcaa attgttcctt taccgttaaa
    33781 aaaggaatca aattagagct ttggagaata aatcctattt ctttaaagcg taaatctgct
    33841 cgtttttttt ctggaagctc agttagtgag tgaccgttga tcactatttc tccggaagtc
    33901 ggtgtttgca atcctcctga aatggtaagg aaagtacttt tacctgaacc agaaggacca
    33961 atgatgctga cgaattctcc ttcctttaca taaaaatcaa ttgctttcaa agctgagacc
    34021 tttgtatgac cagaaccaaa tgttttggta acttttttca tcgttaatat ttttttcatt
    34081 tttattaacc tcctattgct gtaattgggt ctactttggt tatcgtacgt atcgaaaaca
    34141 atccgcctag cattgcgaca atgatcaaaa tcccactata tagcaaccat tgtgaccaaa
    34201 aaacagcaaa aggcatcgcg cttggtaaaa tcagactagt caaataagca aacaaaagag
    34261 caagcagaac gcctaccacc ccaacaataa aggactgtgc tactaaagat ttagcgatga
    34321 agaaattttt gatcccttgt gctttcatta ctccaaaaat agcagtcttc tgaagtgtca
    34381 tcacgtacat gaaaataccg accacggcag caaccactaa gaacaaaaag tatatcatcg
    34441 catttaaggt catattttgg gcagaatagc caggaatact ttcaatgaat gtcccaatag
    34501 ttaataactg tagacccttc gatgcttttt ctttcgaaat tttagctgaa tcctttatta
    34561 cgatcccatt gatcggctgt tcttcttcag aagcaaacgg ctgttgtcca tacttcaaag
    34621 cagtccacgt atctagggac gcatagatga cgggagaaac tgtgtaataa gtttcaggaa
    34681 agataccgac aatcgtcaaa ggatcgtcgt aactgccaat tttgatcgta tctccaattt
    34741 tatagcctga atcagccaga ttttgagaga tgatgatttc attctttgtt ttaaattcat
    34801 gccctttcgt gagttttggt aataaaaatg cttctttcgt tgtgccgaaa actgtaacat
    34861 tttctttgtt ttttcctttg attgctccgc tgtacaatcc gattggtgct ttctctcctc
    34921 cttcaatgta tttcagatct cctcttgtta attgtgaagc tgcaaatact tgatttgcat
    34981 cctccgacaa aacaatctct tgtgcgtccc aatcatcgat tgctttttta aactcttctg
    35041 aaagtccatt cgctagtcct gacaacatga atacgacata cgcaatcaag aacataatac
    35101 cgacgatcaa gccatatcgt aatttagaat agcgaatttc tttgatagct agaaacattt
    35161 gtttttcctc cctatgatag attgatagtg acaaaatgtc acttgttgaa tagtgtaact
    35221 ctaaagtgac aatttgtcac ttgtgttgtt tttttcttat caataaattg tctaagacat
    35281 ctttcgataa aaccttaaca tgagtcataa aaaatttatt taagccttat ttatgctttc
    35341 catgataaac taaagtgacc aaatcaatga ggtgggaaaa tgattaaact attagtagta
    35401 gaagacgata aagtattatc caataatata aaagaaatac tcgctgcaat cggcgagata
    35461 acacaagttt atgatggtag tgaaggactg ttcgagttgg aaagtggagt ttatgatctt
    35521 gcaatcttgg atttgatgat gccggaaatg aatggatatg aagtgttagc aaaaatacgg
    35581 aaagaaaaaa tacagactcc agtattgatt ttgactgcaa aagatagttt ggaagataag
    35641 attgaaggat ttgaacgagg tgcagatgat tatctaacaa aacctttcta tcgagaagaa
    35701 ctgatcatgc gcgtgaaagc attattgaaa cgctcgctag gtctctttga tgaaaatgtt
    35761 ctgaagtata aggatatcag ttgtgattta aaaagcaatg aagtcttgta taaagaacaa
    35821 gttttgccca tacaaggaaa agagtttgaa ttgctcgttt attttttaca aaataaggga
    35881 atcattttga caaaagagca gatatttgac cggatctggg gatttgattc tgatacgacg
    35941 attacagtta ttgaagttta tatgagcaac ttacgaaaac atctgcgaag tactggattg
    36001 gataaagaaa tcaaaacctt acgtaatgtc ggttatatac tgcaaagggt gtgatgcaat
    36061 gaatggagat attacacgaa agcaacgtag tcgctttttt gcgacaaatg tagcagcatt
    36121 tgcttttatc ttttttcttt tgggattcat tactttacag atattaaacc aatctgcata
    36181 cagagaaaca gatctgtcat tagagaaaat aaattttgat tctcgaacag tacaattaga
    36241 gatacaaaga tatcagcaag gcgatccctt tttaaacccg actcaaacgg aacaaaacag
    36301 aacaaacatg gaacagccat taatggaacc atcaaatgac cgttttaaca ctcaagtgat
    36361 cttgtggtct aaaaatggtg agatattgaa taaagcagct attggtgggc gtttcaacca
    36421 aatcaaagaa ttaaaaatgg atacgaaaaa tctgaatacc atccaaaata ttgaattaaa
    36481 tgaagacagt ggctcatctg ccctctcttt ccgttcgatt acgaaaaaag cggagacgaa
    36541 tcaagatggc gttgcctaca tccaaatttt agaaaataca aatcaaatca agaattcatt
    36601 agcgactttt cgcacgatta tgattttgtg tatgattgtt ttttggattc tctctattgg
    36661 tatcagttat tacttgtcta gtctaagcat gcgccctatt attctttctt ggagaagaca
    36721 aaaagaattt gttgagaatg cttctcatga attgcgcaca ccgttaacga ttattcaaaa
    36781 tagtttggaa catcttttta ccaagcctaa tcatactatt attgaagaat ccgaaagcat
    36841 cgctcaagca ttgtcagaga cgaggcgcct cactggattg acttcggatt tgctgacaat
    36901 cgctcggaat gattctgatc agcagctgct ttcaaagcaa atgatcaata cacaagaata
    36961 tatcgaacaa cttgttaaac catttcaaga gatggccatc atagatggaa aagagtttat
    37021 tctccataac gatgcaacat tagaagtacc aattgatgaa aaaaaaatcc atcaagtttt
    37081 ggttattttg ctggataatg cattaaaata tacgaatcca gaagataaga tcattttgga
    37141 ttcatctgtt aaccataaag aatggctgat tcacgtaaag aatactggcc cttctatcag
    37201 tgaagaagat caagcaagga tttttgatcg attttatcgg gaagatcaat ctcgggcgaa
    37261 agagactggc ggatacgggc ttggcttggc aattgcaaaa caggtaatcg aacagcatca
    37321 tggacagatt tctgtatcca attatcttcc acaaggagtc gattttaaag tccaattgcc
    37381 gttgaagcct aaaaaataat gcttatccct aaaaaagggc tgtgacaaaa atcttgtcac
    37441 agcccttttt tgttgcgaat aaacggtgtt ccagaagtag cttcttcgga aataagccga
    37501 aatttcttta agctgaccac ttctgtcaca acctctttag cttacgctta cgatttttta
    37561 ttgcggtctt caatgtgtac atcaccgctt gtgtcaatat ccaattcttc tcggctaaca
    37621 tcttctgtca ctttttcaac atcttcatgt tcttctttat gaatcccaac ttcttcacga
    37681 ataactgtat gtttgcttac atctacttgc tcttctttga ttgggatagt aatcgtgtca
    37741 tcttccaatt tttcgtctgt tccctgtgaa gattgatctg ttactggttt gcgttcgatg
    37801 acgatttctt cacgtttgac cggtacttct accgtttcag tatcgttgac cacatgtttg
    37861 tggatgtcga cttctccagt tgtcacatca tgtgtattta catctaattt ttcttctttt
    37921 agttgaattg tatcatcatc tagatttggt ttgcgatcga tgtcttcagt cttgcttgta
    37981 cccattgtat cagtctcgcc aggtacgtcc atcggatcag ttcctggtgt catattcaat
    38041 ggatccgttg tattttgaaa gcctgtcgtg ccacctacgt ccattggatc tgttccagtg
    38101 ccagtttcta atccagtccc cattgtgctt gctgggctaa cagtatcttg tgtatccatt
    38161 gtatcttctg ttgtttctgg ctgacgatag ccttttactg tgatgacaaa ttttccatca
    38221 gaaatatctc ctctatattg atataaagga tcattttctg gtgtagccgt ctcagaatca
    38281 taggtatcaa aagaaaaagt atcttttatt ttttcccata aagaacggtc ttcttcacca
    38341 tccatccgat tttcatctgt ttcaacatct ataccagaca atccgtcaaa tcctagtcta
    38401 gatgctgctt catcagtagt ataaataacg atatcatctc gttcataccc ttcgttacgc
    38461 aaccgttcaa ttactgataa tgcttcttcc ctcgtactat agcttccgat gaccgttaaa
    38521 tttttctctt ccatgtaaat tcctcctagc aaatagattt atgattggta tattgttact
    38581 tataaattta ttctatagga gacgattgtt ttctacaaat atttagattg gaatttgatt
    38641 aaaacaattt aattgaaatt tgaaaacatt tatgttaatg aaagtcatat tctaataaaa
    38701 tgtcatttgt ttctatatga atttttatga aaaatttcat ataatttcca ctcgttgtga
    38761 tggtttaatt tatccaatcc atcaataagc aaacaactta tataaaaaag gattaattct
    38821 caatataatt gctcctacca tagaataaat cttcatgtaa agtaatgaag taccaactat
    38881 ttctaatact tctttaagaa ttatcccaac aatatcgtta gacaaaccat tattgaataa
    38941 atggagagcc aatcgattct gaaaaaatgt tttctaattt ttcggaaata aaagaaagag
    39001 cctcctgcca ttagaacgaa ccaaatccat ttcattgctc atgaagtaac cacatagcta
    39061 aaaagaggat aaatcaatta aaagatcaaa agaatgactg cactaataaa ataactaaaa
    39121 atgtatcttc ttacttcata tcttttagct agtacttagt taaaaatttg cttcctaaga
    39181 tcaagacaaa ctgtctatct tcatttgatg aaatgcggta aaatatagaa aatgaacact
    39241 attggaggga atttgtaatg gacagcaaaa ctagaaaaca actgcaagaa aagttaaagc
    39301 aacgtacaca cttgctccct tccgtctttc aacagttttt agaagaaaat cgttcttccc
    39361 tttcattagg cagtcgctat gaatacgcaa aagattttca tctgtttact gattatttga
    39421 gaaaagtatt ttctgaagaa gtggatgatc agctgattat ccatttagaa aaacaagatt
    39481 atcagaattt cttagctaaa atctatcgac atactcgttc gttccaaact acagcgaacc
    39541 aagaaacgga gcaattgttt gaaaatagct attacgggat ttcaaggaag caatattcat
    39601 taaatcattt tttacgattt ttgtatcaaa aaggactgat tgaagaagag atttctcttt
    39661 tgggtacaag tttaccggaa acaaccaaag cagctccgcg atatatcgat gcagaacttt
    39721 tcaaagattt tgagcatttg tttatcccga ttgaaggctt tcaaacaagc agagaagcta
    39781 gctttgaaga aaagaataga ccgaggaacc ttgcaattac tgccattctt ctgtattgcg
    39841 gattaaagat ccatgaagta gtagaactta aacgtaaaga tgtatctttg accaagcaga
    39901 ttcttaatct gaatagaaaa gaaaacagac tgaataaagt accaatacca aaagaagcga
    39961 tgccatttct ggagaactat tttaagttaa cagcacaaaa tacggacgaa aattcttttg
    40021 tttttcgttc ctcacatgat cagcaaattt ctcctcggac tattcgccaa attttgagca
    40081 aaattacagt atataaaaac gtatcgccag aactatgcag aaaaacatat cgttattatg
    40141 cagaactata tttagatgat gctgacgcgg taaattattt atgtggtaat cgatacctct
    40201 catcccattc ttttcaagat atctgggaaa agatggttga tttttcttat cacgaattag
    40261 cggcattcgt tcgggtttct tccatataaa acaaatgtat gatctttttt tcacaataaa
    40321 ctatgttatt atataaagga ttatgttacg attatcttga atttgtatat tacttaacaa
    40381 aagataatgg agtgtgaatg atggagacca taacgtattc ccctttgaac ttatttacga
    40441 attttcaaga agctgcagct aacttttccg agaccccgat tatctttgat gagccacttt
    40501 cagctttccc agaattagga cttgaaacaa cttacaaaaa atgctgcgaa agtgtgttga
    40561 aacgggctta tcaattggct catcttggag tgaaatccgg agacaaagtt attatttata
    40621 agagttccgc ttttgacaca taccttctag ctgtttctgt gtcttacctt ggtgcagtgc
    40681 ctgtcatgac ttcttatcat ctaccaacaa caacaatgga agtatttatc gatcgtttag
    40741 aagacccttt cattttattt gatgatgaga caaaaaagcg agtgagagaa atttcaaatg
    40801 gaacaaaatc aaaacagatt cctattcttt atttaatgga acaacctgct ttgtctgttt
    40861 ctcaatcatt tttagacaaa aatgaaattt cttatatgac ccatacttct ggtacaactg
    40921 gtatccctaa acttatttgt cattctgctc attctatggg atggcggacg aaatggcaaa
    40981 aaacagtttt cacaaaaatt tcggaaaaga aattaatcgc ttttcacatc tctccagtgc
    41041 attccagatt caatattggt atctcttctt tgatgtccat gggatttcct atgatgcctt
    41101 tagcaaacgc acaaagttca aaagttgttc atatgttgga agctcaccgg ccaattgcac
    41161 tagagaccca tccgaataac tttgttcagt ggcgttttac tgctaaggag catccagaag
    41221 ctttttcggg tatccgatac tatcactcca cttttgatgc tataaacaac cagacaatga
    41281 agacattttt gcgaacgtcg atggcacagg atgcggtatt tcttcaagta tatggccaaa
    41341 gtgaatgcgg accaatgatt cttaaggctc atacactaga gagtttgaaa acaagtgatg
    41401 caagagatat gggcgttgga ctggaagaca tgacaagcgc tcggattaca gattcagttg
    41461 ggaatgtcct acctgaaaac acagatggtc atatacaatt actgtctaaa ggacgggctt
    41521 tgacttatta caaggaagat gcacggttcc aggaaaatgt ttatggcgat tggtgggata
    41581 gcggagatta cggttttatg gataagcatg gccacctatt tttgaaagat cggcaagtgg
    41641 atttgatcga aacgatcaat agtacattag ctattgaaga ctttttatta gattcgttag
    41701 attttttggc agaagtagtg attgtaaggg ataaaaatca ttctccacaa ccgattattg
    41761 ctttagctcc tgataaagaa atggattgga atcgatggtg ggagcaagtc catgaacttc
    41821 ctcgcttgaa cgtccctatc atccgtgatt ttgatgcaat cccgcgaaca gctacaatga
    41881 aagtgcaacg gcttcaaatc gagaaagaat taaaatcaca aaagtcctaa ctatttaagg
    41941 aaagaaagat ccattttggt ctttcttttc tttttgtttt tttaaattgg atgagataaa
    42001 aatatgccaa ttggctgttt ttcttttttg aaaatttcgt tatactctta ttcatggagg
    42061 aaaatgttat gaaaagaatc ttgacaattg gcggttctga tccatttgct ggtggaggaa
    42121 tccaaacaga tttaaaaact tttgagaatt ttggtctatt cggactaagc gccttgactt
    42181 ctataggggc actagatatt aatgagcgat ttatgctgga atctatatct ttggaattgc
    42241 ttaaacgaca gttagtttcg attgataaaa tgacttcatt agatggtatc aaaattgggt
    42301 tattgaattc aagtgaagca atcatggttg ttcgagactt cataaaaaca aaacaaaata
    42361 tcccaatcat tttagaccct gttttggcat tcaaagaaac tgaagctgtg atcaatcaat
    42421 tgtatatttc gcagctcatc gaagaacttt ttccacttgt tacgatgatt acgcctaatt
    42481 tggcagaagc tgctttactt tctggtcaag ctcttccctc ttcgttaaca gaaatgatcc
    42541 atctcgctaa agaactcact gcttcaggaa caaaaaacat tgtgatcaaa ggcggcacaa
    42601 ggatggctgg agatgaagct gtggatcttc tctattcaat tgatcattat gaggtatttt
    42661 ctcataaaaa aatcgacact acaacagtaa acggggccgg ttgtgccttt tcttctgcaa
    42721 taacagcgaa tcttgtgtta ggggcagatt ttaccaaagc agtagcagaa agtaaaaaat
    42781 ttgttgccca ttgtatttct aatggcgtgc tgatgaacga tcataccgga agtgtctggt
    42841 ctggcggtat tcaaaaagga ggaatgagaa atgaaaacta agcaattaac gatctatgct
    42901 atgctgattg cgttgacagt tgccctttca ttaacaattt tgatccctgt accggctacg
    42961 aatggatttg tgacattatg cgaggccggg atctataccg ctgcttttct ttatggtcca
    43021 acgggtggat tagttgttgg tgcttccagt ggtctattga tcgatttgat ttcaggatat
    43081 ccgcagtggg cagttttttc attagtgatc cacgggttac aaggatatgt tgctggttct
    43141 ttcagcaaat cttctactcg tatgtgggga ttagggcttt tattaggaag cttagttatg
    43201 atcatcggct atggtatcgc cggttggatt ttatatgatt ggccggctgg agtggcttct
    43261 ataccaggaa acatcttcca aaatctgatt ggaatgggac ttgcctttcc tataactgca
    43321 agtcttcaac gtattcaagc acggaagcaa tattaattga aaaagaaggt tttagaaaat
    43381 gaagttgtca gaaaaagaat taaaagacca attgactgaa atagttaatg acatattggc
    43441 tgaggcacac ttgaaaaaag gtgatctttt tgttttagga tgcagcacaa gcgaagtcgt
    43501 tggaggacat attggaaaaa attccagtgc ggaagttggt caatggatca tccgtacgtt
    43561 aaaagagttg ttggatccta aagaaattgc gctagctgtc caaggttgtg aacatttgaa
    43621 ccgagcatta gtagtagaac gaacagtggc agaagcaaaa aatttcgaaa ttgtctctgt
    43681 tgttcctgca ctacatgctg gaggtgcttg ttctgttgct gcttttgacc agttcaatga
    43741 tcctgtcgaa gtggaacacg tagttggaca agccggtatc gatatcggtg acacatcaat
    43801 cggtatgcat atcaagcatg tccaagtacc tgtcagacct cgtttgaaaa cccttggaca
    43861 agctcacgtg acagcccttc gctctcgtcc taaatatatc ggtggtccta gagcgaatta
    43921 ttgaaatgag taattgacag agcaatctga aaaacttcct cgcaaagtcc aactaatgaa
    43981 ctttaacgag gaagttttac ttgtttgttt tatataaccg agcttcataa ggtgctagta
    44041 tgcctttagc ttcaaattct ccgctagagg aatttgacaa gagtaatgtc caggactgat
    44101 ctgcttcttt tggaagttca tagtcagcaa gtgattctgt taagtttacc ataataagat
    44161 attgttcatc ttctaaatat ctttcataga cataaagttg tggatgttca tgaagatatt
    44221 ctttataaga accgtatatc agacctttat tggtttgtct aatgcggatc atgttcttat
    44281 agaattgcag gaccgactct ggttcatatt cttgttgttc tacattgatc gttttggtat
    44341 ttggattagt gatcagccaa ggttcatgtg tagaaaaacc agcatattgt tccgaagtcc
    44401 attgcatggg tgtcctgctg ttgtctctcg ttgtttcacg aatgatatcc aatgcttctt
    44461 ctcttgtctt accttccgca agcagccttt tataaagtct tttcgaatct actgcatcaa
    44521 cttgatcgat cgattcaaag ggcatattcg tcattccgat ttcctgtcct tgataaataa
    44581 atggcgtacc ttgcaaaaag aaatacatca tagcgattgc ttttgcagac atagcccaaa
    44641 aatcgggacg agtatctcca aacactgaaa cggcacgagg aacatcatgg ttttccatat
    44701 ataaagcgtt ccatccgata ccatccaatg aagtttgcca agcgcttaat gcatgtttca
    44761 atttaaggac atctaattgc ccttcttttt cttgctgcca gatatggata tgatcaaatt
    44821 cgaatatcat atcgaaataa ccgtctttcc ctacccattg tggtccttct tctgcagtga
    44881 caccacttgc ttctccgaca gtcatgatat cgtactcttc aaaaacatgt ttcaaatcgg
    44941 tcaaatgttc ttcgattcct gagacatttt gaaagggtga gaaagggttc tctgtggctt
    45001 ttactgaata ttcatctttt tttacatgag aaatcgcatc taaacgaaat ccatctatcc
    45061 ctttatccaa ccaccatcgg atcatattga atagttcttc tttcaatttc tcgctttccc
    45121 aatttaagtc tggttgttct tttgcaaaaa catgcagata atactgtcca gtttcatgag
    45181 aatactccca agcagaaccg ccgaaaatcg atgtccaatt attcggttct ttgccatcta
    45241 ccccgtcgac ccaaatgtaa taatcgcgat acgggctttc tttagatttt ttcgattctt
    45301 caaaccattg atgctgatcc gaactatggt tgatgacaag atccataatc actttgatcc
    45361 ctaattcatg tgcttctttt agcaataaat caaaatcttc catcgttccg aatctctctt
    45421 ctatatcttg atagtcactt acgtcataac cattatcgac aaatggagat tgatagatcg
    45481 gattgatcca gataaagccg ataccaagat ctttcaaata attaagtttt tcacggatcc
    45541 cattgatgtc tccttgacca tcgccattgc tatctttgaa actagcagga tagatttgat
    45601 agcctactgc ttctttccac catttttgtt cttgtatcat atcgtttcac tcctttaacg
    45661 gtccctcttg attataaccg attctatttt agaagtatcc ttgttaccgg taactagaaa
    45721 cgaaaagctc aacgaatctg ctaagtgcgt tcgttgagct ttattttatc atagatagcg
    45781 ggcaatcagt tgaatcattt gcttgggaaa ttcattcaag tcagtaatat ctacaaaacg
    45841 actttcaccg tagatcgact tgatttcttc tttgtcttgg ccaatagctg cggagacaaa
    45901 taggattcct tgacgttcat attctttcaa aacagactgg atatcttctt ttgctttttt
    45961 gcctgtataa tccggcaatg cttttggctg accgtcacta atattaagca acagtttggt
    46021 tgttgcggat tgtctgttta atttttctgc tgctaaacga agtgcagcac catcacgatt
    46081 gttttgcctt ggtttcatag tgactaattt ttcatttaac cattgaaatc cgtcttcaaa
    46141 ttctttataa gaaaacaaag aagtcttttc tcttgacgaa cggtctgctg tatctccgta
    46201 aatcaacaat gggatatcga ctttcttaga aaattcggca atggcaatcg ctgctttttt
    46261 tgccgcttcg atccgatcat cccgtatcat tgacccagat tcatcgatcc gtacagctac
    46321 cgctaaagaa ggttgctcat gtggcgggtt ctttttgtca aaatttctca aatcaccata
    46381 tgctactttg cttgcattga accgctgacc ttcgtatttt cctttttggt acgtctctgt
    46441 ttgttcatta tccagtaact ctaaaatttg ccggctaagt gattctacta ttggcattac
    46501 ttgcatgtgt agctcacgtg cttccggcgt ttttccttgg aaagctggtc ggtgaacgat
    46561 gagaccttct ttttcatgaa tcgtttcctt catgatttgt cgcgcttctt tattcagttg
    46621 cttcttgatt tccttatcat gtgcttccat gtcagaagca gacatttctt gagaatcatc
    46681 tcctgtttgt tgttcttttt gattttttcc ttcgatatcg ctactgcgtt ctttttgcat
    46741 ttgaggagga atctcgtttt tttctattgt ttcttcttgt ttctcttctc cctcatttgg
    46801 ttcttcagca gacaacgatt tcttttcatc taagcgcaga atttcttcag cattttcagt
    46861 gatgtttcgt tcatttgctt cttcggcgct aatggttctt tcttttgtat gctcagattg
    46921 aggatgaccc catttttctt ttctcaatat gtcctgaagt tccagtagca atccactgtc
    46981 agacaaagct tgttcaagaa ttgccaattc atctgaatca gtggataatt tatacaaaac
    47041 ttttggataa atcgactgga gtaaatcttg tccttgctta taggtatttg cccaataaaa
    47101 ataagaacgc attcctgcta cacctttgat ggcgttagct tttgcagttt catccaacaa
    47161 acggataatc tctgccatct ttaagagaag ttcttgtttt tggatcatag ttttcgccat
    47221 cgcacgttct gccatgactt ctaaggcagg aagatccatt ttttccgcat gttgcatccg
    47281 atctcttaaa gattcattta atgggcgatt tccttggtaa tttcgatttg tagtgatcac
    47341 aatgacgcaa tctggatggc gacgaatgat tcctgtcggt aaattcaaca tcccatttgg
    47401 ttcgagcgct gagttaagtg ccacaagaac agaggcatct cggatgacag tcggctcttg
    47461 aatctctaat aaatagcctt tttcaatcgc tcgaacgatt tcagaagggt aatagctgta
    47521 atggattgca tctggataat cagcttcgat cctttgaact agctctgcta atttttcttt
    47581 agcagaaaaa ggatcaatgc cgtaatggtt ctggatcagt gtcagtacag cttcaagtgt
    47641 atctgtttgg taaatagctt ctagtaattc ttggtcttgt tccttattag aatctactac
    47701 aggtaataag gctccgaaaa catctgattt gtccatatct gcaaaacagg tcacttttgt
    47761 atagggaagt tgcagatcag cagatagtgc tttggctaat tgtgtcttcc ccgacccggc
    47821 atctccttcc aaaagtacgt tactgatttt catttcagga tcttgccaat tccctttgat
    47881 ttcggaggcg atccgccatt cttctgcact tgtccgatgg cttttcggtt tcttccagat
    47941 catttgcttt tctagccgtg aaaaggttcg gttttctgtt agtgagaaat tctccatgct
    48001 tctcttcccc ttttgatcat ctctgtctat taatatccta gctcatctaa aagacgtttt
    48061 aaaatacgga atcgttcccc aatcagttct ccgtcttttt gtagtgtatc gtaatacaaa
    48121 atgatgtctt catcgatctt tggatttttg gtatctactt ctaccgtcat tcggttccct
    48181 tgtaatccaa tgatgtctac gactggattt tctggatcat aaaatttttc ataacgataa
    48241 gcatcttgga agtataaact tccttgcgca gctaagatcg ctaaatctaa aatacgtgtg
    48301 attggtaatt cttccgactg tcgtgaccat ttttctccag tatgtcgcca taccttgcct
    48361 gagatatcga cttttccacg atcattccac tgagcaaggc ccagtgaaag cccttttgca
    48421 tccatattta atgcatttct tccatcaact ttatcatagt cttctacaac gattactggt
    48481 ttatgtttta aatttgttgg tatttccatt attttaactc ctcatccttt tatttttagt
    48541 aaataagtaa attagtagtt tactcattta ctaaactata tgcgacatta tagaggattg
    48601 aagtgaggat tgcaaagaaa aagagcttca ttgatagaaa taaaaacacc atctcgttct
    48661 tttacacgct cattagtaac atgtagaaag tccaagatga tgttggaat
//
LOCUS       DAYYFU010000016        44939 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000016 DAYYFU010000000
VERSION     DAYYFU010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.016"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..1189)
                     /locus_tag="ACHA34_001080"
     CDS             complement(38..1189)
                     /locus_tag="ACHA34_001080"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFM8791738.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="ACHA34_001081"
     CDS             complement(1202..1639)
                     /locus_tag="ACHA34_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8791739.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="ACHA34_001082"
     CDS             complement(1654..3420)
                     /locus_tag="ACHA34_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFM8791740.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="ACHA34_001083"
     CDS             3684..4361
                     /locus_tag="ACHA34_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8791741.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="ACHA34_001084"
     CDS             4364..5491
                     /locus_tag="ACHA34_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8791742.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="ACHA34_001085"
     CDS             complement(5691..6131)
                     /locus_tag="ACHA34_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8791743.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="ACHA34_001086"
     CDS             complement(6142..8175)
                     /locus_tag="ACHA34_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFM8791744.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="ACHA34_001087"
     CDS             complement(8177..9880)
                     /locus_tag="ACHA34_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8791745.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACHA34_001088"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACHA34_001088"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFM8791746.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="ACHA34_001089"
     CDS             complement(10963..11988)
                     /locus_tag="ACHA34_001089"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8791747.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="ACHA34_001090"
     CDS             complement(12197..13066)
                     /locus_tag="ACHA34_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8791748.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="ACHA34_001091"
     CDS             complement(13525..15417)
                     /locus_tag="ACHA34_001091"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8791749.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISGQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="ACHA34_001092"
     CDS             complement(15414..17141)
                     /locus_tag="ACHA34_001092"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8791750.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="ACHA34_001093"
     CDS             complement(17157..17807)
                     /locus_tag="ACHA34_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFM8791751.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="ACHA34_001094"
     CDS             18011..18307
                     /locus_tag="ACHA34_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8791752.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="ACHA34_001095"
     CDS             18479..19099
                     /locus_tag="ACHA34_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8791753.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="ACHA34_001096"
     CDS             complement(19191..20357)
                     /locus_tag="ACHA34_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8791754.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="ACHA34_001097"
     CDS             complement(20369..20722)
                     /locus_tag="ACHA34_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8791755.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="ACHA34_001098"
     CDS             20850..21872
                     /locus_tag="ACHA34_001098"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFM8791756.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="ACHA34_001099"
     CDS             21891..22052
                     /locus_tag="ACHA34_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791757.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="ACHA34_001100"
     CDS             complement(22311..22745)
                     /locus_tag="ACHA34_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791758.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="ACHA34_001101"
     CDS             complement(22855..24714)
                     /locus_tag="ACHA34_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8791759.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            24715..24855
                     /locus_tag="ACHA34_001102"
     CDS             24715..24855
                     /locus_tag="ACHA34_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFM8791760.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(24832..25992)
                     /locus_tag="ACHA34_001103"
     CDS             complement(24832..25992)
                     /locus_tag="ACHA34_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8791761.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ACHA34_001104"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ACHA34_001104"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFM8791762.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="ACHA34_001105"
     CDS             complement(27739..28437)
                     /locus_tag="ACHA34_001105"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8791763.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="ACHA34_001106"
     CDS             complement(28394..30055)
                     /locus_tag="ACHA34_001106"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFM8791764.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="ACHA34_001107"
     CDS             30273..31673
                     /locus_tag="ACHA34_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFM8791765.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="ACHA34_001108"
     CDS             complement(31925..33004)
                     /locus_tag="ACHA34_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8791766.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="ACHA34_001109"
     CDS             complement(33090..34049)
                     /locus_tag="ACHA34_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM8791767.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="ACHA34_001110"
     CDS             complement(34061..35572)
                     /locus_tag="ACHA34_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFM8791768.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="ACHA34_001111"
     CDS             complement(35594..36157)
                     /locus_tag="ACHA34_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8791769.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="ACHA34_001112"
     CDS             complement(36163..37026)
                     /locus_tag="ACHA34_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8791770.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="ACHA34_001113"
     CDS             complement(37040..37936)
                     /locus_tag="ACHA34_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8791771.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="ACHA34_001114"
     CDS             complement(38111..39400)
                     /locus_tag="ACHA34_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8791772.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="ACHA34_001115"
     CDS             complement(39372..40889)
                     /locus_tag="ACHA34_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFM8791773.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="ACHA34_001116"
     CDS             41087..41977
                     /locus_tag="ACHA34_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFM8791774.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="ACHA34_001117"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="ACHA34_001117"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFM8791775.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="ACHA34_001118"
     CDS             complement(42886..44358)
                     /locus_tag="ACHA34_001118"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFM8791776.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT        12988 a         9388 c         7783 g        14780 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg cccgctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggatcaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt cttattggta
    44521 tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca cgaagaaatt
    44581 agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac aatcgtagcc
    44641 gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata atcaatggca
    44701 aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca aagaaacgga
    44761 tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga tataccttgc
    44821 gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc
    44881 agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc cttgttttt
//
LOCUS       DAYYFU010000017        43407 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000017 DAYYFU010000000
VERSION     DAYYFU010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.017"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..908
                     /locus_tag="ACHA34_001119"
     CDS             <1..908
                     /locus_tag="ACHA34_001119"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8791777.1"
                     /translation="VIFQLWIGGIFLIDVILLAKGTISITNFVIVFMYANMVPTLWTT
                     LISIIWKWQDYSVSLNRYYEYYSNNNKVAGTKKINKINSITGQSLNFSYNKRKVLSNI
                     SFSTEMNKLFMISGENGTGKTTLMKLFTKQLPVSNNQLFVNGIDINMIDNESLRRLIS
                     IATQIPYIINSSVKENILMGTKNYDKKKFSKLIELLGISKFMTKLGITLDDNIYDEKI
                     LLSGGEKQKISLARALIKSASVIILDEPFRNLDNETRDNLRDYILNTKKEVCYIIITH
                     DHILDNFLDSNDEILYLNKEYDNNE"
     gene            901..1929
                     /locus_tag="ACHA34_001120"
     CDS             901..1929
                     /locus_tag="ACHA34_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFM8791778.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            1985..2203
                     /locus_tag="ACHA34_001121"
     CDS             1985..2203
                     /locus_tag="ACHA34_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791779.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            2241..3089
                     /locus_tag="ACHA34_001122"
     CDS             2241..3089
                     /locus_tag="ACHA34_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791780.1"
                     /translation="MIKLRRNFKKIMKYNNLKPKLLNENIEYIREENYGYLTIVPKNG
                     TESKDNVFNPTVRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHY
                     NIIDIGDYSMEEKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNS
                     KELEVVLRYNLFSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTT
                     ILTTVLENINEVSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLSKEYREHDVL
                     RYAYIY"
     gene            3153..3488
                     /locus_tag="ACHA34_001123"
     CDS             3153..3488
                     /locus_tag="ACHA34_001123"
                     /inference="COORDINATES: protein motif:HMM:NF025015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM8791781.1"
                     /translation="MILLILSLVLLVVSNLMKNNPPTKINSFYGFRTKKSMKNEKSWH
                     LAQKLLGDFSKKKVIPVFLMGIVFFSIEIYSLAILKNENFFVSVLMGESLILILILCL
                     IVHEVNKQL"
     gene            3912..4175
                     /locus_tag="ACHA34_001124"
     CDS             3912..4175
                     /locus_tag="ACHA34_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791782.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
     gene            4509..4880
                     /locus_tag="ACHA34_001125"
     CDS             4509..4880
                     /locus_tag="ACHA34_001125"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM8791783.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
     gene            5183..5482
                     /locus_tag="ACHA34_001126"
     CDS             5183..5482
                     /locus_tag="ACHA34_001126"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791784.1"
                     /translation="MESQETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHN
                     YELIPEHWQLLGSSKQQGKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            5483..6937
                     /locus_tag="ACHA34_001127"
     CDS             5483..6937
                     /locus_tag="ACHA34_001127"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791785.1"
                     /translation="MFDESLHLFPEYPDTVGSQAIPEKKQAVCQILLKEEVQLKQELT
                     AVILMDRICKEVLAGSDGTLAYGVVPLFDEVVRLLGNTQQIGAICGEFLLLPKMLVTV
                     ELAELATGMNEHPYMSGYFYLTVDDKNRQALMQAVTRLLTEKKLNILVGTVSLLGEGW
                     DCPAINLLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVPLSEQPDLK
                     RMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSCERSRLKEQ
                     WSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKGNLLEYFQI
                     RRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLENAGYREGQ
                     LFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQSSLPKCQLV
                     YTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(7773..8444)
                     /locus_tag="ACHA34_001128"
     CDS             complement(7773..8444)
                     /locus_tag="ACHA34_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8791786.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            8761..10392
                     /locus_tag="ACHA34_001129"
     CDS             8761..10392
                     /locus_tag="ACHA34_001129"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8791787.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            10395..11474
                     /gene="uxuA"
                     /locus_tag="ACHA34_001130"
     CDS             10395..11474
                     /gene="uxuA"
                     /locus_tag="ACHA34_001130"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8791788.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            11548..12975
                     /gene="uxaC"
                     /locus_tag="ACHA34_001131"
     CDS             11548..12975
                     /gene="uxaC"
                     /locus_tag="ACHA34_001131"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8791789.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            13059..13706
                     /locus_tag="ACHA34_001132"
     CDS             13059..13706
                     /locus_tag="ACHA34_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM8791790.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            13835..15211
                     /locus_tag="ACHA34_001133"
     CDS             13835..15211
                     /locus_tag="ACHA34_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8791791.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            15222..16016
                     /locus_tag="ACHA34_001134"
     CDS             15222..16016
                     /locus_tag="ACHA34_001134"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8791792.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            16029..17795
                     /locus_tag="ACHA34_001135"
     CDS             16029..17795
                     /locus_tag="ACHA34_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFM8791793.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            17823..19631
                     /gene="uidA"
                     /locus_tag="ACHA34_001136"
     CDS             17823..19631
                     /gene="uidA"
                     /locus_tag="ACHA34_001136"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFM8791794.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            19803..20204
                     /locus_tag="ACHA34_001137"
     CDS             19803..20204
                     /locus_tag="ACHA34_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM8791795.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            20198..20443
                     /locus_tag="ACHA34_001138"
     CDS             20198..20443
                     /locus_tag="ACHA34_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8791796.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(20767..20988)
                     /locus_tag="ACHA34_001139"
     CDS             complement(20767..20988)
                     /locus_tag="ACHA34_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791797.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(21150..21222)
                     /locus_tag="ACHA34_001140"
     tRNA            complement(21150..21222)
                     /locus_tag="ACHA34_001140"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21187..21189),aa:Lys,seq:ctt)
     gene            21298..21528
                     /locus_tag="ACHA34_001141"
     CDS             21298..21528
                     /locus_tag="ACHA34_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8791798.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            21572..22741
                     /locus_tag="ACHA34_001142"
     CDS             21572..22741
                     /locus_tag="ACHA34_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8791799.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            23315..23884
                     /locus_tag="ACHA34_001143"
     CDS             23315..23884
                     /locus_tag="ACHA34_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFM8791800.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            23871..24731
                     /locus_tag="ACHA34_001144"
     CDS             23871..24731
                     /locus_tag="ACHA34_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8791801.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            24921..25625
                     /gene="yycF"
                     /locus_tag="ACHA34_001145"
     CDS             24921..25625
                     /gene="yycF"
                     /locus_tag="ACHA34_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFM8791802.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            25630..27465
                     /gene="walK"
                     /locus_tag="ACHA34_001146"
     CDS             25630..27465
                     /gene="walK"
                     /locus_tag="ACHA34_001146"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFM8791803.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            27462..28778
                     /gene="yycH"
                     /locus_tag="ACHA34_001147"
     CDS             27462..28778
                     /gene="yycH"
                     /locus_tag="ACHA34_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFM8791804.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            28779..29648
                     /locus_tag="ACHA34_001148"
     CDS             28779..29648
                     /locus_tag="ACHA34_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFM8791805.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            29703..30512
                     /locus_tag="ACHA34_001149"
     CDS             29703..30512
                     /locus_tag="ACHA34_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8791806.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(30615..31244)
                     /locus_tag="ACHA34_001150"
     CDS             complement(30615..31244)
                     /locus_tag="ACHA34_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8791807.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            31414..32706
                     /locus_tag="ACHA34_001151"
     CDS             31414..32706
                     /locus_tag="ACHA34_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8791808.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(32782..33444)
                     /locus_tag="ACHA34_001152"
     CDS             complement(32782..33444)
                     /locus_tag="ACHA34_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8791809.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            33669..33953
                     /gene="groES"
                     /locus_tag="ACHA34_001153"
     CDS             33669..33953
                     /gene="groES"
                     /locus_tag="ACHA34_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFM8791810.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            34004..35629
                     /gene="groL"
                     /locus_tag="ACHA34_001154"
     CDS             34004..35629
                     /gene="groL"
                     /locus_tag="ACHA34_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFM8791811.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(35718..36857)
                     /locus_tag="ACHA34_001155"
     CDS             complement(35718..36857)
                     /locus_tag="ACHA34_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8791812.1"
                     /translation="MWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWKKAQ
                     KILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHISDD
                     TIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPALAVK
                     IHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRYGEL
                     LALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIESVIA
                     DNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSHVSL
                     LSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(36968..37552)
                     /locus_tag="ACHA34_001156"
     CDS             complement(36968..37552)
                     /locus_tag="ACHA34_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFM8791813.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(37679..38110)
                     /locus_tag="ACHA34_001157"
     CDS             complement(37679..38110)
                     /locus_tag="ACHA34_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8791814.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(38128..38448)
                     /locus_tag="ACHA34_001158"
     CDS             complement(38128..38448)
                     /locus_tag="ACHA34_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8791815.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            38748..38888
                     /locus_tag="ACHA34_001159"
     CDS             38748..38888
                     /locus_tag="ACHA34_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791816.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            39186..39443
                     /locus_tag="ACHA34_001160"
     CDS             39186..39443
                     /locus_tag="ACHA34_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HFM8791817.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(39414..39722)
                     /locus_tag="ACHA34_001161"
     CDS             complement(39414..39722)
                     /locus_tag="ACHA34_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791818.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            39800..40546
                     /locus_tag="ACHA34_001162"
     CDS             39800..40546
                     /locus_tag="ACHA34_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFM8791819.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            40561..40764
                     /locus_tag="ACHA34_001163"
     CDS             40561..40764
                     /locus_tag="ACHA34_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8791820.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            40869..41168
                     /locus_tag="ACHA34_001164"
     CDS             40869..41168
                     /locus_tag="ACHA34_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791821.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            41168..41350
                     /locus_tag="ACHA34_001165"
     CDS             41168..41350
                     /locus_tag="ACHA34_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791822.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(41356..41598)
                     /locus_tag="ACHA34_001166"
     CDS             complement(41356..41598)
                     /locus_tag="ACHA34_001166"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791823.1"
                     /translation="MLNKNQRDVENIAIFLIKITSSISIIIPMTVTFSKEQFNVAATY
                     LSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            41885..42610
                     /locus_tag="ACHA34_001167"
     CDS             41885..42610
                     /locus_tag="ACHA34_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFM8791824.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            42784..43125
                     /locus_tag="ACHA34_001168"
     CDS             42784..43125
                     /locus_tag="ACHA34_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791825.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            43118..>43407
                     /locus_tag="ACHA34_001169"
     CDS             43118..>43407
                     /locus_tag="ACHA34_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM8791826.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT        15121 a         6488 c         8524 g        13274 t
ORIGIN      
        1 aggttatttt ccaattatgg attggaggaa tatttcttat tgatgttatt ttattagcta
       61 aaggaacaat aagtattact aattttgtaa tagtatttat gtacgctaac atggtcccta
      121 ctttatggac tactttaatt agtattattt ggaaatggca agactattca gtgtcattaa
      181 atagatatta cgaatattat tcaaataaca acaaagtggc tggcacaaaa aaaataaata
      241 aaataaattc tattactggt caatcattaa atttttcata taacaaaaga aaggtattaa
      301 gcaatataag tttttcaact gaaatgaata aactattcat gatatcagga gaaaatggaa
      361 cagggaaaac aacactgatg aaattattta ctaaacagtt gccggttagt aataatcaat
      421 tattcgtcaa cgggatagat attaatatga ttgataacga atcattaaga aggttaattt
      481 caatcgcaac acaaatacca tatataatta atagctccgt gaaggagaat attttaatgg
      541 ggactaaaaa ctatgataaa aaaaaattca gtaaattaat agaactattg ggaataagta
      601 aatttatgac gaaattaggt attactttgg acgacaatat ttatgatgaa aaaatattat
      661 tatctggagg agaaaaacaa aaaataagtt tagctagggc gcttattaaa tcagcttcag
      721 ttattatatt agatgaacca tttagaaatt tagataatga aactcgcgat aatttgaggg
      781 attatatact aaatacaaaa aaagaagttt gttatataat tataactcat gatcacattc
      841 tagataattt tttggattca aatgatgaaa tattatattt aaataaggag tatgataata
      901 atgaatagca aaaaacttca gatgggtgaa attgttagag gtcctagtca aatatctatt
      961 gatgttacat ataaatgtaa ttttagatgt aaacattgtt acaatgcgag tggtggagac
     1021 aattgtttta tggataatga gttgactgat gatgaactgt tagaacttac aaaagatata
     1081 tgtaatttaa agccatataa tatttgtttc tgtggaggag aaactttact gagaaaaaaa
     1141 atagttttat cttgtgccac tttattaaaa gaggcaggta ttcctaatat tgctattgtt
     1201 actaatggat cattagtaac tcaggatgtt gcaaaagaat tattcaaagc aggtattaat
     1261 cgatttcaag taagtttaga tggaaaaaat gctagtagcc atgaagcact tagattatat
     1321 ccaaacagtt ttgaaaaggc aattaatgct atcaaattat ttagagaatt gtccgaagaa
     1381 gtggatgttg ctttttgtcc tactagtttt aattacaatc aattacgaga agtatatcaa
     1441 ctagtaaata ctcttaaagt gaagtcttta agaattcaac catttatgct tagtggtagg
     1501 ggaatgcaaa atagggaaat agtgccaact gtttctcaat atagaaaact agtttctgat
     1561 attaactata ttaaaagaaa tgaagattat accaagatag attggggaga tccaattgat
     1621 catatcatcc gacacagaac tttattgaaa gatttttcga attttgtttc tattaggtca
     1681 gacggaggat taatggcatc accttacttg ccaattcaag ttggaaatat taaatgtcac
     1741 agtctttcta aatattggcg agcaggactt tataaagttt tttctttaga tttagttcaa
     1801 gatatagcta ctcaagtttc ttcaatacaa gatatgagta aagaattaaa aaaaggacat
     1861 attaaatggc ttaatgaaga tatagaaatt gatattctcg atgaatacaa acatgatatt
     1921 ttaatttagg tttatcattg gaaagtttag ataaacaaag tacttttaga aaggagggaa
     1981 aaaaatgatt tctgaacctc aaatgaataa tatgtatgaa agcccaacta taatgggagc
     2041 tggtgaagct gcgccaagag cttgttcagc aattatggtg tgtgtgttat tagcatctgc
     2101 aggagtaggt atccaagcag gagcggtgta tacttatgct gtagttgcta cagtagctgt
     2161 agctgcagcc gcagtttgga aaacagtagg attagttggt taggaattaa taggagacta
     2221 actgtctcct attgattcca atgattaagc taaggaggaa ttttaagaaa ataatgaaat
     2281 acaataattt aaaaccgaaa ttacttaatg agaacataga atatataaga gaagaaaatt
     2341 atggatattt aaccatagtt cccaaaaatg gaactgaatc gaaagataac gttttcaatc
     2401 caacagtaag atatatattg gctaactgta ctggaaaaaa cagtgtaaaa aaaatagcga
     2461 atggtttaaa aaagaagttt cctagtttag aatatgaaaa attctatcaa gatgttgtag
     2521 gaactattaa atttttcaaa cactataata ttattgatat aggagattat agtatggaag
     2581 aaaagaaaat atataaaatt gaacaaaatt tagaattaga attactaaat gaatcacatt
     2641 atgaagaaat ttataatttt atcacagatc atcctgaaag tatgtatttt catgaagata
     2701 tttatcatca aggaaatagt aaagaattag aagttgtcct tagatataat ttattttcgt
     2761 ttacgagtga attttacttt ttaagacata aagagagaat tgtaggaatg gtttctataa
     2821 ttgttaatcc atttcaaaac ttatcctaca gtatagtaaa ttacctcata tgtgagcctg
     2881 catatactac gactattttg acaacagtat tagaaaatat taacgaagtt tcaattttag
     2941 aagtaaagaa gctagtgatt catttgctaa attcaacaga agatgaatca ctaatcccct
     3001 atttaaaaga ttcacatttt tttaaagaag cggtactatc taaagaatat agagagcatg
     3061 atgttttgag atacgcttac atttattaaa taaaatttct gatagtattt taataattcg
     3121 gatcatatca taagatatag gagggaaaag aagtgatttt attgattttg tctctcgtat
     3181 tactagttgt atctaatcta atgaaaaata atcctcctac aaaaattaat tctttttatg
     3241 gctttagaac taaaaagtca atgaaaaatg aaaaaagttg gcatttagca caaaaattgt
     3301 tgggggattt ttcaaaaaaa aaagttatcc cagtattttt gatgggtatt gtttttttca
     3361 gtatagagat ttatagttta gctattttaa aaaatgaaaa ttttttcgtt agtgtattaa
     3421 tgggtgaaag tttaatccta atactgattt tgtgtttaat tgttcatgaa gtgaataaac
     3481 aattgtgaat taaaaaaata aaaacctagt ataatcactt taataaattg ttggtagaag
     3541 gtgatcaaaa tgggagtaaa cgaaatgatg aaagtcagat tgagttttta aatgatacct
     3601 tcctcagaac ttcaaaacga attcaaagat tcgttgacaa atcttgagca agatcattta
     3661 aaaaaatatt ttctcttcat ttgacgaaaa atgtttagaa atcttatata agtgaagata
     3721 aagtaattgg tgtaattaat tgtagagaat agcctttgag caattgaagc gatacaatta
     3781 tttagaaaat gaacttcctc cagcatacta catgattcct tacgggacga tgttaaagaa
     3841 gaaagcaaga aattttagaa gtattagtag ataaactaat gggcttttct caatttaagg
     3901 gagtagccca aatgtttatc aaatatggtg cagatacagt agcaaccatg tttacagaag
     3961 aatatcaagt agactattat gaagtaatag ataaatgtgt acattttgtt tatgaaaaag
     4021 tgtggaattt tatgaatttt gcgataagtg aagaaatctt acgagtacaa gaaaacagaa
     4081 caggaatatt taccagagac agaaagctta atttgattgc gcattatata cggaatcgct
     4141 ggtcatcaat accaaatttt aattataaaa aataattatg aacaatcttt tatacaatat
     4201 acacaataaa tcaagtagta tttatgaaaa aagacttcaa aaacagaatg attttctatc
     4261 agtcttagaa attatagagc taaaaatttt ggtcacgcgt agaaacgcgt tttttttgtt
     4321 tttttgatag taaagaacgt acaaaacaat gagaatttaa aagattgtac taccagtttt
     4381 tttacctttt catttgagtt tagagtaagt aaaatattac tataaaaaca catattcttg
     4441 aagaaatctt ttttttgctg tattcattta gattatgata gagaaaatac gtgagagggg
     4501 gacctgggat gtggctatct gatgaaaaaa ttcagtggcg tagctatcag aagcgtatat
     4561 tgactaactt tgctacttat ttaaataaca attcagtcca tctagtggct ccaccagggg
     4621 ctggcaagac ctttttagga attgagatga tccgacgttt ggattgtaaa accttaattt
     4681 tggttccttc cctaatattg aagaaacagt ggttaacaac aatcgaggaa tatttcttag
     4741 taaatccagc agatagttgc tatctttctg gggcgattga acatcctagt catattacag
     4801 tggaaacgta tcagaccatt tacaaccgac tgaaggagga gccaacttat ggtgatcagc
     4861 tggagctgtt agttttttga tgaagctcat catttaaaaa aaagctgggg tgaattgttg
     4921 attgacttga agaaaaaaca accgaatctg ttgacgattt ccttgacagc cacgcctccg
     4981 attgatagct ctcagacgga gtggaagcaa tatattgagt taaaatggcc tgattgacga
     5041 agaaattagc attcccgagt tggtaaagga ggaggttcta tgtcctcatc aggattttct
     5101 atatttgatt ccggctaatc aggaaactac tcagcattat tttacctttt tagagaaaca
     5161 aaatgagctg gtggaagcgt taatggaaag ccaagagacg gtggattatt tgctttcttt
     5221 agaatttgtt cagcatccgc ttgagcattc gaaggaaatt tatgaaaatt ttgagctgtt
     5281 cactagtgca ttaatttact tgaatcatca taactatgaa ttgatacccg aacattggca
     5341 gctattaggc agttctaagc agcaaggtaa aaaagcttca gttccatctt taggtaaaaa
     5401 agagctgatc actatttatc agcagatgtt tgaagatcaa ccggaattgc ctatttttta
     5461 tcaattgaaa aaagctggat gattgtttga tgagtcgctg catttgtttc cagagtatcc
     5521 agacactgtt ggtagtcagg cgattcctga gaaaaagcaa gcagtttgtc aaattttgtt
     5581 aaaagaagaa gttcagctga agcaagaact gacagcggtc attttgatgg atcgtatttg
     5641 caaggaggta ttagcaggca gtgatggtac cttagcttat ggcgtggttc ctttgtttga
     5701 tgaagttgtt cgtctattag gtaacactca gcaaatcggt gccatctgtg gagagttctt
     5761 actgttaccg aaaatgctag taactgttga gctggcagaa ctagcgacgg gtatgaatga
     5821 acatccttat atgtcgggtt atttttattt gacggtggat gacaaaaacc gccaagcatt
     5881 gatgcaagca gttactcgcc tattaaccga aaagaagttg aatattttgg taggaaccgt
     5941 tagtttgcta ggagaaggct gggattgtcc tgcaattaat ctgttgatct tggctaatca
     6001 agcttccact tttgtgcaaa cacaacaact acgagggcgg ggcttacgcc taggtgcaca
     6061 aaataaagta acgaacattt ggcatttagc gattggatta ccgggagttc ctctgtctga
     6121 acagcctgat ttgaaaagaa tgctacggcg tttgggcaat atttcaggtc tgacctttga
     6181 atcttcacca aaaattgaga gtggtggcga tcgctttgct ttaccactgg aatatacgac
     6241 caccacgatg gaaacttata ctcagtcgat gttctatttt tcttgtgaac gaagtcggtt
     6301 gaaagaacaa tggagtactg ctttagctga tggctggcac ctatctatgc caatcgtcgt
     6361 taggaaagtc gatcaggtaa agcaaaaaca agaaacttca ttgccgatag tagtaaaaaa
     6421 tatagagcct ctaagcttct ggtcagctat atttaaaggt aacttgctgg aatatttcca
     6481 aattcgtagg cgacaaaagc tatgggaaaa ggaatgtgcc cgcaaaaaaa gcttggccat
     6541 atgcgttcaa acgacgttgc agcgccttgg tgaagttagt gatagaaaag taatcgtagc
     6601 ggctgatcag caggaattta gatgctatct tgagaatgct ggctatcgag aaggacagct
     6661 ttttactgaa gctatgaggg agcttttagc accgctggag aatccgcggt atttaatcga
     6721 agggaaaaag atagttctga cggtaccaga acgctttggc cgaaataagc aaatcgctaa
     6781 cctttttcta aacgaattaa atcaatcgtc gctgcccaaa tgtcagttgg tgtatactag
     6841 aaatcctgaa ggacgccaaa agctagtgac ttatcgaatg aggcaagtct tccagacccg
     6901 cgatgaagta attattcaaa aaaacatttg gacgtaagtt gatgaaaata aattttgcta
     6961 gagtagaaca agtccgagaa ttaagttgct acttaactga taatgccaag gacaggagaa
     7021 tcttttcttg acgactgaaa gaaactattt gacttatata acggtgtgtg atgaatattt
     7081 gcaaggcatt ctcttaaaaa gtactcgtaa tagttttttt gaaaacaaaa cagtacttta
     7141 ttaataggac tgacagagcg atataagaac atctgcagac agaggtgaat gcttttctca
     7201 tgatcgatga aacaatggta cttcggatat tcttgctttt agtttgaacc gttgtgattt
     7261 tctttgattc ctaaaagtag ttttatagaa tgaaaatcaa gcatacggaa tgctcctttg
     7321 attgtgttgt ggtgattcaa tctaatggag cttctatatg tttttttatt tgcaaaaaga
     7381 accgatagaa tatttttatc agtcttagaa attatagagc caagaaattt caagcacaca
     7441 taaaaacgcg tgttttatgc attttatgag caaaaataac taaagaccat tacgtaaaac
     7501 ataggtaaat cagcattcaa tctcaaatta tgtgatcaaa tgttgatatt atttgattat
     7561 aagttttctt ttgctattat cagtctgcaa ataaacagat tatagagtat gaatgtagat
     7621 agttcaatct ttgtttacga cctcacatgt ttttttactc aagagaaagt aatataaagt
     7681 gactatattt gacagaactt tacataaaaa aagtagttag ttttctgtag taatcagaaa
     7741 tgtaaataaa ttaaaaaaaa gaaatttagc agttatatat ttttaaaata ttttgggtga
     7801 gtagtcaata caacattttg ttcatcaagc attaaatgaa gatgcaagga tgttaagaag
     7861 ttagcttctt cagggtcttg catggtcaag gctttaaata gttgatgatg ttgttccata
     7921 attttttccc attttaatcc ttccgttttt actcgtaacc aacgaaatcg ttccaagtgc
     7981 gtattattat cttccaacca ttgccatact tcttcccgtt ctgcaatttc aaaacaagct
     8041 ttatgaaata aattatcatt atgaaaaaaa ctacgagcat tcttttgact aacagctttt
     8101 tcttgttctg caattatagt ttctaaaact tgtttctttt gattattcat cttggcacaa
     8161 cactctagca taatttgtcg ttctaaatgt tctctgacaa aacgagcatt ttttgctaaa
     8221 tctaagtcga ttaatgagat ataatttcca ctttgaggaa ttgtatcaac aagtccttga
     8281 tgacggagtt gaatcaatgc ttcacgtatt ggagtacgac cgatagaaag cgtttcttcc
     8341 aatttttttt ctgatacttt ttttccaggt tctaagtcag cgtagataat ttgctgacga
     8401 atagattgat aagcttgatt ttggagacta gttttaattg tcatatttaa tccctctcaa
     8461 aatttcattt ttgaatttga tattttagtt ctatgctcgt tatataatat caaatttcaa
     8521 agtaacatac tagctattaa aaaaacaaaa aatactcaaa taagatcaaa gagaaattaa
     8581 gctttaattt aagttagttc taaaaaatat atattggtgt gcttttacta aaaaaagaca
     8641 caataattct gacatggtag aaaaaagaat atttttagtt ttttcttatt gacagcgatt
     8701 acattcgatt ttatactaca attgtaacgg aaactaacat atcagtatgg aggggtaaat
     8761 atggttttga ttaaagactg gcaagattct atcgaaggat tgaacaaaat gcaagttcaa
     8821 ctaccgtatt ttaatgtcga agcggtcaaa caagaaggtc agaaacatcc agtgtggctt
     8881 cattttggtg gaggaaatct ttatcgagga tttcatgcag aaattgctca atgcttagca
     8941 gatcaaaatg aattagaatc cggagtagtt gtgtgtgaga catttgatga agaagtagta
     9001 gaaaaagctt atcatgacta caacaatgat attttggaga ttgttatggg tgaagatggt
     9061 gaactcaaaa aaagactttt agcagtaaca gctgatagtt tatattgcaa tccaaagaat
     9121 ataacggact atcaaagagt ggttaaatat tttgaagatc ctacattaca gtttacaacg
     9181 tttactatta ctgaaaaagg atacagttta aaagattcta aaggacaatt tttttcggta
     9241 gtggaagcag atataaaatc tggacctacc aatcctcaac atacgatgag tattgtgaca
     9301 tccttgttgt atagcaggtt taatgctgga gagttaccga ttgcgatggt ttctactgat
     9361 aatttctcac agaatggtca acgttttcaa gatagcattt ttatgattgc agaagcttgg
     9421 caaaaaaaag gatacgtttc tgaaaaattt attgcttatt taaaggataa agaaaaagtg
     9481 acttttcctt ggagtatgat tgatagaatt acgccaaatc cttcggaaag tgtcaaaatg
     9541 gatttattga aggcgggttt cgaagactta gatataattc atactgcgaa aaataccaat
     9601 atcgctgctt ttaccaatac tgaaaaagtc cattatctag tcattgaaga tagttttcca
     9661 aatggtcgac ctaaactgga agcggctggt gtgattatga cagatagaga aacagttgat
     9721 aaagcagata tcatgaaggt tacgacatgt ttaaatccgc tccatacggc attagcaata
     9781 acaggttgtt tgttggggta taaatcaatt caggcagaaa tgcaagatga tgatttggtt
     9841 ggcttaatta aagggatagg ttatgtagaa ggcttaccag tagttgaaag tccaggcgtt
     9901 attgatccaa aacaatttat tgatgaagta atcaataaac gtttgcctaa tccctatatt
     9961 ccagatacac cgcaacgaat tgcaacagat acttctcaaa aagtagcgat tcgctatggt
    10021 gaaacaatta aaaagtatgt cgaaaaagac ggaaatgcag aaaatctaac atttattcct
    10081 atcgctattg ctgcttggtt acgttattta ttggcagtcg atgatcaagg aaaaaatttt
    10141 gtgccaagtc ctgatccttt attggtagag ttgcaggaaa aattctcagt attgaaatta
    10201 ggtgaacaag atcctgcaaa gattcattgt gcggtacagg aaatcttaga aaataaggaa
    10261 atatttggtt tagatttata tcaagttgag ataggaaaga aaattgaaaa aatcttttgc
    10321 gaaatgctag taggtcctca agcagttcgt aaaacaatca ataaatatat aacagaagtt
    10381 ggaggaaagt aactatggaa atgactttta gatggtatgg ggcaaatgaa gaaaaaatct
    10441 cactacaaga aattcgtcag atcccagcaa tgaaaggaat tgttggaaca ttaatggata
    10501 taccagccgg agaggtctgg ccgagagaac gaattaaagc tttaaaagat gaagtcgaag
    10561 ccaatggcct taccttgaaa gttattgaga gtgttaatgt tcatgatgat atcaaaattg
    10621 gtttgccaac acgagatcaa tacatcgaga attataaaca gacgattcgc aatttggcag
    10681 aatttgggat tgaggttatc tgttataatt ttatgccagt ctttgattgg gtaaaatcag
    10741 atttggatta tcatttagat gatgggtctt ctacgttagc ctatatttca aaaaatattc
    10801 ctactgatcc agaggaaatt attagaaaag tagaaaattc tactaataat tttgaattgc
    10861 cgggttggga acctcatcgg ttatcacaaa tcaagcaatt attagaagcc tataaagaag
    10921 ttgatgaagc gaaattgcga gagaactttg catattttct caaagcaatt atcccgacat
    10981 gtgaagaagt tgggattaaa atggcagttc atccagatga tccaccatat tctattttcg
    11041 gattacctcg tattgttaaa aatcgtgacg atttagaatg gttgtgtaat gtagtggatt
    11101 ctccttctaa tggtattacg ttatgtactg gaagtattgc agaacgacca gataatgatg
    11161 tctatgaaat tctatctgaa ttttgtcaac gggatcggat tccttttgca catgttcgta
    11221 atatcaaata tttaagtgat aatggactcg atttttatga agcgccgcat aaatcagaat
    11281 atggttctct agatatgtat aagatattaa aaacaatgta cgataatgga tttaatggct
    11341 atattcgtcc agatcatggt cggatgattt ggggagaaac cggtagacct ggttatgggt
    11401 tatatgaccg agctctaggt gcagtttatt taaatggtat ttgggaagcg cttgaaaagg
    11461 gggcgagtaa ataattatat aatggagtct atttagaaga aaaattttct cttctaaaaa
    11521 ctaagggaag gaaaggagtg cttaaatatg tttttagata agaacttttt attagagaat
    11581 gaaatagcta aaagactttt tcataaattt gcagaacacc aaccaatcat cgattatcat
    11641 tgtcatttag acccagcatg tttgctagaa aataagcaat acaagaattt gactcaggta
    11701 tggttaaatg ataaaggagc tggagatcat tataaatggc gattgatgcg agctaatggg
    11761 atagaagaag agttgatcag tggtgcaggt aatgattatg aaaaattttt agcttttgta
    11821 aaaacagtgg agaaggcgcc aggtaatcct atttttgaat ggtctcattt ggaattacaa
    11881 cgttactttg atattgattt gacaatttct gagaagaatg caccagaaat ttggcgacgt
    11941 gccaacgaaa aaatccaaac ggattcctat cgcccaaaag aattgctgaa gatgatggat
    12001 gtgaaagcca tttgtacaac ggatgatcct acagatgact tgcgatatca tactgagctt
    12061 tctaaacagg ataattcttt caaggtatta cctactttcc gtccagataa ggtctggagt
    12121 atcaaatcat caggattttc gaactatatt ttaaaactta gtcaagcagc gggtatacca
    12181 atcaaaaact ttaatgggtt gaagcaggca ttgagccaac gagtggatta ttttcatcaa
    12241 attggctgtc gattagcaga tcacggtttg aatacttatt tttatgaaat agcagatgaa
    12301 aaaatactcg acaatctgtt aataaaagct ttagcaggaa aaacggattt ccatttatca
    12361 gaaattgcac aatttgttac aggtattcaa ttacatttga tgtctgagta tacaagatat
    12421 ggttggacga tgcaaatgca tttaaatgct ttaagaaata attcaagtat attaaaggaa
    12481 caaattggag tagatgtcgg tggtgattcg atgggggatc aagctagttt gacacaaaat
    12541 gttgtagcat tgttcgacac tgctgaaaag agtgggtgct taccaaaaac aattttatat
    12601 tcattaaatc caaccgattg gcttccacta gcaacagcaa tgcaaagctt ccaagggggt
    12661 atgattcaaa aaatccaatt gggatgtgct tggtggttca atgatacagc tgaaggaatg
    12721 cgtcaacagt tgactacgat ggcgcaacaa agtttgttgt cgaattttac aggtatgttg
    12781 actgattctc gaagttttct aagctatcct cgccatgagt atttccgtcg agtattatgt
    12841 caattactaa gcgagtgggt taaagcaggt cgaattccag atgatgaaat gtatcttagt
    12901 cagatagtgg agaatatttg ttatcgtaat gcgtataatt attttaattt ttttgaaatc
    12961 gaaagtagaa aataatacaa aaaataaaag cttaaattta tttattgtaa gttagtacaa
    13021 ctagctagag tatgtatcca taaaagaagg agatagatat gaaaaaagta gaaattttaa
    13081 gtaagttaga agcggctgga gtcattgcgg ttgtacgtgg aaaaaataaa gatgaggcca
    13141 ttaaagcaat aaatgcaatt actgcgggtg gaattaaagg gattgaacta acttttacag
    13201 ttccgaatgc tgaagaaata atcaaagaaa ttgctaaaaa atatgctgat caaaaagatg
    13261 taataattgg agccggtaca gttttagatg cgtttactgc tcgtattgca attatggctg
    13321 gagcagaata tatcgtaagt ccatcttttg atcgacaaac agcagagatt tgtaatttat
    13381 atcaaattcc ttatttacca ggttgtatga caattactga gataaaggag gcacttaccg
    13441 ctggagtaga aatcattaaa ctatttccag gttctgcttt tggtccatct gttgtaaaaa
    13501 cgtatcaagc tccattacca tatgtaaata taatgcctac tggcggagta agtctagata
    13561 acttagaaga gtggtttgaa gcaggcgttg ttgcagttgg tgtaggtggg aatttgttgg
    13621 ctcctgttga aaaaggtgat tttgaagaag taactcgaat agctagtaaa tatatagata
    13681 agttttatga gataaagaat tattaggaga aaaaataaat aatactataa aatgtaaaaa
    13741 ataaatacta actctttata cttcagagta tagtacgtaa gttggcagtt caacagaatg
    13801 aaaacgctta acaataggaa ggagcgcaaa ttatatggaa agaaaatttg gtctaagaga
    13861 caaaatagga tatatgtttg gagattttgg gaatgatttc actttcattt ttgctagttc
    13921 ttttttaatg attttttata caaaagtatt ggggataagc ggagctaccg taggaacatt
    13981 atttttagta gcaagattta ttgatgcagt tacagacata acaatggggc gtattgtgga
    14041 tggatcaaaa ccatctaaaa ctggcaaatt taaaccgtgg attttacgga tgagtggtcc
    14101 agtcgcttta gcaagttttt taatgtatca aacatcaatg gcatcagctt ctatgagtat
    14161 taaagttata tatatgtatg taacatatat actatgggga agtatttttt atacatctat
    14221 taatattcct tacggttcaa tggcatcagc tatcacttct gttgcagaag aaaggacagc
    14281 gttatcaaca tttcgtagta taggagctac cttagcaggt atgttcattg gcgtgttaac
    14341 accaattttt atttatacaa gagatagttc tggagctcaa attgtacgtg gtggatcaac
    14401 ttttacaatt gtggcaggag tattttcagt gtgtgcagtt atttgttacg taatatgtta
    14461 taaattaact gtagaacggg ttaaaagtga tgaatcgcag ttaaaagttt cattgttgaa
    14521 atctttaaaa ggattagcta aaaatagagc gttgctttct ataatagctg cagctatttt
    14581 tttgttgctt gctcagcttt taacaatgag tatgaacaat tatgtatttc cagattatta
    14641 taataatgca cagggaatag ttttaatgaa ctttatcaat cctattttga tgttggtact
    14701 tgtttcacct gtaagtttaa ttttcagtaa aaaatacggt aaaaaagaat tggcgtcagt
    14761 agggatgttc ttctctgcta ttgtatattt acttttattt ttgataaagc ctagtaatat
    14821 gtatgtattc ttagttctta cttctgttgg atatatgggg ttaggtattt ttaatacagt
    14881 catttgggcg aatattactg atgtaatcga tgatcaagaa gtgaaatctg gccaaagaga
    14941 agatggaact atttatgcag tatattcttt tgcccgaaaa attggacaag ctttagctgg
    15001 cggtgtcgga ggttggactc tttctataat agggtatgat tcgttagcaa aagtacaaag
    15061 tggagaagtt ttacaaaagc tgtataactc ttcaacgttg attccagcag tttgtttctt
    15121 tgttgtagga ttgattttat ttttcgtata tccattatct aaagaaaaag tattagaaaa
    15181 ttataatttg ctaaatcaaa aacataacta ggagaaaaaa catgaacagt tggatatttt
    15241 gtattgattc agatggttgt gtcatggata caatgactta taaacacaat ctctttttcg
    15301 ggccaattgc tgcagagatt tttgatgttc atcataggga aaaattccta caggattgga
    15361 ataatattaa tttatattca gagactcgtg gaataaacag atttttaggt ttagttaaaa
    15421 cattagaaaa aaatgatgta ccatcgatta atcgattaaa gcactgggta tctgtaacaa
    15481 aagaattgtc taatccatct ttgaagaggg aaatagaata ctctcagtca gaggatttat
    15541 taaaagcttt aaaatggtcg gaagaagtca atcgacagat tagtcaagct aaaggattag
    15601 ataaaccctt tttaagtgct agagatacta taaaagctct gaaaaaatat gggaagataa
    15661 ttattgtaag ctcagctaat aaggaagcta ttcaagaaga atgggaaaga catgaattat
    15721 tatccttagt tgatgaactg tgttgtcaag ataaaggaaa aaaagaagac attattcgaa
    15781 gtgtaattga aaatggttgt gatttggata agatattaat gattggtgat tctcctggtg
    15841 atctagaagc tgcaaataag aataaagttt tcttttatcc tattttggtt aataaagaaa
    15901 aagaatcatg ggaaaactta agaactgata ttttatttaa atgtttatcg ggagagtata
    15961 tagatattga acaaaaatat atagatactt tttggaaaaa tcttactaat aagtagagga
    16021 gaaagaaaat gactataaaa gtagacttac gaaaaaaacc atatcattta gatgaagatg
    16081 ctatttcttg gattgaagag acgattgatg ctatgacttt agaagaaaaa ataggtcaat
    16141 tatttgttaa tatgggatct agtcgtgata aaaattactt aagtagtatg gtcaatcagt
    16201 accatattgg agctgtacga tataatccag gaactgctga tgaggtatat gatcaaaact
    16261 atatcctaca aacagaaagc aaaattcctt tattaattgc tgctaataca gaagcaggtg
    16321 gtaacggagc ttgtacagat ggtaccgaag ttggtgtaga agtaaaaatt ggtgcaacaa
    16381 atgatgcgaa atatgcttat gaattaggga gagtttctgg aattgaagca tcggctattg
    16441 gatgtaactg gagttttgct cctattgtag atattaatag aaattggaga aatcctatca
    16501 tttctagtcg atcttttgga caaagccctg ataaagtttt agaacttagt ttagcttata
    16561 tgaaaggtat tcaagagagt ggaattttgc ctgcagctaa acatttccct ggtgacggta
    16621 ttgacgaacg agatcaacat ctatctttta gtattaatag tttaagtaca gaagaatggg
    16681 ataattcttt tggaaaagta tactcaggct taatagaagc tggattacct tcaattatgg
    16741 caggtcatat ccatatgcca gcttattcaa aacattttaa tcttgatctg aaagatgaag
    16801 atttacttcc tgcaacttta tcaaaagaat taatcactga cttacttaga ggaaaactcg
    16861 ggtttaatgg attagtagtt actgatgcta gtcatatgtt aggaatgact ggggcaatga
    16921 aacggtcaga attattgccc acttctattg cggctggatg tgatctattc ctatttttta
    16981 atgatccaga tgaagacttt aattatatga tggaaggtta ttacaaagga attatttcag
    17041 aagagcgttt acgtgatgca ttaagaagaa ttttaggctt aaaggctaaa ctaggtttgc
    17101 ataaaaaaga aaagaataaa attttgttac cgaaacaaga agctatggaa aaaattggtt
    17161 taattgaaaa taaatcaatt tttaaagagg tagctgatca agcaattaca ctagttaaag
    17221 ataaacaaga tatttggcct attgtaccag aaaaatataa aaggatttta ctggtagatg
    17281 tacatggggt aaaaggtggg tttggtgaag tagttgctgg aaatagacct caggccattg
    17341 atttattgaa acagattttg tcagcaaaag gatttgaagt tgaaaaatac atttcacctg
    17401 aagaagaatt gaaaggatta tcgcaagaag aaaaaattaa aaatatagct ggaatatatg
    17461 ctgctaagag accaatcaaa gatttaactg aaaaatatga cttgattatt aatatagcta
    17521 atgttaaccc aggtacagtt caaagaattg tttggccagc aagtaaaggt actccggata
    17581 ttccatttta tattcatgaa attccaacaa tttttgtatc tgtacagtgt ccgtttcatt
    17641 tagctgatgt gcctcaagta aaaacttata tcaatactta tgatggaaaa caagaaactt
    17701 tagaaatatt agtggacaaa ctaatgggcc tttctgaatt taagggaata agtccaatag
    17761 atgcttattg tgggttaaag gatacattta tttaatattt aaattatata ggagtgattt
    17821 acatgttata tcccattatg acagagacga ggcaagtaat tgatttaagc ggtctttgga
    17881 gatttaagtt agatacaaat gatgacttac aagaagaact tgctttagaa ccaatggttg
    17941 aaaaagagag ttatccaatt tcagttcctt cttcttataa tgatttattt gaaacagaga
    18001 acattagaaa tcatgttgga tgggtgtggt atgaaaaaga agtgattctt cctaaaaaat
    18061 tattagaaga aagattagtt cttcgttttg gatctgcaac acatgaagca aaagtatttt
    18121 tgaatggaaa attgattaat caacacaaag gagggtttac gccatttgaa tcagaaatta
    18181 atcagtttat aaaagatgga agtaacagaa ttacagtggc tgtaaacaat gttattgacg
    18241 atagcacttt acctgtaggg attttgaaag aaacgataca ggaagatgga gacataaaaa
    18301 aagaaaattt ggttaatttt gactttttca attatgcagg cattaataga cccgttaaat
    18361 tgtatacaac tccgaaagac tatataaagg atgtcactat taatacaaaa ataattgatt
    18421 cagatgcaaa tgtagaattt aatatcgata cagaatggac cggagaaatt ctaatagcaa
    18481 tctatgacga agaagctcaa cttatagcag aagctaaggg gaaagaagga gtgattcaac
    18541 ttaagaaccc aacactttgg gagcctctaa atgcttactt atatagtatg aaagttagtt
    18601 taattaacga aaataaagtg attgatacgt acacagaaga atttggcatt cgtagtattc
    18661 aggtttcgaa tgggaagttt ttgattaata ataaaccttt ttactttaag ggatttggga
    18721 agcatgagga cacgtatatt aatggtcgtg gtttgaatga agcagtaaat atcaaagatt
    18781 ttaatttaat gaaatggatc ggtgctaatt catttaggac ttcacattat ccttattcag
    18841 aagaaattat gcgcttagca gatcgagaag gtattgtagt tattgatgaa gtacctgcag
    18901 taggattaca tttgaatttc atggctactt tattagatga caacgtagaa aaacatgata
    18961 cttggaaaga aatacggaca gaaaatgctc acaaaatggc gattaaggaa ttgatagaac
    19021 gcgacaagaa ccatccgagt gtcgtaatgt ggtctattgc aaatgaacca gattcggatt
    19081 ctgaaggcgc taaagaatat tttgaacctt tagttgaatt gactaaagaa cttgaccctc
    19141 agaagagacc tgtaacaatc gttacttact taaagtcaac accagatatt tgtaaagtag
    19201 gagatattgt cgatgtttta tgtttaaatc gctattatgg ttggtatgtt tcaggtggag
    19261 acctaaaaca tgcaaaatat ctgttgaaaa aagagctaga tggatggatg gaaagatgtc
    19321 ccgatacgcc aataatgttt acagaatatg gagcagatac agtagctgga cttcatgata
    19381 cagtaccaac aatgtttaca gaagagtatc aagtgggtta ttacgaagca aatcacgaag
    19441 taatagataa atgtgacaat tttgttggtg aacaagtgtg gaattttgca gattttgaaa
    19501 caagtcaagg gattttaaga gtgcaaggaa ataagaaagg aatctttact agagatagaa
    19561 agcctaaaat gattgctcac tatctaaaga aacgatggtc atcaatacca aatttcagtt
    19621 ataaaaaata atttaccttt tataaggaat ataaaaaagg agtggctgct taattgccaa
    19681 ctcctttttt ttataaaaag attttctgtt taaagaagga tatttttttc tagttttttc
    19741 gaaaaaaagg cgctttctga ttgttgtatt agtggaaagg ggaaaaaagg agcatattta
    19801 tcatgcaaca aaattacaat gagaaatcta tacaacatca atttgatgcc tactgtaaaa
    19861 aagtattacg aaacgaaact aagtctattc gaaaacgaaa cattaacata acagataatg
    19921 aggaaccatt gacgaattca aatgaaggta agtactccca aactcatttt gatgaacaag
    19981 atgtctattt cttatttgga atggaaatat taatttcaga tcaaaagtta tcggcagcca
    20041 ttgatcattt atcggaaata agaagaaagg ttatcctact ttattatttt gcaggtttta
    20101 atgatactga aatcggtaaa atcctcaata tgagtacaag tggtatctgg tatcaacgaa
    20161 agaaggcagt tgaacaatta aaaatggggt atgacttatg gtagacaacc ccaaaaaacg
    20221 gcagtatcca ttagttcctc tatccattat ccaatctgct ctagaagaag atccaatttc
    20281 aatcaagatg atactcaaac attatcatag ttatatagct aagttatgtt tgacaaatgg
    20341 atttaatgag gcggggcaat ttattaccta tgtagatgag tatatgctaa gacagctaga
    20401 aattaaattg atagaagcga tcttaaaatt taaaattaac taactaaaaa gtcccctttc
    20461 cacttattct gtttaaaaga atcttctaat agttaaagga ttagtagatt cttttaaaca
    20521 gaaaacctat tgatcattga aatttaaata cattctatca gtacgttaac acttcaagga
    20581 gcatatttta gttgcgccat gatttcttac tgtaaagaag gagcgataaa cattctaaaa
    20641 taatttttat agtcaataaa aagcgatgat ttgaagttgg gataatgata cttccgtaag
    20701 gctcgccgta aacttggaga gaggtgaaat tcctatgtgc ttttgacaaa agcagctgat
    20761 taagaattat ttccatatgt tgatttcaac attttttcta ttatttttaa gaccaaattc
    20821 agtgaggcaa atagcatcat gtaaatttag tttttctatt gaacgttctc cactttttag
    20881 tttttttata gtactcaaat ttaatcctgt atgtttagag atctcatttg cagtatggtt
    20941 ttgtaaagtt tctcggatta ttgttatatt agcaatttca ttacgcataa ttatgttcct
    21001 ttcttatcaa aaatatcaat gactacagtg ggcttttagt gaaaaaaagg ggtactacag
    21061 tctcttttga aaacaaaaaa atacagtata tacgttttct aaatgctaag caccaacgtt
    21121 tacactgtat ttaagggttt ggcacccaat gggctgtgag gggctcgaac ccgcgacccg
    21181 atgattaaga gtcatctgct ctaccaactg agctaacagc ccaaattaat actttgttat
    21241 tctaacacct aaaataaaaa agtgcaatac ttattatcag aattgagagt gaattttatg
    21301 gattttcaag attatgaaaa aatatttgaa agttatccat cgatattgaa aattactgat
    21361 ttaactaaaa tgttaaatat tggaaaaaat aaagcttata atttgctgaa agatagatca
    21421 atagaaaata taaaaattgg agcaaggtac aaaataccta aaaaagctgt cattgagtat
    21481 atattgaaaa tacaagatga aggtaaaaat catgctataa taaaatagtc aaaatttttg
    21541 ccgatatttc agtttaagag gagagattga attgaaatta agagcaaaca taattgaaaa
    21601 aagaggaaca ttacaagtag ttgtatctta taaagactct aacggaaaaa ttagacaaaa
    21661 gtggaaagat acaaaattaa aaaagaaagg aaataaaaaa cgagcagaaa aaattagaga
    21721 tgaattttta ctagaattgg aaagtcaatt agctatggaa tgtgaatgtg ctaatccaaa
    21781 tttattgttt tatgactatt tgcttaatta tttggaagta tcaaaaaaac aagtagcatt
    21841 taacacatat gttgggtata agcattacgt ccataatcga atttacaagt ttttttatcc
    21901 taaaaaaatt aaactaaata ggttaaagcc ttatcattta caagagtttt atcaatatat
    21961 gttaaatgat aactgtacag caaatacggt tattcattat cacgcattta tacgcaaagc
    22021 tcttcaagag gctgttatta cagaattgat aagtacaaat gtagcagata aagtaagaaa
    22081 gccaaaaaag aaacaatttg tttctcaagt ttataatcaa caagagatct tgaaattact
    22141 tgacattttg tcagaagaaa aactctattt ggtagttctt ttaacagcat tttatggatt
    22201 gcgaagaagt gaagttttag gattaaaatg gtctgctatt gattttttaa acaaaaaaat
    22261 catgataaac catgtaatca tagaaaatcc agagaatatg tcacagttaa taaagaaaga
    22321 tcaaacaaag aactcttcca gctatcgtac attacctttg gttgaaacga ttgaacgagc
    22381 attagtaaat caagctaaat ggcaacaaga taatcgtatt cttttaggag atgattatat
    22441 tttagaagat aatgagtacg tatttactat ggaagatgga cggttgatga agcctcaata
    22501 tgtaacgcat cgtttgagta agttgattaa gaaaaatgga ttgaaaaaaa ttcgctttca
    22561 tgacttgcgt cattctaatg cttcaataat gttagatagt ggccagaata tgaaatccat
    22621 tcaagaatgg ctgggacatg catcgtattc gacaactgct aatttataca cgcatttaac
    22681 cgcaggagta aaagatcgtg cagcagaagc attagaaaac gcatttgggt ttaaagaata
    22741 aaaaaacaaa aaacaatatc tttggggtat cgcttcttgt ttttagaatt tttttgttgg
    22801 caatttgttg gcagtatgtt ggcaatgaaa aaattcacaa actaaaaacc tatttataaa
    22861 gctttataaa acttttttca caaatagagc atttggttaa gagtcagctg ctctaccaac
    22921 tgagctaacg gcccgtacaa aaagtactta ataatagtag cactaagaat tttaaaatgc
    22981 aagactacat ttaaagaaaa tcagaaaaat ctttacttcc ttataggaaa tcattcatag
    23041 attattcaca tttgctcgct attatataag cataccaaca aacaaagttc ttgcaagaga
    23101 acaaacccta acactttttt cattttttac tcctttcccg ccgtctcccc ggcgggtctc
    23161 ctgttcttaa cgataaaaga caagaaggct tagccttctt gttttttgtt ttcatggaat
    23221 cccgctaatc aaatcaccta gttaaagcaa ctagattgaa tgtttatcat aagtctgata
    23281 tcatgtttat gaaatgaaaa aaggagcggt tatgatggaa aaaatgcgtc agcaattaat
    23341 ggagtggggg aaggaactag aaacattccg tctgcctcac tgggaagaac tccctgactt
    23401 agaactttat atggaccaag tcagaacgtt agttgatcgt tatctttcac ctgtgataca
    23461 aggagaaaag catccattgc ttacctcttc tatggtaaac aattacgtta aactaggttt
    23521 gataccagca ccagttaaaa aaagatataa caaagaacac gtagcatttt tacttgcaat
    23581 cactacgttg aaacaagtat tgactattcc tgaaatcaaa gaagggatat tgttccaagg
    23641 aaaaacagta ggaattcgtg aagcctataa tctattttgc gatgaacaag aagcagctgt
    23701 gtggatggtc agccaattag cacaaggtaa atctcatccg caaaaattcg accaggcaac
    23761 ccctgtagaa tatattgctg taagagcggc aactttatcg tttgcaatga aacttcttgc
    23821 ggaaaaaacg attgttctag aaaccgaata tttaaaagag gagaaaacaa atgaaaaaca
    23881 ataaaatcgc aattttagta gattcaggta cggatgttcc agaagaattg ataaaaaaat
    23941 acgagatgta tatgatccca ttgaaaatta tttataaaga tcaggtatat acagataaaa
    24001 ttgacattac ccctgaagag atctatcaac gtttgccaga ggaaatccct ggtacatctt
    24061 tgccggatgg tgagtcgatc acagaaatct ttgagcaaat caaaaaagac ggttatgaaa
    24121 aggtacttgc ggtaacaatt tccagtggat tgagcgggac atataatgtg gttcgtttaa
    24181 ttgcacaaca gcaagaagaa ttggaagcat atgtactaga tacgaaaaat attggtattg
    24241 gtgcaggttt ttctgcgatc caagcagcaa aatggctgga agaaggagta gaatgggatc
    24301 aactgatcag taatctgaat gagttagtcg aaagaacaaa agttttcttt aatgttgcga
    24361 cattggaata tcttcaaaaa ggtggacgga tcggtttagt tgcttctatt gtaggaaccg
    24421 ctttaaagct taacccaatc atctcatgta atgaagaagg gatttattac acagtaggaa
    24481 aagctagggg acgtaaaaaa agtttggata aaacggttgc ttacgtcaaa gaaaaaatcg
    24541 gtaatgcaaa ggtcttcaac ttagctgttg cccatgggga tgcgaaagaa gaagcaattg
    24601 aaatggaagc gagacttaaa agagagttcc cacaagcaaa ccaaatctac tttggccaga
    24661 tttctccggc attagtagtc cacactggtc cgggattatt gggtgtcggt gtacaagttt
    24721 tagaaggata aatcaaaaaa aggaaacact gaggtgaaaa tcagtgtttt ccttttttat
    24781 tgaagttaga acaaaaaata ataaagtatt ttgcactttc attttaattt tctcttttgt
    24841 tttataggca aagatgaaac aaaagcagaa atacggtata atgagaaggt atgacaataa
    24901 tatataagag gtggctcttt atgaaaaaag ttttagttgt cgatgatgag aagccgattt
    24961 cggatatcgt caaattcaat ttagccaaag aaggctatga cgtttatact gcatatgatg
    25021 gggaagaagc cttggaaaaa gttgctgagg tagaacctga tttgatttta cttgatttga
    25081 tgctacctaa aatggacggc ttagaagttg ctcgtgaggt acgcaaaact tatgatatgc
    25141 cgatcattat ggtaacagcc aaagattctg aaattgataa agtcctaggc ttagagttag
    25201 gcgccgatga ttacgtgacc aagccatttt ccaaccgaga attagttgca cgtgtcaaag
    25261 caaatcttcg tcgcggtgcc acggctgcga aggaaccaga agaggcagca cctgcggaat
    25321 tgacgattgg tgatttgacg atccatccag aagcttacat ggtgacgaaa cgaggagaaa
    25381 caatcgagtt aacacatcgt gaattcgaac tactctttta cttggcgaag caccttggac
    25441 aagtgatgac aagagaacat ttattacaaa cggtttgggg ttatgattat tttggagatg
    25501 tacgaacagt ggatgtaact gtacgtcgtt tgcgtgaaaa gatcgaagat aatccaagcc
    25561 atccaaatta tttagtgaca cgtcgtggtg tcggttacta tttgcgtaac ccagaacagg
    25621 agtaattgga tgaaaagaaa agtccgcttt tttcgatcag tcaattttaa gatcgctatt
    25681 acctttattc tgattttatt gatctcgatc gaaattattg gtgcctactt tatacgtggt
    25741 ttagaacgtt ccacgatcaa tacatttatt aaagatatga atcaaacggt ggaatcatta
    25801 gcaacaacaa tcagcccgga attgaatcga aaagataatg cagatgatga agaggtaaat
    25861 gcgaatatca aaagatttat tgaaaacagt gcgactagcg atatcatcga aatacgggtc
    25921 gttgacgaaa aagggatcat ccgaggaaca accgatgtca atgaacaaag cgctgtggga
    25981 aagaaaaatg attatgttga tatcaatgat ttcacgacta aacgttatgt tgctttagat
    26041 aacgacgata aacgggtgaa tatcaatgtt caaccgatcc tgtccccgac cggcgatgcc
    26101 gttatcgggg ctctttacgt caaaagtaat atcgaacaaa aatattcgga aatcaataac
    26161 acagcagtca ttttctttac tgcctcacta attgcggcat ttatttcaat gatcgtatct
    26221 gttttagttg ctcgttcgat tacacagccg atcggtgaaa tgagagaaca agccattcga
    26281 atcgctagag gagactacag tcggaaagtc aaagtgcatg gacaagatga acttggacag
    26341 ctggctgata ccttcaatca gttagctgaa cgtattgaag aagctcaaga tacaatggaa
    26401 gctgaacgca atcgtttgga tagcgtgcta tcccatatga cagacggcgt cattgcgaca
    26461 gatcgcaggg gtaaagtcat tacgatcaat gatatggctg tttccttgct agatatcaaa
    26521 aacgaggaag caatcggaca atcgattttg acacttttag atatcgaaga agaatatacg
    26581 ttgcggaaat tattagaaga tccaaacgaa atggtacttg accgttcaac cagtacattg
    26641 gaaagtgatc agatgatctt gcgagtagac ttttctatga tccgtcgcga atcaggtttt
    26701 atcagcggat tagtagctgt cttgcatgat gtgacagaac aagaaaaaac agaacgggaa
    26761 cgtcgtgaat tcgtctctaa tgtttcccat gaacttcgta caccgttgac aagtatgcgt
    26821 agttatatcg aagcattgag cgaaggtgcg tggaaagacg aggaagtagc accaaatttt
    26881 ttgaaggtaa cgctagatga aacagaccga atgattcgaa tgatcaatga cctcttaaat
    26941 ctatctcgca tggatacagg taatacccag cttcaattag aatatgtcaa tttcaacgag
    27001 ttagtgaatt ttgttcttga ccggtttgat atgatggtgg gaaatcagga aaaaaattat
    27061 aagatccgtc gtgaatttac gcaaagagat ctttgggtcg aagtagatac agataaaatc
    27121 atccaagtag tcgataatat catgaataac gcgatcaagt attcaccaga tgggggaacg
    27181 attacttgtc gcttattaga aacgcataat aacgtgattt taagtatcac cgatcaagga
    27241 ttggggattc ctaaaaaaga tctgaaccga gtgttcgagc ggttctatcg agtagataaa
    27301 gcacgtgcaa gagcacaagg tgggacagga cttggattag ctatttctag agaagtgatt
    27361 aaggcgcatc gaggcgctat ttgggcagag agcaaggaag gaaaagggtc gactttttat
    27421 atctcgctgc catatgaacc ttatgaggag gaatggtggg aatgagaatc tctgaaaaag
    27481 tcgttcgtat aggactcatt ttgatggttg cgttaagcat atacttctct tatgcaatct
    27541 ggctgagccc agcaggaaaa agttcaatca acgtagatga gacaaacact caaatgatcg
    27601 agtctcaaag caatcgaaaa acttccgaag tctttcttcc gcttcatgtt acatggcacc
    27661 attcgggaat gatccaagaa accaacagtg aaaatctcgt gtaccgattg caattgatca
    27721 tcgataaagc aagatttgga aagctttctg aagtagtttc aggagacaaa tcacagtttg
    27781 aacaggtgaa aaatctgaac tctggttttg agttggccta taatgcgcct tttttgctaa
    27841 gagaatacaa ggaagcattt gatttagatt tagatgtgtc gaacttggca aaagaaagcg
    27901 aaaatatgta ttttactaga atacaattcg atcaaggaaa taaaaaaatc cgatttttga
    27961 attattcgaa ccatctcgta tatgaagcaa atatttcgtt gaattggaca gatatcaata
    28021 aagaattaac agcaaaagac acaaaatgga cagaaatgac aacagaacca tcgacgatcg
    28081 acacgcaata tgctacgaaa cattcgatca aactgaagaa gtatagttat atcctgtcac
    28141 agcaacccta tacacttttt agaaatgctt ttttccagaa accagatagc gtgaaaaata
    28201 atgatgaaag caatgaattg attttttacg gtgggaatga aacaatgtct atccattcgg
    28261 aaacgcaagt agttgatttt cgtggagagc tgccagagaa aaaagaaaat gctagtgtat
    28321 tttctgaaag tttcccctat attagcaaat taggcagttc tttagggaac ctcagatatt
    28381 ttgaccgcag tgacaatacg atcgattatc ggatattcgt ggaaggcttc ccggtttttg
    28441 ggacagacag cagaggacag attggtgtgg aagttggcgg tgaaacgaat ggttctgtgc
    28501 aagtgaatat ccaaaccagt ttgaatacga ttcaagtgcc gataccttca gacgaagaag
    28561 tagaacttgc aagtacgaat gaaatcatcc agcagttaaa agctaaggga gcagatagca
    28621 acaagattgg ttcagtgatc gttggctata catggcaaaa tgtcaaagaa acgaatcgtg
    28681 tcgttgatct cttacctgaa tggtatgtga aatataaaga taactggtat tcagctaacg
    28741 accttctgga aaaattacct ggattggagg cgaattaatt ggatttcaaa aaaatcgaat
    28801 ggatattttt cgtcgctttt ttagggttga atgtgttttt attcagcagt tatcatgacg
    28861 cggtatacca agaaaacaat gtcattcgct ctaatcagac tgaatcaatc caacaacgtc
    28921 tggctagcga cgatatcact tacacgggga agatttcgtc tgagaacaag caaggctatt
    28981 atcttagcgc ggagcagacc aatcttagca cttcattagc gaattatcgc aaggctggta
    29041 aaagcggctt gctggcaagt ggtgtgtttt tagacggtga tgttttgacg aaatctttat
    29101 ccagttcaga tgctgaaaag aacgtgatcg atccagataa aattagtagt tccttggaag
    29161 attttttatc taacaaagac aatattttat tcggtaatga ttatgtgtac acgaaaaact
    29221 tttctcattt agaagaagaa ccactagaaa tccaagcaag ccaagagtat gaaggcatcc
    29281 cattcaatga tgatacagca aaaatcgttc tttctttaga aaaaaacgat aatgattata
    29341 gcatcacgaa atacatgcag acgcatttat ctgatatcga gcagctgcgc gaaaagacgg
    29401 aactgtatac ggaagaggaa gcaatcaata cattgtacat caataacaaa ataccaagag
    29461 gatcaaaaat tttatggcgt cagctagctt attcctgtat tctgaaagtc cgggaaaaaa
    29521 atgtgtatgt tcctgtttgg catgtagcga tcgaaacaag cgataaagtg gtccaaattg
    29581 agagcgtcaa cgctttcagc aatacgatcg tgacaaacaa tactgtacca aaagtggaag
    29641 atcaataaaa aagcgagtat aatgaaaaaa gtttacaaag taagaaggga agtaggagtc
    29701 aaatggctga agaaaaacca gcttttaaaa tcagtatcct agcaagtgga agttcgggta
    29761 attcacttta tatcgaaagt gaaaaaaaac ggcttttggt cgatgcagga cttagcggta
    29821 aaaaaatcac ttccttgatg gctcaaattg atcggacacc ggaccaatta gatggaatac
    29881 ttgtgacgca tgaacatcga gatcatatac atggcgtggg tgtcttggct cgtaagtatc
    29941 atttggatgt ttttgccaat cagcagactt gggaagcgat ggaaccactg attggaaatg
    30001 taccagtgga acaaaaacat ctatttgaga tggggaaagt cagaacattc ggcgatatcg
    30061 atattgagag tttcggcgtc tcccatgacg cagcggctcc gcaattttat cgtttccata
    30121 aagaaaatcg gtcattcgtc atgttgacgg acaccggata ttgcagtgat tatatgcgag
    30181 gtgtcattga aaatgccgat ggttatttga tggaaagtaa tcatgatctt gagatgttac
    30241 gcatgggacc ttatccgtgg aatttaaaac agcgtatttt aggagataga ggacatcttt
    30301 ccaacgaaga cggtgcactt gtcatgactg atgtgatcgg taatcggacc aaacgaatct
    30361 atcttggcca tcttagcaag gaaaacaata tgaaagaact tgctcattta acaatggaaa
    30421 atgttttgaa agaaaaagat tttggcgttg gacatgattt tgaaatctat gatacagatc
    30481 cagatgaagc aactgagcta ttttcgattt aaacagttca atcaataaaa atataaaaag
    30541 cagaagataa aatcaatcag ggttggtttt atcttctatt tttatttttt tagcatatta
    30601 aaaaaataac cgtattatcg actgactaca ctgatgtgct gcttttcatg tttctcatcc
    30661 agtatctcat ctactaatgc aatcgcgtaa tctgccatgc tgatcgtact gtcgctagca
    30721 gcgtttcttt gtaaataatc atcacttaac aaataagtac ctgtacgttt gccatgtgga
    30781 tcaaataaag aagctggact gatataagtc caatgtagat ttttagattg tttcaatttt
    30841 aacaatcctt cgtacatatt gaaagctgtt ggatagaagg gtgcattttt aggtacttgg
    30901 tcaatcatac gagtcgtctt ttctgtatct aagaataacg aagaggcacc cccaacgaca
    30961 attaagcgtg tatcggttcc ttcaagcatc tcaatcaaat gggacaaaga agtttgatgc
    31021 atttcttctt ttcctttggg agcgttgaaa gcatcaacga caacatcaaa agatgcaaga
    31081 tcagtagttg tcaactcaaa taaatccttt tgtataaacg gaacatctga tggcaatttt
    31141 tctggatgac gcacgattgc cgtcacgtct aaatctctgg acaatgcttc gtctaaaata
    31201 aatgaacctg catggccagt agctccaatg agtgcaattt tcataaaaaa cctccttttt
    31261 tattcctgtc cattgtaagc agttgctgaa tattcgtcaa agaatccgat ttttgaaaag
    31321 ctcagtgcgc attcctttca tggttcgtga tcattgggta tagttaaatt aagaaaactg
    31381 attgataaaa atagaagttg aaggagataa gaaatgaaag agtggttaga tcgtgccttt
    31441 ttaccaatga taacatctgt tgtgaatctt agacccttaa ctgctttgcg taatggaatg
    31501 acttacacga tcccaataat tattgttggc tcgctgtttt tcttatttgc tcattttcct
    31561 gtggattaca tcgctcagtg gatagacaaa acaggatttt ctatgtattt tacacaagta
    31621 taccaggcta catttgatgt gatagccttt tggatcgttt ttgccattgc atacgcttat
    31681 gcaaaagaag aagacatcga tcgcttacca gtcggcataa cagccttgtc tgcttatcta
    31741 ctgcttattc agccactaaa tactcagata gatactgtct ggacaggtgc tgaagggata
    31801 ctaggagcag ttacaactgg gctcatcatc ggatggggct atgcacgttt agtaagactt
    31861 gcgaaagggt ggaagcaaag agagaagata cctgaaaatg tttggcagtc ttttacttca
    31921 ttgattccaa tcattgttat agtcactttt acgcttatcg gttcagcggt ttttcaacat
    31981 ttgttcagaa tgactgttgt tcaaggagta tggacatatg tccagcttcc cattcaatcg
    32041 ttcatagaca cgttgtttgg tgtgctttta ttaggttttt ttgttccgtt tttatggtta
    32101 tttggtcttc atggatctgc gatggtcaat gggttggtta gtcccgtctt gcaagcgaat
    32161 tcattagcaa atgcagaaat cttagcctca ggacaagagt tgacggtggc aaatggcgga
    32221 catatcgtta ctcagcaatt tctcgatcag ttcatgacgg ttacgggtgc tggattgacg
    32281 ttaggggctg tattttttat gacggttttt gcgaaatcga agaaatacag agaattagga
    32341 aatcttgctc ttttaccagc ctttttcaac atcaatgaat caattatttt ttccacaccg
    32401 atcgtgatga acccgatgat ggctgttcct tttattttcg caccggtcgt ttcaggattg
    32461 attacctatt ccgctttata ttttggattc gttcctttat ttacagcagt ccaagtccct
    32521 tggacaacac cgccgatcct ctcgggtttc ttagtaggcg gagtgcctgc agcgatttta
    32581 caaggtatcg tgttgtcaat tagttttttc atttatttcc catttttgaa aaaaatagat
    32641 tctgcaaact gcaaaaagga aagacagaca aaaaatgatg gagcagcaga aaagatgaac
    32701 gactaaaaaa caaaaatact cctgtcgctt tagattggga aaatcaccaa tctaggatga
    32761 caggagtatt tatagtcatg tttatactag ccctttttgg acagctaatt gaacaagaac
    32821 aactaatgtg ttcattccta cgtgtgtgat catcgatgtc cagatccgac cagttgcttt
    32881 gtactctagg ctgaagaaga aacctaggaa aaagtagatt agcagatgac catcattatg
    32941 agcaaacgca aacagcaaag agctgatcac tgctccgacc caaaaatttg aatatcgact
    33001 gatgatcccc agtatactgc ggcggaatac gaactcttcc atgatgggtc cagcaacagt
    33061 tgtcgccaaa ataaaagcgg gtgcttccat gatcatctgg atgatatttt gtgtattctc
    33121 ggaaggggtt gctttgccaa acaatacaga ttctaacatg acagcgatgc tttgcagaat
    33181 caatgcgaca aagattccaa ccaatccata aataacaatc tttggagcag aagctttttt
    33241 taccgctgat tcgatcgcta agggttcacg ttgcttaaaa taaaaaatag ctaggacaac
    33301 ggcgcctagt atatacatga ctgcagttgc tgtgatcaat tcagacgtag attttacaag
    33361 tcctatcgaa gctaggatca agggtgagat aaaaattaga ccatagcaaa aaatgctgag
    33421 gaagctatat ttttttaaag acatgtaatt cctcctttta agggataaca agcatgcaag
    33481 gcaagcttat atcgattgat ttcaattata tcataatttc aataagcagc tgtcagaata
    33541 aaatttcttt tctttgattt aaaagcttgc aaaaaagagg gagaatgagt attattaaaa
    33601 atgtaggtta gcactcgaag gttaagagtg ctaacagatt aaaaaaagag ttatcggagg
    33661 gatttatcgt gttaaaacca ttaggtgatc gcgtcattat cgaagtcgca aaagaagaag
    33721 aaaaaactgt cggaggaatc gttttagctt ctgctgctaa agagaaaccc caaactggga
    33781 cagttgttgc agtcggtgaa ggccgtttgt tagaaaacgg tgaaaaagtt cctgccgctg
    33841 taaaagcagg cgaccaagtt atgttcgaaa aatacgccgg tacagaagta aaatacgaag
    33901 gaaaagaata tctgatcgtc gcaggaaaag atatcatggc gattgttgag taaatttgga
    33961 agataaaaaa ataatcaaaa ataatcaatg aggtgaatca atcatggcaa aagaattgaa
    34021 atttgcagaa gacgcacgcg cagctatgtt acgcggggta gataaattag cagatacagt
    34081 gaaagtaaca ttaggaccta aaggccgaaa tgtcgtacta gaaaaatctt acggctctcc
    34141 attgatcaca aatgatggtg tgacgatcgc aaaagaaatc gaattagaag atcattttga
    34201 aaacatgggt gcaaaacttg tatcagaagt agcatcaaaa acaaatgata ttgcaggaga
    34261 cggtacaacg acagcaactg ttctgacaca agcaatcgtt cgcgaaggtt taaaaaacgt
    34321 aactgcaggt gctaatcctc taggcattcg tcgcgggatc gaattggcaa caaaagcagc
    34381 agtcgaagaa ttgcataata tctcaactgt tgttgattca aaagaagcca ttgcacaagt
    34441 agcagctgtt tcttcgggtt ctgataaagt tggacatttg attgctgatg caatggaaaa
    34501 agttggaaac gacggtgtca tcacaatcga agaatcaaaa gggatcgaaa cagaactaga
    34561 tgttgtagaa ggtatgcagt ttgatcgtgg ttacttatca caatatatgg taacagataa
    34621 cgacaaaatg gaagctgttc tagaaaaccc atatatcttg atcacagaca aaaaaatctc
    34681 taacatccaa gatattctgc cattgttaga acaaatcttg caacaatcac gtccattgtt
    34741 gattattgct gacgatgtag atggtgaagc attgccaact cttgtattga acaaaatccg
    34801 tggaacattc aacgtagtag ctgtaaaagc tcctggtttc ggcgatcgtc gtaaagcaat
    34861 gcttgaagat atcgctattt tgacaggtgg tacagtaatt actgacgatc taggtttaga
    34921 attgaaagat gcaacaatcg aaaacttagg aaatgcttct aaagttgttg tagataaaga
    34981 caatacaacg atcgtagaag gttctggaga aaaagaagca atcgaagctc gtgtgcaatt
    35041 gatcaaaaac caaattgctg aaacaacttc tgattttgac cgcgaaaaat tacaagaacg
    35101 cttagctaaa ttagcaggtg gtgtagctgt tgttaaagtt ggtgcagcta ctgaaacaga
    35161 attgaaagaa ttaaaattac gcattgaaga tgcattgaat gctacacgtg ccgcagtaga
    35221 agaaggaatg gtctcaggtg gtggtacagc tctagtgaac gttatcagca aagtttctgc
    35281 tgtagaagca gaaggagacg tggcaacagg tatcaagatc gttgttcgtg ctttagaaga
    35341 accaattcgt caaatcgctg aaaacgctgg ttatgaagga tcagtgattg ttgataaatt
    35401 gaagaacgtg gaactaggca ctggtttcaa tgctgctact ggagaatggg taaacatggt
    35461 tgaagcaggt atcgtagacc caactaaagt aacacgttct gccttgcaaa atgccgcttc
    35521 cgtttctgcc ttgttattaa cgacagaagc agtcgttgca gataaaccag aaccagcagc
    35581 gccagcagct cctgcaatgg atccatcaat gatgggcggt atgatgtagt aattaagaaa
    35641 gaaccctatt ttaacagggt tctttcttct ttttttgttt tattttaagc aaaaggggca
    35701 aagaaggggc aaaaatatca aaggctattt agtttatcaa ttacttgttg tttggctctt
    35761 tttgttacat ggttataaat agacagagtt gtgtttgcat cagaatgccc tacacgctcc
    35821 ataatggctt taagaggtac gcctaattca gataatagtg aaacgtggct atgtctgaat
    35881 atatgtgagg atagactttt ttctaattcc aactcttcct ctactttatg gagtatagcg
    35941 ttaaatgagt gcagcgcaag cggagtgcca cttgtagata taaatatata ttgatcggga
    36001 tctgtgggtc tacctgcaag aatattgtca gctatcacac tttcaatcaa ttcttttgca
    36061 cgattgggta attgcacttc acgttgcgaa taagtatttt ttggagttgt ttttatagca
    36121 ttatccattt tcacagatgt gtaatctaag gtcccattaa tggaaatttt cccatcctca
    36181 tagtccttca tttgcaaagc tagcaattcc ccatatctca aaccagttaa atatagaaac
    36241 tcagctatta tgccgtgtag ttttctgcga ggattggagt atagctgttt cagtatttga
    36301 tcaatttctt ctttctccag atatttttta ttcatagaaa gccttctttt ttcttcttcc
    36361 acttttttag gatggatttt aactgctagc gcagggtttc tttgaatgta ttttctatcg
    36421 attgcatagt ttagcataac agatagagtt gtttttgttt gttttgtgta gttcagtgag
    36481 aggtcaccaa acgtatacat atcttcaatt atcttattaa tgagtgtctc atcaatgttt
    36541 ctaacgatcg tatcatcgct tatgtgctta gaaacatgtt tcatcatcat tggaaccttc
    36601 aaatagctag tacgtttaac atgctgctta taatattcat accattcttt atacagctca
    36661 ccaaaagtga tatctgattt attgtaatct tcgagtgctt ctttaatttt tttatctaga
    36721 attttctgag cttttttcca cgcttgtggt gaattacttg taagtgttgt agattttttt
    36781 cgtgtttttt ctgtataagg atctacatat ctttcaataa acttgaattg gccatctttg
    36841 gtctgttcaa cccacacttt taacatctcc tatcatttgc tataataggc ataacaaata
    36901 gacctatata ggtttgtttt ctaaaagcac gctcttactc tggacggtgg ggcgtgtttt
    36961 ttatttatta tacagttgct ttaaaaatag ctgctttttc aaatgatact aaaggagaga
    37021 agtggatttc tatttctccc ttttggttta acgtgaaatg agcagttaca tccatttgtt
    37081 ttcctggagc aacagatccc attgtatttt cgttggcata tgtctctgat tttttgtcat
    37141 ctggtccata tacttccaca tctgtaccta cagggatatc tgaatcgcca tcatttttta
    37201 cggtataagt aatttttact acttgtgcag gttggttttc ttcaaattga tttctttcat
    37261 cagttagttc tacactattt agtgtatatt cagcatcacc aacttttaca gtgtcaccaa
    37321 tcttatagaa ggtatcgctt ttttcttctt tagaagatga agcagaggtt gaagatttag
    37381 ttactttttc gccaccatta tcacttgctt tattactatc tgatccacca tttaaagcag
    37441 aaccaataat tataattaaa attactgcta atacccaaaa ccatactttt ttgtaaaaag
    37501 gtttactaac tttatacatt tttccgtctt gacccataac tttttttgcc atttaaatat
    37561 tcctcacttc ttgttataat atatttgcga tctcagaaat gaggtatgag tccgtgtgcc
    37621 agcacgggct tttttaatgt tttgctgaaa tcggtttttt taataattct tgatgtattc
    37681 atacatattg ccttgcgtaa gaatattttt tcttaaaata gcattggcag acagcataac
    37741 aagtgtatct gagcttattc tgaatagaat aatactccat aaatttttcg agattaaact
    37801 gagattcatc agtcagttcg ttctcaatat agatatttaa taaaattaga atagctattt
    37861 tatctgcttc tgtttcaaat tttgagtgaa aagttgtaga agtatcgtac aaaactgaat
    37921 attcaaaatg agaagcggtg aaatgcgcaa gttcatgaga taaatgaaag gcttctgcag
    37981 tttcgccata tagattttca ttcaaaaaaa tgattctagg cttcggataa tagaagccag
    38041 gctctttcat ttccatgtaa actactttca aattgtattc gctcaacatt tctttcaatt
    38101 tcaaatacat acaacccatc actccaacta ttcattttcc tctaaagctt tagcaattgc
    38161 aatcgcttta cgcattgtct ccttagatat ttcttttcca tcaaaagaaa aaacagtatc
    38221 gttttctgat aaatccacat gtttaggggt ttctctttct tctctaccta gaaggtagtc
    38281 tacagagact ccaaagtaat cagctaaccg agctaactca atagaattag gagtttgtct
    38341 tttccaactt cctaaatatc cattcgaata cccgaaattc atttcgagct gtctaatgga
    38401 taatcctttt tcttttgcta actcctttat tatttcgtaa gtattcattg ataaatcaac
    38461 ctttctgaat gcttacaaaa aaagtttaga taaataatgt aaaaatagtt gacttatata
    38521 cattattaat ctatactatg ttttgtaaac aagtttaaca actaaaaaga caacaaaaaa
    38581 tactattgat taataaatgc caaccgccaa gaaagctttt taaatcaaag tttatatgtc
    38641 ttatttaact atgcatttag tatagattat taatctaata aagtcaacgc agttttttaa
    38701 aaagttgtta aatttgttta cgaatataaa agaaaggaga gaagaatatg gaaaaaacag
    38761 tctcggcaaa aatcgaagat ttgaaattag acatactgaa acaagcaaaa gtggcgatgg
    38821 aacacgcggt agataaagaa gactctgcca tggttgcagc catagcagag attttagctc
    38881 acgtttagtc atcgttttta cggtcttctg gaagctgacc atagataaca gcataatgaa
    38941 tatctaaata agcttgaaca atttcttttg gcgaaattga ttcgccttta gtagatactt
    39001 gtgattcgtg atttagccat gcaacaacta aatccgcagc gatttcaaac ggcgttacac
    39061 tttgtgtcag ttgccataaa gaatttcaca atatgtatgg tcgtggtaat aataccaaag
    39121 agcaatttga ggaatttatg gaaattaaaa aatacaaaaa atcatttatt aaaggaggct
    39181 aaacaatgaa caaggaaatg agccaaacaa tcaaggtaca aaaaatgata gatgatctaa
    39241 cacatggaat tgatagccaa gccgataaaa tcatcaagga attgcaagga caaaaagtta
    39301 aggatgcaaa aatgcttctc aagactatca actttgaaat gaatccaaca aaaagaaagc
    39361 tcgccgatgt gttggaagaa aagttggctt ccgctatcaa cgagcaagaa ttactatttg
    39421 aaaccgatac ttttaacagc tgatgtttta tagatgacag tttcatccga atctatatca
    39481 tcgccgagag aaaagaatgt tgcttttgac aaaaagccga taagacctac aagcggtctt
    39541 gatgttgcaa gagtagatac cccaatatca ccttgcaagg tattcacgta aatttgagaa
    39601 gaatagaaca ttccatcttt ttcttcgata tttgacgcat catcgtatcc aacgaccaaa
    39661 gtatcggcaa atattttgat tttttctcca tcaagcatag taattacaaa ttctggtttc
    39721 ataaattttc acctccttaa caattatttc agcctgtcac actgataagg aaattatacc
    39781 aaagaaagga atgagaaata tgaatacacc acaaattttt aatttcgagc aaaacgaagt
    39841 tcgaactatt ttagtaaatg atgaaccata ttttgtagga aaagatgtag cagacgttct
    39901 aggatattcg aatccgcaaa aagctattcg agatcatgtg gacttggagg ataagacgca
    39961 gaacgattcg ttcaccgtca atggaacagc agttgttttg attaacgagt caggccttta
    40021 cagtttaatc ttaaaatcaa aacttccctc tgccaaaaaa tttaaacgtt gggtaacgag
    40081 tgaagtcctt ccaacaatta gaaaaacagg tagctattca aacgtacctc aaagttttgc
    40141 acaagcattg cgtttagcag cggatttaga agaaaagaac caattactcg aacaacaaat
    40201 tgccgagtac gaaccaaaga ttagctactt agatacgatt ctttcatcga cagatacggt
    40261 agctacttct caaattgcag ctgattacgg aatgtcggca attgctctaa acaaattgct
    40321 aaacgagtta ggtgttcagc ataaagttag cggacaatgg atactttacc gaaaacatat
    40381 gaaccaagga tacacaaaat cgcacacaag tgagataccg aaagccgatg gcggcactaa
    40441 agttgtaatg aataccaaat ggacacagaa agggcgagtg tttatttaca acttattaat
    40501 cgcagagggc tattaccctc aaatggattt agaggaaatt ggttagaaag gagttttagt
    40561 atgactgaca ttgcagaaat cactcaacga gatagagaaa aaatcaaaga atatgtcgaa
    40621 agttcaaagt tcttaactta caccatgctt gctgaaagat ttggaattag caaaagctac
    40681 ttatctttaa ttttaaacgg taaaaagact tctgcagaag caaacagaat tatagattcg
    40741 attatcacta tgtacgaatt gtagagggag gtaaagtcgt tggaagataa aatcattctt
    40801 acaaaatcag agcttcaaga aatgttaaac atcgagtatg gaacgataat ttttaggagg
    40861 gataataagt gaaaaaacca acactttcag agctgataga agctgctgag aaggcagtaa
    40921 aaccagacga ctggtaccga caaagtttaa tcttggagaa gttccacggc atgtcaaaaa
    40981 ctactttagt tgaatactgc aaggaaatgg aaacaattcc tgaatttgca gaaggaatac
    41041 ttcgtccagg tcactcaacc acatttattc attaccatac ttttatttgg tttttaaagt
    41101 ggaaagacgc aaacaaatat cgtgtaaaaa gtttatcgcc ttcagatgtt ttgaaggagg
    41161 caagttaatg ggcaaattca acagagcact agtattcagc gcaccgctaa tcatctacgc
    41221 tttaggcctc tggggaagca gacaagcgtt gatagggaca atcgtttaca tggtttggat
    41281 ttttatgggg cttgatgaag ctgagcacag agcgaaaaaa gccagtcggg agggactgac
    41341 taaaatatga tttttttatt tttgttcctt tgggctgcta ttgcgtactt catccagctc
    41401 cttgttgtca aagttgctct tatttctttt aggaagaaca aaagaaaata gcgctataaa
    41461 tgaaagataa gtagccgcta cattaaactg ttcttttgaa aaagttacag tcatcggaat
    41521 gataattgat attgaggaag taatcttaat taaaaatata gcaatatttt caacatcgcg
    41581 ttggttttta tttaacattg tccaacatgt tttagcaaca ataaaaataa ttacacatga
    41641 tattagcaca cagactgaaa aaagttttag aaatgcgatg tagtcagtgt tattgaactc
    41701 cttcaaaata ctatttttat tcaatgtaat aaaaggaatt tttttaattg aagacaggaa
    41761 agataaggta ataaacagaa ttatatatat ggttaaagtc gcagtaagaa tgtaattgag
    41821 ctttttcctc atagaatcac ctcactttct aacgaaaatt ataccaaaaa ggagagaaga
    41881 aataatgcaa gaattagtaa ttttgaaaaa taaagaagct gtaactacga gcttacaagt
    41941 ggcagaaagt ttcaaaaaga aacataagca tgtactagaa gcaattgaat caataaaaag
    42001 atcggtcgaa aattcggcca atgttgaaga tgggtccaat tttggacaga tgtttgtgga
    42061 agggaacgag ccagactcat acggaagaag tcggagagtt tatttcatga atagagacgg
    42121 attttccttg ctagctatgg gattcactgg aagtaaagca ataaatttca aactaaaatt
    42181 tattgaagct ttcaacgaaa tggaagatgt tattcggaag aatactgttc ctcaaacaat
    42241 tgaagacatg atgatctatc aattagaaga aatgaaagat gttaaaaaag atgtttccat
    42301 gcttaaagat actatgcgaa ttagcggaca acaagagttt gaaattaagc aaaaaggaaa
    42361 tatgaaagtt atggaagttc tagggggaaa agaaagccga gcttatgaag aaatcagcaa
    42421 aaaagtattc tcaaaatttt ggtctgaatt taaacgtacc ttttcaatcc caagatatgg
    42481 cgagttacct cgtaagagat tcgatgatgc tgtttcattt attgaaatgt ggttaccaga
    42541 aactgcgatc cgcatggaaa tcgatcaact gaacagacaa caaagacttt tcggtgatga
    42601 aaatgaatag agctgaagcg ctaagaatag ggacggtaat tgctaatcgc tggtggagac
    42661 acaataaacc aaacatccta agccaacaac atatcgaaaa gcaaaaagca tggcaacaaa
    42721 taaaaaagtg actcagccgg caagcataga gtcacaaaac atatctaagg agaattttag
    42781 catatgaata aagaactttc cactttagat caatatttga ctgattctga atggggcaag
    42841 tcgaatatca aggaaacaaa taatcgaaaa atcagacgaa atcttttgac gaacgaagaa
    42901 ctatcatgtg atcaagatga cttgggaaat tttgtgagta tttgggatca tgtttacctt
    42961 atccatctat caaaacattc aaataaacct gaatacatct atgtcatcga agatggcttg
    43021 attgatgcgc tagaagagta caacagagat aacttgattg atatctctta ttacggatca
    43081 ggtaagaaat acattgctga aatggaggca gaatttgatg agtgaaatca aagggacaac
    43141 gaactttgaa aaacttttta gtcgtaagtt aaataaaatt ctcaagaaaa aaggaaattt
    43201 tgattattta tcttgggctc acgcgtggga gattatgaaa aagaatgatc cacaggcaac
    43261 ggtaactatt aatgagtata aacactacag ggttgtttct ggaactcatc aagactttct
    43321 tgttgaggaa tataaacctt ttcttatgga tgaaacaggg acttatgtat ctgtctcagt
    43381 aacggttaaa ggacatacgg aaacaga
//
LOCUS       DAYYFU010000018        43368 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000018 DAYYFU010000000
VERSION     DAYYFU010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.018"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(8..2389)
                     /locus_tag="ACHA34_001170"
     CDS             complement(8..2389)
                     /locus_tag="ACHA34_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8791827.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2379..3026)
                     /gene="recU"
                     /locus_tag="ACHA34_001171"
     CDS             complement(2379..3026)
                     /gene="recU"
                     /locus_tag="ACHA34_001171"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFM8791828.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3090..3629
                     /locus_tag="ACHA34_001172"
     CDS             3090..3629
                     /locus_tag="ACHA34_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFM8791829.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3719..4138
                     /gene="gpsB"
                     /locus_tag="ACHA34_001173"
     CDS             3719..4138
                     /gene="gpsB"
                     /locus_tag="ACHA34_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFM8791830.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4160..4527
                     /gene="rnpB"
                     /locus_tag="ACHA34_001174"
     ncRNA           4160..4527
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHA34_001174"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4736..5902
                     /locus_tag="ACHA34_001175"
     CDS             4736..5902
                     /locus_tag="ACHA34_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFM8791831.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            5948..7444
                     /locus_tag="ACHA34_001176"
     CDS             5948..7444
                     /locus_tag="ACHA34_001176"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFM8791832.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7525..8208
                     /locus_tag="ACHA34_001177"
     CDS             7525..8208
                     /locus_tag="ACHA34_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8791833.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8853..10043
                     /locus_tag="ACHA34_001178"
     CDS             8853..10043
                     /locus_tag="ACHA34_001178"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFM8791834.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10145..11098
                     /locus_tag="ACHA34_001179"
     CDS             10145..11098
                     /locus_tag="ACHA34_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFM8791835.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11167..12516
                     /locus_tag="ACHA34_001180"
     CDS             11167..12516
                     /locus_tag="ACHA34_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8791836.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12699..13022)
                     /locus_tag="ACHA34_001181"
     CDS             complement(12699..13022)
                     /locus_tag="ACHA34_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791837.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13151..14314
                     /locus_tag="ACHA34_001182"
     CDS             13151..14314
                     /locus_tag="ACHA34_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8791838.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14384..15733
                     /locus_tag="ACHA34_001183"
     CDS             14384..15733
                     /locus_tag="ACHA34_001183"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8791839.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15778..16209)
                     /locus_tag="ACHA34_001184"
     CDS             complement(15778..16209)
                     /locus_tag="ACHA34_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8791840.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16597..19239
                     /gene="alaS"
                     /locus_tag="ACHA34_001185"
     CDS             16597..19239
                     /gene="alaS"
                     /locus_tag="ACHA34_001185"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFM8791841.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19439..20035
                     /locus_tag="ACHA34_001186"
     CDS             19439..20035
                     /locus_tag="ACHA34_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8791842.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20157..21305
                     /locus_tag="ACHA34_001187"
     CDS             20157..21305
                     /locus_tag="ACHA34_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8791843.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21343..21756)
                     /locus_tag="ACHA34_001188"
     CDS             complement(21343..21756)
                     /locus_tag="ACHA34_001188"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFM8791844.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            21835..22266
                     /locus_tag="ACHA34_001189"
     CDS             21835..22266
                     /locus_tag="ACHA34_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFM8791845.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22365..22598)
                     /locus_tag="ACHA34_001190"
     CDS             complement(22365..22598)
                     /locus_tag="ACHA34_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8791846.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            22781..24451
                     /locus_tag="ACHA34_001191"
     CDS             22781..24451
                     /locus_tag="ACHA34_001191"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFM8791847.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24598..24984
                     /locus_tag="ACHA34_001192"
     CDS             24598..24984
                     /locus_tag="ACHA34_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791848.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            24971..25669
                     /locus_tag="ACHA34_001193"
     CDS             24971..25669
                     /locus_tag="ACHA34_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFM8791849.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25674..26138
                     /gene="lspA"
                     /locus_tag="ACHA34_001194"
     CDS             25674..26138
                     /gene="lspA"
                     /locus_tag="ACHA34_001194"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFM8791850.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26135..27046
                     /locus_tag="ACHA34_001195"
     CDS             26135..27046
                     /locus_tag="ACHA34_001195"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8791851.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27281..27820
                     /gene="pyrR"
                     /locus_tag="ACHA34_001196"
     CDS             27281..27820
                     /gene="pyrR"
                     /locus_tag="ACHA34_001196"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFM8791852.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            27834..29117
                     /locus_tag="ACHA34_001197"
     CDS             27834..29117
                     /locus_tag="ACHA34_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFM8791853.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29130..30056
                     /locus_tag="ACHA34_001198"
     CDS             29130..30056
                     /locus_tag="ACHA34_001198"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFM8791854.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30075..31355
                     /locus_tag="ACHA34_001199"
     CDS             30075..31355
                     /locus_tag="ACHA34_001199"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFM8791855.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31360..32436
                     /locus_tag="ACHA34_001200"
     CDS             31360..32436
                     /locus_tag="ACHA34_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFM8791856.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32441..35626
                     /gene="carB"
                     /locus_tag="ACHA34_001201"
     CDS             32441..35626
                     /gene="carB"
                     /locus_tag="ACHA34_001201"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFM8791857.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35680..36456
                     /locus_tag="ACHA34_001202"
     CDS             35680..36456
                     /locus_tag="ACHA34_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFM8791858.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36453..37385
                     /locus_tag="ACHA34_001203"
     CDS             36453..37385
                     /locus_tag="ACHA34_001203"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFM8791859.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37382..38098
                     /gene="pyrF"
                     /locus_tag="ACHA34_001204"
     CDS             37382..38098
                     /gene="pyrF"
                     /locus_tag="ACHA34_001204"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFM8791860.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38105..38731
                     /gene="pyrE"
                     /locus_tag="ACHA34_001205"
                     /pseudo
     CDS             38105..38731
                     /gene="pyrE"
                     /locus_tag="ACHA34_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            38758..39036
                     /locus_tag="ACHA34_001206"
     CDS             38758..39036
                     /locus_tag="ACHA34_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791861.1"
                     /translation="MRFVSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFI
                     FLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            39074..39715
                     /locus_tag="ACHA34_001207"
     CDS             39074..39715
                     /locus_tag="ACHA34_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFM8791862.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            39758..40450
                     /locus_tag="ACHA34_001208"
     CDS             39758..40450
                     /locus_tag="ACHA34_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFM8791863.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40447..41349
                     /locus_tag="ACHA34_001209"
     CDS             40447..41349
                     /locus_tag="ACHA34_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8791864.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41507..43213)
                     /gene="efbA"
                     /locus_tag="ACHA34_001210"
     CDS             complement(41507..43213)
                     /gene="efbA"
                     /locus_tag="ACHA34_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFM8791865.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        13874 a         7663 c         9440 g        12391 t
ORIGIN      
        1 ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg tcatttgcag
       61 catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat gaagacggtg
      121 tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc tcgcttgttt
      181 ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta gtagagctag
      241 agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag ctggaagatg
      301 gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct ttgtagtaca
      361 gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt gtcacgttag
      421 ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac gattctgaag
      481 tgacaggtgt cattttttta ttgtaacctg tccacacgga aatcgaataa tttggcgtat
      541 agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt gcatactcgt
      601 catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg atttgcgcat
      661 tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata taagctgttt
      721 ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg tcttggaaga
      781 cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa gccgcatagg
      841 ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct tccgtattac
      901 tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt tttaagaaag
      961 aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca ttacgggata
     1021 agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag tttcctacag
     1081 gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca gtagaatatt
     1141 tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa ccaaaatcac
     1201 gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg tgacgtccgc
     1261 caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca acttgcatct
     1321 cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt ttcttttggg
     1381 cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc ttatctgttt
     1441 tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta gtattgtcat
     1501 cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct tgatcatatt
     1561 ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct cgtcttttct
     1621 tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc atcccagcaa
     1681 gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac atctcagaag
     1741 ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag taggtcaaga
     1801 tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct tgggccttgc
     1861 gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc aattgctgtg
     1921 tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca gaaccgatga
     1981 tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc gacacgatcg
     2041 catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt tctgcgccta
     2101 aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta gcgtctaatt
     2161 ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct acacctgcta
     2221 aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat aatccttttt
     2281 tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca ctctttttag
     2341 gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt cttgctttct
     2401 ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg aatccgcgga
     2461 gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg tagtgatttg
     2521 cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt caaataaaaa
     2581 cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg ttcgaggcat
     2641 gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga tgttttgttc
     2701 ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt atagtctgta
     2761 gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt aggatagtct
     2821 actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc gcgggaaaga
     2881 taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt gccgaattca
     2941 gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt ataataaggt
     3001 tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca ttatagcaaa
     3061 aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc tatgtgtctg
     3121 ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc caagttgtta
     3181 aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa tgggtacttg
     3241 tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa ttgaaagaag
     3301 aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt aataactgga
     3361 atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat gtcgaagctg
     3421 ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt tttatacttg
     3481 agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa acgaagtatt
     3541 tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg ctgatcagta
     3601 tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat caactttttt
     3661 cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag gtgtaaatat
     3721 ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa caaaaatgcg
     3781 cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag attatgaagc
     3841 ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg caaaagtagc
     3901 tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag aggcaccaaa
     3961 gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg aacgtgaagt
     4021 atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac ctaatccaaa
     4081 taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc aattttaatt
     4141 tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca ggctgaggaa
     4201 agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa accataagct
     4261 aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat gcttaagtat
     4321 ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga acgcggtaaa
     4381 cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa gaagaacagg
     4441 acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct ccctggaaaa
     4501 gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag gtcgtgacaa
     4561 aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa tacttcagat
     4621 gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat ttgagtaaaa
     4681 gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat tgtgtatgga
     4741 aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag cactggttgg
     4801 taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg cacgttttga
     4861 aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag atcgagtaaa
     4921 aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc
     4981 attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg gaaaatcgat
     5041 caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag caatcgttga
     5101 acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa ctggcgcaca
     5161 ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg atacgacagg
     5221 accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa
     5281 catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc
     5341 tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata atatcgcacc
     5401 aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga
     5461 aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag atattactgg
     5521 ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag aaatcggatt
     5581 aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg ataaagaata
     5641 cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag aaactgttcg
     5701 gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga gtaaatatat
     5761 cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa
     5821 attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc gtccgcctag
     5881 aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat gtttggagga
     5941 atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc ttctacaaca
     6001 agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag ctagtgcata
     6061 ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa tcggtccaaa
     6121 aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg aagttggaac
     6181 tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac cggaagatct
     6241 tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa aagctagaga
     6301 aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat taacaaaaca
     6361 gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc tgaatcaata
     6421 cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta aagaaggaat
     6481 tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag actttttaaa
     6541 tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg aacaacttgg
     6601 ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga ctggaattaa
     6661 tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt
     6721 tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg ataaatatgc
     6781 gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat cgttgtttaa
     6841 cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt tctttcaaga
     6901 atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt cattgaacca
     6961 tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc tccatatcat
     7021 cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg ctgaactacc
     7081 tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa atgatgtgga
     7141 aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc cctcttatgc
     7201 attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa tagatgtgga
     7261 aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag agcatatcca
     7321 tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac atgaaccgct
     7381 taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat atcaggtaaa
     7441 ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt tttcagctat
     7501 ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga ttatagtatg
     7561 gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag agttgtcaag
     7621 ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac gcttgcagca
     7681 gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa
     7741 cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc gatgattttt
     7801 tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga actatctctg
     7861 gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat cggcattttg
     7921 cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga actgtatccg
     7981 caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa tcatttttat
     8041 gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga agaagccaaa
     8101 cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg gtctattgac
     8161 gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg gaggttgtga
     8221 cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac ttctggaaca
     8281 ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga acaatttcaa
     8341 aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg gcaggagaac
     8401 accgtttatt cggaagaata ggactgtgac aagacttttg tcacagtcct tttttcgaat
     8461 tcaacaagta aaacttttaa gaaatattca gagagtaaaa ttaaaaattg ctgataaacg
     8521 aagatggaaa ttttatgtat ttttattggt ctatgcaata aataaattaa atattcagaa
     8581 tacaattgat tttaacgaaa gggcttctat caatggttgt gttgtattta tctgcccagg
     8641 tagagtaaaa tagatcatta tctaaaaggt aattgttcat actatataag tcatttgaaa
     8701 gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt ctcataatca gtgtaagcgt
     8761 tatcaaaaat atagcatact tgctttgtag atgaatgaca cgatatatgt ttggtatgtt
     8821 ctgcaagaca gatgaatatg gaggtggaga caatgtcttc caaagaacta acaatgtatt
     8881 tagagaaaga attggatcaa ctgaaagaaa aggggttata caatacgatc gacatactag
     8941 aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa gatgatcaat ctagcatcca
     9001 acaattatct tggatttgcc aatcgcgaag aattgaaaaa agcatgtatt gaagcaaccg
     9061 aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa tggttcgctg aaaatccatc
     9121 agcaattaga agaaaaaatc gcagaattca aagggacaga agcagcaatc gcctttcaat
     9181 ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat gacaaaagaa gatgctatct
     9241 tatcggatga attaaaccat gcatctatta ttgacggttg ccgcttatcc ggagcaaaaa
     9301 tcatccgaat taaacatcaa gatatgaaag accttgagaa aaaagcaaag gaagcgatag
     9361 aaagtaagaa atataaaaaa atcatgtata tcacagatgg tgttttctcc atggatggag
     9421 acattgcacg attgccggaa attattccga ttgttgaaaa atatggactg atcacctacg
     9481 tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc aggaaccgtc aagcattttg
     9541 gattaagcga caaaatcgat atgcaaatgg gcacgttatc gaaagctata ggtgttgttg
     9601 ggggttatgt agctggatcg aaaacactaa tcgactggtt gaaagctaga agtcggccat
     9661 tcctattttc tacttcgcta actccaggtg cagctgcagc tgcattggct tcgattactt
     9721 tgatgcaaga acatccggaa cttgtcgaaa aagtttggga aaatgctaat tattttaaag
     9781 aagaattgaa aaaagtaggc tataacatcg gtatgtctga aacacctatt actccagtca
     9841 ttttgggaga cgaaaaagtg actcagactt tctctaagaa gctgatcgaa catgggattt
     9901 atgctaaacc aatcgtttat ccaactgtgc ctcttgggac aggcaggatc agaaatatgc
     9961 cgactgcgga acatacgagg gaaatgcttg atgaagcagt agcagtttat caaaaaatag
    10021 gaaaagaaat ggaaattatt taactaaatt tagttttttc gtctatttaa aattagagaa
    10081 catgttcacg catatattgt taatcatctg aagatagtag taaaaaagat cagggaggat
    10141 catcatgaaa aaagtattgg tgacaggatg tttaggacaa attggttcag agttgatcac
    10201 acggctacgt caagaactag gcagtgaaaa tgtcattgcg acagatatac gcatgcctga
    10261 agtcattgag aaaaacgata tctttgagat cctagatgta atggattacg aaaaaatgcg
    10321 cagcatagca gaacaatatc aagtggacac attgattcat ttggctgctc ttttgtcagc
    10381 tgttgcagaa gcaaaaccac aattagcctg gaatttgaac gtgactgggc tggtcaatgc
    10441 attggaagtc gctagagaat tccagctgaa attttttacg ccaagttcaa tcggcgcgtt
    10501 tggtccaaat actccaaaag atcatacccc acaagatact cttcagcgtc ctacaaccat
    10561 gtatggggta acgaaggtag ctggagaact actatgtgat tattattatg tgaaatatgg
    10621 cgtagatact cgtggtgtcc gtttccctgg attgatttct tatgcaacct tgccaggagg
    10681 cggtacgaca gactacgcag tcgatattta ttattcagca atcaaagaag gaaagtacac
    10741 tagtttcatt gatcaaggta catcaatgga tatgatgtat atgccagatg cgatcgatgc
    10801 cattataaaa ctcatgaatg cagatcctga tcgattggtt catcgaaatg ccttcaatat
    10861 atcggcgatg tcttttgacc cggaagagat caaagcatct attcaaaaaa tcataccaga
    10921 ttttgaaatg gattatgatg tggatcctgt acgtcagtca atagcagaat cttggccaaa
    10981 ttcactagat gtttcttgtg cgcaagagga atggggcttt tcgccgaaat acgacttaga
    11041 tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa ttggtagaga cggtataaaa
    11101 caagaaccac atccaaaaag ttttttatac ttttgggatt ggctatggta tgaaagggga
    11161 caagggatgg aagaatatca agccacgcca gtaaaagaag taaaaataga aaatgaatta
    11221 aaacgtacga tgggattttt tactgcttta tcgactgtta tgggcacagt gatcggtgca
    11281 ggggtatttt ttaaagccgc aagcgtagca gaagttacag gatccgccag tttgcatatg
    11341 ttttcttggt ttctcggtgg tatgatttct gtctgtgcag gactaacagg agctgaactg
    11401 gcagctgcta ttccagaaac aggagggatg attaaatata tcgaacggat ttacggaaat
    11461 acagcagctt ttttattggg ctgggcgcaa gtcgtgattt attttccagc aaatgtagca
    11521 gccttgtcga tcattttcgg tacacagttc gtcaatctat ttggtctttc ccagtcaatg
    11581 attgttcccg tagctgttac agctgctgta tcgatcctgt tgatcaattt tttaggttct
    11641 aaagctggag gggcttttca gtcgattacg cttgtctgca agcttattcc tttgtttgtg
    11701 atcgtcattt tcggtttgtt ccgacaagaa ggagtagact tccaactgtt tccgatccaa
    11761 gcaggagaaa atctttcatt tttctcagcc ttgggagcgg gcttgctagc aacgatgttc
    11821 gcatacgacg gttggataca cgtaggaaat atttcgggag aattgaaaaa gccggcgaaa
    11881 gatttgccaa aagcaatttc tttaggaatc atcggaatca tgatcgttta tttattagta
    11941 aatgcggtgt tcttaagaac cgcatctatc gacggagtcg caggaaacag caatgcggca
    12001 agtgacgttg caaaaatgat ttttggcggc ttcggtggaa gactggtcac agttggtatc
    12061 ttgatttctg tttatggaac catcaatggc tataccctta ctggaatgcg tctcccttat
    12121 gttatggcaa aagaaaacaa cctgcctttt agcaagctct ttgctaaact tcatgataaa
    12181 actaaagttc ctgtagccgc aggtatctta gagctagtca tcgctattgg catgatgatg
    12241 atcggtggtt ttgatacact aacggatatg ctgatctttg tcatttggat tttttatacg
    12301 atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac ctgatttgtt tcgaccatac
    12361 aaagttccaa tgtatccgtt tattccacta gtagcaatca ttggagggac ttttatcgta
    12421 tcaagcacgc ttatcactca gaccttttta gcttcgatgg ggatagtatt gacacttgct
    12481 ggtatcccga tttatctcta tttaaaacgt agatagctaa aacattcgaa ttttattaga
    12541 actgcacttc tctgccaaac aaaatatttt atttaaatga ggagtacgtg gaagaaatgt
    12601 caaaagtact cgttaatttt gatcacgtaa atgtcgtttt gtctacgatg aaatagcaaa
    12661 gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt actttgattt gcgtaactga
    12721 actattcggc gtacgccgcg ataaatattt aataaaacca agcaaccaga aatgaaaatg
    12781 ggaagccaaa tcagatagac agggtagcca agcactttag acaaagcatg tggagtcatt
    12841 ttatccattt caagaagtgc ttgctgccaa gtctgaagat tgaagactag catgaaaccc
    12901 gaaataatca ggagaataaa tcctaaataa tgaattgtag ttctttttct tacataagcg
    12961 atcgtagcat aaaggaacag taaaaaaata ggtacgccaa aaacaaagta catattcagc
    13021 atctcgacac cccctttgtg tttatcttac tttactttga aagaaatgag aaataatagc
    13081 ctcgttactt gcttgaaaca gcaaaggact gctaagataa gagagataca aagaatcaag
    13141 gaggagtcat atggcaagtt atatcgaacc gattcgtttt gcgctgattg tttttccatt
    13201 tttagcctta gcgatctcat ctgttttttt tatttatgaa tatcgaaagt atggaacatt
    13261 tctagtaaca agggcagtta ttttgtattc tttcgttttt tatttattgt gtgcgtattt
    13321 tttggtgatc ttaccacttc cggcaaagga gacggttgcc caaatgacag ggccaaagat
    13381 ggagctgcat ttatttgctt catggcaaca tttcagagac aacacggtat tggtcttgtc
    13441 tgaacctagt acctacctgc cggcaatgaa gcagagtgta tttttggaac cagcttttaa
    13501 tgttttatta ctcataccat ttggcattta tttaagatat tatttccggc tttcttttat
    13561 gaaaacgatg attgctagtt tctgcctttc tctatttttt gaattgacgc agctaagtgg
    13621 actttatttt atctacccaa gaccataccg cttatttgat gtgaatgatt tagtgacaaa
    13681 tacattgggt ggaatcattg gttttgtcgt tacgccgtta tttacattta tgcttcctac
    13741 aagaagacga atggatgaac tttcttatga aaaaggaaag actgtctcat tgactcgacg
    13801 cttggtcgct tatttgatcg actggacatt tttatctctt attaatttgg tagcggcgat
    13861 tttctttaaa attgcgttcg attcaggggc agttacttct catatctggt ggcttttatt
    13921 agaagtaggg gtatacttta tgattgtacc gtttataacc aatggatgga cacttggcaa
    13981 aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa cgaatttctt taaaagcatt
    14041 gatgatccgc tacgggtatc tgtactttct tttttatggt ttaagctggt tggggcagtt
    14101 gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg cagatgagtg cagtattcat
    14161 ttctctcggt gtcttgttac tacaaggtat ttttatttta aatatcttgc tatcattttt
    14221 gagaaaaaaa cggattctgt tttatgaaaa agctagtcac acatatactg ttagcacgat
    14281 tggccaaaca aaggatgatg attctcgaaa ttagctgagc aaaaacgctt tgtctgtgtt
    14341 ataatgccaa agtcgtgata atttaaagag aagaggtgca cgaatgaata attttgatat
    14401 attccgcttc aaaccgttta tcaatgaagc gttaaccgca aagggcttca aacagcccac
    14461 agaggtccag gaacgtttga ttcctttaat tgcaaaagga aaaaatatca ttgggcaatc
    14521 tcaaactggt acaggaaaaa cacatacgtt tttgttgcct ttgtttgaaa aaatcaatgc
    14581 agaaaaagag gaagtacaag ttgttattac agctccaagt agggaactag ctgcacagat
    14641 ctatgaagca gctacacaaa tcgctaaatt ttctacaccg gagatccgtg tctcgaattt
    14701 tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg catcaccaac agccacaggt
    14761 tgtagtaggc acgcctggta gaatattaga catgatgaat gaacaagcgc tgaagattca
    14821 caccgcattt gcatttgttg tcgatgaagc ggatatgaca ttggatatgg gttttttaaa
    14881 tgaagtagac caaattgcaa gtcgattacc agaaaagctg cagtttttag ttttttctgc
    14941 cactattcca gaaaaacttc gtccattctt acgaaaatac atggaaaatc caatcattga
    15001 agaaatcaaa ccaaaagcag ttatttctga agacattgaa aattggctga tttcaacaaa
    15061 aggtaaagat gagaatcaaa tgatttatca actgctgaca attggtcatc cttatttagc
    15121 aatcgtattt gccaatacaa aacagcatgt ggatgaaatt gccgattact tgaaaaacca
    15181 aggcttgaaa gtagcaaaga ttcatggaga catcacccca agagagagaa aacgagtgat
    15241 gcgacaagta cagaatctgg agtatcaata tgttgtagcg actgatttag ctgctagagg
    15301 gatcgatatc gaaggtgtct ctcatgttat taatgcagaa attccagaag atctagactt
    15361 ctttatccat cgtgtcggac gtactggacg taatggctta aaaggaacgg ccattacgtt
    15421 atattcgcca aatgatgagc aagccattga tgcgatagaa aaattgggcg tctcatttga
    15481 accaaaagaa atcaaaaacg gggaaatcgt caaaacatac gatcgtaata gacgtgccaa
    15541 acgagaaaaa tcaaaagatg tattagatcc cacgctgatt ggtcttgtga agaagaaaaa
    15601 gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg gcaattgcag aaaaaaataa
    15661 aaaagaacgc aaaatcgaac gcagacagca aagccgtact gctcgtaaaa ataagaaaaa
    15721 ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt gtcacaatct cttgttttta
    15781 agctagactt tttttcattc cgatacaaag tacttcatct tcaataaaag gttctgtgca
    15841 agtatgatac ccttgttttt cataaaaatc tacagccgtc atttcagcta ctacataact
    15901 agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct tcaagcaact gaccgcctaa
    15961 cccttttcct cggtattctt ttgctacgca aaatcgttcc ggcttgaagc aagtaggtgt
    16021 tttcttttca taacgcaaag tagctactgg tcttttttga tcaagaaata gataataggt
    16081 ttgttgcggg atatcttttc cgtcgaattc tagttctgca ggaatccctt gttctaatac
    16141 aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc cattcatccg ctccaactat
    16201 ttttttcatc atattcctcc ttgtcatgaa tagcagagat gtattgattc ctttattctt
    16261 atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga aaataaaaat tgagatattg
    16321 acagtgatca atagaatgcg tatactaatt aagtacgaaa aggatatgat ttgtttttta
    16381 agactcacag aaaattcttt cttggctgaa aaaagaatag tcactaatca aattgctccc
    16441 tttttctatt ttggtagaaa taccgacgca gctcagcgtt atcgagttta agaggtaatg
    16501 attacacatc attgcaatca aggtggtacc gcgagttttc gtccttggct gtgatggtgt
    16561 ttttttgtat ttaatttata gaaagcaggt aatgacatga aacaattaag tagtgcacaa
    16621 gttcgacaaa tgtttcttga tttcttccaa tctaaaggac atacgattga acctagtgcg
    16681 tctttgatcc ccgtgaacga tccaacctta ttgtggatca actctggtgt tgcaacattg
    16741 aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa gaatcacaaa tgcccaaaaa
    16801 tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag cacggcacca tacgatgttt
    16861 gagatgctgg gtaatttttc gattggtgac tatttcaaaa atgaagccat ccactgggca
    16921 tgggaatttt tgactgatcc aaaatggatg gcattcgatc cagaaaaact ttatgtaaca
    16981 gtttatccaa aagatacaga agcaaaacgc atctggcatg aagaagttgg cctgccacta
    17041 gatcacatta ttgatgtaga agacaacttc tgggatatcg gtgcaggccc atgtggtccg
    17101 gatacggaaa ttttctatga ccgtggagaa gcttataacg atttagcaga agacgatcct
    17161 gaaaactatc caggaggaga aaatgaacgt tatttagaaa tctggaactt agtattttca
    17221 gaattcaacc ataagcctga taacacattt gaaccacttc ctcataaaaa cattgatacg
    17281 gggatgggat tggaacgcat ggtctcaatc gttcaagatg cgtcaacgaa ctttgagaca
    17341 gacttgttta tgcctattat ccacgccgta gaaaagctaa gcaaacaagt aaaatatggt
    17401 gaaaacccag taacagatat ttcttacaaa gtaatcgcag atcatattcg cgccctttca
    17461 tttgctattg gcgacggtgc attgccatcg aatgaaggga gagggtatgt cttacgccgt
    17521 ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta ttgaacaagc attcttatat
    17581 cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt atcctgaagt acttcaacaa
    17641 aaagaattta ttgaaaaagt tatccgtaca gaagaagaac gtttcaatga aacaatcaat
    17701 gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa aaaacgaaaa cgggacgact
    17761 ctagcaggaa aagacatctt caaactatac gacacatatg gcttcccggt agaattaacg
    17821 gaagaggtgg cagaagatga aggactgaaa gttgatcatg ctggttttga aaaagaaatg
    17881 gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg aaaaatcaat gggggtacaa
    17941 tctgctttat tgacagatat caaagtagaa agtaaattca ttggatacac agaacaagaa
    18001 tcagatagtc aattattggt catcgttaaa ggtgagtctc ttttgaatga agtcgcagaa
    18061 ggtgaagctc aaatgatctt tgatcaaaca cctttctatg cagaaatggg tgggcaagta
    18121 gcggatcaag gatggatcat gaatgaagta ggcgaaatcg tagcagttgt tacagatgtt
    18181 caaaaagcgc ctaatggtca atttatgcac accgtagaag taaaagcacc aatgagtgaa
    18241 ggacaaacct atcatctatt gatcgatcaa tcgatgcgca atcggatcat caaaaaccat
    18301 acagcaaccc atctactgca caaggcattg aaagaagtac ttggcgagca tgcaaatcaa
    18361 gctggttctt tagtcgctcc tggacattta cgattcgact ttacccactt tggtcaaatc
    18421 acttcagatg aattagctga aatggaaaga atcgtcaacg aaaaaatctg ggaagctatc
    18481 ccagttgaaa caattgaaac agatatcgat acagccaaaa acatgggtgc aatggctttg
    18541 tttggtgaaa aatacggaca tgacgttcga gtagtaaata ttggcggctg gtcaatcgaa
    18601 ctgtgcggag gcacacatgt agcaaataca gaaaacatcg gaatcttcaa aatcgtttct
    18661 gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg taacaagtaa agaagcgtac
    18721 gaactacttg aagaagaaga aaaacaacta aaacaagtag caacaattgt taaatcacct
    18781 caattgaaag aagttgtttc aaaagcggaa caattgcaac agcaagtaag agagctacaa
    18841 aaggaaaatg aacaattagc tagcaagtta gcgaatcaac aagctggtga tgtattcaaa
    18901 gatccagaaa cgatcaatgg aaaaacaatc attgcagcac aagtaaaagt gaaagatatg
    18961 aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa atatttcaga tattcttgtt
    19021 ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg caatgacgaa agaatcaaat
    19081 gaagctggac taaaagctgg cgacttgatt aaagcaatcg caccgaaagt aggcggtggc
    19141 ggtggtggac gtccagacat ggcacaagct ggcgggaaaa atcctgctgg tattccagat
    19201 gcacttaaag ctgcgaaaga atgggttgcg caacaatagg gcgtttacgt ttgacagaaa
    19261 aacaaaagtt caaacattga acgaaacatg attatggggg cgaagacaaa caagtcttgc
    19321 cctcatactt ttttcaattg gatggcgcat ctaatcaaat ggtcggattc ggatggtctt
    19381 tagttgacga aagtgacaga aatttttacg attagtataa ataaaaaggg gtgaaaaaat
    19441 gaatagtaga aataagacct ttcgactagt cttacgtgcg atcttgttag caatcatcat
    19501 cgtacaagcg atggttcctt ggctggggtt tattccatta ggattcatta gtttaacaat
    19561 tatccatatc acagtaatta ttgctgcagt agtattaggc ccaaaagatg gcatggtgat
    19621 tggcttgttt tggggaatcg ctacaattgt tcgtgcctat gccatgccaa caacgccatt
    19681 tgatacatta gtgttcacga atccgattat ctcggtagtt cccagagtac ttgttggctt
    19741 agtagcaggc ttggttttcc actggattta tcaaaaaaat aaatcaataa cgatcagctc
    19801 agcctttgca ggggtactgg gttcattggt caatacagtc ttagtgttag gattcatggg
    19861 attattttat acaggtgcaa cggcagatgc ctatggtgta gactctgctc ttttatttaa
    19921 aaccttagca ggggttgcag caatcaatgg gataccagaa gcaataggtg cgggaatcat
    19981 aacaccatta ctagcaaaag cgttatttgc agcaacaccg ttgaaaccag aataatgacc
    20041 atcttttaat agtaatagga cgcacgatag atagaaaaag gaagagagga gaggaaaaca
    20101 atcgtcctct ccatcttttt acctgagcaa caagcaaact agaaggggta aaaaacttgg
    20161 ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca gcggacaaaa aaaggagtac
    20221 tagcgggtat cttaggattg gttacgaatc tgatcttgtt tgtctctaag ctaatgatcg
    20281 ggcttttttc aggaagtgtc tccattatgg ctgatgcagt gaacagtcta tcagatactg
    20341 cttcatccat attgactttg atcggattta aaattgctgc aaagccagca gatcaggaac
    20401 atccttatgg acacgaacgc ttcgaatata tcagtgggtt atttgtttcg atcatcatta
    20461 cttatgtagg atttcaattt ttagagtctt ctgctgaaaa aatcatccat ccagaaaatg
    20521 tctctttaac acccatcgtc ttttttgtcc taatcatttc catctttttg aaatttgcac
    20581 aaggcaaaat gtatcaggtt attgcgcaat ccattgattc tgaaacattg agagctacag
    20641 caacagatag ttataatgat gtctttacta cactggctgt attgctttca gcagcaatcg
    20701 aatgggtaac tggatggcgt attgatggat atataggttt tgctttagca ttatatatcc
    20761 tttatagtgg attgacgatg cttcgaaatt ttgtatacga actattagga agtcgcccaa
    20821 cgccagcaga gattgaagaa atggaagagc atttaaatgc ttgcgatgat atacttggct
    20881 atcatgattt attggtgcat aactatggac ccaataaacg ctttgcatca gtccacatcg
    20941 aagtagatga atcaatgaat ttgaatgtgg ctcataagat tattgatctg atagaaaaag
    21001 actttaaaaa gtctttggat gtggaacttg tctgtcactt ggatccaatg cctgttgata
    21061 acgaaaaata ctataatatc ttgcggcagt taaagcaaat tcttcttgaa acaggtacag
    21121 ggcttaatat tcatgatttt agagtaattc atcaaggaaa aacgctgcaa tttgatgtag
    21181 tggtccctga gaaattccag tttgttgata cagaactaga aagaatctta cgtagtcaaa
    21241 tagaagaaaa aatcggagat taccaattgg aaattacgtt tgatcacaat tatcttttat
    21301 attaaatgag aaactataaa aagactcctt aaagttttta atttaaagag tctttttttc
    21361 ttttaattgt ttatggagag cctgacgtgc aagattatct gctcctttat ttttgttttc
    21421 gggcaaccat tgcaggatca ataaagaaaa ttcggataga agttcttgga ttctttgaag
    21481 gtagggaagg aagagctcgt tcttcgttgc gtttttgtcg accgtagaaa ctagtgtttt
    21541 gctgtctgaa aaacagaaaa tcgtttcatt ctgtaagtcg tttttcttta gaaattctaa
    21601 agtttttaaa aagacttcga actctgcttg atggtttgtc cctatagaaa gcgggataga
    21661 tagctgttct tgtttacctt gatagacaat caagattcca cctccactag gaccagggtt
    21721 gccttttgta gaggcatcaa tgtatacttt taacataaaa agagattctc ctttttgttc
    21781 aaactaatgt tatggtaaga ttatatcatt actgaatcgg tgagaggtta gagaatgaaa
    21841 aaaaatattc gatggcagcc tgaactggca catacgatca tctattggtc attgaccttg
    21901 atcattctgt tttttagtct gattctttct ttagaaaaca caggtcctta ttggaaaagc
    21961 aattttgttt taggtatttt tcttgttttt ctatttctag gtttgcggcg ttcttttatt
    22021 ttaaaatcaa actctgtgat tatacgttat gctgcttttt ggaaagaccg agaaatatct
    22081 gtcggacaga ttcgatcttt gacgccaact aatactgggg tgcaaatgat gttgcaagat
    22141 gagaaagccc catctgtttt catgatgcga aaaaaagatc aagtagtcct ttttgatcat
    22201 tttgaaaaaa acggacccaa atcagaatca attccttatc cttatacgaa aaaaagaaaa
    22261 agctaatttt aaaaatcaga acctaaaaaa acgaattttt acaggaaaag aaaagagtga
    22321 gacgtacggt tcatcccgtt tagcgtctca ctctatttgt ttatctattc atgcatacta
    22381 gtatcggtag tctgaacgtt gcctttcttg acatgggcag cttgtgttcc tcgattccct
    22441 tcgatgatct caaattcgac cacttgattt tcttccaaag ttttgaatcc ctcttcttca
    22501 atcgcagtaa aatgtacaaa aatttcttct gtgtcatcat aactgataaa gccgtatcct
    22561 tttttgttgt caaaccattt tacaattcct gtggtcaaag cactcacgac ctttctaaag
    22621 tctgatcata gccttcatta taaagagtat ttataagtac gtcaaataaa ttaaaaacct
    22681 gtaattttat gttttttttc tgaataaaaa aaggtataat tggcatatct taacaaagaa
    22741 gatattttgt tgaataaagt tcgtgaaaag gggtaatcaa atgaagaatg atctgcaaat
    22801 ttcacaagaa acagagctag aaccgattgt tgaagtggct agtaaattag atcttaagga
    22861 agacgatttg gaactgtatg ggaaatataa ggcgaaaatc aatttttctg gaatcaatcg
    22921 atcaaaggat aacgctgagg ggcatttgat cttagttact tccatcaatc caacaccagc
    22981 tggcgaagga aagtcaacga tcactgtagg gttaggagac gcgctcaata aaatcaataa
    23041 aaaatctgtt attgcattgc gagaaccctc acttggtcct gtgatgggaa tcaaaggtgg
    23101 tgctgcaggc ggaggatatg cacaggtatt gcctatggaa gatatcaact tgcattttac
    23161 tggtgacatg catgctatca caacagctaa taatgcatta tctgctcttt tagataatca
    23221 tattcatcaa ggaaatgaat tgaacatcga tgcccgccga gtgatttgga aacgggtagt
    23281 cgatctcaat gatcgtgaac tgcgtaaagt aattgttggc ttgggtgggc caatccaagg
    23341 tgttccaaga gaagatgggt ttgatatcac agttgccagc gaaatcatgg ctatcttgtg
    23401 tcttgcggca gaccttgaag atttaaaggc tcgtcttgca aggatcgttg tcggctatac
    23461 atttgaccgt cagcctgtga ctgctggaga cttgaaagca gaaggcgctc tggcattact
    23521 gctgaaagat gcaatcaagc cgaatcttgt acaaacaatt tacggaacac cagcttttgt
    23581 ccacggggga ccgtttgcca atatcgctca tggctgcaat agtattttag ctaccaagac
    23641 agcattgaga ttagcagatt acgttgtcac agaggcagga tttggtgcag atcttggcgg
    23701 agagaaattt ttagatatca aagtacctaa cttgaaaaaa gcgccggatg ctgtggtgat
    23761 tgtcgccact gtccgtgcat tgaaaatgca tggcggaatg aaaaaagatg aacttaaaaa
    23821 tgagaatctt gatgctttga aagtaggttt tgcaaattta aagcgccata tacgtaatat
    23881 ggaacaatat cagctacctg tgatcgtcgc aatcaatgaa ttcgtaacag atacagacaa
    23941 tgaactcaca ctgttggagc acttgtgtga aaatcagggg attcttgcca agagagcgag
    24001 tgtatgggca gatggagcag aaggcggcgt tgatttagca aaagctgtag tacgattgat
    24061 tgatagaaaa gaagctaatt acaagccatt ataccgccta gaagaaacga tacaagaaaa
    24121 gacagaaaca atcgtcaaga aaatttacgg aggaaatggt gttgttttca gcaaaaaagc
    24181 gcaaaaacaa atagaagagt ttacgaagaa tggatgggac aaactgccga tttgtatggc
    24241 aaaaacacag tattcatttt ctgatgatcc ttcactgcta ggtgcaccgg aagatttcac
    24301 catcacgatc cgcgaaatca tcccaaaatt aggggcagga tttttagtag ccttgactgg
    24361 agatgttatg acgatgcctg gtttgcctaa aaaacctgct gcattgaata tggacgtgac
    24421 aaatgacgga gaagtgttgg gattgtttta agcttgatta taatctacca tcgaaggagc
    24481 tgtattttga ctacagcttc tttttttaga cgatagggaa ttaattttgt ttacactagg
    24541 atcattcttt ttctttattg tattttgagc tatactaaag cataatagga gggtatgatg
    24601 aaaacgacat ttataagaaa aaccggtttt tacggtatgg gtagctcgat tcagctaaga
    24661 atcaacaatg aaaaacgcag tgttctcagt catgaacaaa agataacgct tgatctggaa
    24721 gtgcccttta cgatgcaggt caccttttat tggctaaaaa gccccgttta tacagtgaaa
    24781 gaagcaaagc ctgcttatgt gatcacaatg aattacttga ttcttcaaat ctatccgttt
    24841 ttatttctag caactgggtt gggggccgtt ttcctacaaa atttattttt cagttttttt
    24901 gcagttattg tgttgattgc gttctttttc tatataaaaa accgggcata tttgatcaag
    24961 gaggaaaatt atggcaaact ctgatctttt tttaatcagt ttcaatcgaa tcgaaaaatg
    25021 gatgagagac gagatgggaa atgcacgaaa tatgggattt accgaacttg tcagacgcct
    25081 agctcaaaga aaacaattaa tgatcagaaa atacgaagat gatcttttac agcttgctca
    25141 gttaaggaat gcgatcgtcc atgatcggat cgcagtcgat tttgtcattg cagagccaaa
    25201 tgattggaca gtcaagcgaa tccagctgat cgaacaagaa ctgattcagc ctgaaaccgt
    25261 gctgcctcga tttgcaaaac gtgtaacagg ttttgaacaa gatcttccgt tacttgaact
    25321 gttaaagata gtagcagaaa aaaggtattc acaatttccg ttatacaata aaggaaaatt
    25381 tgtagcattg attactttac gtaatatcgg gttttggcta gcaaaagaaa gccaaaaagg
    25441 tccggttgat ttaacaaata aacgagcgtt ggatttgatc atccaaaacg gaaagtacac
    25501 aaattatcat tttgttccag ctaaaacaca tatttacgag gttgaagcga tgtttcgtga
    25561 gcaagggact ttggaagccg tacttatcac taaagatggg aatcccgatg gtaatctgct
    25621 cgggatcgta cgacctcgtg atatttataa acaaatagaa aaggattgat tttttgttaa
    25681 ttatttattg gatattcagc gctttgctag taggagtgga tcagttggtc aaatggtgga
    25741 tcactgataa tctagcgctt ggagagacga aaaatcttat accaggtatt ttgtcactga
    25801 atcatattcg aaacactgga gccgcatgga gtatgctaga agggaagatg tggttcttta
    25861 ctatagtgac actcattgca gtcgttgtga ttctcacatt gatgattaaa aatcgtgaaa
    25921 aaggtaatcg ctggttcatg attggattga gtttgattct agctggtgcg attggcaatt
    25981 ttatcgaccg tattcgtctt ggctatgtag tagatatgtt ccagacagat tttatgagct
    26041 ttccgatttt taacgtagca gatgtgacac ttgtatgtgg agtgatttgt atgttaattt
    26101 atattatttt agatgaaaag gatcaaagaa aaaaatgaca gaacttaaaa cgacgattca
    26161 ccatgaatct ggtaggatcg ataaagtatt gactaattta ttcaccgatt acagtcgttc
    26221 gcaaattcag ctgtggctga aaaatggagc ggtttctgtc aatggacagc cagtcaaagc
    26281 aaattacaaa gtaaaacaag gagacgagat tactgtcttg atccccgaac cagaaacatt
    26341 agatgtggtc ccagaaaata tcccgttgac gatcgtttac gaagatgaag atgtagcggt
    26401 gataaacaaa cctcaaggaa tggtcgttca tccttcagca ggtcatacgc atggcacaat
    26461 ggtcaatgcg ctgatgtacc atatcaagga tctttctacg atcaacggcg ttattcgtcc
    26521 aggtattgtt caccgaatcg acaaagatac ttccggactg ctaatggttg ccaaaaatga
    26581 ccaggcacac gaatcattag ctcagcaatt aaaagataag acctctttgc gcaaatatgt
    26641 tgctttagtc catggagaga ttcctcacga aaaaggaacc atcaatgcgc caatcggccg
    26701 ttcgaaggaa aaccggaaaa tgcaggcgat aagagaagat gggaagtcgg ctgtcacaca
    26761 ttttactgta ttggaacgtt tcgaaggatt tactctggtt gaattaaaac tagaaaccgg
    26821 aagaacacac cagatccgtg tgcatatgaa gtatatcggt tatccgttag ctggagatcc
    26881 agtatacgga ccgaagaaaa ctttgaaagg caatggtcag tttctccatg cgcaaatgtt
    26941 aggttttacg cacccgacta caaacgaaaa gatgatcttt gaagcacctc ttccagaagt
    27001 ttttgaaaaa actttagaaa aattaagaaa aaacattgca ttttaattta gataacggta
    27061 tagtaattaa caacgaaagc ataaaactta atagaattcc tttaagttta gtcccgtgag
    27121 gctaagaagg gtgggagcaa ggatacgtat ggtgcgccat aagtagattg ctcattgaca
    27181 tagtatagct atcagtgtat cagtatacgc tcagtctaaa cattcctcct gtcctcttag
    27241 ggcaggagtt tttttatgca aaaaagtagg gggaatgaaa atgcaagcaa aagaagtagt
    27301 agatcaggta accatgaagc gagcattgac tcgaattact tatgagatta tcgagagaaa
    27361 ccatagtatc caagatatcg tattggtagg tatcaaaaca cgaggaatct acattgcatc
    27421 aagaattgcc gaacgcctga agcagctaga agatatcgat attcctgtag gagaactgga
    27481 tatcacttta taccgagatg acaaaaaaga aaatccagaa gaaccggaac ttcattcttc
    27541 tgacatccca gtttccttag aaggaaaaga agttattttg attgatgatg ttttgtatac
    27601 aggacgaacg attcgagcag caatggacgc agtaatggat tttggcagac caagaaaaat
    27661 ttcattagca gtcctggtcg atcgtgggca tcgagaattg ccaatccgtg cagattatgt
    27721 tgggaagaac attccaacat ctcgggcgga agagatttta gtagagatgc aagagcttga
    27781 tggacaagat cgcatcatga ttttgaaaga ggaagattag aggagagaga aacatgtcgg
    27841 agaaaaaagg atttcataat gatgacgtgg tgttagatat ccacgatcgc ccaaccccag
    27901 gacattgggt aggattaagt ttacaacact tgttcacgat gttcggagca accgtgttag
    27961 tccccatctt agtaggtatc gatccaggta ttgcgctagt cagttcagga ctaggtacat
    28021 tggtgtactt atttactact aaaggaaaga tacctgcgta cttaggaagc agcttcgctt
    28081 tcattgcagc gatgcagctt ttgatgaaaa cagatggtta tccagcgatt gcacaaggag
    28141 cagtcacaac tggattagtc tatttgattg ttgcgctgat catcaaaaag atcggttcag
    28201 catggctgga taaaatcttg cctccaatcg tcgtcggtcc agtcatcatg gttatcgggt
    28261 tgggacttgc ggcaaatgca gcaacaaatg ctatgtataa tcagggcacg tatgatttca
    28321 agtatattgc tgtagcgctg atcacattag gattaacaat tttcttcaac atgtggctaa
    28381 gaggttttct cggattgatt ccgattttac taggtattat tagcggatac atcgtagcct
    28441 tgctgtttgg aatcgtggat actaagccaa tcgtagacgc tgcctggttt gctgttccta
    28501 actttgagat tccctttgta caatatgaac caaagctata tcttggagca atcacaacaa
    28561 tggcacccat cgcttttgtg acgatgacag agcatatcgg ccatcttatg gtattgaata
    28621 aactaactaa acgtaatttc tttgaagaac cgggtcttca taagacgttg acaggtgatg
    28681 gattagcaca aatcgtggca gcctttgtcg gaggtcctcc tgtaacaagt tacggtgaaa
    28741 acattggtgt tctggctatc acacgtgtcc atagcgtgtt tgtgatcggt ggagctgcag
    28801 tatttgcagt cattttagga ttcgtaggaa aattaagcgc tttgatttta agtatccctg
    28861 gaccagtcat ctcaggaatc agttttatct tatttggagt aattgccgca agtggaatga
    28921 agattttaat cgagaacaaa atcgattttg ataaaaagaa aaatctgttg attgcttcag
    28981 ttatcttagt agtaggaatt ggcggactcg tctttgaagt aggcacattt acattgtcgg
    29041 caatggcatt agcaactgta cttggcatca ttttaaatct tatcttacca gaaacttcac
    29101 gaagcgaaga aaactaagga ggaaacatta tgatcatcaa atcagaacgt atcagcttaa
    29161 agcatttatt gaccgttgag gcacttactg atcaagaagt tatgggcttg atccgcagag
    29221 ggcaggaatt caagcaaggt gctaattgga ctccgcaaaa gcgccagtac tttgctacaa
    29281 acctcttttt cgaaaatagt actcgcacac ataaaagctt cgatgtagcg gagaaaaaac
    29341 tagggctaga agtgattgaa tttgaagcaa gcacaagctc tgtccaaaaa ggggaaacac
    29401 tatacgatac agtactgacg atgtcagcat taggcgtaga cgtagcggtt atccgtcatg
    29461 gtgatgagaa ctattatgac gaactgatac aaagtaaaac aatccagtgt tcgattatca
    29521 atggaggaga cggaagcgga cagcatccaa cccaatgctt gcttgatttg atgaccattt
    29581 atgaagaatt tggtcatttt gaaggtctga aagttgccat cgtaggtgat atcactcact
    29641 ctcgcgtagc taaatccaat atgcagatgt taaaacgtct aggcgcacaa gtattcttct
    29701 caggtcctag agaatggtac gacgatgaat tcgaagtgta cggacattat atgccattgg
    29761 atgaactact agatcaagtt gatgtcatga tgatgcttcg ggtgcaacac gaacgccatg
    29821 atggaaaaga aagtttctca aaagaaggct accatcagga acatgggtta acagttgagc
    29881 gtgcgaagaa gttgcaaaaa catgcaatca ttatgcaccc agcaccagtt aaccgtgatg
    29941 tagaattagc tgattcatta gtggaaggat tacagtcgcg gatcgttcag caaatgagca
    30001 atggagtatt cgtcagaatg gcaatcttag aagcagtgct aaatggaaaa gcgtaattat
    30061 aggaggatga aaaaatgaaa acactcatta aaaacggtca gatcaataca cgaaaaaata
    30121 tgacgacacc agcagaaatt tggatagaag atgggcgaat caaagcaatc ggtacaggat
    30181 tctcagaagc cgaatttgat gaagtgtttg atgcaaaggg acaattgatc acacctggtt
    30241 tagtggacgt ccatgttcat ttgagagagc caggatttac atacaaggag acgatcgaag
    30301 ctgggactcg ctcagctgct cgtggcggtt tcactactgt atgcgcgatg ccgaatttaa
    30361 atcctgtacc tgatacggca gaaaagctga gacaagtata tgacatcatt agaaaagatg
    30421 cagttgtgaa ggtattgcag tatgcgccaa tcacagaaaa tctgcgaagt gagaaattag
    30481 ttgatcaaga agcactgatt gaagaaggtg cttttgcttt cacaaatgac ggtgtaggtg
    30541 tccaaacagc tgggacgatg tatctggcta tgaaagaagc tgcaaaaaac aataaagccc
    30601 tagtagccca tacagaagat gaatctttat tatttggcgg cgttatgcac gctggcaaga
    30661 aggcagaaga acttggtctt cccgggatct taagcgtaac cgagtcttct caaattgcta
    30721 gagatctgtt gctagctgaa gcaactggtg ttcattacca tgtttgccat gtttctacta
    30781 aagaaagtgt ccgtgtaatc agagacgcaa aaaaagcagg aatccacgta acagcagaag
    30841 tttcacccca tcatcttatt ttgatcgacg aagatattcc cgaagatttc ggcttttgga
    30901 aaatgaaccc gccgctaaga ggacgggagg acagagaagc tttgatcgaa ggcttgcttg
    30961 acggtacgat cgattgcatc gctacagacc atgcccccca tggattagaa gaaaaaagcc
    31021 aaagtttcat gaaatcacca tttggtatcg taggaagcga aacagcgttt caactgatct
    31081 atactcattt tgtagaaact ggtcgattca cgttggaaca agtgatcaat tggttggcag
    31141 ttaaaccggc tgagattttt ggtttgaatg caggaacgct aacggttggt gcccccgctg
    31201 atgtagcagt ttttgatatt acacaaactt gcacgatcga taaagaagac tttttatcaa
    31261 aaggggagaa tacacctttt atcggctgga aagtaaaagg cgaaacacaa atgacttttg
    31321 tcaacggaaa acttgtgtgg cagaagggag aataaaagta tggaacgttt gcttatatta
    31381 gaagatggaa cagttttcga aggaaaggct tttggtgcag atgctagtgt cttcggagaa
    31441 atcgttttta caacaagtat gactggttat caagaaacta tcactgatca aagttttaac
    31501 ggtcaaatca ttacatttac ctatcctatg gtgggtaatt atggggtaaa tagagatgat
    31561 tatgaatcga tttctccaac atgcaaagga gtagtagtaa aagaacatgc acgtgtagct
    31621 tcaaactggc gaaaccaaat gacgctagat tcatttttga aacaaaaagg aattcctgga
    31681 atatcaggta tcgatacgcg cgcccttact cgcaaaatcc gtgaagtcgg aacgatgaaa
    31741 gctagtatag ttgatgctgg agattccttc gaacatgcat ttgatcagct gaaagcaacc
    31801 gttctagcaa ccaatcaagt gcagcaagtc tcaactaaca aaccttatcc aagtccagga
    31861 acaggtcgga aagtcattgt cattgacttc ggcttgaaac atagtatttt acgagaattg
    31921 agtaaaagaa actgtaacct cacagtactt ccctataaca cagatgccga gacgatttta
    31981 gcgctatcac cagacggagt catgctgacg aatggacctg gtgatccaaa agatgtgcca
    32041 gaagcaatcg aaatgatcaa aaaaatccaa ggaaaagtac cgatttttgg tatttgtctc
    32101 ggtcaccagt tatttgcttt agccaacggt gcagatacct ataaaatgaa attcggacat
    32161 cgaggattaa atcacccggt acgagaagta gcgaccggaa gaattgattt tacttctcaa
    32221 aatcatggct atgcagtcga tgaagccacg attgatccag aaaaactcat cgtaactcat
    32281 gtagaagtaa atgacggaac agtagaagga gtcaaacaca gagattatcc cgctttcagt
    32341 gtgcagtttc atccagatgc tgcccctggt ccgcatgatg cactacattt atttgatgaa
    32401 tttatggaaa tgatggacgc aggaaaggag cagtaaacag atgccgaaac gtacagatat
    32461 caagaaaatt atggtgattg gatctggtcc gatcatcatt ggtcaagccg cagaatttga
    32521 ctacgcagga acacaagcct gccttgcctt aaaagaagaa ggatatgaag tggttttagt
    32581 aaattctaat cctgcgacga tcatgacgga taaagaagtg gcagatcaag tatatatcga
    32641 accgatcaca cttgaatttg tatcaaggat tcttcgaaaa gagcgtccag atgctctcct
    32701 tccaacttta ggcggacaga caggcctcaa tatggcaatg gagctggcag catcaggtat
    32761 tttagaagaa ttgaacatag aacttctagg aacaaagctt tctgcgatcg atcaagcaga
    32821 agatcgtgat ctattcaaac agttgatgaa agaattgaat cagccaatcc ccgagtctga
    32881 aatcgtcaat acagtagaag aagctgtaga ttttgcaaat aaaatcggct atcctgtcat
    32941 cgttcgtcca gcctttactt taggcggaac tggtggaggt atgtgtgaaa atgaagaaga
    33001 attacgtatc gtatcagaaa atggattaag tctttccccc gctacccaat gtttgattga
    33061 acgaagtatt gcaggtttta aagaaattga atatgaagtg atgagagatt cggctgataa
    33121 tgcaatcgtt gtctgcaata tggaaaattt tgacccggtg ggtatccata caggtgattc
    33181 tatcgtattt gcccctagtc aaacattgtc tgaccatgaa tatcaaatgc ttcgtgatgc
    33241 ttctctttcg attattcgag cacttaagat cgagggaggc tgtaatgtcc aactcgctct
    33301 tgatccacat agttttaatt attatgtgat tgaagtcaat ccgcgtgttt ctcgttcatc
    33361 tgcgttagca agtaaggcga ctggctatcc gatcgctaag ctagctgcta aaatagcgct
    33421 tggattaaca ttagacgaaa tgaaaaatcc tgttacagga acgacttttg cagaatttga
    33481 acctgcatta gattatgttg tagctaaaat tcctcgctgg ccgtttgata agtttgaaaa
    33541 cggagaacgg cttttaggca cgcagatgaa agcaactggt gaagtgatgg ctatcggtcg
    33601 gaatatcgaa gagtctttat tgaaagctgt gcgttcttta gaaatcggta cacaccatat
    33661 cgaacttcct gagttggcag acatcacgga cgatgaccta atccaaaaag tggttcgagc
    33721 acaagatgat cgcctgtttt atgtagctga agctattaga agagggtaca ccatagaaga
    33781 attagcggat ttgacaaaaa tcgatttatt ctttttagat aaactgttgc atatcgtaga
    33841 aatcgaacag cagttgaaac agcagccgtt ggatgacaag atattgaaaa cagcgaaaca
    33901 aaacggtttt acagacaaaa aagtggcgga gctgtgggaa ataaacgaag aagccgttcg
    33961 ccagatgaga aaagaaaatg atttgattcc tgtatataag atggttgaca cttgtgcagc
    34021 ggaattcgaa tcaaaaacac cttattttta tagttcctat gaatatgaaa atgaaagttc
    34081 acgatcagaa aagccctcaa ttctagtact gggatctggt cctatccgta tcggacaagg
    34141 ggttgaattt gactatgcga ctgtgcattc tgtcaaagca atccagcagg caggatacga
    34201 agcaatcatc atgaacagca atcctgaaac agtttcgact gacttttcta tttcggataa
    34261 attatatttt gagccattga ccttcgaaga tgtgatgaat gtgatcgagc tagaacagcc
    34321 gataggagta atcgttcaat ttggtggtca gacagcaatc aacttagccg aacctttgac
    34381 tgctgctggt gtaaaaatct tagggacaac gatcgaagac ttggatcgtg cggaaaatcg
    34441 tgatttattc gagcaagcgc tgaaagagtt ggatattcct caacctttag gggatactgc
    34501 tacaagtaaa gaagaagccg tggttatcgc ttcgagaatt ggttatccag ttcttgttcg
    34561 tccaagctat gtattaggcg gacgtgcgat ggaaatcgtt gaaaaccagc gagatttaga
    34621 agattacatg gaacatgctg tgaaagcatc tccagaacat ccagtactag tggaccgtta
    34681 cttgcttggt ggtgaatgcg aagtggatgc gatttgtgac ggtgaaacag tactcatccc
    34741 agggatcatg gagcatattg agcgtgctgg tgtccattca ggggattcaa tggctgtgta
    34801 tccacctcaa acattatctg aatcgatcaa gaaaacgatc gaagattaca cgatccgctt
    34861 agcattaggt ttgaattgtg tcgggatgat gaatatccag tttgtcatcc atgaaaatga
    34921 ggtttatgtg atcgaagtca atcctcgagc aagtcggact gttccattct taagtaaggt
    34981 gacaggaatt ccaatggcac aaattgcgac gaaagctatt ttgggagaga aactaaagac
    35041 acttggttat gaaaacggac tttatccaga aacaaaagaa gttcatgtta aagcacctgt
    35101 cttttctttt acgaaattgc agaaagttga tacttactta gggcctgaaa tgaaatcgac
    35161 tggagaagta atgggatctg acagacaatt agacaaagct ttgtacaagg cattcgaagc
    35221 ttctggatta cacttgcctg aatatggagc cgtattattt acgattgcgg acgaaacaaa
    35281 agaagaagca ctagaattag ctaaacgttt tacagaaatt ggctatagtc tgataggaac
    35341 gaaacataca gctgcttatt tcgaaaaaaa tggtctggtc gttgaaacag tagcgaaaat
    35401 ttctgaggaa gcagcagaaa aaaatgtagt cgacttaata agagatggta aaacacaagt
    35461 ggttgtcaat acgattgata aagatcgggg aaatgcttct aaagacggtt tcatcatccg
    35521 aagagaagca gttgaacatg gtattccatt gtttacttct ttagacacag cagatgcgat
    35581 cgttcgagtg atggaatccc gtgctttttc aactaaccct atttaacggc gagcacatgt
    35641 taagacaaaa agacgaaaaa ccgttgcagg aggggacaga tgaaacaaga aatgatggaa
    35701 atcgttcagc aaaaacagct ggcaccaaag atttttgaaa tgacattgaa aggtgatcta
    35761 gtaaaagaaa tgcagcaacc aggacagttc ttgcatattc tcgtgccgcg tgcagacttg
    35821 ctattacgcc gaccgattag cttgaatcat atcgaccacg aaacaaatac ttgccgtatc
    35881 atttatcggg tggaaggtga ggggactaga gtcttctcta ctctttctgc cggagaccat
    35941 ttagatgtca tgggtccttt aggcaatggt tttgatttgt ccaatcttca tacaggagac
    36001 gaagtattta taatcggtgg aggaatcggt gtccctcctc tatatgagtt atcaaaacag
    36061 ttagtcgaaa aaggaattaa gccaacgcat tttttaggtt ttgccacaca tgaagtcgtt
    36121 tattatgaaa aagaatttaa tcgtctagga gaaacaagaa tcgctactga cgatggaaca
    36181 tacggtatgc acggaaatgt cggtaattta ttgttaggaa cgaagacgga acctacagct
    36241 gtttttgcat gtggttcaaa cgggttattg aagacagtgg aacagctgtt ttcttcgcat
    36301 caaaatgtcc agttatcatt agaatcccgc atggcatgtg ggataggggc ttgctatgct
    36361 tgcgtttgtc atacgccgga agatgaaaca ggaacaaaaa gtgtgaaagt ttgtgacgaa
    36421 ggaccgattt ttaaaatagg agaggtaatt atatgaatga gaatccttta gcggtcaaac
    36481 ttcctggcct agaattaaaa aatcctatca tgccggcaag cggctgtttc ggttttggca
    36541 aagaatacgg aaaatactat gatttgaatc agcttggatc gatcatggtc aaggcaacga
    36601 ctccaaatgc ccgctttggc aatcccacgc caagagtggc ggaaacacca tctggcatgt
    36661 tgaatgcaat tggtttgcaa aatcctggtc tagacgttgt tatgcataaa cttcttccag
    36721 aattagagga gtacaatgag ttacccatca tcgcaaatgt tgctggtgct tgtgaagaag
    36781 actatgtaga agtttgtagc aaaattggcg aagcaccaaa tgtgaaagca atcgagctga
    36841 acatttcttg cccaaatgtc aaacatggcg gaattgcttt tggaacagat tctgatgttg
    36901 cttttcaatt aacacaagca gtaaagaaag tttcttctgt tcctgtctat gtcaaattgt
    36961 ccccaaatgt aacagacatc gttccaatcg ctcaggcaat cgaagctgga ggggcagatg
    37021 ggtttacgat gatcaatact cttcttggta tgcggattga ccttaagaca agaaaaccga
    37081 ttttagccaa tcaaacaggt ggactctccg gaccagcaat caagcctgtc gctattcgtt
    37141 tgattcacca agtagcaagt atatccagcc ttccaatcat tgggatgggc ggcgtgcaga
    37201 cagtggatga tgttcttgag atgtttatgg ctggagcaag tgctgtagct atagggaccg
    37261 cgaatttcac cgatccgtat atctgtccta aactaattaa agagctccct gtgcgaatga
    37321 gcgaactagg catcgaatcg ctggaaagat taatcaaaga agtgagagag gaacgtgaac
    37381 gatgaatcaa ctagaccaac gaccaattat tgcattagat ttttcaacta ggcaagaggt
    37441 agaagacttt cttcgatttt tcccgaaaga agaaaaattg tttgtgaaaa tcggaatgga
    37501 attgttctac caagaaggac ctgaaatcgt acgttatcta aaagaggctg gtcataatgt
    37561 atttttagat ctgaaacttc acgatattcc gaatacggtc gaaaaagcaa tgcggggatt
    37621 ggcaaagctt ggtgttgatc tcacctgtgt tcatgccgct ggcggtattc gtatgatgga
    37681 ggcggcaatg cgaggattag aagaggggac tccagaaggt ggaaaacgtc cgctacttct
    37741 agctatcact cagcttactt ctacaagtga agaagaaatg catgccgatc aactaatcga
    37801 agtttcttta gaaaaaagtg tgatccatta tgctagctgt gccaaaaaag caggattaga
    37861 tggtgtggtt tcttctgctt gggaagtaga agcgatcaaa gaaatggcag gagacgaatt
    37921 tgtttgctta actccgggta taagaccaga aggaacagtc gcaggagatc agacaagagt
    37981 agtcacacct tctcaggcaa aaaagatcgg ctcaactttt atcgttatag gtcgaccaat
    38041 cactcagtca actaatcctt ataaagcata tcaaacgatt caaacagaat ggagctaacc
    38101 taaaatgaat gttgaacaaa gtatcgcaaa agacttatta gaaattgaag cagtattttt
    38161 aaatccaagt aatcctttta catgggcaag tggaatcaaa agcccaattt actgtgataa
    38221 ccgaatcacg atgagctatc caaaagtccg aaaagagatc gcaaaaggat tagcaagcaa
    38281 aatcaaagaa gcttaccctg aagtacaagt gattgctgga actgcaacag caggaatccc
    38341 tcatgccgca tgggtagcgg aaatactgga tttgccaatg gtctatattc gaagtaaggc
    38401 gaaagatcat gggaaaggta accaaatcga aggacgtata tttgaaggtc agaagatggt
    38461 tgtgatcgag gatttgattt ctacaggcgg aagtgtcttg gaagcagctg aagcagcaaa
    38521 aagagaaggt gcggatatcc ttggtgttgc ggctatcttt acgtatgagt tgccaaaagg
    38581 aaaagcaaat ttcgaaaaag ccgaaatccc tcttatgacg ttgaccaatt attctgtatt
    38641 gattgaagca gctttagaag atcgttatat cgatgagcaa gaattaactc tgctaaaaga
    38701 atggaaaaaa gatcctgaaa attggcagat tgaagcgcgc ttgatgaaga tagagaaatc
    38761 cgatttgtgt ctccagaatc atcaattgga atagcaaaaa tccaaaaact gattgcgaga
    38821 agcatctttt catcaggtat gaaaagaaac ttctatcgtg atcagttttt gattttttta
    38881 aaattcattt ttctaagact ggttagcaga aagaagagaa taatctcttc tcttttttct
    38941 attatatata gattttctct attttttggt aagggagctg tttcaaaaat ggtaaagagc
    39001 ggaaatgttg cgaatatcag ttatttaatg atatgatatt cggaaaaaga aatctcaaag
    39061 ggaggtaact tttatggggt tgaatgaatt tattttagga ttgaacaatt tagagacgat
    39121 cacaagggca cctggatttt ttaaatatac agagcataca gttgcagcac attcgtatcg
    39181 tgtagcatct attgcgcaag ttctaggaga cattgaagaa gcgaatggac aaccgatcga
    39241 ttggaaactg ctctatgaaa aatcattgaa tcatgactac acagagcgtt ttattggtga
    39301 tatcaaaacg ccggtgaaat acgcaagcaa agagttaaga gggatgctgc aaacagttga
    39361 agaaaagatg acggacgaat tcatcaacca agagattcca gctgagttcc aagaagttta
    39421 tcgccgtcgg ttgttcgaag gaaaagatga aaccgtagaa ggagaactat tagccatttc
    39481 agataaggtt gaccttcttt atgaatcttt tgatgaaatc atcaaaaata acccggaaaa
    39541 agtttataag gagatgttca ttgaggcagt gactacgatt aaaagctttg aacatcggcc
    39601 ttctgttcag tatttcttta aagagatatt tccggaacta ttgaaccagg aattttatgg
    39661 aaaagaggcg tttttagaaa ccgtctcaac gattgtaaag aaagaagaaa aataaaaatg
    39721 atatgagatg cagggggaag tatagttgaa aaagaatatg gatgtcgaat ggggctattc
    39781 tggagacaaa ggtccggaaa attgggcgag tttatgtgat tggtttgcga gaggtgcgga
    39841 atttccattg cagtcgccta ttcatttgac caagggggaa gaaaccaaaa tagaaggaga
    39901 cacgctttcc tttcattatc aaaagcaacg gtttactgac aaggaattca aaaacacgat
    39961 tcatttagta ccttttgacc aactcagtta tgtagagttc aagaaagaac gctattattt
    40021 gacagatatt catttccact tgccttctga acatatcatc aacggaaaac aggaagatat
    40081 tgaatttcat tttgttcata tgaacagcaa aaaagagaat ctagttgtgg gtgtcatgta
    40141 ccagctaatg gtacaggaag gttggctgta taatcaagaa aatggggaaa gttggaactt
    40201 agaaaaacat gagcattggg taaatccgga tgtgttcttt ccggagcaaa aaagccattt
    40261 ccattatatt ggctcattga caactccgcc taccagcggg ccaatccaat ggtttgtaat
    40321 ggatcatgta ggaaaactga accaagagtt tcttctcccc ttcgatggtc agtatgctcg
    40381 accgaacaac cgaccaatcc agcccttgaa tggaagagaa atttattatt tcgaggagtt
    40441 tgagcaatga atattcagca gatgaaatac gtggtcgctg tagcaaacaa tggtagtttc
    40501 agagaagcgg caaagaaaat gtttgtctcc cagccaagtc tttcagctgg tatcaaagaa
    40561 ttagaaaccg agttagggat cacgctgttt actcgaacga atcgaggggc atatttaaca
    40621 gaagaagggc aagacttctt aaatcgtgcc gagaaaatat tggtccagct tgatacatta
    40681 gaaactcact atctaacgag tgaaaagaga gacaattttt cgatcgctgc ccagcattat
    40741 gatttcttag gtcctttgac cgcacagcta ctcgaggaat tcaaagacga gatcaaacaa
    40801 tttcggatca tggagacgac aacagcgaga gtgatcgaag aagtcaaaga gcagcacagt
    40861 gagatcggca ttatttatat gaatgagcat aatcaagctg gtatcagaag atatttacaa
    40921 caaggagagc taagtggccg cagcattgga aaattcaaaa cgcatatttt tttaaggcaa
    40981 ggacatccat tagctgaaca cgcagagatc accaaagaag aattgttatc ttatcctcaa
    41041 gttcgtttta cacaggatgg aaataatttc ccgtattttt atgaagacct tatcgagatc
    41101 cctgatcaag aaacagtgat ttatacgagt gatcgaggta ctttgatgaa tatcgttctt
    41161 gaaactgatg cttacgcttc aggatctggt attgtgattg gagggattcg cgagcacttg
    41221 cggttaatcc ctttggcaga aggcgaacta aatgaatttt atatcattta ttcggcgaaa
    41281 cgtccgctca gtgaaatcgc tcaaagattt atcaaagagc ttactcggtt actggaccag
    41341 cagagctaag aaaagcagat gagaacgctg tttattgaac accgttgatc aaaaaagagg
    41401 ggttgtgaca gaattttgtc acaactcctc tttttgtagc aaacaattta taactaattg
    41461 ctgttgtggg aaagtacgca aatatgattc tagaaacact cattgattac ctgttttgtt
    41521 tcatagctgt aattttttct tcttctggtg tcacgataat cgtcttttgg ttttcataaa
    41581 taacataacc aggcttggcg ccatttggtt ttcggatatg ctttacctgt acaagatcaa
    41641 ccggcacttg ggcagagtat cggtattttg aaaaataggc agctaactcg gcagcttctg
    41701 taattgtttc atctgagggc ttgtcacttt taatgatgac gtgcgaaccg ggtatatttt
    41761 ttgcatgaag ccaatagtcg gtttttttag ctgtcttcat tgtcagctgg tcattttgaa
    41821 gattgttttt accaacaaga atgagcgttc cgtcagaaga aaagtattga tcaggctgag
    41881 atggtttctt acgtttttgt ttttttaagt tcttcttttt caggtatcct tctgaagtca
    41941 attcttcttt aattgcttcg atatccattg ggccggcaat ttccagttga gataagacag
    42001 attcaagata ttggatttca tctttggctt cttggatttg ttcgccaatc agcttaacag
    42061 cgttcttcag cttttggtat cgatggaaat atttctgtgc attttgattt ggtgtcaatg
    42121 ccggatctag tgctattttg attggccgat cttcttcata ataattaggt aaaacaactt
    42181 cttttgcgcc gcgtggtact tgtgtcagaa aagtagtaag tagttcgcca tttctacggt
    42241 aattctcagc gttttcggat tctttgagtg tttgttcacg ttttttcaat ttatttttat
    42301 ttcgttttag ttcattttcg atttttcgaa tcagttctcc gccttgttgt tttgcccgat
    42361 ctttctctgc tttttcgtga taatagctat ctaataattg actgagtgtt ggataagctg
    42421 ttacagcaga tgcctgttcg gacaatacct gataagcaat cggtgtgaaa aaatctttat
    42481 tttccgtttc gtaaaaagta ggaataggct gatggctgat ggctgagaaa aattcgtccc
    42541 atacaaccat cttttcatta ggacgttcca gtaatctttt ggtcagctct tgagcgatat
    42601 cgaaaccgat tccctgaaac tgacgttgga ttgcttttgg atctagttca gtttgggaaa
    42661 gccgctgaaa gattttttct ttctcggagc taaatgggtt caagacctct tgttttggcg
    42721 gagcaacata ttctacacca ggaagcaaag aacgataagt gttttgagag cttccaatgt
    42781 gtttgatcgc atccagtatt tttccagttt ctcgattaac taagacgatc gtactatgac
    42841 gtcccattaa ttcaacgatc aatatgatat tttgtagatc acccaactcg tttcgtttag
    42901 caaagtggaa atggatcact cgatcatttt cgatttgttc gatgtcttct aaaatagcgc
    42961 catccaaata ttttcgaagc atcatcacga agttaggcgg attatcagga ttctgataat
    43021 cgatttgggt aatctgtaca cgagcataac ttgggtgcgc agataataat aatcgatggt
    43081 ttttgccgcg tgaacgaatc accagcacga tttcattttc ataaggctga tggattttcg
    43141 agatccggcc agatagtaac gtttcacgca attcgttgac catcgcgtgg gtaaatacac
    43201 catcaaagga catggttcat tcctcatttc ttttcataag tatattcatt ataacgaaaa
    43261 aaagaagaaa aaccaatttg gccgaatagt aaagcaaaag tctagaaaaa tcttggacta
    43321 attacataaa aaacagtgtt tctatcgttg aaatccaaat ttttaggt
//
LOCUS       DAYYFU010000019        41159 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000019 DAYYFU010000000
VERSION     DAYYFU010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.019"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(448..1230)
                     /locus_tag="ACHA34_001211"
     CDS             complement(448..1230)
                     /locus_tag="ACHA34_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791866.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1295..2131)
                     /locus_tag="ACHA34_001212"
     CDS             complement(1295..2131)
                     /locus_tag="ACHA34_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8791867.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2149..3558)
                     /locus_tag="ACHA34_001213"
     CDS             complement(2149..3558)
                     /locus_tag="ACHA34_001213"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8791868.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3572..5395)
                     /locus_tag="ACHA34_001214"
     CDS             complement(3572..5395)
                     /locus_tag="ACHA34_001214"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8791869.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(5675..5902)
                     /locus_tag="ACHA34_001215"
     CDS             complement(5675..5902)
                     /locus_tag="ACHA34_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791870.1"
                     /translation="MIEIGYCLLIFTKKTSITIVAYIVGMLFIPSFTHQIHQFFPRAE
                     LLKLCDPLYAFLMLSRFWEFPAGYVGMVLLV"
     gene            complement(6238..6564)
                     /locus_tag="ACHA34_001216"
     CDS             complement(6238..6564)
                     /locus_tag="ACHA34_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791871.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6610..6972)
                     /locus_tag="ACHA34_001217"
     CDS             complement(6610..6972)
                     /locus_tag="ACHA34_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFM8791872.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(7009..7224)
                     /locus_tag="ACHA34_001218"
     CDS             complement(7009..7224)
                     /locus_tag="ACHA34_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8791873.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7351..7686
                     /locus_tag="ACHA34_001219"
     CDS             7351..7686
                     /locus_tag="ACHA34_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8791874.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7762..9759)
                     /locus_tag="ACHA34_001220"
     CDS             complement(7762..9759)
                     /locus_tag="ACHA34_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791875.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9857..10132)
                     /locus_tag="ACHA34_001221"
     CDS             complement(9857..10132)
                     /locus_tag="ACHA34_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8791876.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10364..11098
                     /locus_tag="ACHA34_001222"
     CDS             10364..11098
                     /locus_tag="ACHA34_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8791877.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11137..12279
                     /locus_tag="ACHA34_001223"
     CDS             11137..12279
                     /locus_tag="ACHA34_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8791878.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12374..13765)
                     /gene="sufB"
                     /locus_tag="ACHA34_001224"
     CDS             complement(12374..13765)
                     /gene="sufB"
                     /locus_tag="ACHA34_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFM8791879.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13770..14240)
                     /gene="sufU"
                     /locus_tag="ACHA34_001225"
     CDS             complement(13770..14240)
                     /gene="sufU"
                     /locus_tag="ACHA34_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFM8791880.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14227..15462)
                     /locus_tag="ACHA34_001226"
     CDS             complement(14227..15462)
                     /locus_tag="ACHA34_001226"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFM8791881.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15459..16745)
                     /gene="sufD"
                     /locus_tag="ACHA34_001227"
     CDS             complement(15459..16745)
                     /gene="sufD"
                     /locus_tag="ACHA34_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFM8791882.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16758..17531)
                     /gene="sufC"
                     /locus_tag="ACHA34_001228"
     CDS             complement(16758..17531)
                     /gene="sufC"
                     /locus_tag="ACHA34_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFM8791883.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17728..18576)
                     /locus_tag="ACHA34_001229"
     CDS             complement(17728..18576)
                     /locus_tag="ACHA34_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8791884.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18600..19286)
                     /locus_tag="ACHA34_001230"
     CDS             complement(18600..19286)
                     /locus_tag="ACHA34_001230"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8791885.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19279..20313)
                     /locus_tag="ACHA34_001231"
     CDS             complement(19279..20313)
                     /locus_tag="ACHA34_001231"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8791886.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20376..20632)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20708..21046)
                     /locus_tag="ACHA34_001232"
     CDS             complement(20708..21046)
                     /locus_tag="ACHA34_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFM8791887.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(21048..21404)
                     /locus_tag="ACHA34_001233"
     CDS             complement(21048..21404)
                     /locus_tag="ACHA34_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFM8791888.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21410..22597)
                     /locus_tag="ACHA34_001234"
     CDS             complement(21410..22597)
                     /locus_tag="ACHA34_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8791889.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22788..23633)
                     /locus_tag="ACHA34_001235"
     CDS             complement(22788..23633)
                     /locus_tag="ACHA34_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8791890.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23867..24832
                     /locus_tag="ACHA34_001236"
     CDS             23867..24832
                     /locus_tag="ACHA34_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFM8791891.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25209..26252
                     /locus_tag="ACHA34_001237"
     CDS             25209..26252
                     /locus_tag="ACHA34_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFM8791892.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(26314..26778)
                     /locus_tag="ACHA34_001238"
     CDS             complement(26314..26778)
                     /locus_tag="ACHA34_001238"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFM8791893.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            27024..27971
                     /locus_tag="ACHA34_001239"
     CDS             27024..27971
                     /locus_tag="ACHA34_001239"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8791894.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(28182..30254)
                     /locus_tag="ACHA34_001240"
     CDS             complement(28182..30254)
                     /locus_tag="ACHA34_001240"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8791895.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(30187..30564)
                     /locus_tag="ACHA34_001241"
     CDS             complement(30187..30564)
                     /locus_tag="ACHA34_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFM8791896.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(30693..31253)
                     /locus_tag="ACHA34_001242"
     CDS             complement(30693..31253)
                     /locus_tag="ACHA34_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8791897.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            31403..32695
                     /locus_tag="ACHA34_001243"
     CDS             31403..32695
                     /locus_tag="ACHA34_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8791898.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(32755..33873)
                     /locus_tag="ACHA34_001244"
     CDS             complement(32755..33873)
                     /locus_tag="ACHA34_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFM8791899.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(33887..35308)
                     /gene="arcD"
                     /locus_tag="ACHA34_001245"
     CDS             complement(33887..35308)
                     /gene="arcD"
                     /locus_tag="ACHA34_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFM8791900.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(35554..36675)
                     /gene="mnmA"
                     /locus_tag="ACHA34_001246"
     CDS             complement(35554..36675)
                     /gene="mnmA"
                     /locus_tag="ACHA34_001246"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFM8791901.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            37079..37678
                     /locus_tag="ACHA34_001247"
     CDS             37079..37678
                     /locus_tag="ACHA34_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8791902.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(37816..39102)
                     /gene="efmA"
                     /locus_tag="ACHA34_001248"
     CDS             complement(37816..39102)
                     /gene="efmA"
                     /locus_tag="ACHA34_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFM8791903.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(39373..39717)
                     /locus_tag="ACHA34_001249"
     CDS             complement(39373..39717)
                     /locus_tag="ACHA34_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFM8791904.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(39854..40999)
                     /locus_tag="ACHA34_001250"
     CDS             complement(39854..40999)
                     /locus_tag="ACHA34_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8791905.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
BASE COUNT        11848 a         8648 c         7193 g        13470 t
ORIGIN      
        1 ttttataaaa agcaaaaaaa aaaagaaaga aacttattct tgctaataaa ctacgcgaaa
       61 ggagtctaac gttgtttgat ataacttaga ctgatcgtta acttattttt tatatttcta
      121 catagttatc tttagaacca tttcagatac atatgaaaca attacatatg attttttcat
      181 aaaaaaacag tactgtctag tagtataaaa tatacaaatt tcttaaaaat tttttcacta
      241 aaatcactac ctgaaacagt ctaaaacact tatatatcaa cgtttaatga aactatctcc
      301 gtttcatttc agatacaaat aaatatagtc gggctccaaa tccgaaaaaa aataccccca
      361 gtagcttatc ctaagctact ggagatgtat aagtacggtc atataacaac ggggtagggt
      421 aaataaatga aaaagatgga agaatcatta ttcaataact tttttaaata cttcgttgta
      481 atcagttacc tgtccgttct taaataccac agaataatct tttttcccat tttcaagtaa
      541 ccgaagtgga atgtctttac caacttgctc tgtcactgca ttactgatac ctgttagatt
      601 agctaataaa tctgataaag attgagcatc tgtagaaatc tctgtatcag tatttatcgt
      661 gattccttta tcttcattta caagcttcca agtaggtggt atagctgtgt atgtttcgat
      721 gaatgaatca attctttcat ctatcgtctt cccagtatct acttctgtct cattactctt
      781 agatgaatcg attctttcat ccatcgtctg ctccgtaact gcttctgtct cattattctc
      841 accggaaact ctcttcacac ccaaacttat atattgtcca tctttagtgt gcatagttaa
      901 aattcccacg cttttactaa taaattcact ataaccagtt ctttcaatag agacttcaat
      961 gtatttcaca ctatcatact caaaagaaaa atagacatgt aaaagcattt tgttgtttac
     1021 atctacataa taatcctttg actctacttg agaccttcct atacggaata cagctttact
     1081 gtctgatatc tctataccag catccttatt attaatctgc caatctccaa gtagctcttc
     1141 tggcaagcta gtagtttctt gtgaacactt taccacttca ctggtagagg tcgaaacatt
     1201 cacctccgca ctgttagaag aattttccaa ggaatcagga gccttagcac aaccaacaag
     1261 tctttatata ttgtgcagcc gatattttct tcattcagta cacccttgaa atatgcagtg
     1321 ttagataaag cagttcgtcc tgatttgctg aggcacccat ctcttcttga acgtactcct
     1381 tgattttcaa ggccgcccga tactctttcg gaaaacgctg cttcactgaa gccagcaaaa
     1441 aatcatcctt ctccggcaac tcttccttct taatcattcg ggcaataaaa aactgtaaat
     1501 gcgtcacaaa tcttaagtaa agcatcgaat ctttatcaac ctttttttcg ggaaacagat
     1561 atcctagcat agccaagata tcctttagtg ctcttaccga gagcatggtt tcttccatat
     1621 ttgcttgttc actttgggca tttacaatgt gaaacgcaat gtttccagcc tcctcttctg
     1681 gcaactgaac attcattttt ttgttgatca attggatagc atagcagccg atctcaaatt
     1741 cttttgggta atacttttga atttcaatca gcaggcgatt ttgaaaaaca atgccttttt
     1801 gactgcgttc aatggcatgc ttcaagtgat caaccaaggt aaaaaataga cttttattca
     1861 gcttaaagtc aaacttggcg ttggcataat cgataattcg cttgacgcaa ataattagat
     1921 catccggagc agcctcaaac agtttaatgt aatccttttt tagagcatca gaaagtgaat
     1981 ctaatttaaa tactttttgg attttgtcag ccggaaccaa atcgcctttc tttagttgaa
     2041 atccaatccc tttccccatc aaaattactt ctttgccttg cttatctaag caaagaatta
     2101 aactgttgtt taacgtttta attactctca ttagattcac cgttctctct aagctaagcc
     2161 atttcttgcg attacttttt gataccaaaa gaagctttct ttttttattc gttttaattc
     2221 tttaagattc tcatcggttc ggtcaacata tataaaacca taacgcttat tgaagccttg
     2281 atgggtacta acaacatcaa tcgctgacca aggtaaataa ccaaaaacat ctacaccatc
     2341 tgttaaagct gcttgaatag ccagaatatg tttggctaga taatcaattc gatcctggtc
     2401 attaattcgt ctatcttcct caactatgtc gggtgcccca tagccatttt cagtaattaa
     2461 aataggtaaa tcatagcgat cgtacaattt cctcaatgtt aatcgtagtc cagtagggtc
     2521 aactacccag ccatatggcg ttttatctac ataaggattt tcagctgccc ggtagacacc
     2581 ttcttctccc aacatgatct gttgatcccc cgctctagcg gaaacatctg atccgtcatt
     2641 tttgctggca gcgatggtgg cagttgaata ataattaatc gcaataaaat cgggtttacc
     2701 ggaggataaa attgcctgat cctctgcctc aattttagga tataatccac gatcattcaa
     2761 atagttttca aacaaataat tgtattttcc ccgaactgct acatctaaga aagaccagcc
     2821 tctcaatgtt tcccaattat gggctgcaat tgcatcttct ggactggagg tagcttgata
     2881 catagccgtt aaattgattg ctggaccgat ttttccttta agattcaact gatgatacaa
     2941 gtgaattact ctagcttgag ctaataaaac atggtgatta atttgaaaga gttctttttt
     3001 tgaaggcagc tctccacttg gtggcaaacc aatcgctcca ggatgaagga tcatcgtgtt
     3061 ttgctcatta attgtcagcc aatagttcac cttgtcacca tactcagtaa gcaatacctg
     3121 tacatagtcc acaaaataat cgatagttgc tcgatttaac caaccgccct gctcctctag
     3181 aaaataaggt aaatcgaaat ggtacatggt gacaatcggt tcgattccct gattcaggca
     3241 ttcatcaatt acttgatgat aaaaagaaat tcccttttga ttggttttat tattttcaat
     3301 atccataatt cgcgtccaag cgatggaaaa gcgaaaagct tttaagccta attctttaaa
     3361 cagctgtata tcttctttat agtgatggta aaaatccgag gcaacttcaa aattagctac
     3421 cccttctgta tgctgatact tgtcaataat agacggttgt ttcccatcct cctgataggc
     3481 accctctgcc tggtaggcac taatggaggc accccataaa aacttctctg gaaatgctga
     3541 aagctgtttg tattgcacaa aaatcctctc cttaactaat ttgcatcaat ggttcgtgtt
     3601 cctttaagct tccgcttgct tcactaattc ctgcgtaatt ttcactgttg gtcactacca
     3661 caatcactgt atcatcgtag ccagcgtttc taatggcagc gaaatcggcg ttgattaata
     3721 attgtcctgc tgacacctga tctcctgctt ttacaaatac ttcaaagggt tcccctttta
     3781 aattcacggt gtcgatcccc acatggatca ataattcaat accttcttta gaacgtagac
     3841 caatcgcatg tttagattct aaaatagtaa tgacttctcc ctcaacaggt gaataaatgc
     3901 tgttttcttc tggatgaatg gctacgccct ttcccaatag tcccttagaa aagacagcat
     3961 cgttcacttc agaaatagga cacacggttc cttttaccgg attttcaaca atgactgctt
     4021 cggctattgc cgtttgctct gattcatcag aagtatcgtc aaatccaatt agataagcaa
     4081 tgataaaggt tgcaacaaag gcgattacac acgtaataat tgcgtgaaca atattcattg
     4141 gattttctcc aataaaggct ggtaaggctg ctaagccagg ggaaacaaag gcatatctga
     4201 ctaaaccgct caagccagca tacaaaccag caattgcacc accaatcatg gaagcaacta
     4261 atggtttttt cagcttcaag gttactccat agagtgaagg ctcagtaatt cccattaaag
     4321 cagtgatccc agtggacaca gctagctgct tcaactcttt gtttttagtt ttcgcggcta
     4381 cagcaaaggt agcacctgat tgagcaatat tagaagccag cattcctggc ccattgatca
     4441 tttcagaacc cgtactgctc aattgcatag tagcgattgg cgtcattgcc caagctgtac
     4501 ccgtaaccac caaaaatggc tgcaaaccac ccatcaaaaa tggaattaac caagctgctt
     4561 tgccgttaat ccattcagcc cccattgcta taagatcgtt cagcaacgtt ccaaacggtc
     4621 caatgacaac caacgctaaa ggccccataa ttaaaaatac cagcatcggc ttagtaaaga
     4681 atttaataaa cgaaggcgaa attcgatccg caaagggctc tacataggac atgatccaaa
     4741 cagtaaagat aatcggtaaa actgatcccg cataatctgc agctaatacc ggaattccta
     4801 gaaaagaaac ggcatcgcca gtagaaaaaa gctgtgaaat atttggatga agcaaaatgc
     4861 cagcaacagt aatcgccaaa attgggttga catcaaactt tatggctgcc ccataggcta
     4921 atagtactgg cataaaataa aaggctgcat cagcaatagt attcaaaatg atgtaagttt
     4981 gactactgtc tgatagtaca ccagtcaaag tcaagacggc caaaatggct tttaccatcc
     5041 cggcacctgt tatagctgga atcatcggtg taaaggtggt agaaatgaca ctaatcaatc
     5101 ttgaaataat actttcttga ttgtctgaag aggggccctt tttaattgaa gttcccactt
     5161 ccttttgaat ttccttgaag gctgtttgga cttcattgcc aatcaccact tggtattgtc
     5221 caccgctatc caccacacta attacaccaa gtgttttttc taaggctttc gtatcggctt
     5281 tccttttatc tttgagttca aaacgcagtc tggtggcaca atgggttaag ctgaccacat
     5341 tttcttttcc cccaacctgt tgcaatacat ttcttcctac ttctctgtaa tccattattc
     5401 tctctcctta aagcagtaaa aaagaccaag tcaaaccacg ccccaatcaa cattgaagtg
     5461 ttagtttgtc ttggtcttgc ctgcataatc agtaacatgc caataacgtt attttgctgt
     5521 aatccaataa tagcacatta tgatagcgca atcaacaggg aatttaattt tttatctttt
     5581 cgtttgaccg tatagggtaa tataacagtt agatcacatc aactagggta tatctgcgta
     5641 tacttactgc taaaataagt atatctaata ttaactatac cagtaaaacc attcctacat
     5701 aaccagcggg aaattcccaa aatcgtgaaa gcatcaaaaa tgcatataac ggatcacaca
     5761 acttcaacaa ttctgctctt gggaaaaatt gatgaatttg gtgagtaaag gatggaataa
     5821 acagcattcc aacaatatag gcaacaatag tgatacttgt ttttttggta aaaatcagta
     5881 agcaatagcc aatttcaatc attccgagat ataaaggtat cgatagaatg atttgggcta
     5941 gtattcttaa aaattaacta gcaaatgttt aaactgattt atttctaatc tacatgtagt
     6001 aagtatttca attccaccaa ttatcaataa aaataccaaa catatccata ataatttttt
     6061 ctttttcatt tcttttatat tgttataaca gtgaataaca taaaaattcc cgacaatcat
     6121 tgtaatattt ttttgtcacc tgtattagta tttaatttat aggaaactat aaaaactccc
     6181 tagtgaattt acacctttgg gagttcctgt ttggacggta tgcataatac atttaaatca
     6241 atttaacatt aacacctcag catatggtat ctccacatat atacggtctt tctttggata
     6301 gtactcaata tttatgttga tagcacttcc attgtctaat atagctttag cccggtatgt
     6361 gttatctcca ttttcatgag taatcgtcac atcatcacat gatcgattta agttgtactg
     6421 ttcttgtaaa atctgtgtta ccaatggttt tgatgacttc tctaatccag atgtacctgt
     6481 catttttgaa aaaataatac ctataatagc caagacaaca atacctataa tgacaaactt
     6541 cttgttcata tttactttat tcaacttttt atctcctatc tccaaaataa tttgctctta
     6601 tccctcttat cagtacattg acatccctaa aagtacacca aagattgctc cagctacccc
     6661 tgctatcatc aaaatcgtac ccattttctc atctttttct cgatataagt agcccataat
     6721 aacagcaaca gcaccaaata caacaggaat aaatgctaac gatactacca tagaaatcca
     6781 accaataatc aaatacagct tccctgtctc ttgtccatgt acgatttgtt ccttcatttc
     6841 attaattgtc tgctcagtac ttttctttac tgtgtctgtt ccggttgtct catctgtttc
     6901 ttgtagtgct ccacaaaact ggcaatactt actttcatct gttatttctt tgccacattt
     6961 tctacaatac atgtcttctc ctcctaaaat cattcaattg cttattagtt aactgtttac
     7021 tttattaaat acatcagaaa acagctcatt cattggtaca cttaatatct cagaaagcat
     7081 cttcatttga tagggtttag gaatcgttct ccctgtttgc cacgaactta cacattgctg
     7141 tgtaacattc attttttttg ctaactccgt ttgagaaagt cctttttctt ttctgtatcg
     7201 gctgagatta cttacttttt tcatgattct cccctcctgt tttattatat acaacaatat
     7261 gatgtatttc aactcataaa atataaaaaa cttcaaatac cattacaaat acaacaactt
     7321 gttgtactat actgagatgg gggtgtatta atgaaacaga aacaatatgc aaaggtcatt
     7381 agagaaaagc gaaaagctct cgggtggaca caaaaagaat tagctgaaaa aatcttctct
     7441 acacaacagg cagtagcaag atgggaaaat tctgtaacag agcctaacct cgatagctta
     7501 acagctcttt ctagagccct aggaacccct gtaagccatt ttctagacaa tgttgtcgta
     7561 gattatgagg aagagttttt ggctctctat cgttctctta gcactgaaga tgcagttcga
     7621 acaattgact atatgaagct attaaaaagg caagaaaatg aacgcaatca gctattgaaa
     7681 gattgaaaaa taaataaccc accgaacttt aacggtgggt gtattgagat gaaataagat
     7741 taagagctat ggataacctg tctagtttaa tacaattggg tcttttctct tccttactct
     7801 ccgtccatca tctcttgtta cacttgtaaa ttgatacaga ggaactatcc cagctcctga
     7861 taatatcaat tcttttattg tttcatctat ccctgcccct ttcgctaaat atattaaaaa
     7921 actactactt gctttcacat tgtatagttt cttaatatcc ttagaagctc gttcaagagt
     7981 gaatccttgt ttaacaaagt agcctctgtt ttttattaaa gcatttttca cgctgttatc
     8041 cccaacatta cgtaggcgtt cacaaacagt gttaataagt tctttctctg tcattagata
     8101 tgtcccttgc cacgataagc cctgtaaaac ttcatgtgct tcatctaaaa tcaattgttt
     8161 ttccttgctg agttctgatc gggaattttg aggaaatact cttttagcct ctttttcgtt
     8221 aaaacatatc ttaacccatt cataagaaaa atattgatat gtaaaatgaa ttttttccag
     8281 aagtggaagg atatcttcat tcaatttttg aatcacagaa gttgcattaa aaacgtctaa
     8341 tcgatagaca ttgcttctct tatgattaaa gtttcctaac gctttctctt cctttaacct
     8401 atttaggtat tcgagcatct tcaaaggaac atcttcatca tctaattttt ttatcaatcc
     8461 tataaaagtc ataagtgata gtaacttatt acatttatct atgtcataag ttttaccata
     8521 gtaagaactc atttttttag ctaattgttc ccccgacaaa atacaagtca cactttcctt
     8581 actatcatca aaaaaattaa catcagaaag caagacatca tagacacgtc gcatttcctc
     8641 tagataattc cttattgtcc tgaaaagttt gtctttcatt gattcaagag tgtcaaaaag
     8701 tacttttgat tcctctttca attttcgaaa atgtgtagga tacaagtcta ggttctctaa
     8761 tagccaatta aatgtttcta cccgactgtc tttctttaaa attagcatca caactccaat
     8821 aaaattttca ctgatatcac aattactgga atgacaataa aaaatggaat gcccagtttt
     8881 actggtaaaa atactagcag agggattatg atcgttgtga aacagacatc ttaagttttt
     8941 acttgactta agtaatttat tcatatcaat ggttaacaat ctttcataaa tctcattaaa
     9001 agacatgtcg gaggaatcaa acaggcagtc tttaaaccac tgtttcattt cttctttgtc
     9061 gtcatttttt atcaacttaa caaaatcggt ttcattgact agttcatact ctctccctga
     9121 atcactttga caatttttag aacatgtcat ggtaaaatca atcggaagct cagtaatatc
     9181 taacatgttg tcttcattaa ttattatcac tgcatctgac ttatatcctc caaaaaacat
     9241 tctattcgaa gcatttacat tgtcatcaca cccggggatg ttctttttca agtattcgta
     9301 aacagcaacc attttaccat agtcagtaat tgcttctttt aatagaaaga caatcttgta
     9361 tttttctaga ttttcagagt accgaatact tttttgtatc gcaaacgcag aattatgaac
     9421 taaattgata gataaaacct tttctacgct taagtatcta ctatcatctt tactcaaatc
     9481 tactaccttc cccttctcat ctttaacagt gttatcgaca tccagaataa ttactctggt
     9541 ttccacaaaa ctctccttgt cattgccttt attctttgtc ttgcttggaa taaatgattg
     9601 tgatgaattt agtatctcta caaactcatc tcttgtaact tctctatagt tatctaaaca
     9661 cctatctctt acatcagttt tttctttttt tgtaactggc ttctctaata atttttcttg
     9721 atctacataa attggaatat taatcattga tgtttccatt tgtgtttcct ccttataaaa
     9781 ttctgttaaa gactaaaaca tacagataat aaaaacaaac tctttattac tatatgtttt
     9841 agtcttattt gtttactcaa cctttatacc gttccatgaa actatcaata tcagattttt
     9901 taaatctaat gaccgtatca atttttataa caggtaaccc gtattgagaa acatattttc
     9961 taatcgttcc tactgataca cctagatatt cacttgcctg ctgatagtcc atatactcta
    10021 gagtgactcc agaaataatg tcttctagca taggctttag cactgtaatt atctctttca
    10081 cttgttgttc aacttgtata gaaacaatgc tatcaatgat ttgtagagac atatttcccc
    10141 ctcctttcgt gattgcttga acaaattttc taagaattat cttcgtattt tttgctcaaa
    10201 ataaaagtat tttgttatta accgattaat caaacaaaaa atgattacca gttcatcgac
    10261 atacacataa tacctcgata aaaaaggggt gtcaacagct ttgtaaaatt tatttgcaac
    10321 accccttttt tgaggtatac taattttagg aggtgtggtc aatatgataa aacgtccacc
    10381 aataaattat ttagaacgaa aaaagatact cggaacaaaa ataaaagcta taagaaagag
    10441 taaaaaatta acacaaccag catttggtct tatgattaac aacggtcaat taattgacaa
    10501 aaaaactatc tacgaatggg agaagggaac ctatttacca ataccagagc gattatcccg
    10561 aattgctgat ttgggaaata tgagtataga ggagttagtt tgtggcaatg tagaagaata
    10621 tattttggga attattcttt atagagatag cattgtacta gatggtataa catttccaga
    10681 taaaaacctg ttccagcatc ttcgtcaaca gttcccacca gtacactcta atttagatac
    10741 atggctggat agatattcca aattagaacc agagatgcaa gaatttattg ctaacaagac
    10801 ttgtaataaa gttaaaaatg aaaaaattag tcttttcaat atcttaaaaa tagaagaatt
    10861 attcatcaat gctattgtag aagaatttga caataacatt cttttcctca ctagcagtat
    10921 agaagaacta ctagaaagaa tggtagatga atggctacct attcaattga aagacatgtc
    10981 ttatcccgaa gaagctgtga gagaaattac tgataatata aacaagctag aacaaacgat
    11041 ttcatcaatt gggaaaaaat acactaagaa aaaaatgaag ggaggtgata ccatctagtg
    11101 gtaagtactc cttgtaaact aagaggagat gatttaatgg caaaaatatc aaagtacaaa
    11161 aagaaaaacg gtcaagtggc gtggatgtat aaaggacaca ttgccaccga tccacgaact
    11221 ggtgaaaaaa taaatactac gagaagaggt tttagtagca aacaagaagc tcaaaaagat
    11281 tatgacgagt acagacatca aatattatac ggtgttaaaa agaaagcacc tgatatgaca
    11341 tttgaagacc tgtataatga atggataact catcagcgta ccagtgttaa agcttcaaca
    11401 atcgcaatct cagtaagata cgcaaataat caaatacttc cagcatttgg taagcttaaa
    11461 ctatcgaata tctctgtccc atattgtcaa aaagtggtag atgaatggca cagtaaatat
    11521 gaaagttacg attatatgag aaaacagact gcacaaattt tacgctacgg ggttgcaatg
    11581 caatatattg acaataatcc tatggaaaaa acacttcttc cacgaaaaaa agagtatgag
    11641 aaaaatcgta aattttactc aaaagaggag cttaataatt tactggatgc ttttaaagat
    11701 tttggtaaca tgaaacaata cgccttcttc agattattgg catacacagg tatgagaaag
    11761 agtgaagtac tcgctttaca gtggaaagac atagacacgt ttaataaaga actacatgtc
    11821 aataaaactc ttgctgtaga tgaatttggt aaagttataa ttcaatctcc gaaaacaaga
    11881 gcttctagac gagttatctc tttagatact gagactcttt ctatcctcaa caactggaaa
    11941 ttacagcaaa aagaagagta tttaaaactt ggatacaata ctagctctaa agagcaacat
    12001 gtttttacaa cagttaaaaa cactctatac attccaaaca ctgttaatga ttggttaaga
    12061 tatattttga aaaaatataa cttgcctcgt attacacctc atgggtttag acatacacat
    12121 gctagtttat tattagaagc aggagaatct gtcaaggtag ttcaacaacg attagggcat
    12181 gaaaatagta aagtgacatt agacatctat gcacatatta caaataatgc acctaagaaa
    12241 acaggtcaag actttgctga tatgatggct catcagtaaa agagctggtc aaaatctggt
    12301 catttctagt atacaggcaa aacaaaaaag ctagaaccct tatatatcaa gggttctagc
    12361 tcctataaca ggtttaccca acactgcctt ccatctcata actgatcaat cgattgagtt
    12421 cgactgcata ttccattgga agttctttgg tgaatggttc aacaaagccc atgacgatca
    12481 tttcagttgc ttcagattcg gtcaggcctc ggctcatcag atagtagagt tgttcttctg
    12541 agatttttga gactttggct tcgtgttcta aagagacttg gctgttgtgg atctcattga
    12601 atgggatcgt atcggatttc gaccattcgt ccatgatgat cgtgtcacat tcgatatgag
    12661 agatcgaacc cgcactgttt ttggcaaagg ttacttgtcc tcgatagttt acttctccgc
    12721 cgtcttttga aattgattta gagacgatag agctagatgt atttggtgca ttgtggatca
    12781 tttttgcacc cgtatcttgg atttggtttg ctcctgcaaa ggcgatggag agcatggtgc
    12841 ctcgtgcacc ttttccatct aagtaaacac ttgggtattt catcgttgtt tttgcaccta
    12901 ggtttccgtc gatccattca acggttgcgc cttcgtatgc ttttgctcgt ttggttacta
    12961 agttgtacac attgtctgac cagttttgga tcgttgtgta acggcaatac gcatcttttc
    13021 ttgtaaagat ttctacgatg gcggcatgca ggctgttact tgaataagta ggtgccgtac
    13081 aaccttcgac atagtggatg cttgctcctt cgtcaacaat gatcagcgta cgttcgaatt
    13141 gccccgtgtt ttccgcattg atccggaagt acgtttgtaa tggaacatcg actttgacgc
    13201 cttttggcac gtaaataaag gtcccacctg accatacggc tgaattcagc gctgctaatt
    13261 tgttgtcggt tggcggtaca agttttgaaa agtactcttt gaataattca gggtattctt
    13321 tcaaagcaga gtcagtgtca gtaaagacga ttcctaactt ttcgaattca tctttcatat
    13381 tatggtacac gacttctgat tcatattgtg cggaagcacc ggctagataa gctcgttcag
    13441 cttctggtat accaatcttt tcaaatgtat ctttgatttt ttctggtacg tcttcccagt
    13501 cacgagcggg tttgtcactt ggtttttggt aatatttgat ggcatcgaaa tcgatgtctg
    13561 aaaggtcggg gccccatgtt tgcattggca ttttgttaaa tgtttctaga gatttcagac
    13621 ggaattcaag catccattct ggttcgcctt tgattcttga catctcacgg atcacttctt
    13681 ctgtcaagcc ttctcctgta ctgaaaaccg gttcgacatc atcgtgaaac ccgaatttat
    13741 attcttctaa ttctggtacg cccatgttct cactcctctt ccttgtggta atgatggaaa
    13801 gctgcggtac catcgttttc cattgctttt cctagtgctt tccaggcaag tgtcgcacat
    13861 ttgatccgag cgggaaattt agctacacca ccaagcatag cagcatcgcc taattggtcg
    13921 ggatctttga cttcttttcc ttggacaagc tgtagaaagt ctacagttaa atgttctgct
    13981 tcttctattg attttccgat gacggcgtct gtcatcatcg aggcacttgc cgtactgatg
    14041 gagcagccac tgccgtcaaa ggcgatattt ttgatgattc cgtcttttac atcgacttgc
    14101 aattcgatca catctccaca tgtggggttg ttcaattcga tcttgcttgt cgagtcagtc
    14161 aagcttccac gatgatgcgg atgggaagaa tgatccaaga tcacttgacg gtataaattg
    14221 tctaatttag atagtcccat gttggaaaaa ctcctttgtt gccatgattg cttcaactaa
    14281 acggtcggcg tcttgcgttg tgttgtagaa ataaaagctc gcgcgagcag tcgctgatac
    14341 ttcaaggtac ttcaatagcg gttgcgcaca atgatgacct gctctgacgg ctaccccttc
    14401 catgtctaaa gctgtcgcga catcatgggg atgtaaccca tccaaattga atgcaacgac
    14461 tcctgtccga aaattcgggt cttgtggacc ataaacagtc aatccatcaa ttgcttgtaa
    14521 ttttggcaac acataagcgg ctagttcttc ttcatatcga tggatattgt ccataccgag
    14581 tttttgcaaa taatcgattg catggcctaa tgcgatagcg ccggcgatat ttggtgtccc
    14641 tgcttcgaat ttccaaggta gttctttcca tgtactgtcg tataattcga caaaatcgat
    14701 catttcgcca ccaaattcaa caggttccat ttcttctaac cattttcgtt taccatataa
    14761 aacaccaata cctgttggtc cgcacatttt gtgtccgctg aaagcaaaga aatcacagtc
    14821 tagtgcttgg acatcaatgg ccatgtgcgg ggcagattga gcaccatcta ctacgatgac
    14881 cccgccgttt tcatggacaa gttcagctaa ttcttgaaca ggattgaaga cacctaagac
    14941 attggataca tgagcaatgg agacgatttt ggttttgtcc gtaatctttt tcgctgcatc
    15001 ctccatatcc aggaagcctt cttctgttag ttcgatatat ttcaagacag cacctgttcg
    15061 ttttgccaac tgctgccacg ggataacatt agaatgatgt tccatgtaag agagcactat
    15121 ctcgtctcct gctttgatat agcggtcgcc gaaggttctg gctacccaat tcaagcttgt
    15181 cgtcgtacca cgagtaaaca gcacttctgc tgtttctttt gcatggatga acgcccgtac
    15241 tttttctctt gcttgttcat aatcatgggt cgcgcgttct gctaacgtat ggacaccgcg
    15301 atgaacattg gcgttatcgt gttcgtaata gtacgctaat gcctggagta cttgttttgg
    15361 cttttgggtc gtcgctgcat tatccagata aactaaaggt tcatcattga cgtcttgaaa
    15421 taagatagga aaatcattgc gtagcttttt agcatcgatc atccattcaa cttcccttcg
    15481 attacttcga ctaattcttt ccgaacttgt tctacaggga ttgctgttaa gacagaaccg
    15541 aggaatcctc gaatcactaa gcgctctgct tcttctttat gcaatcctct actcatcaga
    15601 tagtacattt cttctggatc aactcgtcct acactggcag catgtccagc agttacttcg
    15661 ttttcatcga tcaaaagaat aggattcgca tcaccacgtg ctttatcaga aagcatcaag
    15721 acacgacttt cttgttgggc atctgcacct tttgcccctt tcaagatgtg gccgatacca
    15781 ttaaaggtca atgttccttt ttctcggatc actccgtgtt gcaaaatatg cccgatcgta
    15841 tgaggggctt tattggttac tcgtgtatcg attccttgga tttgtctgcc cgagctgatt
    15901 gccacgattt tgacttctgc gtgtgcacct tctcctgcta aatctgagtc aaagtcagcg
    15961 atcacatgac cgtcattcat gacaccaatt gcccagtcga cagaagcatc tcgtaaaatg
    16021 tgcccacggc gattcatata agaagtaata ttttctccta attgatctac ggctgaatat
    16081 ttgatttttg aaccagcttt agcgatgact tccacgatga tattacctga tgattttgct
    16141 acttgttctt tggttgtctg gaaacgttcc agataagaaa attcactatg ttcatctgcc
    16201 acgatcaaca catgcttgaa gaagtgctca tcggatgcgc catcttgaat gaataaggac
    16261 tcgagtggtt catcgatcac gacattttta ggcacataca ggaaaatacc gctgttcata
    16321 aaagctacat ggagtgcagt caattggtct tcgttcatgt ttactgcttt tttcatataa
    16381 tattcttgga ctagttctgg atattcgatc attgcagtga acaagtcggt aaaaatgact
    16441 cctttttctg ctaattccac aggcagttgt tcaaaaatcg tccatgaacc ttgttggaca
    16501 agcacaggat tgtctttcat tgcatcaaat gccggaatcg tgcctgtttc actgatggtt
    16561 tgtgtttctt tcacatcaaa taacgaccag cgatggaatt tcacccgatc gatatgcggc
    16621 aatggcagtt catctgcttt cgctagtgcg tcttgacgga aggttctcat ccaatcaggt
    16681 tcgcctttac ttaaagaaaa atcatttacc gcgtcaagat aatcgagcca gtttttttct
    16741 ttcattcttg ctccccctta cgcttcttct tctttatagt cgattcctaa ttctttactg
    16801 ataccagcgt agccttctgc ttccagacgt ttagctaggt cagcattccc tgtcatgact
    16861 actcggcctt ccatcatgat gtgaacgaca tcaggtgtga tatagttcaa taatcgttga
    16921 tagtgggtga tgatcaatgc accgaaattg tctccgcgca tttcatttac tcctttagca
    16981 acgactttca aagcatcgat atccagacct gaatcgattt cgtctaaaat tgcaaatgta
    17041 ggttctaaca tcaacaattg caggatctca ttccgtttct tttctccgcc agaaaaacct
    17101 tcgttcagat aacgttcagc catttcttct ggcatattta gtaaagccat tttttcatct
    17161 aactttttca aaaatgccat aacagaaatt ttgtcgtttt catcacgttt cgcattgatc
    17221 gctgcacgca tgaattccgc attcgtgata cctggtatct cacttggata ttgcatagct
    17281 aaaaataagc caagtcgcgc acgttcatct acttccaagt ctaaaacatt tttcccgtca
    17341 aacaaaattt cgccttctgt tacttcatag tttggattgc ccatgatagc ggcagataag
    17401 gtagattttc ctgtgccgtt aggtcccatg atggcatgga tttctccggt tttcattgtt
    17461 aggtttacgc ctttaaggat ttctttttcc tcgatcgaca catgtagatt ttttatttct
    17521 aagacagaca tgcattatac ccccaaattc tctttcgttc tcactcattt tagactaatt
    17581 gagaatgaag cgcaatgacc ttccttacta aaatcaaata tcttttattt attattaaac
    17641 aattttgatt tctaaattag aataggtata aaaaagatga gaaataagcc attacagact
    17701 tatttctcat cttttttgtt ccatacctta ttttgcatca gcctttactg gtttaacaga
    17761 gccgccccat ttgtctgtga tccattcttg gacttctttg cggtgaagca cttcaatcag
    17821 ttttttgatt ttgtcactgt cttcatcacc tttacgagtc gcaataatat tcacgtaagg
    17881 agagttgtcc ttttcaagca atacggcatc ttctaatgga ttcaaccctg cgccgtaagc
    17941 gaagtttgcg ttgattgcaa ctaggtcacc ttcatcattt tggtacgtag aagctaaaag
    18001 tgatgggtcg atcgtatggt tgaacttcaa gttcttaggg ttttcttcaa tatcatcgaa
    18061 tgttgctgtt tccagatcta ctccatcttt gactttgact aagccagcat cttgtagaat
    18121 tgtgatgatg cgtccccagt ctgattcaga atttgaagta atgattgttg caccatcttt
    18181 taattcggaa acatctttga cttttttaga gtacaagccc attggttcga tgtggacagc
    18241 accggcattg acaaagtcat atcctttttc tttgatttct ttgttcaaat aaggaatatg
    18301 ctggaagtaa ttggcatcaa tgtcgccttc ttccaaagat tggttcggta atacatagtc
    18361 atcgaatttg acgatttcaa gatcgattcc ttcatctttc aataatggtt tcacatgttc
    18421 caaaatctct gcgtgcggtg tcggtgaagc gccgactttc aatgtcgtgt cgtctgcttt
    18481 atttgctgaa tctttgctgt cgctgttgtt accgcatgct gctaatccta cagtcagcaa
    18541 aagcgctgct gcaaatccaa aaattttctt tttcatatcc tacatccccc tatatcctat
    18601 tatcttttgt ctgttttctt agttaagaaa tcaccgattc cttgaacgat aaatacgatt
    18661 agtaaaataa ttaaagtcgc aacgaatgtc actgttagat taccacgctg gtagccttcc
    18721 tgccaagcaa gacctccaag tccacctgcg ccgatcgcgc cggccatcgc agtaaatccg
    18781 atcatcgaga tcgttgtaac tgttaatcca gaaatcaatg cgggaatact ttcaggtatc
    18841 aagaccttcc agatgatttc ccagtaactt gctcccattg catctgctgc ttccagtaca
    18901 ccagggctga cttcacgaaa agcaatctct actagacggg cataaaatgg tgctgcggat
    18961 aaaacaagag ctggaatggc tgcttgcgca cctagcatag tgcctacgat tgctttagta
    19021 aatggaatga tcaaaaccat caggatcata aatggcgttg accggaaaat attactgatg
    19081 atcgatacga ttccataaat gattcgagca tacggtttgt ttttgcgtcc aatagagtaa
    19141 aggatcaatc ccaaaactaa tccgatgatc gtcactaaga ccattgagat taatgtcata
    19201 tatagtgtat caaggatagc ctgattcatc gtttccgggt tgatttgttt aaaatctaga
    19261 tatgtttctg aaaagctttt atccacgatg gatcacctca attcctactc cttgttcttt
    19321 gatgaacact tctgcttgat ctaattcttt ggtttcacca gtcatcaaga cagtcaatga
    19381 accaaaggaa ccttctttca cttgtttgat ctttccatac acaacgttga catcgatgtt
    19441 atagcgtcga atcgcttggg aaatgactgg ctcgttggca tttctttcgc taaaggtcaa
    19501 agttgccaaa cggccgcttg gattttcttt gatgagttca gctaatagtt ctgtcgaatc
    19561 agcttctggt tcgatgtctt gttgtacaaa gcgtttggtt acttcttttt tcggttgtct
    19621 gaagacagtc aatacgtctc cttcttctac gactttcccc aactccatca cagcgacttt
    19681 gttgcagatt ttgcggatca cattcatttc atgtgtgatc aagacgatgg tcaaattcaa
    19741 tttcttattg atcgctaaca ataagtctaa tacttcttct gttgtttgcg ggtccaatgc
    19801 gctagtagcc tcatcacaaa gcaggatctc aggatcattt gccaatgctc gtgcgatacc
    19861 gacgcgttgt ttctgtccgc cggaaagttg tgaaggatat gcttgtccgc gtccttctaa
    19921 cccgactaaa cgcaacagtt cttctgcccg ttctttacgt ttgctctttg gtacgccagc
    19981 taattccagc ggcagctgga tattttcaag tacagttctt gaccataaca ggttgaaatg
    20041 ctggaagatc ataccgattt tctttcggaa tgtacgtaat tctttgtttt ttaattttgt
    20101 aatatcttga tcgtttacga tcacttcgcc gctcgtggga agctcaagtc cattcaggag
    20161 acggactaat gtactttttc ctgcgccaga atagccgacg attccataaa tatccccttt
    20221 ttcaacgttt agtgaaacgt cattgacggc ttggatgttt ccgtttttcc cattgaatgt
    20281 ctttttgaca ttcgttagtt ggattaatga catgccattc acatctttct cttatgattt
    20341 ttcaaacaaa aaagccttcg ccctcttgcc ttaagcaaaa ggacgaaagc ttgaacttcc
    20401 gtgttaccac cttgatttac cgttacttcg caataacagc ctcttccagt acttggactt
    20461 agcttctttc cgtatactgt cgcgctgtaa cgggcactcc cgtagtaagt ttgcttacgc
    20521 tcgcttactc tgctctaaga ccatcttcca tctcattttc gttacccatt ttcagcaaac
    20581 agggctctct tcaaacgtcc gtgacatgta ctcttctttt caaagctttc tcgtttcata
    20641 taggattgct tatgattata gcaactggaa gaatcagtgt caactttttt caagtgattt
    20701 tctgatttta taattcgtca aattcttttg gatcaacatc tttgaaactt aagatccacg
    20761 cattcatttc atctgaatca ttcaagatat tgatatcttc accgatttta tcattaacag
    20821 aagaaactgt acccgttagc gggctggcaa attcattgac cgctttttcc gcttcaactt
    20881 cgatcagagg ttcatctttt tttagcgatt gtcctgcttt tggcagattg gcaaacgtga
    20941 tgtctccaag ttcttcttgc gcttcatttg tcaaaccaac acagtattct tttccattaa
    21001 ataagatcca aaggttgtcc tttttcttca aatgcttgcc tgccatttta tatcagttct
    21061 ccttcataaa ttttttcatt gaatccaatt gcaacaagct ggtcctctcg taagatcagt
    21121 ggtcgcttga tcagcatacc atctgtcgat aagacttggc tcgcttttga agccgtaaac
    21181 tcgcctactt gatctttcag tccaagttcc cgatatttca tcccgcttgt gttgaacagg
    21241 cgacggattg gtacttgact cgtttctagc caagtctcaa tcgttgaggc cttcggtact
    21301 tctttgatca tatcaacggt ttgataacta acgccatgct ggtctaacca cgctttggct
    21361 tttttgcatg ttgaacattt cgggtaccag taaaaggtgt acaattttct tacctccttg
    21421 aagatcttgc aggtgcttca ttttggtatc tcatcgataa tatcaacccg ataccgatca
    21481 tgttccctaa aatcgaggat cctccctgac taatgaaagg caatgggatc cctgttaaag
    21541 gaagtaatcc gatatttgct ccaatattct caaagacatg gaacagcagc atcatgatca
    21601 aaccgcttgc gatataggcg taaaattcat tattcgtatc gaaacacacc cgaatcatac
    21661 gatagattaa aataaaatac aagaaaatga cgaatgcacc accgataaag ccgaagtttt
    21721 caccgataac ggtaaaaatc atatcggact cacgaacagg cacatagata ttgcttacgt
    21781 tgaatccttt accaaacaag ccaccggtac caattgccaa aagtccttgg gccggctgat
    21841 aactcattcc tgacgtatca tggaaagggt ccagccatga atcaatacgt gcaaactgat
    21901 aagatttgaa tccaacttta taaagcaagt ctcttccgcc ttcagtcgta accaagaaga
    21961 tcgttcctgc tccaacgact actgccaaga ttactacagg tgcgatgatg cgccaagaga
    22021 ttccagacat caggaaaatc ccgccaaaga tcgcaagaaa gacaagcatc gttccaaagt
    22081 catcttgggc catgatcaat gctaaaaccg gtattgtcac gatcaagagc ttcccaatca
    22141 gccaaaagtc cgatttcatc gtccgttctt ttacttgcgt attatgttta gttacgatca
    22201 gtgccatcat caagatgtac gcaatcttca tcaactctgc tggctggaaa gtaaacgcac
    22261 cgaatcgaaa ccagttttta gaccctgttg aagcagctaa attctgatca tagaatttga
    22321 gcaagagtcc cattaccact aaccccaaac cataaaggta aggagtcagt ttccagatcc
    22381 atttggaatt caaatgcatg atgacaacga ttgctgccgc tccaactaaa taccagacaa
    22441 cttgcatccc tacgcctttc aagactgctg gattgttcgg atcatggcta agtgctacat
    22501 ataatgagag cagaccaatc aaacataaca gaaagactgg taaaatcacg ccataatcaa
    22561 tacgattatc gttcgaagct tttaattttt tttccatgtt tcatcccttt tcttaaaaac
    22621 catactgctg attattatag cggtccagtt agataatgga aagtctttcc atagacattt
    22681 gggaaaaatt aactttccgt ttgtgcagtt gcttgagcaa atgcagtaca aaagcctact
    22741 gcttatcttt agtgtatcat agaacttttt ttgatacatg caattatcta ccagctgaga
    22801 ttctcctcgc tgtgagaagt ttctgtgctt tcattgtatc gaaggatctc ttcggcaatc
    22861 tctagcagct gatcagaaac cagattggtt cgttggatga aataaaaata tcgatggaaa
    22921 ttcaacggag tcattggcac attttctgga acagagcgct tagacaaaat cgagcgaccg
    22981 attccttcct tcagcaacgc aacaattaca tcgttacttt tcacgatcat cttcttatct
    23041 tggatattca tctcttcaaa ataacgctgc gtgtaatgaa aaacaccaga gtttgcttct
    23101 cttacaagcc acaatttgct ttttgggtcc ccagcgatga ctagctggtc ttccatcaaa
    23161 gagtatcttt gtacgccgga agtctctaat ggcttttcga taaaaccaaa atcggattca
    23221 tgtttcgcta cttgttctaa tgattcatga gaattcgtga tttcgatctc aaaatgcact
    23281 ttaggaaacc ttttgatcag atgtttcatc aactgaggca aaagatagac agcaaaggtg
    23341 tgggaagcaa cgatccgaca tacttcttct tcattttgtt cattgagcaa tttttgctga
    23401 gtttcttccc attcttctaa caaaaccagt gtccgttcat ataataagtc ggcttgctta
    23461 gttggaagca tctcttttct tccgttacgt tgaaaaagat cgatattcaa ttcccgctcc
    23521 aactgtttga tttgattcga gactgctggt tgagataaaa acagtacttc tgctgctctg
    23581 gaaaaatttc ttgtttcata tactgctcga aaagtcgtta ataacttaaa catggcacca
    23641 cttccataac tttttatgat aattactata aaaactattt gatttaaata tacaaaaaac
    23701 catctataat agaaatgtac aacaaaacag ttgctgatcc aactagtaac ttagatgaag
    23761 acaatcagct gtctgcttgc tcgtaatcat acattcctat tcattttaca atgtattcgt
    23821 tcatcttggt actaaaatct attaaataaa aaggagaata ttatatatga agaacttttt
    23881 gaaaattatt ccgatcccga tctgcgggtt gatccttggg ttagcttccc ttggaaatct
    23941 gtttaaagac tatcaccatg ttgctttagg caatctaact ggcggtatct ctatgattct
    24001 tatgatattc atttttgtta aattgtttat cttattcgaa cacacgaaac aaacgttgca
    24061 tgatccaatc atcgcctccg tttcaccaac ttttacgatg tcactcatgg ttatttgtac
    24121 ttattttgtt tcatatgaaa gtattgcacc aattgtcaaa ttcatttggc tgtgcgctgt
    24181 tatcttccaa gtgatccttg tcttttattt caattatcac catgttatca aagctgatct
    24241 ttcaattgaa gcaatttatc caagctggtt tatcatttac gttggatttg gggtcatcac
    24301 tgtaactgcc gggaacttct tcccacttat tggaaaaatt ttcttttgga tttcattgat
    24361 ctgctatgcg ttcttattgc cgatcatcat caaacgtgtc tttttcgtaa gaaatatggc
    24421 tcatccgaca cgtccgctga ttacgatcgt tgcagcacct ggttctcttt gcctaacagg
    24481 ttatcttaaa aactttgctg aacctaattt ttatcttgtc gtagcattgt tcgctttatc
    24541 tcaagtattg tatttcgttg tgctgtctat gcttccacgt ttgttcaagc ttcgttttta
    24601 tccaagctat gcagccttta cattcccgct tgttatctct gcaacagcta tatttactac
    24661 tgttcattac ttcaacagtt taggtatcta tagtagcgta ttggatttct taaaagttgc
    24721 ggaatgtctg tttgcaagtg tagttgtctt ctacgttctt gctcattacg tacgtttctt
    24781 aattaaagaa catagcaaac atggaaaatt tgtttctaaa aaaatcgctt aataatcgat
    24841 tcaataggtt gtgaaaaagc tgttctgcat caagtaataa gaacggccaa acaaaaacag
    24901 ctgttcatgt ttttgtactg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    24961 ttaccaagag gtcgtaaaag aagtggtcag cttcaagaaa taagaaggaa tttacgaaaa
    25021 ttgcttctca aatttttgtg aaatttcggc ttatttccgt agaagctact tctggaacac
    25081 cgtttattcg aaacaagagg tcgtacaaga cttttgtcac gacctcttgt tcttttgtca
    25141 aaatactggt ttagtgctac tatgttcgag tatgctttta gtatttattg acgaaaggaa
    25201 ccgatcaaat gaaatcattg tcacatccaa taaaatctgt aaaggattca gcggtccgcg
    25261 aaatcttacc cggtttactg ctttcttttg ccatcgctgc cttcagcagg cttcttgcta
    25321 tttatctgcc acagttaggt ggagcgacta ttgctatttt attgggtatt ttattaggaa
    25381 atactttttt taaacagcct gttctagcaa tcggcacaaa atttgctgaa agcaaactat
    25441 tagaattctc tgttttttta ctaggtgcaa ctgtcacagc tcaaacgatt gctcaaattg
    25501 gtgtaaaagg aattgttttt gtgttactgc aaatgtcagt cactatcatc ggtgcttact
    25561 tgattggtaa aaaactgcaa ttcagcgata cttcttcttt gatgatggct ggaggaaacg
    25621 ctgtttgtgg ttcttctgct attgcttcaa tcgccccttc catccatgcg aaagaggaag
    25681 aaaaaggaca gatcatcaca ctagtcaatc tactgggtac tgtgatgatg ttgactctcc
    25741 cttttcttgg attacagttg tttggtggaa atctcattga aaaaagtgca cttttgggtg
    25801 gtacactcca atctgttggt caagtagtcg ctggggcaag cttacttgac aattctgttg
    25861 tcgaattcgc catgctattt aagattctac gtattatgat gctcgttgtt gttgtctatc
    25921 tgtttgaaaa attcatgcac aagaaacaac cagctctgtt tgaaacgcca actgatacgc
    25981 agaaaaaacg aaaattaccg cttccttggt atgtattagg gtttgtgatc ttttgtttgg
    26041 tcaacagctt gttttcttta cctgtagtta ttgaccaatc ggcacatttt atcagcagct
    26101 ggtttgagat cactgcttta gcggcaattg gtctgcgttt agactttgca aaattcatga
    26161 aagaaggtac acgcttctta tcttatggat tagctgtcgg gcttctccaa acagtgatgg
    26221 ctttactatt tatttggctg atcaaagttt agaatgaaca tgaaaaaaca gttgtgattc
    26281 tcgcatggaa caatgtccga atcacaactg tttttaattt tgcagatatc cttctaacaa
    26341 caacaattgg cgtttgattt caaccccatc tattgcgttt cccatatatc ctgtcagttt
    26401 tccattttgc ccgatcactc gatgacaagg aataacgatt ggcaatggat tggttcgatt
    26461 tgcctgccca atcgctcgca cagctttcgg actatcaaca gcaacggcta cttctttata
    26521 cgttcggatt tctccataag gaatggtcga caaagctttc cagacatttt gctgaaaagg
    26581 cgtacctctc tttatagata aaggaacatc aaattctttt ctttttcccg caaaatattc
    26641 ttcaaactgt tcttgtgtgc gcgttaaaat tcgctgattc gctgattcgc tttctgaaat
    26701 tggctgaaaa gatacagcag tcaatccgga ttcatctgca tctaaccata aaaagccgat
    26761 aggtgtgtgt attttcattt tgtttgctcc tagtcaatag actggaactt cctccttttt
    26821 ctctatccaa aactacttgc caactaataa aaaatgttct taaaagtttg ataaaccgag
    26881 gaaacatggt aaactaaata cgttagttta caaaaagaat acctgttctt ttacgtaaaa
    26941 tcaaatttta aaggtgttat ttaaggttta ttaaattttg aatgtaaacc atatataaac
    27001 atacgattga aaggatagaa acgatgaaag tatcaattgt tgtcccatgt tttaatgaag
    27061 aaaaaactgt tcctcttttt tttgatgagg ttgaaaaatt caaaggtgcc catgagttcg
    27121 aatatttgtt tattgacgac ggatcaaaag acggtacgtt agcagcaatc aagaagctag
    27181 cctcagctta tccttatgtc cgctttgtct cattttccag aaattttggg aaagaagcgg
    27241 ctctgtacgc tggattgcaa gcttctacag gagatttagt gaccgtgatg gatgttgatc
    27301 tgcaagatcc gcccgaactg cttcctgaga tgatcaaccg gatcactact tctgatatcg
    27361 attgtgtcgg cacccggcgt tcgactcgtg aaggcgagcc tgcgatacgc agtttctttg
    27421 cgaaaaaatt ctaccagttg atcaataaaa tcagcgatac agaaatggtc gatggcgctc
    27481 gtgatttccg cttgatgact cgtcaaatgg tggatgccgt cctagaatta acagaataca
    27541 accgattttc aaaaggatta tttagctggg tcggatttaa aacagaatat atttcttatg
    27601 aaaaccgcga acgtgtggct ggcgaaacat cttggtcctt ctggaaatta ttcaactact
    27661 cgattgatgg tatcgtgaac ttctcggatg caccgttaac gattgcttca tttatcggcg
    27721 ctttgtcttg tgtcggttct ggtattgcaa ttttgttcat tattttgcgg gcattgttgt
    27781 ttggtgatcc tacatcagga tggccttcca tggtctcgat cttcctgttc attggtggaa
    27841 tccaattgtt atgtcttgga atcatcggga aatatattgg gaaaatcttc ttagaaacga
    27901 aacagcgtcc tgtttatatc ataagagaaa cagaaaaagt gaagccaaat gaattgaagc
    27961 ctcaccaata atatacgtca aatgaagtaa acattttttt aaatgaaaaa ggactgtgac
    28021 aaaagtcttg tcacagtcct tttttccgaa taaatggtgt tccagaagta gcttcttcgg
    28081 aaataagccg aaatttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc
    28141 ttgaagctga ccacttctat cacaacctct ttcatatctt attacttctt cattgctgct
    28201 tttagtgctg ctgctaatgg gctttcttct ggttcttctg cttgtgcata ttttttcagc
    28261 aagcgtcgtt cttcatgttt ggtcattttc ttcttctgct cttttttatc aagcaatttt
    28321 tctgtcgttc catcgtattt acaacggaaa taagcaccgt ttttaccttc gatgatttcc
    28381 attttccggt gacattgcgg gcatcgatga ttagagactt ttggatcttt tcttctgcgg
    28441 tagttgcaat ctgaattggt gcagacatag attttgccgt cacgtgtgtt tttctcgcgc
    28501 agattggccc cacattctgg gcatttcttt tgggtaatcg agaaatcttg atattcttgt
    28561 ttgcttgatt tgatctcatt caccaagcgt tttgtttctt tttcgattcc attcaagaag
    28621 gttcctgctt gtttctttcc tgcagcaata gcttcaaggt ctttttccca gctttctgtc
    28681 aaatctggtg ttactaaaga gggatttacc aatactaata attgcttacc tttaggagat
    28741 acttgcagtc cttgaggggt acgttccatc agttcagatt tgattagttt ctcgataatc
    28801 tctgcacgcg tagctggggt tcccagactg tatttttcca tttgaccaag caagcttgct
    28861 tctgacaatg gtttaggcgg tgcagatagt tcttttttga tggcgaaatc gggattgatt
    28921 ctcattcctt tttcccattt gactgtttcc gtctcagttt cagtttcgcc tttccatcct
    28981 gcttggacga cacgattttg ccgaaaaatg aatcgttctt taccaaattc aaccgtaact
    29041 tttgtttggc tgactttatg gggttgagca aacagtccta aaaaacgaga gacaatcatt
    29101 tgatagattt ttcgctcttc attacttaat ttttcataac gtgctctgtt ttcagttggc
    29161 aaaagtgcat ggtgatcggt cactttactg tcttggaata cttttgtctg ttggacgatg
    29221 gctccgtttt tcaaatactg cttgacctca ggagcaaagt cgctgactgc ttgcaatcgt
    29281 tctttcatcg tacttttcat gtcatttgtc agatatttgc tgtctgtacg cggataagta
    29341 acgatcttat gggtctcata caaactttgt acaagactta atgttttctt tgcagaatag
    29401 ccatagcgtt gattggcttc tctttggatc tctgttaaat cataaggcaa tggcgcagat
    29461 tccgttttta atttttcttg gacttctgtg acagttcctt tgcttgtact gatcaattga
    29521 acaagttttt gtgcttcttc ttgtgttttt aaagcaaatt gatttttttg aagcattttg
    29581 gcttgttctt ttccagcttg caacgtgatc gtgaaatacg tttgtggacg gaactgctcg
    29641 attttctttt cttggtctct cactaatgct agtgtcggtg tctgcacacg gcctgctgat
    29701 aaattatccg cgtacttcac agttaaggct ctggtcacat tcaatcctac cagccagtcc
    29761 gcttttgccc gggcaagagc agattcatac aatgcatcat attctttcgc aggtcttagt
    29821 tgcttgaatc cagttttgat tgctttatct gtttgagaag agatccataa ccgcttcact
    29881 ggtttattga aatgcacata ctctaagatc cagcgagcaa caagttcccc ttctcttccg
    29941 gcatctgtcg caatgactgc ttctgagaca tcttttcgct gagccagctg cttgattgct
    30001 tttaattgat gtcctgtttt aggaagtggt ttgattccta gttttttagg gatcattggc
    30061 aacgtgtcca tttgccagct ctgccattcc ttattcagat cttccggcat tttcaatcct
    30121 aataaatgac caagggccca agtaacgatc accttgggtc cttcataata atttttgtgc
    30181 ttttgattag caccaagcac cttgcttagg tctttcgcaa cgcttggttt ttccgctaga
    30241 attagttgtt tcataaaatt cctttctgct ttctactgtg atgggctgat gatttttcaa
    30301 caacgcaagt cccttgtatt gctgtaaatc atcgtcacac tcttcacacc agcgctcatc
    30361 tccgtcattc caaaaggcgc tgagaaagtt actttttgca ttgatatatt cataattttg
    30421 atggatttcg ttccattgtt tttcgtacgc ttgtcgtgct tcttcgaaag actcgaatgt
    30481 ttcttcttct tcaatatctt cttcccagtc ttcaaaaaac caccaaggct cattgtcccc
    30541 gaacatggta attacttgat acatactcgt gctccttctt tcagcttttc tagactcatc
    30601 ttagtcactt ttcaagaaag ttgcaagaat tttctcttat aaaagggtca tttaatgaac
    30661 atcttaaaat tgttacaaat gaatctgatt gtttaaggca aagtagcgat aaagttattg
    30721 atcatccaaa aatagctgtc ttctggatcg attgttggtt cttttgtaaa gtatcctaaa
    30781 aaacgtaagg aaagataacc gtggatcata ctacgaagtt ccctgcttcg atgaactttt
    30841 tcttctttcg ttaattgaaa agcctcaaat atctgcaaaa tgatctgatt cgtttcatgg
    30901 attccatcta ataaaacagc ttcattggta tgcggtacac tgatcagtaa ctcatacact
    30961 gcttgatttg aaaaagcaaa ggatcgataa gtttctgcat agatctgcag tgcttcctga
    31021 ccactttttc ccacaagagc ccgtcgcagc tggtcattaa gttcctgcag aagtttcgct
    31081 gttagcgctg ttttgacatc acgaatattt ttgaaatggt tatacagcga gggagccttg
    31141 atatccaagc gtttggctaa atgctgaaag gttatttgct gaacatccat ctcttctgct
    31201 agttctcgaa agcagtcgat gattttctct tgagacagat ttcttttttt catttccatg
    31261 tttcctctca taaaataaat ccttcactct ttcagtttac cacgaaaaag aaagagaaaa
    31321 aagagacttt cattgataaa ctacatattg tagtttataa taaagagctg tagatgaaaa
    31381 ttttcagaaa gaaggagtct aattggctgc aagaataaag gaaaaattat ttaggaaaag
    31441 agaaacttgg gaacaaaact tgtttgtctt atggtttgga acatttatgg ctggtatcgg
    31501 atttagttta gtcatgcctt ttatgtcact ttacatagat acgttaggta attatagtac
    31561 gtcacaattg aacttttgga gcggattgac tttttcatct actttcttgg tgactacgct
    31621 tatctctcct tggtggggaa aattagccga ccaaaaagga agaaaattga tgctgttgcg
    31681 tgcttcatta ggaatggcaa tcgtcatttc tttgatgggt tgcgtgacta gtgtgtatca
    31741 attagtcgca ttgcgtctgc ttcaagggat tttttctggt tatatcagta atgccactgc
    31801 ccttgtcgca actgggacgc caaaagaaaa aagtggtcaa gttcttggaa ccttagcaac
    31861 tggttctgtt acaggaacat tacttggacc gcttcttggc gggatctctg cttcagcgtt
    31921 tggctatcgc cctacctttt tcattacagg gatcatctta tttttcgtct tcttgttgag
    31981 tctgtttttc gtccatgaaa aatttgtccc agtgaaaaaa gaaaacatgg tttctgccaa
    32041 acagattttc aaagacttga aatatccaca cgtcgtcatc ggtatgttcg tcacaacgat
    32101 gatcatccaa gcatccaaca attccattag tccaatcatc agtctgtaca ttcgccaatt
    32161 gctgcatgga catgggaatg ttacgttgat cagcggaatc attgcttcga tacctgggat
    32221 tgccactttg atagccgctc caagatttgg tcgcttaggt gacaagattg ggagtgaaag
    32281 aatacttgcg attggtttgg gttttgcgat tctggtttat atcccaatgg cattcgtcac
    32341 gaatgtttgg caattagctg ccttacgttt tttgattggt atctctgatg cttgtctgct
    32401 ccctgctgtc caagcattga tcacaaaata ttcgcctcat caagctgctg gacggatctt
    32461 cagttataac caatctttcc aagcaactgg taacgtagta gggcctatga tcggctccag
    32521 tgtttccagt gcatttggtt atcgtggcgt ctttctttcg acttctcttc ttgttttggt
    32581 caacttcttg cttgtacgac acaatacaca agagatccat cagcaagaaa ctgcagaaga
    32641 aaaagcttca ggagtctcca tagcttcagg gcactctcct tctcatagac aataagcgta
    32701 aatgaaaaac actgcttgaa cagttgtcac agactgtcct tgcagtgttt ttgtttaggc
    32761 ctgttttttc aagaactcag ataagaggaa taatcccttt tccaaggttt cttttttaca
    32821 acagtaacct agtcgggcat gcttcggtaa atcaaaggca cttcctggta ctaatagtac
    32881 tcctgtttct tctaataaat tgatacaaaa tgtctggtca tcttcttcga tggctaattt
    32941 aatgaaagaa gtagagacgt aattgggagc gacaagttca actttcggtt cattggctac
    33001 ccattgtttt aaaatcacaa ggttctctgt aatgatcttt ttgtttcgcg ccaatatttt
    33061 ttctttattc ttcaatgcat gtacagcaag atcatctgac aggactccgc cgcaaatcat
    33121 cgtgtagtct cgatactttc gaaaaacttc tgccaactca ggtccagtag ctgtccagcc
    33181 aatacgaata cctggaatgg agtaggtttt ggatagggaa ttggttgcta tacctttttc
    33241 atagacatct acgatcgaca agaattcacc tttatccgtt aaaggcgcat atacttcatc
    33301 tatcaataca tatgcatcta ctgtacgagc aatttctgcg attttttcta aagttttccg
    33361 atctagcaat gtgcctgtag gattgtttgc actattcaaa cagatgattt ttgtttcagg
    33421 tttgactaag gcttctagct gttctaaatc aaactgccaa tcttcttctt ccttcaaatg
    33481 aacaaagtca acagcggcac ctaaagattt ggggatgtca tataattgct gataagtagg
    33541 aagcatcgaa acaacatggt ctcctgcttc cactaacgcg tatagagcta aaagattagc
    33601 accagtcgct ccattcgttt gtaagatgtt ttccggatcg acggtttgat aaagagaagc
    33661 gacttcttgt ttgaaaacat ctgatccttc gatccagcca tagtccaatg gttcgttttt
    33721 gcgtttttcg aaaaatttac ttacgctagt cccatctaag ccaacgagtt cgtctaatgt
    33781 caatgcttcg atcgagcttt gtgcaatatc atataccgct ttcttttccc atttatttag
    33841 ccattcttct acaccgaaat ttgcgatttt catgtttctt tcccccttat atcttgatcc
    33901 agcctacagc aagcaacccg attgaaagca gtgcggccac acaaatcagc ccaatcatta
    33961 ctttttcttt tcccgtaatt tgatgtttgg tttcacgaca agcgatccag tagaagcaaa
    34021 aaccaggaag ataggcaata ctacataaaa ggacataaga aaatcctgct aaaatgatgc
    34081 ccatcaattc aaaaactaca gcaatcccac cgatcactac ttgttttttg attccttctt
    34141 ttaaagaaaa ctggacttga tagatcccga caagtagata acaaatcaaa attgccgctg
    34201 tacataaaga ataggcaaat tgataggcat aatcagtaac taagaaagtc aataagaata
    34261 gattagtcaa gattgcagta acaactaacg aataagttgg ggcatttttt ttattcgttt
    34321 ttccccaacc tttcggcaac agaccgtctc gagccatcaa catcgttgtt tctgctggga
    34381 gcatcgtcca agacaaccaa gaaccaatga tcgaaataat caagccgata ttgatcagcg
    34441 ctgctcccca atttcccacg attcctttta aaatatttgc catagcaggt tgtgaaatag
    34501 ttgctaactc ttcttgagat aagacgccgt atggcaagat cgaagcaagg atatagataa
    34561 acaataaaca aagcagacca atgatcgatg cttgctgggc ttctgaacgt ttcttcgcgc
    34621 ggttggctaa aacacttgcg ccttcgatcc ctacaaagac ccacatcata accatcaaac
    34681 aacctttaat ctgcaaccag atcgttcctt gctgccctgt tccattcact agattatcag
    34741 atacgttacc ccaaaaatca gcagtgaata tattcacttt aaatgcgata aatacaaata
    34801 ttaaaaatag gaataatggc actaatttac agatcgtcac gattgtattg atgacgctcg
    34861 ctccttcaac tccattgttt actaataaag tcagacccca aataaaaata cttgcaaata
    34921 aaatagaagg aacattttgt ccacctttga atatcgggaa aaattctccc atggcgctca
    34981 tcagcatcgt tgcaaaagca acatttccca gccacgcaga caaccagtaa ccccaaccac
    35041 tgatgaagcc acctaattta ccaaatgaag cttccgcgta accgaaaata ccaccatcta
    35101 gatctggacg cttttctcct aaatgattca gcgataatac caaagctaaa ataccaatcc
    35161 caacgattat ccatgaaagg atcgctggcc ctggagctgc agaactagcc agatcacttg
    35221 tgatcccaaa cacaccgctt ccgattgacg aactgacaac cagagcaacc agtccccatt
    35281 tgctaatacc cgtcgatttc ttttccattt ccatcaccct cacttataaa attgaacaaa
    35341 tcacttgcaa aagattccct tctattttgc aactgctctt gcttaacaaa aagcaacttt
    35401 attcatatta ttggatatta tgaacttttt acacaagtat ttcaagtata aaatgaataa
    35461 aaattaattt tattgcaaaa aaacagctat gctatccaaa agaaaaggtg tttcttttgt
    35521 tagccatagc tgttttgatt catttaatat cgcttaaacg tattgtaaaa cttttgtttc
    35581 ttgatatgcg tggtcgatca aaccaccacc cagacattcc attccatcat aaaatacaac
    35641 tgcttgtcca ggagtgattg cacgtacagg ttcgtcgaat acgacttctg cacgattatc
    35701 atccaataag cgaactgtca caggaacgtc ttgctgacga taacggaatt tcgctgtaca
    35761 cttgaattct tttggcattg gttcattcgt cgtaaagtgg atttcacttg catccaagct
    35821 tgtggcatac aatgcaggat gatggaaccc ttgccccaca tataatgtgt ttgtcgctag
    35881 atcttttcct acaacaaacc agggttcttg cgaatctcct ccgccaccga tccctagtcc
    35941 ttgacgttga ccaatcgtat aatacatcaa gccagcatgt tgtcctttga cttcaccatc
    36001 aagagtgacc atatttcctt tttttgctgg cagatagttg ctcaaaaatt gtttgaagtt
    36061 cttttcacca ataaagcaga ttcccgttga atcttttttc ttagcagtcg caaggcctgc
    36121 gcgttcagca attgcccgta cttctgattt ttccatgccg cctagaggga acatcgtttt
    36181 tgaaagctgt tcttgtgata attggctaag gaagtaagtt tgatctttat tattgtctac
    36241 tccacgaagc atgtggacca cgccgttttc atcgcgagtg acttgcgcat aatgtccagt
    36301 agctacataa tcggcgccaa gatccattgc ataatccaag aatgctttga atttgatttc
    36361 tttgttgcac atcacgtctg ggttaggtgt acgtcctgcg cggtattcgg ctaggaaata
    36421 ttcaaataca cgatcccaat attctttttc aaaattcaca gagtaataag gaatcccgat
    36481 ttgagcagct actttcgcta catccttata atcttccgtg gccgtgcata caccgttttc
    36541 gtccgtgtca tcccaatttt tcatgaatat tccgatcacg tcgtatcctt gttcctttaa
    36601 caaaagtgct gttacagatg agtcgacacc gccgctcatc cctacgacaa cgcgtgtttt
    36661 gctgttgtct gtcatgatat caccatcatt tcaattagat tctgaaacat ctacgagttg
    36721 aaggtcgcaa cttttcagta ttttacatta tacgtcatat ttctgaaaat ttcatccctt
    36781 aattatcaag tcgtaaaaat actgttctac tggacctatt cagtacacaa acatgatagt
    36841 caaccaaaaa atcactcatc ttttgagcat tcgatataaa aaattggcat actcataaaa
    36901 agggccagcc tttcggcaat aaggaataat tttataaatt gtgaaaaact atttttcaaa
    36961 attgcttctt atcgcttaaa gctgcacgtc tcatttacaa ccttttcttt ttagatgtta
    37021 tgagttgctc agataagtga ttttgtttac tctaagaaaa aagggaggca aaagatacat
    37081 gaattacttt atcagtgata tgcacttttt ccatgaacgc ttattaggaa aaaacgactt
    37141 tgctccgcgg ctctttgcaa atgtggatga aatgaaccaa cagttgatca ctagctggaa
    37201 tgcagtaata aaagaaaccg accatgttta tcatctaggc gaccttgcta tgcatccgca
    37261 atatgaaaaa gcaaatgcag aaattttgga actggtcact caactgcaag gaacaatcca
    37321 cttgatcaaa gggaaccatg acagtcgcgc ttttttccgt tatttagaaa aaaatgatcc
    37381 taggcttact gacggaagtc ccaaatttta tttttatgat gtaggtgcca tcgttaaatt
    37441 cgaccatcaa caatattact tgactcatta tccgatgttg ctcgggccta acgacactat
    37501 ccgcaatctt catggacata tccatcatta cagcgtacct atcgagaacg atgtcaatgt
    37561 aggcgtagac gcaccggaaa gagaactctt aaaggaaaaa ctaccatttg gcactccgtt
    37621 atctgagcaa caaatcaaag aaatctatga agcaaagagg aagtacgata ggtcgtgaaa
    37681 aaactgccct atatcaagta ttaaacaaat aaagataatt actcatattt ttcatttgag
    37741 taggcttatt gccacaggaa cattaggatc agaaatggag ttgtaacaag acttctgtca
    37801 caactccatt ccatactatt cgtctttttg ctcatggtgt tcttttacca gttttttctg
    37861 taattcttta tcataatttc gggcagctga cgttaaaaat aaaacgattc cgcataataa
    37921 aatcccgatt ccggaaaaca agaacatttt ttctacccct aaacgatcag ctaatggtcc
    37981 tgcaaataat aatccaattg gacctgtcag gttcatcaaa gagttgaagt tccctaagac
    38041 acgacctaaa acattcgatt cataactctg ctggatcatt gccatcagca gtgtattgaa
    38101 atatggcgtt gcacaacctg ctcctgcatt caagataaga aagtacaaga acccttgact
    38161 atcgtttgga accaatccgc ttgcaccgat cgtcaggccc ataatgatgt aggctataat
    38221 aataggtttc attcggtctt tccatgttcc tataaaactg atcagtgcac cgcctaagag
    38281 cattccgact gcgtagacta cttccactgc tcccgcctgt ccaacggaac caccaaagta
    38341 atctagcgtc atcaatggat acagactgat tgctggcatg aacaacagca tcacaaatgc
    38401 accattgatc gttatgtacc ataacccttt attatccatc aattgctgca gcccaaattt
    38461 tgcatcttta aaaatagtaa gcggaacttc caatatttca ggagaaactt ttgggatctt
    38521 cacaaaaatc aataagccga caccaaaaac tgcccccaat acatctaata aaataaggta
    38581 attgacagga acgactgaaa acaaagccgc acctagagct ggcgcaatga tgaaattcgc
    38641 tgattggacc attcctaact gtccattcgt ccttgttatg tgagaagatg gaacgattgt
    38701 cggcataatc gactggatcg ttggcatttg gaatgtttgt gcgaccgaac gcataaacaa
    38761 agacacaaaa actagccaaa tcgggaagga ttctgaaatc gttccgacta ctgctaaaat
    38821 aagcgcgaaa atcgctacaa taatatccgt gacaatcaaa agagcttttt tatcccagcg
    38881 atccactagc ggtcctacaa atgggcttag taaaaccatg ggaatcattc ctagtaaagt
    38941 agcaaaactc aaaattgtcg ccgaaccggt ttctcttgtc aaataccaga tgatcgcata
    39001 ttggacgacc atactagtga ttcctgacaa aaactgtccc gataaaaaga acaaataatt
    39061 tctcttccaa ttcgttaaaa cgaccgactg ttcattttcc ataaacatcc ctcttttctt
    39121 tttcattttg tatagcgttt gcacaacaat cattatacgg gattcttttt tcaaaaaata
    39181 cctttttccc taaataacaa tcgtttcaat ttaaatttct ttacttagaa acaataaatc
    39241 agcaaaatat taaaaataac aacatgtgtt aaatttaaaa ataaaaaaga caactaattt
    39301 gcgtcgtcct tacaaagtta gtcatctaac aataggccgt ttcaaaacta gttgtctttt
    39361 tatatccaca gatcattcga ctttttcgaa taacccacgg ctaatcatcc catccatcag
    39421 aaaataaaat ttatcttggc tcattttctg ttttggatct ttgaaaatat tgactgcttt
    39481 caatccattt gctgtagtga tcgacatttt caatgtttga atatcttttg atacagtgat
    39541 ttttaatttg aatccttctt tgctttgcgg cgtggcatct aatggtcgga tcaattgata
    39601 attgcctccg tttgtttctg taaatccatt atcgcgcaat gtatatcttt ttgcatcagg
    39661 atgcaaacga tagctaattg gtgcgcccag taccgttgct gttgtttcaa atgccatgat
    39721 tttcacctcg ataaaattag tttctcttta ttataaaagt ttatcctgat ttttactagg
    39781 tttttcagtt aagttcccta aaaaatgaag gcgttctctc agacatctgt tcatataaga
    39841 atgacttcac tagttatttt ttgacagcac gaactaattg atcaaggaaa tactggatgt
    39901 cgtcttcggt atttcccaaa ccaaagctga tacggatgga ttcttttagt atcggtgtat
    39961 cttctccata cattgcttcc aatacgtgtg aaggctcgat atttcctgct gtacaagctg
    40021 aaccagtaga aatagcgacc ccattaagat caagcttcat cagtagcaaa tcatttgctt
    40081 cgccaattaa gcgaagattc aacacatgag ggagtttatt cgttaaatca ccatttactt
    40141 gataagggat ttcgtttgct tctaatgtat caagaatcaa ttgctggaat cctttatatt
    40201 tgttgttacg tgcttcacgt tcttcaggtg acagcagttc aactgctttt gccatacccc
    40261 agatccctgc taaattttct gtacctgcac gccgcttttc ttcctgctcc ccgcctaaaa
    40321 gcaatgcagg gatatttact ccgtctcgtt tatataagaa accgacgcct tttggcccat
    40381 tgattttatg agcagagata cttaataaat cgattcctaa ttcttctgga tggatcgttt
    40441 ggtttccgta cgcttgtacc gcatctgtat ggaaataagc tggatgatcc gctaaaagtt
    40501 caccgatttc tttgattggc atcaaatttc cgatttcatt gttcccgtac atcacagaga
    40561 ccaagatcgt atcttctcgt aacgcttttt tgacctcttc gatcgacaga ttcccttttt
    40621 catctacagg cagatatgta acgtcaaacc cctttgtctc taaatatttc atggtattaa
    40681 gaacagcagg atgttcgata gctgtagtga ttagatgtct gccttctttt tgacgcgaga
    40741 acgctgtttc taagatagct gtattatccc cttctgttcc cccactgtta aagatgatct
    40801 cgtgaggttt tgcttgcaga ctgtctgcaa tgatttgtct tgctgtttcc aatttttcat
    40861 gcgcgtggcg accaaatcca tgtatgcttg aaggatttcc aaacgtgtct tgcatgattg
    40921 ccgtcatcgc ttcaattact tgtggatgca tgggggtagt tgccgcatga tccagataaa
    40981 tagttttcac ttcattcatc ccttccaaat tattgctagg cacattctag cataaaatag
    41041 cgaacaaacc aaagaagagc tcatttttgt ctagaggacg tttcatgggc atagaaaaaa
    41101 ggagctgtga caaaagtctt gtcgcagctc cttgaagctg ctcacttctg tcacaactt
//
LOCUS       DAYYFU010000020        40414 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000020 DAYYFU010000000
VERSION     DAYYFU010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.020"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="ACHA34_001251"
     CDS             complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="ACHA34_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFM8791906.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="ACHA34_001252"
     CDS             complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="ACHA34_001252"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFM8791907.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2303..2965)
                     /locus_tag="ACHA34_001253"
     CDS             complement(2303..2965)
                     /locus_tag="ACHA34_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8791908.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3149..4795
                     /locus_tag="ACHA34_001254"
     CDS             3149..4795
                     /locus_tag="ACHA34_001254"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8791909.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4786..5868
                     /gene="uxuA"
                     /locus_tag="ACHA34_001255"
     CDS             4786..5868
                     /gene="uxuA"
                     /locus_tag="ACHA34_001255"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8791910.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5976..7529)
                     /locus_tag="ACHA34_001256"
                     /pseudo
     CDS             complement(5976..7529)
                     /locus_tag="ACHA34_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7677..8818
                     /locus_tag="ACHA34_001257"
                     /pseudo
     CDS             7677..8818
                     /locus_tag="ACHA34_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8944..10023)
                     /locus_tag="ACHA34_001258"
     CDS             complement(8944..10023)
                     /locus_tag="ACHA34_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8791911.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10126..10566)
                     /locus_tag="ACHA34_001259"
     CDS             complement(10126..10566)
                     /locus_tag="ACHA34_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8791912.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10623..11033)
                     /locus_tag="ACHA34_001260"
     CDS             complement(10623..11033)
                     /locus_tag="ACHA34_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8791913.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11213..11647)
                     /locus_tag="ACHA34_001261"
     CDS             complement(11213..11647)
                     /locus_tag="ACHA34_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8791914.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11747..12595)
                     /locus_tag="ACHA34_001262"
     CDS             complement(11747..12595)
                     /locus_tag="ACHA34_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8791915.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            12984..14750
                     /locus_tag="ACHA34_001263"
     CDS             12984..14750
                     /locus_tag="ACHA34_001263"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFM8791916.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            14852..16111
                     /locus_tag="ACHA34_001264"
     CDS             14852..16111
                     /locus_tag="ACHA34_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8791917.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            16258..17559
                     /locus_tag="ACHA34_001265"
     CDS             16258..17559
                     /locus_tag="ACHA34_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8791918.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            17562..18413
                     /locus_tag="ACHA34_001266"
     CDS             17562..18413
                     /locus_tag="ACHA34_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM8791919.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            18426..19241
                     /locus_tag="ACHA34_001267"
     CDS             18426..19241
                     /locus_tag="ACHA34_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFM8791920.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            19392..20558
                     /locus_tag="ACHA34_001268"
     CDS             19392..20558
                     /locus_tag="ACHA34_001268"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8791921.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            20788..21435
                     /locus_tag="ACHA34_001269"
     CDS             20788..21435
                     /locus_tag="ACHA34_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8791922.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            21519..22463
                     /locus_tag="ACHA34_001270"
     CDS             21519..22463
                     /locus_tag="ACHA34_001270"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFM8791923.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            22598..24049
                     /locus_tag="ACHA34_001271"
     CDS             22598..24049
                     /locus_tag="ACHA34_001271"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFM8791924.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            24070..24906
                     /locus_tag="ACHA34_001272"
     CDS             24070..24906
                     /locus_tag="ACHA34_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8791925.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            24906..25565
                     /locus_tag="ACHA34_001273"
     CDS             24906..25565
                     /locus_tag="ACHA34_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8791926.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            25691..26380
                     /locus_tag="ACHA34_001274"
     CDS             25691..26380
                     /locus_tag="ACHA34_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791927.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            26386..27342
                     /locus_tag="ACHA34_001275"
     CDS             26386..27342
                     /locus_tag="ACHA34_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFM8791928.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            27386..27898
                     /locus_tag="ACHA34_001276"
     CDS             27386..27898
                     /locus_tag="ACHA34_001276"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8791929.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(28154..28702)
                     /locus_tag="ACHA34_001277"
     CDS             complement(28154..28702)
                     /locus_tag="ACHA34_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8791930.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(28791..29972)
                     /locus_tag="ACHA34_001278"
     CDS             complement(28791..29972)
                     /locus_tag="ACHA34_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFM8791931.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(30208..31716)
                     /locus_tag="ACHA34_001279"
     CDS             complement(30208..31716)
                     /locus_tag="ACHA34_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFM8791932.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            31875..32066
                     /locus_tag="ACHA34_001280"
     CDS             31875..32066
                     /locus_tag="ACHA34_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791933.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            32269..33186
                     /locus_tag="ACHA34_001281"
     CDS             32269..33186
                     /locus_tag="ACHA34_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFM8791934.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            33179..34252
                     /locus_tag="ACHA34_001282"
     CDS             33179..34252
                     /locus_tag="ACHA34_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791935.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            34400..34918
                     /locus_tag="ACHA34_001283"
     CDS             34400..34918
                     /locus_tag="ACHA34_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791936.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            34899..35291
                     /locus_tag="ACHA34_001284"
     CDS             34899..35291
                     /locus_tag="ACHA34_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFM8791937.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            35367..36302
                     /locus_tag="ACHA34_001285"
     CDS             35367..36302
                     /locus_tag="ACHA34_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8791938.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            36435..37214
                     /locus_tag="ACHA34_001286"
     CDS             36435..37214
                     /locus_tag="ACHA34_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8791939.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            37207..37524
                     /locus_tag="ACHA34_001287"
     CDS             37207..37524
                     /locus_tag="ACHA34_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8791940.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            37768..39378
                     /locus_tag="ACHA34_001288"
     CDS             37768..39378
                     /locus_tag="ACHA34_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8791941.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            39535..40383
                     /locus_tag="ACHA34_001289"
     CDS             39535..40383
                     /locus_tag="ACHA34_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8791942.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        13231 a         7260 c         7799 g        12124 t
ORIGIN      
        1 aaaattcttg tcaccatcct tttttccgct ctctgacttt ttacaatctt catagctcaa
       61 gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca
      121 atgatttgtt tgtatggact atctgtacaa tcaccagctg cgaaaacgcc ggggatattc
      181 gttgcgccat ttttctcggt cactatttca tttcttggat tcatttcgat cgttccatct
      241 agccaatccg tattagggag taagccgact agaatgaata ctcctgaaac agctagtgta
      301 tgcatctcca tcgtacggcg atcgatatag gagagagatt ctacctgatt cgtaccgctg
      361 atttctttcg tcgctgcatg tttgataaca gtgacattcg caagagagac tagtttatcc
      421 tgtaagattt gatctgcttt taattctggt aagaattcta gaacatatac atgttttgct
      481 aacccagcca aatcgattgc tgcttcaaca ccagaattac caccaccgat aacagcaatc
      541 tcttttcctt taaataaagg gccgtcacag tgtgggcaat aagcaattcc tttattttta
      601 aactcttttt cgcctggtac attgatagaa cgccagtgcg cacctgtcgc gatgatggcg
      661 gtttttgctt taagtgatat accgttagct aagccaatat taataaaatg ttgctcttta
      721 tttaaggaaa cggcctgttg attcatcata atgtcgatag ggtaactacg cacatgactt
      781 tctacttgct gcatcaattt tggaccttct gtgtaagagg taccaatcat attttcaatc
      841 cctagtgttt caacgacttg tccgccgaaa gaatcagcga caagtcctgt acggattcct
      901 ttacgtgctg catagatagc tgcactacta gctgctggtc caccgccaat cactaataca
      961 tcataaggtt ctttttcaaa caactcttct tcaatcaaag ggccagtgat tttttcaaca
     1021 atttgttcga tcgtcatgcg tccgctacta aattcttctc catttaaaaa cacagttggc
     1081 actgccatga tttttttatc atcgatttct ttttggaaca tcccaccttc aatcatcgta
     1141 tgagtaatat ttggatttaa tacagctaat atattcaatg cctgtacaac gtcggggcaa
     1201 ttatgacaag tcaagctaac ataactgtca aaatgcaatg tttgtgttat ttttttgatt
     1261 ttaagactca aagattcact gattttcggt gcccgtccac taatttgtaa catagccagt
     1321 aaaaaagagg aaaattcatg accgaggggg ataccagcaa aaacgattcc actttctttg
     1381 ccaactggat tgatagaaaa actaggtgtg cgcatcgaat ctcttttttc ccaagagatt
     1441 ttaggagaca tttctgctat ttccttcgta aattcagcta gtttttgact attttcacta
     1501 ttatccaaac taacagaaaa aacaatagga gattcaatca gtgctaaata ttgttcgaga
     1561 ttttgtttag tttctttgtc taacatatcc accacctaaa tctttccaac aagatcaaaa
     1621 cttggtttca atgtttcgcc actttctttc cattttgccg gacaaacttc acctgggtgg
     1681 gtacgtacat attgagcagc gcgaatctta tcaatcagtg cactagcgtc acgaccgatt
     1741 ccatctgcat tgatttccat agcttgaacg attccgtctg gatcaataat aaatgttcct
     1801 cgctgagcta atccttgctc ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac
     1861 ggatcaccga tcatcacgta ttcgatttta ccgatagcat ctgatgtatc atgccatgct
     1921 ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt caacacctaa agatttcaat
     1981 gtactgtatt gttcttgtaa gtcttctaat tcagtaggac aaacaaaagt aaaatcagct
     2041 ggatagaagc aaagaatact ccagttcccc ataagatctt tttcactgac ttctaaaaat
     2101 tctccttgat gataagcatg tgcattaaat tcttcgattt ttttcccaat caatgacata
     2161 gtaaaacctc caataataat tatttattta taattattgt aatttagttg gagttgcaaa
     2221 gtcaatggat attttgttat ttaataaaga ttactcaaaa acgaaggtta attataaaaa
     2281 tagaagaggt cgcaagaaaa tgctactgct ggaaatactc gggataacgt tgagtaacag
     2341 tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg
     2401 gttttttatc aactaaagcc ttgagtaaat cataatgttg ttccatgatc gtttcccatg
     2461 gaagcccctg aatggtcact cgtaaccagc ggaaacgttc taaatgggta ttatggtcat
     2521 ctaaccagtt ccaaacttct tttcttcctg caatttcgaa acaagcttca tgaaataaat
     2581 tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac tgctttttct tgttcttcta
     2641 aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga
     2701 tttgctcttc caactgttct cggacaaagc gagcattttc tgcagaaaca agattaattt
     2761 tggaaacata cgtaccactt tgaggtatcg tatacacgag ttcttgctga cgtagttgaa
     2821 tcaatgcttc gcgaatggga gtccggccga tagcaagatg ttcctccagt gccttttcag
     2881 atattttcct tccaggttct aagtcagcat agataatttg tttacgaatc gcttgataag
     2941 cttgtgattg caagctgctt ttcatcttta ttcctccaaa atcatttcta tgaacgtagt
     3001 ataacagaag aggtctgaaa aacaatctgg tatgtcacat taactagtaa atttgtataa
     3061 atagtaaaat tttggttgac atagcgattt ttattgcata aaattcactt gaatacaaaa
     3121 actaatatgt tagtttagga ggggaaaaat gattgattta aacaactgga ggaactttat
     3181 ccctgaatta tctcaaaaag aagtagaaat acctcatttt gaccgtgcat cattaagaga
     3241 aaatggacaa aaaaatcctg tctggatcca tttcggtggc ggaaatcttt atcgaggatt
     3301 tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt
     3361 ttgtgaaaca ttcgatgaag aaataattga aagggcttac caagattttg atgatttgat
     3421 tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc
     3481 tgacagtctg tattgtcatc cagattgttc tgaacacttt gataagatga ttcgacactt
     3541 tgaaaatcct agtttacaat tagttacttt tgctattact gaaaaaggtt atcgattgaa
     3601 agatacaaat aataaatata taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa
     3661 gccatcacat acaatgagta ttcttgctgt gctactttat aaacgttttt tatctggagc
     3721 ttggccgatt gcacttgtct ctacagataa tttttcacaa aacggtagtc aattacgtac
     3781 ttctttgcta acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa atacatttat
     3841 ccaatactta tcgaatgaag aaattgttgc gtttccatgg acgatgattg atcgtatcac
     3901 cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa gagggctttt caaatatgtc
     3961 gattttccaa accgcaaaag gaaataatat tgctccattt gtcaatacag aggacatcca
     4021 ttatttaatc atggaagatc attttcccaa tggacgtccg aaattcgaaa aagctggggt
     4081 attcttgact gatcgtgcaa cggtagataa agcagatacg atgaaggtga cgacttgcct
     4141 taatcctttg catacgtctt tagctattat gggttgttta ctggggtata ggagcatagc
     4201 tgaggagatg aaagatccag atttatcgct gctagtttct gaaatcgggt acaatgaagg
     4261 cctcccagtt gtggagtctc caggtatcat tgatccaaag atctttctgg aagaagtaat
     4321 tactgtgcgc ttttctaatc caaatattcc taatatgcct cagcgaatcg ccacggatac
     4381 ctctcaaaag gtggcgattc gttatggtga gacgattaaa aaatatatgg aaatagaagt
     4441 taaagacccg cagacacttg tattcatacc attgactctc gctgcttggc ttcgatacct
     4501 tattggcatt gatgatcaag gaaaaccatt catcccgagt ccagacccac tattgacaga
     4561 ccttcagcat aagctttccg atattcaact gggtacgcaa gatacatcac atattcatga
     4621 gaaaatggct cctattttgt caaataaaga aatttttggt gtagatcttt accaagcaga
     4681 gcttggcaga aaaatagaag aaatgttcat caagatggta gcaggaccaa atgccgtacg
     4741 aaaaacatta caggattact tacaagaaca tggaggaaat ttatcatgga aatgactttt
     4801 agatggtacg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
     4861 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcctagggag
     4921 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
     4981 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
     5041 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
     5101 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
     5161 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
     5221 atcgaacatt ccagcaatga atttggattg ccgggatggg aacctgagcg cttagctcct
     5281 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
     5341 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
     5401 catccagatg atccgccata ctcgattttt ggattagctc gaatcgtaaa gaatcgagaa
     5461 gatttagatt ggttgtgtcg catagtagac agcccttata acggaatcac tctttgtaca
     5521 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgccaa
     5581 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
     5641 tatgaggcac cacataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
     5701 atgtatgaca acggatttaa cggatacatc cgtcctgatc atggaagaat gatctggaat
     5761 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
     5821 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
     5881 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
     5941 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
     6001 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
     6061 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
     6121 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
     6181 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
     6241 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
     6301 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
     6361 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
     6421 gtttggactt ctttcaatac gctatgttct tgataaagca tgaaagtagg aaggggcgta
     6481 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
     6541 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaag ggactccacc
     6601 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
     6661 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
     6721 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gagatcaata
     6781 aaaaagtcaa tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca
     6841 ggaaaattct ctttacatat ttttaataga aaatataatt tatctatcat accgctattt
     6901 agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga
     6961 tcttcatcat aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct
     7021 gcatattgaa tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag
     7081 atagacaaac cgtactatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa
     7141 gaggcactat agtttgtttg ctgactgaaa ctgataatcg aatttgtctg acagctgaac
     7201 aaagaagtca agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa
     7261 tagcagtaag ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca
     7321 atatgcagtt ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt
     7381 aaaaaatttg attcagacat atggaaatga tctgctaaat attgtgtagt aataaagaaa
     7441 cgatgttcag ataggaaaag aaaaatatct aattttgtat tgcttttttt cttcaagata
     7501 aacgttttaa tcgcttcttg agtttgcatg aaagaggctc ctttttactt aaattgttaa
     7561 aacttgaaaa tattactgtt tttttcataa atttagacaa aaaaacagta aacatatagg
     7621 tagaatctct actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga
     7681 gatattcaac catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc
     7741 agacagtcca cacagaggag acagaaacag ccaaatggac agaaaccaag tgggccaacg
     7801 tcaatcgaat tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa
     7861 gaaaaggaca aacagcgacc atcaaagcaa aaattgacta ttcgggagac ttagcaccta
     7921 tactacaatc agctgttaca tttgagaatg ttgatcccac tttgtcgatt ggctatgacg
     7981 agaacacttt gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc
     8041 aggacttgtt aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc
     8101 atcatttaac gccgtatagt cagcggcaga caattgagca agattcagcc atcgactcag
     8161 gaggagatct tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac
     8221 cgcctacaga aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg
     8281 atacagaaac tcaaccagag ccagacaata aatcaaaacc tgaaataacc cctggagaaa
     8341 aaccagatgt aactccagaa ccagatacgg attctggaaa ccaaacagta ccggaaacaa
     8401 atccagacac agataatgaa actgaaaatc cagaaaaacc agaggtagat ccagaagaaa
     8461 aaccagatgt gactccagaa ccagatacgg acgcaagaga ccaaggaata ccagaaaaaa
     8521 taaacaaaaa aacaattcaa gaggatggaa aaaaagagag taaaaaatca aatctagcaa
     8581 ttctaaaaat aaatgaagag cagctgaata aaaaatcacg aatatttgat agcgcccaat
     8641 cagctgaaac cttaaaaagt agcaaggata caacctttgc tgtcctgaaa cgaaaaataa
     8701 acagctacca aaatccggag agtcacaaaa caaagttata ctgtggagcg gaatcattct
     8761 attaagtata gcaacaatgc tttctgcaaa aagatttaaa caaaacagat ctctctaaca
     8821 tttgaataat gtctgtgaac gcatttttca aataaaaaga aatatccaga aaatgacccc
     8881 aaaataccag attgaaatct gatatttggg gtcgttcata ctttatcttt cttcatattt
     8941 attttaaggg atatctgttt cttttggaag atggttcggg atctttttca ctttcaatac
     9001 atctaaaaag aaggtaaaga tcggaatgcc gacaatcaag ccccaaacgc caaagaaccg
     9061 ttcgctaaca agtaagacca caaatgtata aaaaatcggc agttctgttt tgctggacat
     9121 caacttcgga ttcagcacat aagactcaat tagatggaca atcaaaatcg taatcaggat
     9181 ataaacaaca tctcggatgc ctccttggga ataagctatg aaagatagag gaatacaaga
     9241 aatgataaca cctgctactg ggatcagact taaaatgaaa atcatgatcg ctaaactcag
     9301 cagctgatga aagccaaata cagccaaggc gattgtagtg agcaacgtat ttaaaagagc
     9361 gacaacaaat tgagcttcta ataccaaacc aaatgtattg acaaacttat cggcaaaata
     9421 gtaaatatct tgaaagaacc aagaaaattc actttttaaa aacaatcttg agaatacaat
     9481 tgtttttttc ttttcaatca taaaaaagaa acttaagatg aatgacatag caaacgccac
     9541 tgccattttt cctatatcct gtaaatagcc aagcgcaatc gaagcgccat tttgaagctg
     9601 ttcaaaaaaa tcgtttcgct caatataagt atggatatat tgtaaaacag gatctgcatc
     9661 ttttggctga tgctcgtaaa aattaataac ggactggatc atgtcaaatg tttgatgaat
     9721 aagcatcggg atatactttg tgaatgccag atagataagg taaacgatca gtgtataaat
     9781 catgatagct aaaacacctg tagggattcg gacaaatcgc tgaatgattt gaacaagatg
     9841 aatcatcaaa taagtaaaga taaatgtgag caaaatggtt gtaatcatac ttctaactaa
     9901 gaaaagaaca aaaacgacaa ataataaaac agtgagacgg cgtatttttt cattcgccaa
     9961 aaaacgctga taaacggtca aatcgacaac ctccaaataa caaaaaataa acacgttatt
    10021 cattatatga tggaatatga aaataagaaa gagcaaaaag aaaaaccgct aaatgacgtt
    10081 atcttatgag ttggttttcc tcataaaatc gttcatctag cagtttcagc cctttttctg
    10141 gtcttgatga aaattatcta gcatttttcg ggtcagatgt tgcagtgtat ccaattcttc
    10201 ctgttcaagt ggaagataag acttgatctt ttgtggaata ccttctgctt gtttttgcaa
    10261 cgcagcacct tgagcagtca gcgtaacatt tacgattctt tcatcacccg tttcacgctg
    10321 acgattaata taattttttt ctgctaactt ttttagtaag ggagtcagcg taccagaatc
    10381 cagacacaaa tcatttccta gctctttcac tgaagtcttt ttcttttccc ataaaactaa
    10441 catcacaaga tactgggtat aggtaagatt cagcggcttg aggaggggag tgtagtgtct
    10501 aatgatttct ttagaggcgg catataaagg aaaacatact tgattaacta aatatagatc
    10561 ttccattttt ccacctccgc tgttttacta tagtgtatca tagcggattt acacaaaaaa
    10621 gtctattcac taactgcttt cagagtaact tctatatttc cagctgtagc ttttgaatac
    10681 ggacacactt tatgcgctgc ctctaataat tcttgcgttt cttccagaga aagaccttcg
    10741 atagatcctt caatctctac acctaagaca aagctcgttt cactttctga ataaagggaa
    10801 acttgtgcac tgacagtaga agccccagag atattttttt gctgtttgat caattctaat
    10861 gcactgttaa aacatgcact gaaaccagct gcaaacaatt gttcagggtt ggttgcattt
    10921 tcgacgcgtt taccaggagc tgcaatattc aattcgaatg tacgatcggg attatgcact
    10981 tctccagagc gtccgcctgt gttgatcatt ttcgtagaat acattttttt cataaaatta
    11041 ccacctttaa ttatatttta tacaattgaa ttttacacaa tataattgtg gaaagcaaag
    11101 aaatggattg gcataagata aataagagaa agcacatgaa acagacgcgc tttctcgaat
    11161 agcaaacata tacaaaaaat atcagagaca aaaaataatc tcatcataaa cattacatat
    11221 atttctcaaa aagttgtttt ctttcttcaa aaattacttc acgatggacc tgatgaagca
    11281 tccataaata accaaaagga tctctgaaca ttgagttagt cacaccaaag tccatcattt
    11341 ctgtcatttc ttgaatctct acacagccat ttcgtaatgc tttattatgt gtgtcctcga
    11401 tatcagagac gatgatattg aaccagatcg tatttggatg ttcagcagta ggagcagtca
    11461 aaccaagaga cgtgttttca tccagcatat ggaatctcgt accatacaag gtaaaaatga
    11521 cttcattatt gcctctaggg taatctgttg cctcaatcag ttgtatttca tcaaagattg
    11581 tttgatacag ttctagtgct ttcaaactat catttaatac aaaatcgatt tccgttcctt
    11641 tatgcataaa gatctcctct ccttttcagt cgaacagtta ctagattctt ttattatacc
    11701 agctaaagaa aacgcattca aaaaaatagc ggatttactt accaactcat tcgattggat
    11761 gataagtaaa tccgcgtccc attacttctg ctacgctgct gacaatgaca aaggcacttg
    11821 gatcgatttc ttctatcaat ggtttcattc gggaaaattc atgatcagtc acaacgatca
    11881 ataaaacctc ttttgacttt cgattatacc cgccttttgc ctctagcagg gtaaggccac
    11941 gtccaatctc agcacttaag cgaaggcgga tgtcttccaa ataatgttca ctcatgacca
    12001 taacagattt ctttcgattg atacctgttt cgatataagt catgacaatt gaagtgattg
    12061 caatagataa aatagcataa agaaactctt ctattccaaa aataaacaga ttaagagaaa
    12121 caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt caagtggaaa tattttttaa
    12181 aaatgagggg agggatcgtc gtaccgccgc tagaagagtt gtttttatat aaaatcgcaa
    12241 ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa caaccgatct gaagtaatca
    12301 ttgtttctgg aactattgca atgattaaag gaaaaaccaa gcttccgaaa agtactttaa
    12361 aaaagggttt tttacctaaa aatagaagag ctagtaccaa cataacgata ttcaacacta
    12421 aaataactat agctcggtca aagcctaaag cagcttccag taatattcct agaccgctga
    12481 cacctccagc agcaatatga tgaggcccta aaaacatatt gatagcgact cctaatatga
    12541 agagggcaaa aataatctta atgtatgtcc atataaattg tctcgtttct ttcatttatt
    12601 cacttcctta attcattaat aatactctta tcttattata acttactttt caccttaagt
    12661 taactttaat taataaaaaa attaggctag tctaaattat tatgtttcat tcttctagaa
    12721 tatacttcta cgccaaaaac tgttaaaaaa cttttacttt agaaaattgg agaaattgat
    12781 cgcttaagat aaattcagga cactaaatag tacaggaagt agactaaaac ttttaaaagt
    12841 tttaagtcta tctatttggc aagtttaaaa aataaaaatt cggtatcaaa aaactgttac
    12901 cgataacatt tttgaaagcg ttgtcttttg cttctctatc acttatagtg atgacgtcat
    12961 attaaaacac aaggaggaaa tcaatggata cagctgcaat ttatcatcga ccggaaagtg
    13021 aatatgcatt tctatatacg aatacaagat ttcgtattcg gcttcgtact aggaaaaatg
    13081 atatcaaaaa agtatatgtg ctatgtggag atccatatac cataacaact gaaaaatggt
    13141 atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa
    13201 ttgaagtgac gagcgaaaca agtcgcttac agtatgcgtt ccatgttgtt ggggaagata
    13261 acacggactg tttttacggt gatcaaggaa tttttcctta tcgagaaaat gtgattactg
    13321 aaccaaattt ttattttcga ataccttatt ttcatcaaat cgatcgattc actattccag
    13381 aatgggtaaa agaaacagta tggtatcaaa tttttcccga aagatttgca aacggggaca
    13441 aagcaaatga ccccgaacac actttacctt ggggaagtaa agatccagac cgagaagact
    13501 ttttcggggg agatttgcaa ggtgtgatgg atcatttgga ttacttgacg gaccttggca
    13561 tcaatggcat ctatttttgt cccatattta aagccacatc caatcataaa tacgatacga
    13621 ttgattatta tgaggtggat cccgcttttg gcgataaaaa acttttaaaa aacttgattg
    13681 atgaagcaca taaaagaggg atacgtatca tgttcgatgc tgtattcaat catatgggtg
    13741 tccattcacc acagtggcaa gacgttctca aaaacggaga aaaatcgatc tataaagact
    13801 ggttccacat ccgttttttc ccagtagatt cttatcagat gactgactta ccagaaactg
    13861 ctgaaaatat cccatatgat acttttgctt ttactccatt catgccgaaa ctaaatacag
    13921 caaatccaga agtgcaaaaa tatttgctgg atatcgcaac ttattggata aaagaattcg
    13981 atattgatgg atggcgtctt gatgttgcta atgaagtcga tcatcatttt tggaaaaaat
    14041 tcagagaagc agttacagaa atcaaaccag acatctatat ccttggagag atttggcatt
    14101 cctctcaagc ttggctgcaa ggggatgaat tccatgcagt gatgaattat gcatttacag
    14161 attcgattaa agattatttt gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa
    14221 tgaatcatca gcaaatgctt tacagagatc aggtgaacga aggaacattc aacttattag
    14281 attctcatga tactgccagg atcttgaccc tttgccaagg taacaaagaa ttgatgaagt
    14341 ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg tatctattac gggacagaaa
    14401 tagggatgac tggtgaagat gacccggatt gccgcaaatg tatgatatgg gagaaggagg
    14461 aacaagatct agagctattc ggttttataa aagaacttgt ttctctaagg aaacagttgt
    14521 ctaagattat ttctgaaggt tctactcagt ggctaatagt aaatgatcgt gaagataagc
    14581 tctatttcac acggaaacta gagggacaga tcatctatgt ttactttaat cagtcaaaag
    14641 aaccttgggt agtagaacag gaaaatgagg tgattcttag tcaaaattgt cagttgttag
    14701 aagacgggaa agcagagata cgacaatacg gatttttgat catgtgttga aaactgttac
    14761 cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc ccattataat aagggtgtaa
    14821 cagcacgttg aatacaaagg aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg
    14881 tttagggtta atctctttag gaatcttagg cggattagcg gcttgtggca atggtaactc
    14941 ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca gtttccgttg attctggata
    15001 taaagattat ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa
    15061 gctagtagaa aaggacatgt tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg
    15121 aaagggacct gacgttatga tggcagcata tgaccggatc ggtgcgttag gacagcaagg
    15181 acatctggcg gaagtaaaac tgggaaatga agcagcttac gatgaaacag acaaagccca
    15241 agtgacttat gatggaaaaa tctacggaga acctgcagta atcgagaccc ttgttttgta
    15301 ctataacaaa gatttagtcg atacagcacc atcaacgttt aaagatttgg agaatctatc
    15361 caaagacagt cgttttgctt ttgagtcaga agcaggaaaa aatacaggat ttttagctaa
    15421 atggactgac ttttattatt cctatggttt aattgcaggt tacggtggct atgtatttgg
    15481 tgatgatggc acaaatcctt cagatatcgg tttgaataat gcaggtgctg tagaaggaat
    15541 ctcttatgca acagattggt tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag
    15601 tgcaggagac ttcgccagcc agcaattcat gtccaataaa actgcagcaa tcattgacgg
    15661 tccatggcaa gcgcaaacat acaaagaaaa caatatcaat tatggtgtag ccaaaattcc
    15721 tactctaaat aatggacagc cttatcaacc gtttggcgga ggaaaaggat gggtagtcag
    15781 caattatgct aaaaacaaag acctctctca aaaatggctt gattatgtaa cgaatcagga
    15841 aaatcaagaa aaattctttg aaatggtaaa tgaaatccct gcaaaccaac aagctaggga
    15901 aacagcaaag gctaaaaacg atgaattaac aacagctgtg attgaacaat atgaaacagc
    15961 tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag aaaacttgat
    16021 gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac
    16081 aatcagtgaa gccatagaac aaaaatactg attaaaatga taaccgtcca tttttataaa
    16141 aaatggaaca ggtgcttcag ataaaagtgg caagagcgag atggaaaatt atcttgctct
    16201 tgttgccatt atcttaaaag cagtttgcgg tttagcaagt ttagaggggg cattcagatg
    16261 cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc acttattcca
    16321 gggatggggc agtttgtaaa tggccaagct tttaaaggta tcgttttttt cgccatattc
    16381 gtcctattca ttggtcagat gcttctgggc ggatttcatg cattggtagg attggttact
    16441 ttgggaagta taccaatgga agatcattca ttatttattt tgattgaagg cactttgcaa
    16501 ttcatcatta caatcatttt cttgattttt tacggtttga atcttttaga cgcttatcga
    16561 gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga agtcatacat
    16621 aatataggtg atgaaggttt tccttatcta ttgacacttc ccgcttatct tctcatgatt
    16681 tttacgatca ttttcccagt cctagttaca ttgttcactg catttactaa ttatgacttc
    16741 aatcatattc caccagccag tttaattgat tgggtagggg ctaaaaactt ttttagtatc
    16801 ttctttttaa gctcatatcg taatactttt tttgcagtct tttcgtggac cgtgatttgg
    16861 actttatgtg caacaacttt gcaaatcacc cttggtattc tcacagcggt tgttgctcac
    16921 cagaaattta ttaaattcaa acgtattttc ggtatcattt tcttattacc atgggcggtc
    16981 ccagctttta ttactatcat gagtttttcg aatatattca acgatagcgt aggtgccatc
    17041 aatactcaag tgattccttt cataaaccag cttccttttg tagatatcag tgcaatctct
    17101 tggaaaacgg acccgaactg gacgaaagta gcaattatct tagtgcaggg ctggttagga
    17161 tttccctata tctatgtgat ggttacagga atcttacaat cgatttcaga agacttatat
    17221 gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct tttcaaacat cacgcttcct
    17281 gctgtcttcc tagtggcagc gccaacattt attacacagt atacggggaa tttcaataat
    17341 ttctccatga tctatctgtt taacaatggc ggtcccggaa gcgtaggcgg aggagctggt
    17401 tctactgata ttcttatttc ctggatctat aaacttacga ctggaacttc tccgcagtat
    17461 tctatggctt cagcaatcac gttgattatc tccgtcatcg ttatctgtgt gtcattgctg
    17521 gtatttaaaa agacaaatgc gttcaatatg ggggattaac aatgaaaaaa tttcattcga
    17581 gtgctcgatt ccaaaaacga ctttctcact tttttactta cttttttctt ttaatactgt
    17641 cagtcattat catctatccg cttttgatca cagcaagtac tgccttcaag tcaggaaata
    17701 ttgcagcttt tagtttagat ctttcaagtg actggacttt aggaaacttt actcggctat
    17761 tcagtgaaac actttacggg acttggtata aaaatacatt gatcatttct gtcctaacaa
    17821 tgatcgtaca agtgactgta gtaactttgg cgggctatac ttatagtcgc tatcgtttta
    17881 tcggccgcaa atcaagtttg atgttctttt tagtgattca gatggtaccg actatggcag
    17941 cattgactgc cttttatgtc atggctttct tattagaagc gctcgataag tactggttct
    18001 tgacaatgat ctatataggc ggtggaattc caatgaatac ttggctgatg aaaggatact
    18061 ttgacactgt accaatagat ttagatgaat ctgctaaact tgatggggca gggcatttca
    18121 ggattttttt ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt caagcattat
    18181 gggcttttat gggacctttt ggggatttca tgttagctaa gttcctgctt cgaacacctg
    18241 aaaatcagac tgttgctgtc ggcttacaaa catttatttc tgatgtgaca aaccaaagag
    18301 tctctctttt tgctgctggg gcaattttga ttgcactacc gatttgtcta ttattcttct
    18361 ttttacaaaa gaactttgtt accggcctga catctggcgg aacaaaagga taggagcaga
    18421 gtatcatgca aacatttccg ttaaactatt tttccgcatt acgtacgcca actcaagtgt
    18481 ttgcgggacg caaactatta tcatggccta aatttttctt gatatttgta tttttagttt
    18541 ccttgatggt catgcctgtg actctttttt atgccaatca gatccaagcg attccattag
    18601 aacaatttct atccgttcat tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac
    18661 ttgaattatc tgaaactggg ttacaagcat cgcaacaaac gatagctgtg acccctgaaa
    18721 ttttggtagg tgtgtcttta tctgagaaac aacaatccga tcatggaact tttattgatt
    18781 ttgagaagga acaatgggtg atccaacaaa aggataaatc tggtatacgc cgctatacga
    18841 tgaattatag cccatctttc caaccagatt ctgtacgtac gcccgaagat ttccaacgat
    18901 ttttagaaag agaattttat gccagtaatc gtccaacaat cattttatct tatagtttaa
    18961 gtcttggtct tgtcctattt gtcatgacga gtctcatttt attcggggct tcctttttct
    19021 tgtggatgac aagaaaaagc caactttcat ccattcatac attcaaagag agtgcaaatt
    19081 tgatgctgaa tgttatggga ataggttcca tggcagcagc agttgtaggg tttatccact
    19141 ttgactttat cttgatgcta agtgtccaaa caacgataac tgtcttgctg cttttatggg
    19201 tttttgtcaa aacaagattc aaagataagc gtgtcgaatg acaagtttaa gcgatttatg
    19261 cgacaatgaa tgtgaaaaaa atgaaaaata aagtagtttt tgaaaaattt ctgaaaaaat
    19321 taaacttctt agttgattct tttctttttt agtattaacg taatagggta gtttttttaa
    19381 ggaggaaaat catgttagag aaaactttat acaatcaatt attttcacgt tctttctcgc
    19441 tccctgtcga agtaaattat tgggacggta caacgaaaca atacggagat acggacactc
    19501 ctccccaaat caaaatcaat atccatgaag aaatcccaat gaaagagatt acaagtaacg
    19561 catctttggc actaggagaa gcctatatgg atcatcgaat cgagatcgag ggaagtatcc
    19621 aagcattgat caacgatgct tatcagcaag caaacagctt tttacgagga aaagattatc
    19681 taaaatggct gccaaagaaa gaaaaacata cgaaacaaaa aaacaaagaa gatatccatg
    19741 cgcattatga cttaggtaac gacttttata aactttggct agatccaact ttgacttatt
    19801 cttgtgctta tttcaataca cctgaagata cgttggaaca agcacaaatc aataaagtcc
    19861 atcatatctt agacaaactc tttatcaaag aaggcgaaac gttgcttgat atcggttgtg
    19921 gctggggaac attgatgttc actgctacca aagagtataa tgtcaaggca actgggatca
    19981 cactaagtga agaacaatac gatttgatta ccaaaagaat aaaagaagaa catttagaag
    20041 ataaatgccg tgtattatta atggattacc gcgaactaaa aggcgaaacg tttgatcata
    20101 tcacaagtgt aggtatgttc gaacatgtag gttctgaaaa tcttgaaggt tatttcaaag
    20161 tggtaaaaga tttattgaaa ccaaaaggaa cagctttgat ccatggaatc agccgacaac
    20221 aaggcggtgc gacaaatgct tggatcaata aatatatttt ccctggcggt tatgtccctg
    20281 gtgttactga attagttgat aatattacaa aaaacgattt gcaactgatt gatttggaaa
    20341 gcttacgtcg tgactaccaa ttgacattag aacattggac aaagaatttt catcaagtcc
    20401 aagatcaagt cacgaaagaa atgggtgatt ccttctatcg aatgtgggat ctatatttac
    20461 aagcttgtgc agcttcattt gaatcaagta atatcgatgt gattcaatat ttgcttgttc
    20521 aacctggaaa caatgacatc ccaatgcatc gtgcataatt gaggcaaaag tactgtttaa
    20581 agactattta aaaagaagct aagttttatg atgctccaga agtgaacttt tttgtctaac
    20641 ttctaggatt gtctctaaag cttagtttct ttttctttat attttcataa taaaagtttg
    20701 gttctttaat ttttaaacat gacagaaaat tttttcttcg ttatacttgt aatagaatac
    20761 tattttataa caaaggggat cgaatcaatg aaaaaaactg ttatttatac tagtttattt
    20821 gcagctttgt ttttagctgc ttgtcaatca aataccaatc aaggcacatc tgggaatacg
    20881 tcttccagta gcgaacagcg ctcttcacag gtatcggact cctcaacaaa agcaagcaca
    20941 tcttcgtcta gccagacaaa aagttctagt gaagcaagta ctcagtcaga tatcaaagtg
    21001 tctgcagctc gtgcgatcga attgttccaa gataaatacc cagatactgc aattactagt
    21061 ttggaattag atagtgattg gggatcttac ttctacaaga tagaaggtgt cgatgatcaa
    21121 aaagaatacg aagcaagaat tgatgctcaa aatgaaaaaa tggagacaga gaatccagaa
    21181 actctagatc gagatgaaca aaatggtcaa aagaaaaaag aagatggtct ggatgttgat
    21241 aaattgatca cgattgagga agcagcaaaa atcgccgtcg acaaagtagg aagcggtacg
    21301 gcaacagatt gggatttgga taaagattta ggtactactt attgggaagt caaagtaaaa
    21361 gacggcaatc aaagtacatc agtcaagatc aatagccaaa caggcgaagt actgtcaact
    21421 gaacaagatg attaagaatt tcttcggaaa acaagagttg atttttcttt tagtcatgat
    21481 acactagaca aaataacttg tttgaaggag cgatttactt gagtttagaa acgacctttg
    21541 ccaatcatat ctttgccaat cctttgatga atgcatctgg tgtccactgc atgactaccc
    21601 aggaattaga tgaactggct cattcagaag caggagcatt tatcactaag agctgtacga
    21661 tcaatgaacg aaaaggaaat cccgaaccac gctattttga tgttccttta gggagtatca
    21721 attctatggg acttccaaac ttaggttttt cttattatct agagtatgct cttgcgtatg
    21781 aaaaagtgca ggaaaaccaa aatcaaccgt tatttttttc cattgccggg atgagtgtcc
    21841 aggaaaactt ggaaatgctt gagaaaatag aaaaaagtgg gtttaatggg atcactgaat
    21901 taaatttatc ttgtcccaat gtgcctggaa aaccacaact agcttacgat tttgaggcca
    21961 cttatgaaac actgaaagaa gtattcagca tcttttctaa gccgctagga atcaaattgc
    22021 ctccttattt tgattttgct cattttgatc agatggctga tatcttgaat cagtttccat
    22081 tgacctatgt caatgctatc aacagtgtgg gcaatggttt gtatatcgat acagaacaag
    22141 aagcagtagt catcaaacca aaagaagggt tcggcggcat tggcggagag tatatcaagc
    22201 cgactgcttt ggcgaatgtt cgcgcttttt atacacgttt gaaaccagaa atccaaatca
    22261 ttggtacagg tgggattcgg acaggacaag atgcatttga acatctgcta tgtggcgcaa
    22321 gcatgttaca gattggtacc gagcttcata aagaagggcc ggaaattttt tctagaatca
    22381 taaaagaatt aacgcagatt atgtcagaaa aaggatatac gtcaattgac gaatttaaag
    22441 gcaaattacg caccatttca tagcaaagag acgaaggtca tgattgatct tcgtcttttt
    22501 ttataaaaaa cactcgtctg ttcattcctg tgcaagcgta ctcattgtat gttagacgaa
    22561 cttgtgggaa aataacagag gcaaaaagga ggataatatg aatgatgcaa cagaattagc
    22621 aaaagagctt aaagaaaaag aatttagttt tcaagaacta gttacggata tacagaaaaa
    22681 aataaagaag caaaatcatg aattgaatgc gtttgtcgcg cttgaaccat tcgagagtaa
    22741 ttcccacgag ctattgattc cccagtcaga aaaagggcca ttggctggta tcccatttcc
    22801 tttaaaaatg ctcggtcaag aaaaaaaggg atggctagct acttcaggat ctcgtttatt
    22861 tgaaacacac cgagcttctc atacatctaa ttatgtgcgg caagcagaag cgataggtct
    22921 agttcctttt ggacaaacca atgcgccaga gtttggtttc aaaaatatta cagacccagt
    22981 catctatggc ccagcaagaa acccttggaa ccttgaatgt acaccgggcg gatcaagtgg
    23041 aggagctgct gctgcagttt catcaggaat cgttccttta gctggtgcta gtgatggcgg
    23101 tggttctatt agaatacccg cttcgttttg cggattgatt gggctgaaac cttcgcgagg
    23161 gacgatgcca gttggtccat atgcttggag aggatggcaa ggagctgcta ttgatttcgg
    23221 cttgacagtt tctatgagag acacggaggc tttattttat gggatgagaa gtatccattc
    23281 cggtgcgcca tatcaagtat cgccagttga gtggcaaaca catccaagaa aaaaacggtt
    23341 gaagatagct ttttgtgttg attcaccgat ccactcacag attagtgaag aggcagtact
    23401 tgctgtaaaa aaagctgtag atttccttgg aaaagaaggg catgatttga cagagattcc
    23461 ttacccacta gatggtcgta aactgatcac gtcttattat cagatgaatg gcgcagaaac
    23521 agcagcaatg atccgctcga ttgaaacagg catccagcga gctgtcagaa aagaagaact
    23581 tgaacccatt tcttggactt tgttccaata tggtcagaag ctctctgccg ctacttacat
    23641 cgattctctg catgtatggg atgaagcagc aattgtcatg gaaaacctac atcaagaata
    23701 cgatctattc ctttcaccaa cgactgcatt taccgcccct aaaatcagcg aagatttgca
    23761 aagtgatgcg ataagagcac agatggaaca catcagtgat tgttctgaag tagaaggttt
    23821 agacgttgtt tcttccatgt ttgaggagag cttgaagatc acaccttata cacagttggc
    23881 taatctgact ggacaaccag caatcagctt gcctactcat ttgacaaaag aaaacatgcc
    23941 tctaggaatc caatttatgg ctgccagagg aagagaagat ttgctatttc aaatcggcta
    24001 tctatttgaa gcaaatcatc agttccattt accaaatcca tggaaatagc agaaacaagg
    24061 agaagaagaa tgaagaaaaa aagattgttt gctctaataa ctagtgcctt ttttatttta
    24121 agcgcttgcg gtacagctgc cgctaatcag gaagataaca gctggagtaa tgtagaaaaa
    24181 aagaaagaat tagtagtagc gacttctggc acactgtttc ccgcatctta ttataatgat
    24241 caaaatgaat tagttggtta tgatgtagat ttggcaaaag aggtggctaa acggttaaat
    24301 gtcaaaatct cctttaaaga atacaacgtg gatggacaaa tcacttctgt ttcaaaaaga
    24361 gaatcagatt ttgctgcgaa cgattttggt atcacaaaag aaagagaaaa aaaattctcc
    24421 ctttcagaga ctttcaaata ttcatttgac agtatgatcg tacgaaaaaa agatgattca
    24481 ggaatccact cacttgaaga tcttaaaggg aaaaaagcag ctggcgaacc caatactagc
    24541 tatatgcgtt tagcggaaaa acacggagct aaattagtca catatgacaa tgccacgaat
    24601 gatcagtatt taagcgatgt agcaaatggt cgaacagatg ttattctaaa tgattattat
    24661 ctgcagaaaa tggccgtatc tgctttacca gatatccctg taaaaatact ggagaacgtt
    24721 tatttcaatg aaaacgaaag tggtttttta ttgaaagaag gaaatgatgc tctgaaaaag
    24781 aaaatcgatg gcgtattaaa agagatgaaa gaagatggga cgacaaagaa aatatctgaa
    24841 aaatattttc aaactgatgt ttcagtcaaa ccgaaagaaa cgatggctga gtcggtgagt
    24901 gagtgatgta ctttccccta attgattttt ccctcatgtg gacttcactt cctttcgtac
    24961 tgcaaggctt ggtatacaca ttagggattg gcttcgtgtc gtttctttta ggaaatttgg
    25021 tagggttgct tttgacagtt ttaggtcttt tggattggct gcctctgaat gtatttattc
    25081 gtttttattt atcgttcttt agaggcattc ccgcactagt cctgctgttt ctgttatatt
    25141 tcgggttacc ttatcaatta agtgccttaa cggcttcagt catctgcttt acgattacca
    25201 gcagtgcatt tattggtgaa atctatcgag gatcactagc aggcgtttct tcaggtcagt
    25261 gggacgctgg tttagcttct ggtttttctt tctttggggt attgaaatat atcgttttgc
    25321 cgcaagcttt tcgaatctca gtaccggcac tcagtaacgt agctatggat ctagtgaaag
    25381 gtacttcgtt agcagcaaca atcacagtgc cagaaatatt tcaaaaagca aagatcatag
    25441 gcggtcggac attcgattat ttttctctct atattttagt tgcactgatt tattgggtca
    25501 tttgtatcgg gatcgaaaaa gtgcaacagt atttagaaaa acgttctatc catcagtcaa
    25561 tctgaaaaat agaaatggtg ctagcgtggc tggcaccatt tctatttttt gtttcttgta
    25621 tattctgcct agtattttaa aaccatttca tgtaaaatgt aaatcgaaaa tatgttcgga
    25681 ggtagaaaac atgaagaccc cgctaaccat cgaaatagaa tcctcgttat accaatattg
    25741 catcgagcaa ggcggcttgg ttgtagaaga agtcacgatg cctgatgacc aaggaattgt
    25801 agatacgctt agctgcttga caaaatttga tggaagccga gaatggcgat gctatgagct
    25861 gaaagtgaca aaagcagatt ttcgctctac agccaaactg tcatttgttg gacattataa
    25921 ttattttgtt ttaccaagta aactatatga agaagtaaaa actgaaatcc ctacaggaat
    25981 aggtgtttta gtgtatcgtc cgtacacggt tgaagaggag atgcctgcct ctggtacatt
    26041 tctgattgcg aaaaaagcag caaagaaaga gcttgctgta ccggaagaag ctttgacaaa
    26101 ccgcttcatg gcttctttat tcagggaagt aagaaaagca aagcaaatgg agcgaggcgt
    26161 aaaatatttt ccaaaagatc aattgtacaa agaattgaaa agacgttcag aaaatcaaga
    26221 tccattttct aacgaacgat attatgaacg gtttttagag gaaatagcta gtcaggcaat
    26281 caatgagttg caagaagaag ttatcgcgct tcagcatgat tatgaatatt tgaggcaaca
    26341 acagcagatc cgtcggttgc ctacagagcc attggagtga aaaatatgta ctatccttat
    26401 ttaagaggaa aacaatttga cctattagca ttaaaagaag cactgtcgcg aggattgctc
    26461 tccaataaaa tccaaccagt gatcgaacca gtacgtgatt ccgctacgct taaaaatgtg
    26521 atcgaattat ttcaaaaaaa gcaccatcca atcgctgtga tccaaaatcc ccaagtagga
    26581 caattcaaat tattcgatca gcatgtgcac agttggacag tcaaagaaaa tagctctgtc
    26641 gtccctgcac agatcttaac gccagaaaat ctcagtgaag tactggattc acctccagcg
    26701 tttcttgttt tcgatggaca gcactaccca aaagatacag aggtgtggaa acagttggca
    26761 ggagttgatt cgaaattttt gataccagat actagtcgct ttcgcatctg gctgcccgaa
    26821 aacaaaattg tgatccgtga ctcttttcaa acgagaaagc atgttgaaag ttatgcagat
    26881 aaaacagatg acttttttag tgatgactat ctttttttcc acgcagacgg atatattgga
    26941 ttttctgatt tcacgatcga aggtagccgt tatttcgata aagggttccc tagccgagcg
    27001 cttgctatcc atctaacgta tattgatgct tacggcaata tacgcgtgaa acattttgtc
    27061 tctgacagta acgactctgc taaagaccaa gcattgaagt ttcttgaagc aggagataaa
    27121 atgaaaaaat ggatcatgcg tcaccatcat caactcttga ttacttcagg aatgattgaa
    27181 ttattggctc tttaccagca acaaaaattc cctggactgg gagtgttgaa aaagtggtca
    27241 cttatgcatc atttggaact tattagtcac ctgctagatc atccaaaaga ttggcttaaa
    27301 tattattcaa aagttcagga actatatgaa gtgatccaat gattagataa tgtaaatgaa
    27361 aaaggaatag aaaagaggaa actggatgta tattcggcaa acaaggaaag aagaagtaga
    27421 agaagtggta gaaatcatta atgccgcagc ggatttcatt gcttcgcaag gctctcctca
    27481 atggcaaaac gggcagaaac caacaaaaga aacgattttg aacgatattg aaagaaaaga
    27541 aagctatgta ttaatagtgg aagatcaaat tgctggaaca gctgcattag tctcaggcgt
    27601 ggatcccgtt tacacaaaaa tagatggtag ttggaaagaa tcaactggtc cttatttatc
    27661 gattcatcgt gtcgctttag cagaaagtac tcgaggaaaa aaactagcaa aaccgttctt
    27721 agaacatttg ttgacgattg cagccagcaa aaatatccaa gatatccgta tcgatactta
    27781 tccagctaat gaaccgatgg aaaagaccat ctattctgct ggattcactt atcaagggat
    27841 gattgaattt ccttttgaac acggcgaaag aaaagcatat caaataatca tcaattgact
    27901 ataaaatcat ctgagcaaag ataaagacat taacactact gaaaaaaaca atcatatgat
    27961 agccaagagg ttgtaacaga agtggccagc ttcaaaaaac aagaaagaat ttccgaaaat
    28021 gcttctcaaa tttttgtgga attttagcgt attttacgaa atagctgctt tctgaaactc
    28081 ccttcatcga gaggtcgtga catagctttt gtcacgatct ctcggcttat cctacgagta
    28141 ttattcgata catttatatc aataatcctt tttttatttt atctgcagct tcttggaacg
    28201 ctccatcgtt tgacacgact aaaagtgagg ggatagcttt caaacgtttc aactgtgcta
    28261 aatcttctac ataaaccgcc agtctggctg aaatctgttc agatgaatct ccacgttttt
    28321 tcaggcgcga ttgcaatgtt tctttagaga tcgtaataaa gataggacgg actaccgtcc
    28381 cgaaatactc atataaatgc caaaaaccat ttgctgtcaa aacggcgtaa caatttccta
    28441 ctgtcagttt ctttttaatt tctgctttac tggaaccata tttatgttga gcatattggt
    28501 cccactctgc aaactcattt cccttgatca tctgattgaa tgtattgtca gaaacaaaat
    28561 aataatcctc ttgatctttt tccccaggtc ttggtgatcg attcgtgtaa gtaatgatct
    28621 tttgttccgg ctgaaaaata gcttctgcga gtgaagtttt tccagaacca gaaggaccga
    28681 taaatacata gcaataattc attgtcattt ctcctttgat gattgaacaa gtgaaaaagc
    28741 tttggaaaat atttccaaag cttttaaaag taactttgct tttttgtatt ttataccagc
    28801 aagccagtca caatagcact taaaaagctg actaaagtcg ctgttagcaa aattttcaat
    28861 ccgtgttttg cgacaagatt tcctttttct tcatttaatc ccttcatcgc accagaaatg
    28921 atcccgatag aagagaagtt ggcaaatgaa acgaggaaga cagatataat tgctgttgta
    28981 cgctcagtaa aatggaaatt accatttgct aattctgtca tagcaacaaa ttcatttgta
    29041 accagtttag tcgccatgat actaccggca tccactgctt ctttccatgg gattccgctg
    29101 atgaaggcaa gaggggcaaa gatgtaacct aggatctctt ggaaagtgat gccgaatatc
    29161 ccgtggaaga tagcattgat catggcaatc aaagcaacaa aaccgataag catagcagca
    29221 accgtgatcg ccacatggaa accatcaaga atgtattctc ccaacatttg gaaaaatgtc
    29281 tgttctttgt tttcttcaat gatcagctca tcttcttttt cttctaatac atagggattt
    29341 acaattgaag ccagaatata accgccaaat aagttcaaaa caagggcagt gatcacatat
    29401 ttagaatcga tcagtgccat ataagatccg acgatcgaca tagaaactgt agacatagcc
    29461 gatacactca tggtgaacaa acgtttctca gataggaagg gtaattcctt ttttatggaa
    29521 ataaaaactt ccgattgtcc taaaatagca gaagctactc cattataaga ttccaatttg
    29581 cccatcccgt tgattttact taatcctaga ccaacagctt tcataaataa tggaagaatc
    29641 ttgatatagc gtagaatacc gataatagca gaaataaata caattggcat caatacggaa
    29701 agaaagaaag gtgcacttcc tttatttgct acaccggcaa aaacaaagtc aacaccttgc
    29761 ccagcaaaag caagaagatg atcaaacatt tttgccaata cagaaacaac cgctgtacca
    29821 aaagttgtac gtaataatac gaaaccaaga acaagctgta aaacgaacat cacaattagt
    29881 ggtttcaatt tgattgcttt tttgttactg ctgatcaaaa aagcaagccc taatgtgaca
    29941 aataaaccaa taatacctaa taaataactc aaataatcac tcctattcca aattccgttt
    30001 tattttacct gttttgcaaa taaaatacta atctttagtg aaattttcat tactttttta
    30061 gagtgaaacg cattcatttt ccattagaaa ataaagcgaa caaaataaaa ggtttaaaaa
    30121 gtgtaacgtc ttagtaaagt ctataaaaaa acgaaactat cgattaagct aatagtttcg
    30181 ttttcaacat ttataagtaa actttttcta ttggtaatat agattttcgg aagggtagac
    30241 aggtgcgcag gtaatatcga ttcctagttt tttcaatgtt tgttcatcat ttttattgac
    30301 catcacagta gagtgggctt gtgctccctc aagttcagct agtttatcat aagcaacttg
    30361 agcgattgga ttcgttactg cactgattga taaagcgatc aagacttcat tgatgcttag
    30421 tgcatccaaa gaggaatgaa gtgtctttga tttcaatcct tgtattgttt caagaactaa
    30481 tggtgacaat aaaggaatgt catctgcgat atgggctaaa tgttttactg catttaagat
    30541 aactgccgct gacgcatcca tcaatgaact tgtacgcccg gtaatgatga cttcatctgg
    30601 taattcgatt gcaatcacag cagaaggatc ttttgtagga ctttgcgctt gaatcttatc
    30661 tgcatagtca tgggcaggga ctaccacgtt tcggtcttca ggacgtaaac cgacttcttc
    30721 cataatcagt ttgattcgat taactgcttc ttcgtcgatc aatccttttt tgaagtcgca
    30781 ttctgtttgg aaataacgac ggatgatttc ttgtttagag gcttcttgga caacggtatc
    30841 atccacgatc ccaaatccta cacgattcac acccatgtca gtaggagatt gataaacaga
    30901 ttctttacca gtaatttttt caataatacg tttgattaca ggaaatgtct caacatcccg
    30961 attatagttc acagctactt tattgtaggc atcaaagtgg aaggaatcga tcatattgac
    31021 atcttttaga tcgactgtgg ctgcttcata agcaatgttc agcggatgtt tcaacggaac
    31081 attccatact gggaaagttt cgaacttgga gtaaccagct gcatgtcctt tttggctttc
    31141 atgatacagt tggttcaagc aagtagcgag ctttccactt cctgggccag gagctgtcac
    31201 tacaacgatt ggtttagttg tttcaatata ctcatttttt ccaaaaccat cttctgagac
    31261 gattttttct acgttgactg gatagtcttc gatttctgag tgtttataga ccttgatgtt
    31321 tcgtctttct agtttattga taaagacttt cgtggcaggc tgtccactat agcgagtgat
    31381 aacgacactg ttgattaata agccataacc acgtaattca tcgattaacc gcaagatatc
    31441 catatcataa gtaatcccat aatcaccacg gatcttatta cgttcaatat ctccagcata
    31501 gacgcaaatc agtatttcag cttgatcttt caatttttgc agcaatttga ttttcgcatc
    31561 ttcttcaaat cctggaagta cacgtttcgc atgcttgtcg tcaactaact tcccaccaaa
    31621 ttctaaatat aatttgtcat agtggttcac tcgttccaaa atatacgctg actgctcttc
    31681 aatatatttt tctgaatcaa aaccaatttt tttcactagg ttccctcctt aagtactaaa
    31741 gcattttttc atttttttga ggattcgtca actggaagag cgaaattcct caaaaaaaca
    31801 aagaaaatgg tttaaaccaa cttgtctaga tagttattca ttggtatgat agtttttgat
    31861 aaggaaggtg ctacatgaaa aaaagacaga aaaagaaaaa cgcatataaa cactatatcc
    31921 gttcgatttt taccggttat gaaagaatgc ttgaagatcc cgaattggaa caattgacgt
    31981 ttacttatct aaacgaagaa acgcagctaa ctcgtgatga gcatcagcgc attcatttca
    32041 caacaagaga tctgcctagt aaataaaaaa taagataaag gtcatcggat tcgattctgt
    32101 cctatcagat ggaaagacta gttgatcgaa aaaaaggctg taccaaattt tggtgcagcc
    32161 tgttttatgg agcattgccc gctctttttt ataacaattt tttacagaaa atggtactat
    32221 tgaagtatta ataagtgaaa aacagatgag taagaggagg caagtagtat gaaagagcca
    32281 gatacaaatg caaattatga gccagacgaa ctagaggaga cgaacgcgga tcaggataca
    32341 ggacaagaat cgtcttcttt ttacgatatc aaagaaaaag caagcatgaa tctttcccaa
    32401 ttattagaag acttacaggc ttttgaaaaa gctgtccgcg aagaagatat ccctgagatc
    32461 taccgtttat acaatggccg attgaatgaa gaattaaaaa gaagctcaaa tgaaaatcac
    32521 gagatcgatc aattgctgat gcgtaagatc catgatcgtt ttttacagga atttcctttt
    32581 atggaacaag tagaaaacat ctcgccaacg atgagctatt acaaaatcgg cacttattac
    32641 catgatcggc ccacggtcgg tattgatgca agtctgccag aaatttttgt tttgcctaag
    32701 atcgatgaag agtgggagaa atattcccaa ccggagtcaa cggactatga caaaaaaatc
    32761 aatgagctag acgcaaaggt gatcactgct cagacagaaa ttgaacgttt agatgaacaa
    32821 ataaaagaaa tcaatcgaca aatgactgat ttgaacgaca ataaaggatt cttgaatcgt
    32881 aaaaaaatcg aggaagaaat tcaagaatta gagaagaaga agcaagtact atctaatgaa
    32941 aaattaggct ggcttcctta tattgagacg cctgaaacaa tccagcagca aaaagaagca
    33001 ttgaagcaag aagcaagagc agaccagctt cgtgcagcca tcgtagaaaa agaacaaagg
    33061 caaatcagac gctatttcgg tagcaaagag ggattcggtc aagcaatcca tgaattcttg
    33121 atgaattatc ttggcaatga gaatcctaat aatcaatcta atgaaggagg ttccgagtat
    33181 gaatgaacac gatgaagcag aatggacaaa aaatgaagaa caaacagaag atcatgaaga
    33241 aacgaacact tcattggaag aacaaacaag taatcctctg gatgacacag aagaaatccc
    33301 tatgctagag gaaacaaaaa tacatgaaaa agaaaaagta actccgacga atacgaatac
    33361 tgcagaaaac acccatgctt acgaaataac tgaaaatccc gggtatttag aaggaaaaga
    33421 gcaagatcat caagatatcg tcagtttgat tgaaacagcg gaagcagaat tagcggattt
    33481 acgtatgaga aaagatttga tcgaatcaga ttttccacaa ttattagaat catatgatcg
    33541 ttttatattg aatgaagaac caatctccac gatcggaaag acaagcctga tcgaatactt
    33601 cacgtatgaa ttactgaaac cgttgaatga tattggtctg gaacggtcaa attcagctta
    33661 tgacacatgg aaaacaagac atttttcgat caattatacg ttgaatggac aaaacgaatt
    33721 agtcttttcc tttgtattac cgaccatcga tcaacgatac caagtggaat cgatgagtct
    33781 cctaaacgta tcaccagaga caatggaaat caatgtccaa gatgaccggg tgctttcttt
    33841 gatccgacac tggtcagtgg atcgagtatt ctctgcaggg cagatcacga tattcaatca
    33901 caaattgaat caattattag cccatgcaag aacacttggt tttaccgtta atcagacatt
    33961 actagacaat acgaagccac ttcgtttgaa tcttcagagc gaatttgaat tgacgaatga
    34021 agtattggat gatattttta tcgtggcaat gaaaaatcca tcctacgata tggaaaagat
    34081 caacgaaact acttatcaag tacgattaga taaaggacag tctttaacga taacaacaaa
    34141 cgaaaaagat caaacagtct tgtcgatctc atctggagac tacccacgat ctgtcattga
    34201 tttctttatc aattacgaat tcttggtccc attaatggtg agaaagtcct gattggataa
    34261 gttggattac cagatgtcat tcagataatt ctgttccaaa ggacttgcgg cagttatcca
    34321 gaataattgt cacaagtctt ttctgtatgt actaaatgga cgtgaataac ttgctgatga
    34381 aggagggatt tcattaggaa tgtccacaaa taaaatagca cagcttagct tattgtctgc
    34441 tgcttgtgta gcaggtagga tcgcttttca atttattccg aatttccagc caatgactgc
    34501 tattttttta tttatagtac tttacttaag cttaaaagat gcgttaattg tcatgagtct
    34561 ttctattgcg atcagcagtt tttattttgg tgttggacca tgggtcatcg gccaatggat
    34621 cagctatact gttgttctga gtttgttttc aatcgtctgc ttgagaaaaa cagtgcaaag
    34681 gaatctatgg ttaaagggag tctgtttttt tctggcgggc atggtttacg gaataggtat
    34741 gaccgttttt gatacgctgt tatatcgttt gcctcaacct tggatctatt accttcaagg
    34801 ggtgtcgttt gatttgatgc acagtatagg caatgttgtg ttctttcttg tttttctgcc
    34861 agtcgtcaaa cgtttttaca atcattcagg aggaaaaaat gaaaaaaata atttatagtt
    34921 tgcttgcaat gagtgtcagt atactcattt tgacaagttg cagtacaacc tatacacaat
    34981 cggcttccaa ccaaaaggag gaagtaaaaa catcgcaaaa agcaacaatc acgttacaag
    35041 aaaatggtca aaacttttcg gaaaaagaag tggtcttcaa aaaaggcgat gatttactga
    35101 gtattttgaa acgaaatttt gaagtcaaag aagataacgg cttcatcact tcgcttgaag
    35161 ggcactcaca ggatgagaaa aatcaactct attggatgtt tactgtggac gggaaaagtg
    35221 ctactactgg tgccaaagag atcaaattga atgatggaca atcggtcatt ttcaatttgt
    35281 ctaaactata agtttcataa aaaactgtta aagggcgaat attgccataa ttcagtgagt
    35341 tcagttgatg aaagaaggaa aataacatgg aaaattttaa aaaaagcgga atgttttccg
    35401 taattgctgc tctaggggct aatattctag ttgctatcag caaatttgtc ggctatgcaa
    35461 tcagtggcag tgccgccatg ctaaacgaaa gcatccacag tatcgttgat tgcagcaatc
    35521 agattttgct gttattcggt gataaacaag ccaataaagg acagagcgag ttgcatcaat
    35581 ttggtgaagg acgagcaaaa tactttttca gtacaatcgt cgcaatgatg cttttcttcg
    35641 gcggtggggc acttggggtg atggaagctt ttgaaaagtt gacgcatcca tcccatgaag
    35701 tgggtaatcc gatcattgtt atagcaatac tgttatttgg tttggtaatc gaaggaagtt
    35761 cgttacgcgt ggcaatgaaa gaaatcaaag aattgaatac tgaaaaactt tccacgatgc
    35821 gctttttgcg cgaaagtcga cacagtgaaa tactgattat ttttacggaa gacttgtgtg
    35881 ctgtaatcgg tttggtcttg gctttagcag gaacaatatt gacttatgca accgggattg
    35941 ctgcatttga tgcgatcagc ggattgctga tcggtttctt gctgatggga gccgccattt
    36001 tcctagcaaa agaattttat agtctgatca tcggtgaaag cgtgacagca agcgatttat
    36061 caaaaatcaa gttagctttt gaacgtcctg agatcagtcg gctgatcgat ataaaaacag
    36121 ttcatttaag tccaacggag attttagttg cagcaaaagc agatatcgtt ggtaacaaag
    36181 agaatgaagc ttactcggtc atcaatgata ttgaaaaaaa tatgcgtcag tctcttcctg
    36241 acaagaagat gtatatatac attgaaacag ataaatatga cccaaattat tctcgtaaat
    36301 aaaacgttta ttcatgaatt gaagcaaaaa tgagaaaaat ataatcttgt ttttaaataa
    36361 aacggctaca atttagcgaa tttatgttac aatgagtccg ctgtttattt gttgtaatta
    36421 aaggagatat tttaatgaac aaaaagaaaa gtgcgccatg gcttcaagct ttgcatgcag
    36481 tgatgccact ttgtataagc tatataccag taggattagc ttgcggagta ttgttgcaaa
    36541 aagttggatt tgatcctctc ttatcaggac tgttatcgat tctggtcttt tccggtggcg
    36601 cgcagttttt agttgcatct atgcttacga cgcaagcatc atttgcaacg acattattga
    36661 tggttttctt tttagaatta cgctatacat tattaggagc aagtcttgcc ggttttatga
    36721 aaaaagaaaa gcggacgttt ttggctgtct tttctcaatc actgaatgat gaaaactatg
    36781 cggtgaacta cttaaagttt tcaactgacc cttcctggaa taaaagaaag gctttatatg
    36841 tcaattggtt ctcaatgact gcatgggccg gtagcaatat gatcggtaat tttttcggct
    36901 cggtgattcg agtagatgct gaccttgtcc attttgcttt gacggccatg ttcattttca
    36961 tgtttatcat gcagatgaaa agcgcactac tgatctttac aggtttattc tcaggcgtat
    37021 taggggtcgt attcatggta ttattccaaa atactttcgg attgattgca gcaacagtca
    37081 ttgcttcatt tgccggattt atgctggaga aggcattcaa aaaagaaaaa gctaagaaac
    37141 taagagaccg cggtaaagat gtcaatgagc cgctcttcaa cttcccggaa caggaggcca
    37201 atcaccatga ctaaactata tttgctactt ttagttgctt gtctgttcat cgtggcatat
    37261 atcccgcgct tgttcccgat gctttatttt actcatcgaa aagtgccatc ctggttcagt
    37321 gactggatga aatatgtacc ggtggcatta tttgcggcgt tagcttttaa agatgttttc
    37381 attactcatg aacatctgga tattgcttgg aatatcaaaa tagctgcaat gattctagta
    37441 gcaggcgtgg cttacaaaac acgttcgatg gcgttgtcag ttttgacagg acttgcgtct
    37501 gtctttttgc tgtctatgct ttaaactgtt ttattttatt aaaaacagag attgcgacat
    37561 aagttgttca aactgatcta tccgaactgc atttgcaaca gtcaattctg gatgcaagtt
    37621 tattcgtagg gataaaaaag attagtgttg gcttatgatg caagctcttt tttattttag
    37681 gaattagaga gggatttgtc cttctatgat tttttgctat aatagggaag atgtttattt
    37741 tatgaaagaa tagttgaggg aatttaaatg aataaaaaat tgatgcaagg aacattttgg
    37801 ctaacctttg ctaatcttct atgtaaagtt ttaggggtag tctatttgat tccctggcta
    37861 agtatgatgg ggaacaatca agatggtatg cttgctacga cattgtataa tgttggctat
    37921 cttccttatg gattgttcct gatgcttggt acagtcggat ttccaaatgc gattgcaaaa
    37981 aaagtagctg tagccacaaa agaaggagat aacgctgctt gtcggctgat ttttagaagt
    38041 acgatcaata tcatgtttgt catcggtatc gcctctgctg cactgatgta tttatttgct
    38101 cctttactag ccggaatcag tccgattgct aacgttaata atgggatact agctatcaga
    38161 agtttatgcc catctttgat tgctatccca attcttagtg caatgagagg atatttccaa
    38221 ggaaaaaatg atctgcggcc ttatggaact tctttgatta tcgaacaggc tgtgcgagtg
    38281 atcgttatct tggctggtac ttattacttg cgcgtattga ctgacggaac catattagaa
    38341 gctgtattga tcagtacagt cgcttctttc ttcggtgggt tagctgcgat tatccatatg
    38401 tatatcgtcg gacgcagaaa agacttcttc gaacttaaag attttttgat ttccagccgt
    38461 tatttcgaaa gagaaaaccg atctgcctct gtctctatca ttcgggaaac actaccgttt
    38521 atttttgtcg gctcagtcat tactatcgtt caaatgatcg accaagtaac gatgaaacca
    38581 ctgcttcact ttttccgtcc agagatcgca gatcagcaac tggaattctt attcagccgt
    38641 gcttctgtca atccaaataa attgacattg atcctgatct ccatggtagg aacagtagct
    38701 ataagcagct tgccaatatt aagcacgatg aagaaaaaag atcggataca aatcgaaaaa
    38761 acagtaggag atagtttttc gattgcgtta ttgattcttc ttccttcatt gactggaatg
    38821 tctttattag ctggaccact atacacattg ttttttggct atgatcctga aagtgtcgga
    38881 tatttccaaa tggctttgct agcttctttg tttttctctt tattcacgat cctatctacg
    38941 atgcttcaat ctttgaatca ccatcgtttt gcgatccgat taactgtaga agcaattatc
    39001 ctgaaagtca tcttccaagt aatcggatta ggattagttg gcggatacgg gatgagctta
    39061 tcaaatggct tagcattcgg tattgtcttt gttcgcggct atcactttat gtgcaaagaa
    39121 taccgaatag cacctttagc aaagatcagc aattttttct taaaaacttt ccgctccacc
    39181 ctgattatgc ttgcagtatg ttttgccgtt ttctttttgc tgaatcagcg attgtcaatg
    39241 gaatcaaaaa gctatgcaat gatttattgt gtggctgtag gaagtattgg cggcttagta
    39301 tttttatttt ctcaattcgg aaaaaatggg tttcgaatgt tgaaaaatgt taaacatcat
    39361 agcagaagca aagaataggt taactaatcg ttttttgctg tcacttgaaa aacggcaact
    39421 cgtatgatta cgcttgtcgt ttttctattt tccaatttta gtttcatatc taatcttact
    39481 tatgatatag ttataaggtc tggaaaaatt ctttgaaacg aggaaattaa acacatgaaa
    39541 cttgcagtag ttacagatag ttcggcatat ctgccggaac gtatcaagaa ccatcccgac
    39601 ttattcgtta ttccaatccc tttgattatg gatgggaaaa tttataacga aggaatcgat
    39661 atcgaagcgg atgaatatta tggactttta aaaaacagta aagattttcc tactacttca
    39721 caacctgtcg taggggaagt tttgggttta tatgaggaat tgaaagagaa agggtatgac
    39781 acgattttaa gtatccatct ttcttcaggg atttctggct ttgtcaacac attatttgcg
    39841 atgaaagatg acatcagcgg agtaacggtc attccttatg actctaagat cactagtatg
    39901 ccgatgggac acatggtaga agctgcctta gacttaaatg atgaaggtca gtcccttgat
    39961 gaaatacttg cacatatcga tcgaatccga gataatacct atgcttatct gattgtagat
    40021 gatttgaaca atttagtacg aggaggacgt ttgacaaatg gtgcagcgct gattggcagc
    40081 ttattaaaaa tcaaaccgat cctgacattt tctgaaggga aaattgtttt attcgaaaaa
    40141 atccgttcaa gtaaaaaagc atttgctcga gctgaagaaa ttattggtag aagaaataac
    40201 gaaatacaac cgccagtcaa actttacgtg atacatgcaa ataatttaga agtagcgatt
    40261 gcggagaaag agaaattgca gaaaaaatat ccaaatgcag aaatcgaaat tggtcatttc
    40321 ggaccagtca ttggtacaca tttaggtgaa aaagccatcg gcattgccat ttcagcgcaa
    40381 taattccttt tattcaaaaa acataccaaa aata
//
LOCUS       DAYYFU010000021        37299 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000021 DAYYFU010000000
VERSION     DAYYFU010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.021"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..893
                     /locus_tag="ACHA34_001290"
     CDS             <1..893
                     /locus_tag="ACHA34_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HFM8791943.1"
                     /translation="LTTGEDGKLAIDGLAPGAYQLVETQAPTGYELDATPIEFEIERS
                     QTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKL
                     AIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKI
                     DDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYEL
                     DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFIL
                     SILGVLLVFISIKFRKKRNI"
     gene            complement(1246..2094)
                     /locus_tag="ACHA34_001291"
                     /pseudo
     CDS             complement(1246..2094)
                     /locus_tag="ACHA34_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2247..3359
                     /locus_tag="ACHA34_001292"
     CDS             2247..3359
                     /locus_tag="ACHA34_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8791944.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3437..3730)
                     /locus_tag="ACHA34_001293"
     CDS             complement(3437..3730)
                     /locus_tag="ACHA34_001293"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFM8791945.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3898..4212
                     /locus_tag="ACHA34_001294"
     CDS             3898..4212
                     /locus_tag="ACHA34_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFM8791946.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4233..6212
                     /locus_tag="ACHA34_001295"
     CDS             4233..6212
                     /locus_tag="ACHA34_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8791947.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6225..6551
                     /locus_tag="ACHA34_001296"
     CDS             6225..6551
                     /locus_tag="ACHA34_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFM8791948.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6567..7085
                     /locus_tag="ACHA34_001297"
     CDS             6567..7085
                     /locus_tag="ACHA34_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791949.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7085..8437
                     /gene="celB"
                     /locus_tag="ACHA34_001298"
     CDS             7085..8437
                     /gene="celB"
                     /locus_tag="ACHA34_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8791950.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8521..10026)
                     /locus_tag="ACHA34_001299"
     CDS             complement(8521..10026)
                     /locus_tag="ACHA34_001299"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFM8791951.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10118..10708)
                     /locus_tag="ACHA34_001300"
     CDS             complement(10118..10708)
                     /locus_tag="ACHA34_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8791952.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10887..12533
                     /locus_tag="ACHA34_001301"
     CDS             10887..12533
                     /locus_tag="ACHA34_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFM8791953.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12582..13301
                     /locus_tag="ACHA34_001302"
     CDS             12582..13301
                     /locus_tag="ACHA34_001302"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8791954.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13780..14727
                     /locus_tag="ACHA34_001303"
     CDS             13780..14727
                     /locus_tag="ACHA34_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8791955.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14739..15659
                     /locus_tag="ACHA34_001304"
     CDS             14739..15659
                     /locus_tag="ACHA34_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8791956.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15700..17157
                     /locus_tag="ACHA34_001305"
     CDS             15700..17157
                     /locus_tag="ACHA34_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8791957.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17502..18116
                     /locus_tag="ACHA34_001306"
     CDS             17502..18116
                     /locus_tag="ACHA34_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8791958.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18109..19833
                     /locus_tag="ACHA34_001307"
     CDS             18109..19833
                     /locus_tag="ACHA34_001307"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8791959.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19830..21281
                     /locus_tag="ACHA34_001308"
     CDS             19830..21281
                     /locus_tag="ACHA34_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8791960.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21754..23907)
                     /locus_tag="ACHA34_001309"
     CDS             complement(21754..23907)
                     /locus_tag="ACHA34_001309"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFM8791961.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23909..24955)
                     /locus_tag="ACHA34_001310"
     CDS             complement(23909..24955)
                     /locus_tag="ACHA34_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8791962.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25105..26394
                     /locus_tag="ACHA34_001311"
     CDS             25105..26394
                     /locus_tag="ACHA34_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFM8791963.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26407..29109
                     /locus_tag="ACHA34_001312"
     CDS             26407..29109
                     /locus_tag="ACHA34_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8791964.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29094..29951
                     /locus_tag="ACHA34_001313"
     CDS             29094..29951
                     /locus_tag="ACHA34_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8791965.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30022..30879)
                     /locus_tag="ACHA34_001314"
     CDS             complement(30022..30879)
                     /locus_tag="ACHA34_001314"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8791966.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            31090..33261
                     /locus_tag="ACHA34_001315"
     CDS             31090..33261
                     /locus_tag="ACHA34_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8791967.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33329..33670
                     /locus_tag="ACHA34_001316"
     CDS             33329..33670
                     /locus_tag="ACHA34_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8791968.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33946..35700)
                     /locus_tag="ACHA34_001317"
     CDS             complement(33946..35700)
                     /locus_tag="ACHA34_001317"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8791969.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35711..36547)
                     /locus_tag="ACHA34_001318"
     CDS             complement(35711..36547)
                     /locus_tag="ACHA34_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8791970.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36549..37232)
                     /locus_tag="ACHA34_001319"
     CDS             complement(36549..37232)
                     /locus_tag="ACHA34_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFM8791971.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        12035 a         6560 c         7655 g        11049 t
ORIGIN      
        1 gtctaactac aggagaagat ggaaaactgg caattgacgg tttagcacca ggagcatatc
       61 agctggtaga aactcaagcg ccaacggggt atgaattaga tgcgacaccg atcgagtttg
      121 agatcgaaag aagtcagaca gcggtggtag aattgacgaa agaaaaccga cttacaccag
      181 gcggtgtcgt gttgacaaag atcgatgacc aaagcgggga agtcctgcaa ggagctgttt
      241 ttgagttaca agatgccaat ggaaccgttc tgcagtcagg tctaaccaca ggagcagatg
      301 gaaaactggc gattgacggc ttagcaccag gagtgtatca gctggtagaa acccaagcgc
      361 caacggggta tgaattggat gcgacaccaa tcgagtttga gatcgaaaga agtcagacag
      421 cggtggtaga attgacgaaa gaaaatcgac ttacaccagg cggtgtcgtg ttgacaaaga
      481 tcgatgacca aagcggagaa gtcctgcaag gagctgtttt tgagttacaa gatgccaatg
      541 gaaccgttct gcagtcaggt ctaaccacag gagcagatgg aaaactggcg atagatggct
      601 tagcaccagg agcatatcag ctggtagaaa ctcaagcgcc aacggggtat gaattggatg
      661 cgacaccagt aactttcaag atcgaaaaag aacaagaagt ggcgatcttt ttaacaaaag
      721 agaatagaaa agttgctgat agctcagata taagaggaaa caatccaaca agcagtggaa
      781 ataagcatct tccaaaaact ggagaaacca tcattagcca gtttatattg tcaatattgg
      841 gtgtgctact tgttttcatc agtataaaat ttagaaagaa acgaaatata tgagctaaaa
      901 taaaaaattg aataagcata gtttttaaaa tcaactagta cccatcaatt gaatggaaac
      961 taccgatttt agaaggggta tacaaacaaa tattagtatc ggagtagaga aggttgaacc
     1021 caaccagtaa ataagtaaag acaattttgt gctgcgttga gaagtgaaaa ataaaccttt
     1081 gtagtaatac tagttaataa aaaatataca aaaacactgc gaaaatcatt cttgagtttc
     1141 gcagtgtttt ttgcctaatg actcaagttt attgggtaat actttgattt tctttctaca
     1201 aataatcagc gatcagttgt aattttttct ctagttttaa tggctctttg tcaataagga
     1261 gaaaaacaag tcgggtcttt tgtctcggaa agcttcaacc aactcgtgaa taaaggttac
     1321 gcctccttga aaggcgtgga aaaaccaagt aatcgatcaa tcaccaattt cgcatttggt
     1381 agaacatgtt tggtgagctg aaagtaggtg gcgttcatgt ctgtaactaa gaattctatt
     1441 tctttaggat tagtgcagct aaggaaatag tttgttaatc gaggaatttt ttgtgtaggt
     1501 aaattttctc gattaatttt tctgtctcgc catccgcgga aataaaactc agtttatcct
     1561 caacggaaac gtgagaacga aattcacaac cctcaacact caagacagaa ttcttttgga
     1621 agactttggt aaatagcttc tcaactcttt caatgtgcga ataacggttg tcaaagatac
     1681 ctaacaactt ttcgaaataa aaaataacga cactttttca gtcagtaaag aagtgatttt
     1741 atgtctgaca tgatttgaaa tcgagtgcct tggacggata aaataacttt gagcgttcca
     1801 atggcttcga cagtttttac aggtatagcg ctgctttttt aggcgtataa ccattggcat
     1861 atgattatat tattcaaagt ggacaatggt ttccttcttc ttgtttttca ccaccattaa
     1921 tttcctattt ccatctacca cagcagaacc acaatttcta cagacacatg gagcagggaa
     1981 gagaactgca gcaacgatca atgttttttt cttctgtaag gtttcgtaag agacctctgt
     2041 aatcatcaaa tttttgtcta ttgatctcag aatttttttg atagaaccat tcataaagca
     2101 tatcgttctc tcagttgttt attttgtggt gatttaatca tacaagaaaa cgatatgttt
     2161 ttttataaaa acatgaatag gaaaaatagt tgacaagaaa atgacaacgc catatgatgt
     2221 tccatagtat ttacaacctg tcatagatga ctgtaaaaaa taaaagtaaa aagaaaggac
     2281 gacaacaggt ggatttttat cagtctctac aactagatcc gtttatttta aaacaaaaaa
     2341 tacgagaagc agcttctaaa aaagaaaagt gcatgtatat ttgttcattg tttttacgat
     2401 cattattcat cgtattgttt gccatttgtt ttatcattat aattacaaca ttatttgaat
     2461 cgaagcataa accatacgct gtggttttat tctgtatgtt gatgagtatt cgttttgttg
     2521 actttggtta taagatttcc cattcgatca taggtttagc aatcgtcatg ttctcactat
     2581 tagtagcacc gtatgtccag ctgatcaaat ggtcagttat gggcatgttg attcatttta
     2641 ttttactaag cagcatatta atggcaaccg ctagtgatcc caaaatgggg aatgcttctt
     2701 tgtacggctt ttcatatctc tttattgttt attcgttacc taaagattcg ttgaacaaga
     2761 atttctttac acaaacaagt agcttattat ttttgttttt ttgttggttt tcagttctcc
     2821 tttacagaaa acatcgagaa aagaacaaag acaaaagctt gtttaaagag atttttttga
     2881 aaggcatgta ttcgcaggaa aaaatttgga tgctcatcta tgcatttggc attagtcttt
     2941 tgatcgttgc aggagagtat gtcccttttc agcgattgat gtgggcaggg tttgcatttt
     3001 cttctattgt atctagctat ggattgatgt cgattggttt caaagaacga gcagtagacc
     3061 gaatcattgg ctcattgatt ggatgtgttt tatttattgg tatcagccaa tttattcctt
     3121 ttaactgggt cggtatatta ggtggtctag ctttaggtgt ttgttctacc tatagatata
     3181 aaacagtttt taattctttt ggtgctttgg caatcactgc cagtttattc ggggttccag
     3241 gagcagtaac gatccgcatt tttgagaata tccttggggt atgtttagga atcatgtata
     3301 ttggggtgac tgaaatactt atccgaaaaa taagagaaaa acacggtttg aatcattaga
     3361 tgagatcgaa taaaaaataa ggaaaaagtg aagatacaca tggatgataa attgctatca
     3421 ttccatgtgt atctttttac ggtaaatcag tgaaacgttc gactttcatt tttaatttat
     3481 actttttacg gagttcgttt atttgagcca tcatttcgga tttgtgatgc ttttcgattg
     3541 cttcttcatc tttccaacgg tcgatcagta aaagagattc tggatcttct agtggaaagt
     3601 aatagtcata tttctcgttt ccttcttctg cacgaatcgc tttgacaatg tttctttcga
     3661 ccatttcttc tgcaaatttt ctggcttgtc catttgtact tgtatacaaa atattcactg
     3721 tgatactcac taaatcccct cctgaaaaaa ttctatcact agtatatcac ggtgccagaa
     3781 attattattc aagatatgat acacattttt tccgcaatgc tacggaaata tatattttga
     3841 atagaaaacg cttacaagat atgcttggtt tataaaaata aaaggagtga ttgatttatg
     3901 aaaaaagcat tgattatttg tgcggcagga atgtcatcct cgatgatggc ctcaaagaca
     3961 acggatttct ttaagcaaaa aagagaagag gtactcgtag atgcagtttc agctacagaa
     4021 ggcgataata tgattaagaa tagtgatttt gatttatttc tgatcagccc gcaaacgacg
     4081 atgtttttgg ataaattcaa aaaattagga gaatctgttg gaaagcctgt tgttagtatc
     4141 cctttccaag cgtatgtccc aatccctaca ggcatccaga agttagcaga ggtgatcgaa
     4201 gagaacatat gatggaatag gagtggatga taatgaaaca gaaggaaaaa ttgctgttga
     4261 gatatttgat tgatcacaag aaggagtatg tcaccagtca acgtttagct tctgaattgt
     4321 tattatctga tcggacaatc cgtaattact tgcagcggat caaagagtta gtagaaaaaa
     4381 acggaggtaa gatcattgct aaaccaggtt atggctacca actgcatatt ttacaaagac
     4441 tgacatttga cttgtttttg agtcggcaag aaatagtacc tggatatgct agagaaccgc
     4501 aaggatttta tgagtccgaa gatcgacaaa agtatatatt gaaccaattg cttttggaag
     4561 atgccattct ttttatggat gatttggcag aagagcttta tatcagtcgt tccagtttga
     4621 caaaggacat gcaagaaatc aaggagcgat tgattccata ttcactaaaa atcgtttcta
     4681 agcatggtca agggacatgg atcgaagggg aagaaagaaa tcgacggcat tttatcatgg
     4741 atactttttt tggaaaccat tatggaaatt cattgaaaga atatttaggg caaagccgtt
     4801 ttttcccaga catcagtttt gaagagctag tcattatcat tctcgatgaa acaagagaag
     4861 ctaagttaaa agtttcggat tttatcatac agaatctgac tttacacttg gctttgggga
     4921 tcaaacggct ccgagaaggg tttgaaatca aggaattagg catcgaacaa gaagtttcta
     4981 agcgagtaga gtatcaagtg gcgcagcgga tcgttcaacg tattgaggta atcgcaggtg
     5041 tatcgtttcc cgaagaagaa attgcttatt tgacattaca tctcatggct aagtcgaatc
     5101 acaaagaaaa tcaaaatgat caggaactat caaaggaatt agcgaaagtt ctgaaaagat
     5161 tgtctcagga atttcatttt tcattagtag aggatctcca gcttttcaat ggactactcg
     5221 atcatttgtc accgatgatg attcgattga gccgaggtat tcgcattgag aacccattga
     5281 ctgaggagat aaagaaagaa aatcccaaag tctttgacgc agtaaaaagg catttcagca
     5341 acatgccggc tttgaaaaac tataccatca atgaagatga atgggcctat ttggctttgc
     5401 atctcatggc ggcattggaa aaagaaagag ctgcgcataa actacatgca ttgattatct
     5461 gtgcaacggg ttacggcagt gcccaactat taaagaatcg cgtagtcagt gaattcggta
     5521 aaaatattac agtggtaagt gtaaaaggct attatgagat caatgaaaca actttaaaag
     5581 gaatcgacct gattatttct tctattgatc tttcgacaat gtttttcaaa attccagttt
     5641 tacatgtcag tatttttttg aatgaagatg atgttcgtaa aatacgaaag gtagttggag
     5701 aatctatacc ctatcattcc tcagatagac cgataccagc attttctcta cagcaaaaaa
     5761 agcagattta caccgaacag ttgtctgaaa aatttttcaa gacttatacg tacgaaccga
     5821 aaaaggaaga aattcttcat gatttgctgc aaaagctgag cataaatgaa gaagaaggat
     5881 atataagaga aatgcgaaag cagatccagc aacgtgaaaa tatgggacaa atcatattca
     5941 gtgatacagt ggcagtccca catcccgctg ttccagttgg aatcgaaaca aaggtagccg
     6001 ttgcattgat tccttctggg atgcagtggg atcaatatca agaaattcat tttgtttttt
     6061 tagtttcgcc ttcttatgtc gaaaatgaag gaattacggt cgtgactaaa gcaatcgtta
     6121 ggttagttga ccagctaaaa gtacagcagg aaatcttagc tgaacctatt tttgagaatt
     6181 tcagcagtaa atttatcaaa ctgatcgaat aaggaggaaa acagatggct gaaaaaataa
     6241 acagtgaaac actacaagtg acttcttttg atatcatttt ccatagcgga aatgcacgga
     6301 cactgataca tgaagcattt cgctcgatgc gtaaagagat gtttgaagaa gtagaagaga
     6361 agttgcgaca agcaaatgaa gagattcttc atgcgcatca gtcacaaact caacttctga
     6421 aagaatatgc cagcgggaca aaaatagaaa tggaaattat catggtccat gcacaagatc
     6481 atctgatgac gacgatgaca ttattggaag ttgcacagga aatgaattat ttgtatcaaa
     6541 aaaataaata gaaaggaagg atcattgtgc aggatacagt aaaaaggaaa atcgaattag
     6601 aaaaaaagca acttagtttg aaaaacatgt atttcaatcg ttatctaatc gtacgttacc
     6661 tgactgcctt tttctttttc atgaacatgc aatggatgat tctgcttcta tcagctaaaa
     6721 gtttaggaag tagtttaccg atgattctga ttttagctat tttgcctgca gtaggagaac
     6781 aagtaaagct ttatcgaaaa caccaaacga acgttccatg gacaaagtgg tattttttgt
     6841 tccagagtgt ctttaattta ttagtgattc cagtgttgtt tacctcagga tttacattct
     6901 tgtatccttt tatggcaaat aacaatggag gacagttatt tgttttcatt cttgttgtaa
     6961 gcggtatttt tgccagtgca tttatacagt atcgtttaaa gaaaatttca ctcaaccaag
     7021 accaacagta tatccgtatt aaacaatatg aaaaagcgtt atatttagga aaggagaata
     7081 actaatgttt gattttttgc agaaatacct gatgggacct atggggaaaa ttgcgcaact
     7141 taaaatcgtt cgggctgtca tggcagcggg gatggcttct attcctttca cgattgtagg
     7201 ctcgatgttt ttggttttaa atattttgcc attaccgttc ccatttttag aaggattttt
     7261 taatgcaact tttttcaaag ttagtgactt gtacatgatc gtcaatacaa tgacgatggg
     7321 gattttatct atttactttg ccattgtgtt tgcttatgaa ttaacgtcaa ttgaacgaga
     7381 tgaacaaaaa ttaaatgtta atccgctcac gggggcatta ctatctgttt ttgcattctt
     7441 tatgtgtatc cctgaattga tcatgactga tggtaaaatc agcttgattg cttctatcaa
     7501 tgagacagaa actgtagtaa atggtattag gatgggctct tttgtcgaac gtttaggaac
     7561 ttcaggaatc tttacagcta ttattatgtc gattattgcg gtcgaattgt attgtatgtg
     7621 tgtcaagaga aattgggtca taaaaatgcc agatgttgtt ccgccaggag tttctcgttc
     7681 tttcactgct ctgatcccaa catttgtcat tgcgtttgtg gtgatgatca tcaatggatt
     7741 attagttgca ctaggaacgg atatcttcaa aatgattgct atcccatttg ggtttgttac
     7801 gcagttgacg aatacatggc ttggtgtcat ggtgatttat ttcttgatcc atgcattgtg
     7861 gatcgtaggg atccatggag cgaatatcat cacttctttc ttgaccccga tcgtattagc
     7921 taatatggca gctaacgcac aaggcgcaaa ttatccgttg gcaggagaat tcaacaattc
     7981 ttatgtaacc gttggtggat caggtgcgac tttaggattg atcatcttta ttgcattcct
     8041 cgcgaaatct gatcagctga aagtgttagg aaaagcctca cttgtaccag gattattcaa
     8101 tatcaatgaa ccaatcattt tcgggatgcc aatcgtctac aatccctatt tagcggttcc
     8161 gtttttctta gctccgatgg cttctgctac actcgcttat tttgccattc ggctggaaat
     8221 cgtcaaaccg atgcttgcac agatgccgtg gccttcacca gtcgggatcg gagcatttgt
     8281 tggtagtggt ggtgattgga aagctgctgt gttagcagtc atttgtgcaa tcatcgcatt
     8341 cattatctgg ttcccattta tcaaattcta tgataataaa ttattaaatg aagaaagaga
     8401 aaaggcagca gaattagcta gtgaaaagtt aaaatgattt ctaagaaaaa aggaactgtg
     8461 acaaaaaatc acagtccctt tttttcttag aaatgggaaa ccactagtat ataaatacca
     8521 ttattcttca atcaaccgct tcacgattgc ataatcccct tcataaggtt catctacacc
     8581 attgattttt tgatagggat ccacgccttt tcccgcaata acgactgaat cttgcggagc
     8641 gctttctgct aaagcgcgtc gaatcgcttc aggacgatcc atttcataat gaacgatcaa
     8701 atctggatta gtaattgctt cattgatttc ttccgcgatt tttttcggat cttcaaatgc
     8761 tgggtcatct gccgtcaaaa aggcgatgtc agctgtttct gatagcactt ttccaaaatc
     8821 atgtcgacgg gagattgctt tgtttcctgg gctgcctaat acaacaatta cacggccatc
     8881 tggatgttca tttttggcaa atttcagcaa tgttttcaag cttagataat tatgtgcata
     8941 gtccacatag acatgcgctc cattggattg gatcaattga tccattcggc ctggtacacg
     9001 agcttctttt aatccgtctt gtgcatcttg actggaggca cctgttagtg ctgcggcaat
     9061 gatcgcactg gctgcatttt cttggttgaa tcctccagcc agcaaaatat cataagcagt
     9121 atcgcagata gctaagcgat cttcatgaga agataaagta aaaccgtgag tacctttatc
     9181 tgaacggatc acttgataat tgctttcttc cgaacgacca taagtgatcg ttggaatctg
     9241 gaatagatcg gctgtttccc taagtaaatc aaaatagtca ctttcatgat ttaaaacaac
     9301 tgtttttgaa ttcaagatca attgtcgctt gcaatagaaa tagtcatcaa atgtcggatg
     9361 ttcgatcgga ctgatatggt caggcgagat attcaagaag attccgacat ctaaagtcaa
     9421 gccgtatacg cgttgtgttt tgtaagcttg tgaagaaact tccatcacta gatgagtcat
     9481 accattttca actgcttctg acatcatacg atataaatct aatgactcag gcgttgtcaa
     9541 atgggatttg aaatatgttt tgccatctaa cgttgtattc atagtagaaa gcaatgctgt
     9601 ttttttattt gttgtgtgat ccaagattgc ttttgtaaag tacgcagcag ttgttttacc
     9661 ttttgttcca gtgaatccga tcactttcaa tttattttgc ggatagtcgt aaaaagccat
     9721 agcaagaagc gccattgctt tacggatatc cgttacgatg attcctactg ctgaattttc
     9781 gtatggttgt tctgatacat aatattgaat accctgttcg attgctgaat ccaaatattc
     9841 ttctttgaaa ttcattcctt tacagaaaaa caaagtagaa gcatcagctt ttcttgaatc
     9901 ataacttaac tgaagaaatt cagctttttc tggtacatca agatgccagc ctcttgttga
     9961 aacaaattct tttaatagat tttcttttaa tagtaaatga cggatgtctt ctaaagagaa
    10021 gctcattgat tgatccctcg ctttattcaa tttcttggtt tattatagca cactcttttt
    10081 aatatatcgt ttgtttccgc tatgacaaaa tccagcttta ttcaaatttt cttgacacag
    10141 gggacaagcg gatgagtatt ttcataaaca atgaactgac tgtaatagat aggagaacga
    10201 atagtgtctc caacaatcca aaaacataat aattcaaaaa gacgataaga ctattggtaa
    10261 gtaaaagtcc ttgcggcatt ggaagccgaa aaaatttgtg gaaaataatc ggcggtatcg
    10321 ttataccgcc atttgaggcc ccgactgtgt acaatgtgta aaccccaaga ctgaacaaaa
    10381 gacttcctga aatcagtgaa aagaaatgtg aggagatcat ttccactttt ggaacaatct
    10441 ctaaaacaac aggaaaaagc agacttcccg ctacagtctt aaaaaatact gattttccta
    10501 ataggacagc ggctagaagc agcatcataa gattgatgct ccaaacagta actgagcgtt
    10561 ggatagaaaa ggccgtttca actaaatgtc cgatggcgcc cactccggcg gaagctatat
    10621 cgtgagggag caagaaccaa ttcaatccaa ctgtgataaa gaaaagacct aaaaatataa
    10681 cgggtagttt ttttatttct tgtgtcattg atatctccta taaatgttag ccataaaagg
    10741 ctgtatattt ctttacttta ccaaaaaaac gaaaccgaat tcaaaaaaac aattgttctt
    10801 ataaaagtta tataaaatcg attgccagtt tctgttttac caattaaaat gatagtaatg
    10861 ttttgtagaa agttggaatc tgaccgatgg ataatcaaag aaaaccagca ttagatcaat
    10921 taacaccgca agaaaaaatg gcacgaggct ctgcttggat gaccgccagt aatatcattt
    10981 caagattgct aggcgctgtc tatatcattc cctggtatgc ttggatgggc gaaaatgcta
    11041 aggctgccaa tggattattc aatatgggat acaatatata tgcattgttt ctactgatct
    11101 ctacagcagg aatacctgct gcgattgcaa aacaaactgc acgctataat tcattgaatg
    11161 aatatggcac ttcacgacga ttatttatcc gtgcattaca gatgatgggc gggttaggcc
    11221 tgttgtttgc tttgttcatg tacatcgcct ctccttggct agctcatgct tctggtggcg
    11281 gagaagaact gattccaacg atgcgttctt tgagtatcgc tgtattggtt tttccttgta
    11341 tgagtgtgat acgagggtat ttgcaaggca atcaagaaat gatgccttat gcgctgtcgc
    11401 agattgtaga acaggtggca cgtatttttt atatgctatt ggctacattc atcatcatga
    11461 aagtcatgga tggtgactat gtgacggcgg tcacgcaatc aactttcgcg gcatttatcg
    11521 gtatgctagc aagtattctt gtccttttgt attttttgaa gaaacatcaa gcgtatacat
    11581 cggcgtttat tcactacagc gaaaacaaaa taaatattgc aacgaaggaa ttgttgttag
    11641 ataccgttaa agaagcgatt ccgtttatta ttgtgggttc aggggtaacg atcttcaaat
    11701 tagtcgatca gttcactttt atgaagatca tgtctgcatc caccgagtat tccaatgcac
    11761 agttactgga tcttttttcg atttttagtg ccaatccaga taagttgacg atggtagtta
    11821 ttgcattggc aacatcgatc gcgtctactg ggttaccgtt gattacagaa gctgttacgt
    11881 tgaaagatcg agtaggtctg gcaaaattga caagcggaaa tttacagttg ttttcatttt
    11941 ttatgtttcc ggcgacattt ggtgttatgt tacttgccta tccgctaaat acattatttt
    12001 atacaccgga ttctctagga agtaatgtac tgatccaagc aagttttgta ggactcttcc
    12061 taggactata tatgctagtt tccaacatgc tgcaaggaat gtttgaaaac aaggcggcaa
    12121 ttaattattt ggttattggg tttctagtga aactccttct tcaataccca gccattcgtt
    12181 tatttgaagt atacggccct ttattggcga caatgatcgg atttgctgtg tcttgtacct
    12241 tgatcttaaa gagaatccat gaagtagccc gctttaaacc aagttttgtc tggcgcagga
    12301 cattgctgat ctttattctt accttgatta tgcttttggc ttcgtggatc acaaagatga
    12361 tttttggtgc ttttttaaac gaagatagca aattccagtc tctattgttg attcttgtag
    12421 tagcaggtat aggggcttta gtgtatggtt atttagcttt gaaaatccgc ttggcagaaa
    12481 gactactagg accaggaata gcgcgtttac gacataaatt aaaaataaaa taagtgaatc
    12541 agaagagcta gattgatgct cttttgaaat gggggaaacg catgcgttta gataagtttt
    12601 tagcagatat gggacttggc agccgcaaag aagtcaaagg attaatcaaa aaaggaacga
    12661 tcgaagtcaa tggacaagtg gtgaaaagcg ataaagcgca agtaaatgaa ttcgaagatg
    12721 cggttactta tttaggagaa atcattacgt atcaaaaaga tttttactac attttgcata
    12781 aacctgcagg agtcatttct gctactcagg ataatcatga tcaaaccgta atcgatttat
    12841 tggaagacca ggattatcgg gaagatcttt ttccagtcgg ccgattagat aaagacacag
    12901 agggcctctt gattttaacg aatgacgggg tgtttgccca tcagttacta tcacctaaaa
    12961 aacatgtgga gaaagaatat ctagctgagg ttagcggtat aatgacagaa gaagatatcc
    13021 gtttatttgc agaaggactg attattgatg gcgatgagca gacattgccg gcaaaactcc
    13081 aaattgatca agtagatcaa gagacagaaa cttcaattat ccgtctgatt ttgcatgaag
    13141 gaaagtttca ccaagttaag cgaatggtca aagcagtagg aaaagaagtc ttatatttaa
    13201 aaagaatccg tatgggaaaa ttttggctgc ctaaagattt gaataaaggg caatatcgac
    13261 ccatgaccaa agaagagcta gaacaagtga gagaaaaata attgaatgga tagctaaaag
    13321 aataagcaga gtagttgtag cgccatctga aaagcagact tgtccgtctg actttcggat
    13381 ggcttttttg tgaaatttcg gcttatttcc gtagaagcta cttctggaac accgtttgtt
    13441 cggaataagg gactgtgaca agacttttgt tacagtcccc attgttcctt gtgactcaga
    13501 gttagatagt aaggacacga tctccctaat actcaggcca aaacgctgaa ttcacaacct
    13561 ctgataaacg gtgttcaatc agctgaccct cggtattagc tcaaaaaacg gcaaaatatg
    13621 aggaactatt ttcccgtttt tttctctaat actcaggcca aaacgctgat ttcacaacct
    13681 ctcctatact ttgtttcatt ttccctatac tttgtttatt tattctcatt attgaaagcg
    13741 ctataattac gttgcgaatg aatcgaaagg ggtttttaga tgaaaaaacg aaaggggttc
    13801 aatcattttt ttatgaatct atggcgttat aaagcacttg tcctgatggc tgtaccgggt
    13861 atgctttgga tgattttttt cttttacata cctgtaatgg cgaatgtcgt agcattcaaa
    13921 aattttcata tttcagcaga tgggttcttg gctagcttga gggaaagtcc ttgggtgggt
    13981 tgggaaaatt tcaaattttt attttcttcg gatcaagcat ttctcatcac taaaaataca
    14041 attttatata atgtagcatt tatccttttg aatctgctga tctcagtcgt atttgcaatc
    14101 attatgagcg agttgagaaa taaacgactc gttaaagttt atcaaacgat gtccttacta
    14161 ccttattttc tatcttgggt cattatcagt tactttgttt acgctttttt aagtcctgac
    14221 aaaggaatat tcaatcagtg gatcactgga catggaggag aagctgtcaa ttggtataat
    14281 gaaccgaaat attggccgtt tatcttggtg ttcattggta catggaaagg gattggttat
    14341 aacagcatta tttactttgc atctgttatg ggaatcgatc cgacttatta tgaagctgct
    14401 atggttgatg gagctagcaa atggcagcag atcaaacatg tgacgatacc acaacttatt
    14461 ccactaatga cgattttaac gattttagcc gtaggaaaca ttttccgagc tgattttggc
    14521 ctgttttata acattcctag gaattccggt gcattatatg atgtgactca agttttggat
    14581 acgtatatct acaatggcct gacatccaca ggagattttg gtatgacggc tgctgcagga
    14641 ctttaccaat cagttgtcgg ctttgtcttg ttgatgatca caaatacgat cgcccgacgt
    14701 tttgatagcg attctgcatt attctaagga ggaaaaaaat gaaaaaaaag aaagtggaga
    14761 gagtccaagt ccgctctttc aataagacgg ctaatttttt ctctaatata atgattgcac
    14821 tctttgctat ttcctgtatt ttgccattta tatttgttat cgccatctct tttaccgatg
    14881 agtccatatt gtcgactcac gggtacagtt tttggccaga aaagttcagt atatttggtt
    14941 atacgtactt gttcgaccag atgcaggata aaattttcca agcgcttttc gtcactgtac
    15001 ttgttactgt tttaggtaca ctgatcaata gcacggctac ttctttatat gcttatgcga
    15061 tttcacggtc aaactttcca ttccgtcgtt tttttaccgt tttttgtttg attacgatgt
    15121 tgttctcacc agggatggtg gccaattatc ttgttatgac aaatcttctt cagttaaaag
    15181 atacgatttg ggcattgatc ttgccaatgg ctgtcagtcc cttcaatatt attgtgatgc
    15241 gcacattctt taaacgttcg gtctctgatt cgattattga atcggcacgg atcgatggag
    15301 ctagcgaact gcgaatattt gttcagatcg ttttaccgtt agctgttcca ggtattgcga
    15361 caatcagttt atttgccgca ttgggttact ggaatgactg gttcaatgct ttactataca
    15421 tccaagatga taagctagtt ccactacagt atcttttgat gaaaatccaa agtaatattc
    15481 aatatctgac acaaaattca ggagcaggaa gccaattgac aggcggatta gcctcagttc
    15541 caggagaatc tgccagaatg gcgatcgtag tcatctcgac gttgcctatt gcgatcagct
    15601 atccattttt ccagaagcat tttgtcaaag gtctaacaat cggcggagta aaagaataaa
    15661 gaaacaaaaa tataaatcat aaaattagga ggaagaaaaa tgaagttatg gaagaaacta
    15721 gcctttacag gtgtttcagc gttgttgttg ggaacattag cagcatgcgg aggttctggt
    15781 tctaaagacc aggcagaagc tagcaattcg gacactttgc agatgtatca aatcggagat
    15841 aaaccagata atttcgatca attgatggaa gtagcaaaca aacgaatcga aaaagagatc
    15901 ggtgtaaaag tcaatatcaa ttacattggc tggggcgatt acgaaaagaa aatgaacgtc
    15961 atcatttctt ccggggaaaa ttatgatatc gcctttgcga acaactatgt ttccaatgcc
    16021 caaaaaggag cctttgctga tttgactgaa ttagcgccta agtacgctga aaaagcttat
    16081 aatgatttag atgaagcata tattaaagga aatctggtca atggaaaatt atatgctttc
    16141 ccagtcaatg gaaatgtctt tgctcaacaa gtacttactt ttaacaaagc tttactagat
    16201 aaatacgatc tttcaattga tggaatccaa tcttacgctg atgcagaaaa agtcttgaag
    16261 gaattccatg aaaaagaacc aaacacggca gcttttgcca ttggacaagg cttcaaagtc
    16321 caagggaact ttgattatcc tttagggaat acgttaccat ttgctattga tctggatgga
    16381 gatgattcga aaatcatcaa ccaatacgat aatgaaacgt atatcggact gttacgtaca
    16441 atgcatagct ggtacaaaca aggactcatt ccaagtgacg ctgcaacgag caataaggat
    16501 tatccactag aaggaaatac ttggctaatg cgtggagaaa cacaaggacc gtatgattat
    16561 ggcgatacaa ttttgacaaa tgcagcaaaa caagaacttg tctcaaaagc aattactgtt
    16621 ccactaaaat ctacagctca agcacaaatg gcaaattttg tcgtatcaaa tacttcaaaa
    16681 aataaagaaa aatcagtaga acttctaggg ctgcttaaca gtgatccaga attattgaat
    16741 gggcttgttt gggggatcga gggagaagca tgggaaaaag tcgatggttc agatgataaa
    16801 atcaaattgc tagatggtta tcagccaaat acacatatgt ctgcttggaa tacaggaaac
    16861 aacaagattc tatatacaca agagtctatt acggatgaca tgattgccaa acgtgatcaa
    16921 tcgattgctg atgcagaaac ttcaccgatt ttaggattca gcttcaacac tgacagtgtc
    16981 aaaacagaat taagtaatat ttctaatgtc atgaatcaat atttagatgg attgaataca
    17041 gggactgttg atccagatga aacattaccg aaattgaaag acgctttgga caaggctggc
    17101 tatgataagg tattgaaaga aatgcaaaaa caatatgatg agttccgaca agaataataa
    17161 tgaataaaga caaaggggtt gtgacaaaag acgtgtcaca acctcttgtt ccgaataaac
    17221 ggtgttcaaa agccagtttc tcggtattaa gctaaatata tgtaaaaatg tggaaagcca
    17281 tattcacaaa atttttctta ctactcgaaa ctaagcggct atttcacaaa ctcttggtaa
    17341 acggtgttca atcagctaac cctcggtatt cgctcacaaa acgacaaaat atgaggaact
    17401 atttttccgt ttgtttctct aataatcagg tcaaggtgct gattgcccaa cctttttttg
    17461 tataatgaga aagtataaac ttaatgtttg gaggagaaga tatgttaggg aaggttttgg
    17521 aagagttatt tatccggatc tgggtcatta taaaactaac cttgtatttc tggatatata
    17581 catttgccgg cgggatcatc tttggattag gtgcggcatg gaaaacagtc aatgaactat
    17641 tttatttgta cggattcgag tataaagaaa tcacaataaa aagaggctgg aatatatata
    17701 aaagaaattt tttaagaggc aatctgctat ttagtttatt tttatctggt actgctttat
    17761 tgagttacaa tttatattta tctgttcaaa tcaaagggct cattttttta gctattgatt
    17821 ttattttact ttttgccttg ctctgcctat ttgctactta tcaatacagt atgattttag
    17881 atagtgaata tacgatctct gttcctaatc tgctaaagct aagttttatt tcggtttttt
    17941 ccagcttttc gtcttttcta aaaataatta ttggtagcgg tataatttta ggcgtgactt
    18001 ggcaattcaa aggattgatc ctgtttggtg tcattggtct tttgacggtt tggaatggta
    18061 cgatgacaac acattggcgt gaagaattag ataaacagtt ggaaagttat gaataagttt
    18121 cggattctat tttcaaaagg aaccttacta aatcgtctgt tgcggaaata cgcggttatc
    18181 atgatcggtg taacgatgac tgctaccgtt atttttagta tccatacttg ggaacaaaat
    18241 caaaaacagg cagaaaacat gacaagtgat gccgttcaaa gcacaagcag aatgctgaat
    18301 gataaaacaa ccttgagtag gatcatcaaa aaccagcttg tagggaattc cgaaaagatc
    18361 gaaaatgtga cgacctactt aacaaaaccc atagatcagt atctgatgta tgtatacgaa
    18421 cagcaaaaca gtaccgatga attagtttct ttcccaaatc aaatcaaaga tctctacgca
    18481 aattatgaag aactaagtgc gatatatatc gtgttgaatc aactaccgga gtattatgaa
    18541 tcttctagag aaaacaaagg tggggaaata aaaaagggga caccaaaatt actagacgcc
    18601 ttttatatca agttgcctat cacacaagga ggagctgagt caggaagtat ttttattggt
    18661 tttcaaaaga aagaactaga tatgatttta gaaaatctaa cgtcattcaa tggactctct
    18721 ttatacatga tttctggaac gacaaatcga ctatacacat tccatgataa taaaatagct
    18781 aaagaaacgt ttgaaagaca agaaatgatc atcacagaga gtctgaagaa aaattcacaa
    18841 ttgccgattg acgatctgga aaaaaacaat tttgttcaac accaagaact gtctgatgat
    18901 tatcgtatat tagctgtact agataaaaaa agtgtaaaaa tcgaaacggg aaaaaatctt
    18961 gccccgctga tcattggtat cattatttta gacaatatac tgcttatctt tctttataaa
    19021 acattcaaac gatattcaca gcaagtatct atcgtcatgc aggccatgga tcaaacagca
    19081 gcagggaatt tagagacgcg gatcgacata acaaacacgg aatatgaact gagggaactt
    19141 tcaatcggta tcaacgaaat gttggatagc atcaatcagt ttgtagaaga catctacaaa
    19201 ttagaaatca aacagcaaga cgcacatatg cgggcgcttc aagcacagat caatccacac
    19261 tttttatata atacattaga atatatccga atgtatgcaa tcagcgaagg aagtgaagaa
    19321 ttagcagatg ttgtgtatgc gttttccgct ttgcttcgaa acaatacgaa tcaagagaaa
    19381 acgatcactt taaaagaaga actggacttt tgcgaaaaat atgtctattt gtatcaaatg
    19441 cgttatccta atcgagtagc ctatcatttt atgatcgatc ctgatttgga aaagatcgaa
    19501 gtacctaaat ttgtgatcca gccattagtt gaaaattatt ttaaacacgg tattgatttt
    19561 actcgattcg ataatgcgct aagtgtcaaa gtcttgcagg aaggcaaacg ggtaaggatc
    19621 atcatcaaag ataatggaaa agggatgact gaaaaacggc taaaacagat tgaagaaaaa
    19681 ctgtctcatc ctaaagtgga acttcacggt tctatcggtc tgcaaaacgt gaatgaacgt
    19741 ttacgtgcca gcttcggcag cagttattat atgtcgcttg agaataatga gacaggtgga
    19801 ctgaccgttt ctattacatt caaggaggga tgagcgtgta tcaagtgtta ttggtagatg
    19861 atgaatacat gattttgaat gggttaaaaa agataattga ttggcaaagt ttaggctttc
    19921 aaatagtagc aacagcagag aatgccaaag aaggactagc tgtacttgaa cagcgacaga
    19981 ttgatttagt agtaacggat gtgacaatgc cagaaataaa tggactggaa tttattgaag
    20041 cggctcaaaa agaacgtcat aattttgaat ttatgatttt atcaggatac caagaattcg
    20101 attatttgaa aggaggtatg caattaggcg ccgtgaatta tttgatgaaa cccgtgaata
    20161 agttcgaatt ggttgaaagc ctcaagaaaa tcaaaacacg gttagatcag caaaatgaac
    20221 aaaaaaatca acaagaaatc tatcaagaac tgttattcag tcaatggttg aatgaagaat
    20281 tggatgaaat aagtgaagaa gaaataatag gacaaataag tgagaagcag cataggatag
    20341 tattgattca actggcaaga actcatgata ctcttatcaa tagatggttg caagaacggc
    20401 aacaaatctt ttactatcaa cgaaattacg gagaccgtgt attgatcacg ttgttgttgg
    20461 aggcagatga agttgatggc ttctgttgtt ttgttcaaga aaactttcct gatcaggaat
    20521 ggctgatcag tatcggggaa gagacagatt ctttggacaa aatccctgaa acattccaac
    20581 aagcgaaaga caatctacag ttgcatcaat tttatggaga taaagacgag catgtttttt
    20641 acgcagtaca agcagaaaca aaagatcaaa ccatcgattt ttcaagtttc agacgcgtgc
    20701 tgcaaaataa acaattggaa gtcgctcaaa aaatgatcac tgattttttt gaacagtttc
    20761 agttagcagc catgatgccg gaagatatac gatattccag ttttttactg tttatggaga
    20821 ttcaacggga attgattgat ctggaagatg aggaatattt acaaggaatc gaaaaaatcc
    20881 atcaggcaaa aacggtgcaa gaacttcagc agctacttct gtcattcatc aaaaagcatc
    20941 agagacagaa aaagtacagt gacaatgtgg aaaaagtaat cgtaatcctg caccagcatt
    21001 atcaagaacc acttacactc aaagaagtgt cagaaaacct gcatttaaat gtcatgtatc
    21061 taggacagct gttcaaaaaa gaaacgaaaa aaagtttttc agcttatttg aaccatttac
    21121 gtatggaaaa agcgaaacaa ctgcttttgc acagtaatca aaacatcaat gagatagcaa
    21181 gtgagattgg ctacaacaac accacgtatt tctctaaatt attcaagaaa atcgtcggca
    21241 gatcccctaa agagtaccga gaaaacagtg gagaatcata aaagaaaaag ccaatgtaat
    21301 gaaataactt ataaaaaatt gtctagaaat ctaattttgg gctgttctta ttacttgagg
    21361 caaggcagct ttttcatgac ctctcgataa acggagttca aaagccagtt tctcggtatt
    21421 aagccaaatt tatgcaaaaa tgtggaaagc cattttcaca aaatttttct taatactcga
    21481 aactaaacag ctttttcacg acctctccac taaacggtgt tcaatcagct gaccctcggt
    21541 attagctcaa aaaatggcaa aatatgagga actattttgc catttgtctc tctaatactc
    21601 aggtcagaac gctgatttca caacctctca ataaacggtg ttcaaaagct tgcatctgcg
    21661 gtaataagac caaccaaacg aaaaatatga ggatctattt ttgcttggct gttcttatta
    21721 cttgatgcag gacagctttt tcacaacctc ttgttaatta tagtttgata aggaaaaagg
    21781 caaatctttt gcttgatatt tcttagggtg agggatgatc cctaattggt aagagatcgt
    21841 gccgcctttc agcaaatctt gatgggtaaa atataatttc gtatattctc tgtccatccg
    21901 tttgatttta tggataaatt gttgctgcgg ccgattaggc gttgcttcaa tcgtcaattc
    21961 ctttccattt gacaaaagca aaacagcctt gtcaattaat ggcataccga taacatattc
    22021 accggttcca ggcgtgacag gataaaatcc cagactattg aagatatacc aagcggccat
    22081 acttccattg tcctcatctc cagggtaacc cattggagaa tcgtcgaagg tctgagtcat
    22141 caactgttta agcagtggct gtgccatttc aggttgtcct atataactga acaagtaagg
    22201 atagtgaaaa cttggctggt ttgaaatagc taattgtcca aattctatcg cggccatctc
    22261 actcatttcg tggatctcaa aaccatatcc tcctgggtca aacgttgggg gctgattgca
    22321 cagctcaatg agtttttctt tatatctctc actgccacca tattttccga tcagtcctga
    22381 aaaatcgtga taaacagcaa agctgctttg ccaagcactt ccttctgcat aatcttgtcc
    22441 ccaacgatta gctgaaaatg gttctctgaa cttgccatct ttatcttttg aacgcataaa
    22501 tcctgactca tagtcaaaga tattttgata gttcaatgat tgctgctgat atttcttcgt
    22561 catttcctct tcttctagta ccttggcaac ttggctaata caaaaatcgc tataagcata
    22621 gtctaatgtg tgattgactg attcgtggta gtgattagga acatagccat atgtcaagta
    22681 atcttctgtt ccttggcgtc cataatttgg atgtttgctt tgaaccgtag cacctttttc
    22741 cattgccgtt agaaattcag gcatcaagtc tcgtcggata tcttttacag cagcatctgc
    22801 aatgaccgca tcgatcaatg tcccaggcat aagtccacgt tcatcaggag acagccattt
    22861 tggcaaatag ccactttctc tatagctgtt caaaaaacct tccagcattt cttcgtattt
    22921 ttctacagca atcaaagaat aaagaggata aaccgtttta tacgtatccc aaaacccatt
    22981 atttgtatag agaacaccag gttttataga tcgactagtc gtatcatagt ggattttttg
    23041 cccactagga tcaatttcat aaaatgtttg tggaaataaa aacacccgat ataaattatg
    23101 ataaaacgtc cgcaattgtt gttgatcatg gtgtgtgagc tgtatacgat caaaatagga
    23161 ctgccattgt tcttgactgt gagcaaggta agtctctttg ctttggtttt gttctagttc
    23221 caggttataa gctgcttgtt ccgagctgat aaaagatgtc ccaaatcgga tagtttgttt
    23281 ttttgtctgt tcaaaataca acgggatcga gtcgctattt ttgttggaaa cagctaactc
    23341 atcaattgtt aaaggatatt caaatctcaa tataaaataa aaagaaaaat caggatcttc
    23401 tgaaccagca aaatgtgtga tttttccttc cactgtgtaa ggatctataa caaagagctg
    23461 atggcgtccc ggaaaggata aaagtaaacc gctgtcattt ttgctgtaat caatagataa
    23521 gattccgcca tacatgctag gtatcaatgt agaacagatg ccataacgta attgacagat
    23581 cgacagatga gtcgggcaaa atatactttc ttctgggcgg taagaacttt gagcatggaa
    23641 aagggtgttc tcctgtagtt ttccattgat cggcgtaaac agcatatgac tgaaatcacc
    23701 catccaagga cttggttgat gggtaagacg gtagccttga aaaacgcggt catcaggatg
    23761 aaaccaccag ctgccgtttt gatccgtagt ttgcggtgca aaataattca tcccaaaagg
    23821 aacgccagta taaggctggc agttcccatt agagaaattt ggctgattcg ctgttgcatg
    23881 tctagtatct atttgaccga tattcatttt atttctcctt tgttgattgt cttttgatga
    23941 atgaaactgg aaatttacta tcggatggtc gatcttttgt ctctaaccaa gtgaccaaag
    24001 catctcccgc aagcttacct aactgttcaa aatcttgtgc gatggttgtc agtggcgggt
    24061 caacaagagc tgctgcttgg atatcatcaa atccgataat tgaaaaatct tcagggatat
    24121 gataaccatg ctgtctcaag agattcatgg tctggatagc gacaacatcg ttttcacaaa
    24181 caaaagcagt tacttcatac ttagtgatat aagaaagcaa ttcatctaat gtcgcgcttt
    24241 tgtcatccag tgaagtgtgg aaagtcaaac cagctttgtc caatgcttca aggtaaccaa
    24301 ggtaacgctg tcgaacggat tgtggatgag tctgcgtacc aaacaaataa gcgatttttg
    24361 aatgtccctg ttcgatcaaa taagaggcag caagcgcacc gccttggaaa ttatcggaaa
    24421 gaacggtagg aaaaggtaac tcatagattt ttttatctaa tatgacgaca gggaattctt
    24481 tcaatgaaag ctcaaaaagt aagtcaagat gttgttctgt atcaagagca taataaatca
    24541 atccgtcaaa ttcattgatt agctcttttg ctgttttatt ttgtagaaag tcaagcgtcg
    24601 tcatcatgac atcaatctgt ttatcttgca tcacagggtt caatcccttg ctaaaatcac
    24661 caacagacaa atcagcgaca tatggaagaa gaaacagaat ccgtcgtgtt tttgtaggag
    24721 aagcaagaat ctgttccgga gaagcggatt taacgaaact gcctttcccg cgaacgcgat
    24781 aaataagtcc taattgctct aattcagtca atgcacgttt ggaagtgatt ctgcttactt
    24841 tataggtacg tgaaagttct ttttcagttg gtagttggct gtctacaggt aattcaccag
    24901 ttaaaatagc cgtttgcaaa tcattaaata ttgtctggta caatggttga gtcatataaa
    24961 cacctcttaa tgatatatca tattcaaata ataccataat ggatgaaaaa gtaaataaaa
    25021 agatattttt tgaaagcgta gacataattg gctgtatgaa gtataatctt taaagatata
    25081 tcaaatagaa agaaggtttt attagtggtt tataaagata taccaaaatc ggtgcagaaa
    25141 tttatggaag atatcacaca aaaatgtggg gatatccatg cggactgggc aaagaatttt
    25201 acagcagcat ttgccaatac tctgttgaca acagtcaaaa agcaaaatga cggaaccacc
    25261 tttttattga cgggagacat tccagcgatg tggttaagag actctactgc acaagttcgt
    25321 ccttatttag tgattgcaaa agaagatgag gaattagctg acatgatcag cgggttagtc
    25381 aaaaagcaat ttttttatat caatatcgat ccatatgcga atgccttcaa cgaagaagct
    25441 aacggtgctg gccaccaaac agatcataca aaaatgaacg attggatctg ggaaagaaaa
    25501 tatgaaattg attctttatg ctacccaata cagttggcct atcttcttta caaaaataca
    25561 ggaaaaactg atcaattcaa tcaatctttt attgaaggta tcaaaaaaat cttggacgtg
    25621 ttcaaaacag agcaagatca tgaacactct ccttatacat ttgtacgaga cacgacaaga
    25681 aaagaagata cgctgatcaa tgacgggcga ggtgcaaaaa cagcttttac aggaatgaca
    25741 tggtctggtt tccgtcctag cgatgatgct tgccgttatg gatatctggt accatccaat
    25801 atgtttgcgg tggtcgtgtt aggttatgtg gaagaaattt ttcaaacgat actcgatgat
    25861 aaagaaattg tacagcaaac acagaaattg aagaaagaga tccaagaggg gattcagaat
    25921 cacggacgaa cggttaatca aaaagggcaa acgatttatg catatgaagt agatggaatg
    25981 ggaaacgcca cagtcatgga tgacagtaat gtgcccaatc tgatcgccgc accttacttg
    26041 ggatattgtt ctgaacaaga ggaagattat ctgcaaacaa gaagaacatt gttgagtaaa
    26101 gaaaatccat atttctatga aggaaagtac gcaaaaggaa ttggatcctc ccatacgcca
    26161 gaaaaatacg tttggccgat tgcattagct atggaaggaa tgacaacgtc agacaaatct
    26221 gaaaaagagc ggatattgaa tttacttgta gcaactgacg caggaacaca ccttatgcac
    26281 gaaggattcg atgtgaacaa tcctgaaaat tatacgagag aatggttttc ttgggcaaat
    26341 atgatgtttt gcgaattagt catggattac tttgatatta gagtagaaaa gtaggaggta
    26401 tcgtaaatga aaaagaaagt atatatcatt tctcatagcc actgggatcg tgaatggtac
    26461 atggcttacg aacagcatca catgcgactg attgaattga tagacgatct actagagcta
    26521 tttgaagttg atccgtcctt caacagtttt catttggatg ggcaaacgat tattttagat
    26581 gactatcttc aagtaaggcc agaaaaaaga caggcaatcc aacaagcaat caatgaagga
    26641 aaattgagaa ttggaccatt ttatatcttg caagatgatt ttcttatcag ctcggaatcg
    26701 aacgtgagaa atatgctgat tggcatggaa gaaagccgta aatggggaac ccctgtcatg
    26761 ttaggctatt ttcccgatac atttggaaat atgggacaga cgcctcaatt aatgaaacaa
    26821 gctggtattt cagcagcagc gtttggccgt ggtgtaaaac caattggctt tgataatcaa
    26881 gttttggaag cagaaaacta ttcttcccaa tactcagaga tgtggtggaa aggccctgat
    26941 caaacagcaa tctttggtct gctatttgcc aattggtata gcaacgggaa tgagattcca
    27001 gtagaaaaag aagcagctct tgccttttgg aagcaaaaat tagcggatgc agaacaatat
    27061 gcctctacga accacttatt gatgatgaat ggtgttgatc atcaaccggt acaaaaagat
    27121 atatcaaaag caatccatct cgcaaatgaa ctgtttccag attacgagtt tattcattct
    27181 aattttactg actatctgga agcagttcaa aaagatgtgc cagaagactt aggctcagta
    27241 gaaggagaat tgacaagtca agaaacggat ggctggtata ctttagccaa tacagcatct
    27301 gctcgggttt acttaaaaca atggaatacc aaggtccagc gacagctaga aaatattaca
    27361 gaaccacttg cgacgatggc ttatgaagtt agtggaaact atccccatga tcaattagat
    27421 tatgcttgga agacattaat gcaaaatcat cctcacgaca gtatttgcgg ttgttcagtc
    27481 gattctgtcc atcgggaaat gatacctcgt tttgaaaaag cagatgaagt tggaaagtat
    27541 cttgcacaag attccttaga acagctgacc gcagcaatcg atacgaccgg attcccaaaa
    27601 gacagctttc catttgtcat tgtaaatact gcaggaatgg ataaaactgg tgaagcagaa
    27661 ataaccatcg aattagaaag aaaaagattc gctgaaggaa tacctgaaca actatatcaa
    27721 gagttagaaa atttgcctaa acgtaagtat catgtagaaa caaaaagtgg ggccactata
    27781 ccagctattt tatctgaaga aactgtccaa tttggttatg atttgccgaa agaccgtttc
    27841 cgtgtgccat atatggcaag aatgatcaaa gtgactttac cattagaaaa catgccagca
    27901 ttttcttggg aaacattcgc gcttgtagaa ggggaagcga aagcagaaga aaaggaaaca
    27961 atgattcatc agagtggacg aatcatcgaa aacggccctc tacatttgac gattgaaaaa
    28021 aatggtacca ttacaatgga agatcgcaaa aacaaacgaa agttgaataa tctgcatatt
    28081 tttgaagata tcggcgatat aggaaatgag tatatcttta aacaaccttt ctgtgatcag
    28141 ccaatcctat ccagtaacaa agaaaactct gaagtaaaag tattagtaga tacaccggaa
    28201 atagcaaaaa taagtattct acaggaaatg gaaatccctg tttctgcaga cgagcgttta
    28261 gaaaaagaac agcagatggt tgtggaattt cgttatcgaa aagcagaacg atccaaagaa
    28321 aaacgaatcc ttcaaataaa aacaatcatg acaatcagaa aagatagtaa aaaaatcgac
    28381 tttgaaacga ctctcgacaa tcagatgaaa gatcatcgct tacgtgtctt gtttcctaca
    28441 aagttacatg tggaacatca cgaagcagat agtatttttg aagtggtgaa gcgcccaaac
    28501 catgtgtcta aaagttggga aaaccccacg aatccgcaac atcagcaagc atttgtcaat
    28561 attcatgatg aagagtacgg tgtaacagtc ggaaactttg gtttgaatga atatgaagta
    28621 acagaagacg gacaaatcgc agtgacgcta cttcgcagtg tgggagaact aggcgattgg
    28681 ggatattttc caacaccaga agcacagtgt ctaggtgagc atcggttcaa ttacagcatt
    28741 gaactacatg gaccagaaga aaaattctct acttatttgc atgcatacgc agcacagatt
    28801 cctttttcca ctcaacaaat caaacatcat gaaggaacat tgatttcaaa acaacaatat
    28861 ttaacgataa aaagtgaaac gtttgcaatt acagctttga aacgcagcaa gtttagtgat
    28921 aaagtagtag taagaggttt caatatgtct agtcatttag aaaaattgga aataactaaa
    28981 gacaatggaa aaactgtcat attgaatctg cttgaagaac caacgaagca agcggttgtc
    29041 ccaataatcc aaccttatga gattcggaca atcggattcg aggaggaaaa cgaatgtatg
    29101 gcggaataga agcaggcgga acaaaatttg tgtgtgcggt ttcagatggt ggagaaattt
    29161 tagaaaaatt aagtatccct accacaacac cagaagaaac attggatcag gtatttgaat
    29221 tttttgatcg ttatgaattg aagggaatcg ggattggctc attcggcccg attggtatcg
    29281 acaaaacaaa agataattac ggatacgttt tggcaactcc caaaaaaggg tgggcacaat
    29341 tcgattttct tggcacaatc aaggaacgct acaatattcc tgctgcttgg acaacggatg
    29401 tcaatgctgc tgcatatggg gaattgaagc aaggagcagc acaaggaaaa aatagttgta
    29461 tttaccttac cgtgggaaca ggaatcggtg caggtgtcgt gatcaatgaa gaaatttttt
    29521 cagggatcgc acatcctgaa atgggacata tttgggtgaa acgccatcca gatgaccatt
    29581 atgaaggtac ttgtccttat cataaagatt gtttagaagg attagcagca ggaccgtcta
    29641 ttgaggcacg cacagggatc aaagggcaag atctgccgca agatcatcct gtttgggcta
    29701 ttcaggctta ttatattgct caagcactga tcaactatac gttgacgtta gcacctgaaa
    29761 agattatttt aggcggtggg gtaatgaatc aggatcatct ccttcaaaaa atccgtcagc
    29821 aatttgttga attgatgggt ggttatatgg aaacacctca agtaagtgaa tatatcgttc
    29881 gttggggact gccgaatgaa agtgggatta tcggtagttt gctgttagca gaacacgaac
    29941 atcaaaagta agcagacaaa ttacacaaaa agaacagagt cttatcatta tctgctgata
    30001 agactctgtt cttttttatt tctattccca taatccaggt aaagctttac cgactatttt
    30061 ttcttgagta aatggatgga tcatgtgcag ttctaatgca tggagcatca gtcgttcagc
    30121 tgttctattt tggtagagag ggtctccaac gatcggatgt cccttatgag ctaaatgaac
    30181 tctgatctgg tgtgtacgtc cggtttccaa aacacaatag acttgtgttt gttcagaaag
    30241 tactttgttg acagtgacat gtgtcaaggc gctctgacca tttttatcat ctacgaccct
    30301 ttttctccga tcatggcgat ctcgtccaat tttattggtg atggtcatat ctttggcaat
    30361 ttctccccat acgatggctt gatatctccg atagatcatt ttttgttcca acattcttcc
    30421 aagtatcggc aagacaaatg gattttttgc aaataaaacg gcaccgcttg tttctttgtc
    30481 caagcggtgg acaacgtaag gtcgttgctg tttagtgtcg agataagcag ctaaatgatt
    30541 gagtaaggta ctgttttctg tcggttcatt tggatgagtc ttcattcctg caggcttatc
    30601 cactataatc aaatgctcat cttcatataa aacacggatg tgcttcgcat ctcctaattg
    30661 aacttcttga taattataat ctgtttcttc gaataacaat gtgatctggt cattgccctt
    30721 tacttcatga tggaacaaag caggttgttg attgatccaa acgtttttac gtatacgcaa
    30781 aaagtgtcga acctttctag gaactaacca ttccttttca agcaagtcgc gaattgttgt
    30841 ttcaggatgg ttcataggta aagtgatcgt gattttcaca agtgtactcc tttcagtgca
    30901 attgcagact aattgtacca tttttaaaac atatttaaga aaaaacttac atttttctgt
    30961 tcattcttat gttactctac ttgcactaac gtaaaaaaac atattgaact acaacttaag
    31021 caggaagaaa acactctttc actatggtaa aataagacga ttgaatcaaa tagacaggag
    31081 aagaagcaaa tggattttca aagcatttta gaaaagataa aagctgcttt gatcagtttt
    31141 tggcgttgga tcagaccata tctcggacaa ttccatcgat ggcgaaagcg catctggaaa
    31201 aaatatcatg tgaacaaatt gattttgctg attggtttga tctgtgtctt agtgacaagt
    31261 atctacctct ttattttagc aaaacaagca aatgtcgaaa cgttaaaatc aggattaagc
    31321 caatcgacag tcatttatga taagaaaaac gaagaagctg gcacactgta cgcccaaaaa
    31381 ggaacatatg tagaattaaa cgcgatttct ccctatatcc aagatgcagt aatctcgaca
    31441 gaagatagga atttttacga gcatcacggg tttgatatca aagggattgc ccgtgcagca
    31501 gtccgaatgg tcttgtctgg cggaacatca gggggcggag gaagtacgat cactcagcag
    31561 cttgccaaaa atgcgtatct gacattagac caaacgtttg atcgaaaagc gaaggaatta
    31621 tttttggcaa tcgagatcga aaagaagtac agcaaagaag aaatcttgac catgtatctt
    31681 aacaatgcct attttggcaa cggggtatgg ggtgtccaag acgcttccag aaagtatttt
    31741 ggagtagatg caacgaatgt tacgctgtct gaggctgcta ctttggcagg aatgctgaaa
    31801 ggtcctggta tttataatcc gatcgatcac ccaaaaaatg cgaatgatcg gaaaaacaca
    31861 gtactaagtg taatggaaga aaacggaaaa atcacaaaag aacaagaaca agcagaaagc
    31921 cagacagata tcagcgcgta tcttaatgac acatacgata attcagattc tggttatcgt
    31981 tatccttatt attttgatgc agtgatcgat gaagcagtta acgagtatgg tcttgatgaa
    32041 gaagatgtca tgaataaagg ctataaaatc tatacagctt tagatcagga ttatcaaaag
    32101 caattagaag ctacttatca aaacagtgca ttattcccag caaatgcttc agatggtgcc
    32161 atggtccaat ctgcttcagt agctttagat ccgaatacag gtggtgtaca agcactagta
    32221 ggacgaagag gagatcatgt ctttagagga ttcagctttg ctacacagat gcgtcgttca
    32281 ccaggatcaa cgatcaaacc attaagcgta tatacaccag cactggaagc tggatacaag
    32341 ccttccagta tcttgaaaga cgaaccgcaa agttattacg atgcgcataa ttttgacggg
    32401 acataccaag gagaagtacc gatgtatcaa gctgttgcac aaagtttgaa tttgcctgct
    32461 gtatggcttt tgcatgagat cggtttacaa aaaggatatg ataaagcaga agaattcggc
    32521 ctgcctttag caaaatctga taagtattat ggcttagccc ttggtggact ggaaaaagga
    32581 gtatctccat tgatcatggc gggtgcttac agtgcatttg ccaatgatgg tcagatgtat
    32641 acgccgcatc ttatcacgaa aatcgttgat tcgactggag cggtgatcgt cgataacaca
    32701 aacaaaaaac caaaacaggt catatcaaag gaaacagccg atgaaatgac cagcatgctt
    32761 cttggtacat tttcaaatgg aacagcggct gctgcgaatc ctgcaggtta tacgatagca
    32821 gggaaaacag gaaccacaga aactaatttc gatgccacaa aagcaaatga ccaatggatg
    32881 atcggttaca cgccagacgt agtgatttcg acatggatgg gttacgaaga atccagcaag
    32941 ctgcactatt tggaagggac aagtggaaat gttgtcggga aagtcttcaa atcagcggca
    33001 gaaggcatcc ttccgtatac ttcaaaaaca ggatttagtg tagcagatgc ctatgccaca
    33061 ggaggacaag tggtagctgc agatcaagtt ggaaacacag gaaattctaa tggagagtct
    33121 ggaaattggc aagataactt gaatcaatat ggtgaacaag caaaagaagg gctgaaaaac
    33181 ttcggcgata tggtgaaaga aggcgttcag ggaattggtg aagcggctaa agacctctgg
    33241 cggaaatatc aaggagagta gcccctttga aaacgaataa ccccatgtta caatagagag
    33301 taactactaa aagaaaagag ggcataacat gtcaaacatc tacgatagcg cgaatcaaat
    33361 cgaacgtgag atccgtgaat taccagaatt caaagcgttg aaagaagcgt acgagaaagt
    33421 acaagcaaac gaagaagcac acaaactgtt caaagacttc caagcgatgc aaatcgaatt
    33481 acaagaaaaa caaatgagtg gacaagagtt cagcgacgaa gatgcaatga aagcacaaga
    33541 aatggctgtg aaaatccaat cagaagaagt aatcaatgac ttgatgcaaa aagagcaagg
    33601 cttcagtaca atcatcaatg acttgaaccg tatcatcatg acaccagttc gcgaccttta
    33661 cagcgaataa aactatttta tgaaacaaca gagggtgtga aagagacgtt cggccttgag
    33721 cgatgacaaa attgcttctt attgccgata ggctgtctct tgaacaccat ttaccaagag
    33781 gtcgtgagcc taccgtttaa ctctgagtta caaggaacaa tttcaaaaaa cagcttctca
    33841 tgtttgatga ctattgtgac ttgtgaccga ggagttggca ggagaacacc gtttattcag
    33901 aaataggagc tacgacaaaa gtcttgtcat agctcctatt ttttgtcatt catttttcaa
    33961 gcggtagaaa tagccttttt gagctaacag tttttcgtaa gtacctattt caacgatttt
    34021 tccttcatcg atcacaacga ttttatctgc ctctttgatc gttgaaagcc gatgagccac
    34081 aataaaagta gtgggtgttt ctgtcaggta attcagggct tgctggattt ttttctctga
    34141 ctggttatct aaagctgagg ttgcttcatc tagcaaaatg atttttggct gacgtatcaa
    34201 cgctcgtgca atggagatac attgcttttg gccaccagac agcttgttgc catgttcgcc
    34261 tactaaggta tccagaccat caggaagctg gtcaatcagt gaagaaagtt gcgcagcttc
    34321 gatgacttca tctaaacgtt cttttgatac gtttttcaag ccataagtga tgttctcttt
    34381 gatcgaagcg gagaacaaca aagtggtttg aggaacaacg gctaaataat ttcgcacact
    34441 tcgcatatcc agttcatcca aaggctgatc atctagaaaa atttttcctg aagtcggctg
    34501 gataaatcca atcaataagt taaggagtgt agattttcca gagccagatg gcccaacaaa
    34561 agcaattgtt tctccagctt tcacctctag agaaaaatga tcgataatcg gtttgagtga
    34621 gtccggataa agaaaagaaa catcctcaaa acgaaaattt ccatccaaat gttggatttt
    34681 tttcttaccg tgataagttt ctacatcgcc agcaatcaac agttcgccaa tagaagtgat
    34741 agattccatg ccttttgaaa tcgtaggaat cagcgtgatc attccagaaa cggaattgac
    34801 gatcgtcgtg aaatacgtct gatacatcac gacatcgccg ggttggatgc gcccttgtaa
    34861 agctaatatc ccagtgaaaa tcaaacaaat cagctgaaat atctgaaaac tagcccagct
    34921 gactgatccg aaaacagatt gcaggatatc taagtggaat cctttttcat agatttgagt
    34981 aaaatgctta tttaagcgag ttgtttcgtg tttttctaac ccatgagctc gagcaatcgg
    35041 tgctaattcg atcacttcga tcacttttgc agaagtctct tccatttctt gcctgaagtc
    35101 tgagttggac tgtctgatcc ttttacggaa aaagagagta acgaatgcgg cgagaggaac
    35161 agtcaataag aaaaacatga aaacaacagg acttttataa agcgtgatac ctaaagctac
    35221 gaataaattg acagcgatat ttaatccgct gacaaatact tgagaggaca acgtttggat
    35281 agcttctaca tcacggatga tctttgactg gattcgtcca gattgcaatt ctttttgata
    35341 aggaatagat agttcttgga ttttcttgat aagggtcgct cttagtccag cttctacttg
    35401 acggatcgaa gtgctatggt attttacatg aaaataattc gtcaatatat tttgaccaat
    35461 cagacaagcc atcaaaaaag cgttgaaata aatggcctgc aactgagaag aatctttcga
    35521 tgaagctgca ttgattacat tggcaatagc gatcggcatt aaccaagatg gaagatgttt
    35581 gataataaaa aagagtcctg acatggcaaa acgccaataa tttccctgat aaagatgcag
    35641 caatgtctga aaagcatttg catgatattt ttgataactt ttctgtattc gttcctccat
    35701 aatcttctcc ttaacaatct tggtaaataa actgcaagtg ttctattgct tcaggaacat
    35761 aaatctcagg agtagcttca agagtggcgt acattagagg acgctcatat ttgatataat
    35821 gcagtaccgg gcggaaatct aaacagccct tgccaacagg taccatgcgg atctttccat
    35881 tttccatgat atagtctttg agatgaagcg ctgtaatata accatgtaaa ttgtccaagg
    35941 cacctcggat cactttttcc tgatcagcat gattttcttt atgcatcaaa ttcgcacaat
    36001 ctaaaatgat ttttaaattt ggtgaagcta cttcatcgat taaacgttta gctaactgat
    36061 agttatagat tggatgattg atccctgctt caacagcaac agttacgcca aaattttctg
    36121 catgagcgac taattcaatg atagaatttc tggcgatctg ataagcttct tcagtaaaat
    36181 tttcttttgt gtaaccgaca gagacactac cggtttccgt agcaatcatc ttggcttgat
    36241 aggcagagca cagagcaatt tgctgtttga aagttgccaa tgcttgtgca cgaacttctt
    36301 gatttttgct ggataggttg atatagcatc ctaaaatgga aagttcgata ttgttttttt
    36361 ggaagtattc accaaaatag gcagcactcc caagtgtcat gtcagttaat ttaagttgtt
    36421 caggaaatgc tttttgtggt gcaaattgga tatgggacaa gtgaagctgt gatacttttt
    36481 gaacgatttc ttcacgattt cgaacatcta aatcgtgggc acggatgcct aaatttaatg
    36541 gaaacatctc aaactccttt ttgtaaataa ttagcaagag gcagacggga ttcttttatc
    36601 aattcaacaa ttattcgggc aactttatca gcgccttctg gactaaagtg ggtattgtct
    36661 tttagtcctt ctgggtaatt tttacaagta tcaggcataa gatgcaggta gaattttttt
    36721 gtctcttctt ttgagaaggt ctggaacaat tcttgtgatc ttgagaacac atctaaaaga
    36781 ggaatatggt gtttctcagc aaatgcacgc atcgcttttg gatagtctcc aagtatatct
    36841 ggattcaatg ttccgttttc taagtaatct cgtcgcgtca cagatgtcag taagaccatt
    36901 tggacgttcg ctttttttgc gcattcaaaa aatatctgca gattttttaa atagctaccg
    36961 aagggaagcg tacctctttc ttcttggatt ttctggtcgt tatgtccaaa ttgtacaaat
    37021 aagaaatcat tcggctgaaa ctgtgaagct agacgggcta accttccttc ctggataaat
    37081 gatttggtgc tgcgtccatt ctttgcttgg ttattgatct ggatctcggg tgaaaaataa
    37141 gcagagattt tttctcccca acctgtctcg ggacgcttgt tttcatcttt ttctgccgca
    37201 gtagaatcac cggcaatatg gatagtaggc atgttatctc ttctttcata aaggtttata
    37261 ggcaatgaag cagaacagca agagaagaag acggagcaa
//
LOCUS       DAYYFU010000022        34869 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000022 DAYYFU010000000
VERSION     DAYYFU010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.022"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..187)
                     /locus_tag="ACHA34_001320"
     CDS             complement(59..187)
                     /locus_tag="ACHA34_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8791972.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACHA34_001321"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACHA34_001321"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8791973.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="ACHA34_001322"
     CDS             complement(1674..2729)
                     /locus_tag="ACHA34_001322"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8791974.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACHA34_001323"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACHA34_001323"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFM8791975.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACHA34_001324"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACHA34_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFM8791976.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACHA34_001325"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACHA34_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFM8791977.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACHA34_001326"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACHA34_001326"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFM8791978.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACHA34_001327"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACHA34_001327"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFM8791979.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="ACHA34_001328"
     CDS             10491..12050
                     /locus_tag="ACHA34_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFM8791980.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="ACHA34_001329"
     CDS             complement(12161..14092)
                     /locus_tag="ACHA34_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM8791981.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACHA34_001330"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACHA34_001330"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM8791982.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="ACHA34_001331"
     CDS             complement(15021..15773)
                     /locus_tag="ACHA34_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8791983.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="ACHA34_001332"
     CDS             complement(15932..16141)
                     /locus_tag="ACHA34_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791984.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="ACHA34_001333"
     CDS             complement(16152..17480)
                     /locus_tag="ACHA34_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791985.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="ACHA34_001334"
     CDS             17616..17771
                     /locus_tag="ACHA34_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFM8791986.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACHA34_001335"
     CDS             complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACHA34_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFM8791987.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACHA34_001336"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACHA34_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFM8791988.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACHA34_001337"
     CDS             complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACHA34_001337"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFM8791989.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="ACHA34_001338"
     CDS             complement(21455..22387)
                     /locus_tag="ACHA34_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791990.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="ACHA34_001339"
     CDS             complement(22544..23203)
                     /locus_tag="ACHA34_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM8791991.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACHA34_001340"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACHA34_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM8791992.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="ACHA34_001341"
     CDS             complement(24244..24534)
                     /locus_tag="ACHA34_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8791993.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24943..26748
                     /gene="glmS"
                     /locus_tag="ACHA34_001342"
     CDS             24943..26748
                     /gene="glmS"
                     /locus_tag="ACHA34_001342"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFM8791994.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26820..28175)
                     /gene="glmM"
                     /locus_tag="ACHA34_001343"
     CDS             complement(26820..28175)
                     /gene="glmM"
                     /locus_tag="ACHA34_001343"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFM8791995.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28199..29368)
                     /locus_tag="ACHA34_001344"
     CDS             complement(28199..29368)
                     /locus_tag="ACHA34_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFM8791996.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29365..30252)
                     /gene="cdaA"
                     /locus_tag="ACHA34_001345"
     CDS             complement(29365..30252)
                     /gene="cdaA"
                     /locus_tag="ACHA34_001345"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFM8791997.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30441..34133)
                     /gene="nifJ"
                     /locus_tag="ACHA34_001346"
     CDS             complement(30441..34133)
                     /gene="nifJ"
                     /locus_tag="ACHA34_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFM8791998.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            34332..34853
                     /locus_tag="ACHA34_001347"
     CDS             34332..34853
                     /locus_tag="ACHA34_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFM8791999.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         9400 a         7569 c         6284 g        11616 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcctgc ttttttgaag taagcgtcat aataacccgc gcttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tcagagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 aattggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcctcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgtttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttccgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgatagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcgattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg cgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ctccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggaa attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat gtcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt taccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccggtcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatacgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaatgtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtgggg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagaggc tgggaaaacc cagcctcttc ttttttattt ctatgcttct gctactgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgccg tgaggagcca
    12301 gtaatttgat tccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcaactc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcaaagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccagcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagtaat tttttcccat acggaatgat ttcttctaca gattggaagg ttgtgttgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaac ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagctggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cctaagccat tgatctcagt ttcagtgtct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg gatccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattcaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctgcgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctacta gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcag aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgtga tcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt ggatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttcaaatc tttttataat ttaattataa
    17521 ccaatgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc gccctgaggt ggcacacttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcaagttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttaaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttctttt aacacggaac
    21661 taagcagcgc atcttcattc ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgcc actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcata ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaacgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccca
    22621 gatgttgctt tttctgatta aagactgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttgg ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagagac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaggc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgtata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttggagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt cttttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgttaatt catcttctac
    24361 cgtaaactca cgagcttgag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaaggacat ttgacaaaga atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaaagga ggatagttat accttttagg atagcgccag gtctgtgtaa
    24781 acagatgacg aggagagggt ttatcgaaag attcggcgga tggccctagg gactgcactc
    24841 tacagaaaat ccacaaaaaa tgtctgcaaa gataaaacaa aaggattttc ctgcagctgg
    24901 aagcattcat acgaattcat tatagaaagt aggatgtttt ttatgtgcgg aattgttgga
    24961 atgattggat taaaaaatgt aacaccagga ttgattgacg gtctagaaaa attagaatac
    25021 cgtggttatg attcagctgg tatctttgtc tcagatggca caactgatta tctggtcaaa
    25081 gcacaaggca gaattcaaaa tttgaaaaac aaaataaata ttgatactac aggaaatata
    25141 ggtattggtc atacacgatg ggcaactcac gggcaacctt ctgaagaaaa cgcccatcca
    25201 catacttccc aaagtggacg cttcgtgctc gttcacaacg gcgtaatcga aaactttgaa
    25261 gaacttaaaa ttgcttattt agcaaatgac cactttattg gtgagaccga tactgagatt
    25321 atcgctcatc taattgaaac tttcgcgaag gatactacaa cgcaagaggc gtttttaaaa
    25381 gctttgcggg tcatcaaagg ttcttacgct ttcgctttga ttgatcggac agcacctgat
    25441 gttatttacg tcgctaaaaa taaaagtcca ctattaatcg gactcggcga tggattcaat
    25501 gtgattgcca gcgatgcaat ggctatgctt gctcatacga aggaattcgt tgagattgaa
    25561 gatgaagaga tggttaccgt cacatcagaa aaagttatta ttcaaaattt tgcaggtgac
    25621 atcgtagaac gttcttcatt cgaagcacaa gtagatgctt cagatatcga gaaaggtacc
    25681 tatccttttt atatgctgaa agaaatcgac gaacagccta ttatcatgcg tcgtatcgct
    25741 caaaaatacg taacagaaga caatcgtgtc tctcttgatc aaaagctggt cgatacattg
    25801 tctgatagcg atcgaatcta tatcgttgct tgcggaacta gttaccatgc ggggttagca
    25861 ggtaaacaga ctttggaaaa attgacacag attcctgttg aggttcattt agccagtgaa
    25921 tttggctata acacacctct tctttcaaaa aaaccattct ttattttctt gagtcaaagt
    25981 ggcgaaacag cagacagccg ccaagtgctg gtaaaaatca accgtctcgg ctacccttca
    26041 cttacgatta caaatgttgc tggttctact ctttcgcggg aagcatcctt tacactgcta
    26101 ttgcatgctg gacccgaaat cgcagtagca tcaacgaaag cttatactgc acaaatagct
    26161 gtactaactt tattagccaa agctatcggt gataaaaaag aatttgaaac ttctcttgta
    26221 ttcgatatca ctcatgaatt gagtttagtt gctaatgcga tggaatcggt catcgcccaa
    26281 aaagattatt tagaagagtt ggcagaagta tatctcagcg atacaagaaa tgccttttac
    26341 attggacgtg gtgctgacta caacgtttct cttgaagctg ctttgaaatt gaaagaaatc
    26401 agttatatcc aggcagaagg ttttgccgct ggcgaattga aacatggaac gattgctttg
    26461 atcgaagaag gaacaccagt gatcggaatc atcagtgaag aagtgactgg tgcgcatact
    26521 cgaggaaacc tgaaagaagt cgaaagccgt ggcgctaaaa cactagttat tgtctcagag
    26581 ggattagaaa aggaaagtga ccagctggtg ctaccaactg tccaccctta tctaactact
    26641 ctaaccacag tcgtaccgac tcaattactt gcatattacg caacgctatt acggggattc
    26701 gacgttgaca aaccacgtaa tttagcgaaa tcagtgacag tagaataata aaagaacaag
    26761 gaccagtgtc agacatctga cactggtcct tgttctttta ttttttatga aaatatctct
    26821 taatccagtc cgatttcttc tttgactact gccgcgattt tatcgacata gtaattaact
    26881 ttctcatcag taggcgcttc tgccatcact cgaagtaatg gttctgtacc ggaaggacga
    26941 actaggattc ttccttctga tcccatttca gcttctgcct cttcaatcac ttttttgatt
    27001 gccggaacat ccatcgcacc gtttttatca cttacgcgga tattcactaa tttttgcgga
    27061 tagattgtta cctctgctgc aagctctgat aatttcttac ctgtctgttt catgatattc
    27121 agtaactgaa ttcctgaaag catcccatct ccagttgtgt taaaatctaa gaaaatcatg
    27181 tgcccagact gctcgccgcc aaagttataa tcgtttttgc gcatttcttc tacaacgtaa
    27241 cgatcaccaa cttgtgtaac gacatctttc aaaccgattc cttccaccgc tttgtggaat
    27301 cctaagttac tcatgacagt cgtaacgatc gtatcttttt tcaatcgatt tttttctgcc
    27361 aagtatttag cacagataaa catgatttta tcaccgtcga caatgtttcc aagttcatcc
    27421 accgcaataa tacgatcgcc atcgccatcg aaagctagtc cagcatctgc tcctttttca
    27481 acgaccatct cagctaaacg ttctggatgg gtagagccta cgccgtcatt gatatttaac
    27541 ccgtctggat ttgtgcccat tgtataaaaa tctgtttcaa gatcggcaaa caaacgatta
    27601 acacttgttg aagttgctcc gtttgctgca tcgacacaaa tggtcaaacc ggaaaggtca
    27661 ccgttgatcg tttgtacaag gaattgagaa tatttcaata agccttctgg aaattcttct
    27721 acagttccca atccttcagc tgatggacga ggcaattcat ccacttcagc atctaataat
    27781 gcttcaattt ctagctcttg gtcatctact agtttaaaac catctgcacc gaaaaatttg
    27841 attccattat cttgtgctgg attatgagaa gcagaaatca tgacccctgc gcttgctttt
    27901 tgcaaacgtg tcagataagc aacacctggt gtagagatca cacctagctg gaacacttcg
    27961 atacccaccg ataaaagtcc tgaaatcagc gcttcttcta acatttgacc tgagattctt
    28021 gtatcccgcc ctactaacac gcgtgggcgt tttccttctt cttcatgctg gcttaggaca
    28081 taacctccgc accgtcccag cttaaatgca agttctggcg taagttcctt gttggcttca
    28141 ccgcgtacgc cgtctgttcc aaaatattta cccatagttg taaaattact cctttaactt
    28201 aattttctga ggaatttgtt ctgctcattt gtgttgattc gcttgtttct gatgtttctt
    28261 ctgtacttgt ggtcgagctg cttgtcccag attccgaact ggaagaaatg gttgttgtcg
    28321 atggttccgt tcctgaatca gcagatgaaa tacttctact cgtacttgta ttttcttgtg
    28381 tccgactgct cgtcgaagca gagctactgc ttgacgtttg caacacgggt gtaatctcca
    28441 cactaactga ttcgggcttg atacttaacc cctcttctac tggtatttca attttacgag
    28501 ttgttcgatg attgatacct gagacattga tcggtacagc aatactgtca ccgatcgatg
    28561 caagcaaatc gctagagccg cttacttctg cttccagttt tgaaaaactg aaatcatagc
    28621 tttctacccc ggcagctggt gttccttgct gtatcccata aagtcgtacc gacttgcttg
    28681 gcttgatcga atcagctctc actgttactt gcactggatc agagacgata cttaatactt
    28741 cccctgctgc attcagcgcc tgtattggga cctcactatt aatcccatca tctgtgacca
    28801 tcgaagcgtc cacatcagct actactcgtt tgatttctgc caatgttttg tcacctgtag
    28861 tgatagagac ttttttagga ctaacggaag tatcatcaag ttcatatcca gatggtgtga
    28921 acgttgaagg aatcaacgtt tccacatcga aatcttttat tacctttttc tcaaccgtta
    28981 ctgtaatagt cgatggttcg atctttgctg tgacagcact gctcaaattt tgaattttta
    29041 gcggaacttc atgtgttcct tcgcccagct tagtgatatc tgctgtgacg cggaacatcc
    29101 gtgtatcttc gtttgcctct gcattcaact gaactcggtt tgcactggaa agtttgacag
    29161 tcactgcatt ttcatatcca tggatatagt agcgctttga atcataagaa atcttgatcg
    29221 gcacccccgt aactgtttga ttatacgctt catttccaga aagcgtattt ggaacggact
    29281 ggccatttgc attgaagtac agcgcaatac tgaacagcaa tgcaataaaa ctgtaaagaa
    29341 tgcttgtccg tttctctttt gagatcattt gcgatcccct cccttagtca taccgtctag
    29401 gaaagattga atcacgtttt tcttgttttt tgttttctct tcagcaatta gttctctacg
    29461 caagattgcc aaatattctt gctgggataa gccagccatc agttcattat ctaacgtgat
    29521 gctgacgtcc ccggtttctt cagagacaac gatggtaatc gcgtcactga cttcgcttat
    29581 accgacagct gcccggtgtc gagtcccgaa ttctttaggg atcaacatac tttctgatag
    29641 aggaagatag gcgcttgcta ctgcgatctt tccttctttt acaataactg caccgtcatg
    29701 cagcggtgta ttaggaataa agatattgat cagcagctcc ccagtgatat ctgcatctaa
    29761 agcaatccct gtctcaatat actcatctaa tccagtatga cgttcgatag tgatcaacgc
    29821 accgattttt cgcttggaca tatattggat ggctttgtca aaagacaaga tcatcttctc
    29881 atcttcctgc tgttcgcttt ttgattgttt gaaaagcgaa ctgcgtccaa gatgttccag
    29941 tcctctccgg atttccggct gaaaaatcac gatagcagct atcaccccgt acgtaatgac
    30001 ttggttcatc agccaagaca aggtgtgcag accaatgatt tccgctacga ttcggataac
    30061 aataaaaata gctacacctt tcagaagctg aatcgctttt gtgcctcgca gcagttggat
    30121 cagtttgtat acaagatacc agacaaccag aatatcaagt atgttcacca aaaaattcag
    30181 agagaacaag tcaccggaaa acatttgctg ccaatagtcc aaattaaaaa gttccccgat
    30241 ttgaaatgac ataaaaagcc tctcttctct tttgagattt cctctctagt atatcatatg
    30301 ttttaatttt agaggctgat atgctcgtat ttttcttgag aaaaacgcat aattttcaag
    30361 aaaattgcaa gtatgcgttt tatttttctc tgcaataaat atttttaaac ttcagagaat
    30421 tattcattat attttttctg ttatttttcc tttttttctt ttgtagcttg ttccattttt
    30481 ttacgtcgtt ctgctctggc agctcttcta gcagctcttc tttcttctgc ttcaaggtcc
    30541 gtcgtttttt cttttctagt cttaacgggt acatcgatag attcctctgg ttcaagtcgt
    30601 ttcttgattt tttctaattc tcccgtcagt gcagcatagt tgtaaaaacg tgttttagcg
    30661 tcgtggacag tcttttcaaa taaagctttt gcaagatttg gatttgcgtt atctagcgag
    30721 gaaaagcgaa cttgtttcct catgaaatca ggcatagcag agaaatcagg ttttttgtag
    30781 tccaaagtca ttgggttctt gtttttttcg accattgtag ggttatatcg atataatgac
    30841 caataaccag aagaaacagc ttcttttgct tctttaagcg attctttcat tccgccaagc
    30901 aagccatgtg tgatacaagg cgtataggca ataatcagag aaggaccagg atacttttct
    30961 gcttcttcaa atgccttgat cgtttgcatt tgattggcac ctgaagcaat ttgcgccaca
    31021 taaacatttc catatgtcat cgccatcatt cctagatctt tctttgaagc atactttcca
    31081 ctagccgaaa atttagcgat tgccgaagct ggtgttgctt ttgaggtttg accgccagta
    31141 ttggaataga cttcgttatc caaaacaagg atattcacgt ctgctccgct ggcaagtaca
    31201 tgatcgatcc cgccaaaacc aatgtcgtaa gcccatccgt ctccaccaat catccattgg
    31261 ctgtttttta cgaatagatc ctttctcttg tatatatcct ccagttcagg gaaaattgat
    31321 cgttcgcttt cgatcgcagc ttgcagtttt gctgctcttt gctgcgttcc ttcactctca
    31381 aacatgtgta ctaaccagtc ttccatcaat tctcgtagtt cttctgatac ttttggcaat
    31441 acttctttga ttgaacttgc caaagcctcg cgacgagtct gtgccgctag aagcatacca
    31501 taaccaaatt cagcattatc ttccaataaa gaatttgacc atgcaggccc ttgaccacat
    31561 tcattggtcg tataaggaga agcaggagaa gcaccgcccc aaatagaaga acagcctgtc
    31621 gcattcgcga tcatcattcg atcaccgaac atttgtgtca ataattttac ataaggtgtt
    31681 tcgccacagc ctgagcaagc gcccgagaat tcaagaagcg gttgattgaa ttgtgtagac
    31741 aagacagtat tctttccttt gactggattt gctttttgtc ttaatgtcat tgcaaaagcc
    31801 caattcacag cttgttcttt ctgttcttca taaggcttca tcactaaagc tttttctttt
    31861 gcgggacaag cctctacaca cagaccacaa cctgtacagt cttccactga aacttggata
    31921 cggtatttta cgccgtctgc gcctcgcatt tctctcacga taaatccttc tggcgcttct
    31981 tccatttcct cttcatctgc taaaaatgga cggatggcag catgaggaca aacaaaggca
    32041 cattcattac acatcgtaca gcgatcacta atccactcag gtacttctaa agcgacgccc
    32101 cgcttttcca tagaagccgt tcctaaaggc atttcgccag ttgtcattcc atttgctaca
    32161 agtgtaccaa cagagagttg atttccttcc tgtgcattca ctggttcaag tatttcttgt
    32221 atataaccag tgttcctttt attgattttt tcaggcatct ccaacgttct ccaatggtct
    32281 gggatcgcga cttgatgaag cagttcggtc gtccgatcga ttgcttgtat gtttttttct
    32341 acgatagcca ttgatttatg tccatagctt ttcagggctt cttcttttag cagcggaagc
    32401 acctcatcaa aaggaatgat gtcagctaat ttaaaaaaag ccgtttccat tgccgtattg
    32461 atccggcgtc ccaaaccgac ttcttgggcc aatctcatgg cattgatcgt atagaatcta
    32521 atgttatttt cagccaagta acgttttaat tttaagggca gatgcgtttc taattgttcg
    32581 tcgctccaaa gagtattcaa taaaaaaatg ccacctggct tcaatccttt gaccaagtca
    32641 tagctatgca ggtaagctgg tgtgtgacag gcaacaaggt ccgcatgctc gacaagatac
    32701 gctgaacgta tcggcgtatc tccaaaacga agatgagaga cagtcagacc gccagatttt
    32761 ttcgaatcat aataaaaata gccttgcgca tatttatcgg tgtgatctcc aataatcttg
    32821 atagctgatt tatttgctcc aactgttccg tcagatccaa atccccaaaa cttggcctgg
    32881 aatgtttgcg gttctgtcaa atccagactt tcctttggtt ccaaagattg ataagtcaca
    32941 tcatccacga tccctatggt aaaccgtttt ttgcgtatgc ttgggtcttt tttcaattca
    33001 tcaaaaactg ctgagatttg gtcgggagtc acatctttag accccaatcc gtagcgacca
    33061 ccaataacag ccgggcgaag ttcagaatca tataacgcac tttgaacgtc taataagaga
    33121 ggttctccac ctgcccctgg ttctttactt cgatcaagaa cagcaattgt ttttactgta
    33181 tgaggcaact tttgcacgaa tgtttccact ggaaaaggac gataaagatg gatattcaaa
    33241 aaacctgttt tccgtccgtt tgcattcaag taatcaatcg tttgttcaat cgtctgtgca
    33301 actgatccca tagaaacaat gacttcttcc gcatcgtctg ctccgtaata agtcaccaag
    33361 tcataagaag ttcctcgtaa tcggttgatc tctttcatat atttttgaac gatttcaggt
    33421 aatttatcat aataggggtt gatcgtttcc cgttgttgaa aatggatgtc tggattctgg
    33481 ttcgtaccac taacagtcgg atgattggga ttcatccctc gtttacgaaa tgaagctaat
    33541 ttctcctgat tgatcagagg agctaattct tcatattcca aaacttcgat tttttgaatc
    33601 tcgtgactcg tacggaatcc atcaaagaaa ttcaaaaaag ggacttctcc ttcaatagaa
    33661 gcaagatggg caacaggtgc taaatccatc acttcttgta cactgctttc agctaacata
    33721 gcaaatcctg tttgtcttgc agccatcacg tctccgtgat ctccgaaaat attcaacgca
    33781 tttgtcgtca ctgcccgact agcaacgtgg aaaacacttg gcagtagttc cccagcaatc
    33841 ttgtacatat tcggtatcat caaaagcagc ccttgtgaag cggtatatgt agttgtcagt
    33901 gcaccagttt tcaatgatcc atggaccaca ccagctgctc ctgcttcgga ttgcatttct
    33961 actactttta ctggttgacc gaatacgttt tcttttcctg ctgctgacca ttgatctact
    34021 aattcagcca tggtcgaact gggtgttatt ggatatattg cagcaatctc ggtaaatgca
    34081 taagaaatat atgcggctgc cgtattgcca tccattgttt tcatttttcg catgattcat
    34141 catccttgtt catattctct cttattatct gttccattat aacgtaaaac atgccatttt
    34201 tttcttttat ttataaaaat attttacatg tatctatttg aattggtcat ctaattttta
    34261 gacaaacagt ctaaatcgtt gccttttttt gtcagacatg tataaactat aagcgaaaga
    34321 ggtgttttaa catggatata tcagtgattg atgcaacgaa aacaaataca caaaaaggaa
    34381 tcctttatgg ttcatcgaat gctcctaaaa aaatggtcga attcatcaac ttagcttgtc
    34441 cgtattgccg acaatggttt gaagaatctt atgagctatt agaagaagca gtccaaagcg
    34501 ggcaattgca gcgagtgatc aaattgttcg ataaagaaaa agaaagctta cagcgaggga
    34561 acgtgatgca ccggtatctc acaatcagcg atggccaaaa agcgatcaaa gagatcaaac
    34621 aaatattcga tactcaagac gagtggaaac atctttctct ccaagaagta gcggattttg
    34681 cagtagataa actaaagctg acagaacaaa aagacgaaca gctgagccaa gctgtcatca
    34741 acgaagctga gcaagcacat attcgatttg ttccaactgt catcttgggt aaagaaatct
    34801 ttgatgaatc tatttcaata gaggaattaa aagaattgat ccaaacaaaa taaaaaaata
    34861 aggagcgtg
//
LOCUS       DAYYFU010000023        34636 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000023 DAYYFU010000000
VERSION     DAYYFU010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.023"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            591..1280
                     /locus_tag="ACHA34_001348"
     CDS             591..1280
                     /locus_tag="ACHA34_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8792000.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ACHA34_001349"
     CDS             1267..2475
                     /locus_tag="ACHA34_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8792001.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ACHA34_001350"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ACHA34_001350"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFM8792002.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACHA34_001351"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACHA34_001351"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFM8792003.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ACHA34_001352"
     CDS             complement(6578..7273)
                     /locus_tag="ACHA34_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFM8792004.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACHA34_001353"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACHA34_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFM8792005.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ACHA34_001354"
     CDS             complement(8472..8663)
                     /locus_tag="ACHA34_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFM8792006.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ACHA34_001355"
     CDS             complement(8684..9574)
                     /locus_tag="ACHA34_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFM8792007.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ACHA34_001356"
     CDS             complement(9561..10328)
                     /locus_tag="ACHA34_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8792008.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACHA34_001357"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACHA34_001357"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFM8792009.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACHA34_001358"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACHA34_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFM8792010.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACHA34_001359"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACHA34_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFM8792011.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ACHA34_001360"
     CDS             14899..15282
                     /locus_tag="ACHA34_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792012.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACHA34_001361"
     CDS             complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACHA34_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFM8792013.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ACHA34_001362"
     CDS             complement(16226..17044)
                     /locus_tag="ACHA34_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFM8792014.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACHA34_001363"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACHA34_001363"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM8792015.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACHA34_001364"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACHA34_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFM8792016.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="ACHA34_001365"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="ACHA34_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFM8792017.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="ACHA34_001366"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="ACHA34_001366"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFM8792018.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="ACHA34_001367"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="ACHA34_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFM8792019.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="ACHA34_001368"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="ACHA34_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFM8792020.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="ACHA34_001369"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="ACHA34_001369"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFM8792021.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="ACHA34_001370"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="ACHA34_001370"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFM8792022.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /gene="rpsF"
                     /locus_tag="ACHA34_001371"
     CDS             27086..27385
                     /gene="rpsF"
                     /locus_tag="ACHA34_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFM8792023.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="ACHA34_001372"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="ACHA34_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8792024.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="ACHA34_001373"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="ACHA34_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFM8792025.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="ACHA34_001374"
     CDS             28352..30325
                     /locus_tag="ACHA34_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFM8792026.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="ACHA34_001375"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="ACHA34_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFM8792027.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="ACHA34_001376"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="ACHA34_001376"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFM8792028.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="ACHA34_001377"
     CDS             33204..34496
                     /locus_tag="ACHA34_001377"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFM8792029.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10554 a         6733 c         6844 g        10505 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaa
//
LOCUS       DAYYFU010000024        34330 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000024 DAYYFU010000000
VERSION     DAYYFU010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.024"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            202..1077
                     /locus_tag="ACHA34_001378"
     CDS             202..1077
                     /locus_tag="ACHA34_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFM8792030.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="ACHA34_001379"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="ACHA34_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="ACHA34_001380"
     CDS             complement(1642..2973)
                     /locus_tag="ACHA34_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8792031.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="ACHA34_001381"
     CDS             complement(3122..3835)
                     /locus_tag="ACHA34_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8792032.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="ACHA34_001382"
     CDS             4010..5434
                     /locus_tag="ACHA34_001382"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8792033.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="ACHA34_001383"
     CDS             5733..6440
                     /locus_tag="ACHA34_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8792034.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="ACHA34_001384"
     CDS             6427..6750
                     /locus_tag="ACHA34_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8792035.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="ACHA34_001385"
     CDS             complement(6839..7876)
                     /locus_tag="ACHA34_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8792036.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="ACHA34_001386"
     CDS             complement(8197..9180)
                     /locus_tag="ACHA34_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFM8792037.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="ACHA34_001387"
     CDS             complement(9595..10365)
                     /locus_tag="ACHA34_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8792038.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="ACHA34_001388"
     CDS             complement(10358..11293)
                     /locus_tag="ACHA34_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8792039.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="ACHA34_001389"
     CDS             complement(11609..13258)
                     /locus_tag="ACHA34_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8792040.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="ACHA34_001390"
     CDS             complement(13375..15978)
                     /locus_tag="ACHA34_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8792041.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="ACHA34_001391"
     CDS             complement(15990..17039)
                     /locus_tag="ACHA34_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8792042.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="ACHA34_001392"
     CDS             complement(17065..17379)
                     /locus_tag="ACHA34_001392"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFM8792043.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="ACHA34_001393"
     CDS             complement(17395..17841)
                     /locus_tag="ACHA34_001393"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM8792044.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17842..19764)
                     /locus_tag="ACHA34_001394"
     CDS             complement(17842..19764)
                     /locus_tag="ACHA34_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8792045.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(19976..20872)
                     /locus_tag="ACHA34_001395"
     CDS             complement(19976..20872)
                     /locus_tag="ACHA34_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8792046.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(20865..21545)
                     /locus_tag="ACHA34_001396"
     CDS             complement(20865..21545)
                     /locus_tag="ACHA34_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8792047.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(21546..22328)
                     /locus_tag="ACHA34_001397"
     CDS             complement(21546..22328)
                     /locus_tag="ACHA34_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8792048.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(22321..23238)
                     /locus_tag="ACHA34_001398"
     CDS             complement(22321..23238)
                     /locus_tag="ACHA34_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8792049.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(23402..23725)
                     /locus_tag="ACHA34_001399"
     CDS             complement(23402..23725)
                     /locus_tag="ACHA34_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792050.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            24513..27740
                     /gene="fss3"
                     /locus_tag="ACHA34_001400"
     CDS             24513..27740
                     /gene="fss3"
                     /locus_tag="ACHA34_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM8792051.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27853..28167
                     /locus_tag="ACHA34_001401"
     CDS             27853..28167
                     /locus_tag="ACHA34_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8792052.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            28180..28554
                     /locus_tag="ACHA34_001402"
     CDS             28180..28554
                     /locus_tag="ACHA34_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8792053.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            28564..28899
                     /locus_tag="ACHA34_001403"
     CDS             28564..28899
                     /locus_tag="ACHA34_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFM8792054.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            28982..30322
                     /locus_tag="ACHA34_001404"
     CDS             28982..30322
                     /locus_tag="ACHA34_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8792055.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            30400..31074
                     /locus_tag="ACHA34_001405"
     CDS             30400..31074
                     /locus_tag="ACHA34_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8792056.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            31067..31231
                     /locus_tag="ACHA34_001406"
     CDS             31067..31231
                     /locus_tag="ACHA34_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792057.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            31307..32449
                     /gene="dcm"
                     /locus_tag="ACHA34_001407"
                     /pseudo
     CDS             31307..32449
                     /gene="dcm"
                     /locus_tag="ACHA34_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            32439..32729
                     /locus_tag="ACHA34_001408"
     CDS             32439..32729
                     /locus_tag="ACHA34_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792058.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            32988..34172
                     /gene="mobT"
                     /locus_tag="ACHA34_001409"
     CDS             32988..34172
                     /gene="mobT"
                     /locus_tag="ACHA34_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8792059.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            34169..34306
                     /locus_tag="ACHA34_001410"
     CDS             34169..34306
                     /locus_tag="ACHA34_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM8792060.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT        10701 a         6609 c         6254 g        10766 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt
    17941 ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga
    18001 atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg
    18061 cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga
    18121 tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac
    18181 caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa
    18241 aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg
    18301 tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac
    18361 aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct
    18421 atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt
    18481 atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac
    18541 tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc
    18601 gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac
    18661 acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat
    18721 atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta gattattgat
    18781 caattcttca tcaggataaa attcatttaa tttaattctt actaactctt gtagctcaac
    18841 acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag catttttttc
    18901 aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa taattgaaaa
    18961 tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt cttcagaatt
    19021 attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag caaggtggat
    19081 agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca tgtatcgatt
    19141 aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg cttcaccaat
    19201 gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa agccacccca
    19261 ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag ctttgatgcg
    19321 cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt ttctaacatc
    19381 tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa gattttgaat
    19441 ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac ggtcctccgg
    19501 agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt ctttttttcc
    19561 aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca ttttttggat
    19621 tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat ataattgttc
    19681 tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg
    19741 ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta agtgtacaat
    19801 tattatctct attttcatac agaagaaagt tcttgattaa tgtagaagtt tctaatattc
    19861 ttgattattt caaatcaata aaaaagcctg caatatttat atcacaggct tttttctcta
    19921 ttaaatatat ataagataat acatttttct actagttctc actaatcttg tataattaaa
    19981 ccctccgcaa cttgacggag atggagaaga tgcccccgtc taatctagcg gtcatctctc
    20041 ctcccattcc ttccaccaat ttttttgaca ggtaaagccc gattccgcta gattccgtat
    20101 ttgtgcggct ggtattttcg gtataaaaac gctggaagac tttttcaaca gaggtttgcg
    20161 ttcctggttt gataggattg gccactgtta atcgaatgaa tggatcctct tttgaaaatg
    20221 tcacctttgc ttgtccatct gcataacgca ataaattctg tatgatattt tgcagcactc
    20281 gcagcaacat gagcggatct gctaatacag ttgtatttat cgagtgttca gcgaacgcta
    20341 cctcgatatt tttctcagaa aattgttcat aaaaagagaa aaatacttct tccacctctt
    20401 tagaaaaaga aagcgtatgg agatccattt ctttttgctt cgtctctatt cgagcaagat
    20461 cataaaactg atccgtcata ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat
    20521 acttctcccg tttctccgta tcctctacct gttccagtaa ttgtacataa ccgttaatag
    20581 cagtcaaagg agttctgaga tcatgagaaa ggccgacgat cgatagtctg atgttttctt
    20641 ccatttcttg aaatgcttgt cctttttgtt cattttcatc caaccatgta ttcagtgcat
    20701 ctactaggtc cattagattc ttttctcgaa attcgactga aagacgacca ccatattctt
    20761 gtcgttcaga aagagtggcg attttcttag ttaatctctt gatttccctt tgcaggaaga
    20821 aaataaaact tcccataaag ataacaacga gaagcaacag ccatttaatc aaatctaaac
    20881 cccactcccc acaccgtatg aatatacaca gcatgtgcct ttgctagctt ccctcgaaga
    20941 ttggaaatat gcatattcac tgttttctca ctatcaaaat atggttcttg ccaaactgtt
    21001 tcataaagat ttgctcgcga aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga
    21061 tcgaattctc ttcctgtcaa atggacagcc acaccattca catttacatc cctcgactct
    21121 ttgtccagta agatttcctt gtaatagatg cgttgcgaca gatcttttac ttggacagaa
    21181 gtctgatgac gaagttggat gccgattcgt aacaaaagtt cttcgatatc aaatggtttc
    21241 gtcacatagt catccgctcc tgctgccaac aaggaaactt tatctctttg atcgcttttt
    21301 gctgaaagaa cgatgatcgg aacattcgat gtctgtcgga tttctgctaa caatgcttca
    21361 cctgaaagcc ctggaagcat gagatctaga aggatcaaat cgacaggata tgtgcttaac
    21421 aataattttc cttccgtacc tgaataagca tcaagaagtt tgtatctctg ttcaagaacc
    21481 tcctccagta aggaatgaac agcttcatcg tcttcgataa tcaaaatagt ttccagcagg
    21541 accacctact taatgtcaga tttttggaaa ataaacattc ctagagctga tgataccacg
    21601 attgttacta gactgaccag aatcattttc ggtgcttgcg aataagttgc ttgttgcgca
    21661 aagtccgtat acatgagcca cgtttccgta aaccactccg ttagaaattc caagcctttg
    21721 atttttgcga aaacaccggg aatgaaggcc gttcccatga tataaagaaa agaaagaagc
    21781 aaactgacac tggtacttct agtgatgaat gtcaaagcca ctggaacaga taatgtccct
    21841 aatgtcagca gaagcaatac aggaaatact tttagaacat aatttccata gtttttgatt
    21901 ccctcaactt caccaaatcc gaaagcaagt gtgtaagcaa gcatggctag tcccgtgatc
    21961 aaaaccacgc caagtgttgt gatcaaaagc gtaacgagaa gtttcgacac ataataagtc
    22021 attcgattcc gtcctaaaga taacgtattg cgaatcgtac cgttttgaaa ttcttcccca
    22081 atataaaagc acgcaaatgc tgcgaaagcc agaagaatcc atgcagccat tttttctacc
    22141 attaaaatcc ctgttgcacc gttgatgact gtatccgttg cttggtttaa ttcattgccg
    22201 tattttgtat gcatatacca ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt
    22261 cctagtgaga cccacatttt acggctcttc catactttta ttttttcagc agttaataga
    22321 ttaaacatgt ggattgcctc cagttaattg taagaaataa tcttccagtt tttgcccaac
    22381 taggtgaata ccatccacag gaacttttgc ttctaccaat gcttgattga tggctgctac
    22441 ttggtcaatt ccttcaaaaa gctggataac acggctagat tgtacaaaat aatccctata
    22501 accaagagag tcgagaaccg ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa
    22561 ttgacgagat tcttgtgcca attcttcttt cgataattct ttgatcaagt gtccttcatg
    22621 caaaatacca tagtgagtgg cgatctgtga caattcatct agcaagtggc tagaaagcaa
    22681 gacggtgatg ccccgttctt ttaccaaacg ttggatgatt tctcgcattt ctgccatccc
    22741 tgctggatcc atcccattcg tcggttcatc caaaatcaaa aattctggat tcgtgatcag
    22801 tgcatttgca atagctaaac gctggcgcat acctaaagag aaattctttg ctttcttttt
    22861 ccctgtattt tccaatcgca tcattttcag caggtcttcg atttctctat ccgacacacc
    22921 gccgttcgct gcttggatac gtaggttatc tcttgcagtt aattctggat aaagagcagg
    22981 ggtttcaatc gattgaccca tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa
    23041 ttcgatcgaa ccttcatcaa tcgagatcaa acccataata gttctcataa aggtagactt
    23101 acccgctcca ttttccccaa tcaatccata gatcattcca cgtttgattt cgattgatac
    23161 tttatctaat gctttggcag caccgtattt tttggttata ccagttgctt tcaagaccgt
    23221 ttcatttcca aaatccatac ttatccttct ttcttattga tgagtgaagt atagttgacg
    23281 ggtataaaga aagcagccag aaatgtataa agaaagtgta aattttccaa ttgaatgtca
    23341 aaaaaaacaa tcggacaata cgtgtctttc ctattatcag gcagtttgtt atacgcttgt
    23401 atcagtttcc tgattctttc ttttcgacca aagtcgtttc tctgtattcc tcttcactga
    23461 aaatttttgc ttctttcaga tcaagcttga gtaattttac cagaccggtt ataaggataa
    23521 ctatagcaag actccctccc agaaaaatta gactgtctcg cagacgtggt tgcgttgtct
    23581 tccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc atggtaatcg
    23641 ttgttatcgt tgacataagt cgtccttgta tatgcgttgg aacttctcgc tgaagccaga
    23701 ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa aaataataca
    23761 tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag acttttcaca
    23821 atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    23881 ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag ctgaccactt
    23941 ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat ctttttcccg
    24001 tttgtttata gatcgaatga tattatagac caagaatcct acctattaac atagctaaaa
    24061 acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga ttaaggtaac
    24121 tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg aacgataatc
    24181 gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct ggttttttct
    24241 tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg taaatatatt
    24301 ttattatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta atttaaaggc
    24361 agtatatttc gtattttaga ttaaattttt aggctcattc attctcaaat ccattctatt
    24421 cagtcaataa aaattttaaa aagactttat aaacaaagat gttcaagcga gcatcttatt
    24481 tttttgtgct taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa aagaaattat
    24541 gtgtattaac tattttgagt ttggctgttc aagcaattgt tggagcaatt gctcctacga
    24601 ttacttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta gatttagcac
    24661 atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa gtcactgttc
    24721 cgcattatgc agtgtacaac ggctttaaac aagatatttt ctgtattgaa ccaggagtcc
    24781 cgatttacaa tgagttcact cctggctatg agaaaaaccc gttaccagat atgtccgata
    24841 aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat actcatattg
    24901 tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca ataaaacgat
    24961 cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa gccattgacg
    25021 attatcagaa gaaaccaacc ttccataata gtacggctaa aacagtatta gggcaatcga
    25081 ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg
    25141 ccgcaaacat tgattatcgt gtgaatggta atcagttgat aatcacccca aatgccaatt
    25201 caaaagaaag tggtgtgctg acgcttaaga aatctgctgg taccggaaca cctgtcgcct
    25261 ataaaaaggc tggccaacag actttaatgg ccggggcgat tgataaacct aatacctaca
    25321 caatcaaaat tgatgtggaa acggagggtt ctttaaagat caaaaaagtc gataaagaat
    25381 cgggtgatgt tgtaccagga acggttttcc atttagactt tggaaaaact ttacctgcaa
    25441 aagacgtgac tactgacaaa gaaggcattg ctacattgga tgagattcct catggtacaa
    25501 aagtaaccat taccgaaaaa tcagtaccgg cgccttatac gattgatacc acccccatga
    25561 ctaccaccat taaagcaggc gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag
    25621 ggcaaatcat tctggataaa agcggagttg aaacagggag tgatctttgg aatgacaatt
    25681 attccttagc tggtaatacg tttgccattc gcaaagacag tccaaccggt gaaatcgttc
    25741 aagaaatgac cacggatgaa aacggtcatg cggaaacacc aaaagagatt gctaatgcgt
    25801 tggaattggg aacctattat gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt
    25861 tcaaacctgt aaaagtcgaa ttgaagtatg ccaatcaaac cgtagcccct gttaccagta
    25921 acgtaaaagg gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag
    25981 atactgggga taaagcacaa ggtaaggcag tctttgaggg tgctgaatac accttgttta
    26041 ctgctaagga tggaaaagct gttaaatgga gtgaggcttt taaaccagag ttagcgaagg
    26101 gaacgaaagg ttctgatgaa acggtcactt tgactttaga cgaaaagaac caagttgccg
    26161 ttaaacattt agctatcaac gagtattatt ggcaagaaac aaaagcgccc gaaggatata
    26221 gccttgatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg
    26281 ccgtgattac ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc tttgatttct
    26341 ttaaatttgc tggatcagct gcgggcacta ccgaaactgg cttcaatgat ctgaccttta
    26401 aagtgtcgcc attggaaggg accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag
    26461 cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt ccttatgggg
    26521 attatttact tgaagaagta gaagccccag aaggttttca aaagattacg ccattagaaa
    26581 tccgttctac ttttaaggaa aacaaagaag actttgtgaa gagtgagtat gtctttacca
    26641 ttaccgaaca agaccaaaaa caaccaatta aaacggtgac cgttccttac gagaagctga
    26701 cgaacaaagc attttctgtt agtttgaacc gtttaatgct ctatgatttg cctgaggaag
    26761 aagatagttt aacttctctt gcgacttgga aagacggaaa caaagaatta acttctctgg
    26821 attctaccga gctagttgat aaattgagtt acaatctaca tgaaatcaaa gaagactggt
    26881 atgtggtagc gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa
    26941 aagccaaacc agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag
    27001 gaacttggaa aattcagcat aaattaaccg ccgaacaagt tttaaataaa actattgtct
    27061 tattcaacta tgtgtatgaa aataaggaag cttttgaagc aggcgataag ccagtagcga
    27121 aggatgttag cttgaataac caagcccaaa ctgtcagttg tacagtggaa caccatgttt
    27181 ctatccaaac aaaggcccac ctagaaaacg gttctcagac ctttactcat ggtgatgtgg
    27241 tggatatgtt tgatgatgtg tcgattactc atgatgtact ggatggctca aaagaagctt
    27301 tcgaaacaat tctgtatgct ttattaccgg atggtacaaa caaagaaatt tggaaatctg
    27361 gcaagattga ttatgaagta aatgataaag aattcaccaa aactgtgctt gcgaaaaaag
    27421 tagataccgg aaaatatcca gaaggaacca agtttacttt tgcggaaatc aattacgata
    27481 aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct caaaccttaa
    27541 caccaaaaga agttccaacc atactaagta cgccgaaaca accggaaaca ccaactgttc
    27601 caagtggctc tcaagaatct agtcccacag tgaagacctt ccctcaaacc ggggagaaaa
    27661 attccaatgt tttactgttc attggtttta ctttgatttt tgcgacggcg ggctattatt
    27721 tctggaatcg ccggaactaa ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg
    27781 cgcgaccgcc tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa
    27841 gaggaaaaga agatggaatt gaaatttgtc gtgccagata tggctgaaac ctttggaaag
    27901 ttaagttatg ccggtgaagg agaaattata acagaaggat atggacggaa tactacggtg
    27961 attggtcgca gttatcattt gtattccagt aagcaacggg ccgatgatat tgaggtggta
    28021 gtagctgcgg aagccggtga aaaggatttt gatcaagacc aaccgctaaa agccgtgaat
    28081 ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttactga ttacgtgttg
    28141 tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac agaaggtatt
    28201 gtcgtagatt caggtttaac atttgggaag ttgcgttttt ccgcattacg ccgagaagta
    28261 cggaagcaaa atgaggatgg aacaattggt aatgaggtaa aagaacgaac ctataattta
    28321 aaatcctctg cacaagggcg aatgattcaa gttagtatcc cagctaatgt acccttacgc
    28381 gagtttgctt atgatgcaga ggtagagttg gtcaatccaa tcgtggatac cgttgccaat
    28441 tatgtttttc gagaaggaac taccgttaat tggttcatta aggcggatga tttagtcttg
    28501 aagcggcagc cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag
    28561 attatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg aaaaggcgag
    28621 ctgactgagg aacaactcgt taaagcatta acctctgatg aaaaaatgtt tctgattata
    28681 catcaaggtt tacttggaga aaatagcgac agtgaagaag actctgatgt actagactgg
    28741 ttaggagaag aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag
    28801 gcagaatcca ttttggaaga aattggtgtg gagatgccgg atgctattga aggcttttta
    28861 aatcaattgg ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac tagcataact
    28921 ttttgaaatt gcctaatgaa atcctattgg gctttttcga ttggaggtgg aaaccatcaa
    28981 aatgtacaaa ggacatcgca tacgcgcagg agatcagcac ttggtttatc actttgttct
    29041 tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc aagagtttag
    29101 acaatttgat atttctaagc tatcactttc tactattgaa atcgtttggt ctataaaaga
    29161 tttagtatgt ctgcttggaa gtctcgcttt ttcaggagct atgatattgc tttatattca
    29221 cttttttctt gatcattggc gaagcttgtg gcatcgacaa aagctagctc ggatgattct
    29281 agagaaccat tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa
    29341 tagtagccga accagagaga ctatcagtta cttccccaaa atctgttatc gaatgaaaga
    29401 tggattactg tctattcgcg ttcaaatttc acttgggaaa taccaagatc agcttttaaa
    29461 attggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg aactcaaaga
    29521 ctcctatgtg gagtatactt tactgtatga tatgatcgcc aatcgaattg ggatagacga
    29581 agtagtggca gaaaatggga ctctgcgatt gatgaaaaat caagtatggg cttatgattc
    29641 tttaccccat atgttaatcg ctggtggcac gggtggaggg aagacctatt tcctcctcac
    29701 cattatcgaa gcactattga agtcagatgc agaattgttt atcctcgatc ccaaaaatgc
    29761 agatttagct gatttaggta cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc
    29821 tgcttgtgtg gaagatttct atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat
    29881 gtctaactac aaaacaggag agaattatgc ctatcttgga cttccaccca actttttaat
    29941 ctttgatgaa tacgtggctt atatggagat gttaacgacg aaagaaagtg cggtgatttt
    30001 gaataagctc aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc tgattttggc
    30061 ttgccaaagg ccggatgcga aatatttagg agacggaatc cgcgatcaat tcaactttcg
    30121 cgtggctttg ggtcgtatga gtgaactcgg atattccatg atgtttgggg aggtcgaaaa
    30181 aaacttcttt atgaagcaca tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat
    30241 tagtgagttt tatacgccac ttgtaccaaa agggtatgat ttcttagatt caattagtaa
    30301 attagaaaag atgaatgatt gagtgatctt caatactgac tttatacttc aagtaacgta
    30361 taatactttt atatagtagg aagaaagggg ataattaata tggaaagtaa tgattcttat
    30421 ttagagtatg caaaagcaaa taaggataag tttatttctt atattatcaa agggaaatcc
    30481 cccgaagttg gacgagacgc agtttttatg gcagggagtc ctggggctgg aaaatcagag
    30541 gttgccattg gattagctga gaattatgat aaccatgtga ttattgatgc ggattacttt
    30601 cgcacccagt ttcctgatta taatggaaaa aactctagtc tctttcaaaa agcatcttct
    30661 tggttggtag agcaggcatt tagatatgtc ttagatcatg gctattcttt tattctggat
    30721 gcaacttttg caatattaag tgctgaaaaa aatatctcgc gggcgttgaa aaaacatttt
    30781 cgagtgacga ttttctatgt ttatcaagac cctaaaatcg cttggaaatt tacacaagaa
    30841 cgtgaaattg cagagggacg tcatgtgccg aaaaaggcat ttattaacgc ttttttcaaa
    30901 tcaagagaga acattgcaaa agtaaagagt aaacatccag atgtattgct tcatatcata
    30961 ataaaagatt atacgaataa tatttccgaa gttcattatg ctacggataa tctagaatta
    31021 tttttaccac tagggtatag taggaaagat ttggaggttc aattacatga ctgagaaaga
    31081 acaagtaacg aaaattgtaa agaaatacaa taagagcatt gcggatttat cggaaaatgc
    31141 tactgcaaaa gagttcaaaa cggtgattaa atatgtagcc gatcaggcca atgaaaaaca
    31201 acggaaatta gttggattaa ataaaaaata gtaggacaaa ttaataatgt atgagccaag
    31261 agcaaaatgt tcttggcttt tttctttgca atgaatgagg taatgtatga gatttttaga
    31321 tttattcgcc ggtattggtg gctttcgttt aggtatggaa caggcgagcc atcattgtat
    31381 cggtttttgt gaaattgata aatttgctag acgtagttat aaagcaattc atgatacaag
    31441 taaggaggtg gaaatgcatg acatcacaag tgtatcagat gagtttattc aatccctcgg
    31501 accagtggac atcctttgcg gcggatttcc gtgccaagct ttttcaattg cgggaaagcg
    31561 gcaaagattt tccgatactc gaggtacttt attctttgaa atcgctcggt tcaccactct
    31621 tctccgacct aagcttttat tccttgagaa cgtccgagga ttgctcaatc acgaaggagg
    31681 ggctacgttc gagacgatcc tccgaacgct ggatggattg gggtatgacg tggagtggta
    31741 ggtgcttaac tcaaaggcct acattcccca aagccgggag cggatattcc ttatcggaca
    31801 ttctcgagac gcatgtaccg aacaagtatt tcctatcatt ggaccgtctt caacatctga
    31861 tcaaaacatc agaaatttgg taaatatcaa tccttccaat cgaggaatgg gtggacaagt
    31921 ctatggttca gatggcgtag cgccaacctt aacaggtgat gaaggaatca aaatcgcact
    31981 gccagcaagg gatggaatct ctgtagcagg actattaccg ggaaagtttg aacaaggtaa
    32041 tcgagtttat gaagttactg gaaccgcccc caccttgtca acgaaacaag gtggtacaaa
    32101 aatcatgatt cgaaaaaatg aaacagaata tcaagaggtg aagccagaag acagtgtgaa
    32161 tctggctttt cctaattcca caagtcgccg aggtcgtctg ggaaaacaga gtgttcatac
    32221 cttactaaca ggagaccaac aagcgatcgt cacggatcag tatcaaattc ggaagctaac
    32281 acctagagag tgttggcgac ttcagggctt tcccgattgg gcttttgatc gagattctca
    32341 agtcaattca gatagtcagt tgtataagca agccggaaac tctgtaacag ttccggtcat
    32401 ttttgatatt gccagaagat taaaagagtt ggatgtacat ggtttatgat gagttgattg
    32461 gagaaattta ttgggtgatt ggaaagattc agtcagaccc agaattagaa gaacaattgc
    32521 gacggttaaa atttgatatt cgcaagaatg gtgtcaaagt cgctggcgat ccatatttaa
    32581 tgaatgagga gacggatgct cgaattgaaa taaatcaagt aattgcagaa tttgagcgga
    32641 ttgcggacca agcaaaagaa ccagatattc gtcaatactt gttagaaatg aaagcagagt
    32701 tggaagtaaa tggtattaca gatgagtaac ctaaaccaac acaactgact gttaggcgct
    32761 gaccgcggag gattcgcgaa gcagaaatcc tccgtggtgc tgtgcctgtt catggacggc
    32821 gttagccgac catgatgtct cacccccgta tctaacaggg gggtacaaaa acacaaaaaa
    32881 taagtaagca accattaaga atgcttgttg tatcaaagat agtaagtaat ttgtataggt
    32941 gttaactcat ttgaaaaagt taacaccttg aatttggagg tgagaacttg gcacaacgga
    33001 atttagatta tcggctatta aaagaccgac gcaacgaata cggcgtttca caaaataaat
    33061 tggctacggc ttgtggactt agtcggccgt acttaaatca gatagaaaat ggtggcgtaa
    33121 ccgcatctat aaaaaccatg aggaaaattt ttgaccaact ggaaagcttt aatcccgatt
    33181 tgcctttgac gttactgttt gactatgtaa ggattcgctt tcccacaacg gatgcacgaa
    33241 aaattattca agaaattctc catttgaaat ttgagtatat gctccatgaa gattatgcct
    33301 tttactccta tcaagaacag tatgtcatgg gtgatattgt ggtgatgttg tctcatgaag
    33361 aagataaagg tgtgctttta gaattaaagg ggcgaggttg ccgacaattt gaaacttttt
    33421 tactcgctca aaagcgtagc tggtacgact ttttcgagga ttgtctaaag gccggtggcg
    33481 tgatgaaacg cttggacttg gcaatcaatg atcgagtggg gctattggat atacctgagc
    33541 taacgaaaaa atgtcagaag gaagaatgta tctccttgtt tcgtaccttt aaaagttatc
    33601 gttccgggga acttttaaag gctgatgaaa aggacggcat gggcaatacc ttatatatcg
    33661 gcagtctcaa gagtgaggtt tatttctgtt tgtatgaaaa agactatgaa caatatatca
    33721 agttagggat tccattagac caaactgaga cgaaaaatcg ttttgagatt cgactgaaaa
    33781 atgaccgtgc ttttcatgcg attcaagatt tgttaaaagg tcgtagcatt gagagcacca
    33841 cgttttccat tatcaatcgt tacttgcgat ttgcggataa agtagaaggt aaaaggcgaa
    33901 ccaactggcc cttgaatgaa caatgggggc gttttattgg acggaatcgg aaagagattc
    33961 aattaacttc cgagccaaaa ccgtatacca ttgaacgaac cttgaattgg ctaggccgac
    34021 aagtcgcccc tacgtggaaa atggctaagg agttggatcg attgaatcgg acaacctata
    34081 tccaggatat ggtacagaat gcacgcttat cagaccgaca taaaaagatt ttggagcaac
    34141 aaagtatggc aattgaaaat ttgattatat gaaaagagga atcgtcatgg gaattgtaaa
    34201 agatattttt ttggtatttg gtggcagtat gcttggtgtt actgtgatgt gcttgatgaa
    34261 tgcaagtgca gcagcggatc gagcaatgga aaagaaagga aagtgacaag atgaatttcg
    34321 gacaaaattt
//
LOCUS       DAYYFU010000025        33682 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000025 DAYYFU010000000
VERSION     DAYYFU010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.025"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..2383
                     /locus_tag="ACHA34_001411"
     CDS             56..2383
                     /locus_tag="ACHA34_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFM8792061.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2380..3294
                     /locus_tag="ACHA34_001412"
                     /pseudo
     CDS             2380..3294
                     /locus_tag="ACHA34_001412"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            3367..3882
                     /locus_tag="ACHA34_001413"
     CDS             3367..3882
                     /locus_tag="ACHA34_001413"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFM8792062.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            3879..4325
                     /locus_tag="ACHA34_001414"
     CDS             3879..4325
                     /locus_tag="ACHA34_001414"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM8792063.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4475..5290
                     /locus_tag="ACHA34_001415"
     CDS             4475..5290
                     /locus_tag="ACHA34_001415"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8792064.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5496..6668
                     /locus_tag="ACHA34_001416"
     CDS             5496..6668
                     /locus_tag="ACHA34_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8792065.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6830..7537)
                     /locus_tag="ACHA34_001417"
     CDS             complement(6830..7537)
                     /locus_tag="ACHA34_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792066.1"
                     /translation="MKKLVALFLVAGSLLSGCGATTSTTQSTSSAQASSSSTTISEST
                     ADSSKEQIEKKKEEEKQKAEKAALEKKVTEAESALSQAEANPTKENYATASAAIQALP
                     ENKQELTSRLAAIDSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAI
                     AQQQAEQARQQAAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATL
                     SEAQARGLTPCSKCYGN"
     gene            7907..8329
                     /locus_tag="ACHA34_001418"
     CDS             7907..8329
                     /locus_tag="ACHA34_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8792067.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(8495..9688)
                     /locus_tag="ACHA34_001419"
     CDS             complement(8495..9688)
                     /locus_tag="ACHA34_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8792068.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            9912..10289
                     /locus_tag="ACHA34_001420"
     CDS             9912..10289
                     /locus_tag="ACHA34_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8792069.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            10277..10615
                     /locus_tag="ACHA34_001421"
                     /pseudo
     CDS             10277..10615
                     /locus_tag="ACHA34_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            10749..11192
                     /locus_tag="ACHA34_001422"
     CDS             10749..11192
                     /locus_tag="ACHA34_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFM8792070.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            11453..12268
                     /locus_tag="ACHA34_001423"
     CDS             11453..12268
                     /locus_tag="ACHA34_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8792071.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            12278..12940
                     /locus_tag="ACHA34_001424"
     CDS             12278..12940
                     /locus_tag="ACHA34_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8792072.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            13148..14812
                     /gene="alsS"
                     /locus_tag="ACHA34_001425"
     CDS             13148..14812
                     /gene="alsS"
                     /locus_tag="ACHA34_001425"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFM8792073.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            14825..15535
                     /gene="budA"
                     /locus_tag="ACHA34_001426"
     CDS             14825..15535
                     /gene="budA"
                     /locus_tag="ACHA34_001426"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFM8792074.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            15665..16162
                     /locus_tag="ACHA34_001427"
     CDS             15665..16162
                     /locus_tag="ACHA34_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFM8792075.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            16347..16607
                     /locus_tag="ACHA34_001428"
     CDS             16347..16607
                     /locus_tag="ACHA34_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792076.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(16784..16912)
                     /locus_tag="ACHA34_001429"
     CDS             complement(16784..16912)
                     /locus_tag="ACHA34_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792077.1"
                     /translation="MQPLQMLYDNKPELNDPDISFAFFEMIGSDDLFKTTSLSIRK"
     gene            complement(16969..17208)
                     /locus_tag="ACHA34_001430"
     CDS             complement(16969..17208)
                     /locus_tag="ACHA34_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792078.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            17494..17808
                     /locus_tag="ACHA34_001431"
     CDS             17494..17808
                     /locus_tag="ACHA34_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792079.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(18242..18608)
                     /gene="ssrA"
                     /locus_tag="ACHA34_001432"
     tmRNA           complement(18242..18608)
                     /gene="ssrA"
                     /locus_tag="ACHA34_001432"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(18779..19552)
                     /locus_tag="ACHA34_001433"
     CDS             complement(18779..19552)
                     /locus_tag="ACHA34_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8792080.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            19696..20046
                     /locus_tag="ACHA34_001434"
     CDS             19696..20046
                     /locus_tag="ACHA34_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM8792081.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            20027..20743
                     /locus_tag="ACHA34_001435"
     CDS             20027..20743
                     /locus_tag="ACHA34_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFM8792082.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            20743..22191
                     /locus_tag="ACHA34_001436"
     CDS             20743..22191
                     /locus_tag="ACHA34_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8792083.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(22261..22770)
                     /locus_tag="ACHA34_001437"
     CDS             complement(22261..22770)
                     /locus_tag="ACHA34_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792084.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(22760..23485)
                     /locus_tag="ACHA34_001438"
     CDS             complement(22760..23485)
                     /locus_tag="ACHA34_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792085.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(23708..25828)
                     /locus_tag="ACHA34_001439"
     CDS             complement(23708..25828)
                     /locus_tag="ACHA34_001439"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8792086.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            26058..27236
                     /locus_tag="ACHA34_001440"
     CDS             26058..27236
                     /locus_tag="ACHA34_001440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFM8792087.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            27252..28010
                     /gene="aroD"
                     /locus_tag="ACHA34_001441"
     CDS             27252..28010
                     /gene="aroD"
                     /locus_tag="ACHA34_001441"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFM8792088.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            28033..28518
                     /gene="ybaK"
                     /locus_tag="ACHA34_001442"
     CDS             28033..28518
                     /gene="ybaK"
                     /locus_tag="ACHA34_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFM8792089.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(28589..29842)
                     /locus_tag="ACHA34_001443"
     CDS             complement(28589..29842)
                     /locus_tag="ACHA34_001443"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8792090.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            29960..31309
                     /gene="gdhA"
                     /locus_tag="ACHA34_001444"
     CDS             29960..31309
                     /gene="gdhA"
                     /locus_tag="ACHA34_001444"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFM8792091.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            31421..32767
                     /locus_tag="ACHA34_001445"
     CDS             31421..32767
                     /locus_tag="ACHA34_001445"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFM8792092.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(33075..33461)
                     /locus_tag="ACHA34_001446"
     CDS             complement(33075..33461)
                     /locus_tag="ACHA34_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8792093.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(33510..>33682)
                     /locus_tag="ACHA34_001447"
     CDS             complement(33510..>33682)
                     /locus_tag="ACHA34_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFM8792094.1"
                     /translation="VETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQR
                     RFEGTLRYSLGY"
BASE COUNT        11201 a         5923 c         6705 g         9853 t
ORIGIN      
        1 taaatttaga ataacgtgta aaggattatt tgatgatttt ttattgagag gagggatgaa
       61 aatgattaag aaaactcaaa ataaaatcca acgtatatgt caaaaacatt ttgtttctag
      121 actcaaagct gtatctaaaa gaaaatactt acatcccatt atgatttcga tcatcttgat
      181 ttttactttc ttagggagct tatcaagtat ttatgtaaac gtttatacag aaacgtcaaa
      241 ttttgctaac caaacgacag aggagacaaa tagtccaccc tattctgaaa tacagtccat
      301 agaaagcacc aatcagtcta gcaacaacga gacaacctca accacatcct cgactcaact
      361 gattgatccg acagaaaaag aaatcacagc agcaaaagca gcaggggaga aagcttattc
      421 agaaacaggt agaagccaga catttagtac tgttgcggcc actgcgaatt tcaacggaca
      481 taccatacca gatggatata cagtgatata tgccaaagat ggagtcattg ttgcggttaa
      541 cggatcgatg agtgcggata aaactgcgtt acaaggcgta accaaagcaa tcgcttttat
      601 tgataaccgt ccagatatcc ataatctgtt tattggctcc acagtaaatg gtactactat
      661 cacattccca aaaagtatct atatgggggt ggctaccgct ggaaatacga tcgattcagt
      721 tacacccaat gcggataaaa gttattcttt tacaccctca acatggggag ggcttcctgc
      781 cggaggaggt atgagtactt tttcaatcgg tatacagtta gcaaatgata ttccattcac
      841 ggggtactcg atcaaaaatg ggcagatcgt tgatggtaag ggaaatgttt caaattttcc
      901 aacctatcaa gcagcaacgg ttcaggtcaa tgcgaatcgg acaggaactt tttcttatac
      961 agtcaatggt ggagcagcaa aaatcggaac gtacgggact acattatctg gaatccatgt
     1021 gggtgacaaa gtggttatca ctccaacagc agtaacgggc tattcttttg cacaaacaaa
     1081 cggttctttc acagccctag aaagtaacgt cgatacggtg acctatacag gcaaacaaac
     1141 accaatcact attaagtatc aagactattt tggacagatg attgctcctt ctaaaataat
     1201 gaatcttact tacggaagtg ctgcacaaga cctgacgact aatacgccta ctatcaacgg
     1261 atataccttt acagctgttt cagcgataga aacaaagaac caatcagcaa tgagtgtttt
     1321 agctagttta gatacaaatg gaaatgtaat cgtaaaggac gcaaatggaa aacagatttc
     1381 tgaagtcatc ctatattata agacaaatgc aacagtatca atcaatgcaa atggcagtaa
     1441 gtactatgat ggtctttctg ttgtaccgat cgttaaatat gcctttaatg ataaaacaga
     1501 atcaacatcc cttttaaaca atgatctcag tattgatcaa atcacatgga atccaactga
     1561 tttcaaagcg atgaatgaaa agggagttga agtaactgcg cctactgaaa ttggaaccta
     1621 tacgtcttgg caattgacaa aaagcggact agctaaacta gctgccagaa caaactattt
     1681 atttactatc gtgcagacat ctgatgtcta tacgattaag caaatcagcg gtgcggtaga
     1741 gctcagtggc agtaagacat atgatggaaa agcaggtgtc ccgtcaatcc atgtcaagct
     1801 agcagagggt gtcactagtt cgcttactcc agtacaagtg gcacttagca gtatggatta
     1861 cacagcagat gcacaatcag caaaaaatat ggttaatgct ggcagctata caattaaact
     1921 aacgaatagc ggaatagaca aagtcaaagc agcaaatccc aagttgtcat ttactgatat
     1981 agccaacaca tcaggaactt ataccatcta taaagctaac gcagtgatta ctgtcgatga
     2041 cgctgccttt aattatgatg cccaaagtca ctcgatttcg gttggaaatc tccacgttac
     2101 cggtgttgtc ttaggcgaat cattggatta taccctaaca gttaacagcc ggacagatat
     2161 tggatcacaa atagtaacga ttagtttagg cgaaggaagt gtaaacaata attatacgat
     2221 tatcgcaaca gataccgcta agttgacgat caatccgact ctcttgcttc cttcgacagg
     2281 tgggataggt ctactgccct ttgtcttttt aggactgatc tttatttttt ctggcttctt
     2341 ttattttatc catcgtaaga aaaaagcagg tgaacagcga tgaaaaaaac aaaaaatgta
     2401 cgtgtggata tatttgctgt tctctttttt atcctactgg tcttttttat aggttctccg
     2461 actgtgtcag ctagagaatc acctattaca tttactttgc ataatcgaat gttcgaaagc
     2521 gataagatgc caagtatcag tcaaaacgac ggaataaata tcccggataa ctttcaagga
     2581 gtaaaagggt tgaacggcgt tactttcgct atatatgatg tctcggatga attttacaag
     2641 ctgcggtcag aaggaagtag tgtggaagac gcacaaagaa aactagcaca aaaaagtgat
     2701 agtgaaaaaa tactggcaga aaatgtgaca aaaacagtag acgaggaaga gggcattgct
     2761 tcttttagtg catcggacaa agatgagcaa ggtcgtgacg ctgtttaccg atttacggaa
     2821 ataaagactt cagattaagt aaaagaaaaa tcagcaccat ttgtcgttgt gcttcctgtg
     2881 acagattcat ctaatcataa attgacgacc atccacttgt atccaaagag cgaacaaaaa
     2941 atacctgctg cactcacatt aacaaaaaca gtagagaata agcagacaga ttttgcagat
     3001 ggggataaag tcccttatct catcaccgta acaattcctg agaacatcaa cgagatagga
     3061 acctatacga tcaaagatac agctgatcct cagttatggc tagaaccaga aacgatcgat
     3121 tttttgattg gtggagacaa gattcataca tttcacacac aaaaaataaa acatggtttt
     3181 gtcttatcag attctggaaa agatcttagt tcgtttgctg gaaaaaaatt aacgatccgt
     3241 taccaaatga cattgaaaga aaacagtgaa aaaacgacat ttgataacga tgtacgtttg
     3301 acgactgaca atcaaacagt agaaacacat cttgccatcc aaacaggagg caaacaattt
     3361 gtcaaagtgg atcggcaaac gaaacaaaaa ctagcagatg ctcaatttct agtcaaaaaa
     3421 aatgaccaat acttagcgca agaaaacggg ataaatcatt gggtagccaa agatgattca
     3481 agagcaacga tttttacttc cagtaaagat ggaacatttt ctgtccatgg attgtctttt
     3541 ggaagttacg aactagtgga gattaaaccg cctaaaggat atatattgag tcaatcaacc
     3601 attctctttg aagttgaaaa aggcagttat gatccgcttc atcctatacc attggaaatc
     3661 atcaacgaac caaaagcaaa tccgacgaat aatggaaaaa cgccagttca gatgcagaca
     3721 aatggtccat caaatgaaaa aaatggagga agctttccaa aaacaagcga agagatgctt
     3781 ggaggtttct caattttagg gttattgcta gtcctttcaa cagggacagc ttggttttat
     3841 aaaaaacaac aaaaaggaaa caataggagg gaaataaaat gaaaaacatc aaaacaaaaa
     3901 tatggacgtt tttaggaact gctatcatgc tgcttccttt cgttctcggc ctaggaactg
     3961 cagaggtttc tgcagccgta tcacctacac cagaaaatgt tacggttaat ttgcataaat
     4021 tgaaattcac ttcagcacca gaaaatcaaa taaacaacgg aacggaattg acatttccga
     4081 atagcgaacc gttgaacggc gttgaattca acgtatatga catcactgct acgtactatc
     4141 caagtaaaga cacagcagtt ccagcagacg ctacaccctt cgcaagtgta acgacttctg
     4201 gtgaaggatt agctaacctg actcttccag gtaaatctga tgggaaagat gctgtatacg
     4261 tctttgtaga aacaccaaag cccggtgtcg aaacgtcgcc aaatatcgtg ttaagcttac
     4321 cctagaccct gacaaacctt tagacacgat tcacctatat gctaaaaatg tcatcaaaga
     4381 aagcaatatc aaaatccaga aagctgggaa cttttcaggg gtagataagt tagcaggggc
     4441 gcaattccag atcaaaaatt cagagaatca attcgtgacc ggttttgatg aaaacactgg
     4501 tgtcgctctc tatgatcctg ctgagtcagc gttgacattc acaactgata ctgaaggatc
     4561 catcagtgtc aatggattaa tagacggcaa ttatacttta gttgaaacaa aagcaccaga
     4621 tggttatcaa ttaaccaatc cacaaaaaga aactactttt atagtaaaaa acggaaaatt
     4681 gcaacaaaca accggtatca tccatggcgc agatgaagcc aatcttggga atccaaacgg
     4741 tgtagtcaat cgggaaaata caactacgga taacacgatt actgtagaaa atgtccaaaa
     4801 ctacggtgat ttccacttca tcaaacaaga tgtgaacacg caagaaaaat tagccgatgc
     4861 agaattttct attctaagag caaaagatcc aactgttaaa ttaatgaaaa aaatagcaaa
     4921 ccttgcctca ggagaatacc catatgcttg gtcagatgaa gtcacaggtt ctgactggga
     4981 agaagtgaaa ttgatttctg attcagaagg gaaattttca atcgaagggc tgaaacaagg
     5041 ggcatactgc cttcaagaaa caaaagcacc agaaggctac gcattaccta agaatgaagc
     5101 tgcgtacact gaatttacag tagatgatga tccaacaact tctgatgaaa caacgatcga
     5161 taaccaacct aaaggaatct tgccttcaac tggtggtatg gggatcatcg tattcatctt
     5221 ggtaggtact gctttagttg gaggagcagt gatctatttc aaaaaacgcc atcaagaaac
     5281 agaagcttaa actattggaa acaaaaatca tacgataaat aaacgatagt gattcaaatg
     5341 gggagaaagg ttgtgacaag tggtcggtct cttggtattc gctcaacaaa cggcaaaata
     5401 ggaggaacta ttttgccgtt tgcttcgcta atactcaggt caaggcgctg atttcacaac
     5461 ctcttcatat acatattaga aaataggtgg acatgatgaa taaaagcaga ataagaatac
     5521 tggacatctt catggcaatc attttagtcg ttgggatcgg gatcttttct tatccttttg
     5581 tagaagattc actgaatgat tttcttgccc agcaaatgat catccattat caaaagcaag
     5641 catcgaataa gaattcagag gaaataaaaa aacagcagga aaaaatgaca aagaaaaacc
     5701 aacagttggc agagcaaaac gtatcaccag ggataggcag tttcaaccaa gcagttgatg
     5761 caaaagcatt aaaagattta cctagtaatg ccttttttat ggaacatatg cttggagtca
     5821 tcgaaatccc aaaaatcaat gtcagcttac ctatattcga tcagacaacg gaaatatttt
     5881 tgcaaaaagg aacgtcatta ttggaaggaa gttcttatcc gactggcggg aaaaacaccc
     5941 atgccgttct ttctgggcat cgtggactcc cagaagcaaa attatttaca gatttgccta
     6001 agttgaaaaa aggagaccag tttttcatcc agatcaatgg aaagacattg gcgtatcaag
     6061 tagaaaaaat ccaagtggtc ttacctgacg aagtagattc tttgggtatc caaaaaggca
     6121 gagatctcgt gactttgctt acctgcactc cttatatggt caatacacat cgactgcttg
     6181 tgacggggca tcgtattcct tatcaagcaa aagaggcaaa aaaagcgatc caaggaattg
     6241 atcaatggaa aaaatggaaa ttttttattt ggtttatcgg tatactactt ggatcaatcg
     6301 gcttagtttg gctactcatc gcttatttag attctttggc catcgctaag cgaaactatc
     6361 ctttatcttt ttatgtaaag aacacaaacg gacgaccaat cgaaggaatg gtcttttctg
     6421 taaaaacgct caacggcaaa cactatatta ctagagaaaa agttccattt gtgaaagctt
     6481 ctgatgaata cggactagtg agattttctg atttaaaagg caggaactat cgcttgcaac
     6541 atgaggagct cctgcttaaa atccatgtaa aacataaaca ttcgaaacag ttttcaatga
     6601 aattgaaaaa aggaagatat aaactgcgaa aagaaaaaga ggtgtactat ttgattgaga
     6661 aagaatgaat gaagatgtcc aaaaaagcag agggaacaga ctaaatgaaa aaagagagaa
     6721 gctgacgaaa tggacgtgta aactgctatt tttggtttac tatctggaac gccagcttct
     6781 atttttttat gtttcagaag aattttatta ttatagtata acttttgaat tagtttccat
     6841 aacatttgga gcaaggggtt aaaccacgag cttgagcttc gcttaaagtg gcaggggtat
     6901 acgtaccatt tccacattta tgtgtatggt attttgaacc ggtcggtgtg acatagacag
     6961 tttgttcatt attttgctca ggagctgcgg cttgtgcctg ctgagaagcc tgttcagctg
     7021 ctgcttgctg tcgcgcctgt tctgcttgtt gctgtgcgat tgcttcttgc ctcgcctgtt
     7081 ctgcttgttg ctgtgcgatt gcttcttgtt gcgcttgttc tgcttgttgt cgtgcaattt
     7141 cttcttgctt tttccgttct tcttctgctt cttttgcttt gattgtggaa tcaattgcag
     7201 caagacggct tgtcaactct tgtttgtttt caggtagcgc ttggatagct gctgaagcag
     7261 ttgcatagtt ttcttttgta ggatttgctt cagcttgtga caaagcagat tccgcttctg
     7321 tcactttttt ttctagtgca gctttttccg ctttttgttt ctcttcttct tttttctttt
     7381 caatttgttc tttcgatgaa tctgccgtac tttcggagat cgtggtactg ctgctggaag
     7441 cctgagctga acttgtgctt tgagtagtag acgtagtagc accacaccca ctcaacaaac
     7501 ttcctgcgac caaaaacaac gcaactaatt ttttcactaa atttcctcct taagtaaaaa
     7561 acaaccgttt tcaataaatg tccttttcaa ttgtttactt acttgttata ataattgtgt
     7621 tatgaatagt gattgaggaa acttaatcta atcccgtgtt gatacacggg attttttgct
     7681 atagttctgt tattaagtat gcaataataa aatatgtacg ttattttatt attagattat
     7741 tgacatttat ataataaacg attaaatgat tcatatcaat agaatactta ggaaaaaaag
     7801 agaaataata acaggtttat gcactattga cggtgtgaca atatgatatt tgtatccgtt
     7861 tttttcttgt atgtagtata gtaaagtaga tacaggagga gaaaatatgc agactttttt
     7921 aacaaaagtt tctcaaaaga aaattaaaat gattcattta ctgcttgaaa cagaacgttg
     7981 gtgttcaata gaagaactac aaaacgaatt gaaggtatca agtaagtcta tcttgaatta
     8041 tctaacagat ttagaagaat tgttccaaca atatcctgat aaggtcattt tgaagaatga
     8101 gaataatcgg cgcttttcta tggaaaagca ggagaacttc ccaatctaca ttatttatct
     8161 tcatttctac agaaaatcat ataattatca tttgattgaa ttcatgtatc aacatcctga
     8221 gaaaaatttg gaagattatg cagacgctca atatacgagt gtatccacag tttttcgtta
     8281 tgctaagtta ttagtaaagt actttgaaat gacggacgtc aaagaatagt cgccgccaga
     8341 tagaaggaaa ttagcagttg aaaaagctgc cctgcatcaa gtaatacgac cagccaaacg
     8401 gaaacagtgt ctcatgtttt tgatttggct ggtcttatta ccgcagatgc aagctttttc
     8461 cacacctctt tcttgtcatt tacagactca actatcaatg ctgcaagctt tcccttaatg
     8521 ctgtaacacc agtaagagtt gtcaagacca gcagactgac taggtaaata ccgctcaaaa
     8581 acaagattac ggcagttaaa ctataatgat ccatcaagaa accgatgacg atagaagaaa
     8641 aaccaccaac agctcgtcca aggttcataa cgaaattatt cgccgtcgcg cggatttctg
     8701 ttggatacaa actactaatc actgcgccat atccaccata catgccattg ataaagtagc
     8761 cgactatcac agcggctgct accaaactcc actggctatg ggctaacaca agtagataga
     8821 cagataagga tgaacaaatt aaaaacaaag aaaaggctag tttaggcccc aatttatcca
     8881 tgattatgcc aaaagtcatc atccccaaag acatgcctaa aatagtactg accatccaca
     8941 atgaagaccc agaaaccgaa agccctaatc gattttgcat aattgaaggt agccaattca
     9001 ttaacccaaa atagccggca atttggacag ttaccatgat actaaggcca catgtttgcc
     9061 aagcaagatg ttttgattca aacagtctgc taagtttggt tttcttttcg gcagattgta
     9121 cagatttgaa ttcctttggt tcagtcaagc cgcgacgaat gaacaagacc atcaacaccg
     9181 gcagtacacc tacgacatac aacatcttcc aaccaaacgt tggaatgatc acagcagcta
     9241 aaatcgcagc caaagccgca ccgacctgcg caccaatacc agcaaccgaa gaagctcgtc
     9301 caatttgttt ttttgaaaaa ctttcggaaa tcaaggacat acacgcccca tattctcctc
     9361 ccgctcctac acctgcgata aagcgaaaga gataaaccaa gtagatgttt gaagcaaaat
     9421 acatcaataa agaagcaatc gaaaacagaa ggaccgtttg agaaaatact ttcacacgtc
     9481 catatttatc agctatgatg ccaaaaaaaa tacctccaat gagcatcccc aaattagtga
     9541 tcgttgaaat gaaacccgct tgcgtaccac taatatgaaa agaagtaatc attgaagata
     9601 atgaaaaagc taaaaacata atatccatat tctctaaagc aattcccgcc gatgtcgata
     9661 ctaatacctt tcgctgatac gttgtcattt ttatacccct tttcctaaaa tttccctaaa
     9721 ttgttaaaag tatatccaaa aaagaataaa aaaacaagac ttttttattt gaaataatac
     9781 acaaacaacc gatatagata atccagctta gttttaatag aaatgagtat tgacttggag
     9841 tacacttaaa gttgtagaat aaataaaata cataagtaaa gtggcaagaa attaagagaa
     9901 agagtgatca gatgaatatc aaacaagtca gtgaagaaaa gggcatttcg gccgatacat
     9961 tgcgctatta tgaacgaatc gggttgattc caccagttaa tcggacaaaa ggcggaatcc
    10021 gtgattatac agaagaagac ctaaaatggg tcgactttac gatctgtatg cgaaatgccg
    10081 gattatcgat cgagtctttg attgaatata ttcgcctatc tagcgaagga gaagcaactg
    10141 tatttgagcg gcgtaagctt ttgattgaag aaagtgaact tttgacgaaa aaaattgctg
    10201 aaatgcaaga atgtttggaa cgtttacaag gaaaaatcaa gaaatatgat cgtgtgttat
    10261 ctagaaataa aatggaatgt atcaagtaaa aaaatcaagg agcatcgcta tggagttacg
    10321 cttactaaaa tatttttgga caattgcaga agaaggaaca atctctcaag ccgcagaagt
    10381 tctacatctt acacaaccaa ccttaagccg acaactgaag gagttagaag agtaactagg
    10441 aacagaactg tttgtacgcg aaaaccgcaa aatgatcttg acagaagcag gttattttct
    10501 aaaaaagccg agcagaagaa attttagatt taacgaatca aaccactcaa gaattcgaga
    10561 acagaaaaaa acaattattc agcggccgat ttacgatcgg ctgtgtggaa gctgatagtt
    10621 cgaatacatt ggcactatta ctagaaaagt tgatccgtga ttatcccagc gttcaatttc
    10681 atatccacag cggaacaagt gatgatatca cggagtgact agaaaaaagc ctgttagatt
    10741 tagcgatttt gctagatcca gtagatacag agaaatacga aacgattttg ttaccaagaa
    10801 aggaaaaatg gggattgctc attttagaaa attctgtttt agcaaaaaaa gacgtgataa
    10861 caaaagaaga ctttgcgaat gtacctcttt tgatctcaaa gcgtccagaa atacagcgaa
    10921 tgtttgctga ctggttaggc ataaaaaagg agcagttgct gatcacagga acgtacaatt
    10981 tgattttcaa tgtatttgcg ctaattgaga atcaggtagg tgctgcttta gtcatagaag
    11041 gtgctgtaag tcatcattca aagcgagtga cattcgttcc gcttgaccca atgattgaaa
    11101 cgaactgcat gctagtctgg aaaaagaatc ggattttatc acctgtagtc aatgaattga
    11161 ttcaccgttt caaagaagct tttgaacagt aataagtatc tctaatcgaa gaaatttcaa
    11221 tacaaaagcg agacgtatga cagtcttgct tttgtatttt ttcattttta aatcaaaaaa
    11281 ataaagagat ttacataact tgttaaataa gcgcttacaa aaataagaag aacggaaatg
    11341 aatcagctat aataaccgtt gttctggatt gtaatcatta caaagaaatg aagattataa
    11401 ctttggaggt gttcgaaatg aaagaaaaaa agcaaaaatt cgttcaaaaa acatgccctt
    11461 tattcaatgg ttgggtatga ttctgctgat tgtattagct ttattgacga tcattccttt
    11521 tttatggatg gtttctgctt cattcaaagc aaataatgaa gtgttcacga ttccgattca
    11581 atggatccct aaaacttggc atttagagaa ttatctggtt atttggtctc gtattccttt
    11641 agtgacattt tttaagaata cagctttttt aagcgttgtg atcacgttga ttcagttgtt
    11701 tacttctagt tttgctgctt acgggttttc taaactaaat tttaaagggc gcgatatttt
    11761 atttttagca tatatcggca cgattgctgt cccttggcag tcttatatga tcccgcagtt
    11821 cattatgatg agaaaagtgg gtttaacaga tacgttgtgg tcgttggtgc tattacaagc
    11881 gttcaatgct tttggtgtgt tcttgttgaa gcaatattac agtagtatcc cagattcttt
    11941 atgtgagagt gcgcggatcg atggcttatc agagtgggga atctatcgca agatcatttt
    12001 acctcttaca aaaccggcga ttgccagttt gacgatcatc acattcgtta atacatggaa
    12061 cgattatatg gggccattca tttatttgtc ttctacggaa aataaaacaa tccagttagg
    12121 gttgaaaatg tttattgggc tatttgatgc agagtacgca ttaatcatgg ctgcctcagt
    12181 tgtttctgtt cttcctgttg cttttatatt tttagcgatg caaaaatatt ttgtagaagg
    12241 gatcgcttca tcaggaatga aggaatagga ggtgattatg aaagagcagc gttttgacaa
    12301 aaatgtttat atggaattct tttattggtt gtatcaatca ttggcaagta gcctttgttt
    12361 ttggtgtgtc aatatcttgt atatagcagc tgcgttcctt ttgtcgatag atggaaggaa
    12421 tgtcttttcc tttatttttt ctttactttt tattggtcca ggttgcatcg tgatgatagg
    12481 tatgacagaa gaattagcaa aagaaaatag tttttcacca gtacgtttgt tcataaagat
    12541 gtttcgccaa ttttggctaa agggattttt ttattggcta tttgcttgga tagttagtgt
    12601 gataatgatt tttgattgtt tcttttttat acgtttttct tatggaaaat ggttgattcc
    12661 gttgtttgtt ttacttgctt gtttatcagt ttcctttagc accaactgct ggtattttca
    12721 ggtgagaaat ccagcgtcga aaccaaatca agttctacgg atagcgttct actatacgtt
    12781 gaaaaaatgg tatgtgagtt tactagattt tttactattg acaagtttgt ttctcttctt
    12841 tttcgtaaag ccccaatggt gtattctact aggtccttct atcgtatttg gtttgattta
    12901 cttcaacaat cgtaaattga tgagaactat ggatctgtag actttataaa ttgaaacttt
    12961 caattaagaa tgacagtttt ttttatgtaa aaactgtcat tctttttcat tttttgaaac
    13021 atatggctaa acaaatgaaa taaaacgtgc tatacttttg tatgaaacag gtttagaaac
    13081 tgtatcactt atttgtgatt atttctaact aataattaaa aataaagcat aaaaaggacg
    13141 atgttcaatg gctgaaaaaa tgaaacaagg ttcgaaatct gtagtagaaa gtttgattaa
    13201 tcatcatgtt gattatgtat tcggtatacc aggtgcaaaa atcgatggtg tatttaatga
    13261 attagaggat caaggtcctg aattaatcgt gactcgtcac gaacaaaatg ctgcatttat
    13321 ggcacaagca atcggacgta ttacaggaga gccaggagta gttatcgcaa caagtggtcc
    13381 tggtgctagt aacttagcta caggtttggt aacagcgaca gcagaaggtg acccagtttt
    13441 agcaattgcc ggacaagtaa aacgaagcga tctattgaag ttgacccatc aaagcatgga
    13501 taatgcagca ctgttccagc caatcacaaa atacagtgca gaaatccaag atccagaaac
    13561 aatttcagaa gtgattgcca atgcttatcg tatggctaag agttcaaaaa aaggcgcgag
    13621 ctttatcagt atcccgcaag atgtagtgga tgcacctgtc caaggtaatg tcatcaagcc
    13681 attgagcgat cctaaattag gttctgcttc tgcagatgat atccgttacc tagctgaacg
    13741 cattagagaa gcaaaactac cggtactatt agtaggtatg cgaggatcaa gtgaaaaaga
    13801 aacgttagcc atcaggcaat tagttgaaaa gacagcattg cctgttgtag agacattcca
    13861 agcagctgga gtgatctcac gtgaattaga agcgcatttc tttggtcgtg tgggtttatt
    13921 tagaaaccaa ccaggagata tgcttttgaa acgaagtgat ctagttattg caattggcta
    13981 tgatccaatc gaatatgaag caagaaactg gaatgcggaa aaagatgcgc ggatcattgt
    14041 gatcgatgaa gcgccagctg aaatcgatcc ttttatgcag cctgaaagag aattgatcgg
    14101 tgatatctca gccactcttg atttattgac aggttcatta gagcctcaac aagtgtcaga
    14161 agatgcaaaa gaatatttag catctcttca agcaaaatta acggagcggg acattgttca
    14221 atcaaaagga gaagcaggta tcttgcatcc tttagaagtt attaacacgc ttcagtcaaa
    14281 agtaacagat gatatgactg ttaccgtaga tgtaggtagt cattatattt ggatggcacg
    14341 ccactttaga agttatgaac ctcgtcattt gttattcagt aatggtatgc aaacgcttgg
    14401 tgttgcattg ccatgggcga tttctgctgc attggtgcgt ccaaatacac aaatcgtatc
    14461 agtttccggc gatgggggat ttttattctc tgcccaggat ttagaaacgg cggtacgtaa
    14521 aaaattgaat atcgttcatt tgatttggaa tgatggacac tacaatatgg tagagttcca
    14581 agaaaaaatg aaatatcagc gggcgtcagg tgtagacttt ggtccagttg attttgtgaa
    14641 atatgcagaa gcttttggcg ctaaaggaat ccgagctaca agtgtagagg aattagaaaa
    14701 agctttagaa gaaggatttg cgacagaagg acccgtaatt attgatattc cgattgatta
    14761 ccgtgataat gagaaattgg gagaaacaat cttaccagat caattctatt aaggaagtgt
    14821 cgacatgact gaaaaaatct tataccagca cgggacatta ggagctttga tggccggctt
    14881 gatggatgga acagaaacga ttgctcatat attagaaaaa ggaactctgg gattgggaac
    14941 attgcatggg cttgatggtg aagtcatctt tttagatggc gtagcttatc aaggaagatc
    15001 agatggctca gttgcccaat tagatggaag tgaacttacc ccctatgcag caattacaga
    15061 ttttacgcca gatactagtt tttctgtctc aaaaactgca gatggagaac aattgaaaaa
    15121 agacatatta gtaagagaag ccggggaaaa cctatttttg gcagtgaaga taactggatt
    15181 attcaaaaat atgcacatcc gtatcatgcc gaagcaagaa aaaccttaca gaagactagc
    15241 aaaaatttca gagtctcaac cagaattcca acaaagcaat atccaaggaa cattagtcgg
    15301 gttctttacg cctgaattat tccaaggagt tgctgcagcg ggatttcacc tgcattttat
    15361 cgatgataca catacatttg gcggacatgt aatggatttt gaagtggctg aaggaaaagt
    15421 tgagattagc cgcatctcgt cccttgtaca gcattttcca gttgatgatc cgacttttgt
    15481 tcacgaaacg attgattatg ctgatttagc agcagaaatc caacaagcag aatagaaaaa
    15541 gacagcgaga tgcgttcaag gtgattaacc tgacgtatct cgtttttttg aggaggaaac
    15601 ctagcgttta caaaaaaact gagctataat cttctccata gatcaaaaag gagcggtttt
    15661 tatcatgtgg acagaaaaac agaaaaaaga tgcatacgaa attttattat tacagcaaca
    15721 aggattatta gaagcagaag ataactggtt agcaaattta gcaaattcat ctgcattgtt
    15781 gaatgagact ttgcctgaga ctgtctttgc aggatattat ttattcgatg gtaatgaatt
    15841 gattttagga ccgttccaag ggcgagtttc ttgcacacgt atcaagatgg gaaaaggagt
    15901 ttgtggagaa tcagctgaaa aacaagaaac attgatcgtt gatgatgtca aaacccatgc
    15961 aaattacatt tcctgcgatt cagccgcaca atcagaaatc gtagtgccaa tggtaaaaga
    16021 aggtaattta atcggtgttt tagatatcga ttgcggagta acgaaagggt atgatgaaat
    16081 cgatcaagca tttctagaaa aatatgtaga tcttttatta gcaaatttta atataaatgc
    16141 tttttcattt attaataact aaaatcgtct attgaaacaa aaaagaagag aaatcaagaa
    16201 taaaaagagc cggaaagaca gtggtcattt ctgtttttcg gtttttttat ttttttataa
    16261 aatcacaagt gttattgatt ttcacaatca tattttaatg gtattatcat tttgaataat
    16321 gaaaaaacat taaggatgga tgaaatatga ataacgaaac tatcatttta ggaaaaaaat
    16381 atatttgcca acctattggc ttgaaacaaa aagtggttgg agaagtaatc agtaaattag
    16441 aaaattgtgt tgtgatctgc gtagacgaat atcatgacgt cgatcgcaat gaaatattag
    16501 aaaaatgcgg aaaagtagtg gcaaaatata ctcacgttta tgagttggaa acaacagaat
    16561 atctatataa ctatagcaac aaatatgaac atactttagt ttcataaaga tttaaataaa
    16621 gtgccttcaa aagcgagaag accaagcttt tgaaggcact ttatttagtt ggcagtgatt
    16681 gttcatgatt tttgatttat tcaaaaggtt cgcctagaga atctgtctct gtaacgaggc
    16741 ctggtggaga aactcgataa aaacctatac tgccagaact gtcctatttt cggatagaca
    16801 aactagtagt tttaaaaaga tcatccgatc caatcatttc aaaaaatgca aaactgatat
    16861 caggatcatt tagctcaggc ttgttatcgt aaagcatttg taaaggttgc atttagttag
    16921 gagtaaacgc gaaactatca tcattatttg ctgaagaaga tgaagaagct aattcagagt
    16981 ttgtttgaaa taaaggagac gtgctgacag tttctttacc ctgttttttg cagaggtaga
    17041 aacttttata tcggaagttt ctaccggaga atttttctca tttgtagaaa ttgttgaatc
    17101 gatactattc gaaatttgcg tatctgaagg tatgttcttt gatgcagaag tcccacaagc
    17161 agtaagtaac aataaatttg ataaactgat aaacagaatt cttttcatta tgtacacact
    17221 ccttatagta tcgtttagca tacacctatg ctcagtatta ggcaaaatca atgcaaaact
    17281 atcaagtctt ttatagtatc tattagtctt agtaatacat atatggagag ggtgatatcc
    17341 cagttcaaac cagatacatt ggttaccatc tgtattaacg attatgcctc taaacgcttt
    17401 ttttatgaaa aaatttataa gtgaaaacgt aggtatgata ttatctagag gtaatttttt
    17461 tgggtattta aatagttgct aggaggatag cttttggatt tttacagtaa ttttatctta
    17521 ataatagcaa tattattact tctaaatatt tggttttttg ataagagtag aaatgcaggg
    17581 ataggtttta gaaccaaacg aagtacgtct tctgaaaaaa agtgggtata ttcacagact
    17641 attttttacg gaggggttat ttcaatcagt ttactttctt caacacttta ttcttttaat
    17701 gttatagacg tttccatgtc aaattttata tctattattg gaatccttat ttctgcaatt
    17761 attacacaac tacttcttgt gtttgaagag aagtcaaaaa ataattaaag caattatgcc
    17821 ttttagtaca ggaattatta cttcttacaa atgtatagcg aatgtgaagc ctgctgtttt
    17881 aattgttcta gaaaaacttt tgagtgaaca attattttat gttaggatga attaatttct
    17941 ctagagttta taattaaaaa acttatttga aggaacatgc ttaaaccata attgtgtttt
    18001 tccttccatc agcataaaag ttcacctcaa aaagagtgta cgaacaaaag ttctttttgt
    18061 aaaccttaaa atagattttc ctgaatacgt attggggatt tctaaatgtt tgaatgtttt
    18121 tttgaatgca aaatcaatgc aaaaaatttt gtatttctct acttttctct ccttgaccaa
    18181 aataaaaaaa cgttgattta acaacgtttt tcatacatgt ttatctgtct caaccataca
    18241 tatatggaga cggcgggagt cgaacccgcg tccaaacaca tcgccactta aaaatctacg
    18301 ttcatagtca actcatttaa ggattcactt tacagcttgc cgagtgacag gcattctgta
    18361 tcgctagtct gatgttctct ttttcaactt acagacggaa agttgaaacg tatcccacta
    18421 aagttaggac ccttactcga gcacatgggc gatgccgaga ggatctacgc taactgtttt
    18481 taggcagcta aagcgtaaga gttgttttcg tttttagcag ttatatttaa ctgtaacgtt
    18541 tttacgtaga cgtaacctac gaaacgcaat tcaagctcga ctgtgcctgt cgaatccgta
    18601 acgtccccgt tagctaacaa gggttacgtg tatttttcat acaaaattag tatagcatat
    18661 attgggataa attcaataaa aaagcgaatc atatcccatt atttatataa taactgcccc
    18721 tttcttaaga aaaaataagc gattttctta agaaaagagg tagcttcaaa aaaagatctt
    18781 aaatcagatc aaaatacttc aatgcgtgga caattccgcc ttctgtattt ttctttgtga
    18841 taaaggtaga aagttctttt aatccgtcat aggcgttgcc catagcgatt ttgttgtcgc
    18901 aagcttccaa caaggcaaaa tcattcggac catcgccaaa tccaaaagta ggaacttttt
    18961 ccagctgaag ttctttttgc agacgtttga cacctgcccc ttttgagatt cctttcttca
    19021 ctgtatcgat cgagtaagga ccattccgat aaaaagttag ctcaggaaaa tgttcgtgat
    19081 agtattcgtc acctgattga gataagatca gcaacatatt tactgtgttt tctttaaagc
    19141 ctaaaggatc aatttccgga acatctgaat gaatgaagtc gtaagcattg atcattgttt
    19201 ggctatgacc ggtacaccag attttctgat cgttatagta agaaagttca tgaccgttca
    19261 actgtacatg atcatacatt ttttgacatt cttctgaaga aaatgtttga gaatagattt
    19321 cttcgccgtc tactcgaatg aaagaaccat tcatggtgac cgcagaagta atgcccgcat
    19381 cttccatgat agagtggatt tctgcttctg tacgtcctgt agcgatgacg gggagaacat
    19441 tgttttcttt taatgcgttc atagcttttg ccacttcggg tgtgattttt gatttcgcat
    19501 ctagtaatgt tccatcaaga tcaaagaaag tgattgcacg ataatttgac acaaataaaa
    19561 ctcctttttt attggaataa gtttatctta acaaagaatg aacaaaagac aaacctttcg
    19621 tgaagaattc ctgaacatat tggttcagtc aacaatttag agtatactat aagaagagaa
    19681 tttaggaggg aaagaatgat ttttttcagt attggtatct tattcttaat aattggcagt
    19741 ctttttcgga ttctgccctc aaaaggaaat ctgccttttt atgggtatca ttcgcctctt
    19801 gccgcaaaaa cagacgctca ttggcggctg gctcaaaaga caagcggaaa ctggtttttt
    19861 cttatgggct tactgatggc attgatcggc tattatttaa agacatctgg gcatacgaat
    19921 tattttctga ttgaaatgct tctgctagta tttccaatta tgcctatttt tattatgaca
    19981 gaaaagaaat tacagaaata tgacctggaa actggaggga acgataatga atattttaat
    20041 gattgaagat aatcaatctg tttctgaaat gatgcagatg tttttcttaa atgaaggttg
    20101 ggaagcaact ttcaagtatg atggaaaaga agggctcgat gcctttttag aaaacccaca
    20161 acattgggat atgattacac tagacctgaa tttgcccaca ctggacggta tgctagtagg
    20221 aagagagatc cgcaaagttt caagtactgt gccgattatc atgctgacag ctagagattc
    20281 tgaaagcgat caggtgatcg gcttagagat gggggcagat gattatgtca caaaaccttt
    20341 cagtcctctg acgcttattg ctcgaatgaa agctttgcat cgccgtagtg agttggcaga
    20401 agcaagagaa gaagcagcag tttcagatga tcattttgat atcgagacta gtcatttcaa
    20461 gatgaatacg aaaacacgag aagcgtatct ggacaaccgg ccaattgaag gactgacgcc
    20521 taaagagttc gatctattgt ttactttagc aaaaaaacca cgtcaagtat tctctcggga
    20581 gcagttgctt gaattagtct gggattatca gtactttggt gatgaacgga cagtggacgc
    20641 ccatatcaag aaattacgac aaaaaatcga aaaggtcgga ccacaagtca tccaaactgt
    20701 ttggggagtc ggctacaaat tcgatgattc tggtgttgcc taatgcgtta tttgtatcag
    20761 caactcttag ctttttgcgg aatggtcgcg ttgatcttat tgatcgttgg agtttctttt
    20821 acacaactga caaaacaaac gattgaagaa aacaattacc agcaactgtt tggatatgct
    20881 gaatcagttg aaaagacaac gcaaacctat gccgatacgc tcccgcagtt tagtcaagat
    20941 caggcgtttc aaaatgcatt gctgctgaca gagcaagtac tgacagaaca aaatgtgaac
    21001 tttatattta tcgataaaaa cgaacgtgtc atttatccga ctaatacagc tttactgaat
    21061 tttaccttaa cagctgatca atggaaaaat ctgaggaatg gacgtcaaga aaaattcaca
    21121 tcgaataaaa atattttagg acaagcacag gcgacctctt acgcacttgt gccatttaat
    21181 ttgaatcatg aattctatgg agcacttgta gttagtcaac ctgcgcgaaa tatcgataac
    21241 agcgttcgtc caattacact gaacctgttc aaaggattta ttttttccag tatcgttgca
    21301 gttattgcca gctatgcttt tgcagcattc caagttaagc gtatcaatcg gttaaggaat
    21361 gcgacgaaag aagtgacgaa tggtaatttt gatgtacagc tccccgtaca tgacaaagat
    21421 gaatttgatg agttagcaga tgatttcaat aaaatgacga attcattgaa agaatctcaa
    21481 gcagaaattg aagaacaaga aaatcgtcga agacaattta tggcagatgc ttctcacgag
    21541 atgagaacac ctcttacgac aatcaacgga cttctggaag ggttagaata caatgcaatc
    21601 cctgaaaatc agcgagaaaa cgcgattaaa ttgatgaaaa atgaaacaga gcgtctgatc
    21661 cgtttggtga atgaaaactt ggattatgaa aaaatccgta cgaatcaaat cagtatggtc
    21721 attaaaaaat tcaatggaac agaaacattg aaaaatattg ttgcacagtt agaagctaaa
    21781 gcagaagcag caggagatac attgacattg aaagccgttg atgatattga tgtttacgct
    21841 gattatgacc gatttgtcca aatcatggtc aatattattc aaaacgcgat acagtttact
    21901 gaaaacggcc aaatcacgat tactttagaa aaaggctatc ttgaaacgat catcacgatt
    21961 gaagatacgg gaattggaat gtctgaacaa caaatgaaaa gtatctggga tcgttattat
    22021 aaagtagatc cttcaagaaa aaatacaaaa tatggtgaat caggattagg tttaccaatc
    22081 gtgcagcaac ttgtacgctt gcataaagga aaattagaag tagaaagcga actaggaaaa
    22141 ggaacaaaat tcgctgttcg tttgcctgac gtagaaatca aaaaagagta aaaaacaagc
    22201 ggtataaaag atcatgaatc gatcttctat accgcttgtt tttactataa aatgattgat
    22261 ttagcccgcg ggctctaaac gatcaatata aggactttga gcaatagtag catcatgagt
    22321 aaagagtaag ataccttgat tttcttcatg agctaaataa gaaagcaagt ggagtagctc
    22381 ttggatctcc aatacactaa gttcatcaaa tgtatcttca ataacaatat tgtttcgctt
    22441 ggaaagcaag gcatgaagta attgcagctt tactttttca aaagacgtca actcagctgc
    22501 cgcacaatta agcaaggaag gagagatatc aagtttttct tgataggcca aacgatcttg
    22561 tttgatttct ttttccggta catccaacag gacatggtct ttaagattca gataaggaaa
    22621 catcggccaa tttttttgga tgatcgcaaa attatccagt tttgcaacaa gaacctcttt
    22681 tttcaagtgc tccaatattt tatgctgttt ttcgagagga cacaaaataa cggtcaagtg
    22741 ttcagcatga atcaaatatt caggtttcat tacgtccagg cctcctactt tgcaaatgat
    22801 ggataaaaaa agtgaaaggg aatagactaa gaatgagaca gcatccactg agcccaataa
    22861 gagtttgggc aaatccctta agtatatctg tgaaaaaaag atcattttgg aaatcgatgg
    22921 taaaaaaagt ctgttgatcc atcggaattg taagtccgct taccgaagtc aagatatcac
    22981 ggatatctgg cagttcagta gtctttaaga caagaaaatt gatcctttga agaagcgctt
    23041 cataaactga ctggaagctt acgatccaca ataaaaccaa gatacaacaa agaaagagag
    23101 gaaagaacag ctgccagaaa ataaaaagga ataatttggt tttagaaaga ccagtcaggc
    23161 gccaattgat caattcctgc ttgttttgcc tgactgaata gtaaaagaaa aagataaaaa
    23221 ggaaggcaaa tagaaaaaat aaactgaata tgccttttat ataaaatcgg ctagctaatt
    23281 ctaaagccgg aagaagctct gtagatgcag aagcgccaat aaatgaaagc cgatcttgga
    23341 cttgattggc taaagccgat tgcaaatggt aaagagtcag gcatgctatc aataaaaaag
    23401 cagaaaagct tgaaaataaa caatagagta ttgtctgctt tttgtaataa attaatcgag
    23461 cagtagcata gcggattatt ttcatacttt tcacctctat aaaaagtata gaaaatgaat
    23521 atgaataaag tatgaaattc ctttagtgaa atcacgaatt aaaaccagaa aatattttgt
    23581 aaaaagcaag aggttgtgaa gaagctgccc tattctatta ccgtagatgc aagcttttga
    23641 acactgttta ttcgaaaaga ggagcagcga caataatttt gtcgctgctc cttttaaatt
    23701 tattttgtca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac gtttgcgttt
    23761 tataaagatc aacagttgat tgtccgttgt gttcactaaa gacactagtt gacttgtcac
    23821 caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca taatcactgg
    23881 tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca ttattgattt
    23941 ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt tcattctgta
    24001 cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc gtaccaccgt
    24061 agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc tgttcatgat
    24121 ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca cctagtaagt
    24181 gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt ccgccctgtg
    24241 tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg tcgtagtttg
    24301 tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt gaattcgaga
    24361 caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg ctttctttca
    24421 aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca aagtaaccgt
    24481 tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg gtgaattttg
    24541 aataaggata atggttagat acagcaatga attttgaata aaatggttgt tgcagacgtt
    24601 ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat tggaacgaat
    24661 tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct aaattcttgt
    24721 atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca ctagtgtaac
    24781 cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg cctaattgtg
    24841 tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt tccgcatcgc
    24901 ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg ctgttgctgt
    24961 ggtaaagact attgatgaaa ggtgtaactt catgctggag accattctca tctgtcaatt
    25021 tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca ttttttcctt
    25081 tagcgagccc gaacaaatcg tcatttggtg ctgcataatg ttcttttaca taattttcta
    25141 cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg taagtttgaa
    25201 caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa tcacgagaga
    25261 atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt cctgagaaaa
    25321 tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg cgagggtcca
    25381 acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc caatagatca
    25441 catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct actttcccag
    25501 cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa gtgacattcg
    25561 aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa gcaactctag
    25621 ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata gggttgataa
    25681 acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact aaataggcaa
    25741 aaatcgtttt caaccaaagt aaaacgacta gtaaagaaaa aaatccgagc ctcttattca
    25801 aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt cttaatcaca
    25861 gaagtgtcat gaaaccatgt tacacagctc acttattctc atttatttta ctagtatgac
    25921 aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt ctttaagctt
    25981 catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg gaagagaaag
    26041 ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat cgattgaaaa
    26101 aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa accacgcaat
    26161 gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta ggaaagcaga
    26221 ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa tctttttttg
    26281 aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat gattctacta
    26341 ccgcttttcg tatattcaat ggagaaggag acggaatcgg cggtctgatt gttgattatt
    26401 atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat cgtgaattat
    26461 tgattcaagc ttttcgtgaa atctacccgg aaatcgtagg aggctatgag aaaattcgat
    26521 ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca ccggaaccct
    26581 tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga ttaatgactg
    26641 ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc gcttctggga
    26701 aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc gcaatgggag
    26761 gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa acacaggaac
    26821 agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg gacgtattcg
    26881 agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta ttggatccac
    26941 caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat ggggaactgg
    27001 tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct acaaatgctg
    27061 cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat gccaaaaatg
    27121 tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg acggatacct
    27181 ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac cattagaagg
    27241 agcgatgcag aatggtttgg gtaaagggat tgaatatagg aagcggtaga ccaaagattt
    27301 gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt cttgcacgtg
    27361 aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt ttttcttttg
    27421 atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg ccgttattat
    27481 ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt cagtatcgcg
    27541 acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc gagttgttca
    27601 aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac attcctttaa
    27661 ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt taccgattga
    27721 atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc aataatgaac
    27781 gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc tccatgccta
    27841 ttattacgat gtccatggga aatcttggta aaatttctcg tctggctggc ggaacgacgg
    27901 gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca ggacaattac
    27961 cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag aacaagtttg
    28021 aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg tatggtcgaa
    28081 caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca cctaagtgcg
    28141 tcatcggtcg ctgaacagct tccagaaagc caatctcgta tatttaaaac actggttgct
    28201 gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga attgaatcta
    28261 aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt aaaagatttg
    28321 gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa aaaactattt
    28381 cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc ggctggcaga
    28441 agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag cggacaattt
    28501 gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg atgttgtttg
    28561 tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc attcctgcta
    28621 catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca ttgacatcca
    28681 gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt ttaggataaa
    28741 tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga aaagttttgt
    28801 tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt ttttgagagg
    28861 cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact aacaatcgct
    28921 caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta cttttttctg
    28981 tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga gaaagtgtca
    29041 ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc agtgcagccg
    29101 gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc aatacacgac
    29161 cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga agtgttttct
    29221 cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga ggcgtcaccg
    29281 tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta gatggataag
    29341 tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat tccgtgcgta
    29401 taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag aatagaagcg
    29461 tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt gatttatttg
    29521 ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt gattcaaaga
    29581 agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac tttccatttc
    29641 caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg caccgaccac
    29701 cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag agtacgcgtg
    29761 cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg ccgactacga
    29821 tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata tctgaagaaa
    29881 gaaaacagaa aaaaaatgtt ttgagccaaa agagaaaatg agctaaaatg agggaagata
    29941 agcaaaggag cgtggaacga tgacaaacgc aacagaatat gttcaagcaa ttcaagaaaa
    30001 gctacatcaa aaggatcaag ggcaagaaga atttttacaa gcaatcgatg aattcatgcc
    30061 cacggttatg tcttttttgg acactcatcc cgaatatatc gaaaagaata tcttagaact
    30121 tttaacggaa ccagaaagag ttattcaatt tcgagtgcct tggcaagatg atgaaggaaa
    30181 ctggcgagta aatagagggt accgtatcca atacaactca gctatcggcc cttataaagg
    30241 tggattgcgc ttccatccta gtgtcaatct aagtatcttg aaatttttgg cttttgaaca
    30301 gattttcaag aatagtttaa ccggtctgcc aatcggtgga ggaaaaggcg gaagtgactt
    30361 cgatcctaaa ggaaaatctg ataatgagat catgcgtttt tgtcaaagtt tcatgctgga
    30421 attgtcaaaa catatcggcc catcgattga tgttcctgcg ggagatatcg gtgtaggagc
    30481 tagagaaatc ggctatttgt tcggcgaata taaacgattg aaaaaatatg atactggtgt
    30541 gttgacaggt aaacctttgg atttttgggg aagtaagata cgtacggaag ctactggtta
    30601 tggattagtg tattatgtca aacatctatt gaacgaagaa aaagattcat ttaatcaaaa
    30661 aacagttttt gtttccggaa gcgggaatgt agccatttat gcaatcgaaa aagtgcaaga
    30721 gctaggcggg aaagttgtta catgttcaga ttctagcggg tacatctatg atccagaagg
    30781 aatagatgtg aaactattga aagaagtcaa agaaatcaat agacaacgtt tgacggaata
    30841 cgcaaaccaa cgtccttctg ctatttatca tgctggagaa tctgtttgga cattgaaaga
    30901 aaaagcggat atcgctttgc catgtgctac tcaaaatgaa atagacgaaa gcttggccga
    30961 aatcttagta gaaaatggga tcaaaatcgt agcagaaggc gctaatatgc catgtacagt
    31021 aaaagctgtt tctgttcttc atgaagcagg cgtgtggtat tgtccaggaa aagctgctaa
    31081 tgctggagga gtagcagtat ctgctttaga aatgagtcag aacgtccagc gattgacttg
    31141 ggaaagagag caagtggatg agcagctgga tcaaatcatg gaaacgattt accatacgtg
    31201 tcgccaaaca gcagccgagt acggcgacga aaaaaatctg cttcttggag caaatgttgc
    31261 tggatttgaa aaagttgcca aagcaatggc agcagaaggt ttagtttgaa aaatgaaact
    31321 ttcttggaaa tattgtcagc attttgttta tttgcttgaa aaataataaa aaaaaacgta
    31381 aagtattacc tgtagagtaa acctataagg aggagaacaa atggcacaca ttcattttga
    31441 ctattcaaaa gtagcaccgt ttgtcaacga acaagagtta gggtacatgc aaagccaagt
    31501 aacagcagcc cacaacgaat tacgggaagg tactggcgca ggaagcgact tccgcggctg
    31561 gatcgattta ccaacgaact atgacaaaga agaatttgca cgtatcaaag aagcagctaa
    31621 aaaaatccaa tctgattctg aagtattagt tgttatcggt atcggtggtt cttatttagg
    31681 cgctcgtgca gcgatcgact tcttgaatca cacattcttc aatttattag atagcaacac
    31741 aagaaaagca ccacaaatct tctttgccgg aaattcaatc agctcaacat atattgctga
    31801 cttgatcgaa gtaatcggtg atcgcgactt ttcagtaaac gttatttcaa aatctggaac
    31861 aactactgaa ccagcgattg cgttccgtgt attcaaagag cttcttgtta agaaatatgg
    31921 acaagaagaa gcaaacaaac gtatctacgc tacaacagat aaagcaaaag gtgcagttaa
    31981 agtagaagca gatgcagaag gttgggaaac tttcgtgatt cctgatgaca tcggcggacg
    32041 tttctctgta ttaactgctg ttggtttgtt accaatcgct gttagtggtg cagatatcga
    32101 tgcattgatg caaggtgccg ctgatgcaag caaagcatac tcaagcgaca aactagaaga
    32161 aaacgaagct taccaatacg cagcaatgcg caacattctt taccgtaaag gcaaagtaac
    32221 tgagttgttg atcaactatg aaccaggaat gcaatacttc tcagaatggt ggaaacaatt
    32281 gtatggtgaa tctgaaggaa aagaccaaaa aggcatctat ccttcaagcg caaacttctc
    32341 aactgatttg cactctttag gacaattcat ccaagaagga cgtcgcaata tttttgaaac
    32401 agttgtgaag gttgaaaaac ctcgtaaatc aatcacaatt cctgaacaag cagaagattt
    32461 agacggtcta ggctacttgc aaggaaaaga agtggacttc gtaaatacaa aagcatttga
    32521 aggaacattg cttgctcata cagacggtga cgtgcctaac ttgctagtca aaattccaga
    32581 aatggatgct tatacattgg gttatacaat gtacttcttt gaaattgctg taggtatttc
    32641 aggttacttg aatggaatca acccattcga ccaaccaggt gtagaagctt acaagaaaaa
    32701 catgttcgca cttctaggca aaccaggctt tgaagatctt gctaaagaat tgaacgaacg
    32761 cctgtaagat aagggttttt cgctagattt aaataatcag tgatagaaac atgttcgctt
    32821 taaaatgaaa actggtaccc atttaggtac tcaaacatac tgcatcgttt attcagaata
    32881 aggggctgtg acaagacgtt tgtcacagtc ccttgtttgt atagaaatcg attgattgcc
    32941 ttgctcttta gtttgtttta ggactttaag aacagtgagg ctgacttcga aggttaattc
    33001 tgtttttttg ttttctagaa tcagtaagca aaaaagcact cacttcgagt gcttttttgc
    33061 gatatattct tagcttaaga taggcctaat ttttctttta cagatgaggg taccttatcg
    33121 ctggtaatta cttttttctc tatgatccgc ttttcgcttg ctttcaactg caagatcgtt
    33181 ccttcggggt aagcagaacc attggcagag tcaccagaag taatgacatc gaaatctaac
    33241 tgtctttttt caccgttttc agcataagca tctacgttgt atttaaaacc tcttccaatt
    33301 acttccccat tatctgcttt gatatcagtt tctgcaggca ttggagcttt gactaccgca
    33361 taataatctt tacctacata agtagactga tagtagtccc aacctttcca tcctataaaa
    33421 gcaatcaata tgataacgat caaccctaaa atttttttca tgaaagatct ccttttttat
    33481 ttttattata cccgtggtgc tagttaattt taatatccta aagaatatct caaagttcct
    33541 tcaaaacgtc gttgtgctcg acttaataga acactgtaag ccagtaatat gctttcaaat
    33601 ctactctcta gccctttaac agaacgtgag ttgaaatttt gacaacctaa tttttctaag
    33661 gaagaaaaaa cagtctcaac tg
//
LOCUS       DAYYFU010000026        33621 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000026 DAYYFU010000000
VERSION     DAYYFU010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.026"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            103..1764
                     /locus_tag="ACHA34_001448"
     CDS             103..1764
                     /locus_tag="ACHA34_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8792095.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1834..2181)
                     /locus_tag="ACHA34_001449"
     CDS             complement(1834..2181)
                     /locus_tag="ACHA34_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFM8792096.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2265..3206
                     /gene="opp3b"
                     /locus_tag="ACHA34_001450"
     CDS             2265..3206
                     /gene="opp3b"
                     /locus_tag="ACHA34_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8792097.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3207..4250
                     /locus_tag="ACHA34_001451"
     CDS             3207..4250
                     /locus_tag="ACHA34_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8792098.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4256..5326
                     /locus_tag="ACHA34_001452"
     CDS             4256..5326
                     /locus_tag="ACHA34_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8792099.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5316..6269
                     /locus_tag="ACHA34_001453"
     CDS             5316..6269
                     /locus_tag="ACHA34_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8792100.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6281..7291
                     /locus_tag="ACHA34_001454"
     CDS             6281..7291
                     /locus_tag="ACHA34_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8792101.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKRMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7576..8397)
                     /locus_tag="ACHA34_001455"
     CDS             complement(7576..8397)
                     /locus_tag="ACHA34_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8792102.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8494..8772)
                     /locus_tag="ACHA34_001456"
     CDS             complement(8494..8772)
                     /locus_tag="ACHA34_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792103.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9040..11049
                     /gene="metG"
                     /locus_tag="ACHA34_001457"
     CDS             9040..11049
                     /gene="metG"
                     /locus_tag="ACHA34_001457"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFM8792104.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11528..12115)
                     /locus_tag="ACHA34_001458"
     CDS             complement(11528..12115)
                     /locus_tag="ACHA34_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792105.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12290..13612
                     /locus_tag="ACHA34_001459"
     CDS             12290..13612
                     /locus_tag="ACHA34_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8792106.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13767..14543
                     /locus_tag="ACHA34_001460"
     CDS             13767..14543
                     /locus_tag="ACHA34_001460"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8792107.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14536..15105
                     /gene="rnmV"
                     /locus_tag="ACHA34_001461"
     CDS             14536..15105
                     /gene="rnmV"
                     /locus_tag="ACHA34_001461"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFM8792108.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15098..15982
                     /gene="rsmA"
                     /locus_tag="ACHA34_001462"
     CDS             15098..15982
                     /gene="rsmA"
                     /locus_tag="ACHA34_001462"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFM8792109.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16433..17062
                     /locus_tag="ACHA34_001463"
     CDS             16433..17062
                     /locus_tag="ACHA34_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792110.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17186..18295)
                     /locus_tag="ACHA34_001464"
     CDS             complement(17186..18295)
                     /locus_tag="ACHA34_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8792111.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18504..18926
                     /gene="mgsA"
                     /locus_tag="ACHA34_001465"
     CDS             18504..18926
                     /gene="mgsA"
                     /locus_tag="ACHA34_001465"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFM8792112.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19113..19211
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19249..19785
                     /locus_tag="ACHA34_001466"
     CDS             19249..19785
                     /locus_tag="ACHA34_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8792113.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19825..21588)
                     /locus_tag="ACHA34_001467"
     CDS             complement(19825..21588)
                     /locus_tag="ACHA34_001467"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8792114.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21591..23306)
                     /locus_tag="ACHA34_001468"
     CDS             complement(21591..23306)
                     /locus_tag="ACHA34_001468"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8792115.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24085..24309)
                     /locus_tag="ACHA34_001469"
     CDS             complement(24085..24309)
                     /locus_tag="ACHA34_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792116.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24500..27277
                     /locus_tag="ACHA34_001470"
     CDS             24500..27277
                     /locus_tag="ACHA34_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8792117.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            27358..27777
                     /locus_tag="ACHA34_001471"
     CDS             27358..27777
                     /locus_tag="ACHA34_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8792118.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27789..28259
                     /locus_tag="ACHA34_001472"
     CDS             27789..28259
                     /locus_tag="ACHA34_001472"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8792119.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28276..29055
                     /locus_tag="ACHA34_001473"
     CDS             28276..29055
                     /locus_tag="ACHA34_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8792120.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29042..29863
                     /locus_tag="ACHA34_001474"
     CDS             29042..29863
                     /locus_tag="ACHA34_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8792121.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            29880..30929
                     /locus_tag="ACHA34_001475"
     CDS             29880..30929
                     /locus_tag="ACHA34_001475"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8792122.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            30942..31946
                     /locus_tag="ACHA34_001476"
     CDS             30942..31946
                     /locus_tag="ACHA34_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8792123.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32579..33598
                     /locus_tag="ACHA34_001477"
     CDS             32579..33598
                     /locus_tag="ACHA34_001477"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8792124.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        10973 a         5964 c         6882 g         9802 t
ORIGIN      
        1 gacaaaaaaa agtgttgatt ttttaataat tattcattaa actcttaaac aacttcagaa
       61 aatattaatt attctgaaaa ttccaataaa ggggtacgta aaatgaaaaa gaaatttaca
      121 tttggtttgg ttgcggtatg cggccttgta cttgctggat gttatggcgg cagttcagcc
      181 acaagcagta gttcagcaaa aagcggttca gcagatggag gaggcgtatt taacttagtc
      241 gtgcctcaag aaatgcctac agctgactta tcagttgcaa cagatacaat cagttttaca
      301 gcattgaata acgtttatga aggaatttac cgtcttgatg aagacagcaa accacagccg
      361 gcaggagcta gcgagttggc agaagtcagt gaagacggat taacttacaa aatcaaatta
      421 cgagaagacg caaaatggtc aaatggtgat cctgttacag cagcggatta cgtttatggc
      481 tggcaacgaa cagtagaccc agctacagct tctgaatatg cttacctgta tgcgccagtt
      541 gaaaatgcag aagacattac agctggaaag aaagataaat cagaactagg gatcaaagca
      601 gttggtgact atgaattaga aatcaaattg acgaaacaaa caccatattt ccaatatctg
      661 cttgctttcc catcattttt cccacaaaaa caatccgtag tggaagaaaa tggggatgcc
      721 tatgcatctg caagtgataa agctgtttat aatggaccat tcacattaga agattttgat
      781 ggaccaggaa cagatacaga atggacttat aagaaaaatg atcattactg ggacaaagat
      841 acagtaaaac tttctgaaat caaagtcagt gtagtaaaag aatcttctac tgcattgaac
      901 ttattcaaag atggccaagc agatgacgtg atcttgtctg gtgagttagc acagcaaaac
      961 gcaaacgatc cagcttatac ttctgttaaa gaagcgcgga caagctatat cgaattcaat
     1021 caacgtgaaa agaattcacc attcaacaat gtgaacctaa gaaaagctat ttcttattct
     1081 atcgatcgtg aagctttagt aaaacaagta ctaggtgacg gatcagttgt ctctacagga
     1141 ttgatcccat cagatatggt aaaaagtcca acaacgaacg aagactttgc aaaagaagct
     1201 ggaaaattag tttcttatga caaagataaa gcaaaagaat attgggaaaa agcgaaaaaa
     1261 gaattgggcg ttgattcttt agaatttgat ttgatggctt ctgatgatga ttcatcgaaa
     1321 aaagtaatcg aatatgttca aaactcaatc caagaaaact tggatggcgt aacagtcaaa
     1381 ccaacacctg ttccattctc tgtacgtctt gaccgctcaa cttcgggtga ttttgacgca
     1441 gtactaggcg gatgggctgc tgactatgca gacccaagca gctttactga tttgtttgtc
     1501 acaggtaatt catacaaccg tggtcgctgg agcaatgcag aatatgacga agcagttaaa
     1561 gcagcttcat caacagacgc tggcgatgaa caagcccgtt gggatgattt ccaaaaagca
     1621 atcaagatca tctctgatga tatgggtgtc gttccggtgt accaaaaagc agaaggtcac
     1681 ctagtgaatg aaaaaatcaa aggtatcgtt caccatgcgg caggcgcttc atgggattac
     1741 aaatggacat acgttgaaga ataatcgtta ataccgtgag catgagaaaa aacgaggctg
     1801 cggcaaatgt cgcaacctcg ttctcatatt cacttatcct aacaaagaaa aaagcatgaa
     1861 aatagcagaa agagcaatta gtattccggc aatgatcagc caaaagctgt acataccgta
     1921 gctggcagac gagagcgagg aaaattctgg ttttttcttt cggttccttg ttcgtgcaag
     1981 atggaccgat cgctgaaaat gatcgaagaa acccgaagaa aagacccata aaaaaccgcc
     2041 gataatcaga aacggcaatg accataagaa tagatcatta gataattgaa tgagtgtaaa
     2101 ggaatggttg atgagatttt ttattaaaat aaccagaatc agtagaaaag ctactgcgta
     2161 ggggatagat ttttttttca tttgattgct cctttatttt tggttcatta tctaataatg
     2221 tagcgaaaca tctgtcagaa gtcaaaagct agggggaaac attagtgaat agttatatca
     2281 aatatgtatt aaaacgtgtc tttttcatga tcatcacgct ttggttaatt gcaacaatca
     2341 cctttttctt gatgcaattg ctgccgggca cgccatatac gaatcaggaa cgtttgagtc
     2401 ctgaaacaat cgagatgcta aataaacaag tcggtttgga taaaccagtt atcgtacaat
     2461 atgggattta tctttctaat ttgcttcaag gagattttgg aatttccttc caatttaaaa
     2521 accagccagt cgcacatttg ttagctggaa gaatcggacc atccttgcag ttagggttac
     2581 aggcaattat ctttggaaca gtcctaggga cgattttagg aacgatctct gccatgaagc
     2641 aaaatacatg ggcagataca tcttctacac tcgtagcgat attaggtcgt tccattccaa
     2701 actttgtatt tgctgtttta ttacaatata tctttgcaat caaattacaa gttttgccaa
     2761 tcgcaaaatg ggatggcttt gtgtacacaa tcttaccaac aattgcgttg gccatgtcgc
     2821 cgcttgctga ctctgctcgg tttatccgta cggaaatggt ggaagtactg catagtgatt
     2881 atgtggaatt agcaaaagca aaaggattga gccgttggga aatcgccttt aaacatggtc
     2941 ttcgaaatag tttgattcca ttgatgactt tattaggacc gttagctgta gcattgatga
     3001 ccggatcact ggtggtagaa aatatctttg ctattcctgg tatcggggaa caattcgtta
     3061 agtcaatcac aacaaatgat tatcctacga tcatggctgt aacgatcctt tactcattta
     3121 tgctgatttt tgttattttg atcgtggatc tattgtacgg tctagttgat ccaagaattc
     3181 gtgtttcaga agggagccga ggataaatgg aaatgatacc aaagcgttat gaaacagttg
     3241 cagatatccc tgcggatgaa tttcttccat tacaaaaaaa taccgaagaa gaacgtgaac
     3301 agattactgc accttctttg aattttatcc aagattcatg gcgccgttta aagaaaaaca
     3361 aagctgccgt tatttcaatg gttttactat tggtcatcat tttgatctct gtgatcacaa
     3421 tctttgtttc tcctcatgat ccaactgccc aaaacgtggc ttatatcaat ttgcctcctc
     3481 gtattccggg aatcaatatc gatggattga atggaaaagc aatggttggt ggtgaacttg
     3541 ttgataaata tgctcaagca aacgtccctt caaacgtaaa tttcttttta ggaacagacg
     3601 gtttgggacg tgatgtatta agccgcttat ttatgggaat gagaatctcg ttgctgatcg
     3661 catttattgc tgcattacta gatgtaacga ttggtgtagc ttacgggttg gtttctggtt
     3721 tgttaggcgg acgtgtcgat aacgtgatgc aacgtttttt ggaaatctta tcaggaattc
     3781 caaacctcgt tgtcatgatt ttgatgttgg tcgtttttga accaggaatt ttttcaatcg
     3841 tggctgctat ggccatcaca aactggatac caatggcacg tatcgttcgt gcacaaacaa
     3901 tgaaattaaa agatcaagaa tatgttttag ctggattgac attaggtgaa tcaagaatga
     3961 aaattgcatt caaacatatt ttgccgaata tctctagtgt cattattatt caaatgatgt
     4021 ttagtattcc atctgctatt ttcttcgaag cctttttaag ctttatcggt ttaggtctta
     4081 cgcctccgtc agcttcatta ggaacgatgt tgagcgaagg gtacaagaca ttcctgtatc
     4141 tgccttatct attatggatt ccggcagtaa ctttatcggt tatcatgatt ggctttaact
     4201 tacttgctga tggattgcgt gatgcctttg atcctaaaat gaaagagtga agacgatgac
     4261 gaaaatttta gaagtgaaag acttagaaat ctcttttgat acgtatgctg gaaaggtacg
     4321 agcgattcgc ggtgtcaatt ttcatttgaa taaaggagaa acattggcga tcgttgggga
     4381 atctggaagt ggaaaatcag tgacgaccag aagcatcatg cgcttgctgt caagcaacgc
     4441 caatatagat gctggtcaaa tcctctttaa aggtaacgat atcgttggaa aatcagaaag
     4501 agagatgcaa aaaatccgtg gtaaagaaat agcgatgatt ttccaagatc caatgacctc
     4561 tttagatccg acgatgccca ttgggaagca agtcgctgag tcccttagaa agcataataa
     4621 agtttcaaaa aaagagggac aaaaagctgc attagaattg cttaaattag ttggaatacc
     4681 cgaagctgag aaacgtatca acagttatcc gcatcagttc tcaggaggtc agcgtcaacg
     4741 gatcgtgatc gcgattgcac tggtatgtta tccggaaatc ctgattgctg atgaacctac
     4801 aacagcgctt gatgtgacta ttcaagcaca gattttggag ctgctgaaag aaattcagac
     4861 aaaaatcgat acatcaatta tctttatcac tcatgacctt ggtgtagtag cgaatgtcgc
     4921 tgatcgtgtc gctgtcatgt atggaggaag aatcgttgaa gttggaacat cggaagaaat
     4981 tttctacaac ccgcaacatc cctatacttg gggactgctt ggttcaatgc cgacattaga
     5041 tagtgcgaac gatcgattgt atgctattcc tggttctcca ccggatttat tgaacccgcc
     5101 aaaaggagac gcattctatc caagaaatga attcgcgatg aaaatcgatg cagaacaaga
     5161 accaccattt tttgaactat caaaaacaca caaggcagca acttggctac ttgctccgca
     5221 ggcacccaaa gtgacaccgc cagaagaaat ccaacgtcgc tgggctatct ttaaagagaa
     5281 acaaaaacat acttacggtc aaggaggaat tgctgatgcc aaataaacta cttgaagtta
     5341 aaggattgaa acagtatttc aatgtaggaa aaaagaatga agtacatgct gtaaatgaca
     5401 tcagctttca catttatgaa ggagaaacat tcggtttagt tggagaatct ggaagcggaa
     5461 aatccactac tggccgtacg atcattcgct tgaatgaacc tactgatggt gagatattat
     5521 tcgacggtca agatataacg aagatcaaag ataaaaaagg tttgacgaaa ttccggcatg
     5581 atgttcagat gatctttcaa gatccatacg cttcgttgaa tcctcgtatg aaagtcagag
     5641 atatcattgc cgaaggaatt gatgttaatg gactagctaa atcatctgaa gagcgtgcaa
     5701 aaaaagtcga tgatctattg agaactgttg gattgaatcc aagccacggt actcgttatc
     5761 ctcatgaatt ttcaggtggg caacgccagc gtattggtat tgcacgagca ttggcagtaa
     5821 aaccgcgttt tatcatttgt gatgaaccaa tttctgcact agatgtgtcg atccaagccc
     5881 aagtggtcaa tttgttgcaa gatctccaaa aagaacatca attgacctat ctatttattg
     5941 cacatgattt gtcgatggtt aaacatatta gtgaccgaat cggtgtaatg cataacggct
     6001 tgttattaga gatgggaaca agtgatgaaa tctataatca cggtgttcat ccttatacag
     6061 aaagtttgtt atctgctatt cctctaccag atccagacca tgagagaaaa cgcagaagaa
     6121 tcaaatatca acctgaacca gatgacggac aagtacgtca acttcgtgaa atcgcaccag
     6181 aacattttgt ctatgcaact gagcaggaag tcccatatta cgcaaaaaaa ctaaaacgac
     6241 aaaaggaatc tcttctggtt gctaactagg agggacatag atgctaaaag cttttaaatt
     6301 ccgtatgtat ccgacagaag aacaaaaaca gcaattgatt cgtacttttg gatgtgctcg
     6361 atttacctat aaccatttgc tgaaaaagcg gcagaaaagt tggcaacaaa caggagtagc
     6421 caatttttcg ctcacacccg ctacattaaa aaaagaatac ccattcttaa aagaagtaga
     6481 cagtcttgct ttagcaaatg cacagcttaa tttggatcgg gcttttcgca attatttcaa
     6541 gggacgtgcc agcttcccaa agcttaaaac gaaaaaaagg atgtggcaat cttatacgac
     6601 caacaaccaa acccgtacga tctatctgga aaatggctac ttaaagttac caaaacaaaa
     6661 agaactaatc aagataaatc agcatcggcc agttgaagga tcgattcgct ccgctacgat
     6721 ttctgctcga tataatgaag aattttatgt cgcactactt tgtgatgttt cgccagtaaa
     6781 aaaagaatct ttagctaaat ggatcgggat cgcctatcat ccgaagacat tgatccaaac
     6841 atctcgacct ctagaagtaa ctttaccaaa atttcaccag acagaagaaa agctgcaaca
     6901 tgcacagcgg aaattgaatg tgaaagtccg ctccgctcac catagaaaga tcaggttaga
     6961 tcaggcaagc aattatcaga aacaaaagag aaaagtaatg gatctttatc tgaaacaaaa
     7021 gaaccagcga gaagattatc ttgaacaatt atctgggaaa ttggtcaaac aatatgacta
     7081 cttatttgta gaatcttttc caaaagaaga ggcacatgct gatttttcta ttcacgactg
     7141 gcataaatta ataacaaaat tgcgttataa gtcccaatgg tataataaaa aatttctttt
     7201 gatcaatacc gacggagcag aagaaagcaa ttccgtaaga aagagccaag tagtagaaaa
     7261 aatgggcaga cattcggtta tcaagggata aaaaatgaat gaaactgata ggagcgttag
     7321 ggttagcctt gttaataaaa agtcttaatg acttttgttt ctaggaattt ttcttttgaa
     7381 gaaaagctca ctaattatga caaaccaata aaacaatagc agaaaaagtt cgtacgagct
     7441 ttttctgcta ttgttaattt tttattcaga aagtttacga atacatcaaa attctgaaac
     7501 aaaaaacgta actcacaaag agttgttttt gcgttttttc ttgaatgatt aggtctgaat
     7561 cttttgctca caaccttatt catggtgccc cggcttgagc tgatagattt ttgaaggacg
     7621 gccgattcct acaggacgtt caccgatctc atcgaaatat tggagcatag ctttcttaaa
     7681 attggaatga tcgatcgttt tataatcgat ccctaagaat ttagcaaata cttttcgagc
     7741 ttccgtaatc gtgaaatctt tacctaaaac ttgtagaact tgcggctcgt gttccatttt
     7801 attgactaca cggttgaatg ctttcaagat gatttgacta tggtcaaaag ccaaagtatc
     7861 tttgccagaa gactctccag tgactaaatc aagcgagata gccacttctc cgctagataa
     7921 atggatcttg ttactatgac gttctagtgt gaaccagcgt acttccttgg catcatctcc
     7981 ggcgattagc ggttcttctc caataaaggc taagtagctg actgtgacga cccagcctcg
     8041 cggatctcga ttaggcgtac tgaatgtatg cagttgctca atattttctt ttgaaataac
     8101 cacaccagtt tcttcctttg tttctcttaa gacgctttct ccagttgact catccttttg
     8161 gacaaagcca ccaggcaatg cccatgaatt tcgataagga tgcccttttc tttggatcaa
     8221 caaaactttc agctgatctt cttctttgtt atagcacatc agtacgatat cgacagtcaa
     8281 agagggcttg tcatattctg gacgttcctg tttttgatac cagttaagaa attcttcttc
     8341 acttgcttta tgttcataat ataatttttc ttcttcttta gaagcaaaac ggttcatctg
     8401 accacctcca ttgacagtaa aagtactttt taccttattg tagcagactc tgaaaaaaaa
     8461 ccaaggattt acttgaaaaa tctaggttat catttaaata tgaaaaataa atggaagtat
     8521 gagattgaat aaaaagaaaa gcagataaaa cggcaatact ttataccgct cttcacgaat
     8581 ggcaaagaag ccacaaataa cgatatatac gtcaatgata aaacgcaaaa tccaagctgc
     8641 tgtcataaaa ctgttcaaat gcccatggag gttaaaataa ccatacatac gaatataaaa
     8701 atagctgaat attaaatgtg ctaatgaaag aaaagcaata ctaagtgtaa aaaaaagatg
     8761 tttcttctgc atgattgttc cagtccctta ctaagataga tagtctttat tataccacgt
     8821 gtgaggggag aaataaaaag ccgtttttta gtagatatga aacaaaacaa aaacccgcat
     8881 ttattcaacc tattcatcct tttaaaggat agattgaacg tagatagaag tcgtttatcc
     8941 agtctttttc attttgaaaa aaagctaaca gcatgctttt tttatggtat actagcaagg
     9001 aaacgaattc gcatcatgcg ataaaggagg aagagccccg tggcagaaaa ggaaactttt
     9061 tatatcacta cgccaatcta ttatccaagc ggcaaacttc atatcggcaa ctcgtatact
     9121 acgattgcat gtgacgcaat tgctcgttat aaacggttga tgggctttga tgtgttttat
     9181 ctcactggag tggatgaaca tggtcaaaaa atcgaaacaa aagcagaaga actaggtgta
     9241 aaaccgcaag aatatgtaga taaaatggcg gctgatgtca agaaattatg gaaaacgctt
     9301 gatatcagtt atgataaatt cattcgcacg acagatgatt accataaagc agctgttcaa
     9361 aaaatatttg atcgtttatt agaacaggga gatatttact taggtgaata tgaaggctgg
     9421 tactctgttt ctgacgaaga attctttaca gaaacacaac ttgcagaagt gtacaaagat
     9481 gacgaaggta acgtgatcgg tggtaaagcg ccaagtggac acgaagtaga attggtaaaa
     9541 gaagaatcat acttcttccg catgagcaaa tacgcagacc gtctattgga atactatgaa
     9601 gaacatcccg aattcatcca gccagaatct cgtaaaaacg aaatgatcaa caactttatc
     9661 aaaccaggat tagaggatct tgcagtttca cgaacaactt ttacttgggg agtaccagtc
     9721 aagagtgatc cgaaacatgt ggtgtatgta tggatcgatg cattatccaa ttatattact
     9781 gcactaggat atggctccga agatgaaagt ctattcgaaa aatactggcc tgcagatgtt
     9841 cagatggtcg gtaaagaaat cgttcgcttt catacgatct attggccaat catgctgatg
     9901 gcccttgatt tgcctctacc taaaaaagta tttgggcatg gctggctatt gatgaaagac
     9961 ggaaaaatgt ctaaatcaaa aggaaatgtc gtttatcctg aaatgcttgt ggaacgttat
    10021 ggtttagatg cactccgtta ttatttgctt cgtgctgttc cgtttgggtc agatggtgtc
    10081 tttacgccag aagattttgt ttcccgtgtc aattatgatc tggcaaatga tctagggaac
    10141 ttattgaatc gaacgattgc tatgatcaac aaatattgtg atggactcgt tcctgactat
    10201 gcctcacttg tcactccatt tgatagtgaa ctgtctacga cggctgctaa cgttgtcgga
    10261 cgttaccatg atgcaatgga aaaaatggaa ttcaatacag cattggctga aatctgggtg
    10321 ctgatttctc gtgcaaacaa atacattgat gaaacagaac catgggtatt agcaaaagac
    10381 gaagaaaaga aagctgaatt gaatagtgtc atgatccatt tagctgaatc tttgcgtatc
    10441 gcagcgatat tacttcagcc aatcatgaca gaaacgccag agaagatctt caatcaacta
    10501 ggattggact ctgaaacaat gaatcttgaa ggattacact ttggcgaatt tccttcagga
    10561 acaaaagtag ttgcaaaagg tacaccaatc ttcccacgtt tagatatgga agaagaagtt
    10621 gcttttattc aagaaaaaat gtctgaaggt actcaaacaa acgaagatac agtcaaatgg
    10681 gatccagaag aaacagaact tgtttcaacg aaagaaaaac aaatcaaatt tgatgtattt
    10741 gaaaaagtag aactaaaagt agcagaagtc atcaactgtc aaaaagtaga aggggcagat
    10801 aagttgctgc aattccgact agatgctgga gacagccaag accgtcaaat cttgtcagga
    10861 atcgctgaat tttatcctga tccaagcgaa ttgatcggga aaaaagtcgt cattgtagca
    10921 aatctgaaac cacgaaagat gcgtggacaa atcagtcaag gaatgatcct ttctgcagaa
    10981 gcaccagatg gctctttaca agtaatcgaa gcaccaaaat ccatgccaaa tggctcagaa
    11041 atcgcataga aattcaaagg gggtatgaca ctagttgtgt cataccccct ttttccgaaa
    11101 tttgagaagc aattttcgta aattccttct tattacttga agccaatcat ataatacaga
    11161 aacagagaac tatccaaaat aaatcctaga aaaagaagta cgagcttcgc tgatttttgt
    11221 taatttagaa taatactaat aggaatcgta aaaacagaaa aaaagcataa tgataaaggg
    11281 tcgggcagat tttaattgat tcatctggta ctctttttta ttgtccttac attcataaag
    11341 tttgaatgca atgactttat tacatctata tttatatcta acagtagcgt tgatgtgtta
    11401 attataagtt ctatcacttt atgttcaatt agtttgtgct cttatatgtg tgattgaaaa
    11461 tatagataaa aaaacaatca tcggctttga cttcgatgat tgttttcttt attttgtaaa
    11521 aatcctatca cttgatttta tggtaacctt ttatttttgt cttcaaacgg cttggtaaga
    11581 gagcagtaag cagtgcaaat gggatcgaaa agacatacca gaaaatcgta cgtccgatac
    11641 cataatgttg atagagcttt gtcaacatca cgtgtaacaa gatcaaccct aaaagcaagg
    11701 agccagctat aaaccaatga ttatcccaat gcatgacttc actaagtgcc atcactccaa
    11761 aaggatagaa ataaaaatag atcccgacta aggtcatccg ccagttcttt gtaaatgttc
    11821 cgatattcaa taaaaagaaa atcgctgaaa gcaaaatacc gataggcgga aaaagcccaa
    11881 ccaaaaagga atacaacatc ccccacaaga tcatcacagg acctttgatc aatgcaacga
    11941 ttgcgatgaa caaagtcagc aatattagtt tttgtgataa cgttgcaaca gtcattaatc
    12001 caaataaaat taatgtgaac caatgccaat gggactgctg ttgttcttta cgggaaaaat
    12061 cgacccggtt ggataaataa tctgctaatt tcggataaga acgcttaaaa tccataaact
    12121 gttcctcctt cgtggaaaag gtaggtttag tatacgaaaa ataaaaaaga aatacatccg
    12181 tcttaagagg gagtttttca ctgaaagtgg agaaaaaaga atggtaaatt aaaatattct
    12241 cagttatact gaaaattata ccaaatttca aagaagaaag aggatgggaa tgaaaggcaa
    12301 actaaaacga gaaatcaatc tgtttggcgc attagctacc gttatgggaa ccgtgatcgg
    12361 tgcaggcgtc ttcttcaaaa ctgctgctgt tactgctagt actcagtcta caagtttgac
    12421 attgctagct tggcttttgg gtggtttctt aacgatctgt gctggattga cagtggctga
    12481 attagctaca gcaatcccgg aaactggtgg agctgtaaaa tatattgaag ctgcgtatgg
    12541 aaaattgccc agctttttat taggatgggc gcaaagcttg atttactttc ctgctaatat
    12601 tgctgcatta tcgattatct ttgcgacaca attgacgaac ttattacaat tatctaccga
    12661 ttatcttttg ttgattgcag tgatcaccgc tgtttctgtg acagggttga atttgttagg
    12721 gacaaaagtc ggtgcttctg tacaatcagt gacgttgatt gttaaattaa taccaattgc
    12781 tgtgattgtc atatggggat tattgacccc aggacaagga actgtccagt tatttccaat
    12841 cgaagctgga aaagatgtta cttttgtcga aggattgagc agtgcgttgt tagcaacgtt
    12901 gtttgcttat gacggctggt taggtgtcgg tgcaatggca ggggaaatga aacggccgga
    12961 aaaagatttg cctaaagcaa ttattcttgg tttgagtttt gtaacagtcg tttatctatt
    13021 gatcaatttt gtttttttga aaactttgcc gattgatcat cttgccggta atttgaatgc
    13081 agcttctgaa gcatcagacg tcatttttgg aggaatcggt ggaaagctgg tcactattgg
    13141 gatcttgatc tcggtatacg gtgcattgaa cggttataca ctaactggga tccgcgtgcc
    13201 ttatgcgatg gctttagaag acgagctacc tttcagtaaa cagctgacaa agctttcaaa
    13261 gaagtttacg gttccttatg ttcctgcggt tttccagtta gcagtagctt gtattatgat
    13321 gagccttggt tctttcgact ttttaacaga catgttgatt tttgtgatgt ggctattcag
    13381 tttactgatt tttatcggtg tctttatttt aagaaaaaaa gcgccggaat tgccacgtcc
    13441 atataaagta ccactttatc caatcgttcc gattattgcg attctcggtg ctattttcat
    13501 tttaggtatg acgatgatga cgcaaacgaa attagcattg atcggtatag gtgttacctt
    13561 gataggtatc ccagtctatt atcaaaaaaa gaaaaaacgc tcagaagagt aaaatatttc
    13621 aacaacagac agaaaaagta cgaactaggt cctctagatc ttcgtgcttt ttctatttca
    13681 ataaaacaag ttcttctatt ttgatttttg ctacttctgc aagtagtgct tatgtgctaa
    13741 agtaagaatg aaaaggagag ataaacatga tatttgattc tcatacccat ttaaatgctg
    13801 aacagttcaa tgaagatatt ccagaaacga ttcagcgagc acaagaaatg ggggtaacga
    13861 aaatggcggt cgtgggcttc gatacgccaa cgattgaaaa gtctttgcaa ttaagccatg
    13921 actatccaaa tatatacagt attatcggct ggcatccgac agaggctggc agttatacga
    13981 aagaaatcga aaataaatta caagaacaat tgactttacc aaaggttgta gcattagggg
    14041 aaattggctt ggattatcat tggatggaag acccgaaaga agtccaagat aaagtatttc
    14101 gcagacagat tgctatcgca aaagaaatga acctaccaat cagtattcat actagagaag
    14161 cacttgaaga cacgtatcaa atcctaaaag aagaagatgt tcgagatatc ggtggaatca
    14221 tgcatagttt cagtggtgat tatgaatggg caaaacgatt tcttgactta gggatgcata
    14281 tttcatttag cggtgtcgtc actttcaaaa aagcattaga cgtccaagag gctgcagcaa
    14341 atgtaccaat cgaacgatta cttgtggaaa cagatgcccc ttatctggca ccagttcctt
    14401 ttagaggaaa gcgaaatgaa ccaggctata ctcgttatac agtagaaaag attgctgaac
    14461 ttcggaatct tccgacagaa gaagtggctg cccaaacatg gacaaatgcg caccgattat
    14521 ttggtctaag cgaacatgga taagatgaag atccaagaga ttatagtcgt tgaaggaaaa
    14581 gacgatacaa gaaggctgcg ggaagtagtt gatgcagata cgatagaaac aatagggtct
    14641 gcaatcaatg aagagatcct gatgcaaatt gaacatgccc aagagactcg tggagtgatc
    14701 gtttttaccg atcctgattt ttcaggagaa aaaataagaa aaatcattat ggaagctgtt
    14761 cctcaggcaa aacatgcttt tttgccgcgc acacaagcta aaccaaagaa aaagggaagc
    14821 cttggtgtcg agcatgcaag tgatgctgct attttagaag cattgaaaaa ggtcgtgacg
    14881 ccagacattt cccaaacggc tgtttctgaa atcactagac aagacctttt agactatggt
    14941 ttaatagctg gggcacaagc aaaagaaaaa cgagaaaaac taggtgacga gttgcgcatc
    15001 ggttacacga acggcaaaca attggaaaaa cgtttgaaga tgtttcgtat tacgccgaaa
    15061 gaactcgccc aagcaatgaa aatagtggag gaacaacatg agtgaatatc gtgaaattgc
    15121 gacaccctct agaacaaaag agattttatt gaaacatggt ttttcattta aaaaaagttt
    15181 aggacaaaat tttctgactg aaccaaatat tttaaggaaa atcgtagaaa ctgcgcaaat
    15241 agatgaacat acgaacgtga tcgaagttgg accaggaatc ggtgcattga cggaacaatt
    15301 agccaagcat gcaaaacaag tcgtagcatt tgagatcgat gatcgtttga tccctgtatt
    15361 ggctgatacg atgcaaccct atgacaatgt gactattgta catcaagata ttttaaagac
    15421 cgatttgtca acagctgtta gagaaacatt tcatgaggag cttccgttaa aagtcgtagc
    15481 gaatttacct tactatatta cgacaccgat catgatgcat tttcttgaat cagatttggt
    15541 tgtggatgaa ctagtcgtca tgatgcaaaa agaagtagct gatcgtatct ctgctgaacc
    15601 aggaacgaaa gcttatggtt cattatcgat tgctgttcaa tattatatgg aagccagttt
    15661 agcattcatc gtaccaaaaa cagtatttgt ccctcagcca aatgtagatt cagcgatttt
    15721 gaaactgaca agaagagata taccggcagt cgaagtgaca gacgaaaaag catttttccg
    15781 attgacaaaa gcggcatttc agcaaagaag aaaaacgctt tggaataact tgcagcattc
    15841 gtatggcaaa gatgaccaga caaaagcatg gttagcaaaa agcttggaaa cagctggaat
    15901 cgatccaaaa cgtcgtgggg agactttgtc attacaagaa ttcgccgcat taagcaatgc
    15961 gatgtcagaa aatatgcagt aaaaaaagag gttgtgacag aagttgtgaa aaagctgtcc
    16021 tgcatcaagt aataagaaca gccaaacaaa aatagttcct catgtttttc gtttgtttgg
    16081 gcttgttacc gcagatgcaa gcttttgaac accgtttaag aaagaggttg tgggcctgcc
    16141 gtttaactct aagtcacaag gaacaatttc aaaaaacagc ttctcatgtt tttgagttag
    16201 ttggtcttat tacttgatgc aagcttttga ccactattat gccgaacaag gagatgtgac
    16261 acaaattttg tcacatctcc tatttttgtt tcttgatcaa aaaattctta tacttgatac
    16321 tcacactcat tttgtatgga tttagaaaag aatatgtgtc ttgacagtta ttagtaagtg
    16381 agttatacta agtcgacatt tatgtgtcaa gacatattag gagagagaaa aaatgagaaa
    16441 aaattcagtt cgaaaactaa cgatttctgc tttattgatt gggatggggg tcattatccc
    16501 aatggtcatg cctaaaatca tgattccacc agcttccttt actttggcgt ctcatgtgcc
    16561 attatttatt gcgatgtttt ttacaccagg agttgcagtg gctgttgcgc taggtacgac
    16621 atttggtttc tttttaacaa cgccggttat catcgcattg cgagcattgt cacatcttgt
    16681 gtttgcgttg attggggctt ggtacttgca aaagcatccg agtatcgttt taaaaaatgg
    16741 acagtttact tttgctaatt ggcgtttcca agggttcaat tttgtgatcg gtgtgattca
    16801 ttctttagca gaaatgctag tagtcagcat cttttacttt attggtaata tgcctgaaac
    16861 atattattct caaggatatt tttatacagt attcatcttg atggggattg gtgggcttat
    16921 ccacagttta gttgattata atattgctta ttttatagca ggaacactca gcaaatattt
    16981 tgatatcccc gtatttacgg cagcaaaaaa agaaaatcgg aaagagtcga ttcaagggta
    17041 cgcgaagaaa acgacatatt agacatgatc tctcaaaaaa aaaactctca tctagtaatg
    17101 gataataaat cctctaagat gagagttttt tgtcctaaaa agagctgcac caacatcttc
    17161 tggcacagct ctaacagggg gcaatttatg gaagtttgat gacttgttct gtttctttat
    17221 caaagaagtg gcctttgctg atgttgaagg caaggtcgac tatttcgcct ggtttatgga
    17281 aatctcttgc atctactttt gagatgaact cagtagaacc agtttttgtg taaagcatcg
    17341 tttcagctcc aagtaattcg gaaacgacaa cttctgattt cactacggct tcaggtgaag
    17401 cttcaagggc tacttgttca ctgtggatgt cttctggacg aataccgaag ataacttctt
    17461 ttccttcata acctttttca actaatactt tgttttttcc ttcaggaata cgaagcttaa
    17521 gcccatgtcc atctgtgata acaccatttt tcaatgtcac attaaagaag ttcatggcag
    17581 gagatccaat aaagccggca acgaacatat tgacgggtgt atcataaact tctttcggtg
    17641 taccgatttg ttggataaat ccgtctttca tgataacgat tcggtcagcc atagtcattg
    17701 cttctgtctg atcgtgagta acatagatgg tcgttgtttc aagacgctgg tgaagtttgg
    17761 caatctcagc acgcatggct acacgtaatt tagcatctaa atttgataat ggctcatcca
    17821 ttaagaagac ttttgcatcc cgaacgattg cccgtcccaa tgccacacgt tggcgttgtc
    17881 caccagataa agcagctggt tttcgtttca agtattctgt cagacctaag atctctgctg
    17941 cattgtccac acgttttttg atgtctgctt tatcatattt acgtaatttc aagccaaaag
    18001 ccatattatc aaaaacagtc atatgaggat acaatgcgta gttttggaaa accatggcaa
    18061 tatcacggtc tttaggtgcc acgtcattca tgacttttcc gccgatattt agttctcctt
    18121 cagagatatc ttccagtccc gcgatcattc gcaaagtagt tgactttcca caaccggaag
    18181 gaccaacaaa aacgataaat tcacggtctt tgattgatag attaaaatca gttacagaat
    18241 aattttctgc attgtcatat tttttatata cgtttttcag agccatttct accataatac
    18301 gttccacact ttctttttta gttttattaa ctatttaaag tataaatgaa agcgttaact
    18361 aacttcaatg tcatgttgaa caagaatttt ttcttttttt tgtcaatgtg ctagttttaa
    18421 gctttaataa atatccataa gaaataaaaa ctgatacact ggagtaaata agtatgttaa
    18481 caatatcaaa aggagtgtct cttatgaaaa tcgctttgat cgctcatgac cgaaaaaaac
    18541 ctatgatggt gaaattagca acagcgtata aaacaatatt ggaaaaacat gaactttttg
    18601 ctaccgggac gactgggcag aggatcacgg aagcaaccgg cttgccgatc catcggttta
    18661 aatccggacc gttaggcggg gatcagcaaa ttggagccat gatttcggag gatcagttag
    18721 atttggtgat ttttcttcga gacccgctgg ctgcacagcc ccatgaacca gatgtaacag
    18781 cacttatccg tttatctgat gtctatgaga ttccgttagc tacgaatatc ggaacagctg
    18841 aggtgctact aagaggactg gaagccggct tcctagattt tcgaagaatc gtccatgaaa
    18901 tagacaagca tccactttca ttttgatgga taagcgtatg gaaaaataac gtttaatgtt
    18961 cgcctatatg tagctataaa agctaaacaa actgataata tgcgatataa tacgaacgta
    19021 ttggataata taaacaataa tattagtttt tcgtatttgc agtaaatgtg aaaatatgct
    19081 atagtttctg tgggaaaaga tcccaaaaat aatacagagt agaaaataaa gcgttaagtg
    19141 ctaagaggat gggatgttgc ctcttggacg aaggacgtaa tgttcgcggt tttattttcg
    19201 cattcgctgc atgatacgat gtagcagctc ttaaaggaga tgctaaaaat gaagaaacac
    19261 cggttagacg cacgtatgat tgctattatg ggcttattga ttgcattgat ggtcactttg
    19321 tcccgactgg tagcaatcga gaccccattt attaaaatca gtgtcacatt tattccacaa
    19381 gtgatcatgg gcattctttt tggtcctttt tggtcaggga taggcgcagt tttagctgat
    19441 ttagtaggaa tggccctctt ctcgaaatct gcattcttta ttggatttac attgaatgca
    19501 tttattgaag gagcaatcta cggattcttt ttctatcgaa aagagatcac ttggaaaaat
    19561 gccattctgg ctacgcttag tgtcacgctg atcatcaact tatttttgac accattatgg
    19621 cttgcgttga tgtaccatgt gccattgttc agctgggtcg tttgggcgcc ccgcctgtta
    19681 aaaacagtaa tatggttacc gattcaatca attgccattt attacgtagg acgtagtatt
    19741 ccttacaaaa aaattttaag aagtttagcg atccacgcta aataaaaaac aagcagtctt
    19801 cccatcccag aagactgctt gtttttattg tgctgtgaat tgtgcttgat agagccgtcg
    19861 gtaataacca tctttttgaa gcagctcatc atgggtgccc atttcgacaa tagctccctg
    19921 atccatcact aatatctggt ccgcattttt gattgtggac aggcgatggg caatcacaaa
    19981 actggttttt ccttccatca ttgctaagaa ggcttgttgg atttttttct ctgtcaatgt
    20041 atcgactgag ctggtagctt catctaagat caacataggg ggattactga tcatcgttcg
    20101 agcaatcgtc aacagttggc gttgtccatc tgatatcttt aacccttgac cgccaatctt
    20161 agtatctaat ttttccggca aacgttctac aaattcatac atataagagg cttttagggc
    20221 atcattgatt tctgcgtcac ttgcttccgg attcccaaaa cgaagatttt ctcgcaatgt
    20281 actgtcaaat agccaagtat cttgtaacac cattccaaaa cttttacgta atgtatcacg
    20341 ttggatagag cggatgtcat ggtcatcgac tgtaattgtt ccttttgttg tgtcataaaa
    20401 acgcatcagc aaattaacaa gtgtggattt tccggcccct gtttttccta ctatagcaat
    20461 ggtttgacct ggataagcag taaaattgaa atcttcgatc aaacgctgct tggggtcata
    20521 agaaaaagaa acatgatcaa aagagacttt tcctttgata tttttgggtt ccaaggcata
    20581 ggcatctggc aaatcaggct tttcttctgg ctgatcgatc aaatcaaatg ctcgttctag
    20641 accagaaaaa gcggtttgca gttgtgtcgt gattccagaa agttcgataa atggtttaga
    20701 aaactgactt gagtagatcg taaagctgga aatcacacca acagtgattg acgaaccact
    20761 cttcaatgcc aataggccgc caactaaacc aatcgataaa taagcaaggt gatctacaaa
    20821 acgagaagac gggtttgtca aggaagaaga aaattgtgcc cgttgtcctt tttcatatag
    20881 atcagcattt agcagttcaa attgctgttg actagcttct tcttgctgaa aagctttcac
    20941 gattttctgg ttaccgattc gttcggtcac aaagcccgag atatccccaa taatcctttg
    21001 ctgtctatcg aaatttgttt gtgaagcacg agcaacgatc caactcacaa ggaaaataat
    21061 tggtgtagaa caaagaacga ccaatgtcag aagtggactt agacgcatca taaaaataaa
    21121 cgaaagaatg atgattgcca ctccagaaaa taattgattg aaggccgcag aaattgcaat
    21181 ggaaatattg tccatatcat ttgtaaatcg actgacgata tttccgtggg agttttgatc
    21241 ataataacga agtggcaatc gattcagatg agcaaatgca tcttttctta attttgcaac
    21301 ggataagtaa gctaaccgat tgcttaacac ttggatgagc cattgactga ctacgttgat
    21361 taaaacgata ccaccgaata gaataagtaa atggaacagt cgattgaagc ggacctgtcc
    21421 ttctccaatc atttgatcga tactttgacc aatttgtaac gtcatcacaa ctgttgcaat
    21481 cccattgatg attcctagta tcacagctag ccaaatgtct tttgcatagg cagtaaggta
    21541 aggaatgaat cggcggagcg tctgcaagga taattttttt actttcataa ctattcttcc
    21601 tcctgtgatg cgacgatttc ctgatattct gctgattgag caagtaattc ttcatgggta
    21661 ccctgagcag caatcttacc ttgatccatc actaagatat gatgtgcttc ttgaatagag
    21721 cggacacgtt gggagataat aatcactgta ctgccaagat aagtctgtag cgccttacgc
    21781 aaatccaagt ctgtttgata atccaacgca cttaaagagt catctaaaat cagtaatgct
    21841 ggcttggcaa tcaaagctct tgcaatcgtc agtcgctgtt tttgtccgcc agagaaattt
    21901 ttcccatttt ccatgacttt tgtatctaaa ccatttggaa gctgacggac aaattcttct
    21961 gcctgagcga ttttcagagc ttcccaacat tctttttctg tggcgtcttt cttaccccat
    22021 tgaaggtttt cacgaatagt cccggtaaat agtacagaag tttggggaac aagagcaatt
    22081 tgctgacgga gatgatccag ctgccaagaa cgaacatcca ttccgtccat tataactgta
    22141 ccggaactga catcatagaa acgcggaatc aattgtgtca aagtcgtttt cccgcttccg
    22201 gttggaccaa tgattccaag gactgtattt cgcttcagct gaaatgtaat attttggaga
    22261 gaaagaccag aatcaggagt ataacggaag tcaacatggt caaaagcgat aattgcttca
    22321 gaagaaaaat cgggagtagt actgacctca gcttcaagaa tcgaattttc tgtgtctagc
    22381 acctctttca cccgatttcc tgaagcttcg gcacgtgtga agatcacgac gaggtttgaa
    22441 acaacgatca gtgccaaaag catttgattc atataattga tcaaagcaag aacttgtcct
    22501 tgttgcaagc taccaatatt tactttgatc ccgccaactg tcagcaaaca gatgatcccg
    22561 acattcatga tcaaggttgt agcaggtgat aatagggcag agatattgga aacacgtaaa
    22621 taattgttcg ctagatcatt tgtgctttta tcgaacttct ctgtttcttt ttttgtctgc
    22681 gcaaatgcgc ggatgacacg aacaccgcta agattctgac taacgtgttc attgagctga
    22741 tctagttttt cttgcacttt ttggtagaga ggaacggttt ttttgatgat aaaaaataaa
    22801 atgattgaga aaataggcaa taatgctaag aaaaacagac ctacttccca gtcgatcaca
    22861 aaagccatga ccactgaccc gatacttaaa aacggtgctc tgatcaataa acgaatgacc
    22921 attgctaaag ctagctgaag ctgattgatg tcgtttgtca ttcgggtgat caaggtatct
    22981 gttccaaaat gattcaactc gttgtgagag aaagtattga tttttttgat tagctgatta
    23041 cgtaactctg taccaaatcc ttgtgaagct actgaagcat agtattggca aatgatagca
    23101 caaatcagac caatcacaga catagcgacc atccataagc ccatttcaat gatttttcct
    23161 cggtcattca tttttagtcc attatcgatc aaggaagcca tcattaaagg caggacaagt
    23221 tcgaagacag cttcaagaaa tttgaagaac gggcccaaaa tgatttgctt acgatagtct
    23281 tttgcataac gtaataactg aatcatatga ttcctcctat tatttatttg tccacaattg
    23341 aaaaaacaag tactaccaca ttgtagctta ggaaatttag aatgaaaaga gaacaagcct
    23401 tttcataaat gaaagtttta ttatgaagaa gctttagaaa ttgaatagac tatttttttt
    23461 aaaaaaaaga aaatgagaaa acatgccagt ctcttcattc ataggaacgt ctattttact
    23521 taatttttct ataaatgaat tctgcataaa aaatatatca agtaaatgtt tatagatgaa
    23581 actctattta cttctttttt aatttgttct tgcaatcttt tttgctacgt aatttttgta
    23641 aaaaagtggg ttgagagaaa tctaattgat tttcaaagaa ggagtatgaa aatgagaaaa
    23701 atatttattc gtttattgtg ggtactatct tagaggaata aataatagtg gagaaaacta
    23761 tggtttggcg cagattctat caggtttgat ggaacgaaga caacctcccg acatcaagtc
    23821 aaaagctact tgagattttg atctgaaaat aaaaaaagaa aacaaggtag gaacatcaat
    23881 gtttctacct tgttttcgta atcaatggga aaaaactaag agtggtgtac gtaatcaata
    23941 aaaaggatgg tgacaacagt acctctttac tctctgaaag catcataact cgtttttcct
    24001 cattatgcca ataaattgtt gttataccac atataaggat ttgtaatagc taaaaacaga
    24061 caggaaagtt gaaatctgtg aatcttattt tgtatggaag gatttataga acgcaaaaat
    24121 caagataaag atgaataaaa atcctgtttc ggctatatat tgcgtatcta cgccaataaa
    24181 acgagtttgt tctgtcatag ccatataaag caaaggcaaa aatacaacta cagcgagata
    24241 agatccgatg atttctcgaa aagttttcca ggaagtatgt ttcctgtttt tatatttatc
    24301 cgatttcatt gaatagtcat agcctcctta ttctttcata tgttgaaatt ccagtataaa
    24361 aactggaagg aactgtacgt tttttagaat aaaaattata agggataagt aggctgctgt
    24421 ctataataag atatattttt caattcttta ttaaagattt tattgacaaa atgaatccgt
    24481 tttctatgat ggaggagggg tgaataagtt gttgaagaag gaaattttat gttacttaga
    24541 gaaccagaca gcctttttcg atttagaaca tatgaacgag gtctttacag cacaaaggtt
    24601 agctgatcat tttggggtaa aaagaaacac agtcagtcat tatttgaacc agttaaatga
    24661 tgagcaacta ctagtcaaaa tcaattctcg accagtgatt tatttccaca aagaagcttt
    24721 tgaaaaacaa ttttttaagt tacgcacgaa tttttatttt tctatcgatg atctcaaaga
    24781 ggaacagcct tttttcgcgc aaccaaaaga cttattctct tttatgatcg gatacgacgc
    24841 cagtttgaaa gaaagtatcg aacaaatcaa aacagctctt tactatccag atggcggatt
    24901 accacttttg attacaggcg aaagtggcac aggtaaaagt tatttagtta atttggtcta
    24961 tcagtattgt ctgcttcatg atctgctaga agattctgca ccattcatca cttttaattg
    25021 tgcacaatat gcagataatc ctgaactttt gactagcaat ttgtttggac atgttaaggg
    25081 agcgtacact ggtgcagaag aaaataaaaa aggggctttt gaagcagcag atggtgggat
    25141 cttgttttta gacgaggtac atcgcttaac accagaaggt caggaaaaac tgttcaccta
    25201 tcttgatcaa ggtgtgattt atcgtatggg agaacctaat ttgtcacgcc ggatcaaaac
    25261 acgtctcttt tttgctacga ctgaagagat tactagtcat ttcttaacaa ctttcattcg
    25321 aagaatacct atccgaatcg aactacctgc acttaatcag cgtagtcgta atgaacggct
    25381 agaactgatt tactcttttt ttctcgaaga acaaagaaaa atggggagcc ctttgtcagt
    25441 cagtggacca gctttgtcac ttttaacaag tcagaaatta tccggaaaca taggagagct
    25501 gaaaaatatc gtcaaagttt cagcagcaaa agcatatgct gaacagcgtg aacagcctga
    25561 aatccatgtc acgatccatc atttgcaaaa agaactactt gcgcagccta ttactaaaaa
    25621 tacagcacag caaggaatct atattacgca agaccagacc ttagagcaat taatggagaa
    25681 gcaacagccc gaacagcagc gaatcgttaa aagttttgag caaatcctaa ttgatttcaa
    25741 gaaaaataac tgtctgctat attcttgcga aggaaaatta aagcaagaag tgattcaatt
    25801 atttgatttt ttgctgtttg agaccagtag acaagaaaaa cacgagctgc tggtttattg
    25861 gacacaatct atccgagaga cctttcgaca aatggaaact gcctatcaaa tcaaattcga
    25921 tggaaacagt gtctatgcct tgagttacta tctctatcag cgaagcacta tcaaatggtt
    25981 accggaagaa catgatatta tccaactaat caaagaatta gaacaacaag tttctcagtc
    26041 ttatccatct agttatcact atgtccaacg aattctggaa ttaagtaaga tcaaattaga
    26101 tattgaagac acaagtatgg atcggatttt actgacgatc tatctaaaaa aagcgaaatg
    26161 gtcaaaagaa aaacgactgc caaaagcgat gattgctgct cacggttatg caactgctag
    26221 cagtatggca aatgtagtaa atcgtttatt acaagaggat ctatttgaat catttgatat
    26281 gccactagat gtcacaccac agcagattgc ttcagaaatc cttgattatt gcgaaaacag
    26341 cgatgtttca aatggattga tcatattagt agacatggga tctctgaaag aaatccatca
    26401 gttttttaaa aaacagttat ccgtaccgct tttgattttg aataatgtga cgacaccttt
    26461 agcaatcaca gtaggggaat gcctgcaaaa aaatgtatca ttagaagaga tagcagaaga
    26521 gactgttcga cagatccagc cagaatggcg actgctctat ccagaggaaa acaaacccaa
    26581 agcactcatt actacttgct tcactggaat cggtacggcg gtccacttaa gtgaactgct
    26641 agaaaaaagc ttacctacgg cttgtcagtt gaaaatcatc ccatatgaat atcagcaatt
    26701 aaaagataag aaaaacagtg acccattatt ttccatatac gaagtcgtag gaatgattgg
    26761 caccacagat cctgatatca ccaatatcga ctatctttca ttagaagacc tcatctcggg
    26821 ggaaaagatg tctgtactag ctgaatggtt ggacactact atgaatgctt cagagaagga
    26881 aatattcaat cagcgcatca ttcgtaattt ttcattggaa aaagtactgg attccgtgac
    26941 tattctagat acagagaagg tgatgggaga aatcgatcat tttatgagag aattagaggt
    27001 gcagttgaat cgaagcatca gcaatcccaa aaaattagcg ttgtatgtcc atgtgagctg
    27061 tctcattgaa cgattgatcc gacaaatgcc aatcgaaacc tatcaaggat tagagaaatg
    27121 gaagcagtgt caaaaagaag ggttatctgc tattaaatct gcgtttagtg tcattgaaga
    27181 gcattatagt gtcatgatcc ccaattctga gattgcctat atctatgata ttttgagtga
    27241 agatacagag tttctctcga tagaagaaga attttgaaca taagcaagtt gttgccacta
    27301 attttggcat gcaacttgct tttttagtat gtgtaagtga aaagaggaga tgaaaaagtg
    27361 gatagagaaa ttatcttagc ttcccatgga aaatttgctt cagggatatt agattcatta
    27421 gagctgatct atgggaaaaa tcattctatt actgtattcg attgctatac tgatgagaat
    27481 tttgatttag cggaaacagt aaggcagctt tttatgaggt acaaagataa agaaatcatt
    27541 gttctgacgg atctttttgg cggaagtgtc aacaacgaat tcttacaata tatcaatcgg
    27601 gatcatgtct atctcgttgc cggattgaat ttgccactat tgattgagct agtatctcgg
    27661 ttggacgctg aagtagagac aactgttttg atccagcagg tgctaactga ttcaaaaaaa
    27721 atgatccagt tttgtaacga gcggctcaat cagaaaatag aagaagacga attttaggag
    27781 gaagaaaaat gattgtattg acaagagtcg accatcggtt attacacgga caagtagctt
    27841 tttcatggac acaaacaata ggcgcagact gtattttgat tgctaatgat gatgtaccta
    27901 cgaatgagat acgaaagaca acgatcaaat tagccaagcc acaaggagtc aaactagtga
    27961 tcaaatcaat tgatgactcg attgccgcgt tgaaaagcgg ggtgacagat aaatataagt
    28021 tattcatcgt agtggaatcg atcgaggatg catataagct ggcagaagcg tatccagaaa
    28081 tcaaaaccat caacttagga gggatcaagg caaaacctgg tactcgcagc atcggaaaag
    28141 cggtaaatat tttaccagaa gaggaggaac gcttgaaacg attaattgac aaacaaatag
    28201 aagtagaaat ccgtcaagta ccagcagata aaaaagtgga tgtaagagaa gttttgtaag
    28261 aaaggaagaa cctcaatgat tgtacaagca gtcttattag gaattgttgc atttgtcgca
    28321 caatcagaat atgcactagg gacatcgttg ctttctcgtc cgattgtgac ggggctgttt
    28381 acaggaatcg tgttaggtga tgtaaaaact gggatcatta tgggcgcaac tttagagtta
    28441 gcatttatcg gatcgttctc agtaggtgct tcgattccac ctgatgttgt gaccggtggg
    28501 attttaggga cggcttttgc tattacagct ggagccggta cagaaaccgc gcttttacta
    28561 ggtttgccga ttgctacgtt gacattgata ttgaaaaata tttacttagg gcttttgatt
    28621 ccaattatga accataaagc agacaattat gcagaagaag gaaattataa aggagtcgag
    28681 catatgcact tgcttgccgg tatcggtctt tccttgatgc ttggtgttgt ggtaatggtt
    28741 tcttatatgg tgggaagcaa tacgatcgga aatctattaa atatgattcc tgacttcgtt
    28801 caaaaggggt tgtcagtagc tacagggttg attccagctc ttggttttgc gatgcttgct
    28861 cgtttgctga tcaacaaaca agttgcacca tattttttct taggttttgc tatagcttct
    28921 tacttagaga ttcctgtgac cggagtagct atttttggtg cgattttagc agttgtggta
    28981 gtcaatatta tgaatgtccg tcaactacca cttcaaacaa ctgcagggga ggtagaagaa
    29041 gatgaagaat tctgagatca aaaacagcaa aataacgaaa aaagagctaa atcacgtatt
    29101 ttggcgttct tttcaaatgg agttttcttg gaattatgaa cgtcagatga atatggctta
    29161 tgtttatgca atgattccga ttttgaaaaa gttatatcag tcgaaagaag aaatggcagc
    29221 agctttgaaa cggcatctag agttttttaa cacgacccca catatcgtta cactgatttt
    29281 aggaatcacg gctgcaatgg aggaagaaaa taaggatgat cctgaatttg atgtgactgc
    29341 gatcgatagt atcaaaacat ctttgatggg acctcttgca gggttaggag attctttctt
    29401 ttggggaact ttgcgattga ttgccacagg tgtgggtacc tctttagcca tgcaaggaaa
    29461 tattttaggc cctatcttgt tcattttgat tttcaatatt ccgcacattc tgtttcgcta
    29521 tctagcaaca ggttggggat atcgcttagg aacgggtttt ttgaaaaaga tccaggcaaa
    29581 cggtatgatg gaaagtttga cattaggcgc gtctattatt ggactgatgg tcgttggagc
    29641 aatgactgcc acgatgatca ccatcaatat tcctttaaaa atcggttcag gagaaaatgc
    29701 tgtgactgtt caaagtattt ttgatgatat cgttccaaat atcttatgtc ttggagcatt
    29761 tggcatagtg ttttatctat tgaagaaaga agtgaaacca ttaacgatct tgatcggact
    29821 agctttcttt ggaatttttg gttcgtggat agggattttt taaaaaagga gcagagaaaa
    29881 tgtttacgat gcaagattat atctatgaag aaaaggaagt actaagtacg atcttgaaaa
    29941 agaatgattt ttcgacaaga gaacatatga aaaaaacagt gaatttactg atcttagcaa
    30001 cgggttcatc ttataacgct tgtttagcag cgaagccagc attggaatct tatggtgatc
    30061 ttactgtcga tattcaagaa cctttttatt ttttcaatta tggaaaactt tctccttcaa
    30121 tcgatactgt gattgctgtg tcacaaagcg ggaaaagtgc ttctactgtt gaagcagtaa
    30181 aaatgataca aaaacaaggg ttacctgttg ttgctatcac aaatgacgtg caaagtccac
    30241 ttgctttaga agctgcacaa attattgatt taggtgccgg ggtagaatca gtaggcttcg
    30301 ttacaaaagg ctattctgcg actgttttac agttactact cttagggata ggaattggaa
    30361 taagtaaaaa taaaatcagc aaaaaaatag aacaagacta catgcaacag ttgcgcaaaa
    30421 taatcaacca tttaccagcg attattcaaa aaacggaaga attttttgat ggatatcaaa
    30481 gtttatttcg tttagcacag cgtttcgtca ccattggata tggtccaaac tggggaacag
    30541 ctaaagaagc agagacgaaa ttgactgaaa caatccgcgt tccgtcacaa ggatttgagt
    30601 tagaagctta tatgcacggc ccatacctgg aggcagatgc ttcgcatttg ctattcttta
    30661 ttgaagggga aagtgtaaat aaggaacgtt cgcaaaagtt gcaaagatat atgagtcggt
    30721 acgttggaga gactctcacc attaccacaa aaaaagcaag aaacgagaaa acacttggat
    30781 tagcaataga atgtgatgaa tatctgtctg tattagcgct tgtcgttccg tttcaactat
    30841 tcgcttataa aaccgcagta gctaaaggta ttgacttgaa caaaaagatt tttgaagact
    30901 ttgatacagt cttgaaaagt aaattataag aggtggaaat catgctgaaa ttcaatgaag
    30961 aagaacaaat caaagcaata aaaggcgcac tagctttacg tccgcaagta gaagaaatca
    31021 tcgataaact ttatgcggaa aaatttgacg ctgtctatta tctaggaatc ggtggaacgt
    31081 atgcttcctc gatgcaagct gtcacttata tgaatgggaa aagcaacttg cctgtctttg
    31141 tacagcatgc tgcagaatat tacactactg gaaacaaaag attaacaaaa gattctatcg
    31201 ttattttgtc ctctgttacc ggtacaactc aagaagtagt gaaagcagta gaacaaatca
    31261 agaaagtagg agcgacactg attggattta tcgataaagc gaacagtaag ttgtctcagc
    31321 tatgcgattt tgtggtcact tatccagctc ctggaacaga acaaatcaaa ttttttatgg
    31381 cagcagatcg attgatgtat ttgaatggag aattcgaagc ttattcagaa tactatgaac
    31441 agctcgaacg ttatttacca acagggttag tggaagcaga gaaaaaagca gatgcatttg
    31501 gcttatcatt tgcagaaaaa catcgacgtg atagtatgca ttattttatt ggagcaggga
    31561 atcaatgggg agctgtttat tcatatgcca tgtgttactg ggaagaacaa agctggcttc
    31621 catcgaaatc tatccatgct gccgagtttc ttcacgggac gttagagatc gtagaagaaa
    31681 caaccccagt cacattattt ttaggcgaag atgaacagag agtgttatct gaacgtgtgg
    31741 ctaaactttt gccaaaaatt tgcagcaatt atacattgat agacacaaaa gactatccag
    31801 tagaaggtat cagtgaaaaa tatcgcggac gtgttttgtc tttcttattg atgcatgtag
    31861 tgacacaacg catcgatgct catgtggagc agttgaactg ccatccatta gagatacgcc
    31921 gctactatcg tcagtttgat tattaggata gactggataa aaagaatata aaaaataaag
    31981 agagcagcga taactgctcc ctttattttt ttaaaatatt ggcatcaatt acgttataaa
    32041 acacattttt tgcattatct acagttcaac ttataatctg tttaatggga catgtgggtg
    32101 ccaaccattt tttgttatgc aatagtacta tttagttgtt gcatggatcc gtagtaaaag
    32161 tccaagtaca acgagttgcg ccagtttaca tagcgttttg tatccagaat gttgactaaa
    32221 acaaaagttc ctatgtcaac cgtcaacaga gacaatctgt ccgttgcagg acctgacaga
    32281 tttagaaatg ttcaatctgt ctcttatgta ttttaaatca atactcacta ggagaagttt
    32341 cctaacctcc taatagtaat gctggtagta acattgtcaa aactgcttcc tcttaggaac
    32401 aagcaaagca taacgagtgt aaaaataaaa tgtagactat tgtaacatat cattgattat
    32461 ttcaagtaaa aacgataaaa gaagtaaaca aacacttaaa aacaatggaa attagtccca
    32521 actttaagaa atgtatacta taatggaaat tatcaattat ttatttggag gaatatccat
    32581 gaaacaaaaa atcgcagttc ttggtcctgg atcttggggc actgctttag cacaaacatt
    32641 ggcagaaaac gggcatgatg tccgtatttg gggaaatgta cctgaacaga ttgatgaaat
    32701 caataagtat catacaaatc agcatttttt acctgatttg acgatcccag aatcgattat
    32761 cggatataaa gaattggcag aagcagtgga taacgcagat gctattttat ttgttgttcc
    32821 aacaaaagcg attcgttcag tcgcacagga acttattggt aaaatgaata ctaagccaat
    32881 cattatacat gcaagtaaag gactagaaca agatacgcat aaacgaatct cagaagtttt
    32941 ggctgaagaa attccagaag aaaaaagaca ggcaattgtt gtattatctg gtcctagcca
    33001 tgctgaggaa gtggcagtac atgatatcac aacaattaca gctgcaagta ttgatcaaaa
    33061 agcagcagcc tatgtacaaa aactgttcat gaatgattat ttcaggattt atacaaataa
    33121 tgatgtgatt ggcgtagaaa ctggtgcagc actgaaaaat atcattgcaa ttggtgccgg
    33181 cgctatccat ggattaggat tcggagatga tgcaaaagca gcaatcatga ctcgaggatt
    33241 ggcagaaatc agccgtcttg gtgtcgcgat gggtgccaat cctctgacat ttatcggttt
    33301 gagtggtgtg ggggacttga tcgttacttg tacaagtgtt cattctcgca attggcgtgc
    33361 cggtaacttg ttaggacagg gacataagct ggaagaagtt ctagaaaaca tgggaatggt
    33421 cgttgagggt gtggcaacga caaaagcagc agtagagcta gctcaacagc taggtgttga
    33481 gatgccaatt actcaaacaa tctataatgt tttgtataat ggagaagata tcaaaaaagc
    33541 agcaaaagaa atcatgttac gtgacggaaa aatggagaat gaattttctc ttcgttaaat
    33601 agatgatcaa tagttaatcg t
//
LOCUS       DAYYFU010000027        32178 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000027 DAYYFU010000000
VERSION     DAYYFU010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.027"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(407..766)
                     /locus_tag="ACHA34_001478"
     CDS             complement(407..766)
                     /locus_tag="ACHA34_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFM8792125.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="ACHA34_001479"
     CDS             complement(796..1137)
                     /locus_tag="ACHA34_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792126.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="ACHA34_001480"
     CDS             complement(1134..1808)
                     /locus_tag="ACHA34_001480"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8792127.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="ACHA34_001481"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="ACHA34_001481"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFM8792128.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="ACHA34_001482"
     CDS             complement(4322..5512)
                     /locus_tag="ACHA34_001482"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8792129.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="ACHA34_001483"
     CDS             complement(5533..6060)
                     /locus_tag="ACHA34_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFM8792130.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="ACHA34_001484"
     CDS             complement(6107..8896)
                     /locus_tag="ACHA34_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFM8792131.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="ACHA34_001485"
     CDS             complement(9043..9243)
                     /locus_tag="ACHA34_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8792132.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="ACHA34_001486"
     CDS             complement(9696..10016)
                     /locus_tag="ACHA34_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792133.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10061..10204)
                     /locus_tag="ACHA34_001487"
     CDS             complement(10061..10204)
                     /locus_tag="ACHA34_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792134.1"
                     /translation="MKFRLISEEATSGTPFKKEVVTWSRPLFDDYDTKNIIYEQNVNN
                     GRS"
     gene            complement(10519..11637)
                     /locus_tag="ACHA34_001488"
     CDS             complement(10519..11637)
                     /locus_tag="ACHA34_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792135.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            11870..11992
                     /locus_tag="ACHA34_001489"
     CDS             11870..11992
                     /locus_tag="ACHA34_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792136.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            12108..13415
                     /locus_tag="ACHA34_001490"
     CDS             12108..13415
                     /locus_tag="ACHA34_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFM8792137.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="ACHA34_001491"
     CDS             13536..14735
                     /locus_tag="ACHA34_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8792138.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="ACHA34_001492"
     CDS             complement(14762..16030)
                     /locus_tag="ACHA34_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFM8792139.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     regulatory      complement(16086..16152)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(16200..16841)
                     /locus_tag="ACHA34_001493"
     CDS             complement(16200..16841)
                     /locus_tag="ACHA34_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFM8792140.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="ACHA34_001494"
     CDS             complement(17250..17753)
                     /locus_tag="ACHA34_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8792141.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="ACHA34_001495"
     CDS             complement(17876..18640)
                     /locus_tag="ACHA34_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFM8792142.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="ACHA34_001496"
     CDS             18748..19023
                     /locus_tag="ACHA34_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFM8792143.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="ACHA34_001497"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="ACHA34_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFM8792144.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="ACHA34_001498"
     CDS             complement(20579..20980)
                     /locus_tag="ACHA34_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8792145.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="ACHA34_001499"
     CDS             complement(20994..21803)
                     /locus_tag="ACHA34_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8792146.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="ACHA34_001500"
     CDS             complement(21790..22680)
                     /locus_tag="ACHA34_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8792147.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="ACHA34_001501"
                     /pseudo
     CDS             complement(22693..23172)
                     /locus_tag="ACHA34_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(23348..24289)
                     /locus_tag="ACHA34_001502"
     CDS             complement(23348..24289)
                     /locus_tag="ACHA34_001502"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFM8792148.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="ACHA34_001503"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="ACHA34_001503"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM8792149.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="ACHA34_001504"
     CDS             complement(25274..26470)
                     /locus_tag="ACHA34_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8792150.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="ACHA34_001505"
     CDS             complement(26488..27216)
                     /locus_tag="ACHA34_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8792151.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="ACHA34_001506"
     CDS             complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="ACHA34_001506"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM8792152.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28628..29086)
                     /locus_tag="ACHA34_001507"
     CDS             complement(28628..29086)
                     /locus_tag="ACHA34_001507"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM8792153.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29083..30546)
                     /locus_tag="ACHA34_001508"
     CDS             complement(29083..30546)
                     /locus_tag="ACHA34_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8792154.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="ACHA34_001509"
     CDS             complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="ACHA34_001509"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM8792155.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31602..>32178
                     /locus_tag="ACHA34_001510"
     CDS             31602..>32178
                     /locus_tag="ACHA34_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8792156.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         9385 a         6921 c         5511 g        10361 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgccatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcagcaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttatcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt
    27541 tattattagt tttagataac ttttgcagtt ttatactttt tttctataaa atttgacaaa
    27601 cattttttta aataactaat tattttttga atctgtcact cagacaatcc tttctcttac
    27661 ttgttaacat aaatagaagt tgctgtttct tctaatagtg tacttaattc tttaatattt
    27721 tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggt
    27781 gctactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat
    27841 tgttttgcaa aacgcaatgt atcttgaaat agtgcagcgc tgactcccgc acttaaaaaa
    27901 ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga
    27961 ctataacaag tatcttgccc atatccttcc acaaatctca tattcactgg tacttctact
    28021 ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc aatcacttta
    28081 tgcggtttta cttttgcata gtcaagagaa gacggatcgc caatagttgc atcgtaagaa
    28141 aggagctcta aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat ccgttctata
    28201 actatttgtt tttgcacatt aatctctact ggttcatcaa cgtcatagta caataaaaat
    28261 ttacaagcat ccgcacccac ttcttttaac cttttcactg accaactatt cagtaaatct
    28321 ggtagtcggc ctggcatttt cgcatcatag ccggtttttt cataagctag caacaaacca
    28381 cattctttct ctcgaagcgc tgctgcaggc agaccatatt ctggatcgag taaaatagcg
    28441 gaagcatacg gagtcaggta ctgagaaacc atctgtttaa attggatgat atcttctgat
    28501 gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgttggtc gatagctagc
    28561 gcgctaataa ttccagcttc atccgtcaat cgcttcaaac gttcctgttt ttctatactc
    28621 attttcttca tatatcttca cctatttctt tagttaatat tgtagctatt tctatttttg
    28681 attttgcagc tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag
    28741 acaataattt caaatgagtc gttcctgctt ctgaatctgg gataaataac gcaaaaataa
    28801 aatcaactga ctgtctatct aaactgttcc attcaattcc ttttttcagt gtgacgataa
    28861 tcaaactagg ttcattgatt gtttgagact tcgcatgagg gatagcaaaa cctgacatta
    28921 ttcctgtagt accttctttt tctctttcca gaagtttttg atatacttct ttttcgtcat
    28981 gagcgattcc taaacagaat gtttgctttg ctaaaaactt aaatgcttct tcttgattat
    29041 ctaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata aacctcctgc
    29101 tcttccttct tcgataactg tacgatttgt tgctttatta tccctcttct tgtctttatc
    29161 agttactctt ttcttttgga tcaaacctag tatcactcca ccaacgatcg aaccaataag
    29221 aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa aaccgccatg
    29281 tggggctggt acttggacac caaacagata ggtcaagaca gcagcaattg atgagcctaa
    29341 catcatcgtt ggtaatactc ggatcgggtc tttcgcagca aacggaatag ctccttccgt
    29401 aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat tagtatcaaa
    29461 atatttacca aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa tgcaagctgc
    29521 tgacgcacca gccataaaag tcgtgtttcc ttgtgtcaaa agagctgttc ctgttacata
    29581 tgccgcctta ttaaccggtc ctcccatatc aaacgcacac atacagccaa caatcacgcc
    29641 aagaacaagt ggattcgaat attcaaatcc agctaaaaag ctcatcattc cttcatttat
    29701 agccttcatt ggacctgcca gcaaagacat cacgatgcca ataaatgcta caccaataac
    29761 tggatataag acgattgcct tcaagccatc tagttgttta ggtaaaatta tcagtagctt
    29821 ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaatacctc ctaaaaatcc
    29881 agtaccgcca ttcatggcta cgattccacc aacaaatcca acgactaacc ctggacgttt
    29941 cgcgatccct tctgcaatat aagctgctaa aactggaacc atcattccca ttgctgcatc
    30001 gtcaactgct tttaccattg ccgctgtagc attgtattgt gcatgatctg gatcagaaga
    30061 atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg
    30121 caacatatga gagacaccgt tcatcaaatt tttatagatg ctacgaccaa aattagcacc
    30181 acttatcgct tctggttcta aagtatcagc agcttcatta tctgctaaga cagtaccttt
    30241 tcctgccaat gcttcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac
    30301 atcaatgact cgttttccca caaatctttc tgaatggaca tctttgtctg cggcaataat
    30361 gacagcatct gcctgtttga tttcttcagc cgttaattca ttctcgatcc caatttgtcc
    30421 atgggtctcc actttgatcg tgatgccttt tttcctagct gcttgctcta atgcctcttg
    30481 cgccatgtag gtgtgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt
    30541 tcccatcttt cgaacctctc ttccttgatt ttgatttgtt tacttaattc ctccacatcc
    30601 aaaaaatcag tcagtccttt acgaaaagca gtagagctgc ctgctgctat gcttttacgt
    30661 aacgtttcat caagcgatcg tcgattcatg tatcctgcta aaaaagtgcc taacatggca
    30721 tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctcg aatcaccctg
    30781 tctttcgtaa aaagaatcgc tcctttctca ccgagggata acaaaacatt ttctgcgcct
    30841 gactctacta gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg
    30901 aaccaagaag aaagttcttc ctcatttggt ttcagcaaaa acggtcgata gggtaaacaa
    30961 tccatgactt cttgatcact actatcaata atcaaacaaa tccgactgat ttctattaaa
    31021 acctttggag acagccctct tggtaaactc ccagaaacac aaagataatc ccctgcttgt
    31081 aatgctctta tttgatttaa aaaattatga acagctgtct gcggtatttc aggtccttga
    31141 ttcactaatt tgtattcttc tcccgtttga ttgacttgtg taaatacatt gatacgcgtc
    31201 ataccaggca cttcaataaa ctctgtttgg atttgtttct gctgtaaata gtcttctata
    31261 tactttcctg tgaacccagc agaaaaacct aatgccgtac ttgggaaatc tagcatcttt
    31321 aatactagtg acacattcac cccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg
    31381 tttaccataa aaggatgcat ctcatctgtt tcaataaata gatcaattgc caaattcatc
    31441 gtgcatgtgt agatagacat aaccaacgac ctccttgatt ctattatgaa atacgcgaaa
    31501 tagaaaatgt tttcttaatc gtcccaacac aaagaaaacc tttacattta taaactcgtc
    31561 gtgctttttt taactaactt ttggtgaaag aaggaaagaa tatgaatcac aaattaagtg
    31621 aaacagagca gtacctctgg aactttatcg aacacctcat cctagagatc cctaactatt
    31681 ccatcgtgaa actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga
    31741 aaaagaaagg ctatgaaggg tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca
    31801 agaacatcaa tttttcattt ttagataaag tagacaaagg aatccgccga gccattctaa
    31861 aaaatgaaca agaagtggta cgtacaatca atatgttgga aatcgggaac attgaagatg
    31921 caatacaaag aatcaaattt gctgaccggg tttttgtctt tgcccgagga ttttcagaaa
    31981 tgattggcca agaaatgctg gtaaaacttc aattaacggg aaaaaattgt cagatgcata
    32041 ctgatccaga aattatcaaa agcatcagtc ggaaactaac taagaaagac agcgttattt
    32101 ttgtttcttt gaacggtgag aaaacaaata aaaacatcaa aaataagatt tatgtcttta
    32161 tttttcaatt ttttaaat
//
LOCUS       DAYYFU010000028        31843 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000028 DAYYFU010000000
VERSION     DAYYFU010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.028"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(152..2305)
                     /locus_tag="ACHA34_001511"
     CDS             complement(152..2305)
                     /locus_tag="ACHA34_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFM8792157.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2599..3447)
                     /locus_tag="ACHA34_001512"
     CDS             complement(2599..3447)
                     /locus_tag="ACHA34_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8792158.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3671..5611)
                     /gene="thrS"
                     /locus_tag="ACHA34_001513"
     CDS             complement(3671..5611)
                     /gene="thrS"
                     /locus_tag="ACHA34_001513"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFM8792159.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5948..6127)
                     /locus_tag="ACHA34_001514"
     CDS             complement(5948..6127)
                     /locus_tag="ACHA34_001514"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFM8792160.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6174..7997)
                     /locus_tag="ACHA34_001515"
     CDS             complement(6174..7997)
                     /locus_tag="ACHA34_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8792161.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8275..10554
                     /gene="helD"
                     /locus_tag="ACHA34_001516"
     CDS             8275..10554
                     /gene="helD"
                     /locus_tag="ACHA34_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFM8792162.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10647..11294)
                     /locus_tag="ACHA34_001517"
     CDS             complement(10647..11294)
                     /locus_tag="ACHA34_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792163.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11473..12192)
                     /locus_tag="ACHA34_001518"
     CDS             complement(11473..12192)
                     /locus_tag="ACHA34_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8792164.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12323..13474)
                     /locus_tag="ACHA34_001519"
     CDS             complement(12323..13474)
                     /locus_tag="ACHA34_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFM8792165.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13691..14431)
                     /locus_tag="ACHA34_001520"
     CDS             complement(13691..14431)
                     /locus_tag="ACHA34_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8792166.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14435..14758)
                     /gene="rsfS"
                     /locus_tag="ACHA34_001521"
     CDS             complement(14435..14758)
                     /gene="rsfS"
                     /locus_tag="ACHA34_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFM8792167.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14815..15408)
                     /gene="yqeK"
                     /locus_tag="ACHA34_001522"
     CDS             complement(14815..15408)
                     /gene="yqeK"
                     /locus_tag="ACHA34_001522"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFM8792168.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15392..16042)
                     /locus_tag="ACHA34_001523"
     CDS             complement(15392..16042)
                     /locus_tag="ACHA34_001523"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFM8792169.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16052..16363)
                     /gene="yhbY"
                     /locus_tag="ACHA34_001524"
     CDS             complement(16052..16363)
                     /gene="yhbY"
                     /locus_tag="ACHA34_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFM8792170.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16379..17488)
                     /gene="yqeH"
                     /locus_tag="ACHA34_001525"
     CDS             complement(16379..17488)
                     /gene="yqeH"
                     /locus_tag="ACHA34_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFM8792171.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17485..18012)
                     /locus_tag="ACHA34_001526"
     CDS             complement(17485..18012)
                     /locus_tag="ACHA34_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFM8792172.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18192..18977)
                     /locus_tag="ACHA34_001527"
     CDS             complement(18192..18977)
                     /locus_tag="ACHA34_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFM8792173.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18970..19839)
                     /gene="accD"
                     /locus_tag="ACHA34_001528"
     CDS             complement(18970..19839)
                     /gene="accD"
                     /locus_tag="ACHA34_001528"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFM8792174.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19840..21219)
                     /locus_tag="ACHA34_001529"
     CDS             complement(19840..21219)
                     /locus_tag="ACHA34_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFM8792175.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21227..21646)
                     /gene="fabZ"
                     /locus_tag="ACHA34_001530"
     CDS             complement(21227..21646)
                     /gene="fabZ"
                     /locus_tag="ACHA34_001530"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8792176.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21671..22147)
                     /gene="accB"
                     /locus_tag="ACHA34_001531"
     CDS             complement(21671..22147)
                     /gene="accB"
                     /locus_tag="ACHA34_001531"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFM8792177.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22150..23385)
                     /gene="fabF"
                     /locus_tag="ACHA34_001532"
     CDS             complement(22150..23385)
                     /gene="fabF"
                     /locus_tag="ACHA34_001532"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFM8792178.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23404..24141)
                     /gene="fabG"
                     /locus_tag="ACHA34_001533"
     CDS             complement(23404..24141)
                     /gene="fabG"
                     /locus_tag="ACHA34_001533"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFM8792179.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24153..25070)
                     /gene="fabD"
                     /locus_tag="ACHA34_001534"
     CDS             complement(24153..25070)
                     /gene="fabD"
                     /locus_tag="ACHA34_001534"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8792180.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25177..25401)
                     /locus_tag="ACHA34_001535"
     CDS             complement(25177..25401)
                     /locus_tag="ACHA34_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8792181.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25454..26416)
                     /locus_tag="ACHA34_001536"
     CDS             complement(25454..26416)
                     /locus_tag="ACHA34_001536"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFM8792182.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26426..26866)
                     /locus_tag="ACHA34_001537"
     CDS             complement(26426..26866)
                     /locus_tag="ACHA34_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8792183.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27172..28137
                     /locus_tag="ACHA34_001538"
     CDS             27172..28137
                     /locus_tag="ACHA34_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8792184.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28194..28424)
                     /locus_tag="ACHA34_001539"
     CDS             complement(28194..28424)
                     /locus_tag="ACHA34_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFM8792185.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28510..29397)
                     /locus_tag="ACHA34_001540"
     CDS             complement(28510..29397)
                     /locus_tag="ACHA34_001540"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8792186.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29486..30709)
                     /locus_tag="ACHA34_001541"
     CDS             complement(29486..30709)
                     /locus_tag="ACHA34_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8792187.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30726..31772)
                     /locus_tag="ACHA34_001542"
     CDS             complement(30726..31772)
                     /locus_tag="ACHA34_001542"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8792188.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
BASE COUNT         8794 a         6971 c         5488 g        10590 t
ORIGIN      
        1 caatttttgt cacaaccttt tttgccgagt aaacggtgtt caaaagccag tttctcggta
       61 tgaagtcaaa tttaagcaaa aatgtgagaa gctattttca caaaattttt cttcatactc
      121 gaaactaagc ggctttttca cgacctcttt tttattttgc catgaacata tcataatacg
      181 cgtccattat ttctttagca atcgtcaagt ttatgtggtc ttgttcgtct aataagttcg
      241 gcaagacaac actgatggca atttccggat tgtctgttgg tccgtatgcc acgatattac
      301 tattgactgt cgcaatatct ggatgacctt ccgcaaccgt ttctgcagtc cctgtttttg
      361 cggaaaggtc cattttcgca ctggctaatg gtttggctgt tgtgtaagga tcggtaccat
      421 ggacaacttg gtggaatcct tcacggagga tcgccatatt ttctgcagaa atattgaccg
      481 tattttctac tgttgtgcca atttctttct tcacttcacc caagtttcca ttttcgtcat
      541 tgccatagat cgctttgact aggtgcggct gtactcgttt cccgccatta gcgactgttg
      601 aagcatattg agctaattgg attggtgtat atgtgtcaaa ctgtccaaat gccaaatcgg
      661 taaagttacc tggagcatac cattctttgc cattatccgc attgaacttc ttcatataat
      721 caacagaacg tgagatccca ggagattcat tcggcaaatc gatcccagtc gttgtaccaa
      781 gaccaaactc tttgaaagag tctctcaatt gctgataagc tttagcttct tcatctaaac
      841 taggtagtcc cattccaggt gtatagtcta gtcctaacat ctttaacgcc actttgatca
      901 tatacgtatt agaagacaat tccgatgctt tgactgcatc aagagcaact tgtccgcttc
      961 gattaaagac agagcttttt tctgaagcac cttgcagacg aatcggctca tcaatcaaaa
     1021 cttgatttcc gctgatcgcg ttacttgccc agcctgctgt caaagtacca gccttgacta
     1081 cagaccctgg atcaaaggcg ctagtaatcg tgcctaaagc attctcggtg atttctttgg
     1141 aaccttgttc atgactgaaa ccagtcattg ctagcacttc tcctgtctga ggattcatag
     1201 ccactgcata agcaccaggc gaatactgtg ctttgccgtt gttgatcaat gtttgataat
     1261 tccttttcaa aatttcttct acttttgatt ggaattctgc attcatagat aacattaaat
     1321 tagaaccttt ttccccagag aagatttctt tttgattcga aacatttcct tcattatcaa
     1381 gggaaacttc atattgggtt ttggttcctt gcaggacatc ttcatattgt ttttccaaat
     1441 aactttgtcc tacacggtca ttccgagaat aaccttttgc taggtatttc tctgcttcat
     1501 ctgctggaag tccttgcttt tcagtcgtca cactgccaag aatacttttc agtgaacttg
     1561 ctgcattata ttctcgtgtc cagtctgtgc cagtcgaaac tcctggaagt tcagaagcgc
     1621 gttctgccac aacagctaac tcttgatcag taacaccaga gtttttgata tagacagtgt
     1681 ttaattcata tgcactattc atccgtttaa agatggtagc aatttttaat tgctcctcgt
     1741 ttaaattaat ctcatcagct gtgactttat tgaccatcgc actatattgt tcgctgttcc
     1801 ctaattgctt ttcttttgcc gaaagtcttt ttgtggcttc tttcaaatgt ttgggatcag
     1861 ccagccaaaa atctttttta tctctatctg tcagattttc atctaccgga acatcgatca
     1921 gcgagttcag cttcgttgcg atttcaagca aatctttagc cgtaacttgt gttcctctcg
     1981 tatatgtgat ggcctgattc gctttattcg taactaaagc ttttcctgtg gagtcgtaga
     2041 tcattccgcg aggagtactt tcttggaccg taatcgtaga agtagacttc actcgcgcct
     2101 ccatctcttc gccgttcacg atctgcaaat aacccaatct agcaatcaac gcaacaaata
     2161 acgtaaatat aatgaaaaaa agaaaattta agcgaaaagg aacatgggac cggcgaaacg
     2221 ttttgttctt aggttcttgt gcaccttttc ttattttttc taaccaagaa gtatttaata
     2281 tttttttcat aaaatctttc atcatttcct atccttccgc ctatttacat tcatgcttca
     2341 ttgtacccaa aaacttctta ataaaaaaga aaaaatgggc ggagcaagaa attctttacc
     2401 taatttcaag ataaaatcaa ctttttgttt cacagcaatc acttaaagtt acatatccaa
     2461 ctattttttt cattaaaaaa taaacttatt tttgaaaatt atgttttctt aagtcgtgaa
     2521 acactttgat ggatcattta tttgtggaaa actacgtttt gttattctaa aaaacttata
     2581 taaaaaatag taaggttttt atttcgaatc cataaaatat tcttggattg cttctcgtgt
     2641 ttttttcact ttttcgctaa gcttttcgtt tttagccata tagaaataaa aaatgatatc
     2701 gatcgtagca aattgggcca aaagtgaatt agttgccgca cttcgattcg tgatttcttt
     2761 tggtctagat gtttgtacga gtacatcggc tatcttgcta agcgggttat ttccaaattg
     2821 cgtcaacgca atgatttcga tattcatgct tttcgccagc tcggctaatg cgactacatc
     2881 tgcacttcta cctgagtttg aaacaagcca aagcactttc tttttctcat tgctgacaag
     2941 ttgcgggagc aagacgtgga tatctttttc aaaaatgatt ggcttaccga cacgagacca
     3001 tttttgcaga atatcttcta ctactaaaga agaggcgcca accccatata aaaaaacagt
     3061 ctctgccgat tccaaacact ctacgacttt ttggatcgtt tcttcttcta gaatatctac
     3121 tgtttctttt atcgctactt gtgcattgtt accaagtttg tttttgatag agtaaaagga
     3181 ctcatttgct tctatatcac taaaaccttc atcgattcca gtctgtagtt ctgttgaaat
     3241 cgtgattttg aagtccgtca gactttgaaa accgattttt ttggaaaaac gtacaactgt
     3301 cggcgcactc aacccggaag cttctgctaa ttcattagct gtcattgtag ggaccttctc
     3361 aatattaccg ataatgtagt tggccaactt tttttctgct gaagaataat cttccatcat
     3421 actttcaata cgactaatta cgctcataag ttcctccacc cttttaatga tagtctatgt
     3481 aagtatacca tatcagaccc ttattcacgg tcgtatactg gataaaaaac aaaaaaacaa
     3541 tccgcccaac agaatatctt atcccatcaa tcccacagat tctattgagc atctgtgtct
     3601 ttgaggaata cctgttatct attgttcgga ttgctttcta tctactactt ttttacagga
     3661 aatttaccta ttagttttct ctgctgtaat ttttgacttc tgcttccata gcatcaataa
     3721 agatattcaa atcttctaca gctgtttctt tacttccata acgacgtacg ttcactgttg
     3781 catcttccaa ttccttgtct ccaacaacta attggtaagg gatcttttgt gtttgagagg
     3841 cacgtatctt atagcccatt ttttcattac gatcatcaac ttctacacgc aaccccttca
     3901 tttgcaaacg ttctttgatt tcataagcat aatcgccatg tgcatcaaca gaaacaggga
     3961 tgattgttgc ttgtataggt gccaaccatg ttggaaaagc gcctttgtat acttctgtta
     4021 aataagcgac aaaacgttcc attgtagaaa caattccgcg gtggataact actggacgat
     4081 gcgtattttc cccgtcttct ccaacatatg tcaaatcaaa gcgctctggc aataagaagt
     4141 ctaattgtat agttgataat gtttcttctg taccaagagc tgttttgact tgaacatcta
     4201 gttttggtcc gtaaaaggct gcctcgcctt ctgcttcaaa gtaatctagt tctaattcat
     4261 ccatagcggc tttcagcatt gcttgggctt tttcccacat tgcgtcatca tcaaaatatt
     4321 tatctgtatt gtttggatcg cgatagctca agcggaaacg ataatctgta atattgaagt
     4381 cagcatatac tgccaccatc aattcaagcg tacgtttaaa ttcatctttg atttgatcag
     4441 gacgaacaaa tgtatgacca tcattcaacg tcatttctcg tacacgttgt aaacctgaca
     4501 aagcacctga tttttcatag cgatgcatca ttcctaattc agcgatacga attggtaatt
     4561 cacgataaga atggatcgtg tttttgtaga ccatcatgtg atgagggcag ttcattgggc
     4621 gaagaacaag catttcgcca tctcccatat ccattggcgg gaacatgtct tcttgataat
     4681 gatcccagtg tccagatgtt ttgtacaact caacatctcc cattattggt gtatatacat
     4741 gttggtaacc tagactaact tctttgtcta cgatataacg ttcgatcgtg cgtcggattg
     4801 ttgccccttt tggaagccag aatggcaaac cagaacctac ttctggggaa accatgaaca
     4861 agtcaagttc ttttcctagt ttacgatggt ctctttcttt tgcttcttca cggagacgga
     4921 tatattcttt caaagctttc ttgtcaaaaa aggctgtccc gtatacacgt tgcatcattt
     4981 gattgtttga gtttccacgc caataagcac ctgccactga taataattta aatacttgta
     5041 tacggccagt ggatggaaca tgtggtccac gacacaaatc gatgaattcg ccttgttgat
     5101 aagcagtaat cacttcttct tctggcaatt cactaatcag ttctactttg tacggatcac
     5161 ttgcaaaaat ttctaatgct tcctgttttg aaagaacacg gcgttcgatc ggcagatttt
     5221 ctttgacgat tttcatcatt tctgcttcga ttgccggcaa atcttctgca gttacttggt
     5281 tttgtccatt atctgtatcg tagtaaaacc ctgaatcgat agctggtcca acgccaaagt
     5341 ggatatttgg atagaggcga cgcatggctt gggccatcaa atgagctgct gaatgacgga
     5401 ctaagccaag tgcatcttca tggtctggtg tgatgatctc aatacttgca tcttcctcga
     5461 ttggacgagt caaatcaacg agttttccgt tcactttacc agctaaagct ttttttgcta
     5521 agctttttga aatgctttca gcaattgcca atgttgttgt tccagactca aattctttga
     5581 ctgcaccatc tggaaaagta atttttatca taatagtttc ctccttgaaa tgttttgatt
     5641 gatcagatca tcaagctgaa aaacaaaaaa atcctagcat ctcttgaaaa gagatactag
     5701 gacgaaaagt tcgtggttcc acctaatgtt caaacgcaaa tgcgttcctc aagcgttgta
     5761 acggtccgac cgcccttgct tcaacaaggg tttcgaaagt ggtcatcctt tggttttctc
     5821 ctggaaaagt ttcagcctag cttttcctct cttatcgaag actctcaaat tcagttggct
     5881 ttctcatcaa tcaattatgt gtttatttaa ccactaataa atagaaaaat caagtgggaa
     5941 actaaactta ttttttccat tccccattta caccgtatgt tccttttttc atctcattga
     6001 tgatgacatg gatgtgttct tttggtgcct gcgtgttttt atgaacagct tccgtgatat
     6061 ctttcatcat attttctaat tgttccggac tgcgaccttc tactaattcg acatgtacga
     6121 atggcataat gctctctcct ctttctgatc gttctttttt caactcgatt ctatcatact
     6181 atgtttgtct tgggtactga atcaggaaaa atggctggat ctttttttgc tctcaaggca
     6241 ctcaaaatag agaccgtatc cacgacttct tgcaaaacag ctccgaataa tgcggggatt
     6301 agtcccgtac ttgcaattaa catcaaaaaa atacaaataa agatgccaat cagtacagac
     6361 tgtttcgcta ttctcatcgt atctcttgaa atcactactg cttttgctac acgcaagaga
     6421 tcatctttca agatgaccgc atctgcactt tcactagcag ctgtagaacc atgagccccc
     6481 attgcaatac cgatatctgc agcagcaagt gctggtgcgt cattcacgcc gtctcctacc
     6541 atcgcaacag gtctttgatc tttagggata ctttctaaaa tggtcagttt atcttgaggc
     6601 aagctacggg catgtacaga atctaattgg actttttctg caatcgtttg agcaatggca
     6661 gatccatccc cggtgatcat catcaaattt ttcacatcaa gttttcttag ctccttgatt
     6721 gtctttgctg cttctgggcg aatctgatcc gaaaatagga tcgttcctgc aaaagatcga
     6781 tcaattgtta catagattgc tgtattggac gtttctattt cttcagctga aacaaaagag
     6841 gcacgtccaa tctttacttc atgattattg actactgctt ggattccttg accggtcact
     6901 tctttcagtt gactaacagg atacagttgg ctgtttttca cgttgttcac taacgaacga
     6961 gctaagatat ggctagattc ctgttctgca ctagctgcat acagaagtag ctcttctttt
     7021 tttattccat tgactggctg aatcgcctct acttccaata tccctttggt aagcgttcct
     7081 gttttatcaa aagcaatcgt ttttgtacgt gcgagctttt caatcgttgt tccagttttt
     7141 acaacgatcc cgtttttact tgaacggctc attcctgcaa ctaaagcaat gggcgcagct
     7201 agaatcagtg gacaaggtga tgccactacc agtacctcag caaatcgtaa agggtctttt
     7261 gagaaccacc aagaaatacc accgatcaaa taagcaatca acgtgaaagg gactgcataa
     7321 cgatcagcta aacggacaaa tcgggcaggc ttttcttttg attcttcgac tagtttaata
     7381 atcatttgat actggctgtc tgcggcacgt ttttcagcga tcatttggat cgatccgtct
     7441 ccatttaaag accctgacat aatctgatct ccaggtttct tggtttccgg gcgggactca
     7501 cctgtcaaag aagattcatc gaccgaagat tctcctttgg tcactcgacc atctgcgggt
     7561 acaagttcgc caggcttgac tagtaactgg tcatcaatat ttatttcatc gattactata
     7621 tcctgcaatt ttccttcttt gaatcgatgg gcaattcttg gggtgttatc taacaaagac
     7681 tgcagttctt tacccgctcg tttattcgcg taatcctcta gactatctcc gcccgtaagc
     7741 atgatcaata tcatcaaact agcccagtat tctttgacgg ctaatgttgc aaggattgcg
     7801 gtaatcgcta atacatctac gccatatctt cctgaacgta acgtcttgat catttccaaa
     7861 aacatcaaga aggcgagaat ccctccagta aatgcgacaa tcgcaaatgc aagggccggc
     7921 tggtgaaaaa caaattcact taataaagct atcccaccaa taagcaaagt aagtaaaaat
     7981 tttttgaaat gtgtcattgt tttttctcct cttttatgtg acttagagag gttagtaata
     8041 aatttccagc tgcctctttc atcttaagca tagtccttga ctagcacttt ttaaaacaat
     8101 aggccttaat tgaaaacaac tatcactatc agcgtttcca aaattcatca gttctacaaa
     8161 caaaaatatt cagcaaggat tactttttcg attcatcagt taaaaagtac cttttttcga
     8221 aaaactatgc tatactttta attcgttatt tacggcggaa ggagcaaaca tgcgatgaat
     8281 gagaaacaac tagaacaaac gcacgtggat gaaacgatcg aactaatcca gatcgaacaa
     8341 aatatgctgg atacaaaaca acaagaattg actcaggaaa tgaatcattc gacaaaagac
     8401 acagctaatc acaagattcg agctggttcc aatgaagctt tttatgaatc tgctgttgaa
     8461 tatcgccaac atgagcagga attgcttttg aaataccata ctcttgaagc acagcaaaaa
     8521 cgcctgcaaa cgttgtctgt catgaaagat agtccttatt ttgcccgtat cgactttaaa
     8581 gaagaccgtg aaacagaaac attatatcta ggtatcgctt ctttaagaga tctggcagaa
     8641 gaaacaatcg ttattgactg gcgcgcacca attgccaatc tttattatga aggtgagatt
     8701 ggtcccgcat tttatgaaac tgaaacagat agaatagatg tcgatctgca gttgaaacga
     8761 caatttaaaa tccaagaagg acgtatttta tctatggtag atacgtcgga agtaatcaat
     8821 gatgactttt tgctagagat attggatgaa tcatcaagca accatatgaa aaacatcgtc
     8881 tctacgatac aaaaagcaca aaatgcaatc attcgggaca caacaagtaa agtaatgctg
     8941 attgaaggaa tcgccggaag tggaaagaca tccgctttgc ttcaacgtat tgcttttctg
     9001 ttgtatcata atcgcaagtg gctcgaggct gaaaacgttc ttctattttc acccaaccat
     9061 ttgttttctg attacatctc gactgtattg ccttcattag gtgaaagcgg cgtgcctaca
     9121 cagacattca aaaattattt ggtgcagctg ctgccagagt tttctttact agatgaacag
     9181 caacaagaaa gtggtttttt gctaggggaa aaagatccga tacaagtgat gaaaagcgga
     9241 ttgaccttag tcgatcagat tgatcgctat atccaaggca tcacaaacta cggtcccttg
     9301 ttccgtgata tgaaaataaa tggccgtacg atcctatcaa aggaatcgat tcgccaatgg
     9361 tataaagaaa caaatgaaca attaccttta catcatagac tttctctatt acaaacgaag
     9421 ctcctgaaaa aattaggcgg tctgcaaaaa gacgaaacac gtcagcaatg ggtcaaagat
     9481 ctggcggaag aacagctcca agaactatac gcaacagatc caaatcttga atatacagaa
     9541 caaaaggagc gttcattacg taaaaaactg gccaaccaaa tcgtacgtaa aagattccgt
     9601 cgaatccatc gagggatcat gaattatcaa tttgtcaata taacgaaaca atatctccac
     9661 tttctacaaa ctgtgccact ttctcttcta acacgacata aaatcagcga gaccaggtgg
     9721 caggaacatt tgacaactgt tcgtacacac ttgcgtcaaa gagaattgcg tcaagaggat
     9781 gctgtactat tctttttact gatgcgccga acatatccag tgacggttac acacaaagct
     9841 cgctatatct ttattgatga aatgcaggat ttcgcaccat cacaagtggc attgctgcaa
     9901 gccttgttcc caaaagcaaa tttgactttc tgtggagatc taaatcaaaa tgtctttgga
     9961 aatgaaacga tcgttggatc attggaagac cttttcgtgg accgtgaagt cactcgtttc
    10021 caattaacga caagctatcg ctccacaaaa gaaatcactg attttgctaa tcattttatt
    10081 gtgaaagaac atcaagtaga aacaacagca agaaaaggag accttccttt gctcatccaa
    10141 ggaacaacag atgatatcaa attgaattgg ttagcagata ccattcgaac gactcaggga
    10201 aaagcccggt attggcgtac agcaatcata gctaaaacac aagaagaatg cgaagctctt
    10261 tatgctcaac tgccagacga gttgaaacaa caagtccagc tgattttaaa tgagagtgac
    10321 tttatgaagc gttccattat tttacttcca gcctttttag caaaaggtct ggagtttgac
    10381 cgagttttct tgtgggatgt agatgcagaa catttttctt ctcagcaaga caaattgatc
    10441 ctttacacca tgtgtacacg tgcgatgcat gaattgaccc tttttacctc caataatgaa
    10501 agtcctttgt taaacaatat ggatatttca tcttatgaga aaaaagtact gtaagtaaaa
    10561 agacaactgc tatcattaat gttagcagtt gtctttttat ttacaataat aattgcagaa
    10621 aaagtaattc aatcatttaa ggtcatttat tctatatata aatccaaatc tgtttcagga
    10681 tattttaatt tgacaagcga aataatcatt ttcagttctt ccatgttttc tggatattct
    10741 gggtcaaata ccttgctatg acggccaatc ccgccattgt cgatcaatcg gtaaattcga
    10801 ttccattcaa tgaattggtc aattgattgg aaattgctga ttggatagcc gccacaatcc
    10861 aaaatctctg atacacgaaa tgctaggtat gcactatcga tcactttatt ttttttcgtg
    10921 tctatcttct catttctttc gataagaatt ttttcaggac gatgtttcaa gctccactct
    10981 aatagtagaa agaaagcctc catccgatac tcttcttcaa ataaccgaaa ggctgaatca
    11041 gaacgacgaa aaagcagcgg aatcagataa ttttgtctat aaatcagcat tggtgcgcgg
    11101 tccaccatac actctaaata acggttcaga taccctgtac cgacatagtt tcctaatcga
    11161 tagccttcaa cgccgaatct tgcgtatttt tgcgctaatt ttgaacaatc agttacgttc
    11221 acagctaact cttctacttt gcggtaaccg cttaagatac gttcaaaagg ttctaaagtg
    11281 atttttgctt ccatattctt ccccgtccat cttccacata cttcatatca tttcacatga
    11341 tacttatgct ctcatcatac cacaggttga attagaaaag aaaataaatc acacaaaaaa
    11401 gcaagacaat ctgttagtga gattgtcttg cttttcgaaa aagactgaat acttttcgtt
    11461 aaaactggtt gcttagtctt ctaatgccac attatggaac acatgctgta catcttctag
    11521 atcttccagt gcatcaatca ttttttcaaa tgtttcttga tcttctggag atagtgttac
    11581 ctcgttttga gggatcattt ggatctcagc gattgaaaaa tcagtgattc ctttttcttt
    11641 taatgcttct tgtatactat ggaaatccgc tacttcaccg taaacgatga tttgtccttc
    11701 ttcttccatt acatcgcgaa catcgatgtc tttttccatt aggtattcaa ggatctcgtc
    11761 agcgtcttct cctgcaaaag caaagattgc tgtattgtca aacatgtaag aaaccgctcc
    11821 ggaaacaccc atgttcccgc catttttccc aaatgctgcc cgtacgtctg ctgctgttcg
    11881 attcacattg ttcgtcaatg tatcaacaat caccattgag ccgcttggtc caaatccttc
    11941 atagcgcaat tcagagtatg tttcatctcc tgcacctttt gctttttcga ttgctcggtc
    12001 aataatatgt tttggtacat tataagtttt agcgcgatca atgacgaaac gtagtttttg
    12061 gtttgcatgc ggatcaggat cacctgattt tgctgccgcg tagatttcaa tcccaaattt
    12121 tgcataaatc cggctattat tagcatcttt agcagttttc tttgctacga tatttgccca
    12181 tttacgtccc atattttcac ttcgctttct gatttgatag tttcataacg caaaaacgtc
    12241 ctgttccatt atacttgtga atcacgattc tgtgaagctt tttttctttc ttccttcatt
    12301 cttctcattt ttagtcaatt atctatactc ggattgggat ttgcccgaaa ttctgttccg
    12361 gaatattgcc tcccaattga tagatttgat cacttctaac taacagttcc gcttcttgtg
    12421 cctctttttt tccgattcgg gaataaatag aaatctcttt tttttctttt aagaatcttc
    12481 ttccagcatc ggtaaatcca agtagtcgaa gataattttt attttgttgg acaggaatct
    12541 cttgcttttt gatattcagc aagacataac aagataatcg ttggatccgt gtccatgtat
    12601 atctttttgt ttttaccttt gacacaaaat cctcaaaact atcagcagtt gcagctgctg
    12661 ttttcatgcg cgattctatt ccttcggtca tctgatagat atctgtcagt tccagcaaag
    12721 tagaagataa caaccgataa cgcaaatacg gccaataaga ttcccaagtc tgcagcgaac
    12781 aagttgaaag atcttcttct gtctgaggcg gcacaagtcc agctattctt tttccttctt
    12841 ctaacgcatg gcgaatggct gttgcactgg ctatcctctg atctgtgatg attttttcat
    12901 gatagcctgc acttttacgt cggatcggat gtaattccat agggtggaca tacttggcat
    12961 tttcctttgc ataacttaat cccaagatat gatttggcga agaaaaatca agcgtaatat
    13021 cgggatacag actgcgaaaa acctcagtca ttttttgcgg ataactatac gtttgatcag
    13081 acagttcttg aaatgcctct tctatcctat cttgattctc ttggacaaag cgtccgaaac
    13141 tgctgtaatc aaacgaagtc tcttcttctg ttccaaaaca caaactgtca cagtccagtg
    13201 tttgcaagat ttttactgca cctttagcaa aatagtcagc tgactgaacc gcccattcga
    13261 cgggaagctc cactactaaa tccacaccat tttgtaaagc ttcattcgcg cgatgccatt
    13321 tatcgatgat agccggttcg cctctttgca aaaaattccc gctcattaca gcaatgacga
    13381 cctctgctcc tgttattttt cgtgccattt ccacatgata gcgatgtcca ttgtgaaaag
    13441 gattgtactc tacgataatc ccgcagcttt tcatgcgcat cctccttcat agttatcgat
    13501 aaaaaagaaa tagagacaag taaaaaaagg caaaaatcgg aaaaaatacc cgaactatat
    13561 aaactattct cgcaaacaca atacttctgc gacgaaaagc tgcactgcaa gagcaatgcc
    13621 tatatcgttc ggatatataa tttatgttga agatttattc ttttcttgtt aactcgagca
    13681 ggcaaaaccg ctagtctttg tatgctacaa agaaccagcg ctttgatttc tctgatgctg
    13741 cttcgtctgt aaaatcagaa aatgcttcga ctttcgaaaa gccgctgttt tccagcattc
    13801 ttaaataact ttccaatgga taggtacgct cttgatgtag ttcatcttca cgaataaatc
    13861 gaccagaccc ttcttgttct tcaacgaaaa aggtaagaaa atgttcgatg ctgtgtttct
    13921 cgcttcctgg ataactatcc cacaaaaaag caaaattatc cccttgataa tgatagctat
    13981 attctggaaa aacctcatct acttgatagg tggagtgtac gtcaaataaa aatcggccat
    14041 tttcttctaa cgcttgatag acttcatcaa acacttgctg tacctcttgt ctatttttca
    14101 tatagcacag cgaatcagaa aaacaggtaa ttgcctgata ttgtccgatt tctgataaat
    14161 cgagcatatt gccttccaca aattggatcg atgcgtcttc ttcatatgct cgctggctgg
    14221 caatcatcaa catctcttct gaaaggtcta accctgtcac tttatatcca tcttttgcaa
    14281 aggcaaccgc taatgctcca gtaccgcatg ccagttccaa aatatttttt gtgtcttctt
    14341 gtaaatgacg tttcgaaaaa gctaaccatt gctgataaag tgtttcgtcc atcacttcat
    14401 cgtaaatgaa agcaaatgtt tcataagcca tatgttaatc caaccattca ctaagatcga
    14461 ctagcggagc atctgaccat agtttttcca gattatagaa atttcgttct ggcgcatgga
    14521 aaacatgtac gatcacatct ccaagatcga tcaataccca ttttccgcct tcttttcctt
    14581 caatacgttt tacttcatat ttgttttctt cttctttatc aatgatttct tctgtaattg
    14641 cgttgatttg acgttcgcta tttgctgaac agatcatgaa ataatctgcc aacaaggaaa
    14701 catttcgaac gtctaaagcg ataatgtctt ctgcccgttt tgaatctgct gcttcaatcg
    14761 caatttttaa ttgttcttta ctatctatga tgatttcctc ctaatatgag cagcttattt
    14821 ttttgctacc cagtaattat atgtttcgat tgtttttgga taaatctgtt gttcttgcgc
    14881 aagcaaatgg gccaatgtat gttttgtttc aaatgctaca gcttcgtcta gatcagccca
    14941 tgcaatggct cgtgcatctt gtaccccagg aaaatctcga cctggttcaa tatagtctgc
    15001 tacataaatg attttgtcaa ggagactcat cttagcagcc ccagtcgtat gcacccggat
    15061 tgcatgaaga atctctgcat cggtgattcc taattcccgc tctacaaacg aagcgccaac
    15121 aagaccatgc cagattgcat tattataatt caataactcc ggatcaaaac cgtctcttac
    15181 gattaccatt ttgaactcct catctggtcg ttcttttgca taatcatgtg tcaatgcagc
    15241 aatactagct ttttcaggag aagcaccgta ttttttggca agagccacgg ctgtttcttc
    15301 tactccaagc acatgcttga agcgttgttc gctcattctc atctgtattt tttgcatcag
    15361 gttttctcta gaataagcag tatattcatt actatagtcc atattcatac aacccttcat
    15421 tttgaatata atcaattact ttatctggaa caaggtagcg gatagaacag ccttctttga
    15481 tcttttgacg tattttcgtg gaactgatgt cgatctcagg tacatctacc caaattacag
    15541 gatatggagt atccgttgtg tatcctggtc gacgtatacc cacaaagttg accatagaag
    15601 tcagctcatc gattttatac cattttggca aatattctac catatcccca ccgataataa
    15661 agtaataatc tgtatccgga ttgttttgcg tcaattcctt catagtatca taggtgtaac
    15721 ttttcccacc acgagctaat tcaatcgttt caatctgcaa aaaaggattg tcttccaccg
    15781 ctaattccaa catgttgagt cgatgttttg catcaatcgt ctgtttctca tcaacatgag
    15841 gtggctgata agaaggcatc aaaaatacgc ggtcaagacc taaattccgg ccagcttgtt
    15901 cagccatcac taaatgtgca aggtgaacgg gattgaatgt tccgcccaag atccctactt
    15961 gctttttctt ctctaagaat tgaggcattt cttcaggaaa taattgtgaa cgaacaaatg
    16021 tcttagcttg cgtattcatc atggtcactc cttatattgc ttttacttct tttgatagcc
    16081 tttgatactt ttcttttgtg gaaggtttgt ataatacaat aacacgtcca atgatttgga
    16141 cgatttcgca atctatttct ttttctaata cttgagctgc ttcttctgca atctcatctg
    16201 tattttgaag taacgatact ttgatcagtt ctcttttttc taaagcttct tcaatttgaa
    16261 cgatcattgc tgaattgatc ccaccttttc cgatttgaaa aatcggctga agatgatgcg
    16321 ctttgctacg taaaaaacgc ttttgttttc ctcttaaact cataattttc cttcttcctt
    16381 aaatcaatgc tttgcgtttc aatacatcta caccctttgg cgcccagccc gcaattacta
    16441 ctggcgctgt tacagtgatc cagcctagac cggcaaaaac gatgtctgtt ttttctttca
    16501 cagaaaactc aaaacgaaca agctccggaa atgcctcgac ttcatcagga cgaggaggct
    16561 gtaataatcc acctacatgt ttttgataaa attcgtctgc tttttccagt ttcgttcgat
    16621 gaatatttac atcatttgac acataagcaa taaatgaagc acgattgccg ctgataaaat
    16681 caaatcttgc aagtccgccc aagaaaagtg tttgcccttc attcagctga taaactttag
    16741 gtttgatttc ttttaacggt gcaatgatcc gcaaatcttt tttacctaag tagtgcgcca
    16801 tttgatggcg atggataatt cccggtgtat caatcaaaaa atgcccgtca tctaaaggaa
    16861 tctcgatttt atccaaggtc gttcctggaa actgcgaagt tgtgattaca tcttgtactc
    16921 ccgctgtatt tttgatgatt tgattgataa gcgtcgattt tccgacattt gttactccga
    16981 cgatatagac atcccgtcca tcgcggtatt cttcgatttt ttctaatagt tccgtcatct
    17041 catgagattt cttagcacta gttaataata catctactgg acgaattcct gcttcgtaag
    17101 cacgttcctt catccattgt gttaatttag tttttttcaa tgatttaggt aatatatcca
    17161 ctttgttccc aactaaaagt actggattgt caccgacaaa acggtgtagt cctgggatta
    17221 gtgaaccatt gaagtcaaaa atatccacca cattgacgat caatgcatct ttttgcccta
    17281 attcattcag taagcgcaaa aagtcgtcat ccgtcaatga gacatcttgg gtctcattat
    17341 aatggcgtaa tctgaaacaa cgctgacaat agacttcccc ggaagctaaa cctttttcca
    17401 aagccgtttg cggcgtgtat cctaattcac cgggtttgtt tgtttgtata acggcaccac
    17461 agccgataca ttgtaattct tctgtcattc gattccacct cgccagccca tatcagggtg
    17521 ttttgctaat agataggcca taattctttt ttcccaaaaa cgattaaatt gtgtcttcca
    17581 actatctgtt gcgataatgg gttttacaag gatgctacgt atccctgcac gattggctcc
    17641 acgtatatca gtcataatct gatcgcctac cattactatc tcatcatctg ctaaatccaa
    17701 ttgtttcttt gctatgttga tccctcttgc taaaggtttc attgctcgtg caacatacaa
    17761 aagatcaaat ttttcaatcg ctcgcgcgac acgttttgaa ttattgtttg aaacaacaac
    17821 aacagggatt cctgcatttt tcatttctaa aatccaagta agcaactctt ctgttccatc
    17881 tggattattc catgcaatca acgtattgtc taaatcggtc aatactgctt tgatgcctag
    17941 attctttaat tgggcaggtg taatctgata gatggcttct accatccatg ttggtttgta
    18001 atttgaaaac atgctgtctc ctcatctgca aaaaatgaag gagcacataa agtactcctc
    18061 aaccccgtat tatactgcat atcagcaaaa aaaactacct ctatactcgt tcgtctttca
    18121 gaaaaagcat ttctttacaa aatgaccaaa aataaagatt gatcacagaa aaatttagaa
    18181 acaaaaaaca actaatattg gcggaacctc ttgtatcgtt tttccagtaa tttatgctga
    18241 tctaatcgaa caagttccgt caatctttca atcaacgctt ttttcataat gtgtgcgact
    18301 ttatatcgtg gcaactgctc gccgttgaac gtttcaggaa taactcgatc aacgattcct
    18361 aactcgcgta gttctggcgc agtaattttc atcaactcag ctgcttctgc ggcgcgacta
    18421 ccgtctttcc acaaaatcga cgcaaatcct tctggagaaa gcacagcata aatcgtttct
    18481 tccagcatcc agacttcatc cgctaaacct agagcaaggg caccgccact tccaccttca
    18541 ccaatgatga tggcgatgat cggtaccttt aaatcagaca tttcgagcag atttctggca
    18601 atagcctctc cttcgccgcg ctcttctgct cctacaccgc aatatgcacc tgctgtattg
    18661 atcaaggtaa tcaccggtct attgaacttt tccgcttgtt tcatcagtcg taaagctttt
    18721 cgatagcctt caggatgggg agaaccaaaa tttctgcgga tattttcctg cagatcccgt
    18781 cctttttgaa taccgatcac tgttaccggt ttgccatcta atcgagcgat tccaccaaca
    18841 atcgcttcgt catctgcaaa taaacgatca ccatggcatt cctcgaaaga atcgaagatc
    18901 tcttcaaaaa aatccaagct tgtccatcgt tctttagctc ttgctaactg aacgacatca
    18961 tgtgctgtct tatccacgat ctatccatcc tttctgtgta tgcaattcaa gaagcgtatg
    19021 gattttctgt ttcaattcca tgcggggaac aatctgatcg acaaaaccat gagataataa
    19081 gaattcagcc ttttggaaat cttctggaag ttcctgcttg atcgtctgtt cgatgacgcg
    19141 tcttccagca aaaccgatca agctttgggg ttctgctaga ataatatcgc cttccattgc
    19201 aaaactggca gttactcctc cggtcgtcgg gtccgttaaa acagttagat aaaaaagacc
    19261 ttcattgcta tgccgtttga cagcagcaga gatttttgcc atctgcatta gagaaaagat
    19321 tccttcttgc atccgtgcgc cacctgaagc tgtgaacaag acgactggaa gactttcctt
    19381 tgttgctcgt tcgaataatc tggtgatctt ttctccgaca atcgttccca tacttcccat
    19441 gataaaatta ggatccatca ctccaataat gaagggaatc ccctgaatgg ttgctttacc
    19501 agtgaggact gcttcatgaa gtcctgtttt atcttgcatt tttttaattt tttccgtata
    19561 ttcgggaaat gctagcggat ctttggttac aagatccgta tcccattctt caaaactttt
    19621 ttcatcaata atcaaagcta gacgctgcca tgcaccgatt cgaaaagcgt atccgcaatg
    19681 agggcatact ttttcttcac cgatgtcttt cgtataaagg atatgtttgc aatttggaca
    19741 ctttgcccac atgttatcag gaacagatgg cgagttagca ggtttgctct ctctattagg
    19801 attgatccga atatatttct ttttcttaaa cagagccatc tatgcgcctc ctatctccgt
    19861 ttctggtgtc caattcggta aaaattcttc ttgtaaaaat gctgtgctgt aatcacctgc
    19921 tatgactgca ggatgactaa tcaaatccat ctgaaattct gcgttcgtga tgattccttc
    19981 tgtgataaat tcacttaagg ctcgctgcat cttcattaat gcttcaaaac gcgtatttcc
    20041 atggacgatt atttttgcaa tcattgaatc ataatatggc gggatcgtat atcccgggta
    20101 aactgcactg tctactcgca gtcccatccc acctgctgga agcagtaagt tttggatctt
    20161 ccctggagaa ggagcaaaat ggaaagcagg attttctgca ttaatccggc attcgattgc
    20221 gtgtccttgg aatactactt cttcttgaca cacattcaaa ggctcaccgg ctgctatctc
    20281 cacctgtttt tttactaaat cgattcctgt gaccatttct gttactggat gttccacttg
    20341 gattcgagta ttcatttcca tgaaataaaa atcgccttct tcatccatca gaaactcgat
    20401 cgttccggca ttttcataat tgactgcttt agctgctcga acagcagcgt ctcctagtgc
    20461 ttctcttttt gtttgtgaga tcactacaga aggagattct tccagtactt tttgattgtt
    20521 gcgttgtaaa gaacaatctc gctcgcctaa atgaatgaca tgaccaaatc gatcacctaa
    20581 gatctgaact tctatatggc gagcaggata aatgattttt tccaaataca tatcatcatt
    20641 tccaaatgcc gctgctgctt cttgctgagc tgaagaaaaa tgctgtggga gctcttcttt
    20701 tgattgaact ttccgaatgc ctttcccgcc accacctgca gctgctttca gcatcactgg
    20761 ataaccaatt ctgtctgcta tttccaatgc ttcttctact gtagataata caccagtact
    20821 tcctggaatg actgggacat ttgcttcttg catcaattga cgcgcattga tcttatttcc
    20881 cattgcatcg atcgttgcac ttttaggacc aataaatgtg atattgcact cttcacacat
    20941 ggaggcaaat cggctatttt cagataaaaa accaaatcct ggatgaattg cttcggcttt
    21001 tgtcacgatt gcagcgctca gtacttcctg aacattcaga tatgaatcaa cggcttttgc
    21061 cgggccaatg cagattgctt catctgctaa ctgtgtatga agtgcttcgg catctgcttc
    21121 tgaatatatc gctacggttt ggatccctag ttcgcggcat gcacgaatga tacgtacagc
    21181 gatttcaccg cggttagcta tcaatacttt agaaaacata tttcacctat ccaatcataa
    21241 aggttaattc cgcttcggct acttttttac cgtctacttt agcgattcct ttgcctattc
    21301 cggctgaagc tttcactttc aatatctcaa cttcaaggta taaggtatct cccggtgtaa
    21361 ctttcttacg gaattttgct ttgtccaatc cgccgaaata agccgtcttt cccttgaatt
    21421 gttcaagaga aagtaatgca actgctcctg cttgagccat tgcttcaaca atcaataccc
    21481 ctggcataac aggttcttct ggaaaatgtc cttgaaaaaa gggctcattg atcgttacat
    21541 ttttcttagc aacgatccgt tctccttcaa ccatttcttc cactcgatca atcaaaagca
    21601 ttggataacg atgtggaatg atttctttta tttcttgtat gttcattgtt ttccctcttt
    21661 cctgctttgc ttatttttgc tgaatcctaa agagtggctg tccatattcg acaactgact
    21721 cgttttctac aaggatttct gtgatcactc cgtcggcctc actagttatc tcattcatca
    21781 gtttcatcgc ttcaataatg cagataactt ctcctttggc aacttgatca cctacttttt
    21841 tgaaagctgg tttgtcgggc gctggttgca gataaacgat tcccacgatt ggcgaaacaa
    21901 cctcgattcc atcaaatact gccgattctt tttctttcat tatttctgga gcagttgttg
    21961 cttgttcagg tatgacattg actggctccg aaactggcaa cgtttcgttt gtttgaacca
    22021 tattcatgcc tcgacttgtt ttatttttat tcatatacaa ttcaaattga ccttcacgca
    22081 aatcgaattc cgttagcgag gattgatcaa attgactgac taattctctg atttcattga
    22141 tatccaaaat tattcctccc agcgtttcat gcacagaacc gcattatgtc cgccaaaccc
    22201 taatgaatta ctcattgcat aagtaaaact tgcaggtttg ctttcgtttt tgacaactgt
    22261 caagtcaatc tcaggatctt gtttttctac attgatcgta ggtgggataa attgatgttc
    22321 taatgccagc aaagttgcca aggcttcgat cccgccggct gctcccaata aatgtccagt
    22381 catcgatttc gtgctgctga cgtaagtatc tctgaaattt tcacctaatg cgatttggat
    22441 tgcttttgct tctgcctggt cattggctgg tgtacttgtt ccgtgagcat tgatatatcc
    22501 gacttgttcc ggctgtacac cagcttcttc catcgcaagc ttcattgctt tagatgcacc
    22561 agaaccatct ggagtaggag ctgtcatatg gtaagcgtca cagtttgcac cgtatccaac
    22621 gatttctcca tagattttgg caccgcgagc ttgtgcatgt tctaaagatt caagaactag
    22681 aacgcctgct ccttcaccca tcacaaaacc attccgattt tcgtcaaacg gaatcgaacc
    22741 acgattagga tcagttgatt ctgagagtgc tgttaaagct gcaaatccag caatccccat
    22801 ctcacaaatc gttgcttctg ttcctccagc aagaatgaca tcagaatagc catgtttgat
    22861 gttacggaat gcttccccaa ttgaattcgt tccgctagca caagctgtta cgatagctgt
    22921 acaaatacct tttgcaccta cacgcaaagc aatatttcca gctgccatgt tggcaatcgc
    22981 cattggtacg aacattggag aaactcgttt tgcacctttt tcatgtagac gaataacttg
    23041 gttttcgatt gttggaagac caccgatccc agatccgatg atgacgccaa accggtcttg
    23101 attcattttt tctttatcta aaccgctcat ctccattgct tctaaagcag cgtagattcc
    23161 atagatcgaa aacatatcca ttcgtttgct atctttttta ataaaatatt tatcgtaagg
    23221 gaaatctttg acttcacccg ctaaggtaat ccctgtttct gaagcatcaa atttagtgat
    23281 cggtgcgatg ccattcgtcc cctcttttaa gctattcaaa aagctgtctg cgtcatttcc
    23341 aataggtgaa gtgactccat agccagtaat taccactcga ttcatttgtt tgctctcctt
    23401 ttcttatccg tgcatgacca atccgccatc gacattgatg acttgtccgg tgatatatgg
    23461 attttgtgct aagaaaactg ctgtttgtgc aatatcttct acttgtccga aacgctgcat
    23521 cggaatttgc ttttccgctt gttccttgac tttgtctgct aaaacttccg tcatatcagt
    23581 tgtgatgaat cctggcgcaa tggcattaca tgtaatgcct ctagttgcag cttctcgtgc
    23641 cacagatttt gttaatccta ctacgccagc tttactagct gcataatttg cttgaccaat
    23701 atttccaatc aacccagata cactagagag attaatgatg gccccttctc gctgcttcat
    23761 catttttttc aatacgtgct gcgtcatatt gaaggttcca atcaaattga tatccagaca
    23821 ttttttgaaa tcttctgcat ccattcgcat cacaagtttg tcatttgtga ttccggcatt
    23881 gtttactaac acatggatcg atccaagctt ttcttctgct tctttgatca tttgtcccgc
    23941 tttctcataa tcagagatgt caccggaaac tcctacgcat tttacaccga atgcttcgat
    24001 ttcttctatc ttttcttttg gaatctctcc gcgaccattc aaaatgatat ttgctccagc
    24061 ttttgcaaaa gctaaggcca tcgctttgcc gattcctctt gtactacctg tgataaatac
    24121 attttttcct tttacatcca taacgttcct ccttatagta ttgctattgt ttcatcaaat
    24181 gtctgcttat tctcaacacg cagaactggt attgttttat cgattttttt cataaatccg
    24241 cttaatactt ttccggggcc gacttcgatg acttgttcga tgccgattgt ttttaactta
    24301 tggatactct catagaaacg aacaggttgc atgacttgct gttcaagtag tcccgcgatt
    24361 gtttctttct tcattatttc cgttgttgtg ttgcttatga taggaaaaga aggttcagag
    24421 aattgaatct gacttaggtc cttcgccagt ttttttgctg ccggttctaa aatagctgta
    24481 tgaaaaggcc cgctgacatt tagagggatc attcttttga accctttttc ttttaatagt
    24541 aaaacagctt cgtctactgc tttttcttcg ccaccaatca cgatctgctg aggtgtatta
    24601 taatttgcag gagatacgat gccgtacttg ctagcttcat gacagctttc ctcgatcgtt
    24661 tcaatcggcg cattcatcac tgcaaccatt ttcccgcttc cagctggggc agcttctgtc
    24721 atataagcgc ctctttttgc tactaacgca actgcttcag tgaaggatag cgcattacta
    24781 gctactaatg ccgaatattc tcctaaactc agtcctaaag ctgcatctgg gatgattcca
    24841 tgctccttca ataagcgata atacgcgatg ctgactgtta gaatagctgg ctgtgtatat
    24901 tgcgtctgat ccaaacgttc attttctgta aagcaaagtt cagccatatc gtagccgaga
    24961 atctcacttg cttcatcaaa tgtctgcttt acaacggctt cttcatataa atcttttccc
    25021 ataccttgat attgtgcacc ttggccacta aataagaatg ctgttttcat gtttttctcc
    25081 aatatatcca gaatatatgt ttgattcttc ctattcaaaa aggccgagac attcctattt
    25141 caggaaatgt cccagccccc aacctaattt taatgattat gcagtttgtt tttctacgta
    25201 agtcactaaa tcttgaaccg ttttgattcc gtcttctgtt tcgattttca cgtcaaattc
    25261 atcttcaatt tcattgatga tttggaataa atccaagctg tcagcttcta agtcttcttg
    25321 gatgtcagta gtcaatttga tttcttcagt ttcttttcct aattcttctg cgatgatttc
    25381 ttgaattttt tcgaatacca taataatata ttcctccgat attgtttgct ttttattttt
    25441 ataatttaac agtctacaac tccatgagaa tacttcccca tgttaaacct ccgccatagc
    25501 ctgtaaatat tactttctgc tgttttccta aggtgatctt tccactagaa actgcttcat
    25561 ccaataagat agggatgctt gcagcagatg tattcccata tttatccata ttggtcagga
    25621 acttttcttg cggcatcttt acttttcggg caattttctc aataattctg aggtttgcct
    25681 gatgcaaaag cagataatca actggtttgt ctttcatgac ttcttggata ttgtttgcaa
    25741 cgtctcgtac ggcaaaatca aaaatatctc tgccagtcat ttgcaaataa tacgaacctt
    25801 ctgaatcttc tgaatagaac ggatttttat tttgctggta gccagcggtc aatgagctac
    25861 ttctggtacc gtcagctgct aatttttctt tcagcaattt tttctgtgga ttcgcttcta
    25921 agagaatccc acctgctcca tcgccaaaca acacagcagt cgtacgatca gaccaatcga
    25981 ttagcttaga tagtgtttca ccaccaatca ccagtccctt agttgagccg cagcggatca
    26041 ttttttcagc catgcttaac gcataaacaa atcctgagca cgcagcactg atatcaaaag
    26101 cgatagcatg agttgctccc aatttccctt gtacttgtgc tgctacagat ggtgtcgtgt
    26161 aatcgggcga cattgttgct actaaaataa aatccaaatc tttagggttt agatttttct
    26221 gttttatcaa tttttccgcc actaaaatgc ataaatcaga agtctcctga tttgttacac
    26281 atcttcgttc acgaataccc gtccttgacg aaatccattc atcagaggta tccatcatct
    26341 tggaaagatc attattagta ataatctttt caggaaggac gctggctgtt gcagtaatca
    26401 ttccaaattg ttccattaaa tatcctcact tatagtcttg caagaagtcg tgcaagtttt
    26461 tcagtgcatc cagcaaggca tgctgttcct ccgtagccat tccgtctaag atgttcttga
    26521 ccatttcacg atgaaaatgc tgatggaccc gaaatagtaa ctttcctttt ttcgttagtc
    26581 ccaactttac aacgcggcgg tcatcttcac tgcggatacg ttcgacatag ccttttttta
    26641 ctaacttgtt aatagcagtg gtcaaggtcc caacagtaat tgatagttct ttggctactt
    26701 ctgaagttgt ttttttcttg tacatcccga ttgcctcgat cgtgtgcatc tctgtgattg
    26761 acagatcttt gaattgagat tttttcagct cagactcttc gattgtcaaa atatcattga
    26821 agacactaac aagataatca tttactgttt ttaaatctgg ttccatgttc caccgtccac
    26881 taattagttt gattatcaaa tcttttgatg ttcaaactat atcttaactt ttttcaaaat
    26941 gcaaccttaa aagatcattt ttttactata tgagcttttt ttaagatttt actcatttaa
    27001 aacaactgat aaatcagcat ttataatttc ttttattaga aaaatcatct atctcacata
    27061 ttttgattat caaagtattt tctgtgaaca gaactataga attccctaaa acttgtccat
    27121 tatttcaaaa tttgttatag tctttttatt caaagcgtaa aaggagatcc tatgttaaaa
    27181 gaaaaaatga aaactctccc catgctgccg ggtgtctatc ttatgaaaga ccgagatggt
    27241 aaggtcatct atgttggtaa agcaaaacgg ctcaaaaata gagtaagcag ctattttcat
    27301 cagaataaac agcattcaaa aaaagtatta cgtatgattc atcatataac agattttgat
    27361 ttcgtcgtag tagacacgga attagatgct ttattgctcg agtgtcaatt gatccaacat
    27421 taccgtcctt tttataaccg acaaatgaat tatttcagta attataatta tgtacatatt
    27481 accaataagg gatttgtatt aacagatact cctacggctc gaacatacgg gccttttcgt
    27541 ctctataaaa aaatgccgtc tattttgcgt ataatggaag aaacttatca aatgccttgg
    27601 ttgtctgaaa ttagtttgtt agctttgcga gtccagcttc ctgacctaca ggaaatgact
    27661 tttgaacaga aaaaaaagga actccaaggg ctgtttcaag gccgaaataa aaagctactg
    27721 acttacttga aaaaaagaca acaacatttc atctatcagt tgaatttcga aaaagccggt
    27781 atgcttcaaa aagatatcga attagttact tattttatta gacggatcca ggaacaaaag
    27841 cagtttttaa gaacaccatc gctcaccttc agcatgccat tagcagcaga tgagagtcag
    27901 aaaaagcact acctcgtttg ttatggtcaa cttgcagaaa caatgattac ctctggtgac
    27961 gtttctcctg acttctatta tgaaaagaaa gaagctcatt tatctttgaa acgtcaattg
    28021 tcaaaagaag aaatcgatcc tgtacaaatc ttgatcagtt accgcaaaaa attagaaaaa
    28081 gaacagtttg aaattgaact gttaaacaaa aaagaagctg aaaagcaact aaattagttg
    28141 cttctcggct tcttttaggc atcttcaact tcaaatgtca attatcctaa aatttattgg
    28201 attaagtcat agatttccat cgctacgata tcgatattat caaattcgta ttgctgatga
    28261 gtatctgttt cagttaattc aaatgtatcg gtcgctttat cataagttac tgtacacttc
    28321 tctacgcctt ctttttcaaa acggcgaact tgcagttcat tatctgctga ctcgaccata
    28381 gcctccagtc gttttatgat tgcgactaat tgtgaatgtg tcatcggagg tcctcctttt
    28441 cttatttctg ttatattgta tcaaaagcat agcctacttg cattgatttt ctctaaaaaa
    28501 actcgttttt tatgattttt tttctggtat ttttccttca ggccaccaaa gtttgatcaa
    28561 agcttgggtt ccgtcattct caaaaccttt atctgccaac tcttcatata gtttcaatgc
    28621 ttgggtagtt gctggcaata caagatccat tttttctgct tcttctaatg caatctttaa
    28681 atctttgata aaatgtttga caaaaaaacc aggtgtgtaa tcttctttta aaatacgagg
    28741 agaataattg cttaaagacc agttagctgc acttccacct gaaagcgtct caatcacttt
    28801 ctttaaatca agtccgtttt tttgtgcata gacaagcatt tccgtcattc ctgtcatcgt
    28861 acctgcaatc atgatctgat tagccatttt tgtatgctga cctttcccag cagaaccatg
    28921 caatgtaaat gtcgtaccaa attctttgaa taaaggcagt acttgatcat aaacggcttc
    28981 ttctccaccg accatgatcg tcagtgtacc attctttgct cctaagtcac caccggatac
    29041 cggtgcatct aacgcatctg cgcctttttc ttttgctgtt tttgcaattt tttctgccag
    29101 gcttggtgta cttgtagtca aatcaactaa aatatgtcct gagatatctg cagaaaaaat
    29161 ccccgactgt ccatagtaga tttcttctac atctttgggg taacctacca ttgtaaagag
    29221 aatattactt tgttctgcta ccgcttttgg tgtatctgcc cagatagctc cttctgttac
    29281 taaaggatca gcttttgctt ttgtccgatt atagacagct acttcgtgtc ctgcttctag
    29341 tagatggcgt gcgacagcac ttcccatgac gcctgttccg ataaaaccaa gtttcatttt
    29401 ttcgacctcc taaaaatcaa aaatccttgc ttctatgcta gtatacgcta gaaacaagga
    29461 ttttttcata aaaaatgttt gaattttatc ttttaaatga atttaatttt attttgattc
    29521 gttcaatcga gtcttcttct tcttttcttc gcatctcttc ttcatgataa acaaccatgt
    29581 cttgataaaa ttcgatcata gcttcgccga aatgtgtaga tgaaatatca taaagtttag
    29641 cttctaaaac ttttggatct ttaggaactt cactggaaat ataatcaata agtgctggtg
    29701 caaagtcttc gtcggtttca aaagttttcc ctaatgaagg atgatcgaac aatcggttca
    29761 aataagcatt tccttccacg acacaaggtg ttccggctgc catcgcttct gtataagtaa
    29821 gcccttgtgt ttctgaagta gaagcactga caaaataatc tgccgcttta taataaagtg
    29881 ctacatcatt gttgtccact tctcctgtga actgcacaaa ttcttctaac tgcagttctg
    29941 agatcaattc ctctaactct tctacataag gtccttttcc tacgataact aatctggcgt
    30001 taggataagc ggagatgacc tgaggaaacc cattaatcaa ggcttggata tttttctcat
    30061 aagaaatcct gcttaaagaa agaatcatca aatggttttc ctgaagacct agtgactccc
    30121 ttaatttgct gatatcctgc tgagtgatat cagaacgttt gaatttttca atgtcgatac
    30181 cagtaggaat cacgcgaagc ggtgctatta caccatagct gcgtaatgta tcaataacac
    30241 gttcacttgg acaaactaca cctgtcgtat gatttgcaaa taatctagac aagtatttta
    30301 catgggattg cctgatcact tttcctttac cgatataatg aaggtagtct tcatacatcg
    30361 tatggtaagt atggatcaga ggtattttca acttcttagc taccatcttc ccaagtatcc
    30421 ctgctccgaa ttctgtatgg gtatgaataa gatctagttc tagttctttg gcaaccaaat
    30481 aagcatacca catgcctcta acaacaactc ttcgatcctt gaaagaaata aatggtacac
    30541 ttggcattcg aatcacatct ttttccaact tatccgcatt aggatcggtc gttgtaaaaa
    30601 tatagacttc atgcccatgt ctttctaatt cttgttttaa tgttttgatc gatgtagcaa
    30661 ctccgctgac ttgcggaaag taggtatcgg taaaaaagcc gattttcatg tttttcgcct
    30721 ccaaactagt ccttgacctg atttactttt tttaatttgc tagagacttt ttcattcagt
    30781 acactttcgt atacattttt aagttcttca ccgattttat tgatacttcg ctcttttgct
    30841 gtttgataac cggcttgtgt cgtactcggt agttcttgat tggtaatctg ttggatcagt
    30901 cggataaatt cttcgtttgt cttccccata tagcagttct tgctatcttc taaccagcca
    30961 ttatacactg gaatatctcg aacaagtacg ttttgtttgc ttgctaaagc ttctaaaaca
    31021 acgatccctt ctgtctcttc ataagaaggg aaaaagaata gatctgcacc tgaatacgca
    31081 ccttctatga tctctccacg aatgtaacca ggaaacagga cattttcagg atgatcttct
    31141 tttacaattt tccggatgtt ccgaggaatc gagtacatgg gaacatgacc aaaccaaata
    31201 aatgtgtatt cgggcatctt cttagcaatt tctacgaaat caatgattcc tttacgttcg
    31261 aaaaacaaac cgacacaaat gattactttt tgatccggag acagtttgaa atattctctg
    31321 aacttttgtt ctttcattgg atcagggtga tatttctcta agtcgatccc attggaaata
    31381 gcttgaatcg gcacacggat accatagctt tccaacaact gttttgaata aggtgttggc
    31441 gtgatcagat agtccgcttt ttggtacaat cctaccagat atttttttac gataggagaa
    31501 agctggttcg atccgataaa ggaatttcgg aaatcttcct ctgtagaatg cgcatgatag
    31561 acaacttttt ttccttctct tctggcttta ttgaccatat tatgactatt gattccgtat
    31621 gtattgatgt gcaggatgtc ataatcttct ttttcatcta gtgtataaga gataccgact
    31681 tccgtcagag cacgtttttg atgatcaagc gcacgcccga ttccagattt tgcaagcatc
    31741 ttttcacttt caaaatataa taataccttc accttacgtc ccccttcact tatatagtat
    31801 aacatagcct acaaactgtc actcaaactt aagccctatt ttt
//
LOCUS       DAYYFU010000029        28306 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000029 DAYYFU010000000
VERSION     DAYYFU010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.029"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..81
                     /locus_tag="ACHA34_001543"
     tRNA            8..81
                     /locus_tag="ACHA34_001543"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="ACHA34_001544"
     tRNA            101..173
                     /locus_tag="ACHA34_001544"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="ACHA34_001545"
     tRNA            201..274
                     /locus_tag="ACHA34_001545"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="ACHA34_001546"
     tRNA            288..361
                     /locus_tag="ACHA34_001546"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="ACHA34_001547"
     tRNA            385..474
                     /locus_tag="ACHA34_001547"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="ACHA34_001548"
     tRNA            487..560
                     /locus_tag="ACHA34_001548"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="ACHA34_001549"
     tRNA            579..654
                     /locus_tag="ACHA34_001549"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="ACHA34_001550"
     tRNA            656..726
                     /locus_tag="ACHA34_001550"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="ACHA34_001551"
     tRNA            740..813
                     /locus_tag="ACHA34_001551"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="ACHA34_001552"
     tRNA            838..925
                     /locus_tag="ACHA34_001552"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="ACHA34_001553"
     tRNA            947..1018
                     /locus_tag="ACHA34_001553"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="ACHA34_001554"
     tRNA            1041..1130
                     /locus_tag="ACHA34_001554"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="ACHA34_001555"
     tRNA            1143..1216
                     /locus_tag="ACHA34_001555"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="ACHA34_001556"
     tRNA            1235..1307
                     /locus_tag="ACHA34_001556"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="ACHA34_001557"
     tRNA            1311..1383
                     /locus_tag="ACHA34_001557"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="ACHA34_001558"
     tRNA            1388..1458
                     /locus_tag="ACHA34_001558"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="ACHA34_001559"
     tRNA            1471..1544
                     /locus_tag="ACHA34_001559"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="ACHA34_001560"
     tRNA            1556..1627
                     /locus_tag="ACHA34_001560"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     regulatory      1816..1895
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            1893..3086
                     /gene="metK"
                     /locus_tag="ACHA34_001561"
     CDS             1893..3086
                     /gene="metK"
                     /locus_tag="ACHA34_001561"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFM8792189.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="ACHA34_001562"
     CDS             3216..4688
                     /locus_tag="ACHA34_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8792190.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="ACHA34_001563"
     CDS             4771..5559
                     /locus_tag="ACHA34_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8792191.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="ACHA34_001564"
     CDS             complement(5626..6294)
                     /locus_tag="ACHA34_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFM8792192.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="ACHA34_001565"
     CDS             6409..8121
                     /locus_tag="ACHA34_001565"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8792193.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9893
                     /locus_tag="ACHA34_001566"
     CDS             8124..9893
                     /locus_tag="ACHA34_001566"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8792194.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10098..10664)
                     /locus_tag="ACHA34_001567"
     CDS             complement(10098..10664)
                     /locus_tag="ACHA34_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8792195.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(10664..11620)
                     /locus_tag="ACHA34_001568"
     CDS             complement(10664..11620)
                     /locus_tag="ACHA34_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFM8792196.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(11620..12276)
                     /locus_tag="ACHA34_001569"
     CDS             complement(11620..12276)
                     /locus_tag="ACHA34_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8792197.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            12646..14664
                     /locus_tag="ACHA34_001570"
     CDS             12646..14664
                     /locus_tag="ACHA34_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8792198.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    14734..14940
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15091..17505
                     /gene="leuS"
                     /locus_tag="ACHA34_001571"
     CDS             15091..17505
                     /gene="leuS"
                     /locus_tag="ACHA34_001571"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFM8792199.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            17853..19406
                     /locus_tag="ACHA34_001572"
     CDS             17853..19406
                     /locus_tag="ACHA34_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFM8792200.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(19787..21571)
                     /locus_tag="ACHA34_001573"
     CDS             complement(19787..21571)
                     /locus_tag="ACHA34_001573"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8792201.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(21586..23307)
                     /locus_tag="ACHA34_001574"
     CDS             complement(21586..23307)
                     /locus_tag="ACHA34_001574"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8792202.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            23407..24168
                     /locus_tag="ACHA34_001575"
     CDS             23407..24168
                     /locus_tag="ACHA34_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8792203.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            25135..25403
                     /locus_tag="ACHA34_001576"
                     /pseudo
     CDS             25135..25403
                     /locus_tag="ACHA34_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            26075..>28306
                     /locus_tag="ACHA34_001577"
     CDS             26075..>28306
                     /locus_tag="ACHA34_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8792204.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         8925 a         5059 c         5989 g         8333 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa taaagaggtt
     9901 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag cttctcatgt
     9961 ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt gaataaagag
    10021 gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac atatcattta
    10081 tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg cggctggttc
    10141 ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag tttggaacaa
    10201 aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta gacgacaagg
    10261 atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt gcgtgtagtc
    10321 tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt gaaaatccct
    10381 gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc ttgtagaaac
    10441 aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc atcgattgct
    10501 tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc tgctagaaaa
    10561 accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg ttgcaagatt
    10621 tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg ctcacctttt
    10681 tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca tcaattgcat
    10741 tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc cacggcgcat
    10801 cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg tcgcaaatga
    10861 cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag tcccgcaaca
    10921 ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata tctgaatcta
    10981 aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt aatccgttga
    11041 tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca tctaaataag
    11101 tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt gtttgcaatc
    11161 caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca actacttcat
    11221 ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt aaattgacta
    11281 cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta aaattttgga
    11341 aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg atttctttct
    11401 ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa ccagatacac
    11461 tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag tcaaatcctc
    11521 catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg taattccaag
    11581 aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct tctttctgta
    11641 agagttatct atttgttttc ttttttctct atacactgga taagtcaggc acagccaaga
    11701 aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca gataaatgcg
    11761 gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg tgcgtaaggt
    11821 tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa tcatactgcc
    11881 tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg ccaatgaagg
    11941 acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa ttacaataaa
    12001 tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc ataaagccgc
    12061 acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa agagttggta
    12121 gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat cgaaaccaga
    12181 aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga caagaaagaa
    12241 gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt gaagcaatga
    12301 cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac caatgtatga
    12361 ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga aagacatttt
    12421 ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa tccttttaaa
    12481 caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt acggtaaatt
    12541 acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat attaagagaa
    12601 gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga aaacattgca
    12661 agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa acgaggggca
    12721 gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt acagccggtt
    12781 cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac agcggcattt
    12841 atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct atatgcatca
    12901 gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt atctcaagca
    12961 ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt ctacatggat
    13021 acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg acaatatcct
    13081 tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc atttggcaac
    13141 ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga tgcgacagct
    13201 tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa taatatcatc
    13261 gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg cggtggagcg
    13321 actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag caattctggt
    13381 aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg tgattctgtt
    13441 tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg gaacaacatc
    13501 caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca ggaaaatggc
    13561 tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg tacatcaaac
    13621 ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt ttggaagatc
    13681 tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat caagaacaat
    13741 ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa tgctggagca
    13801 aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc aagttcttct
    13861 aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg gagcgtctca
    13921 aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca aaataacttt
    13981 atctacccag gacaacaact gactgtttca aacagtggat caacaaacag cacgtcaggt
    14041 aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg cagcagttcg
    14101 tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc aaataaaaac
    14161 ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt catttatcca
    14221 ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg ttcaactact
    14281 tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc atccactggt
    14341 aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa caaacatcat
    14401 atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat ctatccagga
    14461 cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc agttactggt
    14521 tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga cagtcatgga
    14581 attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat ttatccaggt
    14641 caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt gaattccaca
    14701 aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg aagtagtaaa
    14761 aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc ttttgaactc
    14821 gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg gccgttacaa
    14881 cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta cgtcctataa
    14941 aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag aaaacaaagc
    15001 aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat aatagaaaca
    15061 agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga aattgagaaa
    15121 aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga tcctgaaaag
    15181 ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct acatgtaggg
    15241 catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag tcaaggatac
    15301 aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca atatgcgcta
    15361 gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt ccgacgccaa
    15421 atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac tgatccggag
    15481 tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct agcttatgaa
    15541 gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa tgaagaagtg
    15601 atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat gcgccaatgg
    15661 atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt agttgattgg
    15721 ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg agcaaatatt
    15781 gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg accagatact
    15841 ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag agaaattacg
    15901 acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa aaaatcagat
    15961 ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc ttatgcaatc
    16021 aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt atcttcatac
    16081 ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga attcgctcaa
    16141 actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa agctgcttac
    16201 acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa acaagaagca
    16261 atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga agtcagctat
    16321 cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat cccaatcatc
    16381 cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt acggttgcct
    16441 aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa tatcgaggag
    16501 tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac gaatacaatg
    16561 ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca taacaaaaaa
    16621 gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta cattggtgga
    16681 gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt cttatacgat
    16741 atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg aatgatctta
    16801 ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc tgatgatgtg
    16861 gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg gccattagat
    16921 gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt ggatcgtgta
    16981 tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac attgaatgat
    17041 ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga ctatgaaaat
    17101 cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc ctataaagtg
    17161 gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc gattgctcct
    17221 cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc ttacgcacca
    17281 tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt tttccaagta
    17341 aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga tgagctagaa
    17401 aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac agtcagaaaa
    17461 gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc tctttgtcaa
    17521 taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca ttctgtctca
    17581 gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga ttttcatgtt
    17641 ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct ctgtaatcat
    17701 caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga aattgagagt
    17761 acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga ttttgacgca
    17821 taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt attatcgaag
    17881 tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa agaataatgt
    17941 tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct gtatcgttcc
    18001 tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa ttaaataatt
    18061 tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt gtttttcttc
    18121 tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg cttagtcaaa
    18181 gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat ctttattttg
    18241 agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag ttattgctga
    18301 gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc ggaatgaaca
    18361 cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag tcttcgcctg
    18421 gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc attcttttag
    18481 ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat ataaatgctg
    18541 cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca aatgaagggt
    18601 tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat gatctcaaac
    18661 ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat tgggtatttg
    18721 tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg gggctgattc
    18781 ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca tggtttatct
    18841 attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa acatggcaaa
    18901 cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta ttttatggca
    18961 acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact ttgtacattt
    19021 acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg atcaatcagc
    19081 cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca cgtgaaagtt
    19141 tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg attccggaaa
    19201 actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact caaataagcg
    19261 cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat gcagggaaaa
    19321 tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc gttcagcaac
    19381 tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg ttcagcaccg
    19441 agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt gtaactcgtg
    19501 attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc gtattttaca
    19561 agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt aaaaagggat
    19621 tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc caaaaagtat
    19681 cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa aagctatcga
    19741 tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac gctatctgaa
    19801 attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca gaatgtgttc
    19861 ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt tcaatcgttg
    19921 acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc attgcttgct
    19981 gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa ataacgacag
    20041 gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct tgagataacg
    20101 tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga ataaagtgat
    20161 cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc attttcccgt
    20221 aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa accatcccaa
    20281 aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg aattcgatct
    20341 ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt tttcctgcac
    20401 cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg acttgattca
    20461 tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt tttcctttcg
    20521 gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca tctaatatct
    20581 caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca taagaagcgg
    20641 tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag aagccgatcg
    20701 tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg aaggcgagtt
    20761 gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca cgtttaaaag
    20821 cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct tgctgattga
    20881 atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt aaaacaccca
    20941 gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta gttgtaacaa
    21001 ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg atataaaaca
    21061 gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg atacctgtca
    21121 ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg tcacctactt
    21181 gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt tcttttcgca
    21241 ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata aatgaagtga
    21301 tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat gcttgtttga
    21361 tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag agatcatcga
    21421 tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat aaactgcata
    21481 gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa cgtatcaaag
    21541 cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata ttccttgtga
    21601 ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag taccttgtcc
    21661 aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg tactgaggcg
    21721 ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg ctcttaatgc
    21781 tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga tctctgctgg
    21841 tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat aattagaacc
    21901 gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt cttctaattg
    21961 agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc ccatctgcaa
    22021 gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac tgatgtggga
    22081 gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta cttttcctat
    22141 cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag atccggtccc
    22201 gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt ctagaactgg
    22261 atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat ttgtctgatc
    22321 aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct ctgcttgcag
    22381 tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca ataccatagc
    22441 tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag tcacgacacc
    22501 gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag aagcataaac
    22561 gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt tcactttgat
    22621 catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat atgattgatt
    22681 atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga tggtgatccg
    22741 atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca gtcgataaac
    22801 aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata gagaataacg
    22861 aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca tttggcaaaa
    22921 taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca aaagagatcc
    22981 agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga aatatttctc
    23041 ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag aggcaactgc
    23101 agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt ttataactga
    23161 ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg gtacacctaa
    23221 tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc ctttgttttt
    23281 ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat acttttcgat
    23341 tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa aaagaggtga
    23401 gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga tttgccaaaa
    23461 tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca agaagagaat
    23521 gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt tttttataga
    23581 aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt gacggagtta
    23641 tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt gatgaaacaa
    23701 aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga tacaaacgag
    23761 gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga gtttgaagat
    23821 attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat gagtatgcca
    23881 gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt acatttattt
    23941 actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt cttattgaaa
    24001 tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag aatgtatgaa
    24061 aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac ggatgaaaac
    24121 aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga aatatgtgtt
    24181 taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct ggttcgatta
    24241 cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt tttagaggaa
    24301 atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg taattgtcct
    24361 cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat ggtgaactga
    24421 gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat gagaatgata
    24481 aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac attattacaa
    24541 tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt aatcaaatag
    24601 tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt tttgcttata
    24661 aaataattat aagtatttag agatttttct tctaacattc ttcaaacaag aaaaatcaca
    24721 aaggttattg ttatttttat atattctttt taagaatgat tgttttttta tagaaatttt
    24781 aaaaaataaa acacgttatt gatggatagt tagtttgtta tttggtaata ttatagaagt
    24841 tgactggtag tgatttattt ttcgtcgttt tataaaagtg ttattttttg gaaaaattca
    24901 caaatatgta acgaatgttt ttctggaatt tgctgtgtta aaaagcagta tgactaatat
    24961 aaatactatt gtcatttatg caatttttta tggatagtaa ttttagaggg gaatcaatga
    25021 aggaagaata gaagggaaaa atagttgcaa atacgccagt tagcaaaaat agcaatacat
    25081 cttcaagctt taaaattcga gagaatcatc ttggtagtgg aatcaactac aacaaaagga
    25141 ttaaaccaat taatagatag aattaccaat ggaaaaatag aaaaggtagt tattctatac
    25201 aaaagttgtt taattcgctt cggttatgaa cttagtgaaa ccctttgcaa tatatggtac
    25261 agaatataga aattattgac attacagaaa atgatgaaga tttaatacag attgtcacta
    25321 tttgtagata ccttttacaa ggaaaccgag ccaataaagc taagaagatg attaaggagt
    25381 tgatggaaga cgatacaagt taagaaagtg aaactacgcc caaataaaga gcaaaaagag
    25441 taattggacg tggaaaatgc aagaaatgaa ccatcgattc ggtggacagt ttattagtaa
    25501 tgacggtttg agaacatcca tgacgagaat gaaggaacat cctaaatgcg tttagttgaa
    25561 ccaagtgcca aataatatgg ccaaataagc gatcagttat cgatgaatcc caagtattct
    25621 aaactatata ttcattttga tgctaagcat tgatatattt ttttgggatt gatgaaggtt
    25681 aaccaataca gggattcact gatgaaattc tagagtctga ccttggtatc aacgatttag
    25741 tgattgtttc taatggaaaa gtataaaagt atataagtac attaacaaaa taactattag
    25801 ggttacgctc cagcttgtgc aaggcactgg caggaggaat cgtgaaatca taattatggt
    25861 taagccacac ggacaactaa aagtcataca aagctatcga atcaatcaac agaagacaat
    25921 cggcaatcat tagctttgtt tacagcttac attgcaacgc ttgagttgaa ccgaaaatac
    25981 gagggaggta acgcagccat tttgagttgg ggaatctttt tagaaaatta tcgggtacct
    26041 tgttgtttag tcaaaattat aagggggagt aatagtgagg aacaaatttc ataaactgtt
    26101 aagtatgttt ctagttatgc ttattgtgat caatcaattt gggaacataa cggttctagc
    26161 tgagtcttta gcagaacagc cagagcgttc gctgacgatc gaagttgatc aagaggaggc
    26221 tgctttgggt gagcagttcc agttgcgagt cagaggaaca ccagacctgc taaatgcatt
    26281 ggtcattgat aagagtgagg atttgattga aggggaagtg aagcaactca atgattacga
    26341 acgtctgatt acttattctt cccagcattc aggtaaatac gaattgaccg caagaagcgg
    26401 tgatattcaa tcaaatacgg tctcaataga aatcagtgta ccagaagaaa gcacaagtga
    26461 gtcttttaat gtaccagaag aaagcacaag tgaatctttt aatataccag aagaaagctt
    26521 aagtgaatct tttaatgaag ccagatcatc tgagccatct ataacgcagg aatctcagat
    26581 tgaaagacaa gaggagctct tgaattatga catcggcatc tctgctgagc gccccacttt
    26641 gccgagcagg catacacaaa cgttaaaggg atacattcaa ggaggcgctg gcgatattct
    26701 tgaagaatat ggggagatgg tcgtacgagt tcctagagca tcgattggct taattgagtt
    26761 cattagagtg gaggtcgctg atgattcacc tttaaagcca ctggcatttt accaagaagg
    26821 cacagacgtt gtttttccat ttacctttga ttatgaagga ttaaatgaag ccgctcagtc
    26881 gtatacattc agtatcacgt ttgaagttgc tttctttcaa tcaggcggta ctgagccaaa
    26941 taatgacttt caagtgagtc ttacttatgc tgagcgagca gcaaacacgg aattttcaat
    27001 tacgcctatt ccttcatcaa gacccatttt ttcgaaatta tactatggct ctagagaaat
    27061 cgacagccaa ggaaatcttg ggaatctgct gattgacggg caagacgtga ccaacagtaa
    27121 taaatttatt ttgcaggtaa attatgaagg atcacgaaaa gacggtgtag tggttaccga
    27181 tgtactaccg caaggaacaa ctttggcacc gaatccgact acatggagtt ccactggaaa
    27241 tagagaacct atttcaggtg gggttcggat cgcagaagta acgaatccag gtgaagcaaa
    27301 cgttagaact cattatgttt ccgaggaatt tgtggacaaa gtatctttta ataaggaaac
    27361 gaatacactt gttgtggact ttggtaatct agcagagggt caatcgtatt ttattgaata
    27421 tgctcttgaa gtgaccgatg tcgacttgtt caatgaagga agacaagagg atgggttttc
    27481 tcggaatcga gcgagtatga cctacaataa tcaggtaatg agccgagact accgcttgag
    27541 aatccctgaa agtaatcttc cggcaatctc tttaagaaaa agtgtggaca aaaatgttat
    27601 caatctcaat gaaaatgaac taacgtatac tctgcatgtc tcgatgtttt ctggaacaag
    27661 aaatacgctt gttattgaag atgttttgga agaaggcatg gaatttgtgt catttctatc
    27721 agatattccc tctttcattg atgccgaagt caataatgga atagttcgtt tcagtgcgaa
    27781 taatacgatt ggtggtccga caagttttac cctctctttt aaggtcaatg tagaggcgta
    27841 tgcggtcggc gatcaaattt taaactttgc aacgcttgtg aatagtgaca atcgattgaa
    27901 tacgagtact gtaagaacaa ggaaaatcga tgggcgtatt aaagtaatta aaactgatga
    27961 gaatggccaa aggctggcag gagcaaaatt tgaaatttta tcaagcgatt cacaagtgat
    28021 acaagaaggt ataacggcag aaaacggaga atttttaagt gaaccgcttc ctgtaggtag
    28081 ctatcaagtc cgagaaacag aagcaccgga agggtatctt ttagatgcga cggtacatga
    28141 tgtttttata aatgaatact ccatagaaga agtgacgtta accattgaaa acatccttga
    28201 tacgggtggt gttgaactaa ttaaacatgc tgaaggcagt ggtgaagtcc ttcaaggagc
    28261 tgtttttgag ttacagaacc gagaaggaga aacgctacaa accggt
//
LOCUS       DAYYFU010000030        27634 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000030 DAYYFU010000000
VERSION     DAYYFU010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.030"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACHA34_001578"
     CDS             476..1339
                     /locus_tag="ACHA34_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8792205.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACHA34_001579"
     CDS             complement(1468..3090)
                     /locus_tag="ACHA34_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8792206.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACHA34_001580"
     CDS             3339..3860
                     /locus_tag="ACHA34_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8792207.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHA34_001581"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHA34_001581"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFM8792208.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACHA34_001582"
     CDS             5941..6759
                     /locus_tag="ACHA34_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFM8792209.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACHA34_001583"
     CDS             complement(6862..7059)
                     /locus_tag="ACHA34_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792210.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACHA34_001584"
     CDS             7228..7806
                     /locus_tag="ACHA34_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8792211.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACHA34_001585"
     CDS             7901..8038
                     /locus_tag="ACHA34_001585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792212.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8851..9354)
                     /locus_tag="ACHA34_001586"
     CDS             complement(8851..9354)
                     /locus_tag="ACHA34_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8792213.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="ACHA34_001587"
     CDS             9713..10738
                     /locus_tag="ACHA34_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8792214.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11061..11939
                     /locus_tag="ACHA34_001588"
     CDS             11061..11939
                     /locus_tag="ACHA34_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFM8792215.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12415..12531
                     /locus_tag="ACHA34_001589"
     CDS             12415..12531
                     /locus_tag="ACHA34_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8792216.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13459..13797
                     /locus_tag="ACHA34_001590"
     CDS             13459..13797
                     /locus_tag="ACHA34_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792217.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13891..14121
                     /locus_tag="ACHA34_001591"
     CDS             13891..14121
                     /locus_tag="ACHA34_001591"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFM8792218.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14521..14892
                     /locus_tag="ACHA34_001592"
     CDS             14521..14892
                     /locus_tag="ACHA34_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFM8792219.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(15166..15858)
                     /locus_tag="ACHA34_001593"
     CDS             complement(15166..15858)
                     /locus_tag="ACHA34_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8792220.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15861..16646)
                     /locus_tag="ACHA34_001594"
     CDS             complement(15861..16646)
                     /locus_tag="ACHA34_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8792221.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16883..18283
                     /gene="lacG"
                     /locus_tag="ACHA34_001595"
     CDS             16883..18283
                     /gene="lacG"
                     /locus_tag="ACHA34_001595"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM8792222.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18308..18625
                     /locus_tag="ACHA34_001596"
     CDS             18308..18625
                     /locus_tag="ACHA34_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8792223.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18638..20311
                     /locus_tag="ACHA34_001597"
     CDS             18638..20311
                     /locus_tag="ACHA34_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFM8792224.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20325..21956
                     /locus_tag="ACHA34_001598"
     CDS             20325..21956
                     /locus_tag="ACHA34_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFM8792225.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22828..24018
                     /locus_tag="ACHA34_001599"
     CDS             22828..24018
                     /locus_tag="ACHA34_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HFM8792226.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            24152..26341
                     /locus_tag="ACHA34_001600"
     CDS             24152..26341
                     /locus_tag="ACHA34_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8792227.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            26338..27573
                     /locus_tag="ACHA34_001601"
                     /pseudo
     CDS             26338..27573
                     /locus_tag="ACHA34_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
BASE COUNT         8865 a         4575 c         5450 g         8744 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacgctt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctttac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
    10861 ctttcaaaaa aattttatat ttattctatc aaaaaatttt gtttatgatg gtaactgaat
    10921 aaactttcct gagatggtca atgctttcct tgaaagtctt acagatattc tttcttcata
    10981 gaaagtttaa gttttatagt tagtgtacaa taaaagtatg tttataccat atggtgtccg
    11041 gataatcagt catttaaaca atgaggttca aagatatgaa aaggggaaat ttgatgaaga
    11101 aaataagcaa tgaaatccaa attctggcag aaactttaac tcttcccaaa gtcgttatag
    11161 agcaaatgat tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg
    11221 aactaatgga tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag
    11281 aggaaccttt tgcagcatta gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa
    11341 tttatcaacc attgaagatt ccaagaacta tctatttggc aacaatgaat gcttttacta
    11401 gatttattca tgaacatttc caagtaacag gaagctatcg ctttgaccgt ggtttttgga
    11461 tttggcatta cactagtggc ttgatctttc ggattggtga attggagtac gaaatgacat
    11521 attttccgaa agggtacacg gatctaagat tagaaggaaa gcagtgcctt tctcttcata
    11581 ttccttccga tgcagattta tctcagaata agataagaga tagttacgaa agtgccattc
    11641 gtttttttga actttatttt cctgattacc attaccaagt gatgtacaca gatacttggt
    11701 tactatcacc caatcttact aaatggttga aaaaagagtc aaaaatctgt ctttttgcag
    11761 ccgattatcg attattatca gttgatgaac aggacgatag tggagtccct tggatctttg
    11821 gacgagtaga tgcccaaata catgattatc cagaaagtac gagtttacag cgacaagcaa
    11881 aagagcaatt gcttgcaggc gagcacattg gctctggctt aggtattctt acttcataag
    11941 tttagtactt cgaaatagtt taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc
    12001 acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa
    12061 ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc
    12121 ttctgtccca gcctcttttt tagtgttcta tcgaacacac agtacgttca ataggtaaac
    12181 tagctataac aatagatatt aaagttaacg gacaagaggg ccttttttag gtagctaaaa
    12241 cgtaataatg aaaatttatt tccagagata ggagtcaatc agctagtctg atcgactgtt
    12301 ttaacaagtt gactttcatt ttaacttttg ataccattta aaacacaaat cgtaattgtt
    12361 tcgatttgtg ttttttagca tgactacaat caaagaagaa agtgggtcgt ccatatggaa
    12421 aaaacagata tctcaagtgc ataccgccgc ttgaaaagcc caaatatcaa aacacgaaaa
    12481 cgtgcgctga agatcatcaa agatgtgaaa cggaatagtg ggaaaaggta agaagaagga
    12541 aacttaatta aaaaaatagc cgaaaaacgt ttttcggcta ttttcaataa tcggtttttg
    12601 tgtaaggttt ccgaaagaac tgctgttgat tttccatttt taacatatga taaattggta
    12661 gaatctttct tttcaaattg ataccattcc ctaattccag ttatatagtt aactacagaa
    12721 ccacaaagca cgtacgttta taaaagtatt atctgtagtt atttttcaat tccctttcaa
    12781 cctcttattt tgaccagtag tattgatcac tttacctttt aacacctttg ttcgtttttg
    12841 aaacattttt agtcaagaat ggaaacgtaa ctacataatc aaaggttttg cgaaatattt
    12901 catgaaatat ttcatgtaga agcgagcaat acggttgcaa ataaccatta tcgtttaata
    12961 agcagttacg aaagctccca atatctctaa cgttcagctc actccatttc ttttcttcaa
    13021 aaaaatcagt gattcgtatc tctttctatt tgtcgaaaaa gttaacttta aaaagtaacg
    13081 ccgaaaaaat atgcaaaaaa ataaaaaagc agttactatt gctagaataa catcaaggaa
    13141 taataagttt tggttttcag ataatagtcg ataagttgtg tattggaatt ttagatacag
    13201 taataaagta tttttcatga aattttgtga gaatcactat tacaagtatc tttaataaaa
    13261 ttaatggtta aggaggaact taaagtagaa tgtaaatgaa tagttgtagt cagtcgattg
    13321 tgatactaga ctcaaagttg caggaataag tttttcaatt tgtgattgga agataaaaaa
    13381 tgaaaaagaa gggctttttt ttatactttg ttgattgtag tgtttatttt ccctctgttt
    13441 agccaagtaa cattttctat gactcaacca gtttcaagtg aggaacatga taaccttttt
    13501 cgtgctacag aagagtttaa gaaatatcag gaaaatattg ttccggagac tccattagct
    13561 agtgagccaa ttactgatat tgatggaaac caaataggat ggtatataca gtacgaagtt
    13621 atggtggtaa ataatgactc aatagtaaaa aatacagtag gcttagagct aacaccgatt
    13681 gaaaaggtga gttcattgtt aacgtatatt tatactgatg aaagtgacga gactgagatc
    13741 attttaacgg attactcaaa aattgctact gataaaaagt atatattgtg gatgtagaca
    13801 ctagtgaaat actttatgat atagaaattg atgaaaattt actagatgta gcacaagaaa
    13861 tcgatcatca aaagcaggaa gctattcgat atgctggtag aaaagggaaa agaaaatatt
    13921 tattacgtga atgtagcaaa agttagagaa gatgaaaatg agtggaaaga attcaagagt
    13981 cgctactcaa ttaactcgac tccgacattt acagtttaca gagaaggatc gatagaaaaa
    14041 actgtttttt ggactaaaga aagtggaatg tctttagctg aagtagaaga gtttttggat
    14101 tatgttagta tgcaacagta agggagttct tattatgaaa aattttgaca catattatgt
    14161 tattgttttt tcgatatctt tgctgttaat attactaact ttaatgcagt ataaagagta
    14221 tccagagtat tttattttaa cttttgtata tttagtaagt agctacacac ttctatataa
    14281 attcagaagt tcttctagag tttctaaagt ttgtttctta attagcttat tgattctata
    14341 atatttttga tttatatagg aaattaaaaa tattatagaa tcaacactca gagaacgggt
    14401 tggtttttaa tagatttaat aagtactgat aatgaaattt ctcctggttg ggaaaacgaa
    14461 gttggttacc ctgtagaaga attttttcaa gattactttg cggagtatgc cgataggata
    14521 atgagagatt acgtaacaga taaaactcaa gaagtcatca atcaatccca gtcattaaat
    14581 gaattatctg ataaggcgaa agaagaagca gttgccatta tttcttcagg gattagtgac
    14641 tatattgata tgagtggtga aatcaattat ggcgataaag acaaaattgt acgcatgata
    14701 aaagctttta tcaaaacttt tgatgtgaag gcagatctta agtttaatga tacttaccta
    14761 gtaggaaaat taattaatat ccttgggaca gagaaagata cggaaacgtt tatttcctct
    14821 ttgtctatgt tgtttacaga taagcatttt tcaactcagc ttggtggaga ggaactagtg
    14881 gaaatctttt aaccattacg cccagcatta ataaaagtat atcaaaaaag tactcctgat
    14941 gatttttaag attggtttga gaaataatgt gaaaacacta tttttaatgt taattataga
    15001 tagagatgaa cgcgtcatat ggttaaaaga atgaaaaagt caaagataca ctgctatatt
    15061 tcactaagga tatagcagtg tatctttcat gtcgtttcac tggcagtttt ttataaaagg
    15121 aggtgtgcat ttcagttgct tttctttggt agttagttga atcgtttaag aattaacgga
    15181 tgtggcagac aggtgctctg tccaaaaacc agtaaaatct ttgatggttc catctttatt
    15241 tagctgtgcc tgttcaaata ctgttttgtt tttaccgaat ggatcaattg caataattgt
    15301 tccgatcccc gcacgtttgg cagcaagtaa tcctgagtaa gcatcttcga taacaagaca
    15361 atcagccgga gctaagctca gtttgtctgc cgctttaaga aaaatatccg gttgcggttt
    15421 cccagggaaa gagccgtcgt catagacaat cttgtcccag tcaaaccagc gggctaaatc
    15481 gaaaatatca aaataaaaag caacattctc tttgacagta gccgtggcaa tcgttaatgg
    15541 aatctgttgg tcagtcagct ggtcaagagt cgcttcaagc ccttttgtca actgaagctc
    15601 gttttcattt tgcaagcaaa gcatacgata atagctttct ttttcttctg acaatacggc
    15661 gatctcagag gcggtcaaat gctctgaaat gaaatgtttc aaaatctccg tattcgtccg
    15721 accatgaata ttttttaaaa tatcctcgtc tgataaacca tcttttgtat gatcatgtat
    15781 catatgcagc caagcagctt catgcagatg agagtccaaa aacattgttc cgttgaaatc
    15841 aaatataatc cctttcatag ttacgctcct atagtggtat tttcggttga atcagcaatt
    15901 tgcagatcga catattgtgc atagtgctct tgaacctccc gcgagatctg gcgatccgta
    15961 atgagcagat cgaaatcatg caaattataa aacacataaa aatcatctcg attgaatttt
    16021 ttattatcaa cgagtagata tttcttacgg gaattattta gagcgatctg ctgtgcttct
    16081 ccttcttcgg tactgtaagt agagatcgct tcgttgtgaa cgccatttga actgataaat
    16141 gcttttgtaa actttagtct tcttaaattg tcgttggtga tcggtccgac aaatgcgccg
    16201 gtatttttcc ggaaatcacc gccaatcagt agaatttctg gtccatctgc ttgagcaaga
    16261 atatcaaaaa ctggataatt atttgtaatg atccgaattt tcttgttcag taaatgcgtc
    16321 gccaatagtt cgattgttgt acctggtcct aagaaaatcg tttctccctc gtgaatcaag
    16381 gaggctgctg tggcagcaat ttcttttttt tcttgaatct gcagagtggc tttttcagtg
    16441 tgagaaagtt cttgatctaa agtgtaggaa agactttgag cccctccatg gacacgcagc
    16501 agcttgcccg atttttctaa ttcatctaaa tctctgcgaa cggtcatatc agaaacatct
    16561 aattgattga tgatttcatt aacggtaagg attccttttt tattgaccat ttctacgata
    16621 gtaagcaatc tttctttttt caacattgtc ttcacctcta atttaaggat aatctatcat
    16681 ataaacggac aacagtcaat ctaattcgaa caaaagtaaa catttagggt ggtttttatg
    16741 tttgttacaa tagattttat tgctgttttc tggtttttat gattatttgt gtttgttttt
    16801 gttgacaaat gttttggatg cgtttacaat gcaatcagtt agaaagagcg aaaactgatt
    16861 atagatttga ggagagatgt acatgcttaa attaccagaa gattttattt ttggcggagc
    16921 cacagcagcc tatcaggttg aaggagccac taaggaaggt ggcaaaggcg ctgttgcttg
    16981 ggatgacttt ttagaggaac aagggcgatt cagtcccgat ccggcaagtg atttttatca
    17041 tcaatatgca aaggacattg aattgtgtga acgttttggt gtaaatggtc taagattatc
    17101 cattgcttgg agccgcattt ttcctgatgg agctgggaaa ccaaatccag aaggaatcgc
    17161 tttttatcat cgtgtgttcg aagaatgtaa gaaaagaaac gtaacgccgt ttgtgacgtt
    17221 gcatcatttt gataccccta aacgtttgtt tgaccatgga gattttttga atcgagaaac
    17281 aatagaagca tttgtttcct atgccatttt ttgttttcat gaattcaaag aagtcaaggt
    17341 gtggagtacc tttaatgaaa tctatccagt tgcaaccaat caatatttgt taggtgtttt
    17401 cccaccagga atcaaatatg attttacaaa gatcgtcgcc tgtcttcata atatgatggt
    17461 tgctcatgcc cgtgtagtga actatttcaa agaaaacgaa cttccgggtg aaattggtgt
    17521 agttcactca ttagagacga aatatgcggc aacagacgcg ccggaagata agcatgctgc
    17581 ctttttagac gatgcattgt cgatccgttt cttattagat gctacctatt taggctatta
    17641 ttcaactgaa accttgactg ctcttgatga gatatgtgaa gcaaatcagg ccagctatca
    17701 ttttcctgaa gaagatttcg ttgaattgaa aaaagcatcc acgcgtaatg actacttagg
    17761 gatcaatcac taccaatgcc attttgtcaa agcttacgat ggagaaaatg caatccatca
    17821 taacggtacg ggtgaaaaag ggacctctgt ttataaagtg aagggaatcg gagaacgtat
    17881 ttataaagaa ggaattcctc gaactgattg ggactggctg atttatccag aaggtctata
    17941 tgacttgttg ctgcgaatta aaagcgatta cccacattac aacaaaatct atatcactga
    18001 gaacgggatg gggtataaag atcagtttga agatggcatt attatggatc agccgagaat
    18061 cgactatttg agagtctatt tggaatcctt aagcaaggcg ataacagccg gggtaaatgt
    18121 gaaggggtat tttttgtggt cgcttatgga tcttttctca tggacaaatg ggtataacaa
    18181 aaggtatggc ttgttttatg ttgattttga aacccagaag cgttacccaa aagaatcagc
    18241 ttactggtac aagttggtca gtgaaaccaa aacgattatt taataagtga taatggagga
    18301 agaaaagatg aataaagagg aattacaaat gcttggtttt gagatcgttg catattcagg
    18361 cgacgctcgc agtacattat tgaaattatt gaaagaagta agacaaggaa actttgaaca
    18421 tgttgattcg gcgttgaaag aagccgacga aaatttaacc cttgcacata attcgcaaac
    18481 aaaaatttta gcggaagaag catctgggaa agagatggag atgggattca tttttatcca
    18541 tgggcaggat catttaatga ccactttatt gctgagagat ctgattcaag actttattgt
    18601 actgtacaga cagaatcaag gataggagaa gaacgctatg gatggaatca ttaaacaaat
    18661 cgaaaaaggg aaaccatttt ttgaaaaaat ctcacgtaat agttatttag gagccgttcg
    18721 tgacggattt ttaacagcga tgccagctat tttattttca agcattttca ttttgattgc
    18781 tgcggttcct gagatttttg gttaccaatg gcctgaatct gtctccacat ggctatggaa
    18841 agtctataat tattcgatgg gtgttgtagg gttactcgtt tcagcaacca ctgctcgctg
    18901 cttggctgat tcgatgaatc gtcgcttacc gaaaaacaaa gtaatcaaca ccacctctgt
    18961 gatgctggcc tctgtggtaa gtttcctgtt gttaagcgtc tctccgattg aaggtggatt
    19021 ttcaacggaa tatatgggga caaagggact actggcttcc tttgtcgctg catttttaac
    19081 agttaattta tataagtttt gtgtgctgaa agatattacc attaaaatgc ctaaagaagt
    19141 tcctggaacg atttcgcaaa ccttccgaga tatttttcct ttctcttttt cagtatttgc
    19201 ggccgtttta attgatttag gcattcgaca aatcttccaa atctcatttg cagaagcaat
    19261 cattacggtg ctgcagccgc tatttacagc agctgacggg tacttaggaa tcgctattat
    19321 ctggggagcg atggcgctgt tttggtttgt cggtgttcat ggaccgtcga tcgtggagcc
    19381 ggcaattgcg gcaattatct atgccaatgt ggaaaccaat ctgcaattgt tcaattccgg
    19441 tgaacaagct tctaaagtac tgacggtggg tttagggaat tttgtaggaa ctatgggagg
    19501 aactggggca acattggttg ttccttttat cttcctactc tttgctaagt ctaagcagtt
    19561 gaaggcagtt gggaaagctt cctttattcc agtaagtttc gctgtcaacg aaccattgct
    19621 atttgcggca cccatgattt tgaaccctta tttttttatt ccatttttgt taacacctgt
    19681 tgtaaacgtt tgcctgttca agttttttgt cgatgtattg ggcatgaaca gctttatgta
    19741 cgttttgcct tgggcaactc cagcaccgat cggtttggtt ctaggaacgg gcatgggtat
    19801 cttagctttt gttctagcag cggttctaat cgtcgttgac ttcctgatct atctaccatt
    19861 ttgcaaagca tacgatgctg tcttagtaga gaaagaagca caaaaagcag aggtgatcgc
    19921 aaccgaggaa ggtgcttctg ccgatgtacg tgaggaagta cctgtggcag cgagtcaagt
    19981 tatcagcgat gcaaataaag aaatcaatgt gttggtactt tgtgctggcg cagggacaag
    20041 tgcgatgtta gctaatgctt taaaaaaagg tgcatctgaa tcgaaagcac cgatcagtgc
    20101 aatggcgggt gcttatggtt ctcattacga aatgctgcag gattttgata tggtggtctt
    20161 agcaccgcaa gtcaattctt actatgatga tatcaaagaa gacaccgatc gcttaggaat
    20221 caagctggtt gcgacaaaag gagcagaata tatcggatta acgagagatc ctgaaaaagc
    20281 tgttcagttt attctgaacc aatttcaata aaggaggagc cataatgaaa atgggaattt
    20341 ggttgcgtaa agctgtttta tttgcagctg tgttaggggc tttgggggct gtttcaaaaa
    20401 ctgttgaagc atctgccagc gaatttattc gcggagcaga tatttcgatc ctgtcagata
    20461 tggaaaaatc tggtgcaaaa tattatgaag acgatatttc aaaagatgcc ttagagattt
    20521 taaaaaataa tggtgtcaac tatgttcgac tgcgtttgtg gcaggatcct tataatagta
    20581 atggagaaag ttatggagca gggacaaatg atctggaaac gacgattgac cttgcaaaac
    20641 gagcaaaaaa tctgggactt aaggtgctgc tggattttca ctacagtgat ttttgggttg
    20701 atcctgggaa acagaatctt cctaaagcat ggcaaggtct gacctttgaa gaaatgaata
    20761 cggcattgta cgactataca aagaacgttc tgtcggaaat gaagcagctg gacgtttatc
    20821 ctgatatggt tcagatcggc aatgaactga atagcggaat gctctggcct tatggcaagt
    20881 cttggggaga aggcggggga gaatttgatc gattggctgc ttttttgaag tctggtatcc
    20941 aagctgttcg agacactcaa cctaaaacaa ctccagtaat gctccactta gctgatggtg
    21001 gcgatacagg agcatttact tggtggtttg atgaaatcac gagtcgaggt gtgtcatttg
    21061 atttaattgg agtgtcgtat tatccgtatt ggcacggttc aatgaatgaa cttcaaacca
    21121 atatgaatgc gatcagccaa cggtatggta aagaagtgat cgtagttgaa acagcttatg
    21181 ggcatacaac agcgaatgcc gatactatgc caaatgcttt tggtgaagcc gaagcagctg
    21241 cggggggata tgaaccaaca ccgacaggac aagcagagta tttgctggat cttgccgatc
    21301 ggattcaggc agtaccgaat aatcgcgggg cgggcttctt ctattgggaa cctctttggt
    21361 acaacggaaa tgtgagttgg gcaacacaag caggtatgaa ttatctcagt gttcaatcca
    21421 ctatgggaaa cgaatgggac aaccaagcga tgtttgattt tgctggaaac gccttgccaa
    21481 gcttgcgtgc gtttaaacaa gcaggggcgc aaataaatct agttaaaaat gcaagtttcg
    21541 aaagcgatgg accaacgacc agtccttcca attggcaaac ctggtttcaa caaggaaccg
    21601 ctaagggtac gatcaaaacg gaagcgggca ctttttttgg ggactacaaa ctaacttttt
    21661 ggcaggatca agcatacgag gcatcagctt atcaaaatgt tagtggcata agcaaaggaa
    21721 cttacacatt gagtgcctat gtaatgtctg ggggagagtt tgatactaat cagttatata
    21781 tcaaaaatta tggtggagcg gaactaaata ctgcggttgt taaaacaagt gaatggacaa
    21841 aaattacaat caaagacatt ccaattacaa caggtgtttg tgaaattggg gtttatagca
    21901 aggcttcagg aaatgcttgg acgaacgttg attttgtttc attggtgaaa caatagactg
    21961 cggatcgttt cgattcaaga tcggaggttc ccttcagcca catcgttagc gtagaaggca
    22021 ggaatcgaac ctgcacctcg cctttgccaa aaataaccag aaacgatttc cagtcatttt
    22081 taccaaaacg agtaaagcac cagacttcat ctacactttg tattatgcta aagagagttc
    22141 aacgatttcg taagaagcaa tgaaaaaatt gcaaaaagtt gtgcagataa cataaagttt
    22201 gtttaagaaa gggtaacctt agaattccgt cgtgaatatg atttgagcac ttagatggat
    22261 agaaaaaagt tctcggaaaa cagttcgttt ttcgagaact tctttttatg ataagaatga
    22321 aacaaattaa cttatattat agtctctttt ggtagggaac catcttattt ttaatgaaaa
    22381 tcaagcattt tagctattga agaatgttga tttataggga ttccccctcc atattatgtt
    22441 ggctctttat tcccactctg ttttcaacgt aaagtgtata aaacagaatg cgatactatc
    22501 aagggagtta aaacaatata cagaacagta aaacactata aataaaaata aaagtgcaat
    22561 aaaagtgcaa tttttgcacc taatcatact ctgtttatat atgaaaacta gattctgaat
    22621 ccaatatttg gttaacgaga ggcgttcgaa tttgaacgtc ttttatatat tactttcaaa
    22681 taaatatgag gtagaatact atctattttc tttttaaccc aattgttata atggagagtg
    22741 taaaatattt tgtgtaaata gaaaaaagga agtcccttct gtagaataga gttaccacaa
    22801 cacattcaca gaagagagga cttccctatg aacgatttta ctacagaaat tctaaagact
    22861 ctagcgaaca aaggcgattt gaatgaatta ttccgtgtcc atttggagaa agcagtcaat
    22921 acgcttctca aaacggagtt aacggctttc ctagattacg aaaagtatga tcgcattggt
    22981 tttaacacgg gtaattctcg taacggctcc tatgaccgta cggtcaagac cgagtatggg
    23041 gaacttcatc tccagattcc gcgcgaccgc aatggtgagt tcaagcaaca gactgttcct
    23101 gcttatagac ggacgaatga cacgttagag gagaccgtca ttcacctctt ccgaaaaggt
    23161 attaccatgt cggaaatcgc agacttgatt gagaaaatgt atgggcatta ctacacgccc
    23221 caaaccatgt ccaatataac aaaatcattt acagaagagg taacggcgtt taatgggcgg
    23281 gagcttcatg accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg
    23341 aaaaccgtcg ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag
    23401 gaagtattga gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta
    23461 ttggaccttc aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag
    23521 gggatggtag gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta
    23581 cacgtttccc gtaatatcag tcataaagtg cgtgtcgatg atcgtaagga agtctgtgat
    23641 gattttaaaa tggtgtatca agcctcatct aaagaggtgg cgttggaagc acgtggtact
    23701 tttgcggaaa aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat
    23761 cacttgctca ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac
    23821 ttgattgaat cctttaataa acaaatcaag aaatacagcc accgcaagga acagttccaa
    23881 aatgaagagt cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc
    23941 ctaggtcgca gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctaagc
    24001 caactgattg agaattagaa acaatctgca gtagggaaga atcatttaca caaaattatt
    24061 gacgctccct tataatgaga acttttacta acataattta tgataaactt actatataat
    24121 catattaatt aaatagttag gagatctgct tatgtttata tctactcttc aaattcgaaa
    24181 ttttagaaat tatgaatccg aaatctttcg attcgataat gagacaaata ctattatagg
    24241 tgagaatgat tcagggaaaa ccaatgctct tactgcattg agaatcttat tagatgatag
    24301 ttattactat agtagtaaaa cactaaaaga atcagatttt tttcatggaa ttcaaaatgg
    24361 gtggcaaggg cattggatta ttatctctgc gacatttgaa ggaatcagcg aagaagaatt
    24421 tgacaatgag atttgtgctt ccatttcttt aaattctgaa agtcaaacaa tcttagaaga
    24481 attgatttca aatgcagata aaggtgtagg tagtatttct ctatttatta gacctaacaa
    24541 agctattaga aagcaattat ttgatatttc cactgaatta gactctcatc aatttaacga
    24601 atttagaaat tctattcgtc tatccgacta tgaattttac tacacatcta aatcaaatct
    24661 aaatttctgt atcaatgaaa attatgataa attggtagga agactcaatg acaatatagc
    24721 tagtaatcct gaagaagacg atgaagctct gttgggctca agaattgaca tgtcagatat
    24781 tttcaagcat atttcggttg tttatgttga cgctcttcga gatgttctta gagaaatgaa
    24841 aaacaatcga aacccagtca aaagaatcat ggaaacaatt gaatcaaaaa tatcatcaga
    24901 taatgttgag tcattaaaaa caattataca gcaattaaat gagactatta ccagtgttcc
    24961 tgaaattagg aaaattggcg aaaatattaa tcggcaacta aatagcataa ttggttcagt
    25021 gtattctcct aatcttcttc tttcctcaac tatgtcagat gatatgggtt ctttagcaaa
    25081 atttctttca atgaagccag aacaaaatat cgacttagac ttactagggt taggacatct
    25141 aaatatgatt tatttagctt tgaaaatagt cgaatttgaa gcgtgtcgtt caagagaact
    25201 acttaatatt atgttgattg aagaaccaga agctcatatt catcatcata ttcaaaaaac
    25261 tctgttcgaa ggattaaatc ttcaaaaaaa ctatactcag attctcatga ccacacattc
    25321 tgtacattta gctgaagtgt ctgaaatatc tcggatgaat gtattaaaat cgtttaatgg
    25381 caaatctatc gttatgaatc cagcaaataa tctgaactca tttgctaaag aaaagttaaa
    25441 aaagaacaat ctaaatctaa taaacgcagt agagagatat ttagatgtta aaagaaatgg
    25501 attgcttttt tctaaaggtg ttatcttagt tgaaggagat gctgaagaaa tacttattcc
    25561 tcaaatagta aaaaaattat ttggtataaa cttagacgaa cttggaatag gtttgattaa
    25621 tgttggtagt acttcgtttg aatatatcgc tagcttgttt gacgatgata gaatacagag
    25681 aaagtgtgct attgtaactg atttagatga acaggcaatc ccatcagaac atcgattgta
    25741 taaaagcact gcacaagaga aaggtcaagc aagaaaagaa aaattatcct ccctttttaa
    25801 tgaaaacaat tgggttaatt gtttttatgc taatacaaca tttgaaattg agtttttaga
    25861 tgtgaataaa gaaaatattc taacttatgt cagtccaatt atagatgacg cattcaaaga
    25921 taaaaaaaca ataactgctt ataaagaaga aatacaaagt gaacaatatt tagaaagaaa
    25981 tttagcgatg ttaaaactag ccaatcatgt tggtaagggg tggtttgcaa ttgagttagg
    26041 tgaaaaaatt gactttttaa tcaaaattcc taagtatatt cttaatgcta ttgcttttgc
    26101 atgtcaggaa atagtagatc tctcaattta taaaaaaatt ctactccata caatcgactt
    26161 gtacgaggaa actacagaat tgtcagcttt atcagctagt ttaaaaacaa gtgatacagc
    26221 tgatttgaat atagcaataa tttctttatt agagtacttt ggtaaagatg aggatgttgt
    26281 gtatctaatc aatcagatag aaaattacac aaaaatactt tatgctgagg tttcaaaatg
    26341 acaaatatct tcgaaaaaat cgtaaatgat ttaaatttag aacaaaaaga tgttgtggaa
    26401 agtgaagata acctttatgt aaatgcatgt cctgggagtg ggaaaacaag ggtacttaca
    26461 aggaaaattg cttaccaagc aattaaacac gatggatcac taaaaagaat aattgctatc
    26521 acatacacta atagagctgc tgaagaaatt aaggaacgcc taagtttact tagtatagat
    26581 gatgatatta atatttgggt ggggacaata catcagtttt gtcttgaatt tattatttat
    26641 ccttttaaaa tgaatctctc aagagtatca aaaggattta caattattga tgattatact
    26701 caaagagctt acgtcagcca aattaataat atgctgaatt taaatctcaa aacgtatgaa
    26761 ctgaacaaaa ttgatttgtc actttctcat gattttaaaa ttaatgaagt tcagtatact
    26821 caagtttctg aaatgtatca cactatgttg gaaaaaaata aagaaattga ttttgatatg
    26881 attttactag tagcgtataa aattttaact gtaaacagta acgttgcacg taatattgct
    26941 agtaatataa gggcgattta tgtggatgaa tttcaagata ctagagaatt acaatacaat
    27001 gtaatagcta aattacttca aaacaaccct caaattcagt caatgtttgt tggagatatt
    27061 gaccaagcaa tttatggatc attagatggt atagctaaat cagttgaaga actagaaatg
    27121 ctaactagtc acacttttca atcaaagacg ctacatggct gttacaggtc taatcagaga
    27181 ttagtagact tctactctaa ttttcagagt tgtccttacg agattgaaag cagagggaat
    27241 aataaaaacg accgtggttt tataagttat gattgcaaag ttactaagga ggatttaccc
    27301 aacataataa aaaatataat aaaggaaaag attgaaagcg gcattcatga aaaagatatt
    27361 tgtgtacttg ctcctcaaca ctatcctctg ttttcactgg gtgagtattt aaagaaagaa
    27421 ttaccgtatt gcaattttga tgctataaat atatcaccaa ttaaggtaaa taatcatagt
    27481 ttatttttca agttagcaat tttgtacttt actgaatcag gtgaaaaggt tcgaagaaga
    27541 aagctaattg caaacgatat tttaattatc ttaattgtca aattaagtta gacagatcat
    27601 caatacttac gtaactcaaa aatacttcca aagg
//
LOCUS       DAYYFU010000031        26318 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000031 DAYYFU010000000
VERSION     DAYYFU010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.031"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..308
                     /locus_tag="ACHA34_001602"
     CDS             <1..308
                     /locus_tag="ACHA34_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8792228.1"
                     /translation="IRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLI
                     EGFNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            781..1335
                     /locus_tag="ACHA34_001603"
     CDS             781..1335
                     /locus_tag="ACHA34_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8792229.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(1508..1981)
                     /locus_tag="ACHA34_001604"
     CDS             complement(1508..1981)
                     /locus_tag="ACHA34_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8792230.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1993..2940
                     /locus_tag="ACHA34_001605"
                     /pseudo
     CDS             1993..2940
                     /locus_tag="ACHA34_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            3211..3646
                     /locus_tag="ACHA34_001606"
                     /pseudo
     CDS             3211..3646
                     /locus_tag="ACHA34_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            3662..4495
                     /locus_tag="ACHA34_001607"
     CDS             3662..4495
                     /locus_tag="ACHA34_001607"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8792231.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            4740..5765
                     /locus_tag="ACHA34_001608"
     CDS             4740..5765
                     /locus_tag="ACHA34_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8792232.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5903..6028
                     /locus_tag="ACHA34_001609"
     CDS             5903..6028
                     /locus_tag="ACHA34_001609"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792233.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            6053..7168
                     /locus_tag="ACHA34_001610"
                     /pseudo
     CDS             6053..7168
                     /locus_tag="ACHA34_001610"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            7251..8147
                     /locus_tag="ACHA34_001611"
                     /pseudo
     CDS             7251..8147
                     /locus_tag="ACHA34_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            8163..8987
                     /locus_tag="ACHA34_001612"
     CDS             8163..8987
                     /locus_tag="ACHA34_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8792234.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            9019..10329
                     /locus_tag="ACHA34_001613"
     CDS             9019..10329
                     /locus_tag="ACHA34_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFM8792235.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            10477..11736
                     /locus_tag="ACHA34_001614"
     CDS             10477..11736
                     /locus_tag="ACHA34_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8792236.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11746..12618
                     /locus_tag="ACHA34_001615"
     CDS             11746..12618
                     /locus_tag="ACHA34_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8792237.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12630..13460
                     /locus_tag="ACHA34_001616"
     CDS             12630..13460
                     /locus_tag="ACHA34_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8792238.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            13500..15683
                     /locus_tag="ACHA34_001617"
     CDS             13500..15683
                     /locus_tag="ACHA34_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8792239.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15744..16964
                     /locus_tag="ACHA34_001618"
     CDS             15744..16964
                     /locus_tag="ACHA34_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8792240.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16961..18421
                     /gene="gtfA"
                     /locus_tag="ACHA34_001619"
     CDS             16961..18421
                     /gene="gtfA"
                     /locus_tag="ACHA34_001619"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFM8792241.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            18498..19604
                     /locus_tag="ACHA34_001620"
                     /pseudo
     CDS             18498..19604
                     /locus_tag="ACHA34_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19926..20135
                     /locus_tag="ACHA34_001621"
     CDS             19926..20135
                     /locus_tag="ACHA34_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8792242.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            20128..20277
                     /locus_tag="ACHA34_001622"
     CDS             20128..20277
                     /locus_tag="ACHA34_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFM8792243.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            20431..21096
                     /locus_tag="ACHA34_001623"
     CDS             20431..21096
                     /locus_tag="ACHA34_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8792244.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(21380..22465)
                     /gene="xerS"
                     /locus_tag="ACHA34_001624"
     CDS             complement(21380..22465)
                     /gene="xerS"
                     /locus_tag="ACHA34_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFM8792245.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22799..23032
                     /locus_tag="ACHA34_001625"
     CDS             22799..23032
                     /locus_tag="ACHA34_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFM8792246.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            23029..23436
                     /locus_tag="ACHA34_001626"
     CDS             23029..23436
                     /locus_tag="ACHA34_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8792247.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(24573..25091)
                     /locus_tag="ACHA34_001627"
     CDS             complement(24573..25091)
                     /locus_tag="ACHA34_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFM8792248.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            25458..25706
                     /locus_tag="ACHA34_001628"
     CDS             25458..25706
                     /locus_tag="ACHA34_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792249.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
     gene            26209..>26318
                     /locus_tag="ACHA34_001629"
     CDS             26209..>26318
                     /locus_tag="ACHA34_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8792250.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT         8997 a         4012 c         4879 g         8430 t
ORIGIN      
        1 aaataaggtt tatgatagat aaatggaaaa agcagtatcc acgagtggtt aaattgctca
       61 tgaatcctgc tatattaacc ttttataatt tccctccgtc aatcagaaga actatttatt
      121 caactaattt gattgaaggt tttaacaaac aattaaagaa atataccaaa agaaaagagc
      181 aatttcctaa tgaagaatct ctggagagat ttctagtatc tcaattcaac aactataatc
      241 aaaagtttct atgccgagtt cataaaggtt ttaaagaaat acatgataca ttagaatcaa
      301 tgttttaact tgatcaacag aatgggcttt tccatttaca caaaattatt gacgctcccg
      361 acaaaattat gatcttgcgt aaagttataa aatattaata aaaatatttt attaatgaaa
      421 agcttggtag cacggttatt catatgctaa tttatttcac tttatagtaa ggggagttct
      481 tagtatattg ttcaaactta aatttcatat aatttaatta tgtaaattag ctattctaag
      541 aacttctacg gataactgcc cgtctaaaat ttaaataaga gcttagaatc ttcatataag
      601 taaggttaat ttttttgtac aaaaaagtcg gaatattctg agttactgaa atgatataaa
      661 atcataaact aaatcccttg attttattga ctttcaaaag tttatatata taagttaatg
      721 attatggtaa aatatataca taatcattaa ctcagaatat ttttaagaag ggaaacaata
      781 gtgagctata atgtacaacc gttaaggaca aatgaagaga tacaagattt tcagttttgg
      841 ctaagaagaa caagtaatcc ggaaagagat agttttttat ttttgtttgg aatcaataat
      901 gggttacgta tgtcagatat tataggatta aaagttggag atatacgagg caaaagtaag
      961 cctattattg ttgaacataa aacaggtaag cgaaaaccga tttttataga taatttgaga
     1021 gaagaaattt tattatatac tgaaggtaaa gaagaaaatg attggttatt tccgagtcgg
     1081 caacaaggtc gacatataac aagggatcgt gtctatcaga tatatgcaga tatagcagaa
     1141 aaattaggac gagatgatat aggaacacat acattaagaa aaacttttgg gtatcactac
     1201 tacaagaaaa ctagagacat tgctacctta atgtttattt ttaatcatag ctcgcaagca
     1261 attacaaaaa ggtacattgg tataactgaa gatgaaatag gagcatcact tcgtggattt
     1321 aaactaggtg tataatttaa aaatttttat aaacagtaaa gacaaagatt aaaagctatt
     1381 tttatctaga acgattttat ttcataaagt tcgcggaata aaagctgggg ttactggtac
     1441 ttggtatagg ttcgtgttat ataaactaag taattaaata acatttttag gttggaaaag
     1501 gatgaaatta aaaaaagaga atcaatacgg tctcaaaaac gcaaacgctg cggaataaga
     1561 aaaatactga taactcaacg tttagcagac tatctaatag ggatagtctg cttttttcgt
     1621 ttttgaccat acttttgacc ttctgcaaac ttccttgctt ttcctctgta gaactcatga
     1681 aatctgcaaa acgaattgcc gtttcctctg tctgcttttt cgcgatatgg gtgtatatat
     1741 catccatagt ggttttaata tctgtatgtc caagtcgaac atacacttct ttgacagacg
     1801 caccagattc aaagagcaga ctacaatggg tatgacgaaa accatgaagt gtgatatgtt
     1861 ttaagttatt ctctttaatg atgattttca aattgtgatt tacatagtct aaataaaggg
     1921 gcttattttc ttctgatgaa aacaaaagtt gttcaccatc ttttataggt ttgattctca
     1981 tttttagata ttatgcccgc acgtattcat cacatttcta ttgtgaatcg ctttattcga
     2041 ccgacatttg acttttatca taatatactc ggtttaaaat tactaatgaa aacaattaat
     2101 caagacgatc ataccatgta tcacctgttt ttttcagata atcaccaccg tgtgggaact
     2161 gaactgactt tttttgaagt acaagaaggg aacaaccagt tttttggaac caatacgatt
     2221 gaacgaacga tactaaaagt tccttccgaa gcatcattgc atttttggga aatatatttt
     2281 gaaacacaag gcgtttgcca ttatggaatc gaatcatttt ctggacgacc tatcttacgt
     2341 tttgaagggc cagatgcaac acgattagct ttagttccct tacgcgaatt tgaagatatt
     2401 gatgattatt tcccgtatgt cacagaccag atccctacag agcatgccat tttaggaatt
     2461 gatgccattc aactacgcgt acaatattcc gatgcgactg aacgagagtt attgtcggta
     2521 ggctggtagc acaaagaata cgtcagtttc ttttcgggtc atcaacgagt gacaatctta
     2581 gaaaatcaag acactcagtt ctatcaagaa gttcagatta ttcaagatcg tgagaatcca
     2641 gttgccattg aagggattgg tagcgtccat catgttgcgt ttggcgtaga aaataaatct
     2701 gatttacaac gcatcgacaa acaattacaa gagcgcaatt ttataaattc aggaataaaa
     2761 gatcgtgaat tttttgtatc actttattat cgtgacccaa atcaattatt gattgaaatt
     2821 gctacagggg aaggaaacct agatgcaaaa gcgtatgaaa atcaaagtcc acgctttgaa
     2881 gaaattcctt tatttttacc gcagtactta aatttcattc gtgaacaagt ggagcaataa
     2941 ttagctaatc aaatccatga cgaagagtaa gaggttgact cataagtcaa aaaataaaga
     3001 aaaacgcatc aaatccattc taaagaatgt tgatttgatg cggttcctat ttttataaac
     3061 tatcattttc gactaataaa actacttatg agtcagcctc tctttttttc ttgattttta
     3121 tacctctttc taggtaaact aaacatctgc caaaaatacc gtgtctcatt gaaacattct
     3181 caactatttc aaaataaagg ggagtaccaa atgaacgagt atattgaaat tatccacgca
     3241 agagaaacaa cttaaaaatg tcagtctcaa aattcccaaa aataaaatta gtatttttac
     3301 cagcgtatct ggttcaggaa aatcatcgat tgtttttgat actattaccc aagaagctgg
     3361 tcgtcaactg aatgacacct atgatagttt tacccgttta tttttaccta aatacagtcg
     3421 tccagaggtt gatgagattg ataatctttc tacggcgatt gtggtcaatc aaaagaagat
     3481 tggtggcagt gcgcgctcaa cgttagggac gattactaac atcaatgcgt tatttcgtgt
     3541 gttgttttca tgttttgcaa cacctagttt aggatatgca aatgcttatt catttaatga
     3601 tcctagtgga atgtgttctg tctgtcaggg aatcggtaag acgattgcat ttttaatagg
     3661 attgctttat ttttcggcta ttgataatgc tgattttgta ttttctgatc catttaccgc
     3721 agaagttttg tctggaattg aggaacaggc aaagaatcat ggttattttg tattggtgca
     3781 taatgttaat tcagcagaag atgttaaaac tattcagaga aattggcgtt ttgaggggtt
     3841 cattgttgtt ggtgtacgtg taaaagaatt tttggaatta aataacgttt ttgatggtcc
     3901 tgttgtttat attgatacgc atctttcaaa agagacatta agagaacttg aacaacaaat
     3961 gaatcgttta tttgtaaata caaatgatta ccaagctagt acattggcag tggaacattt
     4021 gattcattat ggtcatgaga aaatagcgtt tctttctttt gactacattg taggtgaacc
     4081 aagtgttatt caagaacgac atcaagctta tatcgataca ttaaagaaat atcatttaac
     4141 ctttaaatcg cattggatat ataacaactc tgaatttgag cgaatttatc aagaattagc
     4201 tgaatttact gcaattgttg ttactggaga ttatttagcg gctaaattag taaaattttt
     4261 aaaaaataag aataaacgag tgatggaaca attatcgatt attggatttg atgatattac
     4321 ttttgcagaa ttaatggatc ctgcgttgac aacaatacga ctaaatccag gtaacaaggg
     4381 ggcgattgct tttcaccaac tgattggact ggccaaacaa gaagaaataa aaaatcaagt
     4441 actactatta gagggagaat tgatttctcg agactctgtt cacaaaatca aataaaaaag
     4501 ctctgtgaaa taagtatagt taacaataaa atatccgaac gatagagaga atgaactgtg
     4561 atctagcagt tcctcgtcgc aaattcacta gaattagcat tatttactct aaattgttcg
     4621 gatatttttt cttgttttta attatgatta cagtgcctat ttttaacgaa atttgcttat
     4681 tttcaaaatg tatgattata tgttagtaaa cactttcaaa tatgaaaaga ggagctatta
     4741 tggtaggaat tagggatgtt gcaaaacggg cgggtgtgtc cattgcaaca gtatcgcgcg
     4801 tgctaaatga ggatccgaca ctttcagtaa ccgatgaaac caaacgaaag attgcagaat
     4861 cggttaattt ttatcattat gtcaaaaaaa gtcccgttgc aaaaaattta acaaatattg
     4921 cattaataac gacagtttct gaagtagatg agttggaaga tccctatttt cgaacgattc
     4981 gcagagggat tcaagtcgaa gcagaacaaa gcaaagtcaa tttgaagaaa attattcgtt
     5041 tgtcagaaac accgttggag caagacgatt tgctaaaatg tcaaggtgtg ttgattattg
     5101 ggcaggtgct atcttctgtg attgaagaag tatcagcaat taattcgaat gtcgtagtga
     5161 ttgatgaccc tagtattgaa gctgaagtag atgcagttta tactgattta acaaaagcaa
     5221 cgaatcagca tttagatcga ttgtataaaa aaggacaccg aaacattgcg tttatcggag
     5281 gaggacgtgt tcgtttagat agccaaggaa ataaacacgt gtcagatgat gattatcgaa
     5341 aaatagccta tgagacgtgg atgaaacaaa aaggattaga aaaatatagt caaatttatt
     5401 tagaaggctg gaccacactt gacggaatgc aggcagcaga tttacttttg aaaaaacatg
     5461 ctaaagataa gttgccgact gctatcatgg taggaaatga tcctattgct gtgggggttt
     5521 atcgttcact tcaaaaaaat ggtctacata ttccagaaga tatttcgatt gtgagttttg
     5581 ataatattga agttgctgaa tttttaacac ctgcattaaa tacggtgaat gtgaatacag
     5641 aagaaattgg aaaactagca gttcgcatgg ttaatgaacg aataaaagaa gttagaaatg
     5701 tttcgatcaa agtgattgtt tctaataata ttattgttag agaaagtgaa aaaaatatcg
     5761 attaaaaaag taaacatcca aaaaagggtg tttacttttt tgtttactaa tatttttagt
     5821 aaaaaatctc gacaaaacat taagtaaaca gttatggtgt tgttaacaaa aagaaaacgc
     5881 attcaaaaca ggaggaatta agatgaaaca tttcaaaaaa atagctttaa cagggatagc
     5941 tgttttaact gggacattac tcgcagcatg tggtggggga gatagtaatt ctaaagacgg
     6001 gcaagtaact ttaagctatg cgagttgaga tactgaccaa gctccaggac taagaaaaat
     6061 gttagatgaa tttgaaaaac aaaatcctga tatcaaagta gaaatggaga caacgccttg
     6121 ggatcaatat tgggttaaat tagaagctgc aacaacggga ggtaatatgc cagatgtagt
     6181 aaccatgcac tctagcgaat cctataaata tatgtcacag aatgctttga tgggacttga
     6241 tgatgttatc aaagaaaatg atattgacat gggaaacttt actgaaggaa ttgctgattt
     6301 ctatacgctt gaagacgaac tatacgcaat ccctaaagat gcttctgtag tggggctatg
     6361 gtacaacaaa gaattatttg ataatgcagg tgtcgattat ccagatgaaa catggacatg
     6421 ggaaacactc agacaagctg caattgattt aacagatgaa tcaaaaggca tctatggttt
     6481 tgcatcacat attggaacag agactggcta ctggccattt atttatcaaa atgggggaga
     6541 agtatttgcc gatgacaata caaaatcagg tttgaatacc ccagaagcac aagaagcttt
     6601 agctttttat cgagatattg cacttgttga tcatgcttca ccaacaatta cagaattaca
     6661 agatcctgat aagttaagtc gttttcaatc aggtagatta gcaatgatga ttgaaggaaa
     6721 ctggtttgca ccgggatttt tgaaaaatga ctatctagtt gaaaatgttg cttctgccgt
     6781 gttacctaag ggaaaagtta atgcaacaat tacaaatgga cttggttggg ctgccagtgc
     6841 gagtacaaaa tacccagaag aagtgaaaaa actaatggca tttttagcta gtgaggacgc
     6901 caataaaatc caagcggaaa cgggggcatc aatcccagca ctaaaaggct ttgaagacga
     6961 gtgggctgct tcttcagata caattgattt gagtgctttt ggaaaaatgt tggagtatgg
     7021 agtccctcgt ccatacaata aagatggatt acgagctgaa caagcagaag ttcaaattat
     7081 taaccgtatt ctgtctggtg aagtaactgt tgaagatgga accgctcaaa ttgaaaaaga
     7141 agtcaatgcg atattgagcg gaaattaaat tagttaatta tacaatatac aggcgaaagg
     7201 attctatttc gtctgtatat taatggatga ataccaaaaa gggggatatt atgaaaaatc
     7261 gaatttttgg aaaatatcgt aaagatcaag tgatatgggg ctatattttt atttttccaa
     7321 cggcattagg attaatttta ttaaatatat ggccagcaat tcaaacagca attttaagtt
     7381 ttcaaaaaac ggtagggttc ggtgatactg aatgggtcgg attaagaaat taccaaaaaa
     7441 tgtttgcaga cggtgaagtt tttcagtctt tattaaacac attaatttat gcagtggttt
     7501 cgatacctag tattgttgta ttatccttgt tggtggcagt tttaatgaat agaaaagtta
     7561 aaggattgtc gatttatcgg acaatttatt ttctaccagt cgttgctgca ccatccgcag
     7621 tcgcaatgat ttggcgctgg atgttcaata atgattatgg actgattaat aacatgttgg
     7681 ctaaaatagg tctcgatgga ccagcatggt taacggatcc atcgattgcg atttattcta
     7741 ttatcattgt aggagtttgg agtgctattg gttataacat ggtgttatta ctagccggct
     7801 tataagaaat tcctaaagat tattatgaag cagcggaaat tgatggtgct agtccaatcc
     7861 agcagttttt caacattacg ttaccattag ttacaccaac attatttttt gtagttgtaa
     7921 caacagttat caatgctttc caagtatttg atgttatttt tatgatgatt actcctaata
     7981 gtaccgccat gtttaaatca caatcattag cctatttatt ctataaacat tcatttattt
     8041 taaatgacaa gggatatggt tcggcaatcg ttatgttcct tttagtgatt atattaatta
     8101 ttactgcaat tcaaatgaag ttgcagaaaa agtgggtaaa ctattaagga ggctatcaaa
     8161 ttatgaaaaa taaatttttt tcaaatgcgc caatccatat catgcttatt ttaggtggta
     8221 ttgcaatggc attacctttt atttggatga ttttaacttc agggaaaacg ttggttgaat
     8281 ctacccaaat tccacctaca attttccctg agaaatttca gctaagtaac tatcaacaag
     8341 tacttgattt attaccattc atggcttttt atatcaatac atttttaatg gttgttggac
     8401 gagtgattgg ctcggtttta ttttcagcaa tggctgcata tgcttgtgcg cgtctaagtt
     8461 ttccagggaa aaatatcctt ttcagcttag tacttattca aatgatgatt cccggttcca
     8521 tttttattac tccacagtat ttattagcac aaaaattggg tattttaaat acaattccag
     8581 cattaatatt accaggggta gttagtgcgt ttggtacgtt tttattaaga caatttttct
     8641 taggtttacc tgatgaattg gaggaagcgg cctatttaga aggtgccaat atttggacga
     8701 tctttttcaa aattatgttg ccgcttgcta aatcaggatt ggttgcactt ggaatattca
     8761 cagcgttgtt tgcatttaaa gatttaatgt ggccattgat tgtcaatact tcaattgata
     8821 agatgccttt agctccggga ttagcaaact tacaaggaca atatgccacg aacttcccac
     8881 aattaatggc aggttcgtta ttagcaattt ggccaatagt tgtgatattc atcgttttcc
     8941 aaagaaaatt cattgaagga attgctacaa caggtagtaa attataaaac tattaattag
     9001 tgattggaga gtaagtacat gacaaaaatt acatttattg gtgcaggaag tactattttt
     9061 gcaaaaaacg tattaggcga tgcgatgtta actccaagtc tacaagatgc acatattgca
     9121 ttgtacgata ttgatgaaaa acgactaaaa gaatcaaatt taatgcttaa aaatattaat
     9181 aaaaattcga atgaaaatcg tgctgatatt gtttcttatt atgatcgcaa agaagcgtta
     9241 agagaggctg attttgttat caatgctatt caagtgggtg gttataaacc ttcaacagtg
     9301 attgattttg atattcctaa aaaatatggg ttgcgacaaa ctattggtga caccatgggg
     9361 attggtggga tttttagaac cctacggaca ctaccagtaa tgttagactt tgctaaagaa
     9421 atggaagaag tttgtccaaa tgcttggtta ttgaactata caaatccgat ggcgatgtta
     9481 accatgggtg ttttaaaagc gacgagtatt aagactgttg gtttatgtca tagcgttcaa
     9541 gtatgcgtac cagaattgtt tgagcattta ggaatagccg atcaatatga cttaaatgac
     9601 tttcaatgga aaattgcagg cattaatcat atggcattct tgttggaaat tacaaaaaat
     9661 ggggaagatt tctatccaac aattcgtcag ttagctgcag aaaaaccaaa tccacatcca
     9721 gattctgtac gttttgaatt gttgaaacgt tttggttact atattacaga atcaagtgaa
     9781 cacaatgcag aataccatcc atactttatt aaatcaaaat acccagaatt gattgacgaa
     9841 ttagggattc cgttggatga atacttacgt cgttgtgaac aacaaattaa tgattgggaa
     9901 agcatgcgag aggacattgt aaataatggg gagttaacac atacgagaag ccgtgaatat
     9961 gcttcatata tcatggattc tattacgaca ggaactccgt cagtgattgc aggaaatgtc
    10021 ttaaataaag gcttaatcac aaacttaccg acagatgttt gtgtggaagt accttgttta
    10081 gtcgataaaa atggcgtgca accaacctat gtcggtgaat tgccaacaca attagctgct
    10141 ttaaatcgga cgaatatcaa tgttcaagaa ctgacagtag aagccgcttt aacactagat
    10201 aaatcaaaaa tttatcaagc agcgtattta gatccacatc tatcatctga attgtcttta
    10261 acagatatca aaaatttagt ggatgattta atcgaagcac atggggacta tttacctgaa
    10321 tataattaaa aagaagctga aagagtaagg aattttcaat tccttactct tttcttagtt
    10381 aaatttagta aaaatattga caaaacactt agtaaacagt tacattagtg ttaacaaaaa
    10441 gaaaacgcat tcaaattgta taaggaggag cataaaatga agtggtctaa aaaaattatt
    10501 ttagcttgta tagcactagg agcaacgatc tctttgactg cttgtggaaa aaatcaggaa
    10561 gatgcaagcg gtaaggtaga aatagagttt tttaatcaaa aaaaagaaat gactcaaacg
    10621 attcaggaga ttgctaaaga ttttgaagca aaaaaccctg atattcatgt aaaagttgtg
    10681 gatgtaccga atgctggtga agtaattaaa acacgtatgc ttgcaggcga tgtaccagat
    10741 gtcattaatc tttatcctca aagtatagaa ttgaaagaat gggcgaaagc tggttattta
    10801 gaggacttaa ccgaagaacc gtatttagag aatatcaaaa atggatatgc acaacgtttt
    10861 gctattgagg atcgtgtata tagcatacca ttaaccgcta atgtctatgg tttttactat
    10921 aacaaaactg cttttgagga aatggggatt caagcaccag aaacttgggc agaatttgaa
    10981 aaaatagtag ctgagattaa agatcaaaac aaagtaccgt ttgcgattgc aggagcagaa
    11041 ggctggactt taaatggtta tcaccaacta gctatagcga ctgctgctgg tggtgaagag
    11101 gaagcgaaca atgtttggcg tttctcagaa gttaatggaa ttaatgctga aagcaaagaa
    11161 atgcaaaaag attttaatcg attagatttg ttaagagaac caaaagcttt gcaaaacaac
    11221 tggcaaggtg ccggttataa cgatacagta gttactttta caaaaggtga tgcgttgatt
    11281 atgccgaatg gttcatgggc aatgccgatg attcattcac aaaatccaga ttttgaagta
    11341 ggaacttttc cttttccagc agatgaagca ggtcaaagtc taacgattgg cgcaggagac
    11401 ctagccttat cagtttctgc aacttctcaa cataaagaag aagccaaacg ttttgtagaa
    11461 tatatgacaa cacctgaagc gatgcagaaa tattatgatg tagatggtgc accgtgtgct
    11521 gttgagggcg ttatcgagaa tactcaggat tcaccattaa gtggtttaac tgaacttgct
    11581 tttactgatc gtcatttagt ttggttagcc aaagattgga atagtgaaaa tgatttttat
    11641 accttaacaa caaattattt acacaatggc aatcaaaaag ccatgattaa cgcattaaat
    11701 gctttcttta atccaatgaa agctgacgga aggtaggaaa ataaaatgaa aataaaagaa
    11761 attttaaatc gttactgggc aatcattttt gtagcaattc caattatttt gcagatgatt
    11821 tttttctact ttccaatggt tcaaggtgct ttttacagtt taactaattg gactggtctg
    11881 acttataatt ttgattttgt gggtttgaac aattataaga ttcttttttc tgatccgaag
    11941 tttatcaaat cgattggctt cactttaatt gtgacgcttt gcatgattgc tggacaaatt
    12001 ggttttggta ttttgattgc gcgggcgttg aacgcaaata ttaaagggaa aacattcttt
    12061 agagcatggt tcttttttcc agcggtctta tcgggcgtgg ttgtttcttt agtatttaaa
    12121 caaattttta actatggttt accaaaaatt ggtagtatgt taggcattga atttttacaa
    12181 accagtcttt taggaacaac atggggagcg atttttgcag taatttttgt tttactttgg
    12241 caaggaattg ccatgccagt catcattttc ttagcgggtt ttcaaagtat tccacaagaa
    12301 attattgaag ctgctagaat ggatggggcg aatgataagc aaattttttg gaatattgaa
    12361 atcacctact tattaccaag tatttcaatg gtatttatta tggcattaaa aggtggctta
    12421 acagcatttg atcaaatttt tgctttaact ggtggtggac caagcgatgc cacaacatct
    12481 ttggggttac ttgtttataa ttatgctttt aaaaacaatc agtttggtta tgcgaatgct
    12541 attgctttag tcttatttat cttaattggg attgtttcac tggcacaatt aaaattatcg
    12601 aaaaaatttg aaatctagga ggtttcccaa tgaaaacatc aaaagaaaaa aatatttcta
    12661 aatacttgct tttattatta gggggcttca tgattttagt tcccttagtt tcaacgctat
    12721 tcggttcatt taaaacaaca cccgatatta tgaataattt ctttcgtttt cctaatccag
    12781 cgactttgga aaatttccga agattgttag cggatggaat tggtcattac ttttggaatt
    12841 cggcaatcat cacaatcatt gcagtagttg ctgtgacagc aattattcct atggctgctt
    12901 attcactagc aagaaaaatg actcgtggga aagcatacgg attgatgtat ttatttttaa
    12961 ttttaggaat ttttgttcct ttccaagtaa ttatgattcc gattaccgtt atgatgagta
    13021 aaattggttt agcaaatatg tggggattaa tcttactcta tctcacttat gccgtaccac
    13081 agacactatt tttatatgtt ggttttatta aactaaatgt gcctatcagt ctggatgagg
    13141 ctgctgaaat tgatggggct agtcacttta caacttattt caaaattgtt ttcccaatgt
    13201 taaaaccaat gcatgcgacg actattattc ttaatgctct atggttttgg aatgatttca
    13261 tgctaccatt attaatttta aatcgtgatt ctaatatgtg gacactacca ttgtttcaat
    13321 ataattatac gggacaatat tttaacgact atggtccaag ttttgcttcc tatacagtag
    13381 gaattgtcac gattacacta gtttatcttg ctttccaaag aagtattata gctggaatga
    13441 gcaatggagc agtgaaataa ccaataaatt attctcatta taaaggagtt cataaactta
    13501 tgattatatt tgacaatcac acacaaacat ttcatttaca aaataaaaaa attagctatc
    13561 tattaggaat cgaagaaaat aattatttaa cgcatcttta ttttggaaaa aagattacgc
    13621 actattctgg tgggtatcgc tacccccgaa cagatcgttc cttctcaccc aacaatgcaa
    13681 atgcgcaaga tcgtttatta tctttagaca cattaccatt agaatttcca ggatatggac
    13741 atggtgattt tagagaaccg gcgcataata ttaaattagg aaacggtagt cgtgtaaatg
    13801 attttcgtta tgatagctac gaaattcaaa aaggaaagcc cagcttagcg ggattgccag
    13861 ccttatatac ggaagatgat gatgaagctg aaacgctgat tattcaattg aaagacgcga
    13921 ttagtggatt gactttacag ttgttctaca caatttatca aaatcacagt gtaattattc
    13981 gttctacgca attagtaaat aatagtgatg aaacagtgga gattaaccgc ttagctagtc
    14041 aatcaattga ttttccaaat cgtccactcg agttaattca tttaaatggt acctgggcaa
    14101 gagaacgtca aatgactaga gaaaaaattc atacagggat aaaagtatta gatagtaaac
    14161 gcggatcaag cagccatcaa caaaatccgt ttgttgcttt agtagatgcc aaaacagatg
    14221 aatttcaagg agaggcatat ggatttaatt tagtttatag cggtaatcac gaaacggtga
    14281 ttcaaaaaga tccatttgac caaacacggg tcattatggg aattaattca tttaattttg
    14341 gttggcaact aaaaactggc gactcatttc aaacgccaga agtcgtgatg gtttatagta
    14401 atgaaggtct gaatacgtta tcacagacgt atcatgaatt gtataatcac cgtttaattc
    14461 gtggaaacta tcgtttaaaa gaacgtccca cgttaatcaa caactgggaa gcaacgtatt
    14521 ttgattttaa cgaagaaaaa atcatgaata ttgtcgatga tgcgaagaac ttgggcattg
    14581 aaatgtttgt tttagatgat ggttggtttg gcggtcgtga aaatgacttc acttcattag
    14641 gtgattgggt tgaaacagaa ggcaaacttg cttgtggttt ggaaaattta gccaagcaag
    14701 tgcatgataa cgggatgaaa tttgggattt ggtttgaacc ggaaatgatt tctgaagata
    14761 gtgaactatt tcgagcacat ccagattggg cattgggtat tccaggtcgt gaccgctcaa
    14821 ttagtcgaaa tcaatatgtt cttgattttt caagacaaga tgttcgtgat aatatctatc
    14881 aacaaatgac ggcgattttt gatcgtgtac cgattgatta tgtgaagtgg gatatgaatc
    14941 gaaatatgac agaagtctat tctgtcttgt tggactcaac gatgcaaggt gaagtggctc
    15001 atcgttatat gttagggtta tatgaatttt tagaaaagtt aacaacgaat tatccaacta
    15061 ttttatttga aagttgttcg ggtggaggcg gtcgttttga tgcaggtttc ttgtattaca
    15121 tgccacaaac ttggacgagc gacaataccg atgccgtggc tcgtctgaaa attcaatatg
    15181 gaaccagctt agtgtatcca atttcaagta tgggtgcgca tgtttcagca gtcccaaatc
    15241 atcaaacagg aagagaaacg agtttaacta ttcgtggcaa tgctgcgatg gctggtgttt
    15301 ttggttatga gctagatcta acgcaattaa cttctgaaga aaaagcagaa attaaagaac
    15361 aagttgcctt ttataaaaag catcgccaac ttttgcaata tggaactttt tatcgcttgg
    15421 aaagtccgtt tgaaggcaat gatactgcat ggatgtttgt ctcaaaagac caaacagaag
    15481 ccatggtctt ttatttccga gtattagcag aagctgcgca tccattagtc acattgaaat
    15541 tagcaggatt agcagaagaa gcaacttatc aaatggaaga tgtaacattt gctggcgatg
    15601 aattgatgaa tctcggtttt tatattgact cagaactaca tggtgattat gcgacacaac
    15661 gcttttattt gaaacagata tagtagcttg aatttacaat ataagtgcaa caccaaaaga
    15721 gactcattcc aaacgaagaa aatatgtttt atttacacgc atttactaaa aagcagcctg
    15781 atttgaattg ggaaaatgaa gaagtacgtg aagaaatcta tgcaatgatt aactattggt
    15841 tagataaagg actaggcgga tttagaatcg atgcgatatt aaacatcaaa aaaaaactag
    15901 agtatggcat ctttgaatct gatggagaag atgggttagc cttcattggg ccctggattc
    15961 ttaatcagtc gggcattgaa gtgtggttaa aagaaatgag agagcggaca tttaaaccac
    16021 ataatagtat gacggtagca gaagccgatg ttcctaatga acgtttagat gaatatattg
    16081 gtgaagatgg ctattattcg atggtttttg attttagtta tacggatatt gatgtacctg
    16141 aaactggtga atggtttaaa gatagccaat ggacatggac ggatatgcgt accaatattt
    16201 ttacgaatca attagttacg caagataaag gttggggcgc cttatattta gaaaaccacg
    16261 atcaaccacg ttcaattaat aaatatattc cagaagaatg gatcaatgat tatagtaaaa
    16321 aaatgttggc gacgcttttt atgttactaa gaggtacccc ttttatttat caaggacaag
    16381 aactaggaat gaccaacata gaaatggcat cattagaaga ttttgatgat gtagctacgc
    16441 attctcagta taaacgggca ttagaatatg gattggcacc agagcaagca ttgaaagctg
    16501 tcaataaacg aagtcgggat aattctcgca cacctatgca atggaatacg caaaaaaatg
    16561 ccagattctc aacttctgat aatgtatggt taaaagtaaa tcctaattat ccagaactca
    16621 atgcagaagc tcagttaacc aatcaacagt cggtattcaa ttattatcat caattaatta
    16681 aattaagaaa aaatagtgta tacaaagacg tgctaatcta cggacaattt ctaccagtga
    16741 aagataaaaa tgaacatatt ttgctttatg aacgccaatt agaagataaa atcgtattag
    16801 ttatcttaaa cgttacagca acagaacaag aatattatgt agatgaacag taccaacaag
    16861 tattaatcaa taattatgaa gatgttttat tagatcaaag tcgaaaattg cgtttacgac
    16921 cgtttgaaag tattgtatta gctaattatg gaggacaatt atgaaaatta aaaacgaagc
    16981 aatgctcatc acctatgcag atagtttagg aaaaaacatg ggcgaattaa aacaagtctt
    17041 agacaaacat ttacaaggtg ttattggtgg tgtacatctg ttgccttttt ttccatcgac
    17101 aggtgatcga ggctttgcgc cgagtgatta tacgacagtt gatccagctc ttggaacatg
    17161 ggaagatgtg gaagcattag gtgaagatta ttatttgatg tttgatttta tgattaatca
    17221 catttctcgt gaatcaaaat ttttccaaga tttcaaaaaa aaccatgagc agtcaccata
    17281 caaagaaatg tttatccgta ttcatgaatt tttcccagaa aatcgaccaa cacaagaaga
    17341 tattgattta atctacaagc gtaaagacaa agcgccgttt caaacggtag aatttgcgga
    17401 tggtacgaca gaagaggttt ggaatacctt tggggaagaa caaattgatt tagatgtgac
    17461 caaagaagtt gtgaaagaat ttattcgaga aaccatcaaa gatatggcga accatggctg
    17521 ttctttaatt cgattagatg cgtttgctta tgcgattaaa aaattagata ccaatgattt
    17581 cttcgtggaa ccagatattt gggaactttt agacgaagtg cgtcaagaag cagcgaaata
    17641 tgacgtcgaa ttattaccag aaatccatga gcattattcc attcaaatga aaattgccaa
    17701 tcatgattat tatatctatg acttcgcctt gccaatggtc actctgtatt cactttatag
    17761 tggaaaatca gaacgtttag ccaattggtt gaaaatgagt ccgatgaagc agtttacaac
    17821 attggatacg catgatggaa ttggtgttgt ggacgctcgt gatttattaa cagatgaaga
    17881 attggattac acctctgagg aattgtataa agtcggggcg aatgtgaaga aggtctattc
    17941 aagtgccaat tacaataatt tagatattta tcaaattaac agcacctatt atagtgcatt
    18001 aggcgataat gaccgtagct atctgttagc acgtgcgatt caagtatttg cgccagggat
    18061 tccacaaatt tattacgttg ggttattagc aggaaaaaat gatattgaac tgttggaagc
    18121 aacgaaagaa gggcgtaata ttaatcgtca ttactatgat ttagccgaaa ttgaagaaga
    18181 agtgcaacgg ccagtgattc aacaattatt tgcactttta aaatttagaa atgaatctgc
    18241 ggcgtttgat ttggatggaa cgattgaagt agcaacgcca tcagaatcag aaatagtgat
    18301 tacacgtaaa aataaaaacg gtagtgatag tgcaacatta acggctaact tggatacgaa
    18361 agaatttatc attgagtcta aagatataaa aattttcact agtgttgcat taaaatttta
    18421 acctattcca tagaacaaaa aatcctttat actagcactt ggatgactta tccaagacca
    18481 ataaaaagga ctcatacatg gataagtata aaacaaatac agcatttaat aaatggtttt
    18541 ctgccataaa attaggaaaa ctttctgtca actcccaaaa atgtattttt gattacaatt
    18601 cgtatcgtaa aaaactgtct tttgaagctg tgctcaagct ctttctctac gcagttaatg
    18661 aagaaaaaga aagtttgcgt gatttgagta cagctcttat cgaccgatcg ctccaactag
    18721 aaaccgatgt gtcatcgatt agtcataccc aattgtctcg agcatttaat gcacttgaac
    18781 ctaaagtgtt agaagaaatt tttcaacaac tgttagaaaa agttcaacat caaatcaaac
    18841 caaccaagcg aaatcatctg tatctgattg attccagtac gttttcgctt agtttaaaac
    18901 gtcataagtg ggcgactttc cgtcaaacaa aatcaggtgt caagttacac ttaaactatt
    18961 gttatatgga cgatactacg atgtatccaa ctgattttac cttaacaaat gcccatgaac
    19021 atgatctcaa tcaactacca gtattggtga atcaaccaga ggcaacttac gtgtttgatc
    19081 gtgggtatta ggactttgaa aagatggatc agatgcattg ggatggctat ttcttcgtca
    19141 ctcggattaa aaaaaacacc aaagttcacg tgatagatac tctggaaaca tcgccagaaa
    19201 cagaaattct acgagacgag ctggttcgtt taggatctaa aacctatcta accgccaatt
    19261 tccgactagt cactgttcaa gataagaacg gacgtgtgtt tcaatttatc accaatcgaa
    19321 tggacgtctc ttcaaaagaa atctctgata tgtatcacgc tcgttggcaa atcgaattgt
    19381 ttttcaagca catcaaacaa cacatgacca ttaaaacctt cttttctcaa agtgaaaaag
    19441 gcgtgcagaa tcaattgatt ttaacgatga tttccgctct cctcacgttt ctcatcaaat
    19501 tggagacgaa aacggaaaaa tcagtgttcc aaatcaaacg attctttcgt tatttactgt
    19561 ttcagccctt tgaatgttgt ctagaaaaat taattcccac atgagaaaaa agaaatttgg
    19621 ctcgttactt ttggttttca gttgaataac agataaaaaa ttttctggaa aaagtcatcc
    19681 tctgcgtcag cacagttttt tttgacttat tttcagagga aatagttttc agaatattct
    19741 aaaatgtttt atgcaacact agtgatattt tttagaatta gatttctaat gtgcattttt
    19801 tgagtattaa ggagcaagta tacaaagatt tataaagctg attaaatttg aataaaaata
    19861 tgcaggaacg ctactatttt tagtagtgtt cttttttata tttgaataaa aaatgtggtg
    19921 gttaaatggt ggttataaca aaaaatgaat taatgaaatt aggttatggg aagtatcagt
    19981 ctgagagaat catacgacag gcaaaagcaa ttatggtttc aaaaagctat acctattacg
    20041 aaaatagaag attaggacta gtacctgttg aatctgtaga agaagtttta ggtatgaaga
    20101 tagaggtaga ggaggatttt cagaatcatg gttaatttta aaaaagataa aaatgggaaa
    20161 tatcatttta ctgctagttt gggattcgac aaacgaacag ggaaaagagt acaaaaaaac
    20221 gtagaggggt taaaacagtt aaagaagaaa aaaagcgtat accgaacttg tttctaatta
    20281 tggaaaagaa gcagaaactt ctcaaagtac tatgttgttt gaggagtttt tttataaatt
    20341 attcctacct tattatcaag ggaaagtaga agaaagaaca tataaaaatc ggttatctac
    20401 aatcaaaaaa acttttctta tttctttaaa atggagttaa gagaaatacg tccaattgac
    20461 attcaaaagt ggcaaaataa tttattgagc aaatataaaa attcttatgt aagaaacatg
    20521 tatgggattt ttcagatgtc attagatcga gcagttgttt tgggactact tgaaaaaaat
    20581 cctgcaaaag ttattggaaa tgtgaagaaa aggaaaacta cagttgattt ttggacaaaa
    20641 gaagaattta caaaagttat ttcaactttt tatattgatg atttgtacca acattttggt
    20701 tttatttgta tgtggttact ttttatgacc gttatgcgtt tgggagaagc cactgcatta
    20761 acttggaaag atattgattt tgaaaataaa aacttgcgtg tgaataaaac actctattat
    20821 aagagagcca aaacctatga gtttgtagaa cccgaaacgg cttctagtat tcgtacaatc
    20881 gaactagacg acaatacaat taccttttta aaagcttgga aggagcgtca agtttcaata
    20941 ggaggcatgt cgtttgtact ttcttataat ggaacaccta ctcagaaata tacaatccgt
    21001 catattatga gaagacatgc aacgatcgtt ggtgttcata taatccgcat acacggatta
    21061 aggcatagtc atgcttcttt gcttatatca caataaaaag atgcacttcg aattaaagaa
    21121 aggttaaaac atgctgatgt acaaacgact ttaggaactt atggtcattt gtatctaaat
    21181 tcattgagag agataacaaa tgatcccaca gattatatcc aaactaattt ttctaaaatc
    21241 aaaccgacac ctatttacga taataaaaaa taaatgataa acaaaagtag gatcacacca
    21301 aaagaaacac actagaaaaa ctataataca ggcttttcta gcgtgttgtt tcttatttcc
    21361 gcctatttat acattatact cataaagcat ttaatgcatc gatcgtagcc gcattgtcta
    21421 tatgggtata taaagttgtt gtattggtac ttgaatgtcc caattgttgg gcagttaata
    21481 ccaatgaatt tgtctgttgg tagagtctag tagctaatgt atgacgcagt ttgtgagggc
    21541 tgacttgaat tctaaagcct tttgaatatt ttgctaccat ccgttcgatt gcaataccgg
    21601 atatacgctt ggcttctcct tggtaaattg ctagaaataa agctttttcg ttttctgtta
    21661 ctttatatcg ttctttccga atagctagat aattagctaa atattccatt gcgaatgctg
    21721 ctatattgac agcatctctt ttatttcctt ttctagttac taccacatta ttcttatcta
    21781 aagataggtc atctaaatta atatttataa cctcggagag tctcaatcca gatcctaaaa
    21841 tcagtgcatt gatcgccagg tctctttcta catctcgttg ataatagatc aaagctcgtt
    21901 tactcaaagt atttttatat tcattggcaa tataatctag ataattttgt gtatccttat
    21961 ctagaaataa ttttttctca atggcattgg cacgtgcagc taacgtagtt ttatctgtga
    22021 tggttgctac ttgatccata ggattattgt ctaagtatgg ttttcctaga ggattattgg
    22081 aacttttcga aagatattta aatagaacct ttaatgccgt gatatttcta ttgatagtag
    22141 aagtagatag acctaagtta ttattatctt tgagcttagt ttttgttgtc tcttttttta
    22201 cattttcttt tgtgcgacgt ttcaaatgag atttgtacaa gtttaggtca taggcagtta
    22261 actgtcttat cgaggtgata gaagtatcag gaacgataga ctcactcaga agccattcat
    22321 aaaagagctt ataatttttt agatattcta aagtggttcg aggtgattga tctttatcct
    22381 cacgatcttg aacaaaatct tttatataat cgggcatatg aagtatgatt gtatctattt
    22441 tttgatatat ttctgtattt ttcatgtcat tcatcctttc aataagtagg ggagaagagt
    22501 atctctataa ttggactata ccaattatag aatgttttgt caattggtat gctctaaaat
    22561 ttattaattt gtttcaaaaa aagcatataa acaggtatac gtttaataat ttaaaataaa
    22621 tggtatacat aaatagggaa ggaaatagaa gggagcaatc taaatttttt gaattttctt
    22681 aattaattat atagaaagaa caatctttct atagagttag acatgagagg gactatgctt
    22741 gatttaaaat caacaagcgt atatactgta tatacaaaga aaaaggaggg agaagactat
    22801 ggcaattaaa gaaagaaaat ttagggaagt ggggaactca gtagtattaa ctatttcaaa
    22861 ggagtttctt aaagaaaatg ggttaaatcc cggagatacg gtttttttag atgaagaaaa
    22921 acttcgagcg gcaattacaa aaaaacaagc cttatctgat gaaattgatt tagcaatatc
    22981 tcaagcatta actgaatatg atactacttt tagagagctt gtcgataaat gagtattcat
    23041 tacctagaag ccaaagatat tataaaaatg aatgtaatcc aaataaaaaa atactctcct
    23101 aatgaacccc ttggtgtaaa ggatgcgaat gctttagaaa tgtgtgtagg gcaacttcga
    23161 gcctcagctt ttggtgaaga ggtttatcct tcgatttatg aaaaagcagc tatcctactt
    23221 atacaattga taaagaaaca tccttttcac aatgcaaata aacgtactgc tttcttagct
    23281 acttttgtct ttttgaaaat caatggacag cttttagcta ttgatcaaaa agatgctgta
    23341 aatttagcgg tatatattgc tacgtatgat aaggatttcg atcaactaaa atatgaagtg
    23401 atcaacacta taaagacaca tacgcagccc atttaaggag ttactgtatg tgtctttata
    23461 gggttattag ctgctatagc gaaataattg aagtaaatta caaatagcta taattcaaat
    23521 ccaactatat agaaaacgta aattttctat atagttggat taagataacg actatttatt
    23581 ttatcatatt tttactatac cctatacttt tacttggtat atttatgaat aatcgcaaat
    23641 ttacccctat actactagtt tgtaagttag attaataaaa atactaaagt tatttgttct
    23701 tttattaaat acacctacaa cttataaatt gatggtattt agataacacg aaagaataaa
    23761 aataacagct gatagtaaag tttgttgacg attgctactc aatcgaaagt gagttagtgc
    23821 gtatgaactt gcttctgaaa acttatgaaa tgaaagacct tttacatctt gcagaaatcg
    23881 gtactgggat taaactaagc aataaaaact aatgttcccc tcattctttg aacttctact
    23941 atttgattta tttattctga taaatgacga tttcaccttc aggatcttca aatcccgtat
    24001 agaatttatt taagatctct tcagtatcag taataggata ggcgataggc cacataccct
    24061 ttgatggtta gaataaactg ctcaaaatct gtatcgtatg ttggctttag catattaaca
    24121 aaggctatct ccaaattata aaccataagc gttgaatcgt tcttaacaat ttcaattctt
    24181 cctaacccaa agtcagaaga tataattacc ggacttgcct tacccttcat gtgggttgtt
    24241 gagattccat atttaaaggg aattgtcggt actatcggcg gtctgaaaaa catcccaaaa
    24301 ataaaaaaat ctttcccctg ctagtctcct aagtcccttt gtagcggata tggcaccata
    24361 atctcatttt atcgaagtga gtcatggttt atctgttggc caaccaaaat tgatacaatc
    24421 taacgatgca ccctctacgg cattctaggg gagtgcactt gcattcaccc aataaaaacc
    24481 ggcagttagc aaagaactta tgtagtcgta tgttcttact gttaacatac aagaggtctc
    24541 ctttccacaa ttgcttagca gtaaacggga atttacaaag ctaatctctt ccattttctt
    24601 atctttgttc taaaagtccc aaatggagca actgtattta catgaataaa tttatatact
    24661 tcccatacag ccgttttggt tgcctcgtct gcccattttc ttttgtgtgc agtgaataga
    24721 tcttcatcac tcattgaatc aatcatcatg ttaatatcat caatattacg attgagaatt
    24781 tcctctaatt ctttcaatgg tagatgtgca taggtatttg taaaccactt atataactct
    24841 cctaactgat tccatttaaa ttgatcagaa ggcgttttaa cagataatcc atttctttca
    24901 tcattttccc attttagaat taaattcgtc caaccaactt gatatgctaa attttcagca
    24961 ggtgttctat ccacttcctc aactctttta tccttcaatt cttcaggaat agtttcaaat
    25021 tctaaaatat atttagtata agtcctattt atttcttttt ttaattcttc tttattttta
    25081 tatgttctca ttatattacc tccataaatt cctaattgtc tcctttaatt catctataat
    25141 ttcatccacg atattttaat ccgttctaca atgcatatct gatatgcatt gactgtctaa
    25201 ctcatatgtg aatgatttat aaaatatccc aacgtctcat ctaaagaaat gttgatcgtc
    25261 agcaaggagc taaaaaatac gaatatattt cttttgtagt aaaatcggac tggtttgcat
    25321 atcattttct tcgtaaatat caaaatcatg ttaactacta tcaattacta ataaaaacta
    25381 ccactttaag cgttaagaac tgttaagttt agttaagtga ggatagttca caaaatccat
    25441 tgttaaactt cacgaaagtg gtaaaactca aaatgcttta tataggatta cagcatcgct
    25501 ctttcttcga ttgcgcgttg ggtaaaaaat attatgaagt aaaaattgac gatgacacgt
    25561 tccttacggc tcatcagatg aaacagctac aaaaacgaaa tgatcaactc gaagaaaatt
    25621 taattctaaa aaagcgattg ccatattcac gcctcgctcg acgaactatt aaaggtcaag
    25681 atttacctga agatcatcct ttttaggaaa ttgaagcttt ttatgttgca caatgcattt
    25741 ataatactac aatgatgttt tcaccagata ttcttctagg gtgcggtgta atgaaacaga
    25801 tacacttgca aaagaaaatt caccgcgaat ttactaaaat tatgaataat tatgttaatc
    25861 ttccgaaatt tttttccaaa attaagggat caagctggtg ttatagattg tttaaggttg
    25921 gcaaaaaaat aacttctttt taaacaatta tagaaaaccg atactctgtt aagttggttg
    25981 acacggtgtc gatttttatg actttcttat tctgaaactt cactaacgga cagtacctaa
    26041 aaagtctata gtatggacgt ttacgagtac tattttatca aaaatgctgc tcatttcaac
    26101 ttatatgttt tagtgaacta cccccactta gcttggatag gctacaatta actagacaga
    26161 aaaattaagg tgtgtagact agaagaaaac ataccaggag gaatttttat gtctaagaga
    26221 acacgaagaa ctttttcaca agaattcaag caacaaatcg tcaatcttta cttagctgga
    26281 aagccacgtg tagaaatcat tcgagaatat gaactaac
//
LOCUS       DAYYFU010000032        26245 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000032 DAYYFU010000000
VERSION     DAYYFU010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.032"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(303..416)
                     /locus_tag="ACHA34_001630"
                     /pseudo
     CDS             complement(303..416)
                     /locus_tag="ACHA34_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(799..2031)
                     /locus_tag="ACHA34_001631"
     CDS             complement(799..2031)
                     /locus_tag="ACHA34_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFM8792251.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2285..2854
                     /locus_tag="ACHA34_001632"
     CDS             2285..2854
                     /locus_tag="ACHA34_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8792252.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3032..3472)
                     /locus_tag="ACHA34_001633"
     CDS             complement(3032..3472)
                     /locus_tag="ACHA34_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFM8792253.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3630..4394
                     /gene="map"
                     /locus_tag="ACHA34_001634"
     CDS             3630..4394
                     /gene="map"
                     /locus_tag="ACHA34_001634"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFM8792254.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4426..5349
                     /locus_tag="ACHA34_001635"
     CDS             4426..5349
                     /locus_tag="ACHA34_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM8792255.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5425..6564
                     /locus_tag="ACHA34_001636"
     CDS             5425..6564
                     /locus_tag="ACHA34_001636"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFM8792256.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6557..7357
                     /locus_tag="ACHA34_001637"
     CDS             6557..7357
                     /locus_tag="ACHA34_001637"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8792257.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7357..8184
                     /locus_tag="ACHA34_001638"
     CDS             7357..8184
                     /locus_tag="ACHA34_001638"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8792258.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8189..8896
                     /locus_tag="ACHA34_001639"
     CDS             8189..8896
                     /locus_tag="ACHA34_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8792259.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            8996..9862
                     /gene="rfbA"
                     /locus_tag="ACHA34_001640"
     CDS             8996..9862
                     /gene="rfbA"
                     /locus_tag="ACHA34_001640"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFM8792260.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9876..10448
                     /gene="rfbC"
                     /locus_tag="ACHA34_001641"
     CDS             9876..10448
                     /gene="rfbC"
                     /locus_tag="ACHA34_001641"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFM8792261.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10470..11498
                     /gene="rfbB"
                     /locus_tag="ACHA34_001642"
     CDS             10470..11498
                     /gene="rfbB"
                     /locus_tag="ACHA34_001642"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFM8792262.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11596..12447
                     /gene="rfbD"
                     /locus_tag="ACHA34_001643"
     CDS             11596..12447
                     /gene="rfbD"
                     /locus_tag="ACHA34_001643"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFM8792263.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12482..14515
                     /locus_tag="ACHA34_001644"
     CDS             12482..14515
                     /locus_tag="ACHA34_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792264.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14559..15839
                     /locus_tag="ACHA34_001645"
     CDS             14559..15839
                     /locus_tag="ACHA34_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFM8792265.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16049..16855
                     /locus_tag="ACHA34_001646"
     CDS             16049..16855
                     /locus_tag="ACHA34_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8792266.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16867..18087
                     /locus_tag="ACHA34_001647"
     CDS             16867..18087
                     /locus_tag="ACHA34_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8792267.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSNGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18077..19663
                     /locus_tag="ACHA34_001648"
     CDS             18077..19663
                     /locus_tag="ACHA34_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8792268.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19702..21840
                     /locus_tag="ACHA34_001649"
     CDS             19702..21840
                     /locus_tag="ACHA34_001649"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8792269.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(22208..23191)
                     /locus_tag="ACHA34_001650"
     CDS             complement(22208..23191)
                     /locus_tag="ACHA34_001650"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8792270.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            23569..24960
                     /locus_tag="ACHA34_001651"
     CDS             23569..24960
                     /locus_tag="ACHA34_001651"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8792271.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24976..26016
                     /locus_tag="ACHA34_001652"
     CDS             24976..26016
                     /locus_tag="ACHA34_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8792272.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            26035..>26245
                     /locus_tag="ACHA34_001653"
     CDS             26035..>26245
                     /locus_tag="ACHA34_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM8792273.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIF"
BASE COUNT         8351 a         4434 c         5333 g         8127 t
ORIGIN      
        1 agggtcaaat ttaaaccata attgtgtttt tccttccatc agcataaaag ttcacctcaa
       61 aaagagtata cgaacgaatg ttctttttgt gaaggcggaa tttaatatac tgaataatag
      121 aaaacaagta cttgtgccaa attatgtgcc aaaaaaattc gaattcaaca aaatgtaaac
      181 aaatagaaaa ccttctatat taagatttcc tgcaataaca aacacaacag aagacgtgta
      241 atagttagtc aggaacgtac aaataacccc tgtatccttt gcggtacagg gggttatttt
      301 gtataagtag acattgtagt ttctaaactg ctatgtccta aacgtttcga tacgctaagt
      361 atatttactc cttgataaag taaaatagat gcatgcgtat gtcttagtcc atcaataaac
      421 agtcgaagag aaaataggga aaaattgatt actaaagaaa aacttactag gtcgacggtg
      481 tagttgattt tcatacttca atagattcga attaaacgat ttcgattggc ttataccagt
      541 cctatccaac gaaagatata tcgttcttaa ctctagcggt ataacttgaa gctttacgta
      601 aagccaatgg gaatgaactg gagatgactt tttattattt ttagtaatag ctgatgggtt
      661 aatacttaca actttccttt tcaaagtacc atataaaaaa acggtggggt tgtttagcga
      721 aataaaggat ctaagccaat agcttagatc ctttattcta tgctttatat tattttttgc
      781 atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta ctgtagagcc
      841 atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat ctacgtgggt
      901 ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg taccgccttt
      961 cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt catcaaataa
     1021 tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta caagtgcgac
     1081 ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga catcttctcc
     1141 ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca tacccgagat
     1201 gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta ctcgatgact
     1261 gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt ctccacgagc
     1321 attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa tgatatctgt
     1381 accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt ctgctttttt
     1441 tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat agacacgagt
     1501 cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca gttctgggaa
     1561 gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga ctttatttgc
     1621 ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat aagacgctac
     1681 tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc tgatcctgct
     1741 tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta cattctcaat
     1801 acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag tccattgaac
     1861 gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag caaggggtgc
     1921 ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta cgccagtttc
     1981 tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca taataaaaat
     2041 tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta tgattcaaac
     2101 aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc atgatatatg
     2161 ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca tatcaaaaaa
     2221 acatgttaca atgaaattaa tcaatggtgg aagcgttata aaaaaattaa ggagattaaa
     2281 aaatatggca agaaaaaaga cgattactag agaacagatt ttggcagctg cttatgaagt
     2341 agctgccacc gatggattta cacgatttac tgcgagaaac atcgcgaaca aaatgaattg
     2401 ttccacgcaa ccgatttatc ttgagtttaa aaatatggat gacttgagac aagctctttt
     2461 tgagcagatc tatgatcatt tacaatatga agtgttcact gttgagcata cagggaatac
     2521 gattatcgat cttgctttta attatatcca ttttgcaaga agagagaaga agttgtatcg
     2581 ttcactgtac ttagaagaat atggtggagg aaaagagatg cagaatttct cttatactta
     2641 ctttgtgaat acagtgaaac gcgatccagc ttatgtgaat ctttcagaag agcagatcga
     2701 atctttgtat aatgggacat ggatcatagc tactgggatc gcagctttga tgtcttcaag
     2761 catcatccat ccaactgatg aacagattga aaagatgatc caagacagta tagatgctat
     2821 cttgaaattc aaagaaccaa tcgaaatcga ttgaatatac tcaaaagttc cacaaatcag
     2881 taggtaacaa gagatgtgtc aaaataagga aaagttcgtt tttcttacag cgtatgcatt
     2941 tttaactccc gaagaactct tccatgaatg caatgaataa aagagccata cagattgaat
     3001 aaatctggat ggctctttta ttgcaagtat attaaatttt tgcgactagt tctttagcga
     3061 aagcttctaa attttcgata tcttcttctt cagcattcag atccacttta acattttcgg
     3121 ctccttttga tgcgcctgtt tttgcaaaag cagcatcgaa atcatccacc gatttgcaga
     3181 attcatcgta aaaagtatca ccagatccgc agacaccgta aatttttccc aatagatcca
     3241 attcttgcaa gtcttcataa aagtctacga tctcatcagg cagatcgcca tcatcgtatg
     3301 tataagtagc tactacgcaa atatctgcct cctcgaattc atctgcatct acttgggtac
     3361 attcgttgat ttcgacttcg atatccagat tttcaaatgc ttctgcaaca atatcggcga
     3421 tttcttcagt gtttcctgtc atgctagcat atacgatttt tgctaaagcc atgatgtgcc
     3481 tcctcgttta taattattta acaattatga attataccaa aacatcattg aaaagaaaat
     3541 aaaacctaaa gacacctggg gaatgtcatt tggctaaatg tatgccgcta tgttaaaatg
     3601 agagggattt gtaaaaagga gacgaaaata tgattacttt aaaatcagca cgtgaaattg
     3661 aaatgatggc agaatctgga gctttattag cagatgtaca taaaaatctg cgtgatttta
     3721 taaaaccagg aatcacaagt tgggatattg aggtatttgt cagaaactat atcgagagtc
     3781 atggcggaat tgctgcgcaa attggctttg aagggtatga atatgcgact tgtatcagta
     3841 taaatgatga aatttgtcat ggattccctc gtaaaaagcc tttgaaaaac ggtgatttgg
     3901 taaaagtcga tatgtgtatc gatttgaaag gcggcatttc tgattcttgt tgggcctatg
     3961 tagttggaga atctacacct gagatcgacc gattgatgga agtaacgaag aaagctttgt
     4021 acatcggtat tgagcaagca caagtaggga atcggattgg tgacatcggt catgctatcc
     4081 aaacttatgt agaaggcgaa aatctagcga tcgttcgtga ttttattggt catggcgttg
     4141 gtcctacgat tcatgaagaa ccagcagttc ctcattacgg agaagctgga aaagggttgc
     4201 ggttgaaaga aggcatggtt atcacgatcg aacctatggt taataccgga acttggaaaa
     4261 tgaaaatgga tcctaacggc tggactgcgt atacaagaga cggcggactt tcttgccaat
     4321 atgaacatac attggctatt actaaagatg gtccaagaat tttgacatca caaggagaag
     4381 aaggcactta ttgatctatc cagtcaatag gtacgggaga ggtacatgaa agtttggaac
     4441 aaaataaaac aaaatgaaaa tctgatgcgc tttattgaga cgactcaaaa gcgcatcact
     4501 gattcagaaa tgagtacgac ctctgtcgtc gttgcttatt atttactctt gtctttattt
     4561 ccattgatta ttgcttttgg taatatttta ccgtttctcc acatcgatca agagactgta
     4621 ctgacctatt tacgagaagt gattcctgag accgtttacc agtttatcgg tcctgcaatc
     4681 aaagaccttt tgacacaaag ttcaggaggc ttgttatccg tttctgcatt agcggcttta
     4741 tggtctgcaa gtcaaagtat caatgctttg caaattgcga tgaataaagc gtatggcgta
     4801 gaaaatagaa aaaatttttt gatcgtccgt ttcttctcac taattgtcat tgtcttgttc
     4861 atgattgcta ttagtggggt aactatcgtt cttgggttgg gacagctgat tttggatgct
     4921 attcagccaa tcgtacaaat acccgtgaac tttatcgatc agttccaaac tttgaagtgg
     4981 ccaatcacat tggtagcttt atttgtcatc atgttcttga tttatttaat tgtccctaat
     5041 gctcagttga agttgaagac gatcattccg ggcacgatct tcgctactgt cggctggatg
     5101 ctgttatcac aagtgtttag tatctatgct aggtattttg caacgaggat cagcggttat
     5161 cagatcatag gaagttttat tgtactcatg ctttggctta attttgctgc tacgatcatc
     5221 attttgggcg gaatcatcaa tgcagttgtt caagaatacg tgacgggtca tcaaatcaaa
     5281 aaacgtcgag atccaagccg aaaatggttc aataaattga aaactaaatt ttctcataaa
     5341 aaaaagtaga aacaagagtt cttattgtaa atttatatca gtaatttata ataattatga
     5401 ctcttaatta aagtaggtgc ccatatgctt gtttctttta gtatggttat aagatttttt
     5461 ctgacaattt tattatcact gatattaaca cctgtattca aaatcatttc agtacaaaca
     5521 ggtatggtcg acaaaccaaa tgaaagaaga ataaataaag ttccaatgcc ttcagcgggc
     5581 ggactgccta tctttatttc atttgttata tcgactttat tcttattccg taaaattatc
     5641 cctcaatcgt atattgtacc gatcttgatt gcttctttag taatcatagt cacaggagtg
     5701 ctcgatgata aatatgagct gacacctaaa caaaaaagta tagggatctt gattgcttct
     5761 cttattattt attttgtagc agatatacgt attgattcct tcacgcttcc ttttattgga
     5821 tatatccaac taggctggct tagttttccc gtaacgattt tttggatctt cggtatcacg
     5881 aatgcggtca atctgattga cggtttggat gggctggcgg cagggatttc attgatcggt
     5941 ttaattacga taggaatcat tggatatttt ttcctccatg cttctacagt gtatattcca
     6001 gttgttattt tctgtcttgt agcaagtatt atcggttttt ttccttacaa tttttatccg
     6061 gcaaagattt atttaggcga tacaggagct ctattcctag ggtttatgat ggccgtcctt
     6121 tcactacaag ggctgaagaa cgtgactttt gtttcatcga tttctttgct gatcgtcatg
     6181 ggagtgccgg taacagacac gttttttgct atcattagga gaaaagcaaa ccgtgtttct
     6241 ttttctacag cagataaaaa acatcttcat caccgactaa tcagtttagg ctttactcat
     6301 aaaggtgctg tcctgacgat ttatgcgatg gctttgatgt tttctttcac agctatggtc
     6361 atgaattata ctggaagaat cggaacgatc gtattgatta ttgccatgtt gtttgctgca
     6421 attttattat ttgaattgat tggtctgatc aatgagaaac atcagtttat tttaaaaaca
     6481 ttgcgatttt tagggaataa agagtaccgt acgcaagtaa aacagaaata tttgaagaaa
     6541 tggaaccgtc atatagatga ataacaaatt aaaccagccg atccttatta gtattgtgac
     6601 ggcaaacagt gataaaattt ttcggacact agataatttt atttcaactg taaaagatga
     6661 aacagcagta aaaatccgaa tttttgacaa caattcactt cccgagtaca aaaaaagact
     6721 aacagagtat aggaaatatc cctacatcga tatctatttt catgatgaaa attccggatt
     6781 tggttatgga cataatcaca atctgatagc tagcgattct cgctatggca ttatttgtaa
     6841 cccagatatt ttagtggatg aagagacagt tacagcattg gtcttgttat tgaaagaaca
     6901 tccggaatgt gcgatgttag cacctaagat tcttaatgaa gatggaacaa ctcagcacct
     6961 catccgtgag cagctggctg tttttgatta cgttttgcgc tttgtgcctt tcaaatgggt
     7021 caaaaaaaag tttgacaaac gactggctgt gtttgaatgc cgaggattgc ctactgatgc
     7081 taatagttat gtacggatga tctcaggtag ttttatggta gctgatattg ataaatttaa
     7141 agagataaag gggtttgatg agcgtttctt catgtacttt gaagacaacg acttgtgttt
     7201 gagttttgaa aaagcaggtt acaagttatt gtacacaccc ttttatgcag ttatccattt
     7261 ttacgggaag ggtgcgcatc gaaatgcaaa actgtttcgc gtgtttttga cttctatgat
     7321 gaaatttttc aataagtggg ggtggcgttt tttctgatga aaccagaaat attagtagtc
     7381 gttgtgcttt atcagcaact attcagtcaa tctccagctt atggaccatt aagcaaagca
     7441 cttagcgaaa agcaaattca gcttattatt tacgataata gtcctcaaag gcagcaaaat
     7501 gagctgtttg aaaaagaaaa tatcgtttat catcatgatc cgcaaaatcc tggtttagcg
     7561 gtggcttata attttgcgtt gtctcaagca gatgaaggaa cacgttgtct cgtcactctg
     7621 gatcaggata cgaggctagt cgacgattat tttgaactat tgcgtaaagt aacctttaca
     7681 gatgaatgtg ttgctgcagt tccgatgatt ttttctggga gccgacaagt ttctccccta
     7741 tattcagatc gttatatcaa tcgtacagct caagcagtag aagttgaagg cccaacagct
     7801 gaacgaatca tggcaatcaa ttcaggtgtt gcctggtctg tcaaatttct aaaaaagatt
     7861 ggcgggttta atcctgcttt ttctttagat tttttggatc attggctgtt ttggaaagtg
     7921 gatcagttgg aaaaaactgt ggagatcctt cctgtacgaa tggaacatga tctttcggtt
     7981 ttagactatg aaaaagtttc aagtaatcga taccgttcta ttttacaagc agaaaatcga
     8041 ttttatcatg aatatgatca aaggcattta tccagtcacc gacgacaatt attgttaaga
     8101 acagctaaac agttcttgac cgtcaaaaac cggcatatct ggcggatgac tttgcgctca
     8161 tatgttaaga attggaaggt gtgaattcgt gcgttcggtt tgtatagcta cctttaatgg
     8221 aggcagatat ttaagagagc agttagatag tattttatct cagctttcag cagaagatga
     8281 agtagtcatt tctgatgatg ggtcagtaga tgatacatgg gatatattaa ctgaatacgc
     8341 agaaaaagat aagcgaatcc agcttttttc ggggcctcgt caaggattga tcgctaattt
     8401 tggttatgcg atccagcaga cgaaaggaga tctaatcttt ttggcagacc aagatgatgt
     8461 ttggttggag acaaaagtga aaaagatcac tgactatttt gagcagaatc cggatcagct
     8521 agtagtagtt tctgatttaa tcattgtgga cagcgagctg caagagatcc atgattcgta
     8581 tttttcgttt cgaaattcca aagaaggatg gcttaataat gtgataagaa gtcactatat
     8641 tggtgctggt atggctttcc ggtcttcttt aaaacccgat attttgccga tcccgtcaga
     8701 agtgccgatg catgatatgt ggattggttt gctgggagga aagaagactg ggttcctaag
     8761 agaaccgtta actctgtacc gaagacatga tttaaacgcg agtgagattg aaacacattc
     8821 taaattacgg caaaaaattt cttggcgtgt tcatctgatt acggcgttga tcaaacgaaa
     8881 atggtttaaa cgttgataaa agatgtttaa atccttaata tttccatgac aaatataaca
     8941 attgatgaga taataacaaa gtgaaatttg aaatcaaaaa ggagagcaga tatccatgaa
     9001 gggaatcatt cttgctgggg gcagcggaac acgtctgtac cctttaacaa aggcaacatc
     9061 gaaacaattg atgccgattt atgacaaacc aatgatttat tatccaatgt ctactctgat
     9121 gttggcggga atcaaagaaa ttctgattat ttcaacgcca caagatacac cacgtttcaa
     9181 ggaattattt ggaaacggaa atgatttagg actgcagatc gaatatgcag tacaagaaag
     9241 tccagatgga ttggcacaag cgtttatcat tggagaagaa tttattggag atgatagtgt
     9301 ctgcttggta ttaggggaca atatttacta tggtggcggt ctgtctaaaa tgttgcaacg
     9361 agcagcttca aaagatacag gcgcgacagt atttggctat catgtcaatg atccagaacg
     9421 ctttggtgta gtagaatttg atgagaatat gcaagctttg tcgattgaag aaaagcctgc
     9481 tcagccaaaa tcaaattacg ctgtaactgg cctttatttt tatgacaatg aggtaatttc
     9541 tatcgctaaa ggtatcaaac cttcagaacg tggagaacta gagattacgg atgtcaataa
     9601 agcatatttg gataaaggaa aactatccgt agaacttatg ggacgcggat ttgcttggct
     9661 tgatacagga acacatgaat cattgcttga agcatctacc tttatcgaaa caattgaaaa
     9721 acgtcagaac ttaaaagtgg cttgtttgga agaaattgct taccgtatgg gctacatcga
     9781 taaggaacaa ttgctggccc ttgcacagcc attgaaaaaa aatcaatacg gtcaatattt
     9841 attgaatttg gccgctgaat aaggagtcct tacagatgaa agtaacagat acaaaattaa
     9901 cagatgtcaa aatcgtagaa atggacgtct ttggcgatca tcgcggtttc ttcacagaaa
     9961 gttattcaaa agcaaaattt gcagaacatg gattgaaata tgactttgtt caagacaacc
    10021 attcactatc agctgaagct ggtgtccttc gggggcttca tttccaaaaa ggagaagctg
    10081 cacagacaaa attgattcgg gtagtcactg gagcagtttt agatgtagta gtggatatcc
    10141 gcaaaggtag cccgacttat ggtcaatggg acggatttat cttatctgaa cataatcatc
    10201 gacagttgct tgtacctaaa ggatatgccc atggattcgt tactttgaca ccaaatgtga
    10261 acttccttta taagtgtgat aattactata atgcagaagc agatgggggc atcgcatttg
    10321 atgatccaga tttagcgatt gattggccaa ttgatccatc aagagcgatt atgtcggaaa
    10381 aagatcaccg tcatccgaca ttaaaagaat ttgaagcaga aaatccattt gtttacggag
    10441 aaatctaaga attaaggaga aattttctta tgaaaaacat cattgttacc ggaggtgccg
    10501 gattcatcgg ctctaatttc gttcattacg tggtaaacaa tcatccagaa gtgcatgtca
    10561 ctgttttgga taaactgact tatgcaggaa acaaagagaa cttggccgga cttcctagcg
    10621 atcgcgtaga attggtcgta ggagatgttg cagatgcaga attagtggac cgtctagtaa
    10681 aagaagcgga cgcagttgtt cattacgcgg cagaatccca taacgataat tcattaaaag
    10741 atcctttccc atttgtacaa acgaatatca ttggtacgta tacactttta gaagcttgcc
    10801 gcaagtatga tgtacgctat catcacgtat ctactgacga ggtttacggc gatcttccat
    10861 tacgtgaaga cctgccagga catggggaag gcgaaggaga aaaattcact gcggaaacac
    10921 catataatcc ttcaagtcca tattcttcta caaaagctgg ttcagatctg ttagtaaaag
    10981 catgggtgcg ttcttttggc ttacgtgcaa ccatttcaaa ctgttcaaac aactatggtc
    11041 cttatcagca tattgaaaaa tttattcctc gccaagtaac gagtatctta agcggtattc
    11101 gtccaaaatt atatggcgaa ggaaagaatg ttcgtgactg gatccatacg aatgatcatt
    11161 cttcagcagt gtggctgatt ttgacgaaag gcagaattgg cgaaacctat ctgatcggtg
    11221 cagacggtga agaagataat aagaccgtga tggaattgat cttagaaatg atgggccaac
    11281 caaaagacgc atacgaccat gtaaacgacc gcgcagggca cgatttgcgt tatgcaatcg
    11341 attcgacaaa attgcgagaa gagctaggtt ggcagcctga gtttacgaac ttccgtgatg
    11401 ggttggctga cacgatcaaa tggtacgaag aacatgaaga ctggtggaaa aaagaaaaag
    11461 aggccgttga agcagcatat gccaaaaatg gacaataata cagaggaaat ttcactgtat
    11521 taatggaaaa acacatgtga ggcaggcgct cgcctgtctc ttttttaaaa taaaaatcaa
    11581 ggagcggata accatatgtt tttattaact ggaggaaatg gacaattagg tacagaattg
    11641 cgccatttat tagatgaaaa aaatctgcca tatgtttcaa cagatgcaaa agacttggat
    11701 atcacagatg ctgagaaaac aatggcttat attacagaat taaaacccga tgttattttc
    11761 cattgcgcag cttacacagc agtagataaa gcagaagaag aagcaaaaga attagatgag
    11821 aaaatcaatg tcgatggcac aagaaatgta gcacttgcag caaaagctgt gaatgcacaa
    11881 ttcgtctata tcagtacaga ctatgttttc gatggaaaaa acaaaaatgt agaatacgaa
    11941 gtagacgacc aaacgaatcc actaaatgaa tatggtcgta cgaaattatt aggcgaaaaa
    12001 gcagtagaag agattttaga agactattat atcatacgaa cttcttgggt tttcgggatc
    12061 tatggacaca atttcgtctt cacgatgcaa cgcctagcgg aaacccgcga caaactgact
    12121 gtagtaaatg accagtttgg gcgaccaact tggacacgta ctttggcaga atttatggta
    12181 tatgtcatcg aagaaaaagc accatttggt atttatcatt tgtcgaacga aaatagctgt
    12241 tcttggtatg agttcgcaaa agaaatattg aaagatacag atgtagaagt tgcacctgtt
    12301 acgtcagaag aatttccgca aaaagcaaca cgtccgcagt attcagtcat gagcctgaaa
    12361 aagacagaag cgttaggttt tgtcatacca acttggcaag aagcacttgc gcaaatgctg
    12421 gaagctgcca aaaaaatgaa taagtaattt cctttttatc aaatagaatt tggagcatga
    12481 tatggaaaaa caaacaagaa gtgcacgacg aaaagaaaag ccaaaagcga atcaggcaga
    12541 ttcgaatttg gtgcttgacc tgtttctaaa actccgctat cttattggcg ttttagtcat
    12601 cgtggtcatc gttactttta atttaaacgg aagttcactt ggtgtttggg ataaatatgt
    12661 gtcgcaaaga gacgatggga aaaaaactga tgtgattttt ggacagaatc gggaagtgcg
    12721 gtcagacgaa tggctggtgc agacaccttt ttacctctca caagctgaag aggacttttc
    12781 gttggtgaac gaggattatt cattaaacgg acaaaacatg atcattgcgt ataattctcc
    12841 tgtaaaggat attacggtta tcggaaaacc attcaactgg ggattctttt tcctcggaag
    12901 agatcgagga ctttcctttt attgggcgat gaaattagtc gtcatggtct tgcttggatt
    12961 cgaagtactg atgatattaa cgaagaggaa aaaagctcta tcgctaatcg gtgcatttgc
    13021 gctgacattt tctccagcag tccagtggtg gttcatgcaa catgtgggcg atttgatttt
    13081 ctttactttg ggactgatga ttgcttttta tcattatttc tacaatcatg ataaaaaatg
    13141 gctacgaacg ttgatgatgc ttttggtggt tattttcggt attgggttta ttttagtgat
    13201 ttacccagcc catcaagtga tgctggcgta cttgctgatt ttttattttg ttggcctgct
    13261 tatttactat agcaagaaag tttcatggga tctgttggat gtagtgttga ttggaggggc
    13321 agttttattt attggcggag tcatggttca tttctggcta acgtcaaaag acgcattgtt
    13381 agcttctttg aataccttat atccaggaaa acgggtaagt actggaggaa agtggacgat
    13441 tggcgaattt ttttctttcc ttacaaattg gaaaattcct tttgaagata tcaactattc
    13501 gaataactct gaagtggccc tgttctatca cttttttcca actgtattcc tagcaagtcc
    13561 atttgtgtta tttggcaaaa aagatagcga acaaaagtta ctgggacgta ttttgatgat
    13621 tttttgtctg ttttctatct tttggattac cgttgggttg ccaaaatggc tagcagaaat
    13681 cactctgctg tcttatgttc ctccattaag agccatcctt acttttagtt ttgcggcttg
    13741 tttattatcg atctggttta tcgcttatat ttggagagaa gatgttattc ctatttggta
    13801 taaattcatt ttgttggcag taaatgcatg gtcttatttc tatgcgatta cgcattcgaa
    13861 aatggataat tatttttcgg attttgaaac catcgcagtt gctgtgttgg gaacagtgat
    13921 tttagctttt gcattatttg gctggagacg cttgttttac ctcgcttttg tcgccttagt
    13981 tatcgtatct ggctttttcg tcaatccagt cgtagaaggt acaggagcga tttttgaaaa
    14041 aacactggca aaagaaatcc aaaaaatcga tcaacaagat cctggtaaag tttggttgtc
    14101 tgaagatgaa ttatataact tcaccccaac attgggagtg aaatccttca atacagtacg
    14161 cttttatcca gatatagctg catggaagaa aatcgatccg aaagaagaat acgagaagta
    14221 ttataatcgg tatgctcata caagagcttt tatcgctaag gaagatacaa agttcatttt
    14281 agatcgtcca gatatgtttt cggtcacgtt gaattttgaa gacgcagaaa aattagggat
    14341 cgactatgtg atcagtaaac gaccattaac tgattacaac caattatatg aagcgcagtt
    14401 cacgcagcta tatggaccgg ataaagacgg ttatatgatt tttaaagtag aatatccgga
    14461 ttatccagat gtaccacaaa cacaaactca ggaaaatcag ctagtcgtac agtaataggc
    14521 tagctgagaa taatcctgaa atattagtag aaaggagagt gaagataatt caagcaatcg
    14581 tacaaaaatt tgtcaacaaa atgagtgtcg gtttcttgct tttgttgctg acgctagtct
    14641 tttttgtttg gacagcaggg gtaggttttc cagcaacttt gtttatatat aacaatagtt
    14701 cattacttat cgcttttagt gtggctattt tgctcatttt aaatgtttat aaagcaaaag
    14761 gcgtcgattt tctttatcta ggtgttgcgc ttgccctttt cattttcttt tcttactttc
    14821 gggaattaag aaatgagtct ttatttggag acttactgtt tcctttggtt attttatttt
    14881 gtttagtaat taaatggatc gagtggacaa aatgggatcg cggtatttat ctgattgtct
    14941 ttacggtctt cctcggaatc acgttatatc gtgtatttac agagatccaa gtaccagaag
    15001 gacaaagcat ctgggctcca ggcaattcgc tttctgatat ttggatcaat acgaatacaa
    15061 tcggaagttc tttgatgact cttgctttat tgatcagtgg atttgccagt tcttttgaac
    15121 gatggtatgt tcgaatcatc ggtgggatag cagtcgttgc agccttttta ggtatttggg
    15181 tctgccaatc tagaggtgca ttagtcgcgt tgattgtttt tgcgatattg gatcttttac
    15241 ctaaatcgtt gatgcgtgtc attcgtgcgc catttattgc ctacacggtt acgattcttt
    15301 tagctttgcc gatttcttat ctggcggctg tctctgaaaa agtcaatttg tttaccgatc
    15361 gagaagatat ttggcataaa ttctatcaga cgctaggaga aaaatctgaa caagttttac
    15421 taggaatgaa aacatttatc ttccagcgag gaaaccaatt tttaggaaac cacaatagtt
    15481 ataattctat tttgaatatc tatggattga taggatttgg gattgctgct ttgctgctta
    15541 tcctttttat tggtcgctta acgttgaaag cagatttatc caacgggcag atgactttta
    15601 tctgggcctt ttttgcggtg atgatgcagt catttatgga ggatacattg acttcatacc
    15661 catgggttcc gattatttat atcctgttgg ctatggccgt tcatcgatat gatgaaccag
    15721 aagttgcgga ggaattctat gttatggaag aggcatatga cgatatgact tatgatgata
    15781 catcttatga ggaacaacca gatagcgctc attcttccag gatccaacgc tatcattgat
    15841 aggcacaaaa tacgagagag gctgtgacaa ccgtcgcagc cttttttggt ttattgcaat
    15901 tgtctagcat cttctgtaaa atagaaacgt tagagaaaat cttgtatcat agaaaattaa
    15961 gaaagaaata agaacgcagc tcggtaccaa aaatatggta aaatggcact tgactgtaat
    16021 tcactgaaaa tcgcttggag gaacacaaat gtttaaagat ttgtatttat ttatgaagga
    16081 catttatcag aacagaaaat tattgctaca gttttcgatc aatgacttca aatcaagata
    16141 tgcaggatcg tttttaggaa tcttatgggc gtttatcaat ccgctattta ctgtattgat
    16201 ttattggctg gtatttggtg ttggactgaa agcacaaatg atcgatgcaa aatacccatt
    16261 tatcgtatat ttagttaccg ggatgattcc ctggttcttc ttctcagatg catttgcatc
    16321 aactacggtt gtttttcggg aatatactta tttagtaaag aaagtcgtat tcaatattcg
    16381 tatcttgccc acgacgaaaa tcctatccaa tctgtacacc catttattct tcattctgat
    16441 tggcatagct gtcgcgctta taaatggtat atatccttca gcaatgtctt tacagctgat
    16501 ttattattta ttttgcatgt gtgctttctt gacaggattg acttggctga cggcatcgat
    16561 ccaaccattt ttacctgata tcatgcaatt aattacgatc gtgctgcagt tgatcatgtg
    16621 gacattaccg atattatgga gccctaatca gtttgattca ggtcttgtaa gcatattgaa
    16681 attaaatccg ctgtactatg ttgttcaagg ttatcgtgag tcatttttaa gtgaagcttg
    16741 gttctgggag catggaacat atactttgta tttctgggtt ttcacgttag taatgttcgt
    16801 aatcggctct gcagtgttcc gtagattgaa accacatttt tcagatgtgc tgtaaggaga
    16861 atagctatga cagaatatgc aattagacta aaagatatca caaaatctta taatatgtac
    16921 gcaaaacctt cggatcgatt tcgtgaagcc ctcaatcctt ttaagaaatc ctatcatgat
    16981 gtattttatg cgttgaaaaa cgtgaatgta gaaataaaaa aaggtgagat gatcggtttc
    17041 gtgggagaaa acggatcggg aaaatctaca atgttgaaaa tcattacggg agtactgaca
    17101 ccaacatccg gcacgatgga aatcgaagga aaaatctcag ctttgctaga actaggatca
    17161 ggattcaatc ctgagtattc tggctatgaa aatatctatt taaatggaat ggttctagga
    17221 ttttcaagag aagaagtcga tgaaatggtc gatgatatca ttgaatttgc tgacatcgga
    17281 gaccatattt atcaacctgt aaaaacttat tcaaacggga tgttcgttcg tctagctttt
    17341 gctgtagcaa tcaatgtgaa tcccgatatt ttgattgtgg atgaagcatt ggcagtaggc
    17401 gatcttgaat ttcaactaaa atgtatggaa aaatttacag aaatcaagaa ctcgggaaag
    17461 acgattttat tcgtttctca tgatatcagt tcgattcgtc gtttttgtga tcgttcctat
    17521 tggttgaaaa acggagaagt cgtcgaatat ggcgatacga tggaagtaac gacgaattat
    17581 gaaaactttt taaagaaaaa atcagttaaa acagtggatc ggaataaaaa ccaagtagaa
    17641 cggttcgccg ctccagatat cgtcgacgtg acgaaagcgg aattgttgga taaagacaaa
    17701 aatccggtgg atatgctgga acaaggagaa aaactatacg ttaaagtaac ttatgaagta
    17761 aaagatgata caatcaaaaa tccggtttta ggaattgcgc tacgaacagt cgacaatttt
    17821 tatgtatgcg gacttaatac tctactagat ggtatcaaga ttccttggaa aaaaggagag
    17881 aatatctttt atctggagta tgaaaagtta ggtttgttag ctggtgaata ttattttgac
    17941 gttgcggtct ttgaagaaaa tgcaacggtt cctttggtat ataagacaaa gtacatgaac
    18001 ttgtttgtca gcggttctta tatcggagaa ggcatcgtgg tcttagacca taaatgggaa
    18061 gaagggactc atagcaatga aatatgattt tgaaatggac ctagatgaac aaagcagtgt
    18121 gggaaaaatt gccgcacaga tcaaaccagg aagtaaagtt ttagagtttg gtcccggaaa
    18181 cggccgatta acgaaacatt taatcggagc aaaacaatgt gaagtgagca ttgttgagtt
    18241 agataaggaa ttgttcgatt ttgtcagcga atttgctcaa gatggatttt atggtgatat
    18301 cgaaagtttt gagtgggcaa actattatgc tggacagaca tttgattacg tcttgtttgc
    18361 ggatgtttta gaacatttgg tagatcctgg aaaaacattg aagaaagtca gagaattttt
    18421 gaatgaagaa ggagagatcc tgattacatt ccctaacttg gcgcataatt ctgtcatgat
    18481 cgatttgttc aataaccaat tgccgtgggc ttcttatggt ctcctagatg aaacccataa
    18541 ttctttttat actcatgatg gcttccaaaa agtttttgaa aaagcgggat tattcattaa
    18601 tattgaagat tatttgtact tagctgtagg tgatacagaa ttaaaatcaa cttatgaaga
    18661 attgccggaa gctgtacgct atgatttcaa aatgcgacca tttggtgaag tctaccaata
    18721 ctttttctca ttgatgaaac atccagtagc acaaagttcc attgcagagc cacaaaattc
    18781 taattatgta aaagtgttag aagtaaccca gcaaacgaaa caagatgaaa caatccaaca
    18841 aataccattc aataatttca caggggaaaa tgagacacta agtttccccg tatcagaaac
    18901 aacagaaaga atggtgtttc gcttcgcgca acaacctagc tttatcgaat tcagcgcaga
    18961 agctgccgga gaaaaactaa catttatcga ttcgaatgct gtagtcaaaa cagtaaatga
    19021 ttgttatttg tttgatggga aagaattacc agagttcgtt ctaacggata tttctgggaa
    19081 agaagtgact atccattgtc actatcgctt tatcggggaa ttgacaccaa caatgaaaga
    19141 attgttggaa acgattcgtc ccatggctga agtcacaaag cagctttctg agaaaaatga
    19201 cgaattagaa agagaaaatc atcatcttct tgaggaaaat acaagactag accatacgtt
    19261 aaaaacaacc acaaatcgct attgcacatt gttggaaagt gatgaatgga caataaaaca
    19321 tagactagga cgacgtaaga aagaaacttc gaaaaaaatc caagaaaaag aactttctat
    19381 atgtattgac gagaaaatct gggatgcaga aacaaaaata ttaaaaatca tcggatgggg
    19441 aatcgctaat tcggatcgcc agcccttgtc ctataagtta tcagctgacc aatcgccatt
    19501 tttcgaagca attcctgttt cacgagaaga agtcaatcaa gcagaacagc tcgcaaaagg
    19561 aacagaagcg ggatttgagt tacgaatcct ttgcgagcaa gaacgttcat tcttagtgga
    19621 agcagtagct caaaatggcc aatcatggat cattgaaatg taaataatga ttaacttata
    19681 aagataagga gtaaacgaat cgtgcaaaaa aaagaaattg ccgtatttat cgatcaaatc
    19741 actcgtgaac gggccactgg cgatttgctt attgtgggct gggcaattga tgaagtgacc
    19801 aaagaaatcc ctacgataaa ggtagaaaaa gaaaatgtga ttgctgaagc aacacatgtc
    19861 gtaagattgg atatcaatca tttatataat cttgatgtta agacccaaag cggtttcaag
    19921 gtacgtttat cagggaaaat gagaggaaaa gccatcttag acttccaaac agcgagacat
    19981 caaaatggca tagctgtgaa attaaacggt aaataccctt atgatgacgg cattgaatct
    20041 tcatgggaaa gaaaaaaaag attactgaaa aaagggataa attatgcaag gactcatggc
    20101 gtaaaaaaag cgatccgtcg ggtcaaattg gaattgaagc caggatcgat cgactatgca
    20161 gagtggatca gtcgtcatga gaaaccagat ttgaaagagc aaaggaaaga agcacaagga
    20221 tttgcgtacc gtccattgat ttccatactt atgcctgtct ataacgttga aatcaaatgg
    20281 ctcgaaaaat gcattgattc ggtactggat caaacgtatg atcattggga actatgtatc
    20341 agtgatgatg cttctactga tccagctatc cgaaagtgtc tggaatccta tcaagcaaaa
    20401 gatgatagaa tcaaagtcgt tttccgaaaa gaaaatggac acatcagtct ggcaactaat
    20461 tctgcattgg aaatagcaga aggagaattt atagcactct tagataatga tgatgaatta
    20521 ccgccatttg cattatatga agtggcaaaa gtgctaaacg tacatccaga actggatgtg
    20581 atctacagtg acgaagataa aatcgatgca gatggcaatc gctttgatcc gcatttcaaa
    20641 gcagactggt cgcctgatac attgatggga aataattata tctctcactt aggcgtatat
    20701 cgaacaagca ttgttaaaga acttggcggt ttccgtaaag gctatgaagg ttctcaagat
    20761 tatgatttgg tccttcgtgt gacagaacaa atccctgcag atcatattta ccacattgac
    20821 cgagtcttgt atcattggcg gacgatccca ggatcaactg caagtaatgg cgaagccaaa
    20881 agttatatct atgattccgg agtgaaagca ttgacagatg ccctgtctag aagaaacatc
    20941 aaaggaagcg tacgtcctgg cagaatttct ggattttatg agattgctta tgacgtcttg
    21001 caagaggatt tggtcagtgt gatcattcca acgaagaatg gctatgaaga tctaaaaact
    21061 tgtgtggatt cgatcatcga aaaaacgtct tattcaaatt atgaaatcat tattgcggat
    21121 aacggaagta cagatccaaa aatgcaagaa ttatttgcag aatacaagca tcagctgaaa
    21181 gaccgcttta tcgtagaatt gattgacatt ccattcaatt attctcgaat taacaattta
    21241 gcagcagaaa aagcaaacgg caaatacttt ttattcttga ataacgatac agaagtgatt
    21301 gagccagatt ggatgacggt gatggtttct tatgcacagt ttgatcgaat cggctgtgta
    21361 ggagcaaaac tgttttatcc agatgataca acgcagcatg caggagtatt attaggaatc
    21421 ggtggtgttg cagggcatgc attgaataac tatgatcgaa cacattgtgg ttatttcggc
    21481 cgattggtca ttgacgtcaa ttacttagca gtcactgcag cttgtatgat ggtcaaggca
    21541 gctgacttca atgctgttaa cgggtttgat gaaacattag aagtagcatt caacgatgtt
    21601 gacttgtgct tgaaagttta tgaactgggt cgttacaatg tctatgcaca tcaagcagaa
    21661 ttgtaccatt tcgaatctaa atcaagaggt tatgaagata ctccagaaaa acaaagacgt
    21721 tttgctggtg aaatcaaaaa aatgcaagat aaatggccag catatattgc gcatgatcca
    21781 ttttacaatg ataatttaac aaaagacgga atcggcgact tttcgttgag accagattaa
    21841 actataataa aaagctgtta tgttgtgatt cgctcgcaca taacggcttt ttatagtttt
    21901 ttgtcaaaaa aaggcagaag gtgccgactc aaaaaaataa gtcgaggttg ggacagaagt
    21961 gtttaactcc gagaaataag aagaaattca cgaaaattgc ttttcaaatt tttgtgaatt
    22021 tcggcttatt taagaagaag ttgcttctgc tcccaccgtt tattcgtttt taaagaatga
    22081 gacaaaagtg tttcttactt ttgtcccacg ctcttattca tgctcagagc gccaatagat
    22141 acaaaatcat agccaaagtt tcacgaaccc tagctgtcaa agaatcgata ttttatttta
    22201 atataggcta ttttaaaata aatggtttaa ggtattcctc tacaaaagcc actctatcaa
    22261 ttatcatggg tttaaaataa gaagtgatgg agataaccag attgttctta gcgtcaatat
    22321 aaagaacatt tccactatta ccaattgctg aatatatttc gttctcagaa tctaataccc
    22381 accataaata gccatatctc atattttgta agtcttctaa agcactggtt ctaggtttga
    22441 acatctcgtt tatccacatg ggtggtacga tttgttttcc tttatacatc cctttattta
    22501 ggcataatac accaattttt gccagatcac ttgccgataa acacaaacca tatcctgccg
    22561 tcccgatatt tagtggatca caaaaccaga cattggtttt aggagactta tccattgtaa
    22621 actgcttatg ttcttcagct gtttcagcat aataattgac atggtttggt atccctatag
    22681 gatcaaatag atacttattg gcaaaatcaa cagttgttag cttacttgtc tgatacaaca
    22741 agccagttaa tatgtgtaaa cagacagttt gatagttgaa ttcgccggtt ataccttttc
    22801 ttcctcccaa taaatctagt gatgtttttg tccaatcttc acttgaacag acttttgtcc
    22861 atggatcgcc tttacatttg tagggtgctt tcattgttag caagtgcttg attgtaatag
    22921 cgtaaatagt tttctccccg cgtttcactt tatactctgg aaaataatct aatactttat
    22981 catcgatact tttgattaat ccttgctcta aagcaatgcc aatcagcaaa gatacaatac
    23041 ttttcgttgc tgacatgata tgacaagtat cgtctttttt ataaccattc cattcatctg
    23101 tataaacttc taccccatcc ttatatgcga caatttgaca tatatttggt tgtcgaaaac
    23161 caataaaatc atgcaactct tgtttattca taagatatta cctcactttc ttctataagc
    23221 ataatgtcat taaaaatcga tttcaatttt ttcgattctt cacgtaatac aggtattctt
    23281 atggactaga cagcgctttg ggaagatgga ttctctgaaa aatataaagg ttcactttat
    23341 gagtggataa aaaaagcagc aaatacttcg acttctttaa tttaccaaca ttagtgaaaa
    23401 tcgattcatc tggagatttg taatttagag tccttgtctt gattagaggt gtataaagct
    23461 ttttattaga catttttaaa ttccattaca atttgcttat aaactaggtt tttaaaggtg
    23521 actatggtaa gataatagag atattatttt gtgattagga gtgtatatat gaataaaaat
    23581 ggagaatgga gtgctgcacg acggatattc attatattaa tggatgttat tgtatacaat
    23641 gcagcaatat atagtagttt cttactgaag tttcgtggag agatcccatt acgaaattta
    23701 gaaactttcc aacactcagc aattattatt tcagtgattt ttattgcgtt gaacattttg
    23761 ttgggtgcat atgtttttta taatcgaatg atcagtgaca tcgtgtttgt cacagtcatc
    23821 ggtcaggtat tgatgacact agggatcatg gtagtgactt ttgcgggccg ctggtttgct
    23881 tttccacgtc ttgttatctt actttcattt gttctcggta cgcttttctt aagtatctat
    23941 cgtattctcg tatataaact ttatttacgc gtaagtcgag ataaaaaggt cgtcattatt
    24001 gggttagaaa aggatgtcgc accagcaatc caaaattttg gttcaaagaa aaacaacaaa
    24061 cataaagtcg aagcagtcat tcttgataac tattatgcaa acatcaaaaa tatgattaaa
    24121 gacatcgata ttgtctatct tgcttctcat atcgaagaaa ctgaaaaaat caaaatctat
    24181 caattagtta ctaaaaatga aaaaaaattg tttttgaata caacatttga aaacttgaca
    24241 ttgatcaatc caaatattat gaatttcgaa gatgaaagta ttattgaagc atctggattt
    24301 agaatccaac ctgaatatga actattcaaa cgtttgtttg atatcatcgt ttcacttgtt
    24361 ttattagtta ttgcttcacc atttatgtta ttcacagcaa ttttagtcaa agtgacatca
    24421 ccgggaccaa tcatctataa acaagtgcgg atcacaaaaa atcaaaagga attttcaatc
    24481 tataaattcc gctcgatgac cgccacagcc gaagcaaaat ctggaccagt tttagctaag
    24541 agcaatgatg cacgagtaac gcctgtaggt aaatttattc gagctgtccg ctttgatgaa
    24601 ttaccacaaa tcttcaatgt gctgaaaggc gatatgtcga tcgtaggacc acgtcctgaa
    24661 cgttctttct tcgtcgatca gttcaatgca gaaaaccctt attactactt acggcataat
    24721 gttcgtgctg gtatcactgg gtatgcacaa gtctatggaa aatatgtctc tgattacaac
    24781 agtaaattac gttttgattt actttatatc aaaaaatatt cattgatgct tgatttaaag
    24841 atcttgctgc aaaccatcaa gatcctattt gataaaatgt cctctcaagg acttgaagaa
    24901 gacgaaaatg tgatctcatt ggcagaaatt gcttttccga atgaaaaaat ctttcgctag
    24961 ttgggaggcg taaaaatgaa caatccgaaa ttcagcataa tcgtaccagt atacaatgca
    25021 gaagcaacta ttggtcgaac gattgatatt ctaaaagaat tggattatga agagtacgaa
    25081 gtcgttttag taaatgatgg atcaacagat caaacacagg aaatcattca agaactgatt
    25141 gaaaatgatg aacgttttga attgattact acgaccaatc aagggcccgg attagcaaga
    25201 aacgaaggaa taagacagtc aaaaggagaa tatctcttat tcttcgatgc agatgacacg
    25261 ccagtaaaaa cgatcttgtc agattatgat catttgctag aaaagagtcc aaaagcggat
    25321 cttgttgtta gttcgtttgt ttttcgtaca atggacgagg gaagaatcgt ttccgaaaaa
    25381 gaaaacttag ttacagaatg tcactatcta actcacgaag agtttataaa agacatgtat
    25441 cagttaatga atgaacagtt gatgtatgtt gtatggaaca aatgctatcg tagcgatatt
    25501 ctgaaaaata accaaattct atttaaaact tataatagtt gtgaggatcg aatatttaac
    25561 ttacattatt atgaatactg tcatcaagtg atcatgaacc caaaagtaga atatatttat
    25621 gaatttgaag gtgggaaggg gatcactaat caataccgac ctaacaagtt cgaaacattt
    25681 aaagaattct attcattagc aaatactgta acagacgata ttaataaaga cggtatggcg
    25741 gcgcttcttt taaaagggac aacttcagtt attttttcta tttatagtac ctctgcaaga
    25801 acttcaaaag agaagaaaga cgaagcaaag cagatattga gtgatccagt gatccgagaa
    25861 gccaagcaaa ttgctaagac ggattcttca gcaaaaaaaa taacaaagtt gctttacaat
    25921 atgccagctc cactattttt atctgctgta aaagttggaa gcttcgttga agtgaaaatg
    25981 ccagggttaa tggctgcttt gaagcgagta tattagttaa tggaggaaaa tataatgaaa
    26041 agaattttgc attttcaggg acgaatgggt ttaggtggag cagagtcttt catgatgaat
    26101 ttatatcgta aaattgatcg tacaaactat caatttgatt ttttgattta tgaagattat
    26161 gcagatgttc aagactatca ttcagagatt gaaagattag gaggccgtat ttttgttgta
    26221 cctaatccta agaaaaatat tttta
//
LOCUS       DAYYFU010000033        25502 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000033 DAYYFU010000000
VERSION     DAYYFU010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.033"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACHA34_001654"
     CDS             133..3660
                     /locus_tag="ACHA34_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM8792274.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACHA34_001655"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACHA34_001655"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFM8792275.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACHA34_001656"
     CDS             7448..7789
                     /locus_tag="ACHA34_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8792276.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACHA34_001657"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACHA34_001657"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFM8792277.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACHA34_001658"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACHA34_001658"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFM8792278.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACHA34_001659"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACHA34_001659"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFM8792279.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACHA34_001660"
     CDS             13000..13737
                     /locus_tag="ACHA34_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8792280.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACHA34_001661"
     CDS             13751..14566
                     /locus_tag="ACHA34_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8792281.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACHA34_001662"
     CDS             14579..15226
                     /locus_tag="ACHA34_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8792282.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACHA34_001663"
     CDS             15242..15898
                     /locus_tag="ACHA34_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8792283.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACHA34_001664"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACHA34_001664"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFM8792284.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACHA34_001665"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACHA34_001665"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFM8792285.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACHA34_001666"
     CDS             18240..18758
                     /locus_tag="ACHA34_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8792286.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACHA34_001667"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACHA34_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFM8792287.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="ACHA34_001668"
     CDS             complement(19645..20565)
                     /locus_tag="ACHA34_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFM8792288.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20691..21743
                     /locus_tag="ACHA34_001669"
     CDS             20691..21743
                     /locus_tag="ACHA34_001669"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFM8792289.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACHA34_001670"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACHA34_001670"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFM8792290.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="ACHA34_001671"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="ACHA34_001671"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8792291.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="ACHA34_001672"
     CDS             complement(23383..24144)
                     /locus_tag="ACHA34_001672"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8792292.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="ACHA34_001673"
     CDS             24333..25427
                     /locus_tag="ACHA34_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFM8792293.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8448 a         4424 c         5384 g         7246 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 gg
//
LOCUS       DAYYFU010000034        25215 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000034 DAYYFU010000000
VERSION     DAYYFU010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.034"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..767
                     /locus_tag="ACHA34_001674"
     CDS             120..767
                     /locus_tag="ACHA34_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8792294.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    896..1112
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1160..3805
                     /locus_tag="ACHA34_001675"
     CDS             1160..3805
                     /locus_tag="ACHA34_001675"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFM8792295.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4115..5428
                     /locus_tag="ACHA34_001676"
     CDS             4115..5428
                     /locus_tag="ACHA34_001676"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFM8792296.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5418..6071
                     /locus_tag="ACHA34_001677"
     CDS             5418..6071
                     /locus_tag="ACHA34_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8792297.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6126..6677
                     /gene="radC"
                     /locus_tag="ACHA34_001678"
                     /pseudo
     CDS             6126..6677
                     /gene="radC"
                     /locus_tag="ACHA34_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            6808..7893
                     /locus_tag="ACHA34_001679"
     CDS             6808..7893
                     /locus_tag="ACHA34_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8792298.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7890..9782
                     /locus_tag="ACHA34_001680"
     CDS             7890..9782
                     /locus_tag="ACHA34_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFM8792299.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            9789..10166
                     /locus_tag="ACHA34_001681"
     CDS             9789..10166
                     /locus_tag="ACHA34_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HFM8792300.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            10320..11102
                     /locus_tag="ACHA34_001682"
     CDS             10320..11102
                     /locus_tag="ACHA34_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HFM8792301.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(11228..11959)
                     /gene="erm(A)"
                     /locus_tag="ACHA34_001683"
     CDS             complement(11228..11959)
                     /gene="erm(A)"
                     /locus_tag="ACHA34_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HFM8792302.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(12017..12076)
                     /locus_tag="ACHA34_001684"
     CDS             complement(12017..12076)
                     /locus_tag="ACHA34_001684"
                     /inference="COORDINATES: protein motif:HMM:NF019858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HFM8792303.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            12468..13130
                     /locus_tag="ACHA34_001685"
     CDS             12468..13130
                     /locus_tag="ACHA34_001685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8792304.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            13365..13508
                     /locus_tag="ACHA34_001686"
                     /pseudo
     CDS             13365..13508
                     /locus_tag="ACHA34_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            13658..13858
                     /locus_tag="ACHA34_001687"
     CDS             13658..13858
                     /locus_tag="ACHA34_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8792305.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(14112..15791)
                     /locus_tag="ACHA34_001688"
     CDS             complement(14112..15791)
                     /locus_tag="ACHA34_001688"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8792306.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(15793..16005)
                     /locus_tag="ACHA34_001689"
     CDS             complement(15793..16005)
                     /locus_tag="ACHA34_001689"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFM8792307.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            16398..18128
                     /gene="efrA"
                     /locus_tag="ACHA34_001690"
     CDS             16398..18128
                     /gene="efrA"
                     /locus_tag="ACHA34_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFM8792308.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            18125..19885
                     /gene="efrB"
                     /locus_tag="ACHA34_001691"
     CDS             18125..19885
                     /gene="efrB"
                     /locus_tag="ACHA34_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFM8792309.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            19980..20177
                     /locus_tag="ACHA34_001692"
     CDS             19980..20177
                     /locus_tag="ACHA34_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792310.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            20330..20815
                     /locus_tag="ACHA34_001693"
     CDS             20330..20815
                     /locus_tag="ACHA34_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFM8792311.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(20919..21512)
                     /gene="clpP"
                     /locus_tag="ACHA34_001694"
     CDS             complement(20919..21512)
                     /gene="clpP"
                     /locus_tag="ACHA34_001694"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFM8792312.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            21692..22219
                     /locus_tag="ACHA34_001695"
     CDS             21692..22219
                     /locus_tag="ACHA34_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792313.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            22310..23047
                     /locus_tag="ACHA34_001696"
     CDS             22310..23047
                     /locus_tag="ACHA34_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8792314.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            23051..23968
                     /gene="miaA"
                     /locus_tag="ACHA34_001697"
     CDS             23051..23968
                     /gene="miaA"
                     /locus_tag="ACHA34_001697"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFM8792315.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            23970..25199
                     /gene="hflX"
                     /locus_tag="ACHA34_001698"
     CDS             23970..25199
                     /gene="hflX"
                     /locus_tag="ACHA34_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFM8792316.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         8395 a         4108 c         5300 g         7412 t
ORIGIN      
        1 caagcttttg aacaccgttt tatttgtctc acaccctttt ccttcctact tgtaaccttg
       61 ctcccgtgtt agaaacatgt tacactaaat atgtgaaatt attaacaagg agcgtcgtta
      121 tgcgagaaaa aacagataca ctgccaaaag caactgcgaa aagattacct ttatatttac
      181 gctacttgaa gatgctggat gactcaggta taagtagaat caaatcaaat gagttcagcg
      241 aaatcaccca aattccttct gcaacgatcc gcagagactt ctcgcaatta ggcgagctag
      301 gcagaagcgg atatggctat gatgtccctt ttcttatcga tgtattcaat aatatcctta
      361 atactaaaga agaaaaacga atcgcattag taggatatgg aaatcttggc aaagcattga
      421 aacacaacaa ttttagaaga aatgaaaatc taaatatcgt gtgcgtcttt gataatgatc
      481 cagcactgat caatcgagta attgatggag aaatgatcta tccgattgat cgtttcgcgg
      541 agatagccaa agcaaaaaat gtgacagtag caatctctac ggtaccaagt aaatattcac
      601 aatctgctat tgatgaaatt gtcaaaggaa atgtaaccgc tatacttaat tttgcgcctg
      661 acagagtaac tgtcccagct tatgtcaatg ttcaatacat tgatttgaca accgagttgc
      721 agacgcttat ttactttgat gagaattact cagaagtttt ttcttaggaa aatttgagta
      781 ggcttattga tcttgcgaga actcgtcgta attctttcaa aaatttttta gtactaaatg
      841 aagtggtttt ttcacgacca cttgttgact aaaaaaaaaa accattgtag aatgaagcat
      901 aacgctgaac aagaggagta actggcacat tcattcttag agagagaact atctgctgaa
      961 aggttctcaa tgacaaccag tgaaggtagc ttgggagttt ctgtcttgaa tggagtagag
     1021 atagacgagt cgtgatcgtt aactcacttg agaggcaata atgattgatt attgcaattt
     1081 aggtggtacc gcgtaaatca tacgtcctag aatacgaaag tattttagga cgttttttag
     1141 tttaaaggag gaaaacagca tgacggaaga aaataaccta tcaacaaaat ataatccgca
     1201 agaagttgaa gctggacgtt atcagcattg gttagaacaa gatttgttta aaccgagcgg
     1261 agataaaaaa gcaaaacctt attcaatcgt aatcccgccc ccaaatgtta ctggaaaact
     1321 tcatttaggg catgcttggg atacgacctt gcaagatatg ctgattcgtc aaaagagaat
     1381 gcaaggatat gatactttat ggctgcctgg tatggaccat gcaggaatcg cgacacaagc
     1441 aaaagtagaa gcgaagctta gagagcaagg gatttctcgt tatgatctag gtcgtgaaaa
     1501 gttcatcgat caagtgtggg aatggaaaga agagtatgcg agccacatcc gcgaacagtg
     1561 ggctaaaatg ggcttatctt tagattacag tagggaacgt tttacattag atgatggatt
     1621 atctgaagca gtacgtaaag tttttgttac cttatacgaa aaaggtttga tttatcgtgg
     1681 tgaatatatc atcaactggg atcctcaagc acgaacagct ctatctgata tcgaagttat
     1741 tcataaagat atcgaaggcg ccttttatca tatgagctat gaactagcag atggatcagg
     1801 cgtagtagaa attgcgacaa cccgtccaga aacgatgttg ggagatacgg cgattgctgt
     1861 tcatccagaa gacgagcgtt atcaagcgct catcggcaaa aaagtgattc tacctttagt
     1921 caataaacaa atcccaatca ttgcagatga atatgtagat atggaattcg ggacaggtgt
     1981 agtaaaaatc actccagcac atgatccaaa cgactttgag gtaggtaatc gtcatgatct
     2041 tccacgcgta aatgttatgg atgatgatgg tacgatgaat gaactagcag gaaaatatgc
     2101 tggaatggaa cgttttgctg ctcgtaaagc aatcgtgcaa gatctgaaag aaatcggtcg
     2161 attgattaaa atcgatacga tgatccacag tgtgggtcat tccgagcgga caggtgtcgt
     2221 tgttgaacct cggctgtcta ctcaatggtt tgtaaaaatg gcgccgttag cgaaaaaagc
     2281 cattgataac caagaaacag atcaagcagt ggaattcttc cctccaagat tcaatcaaac
     2341 gtttttacgt tggatggaaa atgtccatga ttgggtaatc tcacgacaat tatggtgggg
     2401 acatcaaatt cctgcatggt atcacaaaga aacaggtgaa atgtatgtag ggatggaagc
     2461 accagcggat agtgaaaact gggtacaaga tcctgatgtt ttagacactt ggtttagttc
     2521 agctttgtgg cctttctcaa caatgggctg gccagatacg gaaagcgaag attataagcg
     2581 atacttccct actagcactt tagtaaccgg ttatgacatc attttcttct gggtaagcag
     2641 aatgattttc caaagcttgg aatttactgg cgaacgtcca tttgagaatg tcttgattca
     2701 tggattgatt cgtgacgaac aagggcggaa gatgagtaaa tcattaggta atggaatcga
     2761 tccgatggaa gtaatcgaaa agtatggagc agatgctctc cgctggttct tatcgaatgg
     2821 ttctgcacca ggacaagatg tccgcttcag ctatgaaaaa atggatgctt cttggaactt
     2881 tatcaacaaa atctggaatg cttcacgttt cgttatcatg aatatcgaag gaatgaccgt
     2941 agaagagatc gatttatctg gagaaaaatc agtggctgac cggtggatcc ttactcgttt
     3001 gaatgaaacg atcgaacgag taactgagct atttgatcgt tttgaatttg gtgaagcagg
     3061 tcgacaactg tataacttta tctgggatga tttttgtgac tggtacatcg aaatgagtaa
     3121 agaagtactt tatggagatg atgctgtttc aaaacaaacc acccgtagca tcttagtcca
     3181 tacacttgat cagatcctac gtttattgca tccaatcatg ccattcgtta cagaagaaat
     3241 atgggaacat attcctcatc agggaaattc tctagtagtg gcagaatatc ctgttgtacg
     3301 tccagagttt gatgatgaaa cagcttctaa agggatggaa gtattgaaag aattgattcg
     3361 agccgttcga aatattcgtt cagaagtgaa tacaccattg tctaaaccaa ttacgttgct
     3421 aattaaaaca aacgatgcta aaatcgatca atttttagtt gaaaatacaa gctatatcga
     3481 acgcttctgt aatcccgaag agttgacgat atcaagtgag atcactgcgc cagatctagc
     3541 gatgtcagct gttctgacag gtgcagaaat cttcttgccg ttagcaggat tgatcaatat
     3601 tgaagaagaa atcaaacgat tagaaaaaga actggaaaag tggacaagcg aagtaaaacg
     3661 tgtccaagga aaactttcta atgaacgttt tgtttctaac gcgccggaag aagtagtaga
     3721 ggcagagcgt gcaaaagaaa aagattattt agagaagcaa acggctgtca aagaacgaat
     3781 cgaaagtttg cgtactattc aataagaata gaaaaagaga gtgtgaaatc agctagctat
     3841 tactaaaagc tagctgattg aacatcgtta caggaaagag tttgagcctc actcttagct
     3901 tcgattcata aggagatgtg acataaattt tgtcgcatct cttttttagt ctactcttat
     3961 tatgtaggag aaggagggag gtggatcatg tatcaagagt caataaagta gttgataaaa
     4021 tcgttcctct ctctgtttct tttctctttg tcatactttt atttttctta tgtgttctat
     4081 ttgttataat aaaagagaaa tgggggaatt gttgatgacg attgaagaag caatagaatg
     4141 gatccatagt cgattaccat ttggttcacg tcctggcctt gaccgagtcg aagctttatt
     4201 agaagtggta gggcatccag agagaaaagt aaaaacgatc catatcgctg gaacaaacgg
     4261 aaaaggttct actgtagctt atttgagaag cctattagaa gaggctgggc tgacagtagg
     4321 tacatttact tcaccttata tcgaaacgtt caatgaacgg atcgcaatca atggcaaaag
     4381 cattccagac aatgatttga tccagcttgt aaaaaaatat cagccactga tcaaacaact
     4441 tgatcaattt gaagcagtag ctggtattac tgaattcgag acattaacag caatggcatt
     4501 tgattatttt ttgatgaaga aagtggacgt agcgattatc gaagttggct taggcggctt
     4561 attggattct accaatgtag ctgtaccaat gctgacggga ataacaacga ttggacttga
     4621 tcataccgaa atacttggag atacaattga agaaatcgct gcacaaaaag ctggaatcat
     4681 caaacaaggc gttccagtag taacaggaaa tattgcccca gaagctttta ctgtcatcca
     4741 acaaacagct aaagcaaaaa atgcaccgct ttatgtatac ggggaaaatt ataaaataaa
     4801 ctatctgcat cctgatgaga tgtggggaga agtatttgaa ttttttaatg aaataggaaa
     4861 gatccctcaa ttaaccactc cgcttttagg gcaacatcaa gctgaaaatg caggagtagc
     4921 catcgaattg tattttgttt actgtcaatt ggaaaagctg cctttccgag aaaaagatgt
     4981 tttgaaggga ttgaagaaat ctcgatggcc aggaagaatg gagcgtttga gtaacgagcc
     5041 attagttgtt ttagatggtg cccataatac gcatgcaatg aaccggcttg ttgagaattt
     5101 gaaaaaagaa ttcaaaggat atcaaatcaa cattttgttt tccgccttac gaactaaaaa
     5161 tgttagagaa atgctagaac gattgcagca agtaccaaat gtccatattt atttgacgac
     5221 ctttgaacat ccaaaagcca tcgaattaag tgaagtatca gatttagcaa gtgagagagt
     5281 atcaatcgtt tcattgtggc aattcggatt agcagatata ttggaaaaga tgacaaatga
     5341 agacgtatta gttgtgaccg gttcacttta tttcatttca gaagttcgcc atttattaat
     5401 acaattagga ggagatcatg aagtataatg cagtgatttt tgacatggat ggtttgctgt
     5461 tcgacacaga gttagtgtat tacgaagcca cacaagtagt agcagacaag atgggttttc
     5521 cttatgataa agacctgtat ttaaaattct taggtgtttc agatgaagaa gtatgggcga
     5581 attatcatat gattttctcg gaatttggca aaacaagtgt acaaagattc atagatgatt
     5641 cataccagga aacgcttgaa cgtttttcgc aaggagcggt acaaatgaag cccggtgtgt
     5701 tggaactcct ctctttttta gaagaacatc agattcccaa agttgtcgct tccagtaacc
     5761 aacgagcagt cattgaactt ttattgaaga agaacaatct gtttgaacga tttgagacga
     5821 ttgtttctgc agaaaatgtt caacgagcaa aaccagaccc agaaattttc ttgttagcta
     5881 atcagaagct aggtacagcg aagaaagaaa cattgatttt agaagattca caaaatggta
     5941 ttttagcagc ttctgcggct gagatcccgg tagtcatggt accagatctg cttccaccat
     6001 cccaagaatt gatcaaaaaa acagctgggg ttgtttcttc tttacatgaa atccctgcat
     6061 tgttaaaata aaaatccatc tcttttcgta tcttatttta gataagatag aaaggagatg
     6121 tttttttggg aaaacgattg atcaaagaag tacctactag ctctttacca agggaacgta
     6181 tggaaattta tggcgcggaa gctttatcgg atcaggagct tttggcaatc ttactgcgta
     6241 ccggtcaaca cccttatagt gtgatgtcga ttgctggaaa tctgttaaaa acgtttggag
     6301 gactggcctc tttacgacag gctactttgc atgagttaga agagattcga gggataggac
     6361 gcgtgaaagc aatcgaaatc aaagccttga tcgaattagg tcgacgaatt cagacggatc
     6421 atgaaagagc ggctccaccg gttcgtacca gctacgaatt ggcccagcaa ttgattctgg
     6481 aaatgaaaga tcacaaacaa gaacatttgg tttgtattta tttggacacg aagaatcaag
     6541 tgatcttaaa aaaaacagtg ttcatcggtt cgctgaatca gtcagtcgct catcctagag
     6601 aaattttcca ttatgctgta cgttattgtg cagcaaggat cgttttagcg cataatcatc
     6661 cttctggtaa tgttttaacc atagtataat aaataatagt agttaaagta gtttaagagg
     6721 agatattaaa gggatatttt actttaaata aaaattaaca aggtttataa tttgtagtag
     6781 ttaaagtatt taaagaggtg ggaacatgtg aaggttcaaa ggatagaagt ggagaataag
     6841 ccgtatccat tgtatttatt actagataaa gaataccagc taatagaacc agtaatgaaa
     6901 tttattaaat acttagataa tactggtaag tctcctaata ccattaaagc atactgctat
     6961 catttaaagt tgctgtacga gttcatggaa cagagaggtg ttattcttaa tgatattaac
     7021 tttgagttgt tagcagactt cgtaggttgg ttgagatatc cttcagcatc aaatgtaatt
     7081 gatcttcagt caaaaaaagc cataagagaa gaaacgacag tgaatacaat tttaaatgta
     7141 gttatgagtt ttcttgatta tttaagtaga ttaggagaat ttaaatcaat tgatgtattt
     7201 aaacaagcaa agggaagaaa tttcaaagga tttttacatc atgttaataa gggtagatac
     7261 caaaagaatg tcttaaagtt aagggttaaa aagaaacaga taagaacatt gagatcagag
     7321 gaagttaagc aaattattga tgcttgtcat acgaaaagag ataaattaat tttaatgctt
     7381 atgtatgagg gtggtttaag aatcggtgaa gtgttatcgc ttaggcttga agatattgtc
     7441 acttgggaca atcaaatcca tttaacacct agagatgtta atgttaatga agcttatatt
     7501 aaattaagga aggaaagaac aatacatgtg agtaaagaac ttatgtcact ttatacagat
     7561 tacttgatat atgagtatag tgaggaattg gagcatgatt atgtttttat ttccttaaaa
     7621 gaaggctatt ttgggaaacc actaaagtac caaagtgttc ttgatctagt tagaagaata
     7681 gttaaaagga ctggaataga atttacatca catatgcttc gccacactca cgcaacgcag
     7741 ctaattaggg aaggatggga tgttgcgttc gttcaaaaga gattaggtca cgcacatgtt
     7801 cagacaacgt taaataccta tgttcatctt tcagatcagg atatgaaaaa tgagtttaat
     7861 aaatacctcg agagaaagga gcataagaaa tgaatgcttc tagtaaaagg aaaattatta
     7921 gtcagagtga gattagcaaa aaaatagctg taatgaatga agaaatgcag gggttttggg
     7981 ctaataatag ttgggatata agaaaatgtc cacatccttc tgccatagaa ttaagtaaga
     8041 atcctgcttt aaggaatcgt tgggttcgtt ttgaacgtgt taaaaatctg tggttaagaa
     8101 cagaattgaa atatttttat ttttaccatt taaacaatgg aatatggaat gcaaaaactg
     8161 tctggattag aaaaggaaca gtaattaata aaatgttaga tttcttagat ttaaagtatc
     8221 ctagcattac ttcaattact gaagttccta ttgaaaaagc aatgacggag tatagaactt
     8281 atttgacaaa acggggtgtt agaattacca ctactaatta taagattact gctaatcaag
     8341 aaaaaacacc tgtaaaagct aattcctact atgttactaa tctaaaacaa tttatggagt
     8401 tttatgagaa cttttatttt gatggagagg agtgggataa agacgtttgg gatagacgta
     8461 acttaccttt gccagatgat aaggttaacc caacacaata tgaatataca attaacttta
     8521 aagggtttcg gaatacatat tttaaacaac ttgtaaaaag atattgtaag ttgagattga
     8581 acatggatag cttttcctat gtaagtgata ttgcccaaag acttaaagag ttctttaatt
     8641 ttctggacat gaaatttaaa caagttcaga gagtacacca attaacgaga gtggaaattg
     8701 aagcatattt aagtgaacta aacatgatgg gaataaaacc tagtacaata actgggagga
     8761 tctctatatt ggaaggacta tttagtaccc ttcttaggct agaatgggat gatgttcctt
     8821 ccaaaatatt aatttattct gaggactatc cgaaaatacc aagagcaaaa ccacgcttta
     8881 tagatgaatt cgtcctagag caattgaaca gtcatcttga taaattaccc gaatatatag
     8941 ctacgatgac tatgattgtt caagaatgtg gaatgaggat aagtgaattg tgcaccttga
     9001 aaaaaggctg tctattagag gacaaagatg gagatttctt tttaaagtat tatcaatgga
     9061 aaatgaaaaa ggagcatata gttccaatat ctaaagaggt agctttactt attaaagttc
     9121 gggaagataa agtttcagag gaatttccag atagtgaata cctctttcca agaaaagatg
     9181 gatcgccatt aaaacaagaa acatttagag gtgagttaaa taaattagct tatgagcaaa
     9241 atatagtgga taaatcaggt gagatttata gattccatgc ccatgccttt cgccatacag
     9301 taggaacaag aatgattaac aacgggatgc cccagcatat tgtgcagaaa tttttggggc
     9361 atgaaagccc agaaatgaca agcagatacg ctcatatctt tgatgaaact ctaaaaaatg
     9421 aatttactaa atttcaggaa aaactggtta ccaataatgg agatgtgctt gatctagatg
     9481 aagataatga agtcgatgat gtagagcttc aatggttcaa gaaaaatata aatgcacaag
     9541 tgcttccaaa tggttattgt agattgccag tagtagcagg tggttgtcca catgcgaatg
     9601 catgcttaga ttgcactcac ttctgtacca gtaagcaatt cttaccacag cacgaagaac
     9661 agttagagcg tacagaagag ttattagcca tagctaagga taaacaatgg caaagacaag
     9721 tagagactaa tagccgtgtt aaagagcgtt tagaacaaat cattggaagt ttgacggggt
     9781 aattatcaat ggataaacaa gttagaaata caacagaaat tgtacgtttg gcgaagcaga
     9841 aatcaaaaaa gacaagggaa aaagtagaca aagcgatttc taaattttcg attgaaggta
     9901 aagttattaa ttttaattca atagcaaagg aagctaatgt ttctaaatca tggctttata
     9961 aggaacacga tattaggcaa agaatcgaat cccttcgtga gcgtcaaata acagcaaatg
    10021 tagtctcaaa acccaagaaa agttctcgtt cggaggaaat ccttattaaa accttaaaaa
    10081 gaagagtaat ggaattagaa aaagaaaata aaaaattaca gaaccaaatt caaaaattat
    10141 atggagatct gtataataaa gaataattat taatctgtag acaaattgtg aaaggatgta
    10201 cttaaacgct aacggtcagc tttattgaac agtcatttag gtatatgtcc aatctagggt
    10261 aagtaaattg agtatcaata taaactttat atgaacataa tcagttacga ggtgaaatca
    10321 tgagcaattt gattaacgga aaaataccaa atcaagcgat tcaaacatta aaaatcgtaa
    10381 aagatttatt tggaagttca atagttggag tatatctatt tggttcagca gtaaatggtg
    10441 gtttacgcat taacagcgat gtagatgttc tagtcgtcgt gaatcatagt ttacctcaat
    10501 taactcgaaa aaaactaaca gaaagactaa tgactatatc aggaaagatt ggaaatacgg
    10561 attctgttag accacttgaa gttacggtta taaataggag tgaagttgtc ccttggcaat
    10621 atcctccaaa aagagaattt atatacggtg agtggctcag gggtgaattt gagaatggac
    10681 aaattcagga accaagctat gatcctgatt tggctattgt tttagcacaa gcaagaaaga
    10741 atagtatttc tctatttggt cctgattctt caagtatact tgtctccgta cctttgacag
    10801 atattcgaag agcaattaag gattctttgc cagaactaat tgaggggata aaaggtgatg
    10861 agcgtaatgt aattttaacc ctagctcgaa tgtggcaaac agtgactact ggtgaaatta
    10921 cctcgaaaga tgtcgctgca gaatgggcta tacctctttt acctaaagag catgtaactt
    10981 tactggatat agctagaaaa ggctatcggg gagagtgtga tgataagtgg gaaggactat
    11041 attcaaaggt gaaagcactc gttaagtata tgaaaaattc tatagaaact tctctcaatt
    11101 aggctaattt tattgcaata acaggtgctt acttttaaaa ctactgattt attgataaat
    11161 attgaacaat ttttgggaag aataaagcgt cctcttgtga aattagagaa cgctttatta
    11221 ctttaattta gtgaaacaat ttgtaactat tgaaaataga aagaaattgt tccttcgata
    11281 gtttattaat attagtgaca tttgcatgct tcaaagcctg tcggaattgg tttttagtga
    11341 aaagaacacg atattcacgg tttacccact tataaacaaa agatcgatac tttttgtagt
    11401 ccttctttga aatcaatggt tgatgtcgtt caagaacaat caatacagag tctacacttg
    11461 gcttaggatg aaaatatagt ggtggtactt ttttgagcat ttttatatcc atctccacca
    11521 ttaatagtaa acccaaagct cgttgcagat tttgcaatct tttcgcaaat cccttctcaa
    11581 cgataagata gctatattta gcctgacttt caaaggtaat tcttttgaca atatccgtac
    11641 tgatgttata aggaatatta ccatatatct tatagtttat atgttttggg aaggaaaatt
    11701 ttagaatatc cgtttgaatc acttttatat tctcagaggg gtttaccgct tctttagtca
    11761 cttgacataa gcctccatca atttctatag cagtaactga tcgactcatt ttgactagct
    11821 ctttggtaaa atgtcctttt cctgatccga tttctattac gttgtcttgt ttactgatat
    11881 tcgtgtgatt caatatttct tttacatgct ttttagaagt aataaaattt tgcgtgtctt
    11941 tagggttttt ctggttcatt ataaccttct ccttactggt tataatgaac tgacattaag
    12001 cgttcattat aaccaattaa ttttgatttg gttgataacg aactttatta ataacaaata
    12061 tagaaaaagt acccatactt ttatctcctt tccatttttt tcattatgaa tgagataaag
    12121 taatttctac tactgcgata ctggtgcaca taattgaaac ctccttatgt aactgaatat
    12181 gattgtatca tcttttcatt ttagaataaa cctttatatg taattttgca cttgacactt
    12241 cagatggacg ttttcttcaa aataatatgt tttgaaactt gataagagaa gccactgtct
    12301 ttactaaaaa gtaaaggctg cgataaagga cagtgacttt aataaagttg cactgttgta
    12361 ttgtctttgg ataaaattgt ctaaagggaa gttttatccc caacgaaaaa acatctaaag
    12421 tggtaagtaa tgaaagaatg tcgataaagt aggaatgggg gatctttttg aatacaatag
    12481 aatcacagtt tgataaggtt gcagaagatt acgatttcgt gaatgagctt ttgaatgatt
    12541 attcattctt tgtgtctaat atgtctccaa agaaaggcag agcattagat atcggatgtg
    12601 gctcgggttt gttagtggag aaattagcaa gttattatga tgaagtggta gggattgata
    12661 tttctaatca aatgctcgat cttgccaaat ctaaacgtca actaacaaat acggtctatc
    12721 tgaatatgaa tgcagaacaa cttaatttta atgagaagtt tgattttatt gtaagccgaa
    12781 caacctttca tcatttggat gatatagcca gcgtaataca gcaaatgaag gaactgttga
    12841 atgaggaagg aagaatagtt attcttgata atgtatctga agtcgagaca ccgcctacct
    12901 atgtttataa attaggggca atccaagagt ttttaccgca ttgtttcaaa tttggaataa
    12961 aaaatgcgat tagaatttat aatcacaaca catctaaatc atggcttgag catttagctt
    13021 cagataagta cctatctgaa caaaattatt acgatttata tgaaaagttg ttgcctggat
    13081 gccaattcca taaaatgggt tgggcaatgg gggttgtctg gacaaaataa cttaaagctg
    13141 tggctttgaa aagttgaatt tgtgattgtt ttcagagcta tcgggtgctt tagtataaat
    13201 aacaatgaat aaaaaatcag cctgtatctt atgttactat taagtaattt aagatacagg
    13261 ttttactatt tttgaaataa gtaccttcca cttcaaatac atgtattcaa agtgaaagta
    13321 ttgatttaaa tgattctttt gtaatttact cttgataata tgaaattcca tcgcagcagg
    13381 atatgaattt taccaaaagg atacaaaagt gtggagaaat gatggggatc actgtactgg
    13441 accacttgat catcggacgc aaacgatatt ttagtttgag agaagaagga atgatggaag
    13501 agaagtaagt tgctttcttg caaaaaaaat gaagcaagtg tacaattata ttgtaatttt
    13561 gtttgggaat agaagttgaa acatctgttg cacctctatc gtacacgagc aatgttacaa
    13621 tttaaattag tgcttttggc acgaggagga tattattatg gaaaacggaa cagtaaaatg
    13681 gttcaacgct gaaaaaggat ttggatttat ctcacgtgaa gatggaagcg acgtattcgt
    13741 tcacttctca gctatccaag gtgacggatt caaaacatta gaagaaggtc aagcagtgac
    13801 tttcgacgtt gaagaatcag accgcggccc acaagctgca aacgttgtaa aagcttaatt
    13861 ttatctgtaa atcaaattga tggtagacat caatttttac gagagaacaa agcgagtaac
    13921 aaaaatacct gccacaagag acaatttctc ttgtggcagg tatttttttg tatgaaaaag
    13981 taaataaatt taagaggcta tgaaagagcc accttgtatc aaggaataag aacaaacaaa
    14041 aatggcttct catgtttttc atttgttttc tcttatattc aggacaaggt gctgatttca
    14101 taacctctta tttattcttc atctggtgtc attaccattg gcaaaatcat tggatgtctt
    14161 tctgtacgtt cgaataagaa aggctgaagc gcgccagtca tcgcttcttt caatttatat
    14221 tctgaacagt caggtccatt caaacattct cttaaagcgt ggaataagat tcgctgagct
    14281 tcattgatca tttctcctga ttctcgcatg tagacaaatc cacgagagag aatatctgga
    14341 ccagccaaga tttctttttt cttgatatcc actgtcgcga cagctaatac aagaccttct
    14401 tctgaaagca ggtgacggtc acgtaaaacg acattaccaa tatcaccgat cccgctacca
    14461 tcgatataca catctccagc cacaaaatgc ccagcaggac gcgcagaatc gctagttaac
    14521 gctaaaacat cgccattttc catgatgaag cagttttctg caggaacacc tacatcttgt
    14581 gctagagaag catgtacttt caacatgcgg aattctccgt ggacaggaat aaaatatttt
    14641 ggcttcatca aacgaagcat taatttttgt tcttcttgtc caccgtgacc ggaagtatgg
    14701 atgttattga tttttccgtg aatcacttct gcaccagctt cagacagcaa attgatcaaa
    14761 tgattgacac ttgttgtatt accaggaata ggtgaacttg aaaagatcac tgtatcgtct
    14821 ggctggattt ggatttgacg gtgagtacca ttcgcgatgc ggctcaacgc ggccatcgct
    14881 tctccttgag aacctgtaca taggatcatc gtttcattgg caggcaagcg attgatttcg
    14941 tgtgcatcta caaatgtgcc gtctggaact ttgatatatc ctaaacgttg accgttgacg
    15001 atggcatttt ccatgcttcg tccgaaaaca gcgattttac gaccagttgc cacagcggca
    15061 tctgctgctt gctgcaaacg gaagatgtta gaagcaaagc tggcaaaaat gatacgtcct
    15121 tcgatttttg aaaagatctt caaaatagaa tgcccaatcg ttttttctga tttagtaaat
    15181 gttggaatct cagcatttgt actatcagat aaaaggcaca agactccttc ttccccgata
    15241 cgagccattt tatgcaagtt tgctggttcg ccaacaggag tgaaatcgaa tttgaagtct
    15301 ccagtcgcta cgatatttcc agaaggtgtt ttcaccacga tccctaatgc atctgggata
    15361 ctatgggtcg ttctgaaaaa gctgacagaa gtctttctaa aacggatgat cgtatcttca
    15421 ttgatctcat ggagctctgc atctcgaagc aagccatgct catctaattt gtttgtgatc
    15481 aacgctaatg cgagaggacc agcatagata ggaatatttg cttgtttcag caagtagggt
    15541 attccgccga tgtggtcttc gtgcccgtga gtaatcacca aggcttttac tttgtgtaag
    15601 ttttgtatga tgtagctgta gtcgggaatg acatagtcaa ttccaagtaa atcgtcttcc
    15661 gggaatttga tcccggcatc aatcaaaatg atttcatctt ggaattgtac cccatacgta
    15721 tttttcccga tttcacctaa tccaccaata gcaaagacgc ctgtctcgtt atttttgatg
    15781 gaaagtttca tattaaaact ccactaatga gaaatcagca ttttcttttt cgtactctag
    15841 atgacttcct tctagaagtt gaacgaattc aatattgtat ggggtatttt cttccacacg
    15901 ttggcgagcg tctacatcgc tttttgcctc aatgtaaaga gattttgttt cttcccgttt
    15961 agggttgcgt acttttgttt cttgataata aactttatag atcattgttt tgtctccttt
    16021 atctgcgtaa agcagtgatt tagctagttg atttttttag acaacacaaa acgaggggac
    16081 aagccacctg gtttttcata tgctttgact agaatactgg attcgagtca aagagacaag
    16141 cctcgttttt gcttatctgt tcggatattt tttcaaccgt accttctgta taaaaaatat
    16201 acaacaatta attgttattc tagcataaaa cagcaaataa gtatagaaat caactagatt
    16261 gaaagaaaaa atcaatgttt atcatcccgc tataatcata atgacttttt tatgagagaa
    16321 gtgttctatt aataggctcg ttgtgctatc atttttagta atgaaaatgt aacaaaagat
    16381 taaaaggaga tgagaaagtg aaactaatgt ggcattatac catgcgttat aagaaattct
    16441 tgtttttcaa tttcatatgc gtatttggtt ttattttgat tgaattagga cttcctacga
    16501 ttttagcacg tatgatcgat gtaggaatcc gcaatgatga ttttgattat gtgaagcaac
    16561 aagggatatt tatgatcgtt attactgtaa tcggtattat tttgaatatc ttgttaggtt
    16621 attttggttc gaggattacg actaatatcg tagcagatat tcgagatgat ttgttcgaaa
    16681 agatccaaag ttattcgcat caggaatacg aaaatctagg cgtatcttca ttgatcactc
    16741 gtacaacaaa cgatgcttat cagatcatgt tgttcctgca aaatatttta cgtatcggtt
    16801 ttatggcacc aatgatgttc attgtcagtt tgtacatggt catgcgtaca agtgtgacac
    16861 tcagtttgta tgtagtaggt gcattacctt tactattatt ggcagtcgta gcgattgcta
    16921 aggtgtctga accgttatct aaaaagcaac aaaaaaactt agataaaatc aacagtatct
    16981 tgcgggaaaa tctttcagga ttgcgtgtga ttcgtgcatt cgtcaatgaa aagtttgaag
    17041 aaaaacgttt tgctgtagtc aatgagaatt atgcttctag ttcaaaaagc ttattccgtt
    17101 tgatggctgt tgcacaaccg ggtttttatt tcttatttaa tatcgtaatg gtattgatca
    17161 tctggagcgg ggcagtgcaa atcgatgcag gcgagcttcg tgtaggagat ttgattgcgt
    17221 ttatcgagta tatattccat gccttgttct cattcatgtt atttgctagt gtgttcatga
    17281 tgtatccaag agcagctgtt tcagctggac gtatccaaga agccctagat gccaaacctt
    17341 cgattgtaga agatccaaat ggtatatctg atacaaaaac taaagggtat atcgaatttc
    17401 aaaatgtaac atttgcttat ccaggccatt cgcaagagcc agtgatacga aatgtcagtt
    17461 ttacagcctc tccaggagag accgttgctt ttattggaag taccggtagc ggaaaatcta
    17521 cgttgatcca attgatccct cgcttttatg atgtgagtga aggaaagatc ttgatcgatg
    17581 gagtagacgt gagagattat cggctaagca agttgcgaga taagattggg tttattccgc
    17641 aaaaagcgct gctatttact ggaacgattg cagataattt gcgatacggg aaagaagatg
    17701 ctacgcaaga agaaatggaa cgtgcagctg agattgctca agcttcagaa ttcatttctc
    17761 gaaaaccaga tggatttaat gaacatttat cagaaggtgg ttccaatttt tcaggtggac
    17821 aaaaacaacg attggcaata gccagagcca tcatacgccg tccggaaatc tatatttttg
    17881 atgacagctt ttctgcatta gattatcaaa cggatgcgaa gttacgtgct cgtctgaaaa
    17941 aagaaacgac agaatctact gtattgatcg tagcacaacg tgtgggaacg atcatgcatg
    18001 cagataagat catcgtatta aatgaaggag aagtcgtcgg tatggggacg catcgggagt
    18061 tgttggaaaa atgcccgatt tattatgata ttgccgcttc tcaattatca aaggaggaat
    18121 tagcatgaaa aaaacctttt cttccttaaa acgtttagct cgatatatca gaccgtatcg
    18181 tgagactttt cttttagtga tcctatttac agttctgaca gtggctttca acgtggcttt
    18241 gccatatgtt acaggacttc ctactacaga aatcagccgt aatattgcca atggcgaatc
    18301 aatcaatttt tcttatatat tcaaatgtct catttggatc acagttgttg gaatagggta
    18361 ttgtatttcg caattacttt ctggtgtttt gatgacaaat gtcgttcaaa gtgccatgca
    18421 tgatttgaga agagacatag aagaaaaaat caatcgtctt ccggtttcat atttcgataa
    18481 aaaccagcaa ggaaatattt tgtctcgtgt gacgaatgat gtagatgcag tcagcggtgc
    18541 tttacaacaa gcttttattg gtatcgtaaa tgcgatattg ggaattacgt tggctgcagc
    18601 aatgatgttt tatatccagc cgatgatggc gctgatctct atgatcatga ttccaacatc
    18661 gatctggatc tcaaaacgag tgatcaatgc ttcgcagaaa tattttcaaa gcatgcagaa
    18721 ttctcttggt gagctaaacg gctacgtcca agaaaatatg acaggtttta gtgtattgaa
    18781 agtatatgga cgcgaaaaag aaacattgga aggattcaaa aaagttaatc acagcttgaa
    18841 atactttggg tttcgtgcag cattcatttc tggcttgatg atgcctctag ttcaattaac
    18901 tgcctatgct acttatattg gtatggctgt tctaggaagc ttttatgtta ttactggggc
    18961 gatcgttgtc ggtcaactac aagcattcat ccaatatatt tggcaaatca gtcagccaat
    19021 ggggaacgtg acacaattgt cttcagtttt gcaaagtgca tcagcggcaa cgaaacgtgt
    19081 attcgagatt ttagatgagc cagaagaaaa agaaaacaaa gtagatgctt cactgccaga
    19141 aacgatttta ggagatgtga cattcgatca tgtcgatttt gcatatgatc cgaagaaacc
    19201 attaatccaa gatttgaatt ttgaagtgaa agctggtcaa accgtagctg tggtcggtcc
    19261 gaccggtgcc ggcaagacga cattgatcaa tctattgatg cgtttttatg acgtagacaa
    19321 aggagcaata aggattgatg gaatcgacac caaatcaatg agtcgcagtg atgtgcgttc
    19381 gctatttggt atggtcttgc aagatgcatg gctttatcaa ggaacgattg cagataatat
    19441 ccgcttcgga aagcttgatg cgacagatta tgaagtagtt gatgctgcga aaacagcaaa
    19501 cgtagatcat tttatccgaa cgatgccaga tggatatgat atggccatca attctgaggg
    19561 agacaatgtg tctttaggac aaaaacagtt gctgacgatt gcccgagcag tggtctccga
    19621 tcctaaaatc ttgattttag atgaagcaac aagttcagta gacacccgtt tagaagcgtt
    19681 gattcaaaaa gcaatggatc gcgtgatgga gggacggaca agtttcgtca tcgctcaccg
    19741 tttatcgaca atccgtgatg cagatctgat tctagtgatg gatcaaggtc agatcattga
    19801 aaaaggaacg catgagagtt tattggcaca aggtggattt tatgaaaaac tgtataacag
    19861 ccaatttgct gaggaagcag aataagtcat ataagaataa aacagtttaa aacttatttt
    19921 ttgttctcta ttcttgataa tggtaaagta aagttatcaa gaaaagtgag gacacggaga
    19981 tggaacagtt tttaaatgaa aaaattactg aagccaaaag agaagttgat cgtttaacgc
    20041 acgtctgtca gacgaagcct tttgattcag ctgatttttt tgaaaatgag tttcaaatcg
    20101 aacgcttggc agcacaaata gaagtgtacg aattaatttt aaaaaaagta atagaagaaa
    20161 atatagatgt acaataaaaa caggtaaaac gtcagttacc agatactgta aatagacatc
    20221 tggcctgagc aggttttacc tgtttttttt cttatgaaat aaatatatgc tatgatacct
    20281 ttattcaata ggattagttt ctacaaaaaa tgaaaggaga aaaataaata tggaacggtt
    20341 tatagcatca aatgccacag tgattggtga tgtgacattg tcagaagatg tgacgatctg
    20401 gtatcaagct gttttacgcg gggacagtaa ttggataaaa ataggacaaa gaacgaatat
    20461 ccaagatggt acaatcatcc atgtcgatca tgatgcacct gtagatattg cggagaatgt
    20521 gacggtagga catcagtgca tgctacatgg ctgtacaatc gaaaaagggg cgctgatcgg
    20581 gatggggacc acgatattga atcatgcggt tattggtgag aatagtttga ttggtgctgg
    20641 atcattagta acagaaggaa aagttattcc tcctaatgtc ttggcttttg gtcgcccagc
    20701 taaagtgatc cgtcctttga caaaagaaga aatacaaaaa aacaaagaaa acatccagca
    20761 ttatgtcgag ataggtcgga tgcatgcgca aggaaaattc aaggagcaaa gataggcagt
    20821 agcatccata aaataaaaaa ggatggtcag tttgagttga ccatcctttt ttatttggtt
    20881 tagataagag agcttaaaaa agcttccttt gaaacagatt aatttaatga agaactgttt
    20941 tccatgactt catcgatcaa tccatatgct tttgcttgtt cagctgtcat atagttgtca
    21001 cgatctgtat ctttttcaat cacttcaagc ggttgtccag tgcgttcagc taaaattttg
    21061 tttaagcgtt cgcgtgtttg caaaatatgg cgtgccgcaa tttcgatttc agttgcttgc
    21121 ccttgagcgc cgccaagagg ttgatggatc atgatctcag catttggtaa agcaaatcgt
    21181 ttaccttttg taccagctgt cagcaagaag cttcccattg aagcagccat acccataaca
    21241 attgtttgga catctgcttt gacaaagttc attgtgtcgt aaatagccat acctgctgta
    21301 actgaaccac caggagaatt gatataaaga tagatatctt tttcagaatc ttgagcgtct
    21361 aagaaaagca attgggcgat gatcgaatta gcaagatcat ctgttacttg gccgcttaac
    21421 atgatgatac ggtctttcaa taaacgtgag tagatatcgt aagctctttc acctcgtgaa
    21481 gattgttcaa tgactgtagg aattaaattc ataatagagt tcctccttga tttcgtttag
    21541 aaatagtgta ccataattta gtttctaata cctaaaatgt agtatacatt tttggtcaaa
    21601 aaaggtcaaa taaaaaactt aggcaaatta gtcgcataaa cccgctttat ggtatgataa
    21661 gcagagaaac aaaacaagga ggaacaacag attgatagtt ggtattactg gccttatcgg
    21721 agtgctgaca ggtgctgtag ttgtctatat cggcatgata ctgcaaatgc gtcacttgga
    21781 aaataaagaa cagcgtagac gtgaattgga attgaaagtc aaagaaatcg agacgctgaa
    21841 tcaattaaat aaaaagatca acgagatctt gcaaaaacgg acaacgctga tggaagaata
    21901 tgtcagcttt gatgcatttg atgattgtta tatcacgatt gacgattttg cgtatctcca
    21961 atcattttca gcgcaaaata gtttttatct gccaaattat attttagacg agctatttaa
    22021 gaatatctcc catcgaaaag tgattttatc tccagatgaa acaatgaaga ttggtggata
    22081 tgcttataaa ggcggacgcg tgattttaga gaattttacg gatagtctga ctgaaatgat
    22141 caatgaaaaa aaagctcagt tgaagactat gacgcgccag ccgattggtt tcttctcgaa
    22201 agatgatgtt tggagctgat attttgaagc attgccgttg cacatgagag cgtggtaatg
    22261 ctttcttttt ttcttaatga tacaatagaa ctgaacggag tgaagaaaga tgttacaaat
    22321 ctatgcccat cgcggaagca gcggcaccca cccagaaaat acgcttcctg catttgcaga
    22381 agctgtacga gtaggtgcag atggtatcga actagatgtc catctaagca aagatggtta
    22441 tttgatcgtt atgcacgacg aagaagtgga ccggacgact aacggtaaag ggctgattcg
    22501 agaaaaaaca ttggaagaac tcaaaaaatt gaatgcaggg ggcaaattca gcaaagaatt
    22561 tccagcagct aaagttcctg aattgagaga agtgttagga ctgctcctcc aaaaaaatta
    22621 ccgagggatg ctaatgattg aaattaaaac tgatcagtat gaatatcccg gtatcgaaga
    22681 aaaattagcc gaaacattaa aaagtagaga ttggcctttt caacatgcat attgcagctt
    22741 tcatttggat tctttggaaa aaatcgcatt actggaaccg gagatacagc tggacttatt
    22801 gatgagtact tctgataaaa agatccaact agcagcagaa accccttata tagaaggagt
    22861 acatccaaaa aatacttggt tgttcgaaca tccagtccag gcagctgatt ttccaaaagc
    22921 aattcgtgca tggacgatta acgatgaaga agaaatggcc aaatgtcaag aactcaaaat
    22981 agatggaata ataactgatt ttccggagaa agctcttcgg tggaaacaat caacgaaaag
    23041 taggtaaaat atggaaaaag tattagtgat cgtcggtccg acagcagtcg gtaaaacagc
    23101 gctaagtgtg gaactcgcac aactatttgg aggagagatc atcagtggtg attcattgca
    23161 aatctataaa aatctggata tcggtacagc aaaaatcaaa gaagaagaga tgaataatgt
    23221 tccgcatcat ttgattgatg tgatcgaacc tactcaacgt tactcagccg ctgattttca
    23281 gcattcaggt cgacaattga ttaccgagat tgcgaaccga gggcacctac ctatcatagc
    23341 tggtggtacg ggtctctata tccaatccct tctatatgat tatcagttag gagcgacaga
    23401 agaagcgaat cctgagatta gaaaaaagta tgaaacgtta gctgaaacaa ttggttccca
    23461 agcattatgg aatcagttga aagaaaaaga cccggcagct gctgagaaaa tccactggaa
    23521 caatcaacgc aaagtgataa gagctctgga agttttagag acgacaggac atagtatcac
    23581 ggcacctaaa gaagagccaa agcaattgta tgattacctc atgattggat tagatacgaa
    23641 gcgtagtatt ctttatgaac gaatcaatca aagagtcgat ttgatgctag cagaaggttt
    23701 agtagcagaa gcgcgtaagg tgtatgagct tggagaagtc caggccagtc aaggtattgg
    23761 atataaagaa tttttccctt attttgaagg gaaagaaaca ttggaagaag caacagaaca
    23821 agtcaaacta cattcgagaa gatacgcgaa acgtcagttg acgtggtttc gtaaccgttt
    23881 ggatgcacag tggtttgatc ttataagtga gccaaatcaa aaaagtcaga ttgagcaaac
    23941 aatcaaaaaa tggctggagg cggaataaaa tggaagaaaa agtgatcatc gtaggggtag
    24001 aaaccgaagc aaaccagcgt tacttttcag agtcaatgga agagctagtt cagctgacga
    24061 ataccgcatc aggagaagtc gtattcacaa ttacacaaaa gagaccacaa gtggatcggc
    24121 aaacgattat cggtaaaggg aaattacaag aattagttca acaagcggat gcgcatgaag
    24181 cggatttgat catctttaat catgagctaa ctccgagaca gagccaactg attaccgatg
    24241 ctgttggtct tccggtaatt gatcgcgtgc agcttatctt agatattttt gcgatgcgtg
    24301 ctcgttcgaa agaagggaag ctgcaggttg aacttgctca gttagaatac ctgctgcctc
    24361 gtttagtagg gcaagggaaa acattatctc gtcttggcgg tgggatcgga acaagagggc
    24421 caggtgaaac aaagttagaa acagaccgcc gacatatacg gaataaaatt tttgctgtta
    24481 agaaagaatt gaaagaagta gaagctcatc gagagcgcaa tcgccaaaaa agaaaaaata
    24541 gcgagatttt tcagattgga cttattggct acacgaatgc cgggaagtca actatcttga
    24601 atctattgac acaagcggat acttattcga aagatcaatt gtttgcaacg ttagatccat
    24661 tgactaagag atggcgtttt gctgaaggat ttgagattac tgtaacggat accgtcgggt
    24721 tcatccaaga cctgcctacg caactgatcg atgctttcca ttctacatta gaagaaagtc
    24781 aaaatatgga tcttttgcta catgtagtcg atgctagttc tccagatcgt attttacagg
    24841 agcagactgt cttaaaattg atggatgaac tgaatatgaa agaaatgccg atactgactg
    24901 tttacaataa ggcagaccag atcgacccag cgatgttcac gcctacgcta ttcccaaatg
    24961 tattgatttc tactcagacg aaagaaggta agatggcgtt gatcgaagca atcaaacgtc
    25021 agctgatgga actaatgacc ccttatacac tttctgtgcc aagcaacgaa ggacagaaat
    25081 taagcgagtt gagaagacaa acaatggttt tggacgaagc gtacatggaa gaaacaaacg
    25141 aatatgaagt taaagggttt gcaaaaacca attcaaaatg gttaagaaaa aaccaataaa
    25201 attttatttt tttaa
//
LOCUS       DAYYFU010000035        24575 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000035 DAYYFU010000000
VERSION     DAYYFU010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.035"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(152..550)
                     /locus_tag="ACHA34_001699"
     CDS             complement(152..550)
                     /locus_tag="ACHA34_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFM8792317.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="ACHA34_001700"
     CDS             complement(547..1320)
                     /locus_tag="ACHA34_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8792318.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="ACHA34_001701"
     CDS             complement(1317..2000)
                     /locus_tag="ACHA34_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8792319.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="ACHA34_001702"
     CDS             complement(2167..3054)
                     /locus_tag="ACHA34_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8792320.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="ACHA34_001703"
     CDS             complement(3051..3920)
                     /locus_tag="ACHA34_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8792321.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="ACHA34_001704"
     CDS             complement(4057..5343)
                     /locus_tag="ACHA34_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8792322.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="ACHA34_001705"
     CDS             5616..6194
                     /locus_tag="ACHA34_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8792323.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="ACHA34_001706"
     CDS             6194..7123
                     /locus_tag="ACHA34_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8792324.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="ACHA34_001707"
     CDS             7120..8856
                     /locus_tag="ACHA34_001707"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8792325.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ACHA34_001708"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ACHA34_001708"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFM8792326.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="ACHA34_001709"
     CDS             complement(9752..10261)
                     /locus_tag="ACHA34_001709"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFM8792327.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ACHA34_001710"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ACHA34_001710"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM8792328.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="ACHA34_001711"
     CDS             complement(11562..12653)
                     /locus_tag="ACHA34_001711"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFM8792329.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ACHA34_001712"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ACHA34_001712"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFM8792330.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ACHA34_001713"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ACHA34_001713"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFM8792331.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ACHA34_001714"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ACHA34_001714"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFM8792332.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16616..17590)
                     /locus_tag="ACHA34_001715"
     CDS             complement(16616..17590)
                     /locus_tag="ACHA34_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFM8792333.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17571..19454)
                     /locus_tag="ACHA34_001716"
     CDS             complement(17571..19454)
                     /locus_tag="ACHA34_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFM8792334.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19472..21697)
                     /locus_tag="ACHA34_001717"
     CDS             complement(19472..21697)
                     /locus_tag="ACHA34_001717"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8792335.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21812..22846
                     /locus_tag="ACHA34_001718"
     CDS             21812..22846
                     /locus_tag="ACHA34_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8792336.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22913..23926)
                     /locus_tag="ACHA34_001719"
     CDS             complement(22913..23926)
                     /locus_tag="ACHA34_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8792337.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24004..24192)
                     /locus_tag="ACHA34_001720"
     CDS             complement(24004..24192)
                     /locus_tag="ACHA34_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFM8792338.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7061 a         5341 c         4397 g         7776 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tgcaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccga tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt
    16441 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattggt tttattgaag
    16501 aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag
    16561 aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact
    16621 gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat
    16681 ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg
    16741 ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga
    16801 atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt
    16861 cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt
    16921 ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt
    16981 tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt
    17041 catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt
    17101 ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga
    17161 tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga
    17221 tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca
    17281 aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg
    17341 ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg
    17401 ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg
    17461 attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta
    17521 atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg
    17581 tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa
    17641 acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag
    17701 cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc
    17761 catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt
    17821 ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct
    17881 ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct
    17941 gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg
    18001 gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca
    18061 tttccaagag accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt
    18121 gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc
    18181 tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca
    18241 tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc
    18301 cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat atcttctata
    18361 gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc
    18421 cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa
    18481 tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat
    18541 aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc
    18601 atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc
    18661 gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg
    18721 ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt
    18781 cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct
    18841 tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc
    18901 aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa
    18961 tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt
    19021 tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct
    19081 ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg
    19141 cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt
    19201 gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt
    19261 ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca
    19321 tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca
    19381 tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt
    19441 atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg
    19501 tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg
    19561 aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat
    19621 atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt
    19681 catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca
    19741 aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt
    19801 cttttacttg ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa
    19861 cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat
    19921 ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat
    19981 attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat
    20041 aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt
    20101 ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg
    20161 ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt
    20221 tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct
    20281 caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag
    20341 aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat
    20401 caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat
    20461 aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta
    20521 atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga
    20581 tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt
    20641 aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg
    20701 cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg
    20761 agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa
    20821 aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt
    20881 cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg
    20941 tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac
    21001 ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga
    21061 catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct
    21121 ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag
    21181 atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg
    21241 tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag
    21301 tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca
    21361 gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca
    21421 tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat
    21481 ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt
    21541 actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta
    21601 tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga
    21661 tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga
    21721 aaccgtttac ttttttttat aattcctgta tactgtttac taaacaattt tgtcaataaa
    21781 aattttttat ttttttgagg agtgtgtaaa aatggttgga attagagatg tcgcacgaaa
    21841 ggcaaatgtc tctcctgcta cagtttcccg tgtgttgaat aacgatcaga cgatccatgt
    21901 ggctcagact actcggcaac ggatagtaga ggcagctgct gcgttggatt atgacatcag
    21961 tactcgtaaa tatatcaaaa agagaatgcc ttcgattggt gtgatctcta cgatcggcaa
    22021 agcgagcgaa gaacaggata tctattacaa acatttgcga gtagggatgg aagaagaagc
    22081 tcgacgtctg catttaggga tgaatcgaat ctataatcta tctgataatc ctaaacaatg
    22141 ggaagatcta gatcagttag gtgcggtgat cgttgttggg actgttacga aagaatcgat
    22201 taccgccttg ttaaaacaaa acatgcaaat agtcgtcgtg gataaccctg acatccagca
    22261 agaggtcgat atggtgtatg cagatcttga acggatgact aaaaccgtac tggaattgtt
    22321 cttagaaaat ggtcataaaa gaatagccta tatcggggga tataaagtcg atatggatga
    22381 attcggtggg aagcagattt ctataaatga aaagaggatg cgtgcctatc agcattttat
    22441 gatcgatcat cacttagaaa aatatatgtc ttatcatcta ggtggctgga cagaaaaaca
    22501 aggtgaggta cttgcgacta atctattaga aaatgaatcc cctcttccta ctgccattct
    22561 tgtcgggagc gatccaatgg caattggtgt ctatcgggca ttgaaacaaa aaaatatcca
    22621 aattggagaa gagattgttg tagcaagctt tgataatatc gagacaatcg caaacctctc
    22681 ccctggcttg accagtgttc aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat
    22741 ggcattggag cgaattgacg gtatacggga cgaaccaatc attttgactt atcctgcacg
    22801 attgattgtt agggaaagtt ttatcccaag aacaaaaagg tattgaaaga gaggttgtga
    22861 aaagccgctt cgtttcttca tactcaaaac gaaacggctt tttcacaacc tcttacttat
    22921 acttattcac aaaatccaat gtctgctggt aacaattttg cgattctttt agcgtgtaat
    22981 caaattggta ttcatgggta atctcttttt ttgtatctgt ataaaacaag gattgcacgg
    23041 gtacctttaa ttctgtcaac cgattttcta atgcttgacc ttgctcctgg aatgagtatg
    23101 catttccgtc agttatatac gttggcggaa attccttaga aacatggtca gccacactag
    23161 cttctttcaa ttgctcactc gttttccaat cttttgttcc aatcaacgac cacgcaacgg
    23221 ttttgacgaa aaacttcata aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat
    23281 caacaggtcc ccaataagaa atggttcctt tcagactatc ttttggaagt agctgtttga
    23341 ttttcatttg tttagcatac gcatcattcg tttgtatagc tgcatattgc aatgcgattt
    23401 gtgaacctgc actatctcca ccaaaaaata attgttccat gtctagcaat ttgtcgtctt
    23461 tttcttcagc taataaatct ttgatcagtt cattcacttg aagaacttga ttagggtatt
    23521 ctgaatcagg cgctaattca taattcatcg caaccactgc gatgttggcg tctgaaacta
    23581 attttgtcgc aaattctttg acacccgatt tatctccgcc gacgaatcct ccaccatgta
    23641 cccaaaatag aacaggaaca ggccccttgc tgttttttgg atagtagatg tcataagtat
    23701 tctctttata cttggactca tacgtcaaat ctgttttttt cgccacgttt ttttctgctt
    23761 gatcgaacgt ttttttatca gtgatttgaa cagtatttga aaacatatgg ttgatcaaga
    23821 atgctccagg tctgggggaa agctggaagg caagaaagat tgcaaacgcg atcccaagaa
    23881 tgattccgaa gaaccatccg ataaatttaa gaattttctt tttcataagg ctccctcagt
    23941 agttagtatt aagcattctg attataccat agtgttattt cctatcaagc tctcctagat
    24001 gatttaatga ttttccccaa acttcttctg caatgctttt gacaaaagaa attttttcca
    24061 ttgttcttca aacaaaacat tttcttcttc tgttgaagca aatccacatt gaggacttaa
    24121 gcacaaacga tccaaaggca gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg
    24181 gtgctcttcc atttcgccat ttttcgttac agtcaaaatc taagacaact aatttatctt
    24241 cagagacttt ctctaaaaga acaaaactcc cagcacgttc actatcgtat tctaaataat
    24301 aggcacggac attttcttga gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg
    24361 ccgccaaaat cgaataataa tttccgcgtc aaacatgtat atttagtgtt gaatcatttg
    24421 agaatatgaa aataaaaatc tttttgatca atcagttaat caaattgaaa taacgaaaaa
    24481 tctgagacag aatgacgttc attctgaccc agatttgatt ttgcacaact caacagtcct
    24541 tattggcaaa tcgccaaaat aatgtagact aacta
//
LOCUS       DAYYFU010000036        24436 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000036 DAYYFU010000000
VERSION     DAYYFU010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.036"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..789)
                     /locus_tag="ACHA34_001721"
     CDS             complement(55..789)
                     /locus_tag="ACHA34_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8792339.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="ACHA34_001722"
     CDS             1116..1526
                     /locus_tag="ACHA34_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFM8792340.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="ACHA34_001723"
     CDS             1519..2220
                     /locus_tag="ACHA34_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFM8792341.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="ACHA34_001724"
     CDS             2220..3806
                     /locus_tag="ACHA34_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8792342.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="ACHA34_001725"
     CDS             3825..4046
                     /locus_tag="ACHA34_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFM8792343.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="ACHA34_001726"
     CDS             4043..4804
                     /locus_tag="ACHA34_001726"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFM8792344.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="ACHA34_001727"
     CDS             5184..5693
                     /locus_tag="ACHA34_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8792345.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="ACHA34_001728"
     CDS             complement(5768..6307)
                     /locus_tag="ACHA34_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFM8792346.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACHA34_001729"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACHA34_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFM8792347.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="ACHA34_001730"
     CDS             7320..8171
                     /locus_tag="ACHA34_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFM8792348.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="ACHA34_001731"
     CDS             8199..8834
                     /locus_tag="ACHA34_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFM8792349.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="ACHA34_001732"
     CDS             8854..9627
                     /locus_tag="ACHA34_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8792350.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="ACHA34_001733"
     CDS             10035..10832
                     /locus_tag="ACHA34_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFM8792351.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="ACHA34_001734"
     CDS             10810..11223
                     /locus_tag="ACHA34_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8792352.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="ACHA34_001735"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="ACHA34_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8792353.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="ACHA34_001736"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="ACHA34_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFM8792354.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="ACHA34_001737"
     CDS             16000..16560
                     /locus_tag="ACHA34_001737"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFM8792355.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACHA34_001738"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACHA34_001738"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFM8792356.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="ACHA34_001739"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="ACHA34_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFM8792357.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="ACHA34_001740"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="ACHA34_001740"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFM8792358.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ACHA34_001741"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ACHA34_001741"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFM8792359.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ACHA34_001742"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ACHA34_001742"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFM8792360.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ACHA34_001743"
     CDS             21907..22485
                     /locus_tag="ACHA34_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8792361.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ACHA34_001744"
     CDS             22537..23019
                     /locus_tag="ACHA34_001744"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFM8792362.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ACHA34_001745"
     CDS             23136..23510
                     /locus_tag="ACHA34_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792363.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            23940..>24436
                     /locus_tag="ACHA34_001746"
     CDS             23940..>24436
                     /locus_tag="ACHA34_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFM8792364.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT         8012 a         4391 c         5213 g         6820 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatagaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt ttgagagtat aaaatatttt gtgtaaatga aaaaatccat acaaaaaagg
    23881 aagtcccttc tgtagaataa agttaacgac aaccaattca cagaaaagag gacttcccta
    23941 tgaatgattt tactacagaa attgtgcaaa ctctagtcac taaaggcgat ttaaatgaat
    24001 tattccgttc gcacttagaa aaagcgataa acacactcct acggactgaa ttaacggctt
    24061 ttttagatta cgaaaaatat gatcgcactg gttttaattc aggtaattcg agaaacggtt
    24121 cttactttcg atcaatcaaa accgaatatg gtgaattaac attggaaata cctagagatc
    24181 gtaatggtga gtttaaacaa caaactttac cagcctacaa aagaacaaac gatacattgg
    24241 aaaccactat tatccattta ttcgaaaaag gtgttacgat gtctgaaatt gctgatttga
    24301 tcgaaaaaat gtacggtcat cactatactc cacaaaccat gtccaacatg actaaagttc
    24361 tgactgaaga agtaaatgcc tttaaatcca gagccttaaa tgataagtat gtcgctattt
    24421 ttatggacgc tactta
//
LOCUS       DAYYFU010000037        23261 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000037 DAYYFU010000000
VERSION     DAYYFU010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.037"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACHA34_001747"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACHA34_001747"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFM8792365.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACHA34_001748"
     CDS             complement(1172..1867)
                     /locus_tag="ACHA34_001748"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFM8792366.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACHA34_001749"
     CDS             complement(1880..2596)
                     /locus_tag="ACHA34_001749"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFM8792367.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="ACHA34_001750"
     CDS             2926..3534
                     /locus_tag="ACHA34_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFM8792368.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="ACHA34_001751"
     CDS             3859..4182
                     /locus_tag="ACHA34_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM8792369.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="ACHA34_001752"
     CDS             4364..5734
                     /locus_tag="ACHA34_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFM8792370.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="ACHA34_001753"
     CDS             complement(5864..7165)
                     /locus_tag="ACHA34_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8792371.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="ACHA34_001754"
     CDS             complement(7519..9510)
                     /locus_tag="ACHA34_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFM8792372.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="ACHA34_001755"
     CDS             complement(9654..9920)
                     /locus_tag="ACHA34_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8792373.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACHA34_001756"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACHA34_001756"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFM8792374.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACHA34_001757"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACHA34_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFM8792375.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /gene="def"
                     /locus_tag="ACHA34_001758"
     CDS             13264..13827
                     /gene="def"
                     /locus_tag="ACHA34_001758"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8792376.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="ACHA34_001759"
     CDS             complement(14080..14601)
                     /locus_tag="ACHA34_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFM8792377.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="ACHA34_001760"
     CDS             complement(14604..15446)
                     /locus_tag="ACHA34_001760"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFM8792378.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="ACHA34_001761"
     CDS             complement(15439..16164)
                     /locus_tag="ACHA34_001761"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFM8792379.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="ACHA34_001762"
     CDS             16340..16636
                     /locus_tag="ACHA34_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792380.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16892..17066
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17204..18796
                     /locus_tag="ACHA34_001763"
     CDS             17204..18796
                     /locus_tag="ACHA34_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8792381.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="ACHA34_001764"
     CDS             18809..19549
                     /locus_tag="ACHA34_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8792382.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="ACHA34_001765"
     CDS             19717..21066
                     /locus_tag="ACHA34_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8792383.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="ACHA34_001766"
     CDS             complement(21194..21784)
                     /locus_tag="ACHA34_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8792384.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            22020..23099
                     /locus_tag="ACHA34_001767"
     CDS             22020..23099
                     /locus_tag="ACHA34_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8792385.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT         6661 a         4820 c         4527 g         7253 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aaaggcggat tttataatta acttagccat ttggaaataa aaaaacgcca
    21961 cgtgaattcc atgttatatt gaagttacca caactctcaa tagacaagga tgaattcaca
    22021 tgacgcaaaa ccatgataac acagtttcac gcaagggaaa acacctaacc tactcagaac
    22081 gttgccaaat cgccatcctg aaaaaggaaa actattccaa ccgtcaggtt gcggatgtcc
    22141 tgggctgcgt gccgcagacc ataaacaacg aaatcaaacg cgggaccgtt actcagctta
    22201 accgccagaa tcagaacggg aaggtctacg actattatca tgacgtctac gacgccgatg
    22261 ccgggcaggc ggcctacgag aagaaccgct tgaactgcgg cagacggccc ttatgggccg
    22321 acacggacgc cttcattgaa tgggcggacg acatgatgct gttggaaaaa tggtccccgg
    22381 acgtggtggt gggctttgcc agaaaacacg aattgttcga cccggccatt atccctggga
    22441 ccaccaccct ctatcagtgg atcgaccgtg gcatcatgaa aacgaagaat atggacctgc
    22501 tagaaaaact gtcccgcaaa ccgaaatcgt cccgtcatgg caaccgcccg aataaacggg
    22561 tgctagggaa atccattgaa gagcgcccgg aggcggttgg tacccggaaa accttcggcc
    22621 attgggaagt cgacacggtc gtaggaaaca agaccaagtc ggatgccgtg ctgctgacgt
    22681 tggtggaacg gcagacacgc ttcgaggtca ttctgaagct ggccgggaag gatgccgaat
    22741 cggtcaacca agcggtccga cagctgagag aacgggctgg tgcgtacttc ggccagctgt
    22801 tcaagacggt tacctcggac aacggctcgg agttcgcggg actgcacgat gccttgcagg
    22861 atgtggcaaa catctacttc agccacccct atgcttcctg ggagcgcggg acgagcgaaa
    22921 accaacacaa gctgattcgc cgtttcatcc ccaagggcag cccaatcggc aacgtgagcg
    22981 aaaaccaaat cttgcggatt cagcgttgga tgaacgatta cccgcgaaag attttgggct
    23041 atgcaacgcc gcatgacacg ttcgtccagg catttaggca ggaacggttg acggcttgac
    23101 acgtgggccc ttgacaacga gcctccctcg gcatggcttg tgtctggaaa gctggccggg
    23161 gccagccagt cgggagacta ccatgcctca gctcattgtc aagggcccac tcgattagaa
    23221 aaattagacg gttggtggta acgagcaacc gggtcaccat g
//
LOCUS       DAYYFU010000038        23064 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000038 DAYYFU010000000
VERSION     DAYYFU010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.038"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(110..565)
                     /locus_tag="ACHA34_001768"
     CDS             complement(110..565)
                     /locus_tag="ACHA34_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792386.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(719..1927)
                     /locus_tag="ACHA34_001769"
     CDS             complement(719..1927)
                     /locus_tag="ACHA34_001769"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFM8792387.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2039..2371)
                     /locus_tag="ACHA34_001770"
     CDS             complement(2039..2371)
                     /locus_tag="ACHA34_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8792388.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2519..3394
                     /locus_tag="ACHA34_001771"
     CDS             2519..3394
                     /locus_tag="ACHA34_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8792389.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3476..4057)
                     /locus_tag="ACHA34_001772"
     CDS             complement(3476..4057)
                     /locus_tag="ACHA34_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFM8792390.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4063..5322)
                     /locus_tag="ACHA34_001773"
     CDS             complement(4063..5322)
                     /locus_tag="ACHA34_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8792391.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5324..6370)
                     /locus_tag="ACHA34_001774"
     CDS             complement(5324..6370)
                     /locus_tag="ACHA34_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792392.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6605..6880)
                     /locus_tag="ACHA34_001775"
     CDS             complement(6605..6880)
                     /locus_tag="ACHA34_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFM8792393.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7123..8433)
                     /gene="der"
                     /locus_tag="ACHA34_001776"
     CDS             complement(7123..8433)
                     /gene="der"
                     /locus_tag="ACHA34_001776"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFM8792394.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="ACHA34_001777"
     CDS             complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="ACHA34_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFM8792395.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9979..10659)
                     /gene="cmk"
                     /locus_tag="ACHA34_001778"
     CDS             complement(9979..10659)
                     /gene="cmk"
                     /locus_tag="ACHA34_001778"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFM8792396.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10708..11343)
                     /locus_tag="ACHA34_001779"
     CDS             complement(10708..11343)
                     /locus_tag="ACHA34_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFM8792397.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11409..12812)
                     /locus_tag="ACHA34_001780"
     CDS             complement(11409..12812)
                     /locus_tag="ACHA34_001780"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFM8792398.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12799..13830)
                     /locus_tag="ACHA34_001781"
     CDS             complement(12799..13830)
                     /locus_tag="ACHA34_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8792399.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13861..14082
                     /locus_tag="ACHA34_001782"
     CDS             13861..14082
                     /locus_tag="ACHA34_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFM8792400.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14247..14984)
                     /locus_tag="ACHA34_001783"
     CDS             complement(14247..14984)
                     /locus_tag="ACHA34_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFM8792401.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15137..16477)
                     /locus_tag="ACHA34_001784"
     CDS             complement(15137..16477)
                     /locus_tag="ACHA34_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8792402.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16714..18237)
                     /locus_tag="ACHA34_001785"
     CDS             complement(16714..18237)
                     /locus_tag="ACHA34_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM8792403.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18615..19226)
                     /locus_tag="ACHA34_001786"
     CDS             complement(18615..19226)
                     /locus_tag="ACHA34_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8792404.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19349..19474)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19556..20272)
                     /locus_tag="ACHA34_001787"
     CDS             complement(19556..20272)
                     /locus_tag="ACHA34_001787"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8792405.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20278..20847)
                     /gene="scpB"
                     /locus_tag="ACHA34_001788"
     CDS             complement(20278..20847)
                     /gene="scpB"
                     /locus_tag="ACHA34_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFM8792406.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20831..21601)
                     /locus_tag="ACHA34_001789"
     CDS             complement(20831..21601)
                     /locus_tag="ACHA34_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFM8792407.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21598..21969)
                     /locus_tag="ACHA34_001790"
     CDS             complement(21598..21969)
                     /locus_tag="ACHA34_001790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8792408.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21988..22875)
                     /gene="xerD"
                     /locus_tag="ACHA34_001791"
     CDS             complement(21988..22875)
                     /gene="xerD"
                     /locus_tag="ACHA34_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFM8792409.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6467 a         4926 c         3910 g         7761 t
ORIGIN      
        1 aaaatcttgt cacagtccct tattctgaat aaacggtgtg gaagagacag cctatcagta
       61 gtaataagac acatctcttt cacagcctca tttttgcgta aaaatcgatt tattcgaaaa
      121 attccccgta tacatcttct acacgttgat ctggattgat cacgatatac aagttacctg
      181 ttttttcgct gatttttgtt gattgatatt gattgtctaa tccttctgtt cctttatcta
      241 gatatgggaa gtacacaaga tcaggtgtgc catttcggta aaggatttcc attcgctcga
      301 tatcttcgta tttcaatgcg cgattaaaca tgcccatttc cagtccgcct aggttgatat
      361 catgtgtcgc aactgcgtcc ccttcatgga agatttcgat tttaaatcca gcacacggat
      421 ggatttcaac aaattcactg ccattgattc gtccaaaact tgttgtgatt tgtttgatcc
      481 aaagatcgcc gatatgacgg cggttgatcg tccaggtttc tccatttcta aaataaaatt
      541 ttagcgctgt catttcttta ctcattaaaa agactccttt atgatgtaat ttattcttgt
      601 actacctcag tttagcacaa atttatgtaa gtggtcacat cttatgtttt ttcttctaat
      661 gacgtatctg cccactttaa gtagccaaca aagctgagca gatacagtct agtgtttttt
      721 aaatcaattt ttctttcgat aaaaaatcca aaacctgttt tttatcattt tttattcttt
      781 gttctacgac ggctttttca gctaattgca aagcttcact aacccactta ccaggttttt
      841 ttccagtttc ttctagcaag attttaccat ctactgctaa ttcgcttaat gacttgatag
      901 gtaaacgatc ataggattct cctacggcat ccatctgagg tgtttcgtca aaatagatca
      961 tcaattcttc tacttgcagt gcatgttttt tgcctaagaa gtacaattcg aatggttcca
     1021 aagctccagt ttctcgttgt cgtagtcctt ggacaagtgc ttgaacgtct ttaatcattt
     1081 ggttagaaca cttccacgat ttcaaaaagg aacgaatctc attttctttt agatccagtt
     1141 ggctgatcaa taatgcccat gcttggctct cttctttcaa tgggacggat ggtaattcag
     1201 caaaacgtaa aagtgcttcg ccttcttctt tcaaaccggg acagcaaatg tagcactctg
     1261 tttcaataaa tgcatggatt cccgcggccc cttcatttcc caagagcatc ttgatgaatt
     1321 ccacatttat acgttctaca gatattttac ctaatagctc gtggttttct ttgatggcgt
     1381 agaaggtttc ttcctctaat ttgaacccta gttgactgac aaaacgcaac ccgcgcatca
     1441 ttcgtaaggc atcttcatgg aaccgctcat gaggattgcc tacagctcgc aagatctgtt
     1501 cttctagatc ttgcagacca tcgaacaaat cgatgatttc tccatcttct ttcaaagcaa
     1561 acgcattgat cgtaaaatct cttcttttca aatcttctgc caacgaacga acgaattcga
     1621 catgatctgg tctacggtag tcctgataag ttgattccgt ccgaaatgtg gtgatctcat
     1681 attgttcatc attttctaat accaacacag ttccatgttc gatcccaata tctatcgttt
     1741 ttggaaaaag ctgtttgatt tcttctggaa atgcgcttgt tgcgatatcc acatcatgga
     1801 ttggctggtt cagcaagata tcacgaacac ttccacctac aaaataagct tcatatcctg
     1861 cattctctaa ttttttcaga actggaattg ccttttggta ttccagaggt aattgtttta
     1921 atttcatttt tccaaccttt cctgcgtgtt tcttgtcctg agataaagac tcagttgcat
     1981 ggtgactgtt tgacaagtcc gtttcagagt cttcaaggcc aggaccactt catcttgttt
     2041 actcgtcctt acgctcaaag cgattatggt ctctttgatt gaatttttcc atgttttttt
     2101 caaaaacttc agttaagtcg atattcaatg aattagccat gatcatcgta acgaataaaa
     2161 catcaccaag ttcttctgct accgagttaa ctggttcaga agcctttttg cttttttctc
     2221 cataagtgtg gttgacttct cttgccaatt cacctacttc ttcagttaat cgagccatct
     2281 gagctaatgg agagaaatac cccaccttga attgctggat atattcatcc acttcctgct
     2341 gcatactgta taaagaacgc tttttatcca tctttttccc tccaaaacta tcttaccaaa
     2401 actttaacgc aaatcctaga taagagcaat tgagaaaatc gaatttccat gctacattta
     2461 ggaaggaatt tctaaatgga aattctgtga aaatacataa ttgaaagctg gaattcatat
     2521 ggtcaaacga aaaacagtaa aagaagttct ttttatcatt ataggaacaa gtatttatgc
     2581 atttggtctt gtttacttga acatcgctaa tcatttagca gaagggggag tctcagggat
     2641 caccttgatc ttaagagctt tatttggtat tgatccagct tatacgactt tattaatcaa
     2701 catcccattg attttactag gaggaaagat cctcggtaaa cgttccttgg cctatactgt
     2761 tctaggtaca ctttctttgt cattcttttt atggatctgg caaagggttc cactagaaat
     2821 caatttacag catgatctac tgatcgtttc tctacttgcg ggtttagttg caggtgtagg
     2881 aagcgggatc gtctaccgca tgggtggaac aacaggcggc agtgatatta tcgctcggat
     2941 tttagaacaa aattacggca tcactatggg gcgttcccta ttggcatttg atatcatcgt
     3001 cttacttgct tcattaacct atatcgactt gaaacgtatg atgtatacgt tgattgtttc
     3061 gtatgttttc agtcgtgtga ttgattcgat cttagatggc ggatattctg caaaaggtct
     3121 acttgtcgtc tccaacaaaa gcgaagagat tgcaccacta ctgatgaccg gtctgcaaag
     3181 aggtgtgact ttcctttctg gagaaggcgc attctctggt aattcaaaaa aaatcatcta
     3241 tatggtcgtt agctctagtg aattaactga agctaagagg atcgtccacg agattgacga
     3301 aaaagctttc ctttcaatca tcaatgtgca tgaagtggaa gggcaaggct ttacttattt
     3361 gaagccacag acaagattac tgaaaaaact ataagaaaaa agacaaagat aggctatgag
     3421 gaaagattcc acatccagct gacctttgtc ttttttagta ctatactatt tttaactaat
     3481 tgttatgttc acttacccgg ttacttttct tttgtgtctg caaaaggatc tttcggttaa
     3541 cttcatcaga taatttcaaa aaagcatccc tatctccatc ttctagtgct tgatcgattt
     3601 gagcataaag catttttagt tttgcctctt cttctttttc ttgaaaatat cgttcaatta
     3661 attcagtatc ttcttcactg actgaagaat cccaagaaga aaatggattg tcttctaaga
     3721 tagatagata ttctcttgtc tgccaagcat tttcgaatat acattccaca tataaaggct
     3781 ttttccaatt caaacggatt tcatgaaaaa tctggtctgt gtccgtaaat tcttttccct
     3841 gcaaaaacaa tttcatgggt tccccttcaa cagatagatc actgatctgc agtcctcgat
     3901 ccgcaaaatt cgccccttca acaaagtgga cattgcttaa aatcgcttca tgattggcaa
     3961 gataattcaa gatccaaaaa acttctcttc ggctaaaaga tacatgattg accatccacg
     4021 ttaaaaaatt cttcttttcc cgtacatcaa ttagcatttt tctcacctct ctgttaggtc
     4081 atctaataat gacagtgcct ccatatctcc tggttcaagt gttaaataat aagtcaataa
     4141 ttcttgcgct cttttcagct gcccttcttc tcttaagaaa aaggcatact ctttcaagaa
     4201 atctggttca tgtgatagtt catgataagc ctgttcatag tgtacagctg caacggtaaa
     4261 gtcttctaat tcattataag catgggctag gttccattct gcataaggat tagcagtttc
     4321 ttccatctga ttgatcactt gtatcacatc ttcatatctt tcatcatcca agtaaagatt
     4381 acttaaagta agtaatgttt catcttgttt gtctcctact tctaaagctt tcagtaaaag
     4441 ttcttctgct cgctgtctat catgcagacg gaaagcattt tccgaggcca ggtgataaag
     4501 ttcgacttgg taaggatttt ctttgattcc ttcttctaac actgtttgtg cttcttcaat
     4561 catttcttct tcttgaagag cttgtcctag atatagatat aacgattgat aatgcggatt
     4621 aacttctcga agttcttcca gatagttgat tgcctgctga ttttctttca attgaagatt
     4681 cgtaaaagct agctggaaca gtcgatcatc tgtcctttct tctttcagtg cttcttctaa
     4741 aggtacgata gctgcttcga attctccttg catacttaag gattgtccta aacgttcctg
     4801 catcgagatt cctgaaattt catttatccc gcttgccaac aatgtttcat aaaccactgc
     4861 tgcctctttg aaacgatcaa ctgaaaaata caattcagct agcgcaaact ggatcaatgg
     4921 ttcatcagga agcaacccag ctgcttcttt caattttgct tcactcactt cagggattcc
     4981 gataacttgg tacaaatcag ctatcgccaa taagctttgc ggatagtaat cactttgctt
     5041 atccactttt tccaggtatt caaaagctaa gtcgatctca ttatcttcga ttgcaatttc
     5101 tgctaacggg atgttcagtc cgtcattttc tgggtattgg ttgatcagtt gttcaaaaat
     5161 ctgttttgct tcgatcaaaa agccaatagc cagcaattct tcgcctagtt cagctaatat
     5221 atctgctggg tcttctttta atgctttttc taaaaagatt tgtgcttgtg acaagtcttc
     5281 atctgataaa gcttgcaaca ttttttcgct atttgtattc atttcaattc cctcttatct
     5341 cactgacgat ttgttgaaat accgtcattg ttctcaataa ttctttttcg tttggatgtt
     5401 ggtatccgtc tattatacct attttttcta aaacaagtat ttttttgctt ttttctgcca
     5461 aaaaaagaac tttttccaaa tactccatct ttgaaatctg ttccagccag tggattggat
     5521 agccaagcaa gtccagccac acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt
     5581 ggagattcca cacgaaatga aacatcattc ctaaaaattt tttcatacta gcagaaagta
     5641 gatggccatt ttcggtcaga taaaaagctt gttcaaacag tttaccataa tttgacaggt
     5701 tttctgcctc taattgataa aaatagatca tttcttcgat catcccggca aacggccgag
     5761 tcgttaattg ctttttattt ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac
     5821 aaatcattcc gcaacagata aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct
     5881 cgctgattgt ttgatcataa acgatgcgtt ctggcagatt gatggtttgc agcactacac
     5941 gatttggtag ctgcaaaatc gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg
     6001 atacagggat catccatagt gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca
     6061 gatccatgat cccttcattt ccaaatccga tgataaacat ggattgattt tcgggatagc
     6121 gtttgacgaa atcaagcagg ttctttaatt ctgttaaatg attgcattga gtgttggcac
     6181 aaatatacca gtcattgttt gcttgatttg caaataacgg gttgatctta tctgcaaaca
     6241 aatcataata gcgctgattc gttaaaaata agattggctg atcaatggaa agttctctta
     6301 gctgactgac tagcgtttct ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat
     6361 caagttccat acgcatccct cctcttctta tttttatttt accattagta agcaaattct
     6421 agaaaagata agagcaaagc tcacctcata acttggaaat aaataaaaat taaataaaat
     6481 acatgtgttt tctatgtatg acatacggat tcggaatctc tgtctcttat ttattccttt
     6541 tctgctagga aataaaaaga aaagcacttg tagccctaga gccacaagcg ctttttcaac
     6601 tgtcttattt aacagcgtct tttaatgctt tacctggttt gaatgcaggt actttgctag
     6661 ctggaatttc gatttcttca ccagtttgtg ggttacgacc tttgcgagct gcgcgttcgc
     6721 gaacttcaaa gttaccaaaa ccgattaatt gaactttttc gccgttagct aaagcatctt
     6781 gaatagttga aaatacagca tctactgctg cagttgcatc ttttttagtt aaatcagtag
     6841 ctgaagcaac tttttcgatt aattctgctt tgtttgccat ggatagattc acctcctcca
     6901 caagagatat gatagaaata tcatttagtt aaaactttct gagtggtcca gaagtttaaa
     6961 aagaaatatt gattaacttc aatattctgt tatcacgata tcatatgaac cgcttaatag
     7021 caaggttttt accaattttc acatgctttt tttagtcata gaaacgtaac aaacgaaact
     7081 gtctttttct tttgaaaaaa aaacgtcgat atggtaaaat gcctattttc tcctacgagg
     7141 gataattcgg atggacgtcc cttcaaaagt aaatgctttt ctgatttgat tttctaagaa
     7201 acgttcataa gaaaaatgca tcaattcctc ttcattgaca aaaacgacaa atgtaggtgg
     7261 tttgacagcg acttgtgtag cataaaaaat cttcaggcgt tttcctttat ctgttggtgt
     7321 tggattgatt gctacagcat ccataatgat atcattcaac acagccgaag agatacgaag
     7381 attctggttc atacttactt gttcgatcat ttcaggcaga cgttccaacc gttgtttagt
     7441 cactgcagaa acaaaaacga ttggagcgta atcaagatac cggaattcat cacgaatctc
     7501 ttgttcgaaa tcacgcattg tattggtttc tttctttaca agatcccatt tattcacgac
     7561 gataataatt ccacgaccag cttcatgggc gtaacccgct actcgcttgt cttgttcccg
     7621 aatcccttct tcagcattca gtaccatcaa aacgatatca gaacggtcaa tggcacgcat
     7681 tgcacgcatc acgctgtatt tttcagtatt ttcgtatact tttccgcgtt tacgcatacc
     7741 agctgtatct atcattaaga atttctgacc actttcagaa acgaagtggg tgtcgattgc
     7801 gtctcgggtc gttccttcaa tatccgaaac aattacacgt tcttctccta aaatcgcatt
     7861 gatcaaagag gatttaccaa catttgggcg accaatcaaa ctaaacttaa tcgtgtcgtc
     7921 gtcctcttct tcagtctctt cagagaaata cttcactgct tcatctaaaa tatcaccaat
     7981 ccctagtcca tggcttcctg aaacaggata aggatcgcct agccctaagg aataaaattc
     8041 atagatatcg tttctcattt ctggattatc tactttattg actgctaaaa tcactggttt
     8101 attacttctg tataaaattt tcgcaaccat ctcatctgcg tctgtcacgc cttcgcgtcc
     8161 gctgactaca caaatgatta catctgcttc ttcaatcgcg atttctgctt gatgcttgat
     8221 ctgatccata aaaggttcat cgccaagatc aattccgcct gtgtcgatca cgctgaattc
     8281 tcgacctaac cattcacctt ttgcataaat acgatcacga gttactccag gagtatcttc
     8341 gacaatcgag atacgctcac cagcgatgcg gttaaaaatc gtagacttac cgacgttcgg
     8401 gcggccaacg attgcaattg ttggatttgc catattattt cctcctcaca agtttccaat
     8461 ctcacttagt ttaacgaatg cattgccaac tttcaagcat agattgtcaa ttaaacgaag
     8521 tatttttatt ccttattgaa atactaatcg cttttcaaaa gaaaaactga gacaaaagca
     8581 gctgctttca tctcagtcac agatacaaaa tcaattattt ttcagaatca tcggttgctt
     8641 tttcttctac ttcttctttc aacgcatcac ctaaaatgtc tcccatagta aatccagtat
     8701 tttcttctgg taattcataa gactcaacat tttttggttc ttcttctgaa gctggttttt
     8761 cttctaaagc tttgatactc aatgcaatac gatgttcttc tggatggatc tctaatactt
     8821 tcactttcac ttcgtcacct tcatgaagca cttcatgtgg tgtcgcaata tgtttatgtg
     8881 aaatttgaga gatatggact agcccttcta ctccagggaa cacttcaaca aaggcaccga
     8941 agcttgtcaa acgtttaact gttccgtcta atactgtgcc aactggagct tttgtatcga
     9001 tttctgacca aggtccaggc tgtgtgtctt tgattgacaa ggaaacacgt tcttgttcag
     9061 gattgataga caacactttg acatttacat catcattgac agccaataca tcacttggtt
     9121 ttgctacatg actatgtgaa atctctgaga catggactag accgtcaatt ccgcctaggt
     9181 cgacaaatgc gccaaaatca gtaagacgtg caacacgtcc actgagaaca tctccctcat
     9241 ggatagttga aagaatttct tttttctttt cttctctttc gttttccact accgctttat
     9301 gcgataagat caaacgattt tctgatggtt cgatttcgat gattttaaag ctcaatgttt
     9361 tgcctttata gtctgaaaag tctgcaacaa aatgatcttc caccattgat gcagggacaa
     9421 aaccacgtac accgacgtca acgactaaac cgcctttgac aacatttgta acaggtgctt
     9481 cgatgacctt gccctcttgg aaatctcttt cgatatcttc ccaaactttt ttagcatcta
     9541 aacggcgttt tgaaagtagg tagctgccgt tttctttatc tttgccgatc gtactgataa
     9601 cgactaaatc taatacatca ccgactttta ctacttcgct gatatcttct accggcaaag
     9661 ttgataattc ttttgccgga acgactcctt caactccagc cccttcgatt ccgacaatca
     9721 cttgtttgtc ttcaatgact aaaacttcgc ccttaacaac atcgccaact tttacttctt
     9781 gtacgctttt cattgcgtct tccattgact ggttgttctc ttgattttga tcaaattctg
     9841 tcataacact tgtcctccta accaacattc tgccgtaagt acggtactgt ttttagttta
     9901 attgattatt caaaaaaaat aaagcgattt cgttcgtttt ccttgaaaaa acaattgttt
     9961 ttcatttttt ttcttaattt aaaaaagaag ataacctttt ttcaaaacaa cagcttttat
    10021 tgctgcaacg acttctggaa tcgatttgcc agtcgtatcg atacgaacag catcctctgc
    10081 ttgcttcagc ggagagacgt cccttgttga atcataatgg tctctttcct gtatctcttt
    10141 cgtcagtgtt tcaagatcag tcggaattcc tttttcttgg ttctctttga aacgtctttc
    10201 tgcacgctca cttacgcttg ctactaaaaa gattttcact tctgcattcg gtaatacagc
    10261 tgtcccgatg tctcttccgt ccatgacaac gccaccgcgt tcccctattt ttcgttggac
    10321 agcaactaat tcttctctga cttttccatg tgcagagact tcagaaacag cttttgtgac
    10381 atctggttga cggatttcta aagtcacatc ttttgatgct atgaatacat gctgtccatc
    10441 ttcttgttgc gaaaaagaga taggatactt tttgatcagc gaaactagcc cttcttcatc
    10501 tgaaaaagat acattgttcg taatagctaa ataagttact gcgcgataca tggcaccggt
    10561 atctgtataa atataattca attcttttgc taatatcttg gcaacggtac ttttcccaga
    10621 tgaagcaggc ccgtcgattg caatactgat ttgtgtcatg atataactcc tttttctcag
    10681 aaaaaaagcc gcaaagcgcg gctattatta tttaattctt aattcttgac cagggtaaag
    10741 cataaagttg tttggatcta agccattcaa ttcaaacaat tgatcagtgg tgatccctgc
    10801 acgctcagcc acttgttttg gaccttcgcc tgcttgaaca gtcgttgttg aaccggctgc
    10861 agctgatgaa gaaggtgttg tttctgctgc attgctgctt gaatcccctg cttgataagt
    10921 tgcctcactt gaggaagtcg gtgttgtctc tgctgcaata ctgctttctg acgttacacc
    10981 tgtatcttga gacacagtag attcttccgc gattgtacta gattctactg ttgaagtctc
    11041 agttgactcc tcttttgttg tacttgtaga actttctact actgatgtcg aagtttcaga
    11101 agaagttgct gctgttgcat tgttgcttga tccatttctg atccagaaat aagcacctgc
    11161 tggaatagcg atacataaga ccagtaaaat caaaaatagc gaaagaaata atgtatttcc
    11221 tttcttttgt tgtctctgag aacttcttga tgaaacttca tcatctgtat cgtagattgg
    11281 ttgttcccat ggttcttgtg tttcgttatt ttctgtagat tgatgtctat cttttctgct
    11341 cactgtttta cctcctaaac tctatcgtgt gtattatacc ataggaaaaa gtgtcttgtc
    11401 ctaaaaaatc aatgttccat gtttttttta aataaattta gtaaaatcgt ttcccaatga
    11461 agttgttcag tcttagaaaa aagcttcctc tttggcagaa gttctgcttt tgctccatca
    11521 atatcacaac aattctgcgg cttttcggag agtttttcgc caaaataagc taagatgaat
    11581 tttctgcggc agttttcttc attaatatag cgaagcatct gctgcaggcg gaattctttt
    11641 tctttttcct gacgtttgag ccgttccacc caattttctg gtttttcaga ctgctggatc
    11701 agctccatcc attttttttg cagctcatca aagggagctt gctttgtggc atattccaaa
    11761 taaagctcaa aactttgtcg ctgttctcta gaaagttgat taaagaaata atggatacgt
    11821 tcatccccac tttgataaaa aagaattgct gcactttctt cctgatctct gcctgctcga
    11881 ccgatttctt gtacatagtt ttctagactg tctggcaaat cataatggac cacataacgg
    11941 atatcagatt tatcgatccc cataccaaat gcattcgtag caatgagaaa ctgcagctga
    12001 ttttttacaa actgctgttg aagttgtctt ctttgagctg catcaagacc accgtgatag
    12061 tatccaactg taaaacgttc cctgaataaa tgatacaatc gttcgacttc tttttttgtt
    12121 gcacaataaa tgattgccgc tccttgtgct ctttccatga agtgttcaag ttcttgttct
    12181 ttttgctgcg ttttccgaac aaaaagcgag atattttgcc gattgactga ttgtctgatc
    12241 tcttttggtg gtcgatcaaa tagtacatga cgtatatctc ttgctactaa atctgttgct
    12301 gtcgccgtta gtgccagtgt gaccgggttt cctaaaagct ttcggatttt tcctaactgt
    12361 tgatattctg gacggaaatc aactccccat tgagacacgc aatgagcctc atctactaca
    12421 tataacgcaa tattttgttt tttcagatgc tctaaaagag aaggattagt caacatttcg
    12481 ggactcaaaa acaaaaattt atattgcgat agatgacgga ggacgtatct tctttcagat
    12541 tgcgaaagta aactattgaa agcagctact cgtttctccc ctcgtttttg taattgcgtc
    12601 acttgatctt ccattagaga aagcagcggt gacacgatga tgaccagacc ttccattaaa
    12661 taccccgtca attgatagca aaggcttttt cccgttcctg taggcaaaat agcgagagta
    12721 tcttctccat caagcaacgc ctgaataatt tcttcttggc cgggacgaaa tgaatcaaat
    12781 ccaaaatatt tttttaattc atttattaac attctgtatc gcttcctttc tcaatagata
    12841 gatttgatac aaacgaaatt ctccataatc taattgtcca tctacattcc attcgctaaa
    12901 gcgccactct cgcacggaat ccttatttgg ttccaaacgt tcgattgttt ctgcagagag
    12961 aatccactcg tatggaaaat catcaaaaaa caaggcccac tcaataagat gatctgttac
    13021 tgttcctctt tttaaatgac ggattgccat gatctggtca attgtctgac cattcataaa
    13081 catctgcttg gtgatcatca tgctttgatt gaaattttgc tgtaataatg aatcgatcaa
    13141 agcgtgccaa agcgaatctg gccgtttttc gatttgtgct aacaaaaggt ggatacatct
    13201 gctttgttgc agataaacct ctgactcatc attgtttttt gccagctgat aaggaagcag
    13261 ccctgttcgc tcatttccag ataattggtt agctaaaaaa tctgctgctt cttctggtat
    13321 ctccgaaaac agctgcacca aactctcata cgcagtatca atcaaaattt cacgtggcaa
    13381 ttggctttca ctaagccatt tttttagttg gaaagtataa aaaggacttg tctctgctgg
    13441 aagatagtct tgaatacccg ttgccagatt agaaattact tgaacagcaa atttaatgag
    13501 ccgccaacta gtttcccctg ttctgccata gcggtcaaaa cgcaaaccag tatgttctat
    13561 ttgatgttcg gacaacatat cagcccctgc aggcgttagc tttgcttcat tttcatttat
    13621 ttcgatccaa ccttgctgcg caattttttg caaaatttga taaaacttat cctgcttcaa
    13681 ttctggaaaa ctgttatgga taaacaaaag ttcgtgggta aatccataaa taagtactga
    13741 agtcgttcgt tttcccacaa ggagttggta aagagaacta atccgcagct tgtgtcctga
    13801 tgaaaatagc gataaaataa aaacaatcat attattctcc ttaagttagg atggttagaa
    13861 atgtcactac tatgtaaact tgtacctgaa cgctgtattg catgcggtct ttgtcaaatc
    13921 gaagcgccag atattttcga ttatgatgaa gaaggaatcg ttttatttgc ccaagaacca
    13981 gaggctcatc aacagttcgt gatgccggaa gaagaaacat atgtaaaaaa agcgtatcaa
    14041 aaatgtccag ttcgagcgat tttattagaa aataaaatat aaatataaac gaaactttaa
    14101 aaacgtacac taagttctct aaacttgaaa ataagccggg atctgtttct cttgttccgt
    14161 gaaatcccga cttattttga aagattcatt tgctatctat agttaattga ctagtttttt
    14221 cacggaccct ataggaccca tttatattat ttcaactttt tcagccagtt ttccaatgta
    14281 ttgacgatca cagtttgctg gtctgcgtta gaaatttctg cctctccatc tcctttttgc
    14341 tcaccatagc tgccaaaacc tccatgattt ccgccattga ttgatacata ctctgttctt
    14401 tccggtaaat actgcttagc ttcttgataa gcttcctgat tcaatacttt atcttttgtc
    14461 gccgtgatcg ataaaacagg aacagctgat tctgtcaatg ttcctttttt gtcgggataa
    14521 cttgccatga agaatacccc ttttaaatgt ttgtctgaat gattcttatt gacataacga
    14581 cttgccatta cgccacctaa tgaatggcca ccgatcacat aatcggaatt tggaaaatct
    14641 tctttgacct tatccgcttt gtttccgccc atcactgcta gatcaagtgg cattttgaca
    14701 atagcgactg aaaagccgtt ttttgctaaa tcttgcgccc aaatactata actttctggc
    14761 tctacgagag caccaggata aaaaataagt aatggagact ttccatgatt ctttttctct
    14821 ccttcaaaaa ataagtaatc ttctttgttt tttccttctt ttaccaacac ttgcgcttct
    14881 gacgatggtt cgtacgtatt ttgctgtata tagtaataac cggctccacc gagtaatcct
    14941 aaaatgatga ctaagccaat tagtattcgt ctaaatattt tcactttctc ccaccttttc
    15001 tatctgagtg accccttttt cttaatcaag tatgattata ccttaaaaaa ataagagaga
    15061 aatgcaaact ttccagcatc aaataaaaaa agacggaata tccatttgaa tatccgccct
    15121 ttttgtatgt ttatttttat ttagcacata aacgatacac agcttctgca taaatttcca
    15181 ttgctttata catactactt aattcccatc gttcattcgc ctggtgcata aagtcaggag
    15241 tatcagggaa catcgcaccg aatgccacac actggttcat cgtacgagca aaagtagcgc
    15301 cgcctgatac aaacggttca gtcatgtctc cagtaatatc gcgatatgtc cccaatagat
    15361 ttttcactaa ctcactgtct agtggcacgt ataatgaatc aaggaagtca aattcttcat
    15421 aagtcaaccc atatttttct gctgttttag ttaatttagc tactaaatca tctttcttga
    15481 atgtaacagg gattctcata tctacaccga tttttgtttg ttctggtgtg atttccaaac
    15541 ttgcaaagtt catcgttagc tcgccggatt gttcatcttc tgttttgccc acgacagctt
    15601 caccagttgc attttcttga actaattttc ctaagaaatt gattgggcca aaatcaaata
    15661 cttctgaaag agcaattgcc agacgagaaa tagcattcac tccttgtgct gcatctttcg
    15721 cgtggatact tttaccaaga acgacgatgc cttcaccttg ttcttcgaaa tcgaattcgt
    15781 gttttttcaa tgcttctttt acttcagaaa gcttttctcc agagtaagga gctgcatcag
    15841 gaacaacatt caacgctcct ccagctttta cagaaaactc gttcgttcct ggtcctgtca
    15901 agtaagcttg caataagcct ttttctgcat aaattaaagg aaattcagca tctggcgcaa
    15961 atccttgcgt aattccttct tctttttcgt tatatttttc taaacaacgc cataaatttt
    16021 cttcatctgt accaaagata aaacgaatac gtgtcttgaa ttccacgcca gcatcaatca
    16081 aagctttgac ggcatacatt gccgcaatag aaggaccttt gtcatcttgg gatccgcgac
    16141 caactaacca gccgtctttt atagttggat cgaatggttt tgtttcccaa ttattttcgt
    16201 cccctgctgg aacaacgtcc atatgacaca agataccgaa taattcatct ccagagccaa
    16261 tctctgaata accgtaatag ccttctggat ctttaaatgt tctaaatcct agcttttcac
    16321 tgatttccaa tactttatcc aaagcttcaa tcactttttt accgaaggga tgtccttgtc
    16381 ctgaatctgc ttcatctagt acggaaggaa ttcggatcaa ttcgctcaat gattctaatg
    16441 cttgttcatg atgttgttct gtcacaaatt ttttcatgat attatcctcc taatatgtta
    16501 aaaagggtca tgtcaaattc ctccgacaca acctcttctt ctcaaataat gacgttttaa
    16561 gcctgctcca tcagcaagca gaatgttctt aacaaatgat gaaagaaaaa acactcactg
    16621 aatggagcta ttgacttgtc cacgtgctta tcgatagccg tttatcggtt tcttcattct
    16681 tatgatagac cttgtacctg tatttgtcta ggtttagaac attgctgcta cacctaggat
    16741 cacacagatg gctacaaata atatcgcaat taatttaccg gtaaatttcc accacactga
    16801 aacatcgaaa cgagcaattg ctacagcacc cattacgata gcagatgttg gtgtaattaa
    16861 gtttactaaa ccagatgcag attgataagc agtgattact aaatccttac cgacgccggc
    16921 aaattcaccc ataggtccca tgattcccat tgttgcagca gctaagccgg aagttgaagg
    16981 aatcaagaat gacataggga tatagaaaat aaatgtaata atgatgaata cacttgaaga
    17041 taatgaactt aatccttgtt cgccccaatg taagatggtg tctgtgatta atccattgtt
    17101 cataaccact tggataccac gagcaacagc tactacgatt gctacaccga tcaaatcaga
    17161 agcaccagct aaaaattcag agacaaattc agactctttc atgccataag tgattgctac
    17221 tacgatggac attactaaga ataacatagt aatttctggg aagtaccagt cacctaatgg
    17281 aagcatgctt ttacctagaa ccgtacctag taccggaagg tttgttaacc agtttgtcaa
    17341 tgattcaaaa atcgtgaaat tcgcattcaa ggttgtccaa ggaatcaaac tgatgatcat
    17401 gataccaaaa gtaataaaga acaatacatt aacatgtttt tgttttttcg tgattacttc
    17461 ttgacgaccg attgcttcaa tatcgaaatg cttcatatct tctttacgtt ggttgtatac
    17521 taatgatttt gttggatctt tttcgatctt tgatgcataa cgatacacat atacaataga
    17581 aatagctaac aaaataacga acatcaagaa tcgaagacca ataccttcac ccatcgaaat
    17641 cccaacagct tgtgaagcta cgcctgttgc aaaaggattt accgttgaag ctagacagcc
    17701 tacttgagac cctactaata cgattgctac agctactact gtatcaaagc ctacagccat
    17761 catgactggg atcagtaaag catagaatgg aatcgtttct tctgccattc cgtaagtaga
    17821 gccgcctaat gcaaataaca gcatcaatac tggaataagc attttttctt ttcctttgaa
    17881 tttcttcaca actgaagcaa tcccggcatc tagtgcccct gttttgttga taatacctaa
    17941 aaatccacca acaaccaaaa tgaagaaaga gattgggatt gcagcaggtg tttctttagt
    18001 tagctccccc gctggtacac ctagcatccc attgattggc gccatgaaaa tatcccacag
    18061 cccttgagga ttttgttgta ctcgttcata tgatccagcg acgatgttgc ctgcatcgtc
    18121 tacactatag tgcccagctg gtataaacca agtaagtaca gcgattactg caataataat
    18181 aaataagacc gtatacgctg aaggcatttg aaattttttc ttttttgttt gttccatctt
    18241 cttcttcctt tcttttccgt tagagacaac aattttggct ctaatgttat tttatcaaaa
    18301 agagtatcag aacaaaaata ataacacgca ttttgtaaaa cttttgttca aaaagttcgt
    18361 ttttccctat tccctcctct tttttcagtt ttttaaagct ttcttctttt tcattgtagc
    18421 gtttcctttt ttttataaac aaacattttc gtcttttaaa aggagaagaa tttcacattt
    18481 gcaaagaatt gattgatttt tatgcataaa tcaaaaaaat actcaaaaca aacaaataaa
    18541 acgtttacat tattgcatgt cttgtatttt tttccaaaaa aaaagaccaa gaaatttctt
    18601 gatctttttg atatttattt gactgctgta tgacgttgtt cgaactgatg ttgttttcga
    18661 cttaaccatg gaagaagttg cgcatgcagc actaaaaaga cagcactcac gatgaatcct
    18721 ttgatcaagt tgaaaggaac gatcccaatc agtacatagt ttgctaaaga catgcccagc
    18781 atctgtgttg cgttcagccc taaaaacatt aagtaaagag gcgtaataac gaaataattt
    18841 gcgatactca taaaaatagt tagagcgatg gttcctgtaa tgacacctaa aaatttgttt
    18901 cttcctttat gctgtttctt tacactaaag aaataataaa tcgggaatgt aaagatcgtt
    18961 gttgataaga aactagcagt gtctcctacc aaattatccg gagaaaaacc tgtagtcagc
    19021 agatgtaata ctgaacgcaa gaacgctgtt gcaactccgg ccgctggacc gaataagaac
    19081 atgctgatca ataccgagat atcactaaaa tcgatcttca aaaaactaaa agccggcatg
    19141 atcggaaaag caaaatactg taaaactaat gcgatcgcag caaacatcgc tactgcaacc
    19201 attttctgta cacgggtgtt cttcatttga aatcctcctg ttaggactcc tcttcagcga
    19261 actttttgac gtttctctcc aaatgaaaaa actctatttt ccagggaaaa tagagttaga
    19321 gttatttgat caagcgtcgt caaataagct ctatcttctt tatccagact atactgtcgg
    19381 tatcggaatt tcaccgattc agctattttt ttgcaaaata ggtcgtggac tataaccacc
    19441 ggtcgggaat ttcaccctgc ccctgaagac gaaccttata taatttttgt ttacatattc
    19501 attatacata tctattcaaa aaacgcaaca tctaacaatt ataaaaagta agtgttcatt
    19561 ttgagtggtt cagcaaactg ctgacttctt ttttgttcag ttcacggtaa tccccaggac
    19621 gtaggccttg taaagtcaga tctccgtatt tttcacgttt tagtttttgc acaggaagac
    19681 caactgcttg aagcatattt ttcacttgat gattacgtcc ttcgtggatc gtcaattcca
    19741 cgatactgtg gtttgtctta ggatccactg ataaaatttg gaatttagct ggagatgttt
    19801 ttcgtccttc gatctttatt ccttttgtca aaggtgcaag catcgatttt gtagcgatcc
    19861 cttttacctt tgcaacgtaa actttatcca cttcatgttt tggatgtgtc aggcgctgag
    19921 caaaatctcc atcatttgtc agtagcaaga tccccgaagt atcgtaatcc agtcgaccta
    19981 caggatagat acgttctttt accatcggca aaagatccgt aacaactttt cttcctttgt
    20041 catctgcaac agcagaaata actcctttag gtttgtacaa taaatagtaa ccgtattctt
    20101 cttgataaat tgggacttca tccacttcca cagtgtcatg tttcccaact tttatcccta
    20161 attcccttac aacttgtccg tttactttta cacgtcctgc aaggatgaat tcttctgctt
    20221 ttcgacgaga agtgatgcca gcgtgggcaa ttactttctg caatctctcc attacaatta
    20281 ttctccttta ttcattccat caaagaatag atctggtgac acgtcttctt caagtgaaat
    20341 ttccatttct tgtgtatcag gcagttcttc taatgatttc aaaccaaaat aatctaagaa
    20401 gtagccagtt gttccatata atatcggccg acctggtcct tctactctac ctttttcttc
    20461 aatcagttta tgggcagcta gacgctgtac cgctcctgaa acctgaactc ctcgaatctg
    20521 ctcgatttca gctcttgtga ttggctgttt ataagcgatg attgctaagg tttcgacggc
    20581 ggcttgagat aatgtgtttg atgtcccttg cgcatatcgc ttaagtaagg gagccagctc
    20641 ttttttagtc gttaatacat aatggctttc cgtttcaaaa atatgcaaag ctgagtgcga
    20701 gttttgagtg taagattctt ttaattcttt cactaaactt gtgacttctg cttcgctgag
    20761 atttaacagt ccgcttaatt cttcattact gatcccttcc tcgccaacaa taaaaagaag
    20821 ggcttcaagt tcacttaatt tcgtcatgtt cggtatctcc tactgctgga taaagcataa
    20881 ttgattcata gttttctttt tgttgcgcaa tgatctgccc ttttttcatc aattctagca
    20941 aagccataaa agtagtgaca acttcacttt tagaaggtga ctcaaaaaaa gaatcgaaag
    21001 tacagccatg cgcatttctt tttatacgct cttcgataaa taccattcgt tcttcgatcg
    21061 ttgtgtcatc gggcgtgatc gttttttcca ctttttgaga aaatttctta cgttctaaga
    21121 ttttctgcat ggcacggatc aaatcgattt ttttgacttg tcctcgcttc aacggctttt
    21181 cagcatccgc taacccatcg atgttcatag gttcttttgt gtaataaaga cttcgttcct
    21241 ctgctttttc tgtcagtctt tcagctgcat atttatattt acggtattct aaaagctgag
    21301 tgaccaattc ttctcgagga tcttcttccc ataattcttc atcgtctgtg ccgaattctt
    21361 ggacaggaag caatgtcttg cttttgattg ccatcaatgt agcagccatc actaagtatt
    21421 ctcctgcaac agctagctcg agtgttttca ttgtatggat ataattcata tattgctccg
    21481 tgatcgtagc gatcggtata tcatatatat cgatttccat ttgctgaatc agatgaagca
    21541 gcagatcgag aggaccctcg aatacatcta atttaatatt gatctccgtc aactcattca
    21601 ctctttcttc tttttctttg ctagccattt tccaatgtaa ggctctgtga tttcagttgc
    21661 acggatggag acatccgggt acttttcctg taattcatct aacatttgaa agcccatatt
    21721 ctcaccgcga taagacggat tcaaagaaat gtcatggatg atcagctgat cgttcgttgc
    21781 ccagtaaata ccaataatcc cttgaatgtt ggttgaacct tctggcgtaa agaaaaaaag
    21841 ttcataatcg tcttcagatt cgtacgtatc aatctccttc aacaaagaag ttttacctgt
    21901 cagtttctta tggaaactca atagtcccat agcaatttta cggttttctt tccggtaatg
    21961 tgttaacatc ttctcacctt cctatgacta agcccttgga aaatgctgct tataaacatc
    22021 cgtcattctt ttcttcgtga tatgtgtata aatctgagtc gtagagatat ccgcgtgacc
    22081 taacaattcc tgtactgtcc gcaaatcagc tccattttct agcagatgtg tggcaaagct
    22141 atgccgcagc gtatgtggtg ttacttcttt gtaaatccca gcctctcgta ctaactgctt
    22201 cagatttttc cagattcctt gccgagaaag acctttccca tgattattga caaatacatg
    22261 catttcagat gcgtccttta caagtaatgg acgtgcttcg tctaagtagc gttccagcca
    22321 ttggatcgca taatcaccta aaggaatgat ccgctcttta tctccttttc caactgtctg
    22381 taccagtcca agagaaagat gaagatctcc caatttcaat ccaatcaatt cactgacgcg
    22441 catcccagta gcatacatga cttccaagat tgcacgatct cttatcccta acgtttttgt
    22501 cgtatcaggt gcttcaatca gcttttctac ttctgtcaga gtcaatgtac ttggtaattt
    22561 ctgtgctttc ttcggcgtat cgatatgttg catcggatca tgatcagtga tacgctcttg
    22621 ccgtaaaaac tgatggaatc ttctgagact tgagatcatc cgagtaacag ttgctgtcgc
    22681 ttttttttct tcatgcaact tctccagaaa acggatgact aaaaagcgat cgacatcctg
    22741 ccatgaagca attttctgct cctgtagaaa atgcaagtac tgttccaaat cacgttcata
    22801 gctttgtcgt gtattggcgg acaatccccg ttcaatattc aagtagtgga gataatcgtt
    22861 gatttgctct ttcataataa tccccctcat gcctctctat catatcagat accgcttcat
    22921 tctgatagtc tttttccata atctgagcaa agaaaaagcc gtctagacac tcagacggac
    22981 tggtttcttt tttcggctct ataatttatg ggactgatga atcagaattg attcttcagc
    23041 cccattttca ttttaggtat taaa
//
LOCUS       DAYYFU010000039        22395 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000039 DAYYFU010000000
VERSION     DAYYFU010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.039"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..649
                     /locus_tag="ACHA34_001792"
     CDS             <1..649
                     /locus_tag="ACHA34_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792410.1"
                     /translation="DTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLS
                     AVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMR
                     YQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITM
                     IHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            708..1148
                     /locus_tag="ACHA34_001793"
     CDS             708..1148
                     /locus_tag="ACHA34_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792411.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1160..1828
                     /locus_tag="ACHA34_001794"
     CDS             1160..1828
                     /locus_tag="ACHA34_001794"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8792412.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1892..2515
                     /locus_tag="ACHA34_001795"
     CDS             1892..2515
                     /locus_tag="ACHA34_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFM8792413.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2571..2957
                     /locus_tag="ACHA34_001796"
     CDS             2571..2957
                     /locus_tag="ACHA34_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFM8792414.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3170..4561
                     /locus_tag="ACHA34_001797"
     CDS             3170..4561
                     /locus_tag="ACHA34_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFM8792415.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5172..5540
                     /locus_tag="ACHA34_001798"
     CDS             5172..5540
                     /locus_tag="ACHA34_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFM8792416.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5720..6661
                     /locus_tag="ACHA34_001799"
     CDS             5720..6661
                     /locus_tag="ACHA34_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFM8792417.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6730..7461
                     /locus_tag="ACHA34_001800"
     CDS             6730..7461
                     /locus_tag="ACHA34_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8792418.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7605..9785
                     /locus_tag="ACHA34_001801"
     CDS             7605..9785
                     /locus_tag="ACHA34_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFM8792419.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9805..10287
                     /locus_tag="ACHA34_001802"
     CDS             9805..10287
                     /locus_tag="ACHA34_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFM8792420.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10303..11064
                     /locus_tag="ACHA34_001803"
     CDS             10303..11064
                     /locus_tag="ACHA34_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8792421.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11195..12529
                     /gene="murC"
                     /locus_tag="ACHA34_001804"
     CDS             11195..12529
                     /gene="murC"
                     /locus_tag="ACHA34_001804"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFM8792422.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12836..13321
                     /locus_tag="ACHA34_001805"
     CDS             12836..13321
                     /locus_tag="ACHA34_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFM8792423.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13314..13997
                     /locus_tag="ACHA34_001806"
     CDS             13314..13997
                     /locus_tag="ACHA34_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFM8792424.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14123..16768
                     /gene="polA"
                     /locus_tag="ACHA34_001807"
     CDS             14123..16768
                     /gene="polA"
                     /locus_tag="ACHA34_001807"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFM8792425.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16813..17649
                     /gene="mutM"
                     /locus_tag="ACHA34_001808"
     CDS             16813..17649
                     /gene="mutM"
                     /locus_tag="ACHA34_001808"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFM8792426.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17613..18242
                     /gene="coaE"
                     /locus_tag="ACHA34_001809"
     CDS             17613..18242
                     /gene="coaE"
                     /locus_tag="ACHA34_001809"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFM8792427.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            18294..18626
                     /locus_tag="ACHA34_001810"
     CDS             18294..18626
                     /locus_tag="ACHA34_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8792428.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18938..19429
                     /gene="nrdR"
                     /locus_tag="ACHA34_001811"
     CDS             18938..19429
                     /gene="nrdR"
                     /locus_tag="ACHA34_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFM8792429.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19452..20825
                     /locus_tag="ACHA34_001812"
     CDS             19452..20825
                     /locus_tag="ACHA34_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8792430.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20825..21751
                     /gene="dnaI"
                     /locus_tag="ACHA34_001813"
     CDS             20825..21751
                     /gene="dnaI"
                     /locus_tag="ACHA34_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFM8792431.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21880..22320
                     /locus_tag="ACHA34_001814"
     CDS             21880..22320
                     /locus_tag="ACHA34_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFM8792432.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         7588 a         3872 c         4885 g         6050 t
ORIGIN      
        1 agatactctg attgagcgat ctacatttct atatgttcaa ttgcaggaaa ataaaacatt
       61 gctgcatttt tcaccagagt tccatctaga ggggctaaca ttaggagaaa tctatcaagc
      121 agaagggctg tcggctgttt tgcacttttt taaggtagaa ttggaattac cagtaaaaga
      181 ttatatcgag ctttctcttg aatcttggga tcgagcagtg gttgaactgt ttccagaagg
      241 tttaatgatc gatacaaacg atgggaagat ccatctgact gcagcagaac tgcaaaaaca
      301 aatgcgttat caaatagatg acgaaaattc tttttctatt tttagacgtc agcaaaagat
      361 tctaaaaagc tttttaaaag tgatcagtaa aaaacggaat ctgatcaaaa cgacacagct
      421 gttgaaaaaa tatccaggtc tgcttcacac gtctattcaa tttcctcaat tgattacgat
      481 gatccatcgt tttatacgta tgcaacagct accatctaag aagctgtttc ttccattgac
      541 ggacacattt cgagtagtca acagagaaga taaaaagaaa gtgattgtga ttgactttac
      601 cagaaatcgg aatgtccttc accaagttgc aatggaaaag gccaattaag gctttttctt
      661 tttggatttt tttgtcataa taaatattag atgatggagg agataatatg atgcaaacat
      721 ggttagaaag gctaatacga tttcagcaga actatcacaa acttatgcag ggaagatatg
      781 cgaaatttga tcagttgaat cgcggtctat tgatcgctgc gcttgtcttt agtttgctaa
      841 gtcgttggtt gccctataga atcggacaat tgttgttcat cgtctttttc ggatggatga
      901 tatttcgttt cctatcaaaa aaaatctacc ccagactaaa tgagaatcaa cgttatttga
      961 aatatcttga tagagtcaaa gaaaaatatc atcagataaa aaacaaaaag caagtcaaaa
     1021 tggatcgaaa aagttatact ttttttgagt gcccgaattg tcatcaaaag cagcgtgcac
     1081 ctaaaggaaa aggacgaata cgtgtgacat gcaaaacatg tggtataaaa ttcgaaacga
     1141 atgtttaagg gagaaaagaa tgaaattagt attattacgt catggcgaaa gcgaagcaaa
     1201 ttttgaaaat tactggacag gatggctaga tgtcgcctta acagaaaaag gaaaagaaca
     1261 agcagcagtt gccggtcaaa aaatgcttga agccggactt caattcgatg ccgtttatac
     1321 ctctgttttg aaacgtgcga ttcttaccgg acaagtcgca ctagaagaaa tgggtcagtt
     1381 atggcttcct ataataaaaa catggcgtct aaatgaacgg cattatgggg atcttgtggg
     1441 taaaaacaaa gataagatga aagaaatata cggaaaagat caagtaaaaa catggcgcag
     1501 aggatatacg gaagttccgc cgttaaccgc agaaaataga tttgaccgca gatatgatca
     1561 cttggatccg catttgatcc catacggaga aagtctggaa atgacagtga atcggatcat
     1621 tcccttatgg caagatcatc tagcaccaaa actaaaagat cggcaagatt tactagttgt
     1681 gggtcatggt aatagcatcc gagcactgac aaaatattta gaagatgttc ctgaagatca
     1741 aatggatacg atcgatatcc caaatgcaca accaatccag tatacattag cagatgattt
     1801 gacaattctg gacaaaaaaa tactttaaag aacaccttta tctaaaagtg agagatagaa
     1861 aaaagaagca aaggaatgga acaaaaggaa catgaaaaat tatatatggg actttgatgg
     1921 tacattgttt gatacgtatc ccgcaatggt agatggtgca tggcaagcac taaaagattt
     1981 tggtatcagt atggacaaaa aagaaatcta cttcaaaatg aaaaagtatt ctactagtta
     2041 tttgatcaat gaatcaaact tgaatgccag agagtttaat gaattatttc accgatatga
     2101 aaaagaaagt acggaagttt cccgtccatt tccagaaacg aaacaagtac tggaaatgct
     2161 aaaagacaac ggcggtcgtc atttcatcct gacccaccgt ttgacagaat caacatgggg
     2221 cctgttaaaa gaacatcgat tggcacatct gattgaggaa gtagtaggga tcgatcagga
     2281 ctttcctcgt aaacctgatc cagcatcttt aaattatttg atcgatactt ttcatttaga
     2341 aagaacagac acgatgatga tcggcgatcg tcgtttagat atcgaagcag ggaaaaatgc
     2401 aggagtcgct acttgtcttt acgacatcga tcattttctg ggtgagatcc cggcagatta
     2461 tgtggtagga aatctgaatg aaatccttac cttgcagagc atcattaaaa agtagtatcc
     2521 cctttcaaag gagatatgtt ataattttca taaaggagtg ataaaattat atggaaatca
     2581 gaagacaaaa agaagttgta gaagcatttc attatgatat gagattgccg aaccaagaag
     2641 tagaaacaga tttgaaagta ggcttctcgc ctttaaaaga aacgatcgaa aactatccga
     2701 aagaacatag catcgtagga gcacgcttag agttccgttt agtgtttgat acatatgttt
     2761 tatctggaag tgtgagtcag atcaatcata tcctcaatcg gaaaattgaa ggtcaaaatg
     2821 atgtcacaca agaagaagtg gacgagctag tagcaccgtt attcgatatc gtacgccgaa
     2881 tgtcttatga ggtgacagag atcgccttag atcagcctgg ggtaaaattg aattttcaat
     2941 cagaaaaaca agaatagaca gtaaaaacat acctgcgata gattaaagct atcacaggta
     3001 tgttttttta ttactaattg taagcaaaaa gaatgtcatt tcgataaaaa tcgtctaaaa
     3061 tataaaagtc actgaaaaaa attttttcag gaggatggtt gaagaatcat tctcgggaac
     3121 ctttacttcc ttcgatggac ttgtcattaa taatatgagg aggagaaaaa tgacaacact
     3181 catcacagtt ttagtggttc tagtttttgc ggcattatta tatgtgttct atcgtatgca
     3241 aaagcgccat acaaaatttt caacacgcgt ctttacagca ttaggagttg gtatcgtctt
     3301 tggcggtctg cttcaattga tctttggtgt agacgacaaa gtcgttactc aatcaatgga
     3361 atggatcggg attgtaggtc aaggatatgt atcattcttg caaatgctag ttatgccttt
     3421 agtgtttatt tcgatcattg gtgcattcac aaaaatgaaa gtttctgaaa agctagggaa
     3481 aattagtgcg actgtattga cttctttact tggaacaaca gctattgcag cattgatcgg
     3541 aattggttca acaatgctgt tcggactcca aggagctaaa ttcacacaag gagctgcaga
     3601 aacagcacga atctctgaac tggcaacacg tcaggaaaca gttgaaaact tatcgattcc
     3661 acagcagatt ttatccttta tccctaaaaa cgtttttgct gatttagcag gtagtcgtgc
     3721 aacaagtacg attggagtcg ttattttcgc ggcatttgtc ggaatcgctt atctaggcgt
     3781 gaaaagaaaa gcgccaaaag aagcagcatt cttttcagat ttagtcgaca gtctgtataa
     3841 aatcattatg cggatcgtga cgcttgtttt gcgtttgaca ccttatggag tatttgcttt
     3901 gatgacaaac gttttggcaa caagtgattt tgcagctatc gtcaatttag gtaaatttgt
     3961 cgttgcttct tacgtagcat tgcttgctgt tttgattgtc cactcactga ttttgattgg
     4021 tgcaaaagtc aatccaatca actatttcaa aaaagccttt ccggtattga gctttgcgtt
     4081 tacttcacgt tcaagtgctg gagctttgcc attgaatatc gagactcaga caaaagcttt
     4141 aggagtagat gatgcaacag ctaatttctc agcaagtttt ggtttgtcta ttggacaaaa
     4201 cggctgtgct ggcgtttatc cagctatgtt ggcaacgatc gtcgcaccag cagctggagt
     4261 gaatgtcttt agtttggaat ttattttgat gcttgtggca attgtaacaa tcagttcttt
     4321 tggtgtagct ggagtcggtg ggggcgcaac atttgcttca ctgatcgttt taggggcaat
     4381 gaacttgcca gtagcaatcg ttgggttagt tatctctgtt gaaccattaa tcgatatggc
     4441 acgtacagca gtaaacgtca gcggtagcat ggtagctggt atcgtgacaa gtgcacgcat
     4501 caaagattta gatcgcgatg tcatcaatga tgattctaaa gtgatcgatg tgaatgcata
     4561 gtcaaaaaga ggttgtgata gaagtggtca gctttaagaa ataagaagaa gtttcctaaa
     4621 aatagtttct caaatttttg tgaaatttcg gcttatttcc gagaagctac ttctgaaaca
     4681 ccgtgaataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     4741 aaatagttcc tcatatttta cgtttggttg agcttattac cgcagatgca agcttttgaa
     4801 caccgtttaa gaaagaggtc gtgagcctgc cgtttcactc tgagtcacaa agagcaattt
     4861 caaaaaatag cttctcatgt ttgttgaata ttgtgtcttg tgaccgagga gttgtcagga
     4921 gaacaccgtt taccagaggt tgtgacttgt ttttagtcac gacctctttt tttatttatt
     4981 ccatataaat acacattatt tattccgaaa agtgaaagcg caaataacaa aaacaaccaa
     5041 cttatttatt attaaagaaa agcgtattta agtaggacga ttctgttgag agctttcgta
     5101 gattttccca ttttcctatg gtaaactgac aaagttgaga atgttagaac agatcgttta
     5161 ggaggaaaca gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa
     5221 aaaatgcaac gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg
     5281 ggacgcttat aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc
     5341 gagcatttgt tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt
     5401 acagtaaaga tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg
     5461 atatggtaca gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact
     5521 ttgcggttac aggtaaataa tagaaataaa gactttgttt ttctgtgcga aacaggaaag
     5581 cgaagtcttt ttaattcaaa taataatatt atgtaaacaa aaaactttcc atgcgtaatt
     5641 atacacaagc aaataaatcg tcaaatgcga acgccttctt tatactgttt aggaacgaaa
     5701 agaaggaagg aagaaataca tggattatca agtactgctt tattacaatt atacaaaaat
     5761 cgaagaacca gaggaatttg ccaaaaatca tttagccttt tgcaaatctt tggatcttaa
     5821 aggtcgtatt ctagtagctg aagaaggaat aaatggtact gtgtctggaa ccgttgaaaa
     5881 cacgactgcc tatatgaaag caatgaaagc tgatcctcga tttgctgata cgatgttcaa
     5941 gatcgatcca agcgaaggac atgcctttaa gaagatgttt gtaagatacc gtccagagct
     6001 tgtaagttta aagctagaag aggacattga tccaaaccaa gtgacaggtg cctatctttc
     6061 accgcaagaa tttaaagaag ctattttaga tgaacagaca gtggtgattg atgcacggaa
     6121 tgattatgaa tatgacttgg gacattttcg aggggctgta cgcccagata tacgcagttt
     6181 tcgagagctt ccagactgga tccgcgagca caaagaagaa tttatggaaa aacgggtagt
     6241 gacctactgt actggtggca ttcgttgcga gaagttttct ggttggcttg tccgtgaagg
     6301 attcaaagat gtcggacagc tgcatggcgg tattgctact tatggtaagg atcctgaagt
     6361 ccaaggagaa ttatgggacg gcaagatgta tgtgtttgat gaacggatcg ctgtcgatat
     6421 caaccaagta gataaacaaa tcgtcggtcg cgactggttt gacggaacac catgtgaacg
     6481 atatatcaat tgtagcaatc ctgagtgcaa tcgtcagatt cttgcttcca ttgaaaatga
     6541 agaaaaacat ttaggagcat gtagcataga gtgtgcgaaa catccgcgca atcgctatgt
     6601 tcaagaccat ggaatctctc cagaagaagc agaactgcgg tttacagctt taattaaata
     6661 gaaaacaata gttttgattg tcgaccattt ccaatatgcg ctatactagc gttgaggatg
     6721 gtgatccaca tgaaatcatt gatttatgct attggggatg tccatggcaa acatgagctt
     6781 tttgaaaaac ttattcacta ttatgatcca aagatccatc agcttgtttt attaggggat
     6841 ctcaatgatc gaggtcctcg tacaaaagaa tgttttttat taggaaagca attagtcgaa
     6901 gaaacaggag ccatttacct ccggggaaac catgaagaat attttttgca atttctgcaa
     6961 tcaccggagg actggtatgc gccttatatg agaaatggtg gaaaagaaac gtttgaaagc
     7021 ctgattcacc caggggcatc ggaggaatac tccccaactg aaatctcgat gatgatccaa
     7081 tcgagatatt cagaactgat taaattttta gtccagcgcc ccttgtatta tgaatgggaa
     7141 aattatcttt ttgtccatgc cggtgtggat ttgacaaaga aagactggcg taagacaagt
     7201 ctaagagact ttatttggat aagggaacca tttcatgaag ggaaaaacaa tacaggtaag
     7261 acaattgtat ttggacatac gattacacca atgctccatg gagatatgca gacaacagac
     7321 ctctggattc aggatcataa aatcggaatg gacggtgggg ctgtattcgg agggtcattg
     7381 catggggttg tttttgacaa ggaaggtatt cttcaagata ttgaatatca gaacaacgaa
     7441 ggaccttggc atggaatctg actaatggaa agaatgaagg agctgtgact ttgttgcggc
     7501 tctttttatt tttatagaga atattgtttc tttcagtagt ttaacccagg ataaaaagta
     7561 tgatagaata aagaagcaat ttttagcaga aaagaggcat tttaatgacg gaaaatttga
     7621 accagacgat cattcagctt gtacaaaaag aactttcaga ctatcgtcct aagcagttga
     7681 ctactgtact taatttgtta aatgaaggaa acactgttcc ttttatcgcg cgttaccgaa
     7741 aagaaatgac tggaagtctt gatgaagtcc agattcgtga aatcgaagag cggtatgcct
     7801 atcttgaaaa cttagaaaaa cgtaaaactg aagtgattcg gctaatcgat gaacaaggga
     7861 aactgactcc cgagttagaa gcagaaatca cccaagccgt gaaaatgcaa caagtggaag
     7921 atttgtatcg tccatacaaa caaaaacgac ggacaaaagc aacgattgct aaagaaaaag
     7981 gacttgaacc actcgcgaag tggctgatgc agcttacaga tggagaggtg caatctgaag
     8041 cagaaaaata tattgacaaa gaaaaagaag tctcttctgc cgaagaagcg ttgcacggtg
     8101 ctcatgagat tattgctgaa caagtcagcg ataatgcaaa atttcggact tggattcggt
     8161 cttatacgta taacaaaggt atgtatgtca gcaatgtcaa ggatgagcaa gcagatgaaa
     8221 aaggcgtata tgaaatgtat tatgactttg cagaacccgt ccataaaatg gtttctcatc
     8281 gaatattagc tactaatcgt ggagaaaaag aagatattct gaaagtattc tttcaagtag
     8341 atgaagcagc aattttagct tacttggatc gccaaatagt caaaaatcct gcctctcctt
     8401 cttcttcatt cgtcagagaa gcctatcagg atagttataa acgctttatt cagccagcaa
     8461 tcgagcgaga actgcgcaat gaattgactg aaaaggcaga tgagcaagca atcgctattt
     8521 tcggcgaaaa cctccgtaat cttttgttgc aaccgccact aaaaggtaaa gttgtcctgg
     8581 gatttgatcc ggcttatcgt actggatgta aactagcagt cgtagatgcg acagggaaag
     8641 ttttagcaat cgaagttatt tatcctcata aacctgcagc gcaagcaaaa agagaagctg
     8701 ctggaccagc ttttatccaa ttaattaata aatatcaagt agatatggta gctatcggaa
     8761 atggtacagc cagtcgggaa tctgaacttt ttgtggctga gcagttgaaa tccgccgacc
     8821 ataaaaccta ttacgctatc gtcaacgaag caggcgcttc tgtttactcc gctagcgaaa
     8881 ttgcacgaaa agaatttccg catctacaag ttgaagaaag aagtgctgtt tctattgccc
     8941 gtcgcctgca agatccttta gctgaattag tcaaaatcga tcctaaagct gtcggagtag
     9001 gccagtacca acatgacgtt tctcaaaaac gtttggctga acaattggat tttgttgtgg
     9061 aaacagcggt taaccaagta ggtgtggatg tgaatacagc aagcccgcag ctcttgcagc
     9121 atatatcagg attgaataaa acaactgccc aaaacattgt catttatcgt gaagaaaacg
     9181 gagaattcac cgcacgaact caattgaaaa aagtgccacg cttaggacca aaagcatatg
     9241 aacaagcaat cggattcttg cgagtccctg gtgggaaaaa cattttggac aacacaggta
     9301 tccatcccga aagttattct attgcaaaag aaatattaac ttcagtccaa ctttcagaaa
     9361 aagagcttgg tacagaagaa gcagtagaaa aattgacacg attatccgta gagaaactgg
     9421 ctgaaagcct atcagtcgga gaagaaacgt tagccgatat tttagctggg ctgacacaac
     9481 ctggacgaga tatgcgtgat gagatgccag cgcccttatt acgtacagat gtattgagta
     9541 tggaagactt gaaaccaggg atggaattga ctggaacggt ccgtaatgtc attgacttcg
     9601 gcgcatttgt tgatatcggt gtgaagcaag acggacttgt ccatatatcg aaactcagca
     9661 aaaaattcgt caagcatcct acagatgtcg tgtcagtagg agatatcgta acagtttgga
     9721 tagaacaagt cgatacgaaa aaaggcagaa tcagtttgac gatgctttcc ccttatgaag
     9781 agtaacaatg aggagtaaca aaaattgaca caagaagaac tgcagcagtt agtagaagag
     9841 atatcattaa aaagcttcca gcttcctttc aagcaccaag caatgttcaa tccacgctta
     9901 cgtacaactg gcgggcgcta tcatttaaac gatcatcatt tagattttaa tgcgaagcta
     9961 tttgaagagt cagacgaaga gacgattgca ggaatcatca aacatgaact ttgccattat
    10021 catcttcatc tagctggaag agggtatcag cataaagata tggatttcaa gtatcttttg
    10081 aaaaaaacag gagggttgcg ctatgcacca gccttgacga agaaacagat aaagatggag
    10141 tattatcgat gccaagattg ttctgccacg atctgccgca agagaagaat cgatgtacgc
    10201 aaatatcgtt gtggaagatg tcatggagaa atcaaatgga ttgagacaaa aagagaagac
    10261 tttaatgaag cacaaaaaat cagatgaaag gatagaaggc ctttggaaaa aagtgagcag
    10321 aggaatctaa aagactggaa tcagctatta aattttttag ccgcacctga agagggaaat
    10381 tttgaggaac tgacggcacc tacacttatt ttagctggaa actgtcttcc tatcttagct
    10441 gataaagcag cacaaatgta tttaaatggc caagtagatc aattgttttt ggtcggaggg
    10501 gtggggcatg cgactagaat cctttatgag aattttgaaa agcaaggatt tcattttgaa
    10561 gatggcatga gtgaaagtga aatctgtcgt cagtatctaa aagaagtcta tgatttgccc
    10621 gataaggctt ttctgataga atcgaagtct acaaattctg gagagaacgc cactttttca
    10681 ctggagattc ttcattcctt aggagctgta ccagaaaaaa tcttattgat gaatgatccg
    10741 acactgcaac gcaggactcg agcaactttt gagaaagtat ggcaaaatga acaaactatt
    10801 ttcgcgaatg ctgtgccatt tgtgccggaa atccttcact tttctgaaga gatcatcttt
    10861 acagcaaaag aactgaatca tcaatggcct aaggaatatt tccatgcgct agtattagga
    10921 gaaatggaac gtctccatga cgatgagaac ggctatggtc cgaaaggaaa agattttatt
    10981 cctcacattg atatatcaga ggaagtttgg tcttcttaca ccagaattaa agagtcaata
    11041 aaaacggatt tttctcgtac ttgaccattt tagcaagcga atcctccttt ccaaataaat
    11101 ttggttgaaa ttttcaatcg tttataagat aatgaggagc gtgttgaatg taatagctaa
    11161 aagaatggac gctagataag agaagaggat tcaaatggaa aatcaaaata aattgtatca
    11221 ttttgttggt atcaaaggtt caggaatgag ctccttggct cttgttttac atgaacaagg
    11281 tctaaatgtc caaggatcag acattgaaaa atatttcttt acacaaagag atttagaaaa
    11341 agcaaatatc acgattctgc catttaacgc agataatgta aaaccaggga tgacgatcat
    11401 tgcaggaaat gcatttcctg attcacatga agaaatccag cgagcaaaag aattaggttt
    11461 ggaagtgatt cgctatcacg actttattgg tcattttatc caaaattata cgagtatcgc
    11521 tgtaacaggc tctcacggaa aaacaagtac gactggactg ctctctcatg tattgagtgg
    11581 tgtccgtcca acaagttatc taattggaga tggaacagga catggtgatc cgcaagcgga
    11641 attcttttca tttgaggcct gtgaatatcg ccgtcatttc cttgcctatt caccagacta
    11701 tgcaatcatg accaatatcg attttgatca tccggattat tatacaagta tcgacgatgt
    11761 atttacagct ttccaaacaa tggctggaca agtgaaaaaa gctatttttg cgtatggtga
    11821 cgatgcttat ttacgcaaat taaaagcaaa tgtgccgatt tattattatg gtgtcacaga
    11881 aaacgatgat atccaagcac gaaacatcga acgcacaact tctggttctg cttttgatgt
    11941 ctatcatggg gatgaatttg ttgggcactt cactgtacca gcattcggta aacacaatat
    12001 cttaaatgct ttaggtgtta ttgcagtagc ttatttcgaa aaactggatc tgaaagaagt
    12061 tgcagaagaa atgctgactt tcccaggagt aaaacgtcga ttcagtgaaa agatcgtagc
    12121 agacatgacc gtagtcgatg attatgcgca tcaccctgca gagatcaaag caacgattga
    12181 tggcgcacgc caaaaatatc cagataaaga aataattgca gttttccaac cccatacgtt
    12241 tacgcgtacg attgcattga tggatgaatt tgcagaagca cttgatttag ctgataaagt
    12301 ttacttatgc gatatttttg gttcggctcg ggaagaacaa ggaaacgtta agattgaaga
    12361 ccttggagcg aaaatcaaaa aaggtggaga agtcatcaaa gaaaataatg tatctccatt
    12421 gttagattat catgatgcag tagtaatctt catgggtgct ggagatgttc aaaaatttga
    12481 acaagcgtat gaaaaacttt taagcagcac gacgaaaaat gtactgtaat acaattactg
    12541 cagtgtcctt ataaaaatag aaagaggggg agtggcaaaa atcttgccaa gccccctttt
    12601 ttgcaactta tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaatcaaa
    12661 tcaaaaacat gagaagctgt tttttcttgg ctgttcttat tatttgatgc aggacagctt
    12721 tttcacgacc tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt
    12781 acgctacttt tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa
    12841 cataggtaag ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt
    12901 atccttaaca gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga
    12961 tgcaaatcct ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat
    13021 cgttccatca gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg
    13081 accaggatca catgttgtca atttttctgt caattttatt gaaccggttt tccattatga
    13141 aacactgact tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat
    13201 ttctgtagaa gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca
    13261 accgccacaa gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata
    13321 atccaagtac atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag
    13381 gtatgggact tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc
    13441 aattagcaac ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac
    13501 tgattttagt aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg
    13561 gatttattgc ctactctctt ttgaatggca taatattagc ggttactctt gcatactacg
    13621 atattggaac agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg
    13681 ttgtcggagt aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt
    13741 taatcggagt gatcattgca ctagtcttga acatcttcat tttgagaagt agcgtagttg
    13801 agctgttcat ctccctactg ttagtcgtca tctttgcagg gattacagct tatgataacc
    13861 aaagcatccg taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat
    13921 tcatggcatt gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct
    13981 ttggacggaa caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg
    14041 gtatggtccc gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac
    14101 tttacagtaa ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag
    14161 tgtggctttt cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa
    14221 cggtcttcat acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa
    14281 agagcaaccg actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga
    14341 aatgtatgcg gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat
    14401 gccatatata cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta
    14461 tgaagcagat gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt
    14521 cgtcgtgctt tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga
    14581 tatcactgta aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa
    14641 atatgatggg ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc
    14701 tgacaatatc cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca
    14761 atatggctca gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa
    14821 agaaaatctg ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa
    14881 tacttcagcg cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga
    14941 taaactcgtt ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat
    15001 cgtagaagaa gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga
    15061 ggaaatgttt actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac
    15121 agaggaaatc gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct
    15181 atctgtattc gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa
    15241 agtatatgat gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg
    15301 aatcgatttt gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga
    15361 tatcgaagga atagctgctc attacggata caacgatatc caatcagatg aagctgtata
    15421 cggcaaaggg gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc
    15481 gcgaaaagta gctgcaatca atgcgttgac accaaaattg agccaagaac tggcagaaaa
    15541 aaaccaagaa gatcttttcc ggaaaatgga actgccattg tctatcattt tggcagagat
    15601 ggaaataagc gggatcaaag tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc
    15661 tgctcgctta agagagatcg aacaaaaaat ctacgaagaa gccggcgaag aattcaattt
    15721 gaattcacca aagcaattag gagtgatctt atttgaaaaa atgggattac ccgttatcaa
    15781 aaaaacaaaa actggatatt ccacagccgt agatgtactg gaacagcttc gtgaacaagc
    15841 accaatcgta gaggatatcc tcacctatcg tcaaattgca aaaattcaat ctacctatgt
    15901 cgagggactg ctgaaagtga tccaatcaga tggaaaagtg catacccgtt atgtccagac
    15961 attgacccaa acaggacgat taagctctgt tgatcctaat ttgcagaaca tcccgattcg
    16021 attagatgaa gggagaaaga tacgtcaggc atttgttcct agagaaaaag actggctgat
    16081 ttattcttct gactattcac aaatcgagtt acgcgtactt gcgcatatct caaatgatga
    16141 acatttgaaa gctgccttcc tagaaggtca agatatccat gccagcacag cgatgcgtgt
    16201 atttggaatc gaaaaggcag aggatgtcac accaaatatg cgtaggcagg caaaagcagt
    16261 gaattttggt atcgtgtatg gcatctctga ttacggactt tcacaaaatc taggaatcag
    16321 ccgaaaagca gcacagcaat acatcgatac gtactttgaa aaatacccag gtgtcaaaga
    16381 atatatggaa agaatcgtac gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca
    16441 ccgccgtcga tatctgccag acatcaattc aagaaattac aatatccgtt cttttgcgga
    16501 acgaacagct atcaatacac caatccaagg aagtgctgct gatattttga aaatcgcgat
    16561 gatcgaattg gataaacgat taaaagaaac aggtcttcaa gctacgatgc tgttacaagt
    16621 tcatgatgaa cttgtcttcg aggtaccgga aaaagaactt gaatcattag ataaattggt
    16681 gaaggaagtt atggaacaag ctgtttctct tcatgtacca ctgatcactg acagcagttg
    16741 gggaaaaacg tggtacgaag caaaataaac attgtttaag acagagcaat ataatgaagg
    16801 tgaaaagaaa aaatgccaga attaccagaa gtagaaaccg ttcgtaaagg attggaacgg
    16861 ttagtagtag gaaaaacgat tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa
    16921 ccagagacac ccatctttga ggcttctctt gttggcgaaa ccatccaatc cattggacgt
    16981 agagggaagt ttttaatttt tcatctcgat cattgtgaac tgatctcaca cttgcgaatg
    17041 gaaggaaaat atcaatttac gaaagaaaat acaccaatcg ataaacatac gcatgtactt
    17101 ttcttttttg aggatggaag ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg
    17161 acaatcgtcg aaaaaggagc aagtgcaact taccgaggaa tcatgaaatt gggacctgag
    17221 cctttgccag atagcttctt attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa
    17281 gcaatcaagc cattgctgct agaccaacgt ctagtgactg gtctaggtaa tatttatgta
    17341 gatgaagcat tatgggaagc gaagatccat ccagaacaac cggcgaacac actgcgcccc
    17401 aaagaggtag aacaattacg tctatcgatc attgatgttt tggatcgtgc aatagaagcc
    17461 ggaggtacca cgattcgcag ttatttgaat gcattaggag aatcaggcgg atttcaagta
    17521 gcactgcatg tctatcagca aactggcaaa ccatgtatac gttgtggaac gccgatcgtg
    17581 aaaacaaaag tggcacaaag ggggacgcac tattgtccga attgtcaaag gctaaaaatc
    17641 gcaaagtagg atttgtttta ggtttgacgg gtggaatagc taccggtaaa agtacggcag
    17701 ccaaagtctt tcagtctcat ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa
    17761 ctgttgagcc tggaacgctt gctctgagga aaattgtatc agtgtttggc caagaggtat
    17821 tgcagcctga tgggcaacta gatcgaggga aacttgggat gattgttttt ccatcaaagg
    17881 aaaaacggca aaaacttgat cagctattag atccgtttat ccgaaaagcg ataaaagatc
    17941 aaatcgcctc tctaagttcc aatcatcctt tagtaattgt agatatccca ttgctctacg
    18001 aaggtcatta tgaccactat atggatgcag tagctgtcgt ttatactacg ccagaaactc
    18061 agcttcagcg tttgatgaaa cgtaatcaac tgacgctaga acaagcgcag cagagaatta
    18121 gtagtcaact tccaatcgaa gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg
    18181 gaacaaaaga aatacttgtg ggacaaatcg aaaattggct ttttgctaat cgctttcttt
    18241 gagtatctgt gtcaattttg ctatcctgag tatatagaaa gtgagggata gcgatgggat
    18301 cattaaaaaa tcaaaaggat aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc
    18361 tcaataaaga aaatctaatc gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag
    18421 aaaagcagta ccaagcaaga atcgatgaaa tagaagacca gcaggcagaa gaagaagcga
    18481 agcggcaaga tttgactcaa gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc
    18541 tcccagaaag tgaccacatt cccgatgagc aaaaaatcga ggaacagcga atgaaacact
    18601 atacaggaat cgaagagaag ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga
    18661 tgagaaggtg ttcatctgct tcaagttttt tgtgctagat gactaaatca gcttttgtgt
    18721 aagttatttc tcagtaatca aggtgatcag tattggaaaa ttgaaattta gtaaggacag
    18781 gggacacaat tacgcatgaa agtagaaaaa attgttctta tatccgtgtt ttttgggtag
    18841 gacagtcgcg tttgtgattt gtttcgtgtt ataatacaac tagttgtaaa gaaagacaca
    18901 tcctttatat aaaatgcgat gcaagaggtg agacaaaatg agatgtccca gatgtcaaca
    18961 taataattca cgagtaatcg acagccgtca agcagatgat ggacgtgcga tccgtcgccg
    19021 tcgcgaatgt gagaattgtt cgttccgatt cactacattt gaacgaattg aagctgctcc
    19081 gttattagta atcaagaaaa acggggaacg cgaagagttc aatcgcgata aaattttaag
    19141 aggactcatc cgttcagcag aaaaaagacc tgtagctatg gaacaaatgg aacaaatcgt
    19201 caaccatgtc gaaaaccgcg tacgcagtct cggagaaaac gaagtatcga cgaatctaat
    19261 tggtgaatat gtgatggaag atttagttga tttagacgaa atcgcttata ttcgttttgc
    19321 aagtgtttat cgccagttca aagatatgtc tgttttctta aaagagttgc aagatatcgt
    19381 ggataaagca aaagaaaata ataaagagaa taataaagaa aacaattaag aaacaaaaag
    19441 gaggcatttc cttggaaaat gcttggaaag agcttcaacc aaaaaatatt taccaaggaa
    19501 gaaaagcggc tccgctgaga gaagacagca agaacgcctt gctgtatctt tatcagccac
    19561 taatcggcgg agaagccttg agtatctatt tcaccctgtt atcagaaatc tctcaagaat
    19621 ctggcaaagg tcctgaaggg atgcatgcag atctgcttag tagtctaggc tgcggtatcc
    19681 cgcaatttta tgatgcaaga aaaaagctag aaggaatcgg cttactggat gtttatttta
    19741 aagaagatag tgatctggga cctcgtttta tttatgagct gttagaacca ctcattccta
    19801 aagcattttt caaagactct gttctggctt ttcttcttct ggaaaaagtt ggtgaacgac
    19861 gattccagaa gcttacccaa ttctttgaac ctaaaaggca tacagcagag ggatatcaaa
    19921 aaatcacaaa aaaattttta gatgtttatc gcttcaatga agcagtgtac gctgctaatc
    19981 aagaaaacat cgaatcgctg aataaatctt ttgaagaatc tgaatcagta gcattacttg
    20041 aagcagattc gacattcgat tggcagtttt tgaatgattg gctggcaaaa aaacatattg
    20101 caagtcttga tgatccaatc atcaaacaag tcgacatgta tcatcagctt tacggtttcg
    20161 atgaattgac acttggcgaa ctgatcagtc aatcttatga ctttagtgaa caaaaagtat
    20221 ctgaaaaaga actgcaaaaa gccattttgg caatgcagag acgtccggtg aagcaaacaa
    20281 aagaaacagt tgcggtttct gaaaacagta atgaaccctc gtcaaacgaa catttgacaa
    20341 acgcagcgct tcagctgata aaagaagcac agagcattcc accgatgaaa tatctggaag
    20401 caatcaagtc agaaaaaggc ggatatgttt ctaaacaaga aaactggtta ctgcaagatc
    20461 ttgtttctca atcaggtctt tccagtagcg tgatcaatgt tttgctgaat tatgtactag
    20521 tgatcaagaa taatgctagc ttaaatgcca gttatgtaaa tacagtggca aatgaatggg
    20581 cacaaaagaa aatcgccaca gcagaagaag cgatccagca cattcggcaa ataagtaatc
    20641 aagcaaaaca atcaaaacaa aagaagcaaa caaattatca aactggcaag cgaaatgttc
    20701 gacgggaaaa gcttcctgat tgggtcaacc agccaaaaga tgaaaaacag atcagcagcg
    20761 agaaaaaagc agagattgac cgccgtttta aagaatacct ggcaaaaaag gagggtgata
    20821 tctagtggaa gacgtaggca aagaattgaa gaggatcatc agcaaacgca attatcaaga
    20881 aaagtatgct gaaatgatcg ctgaagtctt aaatgatgcg gatgttcaag cttttctaag
    20941 cgcacaccaa gaagaattga cacaagcgga tattgaaaaa agttacgcta aattgtatga
    21001 attcgtgcaa gaaaagcgta aatatcaaaa aaacgatcca acgatgatcg ctcccggcta
    21061 tgagccgaag ctgacgttga attttcatta tgtggatgtc acttatgtac caacggaagc
    21121 attgttagca cggcaaagac aagaagaaat cagaagccga gtaaaagcaa tggatatgcc
    21181 aaaagatatt caatcggctt cttttgaaaa ctttgaacgt acagatggac gaggatatgc
    21241 gagcttagaa gccctagatt ttgtagagaa atatactgct gatccaaaag ggtatcataa
    21301 aggactgtat ctagtaggaa gttttggtat tggaaaaaca tatttattag gagcaacagc
    21361 gagagaatta gcgattgctg gattcaccac gacgctagtc cattttccga catttgccgt
    21421 tgagatgaaa caggcaatcg gaaaagatca agtggcagaa aagttagatg cagtgaaacg
    21481 ttcacctatt ttgatgatcg atgacattgg tgcagatgcg atgagtagtt ggatacgaga
    21541 cgaagtattc ggtgttatcc ttcaatatcg gatgcaggaa cagttaccaa ccttcttctc
    21601 atcgaatttt acaatggatg aattggaaca gcatttgtcc gtcactcaac gtggagatga
    21661 agagccgcta aaagcgaaac ggataatgga acgcatacgt tatttgacaa aagaaatcga
    21721 aatgactggt cgcaaccgac gcaatggttg aacagttgac aagtccaact gacatttgac
    21781 ctagtagtaa aagcaggtca gcgaatcatt atatggacag aaatgaccgg agacaatcac
    21841 cgcaacaagt aatgcagaaa cttaaacgga ggatttttta tgagggctat tttttctagt
    21901 atatctgtag ttggtctatt ttttctttta gcaggctgcg gtagcgcaga tagcaacaca
    21961 ggttctgttg attcgattga tgcgggtcaa acagtaaaaa caagcagtac gaaaacggag
    22021 agacaaagca tatttgtcgg aacgattgaa agtatcactc aagtagaagc agaagaagaa
    22081 acgcttcagt tgaaactcgt cgatgtcatg gccaaagaag atccagcgaa tatgggaacg
    22141 tcattcagca atgacggagt gatcttaaat gctactgctg aaaaaataag cgtggacata
    22201 aatgaattga aaatcggtga caaagtccaa tttacattag ttgagatgcc gatcatgacg
    22261 atgtctatcc caccgcaagt tcctggagat tcgattattg atgtttcatt gaacaaataa
    22321 ccaataacat tttttcaaaa ataagaaaat gttgttttat agaacgaaaa ggtatatttt
    22381 ttaaggagat atggg
//
LOCUS       DAYYFU010000040        22031 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000040 DAYYFU010000000
VERSION     DAYYFU010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.041"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            367..555
                     /locus_tag="ACHA34_001815"
     CDS             367..555
                     /locus_tag="ACHA34_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792433.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="ACHA34_001816"
     CDS             545..805
                     /locus_tag="ACHA34_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6275 family protein"
                     /protein_id="HFM8792434.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            846..1073
                     /locus_tag="ACHA34_001817"
     CDS             846..1073
                     /locus_tag="ACHA34_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792435.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1138..1710
                     /locus_tag="ACHA34_001818"
     CDS             1138..1710
                     /locus_tag="ACHA34_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792436.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="ACHA34_001819"
     CDS             1711..2022
                     /locus_tag="ACHA34_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8792437.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="ACHA34_001820"
     CDS             2013..2273
                     /locus_tag="ACHA34_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792438.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="ACHA34_001821"
     CDS             2434..2793
                     /locus_tag="ACHA34_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792439.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="ACHA34_001822"
     CDS             2790..4433
                     /locus_tag="ACHA34_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8792440.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4468..5550
                     /locus_tag="ACHA34_001823"
     CDS             4468..5550
                     /locus_tag="ACHA34_001823"
                     /inference="COORDINATES: protein motif:HMM:NF028182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage abortive infection protein"
                     /protein_id="HFM8792441.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5594..6766
                     /locus_tag="ACHA34_001824"
     CDS             5594..6766
                     /locus_tag="ACHA34_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8792442.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6753..7457
                     /locus_tag="ACHA34_001825"
     CDS             6753..7457
                     /locus_tag="ACHA34_001825"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM8792443.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7462..8622
                     /locus_tag="ACHA34_001826"
     CDS             7462..8622
                     /locus_tag="ACHA34_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8792444.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8698..8976
                     /locus_tag="ACHA34_001827"
     CDS             8698..8976
                     /locus_tag="ACHA34_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HFM8792445.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8957..9328
                     /locus_tag="ACHA34_001828"
     CDS             8957..9328
                     /locus_tag="ACHA34_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HFM8792446.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            9344..9748
                     /locus_tag="ACHA34_001829"
     CDS             9344..9748
                     /locus_tag="ACHA34_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792447.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9745..10155
                     /locus_tag="ACHA34_001830"
     CDS             9745..10155
                     /locus_tag="ACHA34_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792448.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            10216..10803
                     /locus_tag="ACHA34_001831"
     CDS             10216..10803
                     /locus_tag="ACHA34_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8792449.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10876..11343
                     /locus_tag="ACHA34_001832"
     CDS             10876..11343
                     /locus_tag="ACHA34_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HFM8792450.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            11389..11697
                     /locus_tag="ACHA34_001833"
     CDS             11389..11697
                     /locus_tag="ACHA34_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792451.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11892..18626
                     /locus_tag="ACHA34_001834"
     CDS             11892..18626
                     /locus_tag="ACHA34_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8792452.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18630..19502
                     /locus_tag="ACHA34_001835"
     CDS             18630..19502
                     /locus_tag="ACHA34_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFM8792453.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            19499..20548
                     /locus_tag="ACHA34_001836"
     CDS             19499..20548
                     /locus_tag="ACHA34_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792454.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            20552..21376
                     /locus_tag="ACHA34_001837"
     CDS             20552..21376
                     /locus_tag="ACHA34_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792455.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21394..>22031
                     /locus_tag="ACHA34_001838"
     CDS             21394..>22031
                     /locus_tag="ACHA34_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM8792456.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         7878 a         3297 c         4671 g         6185 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tcaaaatgtt tcaccgaata
     5161 gttaaaacat taaatgagta ttatgacgat tataatgatt ttgatgtgaa gaggtataca
     5221 aaatatatag ggacattaag aacacaaatt tctccagctg aattccaagt gattttattc
     5281 aattctctat atataaaacg aggttttggc ttaggaattc aacttattgg aagtggtttt
     5341 tttggagatg attttgattt tgaaactaat caacattttg aaacaagtat caatgaacag
     5401 tggtttctat cactatcaac tgttgacagt gataactcta taaagcgaca aaaattatct
     5461 gaatatatta aagaattagg atccgtagaa tataaaaaaa tagctaattt tgaaagttta
     5521 tataaactct ttaatgaaac taagctctag aaatagggct tttttatttt tctttgaaag
     5581 gtggtgaaaa catgtgagct tatttgatat ctttaagcag tcaataagaa atgaagagcc
     5641 ttctgagtgg ataccggatt ttgtatatgg agaggaagta tctgctcggg catacctcaa
     5701 aattatggca aaaaatactg ttttagattt tgttgcaaga actatgtcaa cgttagaagt
     5761 taagtttaaa aataaagatg ggacatcaga ctgggattat attctaaatg ttcgacctaa
     5821 ttctgatatg tcagcagcta cattttggca aaaatttttt tatcggctct tagatgacaa
     5881 cgaagtgtta gtaatattta cagatgataa tcaattgctg attgctgatg attttagtcg
     5941 aacggagtat gccgtgtatg atgatgtttt tacaaatgtg atggtaaaaa attttgtctt
     6001 tgaacgttca tggaacatgt ctgacgtcat ttatatggaa tataataacg aagaattaga
     6061 aaggtttaca cgaggtttat ttgaagatta cgccgcgttg ttcggacgga ttctagaagt
     6121 tgcaatgcgt aacaaccaaa ttcgtgggtc tgtatccatt gattcaacgg gaacggcgaa
     6181 tgatgaaaaa ggaaaagatg gtaagactag atctcaaaga ttacaagaat atattgatag
     6241 gatttggaac tcttttaaaa ctaactcagt tgcaattgtt cccaaagtga aaggctttga
     6301 gtatgaggaa tacaccaata aacaaggcgt atcgaatcaa tcgctagatg aactgaataa
     6361 gatgaagtcg tctttaattg atgatgttgc caacgccata ggagtaccta cggcgcttat
     6421 ttatggtgaa aaagctgaac tggattcaaa tattaaagct tttcgtaaat tgtgtattgc
     6481 tccactagtt aaaaaacttc aagacgaatt ggtggcaaaa ataattagtc gtcaagagta
     6541 caaaaacggg gaacgtatag aagttaaaaa agttctccaa aaagatctat tagagtatgc
     6601 tactcaaatt gataaaataa catctggtgg tgcgttttat attgacgaag ttcgtgatga
     6661 agttgattat gatgagttgc caaataatga aggaaaaatt cgtattcgta cgaaaaacta
     6721 tgaagaaact gtgaagggag gtgagaacga caatgccgaa agctaaaaaa gtaccatttc
     6781 agtttactaa cgaaattcag aatggtaagc acattctcac cttgagtgga aatgtacaga
     6841 aaaaatattg gcgtgatgat gatgtcataa acgctaaaga tattcgtgac tctctagata
     6901 ctgtaaccga tgatattgta atcaagttga acagcccagg cggtgatgtg tttgaaggga
     6961 ttgagattta caattattta aaagatcacc catcaaatat tactgtagaa gttacagggc
     7021 ttgccgcaag cgcagcaacg tttattgtcg ctggtgccaa tgatgtgatt atgaatgttg
     7081 ggacttcttt gatgattcat gaagcttcta catttgcttg ggggaataaa caggacatcc
     7141 aaaaaacgtt aaacgcattg gaaacgatcg atgattctat cttagctatt tattcagata
     7201 agactggcca atctgttgat aagttgcgtg aatggatgaa tgaagaaaaa tggtttacag
     7261 cagatgaagc tgtcaaatat ggatttgcca attctgtgaa gcgtgaagaa ccacaaaatg
     7321 aacctcaaaa tattgcagcg atgattcaag atgctgttgc cgttgcaata gcgaatttaa
     7381 aggaacctgt tactaataaa atcgaacaag aatcaaaaca aaaatcatta atcgcacgat
     7441 tgcgaaaagg agaataaaga tatgttaaaa attacagata aaactgcaga tgcaaaaaag
     7501 gcatttaatg ctatttcttc gaaagaagat gcaacacccg aacaaattaa tgctgctcta
     7561 gaagaatatg taaccgccat tgcagaagat gccggcaaac aggtgcgtgc tgaatatgaa
     7621 gaattgaaaa atgtgacgga caaccgtgta ttagaggctc gtggaattcc tacactaaca
     7681 gcagaagaaa caaagtttta taatgaagcc gtaaaatctg gaggtttcga ttctgacgta
     7741 gtttggccag aaacgatttt ggaacgtgtt tttgaaaatc ttcaaaacga tcatcctatt
     7801 ttaggcctca ttaactttac accaactgta ggtcgtgtca aagtaattag agcacgtcgt
     7861 aaaggtgttg ctgtttttgg accattgcat aaagaccttg aaggccaact agatgctgaa
     7921 tttggtgcaa ctgaatttgt tcaacttgcg ttgaccgcat ttttcttaat ttctaatgat
     7981 accttagatt taggaccacg atggattgac cgttttatca atctttcttt aagtgaagca
     8041 gtacgtgatg tttgggctaa aaaaattatt acaggttctg gtaaagatga gccaattgga
     8101 ttgcttaaag atctagatgg tgcggtcaca agtggtgtat atccagataa agctagcgca
     8161 gggacattaa cttttgcgaa agagaagatt gttcttgaac ttgcaggggt gatgaaaaaa
     8221 ttatctaaat acacatacaa agtagatggt gacgatgaag gtgaagtgaa ataccgttct
     8281 gttgctggac gagtgtatct aattgttaat cctgttgatt actgggatat cgtagctaga
     8341 gttacatttg caaatttaaa caatgtgtat gggactacga tgccatttat cgctgttgat
     8401 catatcatcg aatctgtaga cgtaccagaa agtaaatgta ttgcatacgt tgataaagag
     8461 tatgaagcta ctcaatcaag agctgaaaaa gtctatgtat ataaagaaac ttttgcaatg
     8521 aaacgcgcaa ctttgtatgc tgtcgacatg cttggtaatg gctatccaac aaataatgat
     8581 gcagcacaag tatatgactt ggactttaca gcgggggagt aatgtcccct gcaattggtg
     8641 agataacacc gacgactgat ggggcagtca ttaatctttc gtaaatgggg ttagagtatg
     8701 aacactgaag aatttataaa agaatataag gcccgatttc gtattttcca tacttccgaa
     8761 gatgaggata tcgggaaaca gttagagagc gggtttgccg atataaaatc tctaatcggc
     8821 agttttgatc cttccactta tgagaaagga aaagaattag tttatgaacg tactcggtat
     8881 ctacggaacg aatcactaga gtacttttac gacaattttc aaacaatgat tatggatgca
     8941 tcaattgatc tggcaggtgc tggatatgcc aattaaatca aattataaga agcctaaaat
     9001 tgtagctggt gatctaaata cgccagttac tttttttgag tttcaaccta ataagggtcc
     9061 agaacctggt gatgaagaaa aaaaggaact gtatttttgt acagcactag tttacaaccc
     9121 atcaatgaaa gatcgtgaca ttcttggcac aaaagaaggc gttacgataa aaatacggga
     9181 tccccatcaa tcttatattc ctactaacaa acataaagta aggatattag attatcgata
     9241 tggagataaa gtttgggaaa taatcgatgt ctcaccggat ttggaagaaa attcaattgt
     9301 aaagatagta ttagaattaa ttacttaaat ttggtgataa atgatgagta acgtaaagtt
     9361 tgaaggcgtt caagaaacaa tccaagcttt agaaaaaaag tatggagaaa agaaaactaa
     9421 gacattaaca aaaaaagcaa tcaatgttgg cgctgaaaaa gtagaaaaac agttgcaaac
     9481 ggacatgacc gtttttaaag acaaaggtta tacgatcgct gaggttgttc gcaagaacgc
     9541 tacgtataga gattacaagg cagaagctga aatcggttgg aatggtccac atcaacgata
     9601 tagaatcatt catttgaatg aatggggata tacacgaaag ggcaaacaaa ttagaccgag
     9661 aggatttggt gtaataacaa aatctcttaa gaattccgag ctgttgtact ttgatgctgt
     9721 aggagaggag gttaaaaaga gtttatgaaa gatatgctta atatcattta cgaggctctt
     9781 tgtttgaatg agtatattca ttcaatgacc tacaacgaga ataccgagca atatcgtatt
     9841 aaattttatg agcaacctga aaccgccgat aaaacaggtc caatgatcac aattcgtcct
     9901 gtagatacac ctaacgaagc gtaccacgga agtaacaaag agctttctgt ggagcatatg
     9961 atacagattg acgttgaatc gaaatataga gcaacatgca agcagattca atatgagata
    10021 aaaaaagaga tgaaaaaact aggctttggc caaatttccg gtcaagggtt agatgaatac
    10081 tttactgaaa caaaccgctt tgtagacgcg cgtcgttacg aaggaaatac aaaaatttac
    10141 gataccgaat attagaaatt aagacacgaa aataacgtgt ctttttttat tacccaaaat
    10201 taggaggaaa attttatgac tttagtagga ttcaaaaaaa tgacaatcgg ggtctttgac
    10261 agcacaggga agattccagc agcaaattta tatgtaattg aaggtgaaca aaacaaaggg
    10321 gcgactgtat ctgctgaaat cagtgggcta tcaaaagaac catcaaaagt ttacggatcg
    10381 aacatcgctt actatgtctc tcaaaaagga acaggcgatg tttcagctac ttttggattg
    10441 ttagatttac caacagaagt aaatgacaaa attcttggtt acaaaaccga tacaaacaaa
    10501 atcagcttct tgggagaaga tactgagcca ccatattgtg caattttgat ggaatcggag
    10561 gatttgaacg gcgatacagc aatgcttgcg atgttcaaag gaaaattcag tcgtgaatct
    10621 attaatctta acactacgac aaatgaagct ttcgaaccag aagcagaaga atatgtattt
    10681 tcagcgattg ccaatgatgt tgaaggtgat gctaaaggcc aaacagtcgc aaaatatgtt
    10741 ggagacgaag aagcttctat tactggtttg aaagagatgg tatttcctgc agcgggggag
    10801 taactagccc cgttgttgga gcaatcactc ccactattaa tggggcaaca gttgaattaa
    10861 gttaggagga taactatgcc agatacattt aaaatctata aaaaaaatgg tactgaattt
    10921 acaaaagtcg cagagggtga aagtccactt tctattactg gaattgcagc aaatactcaa
    10981 gttgctaaag gagattatca agctacgcgt gtggtcggag gtcaagaatc agtaaaagta
    11041 gatattcctg cattcaccac tcttccaatt tcagttactg gtgtgacgtt ggataaaacg
    11101 acagctgaag ttgaacaagg cggaacctta aaattaactc ctactgtaac accagcaaat
    11161 gcaacagaca aaacaggaag ctggagcagt tcgaatacag ctgtagctac ggttactgga
    11221 ggcaacgtta cagtaaaaac agacgctgaa gttggcggaa ctacagaagt ttcatttact
    11281 actacggatg gcggtaaagt agcaaaatgt actattacag ttattgaaaa agcagcgggc
    11341 tagtcttcgg actagctttt ttatttggaa aataatggag gaatctaaat ggcaaaagta
    11401 agaattgaat tgaaagaaga atcaggaaag aaagtctatg aaaacataga cacaacaggg
    11461 aaagattatc gaaaagcttt agaaacaatc aaaaagctaa atgaagacgg agtaatgatt
    11521 tggaatcgtt tagacattta tttggatttt gctgtaggta tcttcaggga tagcaaattg
    11581 acagctgaac aaattcttga tggcttacca tctgagaagg taatgagcac attggacgac
    11641 atcctaggag aagtaatggg gattgaaagt aatccagacc cagacgcaaa aaagtaacac
    11701 ccgaagaagc agaagaaatg tacttaaacc tatgcagaga gttagtgaaa gcgggatggt
    11761 cactatctga catagaagat aattcctttg aaatgatgat gaaaattgcg tgcacttcgc
    11821 ctaagaaaga gaaacaaaaa gaagttgatc ttaaagactt cttgaaatcc atgtagagag
    11881 gaggtaaaca tatggcaaat ggaaaacctt taggcaatat gattatcaaa ctagacttag
    11941 acagttcagc tttttcaaaa ggtctagccg gagctaaaaa cgctgttaac catcaaatga
    12001 aagcaatgaa atcacagatg caagtaatga actcttcagg aaattctctc ggtgctttac
    12061 aagcaaaata caatggttta ggcggagttc tacaagcaaa tgaaaagcaa gtagaattgc
    12121 tcactaaggc ttataaggat agttttgact ctaacggaaa cgctactgct tcgactgcta
    12181 aatacgctaa tcaattgaat caagcaacag ctagatcagc aagttacgaa tctcagatga
    12241 aaacaactgt tggacagatt gctcggatga aagtggaaac ggaagggttt acggggaaac
    12301 taaaggctaa ttctgatgta ttgattaatt ctgggaaaaa aattgaagcc tttggtaaag
    12361 gagtttctgg gataggatct gccctaactg ttggagtaac agctccaata gtggcaggcg
    12421 caactgctgt tactagtgca gctatttcat gggaaagtgc ttttgcaggt gtaaaaaaga
    12481 caaatgatga agttgtagac tcgaatggaa acgtagttta ttcctatagc gacttagaga
    12541 aaggtctgcg taatcttgcc acccaattac ctggtagcca tcaagaaata gcaaatgtag
    12601 ccgaagcagc cgggcaatta ggtataaaga ctgaaaacgt agttagcttc actaaaacga
    12661 tgatagacat gggtgaatcg actaatatgt cggctgaaac tgcggcaact tctttagcac
    12721 gatttgctaa tattacccaa atgtctcaaa aagactttga taaattagga tcagtcattg
    12781 ttgatctagg taacaatttt gctacaaccg aatcagaaat cactgaaatg ggattgcggt
    12841 tagcgggagc tggtaaacag attggaatga gccaaggcga aattctagga tttgctactg
    12901 ctctatcttc tgttggtgtc gaagctgaag cgggtgggtc tgccttttcg aaggtcatga
    12961 tccagatgca actagctgta gaaaaaggat ttggcgcttt tgatcagctg aaacagatgg
    13021 cagaagaaca aggtgttccg tgggttaatt tagtaaatgc tgtgagagac ggtggaaaat
    13081 cgttaaaagc agtatcagag caaatgggat tcacttcatc tgatttaaag aaaatgtata
    13141 aagaagcgga caattcgaaa agttcgcttg aaaattttgc gaatgtggca ggtgttacaa
    13201 gtgaacaatt ccagaagttg ttcaagagtg atccttcaga agcaatcatc aagttcattc
    13261 aagggctgaa agattcagaa aaacatggta cttcagcaat caaagttctg gatgacatgg
    13321 atataaaaga agttcgacta cgcgacagtt tattgagagc tgcaaatgct agtgacatat
    13381 tcaaaggagc aattgaaaaa ggaaacggcg cgtggaaaca aaatacagct ttaacaaatg
    13441 aagctaataa aagatacgaa acaactgaat caaagctaaa gatattgaaa aacgaagtga
    13501 ttgatacagc aatcgatctt ggaggtccat ttgtagacgc gttgcgtgat ggtctaaatg
    13561 tatcgaaacc attgataaaa aacttaggga atatggcgaa agctttctca gctatgagtg
    13621 atgatcaaca aagaaacatt atcaaatggg ttggattagc agcagcagct ggtccagcgc
    13681 tgaagctttt aggaactggt acttcagtaa taggtaaagt tacttcaact actggaagat
    13741 tgacgaaaag ccttgttgat ttagcagcga aagcagcaga aaagaaggct atggatgcct
    13801 ttgcatcgtc ggtaacaaca gctggtgctg cagctgcaaa gacagcagga gctggaggta
    13861 ttggcgggtt agtccctatg atgggaaaaa cagctagtgc tgcaagtact gctgcgggcg
    13921 ccagcggaat aggcgcaatg acaacgtcac taggtttgtt aggtcctgct ctacttggta
    13981 ttgttggcgt tggtggcgca ctggcagttg gctatggagc atggaaatta tttggtgaag
    14041 aagcttggaa ttcctcgcaa agagtaaagc aatggggaac agatgtcggt gaggagatag
    14101 atagtactct tgacggagta cagaagaata ttgaaggtgc aaatggtcaa ttcggtcttc
    14161 tcaaagaggg ttttactgtt gatgatgcaa ataatatggc ggctaatttc gcaaccgctg
    14221 gaacatcttt ggaaacatct ttaacgggta gaataaaggc attagacgat gcactaaaag
    14281 ggctacctca atcagttcag gatgccatga aagaagtagc tgaatcagaa gaagaaacct
    14341 tagggcaatc tctttctaaa ataaaagaca acaatgaaga aataaaaaaa atcagagaaa
    14401 gagccgcaaa tcagaacaga gacttgactg tttcagaact acaacagata caggatctaa
    14461 tgaaaggaac ttctcaagcg tatgtggata cgctcgatgt ttctgctaaa gacagaaaaa
    14521 agatactatc agccatgaat ggtgatgttg cacaagctac agaagaagaa gcgaagacgt
    14581 ggttacaatc tttagcgaaa caaaaagctg caactaccga aaattataga caaatgcttg
    14641 aagaaaaaaa ggcggcttta aaggaaaagg gagtaagcaa agaattcatt gatgcattag
    14701 aagatgaaat gaatgattac atcaaaacta caaatcaagg attcgatagc caaatagctg
    14761 ctataacaaa taaatatcca caattgatta acgaagtgag tttatcaaat ggtcaattat
    14821 ttagcaaaac ggcagctgct atggacaata ctggtcagta tacaaaagtt tttctcgaaa
    14881 gtaatgaaaa gattttagcc caagcgaaga aaacatcgga tgaaatagca gataatgccg
    14941 aaaaaaatgc agaacaactt ggtatggtag ctgatgaggc tacgcatgct ggatcaactt
    15001 ggaatggctt agttttagac gaaaaaacag gaaaagtaaa aactaatgta caagaagtag
    15061 tgaatgaagc aacgaaggat ttcactaaat ggaatgaatt gaaacctgtt gttcatgatg
    15121 cgaaactaac aacgaatgca aaagatgtca tttccatagc ggcagtacaa aatggctatt
    15181 gggatgatat ggaatggaaa gaaaaagaag cattattaga agatgagtgt tctaaaaatg
    15241 tatacaaggc tttagaaaat gctggtaaat ggaatgagtt agatattcct tctaaagaag
    15301 caatcctaac ttcaaatact cctgaagtta tgggtgaaac tctcatgaat ttgggattat
    15361 gggatactta caaacttcaa gtgaaagatt tagatttgaa tgcgtataaa gtatacgatg
    15421 ctatcgatgg atcagaagaa aaattaaata gttgggctca attagataac cccacaaaag
    15481 aattgttatt agataactca gatttttcta caaaactttt taattccgaa gaaatgttta
    15541 aacgttggaa tcagttatca tctaatgcaa agtttctttc ggctaataat actaaattta
    15601 cagaggttct tttatcatct gacagaatgt ggaatcaatg ggtaaacctt cctgataatg
    15661 agaaaagatt acttgcagat aacgaagatt tagctaccac tgttttatct tcagaagaaa
    15721 tatacggaag atggactcaa ttaccagata atcaaaaata tatgttagct gacaatactg
    15781 acttagcaaa caaaatattc gcttcagaag aatcatggaa tgcatggaaa gcaattccag
    15841 acacagttaa aaacttgctt ggtaataata ttgatttact ggctaaagtg aaagacggga
    15901 ctgtaagtgt agaagaatat aacaaagtta tgccacttct taaaactcta tatggagata
    15961 acgttaattt aaaagaggca atcagtgggg ctaaaagctc tatagagaat tacaacaaaa
    16021 atatttttcc tagtgataaa actgcagtag gacacgatga ggcttcacaa gctgctaaag
    16081 atgcatttga agcgttcaac atcttccaaa caaaaatacc agataaaatt actaaaactg
    16141 tatcagctga ctttataggt cctatgccga aaaatgcgaa aggaacaaac ttccatcctg
    16201 gcggagcagc tatggtaaac gatcaaaaag ggcctactta tgaagagctt ataaccttgc
    16261 caagcggaga aacttttata cctaaaggac gtaatgtcgt tttagactta ccaagaggtt
    16321 cgaaagtact gaacgccact aaaactaaac gtctagtgcc taaatatgct gatggtatag
    16381 gaaacataac gacggtttca tctacgctaa acttagatgc tttaatttta gctattaacg
    16441 agctaacgac ggtattgaga actcaactga tggcaggaac gactgatgct aaagatacta
    16501 aactatcaga tgaaacaaaa gttgccgatc cgattattcc ggacagtctt tctgaaaaat
    16561 cagatcagta tctaggaatt ggagctcaat ggttaactaa tctaatgaat ggttggaact
    16621 cagcggttcc tcaatatatg aatagtgaaa tggtttttat tacaaactat ctcaatgcgc
    16681 taaaacttca aaacaatcct aattatacgc aaggagcaac atggaataag aacttactga
    16741 atggatggaa tagtttaact gggaccttta ttgctacaat caattcattt tgtaatcaag
    16801 caatggtcac gcttagaaac tacaacactc ctatgtacaa caatggccga acgtggcagc
    16861 aaaataatct taatggttgg aattcattgt atggatcatt catagcccgt gtaaatcagc
    16921 ttggtaacga ttcgattaac aaccttcgtt ctaaaagtgg tggtttttac aatgcaggga
    16981 cattcttgtt gcaatcttta ataaacggaa tgaattcgtt aggtaattct ctttctacta
    17041 caatgaacaa tgtcgctaat acaatggttg gtggtatagg caaaggagtt aatggcgtca
    17101 tatcgggagt taactatgtg cttaaggaag tcgaatcaag taagaatatc ggaaactggc
    17161 caattccaca atacgctaag ggaactgatg gacatcctgg tggacttgct atgattaatg
    17221 atcaaaaagg cctagttcac gaagaatatg ttcagatgcc agatggacgt agttttattg
    17281 ccaaaggtag agatcttctt gttaatttgc ctaaaggtgc tcaggtctta aatgcttctt
    17341 taacaaagaa attaaaggaa cgtttaaata ttccacacta cgaaaaaggt attggcaatt
    17401 tagatattgt tgatttgtta gatgatgaaa aaagaatgtt agaattctta actagcaaag
    17461 ttgatttttc aggcattaat gaaccatggc ttgatatgac taaatcagga acgtcattaa
    17521 tgtccaaagc tgcaaataca atgcttcaat caaaattaag tgaattcttc actcatggca
    17581 attttgatgg tgcagtaaat gccaatggtg tttatcaata tttagttgat gtggcacaga
    17641 aagtaatggg taagttcccc ggacttacag taacttctgg atatcgagca ggagatgcct
    17701 actatcacgg aaaacggcaa gctatcgact tggcttatcc aggtatttct ggagatccga
    17761 gatatacagc agcagcaaat tacgcttttg aaaaattccc ttcaaaaatt gcgtatgtca
    17821 ttacgaatgg acgtgtacgt gaccgcatgg gattatctgg aacaaggtca agcggacaat
    17881 ggacgaattg gccagatggc gatcacttcg atcatattca tttaaacggt tcgatggggt
    17941 ctggcgatat attcaatgga ggagttggag gaagtggtgt tgcacgctgg cgttcttatg
    18001 tgtctaaagc attgaaaatg aatggattac cagctacagc agcgtatata aatgcctgga
    18061 tgtcccaaat ccaaacggaa tctggtggta atgagaaagc aatcggcggc aatgatgggc
    18121 tcgccgaagg caatgcaaca ggattacttc aaacgaaacc cggaactttt gcagctaacg
    18181 cttttcctgg tcacggaaat attatgaacg gatttgataa catgttagca gcaatcaatt
    18241 atgctaagaa acgttacgga gttgcaggta tgttgcaagt tatcgggaaa ggacatggat
    18301 atgccaatgg cggcttgatt accaaagacg gactatatcg agcaggagaa ggaaataaac
    18361 cagaaatggt tattccattg acaagaaaaa caagagctat cgaacttatg ggtcaagcat
    18421 tagccttcct ttctggaagc aataaaaaag aaacgaaaag tgagattaat tctgtagata
    18481 atagacatat cctacaagcg atagaaaaac agtctcgtat gacaaatgat ctgctatcat
    18541 tattactctc gttttttaaa agtaacaatc gatcagataa agaactagct ttagatattc
    18601 agaaaatttt aatcaggagg acatagtatt tgaggacagt aatattaaga aataaattga
    18661 atgaagaaat tgatttgtcc acagaagatt attttgctac tgaaatgtcg aatctaggtt
    18721 ttgaagttac aaaagagcat attggacaat gggggagttt cagagaaata ggagagagta
    18781 ttgaattgtc tgaatttcaa tcatcagtta tgatttctgt tcacggattt agagaacaac
    18841 aattatatgg ttcattagtt gaatttctgg ctaatggacc ttttgaacta gaattttctt
    18901 ttgattcaga aacaatgatc aggaaatgta gtttgaaatc attatctaaa acagaaattg
    18961 attcccaaag ctcattgcta acagatactt tgaatctata ttttacttca agctggtatt
    19021 ctattaagcg tgaaaaatta atacagaatt caacttctct caatacacgt ggaaaagttt
    19081 actcatttgt tcgaccatat gtctacacca aaaatgtatg ggaaaagaaa ggtgtattta
    19141 agttcagtaa taattctgta tatttgacaa atagtacaga gcgaatgtct ccgttaaaaa
    19201 taaggattat tggagagtgc tcaaatccat attgggaagt tatccaaaac tctaaagtag
    19261 tagcttcaga tggctatttt attaatatga aagacacaca aatattagaa gtgtctagtt
    19321 tgctagaaga ccaaacggca attttaaaag atgtaggtgg agttgaatct tctgtttatc
    19381 aacaacaaga ttttactaaa tcaaacttcg tccaagcgcc tctaggagaa ttcagcgttg
    19441 tttttcatgt tggacatgca aatgtagaga ttgaacttta tgaggagcgt gatctatttt
    19501 gattttagcc attaccctct ttcaccgaga tatgaattta tataacgaac gtttaatttc
    19561 gactgagttc gaatttggaa cggatgaaat tggagaagaa gtaagtaatt ttattacaga
    19621 taaatatgtc catgtaaaat caggagattt tctactagca aaatttattc ctagcgggaa
    19681 gattgcttat tttggagtga ttagctcgca agaagacaaa aaaataaatt gcaaaggatt
    19741 actcagtttg gccgatagcg agataccgac tgtaaaaata tcaggtgcta attatgaaga
    19801 gcatattcga cgactgattg aatactactt attgaacgat ccaacaaaac aactaaaaaa
    19861 tattttggag gtaaaaacag aaagcgttac ttctcattct tatcaagctg ttgacacaaa
    19921 taagagacga ttgaatgcat atataataaa tgggttcaaa aaatataatg ttaaatggta
    19981 tgtcaaagag attaaaggta aaaaaatata tactggtatt cgagcagttg atgattcaat
    20041 ttatattaaa gataattcat ctgagtttaa tgactgggat gtttttgtgc aatctcctgg
    20101 accagggaac gagaacaaat tattgatcgt agataaagca atgaaagata tagaaaatcc
    20161 cttggtattg tctacgtggt atttagatga cgaaaataat cttacgcaag attcaacgaa
    20221 taagaacatt gcattgccta ccgttaattt agtaaatata tatgatcaaa ctcaagaaga
    20281 tagaccttct tatgaagagg tagctaaatc ggagcttaaa ggcaacgcct attcacatga
    20341 aattaaagtt aatgttgtgc taaatgctag aaatatcaat gtagaaaata tcgaaacagg
    20401 aatgttagcg actattgttt acaaagagca gacttacaaa tctgttctaa ctgcctggcg
    20461 aatatccagc agtaaaaaga atatagaatt gacatttggt aatgtccgaa gtcgttttat
    20521 ggattatttt gaagataatg ggggataaaa aatggttagc aatgtggatg gatatcaatt
    20581 tgaaaacgtg aaagtaagcg cagaaaatga tgctagactt tatcacgttt tatataatcg
    20641 gaaaaatcag gttattgatg gttacgatca gtctatgaat ttatcttcaa gcggattaac
    20701 agtaaaagtt gctgcaggag cagcgattat tcaaggtcgt atggtcgttg ttcgacaaga
    20761 agaaagtata acagttccag caaactcaag tggttatata gcattaacag tggatttgac
    20821 acaagaagtt atacctggat ctattcttcc agaatcggaa gaatatgaat ggactaataa
    20881 tcaagtcaag ctagaattta taacaaaagt tataaaaggt aatttaaaca atggtgataa
    20941 ggtctataat ttaccactat gctcagttaa ttctactgga tcaactgttt caatctcaaa
    21001 gatatcggat agttacgagc tgactctctc taaaggagaa attttgtgga gggggactgc
    21061 gttaatgcat gatactcaaa ctgtccaacc ttcaaaaaaa atttggcaga cagtttcagg
    21121 ttttttgtta gtatggctcc catacgaaaa cgggcaagca attgaggata gatatgtaac
    21181 tacgcctttt tataaggagc gtataacttt tactaatgtt ttaggagaaa ttgtttcagg
    21241 atttgatgac tatcacaaaa agtggtttag taaacgaata aactataatt cgaatactaa
    21301 tatatttaca ggtgcagcaa gcaatgcaag tggagataac gcaaatatgg tgcttaggta
    21361 tatagtttct ttttagttag gaggtatgga agagtggcaa atttagaaat taaattatct
    21421 gcaaataaga gacagcctta tctacgtcac cgtgttgttg gtagagttgg tgatgggggg
    21481 ctcacaacaa tcaatgtaca acttcttgag gaagatgaaa ttacaccttt tgtaattaat
    21541 ctaaacggta ctttgaaatt tgtgggcgaa gtttcaaacg gtaactatac cgagggagaa
    21601 ccagaaataa tcgattcgac taatgggtta attagttaca cgttcactaa gtcaaatttc
    21661 agcacgagtc atcaattcaa acaagcatat tttgaatatg tagatcctaa cggcaaaaaa
    21721 gtaacttttc agaacttcat catagacgtg ttagaacgag tagatattaa ttcggagcaa
    21781 gcgaaatact atatttcttc attggaaaaa ttacagagtg aaatgcaaac cactttcaat
    21841 cagttcatta gtgataaaca ggtccaatac gatcaaatct actcgaaata taacgaatta
    21901 gtaagattga taaatgaatc agataagcaa gtaaatgatc gtattgataa ggcaaataat
    21961 cgtattgatc aaaccaatca gcaaatcggc gatctcggca agctgaaaaa gatgtacagt
    22021 aacagcattg a
//
LOCUS       DAYYFU010000041        21988 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000041 DAYYFU010000000
VERSION     DAYYFU010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.042"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..395
                     /locus_tag="ACHA34_001839"
     CDS             <1..395
                     /locus_tag="ACHA34_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM8792457.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="ACHA34_001840"
     CDS             398..1147
                     /locus_tag="ACHA34_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8792458.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="ACHA34_001841"
     CDS             1159..1428
                     /locus_tag="ACHA34_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792459.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="ACHA34_001842"
     CDS             1433..1597
                     /locus_tag="ACHA34_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792460.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="ACHA34_001843"
     CDS             1590..1892
                     /locus_tag="ACHA34_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792461.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="ACHA34_001844"
     CDS             1889..2050
                     /locus_tag="ACHA34_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8792462.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="ACHA34_001845"
     CDS             2047..2352
                     /locus_tag="ACHA34_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792463.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="ACHA34_001846"
     CDS             2352..2708
                     /locus_tag="ACHA34_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFM8792464.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2668..2913
                     /locus_tag="ACHA34_001847"
     CDS             2668..2913
                     /locus_tag="ACHA34_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792465.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="ACHA34_001848"
     CDS             2910..3329
                     /locus_tag="ACHA34_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFM8792466.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="ACHA34_001849"
     CDS             3326..3883
                     /locus_tag="ACHA34_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFM8792467.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="ACHA34_001850"
     CDS             3880..4176
                     /locus_tag="ACHA34_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792468.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="ACHA34_001851"
     CDS             4253..4666
                     /locus_tag="ACHA34_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8792469.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="ACHA34_001852"
     CDS             5012..5386
                     /locus_tag="ACHA34_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792470.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="ACHA34_001853"
     CDS             5423..5590
                     /locus_tag="ACHA34_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792471.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="ACHA34_001854"
     CDS             5616..5960
                     /locus_tag="ACHA34_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM8792472.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="ACHA34_001855"
     CDS             5965..6246
                     /locus_tag="ACHA34_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792473.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="ACHA34_001856"
     CDS             6349..6663
                     /locus_tag="ACHA34_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM8792474.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="ACHA34_001857"
     CDS             6641..8335
                     /locus_tag="ACHA34_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8792475.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="ACHA34_001858"
     CDS             8355..9533
                     /locus_tag="ACHA34_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8792476.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="ACHA34_001859"
     CDS             9496..10182
                     /locus_tag="ACHA34_001859"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM8792477.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="ACHA34_001860"
     CDS             10182..11342
                     /locus_tag="ACHA34_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8792478.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="ACHA34_001861"
     CDS             11352..12227
                     /locus_tag="ACHA34_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792479.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="ACHA34_001862"
     CDS             12224..12535
                     /locus_tag="ACHA34_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792480.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="ACHA34_001863"
     CDS             12525..12878
                     /locus_tag="ACHA34_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8792481.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="ACHA34_001864"
     CDS             12868..13269
                     /locus_tag="ACHA34_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792482.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="ACHA34_001865"
     CDS             13262..13666
                     /locus_tag="ACHA34_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792483.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="ACHA34_001866"
     CDS             13678..14286
                     /locus_tag="ACHA34_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8792484.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="ACHA34_001867"
     CDS             14306..14668
                     /locus_tag="ACHA34_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792485.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="ACHA34_001868"
     CDS             14671..14853
                     /locus_tag="ACHA34_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792486.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="ACHA34_001869"
     CDS             14870..18289
                     /locus_tag="ACHA34_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8792487.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="ACHA34_001870"
     CDS             18340..19077
                     /locus_tag="ACHA34_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8792488.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="ACHA34_001871"
     CDS             19087..21378
                     /locus_tag="ACHA34_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8792489.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="ACHA34_001872"
     CDS             21402..>21988
                     /locus_tag="ACHA34_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM8792490.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3245 c         4705 g         6105 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatttg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DAYYFU010000042        21581 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000042 DAYYFU010000000
VERSION     DAYYFU010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.043"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            262..963
                     /locus_tag="ACHA34_001873"
     CDS             262..963
                     /locus_tag="ACHA34_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8792491.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1572..2588)
                     /gene="tsaD"
                     /locus_tag="ACHA34_001874"
     CDS             complement(1572..2588)
                     /gene="tsaD"
                     /locus_tag="ACHA34_001874"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFM8792492.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2585..3049)
                     /gene="rimI"
                     /locus_tag="ACHA34_001875"
     CDS             complement(2585..3049)
                     /gene="rimI"
                     /locus_tag="ACHA34_001875"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8792493.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(3056..3598)
                     /gene="rimI"
                     /locus_tag="ACHA34_001876"
     CDS             complement(3056..3598)
                     /gene="rimI"
                     /locus_tag="ACHA34_001876"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8792494.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3582..4304)
                     /gene="tsaB"
                     /locus_tag="ACHA34_001877"
     CDS             complement(3582..4304)
                     /gene="tsaB"
                     /locus_tag="ACHA34_001877"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFM8792495.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4495..5475)
                     /locus_tag="ACHA34_001878"
     CDS             complement(4495..5475)
                     /locus_tag="ACHA34_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8792496.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5499..6983)
                     /gene="galT"
                     /locus_tag="ACHA34_001879"
     CDS             complement(5499..6983)
                     /gene="galT"
                     /locus_tag="ACHA34_001879"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFM8792497.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6995..7984)
                     /gene="galE"
                     /locus_tag="ACHA34_001880"
     CDS             complement(6995..7984)
                     /gene="galE"
                     /locus_tag="ACHA34_001880"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFM8792498.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8232..8399
                     /locus_tag="ACHA34_001881"
     CDS             8232..8399
                     /locus_tag="ACHA34_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFM8792499.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8461..10272)
                     /locus_tag="ACHA34_001882"
     CDS             complement(8461..10272)
                     /locus_tag="ACHA34_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFM8792500.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10269..10634)
                     /locus_tag="ACHA34_001883"
     CDS             complement(10269..10634)
                     /locus_tag="ACHA34_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFM8792501.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10797..11192)
                     /locus_tag="ACHA34_001884"
     CDS             complement(10797..11192)
                     /locus_tag="ACHA34_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8792502.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11210..12172)
                     /locus_tag="ACHA34_001885"
     CDS             complement(11210..12172)
                     /locus_tag="ACHA34_001885"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFM8792503.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12172..12384)
                     /locus_tag="ACHA34_001886"
     CDS             complement(12172..12384)
                     /locus_tag="ACHA34_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFM8792504.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12405..13103)
                     /locus_tag="ACHA34_001887"
     CDS             complement(12405..13103)
                     /locus_tag="ACHA34_001887"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFM8792505.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(13123..13665)
                     /locus_tag="ACHA34_001888"
     CDS             complement(13123..13665)
                     /locus_tag="ACHA34_001888"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFM8792506.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13797..14801)
                     /locus_tag="ACHA34_001889"
     CDS             complement(13797..14801)
                     /locus_tag="ACHA34_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8792507.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14798..15787)
                     /locus_tag="ACHA34_001890"
     CDS             complement(14798..15787)
                     /locus_tag="ACHA34_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8792508.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15784..16590)
                     /locus_tag="ACHA34_001891"
     CDS             complement(15784..16590)
                     /locus_tag="ACHA34_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8792509.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16756..17712
                     /locus_tag="ACHA34_001892"
     CDS             16756..17712
                     /locus_tag="ACHA34_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8792510.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17789..18307)
                     /locus_tag="ACHA34_001893"
     CDS             complement(17789..18307)
                     /locus_tag="ACHA34_001893"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8792511.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18395..18544)
                     /gene="rpmG"
                     /locus_tag="ACHA34_001894"
     CDS             complement(18395..18544)
                     /gene="rpmG"
                     /locus_tag="ACHA34_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8792512.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18772..19218
                     /locus_tag="ACHA34_001895"
     CDS             18772..19218
                     /locus_tag="ACHA34_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8792513.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19413..21308)
                     /gene="asnB"
                     /locus_tag="ACHA34_001896"
     CDS             complement(19413..21308)
                     /gene="asnB"
                     /locus_tag="ACHA34_001896"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFM8792514.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6505 a         4564 c         3710 g         6802 t
ORIGIN      
        1 gaaaaaattt ttttgaataa attaaaaaaa taaattatat tatcgttgtt agcgtttact
       61 atatgtatta ttgttgtttt ttatacacaa atcattcgct tcaaaacaaa ttattcaata
      121 ccattgtata ttttttacat aataacaaat tatatttgta aagtattaaa acttgtttct
      181 aatgttggta tgttaaaact ttacaaattc tttaaaaaaa gtgacaataa atatagtgaa
      241 caataaggag aggacttttt aatgcgtgtt aataaaatcg attttcactt ttcaaaactc
      301 agatatcaac ctgattttga acatttctcg gatgaagagt tccaacagat caaggaacat
      361 actgttcttc gttcttataa aaaaggacag atccttttcg acgatggtga tcccagacaa
      421 cggttgtata tcttgaccaa aggactcgtt cgtttagaac gttatgatga aagtgcaact
      481 tattactact atgactatgt gaatacaaat aacctttttc ctatgtcagg aatgtttgga
      541 gaagagacct acacttatac agctcaagcg atgactgata ttgaaacatt ctaccttcct
      601 gtttcctact atgaaaaatt agcacgaaaa aatcccgaac agttgatcta tatgatacaa
      661 aaaatttcta aagtagttga aatacatgaa ttgcgtatcc aaatcggatt gacctctagc
      721 gcctttgacc gggtaaaaca aaatttattc attttgaaag aagaattggg cgtaccaaca
      781 aaagatggtc atatcaccat tccttatccg atcaccttaa aagaattagc agtgaatagc
      841 ggcacgacac gtgaaactgc tggacaagta atcaaacaac tacgtgattc cgggctgtta
      901 gattatgaca aaaagcgttt tactttctgt aagacagcta ctactccagc tatgagagaa
      961 taacgcgaac aaaaataagc agtgaccctg ataaatatca gggtcactgc ttattttcct
     1021 ccctaaaact tctgaaagat ccggcagttt tagatcactg gacaagactc aaagattttc
     1081 tatgtgtgtt tcagttatgt aatattcact gtttgcaaaa aatactgtta gattaactag
     1141 atgaagcgtc ttgtcctcat ccgatctaga ttattcgata ttcctcgaca ataaacgatt
     1201 actcagtgag ggcaatcaga ggaacggata atgataaata agctgtaaaa aatagcaaaa
     1261 taacaataat gattaaaagt atgtaaaaaa tagggctttg acaaaaacgt gtcacagccc
     1321 tatttcgatt aaacggtgtg tacctgtcaa ctcttcggtc acaagtcata atgttcataa
     1381 acatgaggag ctgttttttg aaattgttcc ttgtgaatca gagttaaacg acaggcttac
     1441 gaactcttga ttaaacggcg ttcaaaagct tgcatacgcg gtaataagaa cagccaggtc
     1501 aaaaacatga gaagccgttt ttcctgggct gttcttatta cttgatgcag ggcagctttt
     1561 tcacaacctc tttattcaga taaaaccaag cttggatcag cattcaactg atagctagca
     1621 aaatttcttt tttgcatttc cacatgtgct gctgctccaa tcattgcagc attatctcca
     1681 caaagtcgaa gagggggaat cactagttct acctcaggga gtttagctgc caaagcatcc
     1741 tgcaatcctt cacgcaaccc ttgattggcc gctacacctc cagctataat cagttgcttg
     1801 actggataat tttgacaagc tcgaagtgtt ttagtaatca atacatcgat cacacttgct
     1861 tgaaagctag ctgctaaatc atttttgtct agatcttctc cacgctgttg tgcattatgc
     1921 accaaattga taaatgcact ttttaatccg ctaaaactga aatcataatt atcttcatgg
     1981 atcatcgctc gcggaaaatg gtaattatct tttccctgat gagctagctg atcgatttct
     2041 tttccgcttg ggtaagaaag acctaatact cttccaactt tgtcataagc ttctcctgct
     2101 gcgtcatctc ttgtctcccc aatgatttca tatgatccat cttcttgcat atagactaat
     2161 tctgtatgtc ctccgctaac aagaagtgcc atcaatggaa actggaatgg tttaaccaga
     2221 cgggctgcat agatatgccc tgccatatgg tttacaggaa taagcggcaa ttggtgtgcc
     2281 caagcaaaag ccttagctgc agaaatacca atcagcaacg agccgacaag tcctggtcca
     2341 taagtgacag cgactgccga taaatcttct gccgacacac cagcttctac cagtgcatct
     2401 tccaagcata aggtgatttg ttctacgtga tggcggctag ctacttcagg tactacgcca
     2461 ccaaaacgtt tatgactgtt tatttgagaa gcaacgatgt tagataagat ctctgttcca
     2521 tttctaacga ccgctacact cgtttcatca caacttgatt caatcgccag aatcaattct
     2581 tcattcattt catcgtctcc gtttttaact ttctgctcat aatgatcgca tcttccgtcg
     2641 gattgcgata atatgatttc cgtttaccta atatacgaaa cttttctgat tcataaagtt
     2701 ttcttgctgc ttcattagat agacgtactt ctaaaaaaac atgcagcgaa tcttttattt
     2761 gttccgtaat cagtaattgt aacaaatgcc gtgcatgccc ttttccttgt tctgaaactg
     2821 caacagcaat atttgtgatc tcgacttcat ctattacttt cgagtatcca ataaagccac
     2881 agatctggtt atcttcagct aaaagcagat aatcagagtg aggctgttgg atgtctgcca
     2941 aaaactgcat tttcgtccaa ggtgaaccat gtggataagc attttcgcta atcatccata
     3001 cgcattctgc caagtacgct tcatcaaaat ctttttttct gcaaatcaac taatctcact
     3061 cattttctaa aagttttatc atatccaacg catcttcttt tgtttcagtg taatatcttt
     3121 ttttgatcgt tgttgcttga aaccccaatt tccgataaag tctttgtgca tcttgattac
     3181 tcatacgcac ttctaatgac atcgtctcac agcgattcat tatagcaaag ttctcgattt
     3241 cgtcaataag cagcgaaccg attcgtttac gctgatatgc cggtcgaacg gcaatattcg
     3301 tgatatggca atctaaaccg aacgctcttg aaccgataaa accaatcatt cttccattat
     3361 caaagatacc tagatataga tgaggaagcg gtgacttcaa ttctgatata aaggcacttt
     3421 ttgtccatgg caattcgcca ttatagactt ctcgttcgac atccagcagc tctttgatat
     3481 cttctgtagt caattctctt agtacgtaac gacgatcgat ttgttcgact gttttttctg
     3541 tataaatcat tttatcttga aaaaagcttc tggcatacgc tctaaatttt ttcaacataa
     3601 ctttcgtctc caggtgtgtg cgtctccaac catttttctt ctgcttctac tcgttttaaa
     3661 tagcgaggaa gaaaagcttg tttctctgca actggttcaa ctaaagcacc taattgagcc
     3721 acagttacac cacttggaat atcccatgct ggaactgtat taatcatcgc actaggcaat
     3781 atttccttta tttcagagaa gaatttgtga caatcactcc cgacgaaata aatagcttca
     3841 ttcagctttt gtagttgtgt gaacaaatca gaagcagaaa tatgtacatc ttcaagtaca
     3901 gtttctaatc tattttcttt ccagcggtat gccccagcgt atacattttt tcttcgagca
     3961 tcaaacatgg gcacaataat attatccaca ccgatgcaat tagccgcgat ggttttcaga
     4021 ctagatactc ctactagctc tttatctaat gtataagcaa tcgtttttgc tgtcgttaca
     4081 cctatacgaa gccctgtata agaaccggga ccatcagaaa ccgcgattcg gtcaatgtct
     4141 tttggactta tttgaagatc tgcaaacaat tggtcgattg caggcatcag tgttacactg
     4201 tggttgcgtt tgatatttgt ttgtatttgt ccaagaattt gttcgtcttc taccacaccg
     4261 atcgccaaag tttggtttgc agtatctatt cctaatgtaa tcatatattt gatcctttct
     4321 gctatgacat tctcgccttc cattgtagca tactttttta ttctgaggat gaaaatacca
     4381 aaggcaaaac tagtacaata atacatgatt atctgctggc gggcaaattt tgccccttcc
     4441 tgaaacacaa aaaagctgcg gtatcactcc attttcccac agcttttcca tcttttattt
     4501 cgttgattct cgaataatca actctgtttc cacaataatc tttcttgaaa ccggtgcttt
     4561 ggaattactt aattgaagaa tcgtctccac tgctagctct cccatccatt cggtataaac
     4621 ttttacagta gaaagcgagg gcgttacata tttcgctaca ctgacatcat tgaagcccat
     4681 cacagaaata tcttctggta ccactaaacc tttttcttga atcgctttca gtgcgccaat
     4741 cgctaatgca tcattagctg caaaaagcgc atcaggaaca tgcggattcg tttttaaata
     4801 ttgcttcact gcttgatatc ctgactcgac agaaaaatct gcttcgataa ttggaccttc
     4861 gtcgtagcct ttttctgcca aaacttgttg aaatgccata attctgggat cttttagccg
     4921 ctgatggttt ttcttcgtat attctgttcc agcaagcata gcgatatgtt gcgctttatg
     4981 ggagacaaag tgttcaatga ctttttgaac acttgactta aagtcgacaa ctagagagtc
     5041 gattccttga gaagtgccgt cagaatctac caaaagcaag gcattttggg atcctttcat
     5101 ttctaataac tgagcttcgt cgaattttcc tagtgcgatc gttccatctg ccggaagatc
     5161 actaatggaa tcccacgttt ctttcaataa agtaacgttt gcttcttctg cctttttttc
     5221 gattcctagg cgaatagata aataataaag atcttcaagc tcttccgtat cgtcgtacca
     5281 ctggatcagg cgaagcgttt gcttagtatt tgctgcattt ttacgatgtt tcgtataatt
     5341 taagcgttct gctgcttcaa aaattttttg ctttgtggct aagccaacgg aaagatcagg
     5401 atcatagttg agaactcttg aaacagtagc aggagaaact cctgccaact gcgcaatgtc
     5461 tttaattgtt gccatttgtc tctcctcctt ttttacgttt ataaaatcgc agtaaatcgt
     5521 tcaaacgctg cctgtccttt ttcatctcgc ttgaatactc cagcgtcttc cagtacacgt
     5581 gcaaaaacct gacctacaga tttttgtacg acatcggtga ccgtatcttt cgtcaaatca
     5641 ggatgttctt tttttaattg ttctgcccac ggacgatgat attcagccat ctggttttct
     5701 tctcctaata agaaatgctc tacttctact agttctggtt tcaaacgagg tggtaaaaca
     5761 gcaagcccca ttacttcgat caacccaatg ttctcctttt tgatatgttg gacatcttta
     5821 tgtggatgaa aaatcccatc aggatgttct tctgaaacat tgttgtctcg aaggacaagg
     5881 tccaactcaa agtaattgtc ccttcttcgt gcaattggcg taatcgtatg gtgtggtgta
     5941 ccatctgctg aaaaagctct gatagatact gtttcatccg aatatgcttt ccatttttcc
     6001 aatactgagt tagctgcttc aataagcaag tttttctctg ttgattgcaa acggatcaca
     6061 gacatcggcc atttcacgac tcctgcgatg acttgcggat aatcttctag tttgataagt
     6121 ttttctatcg gtgcttttgc cataggaaat tcatgacgtc ctgcctgata atgatcatga
     6181 gaaagaatcg atcctccaac aattggtaag tcagcattcg aaccaacaaa gtaatgaggc
     6241 aagacttcag taatccgaag cagtctagca aatgtttcct ttgtgatttt catcgggcgg
     6301 tgttgttctg ataaaatgat cgaatgttca ttgtaataag cataagggga atattggaat
     6361 ccccagcttt caccgtctag attcattcga ataatccggt gattggtgcg ggctggatag
     6421 ttcaagcgac ctctatatcc ttcgttttcc atgcaaagca tgcaggtagg atagtctact
     6481 tttaccgcct cgcgttctgc tgcgatttgt tttggatctt tttctggctt agataaattg
     6541 attgtgattt ccagttcacc atactccgac tctgccggaa atttgatgtt ttttgcgatg
     6601 gctcttgttt tgatgtagtc atttttctta cttaaattaa agaaatactc agttgcttcc
     6661 tctgggcttt tcgaatagtg ctgtgcaaag aatgcattga cgactgaagg tggcggtgtg
     6721 agaaaatcca ttaattgcgc ttcaaaaatc tcttgttctg ctggcaattc tgaaagaaca
     6781 ccattttctc ttgccttagc gacaagctga tccaataagt ctacagattc ttccttaact
     6841 ggtcttactt caatgctttc tagagcatct tctcctacca tccctaaaag acgattctgt
     6901 aaatataagc ggtccatttc catccatccg ccagcttcga tcgcaagtgt ggcaaaatca
     6961 caaatcgttt gactgattga catatctcat tcccctattc ttcataacca tttggatggc
     7021 tgacatgcca atcccatgct gttttgataa tcgctttgat atctgtgtat tcaggtttcc
     7081 agcccagaat ctctctcgct ttttcacttg aagcgactaa tcggctagga tctccagctc
     7141 ttcttggtgc tatttctgct ggaatttctt ttcctgtcac ttctctagct gcttctagca
     7201 tttccttgac tgagtatcct ttgctgctgc ctaaattgaa gaagttgctt tcatttcctt
     7261 ctttcaagta ttccaaagct aaaatatgtg cagcaatcaa atcttctact tgaacataat
     7321 ctctgataca tgttccatct ggtgtatcat aatcgtctcc ataaacagcc aaggcttttc
     7381 tttgaccaag agctacttgt aaaatgattg ggacaagatg cgtttctggt gtatgatctt
     7441 ctccgataga tgcatctgct tttgctccgg ctacattgaa gtagcgcaaa gcgacatagc
     7501 gcatgccata tgcttggtca caccacttca tcattttttc catcatcaat ttactttcac
     7561 catacggatt tttaggatta gctggtgtat cttctgtgat tggagactct ttcggctctc
     7621 cataagttgc tgcagtagaa gagaatacga ttttattcac gttgaactcg tgcatgactt
     7681 caagtaagat ttgcataccg tatacgttgt tgttgaaata catcaatggt ttttcgacag
     7741 attctcctac taaagagctt gcagcaaaat ggattactcc ttcaatcggt tccttttcaa
     7801 atacggaacg tagaaattct ttatcccgga tgtctccttc ataaaaatga gcatctggat
     7861 gtactgcttg tttgtgacct gtgagcaaat tatctaccac agctacttga tacccctttt
     7921 gaactagctg gtctacagcg tgcgaaccga tataaccggc tccgcctagt actaaaattg
     7981 tcataaaaaa cactcctttg attagtaaaa ttgtgtttgt tgattctata taatttaaaa
     8041 aacgaagttt acttttatgg tgaaaacaaa caaagtaaaa tttcgtaaat tcatatggta
     8101 aatttataat aaaacattta gtaaacattt tcaagggttt tttgcaaagg tttacaaaca
     8161 aacgtaaaac tagccttttt ttctcttttg ttccataata gatataacaa ataataaatg
     8221 aggtggattc aatgaaaaaa tttgtatctg gaatattagt aggaagtctg gcaacagccg
     8281 ctgccgttgc tggtttggtt gcttctgtga aaaaaacagt gatcgatcca attgacgaaa
     8341 aagaagcaat gatcgaagaa aaccgtaaaa aagcaatgcg taaacgcgtt tctcgttaat
     8401 aattatttaa aaatacgcat gaagactttc taaaaaacag ataagtcatc atgcgtattt
     8461 ttatttgtca gtcaatataa gttgtgctat tttatctgcc tgtctcagat tccatggatc
     8521 tgtataaggt ctagctactg ctgcagttag ccaatccaca ccttcaacag gtattccata
     8581 gcaataaaag tcagactgga tttcccctga agcatcaatc acgcgattcg tacctcgctc
     8641 aacatctata gcttctgaat gataacttcc tgtattatct gaaaatgaaa aagggcgaac
     8701 aagtttgttt tccttcaaat tttttatgac aggattactt gaacgagaaa agcttgtttg
     8761 tggcaatcga gcttcaatca aatagcgact gctgatttct ttatcttgta gagaaataac
     8821 atagcggttg tgcttttgct gaatatcaaa tggcggttcg atcaaatgaa aaatacccgc
     8881 atctatcagt gctgctaatt ctttcgtccg aatgattggc ggaccgatcg ttaaaaagct
     8941 atttagatga gtaaaccatc cccatagtat ttctttgtat tccttagcag aaaaaacgtg
     9001 ccattcaata gcctgccgga ttggatcacg ccagtctttt aatgcatcaa atgttgaagc
     9061 aaatgctcct gtgcaattgc ctttttccgc ttcttttatc tgcccttcaa taaatattcg
     9121 gctagcttct gaaaagggct cattctgata ggcaagagga gtttctaaat acgaccaatc
     9181 ccatctataa gggattagtg ctggatgctt tccgagacac tcctctgaag agagggagag
     9241 aaaatcatgc tcaaaatttt tctttggaat cggcaagtga tattcttcga tgattttctt
     9301 gtaataaaac aactcaacgt cttttttcag atagtcaaaa aatgtttcgc cagtcaaaaa
     9361 tcccgactga tgccattgct ccaggttttc ttttgttagt aaccgtggcc atgccattgc
     9421 tccgccttgc ttttgatttc ttcctcgagg gtaataaggg agcccccttc ttgatccact
     9481 gtatacaatc ggttccttgc ctgatggatg atagaccagt tttccctttt cgttctcgaa
     9541 gcgccctcct cttgatactg taaacaatcc gacataatca aaaaaactta agcctagacc
     9601 tcttaaaaag acattttctt taggcggtat cttcgaaaga tctacatctg caggattaga
     9661 aggaagctga tacagtaaaa catttttctt tgcgtagtct acccacactc tttcttcttc
     9721 tgtctcatca ttttcccagt gaccgcttgc taaaacaagc tgatcgatag aatagctttg
     9781 ctgatctgtc tgtaaaagat aaccatcttt tcttttgctt acttttttta ctaaagaata
     9841 aacaaactga atagaagctg gaaattcttc ttgcaacttt gagaaaaacc agcgttgata
     9901 gagtccgtat aaacatcgag tagattgctg atctttttcc agacgattga tttcttctat
     9961 aattagttgc ttatttttgg ttaccgtctg ttcgatataa tctgtagcag gtcctttact
    10021 ccattgaaac aagttgggtc cctgtgttac caatcctcca ctggacaatg tttcatctgt
    10081 aaaaagcgtc acttgttgag aaacagaatt cattaataac tccggtggct gattcaaccg
    10141 ccaaactcga cctccaggac cattgggatc gaaaagaaaa agttcaagag aatgtctgtc
    10201 ctgattatta tggagaatcc tttctgctac acttaatcct ctaggtccgg caccaatgat
    10261 ccctattttc atttgcatgc ctccatttcg ataaattttt tcaataaaac agcatggttc
    10321 tgatgttcat tttttgcacc ataaacaaaa gtaatgactg gatgttcttt cataagttga
    10381 ctgagtttaa aaatagcttg cttcttttct tcttcgaaaa tcaattcttg ctggtatttc
    10441 tttgcaaatg aagcaaatcg ttctggtaca tggtcaaaag ttttgcgtgc ttcattacta
    10501 ggtgcaattt ctttcaacca cagatctaac tgcgcttttt cttttgaaat tcctcgtggc
    10561 cagaatctat ccacgagtat tctatagcca tcctcttttt cagcatcaag gtaaacacgt
    10621 tttaatttga tcatagttgt ttccttcttt ctaccttttc gcttagctta tatatgtaga
    10681 taatagcaca aaataaaaaa aatgaacggc acaactatcc attgcgccgt tcatgtgatc
    10741 ttggtcaaaa tctaatgatt gattcggttt ttaaagctgt catacaatca tctgttttat
    10801 acttttttac ttttgatttt tccagaccat ccgtcatatc cacccttaag taaataaatc
    10861 tcttgataac ctgctttacg taatttattt gctgtacgga tgctcaaact tttcttttga
    10921 tcgtaaatat agacaggctg gtcttttcga atagatccta atgaatcttt caaaacagtg
    10981 taagggatgt ttcttgcacc taaaatatgc ccagcatcaa acacatcttt ttcgcgaaca
    11041 tcgattactt gtgcttttct cattccttct cgaaattctt cttctgtcaa agtcgttgct
    11101 gaacgtttgg ccattactcg tagatagact tcccaaagga tgattgcaag cacaatcaat
    11161 agcaaaatga tattaattac ccataaaacc atgtaatgcc tccgtttttt taagcaaatt
    11221 tcaaggctaa tgaggcagca ccaatgacac cagcttcatt accaagctct gctaatttga
    11281 tttttgttga ttctcttact tgcgggaaag taaattcttt aaaatatttc tctacacggc
    11341 tacgtaaaaa ttctcctgct gccgatactc ctccacctaa cacgatactt gacggattta
    11401 atgtattccc caagtttccg caagctaaac ctaagtagaa gcatacgcga tcaacaacca
    11461 tcaatgcaaa cggatcgtct tcttgagcca tttcaaagat atctttactt gttacttctt
    11521 ctccgttgtc tagcattgct ttcagttttg aatcccctgc gtattcttct gccaagaaac
    11581 gacctaggcg taccacacct gtagcgctag atacagtttc taaacatccg cgtttaccgc
    11641 aagtacattc gaacccttca ggatccacag tgatatgtcc gatttctcct ccagctccgc
    11701 tcacgccgtg aatcaaattt ccgccagcaa cgatacctcc gccaacacct gttcctagag
    11761 taacgaatac aacgtctgga tcgttttctc cagcaccttt ccatcgttca cctaaagcag
    11821 caacattcgc gtcattatcg atagcgaatt tgattcctgt tcctgcctcg atcagttttt
    11881 tcacaaattg aacttctgtc cagtttaagt tatacgcacc aataaccgtt ccagcttcgc
    11941 tgtccacgct tcctggggta cccatgccga ttccgataaa gtcttcggct ttcatattat
    12001 ataaattcaa acgatgattg attgattcaa tgatttcagg aacgatatgc ttaccatcat
    12061 ccaaaatatt ggtatcgatg ctccattttt gctgaatctc tccttctggt gttaaaattg
    12121 caaattttgc tgttgttcca cctaaatcaa tcccaataat cttcttgtcc attacaaatt
    12181 tcctttcttt cggctttctt caattcggtg ctctcttttt aatatgcgcc acgctgtcat
    12241 gtatgtatca tgatcaatca aacggctatc atacagatta cgcaattcga tagtcattaa
    12301 ttcaatgtca tagatgcgtg cacccacata gataaagatg ccaaattttt tgaacagctg
    12361 ctgtacatca tataagctct ccatctttaa gcacttcctt catattatac aagtaaacca
    12421 tattttttta aacccagtaa aatacatatt acaaaaagaa atacaaaaac tagtgtagaa
    12481 agaatccttt ggtgtatcga atagcttgtc tgttgtttcg gtagacccaa aacattccct
    12541 aacactaatc cgccaattag cccaccgaca tgtccccaaa catctacaga ggtatccaga
    12601 agaccaaaaa caaatgtcaa cacaataaat aaaagaaaat gtctgactag ctgctggatc
    12661 gctgggttat gtttaaaatg aaaccctaag ataaacaacg caccgaacaa accgaatatc
    12721 gaagtacttg caccaccaga cagaacattc gcttcgttaa aagcaaagct tgctgtattt
    12781 cccattatcc cgctgaacat ataaatcaag aaaaaacgcc aatggccata gattgcctcg
    12841 atttgctgcc ccataaaata cagtatcagt gagttcatta cgaaatgcat cagtccaaaa
    12901 tggatgaaga ttggtgtcac taaacgccac cattcattga agtgaacaag ataaggacca
    12961 tacattccgg cataataagg gatctcaagt cgcggaaata attccatcat gaggaagacc
    13021 gctgtctgta tccctaaaaa agcataggtc cataacggtc gtcttaacca tgcacgcaat
    13081 tttatttgct gttgataatt catgaaaacc tcccatttca ttttagtcaa taaacaattg
    13141 ttggatcttc tgatcaaagc tctccggttg ccattgttca ttcaattgtt cacgaaaaac
    13201 aaggctacat gtttttttat gaaaattatt taaataccgg tcgtaataac caccgccaaa
    13261 accgattcga taaccggctc tattgtagat aatacctggt acaatgagga gatcgatcat
    13321 ctccggcaaa tagagcttct ctgattgtgg ctcaagaaca ccaaaactcg aacgtacaag
    13381 aacggactgt tcatccattt cataaaacgc catctgacgt ttcggcaaag ttttaggtac
    13441 aactattttc ttttgttctt tttctgctcg ttggataacc ggctgtgtat ccagttcaaa
    13501 ttcattcgat aaagtcgtcc ctatcacttt tgctgacttc cagtactcgc tggcgaatag
    13561 ctgtttatag attcgctgtt ctttttcctt tttcttgcca cttttggcca gatttttcag
    13621 cttttcgatt cctttccggc gtaatcttac tttcaattgg tccaaaaact cacccttcct
    13681 tctctcataa aatggtatag gaaaaaaaga aaaatagcaa tcgaactttc aaataaatgc
    13741 ataaatagaa taaaaagaag acctagaaat gattggcctt ccttttttaa tgaatttcaa
    13801 ctgttcagcg cgtgtttttg tctgataaaa agtgcataca gaaaatatgg cgcaccaaaa
    13861 agggcaatca aaattcctgt aggtacttcc ccacttggta gaatcactct agcaaccata
    13921 tctgcactaa gcagaaaaat ggaaccgaac aaaccgctca gaacaaaggt ccagttattt
    13981 tctttacgta cgactagctt cgcagcatga ggagctagta gtcctacaaa agataaacta
    14041 cctgtcaccg ctacactacc gcttgccaaa gcaacagcta acaacaaaag gattttttga
    14101 cttttagcca cgttaaggcc taatccttgc gcgcgttctt gaccaaatga tagcaataca
    14161 atcctctgtg cataaagtcc tcctagcgga attaagataa ccgcccatgg aagcaatgcc
    14221 caaacataag gccagctcgc accccagatt gatcccgcta accatgcatt cacaaatcca
    14281 taattttcct tagataattg gatcgtgcat agcaatgtta atgcagaaag gccggcatta
    14341 acggcaatac cggacaataa gattcgattc atacccaaaa agccatttcg atcataggcc
    14401 gcaagataga cacagcaagc agctaaaaca ccacctagaa aggctacaac tagcaataga
    14461 aacggcagcg aatagtgcga aaagaaacct aaataaagca tcactaaaaa tcctgctccg
    14521 gcattaatgc ccaatatact agaatctgcc aattcatttc ttgttactcc ttgcaagata
    14581 taaccagaaa cgccaagaca agctcctgct aataaactga tcagtaatcg aggaactcga
    14641 aattctctaa caatcagcgc caaagaagat gattctttcc ctatgaacgt ggcaaaaagc
    14701 tgatccaggt aaataaaagg cgtaccaatc attaaactac ctatagacag gatgacaagt
    14761 acaaaaagca agaaatagaa cgtccaatat atttttttca ccgttgttcc ctccttactt
    14821 gtatcagtaa aaacggaacg ccaatcaatg caatgactaa tccaaaaggt gtttcaaaag
    14881 ggggattgat catcctcgct gtaaaatccg ccaacataac caatccaccg ccaactaaaa
    14941 aagagacagg tagaacataa cgatagtccg ttccaaccat tccgcgaaca actggcggaa
    15001 ccaacagccc aataaatgaa acaggtccga ctaacgaaac ggcaatggct gctaacactg
    15061 caactagaca gaaaacaatc aaccgcgttc gtttaggatg acttccaaga gctaatgcat
    15121 gtgtttcact caaatgaatc aaagatattg cttttcctaa tgaaaaagaa accaaaacag
    15181 caagtatata taaaggaaac aatactgcta attgtttcca tgtgatattg gcagctccgc
    15241 ctacaaacca aaaagcaaga tcctgctgca gattaaagaa ttgggtaagt gcttggctga
    15301 tagcagtaaa aaaagaacta atagctacgc cagctaaaat cagctgtaaa ggattcatgc
    15361 ccattttcga aaactgcaaa aaaccaaata cgagcaaagt tacagcaaaa gaacctgcca
    15421 atgacattag aaacacagaa aaatcagaag ctttaggcca aaaagcaaaa ctaagggcca
    15481 ttgccaacga agcacccgcg ttgattccta gtaagcctga atctgctaaa ggatttcttg
    15541 tcaccccttg catcaatgca ccgctcaatg caaaacaact ccctacaaga aatgctcccg
    15601 ccacacgagg caaacgaata tttcgtatga tttggtgact ttgttttgat ctatcaaaat
    15661 caaacaacgc ctgccaaaaa tctgcttttt ccacagtcgc tgcgccattt gaaagagcaa
    15721 caaaaaaaat aatcaaaaga acaaaacaga ccaaaagcag gagtctattt ttcttcatag
    15781 tcctcataat tgatcctcct gctcttttgg caacaaatca taggttagaa aaactggttt
    15841 atttgttctt ggaagcgtga caatcgttgc atcaatctca aatatttttt tcatacactc
    15901 ttttgtgatc acttcttcag gtgtaccttc tgtgatcaac ttaccgtttt tcattgctaa
    15961 taaataatct gaaaaacgtg aagcatgatt aatgtcatga atcgacatca agatcgttgt
    16021 ttgtttcatt tgattgattt cagtcaataa ctgtaggatc tctaattggt gtgccggatc
    16081 taaaaatgtt gtcggttcat ccaagatcaa gatatctgtt tcctgagcaa gcgccatagc
    16141 gatccagaca cgctgggttt gtccaccaga cagcgttgcg atatcccgca tacgaaatcc
    16201 agctagacct gtagcatcca acgcccactc aattgcttca tgatcttgcg tgtccatgcc
    16261 actgaacagt ttttgataag ggtatcgtcc gtaagatact acttcttcta ctgtcatacc
    16321 tgtagggtgt tcactagttt gtgctaataa tgcaattttt tttgcaagtt cttttgaact
    16381 gtatgtagag atatcagagt tattgacaat gatctttcct gatttcacag gtagtatacg
    16441 ggtaaaactt ttcaataaag tagattttcc acttccattt ggcccaatca aactagtgat
    16501 tttcccttca ggaatcgtaa cagacatgtt ttcaatgatt tttttgtttt cgtagccggc
    16561 tgcaatgtca gctgctctta atctcgtcat ttcacccatc ctttcgctga aaatcattct
    16621 caattatgac gctgtttcaa catttcgtca agcgctactt tcatatctat agccaaatga
    16681 agcggttgac agcgttcgaa atatcaggaa taataaccaa tgagaaagat tctcaattat
    16741 gttaggagga aagaaatgaa attaaaaaca aaagtattca cattatctag catattgctg
    16801 ctggctggag tactgagcgc ttgctccccc tcttcagata aagaaacagc tacaactgcc
    16861 aattcgacga cttcatccaa tagtactcat acagtcacag atacacttgg acataaagta
    16921 gagattccaa acgcgcccaa acggatcatc ggcagttact tagaagacta tctgattgca
    16981 ctaggggaaa aacctatcgc ccagtggact gttggcagtg gttccattca agattatctg
    17041 caagatgatt taaaggatgt cccaacgatt tcttatgacc ttccttatga gaaagtgtta
    17101 agctttgagc ctgatttgct gttgatttct tcttccgcta ctgtcgaagg cgggaaatac
    17161 gaacaatata gcaaaatcgc gccaacttat gttgtaaaaa acggaacaga tgtcacatgg
    17221 gaagaacaac taaaagatgt cggcaaagct ttggcaaaag aaaaagaggc agaaaaaatc
    17281 atttccgatt atcaaaaatc catcaagaaa actcgagaag aactagcaga taaaatcgag
    17341 aacaagacag ctgcagtgtt gtgggtgacc aacaactcag cctttatggt cagcaaaaat
    17401 cggtcaagtg gaagaatcgt atacgacgat ctggaatttg gtgttcctga tctagtggaa
    17461 gaggtctcca aaaaagccac ttctgactgg tctgctgttt cctctgaaaa gctagctgaa
    17521 ttagatgctg actacatcat ccttgtgaac agtgacaaag atgctgccat gttcaatgaa
    17581 ccaagctgga agaatctaaa agcagtgaca gacaatcatg tactgcaatt cggacctgaa
    17641 tctagttggc tatataacgg acctattgcc agtcaggaaa tggtcaatga tatcaaagaa
    17701 aaactaaatt gattttaact aaaaaaagca tcttcacgat tagtcgtgag atgctttttt
    17761 tcttattaac aaactagacg catagatttt attcttcttt tgaagcaacc ctatttttta
    17821 ctctttccca tttttgaaaa cgatgggcat atagattttc ttgatccgtt atcccctcat
    17881 actcctctat aagggcgaag tctgaaaaat caatattccc aatatacgta tcgccttcaa
    17941 acgtgctatc gatcaatgtt cgccagatcc gaccagtgta aggaagaagt tcttgaaaaa
    18001 tccgactacc acccgaaaca tagatatctt ctgagatcgt ttgactatac gacaaaagtt
    18061 catcgatcga atgcattact tccgcatttt ctttcgtgcg aaaatttggt tgcgttgtca
    18121 acacgatgat atggcgataa ggaagtgaaa gatcccccat tccttcatag gtctttcttc
    18181 ccatcactaa aattcgatcc attgtatgtt cgcgaaagaa acgcatatca tttggcaatc
    18241 gccatggtaa caaaccattt ttgccgatca gaccattttt atcttgtgcc cacatggaaa
    18301 taaacatatt atccgctcct tatgatttta gatgttttga gataaaaaaa gtctcaattc
    18361 tttgaccagc aaggaattga gaactttaaa aacattattt tgtttcacgg tgcaaagtaa
    18421 ctttgcgttc gcgtgggcaa tatttttgtt tttccaaacg atcaggattg ttacgtttgt
    18481 ttttgcttgt tagatagtta cgttctttac aagaagtaca ctctaaagtg atgtttacgc
    18541 gcatgttttt ccctcccatt tttacaagaa tcctctaaga tgactctcag acttgattat
    18601 cttaccatga ttttttctag attgctagtg tttttcacga ttttgttaag taaaactact
    18661 tgacgataaa tacaacggtt ttacttctca aacaaaatct ttttcttttt gtataaatac
    18721 ccttacactt gtttatatag attatgtttt ttaataaagg gggatactaa tatgccatgg
    18781 aacatgaagg actttcctgc atcaatgaaa aacttagata aattgacaag aaaaaaagca
    18841 atcgacatcg caaatgcatt attagatgaa gggtaccctg ataaccgtgc gatcccaatt
    18901 gccattgatc aagcaaaaaa atggtatgaa gatgctagtg aatcagaaaa acgaactttc
    18961 gaaaaagaaa aaaatccaag caagacggat gaacacgata cgaatcctcg tgctggaaaa
    19021 ctgcttgatt cagatgtgat cgtagaatat gaggaagaac aatggatcgt gaaatctaaa
    19081 ggggcaaaaa aggcaagcaa ccattttgat acaaaaaaag aagcaatcga aaaaggaaag
    19141 caagtagcac aaaacaaaga atcaactttg ttcatttata aaaaagatgg gacaaaagaa
    19201 aaagaaatcg attattgaac atatgtaaag gggttgtgac gaaaatcgtg tcacaacccc
    19261 cttatgttcg aaacaatggt gttcctctgc caactccgaa ccataagtca taatatctat
    19321 aaaacgggaa gagctgtttt ttgaaattgt ttcttatcac tcagtgttaa acggtaggag
    19381 aaaacctctt ttttattgat atttgatttt catcaaacag tcacatagtc gattgcttct
    19441 gcttgcggaa tcgtttcatc tacaaagtag actttatacc atgtcaagaa actgaagatt
    19501 gtccagattt ttctttgctc ttctttacgt ccttcgtgat ggtcatctag caacttcatg
    19561 attttttctt gatcaaaaaa ttcttttgca aaatctgctt cgaataatgc tttgacttgt
    19621 tcgtagccgt gttcttcttt caaccaagct ttgattggca cagggaatcc taatttcaca
    19681 cgattcgacc attcttccgg aagatgtttg ttcgcagctt gtctgaaaat atcttttgtg
    19741 ttgttttgat tcaataaaaa cttacttggt atttgttgag ccaatttcat gacttcgata
    19801 tccaataaag gtactcggac ttccaaagaa cttgccatag aaagtttatc tgcctttaac
    19861 aagatatctt ttggcatcca ttgatgaaga tcgacatatt gcatcttatt gacttcatct
    19921 ttgattcctt ctgttttctc gtaatgaaca gtcatgattt catccacaga tggtgctgtt
    19981 tgatattttg gtgttaacac ttctgatgct tctgattctt cgaacactcg agcatgaccg
    20041 ataaaatatt cttttgcagg agcaagtgat tcatataaat ggatccgtcc atggaagttt
    20101 ttcatttttc cgattttacg tcctaaatta tagcgtgttc cttttggtaa ttttttcaat
    20161 ccttgagcaa tcacacggat gaattttgaa ttcgtatgga atccatacgt ttggtatccc
    20221 gcaaacagtt catccgcccc ttctccagat tggactaccc gcacactttg tgaagcaaga
    20281 tttgctaaaa agtacaaagg tacgcaagaa ggatttgaat caggttcatc taagtgatac
    20341 tggatcaaag ggaatgcttt gaacgacatg tctccgtcga tcactgctgc tgtattatct
    20401 aagtccaata gttcggttag ttttctggct tcaatcgctt cattataggt tttatcatca
    20461 aacccgattg agaaagaatg atctggtttc aatacagagg ttacataact ggaatcaacc
    20521 ccgctggata agaaagaacc aacttctaca tcactgaccg tatgtgcttc aatagaagct
    20581 tgaacggttt cgtcgatttt ttcgatccat tcttgtcggg aatgcgtttc ttttgtttca
    20641 aaatctgcat cccaatattg ctgaatgtct atttttccat ctttatacgt ataataatgg
    20701 ccttcaggca aacgatagac atctttgaag aatgtttcgc cgtttaatgg agaatattgg
    20761 aaagtcatat aaggtttcaa cgcttctttg ttcaattctt tgttaaaatc aggatgaggc
    20821 agaaaacttt tgatttctga accaaacata aatgttccgt tcatttctgc ataataatat
    20881 ggtttgatcc caaagtggtc tcttgcaccg aataactcat tcttttcatt atcccaaata
    20941 gcaaaagcaa acattccgcg gatcttttgc aataattctg ttccccattc ttcgtaacca
    21001 tgcaaaagga cttctgtatc tgcatgtgtt tgaaaaacgt ggccagctgc aatcaattct
    21061 tctctcaaag gctggtaatt atagatttcg ccattaaaaa taatgatctt tgtccgatct
    21121 tcattgtaaa taggctgagt accgccctct aagtcaataa tacttaatct tctgaatcct
    21181 aaagcaacat gtttatcgat atattctcct gaactattag gtccacggtg aacgattcga
    21241 tccatcattg tattaataat tgtttttttg ttgtccttgt cgttaacaaa acctacaata
    21301 ccgcacattt aactttcaca tcctaatata aaattactgt ttatttcatt catatgcatc
    21361 ttatcataga ataaatagcg gacgaatatt ttctctctta caatttcgtc acgtgaaaac
    21421 aaaagatgaa agtttctgtt tttacagaaa ataaagtccc aatatctaat ttaccatgtt
    21481 ttcttttttg aggctacctt attctcttcg tgttaaaaaa atattagtgg atgctatttc
    21541 caaaaaaacc agcaaaaaaa gacacgacct aaagatcgtg t
//
LOCUS       DAYYFU010000043        21338 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000043 DAYYFU010000000
VERSION     DAYYFU010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.044"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..152
                     /locus_tag="ACHA34_001897"
     CDS             <1..152
                     /locus_tag="ACHA34_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8792515.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="ACHA34_001898"
     CDS             complement(230..763)
                     /locus_tag="ACHA34_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFM8792516.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="ACHA34_001899"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="ACHA34_001899"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFM8792517.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="ACHA34_001900"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="ACHA34_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFM8792518.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="ACHA34_001901"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="ACHA34_001901"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8792519.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="ACHA34_001902"
     CDS             complement(4488..5066)
                     /locus_tag="ACHA34_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFM8792520.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="ACHA34_001903"
     CDS             5388..5858
                     /locus_tag="ACHA34_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFM8792521.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="ACHA34_001904"
     CDS             complement(5966..7192)
                     /locus_tag="ACHA34_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8792522.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="ACHA34_001905"
     CDS             complement(7946..8146)
                     /locus_tag="ACHA34_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8792523.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="ACHA34_001906"
     CDS             complement(8280..8888)
                     /locus_tag="ACHA34_001906"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFM8792524.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="ACHA34_001907"
     CDS             complement(8973..9443)
                     /locus_tag="ACHA34_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFM8792525.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="ACHA34_001908"
     CDS             complement(9491..10405)
                     /locus_tag="ACHA34_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8792526.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="ACHA34_001909"
     CDS             10551..11354
                     /locus_tag="ACHA34_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFM8792527.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="ACHA34_001910"
     CDS             11532..12479
                     /locus_tag="ACHA34_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFM8792528.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="ACHA34_001911"
     CDS             complement(12547..12840)
                     /locus_tag="ACHA34_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFM8792529.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="ACHA34_001912"
     CDS             complement(12868..13533)
                     /locus_tag="ACHA34_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFM8792530.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="ACHA34_001913"
     CDS             complement(13673..14305)
                     /locus_tag="ACHA34_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8792531.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /gene="liaS"
                     /locus_tag="ACHA34_001914"
     CDS             complement(14312..15379)
                     /gene="liaS"
                     /locus_tag="ACHA34_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFM8792532.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /gene="liaF"
                     /locus_tag="ACHA34_001915"
     CDS             complement(15376..16107)
                     /gene="liaF"
                     /locus_tag="ACHA34_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFM8792533.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="ACHA34_001916"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="ACHA34_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFM8792534.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="ACHA34_001917"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="ACHA34_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFM8792535.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18270..19439
                     /locus_tag="ACHA34_001918"
     CDS             18270..19439
                     /locus_tag="ACHA34_001918"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8792536.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(19706..>21338)
                     /locus_tag="ACHA34_001919"
     CDS             complement(19706..>21338)
                     /locus_tag="ACHA34_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792537.1"
                     /translation="RWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFH
                     INRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYI
                     VTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLI
                     LLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFL
                     IIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSE
                     LLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNI
                     IQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMV
                     KEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNK
                     DEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIR
                     YSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         6584 a         4179 c         3365 g         7210 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    18181 actataagat taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt
    18241 aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa
    18301 aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa
    18361 ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat
    18421 cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc
    18481 aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca
    18541 tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc
    18601 tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag
    18661 tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca caaattgctg
    18721 gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg
    18781 cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa
    18841 aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa
    18901 ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac
    18961 ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac
    19021 tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag
    19081 agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc
    19141 tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc
    19201 gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc
    19261 tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg
    19321 atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg
    19381 atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat
    19441 atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg
    19501 actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa
    19561 ttccaaataa acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa
    19621 gatttctctt taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta
    19681 gattgctaaa atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc
    19741 aataaaaaag aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa
    19801 atgtgtatga atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa
    19861 aaggacctcc ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt
    19921 tataccggaa tacaggtaaa actattgttt tgccttttga agatttaaac gtaaatgtaa
    19981 tttcatcttt atttggagaa aagctactat aaataacttg accatcttct gtaacttgat
    20041 ggtttttcaa aagcaattga tcttttttat agcctatagg tacataatca aaattctcaa
    20101 attcttcaag tttattttcc atttgttcat tagaataagt tccttgaaaa ttcatttctt
    20161 tcaccatgtt aactactgat gaataattta aaattgaaat agctaatatt aataatagta
    20221 taaaatttct tctccttgta taagaaaatt tttccaactg tggagaaata aatagagata
    20281 ctgaaaaaga tatgaacaat gttgaaaata tcataaaacg ccaaggagct tgaataatat
    20341 ttaaaggagt attttgtaag gcaaaccatg gaaatatttt tgtacaaagt agaatagata
    20401 ttatacccat tatataagtg attttagata agattcctaa ttttttaaag ttaaatgtta
    20461 gtataattaa acaaataagc aaagttaagc caataccaaa tgatagtaat tctgaatcta
    20521 tacttccaat caaaaactct ttacccagat aaattgctct tttttgtaat atatccacat
    20581 aaggtctatt cagatcttga tacaatgtct gctcaatcat aggacctata aacgctagtg
    20641 aaagaagtaa agtaactata gcagctttta ttaatttaat aattaagaag atttctattt
    20701 tctttttccg ataaaattga ataattaaag caattataat cattaccata gtcatagcta
    20761 cagaaagaac atgagtgtaa attaataaag tcatgctaat cgccaacata acccaagatt
    20821 ctttttttga gtcgtaaagc tttataaacc cgagtaaaat aagaggaagt attgacattg
    20881 cgagtaattc ccccattgcc cctcgaagaa atatttctac actcctaaaa gaagatgttg
    20941 tatatataat agcgaaaaag gagctagtaa catgtttttt ggtaatttca tacattacat
    21001 aatgacatat aaatagagta atgagcgtaa caatataaag aaaaaggtta tagcctattg
    21061 ctaaattagt aaacttgatc aataaaaata aaggatatag agttaaccat gggtaaaaat
    21121 tatttacttg catgccatat gatctaaacg tttcaaaatt aataggggag ctaaaaatag
    21181 attctaaact taacgcacga ttaatgtgaa atgccgtatc ataaaagcca aatatttcca
    21241 tgtttttaaa tgctggaaat aaatataata aactaacaaa tgcataaact ataaatattt
    21301 ttacagaaaa aatttttttc aaattcatat accacctc
//
LOCUS       DAYYFU010000044        20485 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000044 DAYYFU010000000
VERSION     DAYYFU010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.045"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="ACHA34_001920"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="ACHA34_001920"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFM8792538.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="ACHA34_001921"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="ACHA34_001921"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFM8792539.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="ACHA34_001922"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="ACHA34_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFM8792540.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="ACHA34_001923"
     CDS             2857..3732
                     /locus_tag="ACHA34_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFM8792541.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="ACHA34_001924"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="ACHA34_001924"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFM8792542.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="ACHA34_001925"
     CDS             4629..5063
                     /locus_tag="ACHA34_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFM8792543.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="ACHA34_001926"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="ACHA34_001926"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFM8792544.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="ACHA34_001927"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="ACHA34_001927"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFM8792545.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="ACHA34_001928"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="ACHA34_001928"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFM8792546.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="ACHA34_001929"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="ACHA34_001929"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFM8792547.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="ACHA34_001930"
     CDS             13334..14260
                     /locus_tag="ACHA34_001930"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFM8792548.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="ACHA34_001931"
     CDS             14442..15335
                     /locus_tag="ACHA34_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFM8792549.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="ACHA34_001932"
     CDS             15469..15594
                     /locus_tag="ACHA34_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFM8792550.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="ACHA34_001933"
     CDS             complement(15728..16360)
                     /locus_tag="ACHA34_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792551.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16508..17050)
                     /locus_tag="ACHA34_001934"
     CDS             complement(16508..17050)
                     /locus_tag="ACHA34_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFM8792552.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17185..17574
                     /locus_tag="ACHA34_001935"
     CDS             17185..17574
                     /locus_tag="ACHA34_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792553.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17578..18342
                     /locus_tag="ACHA34_001936"
     CDS             17578..18342
                     /locus_tag="ACHA34_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8792554.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18471..19418
                     /locus_tag="ACHA34_001937"
     CDS             18471..19418
                     /locus_tag="ACHA34_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8792555.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19548..20288
                     /locus_tag="ACHA34_001938"
     CDS             19548..20288
                     /locus_tag="ACHA34_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792556.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7193 a         3481 c         4316 g         5495 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatctcca aattaaattg
    16381 ataagtatat aaagtataat aacaaattga tgagtatatg tcgatgtttt ttaatcaaaa
    16441 taataaataa aaactgtcta acatttaaaa tttaatttag atattagaca gtttttaagt
    16501 ttgtgagtta ttttccttgt cgttttctca cttttttagt ccagaaacca cctgaaatat
    16561 attttggctc ataagaaatg ataaaagcac gctcttcgat ttctttgatc aggcgataca
    16621 gttcacgttc gctttttcta ggagaaagta tttcgagaac tagccgctca ccttccagcc
    16681 ccattgcaga agtctgtgtc acaccataac cgtgttctct cagtataccc ggcaaacttt
    16741 tatcttctgc gctatttgct ggcaaaatcg ttgtgaccat gatgtatcct aatgccaatt
    16801 tgtcttcaat caaaattccg acactgatac ctactgcgta acctaacgca tagacaaata
    16861 aattcagtgg attatctaaa cgatctaaaa caagggataa gccgacgata taaatcgtga
    16921 tctcgaccat actgacaagt ggagcaatca gtcgatagcc tttcattgtc aacatgaaac
    16981 gtaacgtgtt cagagtgata taagcaaaat taatgaggaa gatcatcccc aacatttttg
    17041 gatcaatcat ttgtaaaact cctttcattt atataacaac aatatcaaaa aaaatcgtaa
    17101 gtgaagaaaa attgaaactt tatacataca aaaaaatgat aaaatgatta taatagaatt
    17161 ataaagatag gttaggtgat taaaatgaaa acggtcaatc gtgtgttttg tgccagccac
    17221 gtgctaagtc tccttctcag tatatactta ggtgtctatt cgattcaaca gccaattgag
    17281 gtagagaaca ttgtttttca gatgtcgctg acagatggtt taattttaag cgtttttttc
    17341 tttttgatac tgtttattgg taatattttc ggtagcgttg ccagtttttt gaatttttct
    17401 atttatccac tcctttcgct agcattagga gtggtgggac tttgttctct gtgcttattt
    17461 cccgagccat tcagtcctgc atttatatta tttggtacac tgaacctgtt ccagacaact
    17521 gttggagcat ggttgctatg gcgatcagga aatttaatga aaataggtga gtaacaattg
    17581 aatccaatta tattgaaacg ttctgtccat catattccag tgttagaagt tgttctgaaa
    17641 gaattaagag aggaacgtat tcccgttgtc atctattatc acggatggca gacaagtaag
    17701 gagttagttt tgacgcaagg aagaaaaatt gcgcagcagg gcattcgtgt gatcctgcca
    17761 gatgcgatga atcatgggga gcgcaaacag ccggtttctt ctattccatc ctatacattt
    17821 tgggatagta tctacggaaa tctgtttgaa tttgacacac ttatcaagca tttagaaaaa
    17881 agaggtcttc tacatgatca gcttgcagtt ggcggggttt cgatgggggg aatgacaaca
    17941 tgcgccttgt tggctaaaca tcctcatata gtagcgggcg tgtgtttgat gggttcccct
    18001 gcacctttgg aatatggaaa agaaatcgta aggcgcgcat cggaatataa ttatgtcgta
    18061 ccaaaagact attttgattt gatttcatgg gttaagagct atgatttgtc attgcagccg
    18121 gaaaagttag ctggacgtcc tctgttcttt tggcatggac aagctgacga aaaaatccct
    18181 tatatacaaa cggaacgatt tgtagaaaaa aatcggcata agtcttacgg aaaagaaatt
    18241 gtttttcgtt cctcaaaaac acagcgacat atggtacaaa taccactaat ggaagaagct
    18301 tccacttttt tagcaaaaaa tttgataaaa aagtataaat aattgaaacg gaaaatcact
    18361 catgacgata gaggattttc cgttttttat tttgggctat aataaactct gttaggaaaa
    18421 tttggtataa ttgatagtaa gaatgacaac atgttagaaa ggaagagagc atgcccactt
    18481 tagcagatgt tgcaaaacgt gccaatgttt caaaaatgac ggtctctcga gtcatcaatc
    18541 atccggaaca ggtgactgac gaattgaaag agttagtttt ctcagcaatg gctgaactgg
    18601 attaccgtcc aaatattgct gcaaaagcac ttgtcacgaa tcggtcgcag atcatcaaat
    18661 tatttatact ggaagaaatc gacacaactg agccctatta catgaatttg ctgatgggaa
    18721 tcgctaaacg gatcggtaaa gcgcattatt ctctccaatt agttacaaat gatgccttcg
    18781 acgtcggttc ttgtgatgga tacatcatca caggtgtcag aaaaaaagac ttcgattgga
    18841 tcgaccgttt ggaaaaacca gttgtcttgt ttggggaaaa ttctgaagga tatgattatg
    18901 tagacagcga taatgaatat ggtacagcaa aagcaacgca atatgcctta gaaagagatt
    18961 atcaaacgat tatttatatc ggtatcgaag tggacgagcc ttttgaaatt tcaagggaaa
    19021 ctggttattt atctgtgatg aaggaagttg atcttcctag taaaatcttg cggtttccaa
    19081 atcattcgac agaagtagaa agatatatga cagaaaactg ggcagatttt acagataaaa
    19141 cttgttttgt atgtagttca gatcggctag cactaggagc agtaagaggg attataaatg
    19201 ctggaggaca cttgcccgaa gaatttggtg tgattggatt tgatggggta tttttagacc
    19261 aaatcagttc acctaaattg acaacgatca agcaagatat tatacgtatg ggtgaagtat
    19321 gtggagaaat gttgttaaaa aaaatagaag aaaaaggggc gagtcaaggt tatcgtcgtt
    19381 tttttcctga actagttatc agagagacaa cgaattaagt cttattacta tatatatatt
    19441 tatgaaattc atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat
    19501 ctgtgctatt attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca
    19561 gacgaaaaca tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg
    19621 acaagaagcg agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc
    19681 cgtttgaaca gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca
    19741 agcgtcgtgc aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata
    19801 aaggaaaatt accggattat accggccatt accaatcaga aatcacatcg acaggacgaa
    19861 aaaaactttt tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca
    19921 agcatactac acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc
    19981 ctaaatatgt acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc
    20041 atttaatcga atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga
    20101 cgccgtatca agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa
    20161 cgttagagga aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag
    20221 aacgctcatt acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt
    20281 ttaaataagt gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt
    20341 ttccttattt tatactctat ccgaaagttt atatcgtatc ctcaaatttt tttgagttta
    20401 agcgaatttt ttctcccaat aagtaagaaa ataatggcaa aataaaaaaa caaactattt
    20461 tcgcagatcg tttgcaaaaa tagtt
//
LOCUS       DAYYFU010000045        19821 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000045 DAYYFU010000000
VERSION     DAYYFU010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.046"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHA34_001939"
     CDS             6..305
                     /locus_tag="ACHA34_001939"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8792557.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHA34_001940"
     CDS             378..1739
                     /locus_tag="ACHA34_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFM8792558.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHA34_001941"
     CDS             1761..2021
                     /locus_tag="ACHA34_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792559.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHA34_001942"
     CDS             2035..2787
                     /locus_tag="ACHA34_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFM8792560.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHA34_001943"
     CDS             complement(2881..3486)
                     /locus_tag="ACHA34_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8792561.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACHA34_001944"
     CDS             complement(3539..4513)
                     /locus_tag="ACHA34_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFM8792562.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACHA34_001945"
     CDS             complement(4891..5610)
                     /locus_tag="ACHA34_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8792563.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHA34_001946"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHA34_001946"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8792564.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACHA34_001947"
     CDS             7161..8558
                     /locus_tag="ACHA34_001947"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8792565.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACHA34_001948"
     CDS             8639..9079
                     /locus_tag="ACHA34_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8792566.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACHA34_001949"
     CDS             9227..9553
                     /locus_tag="ACHA34_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8792567.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACHA34_001950"
     CDS             9554..10303
                     /locus_tag="ACHA34_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFM8792568.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACHA34_001951"
     CDS             complement(10383..10838)
                     /locus_tag="ACHA34_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8792569.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACHA34_001952"
     CDS             complement(11265..11540)
                     /locus_tag="ACHA34_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792570.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="ACHA34_001953"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="ACHA34_001953"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFM8792571.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACHA34_001954"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACHA34_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8792572.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="ACHA34_001955"
     CDS             14770..17436
                     /locus_tag="ACHA34_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8792573.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="ACHA34_001956"
     CDS             17800..18552
                     /locus_tag="ACHA34_001956"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8792574.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="ACHA34_001957"
     CDS             complement(18574..19728)
                     /locus_tag="ACHA34_001957"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFM8792575.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT         6329 a         3472 c         4060 g         5960 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aatccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa atggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat t
//
LOCUS       DAYYFU010000046        19778 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000046 DAYYFU010000000
VERSION     DAYYFU010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.047"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..293
                     /locus_tag="ACHA34_001958"
     CDS             99..293
                     /locus_tag="ACHA34_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792576.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            562..861
                     /locus_tag="ACHA34_001959"
     CDS             562..861
                     /locus_tag="ACHA34_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792577.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(958..1392)
                     /locus_tag="ACHA34_001960"
     CDS             complement(958..1392)
                     /locus_tag="ACHA34_001960"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8792578.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1624..2334)
                     /locus_tag="ACHA34_001961"
     CDS             complement(1624..2334)
                     /locus_tag="ACHA34_001961"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8792579.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2324..3184)
                     /locus_tag="ACHA34_001962"
     CDS             complement(2324..3184)
                     /locus_tag="ACHA34_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFM8792580.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3189..3827)
                     /locus_tag="ACHA34_001963"
     CDS             complement(3189..3827)
                     /locus_tag="ACHA34_001963"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFM8792581.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3842..4141)
                     /locus_tag="ACHA34_001964"
     CDS             complement(3842..4141)
                     /locus_tag="ACHA34_001964"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8792582.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4184..5668)
                     /locus_tag="ACHA34_001965"
     CDS             complement(4184..5668)
                     /locus_tag="ACHA34_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8792583.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5688..6149)
                     /locus_tag="ACHA34_001966"
     CDS             complement(5688..6149)
                     /locus_tag="ACHA34_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8792584.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="ACHA34_001967"
     CDS             complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="ACHA34_001967"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFM8792585.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7452..8222
                     /locus_tag="ACHA34_001968"
     CDS             7452..8222
                     /locus_tag="ACHA34_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8792586.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8495..9322)
                     /locus_tag="ACHA34_001969"
     CDS             complement(8495..9322)
                     /locus_tag="ACHA34_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8792587.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9890..11125
                     /locus_tag="ACHA34_001970"
     CDS             9890..11125
                     /locus_tag="ACHA34_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8792588.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12124..12384)
                     /locus_tag="ACHA34_001971"
     CDS             complement(12124..12384)
                     /locus_tag="ACHA34_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8792589.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12411..13712)
                     /locus_tag="ACHA34_001972"
     CDS             complement(12411..13712)
                     /locus_tag="ACHA34_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8792590.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14004..14462)
                     /locus_tag="ACHA34_001973"
     CDS             complement(14004..14462)
                     /locus_tag="ACHA34_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8792591.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14475..15806)
                     /locus_tag="ACHA34_001974"
     CDS             complement(14475..15806)
                     /locus_tag="ACHA34_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFM8792592.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15817..17259)
                     /locus_tag="ACHA34_001975"
     CDS             complement(15817..17259)
                     /locus_tag="ACHA34_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8792593.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17246..18010)
                     /locus_tag="ACHA34_001976"
     CDS             complement(17246..18010)
                     /locus_tag="ACHA34_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFM8792594.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18114..18989)
                     /locus_tag="ACHA34_001977"
     CDS             complement(18114..18989)
                     /locus_tag="ACHA34_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8792595.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19244..19328)
                     /locus_tag="ACHA34_001978"
     tRNA            complement(19244..19328)
                     /locus_tag="ACHA34_001978"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19292..19294),aa:Leu,seq:caa)
     gene            complement(19346..19417)
                     /locus_tag="ACHA34_001979"
     tRNA            complement(19346..19417)
                     /locus_tag="ACHA34_001979"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19383..19385),aa:Gln,seq:ttg)
     gene            complement(19427..19499)
                     /locus_tag="ACHA34_001980"
     tRNA            complement(19427..19499)
                     /locus_tag="ACHA34_001980"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19464..19466),aa:His,seq:gtg)
     gene            complement(19561..19645)
                     /locus_tag="ACHA34_001981"
     tRNA            complement(19561..19645)
                     /locus_tag="ACHA34_001981"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19609..19611),aa:Leu,seq:caa)
     gene            complement(19666..19736)
                     /locus_tag="ACHA34_001982"
     tRNA            complement(19666..19736)
                     /locus_tag="ACHA34_001982"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19702..19704),aa:Cys,seq:gca)
BASE COUNT         6276 a         4090 c         3077 g         6335 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc
      541 agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag
      601 caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag
      661 tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata
      721 tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa
      781 aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag
      841 gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat
      901 cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta
      961 tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc
     1021 catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc
     1081 atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt
     1141 caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat
     1201 aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc
     1261 attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt
     1321 tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga
     1381 atcaatattc atttttttct tcctttctta tacaattctc cctctaataa tacagttctt
     1441 tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt
     1501 cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt
     1561 cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca
     1621 tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa
     1681 atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca
     1741 aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa
     1801 cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt
     1861 aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt
     1921 cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata
     1981 agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt
     2041 aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga
     2101 tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc
     2161 tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg
     2221 gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag
     2281 atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc
     2341 cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt
     2401 cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca
     2461 aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact
     2521 ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat
     2581 cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta
     2641 ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg
     2701 aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag
     2761 aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt
     2821 tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg
     2881 ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag
     2941 aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc
     3001 tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc
     3061 gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg
     3121 aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag
     3181 ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc
     3241 ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca
     3301 aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca
     3361 atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca
     3421 tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt
     3481 tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc
     3541 tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca
     3601 acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta
     3661 tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt
     3721 gtgcccactt caataatatc tactacatca ccaactttca taacatctcc taatgcatgt
     3781 tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc
     3841 tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg
     3901 acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat
     3961 tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg
     4021 aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca
     4081 cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca
     4141 tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt
     4201 ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt
     4261 gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca taacctagga
     4321 ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac
     4381 caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga
     4441 ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa
     4501 ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg
     4561 atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc
     4621 caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa
     4681 cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga
     4741 atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca
     4801 taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt
     4861 tgcctagtac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca
     4921 tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt
     4981 gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa
     5041 atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt
     5101 cctgcgtgat tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc
     5161 ccataaaaat caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa
     5221 aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg
     5281 tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca
     5341 tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc
     5401 caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga
     5461 ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg
     5521 ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta
     5581 ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg
     5641 tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct
     5701 gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca
     5761 tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt
     5821 gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa
     5881 cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt
     5941 tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct
     6001 acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca
     6061 agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc
     6121 aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg
     6181 gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact
     6241 caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt
     6301 taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat
     6361 cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg
     6421 actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt
     6481 caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat
     6541 tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt
     6601 ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt
     6661 cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga
     6721 ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt
     6781 gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa
     6841 ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt
     6901 ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca
     6961 tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat
     7021 gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt
     7081 tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt
     7141 ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact
     7201 tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt
     7261 ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg
     7321 tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag
     7381 atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg
     7441 aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg
     7501 aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat
     7561 ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca
     7621 attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg
     7681 aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc
     7741 tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt
     7801 tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt
     7861 atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat
     7921 taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg
     7981 tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca
     8041 acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa
     8101 agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg
     8161 aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt
     8221 aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg
     8281 ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa
     8341 tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat
     8401 caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg
     8461 gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct
     8521 ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta
     8581 aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca
     8641 atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg
     8701 ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga
     8761 ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt
     8821 tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta
     8881 agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg
     8941 catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta
     9001 aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt
     9061 ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg attttgtctt
     9121 cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa agcgcactta
     9181 agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt gcttgttcac
     9241 aaatgccttc tgctaattgt acttgtgtta accccttttt ctttcggtat tcttttagta
     9301 atgctccatc tactttaatc aaaacaattt ccttcccttt aaaaaattat ttctccttta
     9361 tatcatacac aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa taaaaatttt
     9421 aattttaaaa taatattcaa attaaacaac tggcgtatct gctccttgat ttctttttgt
     9481 gttatctgaa taattatcgt ttacagttat aacacatagt gaaaattgtc atggcgagaa
     9541 agtctaagaa tatttttaaa cataactatt tctttcttac gtgtgtataa tgacgacagc
     9601 acacctatca attaatataa ttattttatt tttttatgtt ctcatgttta tttttaatga
     9661 cacaacttct cgtatttatc tggcacaaca tataaatcga tcaaaaaatt gttgcgtgtt
     9721 tttttgaaaa ttggagtttc aacttgatta caaaatttaa atttagaagt tgtgaaaaca
     9781 gcgagagaca gtttaatatt caaaatatac ataggctact cgaacgctgt gttttttagt
     9841 atactagtaa gaaacgaagg gatagactca ttccaataag gaggatttca tgaagacaat
     9901 tctttttgct cctgcagtat tcaatttagc tgagacaaca cgaatgattg aggttgccaa
     9961 agctatgaaa gaccgttatt actgtgtatt ctttggcttt tctaaaactt ttgctccttt
    10021 gatcacagaa agtggctttg agtatcattt actagcgccg atccttacga aaaaacaaga
    10081 agaacaaatc ataaaatttg accagttgcg tacacttaaa aaccctttta ccaaagaaat
    10141 ggttgcctct cgtatcacta gtgaacagag tctgattgct aagtttgagc ctcgactgat
    10201 tgtgacaggt tcgaatgtga caattttttt atcggcccgc aataaaaaga ttcctttagt
    10261 atatgttaaa ccctatgctt tgagtgaacc tttttttcaa tcagagcatg cgctgatgcc
    10321 ccacgagtta cgtttcttcg gccctttaag caatcccctt tggcactgga caaaaaagcg
    10381 tatagtaaat acccgatgga tccccaatgc cttcaaacac gctgcacaaa aagaaatgct
    10441 tcctttacca aaaacaagtt tacaaatgat ggatgctgat ctaaatctca tcacaacgcc
    10501 taaactattt ttggaagaag aacagctgcc ggaaaattat cgaatcgttg gtccaatttt
    10561 tgctaagctc agtacttcct tgcctgaaga agtgctaact tttatacagc gaaagcaaca
    10621 agaaagaaaa cgtattatct atttcgcaat gggtagttca ggcaatccgc gtatgttacg
    10681 caaaattttg ttcttcttac aaaaacaacc ggatctagcg gttattgcac ctattgacta
    10741 tctgcttgat gacattcaat ctgactcaga agatctttta ataacagaat atctgtctag
    10801 tcatcttttg catgaattca ttgatttttc ttttatccac ggcggggaag ggaccgtcca
    10861 aactgcctgt gaatccggca aaccgtttat cggtgtgggg atgcaatttg agcaaagctg
    10921 taatattgac tattgtgtaa aatacggaaa cgcaatcgcc ctcacaaaac cagtgactga
    10981 aagaaaactg cagattgctc tgcgcgaagt ttcttcaccc aagattcgtc agcaggcacg
    11041 cacgctgcaa gccgtttttc caataaatgg tccacaacaa gcaattcaag aaatcgaaac
    11101 cttcctgagc aaccacccaa tatagtttca agtagaaaaa gcttttttaa tagagactca
    11161 tcagtaattt cgctattcga gacccagaca gtgaaaatac aaaaaaagta aatagtggat
    11221 tgaaaagtta gagttactta aattacggtg tctaaaaaac acaaaatcat ccttaaaata
    11281 aaagatctga cgctatccaa atgagcatag gtagcgtctg ccatttattc ttagtcaaat
    11341 tctttgacac ctttctctgg ttggatcatc tcaacgtttc ttcaaaagaa agaccataat
    11401 aattaggcaa atacattttc ttaaaatgtg tcatatagat ctagaagcat cgctttgtag
    11461 agatttgtgc cgctagtccc ctcaacaatt actaaatcaa atgtaggatt taccacttta
    11521 tctttgataa tcaacctccc tcttctgttg agacaatgat ctcgcaatcc gatgcatact
    11581 cctatataaa ctgccagcaa tccccacata ttgagtagat catgtcagaa tgttctatgt
    11641 cgtcagcaac gatacactaa aagtcacgtt caccatgact gattgctatt cccagcacaa
    11701 ttatttctat acacgtttca tgatttgaat aaacactcac atcatacata ttaccattca
    11761 tacaccatac tgcagcccct actatatggt tgtaagctcc caaatctata tttgacttga
    11821 tcactttggt agtagatcta ctaattctaa atccattgct gataaaattt ccattttttc
    11881 ctccttcttt ctgccaaaac agttaaaaca ataataatct ttatgtaaaa aagggctgtg
    11941 acaaaagcct tgtcacagtc ccatattccg aataaacggt ggtcaaaagc ttgcatctgc
    12001 ggtaataagt tcaaccaaac gaaaaataag ccaacatttc acaaaaattt aagaagcaat
    12061 tttcgtaaat ttcttcttat ttcttgaagc taaccacttc tgttacaacc acttttagaa
    12121 catttaaagc ttcgatgtga caaaccatct atgctgctcg atcataaaat tgatagcaaa
    12181 aattccacaa agtacatatg tcccccatga aaacaaaaca ttcatatgcg gtacaatgat
    12241 ctttggaaga atcacgatca aaaatgatgc cagtgcccaa atccatcgat tgatttgaat
    12301 atgatatgta gctagcatat tcaatataag gtgactagcc gttaataaaa agataaaaac
    12361 aaaaaataaa atcagccaat acattgtttc ccaccttcta aaaaccaaac ttagacagtc
    12421 tggacttcgt tttctccacg actttgttca tctgacagca tctttttatc ataagctttt
    12481 acaaatggat aatagataat aaatgctatc aatgtgttga tcatgatcaa gataacaaca
    12541 cgccagtcat ttcctgctga aagataaccg ccgattggag ctggtaatgt ccatgttggc
    12601 aaaacagtaa atctattgac catttctaat ttgactgcaa aataactgat aatagtcaaa
    12661 actaaaggaa ctaagttgaa cgggatgaac atgtaaggat tcaacatcat aggagctcca
    12721 aaaattatgg gctcattgat attgaagata ccaggaataa tagaaaaacg accaacctct
    12781 ttcaagaatt ttgatttaca gaagaatata aacaaaacag ataccacgat cgttgctcct
    12841 gcaccaccaa ttgttaccgt ccattgataa aactgttcag gagcgatata gggtaattgg
    12901 gtggcagctg taccatctgc caatgccttt gcattctcat ccaacatgat cagccacatt
    12961 ggacgaacaa cagcccctac aatactcatg ccatggatac caaatgacca caacaaatgg
    13021 atcaaaaaca ttggtagtaa aacaccaagc aaattattcc ctaaaatatt ggttaaaggc
    13081 tcgaataacg ataataaggc agcgtttaca tcaaagcccg cgacgcttcg aatcagccaa
    13141 gtaattgtta aaatgactgc acctggaatc aatgcctcaa aactacgggc aacagcaggt
    13201 ggcacttgtt ctggcatttt gatagtaata tttcgatttt taaagaaacg atatacttcc
    13261 actgcaaaaa tcatagataa gatccccgca aacatcccag atgctcccaa agaactcata
    13321 ggtaagaccc agccaagagg tgcctcttca ggagttaaaa cgtttacagg aacttgtaac
    13381 ataaagaacg ttcctaaaga taatatacca cctgtcacac tatctaatcc atatgattta
    13441 gctagatccg aacccataac aaaagttgca taaatagcca tcaatcccat cgtcatccta
    13501 aagggtaaaa caatcgttgc cgaataaggg gcaataaatt cattccatcc aggaaacggt
    13561 aaattgataa agaccataaa aaatgtccca atcaaaatca atgacaatgt tgaaacaacc
    13621 ccattacgaa ttgctaataa atgacgctgt gtaccaattt ttgccattgg tgggacaagt
    13681 ttctcttcga cccatttcat cagcttttcc actaaaacca ctccttttca ataaaatatg
    13741 aaccattcat tgcatgacaa cgcaaagaat gttcataact aattatataa ttaagaaatt
    13801 ttatttcaat atattctctt aaataatcaa atttattttt aagaatcctg aaaataagtt
    13861 ttctaaatca taggagcaac aaaaaaattc gataattaac aaaaatcaaa atgattaaat
    13921 ctagtgattg aaacatatca tttcaaaaaa attttgtaaa ttcacccata aatatcaccc
    13981 tatttaactt ttttcatcgt acactaaaat tcaaaacaac tatctttaat ctttacttct
    14041 tggttttgtt ttaataaatt tccttcaaaa atgcctggag aaactacaat gatcaccgta
    14101 tctttttgtg cttgctccaa cttcttcaaa tctataatag ctaacagtgt cccttctttt
    14161 acctcctggt tactttcaac ccgtatctga aacggacttc cttcgagttc aactgtatct
    14221 aaacccatat ggatcagtat ttttctgcca gattcacttt cgatcgttat ggcatgtcgg
    14281 gtaggaaata tattatcgat tattccttta actggcgcat ggatatatcc atcatgattc
    14341 gttatagcgt atccaactcc tatcattttt gaagaaaaga cctcgtcttt tactgctgtt
    14401 aatggaataa tttgaccact agctacagct tttagaatgt ttcttgtttt aaagaaatgc
    14461 actttttatc acctctactt taatgctggc caatattttt tgtttacttt gatcatttca
    14521 tctagtattt tttttgcgac tctggcattt ggtacggttt gattcaatgt aaatgcttgt
    14581 aaagcttttt gataagaacc ttcaaagtaa gcatctacta gtaacttctc tactgcaact
    14641 tgtgcctcca tcaatccttt atgaaaatca ctgattggtt ttcttagtgt aattggctcc
    14701 acaccagttg caccaacata tgccggtatt tcaacaactg catctgaccg tagattcgga
    14761 atcgcccctt gattaggaac gattagcata aagcgacgac gatcatcatt caacaaggaa
    14821 atagctatat cgacgatata ttgtccatgc tccccaaaat taaagttcaa tacttcttcc
    14881 acaataccgc ggcgtatttt atcagccatt tctttggtat ttttctctcg tccatccatg
    14941 accatattag cacgtgtata ctctttgtca gtatgctcaa cgaccatatc aggatataag
    15001 taatactcta agtaattatt cgggatatta cttggaaaat ttttcgtgat gactgacatc
    15061 atttgaaagg ttttttgcca agttttatct cctatattaa aatcggcgac ttgcatttct
    15121 tttgttatta gtttttcaat aatctctggc ataatttctc gttgcaattc tttatcataa
    15181 atcgatgtat accagccaaa gtggtttaaa ccataatacg taacgatcca attcttacga
    15241 tcataaccgt aattcactgc aatcgtttct tcaattgaaa tagtcatatc acaggcatta
    15301 atcattttta cattcggata tttacgacga acagcttcag aaacgatcga ctctggattt
    15361 gtataattca aaatccatgc tttgggggca tagtcttgta tatgatcgac aatttccaat
    15421 aaacctttga ttgagcgcat cccataagca aatcctccga gtccacacgt ttcttgtcct
    15481 actaatccat gtttaagtgg aatcttttcg tccttttctc gcatctccaa tcctccaaca
    15541 cgaatttgtg agaaaacaaa atcgcatcct gtaaaagcaa gctttggatc atccgttgca
    15601 cgaatgcgaa tatttgaaaa accttttctt ttcagcataa atttaacaat caagtacata
    15661 tccatatttc tttcttcatc aatatcatat aaacgtattt cagataatgg caaccgtttt
    15721 tcattgttca aaatggcttg aacgattcct ggtgtatacg tactcccgcc tccagctata
    15781 gtgataatct gttttttcat catcatcttt tcctttctat tcttctaata atttattgaa
    15841 ttctgccata acataagtca cttctaagcc gatgataatt tgataatttt tttcagttgg
    15901 tttaatgatc ccgcttatcc ctaattgctt gattcgctgt tcctcgacca tggtgggatc
    15961 cactacctct acccgtaggc gtgtattaca actatagaca tccaggatat tggcttttcc
    16021 tccaaacgct tgtagcattt ttgttgccat atattcataa tttttacttg ctaaccctaa
    16081 ctctgtttct tctaaggtta cttcattgcc aaactctgcc ttagcttcac gtcctaatgt
    16141 ctgaatgttt ctcttattga tcaaataata aaaagtaacg taatacaagg caaaaaagaa
    16201 aattccgatt ggaagaatca aaatagcatt ggtagcgatt cgaaaattga tgagataatc
    16261 aacaatacat gctccgaatg taaaaccaat tcgtatattg aataagtaac agacaatccc
    16321 tgctaaaccg gcaaaaagtg catggatcac gtacaacata ggagaaagaa acatgaaact
    16381 aaattcaaca ggctcagtga tatttgcaat aaatgaagtt cctgcaccgc ttcccatgat
    16441 tcctttgacc atctctttat tttctttata agctgcttta tagatagcta gacatatagc
    16501 tggtatacca aacatcatta ctacaaaaaa aactgataaa aacagtccag cctgaggatc
    16561 tccgttgata aagcgtgtca tctctcctct gaaaacttcc ttgctaggag atgtatagct
    16621 tcctaaatcg taataaatat acgtattcaa cacatggtgc agtccaaagg ggatcagcaa
    16681 acgattcaaa aacataaata tccccacacc taatgctccc atatcaacga gcagttttgc
    16741 aaaattatca atcccaattt ggatgagcgg ccagataaag cccaaaaaaa cagctgcgat
    16801 tgtttgtaga atcatcacca tagtcaatgg gaatttttcg cccgcaaaat aagaaaaaac
    16861 tactggtaat ttttgttctt taaagcgatt atacgtccat gccgctacta aaccggaaat
    16921 aattccacca aaaatcccca tattcacttc tgggtccata atttctaagc cttttttcaa
    16981 ggtagcaatc gctaaaaaac cagtaagtgc aggtattcct ttgtccttat ttttgacaaa
    17041 accgattgca actcctatag caaataagat atccaagttt ccaaaaacgg catttccagc
    17101 tgcaccaatt actgggatgt tcagcatatc gtcagctgat aaacgcatga tcaatcccgc
    17161 taatggcatc gctgcaactg gaagtagcat cgctcttcca agcttctgca catttgattt
    17221 aacattcatt tttattcctc ctctattaaa tatcctcata tgttactaac tcaatcaaag
    17281 gatcattttt cactttttga gcaaatgctg aatgaattaa aaattcgact tggtgggtac
    17341 gaggatacac aaaacttgaa ccatttaaca attcctgatc caaatacgct gcgtgacaca
    17401 tgacttcgat cgttttatct tctctcaata tcatgagtaa acttgccaaa tattcttcca
    17461 cactcgtatt gatttgtcgt tttttttcat cagtttctcc tacgacatcg aaacaaggct
    17521 caaaataatc gacatgacga acttcatctt ttcgttcaaa gttgctccga accggcaaat
    17581 catatttttt tgctaatgta acaactacct cctgattttc atccaaagta tgagtatgat
    17641 gatgactgtc taaatgagtt ggtatgatcc ccgcatgata aattttttga atctgagcat
    17701 tccattcctg atacagttga tctttgtcaa tgttaaatgg atgctgataa taagataagg
    17761 atctgaactc tccgctttcg ataagagtat cgacagtttt gaggagtggt ttgccacacg
    17821 tcaatgttaa atggactcct acacctagcc taggcatttc ctttcttagt ttcacagcat
    17881 gctcgaaacc cggcatattt gccatcaatg tagttgatgt caaaattcct cgctgataag
    17941 catccataat cccataattc acaccatggc tatagccaaa atcatccgaa ttaaaaatca
    18001 cttttcccat ttcattctcc ttcttttttc tactaaaata ggcgcaacca aataatcata
    18061 gtccatttac tatgtgtcat ttggttgcgc ctatagatag ttacgccctt attttaatag
    18121 attcttgcta gatgaattgc caaatagatg atttcatttt cttgaatggc aagttgaaat
    18181 tgctgttcga tgatatcttt aatctttagt gctgtacaat acgctttgct atgtttaaat
    18241 gtttcagtaa aaaaaccaat attttctact ggcgtttctt cctgataaat caagcgttta
    18301 accaggaatt tgatatgaac cattaagcga tgataagtca gtgattcttt atcataaacg
    18361 tttgaatagg atttttctag atgggacaaa atcgtggtcg tcatttctac tatttttaag
    18421 ctctttaaac tattaggctc tcctagctca gcattaatga tatgcatagt gatgaatcct
    18481 acttcatttg accctaactc ggtttgatat cgatgattga ttcgctcgac cacttctact
    18541 gctatatcat attcatttgg ataaaggaat ctcatttctg gcaacataat tgctgtgaca
    18601 tctctatctt ctctttttcg ttgaatagca aaataaatat gatctgtcaa tgtaatatag
    18661 atatttgaat ctaatggaga ggagatccta gctgttatca gttcaatcac ttcttccgca
    18721 aaatccataa attcgattgg aatctgcgag actatctcac tgaatcgttt gatcttttct
    18781 ttagaatcta atacaaataa tttgtcagca tcctcaacat caaatggctt actcacttta
    18841 gcagaaaagc caatcccttt ccccatcgca attttttcta ttccatcgtc atcgatcaat
    18901 acagcattat gattcagctt tttcatcaca tacaccgtta attcacctcc tctatatgat
    18961 aaaacaaaac aacttgcaga ctccttcata catatttttg tctccaaaca atttttaaag
    19021 acaaaaaacg acctaagaaa gaaccctttc ttaggtcgtg cctaattgaa tagtagcacc
    19081 ctacataatt tacaaaaata gtttaacaca taataaaagc gttatcaata tcttttttac
    19141 tcgttttatg ataatacttg tatcgaattg atttggaggt aattacataa aaacaaaaaa
    19201 aatatagcaa ctctctataa gaaagttgct atattgataa ttataccggc ggccggggtc
    19261 gaaccggcac gtcctcaacg gacactggat tttgagtcca gcgcgtctgc caattccgcc
    19321 acgccggcaa atttaaaaat aataactggg gtagctggat tcgaaccaac gcatgaggga
    19381 gtcaaagtcc cttgccttac cgcttggcta taccccaaga ataaagggcg aatgatggga
    19441 atcgaaccca cgaatgccgg agccacaatc cggtgcgtta accacttcgc cacattcgcc
    19501 atcataaaag atgttattta atttctcaat aaaaaactaa gatgaagaca tcttagtcaa
    19561 taccggcggc cggggtcgaa ccggcacgcc ctcaacgggc actggatttt gagtccagcg
    19621 cgtctgccaa ttccgccacg ccggcaagtt gatttattag tactaaggcg gtaaccggat
    19681 ttgaaccggt gatgaaggtt ttgcagacct ctgccttacc acttggctat accgccatta
    19741 tactattatc aatctataaa ataaagaata aattatag
//
LOCUS       DAYYFU010000047        19476 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000047 DAYYFU010000000
VERSION     DAYYFU010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.048"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..996)
                     /locus_tag="ACHA34_001983"
     CDS             complement(22..996)
                     /locus_tag="ACHA34_001983"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8792596.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1076..1951)
                     /locus_tag="ACHA34_001984"
     CDS             complement(1076..1951)
                     /locus_tag="ACHA34_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFM8792597.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2081..3988
                     /locus_tag="ACHA34_001985"
     CDS             2081..3988
                     /locus_tag="ACHA34_001985"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFM8792598.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4529..7807)
                     /locus_tag="ACHA34_001986"
     CDS             complement(4529..7807)
                     /locus_tag="ACHA34_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFM8792599.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8078..8623)
                     /locus_tag="ACHA34_001987"
     CDS             complement(8078..8623)
                     /locus_tag="ACHA34_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8792600.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(8635..9327)
                     /locus_tag="ACHA34_001988"
     CDS             complement(8635..9327)
                     /locus_tag="ACHA34_001988"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFM8792601.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(9346..9630)
                     /gene="macP"
                     /locus_tag="ACHA34_001989"
     CDS             complement(9346..9630)
                     /gene="macP"
                     /locus_tag="ACHA34_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFM8792602.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(9643..10206)
                     /locus_tag="ACHA34_001990"
     CDS             complement(9643..10206)
                     /locus_tag="ACHA34_001990"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8792603.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            10434..11096
                     /locus_tag="ACHA34_001991"
     CDS             10434..11096
                     /locus_tag="ACHA34_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFM8792604.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            11220..12416
                     /locus_tag="ACHA34_001992"
     CDS             11220..12416
                     /locus_tag="ACHA34_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFM8792605.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(12517..13893)
                     /locus_tag="ACHA34_001993"
     CDS             complement(12517..13893)
                     /locus_tag="ACHA34_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8792606.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(13930..14796)
                     /locus_tag="ACHA34_001994"
     CDS             complement(13930..14796)
                     /locus_tag="ACHA34_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM8792607.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(14885..15910)
                     /locus_tag="ACHA34_001995"
     CDS             complement(14885..15910)
                     /locus_tag="ACHA34_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8792608.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(15928..16602)
                     /locus_tag="ACHA34_001996"
     CDS             complement(15928..16602)
                     /locus_tag="ACHA34_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8792609.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(16786..17679)
                     /locus_tag="ACHA34_001997"
     CDS             complement(16786..17679)
                     /locus_tag="ACHA34_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8792610.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(17753..19450)
                     /locus_tag="ACHA34_001998"
     CDS             complement(17753..19450)
                     /locus_tag="ACHA34_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8792611.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLAKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         5683 a         4189 c         3446 g         6158 t
ORIGIN      
        1 ttctttccct cttttttttg tttattcgaa ttctttttct tccagacgga ataacggact
       61 catcggtgta ttttcatgga tgcgtttgac cgcttcaccc attaattctg aacatgtaac
      121 gattgttaag ttttctggat gacgattttc ttctgtgaaa acagaatcag tgatacaaat
      181 atctttgatt ggtgcattgt ctaaaatact tttcgcatga tcggacaata atccatgtga
      241 cgcacaggca tatacttctt ttgcgccgtt ttcttttaat acatttgctg ctcgtgcaag
      301 cgttgctcct gtgttcaaga tatcatctac catgatacat gtctttcctt gaacatcacc
      361 gatcacataa ccaccctcag ttgaagcatc atctgcatga tctacgatgg ctaatgtgct
      421 gttcaaatat tctgaaagac tacgtgcacg ctgaacaccg ctgttttttg gcgaaacaac
      481 tacgatgtca tctcccgtca gacctaattg acggtaataa tgagcaaaca acggcatcgt
      541 aaataaatta tctacaggaa tatcaaagaa tccttgtact tgaaccgtgt gcagatctaa
      601 agtcagtact cgagttgccc ctgcttcttc gatcaagtta gcgatcaatt tcgcagtgat
      661 cggttcatga ggtttcgccg tgcggtcttg cctagcatac ccatagtaag gcaatacgac
      721 attgatcgtt ttcgcacttg ctcgcttcat tgcatcgatc atgatcagta gttccatata
      781 gtagtcattg acaggtgcat tcgttgattg aacgatatac acatgatccc ctcgaatact
      841 ttcttcgata ttgatggaaa tctcgccatc actaaattgc ttcacgacgc ttttacctaa
      901 ttctgttccg aacactttcg cgattttttc agctaatgga cggttgccat tcaaactaaa
      961 aatcttcaac gtgtcatcct gatacttatt actcatgtag tcctccccaa aacttctcta
     1021 ttcttcgata gtttaacata tttttccaag attttctgtt aatttcagca aattcttaag
     1081 gaagtgaaac tgttccgtta ctccaactgg caggaatgac tttatgataa tcgccccagt
     1141 tgtcgtgtac tttgtaataa cgttctcctg tttcagtttc aaaataagcg tacgctgtca
     1201 cccaatgatt gccatatgta ctgccaagta ctttcaaaat accaatcatc acaggtttgc
     1261 cttcacgtat acgttttgta gctcgctgcc atgaacctag aagagttgct cgtgcacgct
     1321 catgattgcc attcttcttt aaaaaactag aaatccctgt actaacctga aaaggaatcg
     1381 taggaagtcc tagtggttgg atctggctac gcaatgcctt cgtcaaaacc aacgaatcag
     1441 ccgtattttt cttgcgaatc tcatttggaa gcatccaccc cttacgatag tcttgataat
     1501 aggcaaggag aactgctgcc gcgtaagtac cacaaagata gcctgagggg gtagcccatt
     1561 tcttatacct caaaaaacgt tcttctggca atcccaccca gcaatttgcg agttcctttc
     1621 ctgacctttt ttcttcgatc aaaacgggaa tatcttcttt tctgttgatc catctagata
     1681 actcgattgg ctcttcattg atcagcttcc attttttaca agaaagaccc tcttgataag
     1741 tatattttgc tggagaatat tctaccggat gtcctgattg atcgataatc acataacttt
     1801 gatcttcaaa aaccagagct aaaaaaagca atttactttt tcttgactgc agctgctgag
     1861 tgctggctaa ttttttaatt tttttatcta ctttgaaata taaaggttta tccactactt
     1921 gattcagttg ggatcgcctc ctttttttca ttataagtga aggattgaaa aaaacaaatc
     1981 agtttgattg ttcgaatttt caatttttta tgttctattt gttaattcct tcattttgta
     2041 agataatggg cacacgaaac aaaaaaggag cgatcgatga atgaacaaag aacgattgaa
     2101 acaagatttc tatgaagcag tcaatgaaga atggcaaaaa acagcaaaaa tccctgcaga
     2161 taaaccagct acaggcggtt tccaagattt agttgacggg atcgaccaac ttttgatggg
     2221 agaaacagac gatctgcttg cccatccaga aaaagtgtct tcagaaaaaa tgaaacattt
     2281 ccttgcatat tatcaactgg caaacgatta tgaccgaaga aatgagttgc atgctcaacc
     2341 gcttcaacct ataatcgaaa aagttgaatc attgcagtct ttcgacgaat tgaataagca
     2401 atttccagaa tggctgttgg atacattacc attgccattt aatctggatg tagatgcaga
     2461 tatgaagaat gcgcaaacga acgccttctt tgcttatccg ccttctttat tccttccaga
     2521 taagacgtat tatgcatcag accatcccaa cggtgcacaa ctaataggcg ttttctttga
     2581 tatggctgtt cagttattag agcttgccgg caaagaaaaa agtcaggcag aagcaatcac
     2641 ggaacaagca gtgcaatttg ataaattgat tgctcctcat gtaaaaagtg cagaagaaaa
     2701 cgcagattac agcaaaatgt ataatccgca atcttttgat acatttatta aatacacaga
     2761 taaactggac ttgaaaaaac ttgtcacggg tttgattggg acaactccag ataaagtgat
     2821 cgtaactgat ccagtgtact tcgatcattt gaatgacatc ctgacagaag ctcattttcc
     2881 gttattcaaa agctggatga tcattaaaac agtacgttct cttagtggtt atctatcaga
     2941 agaattccgc caagtcagcg gaatctattc tcgcgtattg tctggaacag acgaagcaat
     3001 gcctcagaaa aaagcagcgt attaccttgc ctctggacaa ttcgatcaag tagttggcga
     3061 ttactacgga aaaaaatatt ttggcgaagc tgctaaaaaa gacgtagaac aaatggtgaa
     3121 aaaaatgatc gccgtttata aaaaacgtct agaagaaaat gactggcttg gggatgcaac
     3181 aaaagaaaaa gccgtcataa aattaaataa cttaggtatc caagtaggct accccgataa
     3241 gatcccagca ttattcgacc agttcaaaac agtacctgca gatcagggtg gtactttgct
     3301 ttcaaatact cttgttttca gcaaactcac attaaaagat cgtttcagca aatggaacaa
     3361 accagtggat cgttcagaat gggaaatgag tgctgacacg gtcaatgctt attatcatcc
     3421 atttcggaat gtgatcgtct ttccggcagc gatccttcaa gcaccatttt acagtctaga
     3481 acaatcaagc tctgctaatt atggtggaat cggagctgtt atcgctcatg agatttccca
     3541 tgcatttgat aataacggcg ctttatttga cgaatatggc aacttgaaca actggtggac
     3601 agaagaagat cttacacaat ttcaaaacaa agcacaagca atgatcgaag aattcgatgg
     3661 tctgcctttt gctgacggaa aagtaaacgg aaaattaacc gtttcggaaa atattgctga
     3721 tgcaggcggt ctaagctgtg cattggaagc agcaaaaacg gaatcagatc tttctatcga
     3781 ggaattcttt gtcaattggg caacgatttg gcgtacaaaa gcaaaaaaag aataccaaca
     3841 acttttactg caaatcgatg tccatgcccc tgctaaactc cgagcaaacg tgcaaccaaa
     3901 aaaccttgac gaattctatg aaacatttgg tatcacggaa caagacgcta tgtatctagc
     3961 gccagaaaaa agagtgcgaa tctggtaaga ggttgtgcca gaagtggtca gcttcaagaa
     4021 ataagaagga atttgggaaa atcgcttctc aaatttttgt gacatttcgg cttatttttc
     4081 gaagaagtta cttctggaat accatgtatt agaggttgtg aaaaagctgc ttagtttcaa
     4141 gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt tgacttacta
     4201 ccgagaaact ggcttttgaa caccgtttat cgagaggttg tgaaatcagc gtcctgacct
     4261 gagtattaga gaaaaaaacg gaaaaatagt tcctcatatt ttgccgtttg ttgagctaat
     4321 accgagggtc agctgattga acaccgttta gtggagagat tgtgaaaaag ctgtcctgca
     4381 tcaagtaata agaacagcca atcaaaagca gcaaggagaa cctcatctat aaaaagatag
     4441 aaggaagtga gacatctatt ggtctcattt ccttctattt ttactttcct cagtcaattg
     4501 ttttacatca taaaaggaga aattccactt atttttttcg ctgtttcatg acataaacaa
     4561 ctacaccgat taacaaaatt cctccagcta atgctagcaa cgggttatta aattctcccg
     4621 tttttgggag tccttgagtg ccattttttt cactttcttt tgctgtccta tccggatgag
     4681 aaggttttcc atttggtttt ccatttggtg attcatttcc tggattttct ctttctggtt
     4741 tgtcatttcc cgggggcaca tcggcagttt ctcgcacagt gatcgctttt tgctgggtaa
     4801 cagattctgt atgagcagac catttttccc catcaaatat ttccgaacga taagtgactt
     4861 ctagcgtata ttctcctttt gaaggtgctg aaaaaatagc ggtatgatta gaaaattgag
     4921 cacttgactg tgtttcaacg atttggatct tttctgggaa ataacgcata tcttcaaaga
     4981 caggcgttcc ttgatttggt gtgaaagttg acacaaaaac tgtaaaaggc ttcagttcaa
     5041 tgatatttcc ttctgcattc tccgccacac catttgcttc ttgtataata atcggattat
     5101 tttgttcatt tggttttgtc tgatttggtt cttgtacgat tgcatactct ggatattccg
     5161 aagacggtga ggtttctccg tcaaaaatag cacctacaga taatggaaga ccatccactc
     5221 tttccttttc tgtttgacga ttccccggtt taatctgcat cagtccatga acatggatac
     5281 ttccattttc atagcgcgta acaggctgct gaccttcttt cgaacatgta tttagagact
     5341 cgatccaatc ttttgatatg gtatgtccaa gagtattttc tgattgtttc tctcgttcat
     5401 tccaatagta gttcaacgag tggtagattt gatcttcgtt ttgattcaat gggcggatct
     5461 gctctctcat ctcaagattc tctgttcgga aagataagat accagaagca tggtagtctg
     5521 tgtttcctgc tgaagaggta taaagagcca cattatatga accgttatta aaacttgtac
     5581 tgctattgac gataatatct gttccgctat tggaatcgat tcctttcgca agattttcga
     5641 aagacaaact attgatcaat tcatgagggc ccgttaaaga agaaccgccc attttgaatc
     5701 cattaccgtt accttctccc tcaattcctg gtacccagcc gtttcgataa gcaactgagt
     5761 ttttgatgat tacttttccg atcgaacctg tttcattttt agcaaaaagg tcccaaccgt
     5821 catctgcatt atgatacgcg atacaaccgt caaatacatt tccttctcct attgtcagtt
     5881 tggccgcaaa tccatcggca tcttcaaatc ctggatcggc attttcaaaa gatgtacagt
     5941 tcttgactaa gttatgcgcg ggccatgctt cgaaaggatc atttgagcta ccgctgattt
     6001 ggattcctgt atttccattc cgataagcat gaatctcttc gatcaaatta tgatttccag
     6061 aaatctgtaa tcctttttgc atagctgcac tatttgtaat gtcaaaccct cgtaaacccc
     6121 agtagtgact gaacagtgtc atcccatttc cagttccttt aaaatcaagt acaggacgct
     6181 tgttttctgg atttttctct gcaatcaact gaatcgggtt ttctcttgtc ccgttaacgc
     6241 cttttggaat cgtcaatcct ctttcgaagg aataagttcc aggcgccatg acgattgtct
     6301 gcccagcata tgcgtatctc aatgcttgtg tcagagataa tggattttgt ctgcttccgt
     6361 ttcctgcatc tgacccttct ggtgttacat aaactgtcga atatttaggt ttacggtagt
     6421 ccactgtatg aagaatctct tttgtttcat aactagtcat ctctacgtat tcttctggcg
     6481 gatagttttt atctggcgta aaactgaaag taaattcgtt cgttccttcc atcagttttg
     6541 tctgataatc aaattcccga ttcgcagtga tagccaagtt ttttgcgttc ttaagcacct
     6601 ctcctgttgc tttattttta attgtcaata atccgtcagc atttgctcta aaggacatag
     6661 tatgatcttg gtttcctgaa tgattactag aggtcacagt gtaaatcgga tcaatcaagc
     6721 gagtaggtct ttcttgccca ggaggatctg tcgcaggatc tgagtaagca acttcgattc
     6781 cttctacctt gatcgtcata ttccttgctg cagcaaatcc tgcataaata accgattcat
     6841 ctgaagcgta taatttttcc cagtcataca tgatgtcaga agccatttct tctcctgttt
     6901 caggatcgta ataatatgac tctaaaccag tattcgtttt tttcatctct acgtccagtt
     6961 tagtaatcga gttcgtggcc ggaaacaaat tcccatgacc gtttccgaat atattataag
     7021 aacctgtttc tttttccaag aaccctgctg aagtctctaa aggtgttgtt tccactgaga
     7081 cactcccagg agctggcgca gcttgcggat cttcagatga tattcctgtg attgcccgtg
     7141 tccccaatcc aagtcgcatc gtatatttgc tgccgctcgt gttgaccact tcttgtctat
     7201 ccgagtccca gacatattcg atccgactgc ctagtatggc aaatgaattt gaataaaatg
     7261 cctgcccaaa caaatgattt tttggtactg cgtctcttac cattaaagca aatccttctt
     7321 gggcattcgt ataggtccaa gattctattt cgatcgtcgc ccgcatccta aagttgatgt
     7381 ctttggggat tgcttgataa taataggtca ttccatctga tccgcttgac tggaattttc
     7441 cgccgttatt cgtggaagag agccgcacgc cgtcttctaa tggttcaatg gcatacctac
     7501 tagcacttgt gctttctccg aaaacaaccc ctttccactc ttcatttgct tcttcagatt
     7561 gcttttttgt cttttcattt tctgattctt tcatcatagg atcatttcct tcttccgtat
     7621 ccatatcttc actagttctt ggtacttcct ccattgttga catcccttca atgttcacgt
     7681 gtgcattttc ttcgttcaca gactgttgtt cgttagcagc aacttcaacg acagaaatta
     7741 aaaccggggt actgagcata aggagattca tcattagcgc actgtatttt atacgttttt
     7801 ttaacaagtg tattcctccc gtaacaaatt agaaagcgtt tacttaaagt attacctaat
     7861 ttatttttac tgtcaataaa aaacaaaatt ataataagtg tattttataa aaagaaaaaa
     7921 tataacttaa gttgttgata ataaagagat ttattaacgt aaaaagaata agttttttta
     7981 aaataacgta taaaaaagag cataaaacaa agtcgtttgt tttatgctct tcttttatta
     8041 ttttgtgctt tttattttat caaatcactt tgttttttta taaaatttct gcgcctagtg
     8101 tggactcaaa atgtctcaat gcccattcat gtccaacggg atcgaaactt gccacggcat
     8161 ctttgaaaat atggagtttg tatcctagat tatacgcatc gactgctgta tgcaacacac
     8221 agatatctgt acatacgccg gtcaaataaa tatctgtgat ctggcgttct cgcaaacgga
     8281 tgtctagatc tgttccacta aaagcggaat aatgccgttt gtctatccaa taaacatttt
     8341 cctcttgctc atagtcttgg tataaacttt ttaaagagcc gtacaattcc cgtccacccg
     8401 ttcctatcac attatgcgga ggaaataagc ggttctccgg atgaaaggaa tcttttggat
     8461 catgcgcatc aatggcaaaa acgacaaaat ccttttgatc aataaacttc tttgtatgag
     8521 cagtcagctt tttttcaatt acttgtcctg ctgtcccagt agtcagcttt ccatcctctg
     8581 caacaaaatc atatgtgtag tcaatcgaga tcaatgcgtt catcttagtc ctccttatac
     8641 gagatgtttg acaaattcga tcaccatttc tgctgatcgt ttgcctgcgt cttcgataaa
     8701 ttcgtcaaag ctttgtgtcg ccgagtgatc tgctgtgtcg ctcattgcac gtacgatcaa
     8761 aaatgggata ttgaactgac gagctgtttg tccaacagcg gctccttcca tttcgcaagc
     8821 taatgcttca gggaaattcg ttaagatttc tttgatttta tctggcgagt cgacaaatgt
     8881 atcacctgta acgatcaatc cttcttttgc gttcaaattc gttgcttttg ctgctttaac
     8941 catttcactg cgcaaatatg tgcttgcttc ataatataga ggcattcctg gtaattgtcc
     9001 tggtttgtag ccaaatcctg ttgcatctgc atcaaaataa gctactttgt ctgatataac
     9061 gatgtcacct acttgtaagc cttcaccgat tccgccagct gaacctgtat tgatcaccat
     9121 gttcacgccg tattgttgga tcaacagact tgtggtaata gatgctaaga cttttcctat
     9181 accggaacgt acgacgatca cttcgtggtt acctaatgaa cctgaaataa ataaagcgcc
     9241 tgcgcgttcc catgataatg gttcgcttag gttctctctc aaaattttta cttcttcttc
     9301 cattgcgccg ataataccga ttttcatctt tttaattcct tctttctata taaatgcgat
     9361 tgctaaaaaa accacacata acattaggat aacaataata agagatttca tcaaaaaaga
     9421 gttccatttg gtcgtctttt cccgttcgct gatccttgtt ttcgtgattg gtttattctt
     9481 tttgctttct ttacgataaa agctggcaat ctttttttct tcttgctgat aagctgtttc
     9541 tgcttttcgc tgttttttta atgactcgct tgcttgtgct tgttgtcgct tgcgaagttc
     9601 gcttcgcgta ataagcggtc ctttggccat aagccgatcc ctctactttc ctttggtcaa
     9661 tagttcccag tattgaatcg cataaatcgt ctttgcatca caaatcaact gatctttcat
     9721 cgcttgttgc gcctcttcaa gagttagatg atacaactcc agtacttcgt cttcatcttg
     9781 tgctaaagga ttctcgacct tctctacccc ttgtgcatga taaatatgaa ggacttcatt
     9841 tgcaaaacct ggtgaaagat acatcgaagt aagatggctc agcgacttcg cacgatagcc
     9901 agtttcttct tctaattctc tagctgctgt catttcggga ttttgtcctt ctcctggatc
     9961 gatttttcca gctggaatct ccaaaatcac tttttctaaa ggttttcgaa attgtttgac
    10021 aagaagcaga cgatcttgtt cgtcaaaagc aatgatcccg acaccaccag gatgaaatac
    10081 cagttctctc ttcgaagttc ctccatctgg caaacgaact tcgtctacag caacatcaat
    10141 aatttttccg ctatagattt ctttgcgact gattgttttt tcttcaaatt gctcattgtt
    10201 cagcatatct tctcctgctt tctttgtaat cataactgct tttttttcat ttttcaattt
    10261 ttctcaaaaa ctccttatta gtttaccata gataaacctt agattttctc taaatataga
    10321 aaatccttct ttagtccgat actctttcta gtagtccgta tgctaaaata agtatgtaat
    10381 cgaataacga aaagaattca atactcaacg ataaatgaca tggagggttt attatgataa
    10441 aacgaaattg gccgtggctt ctagcattgg tcttgctatt tctctttgtc cgaaacaata
    10501 gtttttcttt tgttctagga cttttattga taggtgtagt tttagttttg tggggactct
    10561 ttggttcgcg caggaaaaag ggaaaacaag aagaaattcc tgcactgact gacgaacttg
    10621 aaactcacta tgaaaaaagc ggtatgacag cgaatgaaat ttcatttttt cgacaaacaa
    10681 tgaatcaaac aaaaaatgag atcaatcaat tgcaaatgaa tatgcagcag acagccaaac
    10741 ttaaagcaat cgatctccgc catgatccag tcaaagcagc gaaagcttta ttcaaagaat
    10801 tggtgaaaga accaaaccga ctgcatgaag caagtcagtt tctttatacg catctgccga
    10861 atctagttga tttgacaaat aagtataatg agatcaatga ccatgagatc aaaaacaaac
    10921 agacctatga aaaactagaa gaaagcgcgc agatcatcga ccagctgtca gctttaatcg
    10981 cacaagatta tcaaaaattc gttgctgagg atctagatga tatcgacgta gagatatctg
    11041 ttgcgaaaca gagcttaaag cgtgataata aatatgaacc agaacaaaag gattaactct
    11101 tttgttcata aaagaagact cggaacattc agatgatgtc tggctgtttc gagtactttt
    11161 tctaaagtag agacattcat gtaagaatcg agaacagatt caacaaggag gaatttttta
    11221 tggatcataa accgactgaa acaaaagatg ttacaccagt taacgataca cttgaagatt
    11281 tattgaacaa cccgttttca actccaacag atacgctgac cacttcacaa caagcggaga
    11341 ttgatcagct ccaagagcaa cagacagctg ctcgtttaat tgacaaacta ccggcggaac
    11401 gccaagaaca agctaaacag ctagcagcaa aaatcgatgc taatgatgca cagtctgtca
    11461 tcagctacgg atcggctgcc caagcaaaat taagtgaatt ctcacaatcg atgttgaatc
    11521 atgtgcaagc acaagatatt ggtcctgtag gagattcctt aactgaactg atgtatcgtc
    11581 tccaagaagc caatcctgac gaactgcgtg ccggagaagg gaacatcttc cagcgtgtct
    11641 tcgggaaggt caaacaatcg atctatgaag tgacagcaaa atatcaaaag attggtgcac
    11701 aaattgacaa gatttcagtg aaattagata aagaaaaaga cggtctgcta aaagataatc
    11761 tgatgttaga acagctttac cagaaaaaca aagactactt tgatgcactg aatatttata
    11821 tcgctgctgg agaattgaaa atggaagaac ttcaaacaac gatcattcca gaagcgatga
    11881 aaagagcaga agaaactggc gatcagatgg atgttcagat tgccaatgat tacacgcaat
    11941 tcctagatcg tctggataaa cgtacgcatg atcttcgctt agctagacag atcacgatcc
    12001 agcaagcacc acaaatccgt ttgatccaaa acacaaatca ggccttggct gaaaagatcc
    12061 aagcatctat cgctacagcc attccgctgt ggaaaaacca agtcgtgatc gcattgacct
    12121 tgcttcgtca aaaagatgca gtaactgctc aacgccaagt ctctgaaaca acaaacgact
    12181 tattgaagaa aaattcagaa atgctgaaaa tctcagccat cgaaacagcg aaagaaaatg
    12241 aacgtggaat cgtggatatc gagacccttc aaaccactca aaatgatttg atcgaaacaa
    12301 tccaagagac attgcgtatc caaaaagaag gaaaagaaaa acgtcgtcat gcagaagtag
    12361 agcttggaca catggaagaa gatctgaaac aaaaactgtt ggatttaacc caatagatca
    12421 cctttcgctt gttactaaaa caaacgccca acaacagttt attacaactg ttgatgggcg
    12481 tttttcttat ctttcttcta acttctccat tggagtctat ttctcattca tcttgctgtg
    12541 tccatagcca tagccaaaat aaatgatcag tccaataacc attacgatac cgaacacgat
    12601 ccatgtcaca gtttgcagac gaagcatcaa tacaatacaa gtcaagatgg agacgattgg
    12661 caatagcgga acgaatggca ctttgaattc tccagctttt ggtttgccaa acatttttct
    12721 caacttcagt aagcctagac taacaagcat caaataggcg agcgccatga tattcgttag
    12781 ttccgctaat acattcaatg ggaaaaatcc ggcaaagaat acagctgcca caccagcggc
    12841 ataagtcgct tttttcggtg tacgatgctt ttgatcgatt tctgtcataa acttaggaag
    12901 taagccatcc ttgctgacag caaacagtaa acgagcaagt gaatacatca tcgagatcgt
    12961 taccgtcagt agcgttaaaa ttgcccctac agcgatcacc gcgcccactg ttccatgtcc
    13021 tacatacctc attgcaaaag ccactgggtc attgactcct agttcagtat acggaacgat
    13081 tcctgtcaag atcagcgtca cgatgatgta aaggacagta gcaatcagta tcgaaccaat
    13141 gattcctctt ggcacatctt tttgcgggtt tttgacttcc tcggctgcca ttgcaacggc
    13201 atcaaatccc aagaaagcaa aaaacacaag agctgcacca tctagcacac cggaaaaacc
    13261 aaaaggcatg aatggctgcc agttatcagg tttaacgaaa aatattccta caatgataaa
    13321 caacgcgatg ataccaaatt tgacatatac catggcatta ttcaaacgta atgctttctt
    13381 ggcttcaagc gacacgatgt aagtgacgaa tatcactacg agcaccgcaa tcaagtcaat
    13441 gtatgtacca ttttccggat tgtacccgcc agttaatgct tgtggaagtt cggcccctaa
    13501 acttttcaaa aaaccttgca tatagccaga ccatccggaa gcaacagagg aaaccgctag
    13561 taaaaattcc ccgatcaaca accaccctgt cagccaggca gcaaattcac caaatacaac
    13621 atacagatag gcatatggcc caccaatcac tggcactcgc gaagcaaatt ctgcgaaact
    13681 tagtccagac aaaatgacaa cgattgcagc taagacaaat gaaagtgaca gtgctggacc
    13741 agcattttga ttggccgcaa cccctgtcac cacaaatatt cccgtaccga tgatcgcacc
    13801 aattccaagc atgatcaaat cagacgtttt tagttctttt ttcatctcac tcgtatgatt
    13861 gagattgatc tctttcttac gaaacaaatc cattgaagtt cctccccatt ttaggttgga
    13921 aaacagcgat taaactgttt tcatataccc taaaaggata ccacctaaaa tgactaaaag
    13981 gcaaccaaca ataacataaa tgagctcttt tttcgttttt ctttcgccaa ggatgaaaat
    14041 cccacctaaa gtagagataa tgatccccat ttgtgaaagt gagaagccaa cagctaaacc
    14101 aagtgatttg actgttagaa gcatgcagat gtttcctaag ccccataata atccacagat
    14161 catgtttttc cacacaaact gatcgacttt aactttacgg aaagcaaaga agcttgcgcc
    14221 taagatcatt ccgatacttt gaggtaagat aattgacatc gcatccaaat tcgcccagtt
    14281 gacgatgatg gtatatacac cataaccaat cgtagagaag atcaatgctc taaaaccctt
    14341 accaaaatca agcattttat tatctgtatc tatttttcct tcgtcatctt gtcgggcagt
    14401 caaatacgca ccgcttacaa gaagagctaa agcaataata ccgtatacat aatctttcgt
    14461 ttgtgtccat tcatggaaga aaacggctcc cgcaatcgta ttcaaaatta attgaaaacc
    14521 agtagacaat ggtagcccta cagaaacacc catgtacttc attccatgga actgtccatt
    14581 ttgtccaaca ctccaagcta agccggataa aaatccgatc aagaaaatcc aaggggtgat
    14641 tacaggacta acaacgaaaa agacaactaa agaaaacaga aatgcaccga tcgtcattcc
    14701 tagtgtttgt tgattggcat cgccaccaat tttaccactt actaaaccaa tactacccca
    14761 tgccaacata gggactaatg ctattaaaat ttccataacc aatctccttt tatttcacat
    14821 gataagaaac ttatcagcga aaacgttaac aacacaagca ctttttttcg tttctaacga
    14881 ttttctaacg tttttcatta taaaaattta agattgggaa aaataaagtc attgttgtcc
    14941 cttttccttc agttgattca gccgtcaaat ggatacctaa tttttcggcc agattttttg
    15001 ctaaataaag tcctaatcct gttgaatgtg ttttgctaag tcgaccattt ctcccagtaa
    15061 atcccttatc aaagatccgt ttcaagtctt ccgctgggat accgatccct gaatctttaa
    15121 ctgaaaggct gactccttgc gcttcctttt ctatgctcac aatgatgttt ccgtgatccg
    15181 gcgtatattt gatggcatta ctaagcaatt ggttgaagat aaaagcgatc catttagcat
    15241 cggtcaaaac agtatgatcc tcaccaacga tctcgtagcg aatatttttt tggataaaat
    15301 aatttgcctg actgcgtatc acaggctgaa tgattccttt tagtccatat tcttggatga
    15361 ggtagtcccg cgagaaactg tcgagtcttg cataatacaa aacttgttct acgtattcat
    15421 cgatttttga taattcattt tctagtaatg tatacttttg atcgtcaata tcgaactcga
    15481 tcgaacgaac tagcaattta gaagcggaaa gtggcacttt gatttcatgg acccatgagt
    15541 caatataatc tttttggtct tgttgattac tgattgctga ttgcattact tcttggtgtt
    15601 cacggatcag ctgattgata tattcttcta ccagtttttc ttcttctgtc ctcccaccac
    15661 tcaaataatt ttgcaaaggg gaagtttttt gtatgtcggc taattttccc caccatcttc
    15721 tcttgctcaa gtaaccaccc agcagataaa aaatcagaaa gacgccctca atcaaagcta
    15781 aataagcaat tgtgctcaaa tcaactttga tgtttggcgc cagccacatc acaaagatcg
    15841 tcaatgcaat aaaaaacagc catcccaaga tcaatgtcca ctgatctttc aaatatttaa
    15901 aaaaattcat cttaaccgct catcctttca gggaacgata tatccctgtc ctacttttgt
    15961 ttcgatatat ccttggatac ctgcctgttc gatttttcgg cgtaaccgat tgatgttgac
    16021 tgtcaatgta ttgtcatcta caaatcgttc gtcatcccac agtgctcgca gcaatttttc
    16081 ccgacttaag atctttccat tgtgcttcat caaaataaat aatagacgat attcattttt
    16141 gctcaaatca atgatctctc cgttgatttc catacttccg ttgtcaacat tcaacgtaat
    16201 gccattgtgg ctcatcattt cactagataa ttctgtgtag ttgtaagaac ggcgaagtaa
    16261 tgcattgatt tttgcaatca gtacatcgat ttggaaatgt tttgtcacaa aatcatctgc
    16321 ccccatgttc atagccatga tcatatccat attcgtattc ctgctggaga taaaaatgat
    16381 cggtactttc gatacttcac gtattttttg acaccaataa tagccatcca aaacgggaag
    16441 attaatatct agtaatacaa gctgtggttc ttctctttca aagtctgaga acacttgatt
    16501 gaaatcggtc gttccaaagg tttcaaattg ccatttctca agttcctcca tgatcaattg
    16561 tcgaatgatc tcttcgtctt cgactaccat gatttttgcc ataaaaaatc cctccatctt
    16621 tcttcttgct actacaatat caaagaagcc cttgtgaaac aagttggctt acctggaaaa
    16681 tgtcatttta gcaaacgtta aaaaaaactg gaaatatgcg atttaatcgc gtttgtctct
    16741 tccaagcagc ttaaatttat tatgttattc ttaataattg gtctattatc tgttattttt
    16801 ctttcgttgt ttttgtcttt taaattttcg ataacaccaa taaccgaaaa gcgttagcac
    16861 tagcaatata cctagaagga gtataagtaa tcgataaaga tggtattttt ttgtctgttc
    16921 tatctttgaa gagacttctt ctgtaaaggg aactcgtctt cctgtcacta gtaatcggtg
    16981 agtgttcacg gtgtaaggtg tacaagtgat aagtgtgact agatcctgct tttctctaat
    17041 tgctaatgta tttgtttttg ttggaaggac aacaattttc tcaacgactt cataagcgag
    17101 catccgacca tacacttcaa gatagaatcg gtcatcttgc tccatttctt caagatccgt
    17161 aaagagtttt ttctcagcaa taccgctatg ggcggtaatc acggaatgtg tactctctcc
    17221 tccagttgga aaagaggtgc cttgcaggac tgtcgctcct ctatctaaca aaagatcatt
    17281 cgtttcatca aataaaggca agctaacatt gattttcgga atatagatgg ctccgatcat
    17341 atgttgttcg taatattctt ttcctggatt ttggacattt ctaaccgctt gctcaaaagg
    17401 atctttgact tgtcccattc ctgggattac tgatgtatga gcaagcgtct gatttttctt
    17461 ttcatgcact gctaattgtt gctcatgttt ttcttcttta tcgcgttcaa gctgcttttg
    17521 ataattctcg atccttcttt gatccaaata attgctgaga gcatctgcta aaaatggata
    17581 tgtaaaaact gcagctccgg caagaaacat cactgccata aataattgta ataaaagact
    17641 gatttgggtt tgaggtctat tacctatttt ctttttcatt ttcttcacct tcttatttaa
    17701 aaaaactgca gcaaccttta taaaaccgat tgctgcagct tatttttgtt gcttatactt
    17761 cagacttcat tttagattta cggtaccagc tgtacgctcc aatcatcaaa cttattccga
    17821 ttagtaagaa tgccaagagc ccgttcccac cagtagcagg aagtgacccg cgtttcatat
    17881 tcggtactct ttctggccct ggattttcta ctcctacgat ctgaatttct tcattatagc
    17941 ttccataaga gatgataaat tcagtaaagg ctgcctcacc tgttggaagt acataacctt
    18001 ctggcgccat ggtctctttc atttgatagg ttccataatc taacccttga acctcaaagt
    18061 atccatttcc atctgcgaca aattctgttg cttgtccgct tgttgcccac tcaattctat
    18121 ctgctcctga ttcataaaca gctaaaggcg actccccttt ataaaatatt gcgtattcag
    18181 aacggtcttc attccacaat tcgaatcttg catctttaag caattcactg cttgatgcat
    18241 catatttttc aaactttctt ccacctgttg tgactttctc tggcggctct actccttctt
    18301 cataatcaaa ttcatcattt cgtccgattt caataaccgc tgtgttgtta tggaaatcat
    18361 ccgcttctag ttctgtggaa accttcaaat tatacgtgat cgttaaatct cctccacgtg
    18421 catctgctaa tctacctaaa gtatctactt tggctactcc attttcttct gtcaaaatcg
    18481 tcacgacaaa cccgttgctt actacttcta ctatataatc ttgtcctttt gttaaaatcg
    18541 tctcgccggc tcgcacttct atttcctgat cataataagt tagaccaact gtaggcgtat
    18601 ctgtgatttt gtaatttttg ataacagcgc ttccgttttc aaggacagaa tcgatgaaat
    18661 aaggaatagg tgctgtgatc gtgtaagaag ccgtttttcc tctttctaga tcaatataag
    18721 aaatcgtttc ttgttctcca gcttcattaa taatcgttac ttgtctaagg tcgctttcct
    18781 ctacattcat ctgtttatct gcttctctga tttcgttttt cggatacaaa tgcacatctg
    18841 tattgtatgt gtctttccat gtagaaccat cccacacgcc atccaccata tcaggcatca
    18901 tcactggcat cataacaacc attggagacg cgattctgga aatatgagct ggcgaataag
    18961 tttctaagaa aagatagact ttatctctag aagaaggtgc ttcttccgtc acttggacgg
    19021 tagagaatac cgcttctcca gcttcattcg ttgtttgttt gtcaatactt gtcagtcgat
    19081 attttaaaaa ccaactttct gtgtgccgtt tttgaatcca ttcaatcgct tcggcagaat
    19141 taagaccgtc ttctcgttcc ggtgttaaag gattatctcg aatcaaatcg taatatactt
    19201 ccgttacgtc aaacaaatca aaatcgactc ctggaatatt ttcttcacca aaatccgtag
    19261 aaaccaatcc gctattcggt cgttcttccg gaacagatga gaaacctttt ttgtgtaatg
    19321 taatcgtcac ttcacttggt ttttgtgcga cttcttcggc tttggctagt tttccgttcc
    19381 cgaaaatccc tattcccgca gtaacgagta acagtacact cattcccgtg acaatcattt
    19441 ttttattcat ttttttctcc ccgttctttt tttatt
//
LOCUS       DAYYFU010000048        19126 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000048 DAYYFU010000000
VERSION     DAYYFU010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.049"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..703)
                     /locus_tag="ACHA34_001999"
     CDS             complement(<1..703)
                     /locus_tag="ACHA34_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8792612.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="ACHA34_002000"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="ACHA34_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="ACHA34_002001"
     CDS             1758..2837
                     /locus_tag="ACHA34_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8792613.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2834..3462
                     /locus_tag="ACHA34_002002"
                     /pseudo
     CDS             2834..3462
                     /locus_tag="ACHA34_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(3572..3799)
                     /locus_tag="ACHA34_002003"
     CDS             complement(3572..3799)
                     /locus_tag="ACHA34_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8792614.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="ACHA34_002004"
     CDS             complement(3902..6490)
                     /locus_tag="ACHA34_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFM8792615.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="ACHA34_002005"
     CDS             complement(6510..7169)
                     /locus_tag="ACHA34_002005"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8792616.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="ACHA34_002006"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="ACHA34_002006"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFM8792617.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="ACHA34_002007"
     CDS             complement(7942..8808)
                     /locus_tag="ACHA34_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8792618.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="ACHA34_002008"
     CDS             8917..9558
                     /locus_tag="ACHA34_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFM8792619.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="ACHA34_002009"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="ACHA34_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFM8792620.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="ACHA34_002010"
     CDS             complement(10999..11898)
                     /locus_tag="ACHA34_002010"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8792621.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="ACHA34_002011"
     CDS             complement(11900..12697)
                     /locus_tag="ACHA34_002011"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFM8792622.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="ACHA34_002012"
     CDS             complement(12675..13379)
                     /locus_tag="ACHA34_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFM8792623.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="ACHA34_002013"
     CDS             13510..14085
                     /locus_tag="ACHA34_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFM8792624.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="ACHA34_002014"
     CDS             14203..14880
                     /locus_tag="ACHA34_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFM8792625.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="ACHA34_002015"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="ACHA34_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFM8792626.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="ACHA34_002016"
     CDS             complement(16943..17941)
                     /locus_tag="ACHA34_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFM8792627.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="ACHA34_002017"
     CDS             complement(18167..18820)
                     /locus_tag="ACHA34_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFM8792628.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5525 a         4136 c         3324 g         6141 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagt
//
LOCUS       DAYYFU010000049        18740 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000049 DAYYFU010000000
VERSION     DAYYFU010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.050"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            256..531
                     /locus_tag="ACHA34_002018"
     CDS             256..531
                     /locus_tag="ACHA34_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792629.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="ACHA34_002019"
     CDS             544..2616
                     /locus_tag="ACHA34_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8792630.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2784..4667
                     /locus_tag="ACHA34_002020"
     CDS             2784..4667
                     /locus_tag="ACHA34_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8792631.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            4679..5626
                     /locus_tag="ACHA34_002021"
     CDS             4679..5626
                     /locus_tag="ACHA34_002021"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFM8792632.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="ACHA34_002022"
     CDS             5646..6173
                     /locus_tag="ACHA34_002022"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8792633.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="ACHA34_002023"
     CDS             complement(6236..6889)
                     /locus_tag="ACHA34_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFM8792634.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="ACHA34_002024"
     CDS             7021..7863
                     /locus_tag="ACHA34_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8792635.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="ACHA34_002025"
     CDS             8021..8917
                     /locus_tag="ACHA34_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8792636.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="ACHA34_002026"
     CDS             8920..9633
                     /locus_tag="ACHA34_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFM8792637.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="ACHA34_002027"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="ACHA34_002027"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8792638.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="ACHA34_002028"
     CDS             10168..10395
                     /locus_tag="ACHA34_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFM8792639.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="ACHA34_002029"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="ACHA34_002029"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8792640.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11723)
                     /locus_tag="ACHA34_002030"
     CDS             complement(11181..11723)
                     /locus_tag="ACHA34_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8792641.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="ACHA34_002031"
     CDS             complement(11960..12358)
                     /locus_tag="ACHA34_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8792642.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="ACHA34_002032"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="ACHA34_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFM8792643.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="ACHA34_002033"
     CDS             13471..14238
                     /locus_tag="ACHA34_002033"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFM8792644.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="ACHA34_002034"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="ACHA34_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFM8792645.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="ACHA34_002035"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="ACHA34_002035"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFM8792646.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="ACHA34_002036"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="ACHA34_002036"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFM8792647.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5092 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcgttttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacatcggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctcttttt
//
LOCUS       DAYYFU010000050        18014 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000050 DAYYFU010000000
VERSION     DAYYFU010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.051"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..727)
                     /locus_tag="ACHA34_002037"
     CDS             complement(29..727)
                     /locus_tag="ACHA34_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFM8792648.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(720..2072)
                     /locus_tag="ACHA34_002038"
     CDS             complement(720..2072)
                     /locus_tag="ACHA34_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFM8792649.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2295..3695)
                     /locus_tag="ACHA34_002039"
     CDS             complement(2295..3695)
                     /locus_tag="ACHA34_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8792650.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3856..4593
                     /locus_tag="ACHA34_002040"
     CDS             3856..4593
                     /locus_tag="ACHA34_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFM8792651.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4718..5197
                     /locus_tag="ACHA34_002041"
     CDS             4718..5197
                     /locus_tag="ACHA34_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFM8792652.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5194..5961
                     /locus_tag="ACHA34_002042"
     CDS             5194..5961
                     /locus_tag="ACHA34_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFM8792653.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6191..6760
                     /gene="lepB"
                     /locus_tag="ACHA34_002043"
     CDS             6191..6760
                     /gene="lepB"
                     /locus_tag="ACHA34_002043"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8792654.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6864..7466)
                     /locus_tag="ACHA34_002044"
     CDS             complement(6864..7466)
                     /locus_tag="ACHA34_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8792655.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7572..7802
                     /locus_tag="ACHA34_002045"
     CDS             7572..7802
                     /locus_tag="ACHA34_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792656.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7793..8041
                     /locus_tag="ACHA34_002046"
     CDS             7793..8041
                     /locus_tag="ACHA34_002046"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8792657.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8147..8731
                     /locus_tag="ACHA34_002047"
     CDS             8147..8731
                     /locus_tag="ACHA34_002047"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM8792658.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8734..9807
                     /gene="prfA"
                     /locus_tag="ACHA34_002048"
     CDS             8734..9807
                     /gene="prfA"
                     /locus_tag="ACHA34_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFM8792659.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9800..10639
                     /gene="prmC"
                     /locus_tag="ACHA34_002049"
     CDS             9800..10639
                     /gene="prmC"
                     /locus_tag="ACHA34_002049"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFM8792660.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10632..10811
                     /locus_tag="ACHA34_002050"
     CDS             10632..10811
                     /locus_tag="ACHA34_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792661.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10833..11858
                     /locus_tag="ACHA34_002051"
     CDS             10833..11858
                     /locus_tag="ACHA34_002051"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFM8792662.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12033..13277
                     /gene="glyA"
                     /locus_tag="ACHA34_002052"
     CDS             12033..13277
                     /gene="glyA"
                     /locus_tag="ACHA34_002052"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFM8792663.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13430..14377
                     /locus_tag="ACHA34_002053"
     CDS             13430..14377
                     /locus_tag="ACHA34_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFM8792664.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14511..15140
                     /gene="upp"
                     /locus_tag="ACHA34_002054"
     CDS             14511..15140
                     /gene="upp"
                     /locus_tag="ACHA34_002054"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFM8792665.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15279..15521)
                     /locus_tag="ACHA34_002055"
     CDS             complement(15279..15521)
                     /locus_tag="ACHA34_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792666.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15642..15714
                     /locus_tag="ACHA34_002056"
     tRNA            15642..15714
                     /locus_tag="ACHA34_002056"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15675..15677,aa:Thr,seq:cgt)
     gene            complement(15833..16471)
                     /locus_tag="ACHA34_002057"
     CDS             complement(15833..16471)
                     /locus_tag="ACHA34_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFM8792667.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16594..17226)
                     /locus_tag="ACHA34_002058"
     CDS             complement(16594..17226)
                     /locus_tag="ACHA34_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8792668.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17577..17876
                     /locus_tag="ACHA34_002059"
     CDS             17577..17876
                     /locus_tag="ACHA34_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8792669.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6166 a         3154 c         3558 g         5136 t
ORIGIN      
        1 ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga tagggcagat
       61 ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct gccaggttct
      121 catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg taaatgacac
      181 cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc aaaggtcgtt
      241 caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa gtttcattga
      301 attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga cttaacgtgt
      361 agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg tagtagtgac
      421 ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg atatagttga
      481 tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca aagtcttgac
      541 caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt atactaacga
      601 tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc aaaaggtttc
      661 cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg agttcataat
      721 tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt acgaagctgc
      781 aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc tttgattttt
      841 tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat cccggcaact
      901 tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc tgggatatcc
      961 atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat tccgtctgcg
     1021 taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc aatcatatcg
     1081 attacttgat tgatacctac aggatttttg acaagtacta atgtacattt tttcccattg
     1141 atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc ttgtttgatt
     1201 ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc attgtagaca
     1261 ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa atctgctgac
     1321 acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg tttgaagtca
     1381 caatttggac agtaatattt ccctaagttt gcataagtga tcattttata atgcaagatg
     1441 tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg ttcacgatct
     1501 tcttcatggt caaagccata atattttcta ggattgactg tttcgataga attgaagatt
     1561 ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc gtctacaatc
     1621 attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa aatatttgta
     1681 aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc ttcatctatt
     1741 tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa agtcgataca
     1801 attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa ttgctggcgc
     1861 aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt gacaacgacg
     1921 acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag cgctagttta
     1981 cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga cgtttttcca
     2041 actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac gatttgctga
     2101 cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc ttttcttatt
     2161 cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat gatttaacaa
     2221 aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta ttccctctta
     2281 cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata gcctagatct
     2341 cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg cccaataaac
     2401 atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat ccctaaagat
     2461 aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag cgtttgttct
     2521 tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag cataaagaca
     2581 acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt gatgttatct
     2641 tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt tgttcgaatc
     2701 ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa aatgatcagt
     2761 gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg agtcgtaatc
     2821 gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa taaatggtcc
     2881 ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa agctaacaca
     2941 gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg tttgtataat
     3001 agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa aatcaaaaac
     3061 atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc cccagtgacg
     3121 tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa aacggcatca
     3181 cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt cccaaaaagt
     3241 aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat accagacaaa
     3301 cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat ccgtcgttta
     3361 gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac ttgaaataat
     3421 atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa agtcgatagt
     3481 ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga aacatggcta
     3541 tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt catcaatata
     3601 taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt ctgtcctcgc
     3661 cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg acaatagtcg
     3721 tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa cagataacaa
     3781 tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga aaaaagaagg
     3841 aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa aaatcaaaaa
     3901 ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga agaagtaaag
     3961 cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt ggaattcatg
     4021 tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc taaaagtgag
     4081 tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga gaaaactgac
     4141 catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa aatggtttta
     4201 acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg cttcaatatg
     4261 cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt tggaagtgcc
     4321 ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat ggatatccaa
     4381 aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg ctatgaacgc
     4441 tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg ttgcgtgttc
     4501 gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa agcttactta
     4561 aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa acgtaagaaa
     4621 aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt agtaaagtaa
     4681 ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag cacctatttt
     4741 gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag gtattgtgaa
     4801 tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa caacaccaac
     4861 tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa ccactacgac
     4921 aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata caactgacag
     4981 ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa ccaatccaag
     5041 tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga ctgtagatga
     5101 taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt caggaaatac
     5161 ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga ccttatcaac
     5221 ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa ccgtggatat
     5281 acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg actttggcaa
     5341 aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct tgtttatttt
     5401 actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga tgtcactcag
     5461 ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga atcgatagat
     5521 aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta tgacgaagat
     5581 tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga gatcaaaaca
     5641 aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta tccttattac
     5701 tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag ctactcggac
     5761 atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga acctgtcgga
     5821 ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga gccttatcgt
     5881 ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac cagtggttta tagtcgtagc
     5941 caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg cgataaaaac
     6001 gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt ttatataacg
     6061 agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca ttgtagaata
     6121 aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa atacaagtag
     6181 gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg tattagcggc
     6241 tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc attcgatgga
     6301 ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact atgaacgaat
     6361 ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta aacggatcat
     6421 cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta atggcaaaaa
     6481 gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata agttacaagg
     6541 agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca cctttacgag
     6601 tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata accgcttgat
     6661 ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag gaaaagttgt
     6721 tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata aaaagaagct
     6781 gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt cttggagttg
     6841 ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa aagcgaagcg
     6901 tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc agagacaaag
     6961 cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg gactttcgga
     7021 tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc aggagctaaa
     7081 atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag tacaagtgat
     7141 agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc gtggatcgtt
     7201 cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag catagtttct
     7261 gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact aacaagccca
     7321 gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag ataagtagtt
     7381 agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata atggttagcg
     7441 atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt tgaaatttta
     7501 acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa aagggagaat
     7561 ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga aagtgtgtcc
     7621 gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg gaaaaatata
     7681 tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa tatttaggct
     7741 ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt gtatgggaat
     7801 aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag cagaaggtta
     7861 cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc actactctat
     7921 atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg aagtttttcc
     7981 aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg aacaaaagtg
     8041 agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa aaatgctgaa
     8101 aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg cacaactatt
     8161 ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag tcgcacataa
     8221 ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata caagagatgg
     8281 tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga tatttgaaga
     8341 aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg tgctgatcga
     8401 tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg tggacgaatt
     8461 ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt tatttgaagg
     8521 atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa tctgttggtt
     8581 ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg tttatgaagg
     8641 cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa aacattactt
     8701 tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca agcaattgaa
     8761 gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc tgatacaaag
     8821 cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga agtttatcgt
     8881 cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg tgaaaaactg
     8941 gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa agaaaaagaa
     9001 gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga cgataaaaat
     9061 atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt cgctggcgat
     9121 cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga agtgatggaa
     9181 gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac cggagacaac
     9241 gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt tccttcaact
     9301 gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc agaagctgaa
     9361 gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca tgcttcaggt
     9421 gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca cttaccaaca
     9481 gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga aaaagcaatg
     9541 aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag cgagtatgat
     9601 gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg gacgtataat
     9661 ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa attagatcag
     9721 attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga tcaaacatca
     9781 aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt cacgggcttc
     9841 ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat ttttggaacg
     9901 aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt cagaagaaga
     9961 acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac ctcagtattt
    10021 gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa cactgatccc
    10081 tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg gtgtaccgtt
    10141 agaagtgata gatattggga cagggactgg agcaattgct attagtttga aagcagcaag
    10201 aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag tagcgaaaga
    10261 aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat taggaccagt
    10321 aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc ggaatgaatg
    10381 ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt ttgctgagaa
    10441 cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac taaaatcaaa
    10501 tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa aaatatttca
    10561 gcaagcattt ccagataaaa aagtggcgat aaaaaaggat ctttttggca atgaacgaat
    10621 gatccaagtt tatggttgaa gttgtggatg aaaaattttt tatttgtttt tttattataa
    10681 aaaaacactt atttgacaag gatgtaaacg actttttggt ttatcaacaa gtttatccac
    10741 tattgacaaa tagattatcc acaaacagtg gaaaacttga ggataaactc agacaaaata
    10801 agaatgtatg aaaaaggggt tgtggataaa ctgtggaaac gaaacgatat gatgaaacag
    10861 aattgcaaga ggcagccgaa gcactgaaag aaggagaact tgttgctttt cctaccgaaa
    10921 cagtatacgg attgggcgcc aacgctcttt taccaaacgc tgtcaagaaa gtgttttctg
    10981 taaaagggag accgcaagat aatccattaa tcgtccatgt cgcctctttt gaacaagtaa
    11041 aagagtatgt ggacaacttt catccggcag cagagaagat tgtgaagaac ttctggcctg
    11101 gaccattaac tttgatattt aagatcaaaa aagacacttt gccctcagtt gtgacgggag
    11161 gattatccac agctgcattt cgtataccgg ataacaaaaa aacactagaa gtaatcgaac
    11221 tttccggtgt tccgcttgtc ggtccaagtg caaatacgtc gggaaaacca agtccaacga
    11281 ctgcagatca tgtatatcat gatctacaag gaaaaattac cgggattatt gatgatggag
    11341 ctacgcgaat tggtgtagaa tccactgttc ttgatttaag tgatccaaca gccatgccta
    11401 tgattttacg accaggtgca gtaacgaaag aacaaatcga agcagtcatc gaatctcctg
    11461 ttgctattga tcagcatctt gtaaaagaaa atgaaacacc aaaagcccct gggatgaaat
    11521 acaaacacta ctcacccgat acacgtgttc tgatggtcag agatggcgat tggtcaactg
    11581 cagttcaatg ggcaaaaaat aagaagatcc gtgcaggtgt catagcaagc cccgaaattg
    11641 cggatcaagt gcggacagat actgcagctg tttatatgta taacgacaac tcagtagaag
    11701 cggcggctaa aggattgttt gcaggtctaa gaggattaga tgaaccaaca cttggattag
    11761 atttgatttt tgtacaagtg tatccagaaa ctggattggg gaatgcttac atgaatcgct
    11821 tgaaaaaagc agcaggacaa aattattttg aaaaatgatc aattggttat tgtcgcattc
    11881 ttttagtatg atacaatgct atcaagtaaa tcttctatca gtgaacgaaa aactaagcgt
    11941 aactaatcgt aatagaagag atacgaatat ttggcaattt gtttttgatg ggaaaaataa
    12001 aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg tttgaccctg
    12061 atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg gagctgattg
    12121 cctctgaaaa ctttgtctcc gaagcagtga tggctgcaca aggaagtatc ttgacaaata
    12181 aatacgcaga aggttatcca gggcatcgct attatggtgg gtgtgaattt gtcgatatcg
    12241 ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt gcgaacgtcc
    12301 agcctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta gaaccagggg
    12361 acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga tcaccagtca
    12421 acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca acagaagtta
    12481 ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg atcgtagcag
    12541 gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc gcagatgaag
    12601 taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca gcaggacttc
    12661 atcctaatcc agttccttat gcagatatca caacgactac cactcataaa acattgcgtg
    12721 gccctcgagg cggcatgatt ttgacaaacg atgaagcatt agcaaaaaaa ataaatagtg
    12781 cggtatttcc tggtattcaa ggcggccctc tagagcatgt gattgcgggt aaagcagtag
    12841 cattcaaaga agcattagat cctgcattta aagaatatag tgaacaaatc atcgccaatg
    12901 caaaagcaat ggtcaaagtt ttcaatcaag caattggaac cagagtcatt tcaggggcga
    12961 cagataatca tctgatgttg atcgatgttc gcgagttagg tataaatgga aaagaagcag
    13021 agagcatttt ggacagtgtg aatatcactg taaataaaaa ctcgattcct tttgaaacac
    13081 taagtccatt caaaacaagc ggtattcgaa tcggcacgcc agcaatcact actcgcgggt
    13141 tcaaagaaga agatgctgta aaagtggctg aactagttgt caaagcatta caagcaaaag
    13201 atgataacgc tcaactagac gaagtcaaaa caggtgtacg cgaattaact gaaaaatttc
    13261 cgttgcataa aaaataaaaa tttaaattcc attagtaaac agaaaagttc aatgttgggc
    13321 ttttctgttt attttttggg aaaacggatt ctttgacaat atcggtgatg ctctctagta
    13381 tatgaggtgt aattttagtc ttcacactaa atctcaggga ggaaaagtca tgactgatcg
    13441 tatctcgtta gaaaaggaag cagtagcatt cagtgatgca aatgcgcctc atccgcgtat
    13501 ctatgaacta gcaccggaag aaggaagagt tttactagaa gaagtacaag aatcacctgt
    13561 gaacaaatat tccgtcgata ttgaagactt gatggtggat actggcgaat ggggaaaact
    13621 caatattcgt ctcgtacgac cagaagggaa tatggataaa ttaccagtta ttttttatat
    13681 tcatggagca ggctgggttt ttggcagtgc tcaaacccat gataaattga tcagagaact
    13741 tgcggttcgt acgaattccg tcgttgtctt tccagaatat agtcgatcac cagaagcaaa
    13801 atatccaaca gcaattgaac aaaattatgc tgtgttgcaa cagctgaata aattagccaa
    13861 gtcaaaaaat ctggatctcg agaatctaat cgttgcagga gattcagtag gtggtaacat
    13921 ggctacagtc atgacgatct taacaaaaga acgaaagggc ctcccaatat ccaagcaatt
    13981 gctgtattat ccagtcacaa acgctgattt tgatacagat tcctatcatg aattcggaga
    14041 aaactatttt ttgacaagag aaggaatgat gtggttttgg gaccaatata cgacgaatga
    14101 agaagagcgt gctgaaatca cagcttcgcc tttgagagca aatataaatg aactaaatga
    14161 tcttccttca gcaatgattt taacgggaga agcggatgta ttaagagatg aaggtgaggc
    14221 ttatgcgcgc aaacttcgtg atgcaggggt tcctgttaca caagtacgct tccaaggaat
    14281 catccatgat tttgtcatgg taaatgctat ggatcagaca aaggcaacgc gtgcagcaat
    14341 ggcattatct acttcttgga tcaagaaaaa agaataagct ttgcttgtcc gcaggaaaac
    14401 ttcttttcaa aaacggattt tcttaggaaa aatggcgagt ttgactagtc aatcacttga
    14461 ttttgtttta caattaaaaa gaaacgacag aacaaaggag agcttaaaaa atgggcaaat
    14521 ttcaagtgat cgatcatcca ttgattcaac acaaactaac tattattcgg gacaaaaact
    14581 gcggaacaaa ggtttttcgt gaagtagtcg atgaaatcgc tatgttgatg gcatatgaag
    14641 tatcacggga tatgccattg gaagacatcg tgatcgaaac accgatcact gaaacaacac
    14701 aaaaaacgtt gtctggtaaa aaagtagcga ttattccaat cttgcgcgca ggtattggaa
    14761 tggttgacgg tatcttggaa ttgattccag ctgctaaagt gggacatatc ggtctttatc
    14821 gagatgaaga aacattggaa ccacatgaat atttcgtcaa acttccagaa gacattgatg
    14881 cacgtcagtt gtttgtagta gatccaatgt tggcaacagg cggatcagca atcatggcaa
    14941 tcgatgcatt gaaagaacgt ggcgcgtcga acattaaatt tgtttgtttg gtagcagcac
    15001 ctgagggagt aaaagctttg caagaagcgc atcctgatat tgacatctat acagcttctc
    15061 tagatgaaaa attgaatgaa catggttata tcgttcctgg actaggggat gcgggtgacc
    15121 gtttgtttgg tacaaaataa gtaaatacga aaaagggtgt gacacaactt ttgtcacatc
    15181 cttttttggt catttttaac gtttgatgca gaacagcttt ttcacagctt tcgtttgcat
    15241 ataaaaaaac catcccaaaa atgcagagat ggtcaaattc aaatcttttt tagttctttg
    15301 tagttaacga ttactacacc cttactttgc gtcatcaagt tcgcattttc tgcagtttca
    15361 tcaatctcaa caacagcgga atttttcaat tgctttgaaa tcgttccagt ttgcgacttg
    15421 ccgtgaattg tgaacattac gtgagtattg atcttgaatt tttcaccaat ttccgatggt
    15481 acgatagttg tatcaaattt tccaaaagtt gccattttca taaaacctgc ctttcacatc
    15541 aattatacca cgttttatga aaaatatcat gaaattaaac taattttcca tcggactctt
    15601 tacaatcagc caaatagagt atataatttc ttttggataa ggccagctta gctcagttgg
    15661 tagagcaacg cactcgtaac gcgtaggtcg taggttcgat ccctatagct ggcattaaaa
    15721 gagaagacag atatatcaag gatttttaat ccattgatac atctgtcttt ttgtttaaag
    15781 gaaataaagt atgctttctt agcaatagag catgtattat ttctataaaa atttaggaga
    15841 ataaaacgac tagcaatata gctgtgaaat caaatatcac atgaactgcc caacttgtaa
    15901 tgatcgagtt gcttttaatg gcagcttggt tgaataacaa tcttgccacc ccttgtatca
    15961 aaaggatgtg tgctacagtg tgtaaaatat ttccattatc ataagtactg taatggataa
    16021 atccaaagat gattgctgac aagaaactag cgacaggaat gctgaactta ctagaaaaaa
    16081 tagcgagaaa atacactgag aacaattctt ctccaagtat catgataggc aaaataataa
    16141 ataataacgg gctgaaagcc tcatttattg gattgcctgt taaaccccat ttcaaaattt
    16201 gttgcaaaaa taccgagact gcggcactga ttactacatt gataaaaaaa tatttggcta
    16261 tatttttgac aggagcgaca ggtttcgaga acattttttt cgtatctttt ttaccaaaaa
    16321 caagtgtaaa tgctataaat ccagtgatag ttaaagaaaa aatgctaata atggatggcg
    16381 caaatagcaa ccctaacaaa ccgagtacaa cgacaatagt gcccaatatc ccttttttaa
    16441 gttctatttt ttttcctaaa atgttattca tgataataac tcctactatt cagtttttga
    16501 agatagagtc agtatacatg aagagttaaa ataggcaatg tttttttatg gatatttgta
    16561 ttgctaaaaa tttatcttat atattcattt tgattatact acttttattt gtaaaaattt
    16621 tctagcaaaa ccaagtgctt gcaactgatt aagcgtacaa ttttttagat gttccgagcg
    16681 tacttcaatc atttcaaagg agttgctact taaatcgata ttttttaaat cagtatcaaa
    16741 aaagctagca ttttctaaat cattagtaga aaacattatt ttagtgaggg aacagtctac
    16801 aaaatctgcg cttgataaga cagagtgctc aaaggtactt tcttttatgt ttgaaccaga
    16861 taaatttaca tagtccattt tacaattctc gaataacgtg ttcccaatat aactttccat
    16921 caatttagta cctgttaatt tacaattgac aaatttcacg cgatgcaaca tgctttttgt
    16981 caatatacag cctgaaaaat cacagttttc gaatatacag tcggtaatct ccattctttg
    17041 aaaatcattg gaagcaaaaa atgcatactt gaaaatagat ttgtaaataa ttggatggca
    17101 gattatttgg ttttcgatga cctctttttg tgaaaggata ttatacagtt caaaatcttc
    17161 gttttcatag atatgaaaaa aggatccttt attcaagctt gcttcattaa ctttaggaaa
    17221 aatcatattt aattacctcc attttcataa agtcatatta acaaaagcta aaccaactta
    17281 cattagcaga aaatgagata ggttttcgcc tttttcgata acgatagtgt cgctatctct
    17341 tgaaacgttc ttagaattgc cctgagagac acaaattagg cgtaattcta ataaaaatct
    17401 tagaataaaa atttttactg atttaattta gaatgttttt aaaataaaaa tcgttgccat
    17461 aacagtgtat tgaaaggttt taacaatcca gtaatgattt aaaaaaatct agatgtagcg
    17521 tatactttgt ttatagaatg tagtgggaag aaatatattt ttgggaggaa atcgtgatgt
    17581 ctaaaagtga agaaatagaa aacatggtac taaaattata tgatctcgtt ttaaatccag
    17641 agatcaaaga aaaggaacgt atacttttga tagacgcaaa gacaggctta gaaaaaggtc
    17701 agtattatcc taaagtcata aataatttgg aacgtagttt acgtccgtta gcaatcaggg
    17761 gagaattgtc taaaccagta tcaccgtttt atatggagat atcaacaata ggaaaatttg
    17821 aaaaagaatt aggtcgagga atggcttcgg caccgattac ttttgggcat ttataaaaac
    17881 gagttattca tctcaatgaa ttaaaaagta tagtcaatct cataatgcag gctaggttca
    17941 tttatttgaa cagagtctgt atttttttat tctaaataaa gaaacagatg tttctttata
    18001 ttgtatttta tcct
//
LOCUS       DAYYFU010000051        17175 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000051 DAYYFU010000000
VERSION     DAYYFU010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.052"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..1371)
                     /locus_tag="ACHA34_002060"
     CDS             complement(61..1371)
                     /locus_tag="ACHA34_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFM8792670.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="ACHA34_002061"
     CDS             complement(1383..2801)
                     /locus_tag="ACHA34_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8792671.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="ACHA34_002062"
     CDS             complement(3041..4609)
                     /locus_tag="ACHA34_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFM8792672.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="ACHA34_002063"
     CDS             complement(4613..4894)
                     /locus_tag="ACHA34_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM8792673.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="ACHA34_002064"
     CDS             complement(4891..5814)
                     /locus_tag="ACHA34_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFM8792674.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="ACHA34_002065"
     CDS             complement(5811..6689)
                     /locus_tag="ACHA34_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFM8792675.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="ACHA34_002066"
     CDS             complement(6676..8013)
                     /locus_tag="ACHA34_002066"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFM8792676.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="ACHA34_002067"
     CDS             complement(8016..9119)
                     /locus_tag="ACHA34_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8792677.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="ACHA34_002068"
     CDS             complement(9193..10701)
                     /locus_tag="ACHA34_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8792678.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="ACHA34_002069"
     CDS             complement(10667..11359)
                     /locus_tag="ACHA34_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8792679.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="ACHA34_002070"
     CDS             complement(11381..12355)
                     /locus_tag="ACHA34_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8792680.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="ACHA34_002071"
     CDS             12655..13527
                     /locus_tag="ACHA34_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8792681.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="ACHA34_002072"
     CDS             complement(14109..16307)
                     /locus_tag="ACHA34_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8792682.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..>17175
                     /locus_tag="ACHA34_002073"
     CDS             16539..>17175
                     /locus_tag="ACHA34_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8792683.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYF"
BASE COUNT         5104 a         3581 c         2810 g         5680 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttt
//
LOCUS       DAYYFU010000052        17150 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000052 DAYYFU010000000
VERSION     DAYYFU010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.053"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..1475
                     /locus_tag="ACHA34_002074"
     CDS             138..1475
                     /locus_tag="ACHA34_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFM8792684.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="ACHA34_002075"
     CDS             1523..3652
                     /locus_tag="ACHA34_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFM8792685.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="ACHA34_002076"
     CDS             3627..4316
                     /locus_tag="ACHA34_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792686.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="ACHA34_002077"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="ACHA34_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="ACHA34_002078"
     CDS             complement(5546..5740)
                     /locus_tag="ACHA34_002078"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792687.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="ACHA34_002079"
     CDS             complement(5790..6230)
                     /locus_tag="ACHA34_002079"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFM8792688.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="ACHA34_002080"
     CDS             complement(6234..7079)
                     /locus_tag="ACHA34_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFM8792689.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="ACHA34_002081"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="ACHA34_002081"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFM8792690.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="ACHA34_002082"
     CDS             complement(8703..9650)
                     /locus_tag="ACHA34_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8792691.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="ACHA34_002083"
     CDS             complement(9873..10223)
                     /locus_tag="ACHA34_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8792692.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="ACHA34_002084"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="ACHA34_002084"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFM8792693.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="ACHA34_002085"
     CDS             11646..11966
                     /locus_tag="ACHA34_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8792694.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="ACHA34_002086"
     CDS             11988..12452
                     /locus_tag="ACHA34_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8792695.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /gene="ytpR"
                     /locus_tag="ACHA34_002087"
     CDS             12657..13262
                     /gene="ytpR"
                     /locus_tag="ACHA34_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFM8792696.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="ACHA34_002088"
     CDS             complement(13301..14590)
                     /locus_tag="ACHA34_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFM8792697.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="ACHA34_002089"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="ACHA34_002089"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFM8792698.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15700..15825
                     /locus_tag="ACHA34_002090"
     CDS             15700..15825
                     /locus_tag="ACHA34_002090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792699.1"
                     /translation="MIDEKKCLKNLLEDMDDIEENYLNGLGKQIYFRYLSYLKMK"
     gene            15822..16250
                     /locus_tag="ACHA34_002091"
     CDS             15822..16250
                     /locus_tag="ACHA34_002091"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM8792700.1"
                     /translation="MRHSNFLAFLFAPNETHNLSDEFFKMFLKRYIDSNDDTKAAINY
                     FDALLNSYEDLIVYRENKNIDILLVSEKNKIVVCIENKILSSESGGQLNKYQIYIERN
                     YSNYKKIFVFLTPDGNEPTNPSWGIITYKNIVEESVKYFV"
     gene            16321..>17150
                     /locus_tag="ACHA34_002092"
     CDS             16321..>17150
                     /locus_tag="ACHA34_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFM8792701.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT         5459 a         3105 c         3467 g         5119 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata
    15661 cgtaaaattt tagttagaat aatggcaagg agaaaaaaca tgatagatga aaaaaagtgt
    15721 ttaaaaaatc tattagagga tatggatgat attgaagaaa actatctaaa tggactggga
    15781 aaacaaatat atttcagata cttaagctat ctaaaaatga aataagacat agtaattttt
    15841 tagctttttt gtttgctcca aatgaaaccc acaatttatc tgatgagttt tttaaaatgt
    15901 ttttgaaaag gtatatagat tctaatgatg ataccaaagc tgctatcaat tattttgatg
    15961 ctttacttaa tagctatgaa gatttaattg tttacagaga aaataagaat attgatattt
    16021 tattggtatc ggaaaaaaat aaaatagtgg tgtgcatcga aaataaaatt ctgtcttcgg
    16081 agtctggagg gcaacttaat aaataccaaa tatatataga acgcaattat tctaactata
    16141 aaaaaatttt tgttttttta actccagatg gaaatgaacc cacaaatcct agttggggca
    16201 taattactta caagaatatc gttgaagaga gtgtaaaata ttttgtgtaa atagaaaaaa
    16261 ggaagtccct tctgtagaat agagttacca caacacattc acagaaaaga ggacttccat
    16321 atgaacgatt ttactacaga aattatggaa atactaatca ataaaggtga tttggatgat
    16381 ttgtttcgtc gtcacctaga attggctatt aacgctttat tacaagctga attaacggta
    16441 tttcttgact atgaaaagta tgatagagct ggatttaatt caggtaattc ccgtaatggg
    16501 aattattcgc gctcttttaa aacagagtac ggtgaattga atttagtaat acctagagat
    16561 aggaatggag acttttctca acaaacttta cctgcttata aaagaaccaa tgactcttta
    16621 gaaaccacta ttattcagct agttcaaaaa ggaattacta tgtccgaaat ctctgatcta
    16681 atcgaaaaaa tgtatggtca ttattacaaa ccacaaacta tttcaaatat gagtaaaatt
    16741 gtgtctgaag atgtttcggc atttaaagaa agaactttag aagctaaata ctcagtcatt
    16801 tttatggatg ctactcatat tcccttaaaa agacaaaccg tatcaaaaga agctgtctat
    16861 atagtaattg gtatcaggtt ggatggaact aaagaggttc taggatttac tatagctcca
    16921 actgaatctg cttatgtttg gaaagagatt cttcaagatc taaaagaccg tggtttagaa
    16981 gaggttttgt tagtcgtaac agatggttta agtggtattg acgatagtat tcatagtgtt
    17041 tatccaaatg ctcaatttca acaatgttgt gtccatatat ctagaaatat cgctcataag
    17101 gttcgtgtta gtgatcgaca agaaatctgt agcgatttca aattagttta
//
LOCUS       DAYYFU010000053        16980 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000053 DAYYFU010000000
VERSION     DAYYFU010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.054"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..2606
                     /locus_tag="ACHA34_002093"
     CDS             135..2606
                     /locus_tag="ACHA34_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8792702.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2709..4445)
                     /locus_tag="ACHA34_002094"
     CDS             complement(2709..4445)
                     /locus_tag="ACHA34_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8792703.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4442..5146)
                     /locus_tag="ACHA34_002095"
     CDS             complement(4442..5146)
                     /locus_tag="ACHA34_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8792704.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5277..5780
                     /locus_tag="ACHA34_002096"
     CDS             5277..5780
                     /locus_tag="ACHA34_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFM8792705.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5740..6078
                     /locus_tag="ACHA34_002097"
     CDS             5740..6078
                     /locus_tag="ACHA34_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFM8792706.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6099..7517
                     /gene="ffh"
                     /locus_tag="ACHA34_002098"
     CDS             6099..7517
                     /gene="ffh"
                     /locus_tag="ACHA34_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFM8792707.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7629..8207
                     /locus_tag="ACHA34_002099"
     CDS             7629..8207
                     /locus_tag="ACHA34_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFM8792708.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8211..8732
                     /locus_tag="ACHA34_002100"
     CDS             8211..8732
                     /locus_tag="ACHA34_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFM8792709.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8811..9365)
                     /locus_tag="ACHA34_002101"
     CDS             complement(8811..9365)
                     /locus_tag="ACHA34_002101"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM8792710.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9618..9893
                     /gene="rpsP"
                     /locus_tag="ACHA34_002102"
     CDS             9618..9893
                     /gene="rpsP"
                     /locus_tag="ACHA34_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFM8792711.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9905..10156
                     /locus_tag="ACHA34_002103"
     CDS             9905..10156
                     /locus_tag="ACHA34_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFM8792712.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10281..11072
                     /locus_tag="ACHA34_002104"
     CDS             10281..11072
                     /locus_tag="ACHA34_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792713.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11242..11763
                     /gene="rimM"
                     /locus_tag="ACHA34_002105"
     CDS             11242..11763
                     /gene="rimM"
                     /locus_tag="ACHA34_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFM8792714.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11753..12514
                     /gene="trmD"
                     /locus_tag="ACHA34_002106"
     CDS             11753..12514
                     /gene="trmD"
                     /locus_tag="ACHA34_002106"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFM8792715.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12650..12997
                     /gene="rplS"
                     /locus_tag="ACHA34_002107"
     CDS             12650..12997
                     /gene="rplS"
                     /locus_tag="ACHA34_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFM8792716.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13129..14265)
                     /locus_tag="ACHA34_002108"
     CDS             complement(13129..14265)
                     /locus_tag="ACHA34_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8792717.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(14381..15295)
                     /locus_tag="ACHA34_002109"
     CDS             complement(14381..15295)
                     /locus_tag="ACHA34_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFM8792718.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(15386..15814)
                     /locus_tag="ACHA34_002110"
     CDS             complement(15386..15814)
                     /locus_tag="ACHA34_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8792719.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(15819..16193)
                     /locus_tag="ACHA34_002111"
     CDS             complement(15819..16193)
                     /locus_tag="ACHA34_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8792720.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            16479..16664
                     /locus_tag="ACHA34_002112"
     CDS             16479..16664
                     /locus_tag="ACHA34_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8792721.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            16676..16924
                     /locus_tag="ACHA34_002113"
     CDS             16676..16924
                     /locus_tag="ACHA34_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792722.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
BASE COUNT         5513 a         3034 c         3332 g         5101 t
ORIGIN      
        1 cctttttttt tgctatatga caggaatgaa aggtgtttgc aagcttaatg tgaggaacac
       61 tttccatatc tgttctatac tggataggag aagtttggaa atatccagaa aacgaaatca
      121 atcgaggtag ccacatgaag aataaatgga taaatcgaag taaaaaaaag aaagtaacac
      181 aaagatcatc agataaagtc atccgtcttt ttccatcgta taaagaagga ttaacggcag
      241 atcaagtcaa ggaacgaata gaaaaaggtg cagcaaataa ttcagtcgat cccacattca
      301 aaacaaatca gcaaatcgta ttggaaaaca tatttacgta tttcaatttg atctttctta
      361 tcttagccat tttgctttgt ttagtagaat cctataagaa cttgactttt ttaccagtta
      421 tcatagccaa tacaggaatc gccatttttc aggaaattcg atccaaaaag atcttggacg
      481 aactaaatgt tttaaataca gcaaaagtca atgtcatacg agacgggata gaacaaacaa
      541 tagatataga agaccttgta atagatgaca ttgttatttt agagactgga tatcaaattc
      601 cagcagatgc agaggtcgtg gaagggaaac tgcaagttaa tgaggcttta ttgacaggcg
      661 aagcagatga aataaccaaa gaaataggtg atccattaat gtccggcagt ttcattgttt
      721 ccggaaaagc ttacgcccga ttagacaaag ttggagcaga ttcgtatatt tcccagctga
      781 cagtcaaagc caagacaatg ggagaaggag aacagtcgga gatgattgct tctcttaatc
      841 aattgatcaa atggattggg atcatcatta ttcctatcgg tatcctgctt ttttctcaaa
      901 gttacttttt taatggaaac acgatcaaag aaagtgtcgt tgcaatggaa gcagcattga
      961 tcggtatgat acctgaagga ctgtacttgc tgacgacgat tgcattagca ttaagtgcga
     1021 ctaaactagc aaagcaaaga gttcttttac acaatatgaa aagcattgag acattggcaa
     1081 gggtaaatgt cctttgtgta gataaaacag gaacgatcac agaaaataaa atgtctgttc
     1141 aaaaaatgat cgtctctaaa aaacaagaaa taatcagtca gtcagatcaa ttggaagcat
     1201 gcattgctga ctatgcaaaa gcgatggcag gagacaatgc aacaatggaa gctgttaaag
     1261 catattttac aaaaaccact gatcaatcct atcaccatgt tattcctttt tcttctgtcc
     1321 aaaaattcag cagtgtagcg ttggcagaca aggtgtatgt tttaggagcg cccgaaatgg
     1381 ttttgcgcga tcaattgccg gaatatgcgg aagagtttgt ttctttcgct gaaaaaggct
     1441 atcgcgtgtt ggtctttggt gtgtacgatg ggattcttga agaaccagta ttgacagaag
     1501 cagtcacacc attaggctat attctgattg ctaatcctat cagaaaagaa gcaagagcca
     1561 catttgatta cttcaaaaaa cagcaagtag ccatcaaagt gatttctgga gataatccgc
     1621 tcaccgtatc caatgtagct gtccaagcag ggatttcaca tgctgatcag tacgttgatg
     1681 catctaaatt atcagaagaa gaatatgaag cagctgtcga aaaatataca gtttttggac
     1741 gcgtcaagcc agaacaaaag aaaatcttcg ttcaattgat aaagcgtaac aatacagtag
     1801 ctatgacagg tgatggcgtg aatgatattc tagccatgaa agaagcagac tgcagtatag
     1861 cgatggcttc cggaaatgaa gccgccatgc aggcctcaca agtggtctta cttgattctg
     1921 acttttcacg catgccagaa gttgttgcag aaggacggcg cgttgtgaac aatattgagc
     1981 gatcagccag cttgttttta gtgaagaata ttttctcctt tttgctctca ctgttttcag
     2041 tgatctttgc tctgacctat cctttagaac catcacaaat cacattgatt agtttgttta
     2101 ctatcggact tccatctttc ttgcttgctc tagaagaaaa caaaaagcga atcagaggga
     2161 aattcatcat gaatgtgatg gaaaaagctg tgccaggtgg actaacagat atgatcgtag
     2221 tcggtgcgtt ggtgatctgc ggggtgactt tgaatctaaa taaaacagat gtttctacag
     2281 catctaccat gcttttgatt gcagtagggt tcctcgttct ctataaaatc tgttcaccgc
     2341 tgaataaatt cagaagtcag atcattcttt tctgtgctag cggtaacttt ttttcggtca
     2401 tctttttgca taagctgttt tcaatcaccg gtatctcagc tgtatcactt ttgctgttga
     2461 gcattctatt tttctcggca gattcgattt ttaggcattt gaccacattg gtagaatatt
     2521 tgtttgaacg taaaaatgaa aaagagcaaa agccgtttag tcgcgtagct aaaaaatttc
     2581 tatcgatatt taaacgagaa aattgacgaa aagtatctca aaaaagagta aaaaagaaca
     2641 accactcgtg tatctttcga agcaggatga gtcatccaac tttaaaacgc acgagcagtt
     2701 gctctttttt acattttcgg aatggtaacg gtgaaagtcg aacctgttcc aagatgactg
     2761 tctacagtaa tcgtaccatt aaggagttgc acgtagtctt ttacaatcgc taaccctaat
     2821 ccggtcccgc ctgaatgtcg gcttcgcgcc ttgtctacgc ggtaaaaacg ctcaaagatc
     2881 cgttcctgat cctcaatgtc gatacctata ccaaagtctt ggacagaaaa cgatagatca
     2941 tctgttgcct gatagcggat aataatctcg ctgtcgcttt ttgaatattg gacagcattt
     3001 tcaattagat ttttgataat tggataaagg atatctgttt tagtagtaaa tggttcgttc
     3061 tttccaatca atcgaatcgt caaccgtttc ttttcgatga ttggctgata attttgaacg
     3121 atttgctgga aataaggttc tgtataaatt gtctggatct cgtaagaatt ctcgccatct
     3181 tttgacagct ggatgatttc ccgaatcagt ttatctaacc ggatcgcatc tttttgcatg
     3241 atctcaagaa aagaagtcag agtttgcggg tcatcttttg caccatccag taaagtctcg
     3301 gtaaaaccaa tcagtgaagt cacaggagtt tttaattcgt gagagacgtt gccaacgaaa
     3361 tccttttgta gcttttctaa acgtcgcact ttcgttaagt catacgccac ccctagaatc
     3421 tgtccggtac cttctgaatt agtaaagtag cgcaaactca agtctaatgt ctgccgacct
     3481 tctgtaaggg tgatctcctg atggatcagc ggatgctcag gagtcacttg atgaatcagc
     3541 tgaatgagtt ttggctcttg aatgacttct gtgtaccgct gttcaggttg atagcttacc
     3601 acgtttaaat gtagctgcat ctttggatta agcagtagta aacgagaatc agcatcatca
     3661 atcaggaata cgccaatcat cagttcgttc agtaacgtat ataattgctc ttctgtggta
     3721 gtacacgccc gatacatctt actcatttgt tcgctcaacg cattaatcgt ctgatacaat
     3781 tcttcccatt gatcgggtgt ttgcatgatt acttccgcct gattaggatt ttgcaccatt
     3841 ttttttagta cgggcagcac ggttttcaat ggctggtttt tttgatagat cagataatag
     3901 atcattcctg caagaacaag gaagaaagac agaaagaaga aaaagaccca acggcgaaaa
     3961 ctctcagttc tcggtaaaaa accgcttgtc ggttctgcga tacgtaaaat ggcttctaac
     4021 tggccacttt ttttgactgg taatgcgaca tataaaagat cctcattcaa agtagtactt
     4081 ttgcgcaaag cgctacctag gttcccacca ttcaatacgg ctttgatttc gggacgggag
     4141 tttcgctgtt cattcaatgt ttcattgttt gtgtcaaata agatctttcc agtagcatcc
     4201 attaaagtaa tccgttcgtt cgactgatgg acgaattctt ccaaagcagt tttgttttgc
     4261 gctgcttgga ggttttcgac atctaattgc cggatcagca gttgtgcttt ttttgtcagg
     4321 tagctttctt gctgttccac gatctgctgt tgaaaataat gggaaatcaa ttgccagcca
     4381 ccaaaaaaca aactaactaa aagaataaga aagaagagac gttcataaat ttttttccat
     4441 ttcatcgttt gggttcctga aacttatagc caaatcctct aatagtcacc aagtactttg
     4501 ggtgttttgg atctcgctcg attttttctc gcaaatgact aacgtgtaca tctacgattc
     4561 ggctttgtcc tgcgaagtca aaattccaaa tgcggtctag caatgtgtct cggtcgatca
     4621 cacggtcttt tcgtttcatg aaataaacaa gtaattcaaa ttctttcggt gttagttcaa
     4681 tcagatggtc gtctacggtc acttgataat tggtcaaatc agcagtgatt tgtccaatcg
     4741 atagatattc aggctcagct tcatcttttg attgcttacg aggttcaatt cgtctgaata
     4801 ttgctttcat acgggctaat acttcgcgag gactaaatgg tttggtcaaa tagtcgtcag
     4861 cacctatttc taggccaatg atccgatcca cttggtcatc tttagccgtc agcatcaaaa
     4921 tcggtgtttc atttttttct tgacgcagcc gctgagtgat ctccatgcca tccatcgttg
     4981 gaagcatcac atctaaaatg atgaaatcaa atgattgttc taatgctaat tctaatcctt
     5041 cgcctccatc tgtggcactg gttaccttgt agccttcttt ttccaaattg aacgtcaaga
     5101 gcgttacaat agaaggttca tcatcaacaa ctagtatttt tttcattcaa atctccttta
     5161 aataggtttc attctgttta tattttagca tagaatcagt acaaagggct attttacaaa
     5221 agaaataaat ggtataatag gaaggcactt tgctgaatag aaaggaagag cagtcaatga
     5281 ttggaatcag tgcatgttta ggcggtctgg cctgtcgcta tgatggacag aaaaaagaag
     5341 taccagaatt gatggaacta gttgaaactg gagaagctgt catggtctgt ccagaagtga
     5401 tcggcggatt accaattcct agagatcccg ctgaaatcat tggtggaaat ggattcgatg
     5461 tgtggaaaga tcaagcaaaa gtatggacga caagcggcga agatgtaacg gaagcgtaca
     5521 aaaatggcgc gatcaaagcc tatcaaaaat tgcaggaaaa acacattgat ctagtcatca
     5581 tgaaggaaaa aagcccttca tgcggaaccc attcgattta tgacggaagt ttttccggaa
     5641 taaaaaaaaa tggtgccgga gtggctactg cttattttat tcagcaaaaa atgactgtgc
     5701 tctctgatga agaatggctg aaaactagag gtgagcagaa tggaaatcga aaagaccaat
     5761 cgaatgaacg cgctctttga attttattcg accttattga cagaaaaaca aatgaactac
     5821 atggaattat attatgcaga tgatttttca ttaggtgaga tcgcggaaga atacgaagta
     5881 agccgacaag cagtatatga caatatcaag cggacaagca agatcttgga ggattatgaa
     5941 aaaaaactcc atcttttttc taattatatt gttcgagaac aggtattgga aaatatgact
     6001 gcatatatta cagataagta cccggaagat aaaaaactac ttgaatatgt acagcaaatt
     6061 caagcaatcg aagaataaat aaaaggaatg aagaaaatat ggcatttgaa agcttaactg
     6121 aacgtctcca gcaggcgatg ggaaaaataa gaaaaaaagg aaaagtatca gaagctgacg
     6181 taaaagaaat gatgcgggaa atccgccttg ccttactaga agccgatgtt aacttgcaag
     6241 tagtcaaaga ttttactaag cgcgtacgcg aacgtgcggt aggggcagaa gtgctggaaa
     6301 gtttatcacc tgcacaacaa atcgtaaaaa tcgttgatga agaattgacg atcacacttg
     6361 gttctgaaac agcagagtta aataaatcac caaaaatacc aactgttatc atgatggctg
     6421 gtcttcaagg ggcaggtaaa acgaccttta ctggtaaatt agccaattat ttaaagaaaa
     6481 atgaaaatgc tcgtccgctc ctaatcgcag gggacgtata tcgaccagct gcgatcgacc
     6541 aattgaaagt attaggtcaa cagctagacg ttcctgtatt tgatatggga actgaagtca
     6601 gcccggtaga gatcgtccgt caagggatgg agttagcaaa agaaaagaaa aacgactatg
     6661 tattgatcga tacggcagga cgtctgcata tcgatgaaac attgatggat gagttgaaac
     6721 aaatcaaaga attgacgcaa ccaaatgaga ttttgcttgt tgtcgatgcc atgactggtc
     6781 aagatgccgt caacgtagct gatagcttca accagcaatt aggtattaca ggggtcgtca
     6841 tcactaaact tgatggggat actcgaggcg gggcagcact atcgatccgc tcagtgacag
     6901 gcgcaccaat caaattcatc ggttctggtg aaaaattgac tgacttagaa gtattccacc
     6961 cagatcgtat ggctagccgt atccttggta tgggggatat gctgacattg attgaaaaag
     7021 cgcagcaaga ctacgacgag aaaaaagcgg aagaacttgc acagaaaatg cgggaaaatt
     7081 cattcgattt caatgacttt attgaacagc ttgatcaagt gatgggtatg ggcccactgg
     7141 aagatctaat caaaatgatc ccaggaatga atcaagtccc tggaatcgaa aacgtcaagg
     7201 tagatccaaa agatgttgaa cgcaaaaaag cgatggttta ttctatgacg ccagcagaaa
     7261 gagcaaatcc tgatctgttg aacccaagcc gtcgtcgccg aattgctgcc ggttctggaa
     7321 acagtgttgt tgaagtgaat cgaatgatca aacaattcaa ggaatcaaga aaaatgatgc
     7381 agcaaatgtc caaaggggat atgaacatcc caggtatgga ccaaatgttt ggttcaggcg
     7441 tcaaaggaaa actaggcaaa atggcgatga atcgaatgat caagaaaaat aagaagaaaa
     7501 agaaaaaacg gaaataagat gaaaaaaaca gcttcggtac agactgaaaa tgtctactgc
     7561 cggagctttt ctatcagcaa tttttattct ttactaatac aattactcta aacgaagagg
     7621 tggcacagat gagatgtccg tggagtgaac aaacagaaag tatgcggcaa tatcatgata
     7681 ctgtctgggg tgttcctgaa tatgatgacc aaaagctgtt ccgaaaattg atgcttgata
     7741 ttaatcaagc cggattgagc tggcagacga ttttaaataa aagtgaagca ttcgatgaag
     7801 catatgatcg attcgatatc aaaaaagtcg caaattatgg agaaacaaaa atcagtgaac
     7861 taatggaaaa caaaggaatt attcgtaacc gaagaaaaat cgaagcagcc gtccaaaacg
     7921 ccaaagcagt cttgaaaatt caagaagaaa tcggcagttt ttcagagtat ctttggtcgt
     7981 tttctaatgg aaaaatcgtt gattcttctt atcattcaca agaagaagtc ccaacaacaa
     8041 gtcctctttc agatgaatta gcaaaagatc taaagaaaag aggatttaaa tttatcggaa
     8101 gcacaacaat ctatgctttt ttagaagctg tcgggatcat caatgatcac cttgacagtt
     8161 gtttccgcaa agaagaggtc gtacgtatcg gcagaaaggg cgaataaagc atgcgaaaaa
     8221 tcatatttta tggtgctata agtttagatg gctatttggc aacgaaaacg gatgatcttc
     8281 aatggctttt tgacactcca acaggtaagc aaactactta tgaagctttt tatgaaacaa
     8341 tcgatacgac catcatgggc agaaaaactt atcaggaagc aaaaaaatat ttggagaccg
     8401 aagcaattta tccagagaaa aagaatttcg ttttttccac ccacgcacaa ttacaattgc
     8461 cagatgctga ggtcgtaaac gaagatcctg ttgcttttct aaaaaatcta caaatgacag
     8521 aaggaaaaga tatctgggtt gtgggtggcg gtcgattgat caaaccgcta ttggaacatc
     8581 aaatgattga tgaatggtat atccagatta cccctgtatt attaggggac gggattcctt
     8641 tattccaaaa aggaacatat gaagcgcgtt ttgaaatgct agacacgaca cgattcggtg
     8701 aatttatcga attgcattat gtacgaaagt agaagtaaat aaaataaaaa aggcaactat
     8761 atgcagaaat gtcctgtaca tagttgcctt ttttattacc gttatgcttt ttactctgtt
     8821 aatttgttag aaaaatctag atacgcatct aaaatcgttt ggaagaaact taatgttcct
     8881 tcaacgatat taccattttc atcaattaga ttcgcaacgt ttccgatata tgcttccggt
     8941 tgctgcaagg ttggcacatt caaaaagaca agtgattggc gtagatggtg gtttgcgcca
     9001 aagccgccga tcccacctgg tgatgcagta acgactaaac ctggtttatg atcccaaaca
     9061 ctgtgtccat aaggtcttga gcctacatct aaagcatttt tcaatactgc tggcacagaa
     9121 cggttgtatt caggagtaac gaaaacgaca ccgtctagtc ccctcacttc ctcacgaaaa
     9181 cgagtccatt cagcaggagc ctgttctgga gtttcgagat cctcattgta aaatggcaga
     9241 tcatcgattt ttacaaatac actttcgtac ccttcaggaa gcaatttcgc aaaagcttct
     9301 gctacttttt tgttgtaaga atcttttctt aaactaccaa taaaaaatcc aatttttttt
     9361 gtcatacgat ttttcctcct tcaaactatc tataataatc ttacaaaaga taagtaactg
     9421 gagcaaataa aaacacttac tgacaaaagc tacatagaag atagattact tatttttaac
     9481 aaaaaagaag tctaaaatct tctattttct aaaaaaaatc gaatttaatc aagctgtaaa
     9541 gaaaaaaagc tttacagggt aaaaaaaaac tgttacacta catcttgtga ataaaacaat
     9601 ggaggtggaa ataaataatg gcagttaaaa tccgtttaaa acgtatgggt tctaaaaaga
     9661 gtccgtttta ccgtatcgtc gtagctgatt cacgttctcc tcgtgatgga cgtttcatcg
     9721 aaactgtagg tacttacaat cctttgaaag accctgcaga agtagtttta aaagaagact
     9781 tagttcttga ctggttatca aaaggtgcac aaccttctga cactgtacgt aacatccttt
     9841 caaaagaagg cgttatgcaa aaacaccacg aagctaaatt ctcaaagaaa taaggtgacc
     9901 ggatatgaaa gatttaagcg acttagtctt aacaatcgtt cgtccgctag tcacttatcc
     9961 tgatcaagtc aagttagacg tcgtagagtc gaaagacttt tacgagtaca atctaacagt
    10021 ggctcctgaa gatattggcc gtatcattgg aaaacaaggg cgtgttgcta aagcaatccg
    10081 cacaatcgtt tacggtgttc ggatcaatgc accgaaaaaa gtgcgtttga atatcatcga
    10141 tgataaggaa aagtaaataa acgtgctgta ctcgaatttt cgagtgcagc ttttttgttt
    10201 tttttattaa ttgaagaaat gattatttgg caaaacaagc aagaaagcgt atccttatag
    10261 gaggagggag tgaacagcca atgagaaaga aaatgttatt tattccattt gctctattgt
    10321 taagcgcttg tagttcggtt actcaagaat cagacaatac acatctatca agcagtatat
    10381 ctgaaactgt taccagtcca gaaaaaagta gtaccaaaac ggcttccacc actgaacaaa
    10441 caatggctac ctcaaataat gagaaaaaga ctgctttaga ccagttgaaa gaacaacagc
    10501 caaatgttcc catgcctcta gatgtaccag tctcttcagg ctatctcaat atcgcagcga
    10561 cacatacaaa gcaaggttat tctattttat attacagaac agatcgtccc ctcgggttga
    10621 atgctgacga attgaatcaa gaaacgccta ttgcaaccta tctatatcaa tatgggtttg
    10681 cttctagtca agaaacaatc caagttttgc agccatttga gatcgacaca aatggtcagc
    10741 aagtggactt aggttctcga atcacagggt atcagcaagg tgcagcaggt tcaagttttc
    10801 ttgaatggca agaaggaaac tggcgtattc gaatccgtgg gaataatata gaaggccaag
    10861 atcccttact attagctaaa gaaatcgtag cttatttgga agaaaacagt ctaccagcac
    10921 ctgaacaatt tggtaaaatt actgttgata tgggtgatac gacgaatcga gccgtagaag
    10981 taagctggca agaaccgaaa aatgcctata cgatcactca tcaagatcct atgtctgcgt
    11041 tgaagatggc agtttctatg aagaggttgt gaaatcagcg cgctgatctg agcccatcat
    11101 ttaactccaa gtcacaggaa gtaatcaaaa atagcacgcc gcatttttgc ataattttgg
    11161 cttaatacct taaaagaaaa ctagaattgc ctcttcacac atgctaaaat aaaacagaca
    11221 aaaaagaaat ggggatataa gttgactgaa tacttgaatg taggaaaaat cgtaaacaca
    11281 caaggtatca aaggcgaagt ccgtgttatc tcaacaacgg atttcccaga agaacgttac
    11341 aaaaaaggaa cggtgctaac actattccaa gacggaaaag cacctgtgga attaacagtc
    11401 aaaagtcatc gcaaacataa aaattttgac ttattgagtt ttgaaggaca tccttctatc
    11461 aatgatgtgg aaaaatatcg ggatggcata ttaaaagtgt caaaagacaa ctctgtagat
    11521 ctagctgaaa atgaattcta ttaccatgag atcatcggtc tgcatgtatt agaagatgga
    11581 actgagttag gtaaagtaaa agaaatcttg tcaccaggtg ccaatgatgt ttgggtcgtt
    11641 caacgtccga aaaaagctga tatattgctt ccatatatcg cttctgtcgt caaagaagtc
    11701 gatttagaag ctggagtggt ccgtgtagaa attccagaag gactgattga cgatgaagat
    11761 tgatgtattg actttatttc caagaatgtt tgaaggtcct atgggggaat ccatcatcgg
    11821 taaggctgta gataaaggat tattagatat caatatctct aacttccgcg attattcaga
    11881 taacaaacat cagacagtag atgattatcc atacggcggt ggagcaggta tgctgctcaa
    11941 agtccagccc atctatgata acatccaagc cattgaaaaa gctgcaccgg atgtcaaaaa
    12001 aagagtaatt cttcttgacc cagctggtaa acgattcgat cagaaaatgg cagaagaatt
    12061 ttcacaagaa gaacacttag tattcatttg tggtcattat gagggatatg atgaacgtat
    12121 ccgcacatta gtgacagatg aagtttcctt aggagattat gtccttactg ggggagaatt
    12181 aggtgcgatg gtcatgatcg atgcaacagt tcggcttttg ccagatgtgt tagggaatca
    12241 gacttcagct caaacggatt cttattcaac cggtttattg gaacatcccc aatatactcg
    12301 cccggcagaa tataaaggga tgaaagtacc agaagtattg acaaatggaa atcataaatt
    12361 gattgaagaa tggcaattaa aagaatcact aagaaggacc tatcaacgtc ggcctgatct
    12421 tttggaaaca cttgaatgga cacctcaaat gacgaaattt ttagaagaaa tcaagaaaga
    12481 agaacaagaa aaaacagcag atgaagccaa gtgacttttt ctaaaatctt ctttacagta
    12541 ggttgataat atgttagtat tttatggtga gtgcaaaagc acttaactat tacgatattc
    12601 cgctgtgaga aaagctcata agaatatttg gaataaggag aagaaaacca tgaatccatt
    12661 aatcgaagaa ttaacaaaag aacaattacg ttctgacatc ccagcgttcc gccctggtga
    12721 cactgtgcgt gttcacgcaa aagttgttga aggtactcgt gaacgtatcc agttatttga
    12781 aggtgttgtt atcaaacgcc gcggtgctgg aatcagcgaa acttatacag tacgtaaagt
    12841 ttctaacgga gtgggcgttg aacgtacatt cccattgcat acaccacgtg ttgcaaaaat
    12901 cgaagtagtt cgctacggta aagtacgtcg tgcaaaattg tactacctac gtgcattaca
    12961 cggaaaagct gctcgtatca aagaaatccg tcgttaatca tcgttatcag actgagaaag
    13021 aacccttggt attcaagggt tctttttttg ttctctgttt ttttatatta acgatttcta
    13081 tttgggacat ttttgattaa taaaaagggg caaaaaaggg gcaacaagtt acagtttttc
    13141 tatatcttct ttcaattttt cttttactcc ctttgtcaca tgcaaataaa tcttttcagt
    13201 gatatcacta ttttcgtgcc caacgcgatc ctgaatagca tacagaggag ttcctagttc
    13261 cgctaatttt gaaatatggg tatgtctaaa aatatgtgaa ctaagttttt tatcaatctt
    13321 catgtcagct ttatgatttc tcaaataggt gttaattgct gttagttgga aaggagttcc
    13381 tttcgttgtt tgaaatagaa attgaccatt cggatttaat tctagaagct cattgtaaat
    13441 agctatcgct ttttttggta aatcaatttc tcgcattcca gcagcagttt ttgtagaatc
    13501 agattttttc atatcagcta ttgaacgttc tctatacatc atcgtcccat ttataactac
    13561 tgatgcatta ttattggtaa tgtgtacatc gtctttactt aatgcgattg cttctccagg
    13621 cctcattcca gtcaaatata gccactggaa aaggagagaa tatcttttat tgtgtgaagt
    13681 tgtgaaattt actagtcttt tatattcatc atcttctaaa aacttatctt taatttttat
    13741 cgtttttgac tctcgtttat aatcaataac tacttcgttg ataggatttt tttctacata
    13801 gccttttttc atagcgtatg aaaaaagaag attcaatttg gatttaatta cactgacgta
    13861 cttatttgac aagtcatctt tatatatcat atcttcaaaa atattattta aatctactgt
    13921 attaatacca gaaacgatgt aagtctcagg tatctttttc tttatagtgt ttaagatatt
    13981 attcgtagga taatatgtag actccttaac ttgtcttttg taaataacta accattcttc
    14041 gacaagctca tgaaatgtta gatcaggctt ctgaagtgtt ttttcgttta attttatatc
    14101 aattttttta tttaactcta gcaatgccac tttctgagtt tctcgagatt tatttttata
    14161 agtaatgctc actttttttc tttttctagt ttttggatca atatatcgct cattatattt
    14221 atatactttt tttccgtttt tatcagtttt ggtttcaatc cacattgttt tcatctccta
    14281 tttgttgcta taataggaat ggataagtaa gccaattata gcaggtttat ttttcatcac
    14341 gtccacaaac tttggcgagg gagggggcgt gtttttttgt ttagtagcta cctttccatg
    14401 aagtatctgc gaagaacact tcaaatgttg ttccatcatt tttcacttca aaatatgctg
    14461 ttccttgtgc ggatttaccc ggttggatgg tttccgaaaa gtaatctcta ctagctaaat
    14521 cagatttaat atctgatgaa tcataaaatt ctaaataact tgaacttgca tcaaaaggtg
    14581 tagtccctgt gttttctaca agaaaatcta cctttgcgta aaataggcct tctggtttat
    14641 agaaatcatc accgtaatct tttgatacag actggacagt tacgttaata gagttaccat
    14701 tttcagaagt aaaaggaact gcttctccta tactatgtgc aactgatgcc gtctggggtt
    14761 ctgaagacga agttgtagtt gtagttgtag tagagcttga atctttggat tttccatctt
    14821 tatctaagaa agttcgttga ttataatata caacctgttc agtttttgga ctaacccaaa
    14881 ttaacattgt ttcatcatca gaatcagttg aataagtata ttggagcatc tctaattttt
    14941 ttatatcttt tgcactattt ccatgttcaa ttaattcaga gtgctcttta taataatctc
    15001 cttgtgcatc ctttgaaata gatacaatta attcatcccg tgtgatagca ttgtctaatt
    15061 ctttgtaaac aaaatcattt gtccatttac gtaacggtga accaagtgtc tgataaactt
    15121 gagttaaatc atcaccaact tttattttat gaacgtctga tgaagttagt ttttttgttt
    15181 catattcaga tgttgatgaa acagtatttt ctttagtgct agcagaatta ggactagtgg
    15241 agcatccgct taataccaag cctaaaaaaa gcaacccaaa aacaattttt ttcattttta
    15301 attcctcact tctatgatat gatttttttg taggatctta gaaataaggt ttttagtccg
    15361 tgttgcagca cggacttttt ttacattata taagaaagcg ataagcgctt tctggaagtc
    15421 cgtaaagatt ctttaattcc tcaattcgtt taggatattg atcattgtct tctttgtata
    15481 gagaaacaat gagattagca gcaaagcaat tagcttcgct ttcagatttg cttctagatg
    15541 ttcttgttga tacatagtaa ctggataagc cacgatgaaa aatagcgtga cctaattcgt
    15601 gagcgcaaat gtagaatctt tcctcagagt ctctcagttc atcatttaag aagattattg
    15661 cacgacctct aatttcttga aactgtcctt tgggattctc gataaaagga acgtattgaa
    15721 ttttaatgcc catcttttca caaatataaa aaggattagc ggactggtat ttccgcttca
    15781 actcctcgac taaattaatc gtatccatct ccataagctc acatcttttt gcctttttct
    15841 ttgtcttctt tcacaatatc ccagaaggtt ccaataagga tatcttttac gcgttggacc
    15901 tgttcaggcg tcagtgtttc tccgccataa gacatattga catttgaatc tagtaattta
    15961 tcaagttcaa ctacttcatc ttttgttgcc cagtcaggaa cttggtttct accgagtaaa
    16021 taatcagtag ttacattaaa ataatctgct acaagttgta gtcttttagt actaggagtt
    16081 ctatttttcc attgataaat cgtgttttta ggtatattta attcttcttc caattgagtt
    16141 atacttatgc ctcttttatg agctaattcc tttattctat ctagtaaatt cattttcgca
    16201 cctcacaagc tacgaaaaca ctaataaaaa aattagttaa aggtgttgac aactaataaa
    16261 agtgttagta tactgttttc gtaagctaaa atattagcta aaaagacttg ataactaata
    16321 aacacttcac ggtcggcaaa ctctgaaatg taaattacta ggcatttctg tgtcttattt
    16381 agctatgtct atatactaat aaaaatatta gttattgtca acgaatatta gctaattttt
    16441 tagcttacag attatttttt tagaaaggag ctatttttat gtctgagaat ttagacttaa
    16501 aaattcgagc ggagatgaga aaaagaagaa tgactttcaa agaactagct gcgcttatcg
    16561 gtatttcagg agcttattta tcagatattc taaacggcaa tcgtgatgga aagaaagcac
    16621 aacagcatat cgaaacagtg aaaaaaatat tggacatccg ataggggggt aggcgatgac
    16681 aaaactaaaa aaacaagatt ttgtaaaaaa atacaattat tctccatcta cttatcaacg
    16741 tcgaatgtcg gaactaaaaa atacagcaat tttctcagcg gcgtatgaac gggtcacagg
    16801 acaagaagtt tggatcaata cagaattata cgataaattt ttgtctttca aatcctataa
    16861 caggttacgc acaagaaagg taacacctaa agaatttatc gagaagcatt tagttgattt
    16921 ataaaaaaca gaacattttg agaggtgaag gttaatgggt aaattcaaca gagcattggt
//
LOCUS       DAYYFU010000054        15997 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000054 DAYYFU010000000
VERSION     DAYYFU010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.056"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACHA34_002114"
     CDS             226..894
                     /locus_tag="ACHA34_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFM8792723.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="ACHA34_002115"
     CDS             complement(1094..1480)
                     /locus_tag="ACHA34_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792724.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="ACHA34_002116"
     CDS             complement(1452..2834)
                     /locus_tag="ACHA34_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8792725.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="ACHA34_002117"
     CDS             complement(2990..4177)
                     /locus_tag="ACHA34_002117"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFM8792726.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="ACHA34_002118"
     CDS             complement(4184..5215)
                     /locus_tag="ACHA34_002118"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8792727.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="ACHA34_002119"
     CDS             complement(5231..6685)
                     /locus_tag="ACHA34_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFM8792728.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="ACHA34_002120"
     CDS             6860..7765
                     /locus_tag="ACHA34_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFM8792729.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="ACHA34_002121"
     CDS             7856..8533
                     /locus_tag="ACHA34_002121"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8792730.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8530..8700
                     /locus_tag="ACHA34_002122"
     CDS             8530..8700
                     /locus_tag="ACHA34_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792731.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8814..9710
                     /locus_tag="ACHA34_002123"
     CDS             8814..9710
                     /locus_tag="ACHA34_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8792732.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="ACHA34_002124"
     CDS             complement(9765..11135)
                     /locus_tag="ACHA34_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8792733.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="ACHA34_002125"
     CDS             11373..12119
                     /locus_tag="ACHA34_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8792734.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="ACHA34_002126"
     CDS             12116..12955
                     /locus_tag="ACHA34_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8792735.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="ACHA34_002127"
     CDS             12956..13906
                     /locus_tag="ACHA34_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM8792736.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="ACHA34_002128"
     CDS             14047..14799
                     /locus_tag="ACHA34_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792737.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="ACHA34_002129"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="ACHA34_002129"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFM8792738.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5192 a         3002 c         3221 g         4582 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ctggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccagc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttt
//
LOCUS       DAYYFU010000055        15952 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000055 DAYYFU010000000
VERSION     DAYYFU010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.057"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(388..810)
                     /locus_tag="ACHA34_002130"
     CDS             complement(388..810)
                     /locus_tag="ACHA34_002130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFM8792739.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(824..2230)
                     /gene="atpD"
                     /locus_tag="ACHA34_002131"
     CDS             complement(824..2230)
                     /gene="atpD"
                     /locus_tag="ACHA34_002131"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFM8792740.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2253..3155)
                     /locus_tag="ACHA34_002132"
     CDS             complement(2253..3155)
                     /locus_tag="ACHA34_002132"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFM8792741.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3169..4725)
                     /gene="atpA"
                     /locus_tag="ACHA34_002133"
     CDS             complement(3169..4725)
                     /gene="atpA"
                     /locus_tag="ACHA34_002133"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFM8792742.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4748..5290)
                     /locus_tag="ACHA34_002134"
     CDS             complement(4748..5290)
                     /locus_tag="ACHA34_002134"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFM8792743.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5277..5801)
                     /gene="atpF"
                     /locus_tag="ACHA34_002135"
     CDS             complement(5277..5801)
                     /gene="atpF"
                     /locus_tag="ACHA34_002135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFM8792744.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5892..6107)
                     /gene="atpE"
                     /locus_tag="ACHA34_002136"
     CDS             complement(5892..6107)
                     /gene="atpE"
                     /locus_tag="ACHA34_002136"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFM8792745.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6160..6879)
                     /gene="atpB"
                     /locus_tag="ACHA34_002137"
     CDS             complement(6160..6879)
                     /gene="atpB"
                     /locus_tag="ACHA34_002137"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFM8792746.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7181..7645)
                     /gene="smpB"
                     /locus_tag="ACHA34_002138"
     CDS             complement(7181..7645)
                     /gene="smpB"
                     /locus_tag="ACHA34_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFM8792747.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            complement(7667..10024)
                     /gene="rnr"
                     /locus_tag="ACHA34_002139"
     CDS             complement(7667..10024)
                     /gene="rnr"
                     /locus_tag="ACHA34_002139"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFM8792748.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            complement(10027..10782)
                     /locus_tag="ACHA34_002140"
     CDS             complement(10027..10782)
                     /locus_tag="ACHA34_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8792749.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10885..11121)
                     /gene="secG"
                     /locus_tag="ACHA34_002141"
     CDS             complement(10885..11121)
                     /gene="secG"
                     /locus_tag="ACHA34_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFM8792750.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11239..11517)
                     /locus_tag="ACHA34_002142"
     CDS             complement(11239..11517)
                     /locus_tag="ACHA34_002142"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8792751.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11630..12598)
                     /locus_tag="ACHA34_002143"
     CDS             complement(11630..12598)
                     /locus_tag="ACHA34_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8792752.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12813..12857
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12881..13855
                     /locus_tag="ACHA34_002144"
     CDS             12881..13855
                     /locus_tag="ACHA34_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFM8792753.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13860..14435
                     /locus_tag="ACHA34_002145"
     CDS             13860..14435
                     /locus_tag="ACHA34_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFM8792754.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14502..15641)
                     /locus_tag="ACHA34_002146"
     CDS             complement(14502..15641)
                     /locus_tag="ACHA34_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFM8792755.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4357 a         3443 c         2856 g         5296 t
ORIGIN      
        1 tctgagccag aatgtggttc attctgactc agatttgatt ttgcacaact catcagtcct
       61 tattgacaaa gagcccaaaa gtcttgtcgc agtcccttat tccaaataaa cggtgttcaa
      121 tcatctgacc ctcggtatta gctcaaaaaa cggcaaaata tgaggaacta ttttccattt
      181 gtttctctaa tactcaggac aaaacgctga tttcacaacc tccgatatac ggtgttccag
      241 aagtagcttc ttcgaaaaat aagctgagat ttcacaaaaa tttgagaagc aattttcgta
      301 aattccttct tatgtcttga agctgaccac ttctgtcata acctcttttt actgttctta
      361 aacacggata aacatccgca tctcatttta tgagtgctta gatactttga tacgattgat
      421 tgctcggtgc aacgcaacag ttgcccgttt caattcgttc gtatcttgtt ttgcttttgc
      481 ttcttcgatt tggcgttctg cgcgttgttt tgcacgttcc gcacgagata catcgatatc
      541 acgttcacgt tccgcactat cagcaacgat tgaaacaaca ttatcacgaa cttccatgat
      601 tccgccatta acagcgatcc aatcgacgtg ggtatctgaa tctgttcgtt ttacacgaac
      661 ttcatcaatc gtcaatggaa cgatgattgg tgcatgttta ggcaaaatcc caagctcacc
      721 agctgtcgta cgtgcaacga cgatagctgc atggtgatca tagaccaaac cggcaggagt
      781 taccacattt actgttaaat attgatccat ggggcacccc tttctagtag cctagtgttt
      841 tcgctttttc tacgacgtct tcgattcggc caacacttcg gaaagcttct tcaggaagat
      901 catcgtattt gccttccaag atttcgcgga atcctttcac tgtttcttct acagggacat
      961 atgaacctgg aagaccagta aattgttcag ccacgttaaa gttttgtgac aagaagaatt
     1021 ggactctgcg ggcacgagaa actaacactt tttcttgatc ggataattca tccataccta
     1081 agatagcaat gatatcttgt aattcacgat atcgttgcaa tacgtgttgg acttctgttg
     1141 ctactttata atgttcttct cccacgattt caggtgctaa agcacttgaa gaagaagcca
     1201 atggatctac cgctggataa atcccttgtt ctgttaattt acgttccaag ttggttgtcg
     1261 catccaaatg ggcgaatgct gttgccggcg ccgggtcagt gtagtcatcg gctggcacat
     1321 agattgcttg gatcgatgtg atcgaacctt ttttcgtaga tgtgatacgt tcttgcaatt
     1381 gacccatttc tgttgccaat gttggttgat aaccaaccgc agaaggcatc cgtcctaaca
     1441 aggctgatac ttcagatcca gcttgagtga aacggaaaat gttgtcgata aacaacagta
     1501 catcttgacc ttctacatca cggaaatatt ccgcaatcgt caaaccagtc aaagcaacac
     1561 gcatacgtgc acctggtggt tcgttcattt gtccgaacac catggctgtt ttttcgataa
     1621 ctcctgaatc tttcatttca taatacaagt cattaccttc acgagtacgt tcacctacac
     1681 cggtaaatac agaaatacca ccatgttctt gggcgatatt atggatcagt tcttggatca
     1741 gtacggtttt accaacaccg gcaccaccga atagtccgac tttcccacct tttaaatatg
     1801 gggcaagcaa atcgataact ttgatccctg tttccaaaat ttctgtactt gtacttaatt
     1861 cgtcaaaggc tggtgctttt ttatgaattt cgcttctttc cgcatcttct gggaaaggtg
     1921 tttctaaatc gatcgtatct cctagtacgt tgaatacgcg acctaatgtt tctttaccta
     1981 caggaacaga gattgctttg cctgtatcaa tgacttccat tcctctttgt aaaccatctg
     2041 ttgattccat cgcaatggtc cggatcactc cgtcccctaa ttctaacgct gcttcaagaa
     2101 caacttttga tttattttcg tcatttttat aaacaactaa tgcgttgttg atatctggta
     2161 aggattggtc taaagaaaat tccacgtcga caacgggacc gattacttga acaatcttgc
     2221 ctgaactcat cttttttcct ccaatctcaa tactattcta gtgccgaagc tccggcaaca
     2281 atttcggtaa tttcttgggt aatagcccct tgtcttgcac ggttgtaaga aaccgtcagc
     2341 tcatcaatga tattagcagc attatcagtt gctgttttca ttgctgtcat accagcagca
     2401 tgttctgctg ttttggcgtc aacgatcgca ccgtagatca aactttcagc atactgaggc
     2461 aataactgag acaagatttc ttcttttgaa ggttcaaaaa tatattcttg ttcgaaagta
     2521 gttgcttctt ctggatctaa gtctgagatt ggcagcattt tttctacacg aaactgacta
     2581 gttagcgaat tgatatgatg gttataacat acgtataatt catcgaatac ttcgttttgg
     2641 tacatagttg ttgccatatt tacgattttg cggacttcat caaagcttgg ctgatcagat
     2701 agattgcgca gctcatatgc tagattgata ccgcgtgctt taaagaagtc tgcaccagtt
     2761 ccgccaattg cgatcatcac gtaatcatcg ggtgattggt ggtcttcttc taaaatagac
     2821 atcgtttgtt tcaatatcga gctgttatat ccaccgacta aaccgccatc tgcagtgatg
     2881 acgatataac ccgttttctt aacaggacgg ctgagcaaca tgctgttgta attgatatcc
     2941 cctctaggat tttctgaggc aatatcattt aactgagtag cagtcaaatg cgtaacgatt
     3001 tctcttactt ttgttgcata aacctgaaac ttctgtgaag aagcttcaga ttttgtcaac
     3061 ttagaagcag acaccatctg cattgcccgc gtgatttggc tcgttttttt cgttgaagcg
     3121 atacgtgtct tgatttcatt taatgaagca cccattcatc tcacctcact atgcattttg
     3181 aatggatttc aatttatctt ctgtagagga attatttcct tttgtagcta agaacatgtc
     3241 tttatattcc tgaatcgcac tgttcaacgc ttcttcttct ggaagatctt ttgttgtacg
     3301 gattgtttca aagatatctg aatgattggt atccagatat tcgaataatt catgttcgaa
     3361 gtctaaaata ctgtctacag ggatactatc taagaatcca tgtgtcaaag catacaaaat
     3421 cactacttgt ttttcaacag ctagcggtgc atgcaatttt tgtttcaaga tttctactgt
     3481 acggcgtcca cgatttagtt ttgcttgtgt tgctgcatcc aagtcagaac cgaattgtgt
     3541 aaatgcttct aattcacggt aacttgctaa gtctagacgt aaagtcccag caactttttt
     3601 cattgctttg atttgcgcag aaccccctac acgagataca gatagtccgg catcaaccgc
     3661 tggacgagta cctgcgtaga acaagtcgct ttctaagaag atttgtccat cagtgatcga
     3721 aataacgttt gttgggatat aagcagagat atctccagct tgtgtttcaa caaatggtaa
     3781 ggcagtcatt gaaccgccac ctaaatcatc acttaatttt gctgcacgtt ccaataagcg
     3841 tgagtgtaag tagaaaacat cccctggata agcttcacga cctggtggac ggcgaagcag
     3901 caaggaaagt tcacgataag caacagcttg ttttgaaaga tcatcaaaaa tgatcaagac
     3961 atgtttcccg ttgtacatga actcttcacc cattgcagta ccagcatatg gtgcgatata
     4021 aagcaacggc gctggttgag acgcaccggc attcacaacg attgtgtaat ccattgcgcc
     4081 atattttttc aatgtttcaa cttgtgtacg aactgtagaa tctttttgtc cgattgctac
     4141 atagatacag atcatatctt gacctttttg gttgatgatc gtatcgatcg caatggaagt
     4201 tttccctgtt ttacggtcac cgataactaa ttcacgttgg ccgcgtccga ttggtacaag
     4261 agcgtcgatt gcttttagac ctgtttgcat tggttcgtta acagatttac gttgcataac
     4321 gcctggtgcc attgcttcta ctggacgagc tttgtcagta acgatttcac ctagtccgtc
     4381 aattggctga cctagcggat taactacccg tccaatcaag gcctctccaa ctggaacttc
     4441 catgatcttt cctgttcgtt ttactttatc tccttcacga atggactcga aatcgccaag
     4501 gataatgata cctacatcat ttgattctaa gttttgcgcc attccgtatg agccatttga
     4561 aaattcaagc aactcaccac tcatcgcgtt ttctaaacca tgtgcacgag cgatcccgtc
     4621 acctacataa gttacggtac cgatttcttc gactgaaagc acgttttgat aattttcaat
     4681 ttgttcttta atcaaggcac taatttcttc tgctttgatg gccattcgat tcacctacct
     4741 ctttgtacta tctatttaat tgattacgca tattttctaa ttgcgtgcga atgcttccgt
     4801 cgattactcg atggttagct tcaacaatcg cgccaccaat aatagaagaa tcaacgattt
     4861 gttttaattc gactgattga tagttcattg tttttgcaac attcttttct aattgtattc
     4921 gttgttcgtc tgaaagtgga actgctgttg tgacacttcc caacagcaaa ccttgatgtt
     4981 catcatatcg acgttcatat tcttcgatca tgaacaaaag atcatccatt cggttatacg
     5041 cataaacaac ttctaaaaag tttttaacga ttccatcata gccactaaca agcttgtcca
     5101 tgatttctcg tttttcatga ggttctagac gaacatcact taacatatta cctaatccag
     5161 gtacttgttc atagatttga cgtaagctta cgagatcttg ataaatttct tctgctttat
     5221 ttgaatcgat cgccagttca aataaagctt tcccgtaacg tcttcctact gtatatttat
     5281 ctagtttcat ttgaagaacc tagaccttcg atatattgat tgattaatga ttcgtgcatt
     5341 tctggagata attctttgtt caagattttt tccgcgattt gaagagatag ttctgcaaca
     5401 tcgtctttta cagagttcag cgctgaatca cgttctacag tgatatcagc ttgggcttta
     5461 ctttttagac gagctgcttc ttcttgagca tccttcaaaa tattttggcg gcttaattct
     5521 ccactttctt tcgcattttt gatgatatca gctgcatcag aacgagaggc taacaattgt
     5581 tgttcgcgtt cttgttccat tttcgctgag ttgatgcgag attgttctgc agaatccaaa
     5641 tcattggcga tcttgtcttc acgttttttc aaaatatcgc tgattggtcc ccaagcaaaa
     5701 tgcttcaaga gagccagtag tatcaaaaat gagccgctga caacgatgac atttcctaac
     5761 atcgcattgc caacttctgc aattgccaat tgattcagca taggcgagcc ttcctttctt
     5821 tgaagtactt ctagttttca tgtaaaaaag cagtaacaaa ccaaattgtt tctgccttaa
     5881 tactgattca attaaacacc aaaaactaga atcaaggcga caactacccc taagatagga
     5941 acagcttcga ctagagctac cccgataaac attgttgtac ggaattgacc agacatttca
     6001 ggttggcgag ccattgattc gattgtttta gagataactt gaccgttacc ataaccggct
     6061 ccgattgctg ctcccatgat tgcgattgct gctgcgatat aattcataat tgttttcctc
     6121 ctaaattaga taaatagtag tttgttttta aaacgaatat tattcttcca cttcaatctt
     6181 atggctcata taaaccatag ataaagtaac gaagacaaat gcttggatgc ttccgataaa
     6241 caaggagaat gcgatccaga ccatctctaa tggaattgct aacggcaatg agaaccagcc
     6301 taagcttatt accatttttg caatcaaggt tagcaaaact tcccccgcat aaatattccc
     6361 ataaagacgt aaagcaagtg ttaataagtt tgtaaattct tcaatcagtt tcatcggaaa
     6421 cataaaacta tatggtttca agaacgaatt tacgaaatat cctttgaagc ctaacttttt
     6481 caccccaaat aagtgagtaa gtaagatcat tataaatgcc aaagttaaag taacaaacgg
     6541 gtctgctgtc gggcttttcc aaagtgttgt ttcgttaggt aaaacgattt ttgttactaa
     6601 accgatgttg tttgcaacaa aaacaaataa aaacaatgta aatgccaaca aatggaaatt
     6661 gctcaattct tttctaggca gattatctgt tacgattcct cgactaaaat caatgaccca
     6721 ttctatcgca ttttgtttgc ctgttggttt caacttaaga tttcttgtaa aaaagtaagc
     6781 taagaagaaa acaatcaaac aggtcagtaa caccattata caaactgtac catcaaacca
     6841 aattggtcca atgtggaacg ttagcgagcg ttcatccaat ccatttcacc tcttttcccc
     6901 gaaattcaca taaaatcaat tcatgtgcgt atctggtttt ttccaatcgg tttctaacca
     6961 cacggtatca taccaccatg aaacagaaaa ttcaaaacat tttcatgaaa attagacact
     7021 tttttatttc agtcaaaaac gaagcgaatt tttctttata cacggtgctc ctgatctaag
     7081 aaaaaaccta tgataatcat gttttatcag attaaatgct aacaaatttt atcattaagt
     7141 aaaagcaaaa gcaccgaata tgcgaaaaag gataatgaaa ttatctagag aaatttttta
     7201 atgttcgatc aatttgtcga tcgatatctt ttcgtttcaa atcttcccgt ttatcataag
     7261 attttttccc tttcgccaga ccaatcaata ccttggcata gccgttacga atatagactt
     7321 ttaaaggaac aacagtgatt ccagtatttt tcaattcatt ttcaagccgg atgatttgtt
     7381 ttttgtgtaa taataacttt ctcgtgcgca acggatcatg attaaaaata tttccttgtt
     7441 cataaggact gatatgaaca ttatgcaaga aagcttctcc gttgcggacg cgaataaatc
     7501 catcttttag attgatccgg ctgtttcgta tcgacttgat ctcggttcct tgcaagacca
     7561 tccctgcttc catcgtgtcg atgatcgaat aatcatggcg agctttcttg ttttgtgcaa
     7621 ttaattttcc ctcgcctttt ggcatagctg tttcctccaa ctcattttat tttttctttt
     7681 tcttcttttt aacacctttg taaaatggct tcttcccttt tttcttaccg aacgtttgct
     7741 tactatcgct atatcgtttg ttcttcttgc catttctttc tgggcgtttt cctgcttgtt
     7801 ttccttcgcg ttttcctgat tttccatttc gtgtttttgg ccgttccaac gctgtaacct
     7861 cttctgcgga aacaagttca aaatctactg ctcgttcatc tggatcagct ttgactactt
     7921 ttactcgcac tttctgtccg attttaaaag tcagaccagt tctttcacct acaagagcta
     7981 aatgattttc aacaaaatgg aagtagtcct gtttcaattc attgatatgg atcaatccct
     8041 ctactgtatt cggcagttcg ataaacaggc cgaatttcac aacagagctg atgatcccat
     8101 cgaattcttc ccccactttg tccatcatga attctgcttt cttcatggaa tctacttcac
     8161 gttctgtttc tactgcacga cgttccatct tagaactatg atctgcaatt tcaggaagtt
     8221 ccgtagccca cgaactttga ttgctttcac ttttatcttc actgtaagaa cggatcagac
     8281 gatggacgat tagatcggga tagcgtcgaa taggagatgt aaagtgtgtg tagtaatctg
     8341 ccgctaagcc ataatgccca aaattgtctt ctgaatagcg tgcttgttgc atgcttctta
     8401 gcaacatagt attgataact gcagcttctg gtttgtcttc tactccttcg atcacatgct
     8461 gtaaatcttt tggtgtgatc gttcctttcg ttcctcggac cagaatgccc aaagctgaag
     8521 caaaatcaaa gaagcgctgc attttttctt cttttggctg ttcatggatt cggtaaataa
     8581 acggcagatt taatttatta taatgttcag ctaccgtttc attggccgca agcatgaaag
     8641 attcgatcaa gcgttcacca attcctctgc ttcttagctg gatatcattt gggtgtcctt
     8701 cagcatcaac aaggattttt gcttcgcgat cctcaaaaga aatcgcgcca cgtttgattc
     8761 gcatcgtttc aaggattcga tgcaattcac ccatttcttg gaacataggt acgagctgag
     8821 catagcgttg catcgtttct tcgtcttgat cttctaagat ttgattgaca gctgtgtagg
     8881 tcatccgttc tgttgtttgg atcacacttg ggaaaatatc atgagagact acttgtccat
     8941 ttggatcaat ctccatttcg caactcatgg tcagccttgg aacatgggga ttcagtgaac
     9001 agataccatt cgataaacgt tgcgggatca tcggaatcac tcgatcagtc aaatatacac
     9061 ttgtccctct ttcatatgct tctctgtcca attgactgcc ttgtgtcaca tagtaagaaa
     9121 catctgcaat gtgtactcca agaaaataat taccgttatc taatttttta accgtaactg
     9181 catcatctag atcttttgca tcttctccat caatcgtaac aatgagctga tttcttaaat
     9241 ctctacgtcc agttatatcc ttttcgctga tttgatctgg aacttcatct gcttcagaca
     9301 atacatcatc aggaaatttc gtagggatac cattagccac gacaattgag aggatatcca
     9361 tgccagggtc attcttatga ccaatcactt gcttgattat cccttctaaa ctagtcgcat
     9421 accctttttc agggtaatgg gtcagttcga tcagcacaat acttccgtca actggacgaa
     9481 ctccctctgc tgctgcatat actttaaatt ggttgagctt tctatctttc gggataacaa
     9541 cgccgtaaag atccgtttct tgtatttctt cttctgaata agcaacgaat tcccctgcta
     9601 actgagtagt cgcccggtct cgaatctcga ctactttccc ctctgctccg cgatccatat
     9661 ctgtatttgc tcgcttcatg atatcgattg ctacggtgtc tccatccatt gcaaaaccag
     9721 tcgcttcttt tgggatatag atgtcatctt cttcgggatc aatcgttaca aatccaaatc
     9781 ctcgttcatt tgctcgaaat gttccttcga tcaagattgg ttttaaagga agtttcactt
     9841 ttcctttttt attgaaaact atactctgtt ctctttccat ttgagcaatc gtttgtacta
     9901 gtaatttaaa atcatcgctt ttttggaacc ctaaaccttc agcgatttct tccatagaaa
     9961 aactgcgttt gcgactgctt tccatgaaaa agagaatttt ctcttttatc gtttcttttg
    10021 tcatatttat tcctcattcc aaggtaattt ttctaaaaat tccaagacgt ctttttctaa
    10081 ttcgcgtctt gctgtaccaa ctgtaatcac atgaccactt tcaggatacc atcgaagtgt
    10141 cactcgctga cggctcaact tgcgtgcagt ttcaaatacg ccattagggt cgatcatttc
    10201 atctttccca gcttgagcta aaaatatcgg ctgttggata tctgacaacc tactttctac
    10261 gattgctgcc tgatcttgta tatccattaa ttgttgttca gctaacgagc ggtaagcaac
    10321 tgctttttca ttgatttctt tctctgattt acctgctgtc ttcagcactt gacgtacgta
    10381 tttttcaaag ttttcaggca cgtttgtttt tacaggagac aaaggcgaac agaaaaaacc
    10441 gccgccaatc actgattctt cttctagtgc tcgtacagca aagatgccgc ccattgataa
    10501 tcctaatacg gcaatatcag aaaactgctc agactgtaaa aaatggatgg cttttttagt
    10561 gtctgcccac cagctttctg ctttttcttg caaaatatca tatggctcca ttgttccgtg
    10621 tcctttaaaa agcggcgcat aaactgtata atctgctttt tccaaaaatc gtgcgagcat
    10681 tcgaacatcg tttggacttc ctgagtaagc gtgaagcagc aaaaccgctc gctttccatg
    10741 ttttgcataa aggggttttg gtaattgtct cactcgtctc acctctttcg cctcatttta
    10801 ccatagtttc tttcataact tcttcttaag cgttctcttt gcaataaaaa aagccgcccg
    10861 ccgttttgcg agaggcttat tcaactactt cgaagataaa tatgctagga caaataataa
    10921 gatcatccag atagctccca taactgcagt tgatcgctgc atgacagctt caaagccgcg
    10981 agctttttgt ttgccgaata gttgatccgc accgccggta aatgcactcg ctgcactatt
    11041 ttgcttgctt ggctgcatca taatcgcaat gaccataaca atcgataaaa cgatcacgat
    11101 tcccaaaatt aaattataca tatcgcagcc tccttcccaa aagatatcgt ctctttaact
    11161 ttatcataga taaaagtccg tgacaactct atataaatgg aataacagct gaaagttcaa
    11221 aactttcagc tgttattctt atgactgacg taagacgtct tgatattctg gtttcttttc
    11281 aaactctttt tttgcataag ggcataaagg aatgatcttt ttcccgtctc gtcttgctaa
    11341 ttctactcca gtatacacta gcttttcagc taacttttga ccacgatatg ctggatctac
    11401 aaatgtatgg tcaatgatca tgatgtctgg gcccgcatct gaccatgtca tttctccgat
    11461 ctcttgttgt tcatcattga ataaagcaag acgatttttt tcttctttga tttccatatg
    11521 ccgcactcct caaataactg atattttttt attcctacct tattttagca gtctcctgat
    11581 ctataaacaa ttatttatcc tcgaattact tacaaaaaag aagagaaaat taattcatcg
    11641 ttattctttg ttcaataaat gaaaaaatca ctgtccaata ggttttcgga tctttcatcg
    11701 atgcttccaa gtgttctgct tctggaacaa gcagcagtgc tttatcccct gccgtagcat
    11761 tttgaatcgt gtacgctgct tctgtaggga caaaatgatc tctttcacca tgaatgacca
    11821 ataaaggcag atggttgctg ccaagctgtc tcgtagctga tgcttgcttt aaggagtaac
    11881 cggcgtattt ctgggcataa tgattcgcat atctcatgac cattttttta ggaaagacag
    11941 tgattttact taacatatat cggaattctg cataaacaga aacatatccg ctgtctaaaa
    12001 taaatccttt gaccgctgat ggaagttttt caccacttgc catcatgatc gtagaagctc
    12061 ccattgatcc gccgtgtaaa ataatggacg cattcggctg ttcatctaag atgagttgga
    12121 tccaggcaat taaatctagc cgatctagcc atcccattcc gatgatttct ccttcacttt
    12181 ctccgtgcgc acgtaaatca ggtacaagaa cgttgaatcc tttctctgca tatcttttac
    12241 ctatatgaga catgtctctt ttccctgtgc ttcggtaatc atgtacgcaa atgacccatt
    12301 tattctgctt atttggatgg atgaacatct gaccagctag tttcaacccg tcataacttt
    12361 tgattgtttt gggtgttccc tgctcccaaa agctttcaca ctcttcttct aatttttgtc
    12421 gttttttttc tactgacgtg actgcagcag ggtccatcaa acgatgacca gtgtcttcat
    12481 accagtcata tcctctctgt actcccgctc ttactaattt ctttgcataa aaataagaaa
    12541 gaaaatggta actgatccct ataattatca gaataagaat gaaataaaaa ataaccattg
    12601 tacacccgcc gctttctgcc aaactataat ctaactatta tccaatgtac cgatccatag
    12661 gattctttat tttcggcaca cgtaaattcc atttcttttt cattgtatca tttacttaaa
    12721 gttctcctca atagatttac ttgtaagaga gcatttacat tcgcaataaa agccagtata
    12781 ctaatttcgt gcattataat caagacgctc gtcttggact ggttcggaaa cttcccagaa
    12841 taaaaaacta agtatctcta aagaaaggag attggaaata atgcgaaaag taattattga
    12901 ttgcgatcca gggatcgacg acactttagc tttatcatta gctgtcaaat cccctgatat
    12961 cgaagtagtt gctattaccg tagtttgtgg gaatgtccct gcagatatag gtacgcagaa
    13021 tgtgctgaaa tgcctggaac gctgtgatcg tttagacatt cctgtttatc aaggaatgga
    13081 aaaacctttg aagcaacctt tcatcagtgc acaagacact catggtatgg atggtttagg
    13141 ggaaacaaat ttcccgatga tcttgagaaa acaggcagaa cctctccatg ccgttgattt
    13201 tttggctgat tatttcaaag aaaagacaaa tacatcagtc attgctttgg gtcctttgac
    13261 caatatcgct tttgctttga aagtcaaccc aaatattggg aaacatatgg atcgttttgt
    13321 ctcgatgggt ggaacttaca aaagccatgg taactgttcg ccagttgctg aatacaatta
    13381 ctggtgtgat cctgaagcag cttcttatgt attcgagaat ctcaaacaaa cgatagaaat
    13441 ggttgggtta gatgtgacaa gagaaatcgt cttgacaccg actatcctcg aatactgctg
    13501 tcaaatgaat cctgaagagg gagagtatct aaaagcgatc actcgttttt attttgactt
    13561 ccactggaaa caagaaagaa ttttaggatg tgtcatcaat gatccgttag ctgttgctta
    13621 ttttatcgag ggggccatct gtgaaggttt caaaagcttc actgctgtgg aaactcaagg
    13681 aataagtcgt ggacaaacat tggttgatcg ttatgaattt tggcagaaac ctgctaacag
    13741 taaaattatg acaaaagtag atactcgtct ttttttccga aagtttttaa ccgttctttt
    13801 aaatgcacaa gaagaaacga ttatgaaaga tttggaaagg ttaaagatgg gctaacaaaa
    13861 tgaataaaaa caaaatggca actcgcgtac ttacagtgat ggctttgtgt attgggatca
    13921 attatattgg aggaacgatt gctttatggc ttcgtttgcc aatctatctc gattctatcg
    13981 gaacgatttt tgctggtgca ttgcttgggc ctgtacctgg aatgctgaca ggattgtcca
    14041 gcggtatttt aagtggagtg actacagata ttttttctct gtattattcg ccaatccaaa
    14101 ttctcacagg cttgcttgct ggattgattt tgccaagata tctcggcaaa gataaaaaca
    14161 gcttgaaact gttattactc gctttgattt tgtcggtacc tggaacgatc gtttcttcga
    14221 tcattaccgt tcagttattt ggcgggatca cctcctcagg atccagtatg atcgttcagc
    14281 tattacatgg gttaggttta agtcagacaa caagtgtgat cttggtacag gcaggtacag
    14341 actaccttga tcgtttgttg tctatcgtcg ttgtttcatt agtcatcact atgttgccac
    14401 aacgcttgat atattcaaaa agaaaaatag gctgaataaa cagagctgga ttagcaaaat
    14461 aatgctaatc tggctctgtt ttttcattta ttaaaaaaat cttacgctaa gtaggataaa
    14521 ataattcctt gatatttttc aatcatatcc aaataattat cgatttcaac atactcatct
    14581 acttgatgag gcaaagtaac gacaccaggc ccaaagacga caaagtcgaa ggaatggtta
    14641 gctttcgtaa attcggctgc atccgttgtt gctgccgctc caactaaagg taatggctgg
    14701 ctgaattgtt gctgaatgca atgaatcaaa ggagaatctg gatcagcttt gacaggtatt
    14761 ttgttgtaat cgatcatcaa ttccaaatga tagtctgtct cttgattcaa ttcattgacg
    14821 attgattgca gcaatgcaat gattttatca tttgggtatt ctggaatcga tcgaatgttc
    14881 ccttggagct gcgcatggct tgggatgctg ttgacttgat tcccgccact gatcaatgta
    14941 acattatgga ttgtttttcc taggacagga ttctcgattg tttcagcgag gtgattcatt
    15001 tcagcatttg ctttcgtaat gaattcattt aggtgattga ttgcattata accttggtct
    15061 ggcatagagc tatgcgcttc ttttccgtgc gaggtgactg tatagttgat tgatcccatg
    15121 tgcgtataca tcaaactgta attagttggc tctccgatga tcaaggcatc taagtcatct
    15181 acatagcctg ctttcgtgag ctgttcccca cctagttcac ccacttcttc tccgacagtg
    15241 gctaataatt tgactgttcc gttaaatggt tttcctgact ctttcagttc gatcatggca
    15301 atcaccattg ctgccaagcc actcttcata tctgtagcac cacgtccgta caaacggttc
    15361 ccatggattt ctgctgcaaa gggtgcatag gtccaagacg attcatcacc agccgccacg
    15421 acatccatat gcccagatag gcctaacact tttcctgatt ggcccttttg gtaacgagca
    15481 atcaaattat ctctaccatc tctgtaagaa acaatctctc ctgttatgtc atgctttgcc
    15541 aaaagcttat tcaaataggc tgctacttca ccttcgtttc cattcactga tttaattcgg
    15601 atgatctctt gtaaaatcgc gattttttct tcttttttca tctaatcctc tcccttgcca
    15661 tttcttttta ttgtatttga gtgtgctcga attttcaaat aggagcaacc aaagtaaaaa
    15721 tgaagaaaga gagccacttt atgaaaaata tgctgctact tccctcctat tttatttata
    15781 cgagaggact tgctcataga ttcctattct actgttctat ccttaaaagc tcttatatga
    15841 tgctagttat tgttagcttt cctttttcaa ggatgatctt ttcttgtacc attctttgta
    15901 aacatcatat acagaagcta gttgcaaaaa aaactctcta cctgctgata tt
//
LOCUS       DAYYFU010000056        15591 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000056 DAYYFU010000000
VERSION     DAYYFU010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.058"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            174..524
                     /locus_tag="ACHA34_002147"
     CDS             174..524
                     /locus_tag="ACHA34_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFM8792756.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            623..1153
                     /locus_tag="ACHA34_002148"
     CDS             623..1153
                     /locus_tag="ACHA34_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8792757.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            1307..2458
                     /locus_tag="ACHA34_002149"
     CDS             1307..2458
                     /locus_tag="ACHA34_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8792758.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(2567..4606)
                     /locus_tag="ACHA34_002150"
     CDS             complement(2567..4606)
                     /locus_tag="ACHA34_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8792759.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4664..5221)
                     /locus_tag="ACHA34_002151"
                     /pseudo
     CDS             complement(4664..5221)
                     /locus_tag="ACHA34_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     regulatory      complement(5369..5535)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            complement(5760..5966)
                     /locus_tag="ACHA34_002152"
     CDS             complement(5760..5966)
                     /locus_tag="ACHA34_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792760.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(5982..6596)
                     /locus_tag="ACHA34_002153"
     CDS             complement(5982..6596)
                     /locus_tag="ACHA34_002153"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFM8792761.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(6743..7426)
                     /locus_tag="ACHA34_002154"
     CDS             complement(6743..7426)
                     /locus_tag="ACHA34_002154"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8792762.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(7535..7993)
                     /locus_tag="ACHA34_002155"
     CDS             complement(7535..7993)
                     /locus_tag="ACHA34_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFM8792763.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(8077..9840)
                     /locus_tag="ACHA34_002156"
     CDS             complement(8077..9840)
                     /locus_tag="ACHA34_002156"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFM8792764.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(10061..11521)
                     /locus_tag="ACHA34_002157"
     CDS             complement(10061..11521)
                     /locus_tag="ACHA34_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792765.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(11729..12655)
                     /gene="glsA"
                     /locus_tag="ACHA34_002158"
     CDS             complement(11729..12655)
                     /gene="glsA"
                     /locus_tag="ACHA34_002158"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFM8792766.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(12678..14105)
                     /locus_tag="ACHA34_002159"
     CDS             complement(12678..14105)
                     /locus_tag="ACHA34_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8792767.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(14136..15350)
                     /locus_tag="ACHA34_002160"
     CDS             complement(14136..15350)
                     /locus_tag="ACHA34_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFM8792768.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         4467 a         3109 c         2678 g         5337 t
ORIGIN      
        1 gagtctcttt ttgagttgaa acgaagaaaa agtgttagta gaaaatcgcc ttcgattttc
       61 caccaacaca aaatattata gaacctttgt ttttttgaat aatcgctttt tcttctcttt
      121 ttcttatata ctataagtag taaataattt gaaaaaaaga aaggtgactg attatggcgc
      181 aaaatcgtat ttttacgaca agttttgcaa gcgtttatcc tatgtatgta gcaaaagcgg
      241 agagaaaaga tcgtataaaa gaggaagtag atgaaatcat ttgttggctg acgggttatt
      301 cccaaacaga actagaatat caactacaaa aacaagtgac tttcgaacaa ttttttgcgg
      361 aagcccctgt ccccaatcct tcacgttcct tgattacagg tgttgtttgt ggtgtacgag
      421 tagaagacat cgaggataac ttgatgaaag aaattcgtta tttagataaa atgatcgatg
      481 agctagcaaa aggcaaaaaa atggaaaaga tcctcagaag ttgatttttc cagagatatt
      541 cttctccttc gctcattttg taacatttaa tttggtaaaa tgactagttc acgttaaaat
      601 aagtcaaagg aggttttctt atatgtttga gacgattaaa caaaatattc aaaaatacgc
      661 cttattaaga gctgctgttt atattatttc tgggattgcg attgtgctaa acccttctgc
      721 tgtttttcat tttatcggtt atctgatcac cgcctatctt gttttgcttg ggttgttgaa
      781 tctttttgag gcttaccgtt ttaaaaggaa aacaggttct tggagtatcg gtctgttcag
      841 tggtcttttt ttcctgatct tagctctgat cgtctatgct tttgcacctg caatcgtttc
      901 tatcctgccg attcttcttg gattagctgt tatcatcaac agcttgttcc agttgttctt
      961 cagcatgaat acaaaagcta aaagttggag tgtttatagt agtattctgt tgttgattgg
     1021 cggattcgtc ttattgttca atccattcaa aagtttgatg cttctattcc aaatattcgg
     1081 ctttattttg attttcatgg gcatccagga gattatcaat ttcatcagaa taagaaaaat
     1141 gactatacgt taatcatttt tagttattaa aatagaccgt ataaaaacca acaatccaaa
     1201 aaaatctttt ggattgttgg tttttttgtg agtatatttt cctatttttt caataatgta
     1261 tgttatgcta agttcgggct atattttata caaaggaatg atgagaatgg aatttattgg
     1321 tattttatgc ctgatattga tttcaacaac gattggctca catatctcgc ggagattagg
     1381 tatcccagca gtgattggac aattacttgt tggtgttttg ctaggccaag ctggattgaa
     1441 ttgggtcaaa cctgatattc tagtccatga cttttcggag attggtgtga tcttattaat
     1501 gtttttagct ggtctggaaa gtgatttatc tcttttgaaa aaatatttcc gacctggtat
     1561 gttcgttgct ttgcttggga tcatcttccc ggttgttttt ggctggttga caggcaaagc
     1621 attccaagtt tcgggaaatg aagcgatttt ctttgggatt attcttgcgg caacttctgt
     1681 cagtatttca gtagaagtat taaaagaact aaacgtagtg aatacaaaag aaggatcaac
     1741 aattttgggt gcttctgtgg tagatgacat tttagtcgtt ttagttctaa gtttcagcct
     1801 atcttttcta acgggggaag cttctagcaa tgcttcttta ccgcttgttt tgattgaaca
     1861 gctaatttat tttgttttta ttttcttatt agtcaaatgg attgcgcctt tcctactttc
     1921 atttgcagaa aaagtgtacg caaattcttc tattatcatc gtatcgctag ttatttgttt
     1981 aggaatgtct tacgtagcgg atcttgttgg gcttagctct gtcattggtg ctttttttgc
     2041 tgggatagct gtcagtcaga caaaggttaa agaagaagta taccacaatg tagaggcttt
     2101 aggatatgca gtatttatac cagtattttt tgtcagtgta ggattagaag ttgatttttc
     2161 tgactttagt gaacaactac tctttatttc tatattgacg cttgtagcga ttttgaccaa
     2221 attgcttgga ggttattttg gtgctcagat tgccggtttt tctcaacata gttcattgat
     2281 ggtcggtgct ggtatgattt cgcgtggaga aatggctttg atcattttac aaattggaca
     2341 gcaaagtcaa ttgatcgaac cacagtacta ttcaccttta gttatcgtga ttttattaag
     2401 tacacttatt tctccacttg ttctcaaata ttttacaaaa aaagtttatg aagtgtgagt
     2461 aaatcaaaaa gaagttgtga aagagacatc gtgaaaaccg tttatttgaa aaaaggactg
     2521 tgacgataac gccacagtcc ttttactgta cttaaaaagc aattttttat tgataatttt
     2581 ggttgaggta ttgcaatagt tccgatgctc gtttggttgc tgaatcatca tcttctttat
     2641 tttccaacat gctgaccatc atataacctt ggttatcaag attgaaagca aacaagaaac
     2701 tgttctcttg cccctttaca tcctgttttt ctttgatttc cgctgtacca gttttcgctg
     2761 ccaacggaat ccctaaagca gaaagagaat gtgctgtacc atttacatct tgcacaactt
     2821 ctctcagatc tggcacgatc gtttgtactg ctgtttcgct gatgacattc ttcttatctt
     2881 ttgtctcttt atctgcaatc aatttaggat agacaagtgt gccattgttg gtaaaaacag
     2941 aatacattgc tgcttgctgg ataggagtaa ttagaagttc gccctgtcca tatccagtat
     3001 cagctagcaa gatatctgag ttaaagctat cttcattaga aatttgtgct ggattcatac
     3061 taattggcaa atcaaggtct tcaccaaaaa tgaatttatc taagcctgta cgaaattttt
     3121 tctcacccat tttcaacgtt tcttgtgccg catatatatt atcggaatag atcaaagcag
     3181 tttttaagtc tacttgtgat acatcactac tgacacgcgt tgttttaaaa gatccccaag
     3241 aactgtcttt ttgccattta agcccgttga tcgtcaacac ttcatttgga tcgatagtgc
     3301 cgttgtcgag accgattgct gctgtaatca ttttaaacgt agatccagga gcataacctg
     3361 tcgcaaatcg gctgatgaat ggatgttcag gattttcttc ataagctttg taatcttctt
     3421 gtgagatccc gtttgtcatt ttgtttggat catagcttgg agagctagca agcgcaagaa
     3481 gatcaccggt ttttggcgtt gtcgcaacag ttgatgcagc ttttcctcct agactgtcaa
     3541 aagctatttt ttgtgccttc gcatcgattg tcaatttgat atctttgcca ttttgaactt
     3601 catgttcgat cagaactttt ttctctacac cgtctgcatc tgtaatgctt aatttcccac
     3661 ctgttgtccc acgaagatcc ttatcaaaag ccatctccaa acctgagcga ccgatttttc
     3721 cattactgct taattctgga ttcttatcaa tatcttctgc tgtgatatct cccacgtaac
     3781 caatcagctg agcagctgct tcacccaaag gataatatct gccgtctact tcttgaatgg
     3841 tagctccagc tggaagttct ggcgttgctc catcaatgat tttcaatggg acaaagtaat
     3901 cgggttgtac ccagctttgt gaaatcgcct gattgatagc atcttctgtt aagtcgaagg
     3961 aggaagcaat cgctttgata ttggctgttt tttcgcctcc atctccaagt ttacttggaa
     4021 cgactcctaa ttgttttagt ttgcctgttg ttgctaatgg ttcaccattt cgatctataa
     4081 tattccctct tgctgcttct tgtgtggtca ggcttacttt gtcatttcct tccatttctg
     4141 gaaaaaccaa gttgggttgc cagttgatcg tggtttgacc atcatttctg tctaatgttc
     4201 ctttataaga aagatctttc aattcaccta atgaggtatt catctttgct ttatatgaaa
     4261 aactgtaagt agaatcgtct tttttatcta cttttagatt cgatatctga agtcctttga
     4321 catcagcagc accgtatata ttctggtatt tatctaagat ctctttttca gataatgcac
     4381 ttttatttgc cgcctttttt gatgtcattt ctgcagcttt gttataatct cccttgttca
     4441 aagcttggac aaattgctca actgtctttt ctccagcttc tactgcttgg gtttcttgat
     4501 agtgctggta gtaaaaataa ccgccacccg cagctgctat taagatcact gcgccggcaa
     4561 tataagcgcc tgttcgattt ttgccgtgct tgtcacttct tttcattttt tcctccttct
     4621 cttgcttgtc attcctttat ttcactattt atacctgttt attctaacat atgtctttga
     4681 ggaaatccat accacaatcc agaagcacac tttctggaag gttcgtttgg atactaccaa
     4741 gtaatttccc aaccgctttg ggtagcttga atattgaagt tacgtctttc agctgctgag
     4801 agatagcttt gatcacttgc tgctgccttc tgatcctccc gaagtctcct tctttgtctt
     4861 cccgaaatcg agcatactgg agtaaagtat taccatccat cacttgctct cctttgttta
     4921 tatatacgcc atctaaatct agagattttt ctgcatcgat cttgactcca ccaggaaaca
     4981 gttcattaac gcaatcgata aaatcttgaa atgtgatact tgcataatag ggagcttcaa
     5041 atttgaaatt ttcctttaat gtttgattta ataaatcaac tcccccataa gagtaagctg
     5101 catttatctt attcattccg taacctggaa tatcgacata tgaatctctc atgatagaga
     5161 tcagttttgc ttgttgtgtt ttctggttgt attgtgcaac cagaagtgaa tccgagcgac
     5221 ctccaccaat cgatcttttt tcaccgcatc tcctcctcac acagcaatcc acttgtcagg
     5281 tgaggagcga gtttgtctca tcaaataagt cagtcacaaa aatataaaga aaaggtcaga
     5341 aaccttttcg aacaagaatt ctgacctagc atcatctcat cgtccgagtt ttagcactat
     5401 acaacgtaaa gaaaacattc ttagctattg gaaaaagcct taatccaaca gtttctgttt
     5461 tacccattgg cgactttcga cgtttctggg caacagccta tgtttgtata ggagcctcac
     5521 ctaacaaggt tattactata ttctatttac gagttcatga tagacttttc ctaatgatta
     5581 ggcaatggaa tttgaaatga aatagtgaaa tttaacaatc tttaatattt tttagaaaaa
     5641 aataacaaat aagttgaaag aatcccgttg caaattcttt cagttgcacc aggattcttt
     5701 gataattttt tagttttatg tgttttttta tatgtttttt cacttttcgg aataaatatt
     5761 tatttgttaa agtaattaaa caatccgcta atcgtttgat ctggcatttc tatttcctta
     5821 ccatctaaag taacagtagt aacgacaaaa tggtcttgca atggcggaac ttctaagtac
     5881 tgcccttgca gaaaatcatt cactgtttca tattcttttt tcatttcttt tgaatctgtc
     5941 gatggtttat agcttatttt taacataact agtgctcctt tttattcggg acgtgcaatc
     6001 gtgaaaattt ctcctattgc tgcataatca tttcctgcca aacggcttac agcattcaat
     6061 tttcttggat cgatccttcc tgtcttatca tcatagactg tttcatttaa atgatatttc
     6121 tgtacttcta gcaaaaataa atcagctgtt actttatttt tattccgtat ctcgacatgt
     6181 tcatataagg atgtttcaaa acgaactttt gcttctgtca atgctggtac tgaaatgatt
     6241 actgaatcag ttaaagtcaa gtcagtcagg tacagttcgc tttcctcatt aggcagattg
     6301 gctgctgttt tatttacttc ttctacgttg tcctcatcaa ggatatggat aacagcttct
     6361 cttgtctcaa gaatattctt tgctgtgtct tttactgcat cttgttttct ctggatcgcc
     6421 aaggataaga tgggtcgatt cgaactgacg atattgaagt aactgaaggg tgcgatattc
     6481 actatatctt ttgctgattt agtcgttact actgctactg gtctagggat gactgaacca
     6541 atcagcaact tgtaattttc acgttctgtc aattctgaag gactccgttc aaacatggtt
     6601 tcgccttctt tctataaaat tcgttaaaaa gcagatcttg aagacaaaat gaaaagtaat
     6661 gcttttttca tcttcttaga atctgcttta aactatctat caatttttct tttttgtcaa
     6721 gaatctgtgc cgtgcgtgtt ttttagttat ttgctttact tgcggcttcg acatatcttt
     6781 tagtgatcaa ttgcggacgg gtgataatag atccaactac gactgaaaaa gcacctaatt
     6841 catatgcttt ctctacttgt tcaggagagt cagtatgtcc ttccataatt actgggatat
     6901 cgacttgttc tatgatttga ttcaataaat caaaatcagg tgtctgcgtc tttcgtgaat
     6961 attctgtata gcctgctaaa gtcgtagaca aaagatcaaa accatcttct tgcgcttgaa
     7021 gcgcttcctc aaaagtcgaa atatctgcca tagctaaccg accgtgttcg tgaatcattt
     7081 ggacaagttc agcagttgtt ttttctgtcg gacgtttacg agaagttgca tccagtgcaa
     7141 tgatttctgc ttgcgtctcc aaaagttctt ccacttcttt ttctgtcgca gtaataaaca
     7201 cttctgaccc atcgtaatct cttttgataa tccctattac cggtaaatca acgattcgct
     7261 tgatttcatt gatatcttct gtcgtatttg ctcgaatacc aactgctccg ccttcttttg
     7321 cagccaaagc cattctcccc ataataaaag aactgtgtag cggctcgttt tccaaagctt
     7381 gacaagaaac gacgactccg cctttgattt gttccagtgt atccacaata tctcctccag
     7441 taatgttata taaaaataca gcttcatata aaaatgtaac ccttttctat tcaggagaca
     7501 ataatgagct atttctttca gcagaccttt cttactaaaa gaaaattact tcaaattctt
     7561 cgtatacgac cagcttctct tctgtaaatt ctatgttcaa cgcttcttta taagcatgtt
     7621 taaaaaactc agcatggact ttccgccaat aagctaatgt ccgatcccct tctccttctt
     7681 tccaagcatg ctctttcgtg acttcactaa aaggcaagat ggtcacattt atatttttga
     7741 tgatacaaac tggttcattt tttccattca ggaccacgtc taattgacca acttttggta
     7801 aaggctcatt ctcttgttta tacagatcgt aaccacttga tgtaccagtc tttatctttt
     7861 ttacaactag ttcgctcaac tcgtctgcca ttttttctgt atccccaaat gaaaagaaac
     7921 ctaatttttc tgattgataa ctaggatatt tttcttggaa ctttttgaca tattcttcta
     7981 cttgctgata cataacatcc acccctttgc tttttataat gaaaaaacca tccagatact
     8041 agtataaaca attatctgga tggtgagcat aatgaattat tgaatattca agacacgcag
     8101 actatcttca acgatttgtt ttggtgttaa atgataacgt tcataaagct gccctacagg
     8161 aacgctatcc gtgaattctt tctctgcacc aaaatttaat acgttcattg aagaatcacc
     8221 ataataacga ctgatatttt cgccaaatcc tcctgacaaa ctaccatctt ctaatgtgat
     8281 gactaattcg tgtgtttctt ttaatttatc taatgtttct acgtcatttt ctgtgatcgt
     8341 tcctggattg atcaacgttg cttcgattcc tttttttcta aaagctttta ctgtttcttt
     8401 tcctaattca agaaaaccgc ctaaagctaa aattgctact tttcctccat tatttactgt
     8461 ttgataggct ggaatactat aatcatttgc gatagctgta ccatgttcta caccatgtgt
     8521 aggaattcgg attgctacag gctgcgctgt ttgttcgaac gcccaatcta gcatagagat
     8581 catatcctct tttgttgctg gagcaagata tttgatgttc gggatacttg ataagtaagg
     8641 aatgtcaaat gttccttggt gtgttcggtc actagctgag atcgttccgc caccgactaa
     8701 aataatcact ggattctcgt tgatcgctaa atcatgtgat aattgatcat atgcccgttg
     8761 caagaaagta cttgattgga agacgactgg acgtactccc tgagaagcca aagcagaagc
     8821 gaaagtgata gagtgctgtt cagcgatccc cacgtcaaaa taatgatctg gatgtttttc
     8881 ttggaatgtt ttcagtccaa aggcgcctgg tatcgctgca ttgactgcgg caataggagc
     8941 tgtacttgtt tgcattttct tatccaaata atccatgatg atcatattaa atgtttctcc
     9001 agaagttact tgctttgcct ccccggtgga cagatcaaat ggtacatgcc aatgatattt
     9061 cattttatta tcgattgccg gctgataacc gcgacctttt tccgtatgga cgtgaagtac
     9121 aatcggatga tcaatatctt ttacttcttc aaataaacgg atcaccgttt ccagatcgtt
     9181 tccattatcc acgtaacgat aatcaaatcc cattgcttta aataggttat tgctgctttt
     9241 accgttggtt tgacgaagct cagctaagtt tttatataaa ctgccatgat tctcatcaat
     9301 agacatctca ttatcgttta ctaatacgat tagattggat tgtaaaacac caccattgtt
     9361 caatccttca aaagctaagc caccacttag ggagccgtca ccgataaatg ctaccacatt
     9421 tcctgattct ttcttcagat ctctcgcttt agctaaacct gttgctaagc tgatagatgt
     9481 agatgtgtgc cccactgtaa aatagtcatg tttactttca tgttggttgg aatatccgct
     9541 cacttcacgg taatgtcctt tttcaaaacc attttttcga ccagttaaaa gcttatgacc
     9601 ataagactga tgggaaacat cccaaacgat tttatccttt ggtgaatcaa atacataatg
     9661 aaacgcaatc gttaattcta ctacacctag attcggacca acgtgcccac ctacatcgct
     9721 gtccttttgc aagatttttt ggcgcatctc ttctgccaac tgtttcattt cctcaacaga
     9781 taattttttg acatctgcgg ggctattgat tgcatccaat acatgtaata ctgtactcat
     9841 aatacattct ctcctccgta ctatcttctt ttaatgaaaa tagatttgtt acactttatc
     9901 tattctacta cttagagttg actctaagtc aatagcattt taaaaaaatt tcagagaaag
     9961 cttgttatat aagtagatct tcgcacgaat caggactcaa ataaaaacct tctacagaca
    10021 attaaaaagc tttgtagaag gtctcctaat taaggtctca tcaagaaata tgttttttta
    10081 ataggtgaag ttcctgagca atatgataca aatggatgtt tgcacctttt aatgagattg
    10141 gatagatctt tgtcttttca gtttctaatc cattttgata gctagcagca tcgcacaaaa
    10201 ccactctttt tttcgcacat agacattcat caggcaattc ttctaatgaa cgaaataaaa
    10261 taggatttat ttcagcgatg ccttcttccg catacaaatg aaattgggtt cgataaaaaa
    10321 attgtttgac tgaactttct gaaacaacaa aaatttctat ttcctgtctc cccgcttgtt
    10381 cactgttgat caaagaaaga cttttgacga attttcgaat gaaattttca ttccatctta
    10441 gttggatttg atttttcttt ttccactctt ccaatatttc tacaattttt ccgtacagaa
    10501 gttgttcttt ttctgataga agatagatcc tttccggtaa aaatacttgt ccatctgccc
    10561 acaacgtacg gacaaattgt aaaaaggatt gcttgaaaat aaaattttct gcatcgtcga
    10621 tttcagcgat actaataatc tgtaaaatca attcattcag tgataggtca ttgagaacaa
    10681 aatttttata aacaacttcg aaatcttttc ttaacagctc catgttctca ttggtataat
    10741 ttctactgtt gaacaaagaa aaaatataat agacatcttc ttcttgtaag tctagaattc
    10801 ctttttgctc ttccaatcct tgacagacta attgaaataa cggcttcgtt tctgcgcttt
    10861 tcttatcttc ttctttcagt ccaaggggaa atgcctttct tccgattgcg atctctattc
    10921 ctcgtatgat aaaggacttt tcatcttccg aatatcttct tttaaaaaaa tgagtctcta
    10981 cataatcaat caactcgtta caagcttctt ctacatacgg atttttattt tcatagcctt
    11041 cccaattgac ataacgtact aaagctagca agatgttccg cgagtcaaat tctgggataa
    11101 tgatttcttt atttttcaaa taatcatttt ctttaaaaaa atccaatacg atttttttga
    11161 ttgtatatac tttgctcaaa gaaataaatt caagatcagc cagttctgta ctcgtgaata
    11221 taccattaaa ataataactt aaaaacttta aaaaataaga ttgaccgtac agaaactgtg
    11281 tgagctcaga aagagcaata ccatgtttga atacgagttt atacttatgc ttctctctta
    11341 cttgttcttt tatacaatat tctagacact cggctagact ttctagatcg tgaaggatcg
    11401 ttggtgtagt cacccttaga gaaatagctg tttgttcgat gtctatttct tggaattcaa
    11461 ataattgttc acacagatac acttttctaa atatatttcg ctcgatgtaa ttttccagca
    11521 ttcctgatac acgcctccag tttttcttat gattttattt aatcatatct gtaaatcgta
    11581 tttttgtttc ctttttattt agaaaagccc tttcaaaaac caataaaaaa agaaactggg
    11641 acaaaagtca aacaagctca caatttcagc ctcattgtag gtagctttga gccgtttgga
    11701 cttttacccc agcctctttc aagaatggtt attcaaagat gtcagcgtct agttctttga
    11761 ctacatcttt caacaattgg ataccagctg cgctgttacc aaatggatct agtgccgggc
    11821 tgaagacacc tatgccgtaa cgatttggca cagctgccat caacccgccg cctacaccgc
    11881 tttttgcagg tacaccaaca tggacggaaa actctcctga ttcatcatac aaaccagcag
    11941 ttgtcataat tgatttgacg attgtagcac tttcttcagt aatgatttgt ttgccattcc
    12001 aaggagcaat tcctttattt gccagcacag cagctaattt tgctatgcct gtagcggtga
    12061 cattgaggga gcattgtctg aaataagcat cgagtatctc cggtacgtct ccttcgatca
    12121 tttgattacc tttcatatag taagcaagag agcgattgat atcacctgtt cttgattcag
    12181 aatgatagat ttcttcattc aatgtgatat taggatcatc acagatctct ttcataaatg
    12241 acaagattcg ctcaaatttt tcatctgcat ctttaccggc aatcaacgag gttgttgcaa
    12301 tcgctcctgc attaacaaaa ggattcattg gatgtttgcg atgattgatt tccatattca
    12361 tgatagaatt gaaggcaaaa ccggttggtt ccgtgttgat tgtttgaaag actttttcta
    12421 ttccgttgtc tttgattgct aataacaata cgggaacttt tgacatgctt tcgatggcaa
    12481 agcgcacatc ggcgtctcct ccctcaatca gttgttttgt atccaaatca taaatcgcca
    12541 tacctaattg tttgggatcc acttcagcta atgctggaat gtaatccgcc acttgtcctg
    12601 tttgataata gtggaaattt tttccaatca attgttctaa ttcttttact tccatttgaa
    12661 taaccagctt tcatttttta tttttctgca tgctggaatt tatgacggca tgcgtagatc
    12721 gtaaatggaa taactaagac gataagataa gaaacaatca gaatcgtttc ataaatccca
    12781 tgctgtccag ctgcgatact agcaggtgga acaaatgaga tacaaaaagc aaaaagtgaa
    12841 gtcaaaagac ctaaacaagc aacgattact tttacaactt ttccgcctgg aacctgatag
    12901 gctctttcaa gactgtcatc tttaaaaacc agcttcaaat agcccgcgaa aaacataaag
    12961 tacaacatca ggtagattac aacagtcaat gacatcgctg caagaaatga aagattattc
    13021 cccccgccgc ttaatgtaaa gacggctgcc cagattgttg caacgatccc atttgcgatc
    13081 atcaaatgag caggaacacc gtgtgtgttt tcttttgcaa aatattcagg aagtaatcct
    13141 tcttctgctg ctacgtgaag tgcttttgaa ggagaggtta cccaagaagc aatttctccg
    13201 atgacaccta aacaaacaag aatcgcaaga attttcgcaa tccatgcaag atttggatca
    13261 atgtgtctta ataggaattc taacgattga atcacccctg tattaagaga caaaccatgt
    13321 tgaggtactg tagtagcgac ggtcaatccg ccaaaagtat ctaaaatgat tgccagaata
    13381 accaatacaa acattgctaa tggataatcc tttttagggt tttccatttc atttgcatga
    13441 ctggcagatg tttctacgcc catatatgct aaaataaagg aaacaaaaac aaccagcgta
    13501 ggcaatttcg aaaaatcagg aatcaatgtt ttcatagaca gtatcgtttg tagtggagcg
    13561 cctgaagcga cataatggat ccctaagcca aacaagacta atgctggcaa caagatacca
    13621 gctacaaaac cgatccgagc aatcaatgaa gtgtatttgg ttccttttaa ctgtgagagt
    13681 aaaatagccc agaaaataat caataggatt gccaatttca gatatttatt gttttgtata
    13741 gcaccaatac ccgttgcata ggataatgca ccagttaaga aatacagcat ggtcaagtag
    13801 ccaactgtta tctcaaacca ttggaaaaag attgcggcaa aaccgaatcg ttttcccaat
    13861 gtgcgactga cccaagtata gacaccgcct ttttcccatc ctttcaccgt tgccatctct
    13921 gctgcgcaca atgccacagg gataaaccat agcaaaccgc ctaataacaa gaaaaatact
    13981 aagctgaagc ctgaggtcgc gaatgtggga tattcatata atgaaacgac cattgagatg
    14041 gtcatagcca aaaagccaaa gaacgttaat ttttttgcag gaattttcgt tttgctttgt
    14101 tgcatgaatc ttcctctttt ctatttgaat ttgtttcaat tgagtttgtt gccaaagaag
    14161 aaaaagtcca atccttcttc ttgttcttct tttgtagtag tgatcgttgt aaagtgcatc
    14221 gccgatttta aaatcactgt tgtcagcaca taggcataca cggcagtaat gatgacacca
    14281 atcacttgaa tccctaagaa gtaccagctg cttggattcg ttagtaaatg gctgttagat
    14341 gcaaagatcc ctatcagaat cgtacctgtg aatccgccca tgccatgaac accccacaca
    14401 tcaagtgcat catcccattg tttcttttta cgaaaatcaa cagcagcatg acagacgata
    14461 cccgcaatga tcccaatcag catggcactt tttgcttcta cataaccggc acaaggcgta
    14521 atggctgcca atccagcaac ggatcctgtt agtacgtcga caaaatcaaa gccatttcct
    14581 tttacttttg caatgatcag ccaagtaatc atcgcaaatg caagagcgat gaatgttgaa
    14641 ccaaatgcat tgactgcttg ttcatctgct cgaagtgcac caccggaatt aaaaccgaac
    14701 cagccaaacc aaagcagtcc tgtaccgata gcagctgcca taagactgct atgctgactt
    14761 ctttctccta aaggaatctt tctttgacca agtgtgagga tacatgctaa agagccgaat
    14821 ccagctgtcg tgtggatgac tgctccacca gcgaaatctc taaatcccat ctgatccaaa
    14881 aatccgccac cccatatcca gtggcaaaca gggaaataaa tcagcagatt ccaaaaaata
    14941 acaagtataa tatagccttt taaattcaat ctccctgcaa aagctcctgt catcaaagga
    15001 acggtaatca cgataaacat cagttggtaa agaaagaaca ttaaaaatgg aatcgttgca
    15061 ccatgaaaca tgttggggaa gcttgtaaca tgtctcatca aaaagaaatc tgccgggttt
    15121 ccaataacac cgccgatatc ccgtccaaag gccaatccga atccaccaaa tacccacata
    15181 atcgttgtta tcccaacgga gagaaaagat tgagacatga tcgttagtat attattcttc
    15241 cgtgctaatc cgccgtaaaa aaaagctaga ccaggagtca tcaggcaaac cattgctgta
    15301 cataaaatca tgaatgctgt atctcctgta ttgataggta atgtattcat ttttttcgcc
    15361 tcctttctac gattacgagt ataaaagaag gtgtagttgt ttttttcttc tgtttttttg
    15421 aattaatact tgcatttata aaagacagac aattaataaa agcttgatgt gttgtgctct
    15481 ttttcgtttt tcacacaaat aaagacaaaa aataaacggt cttcttttta tagaaaaccg
    15541 tttattttcg tcttttttta tttttaaaga tattttttca taaggaataa a
//
LOCUS       DAYYFU010000057        15230 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000057 DAYYFU010000000
VERSION     DAYYFU010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.059"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..787
                     /locus_tag="ACHA34_002161"
     CDS             278..787
                     /locus_tag="ACHA34_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8792769.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ACHA34_002162"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ACHA34_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8792770.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="ACHA34_002163"
     CDS             2322..3302
                     /locus_tag="ACHA34_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8792771.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="ACHA34_002164"
     CDS             3424..4842
                     /locus_tag="ACHA34_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM8792772.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="ACHA34_002165"
     CDS             4913..6382
                     /locus_tag="ACHA34_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8792773.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="ACHA34_002166"
     CDS             6392..6859
                     /locus_tag="ACHA34_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8792774.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /gene="scrK"
                     /locus_tag="ACHA34_002167"
     CDS             6895..7767
                     /gene="scrK"
                     /locus_tag="ACHA34_002167"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8792775.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="ACHA34_002168"
     CDS             7848..10382
                     /locus_tag="ACHA34_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8792776.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="ACHA34_002169"
     CDS             10538..11311
                     /locus_tag="ACHA34_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8792777.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="ACHA34_002170"
     CDS             11304..12095
                     /locus_tag="ACHA34_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8792778.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="ACHA34_002171"
     CDS             12105..12596
                     /locus_tag="ACHA34_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8792779.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="ACHA34_002172"
     CDS             12614..13024
                     /locus_tag="ACHA34_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8792780.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="ACHA34_002173"
     CDS             13028..14428
                     /locus_tag="ACHA34_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFM8792781.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="ACHA34_002174"
     CDS             14451..14825
                     /locus_tag="ACHA34_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8792782.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5286 a         2135 c         3046 g         4763 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactcctag
//
LOCUS       DAYYFU010000058        15072 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000058 DAYYFU010000000
VERSION     DAYYFU010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.060"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..170
                     /locus_tag="ACHA34_002175"
     CDS             <1..170
                     /locus_tag="ACHA34_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8792783.1"
                     /translation="YQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRAR
                     IYLQQGLIFEN"
     gene            complement(553..1281)
                     /locus_tag="ACHA34_002176"
     CDS             complement(553..1281)
                     /locus_tag="ACHA34_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8792784.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1259..2833)
                     /locus_tag="ACHA34_002177"
     CDS             complement(1259..2833)
                     /locus_tag="ACHA34_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8792785.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            3062..4000
                     /locus_tag="ACHA34_002178"
     CDS             3062..4000
                     /locus_tag="ACHA34_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8792786.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            4012..4923
                     /locus_tag="ACHA34_002179"
     CDS             4012..4923
                     /locus_tag="ACHA34_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8792787.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4947..6419
                     /locus_tag="ACHA34_002180"
     CDS             4947..6419
                     /locus_tag="ACHA34_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8792788.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6470..7081
                     /locus_tag="ACHA34_002181"
     CDS             6470..7081
                     /locus_tag="ACHA34_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8792789.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            7111..9024
                     /locus_tag="ACHA34_002182"
     CDS             7111..9024
                     /locus_tag="ACHA34_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFM8792790.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            9152..12133
                     /locus_tag="ACHA34_002183"
     CDS             9152..12133
                     /locus_tag="ACHA34_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFM8792791.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            12130..13791
                     /locus_tag="ACHA34_002184"
     CDS             12130..13791
                     /locus_tag="ACHA34_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFM8792792.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13831..14763
                     /locus_tag="ACHA34_002185"
     CDS             13831..14763
                     /locus_tag="ACHA34_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFM8792793.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4894 a         2389 c         3030 g         4759 t
ORIGIN      
        1 gctaccaatc gggtatttca aatgccttta aggtgtccca ctcaaacggc gtcactgagg
       61 ggctgaataa caagatcaag ctgattaaac gaattgcgtt tggttatcgc aacttttata
      121 actttcgggc acggatctat ttacaacaag gtctgatctt tgaaaactaa aaaaatatgc
      181 ggcgcacaga acgcgccgca tatcggacta agttttattc aaatttgtct tcaccaacac
      241 gatttgacga agagcctatt taaataacta catccattta ttctatcttc taaaatagcc
      301 tttttcattt cagttaaata gttcagcgcc atttttgcac tcgacttgca tagtagtttt
      361 ttccatattc ctcaacctca gcgggggttg aagacataag gaaaatccta tgcgttatgc
      421 tcaactggat ctgattaaat attctaatgt atttttctct accatcatta aatggttaat
      481 agccaaaaat caccactttt caatggctcc ttgttgtata agtataacta gaagatgaac
      541 acgtctgaga aattatagaa gctttttacg gtaattactg gggctgtgtc cagtagtttt
      601 tttgaagaga acagaaaaat agctcacgtg atctattcct actttttgac taatttcatc
      661 aatagataag aatgtgtctt tcagtagagc acttgctctt ttgattcgaa agtctagcaa
      721 gtaatcatga aaggaataac ctgtttcttt tttgaaggcc cgtcctaaat aagcgggatg
      781 gtaatggaat tgtgtagcta catctttgag cgtaagcttt tcactataat tgttttgaac
      841 gtaatcaatc acttgttgaa ttgactcatt aaaagaatca tcagttagag agttttgatt
      901 agctgctgat ttacgcattt tttcttcttg tactaactta actaaataag gaaaatcaat
      961 gggtttcgtt atgtaacttt taatagataa atcaatcccc ttttttgcat actgaaaatt
     1021 ttggtgggca ctaatgacaa tactagtaaa gtagcgattg gatgtttgtt gcaaagattc
     1081 gattaattcg agtccagaaa ttttccccat cgtaatatct gtgataagta aatcaaagcg
     1141 ctggttttgc aggtttttaa atgcttcgtc tccgtctgtg tagcagtgat cgataatgat
     1201 gtcggggact ttcttcaaaa gataatgcct tatgccttct acaattgtac aatcatcgtc
     1261 aacaattgca attctcaaca tgcatattcc cccctttttg tattttgata gtaatcgttg
     1321 ttccgacgtg tagttggctt tctataacaa aacgtgtatc tccaggatag agatgcttta
     1381 tacgtcgaaa agaatttgag atgccgatat tctcactata aggaatatca tcattaagtt
     1441 tttgttgaat atgctccagc ttgtcttggt ccattccttt cccattttca acgattttta
     1501 acacataatg gttggttgtt tcagttgcat agacagtcaa acgattttcc ttttttgatg
     1561 aatcgaagcc gtgtttaata taattttcaa caaatggctg taaagaaagt tttggtaaca
     1621 ttaattgatc aatatgatca atcagctgat agtcaaagtt atgtcgataa cgacgttgat
     1681 gtagttgcaa ggagaggtcg atattaatta attcctcttt caaatcaatt atgtcgggct
     1741 gatatgtatt attgcgatac aacttgctca ataaatatat actgtcgctg atttcttcgt
     1801 catcattcaa tatggcagtc attcgaataa attccaaatt attaaataga aaatgaggct
     1861 cgatttgata ttgaagtgct gcatactttg cttcttgctt caaccaatta ttttcataaa
     1921 gtgttttttg atatgtagca ttatctaaat aaaactgatt gatatgcata gcgatttctt
     1981 tcaaatcacc gactttgttt tcgatattga ttgacttgta gtctcctgta gatgaatgat
     2041 ttaaggaagt aatgatttcc tgatactgct ggatatagtt acgaaaactt ctatttaata
     2101 attgatgaat agcaagaata aacaaactca ttaataagaa aatcatgatt gccagaccta
     2161 ataaggtttg tgacgaataa tttggatttt catatacttg taatgtgtgg ttaccgatag
     2221 ctatctgttg cgtttttttc catttagaag gcgagatgtc ttcagataaa ttgacggaat
     2281 caataccatt agacaaagaa taattgtaat gtgttggaaa gagactcttt tttagcgcgt
     2341 tgatcgaaac gtctagaatc aatgtagcat cgggcatgtc ctcactaatg aaacttaatg
     2401 aacgaataaa agaaattttt tcgttttttg taggtgcgta ggcacttaag tatttttcgt
     2461 aatctaatga gatagatata tttttaactt catcatcttg cacaagtttt tttaagttat
     2521 ctactaaata ggtaggggag cgatcctttg acaaaatatc ttccatgtat tcagcaggtg
     2581 atgagaggta gtagtcatta aggttttctt ggaaatcata attttgatag atctctgtca
     2641 cgataccgga gagaatacga ttatatgttt ccatatagtg ggtgatttgc tgaatgtttt
     2701 gcaggttttg atttttttct ttttcaatca cattgatata ggaaaagaac aatgtgaaac
     2761 aggcaagtaa agtaataaaa aaaatcaata tgtatgtaaa acgcataata acttttgata
     2821 aatcgttttt cattcaaagt cctcattttg ataatctcta attttatgaa tcattatagc
     2881 acatattaca agtgatgtaa acgctttgca aacagggcta cttatgattg tgaatacagt
     2941 ttccgtttaa aaactattct attgtagagg tttaataaac taaaaataat gtataaaaaa
     3001 ttaatgtaag cgattaacca agtgggtata atcaaggaaa cgctgagaga ggagagacaa
     3061 aatgaagcta aagaaagggt tattaggaaa tctaaaaaaa aaccgagcat tgtttattat
     3121 gcttttacca ggtgcgttat ggttatttat attttgctat ataccaatgt ttggagttgt
     3181 aatcgctttt aaagatttca gaatttcatc aaggggattc tttacaagtt tgttcaacag
     3241 tccttgggtt ggctgggaaa atttcaaata tctctttgct tcgcaagatg cttggatcat
     3301 tactcgcaat acggttgttt ataacgttgt aggtattttg ctaagtactt tttgtacagt
     3361 gtcattagca atcattctga gccaaatggc gaacaaacgg ttattgaaaa agatgcagac
     3421 atttatgatt tttcctaatt ttttatcatg ggtagtcatc agttactttg tttacgcatt
     3481 tttgagtacg gataaaggat taattaatat tttactaagc aattttggtg tggaacccat
     3541 aaattggtat agtgaaccga agtactggcc ttttattatt gtttttacga gtttatggaa
     3601 aggcgtaggc tatggatcca tcatttatta cgcgtcaatt atggggatcg atactagtta
     3661 ttatgaagct gctttagttg atggtgccag taaatggcaa cagataagga atgtagtctt
     3721 gccacattta agatcgatta ttgtcattat gttgattttg tctgttggta gtatttttcg
     3781 agctgatttt ggattgtttt atcaattgcc aagaaattcg ggggctcttt atgatgtgac
     3841 gaatgtatta gatacttatg tctaccgtgg attaatgaat agcggagata tcggaatgtc
     3901 tacggcagcg ggattatatc agtcatcagt tggctttgtc ttgattttga ttactaataa
     3961 gatagtcaac aagttagatc ctagttcagc actattttag gaggaatcaa aatgatatca
     4021 aaattaaaac ggcagatgta cttttcgctg ccagtcgaac ggtttttcac aatattacta
     4081 atcttattta ctcttagctg tatttttccc tttattttcg taatcgtcat ttcattaaca
     4141 gatgaaaaag cattacaact caacggttac gaattattgc cggctcaatg gagcactgat
     4201 gcatatcaat acttaataca agatggcgga cagttacttc ggtcattagg cgttacaatt
     4261 atgattacag tgattggaac gttgattact gtttttatga cgggaaccta tgcttatgtt
     4321 ttatcaagag cctctttccc ttaccggaag ttctttactt tttatctttt ttttaccatg
     4381 ttatttgcgg gagggatggt tccttcttac ttagtgatga cgaagatgct tggcttaaaa
     4441 aatacaattt gggctttgat tctacctctt gcttttagcc catacaatgt cattatttta
     4501 aggacattct ttaagaagtc gattccagaa tcgattatcg aatccgctaa aatggatggc
     4561 tgtagtgagt ttcgagtttt tttccaaatc gttttgcctt tagcgatccc aggggttgca
     4621 acaataggtt tattttcaag cttaggttat tggaatgact ggttcaacgc attgctgtat
     4681 attgacacca acaaattgat tccattgcaa tatttgttaa tgaagattca aaattcaatg
     4741 gaattcttag caaataataa tgacattaca ttagcgcagc aacaagcaat tcaaaatagc
     4801 cttcctcaag aatcaacacg aatggcaatg gtcgtagtag ctactttacc gattgcaatt
     4861 gtttatccgt tcttccaaaa atactttgtt caaggattga cgatcggtgg agtgaaagaa
     4921 taaaaaagaa taggagatgt taaaagatga aaaagaacat gcagaaatat ttgattggtg
     4981 gtgcttctgt actaaccctt ggcctattag gtgcttgtgg tagcagtaat ggcgaagcaa
     5041 ataatgaaac aggaaccaca aaagttactt ggtatcaggt tggagaagaa cccaaagatt
     5101 ttgatgaggt catgacaaaa gtcaacgaaa aattaaagga agaaatcaat gtagagttgg
     5161 atatgcggct gattccggac ggggattatc agcaaaagct tggtgtgatg atcaattcag
     5221 gagaagaata tgatatttgt ttcgtcaatg gcacagatta tgtgaattat gggaacaaag
     5281 gtgctttcat tagtctcaac gatcatttgg ataaagagtt gaaagattat gcagctgaat
     5341 tgaatgaagg atttattgaa ggtggggcaa ttaatggcga aacttatgga ttgccagtca
     5401 ataacgatat tgccacaacg gaatattgga cctttaataa agatttggta gatcaatacc
     5461 aattggatgt agagaatgtt gaaggtgtag aaacattggc tccagtatta gaaaagtttg
     5521 cagaaaaaaa tactgatacg aatattacgc caatcggtat gacccgtacc agtgtctgta
     5581 ttccttatga caatattatc ggtggtggtg taccgctagc ggttgcattt gaaggcgata
     5641 cacaaacagt tgttaatact tatgagactc cagaaatgat gacttatttg aaggctatgc
     5701 atgagtttta taaaaaagga tatattttga aagatgcagc cactgttacg acagatccat
     5761 ttcgaattga tagtaaaaac tggtttttag gtcgtaactc aagtggttat caaccattag
     5821 cacaaaaaaa attagaagct ctagcgggac atggattaat tttaaaacca gctggaaaag
     5881 cttttaaaac gactggattt gcaagagtgg caatgcatgc ggtatctgct tcaagtaaac
     5941 atccggaagc agccttgaaa ttgttgaatt atgtgaatac caatcaagaa gtagcggatt
     6001 taatggttta tggaattgaa ggaaaacact atgatcgcaa tgaagatggt tcgattactt
     6061 taacaaactc tgatgattat tatcaaggcg cttgggcatt ggtaaatact tatctagttt
     6121 caaaaacttc accgattaca gaagaaagta aaaaactaga agaagaaagt aaaaagtttc
     6181 aaaatgaagc accgatgtct cctattttag gtttctcttt tgacgcttct gattttcgaa
     6241 cagaaattgc aagtgtacaa aatatcgtgt cacaatatga agtactttta agctcgggaa
     6301 cagttgaccc tgaacaatat gtaccagagt tgttagaaaa actagaaaaa gctggattgg
     6361 ataaaattca ggcatctgct caagaacaat acgatgaatg gcgaaaatct aacaaataat
     6421 tttttactaa atgaaaagaa tagactaagc agttagtggg gggattcaaa tgaacgaaaa
     6481 caaatggacc aaaggattta attgggtttt tcaattgttg ttactaaact ttacttattt
     6541 tatactaatt tgtttaggat ttggaatttt aggttttttc cctgctacga tagctttgat
     6601 tgctggcgtt gtgaatcgaa aaaaatatgg cttgcaagag tctgtgaagt tcatgagtaa
     6661 aatctataag aaaaattttg ttagatataa tctatattgc tggtcagcaa ctttgttgtt
     6721 ggccggcaat attactacga tctggctttg ttggcagcaa agcgcaccat tgttttattt
     6781 attaagtaca ttattatcgg taattacact gttcctagtc tttgcaatgt tgaattgggt
     6841 cgtgatcaaa agtcttgggt tgtggatgat gggagaccga ctgattgact atttgctttt
     6901 cacagcaacg atgttgccaa cggtagtcat gcaaggcttg gtgcttggaa tttatttccg
     6961 aatattgatg attttgcctg tgatttttct gtttggcgga acagctgtaa cggtaatatt
     7021 gattagtaaa ttaactgaaa gagcgttagc aaagttgcct tgtcagatac aatcgcacta
     7081 attttttcac aaaaaggagt atttctttct atgaagttaa ctgataaaca agtagctaat
     7141 atagccttta tccagcaact tgagattcca ttgacacaaa aagatgaaga acaaatagac
     7201 ttgattttta ctgacacaaa ggctaatgtg cgacaaatca agattactaa aaatgagcag
     7261 caacgaattt gcttgtctta ttcacatgaa gacttgctgt atcgaggatt gtttatctgc
     7321 cttccacgtt ttttagctca aggaggagag atgccataca ttgaagaaac ctcgatcatt
     7381 gagaccagtt attcattaga tgcttctcgt aatggcgtga tgaataaaga gactctaaaa
     7441 aaattcattt cttacttagc gttgatgggg tacaacacat tgtatattta cacagaagat
     7501 acctatgaag tagaagacct tccatatttt ggttaccttc gcggagcgta tacaaaagaa
     7561 gaagtgaatg aaattgttga atttgcttct ggttttggca tagaagtagt tccatcgatc
     7621 cagacccttg ctcacttgac ccaatttttg aagtggtatg ctcaacaaga actgatggat
     7681 gatcaaaata ctctactgat cggtgaagaa aaggtttatg cggcaatcga aaagatgatt
     7741 attgcctgca aagaaatgta tcagacagag cgtattcatt tgggaatgga tgaagcctat
     7801 caagcaggtc tgggacgata tttaagcctg tatgggtatt gtgatcggac aactttaatg
     7861 attcaacata tggaccgtgt gttatcaatt gttgataaat ataagatgaa accaatgatt
     7921 tggagtgatt tcatttataa gctattagat gaaaccggta aagataggta ttacaatcct
     7981 gattttaaac tcaaagcatt agcagctaaa ggattaccag caggattaac ctatgttcat
     8041 tgggactatg gttcagatga agtttcctcc tatgagaaag taattgataa ccatttgaaa
     8101 ttttgtgaca ttgatcatta cgttttagct agtggtgccc atatctgggg gagactagcc
     8161 ccgaatcatg ggaaaacaga agcaattgtt tctgcagctg taaaagcttc taaaaataaa
     8221 ggtgtacgtt ctgtgatgtt aactacttgg ggagatgaag ggcaggaaac agagcattgg
     8281 cattccttaa ttggggcatt aaaattagca gattatgtct attctgaaga agagtggaat
     8341 gattttgagg aaatctacga tcgtttatta ggaacaggta gttatcaatt aatgtccgaa
     8401 ttacgatatt tcgatgaagt ggataccatt acaaataaca acttacaaat ggcttccgtg
     8461 agtaaactgc tactttggca agatccgtta gttggtatat atgactttca tgtagccgct
     8521 cataacaaaa ttgccactag aacaattggt acttattatc atgagttagc tcagaaatta
     8581 gatatgaata aaaattggaa tccaaaacaa ccaattttaa caactattcg tcaacgatat
     8641 atacttttag ctgaaatttt agcacaaaag gcagaactag gcatctattt acaacactgc
     8701 tgtctggtag aagaccttgc aggattaatg gaattagcga atcggtgtga agcactagcc
     8761 aatcgagtaa atgaattggc atctattcat gaaataattt ggtatcaaac atacaaaacc
     8821 tatggttggg aagtattaga acaacgatat gctgcattag ctcatcgtct gaaaaccgtt
     8881 gcgcgtcgta ttaaaaatca tcttcagggg caagatgccc tagaggagct tttggaagaa
     8941 cgattaccgt tttgtcaata tcaagtgccg cttgaaattt ctggctttaa ttatcggcaa
     9001 agctcggtgt caggctacaa ttaacgaagg aatcttcccg ttgaacggac cacttgctca
     9061 tctaatggga aagtggttcg ttttctattc ataagtagct taccactaac tcttttatta
     9121 agaagagaaa aaaagtagga agtgagaaaa tatgttatcg gactattatc aaaatctgtc
     9181 agttataagt gtcaatacag aaccattacg taattattat attccttatg caagtaaaga
     9241 agaagcaaaa caagagttag aaagggaaaa aacgagccag tttatcgatc tcaatggtac
     9301 atgggatttt cagtattatc cttctgtcca acagttaccg gaaaattttt ttcaagaggc
     9361 ctttactgaa agctatgata gaatttctgt tcctggagtt tggcaaacag agggatttga
     9421 cttacctcag tatatcaatg tatcttatcc aattcctttt gatccgcctt atgtacctaa
     9481 tgatacccct tgtggtgtat atcggcgtca gtttcaaatt gagcaaacag atgcacgctt
     9541 ttacttaaat ctagaaggag cagatagtaa ttgttttgtt tggataaata gaaactttgt
     9601 aggctatgac caagtgagtc atagtacatc tgaatttgat gtgaccaagt atttacaggt
     9661 tggggttaat gaaattcaaa ttctagtgat gaagtggtgt gatggtacct actttgaaga
     9721 tcaggataag tggcgaatga gcggtctatt tcgtccagtg tatcttcttc aacgaccaac
     9781 tatgcatctg acaagttatc aactacggac gttgactgat cctaaaaaag gaagcggaac
     9841 attaatggtt tcttttcaac gatttactcc tattgaagta tcgttaccta tttcttacga
     9901 attattccat cctgatggac gtctcatagc gaaaggggag tgttactcgg aacaacaaat
     9961 aaatttggat gttgaagatt gccttttttg gacagcagaa acgcctaaat tatatacatt
    10021 acttttaaca tgtaatggtg aatattttaa agaaaaagtt ggctttcgag cactgacgat
    10081 caagaatcgt aagttattgc tgaacggaac acctattaaa ttatatggag tcaactatca
    10141 tgacgtgaat cagcaaggca cgccagtgat gacccgagag cagttacgtg cagatttgtt
    10201 attaatgaaa aaattaaatg tgaatactgt acgaacggca cattacccta aatcaccaga
    10261 tttttatgaa ttggcggatg agattggtct gtatgtaatc aatgaagcag atttggagtg
    10321 tcatggtgtg gttaatctat atgatactga agcgaattat caactaattg ctaatgatcc
    10381 gacattcgaa aagccgatta tggaccgagt gatccgttct attcttcctt atcaaaatcg
    10441 aagctctatc attatgtggt ctatggggaa tgaatctggt tatggagtaa attttgaaaa
    10501 agctcaagct ttagcacgga aattggatga ttcacggttg attcataatg aacgctcgat
    10561 tgaaccagtt attgaccaag tgaatgattt tagtaacttg gatgttatta gtcaaatgta
    10621 tcccccactg gaagatgttc atcgtcattg tcaaagaagt gatactgaaa aacctttttt
    10681 tatgtgtgaa tatagccacg ctatgggcaa cggtcctgga gatcttaaaa cgtacttcga
    10741 tttgattcaa cagtatgatt cctttcttgg cggttgtgtc tgggagtgga ctgatcatgc
    10801 aatcaataga ggaactttag aaacgccatc ttatttatac ggtggtgatt ttggtgaaaa
    10861 atatcacgac ggcaattttt gcgtagatgg tttattgacc ccagatcggc aattaacagt
    10921 aggtgcattg gagtaccgta atattcattc gcctattcaa ctgattgctg tagatgagga
    10981 gctgcaagaa gtaaccttga gcaatataac tgcttttaca gccggttcag catttaacct
    11041 ttctcttgaa tggtatatag atggaaaaaa aacagtttat gaagtaatga atcctttcat
    11101 acttgagcct ggcaaaagtt gttcattgcc catcacagta cctacaacga ttcatggtgc
    11161 tcatacggtt gctgtttcgc tatctgttta taaaaatgat gagctttatg gacagcaaca
    11221 gctctttctt aaaaaagaaa aaatgagatt gccaagaact gctcatcata atgatgcacc
    11281 acaaatttca gtaaaaggca cagagctgat aatcaaagga gaatccttta tttatatata
    11341 tcaattagca aaagctgcgt tcacttctgt aaagatcaat caaaaggaac ttttggaacg
    11401 tcctactcaa ttttcaattt ggcgtgcacc aacggataat gatcgtaact tagtaaacaa
    11461 atggaaagca gctggatatg ctgaaatgtc tttagaaatc agaaaaaaag actggaaatt
    11521 cgtagatgat catctcctga tcgaaacgtc ttatcgagga gttattccag gattacagcc
    11581 aatttttaca atgcacatac agttgaaaat aaatggtgca ggcgtaatca accaaaaagt
    11641 atcagtggag aaaaatccga tttttccaga tttccctcgc tttgggatgg actggccgtt
    11701 gaatgatact ttttcgaagg tttcttattg tggctatggg ccgtatgaaa gttatcctga
    11761 taagcactat gctgcgatgt ttggtattta tgagatggag attgaggaat ttgatcagcc
    11821 atatatcaaa ccacaagaat atggtaaccg ctggcaatgt gattgggtat caatcaaaaa
    11881 tcatcaagga tctaccatga cggtttatgg aaactctaat ttttctgctc tgccttatag
    11941 tcaagctcaa ttaaccgaaa agtcacacca ttatgaatta ataaaagaaa atagaacttg
    12001 gttcaattta agttataaac aaaatggtat cggctcacac agttgcggtc cttatttaga
    12061 agatcgtttc cgttttatgg aagatacttt tgactggaat atagtgatta ctttcaaagg
    12121 agaaaataga tgaaagaaac aaagatcaat caagtcaaaa aaaaattatc tcgttatcaa
    12181 gaagttgttt atcgaaacca ttgcttaaaa tggtcaaaaa ataagctgaa tgttcgtttt
    12241 aatggattat tttatcgttt cgaacagtta gaagaagtct ggaaaccggt aacgaatttc
    12301 caatcatgtc ctgtacagct cataaatgat ccaaaagaaa catacgattt atgtataaca
    12361 cacctttata accagcattt gaaaaatgac ggattttatt taaaggttac taatgacaat
    12421 caacttacga ttgaatcagc aaaccttcga gggtttcaat atgctatgga agccttatta
    12481 gagagttttt tttcggtagg agatcaatta ttattaccgg aattgattct gaaacatgaa
    12541 ccatcagtga aaattcgagg aatcattgaa gggttttatg gtatcccctg gacacatgaa
    12601 atgcgcctct ctcttttttc atatatgaaa gataaccaat tgaatacttt tatgtatgcc
    12661 cctaaagatg atgaattatt acgtaaaaaa tggcgtgaac tctacgatgc gcaagaatta
    12721 gacaaattta aggagctttt atctgctgct gaggctagca atatcgattt ttggtactta
    12781 attagtcctg gaaatgatat tgatattacg tgtgaagaag acattcaagt tcttctgaaa
    12841 aagttagaac aactgattga gcttggcgta tttcaatttg gtctattaat ggatgatatt
    12901 gactatcaat taaaaggtgc tgctaaacgc cgttttcgag agccagcatt cgctcatgcc
    12961 tatttagtga atcgagtaga ggaatatctt agcactcggt tgtccaatca tgaattagtc
    13021 atttgtccta cagaatatga taatcgtcat ggttctcgct atttagcaac gttaagtcaa
    13081 gaaataccag aacaattacc gttcttttgg actggcccaa gcacgttagc ggcgtcaatt
    13141 tctacagaag agctgcagga aatggcttct gtctatcaac ggccgatgct gatttgggat
    13201 aatattccgg taaatgatta tttagaggat aaagaactat tgttcatgag tccatatgag
    13261 aatcgaacac cgaatctttc taaggagaga taccaggtta caggagtagt ttcgaatcca
    13321 atggctcaat tagaagcttc aaagtttacg atcaatagta tggctaatta cttatggaac
    13381 tgtgagcgtt ttgatccttt ggaaacttgg acgtcagtcg ttgaaaaagt agttggaagt
    13441 aaactacaac cagcttatct taccttaacc aatgcttttc caaatcatta cacaagcagt
    13501 ctgctttcag atgaagaatt actgctagcc aaagacccag aatgggtcac aaaagaaatg
    13561 gctgctttag tccaagcagt aaaatggatc cagcaagaac aacatacatc tagattgaga
    13621 acagaattgg agccttggtt acaagcaatc acagaaagtt ggactttctg gcaagaatgg
    13681 caattaaaaa aagatccaaa agaagcagaa gaatggttgg ctaagaaaaa aacacaccgc
    13741 ataggacgtg atttagtaac tgcccattta gagcaaatta tcaaaagtta gatatcttaa
    13801 aaaataaaaa gcaagaaaaa ggagaacaaa atggtcaaga gattaattag tgcaaactac
    13861 agtgatgtca agaaaatgac gtcaaaagag ttgaagcaat ccatcaaagc gagtgaagga
    13921 cgggtgattg tttgtgaaac ggttgctaat actcttccac aatctggaga aattacgaat
    13981 gcagaagttg cgtcagcatt tggtgcagat ttgattcttt taaatggttt cgattgttat
    14041 cgtcctgcag tttttggact accaaatgtg acgattgaga aggtttttga tctacagcaa
    14101 acgattgaaa acccgattcc taaattaaaa gaactagtcg gtcgtcctgt gggaattaat
    14161 ttagaaccca ttgcccctaa aacaaatatg atgagtgaga aagtagaaat cagccaagga
    14221 agagcaagct ctgttgaaac gatacaaaaa gcagaagaac taggagtcga ttttatttgc
    14281 tttaccggta atccaggcac tggagtaacc aatttagaaa ttactaaatc cgtaaaaatg
    14341 gcaaaaaagt atttttcggg attgattatt gctgggaaaa tgcatagcgc cggctcagaa
    14401 gagccagttg tgaataaaga ggcgattatt tcttatgcgg aagcaggcgc agatatttta
    14461 ctagttcctt cagtagggtc tatcccaggc tttttagagc aagacctaat tgaagcggtt
    14521 cggttggcaa aaaaatatga tttgttaacg atgtctgcaa tcggtactag tcaggaaagt
    14581 gcccgaccag aaacattgcg gcaattagcc attatgaata aaagttgtgg tgttgattta
    14641 caacatattg gagatgctgg atacggggga ttggcaccag cagaaaatat tttcgaaacc
    14701 tcggttgcaa ttagaggaat gcgacatacc atcaactgcg ttagccgctc gatcaatcgc
    14761 taacaaaatt aaataaaaaa tggttaaatt gattatttaa gtgggaacat acttgagata
    14821 tcagctaact tctgtcactt taaaaaaggg actgtgacag aagtgaaaat agacaaaaaa
    14881 tatccgaaca agagactagg atagcagaaa aactatcgtt ttgcaacgag gaccaactgg
    14941 accgtaggag caattctctt attgttcgga tatttcgctt gttcaaggac ttatgtctgt
    15001 agtgcccttt tttggtagga aaaatggtag ggaaccatat catttttaat gaaaatcaag
    15061 catttcaaaa gt
//
LOCUS       DAYYFU010000059        14525 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000059 DAYYFU010000000
VERSION     DAYYFU010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.062"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(330..692)
                     /locus_tag="ACHA34_002186"
     CDS             complement(330..692)
                     /locus_tag="ACHA34_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792794.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(766..1146)
                     /locus_tag="ACHA34_002187"
     CDS             complement(766..1146)
                     /locus_tag="ACHA34_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFM8792795.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1228..2403
                     /locus_tag="ACHA34_002188"
     CDS             1228..2403
                     /locus_tag="ACHA34_002188"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFM8792796.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2480..3064)
                     /locus_tag="ACHA34_002189"
     CDS             complement(2480..3064)
                     /locus_tag="ACHA34_002189"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8792797.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3265..4263
                     /locus_tag="ACHA34_002190"
     CDS             3265..4263
                     /locus_tag="ACHA34_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8792798.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4375..4884
                     /locus_tag="ACHA34_002191"
     CDS             4375..4884
                     /locus_tag="ACHA34_002191"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFM8792799.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4914..5573)
                     /locus_tag="ACHA34_002192"
     CDS             complement(4914..5573)
                     /locus_tag="ACHA34_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFM8792800.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5575..6339)
                     /locus_tag="ACHA34_002193"
     CDS             complement(5575..6339)
                     /locus_tag="ACHA34_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFM8792801.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6347..7024)
                     /locus_tag="ACHA34_002194"
     CDS             complement(6347..7024)
                     /locus_tag="ACHA34_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFM8792802.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7061..8446)
                     /locus_tag="ACHA34_002195"
     CDS             complement(7061..8446)
                     /locus_tag="ACHA34_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFM8792803.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8656..8740)
                     /gene="ffs"
                     /locus_tag="ACHA34_002196"
     ncRNA           complement(8656..8740)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHA34_002196"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8763..9278)
                     /gene="tadA"
                     /locus_tag="ACHA34_002197"
     CDS             complement(8763..9278)
                     /gene="tadA"
                     /locus_tag="ACHA34_002197"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFM8792804.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9385..10242
                     /locus_tag="ACHA34_002198"
     CDS             9385..10242
                     /locus_tag="ACHA34_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8792805.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10323..10658)
                     /locus_tag="ACHA34_002199"
     CDS             complement(10323..10658)
                     /locus_tag="ACHA34_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792806.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10725..12095)
                     /locus_tag="ACHA34_002200"
     CDS             complement(10725..12095)
                     /locus_tag="ACHA34_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFM8792807.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12173..12709)
                     /locus_tag="ACHA34_002201"
     CDS             complement(12173..12709)
                     /locus_tag="ACHA34_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8792808.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13018..14499
                     /locus_tag="ACHA34_002202"
     CDS             13018..14499
                     /locus_tag="ACHA34_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8792809.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4475 a         2891 c         2358 g         4801 t
ORIGIN      
        1 actcagttat cttatcatgt cgttagtaac atgtcaataa ctatttttta aattttttag
       61 aaatgtgata taacatccac ttcagaatca acgctgttaa atttatcatg tatttcttcc
      121 ttggtcaact ataaattaag cattactttg tgaaatgatt tttcgatcaa ttattattat
      181 tcgttataca accgttttca acatattttg aaaataaaat gcactaaaaa actatcttta
      241 gcagaacatt tcattgtatt ttttggacca aacaataaaa aaagaagttt gaaaaaaatt
      301 caaacttctt tgttcgctta atctgtattt tagaagaaag gcactccctc tacttcgtaa
      361 gttccaagaa cagagcgcat ttgacctggt tcttcccaaa tatatccttc gcctaattct
      421 tctctttgat tcttttgttc agtttcaaag acaaacaacg gaacattttc ctctttaata
      481 tgtgtatttg tcaattccat caaactaatc ttgctaacat cgatatgaac tatttctttt
      541 aagaaattaa gtatattcgc taaaccagtc tttccttcag aaagttccgc taacgcaaat
      601 tccaacttgt catcaattga atggaccaaa aattttttag taccatcctc caaatgcaac
      661 ataattgtac cagcaactct tttgcttgcc aaaatttcca cctcgatcaa cttgttctat
      721 ggcttacata aaaattctag cacattttca gaaaaaatac actttttatt tcttattttc
      781 tttccgttct agttcagata atgcttcttc tatccaaata ggtaactgct ccgcaatagt
      841 ggtaaatccg atcttacgat ttttattcgt gaaatacctt tttctgcgat atggaacagc
      901 tggatttgtt ttttctaacg ttcttaaaga taaactgatt tttttggaat attcatcaat
      961 gtcgatgatc tgaactgtta ctggctgtcc gacttttaac aatgaatgga tatttttcgt
     1021 ataaccggat tgtacttctg aaacatgaat aagtccttgc gtctcgttat caagagaaac
     1081 gaatgcgcca taaggctgta tccctgtcac ttttccttca attatttgtc caattttata
     1141 agtcatctac ttccctctct tcgcatttat cattatactg taaatagctc aaaaaaagaa
     1201 gcgacgaaaa tgactggagg tcttaagatg atcgaatttg ataaacagat ttcaagaaaa
     1261 gatacagaaa gtgtaaaatg ggacgcaatt gctgagacat atcaaatgga tgacttactt
     1321 cctttgtggg tagcagatat ggacttcttg tctcccggtg gggtagctaa agcgtttagt
     1381 gaatacatca aacatggaat ctttggttat gccactctgt ctgataaact atatcaggct
     1441 atcattcatt gggaacgcca acaccatgct gtggagttga cgaaagaaaa tatcgttttt
     1501 acaagtggtg tgttaaccag tttagctgca gcagtacaaa cttttacaaa accaggcgat
     1561 tctattttga ttcatgaccc agtttatcct ccattttcct ctattataga aacaaatcaa
     1621 cgtcacatcg ttcgaagctc tttgctagaa aaaaataatc atttcatcat ggacttgtct
     1681 gacatggaga aaaaaatcga agaaaacaat gtaaaagcaa tgattttatg caatcctcac
     1741 aatcctggag ggcgtgtttg gagcaaagaa gaattgaaac agttaagcga attgtgttta
     1801 aaacatcaag tgattctgtt tagtgacgag atccatcaag atttaacttt attcgatcat
     1861 acatttactt ccatgctaac gattgatcct aatttagatg atctactagt tgcttttact
     1921 tccgcaacca agactttcaa tctagctgca atcaaaaatt caatggtatt tatcaaaaat
     1981 ccagagatga aagctgattt tgaaaaacat ttagtgatga accaacagca cgaaatcaac
     2041 acttttggac tgattggaac ccaagcggct tatgagacgg gtggagaatg gttaagccaa
     2101 ctgattcctt atctagaaaa aaatgttcaa acagttaatc agttttttga acagtatctt
     2161 ccgaaagtga gaataatgaa ccctgaagga acttacttaa tgtggttaga ttttaccgcc
     2221 tatatttcag acgaccaaga attggagaac actcttgttc ggaaaggaaa agtcgtatta
     2281 aatcctggca tcacgtttgg tccttccggt catgggcata tgcgcttgaa tattgcttgt
     2341 ccagaagaaa ctttactgga aggacttaat cgaatcaagt atgctttaga gacaatcgat
     2401 taaatcattt attcaaaata aaaggacgag accttactag gtttcgtcct ttttcataat
     2461 tactccgtac cataccaaac tattctgaaa tttcaattgt ttcaatcact acatcatgta
     2521 caggtcggtc ttgtggacca cgttgtacat ttgcgatttc atctactgtg tccatccctt
     2581 ctagtacatg tccaaagact gtatgtcgga aatctagcca aggagttccg ccacctttat
     2641 acgcttcaat gatttctgat gggaatccag caccttctag ttgtccaagc atattagctg
     2701 gcacatttgt gttatttaca ataaagaatt ggcttccgtt tgtgtttgga cctgaatttg
     2761 ccatagataa agctccgcga aggttgaaca gttcacgact gaattcatct tcaaaacttt
     2821 cgccataaat actttcgcca cccattcctg ttccagttgg atcgccgcct tggatcatga
     2881 aatcaggaat aacgcggtga aaaatcacgc catcataata accttttttg gaaagttcaa
     2941 tgaaattttg aaccgtcttc ggtgctagtt cagggaataa ttgaacagta atgtcaccac
     3001 ggtttgtttt gataacagct tttggtcctt tttcactttt taaatctagt tgtgggaatg
     3061 ccatattgtt tcctcctaat atttgcatac ttcataataa taataacata atacctaacc
     3121 tctcgtaaaa atactagatt tgttttcttt tatttttcat cactagatac attctataac
     3181 ttaccgtttc gatttcgaat tcaaacattt ttgagaagca ttctcaatta aattctgcta
     3241 tagtgtgaaa agggaggaat ttttatggaa atctatgaca ttacaatcat cggtgcaggt
     3301 cctgtaggga tgtttgccgc tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt
     3361 gacagtctac ctcaacttgg tggtcaatta gcaactttgt atcccgaaaa atatatttac
     3421 gatattcctg ggtatccagc aataaaagca agtgaattaa tcgatcaatt agaaaaacaa
     3481 ttaactactt ttaaccatac attccattta aaagaagaag ttctctcgct tactcgtgaa
     3541 gacgaagtta tcgagattac tacaaataaa ggcatccatt attccaaagc agtcatttta
     3601 gctttaggaa atggttcatt ccagccaaga aaattgaatt tagataatgc tgaatcattt
     3661 gaaaaccacg gattggatta tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc
     3721 gctattgctg gaggtggtga ttctgctatc gattggacgt tgatgttaga gcctattgca
     3781 agcgaagttt acttgattca tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca
     3841 cgcttgaaat cttcatctgt caacttgctt actccctatc tcatcgatgg attatcaggc
     3901 aataatgggg aactgacaga tatccggtta aaaaaagtga aaagtgacga gacgattgat
     3961 ttgatgattg attctctgat cgtcaattat ggattctctt ctaatttaga acatctttct
     4021 tcttggggac ttgatagtac cagaaatgcc attactgtaa aatcagatat gtctacttct
     4081 attccaggcg tttatgcagc tggtgacatc tgtacctatg aaggaaaagt gaaactgatt
     4141 gctactgggc tcggtgaagc acctactgca gtgaacaacg ctttacacta tattaatcca
     4201 aaggaacgaa ctcagcctgg tcacagcaca agcttatacg ataaaaatct tcgtcctagt
     4261 tgatttttta tatctctttg catttcaagt taaacccgaa agcagtttct tgcatttttt
     4321 tcatgttccg ttaacatgaa atagagaaag aaaaaataaa gggggagcaa taatatggtc
     4381 gtcaaaactg agactttaga agaaaaggca agagaactat tgaaggaacg tggcgtaact
     4441 gtagaagata tcgcacaact tgtcatgttt ttacaaaaag actatatttc tgatttgact
     4501 ttggcggatt gtactgaaag tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc
     4561 attatcactg gaatccaatt agatatatta gcagaaaaga aaaaattgat gagtccgttg
     4621 caacatatta tcgaagatga cgaaggcctt tacggtatcg atgagatcct agccttgtca
     4681 atcgtgaatg tttacggtac gatcggcttt acgaattatg gttatatcga caaggtgaaa
     4741 cctggtattt taaaagaact aaacagccac gatggcgttc atgtccatac cttcttagat
     4801 gatatcgtcg gagcgatcgc cgctgctgct gccagtcgat tagcacacaa agaaccagaa
     4861 aaaagaagtc gccttactca ataattcaac aaaaaaagaa agaaaacgtc tatttagacg
     4921 agttctttct tttttgtttg tttcaagcta ttttttccta agaaaagaaa aacagcaaaa
     4981 aataattcta ataatacaaa aaataagata aagctatgca tgaaaaattc ttctggaagt
     5041 acattgatga ttggatcagt aacgggatcg aataaccagt catcgttgtt gaaaaacgtt
     5101 tcatgaaaaa ggataaaaaa gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact
     5161 ggcagtaaca tgccgatttg aaacggacga atcaaccgcc aaagccgatc attcttttgc
     5221 aaatatttga taaacaaaat actcgggatc aaagtgatga aaaacacagc ataatccact
     5281 aaaaacaaaa ttttcacttc ataaaagtga tgggctccgc tagcagaaac tgggaaatct
     5341 ggcaagctga ggatggtttt gaagggatta ttgagataat tcattaggtg atcaaaattt
     5401 ttcagtaacg tctcttgatc taaagaagta taatccaaaa tttgcaaatg tccgatatca
     5461 aatacgtata gtggtcgaaa gttgatcgtc acaccaactg ccagactgat cagtgtcaga
     5521 aataggctga acaatccagc atattccatc cactgccatc tcgaattttt catattagaa
     5581 gctccattca tccagtgatt gtttcacata ggtagctggg ataggcaacc caggtacatc
     5641 ttcttctttt gtaaaacctg ataaaacgag caaagtatct atattattgc gtatcccagc
     5701 ttggatatct gtttcatagt tatctccaac catgattact tcttcttttt gcagccctaa
     5761 tacttcaacg gctttatcca taatgatggc ttctggtttt ccgatgtaga tcggcggcgt
     5821 ttgggttgct gtctctacaa aagagatgac tgaccctgct ccaggaagaa ggccgcgttc
     5881 agtaggtata ttcttatctg ggttcgttcc aatgaaagta gcaccttttt gaatagcaag
     5941 tgttgcttta actacttttt cataggtcag ataattatct aagcctacca ctacatagtc
     6001 aggagcctct tcttcccatg taaatccggc agctaaaatc aagtcaatca atcctgcttc
     6061 tccgataaca tagacttttt tcccttttcc atctgctttc ataaaatcga ttgttgccaa
     6121 agtagctgtg tagactgtct ctggagctac atgaatgtca aactcatctg ctagacgatt
     6181 agctactgtt tctggtgact tagtcgtgtt attggtaaca aacaagaaag gtaattttct
     6241 tttttgcaat tcttccacaa atcgtttacc ggccggtatg ggttgtgtcc cacgataaat
     6301 cgttccgtct aaatcaatta aatatccttt atagtccatt catccactac tcttcccttt
     6361 ttctgatcgt aaatgctgtt tttgtttttt ctacttgttt aggttgttct tgttttttta
     6421 ctttttcaga ggtttttgct gactctgttt ttcgattttt aataaccggt tttttgcgat
     6481 tagatgcgac tggtcctttt ttctcatcga tatacgcacg attatggttt tttttctttt
     6541 ttcggcgctg agttgtcttt tcttttttac cgccgattct ctcgatgaca aaatacgcac
     6601 aaccaaaatt gcaatattcg taaagatagt cttccaagga atcgattcgc tgatctggat
     6661 gcgcccgtcg attatccgct tggaaaaatc cttttaggcg tagttgttca taaccccaat
     6721 ctcctacgat ataatcatat cgggctagta catcactgta acgttctcca agcttttctg
     6781 cattaaatcc ttcacgataa tcggacacaa gccgataatg tctttcacct atcaaaaaat
     6841 tactgtcatc taacagtgtc actagctcgc ctttgacttt ttctgcttta ggctcctcaa
     6901 cgatttcttc caaagcagct gtcagttctt cggtgactgt tgtttcctgt tcagctgatg
     6961 attttttctt agcaatttct gttttatgct caacgttctg ctgttcattt tgttttgcag
     7021 acatgcgttc accacctatc ttttttatta taccttgttt ttatttgatt gggtagtgag
     7081 ctttcaagta atctcctacc acacttcgga taaattcagg gaacaaaaat tcattttttc
     7141 cagcaatctc aatcgttgga aaaaatggaa caaacatcaa atgatctacg gtagtaaaag
     7201 tataattttt attcagatcg attggcttgt tcttccaaaa gacttgatgg ttaatagaat
     7261 catatgtgat tccattatag accatctgcc caaaaatctt accgcgaaat cccattccct
     7321 tcataggata gttacgtaaa aagttacgat ttttttctat ttctaaaact aagcggatca
     7381 gatcatttcc acccaatgtg acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa
     7441 gctgatcctg attgacccgc ccttttggaa tttcagttaa aaacagacca ctgtttaata
     7501 ttgctgcttc cgtctttcct cgttctttca ccgcctcaaa ggctacttgg ataaacgaat
     7561 gctcattgaa gatatctaga ctcagagaaa atggcaaatc tgctacttct ttttctttta
     7621 ataattcatg cccacgttct agataactat ttatttcttg ttcatcttct gtaaaagctg
     7681 tcattgtttc ggtagggatg gctttggctg aatgcttaag gatttttttc tcttcatcga
     7741 tggttaaatg gacttcaccg acatactgcc cgtactttcc agcggctgct atttggacat
     7801 cattgattac gtgaccttct ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa
     7861 ttgctggtat ttcttttgca atttggaaat catcctgaat cccgagatga ctcatcaaaa
     7921 ctaaaacatc cacttgtgaa cgtaaatctt cgactaattc tggcaaaata tcataaggat
     7981 tccgtatgtc ccagccattc ggattataag taagcggaaa tggagcagta aatgcaatta
     8041 gtccgatttt cgtctggtgt ttcgttgtaa tgattttata tggtttcgtc catgccggtg
     8101 gctggagcgt atttttatcg aataagttat ccaggatgat atcaaactca gcatgatcgt
     8161 ataaatgatt caactcttct ttggaatttc ctacgccttc attgtttcct atcgtcgctg
     8221 catcataacc aacttcattc atcagcaaga tattttcctg tccattcgtt gcttctgaca
     8281 atggatgcca acgatccaca aaatcaccta gatctactgc aatcgttgtc gttttttctt
     8341 tcttttcgtt ttctcgttta cgttgatcca aaaaccttct gatccttggc caattttcta
     8401 aatgagaatg cagatcattc gtatgtaaaa tcactatttt ttccatgtac cctctcgcct
     8461 ctctttatct ttatcatacc ataggcagct ttttcttcca ttgaaacaag ctctatttca
     8521 gagtttttcc tgccttgaag caaattgtat aagtacaata ttcgataaat cagtctggct
     8581 aaaaaatcag gaacattcat ttttacagta ctataaaaat aaaaaaaaca gacccgaaag
     8641 tctgttgtcg tttcattata atcagacaca tggcggaacc tgcttagtgc tgcttccttc
     8701 cagacctgac acgcttcaca agcccaccat tgtcctagcc tttcggcaaa gaatagtata
     8761 ccttattttt cattcgttga ccagtctttt tttaatttct tttttcgctg gcggagcttt
     8821 cggaaaaagt cagtcaaaat catcccacat tcgtcttcta aaatgccacc ttctacatat
     8881 gcttggtggt taaaccgctc gtcctccaaa agattcatca aggtaccggc tgttccgcct
     8941 tttggatcat acgcgccgaa ataaacttct gctacacgag acaaaatcat cgcgccgctg
     9001 cacataggac aaggctctaa ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc
     9061 actttttcac aggcttcctt gattgcaagc atttctgcat gtgtagtagc atcttgcgtg
     9121 gtttctctaa ggttatatcc tcttccaatc acttttccat ccaatacaac cactgctcca
     9181 atcggaactt catgaagcat ttctgctttc tttgcttctg ccaatgcaag gcgcatccat
     9241 ttttcttttt ctactgcatc aagatttgta tttaccaatt acccttccct gcctttgttt
     9301 tttcttgttt tactttagca tatgataaaa aggctgtaac taactttttt ttctatggta
     9361 aaataaatac aagaggtgaa caagatgaaa atggataaat tacttgctct ttacccacaa
     9421 gcacagaaaa aaaactctcc agcatcagaa gcggatatcc tttcttttgc aattgatgat
     9481 gcttttatct ggatcaaaca agattcatta tctcaacaag aaaccagcct tttgaaagcg
     9541 ttattccctg taatcaacga tcaaaaaaaa cacccatggt atcgctatct ttttaaagat
     9601 gcttcttgtg ttcaagaaaa aagctttcgt ttgatccagc ttcacgtcga atcaaaagga
     9661 gaattcttaa aaaaagaatg gcaagatact attttggaat tatttcgcta ttctgctgat
     9721 ttcttttttt atacagacac agatgcgctc ttagtcgaac aaataaaccg tgattatctt
     9781 gatgctactg aaatcaacgg tatttttcta tctttagatg ctgattttga tacgacaacg
     9841 tctgtattcg ttggttcttt ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa
     9901 gaaagaaaaa tttttttaac agagcaaaac aatctttata ctcacagccg tactttttct
     9961 atgcaagatg tggctttaca ttactataca aaagatactg taaaagatag taagttgatc
    10021 aaaagttaca tagaactgat caaacaaaat gaaatggaag tgatcattca agaattatgg
    10081 aatgaattag gcaatgtcag ctctgctgca aaaacactgt ttcttcatcg taatactcta
    10141 aaatatcgaa tcgaaaaatt tcaagaacag tctggtttca atctaaaaaa agccaacgat
    10201 ttactgtttt gctatctttt attgcttcat gaacataatt aaagatagat taaaggggtt
    10261 gtgacaataa ttttgtcaca accccttatt tcgaatgaat agtgttcaaa agctagtttc
    10321 tcttacatca ttttttcaac aaaatgcatt agccggtctt ttacatcttc ggttgaatct
    10381 ttgattgaat caccataaac agagattttc ttttttcctg atttgatttt tgctaacatt
    10441 cccatcaaag aatccaagtc actatctgaa agaccatcta cgatttctag taatttttca
    10501 tttccatcaa atttgtcata gacgaatttt tttactttcg tccgagtagt catatgatgg
    10561 atcttttcca ttacttttcc cgatacaacg actgctgtcg ctacactggc agcggctgct
    10621 acgcttaatc cgattgttac ttttgttgat gttttcataa gtatcagctc ctttttatct
    10681 atctccatca tagcatagac aaaaaaataa acgaactaat ccactcagtg aaatctcaat
    10741 cctttaggga aaaagttctt tactgttcct tttacttgtt tcccaaatcc gacaggtatc
    10801 ttatctaata ctagcaaaac ccatccttga ttcccatcac gttgaaatgt ttctcctgct
    10861 gtgtaagatt gccattcctt ttgagtgatc ggcaagcaag gtatattctc tatctttttt
    10921 gtagcaagcg ctaatgcata gctaggttca aatcgattct ttttgaaatc tcctaaatgc
    10981 aaacctgtac gtacgacttt taaaccatcg agagaagggg ctaactcagg aacttcccaa
    11041 agatggtcgt tgaacactaa aagtcttcca gtagcttcat aatgaaaatc atttgaaaac
    11101 tccgtccaga gtttttcctg ctcttttgtc atttgcactt tacttttttt tcgtgtcttc
    11161 ttttctttat gcatctgatt ttgtccatga aaagtcagct ttgcaacaaa atgtccttca
    11221 ccctgatctt tatgaggcca tatacgtatt gttttttcca aaccagctac gcttccccat
    11281 tcagaacgtc ctgaactaac ggattgagtc aaaggaatct cctcaatcgt tactggataa
    11341 ttttctacaa gccagctaat gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa
    11401 taaatcagtt gtcctttatt ttttagcatt tttattgctg aagacaaaat ctcttgctga
    11461 cgtttttgac agtatagcgg agattcttct gtccactctt ttattgcatt tggatctttt
    11521 cggaacattc cttctcctga acaaggagca tcaaccacga tccgatcaaa aaagcccgaa
    11581 aaatgaggga caagttctgc gggtgcatga tttgtcacta ttgcgttcga aactccccat
    11641 cgttctatat tttctgataa aatcttcgca cgtttcggaa agatctcatt cgtcactaat
    11701 aaccctttcc ccttcatttg agcagctaat tgggttgatt taccaccggg agccgcacac
    11761 aaatccaaaa ctttttcacc gggttttgct gcagcagctg ttccaacgat cattgcactt
    11821 ggttcttggc tatattcata cccagcttga tggaggaaag actttccatt gacagtgcca
    11881 agaaatccct cattgctgta gggagcgggt tgtaattctt cgctatggta agtttggacc
    11941 atgtccaatc ctgccggttt tagtggattc caccgaaatc cttttttcac gcttccttgt
    12001 tcaagcgcag aaaaaaaatc ggatgcttct tcgcctaaaa gaagacgata ttttttgata
    12061 aattgctgtg gtaacgttgt cgcttcctcc ttcattccaa tgtttcgtta gacattttac
    12121 aatctttttt acgaaaaata aaggtggctg atctactttt tcatgttgct gcctaaggtg
    12181 actgttcctt taagatattt tccaagtatt catccgtcac ctcgttttta tcccaaacag
    12241 cgttcatcat ttcaagaaaa tcttgagaag aatcatctga gcgcaaactt cccaatgcat
    12301 taagccatac caattcaagc gacttttctt ctttgatctt cgtgtgacct ttgacatatg
    12361 agaaaaatgg ccgcttaaat agcgaatcca tgtctaaatc agattttgca atgatttcac
    12421 gaatactttc atggatagaa aaaaaatctt tcaataagta gacaatgctt aaaacatcat
    12481 cggaactgca ttcagctaag tagttttcaa aataaacagt gatttcttct aatatcttct
    12541 cttttaaccc gctgattgat ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga
    12601 actctgctaa ctctcttgca gtaattgact tcattccata ttcttccacg tataccatac
    12661 ttttgaacag caaatattct ttactataat ttgttactct ccccatcatt cttacctccg
    12721 tctcttttca tctacccata aataaataac ataaataatt ctaaaaatat tattgataat
    12781 tatcaaaaca tatttagtta tatccattaa aaatcaattt ttccgcctta aaaacaagaa
    12841 tatcttgaca aaatacccta ggaaataaac aatagaataa acaaggtaac aaaaaaatta
    12901 aataacgtta tataaaaaag aaggcgacac tctgtgagaa taacacagac tgaaatgact
    12961 ttccactcac agtttttgtc taactatata caaaaacaga ataaaaagga ggtatttatg
    13021 ctaattgaag caagaaaaaa agagttactt gttctacaaa tggtcattca aaaaaataat
    13081 tgttctttac aagaattatc caatgactta catattccaa aacggacaat caaagaactg
    13141 atccgaaaaa taaatgtatc actcaagcaa tttttattga ttgatgaatt tatttattct
    13201 aatcataaag gagagatcca aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt
    13261 tctaagttaa aattatgcta cctaaaagaa tccaatcgtt ttaactattt gatgttacta
    13321 atcaattatc cacaaactgc tgtaccaaaa aaatatttac ttgaacaact ttatatatcg
    13381 ccctcctatc tcgaaaaact gacaaaacag ctcaatcatt ccttaaaaaa attccattta
    13441 cggatcctat ctgtaaaaaa ttgctgtact cttgaaggaa atgagttatc tatccgtctc
    13501 tatctttact tttttctttc agatacattt ggaggtaacg attggccatt ttcctcactt
    13561 gaattaaatg cactaaaaat gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa
    13621 gaaaaaaata tctacttgct gttaacttta ttcatattac gaaacaaaca acaggcatat
    13681 tttccaaaaa cttcttctga aatcactgaa ttgatggact tattgcagca tacacatgat
    13741 tattcagaat actttacttc tttttcagtg agtcgccctt gtgaagagac agaaacagag
    13801 agagcctact ttaattattt tttctattgt ttttttccaa atcaaatctc ttttcgacaa
    13861 aaacaggaga ttggtttttt attttccaca agcaaaaacc ttatttgtcg acaagtcggt
    13921 caattgatga cttacttaac tcaacagttt cctctcaacc atttagaaaa taaaatacca
    13981 atttattttt attttcttgt gttggcagtt gtttttttcg aactgttggg tgaatccagc
    14041 agtagttttt ctacactttg ctttccagaa gccacgtcca atcattctag ttcttttata
    14101 caggaaaaga aattaagaga aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac
    14161 caaatcagtc aataccttag tcaattgatc caacacttac tacaaacaga aacgaaagag
    14221 cagttactca tccatttgca gataaccaaa cagtttacag gaaattatca tcttcaaagc
    14281 catttgcagc agttatttcg caaagatgct gttaaaatta ccgcatattc agaacatgct
    14341 gatttgatca ttacagatca gcatgagaaa atttcagatg aaacggagac tttctatctg
    14401 gatacaacgc acaatacagt gttatttggc aagttaataa aagcggttca tgaactgtat
    14461 atgaaaaaag gttatgagaa caaaacaaag aggatgtgac aaaattcttg tcacatcctc
    14521 ttatt
//
LOCUS       DAYYFU010000060        14170 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000060 DAYYFU010000000
VERSION     DAYYFU010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.063"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..2323
                     /gene="acm"
                     /locus_tag="ACHA34_002203"
     CDS             158..2323
                     /gene="acm"
                     /locus_tag="ACHA34_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFM8792810.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2595..3080)
                     /locus_tag="ACHA34_002204"
     CDS             complement(2595..3080)
                     /locus_tag="ACHA34_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFM8792811.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3170..3515
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3560..4201
                     /locus_tag="ACHA34_002205"
     CDS             3560..4201
                     /locus_tag="ACHA34_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8792812.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4220..4855
                     /locus_tag="ACHA34_002206"
     CDS             4220..4855
                     /locus_tag="ACHA34_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8792813.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4873..5688
                     /locus_tag="ACHA34_002207"
     CDS             4873..5688
                     /locus_tag="ACHA34_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8792814.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6007..7443
                     /locus_tag="ACHA34_002208"
     CDS             6007..7443
                     /locus_tag="ACHA34_002208"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8792815.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7632..10079
                     /locus_tag="ACHA34_002209"
     CDS             7632..10079
                     /locus_tag="ACHA34_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM8792816.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10309..11376
                     /gene="rlmN"
                     /locus_tag="ACHA34_002210"
     CDS             10309..11376
                     /gene="rlmN"
                     /locus_tag="ACHA34_002210"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM8792817.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11366..11572
                     /locus_tag="ACHA34_002211"
     CDS             11366..11572
                     /locus_tag="ACHA34_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792818.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11569..12672)
                     /locus_tag="ACHA34_002212"
     CDS             complement(11569..12672)
                     /locus_tag="ACHA34_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8792819.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13225..13773
                     /gene="aac(6')"
                     /locus_tag="ACHA34_002213"
     CDS             13225..13773
                     /gene="aac(6')"
                     /locus_tag="ACHA34_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8792820.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            complement(14021..14140)
                     /locus_tag="ACHA34_002214"
     CDS             complement(14021..14140)
                     /locus_tag="ACHA34_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM8792821.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
BASE COUNT         4843 a         2337 c         2992 g         3998 t
ORIGIN      
        1 gttttttggc atatttattt tttataaata tttgtaaaat aactatcaat tttttagtta
       61 aaagattgga aatgagtggg ggaggaattg aagaaaggat ttttgagaga tgatatagta
      121 ggttttattt ttagttaaaa taggggagat agaaaaaatg aaaaagtgta gaaatattat
      181 tttatcaatg ttatttatta ttactaatat aacatcactg attcctgttc atgtctatgc
      241 ggatgcaggc agagatatca gcagtaatgt cacttcgttg accgtggacc caacgaatat
      301 tacagatgga ggaaacataa aagtaaaatt ctcgtttgac gaaaaaaaac aaaatatcca
      361 accaggtgat tatctttgga tcaattggcc aagtgaagga aatattcgcg gtgaaggatt
      421 ccaaaaagaa atcccattaa tgattgaaaa taaaaatgtt ggaactttaa cggtcagaaa
      481 agattctgcg caggttgtgt ttaatgaaaa tattaaaaac ctagactcag tagaaggttg
      541 ggggcaattt gagatacagg ccagaaacgt aaccgatacc ggtgaagaaa atacgggacc
      601 attcaccgtg acgagcggtg ataaaacagc tactgtaaat gtgacaaagc cagcttctgg
      661 ttcttcttct agcgtttttt attataaaac aggagacatg ctacctgaag atactaagca
      721 cattcgatgg tttttgaata ttaacaataa cggcacttat gttgaacaac cagttaaaat
      781 cagtgatgaa atccaaagtg gacaaagatt agatccaagt acgtttgaaa ttaatcaaat
      841 acacttaggt gaacagaagg tttatagagg agaagaggga atccaacaat ttttacaaga
      901 tttccccagc gctacattta atttttcagt aactgataat tacattgaaa taactatacc
      961 aaaaaacttt gtcaatctta ggaaaataat ggtgagttat aagacgatca tagaaaaccc
     1021 agaacaaata aattttgaga atcattcgga agcttggttt aaagaattta ataaacctgc
     1081 tgttgatgga gaaagtttca atcatacagt taaaaatatt tcagcttctg gtggagtcaa
     1141 cggtacagtt agaggcgaat tgaagatttt caaatatatc aatgacacag agattggtat
     1201 cccaaatgtg actttcgaat taagacgagc agatgaacag cctattcaag gacagtctag
     1261 tatcctgttg acttcgaatg aacagggaga agctagtatc aaaggcctgc aagtaggaga
     1321 ctatgttgta aaagaaaaag aagcacccaa ttggattgat tttgatccac taagttcaaa
     1381 tgaactcaaa ttctccataa acgaaaatga tacagaggga gtcagtttac ctatttataa
     1441 caagaaaaag gttacgaaca tcacagcaac taaaatttgg aacggtggaa ccactccaag
     1501 accgagtatt tattttaaat tattcagagc aagtacaaac aattgggaac cagttcctga
     1561 tgcagaaaca aaacgattag acaatggaat aacttcagtg acttgggaag atatccaaca
     1621 gtatgatgat agtggaaacg aatatacgtt taaagtgcaa gaagtagatc aaaacggaaa
     1681 tgattatgtc ccttcaggtt atcaaaaaat tgaaaacggg ctagtcgtta caaatgagaa
     1741 taaagaagtc atttcagtct caggacaaaa aacatgggaa gacaatgaga accaagacgg
     1801 caaacgaccg acgacgatca cagtaaatct attagccgac ggtcaaccga tccaacacaa
     1861 agaagtaagt gaaaaagatg attggcgcta tcgtttcaca aatctgccaa aatacagaga
     1921 cgggcaagaa atcatctata ctgtaacgga agacaacgtg cccgaataca gtacgacgat
     1981 tgacgggtat aacatcaaaa acagttatac accggaaaaa acaaatatat ctataccagg
     2041 tagccatata acaccgtgga aaaattttcc agagaagaca gagagagaga aaattagaaa
     2101 tctattctct caaaaattgc aaatatttgc tcatacagga gaatccagcg aacaaagaag
     2161 caattatatt agtaagagag ctgtacctaa acagataaca aataaatcaa aaagcattct
     2221 tcctaaaaca agtagtaaaa aatctagttt tgctgtaatc attggtttat tgatagtaac
     2281 tatctgtgct gggatattct tacaaaaata taaaaaagtt tagttagaat ataatatttt
     2341 ttgttaaata tgactgaaat caaaagaata aaattccgat ttataatcga aaaaagaagc
     2401 atttttatat atgcttcttt tttcgtagaa tcaataaata tggatttggc taactatgga
     2461 agaatactcg tattagtttt cagcgttcat actagttgtt acattgtttg attggccaaa
     2521 agaatttctg atatattttg tgctaaataa agaaaaagac gcttgaattg ggaaataaga
     2581 aaactactct atcattacaa attttcataa actggcagat gttctaagta aacaagtatc
     2641 ttttcaaagt cggaaaaatc cataatcaac gtagtggtat tgatatttgg atgaaagcct
     2701 acggtatctt gacgatcaat agcagcatcg atcacgactt ggattttttt ctctttgtcg
     2761 tgcatcaatg ctaatggagt cactgtacct gctggaacgc ctaggaggtc aggaagctgt
     2821 tcttcagaaa caaaagataa gcgcttttct tttaattgtt cagctagttg ttttaaatcg
     2881 gcgtgctttt catcaggcaa gatgacaaga tagatttgtt ttccttttct tgatttcaat
     2941 aatagatttt ttacttgcgg accaggtagg gaaaaaggga catttttgac tgacgtaatc
     3001 gcaggatgat cgactttttg aaaagagata cccagttttt ttagtagttc ataagcttct
     3061 tgttcggttg cacgactcat cgcgcatcac cctccttttt ttattagtgt atcataaaaa
     3121 agagttgaca aatgaaaaat aatcatgtaa attacacgta aagttaaaac aaatgacatc
     3181 gaaaagaaga gtagccgtta ggtgttttta gagagtctct ggttggtgga aagaggcagc
     3241 agttaaccgt gaaacacatc ttggagtcga ttttctgaag cgtatagtag gaaaatccgg
     3301 ggagcccgtt acagctgcgg tcgttgctag atgaccagat aaggctggct tttccgtgag
     3361 gagtcggtaa aaaaaggtgg aaccacgtga ttaacgtcct ttgatcttcg gatcaaagga
     3421 ctttttgttt tcatctgaac ggcaacgact gtgtgaggtt ttttctgcaa ggaaaaaata
     3481 aacagaggtg gaaccacgta taatacgtcc tcttggcatt tgccaagagg atttttttat
     3541 tttaaaggag gaaaagaaga tggagtattt gattgaagtt atgccgcaat tattagtagg
     3601 agcactcaca acattgaaat tatttttatt taccttgatt ggctcattac cattagggat
     3661 tgttttagca ttaggattgt ctttgcattt taagccgcta cgctacttgc ttcaaacata
     3721 tgtatgggtt atgcggggaa caccgctgtt gttgcaactg atttttattt tttatgggtt
     3781 gccgacgatt gggatcgttt ttgatcgttt cgaagcagca ctcattgcct ttatcttaaa
     3841 ctatgcagct tattttgcag agatctttcg tggagggata caatcgattc ctcaaggtca
     3901 atatgaagca gcgcaagttt tgcaattaag tccctttcaa acggtgacga aaatcgtcat
     3961 cccacaagtg acaaagattg ttttaccttc tgtagggaat gaagttatca acttggtcaa
     4021 agattcttcc ttgatctata tattaggttt aggtgacgta atgcgtgctg ggaaaatcgc
     4081 gatggaacgc gacgcgactt tactgccttt gctaggcgtg gcaatcatct atcttacatt
     4141 gactggtatt ctaacaatca taatgaaaca agttgaagga aaattaaatt attaccgcta
     4201 actgatagga ggaagaaata tgattgaatt gaagaaagtg aacaaagcat ttggtgaaaa
     4261 aaaaataatc aaagatttga atctaatcat tccagatggg aaaacattag ctatcgtagg
     4321 tccttccggt ggcggtaaaa ccacgctttt acgtatcttg gctggcttag aaagttctga
     4381 cagcggagaa ttttatttag acggtgtacc gttcaaccca ttcgagctta aaatgaaaga
     4441 acaagttgta ggagtcgtat tccaagactt ccagctattt ccacatctga atatttttga
     4501 aaacattacc ttagcaccaa aaatggtatt gaaacagaca aaagaagaat acaccaaaaa
     4561 agcagaagaa ttagcggctt cattagggat tagtgattta ttgaatatct atccttatca
     4621 gctttctggc ggacaaaaac agcgggtagc gattgccaga gcattggcta tgaatccacg
     4681 ggtattggct tatgatgaac caacaagtgc tttagatcct gaattacgcc agcaagtcga
     4741 gcgtttgatc ctatcattga aagaagatgg ggtaacccaa attattgtca cccatgattt
     4801 agtatttgca gaaaatgtcg gtgatcaatt actaaaagtt tccccaattc attaagaaag
     4861 gagaagatag agatgaaaaa aaccagtttt ctattcgctt tgatcgcagg tcttcttctt
     4921 tttgcaggct gttcgaacaa aaaaacttca gcagataatt gggcgactat caacgaagag
     4981 aaaaaaatcg ttataggttt agacgataca tttgttccta tgggatttag agataaagac
     5041 gggaagctca caggatttga tattgattta gccaaagcag tctttgaaca atatgggatt
     5101 acggtagact ttcagcccat tgactggtca atgaaggaat ttgaactgaa caatggaacg
     5161 atcgatttga tatggaacgg ctattccaaa acgccggcaa gagaaaagaa agtgcaattt
     5221 acaaaacctt atatggaaaa cgatcaagtg ctgatcacac ctaaagaatc aggaatcaca
     5281 aactttggac aaatgaataa taagagatta ggtgcgcaaa atgggtcatc tggttatgat
     5341 gtatttacga aacaaccaga agtattaaaa gagatagtca aaaaccaaga tgctgttttg
     5401 tatgatagtt ttaatgaagc attgatcgat ctgaaaagca gacgggtcga tggtctgctg
     5461 atggataaag tatacgcaga ctattatttg aaacaaagaa acgaactaga agacttcaac
     5521 ataacaaaag gttcttatga gagtgaggat tttgcagttg gtgcacgcaa gtcagatagt
     5581 acattagtga aaaaaatcaa tgaagcccta gatcaactag agaaaacagg tgaattccaa
     5641 aaaatatccg ataaatggtt tggcgaagat gttacgccga gaaattaaca aataggggac
     5701 tgtgacaaaa gacgtgtcac agtccctttt tccgaataaa cagtgttctc ctgccaactc
     5761 cttgtgactc ggagctgaat ggcaggctca caacctcttg gtaaacggtg ttcaaaagcc
     5821 agtttttcgg tattaagcca aatttatgca aaaatgtaga aagccatttt cacaaaattt
     5881 ttcttactac tcgaaactaa gcgacttttt cacaacctcc tttacataat tttattttgt
     5941 tgtggaaggg gtttcgtgtt attctaaaag aggaacgatt gaaggttata ggaaaggaag
     6001 agatgaatgg aacacaaaga acttacctca tttccaaaag attttctgtg gggctctgct
     6061 tctgcagcgt atcaagtaga aggagcttgg caagaagatg ggaaaggaga atcagtctgg
     6121 gatcgatttg tacggatacc tggaaagaca tttaaaggaa cgaacggtga tttggcagta
     6181 gaccactatc atcgttataa agaagatatt gctttaatga aagaacaagg tctgaaagct
     6241 taccgcttct ctgtagcttg gacacggatt tttcctaatg gacgaggaga agtcaatcaa
     6301 gctgggctcg ctttttatga gagattgatc gacgaattga ttgaaaacga tattgaacct
     6361 atgttgactt tatatcattg ggatcttcca caagcattgc aagatgaata taatggttgg
     6421 gaatcacgtc agatcattga agatttcact cattatgcag aaacattatt tgaggcattc
     6481 cgcgggaaag tccattactg gattagtttg aatgaacaaa atatcttcac ttcattaggt
     6541 tatttgttag cagcacatcc gccaggtgtg acagacccga aacgaatgta tgaagttaac
     6601 catattgcta atttagcaaa tgcatctgtc atcaataaat ttcacgagtt agaaatgcct
     6661 ggaaaaatcg gaccaagctt tgcgtatacg ccaaattatc caattgacag taatcctgaa
     6721 aatgttctgg ctgctgaaaa tgcagaagac ctgatggcca attattggat ggatgtctat
     6781 atgtggggaa aatatccgat tgctgccatg aggttcttag aagaaaaagg atgggcacca
     6841 acgattgaag ctggcgatgc agaattgcta gaatctgcta aaccagattt tcttgggatc
     6901 aattactatc aaacggcgac gaatgcattc aatccattaa acggagttgg tgctggaaaa
     6961 atgaatacga caggaaaaaa aggttcatca gaagaaactg gagttcctgg aatgtataaa
     7021 aaagttgaga atccatttgt cgaacgaaca aattgggact gggagattga cccagaagga
     7081 ttaaggatcg gactgcgtcg tatcacaagc cgttaccgca tgccggtact gattacggaa
     7141 aatggcttag gagagtatga caagttgaca gaagataaac aaatccatga cgactatcga
     7201 atcaattact tacaaagcca cgtcaaagcc atcaaagaag caatttcgga tggagcagaa
     7261 gtcctaggct attgcacctg gtcttataca gatttgctta gctggctgaa cggttatcaa
     7321 aagcgatatg gatttgtata tgttgatcaa gacgaaacac aagaaggatc tttggaacga
     7381 tataagaaag acagttatta ctggtaccaa aaactgattg aagaaaatgg agagaacctt
     7441 tagttcacat acttaaaaca agaaggtacg gctggatatg gccgtgcctt ttcttgataa
     7501 aatcatttag aaattaagaa aaactgtaaa ataagctgaa aaatgaaaga aagcgtcagt
     7561 tctttcaaac aaaactttag ttcccttact tttttttgta taatgaaact tgatgaaatg
     7621 agggataata aatggcacaa aagaaacggc ctactaaaaa aaggaaaaag acgaaaaaaa
     7681 cgaaaaagca aaaacagcag caggaacaaa tgctgacgat tggtgttggg tttgccttga
     7741 ttttatttgc tgtcttcggc tttttaaaat taggtttttt aggaatattg attgcaaacg
     7801 gctttcggat cattgcagga aatacttacc aaatcctctg tcttttatta gccgtattag
     7861 gattttggat tgtcattaag aatacagagt tttcaattgg taaaaaccga cgctggtttg
     7921 gcggcatact cttttatttc ggtatcttat tattgttaca tgcccattta tttggcaaac
     7981 tccatactgg ggaaccaaat atcatgggga cgacatggga tttgttagcc agtgatataa
     8041 aacaaagtca agtagacaat aacgttggtg gtgggatgat tggagcaatc ctttatcatt
     8101 ttacttactt cttgattgca cagcctggaa gttatttagc agcagtgcta ttactggcag
     8161 gaggagcatt cctgttcagt aatctggaag gctatcagct gttaaatggt atccaatcaa
     8221 ttggagaacg agtacaggaa ttattggaag gtgatcctgc caagcaagct cgaaagcaag
     8281 cggcaaagga agagagaatg aaacagcgcg cagaagcgaa agaagcaagg cgcttagctg
     8341 caaaagaagc tgcagaaaaa gaagctgcag aatatgagaa gaaaaaagcg gtgagccagc
     8401 aaagggaaaa tactgctgat gaatggcagg aaccagaaaa tacagagccg gagcagctaa
     8461 gttttgttcc aatcgacagt tttcaagaaa atatccatcc tgcgaatcta gagaaaccag
     8521 taccagatac accaaaacag accaatacag cagaaggttt ttcagatgag ttgccagagg
     8581 atgacggtac ctcattagaa tttgagattg aagcagaaca ggaaaatcaa gactatgaat
     8641 taccgacagt tgatttgctt gattctatac caactgtgga tcaaagtgat gagtataaaa
     8701 aaattgaaaa aaatatcggc gtattagaac aaactttcca aagtttcggt gtcgatgcca
     8761 aggtcgtcaa agccagctta ggaccatccg tcacaaaatt cgaagtccag ccggctgtag
     8821 gggtaaaagt aagtaaaatt gtgaacttaa cagatgatat cgcgcttgct ttagcggcga
     8881 aagatgtccg gatggaagca ccgattccag gtaaatcact gattggtatt gaagtaccaa
     8941 atggaaagat cagtatggtt tcctttagag aaatcatcga agcacaacca aaccacccag
     9001 ataaattact agaagtacct ttaggacgtg atgtttctgg acgggtacaa acggctgatc
     9061 tttcgaagat gcctcactta ctcgtggctg gttcgactgg tagtgggaag tcagtagcaa
     9121 ttaatgggat tatcacaagt atattaatgc gtgctaagcc ccatgaagta aaactgatga
     9181 tgatcgatcc gaaaatggtt gaattgaata tgtacaacgg catacctcat ctgttgacgc
     9241 cggtcgtgac gaaccctaga aaagcagcgc aagccttaca aaaagtagta caagaaatgg
     9301 aagaacgata tgaaaaattc gcggcaaccg gagtaagaaa tatttctggt tacaatgaat
     9361 ttgttcaaca gaaaaacttg gaaaacggaa cgaaacatcc aaccttacca ttcatcgtag
     9421 tgatcgtaga tgaattagct gatttgatga tggtagccag caatgaagtt gaagatgcga
     9481 ttattcgttt agcgcaaatg gctcgggcag cggggattca tatgatccta gctactcaac
     9541 gtccaagtgt ggatgttatc acaggaatca tcaaagcaaa tgtaccttcc agaatggcat
     9601 ttgcggtatc aagcggtaca gattctcgga cgatcattga ttctaatggg gcagaaaagc
     9661 tcttagggcg aggagatatg ctattcttgc caatgggtga gaataaaccg atccgtgtgc
     9721 aaggagcatt catttctgac cacgaagtag aacgggtcgt tcaatttgtt acagatcagc
     9781 aagaagctca ttatgaagag aaaatgatgc cgacagatga agttgaaacg acaggtgctc
     9841 cagaacagcc acaagatgag ctgtttgaag aagcaaaagc acttgtcgtt gaaatgcaaa
     9901 cggcaagtat ttcccttctc caaagaagat ttcggatcgg ttacaaccga gcagcacgtt
     9961 tagtagatga actggaagct catggagttg ttggcccgtc agaaggaagc aagcctcgga
    10021 aagtattcat tgagcaggaa gaaacatcag aaacgttgcc ggacgataat gacatgtaaa
    10081 acttgtgtga tgaagggttg aaaaacgaat aaccaataaa aagacaaggc atgatgatac
    10141 caaaagaaaa aatggttttt ttccatcttt tctaatacgg tatgatgtct tgtcttttta
    10201 gtttttgtga gattaaagtt ctattcgatt tatttgtgaa gtattgcatt aaggaaatga
    10261 atcatgtata gtatgtagcg aaactttgag aaaaagaggg aaaatacttt ggaaaaacag
    10321 tccatttatg gcttgacgaa tgaagaatta atcaattggt ttatagaaaa cggcgagaaa
    10381 aaattccgag ctgcccaagt ttgggaatgg ttgtatcaaa aacgtgtaag taatttcaca
    10441 gaaatgacga atctatcgaa acaattgatc gaaaaattat cggagcattt tatcatcaat
    10501 cctctaaaac agatggtcgt ccaagaagct tcggatggta cagtaaaata tttgtttgaa
    10561 ctccctgata aaaacatgat tgagactgta ctgatgagac aagaatatgg cttgtcagtt
    10621 tgtgtgacga cacaagtagg atgtaatatc ggctgtacgt tttgtgcaag cgggctgctc
    10681 aaaaagaacc gtgatctgac tgctggagaa attgttgcac agatcatgat ggtccaacat
    10741 tattttgatg agcgcaatct tggcgaacgc gtttcacatg tcgttgtaat ggggatcggt
    10801 gagccatttg ataattacga caatgtaatg gactttttgc atatcatcaa tgatgccaaa
    10861 ggattggcaa ttggtgctcg gcacattaca gtttccacta gtggattagc acacaaaatc
    10921 aaggaattcg ctaataacgg cttgcaagtc aatctggcta tttccttgca cgcgccgaat
    10981 aatgaggtgc gaacatcgat catgcggatc aaccgaagtt tcccaattga gaaattgatg
    11041 gaagctgtgg atgagtattt agagaaaaca aaccgtcgaa tcacgtttga atatatcatg
    11101 ttgaatcaag taaatgatag accagagcat gcgcaacaat tagcagatct attaaaagat
    11161 aagaaaaaat tagcatatgt caacttgatt ccatataatc cagtcagtga acatgatcaa
    11221 tattctcgca gtccaaaagc tgatgtattg agattttatg atgtattgaa gaaaaacggc
    11281 gtaaactgtg tgatacgtaa agaacatggt acagatattg atgcagcttg cggacaattg
    11341 cgaagcaagc aaatgaaaaa aacagttgcc aaataatcgt atcctactcg ttttttcaat
    11401 taccataaaa ggagcagttt tcttaattaa ggaactgttc aacttaaata agagaaaaga
    11461 ttctatagcg aaagctataa agcttttttt gacaagaaaa accctcatca agcaaagaaa
    11521 tgtcctcaat ttgatgacag cctgcttgat gggggtttta gcaattattt aactgatttt
    11581 cttatatcct tgctggcttt cttgtgtagc atgataagtt tctacgtcaa gaatggcctg
    11641 tttaaatttt tctcgggtga tttcataagg cacatgttta atatcatttg ttgccatggc
    11701 tttatctaag attgcttcta aatcggtcgt gacattgaga ttcatgtcgg ttaacgatac
    11761 tggaagttgg ttctttttca taaacacata gattttatca aacaaatcga actgctgatc
    11821 catcatcaat aaggtcagta caccataact gacagatgct ccgtgaaggt atccctccat
    11881 tttttctaga acagtcgtac cgtaatagaa tgagtgtgcc atagcagaat tatagttatt
    11941 gttaactaaa acagaaacca atccggtagt aacaatgata tccgaaacga cttgttcaat
    12001 tgcatatgag ggacgatcgt cggcactatc tttcaatgct tgcgtaccat aagtcaagag
    12061 acgatcaatg cacatttttc cgatttggat tcctaatcta tccgtgtagc ctagatcatc
    12121 gccacgagca gaaaaagtag attcgacttc tttggataaa gcatcgccaa tacctgccca
    12181 taagtattta tcaggtgctt cggcaataat tgctgtatca atgaaggtgt gtacaggtgg
    12241 ttttgaagga aagaagagtc cgtccattac gccttcttcg ttatagatga cacataccgc
    12301 agtgacgggc gaacagttcg aaccaatagt gggaaaggtg aagactggtt tagctagttt
    12361 ttcaccaatg acttttacag tatcgcaagc acggccacca ccgactgcaa acaggacgtc
    12421 tgctttttgg acactttcat gctggatcaa ctgttcaata tttccataag ttgaattacc
    12481 cccgtaccag agactggcga caacctctat ttcctgtgtc aaagctactt ttattttgtt
    12541 ttctgccttt gaaagtgcag tcttccctcc aatcagcact acttttgtcc catattcttt
    12601 gacgattcca tttgcttttt catacgcatc tgttccaatg gtataatttg gtaagaaaac
    12661 actttgactc atattcattt ccttctttct tcgttgaaac gggttaaaat tttaaagagt
    12721 gatcgatctg actgcggctg tagatggagt agaggtgaac ctaagacagg ataagtgatg
    12781 aataaggtgg catgaacacc ttgaaatcat caataagtat cctaaaaacg aacaagtaca
    12841 aaaacattga aaacgacaat aatgaatttt tgttcctctt agtatttcta ccgattccct
    12901 ttgctttttt gtaattatct aaaaattcta aaaattaaga ttattatatg aaaacaggtt
    12961 ttatttgtca attcataatt ggtttttttt aaagcaaaag ataatctata tcatgatgaa
    13021 caaacagagt tttttcctcc aattgatggc tttcctgatc tttcctattt tgaataaagt
    13081 gtttctgtat aattttttta ttttataagt aagataaata atgaaagaat agagatactg
    13141 attggtagct aaagattttt gagcgagaac atttcgtttt ttcaaaagga ctgttagaat
    13201 aagcaagaac aagggggaag acgtatgata atcagtgaat ttgaccgtaa taatccagta
    13261 ttgaaagatc agctttctga tttactgaga ctgacttggc cggaagaata tggagacagc
    13321 tcggcagaag aagtagaaga aatgatgaat ccagaacgaa tcgcggtagc agcggtagac
    13381 caagatgagt tagtaggatt tattggtgca atccctcaat acggtatcac aggttgggaa
    13441 ttgcatccat tagttgtaga aagctcccga cgaaagaacc aaataggtac tcgattagtc
    13501 aattacttag aaaaagaagt agcttccaga ggaggaatca cgatttattt aggtacggat
    13561 gatttagacc atggaacaac gttaagtcaa acggacctgt atgagcatac atttgataaa
    13621 gtggcttcca tccagaacct tcgtgaacat ccgtatgaat tctatgaaaa attaggttat
    13681 aaaatcgtag gtgtcttacc aaatgcaaat ggctgggaca aaccggatat ttggatggca
    13741 aaaacgatta ttcctcgacc aaattctcaa taataaaaag aggttgtgac agaagtaatc
    13801 agcttcaaga aataagaagg aatttacgaa aattacttct tgaatttttg tgaaattcca
    13861 acttatttcc gaagaagcta cttttggaac accgtgtacc agaggttgtg aaaaagtcgc
    13921 ttagtttcga gtagtaagaa aaattttgtg aaaatggctt tctacatttt tgcataaatt
    13981 tggcttaata ccgagaaact ggcttttgaa caccgtttat tcagataagg gactgtgaca
    14041 agacttttgt cacagtttct ttttgtttat tcttttttat ctttgtatgg caataataga
    14101 caatataacg aaaattattt aatggctttt ggaagaccat ggaaaacctt gtacaaagct
    14161 attttttctg
//
LOCUS       DAYYFU010000061        13949 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000061 DAYYFU010000000
VERSION     DAYYFU010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.064"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            148..486
                     /locus_tag="ACHA34_002215"
     CDS             148..486
                     /locus_tag="ACHA34_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792822.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(691..1266)
                     /locus_tag="ACHA34_002216"
     CDS             complement(691..1266)
                     /locus_tag="ACHA34_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HFM8792823.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1998..2579)
                     /locus_tag="ACHA34_002217"
     CDS             complement(1998..2579)
                     /locus_tag="ACHA34_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8792824.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2762..3388)
                     /locus_tag="ACHA34_002218"
     CDS             complement(2762..3388)
                     /locus_tag="ACHA34_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8792825.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3410..4741)
                     /locus_tag="ACHA34_002219"
     CDS             complement(3410..4741)
                     /locus_tag="ACHA34_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFM8792826.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4896..5297)
                     /locus_tag="ACHA34_002220"
     CDS             complement(4896..5297)
                     /locus_tag="ACHA34_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8792827.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5383..5589)
                     /locus_tag="ACHA34_002221"
     CDS             complement(5383..5589)
                     /locus_tag="ACHA34_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792828.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5684..7186)
                     /locus_tag="ACHA34_002222"
     CDS             complement(5684..7186)
                     /locus_tag="ACHA34_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFM8792829.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7204..7659)
                     /gene="argR"
                     /locus_tag="ACHA34_002223"
     CDS             complement(7204..7659)
                     /gene="argR"
                     /locus_tag="ACHA34_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8792830.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7757..8374)
                     /locus_tag="ACHA34_002224"
     CDS             complement(7757..8374)
                     /locus_tag="ACHA34_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8792831.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8595..9968
                     /locus_tag="ACHA34_002225"
     CDS             8595..9968
                     /locus_tag="ACHA34_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8792832.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10043..11155
                     /locus_tag="ACHA34_002226"
     CDS             10043..11155
                     /locus_tag="ACHA34_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8792833.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11227..12864)
                     /locus_tag="ACHA34_002227"
     CDS             complement(11227..12864)
                     /locus_tag="ACHA34_002227"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFM8792834.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13032..13640)
                     /locus_tag="ACHA34_002228"
     CDS             complement(13032..13640)
                     /locus_tag="ACHA34_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8792835.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3803 a         2913 c         2478 g         4755 t
ORIGIN      
        1 aattaagaaa ctaaaaaaat aatccaagaa acattgaacc tatggatcca aaagctaccc
       61 aaatactata gctaaccccc cgctaccttc aattttgcag ataaaacgct ttgggagaaa
      121 atttcaaaga caacaggcca tcctatcatg cccgattata aacaaaaagc tggctattcc
      181 ttttgctctg acaaatttca attgggaaag ttcacgaaac tagcaggtga agaagtttca
      241 actgttcgga tcaaagaatg cccgcttcaa atagaaacat tggttcaacg aatttcaact
      301 agacaagact ttgctattgt agaacgtgag atcgtagcta tttttgtaga agaagggatt
      361 ttacatgatc caactcatat caatgtggat aaatggcaac ctcttatcta taaattcaga
      421 gaatatacaa ctgccaacca gtcgttaact ttgaatttta gattccaaga attcaaagag
      481 tcataatgat aaacatcgtt aggtataaat aaaaaagact cgcagcacga gtaaaaaaca
      541 aatagaagct atacattaga aaggtcgact gtgaccctat agatgcagta tatgcagctt
      601 atgagcattt cttctgttct ctttgttttt gggatctgtg ctgaagtctt tttcgttttt
      661 tgccgctctt tctttgaaaa atcgattatg ttataccttc tccattacta attcaggcat
      721 ctgctgagta atgatttttc gcgcaaaaga aatatctgtt acccaaggtt cgatttgttc
      781 ttcagtcaag acaagcggca ttcggtcatg gattggtgcg actgtgcggt taggttcagt
      841 agtcattatg attgattctg ccattttttc atcctgatgg ctacgataaa aaccgccaat
      901 atacactatt ttttccttat ttgaaaacat aaatttgtct ttttcttcat tccactcata
      961 aaagccactc attgggaaaa cacaacgatt ttgcaaaaat ggttgatgaa aggtcttttt
     1021 ttcttctact gtctctgcac gggcattaat catcaattgt ccttttttaa atccggtaaa
     1081 tccccacttc atgatttccg gagtgaggat gttttcttcc tcttttctta acgtcacaac
     1141 aagatttgac ggaaaaactt ctccagctgc taattctgct gcctgcttcg gttgtttcgt
     1201 ctgggcttct cgataataat tctgaaggag ctctgactct agatcaaaaa aataacgacc
     1261 acacatccta atccctccca atcttttttt ctatcataca ctgcaacaag aagttcgcac
     1321 caacaaaacg ctttcaattt ttgtagaact aaaaaagttg tgacaagtga caaactctcc
     1381 agtgaatggt attctcctgc caactcctcg gtcacaagtc acaatattca acaaacatga
     1441 gaagctgctt tttgaaattg ttccttgtga ctcggagtga aacggcaggc ttacgacctc
     1501 tgggtaaacg gtgttcaaaa gccagtttct cggtattaag ccaaatttat gcaaaaatgt
     1561 agaaagccat tttcacaaaa tttttcttaa tactcgaaac taagcgactt tttcacaacc
     1621 tcttccttaa acggtgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
     1681 atatgagcag ctatttttat ttggctgttc ttattacttg atgcagggca gctttttcac
     1741 aacctctcca ctaaacggtg ttcaatcagc taaccttcgg tattagctca aaaaacggca
     1801 aaatatgagg aactattttg ccgtttgttt ctctaatact caggtcaaag cgctgatttc
     1861 acaacctcta atacacggtg ttccagaagt agcttcttcg aaaaataagc cgaaatttca
     1921 caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gaccacttct
     1981 gtcacaacct ctaattatta aagaaccttt tgcgaagtta acaatctttc tttgatcaat
     2041 tgaaaattct gcctaagtag tgatagatcc tgtgtgactt tcaacaataa gatacttccc
     2101 tggatttcag caatcaacgc tttggcttga cggtctgctt cttcatcgga cagtttttct
     2161 tcagaaagtg ccaggatatt ttgtttcgta tacttgaccc attgattgac taattctagt
     2221 agcaacagac ggtattcttc atccactgta ctcaactcaa taatcaaatt acctacagga
     2281 cagccatgat aagtgatctg tgtctcatga tcagcgaaaa tccagtccaa catttcttct
     2341 aatgcttctt ctggctgttt tccagcattt aagatgtctt cgaacaattc tgtctgccat
     2401 acgtccatat gcgcatggat caccgctaga caaacttctt tttttgaaga aaagtaataa
     2461 taaaattgac cttttcctgt atcagaagct gctagaatat ctcggattga cgtttcatga
     2521 tagccttttt tataaataag atctttggtc gcttccaaga tcttttcctt tgttttcaat
     2581 gatattctcc tttttcattt ctcatagtcc tatattatac agtaaattca tcattttttc
     2641 ttcttaatcg tttaaaaaag actgtgccaa attatctgac acagccctca ctataaaact
     2701 gttctcatat agctagatgt tcattgattc aagaactgcg aggacaaaag caattcctat
     2761 cttatgatag ttgctctaat acttcctctg ttgtcatcac ttgatctgca ataaagccaa
     2821 agttcgtcaa cgctgcatcc attgcttctt ggccaggagc accaattgca tctgtcgcta
     2881 ccacaacatt gaatccactc tctaccaatt cacgcatatg ggaatcgcta cacaagtttc
     2941 cgttcattcc accaagaatt actgtgtcga ttccattttt tctcaattgg agcgccaaat
     3001 cattgctttc tggtccaaag attttatgag gagagctgat gatcgtattt tcatccaaat
     3061 aaggtttcaa ttcttcgata aaatcggctc ctgaaccttc ttccactgat tcatattgac
     3121 tttttctagc aaacatctgc tgctcaagca tcatatgttc tcctgcaccg ccaaattgcc
     3181 atttatggtc atgtgggtaa aaataatgag gagaaataaa gagtttgtat cctttttctt
     3241 ttgcttgttt taccatttca atcatatttt cgatcgtatt gtatttttct aagatatctt
     3301 ttgtcaaacc aaagccaccg cctgctggtt tcaagaactc gacttgcggg tcagtgagaa
     3361 ctactgctgt atttttatta ttgaacatgg tggattctcc attcgttatt tattttaata
     3421 catcatttag agcttcactc attgtcggat gagaatagat ttgatctctc aataacgtat
     3481 aaggaagttt tccattcata gctagcgaaa tcaagttgat gacttcatga gaatcttccg
     3541 catagatact tgcccctaga atcaagtcag tttcagggtc gactaaaact ttgaatacac
     3601 ctttaggatc ttctaatact tgtgctttag ggacaccgct tgtcatatac ttgaataatt
     3661 tgtaattgac gccttgtgct ttggcttgtt tttctgttaa acctacattg gataaagtcg
     3721 gagtgataaa tacactgtaa ggcacagctt tccggttatt cgttgttcgt ttattctcgc
     3781 ccttcaactg atccataatg attcgataat catctagaga gatataagtg aactgcaatc
     3841 cgcctttgac gtctccaatc gcccaaatgt tgtctgcatc tgtacgaagg tattcatcca
     3901 cagcgattgc tccgcgatct gttagtttaa catctgtatt ttctaatcca agtgatgctg
     3961 tacttggttt tcttccagtt gcagccaaaa cttttgacgc ttggattgtt acttcttggc
     4021 catcttctgt aaaggtgacg gcagctgttg attcttggtc tgctacttga tctacagata
     4081 cacctagatg gaattcgatt cccgcatttg tcatgtcttc taaaatcatt tgcgcgatat
     4141 cttcatcttc acgtggaaga aattcagaat gggcatcaag aacgacaact ttagatccgt
     4201 attcattgaa cattgatgca aattccaagc caatatatcc agccccgatg atcactaatt
     4261 tttccggtaa ttgttcctga tccatcgctt gcgtagaatc aatcaagtag cgacttgtat
     4321 tcaatccttt gattggtaaa atgattggtt cagaaccagt attgatgaaa atccgttcgc
     4381 cttcaacaat cactttttca ccattattcg tcacttcgat ttgatggtta gaaagaaaac
     4441 gtgctgtacc gtccatcact gtcccagttg cctcatcgct gatcatatga taatttttgg
     4501 cgcgaagcat gcccgtcaat ttttctttcc gagcaacagc ctctgtaaag tcgattcctc
     4561 tttctccatt gaaaatcaat gatttagaag gaatacagcc gatgttgata catgttccgc
     4621 catacatacg tgttgacttt tcaacgacta aaaccgattc accttttgaa gctaacgcct
     4681 tcgctaaagt cttaccgccc tttccaaaac cgatcacaat attttgatat ttttccatca
     4741 tttttccctc gcttcttttt tacctaccga ttggtacaaa aatatgataa ccgataattg
     4801 tctattctcc aaatcatctg ctttatttgt caaaaaaaac cagacaaaaa gcggtccttt
     4861 cacgttttta gtctggttcg atttccttta ttcttctaaa caatcgttac ttctacatcg
     4921 atatttcctc tagttgcttt ggagtaaggg cagaattcat gcgcatcttc tgcaattttt
     4981 tgggcttctt cttcgctgat gccatcgata tgcacttcta atactacacc gatctgatag
     5041 ccgccatcat ctaaattata aagagagaca cgtgctttta ctgtggactc tgctgtcatt
     5101 cctgcttctt tgatcatatg ttgtaccgcc ccattgaaac aagaagcata acctgctgca
     5161 aataattgtt ctggatttgt tccttctgca tggattcctg gaggagtgat tttcatatgc
     5221 atcgagccat ttggtgcctc gacttctcca ttgcgcccgc catggttgat cattgctgtt
     5281 tcgtatgctt ttttcattgt actgctcctt tcgattgacc atttgctact tggctataat
     5341 tatagtgtat gacaagtaag gcgctttcta caaaaattct gcttattcga ataccagaat
     5401 gccgtcttct actatttttc caaaaaacaa aaaaatacta ctcgtgaaca acagcgtcat
     5461 atttgttcca aaataaatag caatcttttc tacataatct tttatatgat tgattttaag
     5521 atacatatat accagaaagt tgaccccata aaccaatact gttaaaaaaa taaccatatt
     5581 cgttaccatt ttttcctcct tttgctggtc tccctaccag cactcgattt aaccatacca
     5641 ctttttttgc cgtccaagca actagccttt caaaaagact ggtttattct gctgttaatg
     5701 attcaagatc agaaaaatgt ccgaataatt ttgctaattt tactaacgcc aacttgttct
     5761 ttccgaattc atcattttct atctgtaata ctagtaacgg ctcatgtaag cttaaacgga
     5821 tcaaaaacca gccgtttccg tatggctctt ctagatcata tcgaatacct tcttcatttt
     5881 ccgggtcgaa gttcatccct ttttcttctt ccaacagtcg cttcatttct tctaatatct
     5941 tgtttccttg ttcgcgtggt tcatcgctga acaattttaa tcggatttct tgtacttcta
     6001 cgggctgttt caactctttg atttgttcat ctaatgtgat accttttctt tttaattctg
     6061 gcaaaagcat caggatcttt gcgatgacat atgcaccatc atccagaaaa tagttttcct
     6121 tgaatgcagc gtgtccgctt gtttcgatgg ctagctgtgt atctttccca agacggttaa
     6181 gttctaatgc tttattgatt acatttcgat agccggaaat ataacgaact tggatgcctc
     6241 ctttttcttc aatgaatctt tgtagatgag aagaagtagg ggaattcgtc acgattcttg
     6301 ttcctggatg ctcttttaat acgattgtac ttaatactgc gatcaaatta tttcgattga
     6361 tcactgttcc agaagcactg acaactgcag aacgatccac atctgtatcg aaaatgatcc
     6421 caagatctgc ttcatttgct aaaacagcct gcttgatact ttccatcgct tctttattat
     6481 caggatttgg gatatgatta ggaaatcggc catctggttc caaaaattgg gaacctgttg
     6541 aatccgcacc taagctattc aatacttttt cagcaaagaa tccaccagca ccatttccgg
     6601 catcaacaat gattttccaa ccatttagtg gtttgctttc tagtgttcca atcccctttt
     6661 taatttttgt gactaaatct tgcgcataag gtgtcaaaag gtctgctttt tctacgcttc
     6721 cagtttgttc gctttttatt ttttgctgct gtgataggat aaaagcaata tcttcttttt
     6781 ctgctcctcc ttctgaggta aagaatttta ttccattata ataataggga agatggctag
     6841 cagtcagcat aaccgcagca tcgcacgaga actgttcaaa ttgagtggac ataaacatag
     6901 caggcgtagt agctaattcg aaatcgatga catgtatccc ttgttctttc agactttgga
     6961 tcaacgcctc tttcagtgct ggtccgctta tcctactgtc atgaccaatc ccaatcgtcc
     7021 attttttatt tggatcggtt gatttcttct ttttctttat ccagtttgct agtccccagc
     7081 cgatcttttt taccatgtct gtcgtcaagt ttacttcata tccttcaact ggtaatgcga
     7141 ttcctcgaat atccgatcca ttttgcagcg ctttcatatc tttcatataa aagtcctcct
     7201 catttattcc ctcatttttt ccagcttttc tgcaaatcgc tgtgcttctt cttctgagcg
     7261 gcaaataata atgaatgtat ctacaccggc catcgttccg acaacttcag ggtaagccac
     7321 ttcatctagc caattcgtta ccacatcggc accatttcgt tcagtcaata cgataaccat
     7381 gaactgaaca gactggatac gcaaaacctc gcgtctaatt gctgtacgca aacgttcttc
     7441 atttaaagaa acagatggct ggttaaataa tgtatagcgt actattttgt tttcattttg
     7501 actttttact aacttcattt ctcgaatgtc tcgagaaatc gttgcttgag tcacatcggc
     7561 gcctgcttca tgcagccgcg caagcagctc ttcctgtgtc ccaataggtt gttctgtaat
     7621 gatctggcgg atcagtgctt gtcgctgttt ctttttcata ttagacacct ctcaatgttg
     7681 attatagcaa aagataagta atatttcttc atattttggt attttttact tttatttttt
     7741 gttttcattc aggttgctaa cctttttcta ttttaacacg caaaatcgtc ttcatttttt
     7801 caggtgctga ttttcttgga tcgctgatgt aaatctcttt gtgttcttta gaagctcttt
     7861 catagccttc ttttgcgatg aatgcttcta atttctcaaa cgttgctggt tcttcggcat
     7921 agggcccgac gtgtaagatt tgggcacaaa gaccttcttc catctttttg aatactagtt
     7981 gttctttcaa gtcttccgga agtttgctag aacgctgaat ggcttcttgt gctatcgctt
     8041 ctgtaacaaa gtcaggctgt cggatcatca attgataaga aaaatgttct tttttcatca
     8101 ctggttcatg caaatacttt tcttgcagtc cccattgtcc ttccagtgga tagacagtat
     8161 acatctgata atcaggaatc agccaatttt ttttaggcga catgcgtatt gcatagctga
     8221 ctgcatatag acaggctact cttcgtttaa agtcttcctg attcgggtcg ccgatccctc
     8281 tgatgctaaa gaatggtttt ttaggaacct ctagtatttg cgggcttttt ttcatactat
     8341 agctcttgtc tttacgccat tcaatttttt ccatttttct tcctccgcga aattccattt
     8401 agttatttat tatcatacct ctttttgagt gacttggcta atcgtgttga cactattttt
     8461 cagtttgtgc tagaataagt gaagtaaata aatcgtacag ataacacgtt tcggcgtgtc
     8521 gagagagagg cggtttctat ttttataata gaaatcactc tcttttttta cgaaattttt
     8581 tagaggtgat gtaaatgaca aaacaaaaac agctgctttt agtacctgca gtaattgcaa
     8641 cagggatgat gtcttttgcg ggcgtattga tcgagacggc catgaatgta actttcccaa
     8701 ctcttatcag acagtttgat ttaacgactg cacaagtcca atgggtaaca acgatttatc
     8761 ttttgatgat ttcgatcatt gttccgctgt ctacttattt gaatcgcaat tttagtctca
     8821 aaaaactctt tttagcttca aatctgcttt tcttggcagg tataatgatc gatttcttct
     8881 ctcctacctt catggtccta ttgtttggtc gattgctcca aggtgcagcg acaggtatcg
     8941 ctcttccgtt gatgttccat attatcttga catttgcccc cctcaatcga cgaggcgcga
     9001 tgatgggtgt cggtactttg acaacggcca ttgctccagc aattggcccc acttatggag
     9061 gcattatgac ttcacatttt acatggaatc atatcttcct gtttcttgtg cccgttttga
     9121 ttctttcttt gattatcggc tggtttgcta ttcccgagat ggcagtacaa aaaagtggtt
     9181 atttggattg gataagcgtt ttaggaattt gtcttttgtt tagtggaagt ctaacatttt
     9241 tgagtacgtt gggaacagtt tccagctgga ttgctttaat catcggcggt attggctggt
     9301 tcctttttta ctatcgaagt aaaaaagtgg agcatccact gatccgactg tctattcttt
     9361 caaataaaac attccgcttg tttttgattg gcttcttagt ttatcagttt ttattacttg
     9421 gtgtatcttt tgtcttgccg aatttccttc agatcgttca aggaagtagt ccttttgtcg
     9481 ctggattagc gatgcttccc ggcgcagcgg ttggtgcttt gctttctcct ttttccggga
     9541 aaatgctgga tcaatatggt ccgaagaagc caatttttgc tgggctgatt ctttctttga
     9601 ttggttggct agcattattc ctactcatag gacacgcttc agtgggtgta ttgattgctt
     9661 gtcatgtgtt ttatatgatc ggtatcggat tatcttacag caatatgatg accaccggaa
     9721 tgaatgcact gaatgaagaa ctacaaggag acgggaacgc aatgttcaat accttgcagc
     9781 agttttctgg cgctgtggct acttcattag tagcggtcat tatcaattac gtccagcatc
     9841 atacttccca taactatgaa gtgtctacta cattaggatc aaaagcagca ttaggcgtct
     9901 tgttgctgtt gcttctcgta agctttgctc gattcgccta ttatctgttc tttgctaaaa
     9961 aagcgtagat tccatatacg gcaggtgttg catctgccgt ttttttgtgt gaaaatggtt
    10021 gtttgtaaaa ggaggctttt ttatggaaaa aattgccatt atcggtggcg ggatcattgg
    10081 tatgactcta gccagttact tagatacatc aaaattcaat gttgctttat ttgatgaagg
    10141 gacaggacag gcaactaaag ctagcgctgg gattatctcc ccttggttgt cgaaacgcag
    10201 aaacaaacaa tggtatcgtc tggctaaaga cggcgctgct tttttcccta aacttataaa
    10261 agatttccaa cttgatcaaa cgatttacgc acaatcaggg acacttttgc ttcgacctga
    10321 aaaagattta gctgaattgg ctgttcttgc cgagaaacga aaagaagaag cgccagaaat
    10381 tggaaacatc gaatggcttt cacctgaaca gacatctcaa gcacttccgt tattgaaacc
    10441 agcagtttca ctgcgtatca gtggtggtgg tcgtctggat ggaaaagcat atttagaaca
    10501 tcttggtcag cgtttagcac aaaaacaaat ggagcagatc caggcaaaag tcactttgca
    10561 taagaagagc gattcttggc tcatcaaggg accgtttgga gaatacacag ctgatcgtgt
    10621 aattttgaca cctggcccgg cacttaagcc tttattacaa gaattaggct atgctgcaga
    10681 tatacgtccg caaaaagggc agctcttagc atttcagaca tcttataaga tgagtcaaga
    10741 ctggcctgtt gctatgcttg acggagaagc tgatttcatt cctttcatgg atggaaagat
    10801 ccttttaggt gctacccatg aaaacgatca aggctgggat ttggctccta ctagcgaagc
    10861 ttataaggga ttgactaaga gtacagaaca gttcttgact gatccagatc agataaaaaa
    10921 ctggccatac gagttccgtg taggtacccg tgcttatacg tcagattttt ccccattttt
    10981 cggcgtactg ccagatgatc caagcttact ggctgctagc ggtctaggct cttctggctt
    11041 gaccacaggt ccttatatcg gctatctatt agccaacttc ttaaatactg gtcattggtc
    11101 tacggaagat tatcaaaagg acatctccac ctatattacg aaaaatccat catagaaaaa
    11161 cagctttttc aagtaaggat cccgagcgat attgcgcatg gattacttct tgaaaaagct
    11221 gttattttag ttcaatttct gaatcaaatt ctccaaactt tcttttgctt catctggcaa
    11281 gagatctttt cctatctgag cttcaatgaa tcgtaaacga atatcaagtc gttgtttcac
    11341 cagattttta taggacgtat cctcaaaaga aacaggaaaa tctggtatag gtaaatagtg
    11401 gatgtcttca aatggaccaa acgacttgaa ttctgccgtt tgttgcacga ttctttcaac
    11461 gattgacaat gtcgtttcag gtttgatttt tttatcatcg aaatatccag tattcaggac
    11521 atagcaggct gtttgctgtt tcaaaaacaa ctctcgaaaa gcttgatagt cttccctaag
    11581 aggatagaca cggaaaggat tagcaaatgg ttcaatgact agctcatcta gctgtctttt
    11641 tgtagtgata ttttccgcgt ctgatcgttt tgttgctaat gttgcaccaa aagacgctgc
    11701 taaaactggg tcattgatct tgataattgg cggaagcgaa tcatctttca tgatccaaaa
    11761 aacagcatcg atcttttcgc taagatgatc gactcggtcg ggtgtcgcat aacgtgattt
    11821 gactgtccgc ccatttccgt ttcgtatatc tcctgttact aggacttttt ctcctttact
    11881 tgtacatgtc acgcccacat tctgacaagt aaggaaagaa gaaatggccg gatcatcaga
    11941 cggatagtct tgtgttttat caaagtaaga aggttccaag gcagtagcaa aaccattttt
    12001 tcgagaaata acgaaagcat cgtcatgcaa gatcgaaaca gagtgacctt ccgtatgttt
    12061 tgctaaggta atcgttgatt ttccagaacc ggacaaccca aatacagcca tcgtataggt
    12121 ctggtcatta ctagcatatt gtttcatccc accgtggcag gcaataaagc cattgcgatt
    12181 tcccaatgcc caagcaagcg tcaaagtcgc tttttttaat tctccaaagt aacgtaatcc
    12241 tagcacagcc gctgtattat ggagagcatc aaacaaaatg agtccatttg gaaatgtttc
    12301 atgtgtccat tcgggatcac aaaagatata aagatctccc tcatcaaagg aaacagactt
    12361 ttgataagtc tctaagatct tcgaattcgc tggctgaaaa ttcagcaaat aagaatacaa
    12421 attgttctca aatccttctg gaatcagcaa atgactttgc acagaaaatt cttcagatag
    12481 gccaacgatt acttttgtat ggtaaaactg gcgcttgcga ctttgataca cagcttctct
    12541 taaaattttc tcgtatttgc tttgttcctt gtctaagtcc tcaaagatcc gccgagcact
    12601 tgctgttctt cccacaatgc ttccatcatt gaatactagt gtcctaacat cagctggtaa
    12661 atctagttct tcagcatgtg agattgttaa atctgtgatg atcgttcctg gactcccttt
    12721 tgctagttga taagctgtct tcctgtcaaa aacttcatga acattattac cgtaaaatgt
    12781 cgtttctaca atcgatcgaa tactggataa cagtggattt ccacttgtga tttcgtttaa
    12841 ttcgagactc tcttttgtac tcatggctcc tcctcattcc ctaaacaaaa aaacgttttc
    12901 tttattatac attattcgag tttttatttg tactgctgcg ctgtgggaag caaacgagtt
    12961 atttttcaca caaaaaaacc gagaacttga ataaacaagt ttctcggttt catcattttc
    13021 cttttatcgg cttagtacca gccgtttgct aaccagaatg ttttagcagc atcccatgaa
    13081 ccataacgag atgttacata ttgttctgct acacgttctt gattggcagc tgaatagtcg
    13141 ccgtttaagt atgaagcatc taattgataa cggccgatgt aacgtccgtt agtagctgtg
    13201 tatgaaccgc ctgattcttt ttgtgcgatc cattcttttg cagagcttga agttggcgct
    13261 gttgcagcag ctggggcagc aggagctact tcaactgttt caacaggtgc aacgtatgta
    13321 tgttcttgcg ttgctgtttc agttgtagat gctgtatttt ctgttgtttt gttttctgtt
    13381 gttgctgaag aatcatttga agttgggatt gttaattgtt caccaacata aatgcggttg
    13441 atgtttgcga tcgaattttt ttcagcaatt gcatcgatca ttgagttgtc tcctgcaaat
    13501 ttttgtgaga tcttagataa tgaatcacct gattttactg tataaacttc atcagcgtga
    13561 gcatttgtag ttcctacaaa cattgcagta gcaccagcag caaaagttgt acctaaaaca
    13621 attttcttaa atgaattcat attaaactcc tttttttctc cgttttttat tctttcgacg
    13681 ctattaactt tatcatttag aaatatttca taggtaaaag aaacatgaca tttaatgaca
    13741 aaataacgaa ttatttaata tttgtaacat ttaaacaaat atttttttga tacaaaacca
    13801 taaaagcctt gttgtataag caaagtaatc gtttttctat gtttttttat ttttgaatga
    13861 aaaacgcaga ttctgtaaca ttcactgcaa taatccataa aaaaaagcaa aaaaattttt
    13921 gatttttatc aaattctctt aaaaaaatg
//
LOCUS       DAYYFU010000062        13917 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000062 DAYYFU010000000
VERSION     DAYYFU010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.065"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACHA34_002229"
     CDS             131..505
                     /locus_tag="ACHA34_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8792836.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACHA34_002230"
     CDS             complement(619..1248)
                     /locus_tag="ACHA34_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8792837.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACHA34_002231"
     tRNA            complement(1382..1454)
                     /locus_tag="ACHA34_002231"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="ACHA34_002232"
     tRNA            1668..1741
                     /locus_tag="ACHA34_002232"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="ACHA34_002233"
     CDS             complement(1879..2043)
                     /locus_tag="ACHA34_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792838.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="ACHA34_002234"
     CDS             2338..3666
                     /locus_tag="ACHA34_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFM8792839.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="ACHA34_002235"
     CDS             3868..4701
                     /locus_tag="ACHA34_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFM8792840.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="ACHA34_002236"
     CDS             4717..5454
                     /locus_tag="ACHA34_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFM8792841.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="ACHA34_002237"
     CDS             complement(5609..6577)
                     /locus_tag="ACHA34_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFM8792842.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="ACHA34_002238"
     CDS             6809..7642
                     /locus_tag="ACHA34_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFM8792843.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="ACHA34_002239"
     CDS             7676..8515
                     /locus_tag="ACHA34_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8792844.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="ACHA34_002240"
     CDS             8541..9101
                     /locus_tag="ACHA34_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8792845.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="ACHA34_002241"
     CDS             9300..11021
                     /locus_tag="ACHA34_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFM8792846.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="ACHA34_002242"
     CDS             11186..12328
                     /locus_tag="ACHA34_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8792847.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="ACHA34_002243"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="ACHA34_002243"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFM8792848.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4751 a         2298 c         2833 g         4035 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DAYYFU010000063        13810 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000063 DAYYFU010000000
VERSION     DAYYFU010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.066"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(221..1423)
                     /locus_tag="ACHA34_002244"
     CDS             complement(221..1423)
                     /locus_tag="ACHA34_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8792849.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="ACHA34_002245"
     CDS             complement(1426..1620)
                     /locus_tag="ACHA34_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8792850.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="ACHA34_002246"
     CDS             complement(1667..2515)
                     /locus_tag="ACHA34_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFM8792851.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="ACHA34_002247"
     CDS             complement(2720..3070)
                     /locus_tag="ACHA34_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792852.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3248..4111
                     /locus_tag="ACHA34_002248"
     CDS             3248..4111
                     /locus_tag="ACHA34_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8792853.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="ACHA34_002249"
     CDS             4735..5727
                     /locus_tag="ACHA34_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8792854.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="ACHA34_002250"
     CDS             5743..6627
                     /locus_tag="ACHA34_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8792855.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="ACHA34_002251"
     CDS             6676..8283
                     /locus_tag="ACHA34_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8792856.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="ACHA34_002252"
     CDS             8284..9768
                     /locus_tag="ACHA34_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFM8792857.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="ACHA34_002253"
     CDS             9945..10937
                     /locus_tag="ACHA34_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8792858.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="ACHA34_002254"
     CDS             10989..11858
                     /locus_tag="ACHA34_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8792859.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..>13810)
                     /gene="guaA"
                     /locus_tag="ACHA34_002255"
     CDS             complement(12278..>13810)
                     /gene="guaA"
                     /locus_tag="ACHA34_002255"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8792860.1"
                     /translation="KIIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPK
                     GIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGK
                     AELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKM
                     YGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLG
                     LSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAK
                     ERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGT
                     ETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPG
                     LGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRT
                     YDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVE
                     WE"
BASE COUNT         4231 a         2337 c         2851 g         4391 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt
//
LOCUS       DAYYFU010000064        13730 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000064 DAYYFU010000000
VERSION     DAYYFU010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.067"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1451
                     /locus_tag="ACHA34_002256"
     CDS             <1..1451
                     /locus_tag="ACHA34_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8792861.1"
                     /translation="KKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTEM
                     SNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSNA
                     ESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFST
                     PQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQ
                     AILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENY
                     EESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELI
                     KTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHA
                     VKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTG
                     NSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1800..2432
                     /locus_tag="ACHA34_002257"
     CDS             1800..2432
                     /locus_tag="ACHA34_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFM8792862.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3020..4459
                     /locus_tag="ACHA34_002258"
     CDS             3020..4459
                     /locus_tag="ACHA34_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8792863.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4754..4972
                     /locus_tag="ACHA34_002259"
                     /pseudo
     CDS             4754..4972
                     /locus_tag="ACHA34_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            5221..5436
                     /locus_tag="ACHA34_002260"
     CDS             5221..5436
                     /locus_tag="ACHA34_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792864.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5653..5909)
                     /locus_tag="ACHA34_002261"
                     /pseudo
     CDS             complement(5653..5909)
                     /locus_tag="ACHA34_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6096..7007)
                     /locus_tag="ACHA34_002262"
     CDS             complement(6096..7007)
                     /locus_tag="ACHA34_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8792865.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7026..8030)
                     /locus_tag="ACHA34_002263"
     CDS             complement(7026..8030)
                     /locus_tag="ACHA34_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM8792866.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8027..10150)
                     /locus_tag="ACHA34_002264"
     CDS             complement(8027..10150)
                     /locus_tag="ACHA34_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8792867.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10155..12602)
                     /locus_tag="ACHA34_002265"
     CDS             complement(10155..12602)
                     /locus_tag="ACHA34_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8792868.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12589..12978)
                     /locus_tag="ACHA34_002266"
     CDS             complement(12589..12978)
                     /locus_tag="ACHA34_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8792869.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13038..13541)
                     /locus_tag="ACHA34_002267"
     CDS             complement(13038..13541)
                     /locus_tag="ACHA34_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8792870.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
BASE COUNT         4220 a         2710 c         2509 g         4291 t
ORIGIN      
        1 tgaaaaaatc tttaaaactt gcatcagcag ttatattaat gtcccaactc cttatagcaa
       61 cacctttcca tgtgttggca agcgaaaatt caacatacaa aattgcaaaa gaaagaaccg
      121 agacgactga aatgagtaat tccgatgtaa cagcagagag tagtgaactt ttgacaactt
      181 catcaagtga aagtagtacg acttcaaatg ccgagtcttc aacaacttca tcaagtgaaa
      241 gtagtacgac ttcaaatgcc gagtcttcaa caacttcatc aagtgaaagt agtacgactt
      301 caaatgccga gtcttcaaca acttcgtcaa gtgaaagtag tacgatttca agtaccgaat
      361 cttcaacaac ttcaagtagt acaagtgaaa taacttcttc gaaagtgaca aaaccaaacc
      421 atatagaatc ttcaaaaaag gaagcgccaa aaacggaagg acaaacaaaa actttttcga
      481 caccacagca aatagaatca acagaatctt tccaatcaca accaatacta tctggtaatg
      541 aagaaattca ttttgacaaa aatgaatctg tagaaaattt tattatgaaa attggtgagt
      601 ctgcgagaaa aattggtcag aaatatgatt tatatgcttc tgttatgatt gcacaagcga
      661 tcttagaatc tgccagtggt caaagccagt tagctcaggc accgaattac aatttatttg
      721 ggataaaagg aacttataat ggaaactttg tcattatggt aacaaacgaa gatttgggta
      781 atggaacgct ttatacgacc caatcaaaat ttagagtgta tgaaaactat gaggaaagct
      841 ttgaagatta tgccaaatta ttaactaagg gaattagtgg aaataaagat ttttatgcag
      901 gagcattaaa agctaatagt aaaacatatc gagaagcaac taaattcctt acaggacggt
      961 atgcaacaga tacacaatac tatttgaagc taaatgaatt aattaaaacc tacgatttaa
     1021 ccaactatga taaagaagtt aaaggtccac aaatgagtaa gactggatac caagtgccta
     1081 ttcataatta ttcaataacc agtttgtttg gaaatcgtgg aaatgaattt caccgaggac
     1141 ttgatatggc agctacacag ggtgagccga ttcatgcagt taaagctggt aaggttgtta
     1201 aagcagagtt tcactactct tggggaaatt acgtggtgat acaacacgaa gatggatcaa
     1261 ccgcgttata tgctcaccag caacaatacc tagtaaaaac tggagataat gttttgcaag
     1321 gacaaataat tggctatgta ggaagtactg ggaatagtac tggcccacac cttcatttgg
     1381 aaatttgcaa agatagtagt ctttctcaga gcatgttagt tgatccgaaa accttattat
     1441 tcggagacta aaaaaggtaa tcaatctgac aaagtaacaa gaaaatggtt ggattcttag
     1501 acctttgatc aggatattga gactggaatt gatatctcga ttacgatggg tttgacaaat
     1561 aaaacaagtc cattatcgaa tatcgagtgt attattgccg tctttatgct catattttga
     1621 gcaggtttca ctagatgaaa acaatttatc tatcttgagg atttcgtgta cgtatcgttc
     1681 agtcttgtat tgtaaattag tcataaaatc agactaagaa aaattggaaa tatttttttc
     1741 tgatttatta aaagttaatt gaaattatat ggtacttctt tcttgggagg gtaggtgcag
     1801 tgaaggaaag atatggtcac cttactgttg ttgaggagat tactcataat ggatataaaa
     1861 aatatcgttg cctatgtgac tgtgggaatg aaacgattgt atatcgatca aacttactat
     1921 caggtagaac caaaagttgt ggatgtggaa attataaaaa taggcataaa tataaaaatt
     1981 tgacaaataa aacttttggt aagttagttg caatcaagcc aactaaactg cgaagtgaag
     2041 gaactgttgt ttgggaatgt caatgtctgt gtgggagaaa aaagttagta tcggcaaagc
     2101 gacttctaaa aggtgatgta aaaagctgtg gatgtttgaa atcaaaaaag catcacattg
     2161 aaaataaaaa attcggatta cttactgcaa agtatccttt ggacaataac tgttcatgga
     2221 aaacaaaatg ggtttgtcat tgtgagtgtg gcagtatgtg tgagataagt tatagtaact
     2281 tattttttgg acatactcaa agttgtggat gtttaaaaag gcaagaaaca aggacatgtg
     2341 ttgaagggac agtaatagag tgtctaacgt caaaattacc gaaaaataat acaagtggtg
     2401 ttaagggagt ttatcgttct aaagagaaat agattgcgta tataacttta aaaaaaaaga
     2461 ggtactattt aggatcgtat gattctatta agagtgcagc tcaagctaga aaagatgctg
     2521 aaaataaatt gtttcaacct ttgattgaca aatatcgagg taatcattaa ttattgtgga
     2581 taataattaa cagtgatata taaacatcta taatatatta ttcatactat tgatatttag
     2641 tataatccta tggaattgaa tatcatagca atgtatttga aaagcaaatt ggaaaacaaa
     2701 taggtgcaac accataaata tgaaaaaatt gggaaaaagt agaatggaaa tttgcttacg
     2761 tctcacgaga tagctcacac tattaatctg aagaacaatg aagtcgactt ttagaaattg
     2821 ctagaagaaa gtaaagcgaa ttatcactag ttactataga gtaagttcaa ataaagcatc
     2881 tccaacttac atggttacat gaggatatca aatcgaaatt actcaagctt cctatggcta
     2941 gagtcgcgag aagttgttgg gcagtagaca taggttatat tgttcttagc tacacgatca
     3001 ttagaaagga aatcctatta tgtatagtta tcaagaagat gcaaaagtcc aaaaaaatcg
     3061 ctggtggatt ttgattgcta tatctatgtt taccttcatg tccacattgg atggcagtat
     3121 tgtcaatatt gcactaccta ccatctcaga tgatatgggc ataccaatga atcaagcgga
     3181 atgggtagtg tccgtttatc tgatgattgt ctgttcttgt ttgttgctgt ttggtaaaat
     3241 tggcgatagc cgtgggaaaa tcaaagtatt tcggattggt acgctgatct ttgtcttggg
     3301 atcgctgttg tgtgggttca atcattcgct aggtatttta ttgttcgcac gggtggtaca
     3361 agccattggt gctagtatga ctatggctac aaatgccgga atcatcacgg aagtctttcc
     3421 catacatgaa cgtggtcggg cattgggagc aataggtgcg tttgtttcat taggctcgat
     3481 tgctggacca ggtcttggcg gcttgatttt gtcccaattt tcttgggcgt acattttttg
     3541 gatcaatgta ccggtgggaa tcgtaacgat gttgattgct gaaaaattct taccaaaaga
     3601 tatcaccatg agcggccata caatcgatgg agggggcttt gctgccattg cggctacgat
     3661 tatcagcttt tttggagcaa tctttttggg gcaagaaagt ggctttttcc atccagtccc
     3721 ccttgtactg ttcgtaattt cagtgattgc ctttgtcagc tttttaaaag ttgaaaagaa
     3781 agcagcctct ccattgattt ctttcggtat ttttaagaac aagattttta ccatgagctt
     3841 gattacagcc ttgttgattt tttcttccaa cttctttgtc aatgtagtga ttccttttta
     3901 tctgcaaaat gctcgcgggt tgagtcctag ctatgctggg ctgttgatga tggtctttcc
     3961 tttgatgatg gtcgtcgggg cgcctttaag cggctacctg accgaccggg ttggcccgga
     4021 gattttggta ttagtaggct tgggcttatt gtcggtaacc caggtcatgt atatgttttt
     4081 gtcagtggac tctcctttgt ggctatacat tgtcgctact gccatcatgg gtgccggcaa
     4141 tgccttgttc cagtccccca ataatacgat ggttatgagc tcggtcacta tcgaaaactt
     4201 gggactggca ggctcgatga attcctttgc ccgaaatttg gggatggtca gcgggatttc
     4261 gctttctacg acggtcttat atcaagcgat gtctgctaag atgggccagc aagtaactaa
     4321 ctatatcgca aatcggccgg atgtcttttt atatggaatg aagattacct ttttaggcag
     4381 ctttgttttg tgttttgttg cctttttcct gactatctgg cgtatcaaaa aacaaggagg
     4441 tattcagaaa aatgtctgaa aaaaccactg ttgaagaatt tttggaggcc tgcgtatggg
     4501 tgagtgggat ttccatggat gatggaagat gaagtgaact tctaaaagcc gaaaaactca
     4561 agtattatct tcaaaatact agtgctcaat gagatgcttg atttgatcac aagaagactg
     4621 cgaaagccgc tggcaaccgc aagctaggta tcccaccgat ggactacgtt ttagaggcta
     4681 ctggttatga atacggagcc aacgctctca tctgcataag aatacatcac ccagattatg
     4741 taaagatttt cgatacttct gtcaaaaaat cattaagata gataaatgat ttccgtctag
     4801 tcaaattggt tcagaatgta gacacacaga tggtaagaag tcactcacaa ttcgagaatg
     4861 gacttgtcct atttgccgta tacatcataa tcgaaacgtc aacgatagta tcaatatttt
     4921 gaccaagggc tatgaataca agcattgcct tagaaaacat caagaacctt aggaacaacg
     4981 gggatagctt ggttaataag agataccgct gtttaggctt gcaagtatac tcttttatca
     5041 agaattccgt ggctttagtc atgagaggct taatactatg aaaattttct ttagcatttt
     5101 tgatttaggt tccttttcta attataataa gatgaccact acagtaacct actgaaatat
     5161 ataaacagcc actgtagtac cattttcaaa gtagtctatg agaggagaat cttcattctt
     5221 atgaacgctg gaattgcgga tatgaattta ataaaaaaaa cccttaatga ttttacttcc
     5281 aattcaatat caaaaggtac tggtatcaat ttaagcacaa taaaaaagct gaaaagtggt
     5341 gaaagatcag tggaaaagct gaatctttta gatgctataa aaataacgga atttgctatg
     5401 aaaaatggaa aagctgagat tgaaatatgg agataattat cgtctagtga attacttgcc
     5461 acttagctga atttaacgat ccttggtgct tgaaggggga gattcttggg aatagagcat
     5521 acttgcaagc ctatgtcttt actaacaacg atatctctta ttaaccaagc tatccccgta
     5581 gttctaaggc ttttactttt atctaagcaa taatcgtatt caaaggaaat ttttgaccct
     5641 tagaaagtct cattttatca gacgtgagct taggcattgc tgaattttgt taattcggtt
     5701 ttaggttgtg ctttgagcat aatatgaaag tattctttat cacgatttca ttcatctaag
     5761 gtaatgtgat gtgattctga aattctttca aaggcagttt ttgcataagc tgacattaca
     5821 tcatcaatga catgtttaca atgtttcact actaaaataa gggggtaacc aagaagaaaa
     5881 actgaatgat tatttgtatc taattccatg gtatatcaac catttcctat atagactgat
     5941 tatgacatgg attaaaataa aaaggcacta tgtcttaatt gtcggttttc gagttaccga
     6001 atggcattca tcacccacct atagaggatg ggcgacttct gcctgaaata agttacacta
     6061 cttataactt ctaataaaat tagattgagt cattattatt taacatcaac gattttccag
     6121 tttccatttt tttgtaatac taatttgtac tgaaagtagc tagtggtttt tgatacatta
     6181 tcaaggtatt ttatctttgt actcactaga aaattttttc cgttttgtag ataaactgga
     6241 ttctctaact ccgaaaactt taagtcaata ttgattggtc gcatcgcacc attttcaaca
     6301 taatactcca attctttttc agaagctgat ggataaagtt taaaaaatgt ttctaaaaaa
     6361 tcggtgattt cttccgttgt tttggcatca atttctgaat cgttttctac ttgttttgct
     6421 tcatattttg ccttgtctgg ctttccaatc attgttggca aactagttac taaatgattc
     6481 ctgttttcat cttcaaagat tgtcactcga tatgcagaag aaattgattg cgtctttttt
     6541 ccctctgtaa tatgttgctc cactaaaaat gaaacgacaa attcttccga gtgtttttca
     6601 acatctagaa tttttacaga ttgaacttcg gaactcgtgg gaatatctgc tcgaaccata
     6661 tcttggatta aagcgagacc gttttctgta aggtactgtt cgagattact cattctcttt
     6721 tcaattgctt ctttgttatt tttccatgaa aaatattctt tcgcaaaatc ctctgtaaaa
     6781 ctttcaacgc cagaagtatt cactaccttc tcttttataa ccactttttc atgagttgta
     6841 tgttgatcaa tggcggtgaa atttttataa acgccaaaag caagactaca agacaataaa
     6901 agccaaaggg caagcaccat ttttcgatgc ttacccacgg atacaactcg ttcttttttc
     6961 ttttttgggc tcttttcctt ttgattccgt tcaattttta tcttcattct ttttcctctt
     7021 ttctcttatt tgtatcggcc agctccgata agatgctgcc gccaatagtt ttccgttaga
     7081 ttcgtccacc ccactggatc tcctgcatga aacatgcgat tctcccctgc gtaaatgccc
     7141 acatgggtca cataagtacc ggcatcatag gttgaatgaa agaaaactaa atcacctggt
     7201 ttggcatctt ttagatcaat atgagtcatg gcatcatatt gatcctgagc cacccgagga
     7261 agtttcaggt caactttggc ataacaccat tgggtcaagc cactacaatc aaaccccgtt
     7321 tctggacttg agccaccaaa cacataggcc gttccttcat acttaaatgc ctcatcaaaa
     7381 atttcttgga ctattttgtc atcaaactga gttgtagtta ggtattgctt cactaataaa
     7441 cagtaaaaca tattgccata attataccgc cacccaccat tttccttaat cgcgattggg
     7501 ttggggtaat cgactttttg accacctgat ttttcctttg aaaaagattc agctaaagca
     7561 aaactatgct tcttcccgtt tttcccgaca tgatctagat acccgccacc atagttatag
     7621 gcctgaataa tactatcttg atcacaccct agttctttgg aacgcttaac caaagacgca
     7681 aaatagagac agccttgttt gatggatgcc tcggtactta atgaattagg tggtaatccg
     7741 gctgactcag aactttgcat aacgtcttcc attgtgccac cagattccac ttgaataatc
     7801 gccaaaagaa ttggaatata ctctgttata tccgattctt tggcatactt ttccaccatt
     7861 gcacggtgtt tcaatacttc ttctgagaca ctagttccgc caggagaagt gccagaacta
     7921 ccatttccct cactgtcttc accaaacaac aaacccacac acatcaaaat acccataaaa
     7981 gagatggtca gaaaagttaa tgaaataaca cccactttct tcaagttcat tttttctcac
     8041 ctgaccttga ttttggacga tggttcattc ttttttgtac tttttgattg gtctgctttg
     8101 gcagagtgga cctctgtttc acttgttgga aagaattttt cttacttgct gattggcttt
     8161 tcccttttga aatgatttct tgactagtta gttttcgttt gatttcggga ttcagatttg
     8221 ctctcacaat ccgctttggt gcttcttttt tcagcatcat atctttctga ggttgagctg
     8281 ctttatctat tggaatattc gcccttcttt ttccctgacg atttgttgct tcactcagta
     8341 tcttgcgttt ttcgtccatt ttcgcatttc ttcgttgtcg ttgtttttct cgcaaatcca
     8401 ctcgatccgc tttttcttgg aacagtccgc gcttgaattc ttctggtgct tttttcgact
     8461 tttcgattcc tttatggaga ttgtatttca aattcgttgg cgtattcctt acctgatcct
     8521 ttacttgttt agtcttatct ttcaccaaag cttgggtatc caaaactttg ccgatttttc
     8581 tcccagccaa attcattcgg gattgtttct tattatttga aactggattc tccttagtta
     8641 agtcagatag tatttcgtga tcgttcggta atcgtgaatt ctttcgtaga gaagaaccgg
     8701 atggcttaaa tttggatttt gattgttcct ttgccaactt atttccgact gctgcaccac
     8761 caccaaaagc caaagctcgt cccacatttc gattgagttg ccgcaatttt cgattcatta
     8821 acatttgcgg ttttcgcatg acgcgtcttc ccattgactg gctatcatta ctctggaggt
     8881 tgaacatact cataatgtcg cccaatttga aatagatacc ggcaaaggtc acaatttgca
     8941 gaaaagcaac catgaaaaat ggataggttg ccgaaatatt gaaaaacatc gtggagatgc
     9001 taaatgcagt cgtaatcacc aaagtaacac ccgctcgcat cataatcgta ttgaataaac
     9061 gaataatcgc cttctttcct aaactttcat aagtcggcaa catggacaat aagaaactaa
     9121 ttggcagaaa catcgcaaag ataatgaaga gaatttgcga gaacaacata atccctgtta
     9181 gaagaaaaac aaagaaggaa atgccaatat tgaacaatac caaaaagacc acggtcccta
     9241 aacggttcat ggtcttggta atactgagat tcgcattgtc attgtcttca atctctgcct
     9301 tgaccgcttc ttcccgatct ttgcctttgt tttcatctgg actcacagat agaatcttgt
     9361 tgacccggtc ttcaccaatt tcttctatgt tggaatcatc aaattgcagt agtaaccaag
     9421 gttgttggac ttgaatcgaa aacaaactat cccgaatcaa atccacacta tcttttcctt
     9481 ggctttctga attgggtaca acgatctcgg ttccaagggt aagggccgct tcactaacat
     9541 ctgagctaaa gtcattgatt tttgtgatat aggtaggcgc ataagcaatg aacgaacccg
     9601 atagtagaaa aatcatcaca aaattaatca cggctcgaac tgctttcgtg gtttctcgtt
     9661 ttaataatcc ggtatacgcc acatacaccc ccagagctaa aatcatcaat aatagaaacc
     9721 caaaataaaa tccggaggtc tggagtccat tttcagtaat accagccaat gtttggatat
     9781 tctttccaat actttcagcc gtatccgaaa tgaaatctaa tttgtaagcc tcttgaacca
     9841 cataacccgt ggcgttcgac aaatacaaac tcaccgtcca aatgaaatta gtaatggcat
     9901 aaagaccata catcacgctt ttccctaagc catcacccca attccaaggc aaccaatccc
     9961 aagagctatc cacaaagaaa tctagttgat agttgttcag tgaatatttg gaatatagat
    10021 ttcctgcttc aactgtatca tcaactagcc ctgtcgcctc ggctaccgta ccaactaaac
    10081 tcaatagaac tatcagagtg agtatgctaa taaaaacgat tcccacaatc cgaaaaagtt
    10141 tctttttcat cccctcactc cattctctct tgaacaggtg gacgggtatc aaaggcatgg
    10201 aagagttctt caaagactgg atgtacctga accacgccta cccgaccata caaatcctgc
    10261 atcaagcatt ggccattttc caaatctcgt aggcgttttt gattgctttc atcttctcga
    10321 tccaagccaa agaattccaa ggttttcttg atttcatgaa tatcggtact gcgaaacgca
    10381 aacttcaacc cgatattatt cttcaagcgt tcatccagta aatcatcact attttgcgtg
    10441 acaaagtaaa ccccggcatt catagcccgc ccagcacgaa cgagcttatt cgataatgtt
    10501 ttcccttgcg ctacttgaag aaaactccac gcctcatcta agtctacaat tttgaagatt
    10561 tctcgattac tatggataaa gtccaaggca aaagtggaaa tgacgattag catggaaaca
    10621 ctaagcaatt ccatggtggt atattcttca aaagtggttt ctgcatctgg taaaaccaaa
    10681 tccgctactt gaatgatatt taactgccta tctaaactaa tcgactgggt aatcgaacca
    10741 tctgagaaaa gcaaatgggc aaaatcataa tcggtaaagg actcgatgtg atccgcgata
    10801 ttttccgcaa ctggtgagcc ttcttttcga agctcattaa ttacttgtaa taatccccgt
    10861 tgattgcttt gggtaaccgt ccgaatggcc ttacgcaaca ctggaaactt ctccccatct
    10921 cgactagaaa ttcctgttaa aaaggtcaga atatcaattg ctagactttc agaatccttc
    10981 tttttctcca taatcacata aggatcaagt aatccttgat tttttggctt actagttaag
    11041 ttgagaatat tgatttcatc cgcaatttct ggcaaggttt ctttccagcc accccgttcc
    11101 ccttttggat cgacaatcac agcttgtcca ccaaaaagga cggcgtaata aactaagaga
    11161 ttgttactaa aggactttcc gccaccgagt gaaccgagga atgccgctgc tagcgcattg
    11221 gtaactgaat ccttgacccc ttgtgaggcc aatgccggtt tcaggtaaac attgcgcccc
    11281 gtatctacgt tataaccaaa gtaaatgcct tccaactccc ctaacatctg agtggcgcca
    11341 aaaccaagtc cggcgagaaa atcagacgta acatactgga tataatcatt catgtatcgc
    11401 ttactagaag gtaagaattc ttcatgtagc cccatcatat cgccaaaagg ccggactagt
    11461 tttacattgg tatcatcata aaaatctagc acttcatcac accggcgctt tagttcatct
    11521 actgattcgg cacttactcg aaccacatag ctcagtttgt acatggattc tttggactga
    11581 tccaaggtag tttccaattc atctacggaa tccaatgcat ctaagacatt ggaatttgtt
    11641 tcattgtcag attgataggc atggttgtcc aagtctttta attccttttt cttattgcga
    11701 acggtagcaa gtgccttttt gttcgtcaca atctccacat tcatactagt gtcaattggg
    11761 aatgtaaatt gttgttgctg gtaatagaaa agttctgagg agggaaactc catttccccc
    11821 acaattgtat taattgttaa gtacgctaca tatgattcat gattctcatg ttccatgcat
    11881 aagtaccggg gcttttcttc aatcaaacaa cgactgggac gtaataagtc gtaacgtttc
    11941 accaaagttt ccgacttcag ctttttcttt ggtagctgaa attcatattc ttcaaatggt
    12001 gtaccctttt cgccatagat atgttcaatc aaatacatca gatcactcgg tgtgactcgt
    12061 ctgactttaa atcttcgagc caatttactt tccattagtt tttcaagctt ggtatatcgg
    12121 cgaatttctt cattggataa ggaaacaaaa tcgcccatca aatgatgatt cactccatag
    12181 acaaattctt gaaagccgga gaagaaagat ttcttgagat tctttagatt gacctcttca
    12241 tctgtcgcaa tcagtttgaa gccaataaaa aagcgataat caatctgact atcgccaatc
    12301 atggatacta atgcctctgt ctgtaaatca attcgttgtt tcgctacttc tgttaatcgc
    12361 ccggtaattt ccttttttga tcgttcttgc gtggcacgaa tactactttc tgtggcaatt
    12421 tgtaaggcat gaatcttgcc ttcacgattt tgcgcaatca attgacgaaa gttatcgtgg
    12481 acttgatatt tctgttcagg tgacaaaaag gagtaattgt aaggtattaa ctcatagtag
    12541 gcaaaacatt ctccctcttg attaaagaca agattatttt caatatactt aatcgggtac
    12601 atagttcaca ctccttacca gagtaatggg ttcgtcccat cgtttcttct gttctttgac
    12661 tttctttcct gcgaaagtga ccttaaatcg taagtgatag ctaataaaag atcgtaaaaa
    12721 tcccaatggt ttcttaccgt caaacgtttt ttgcgacatg aaccaagtaa ctgccaccgg
    12781 tatacctaag tattttaaaa aggccccctc tatcatagat agtggaggca catgggcaaa
    12841 tacgatgaca aacagcaagg ataaaacaaa ccaagccatc tggttaaaag tcacaggaaa
    12901 aggcagttga aagtcattaa ttgcataaat gaccttttca actgcccaaa tactggtgta
    12961 gctttttatt ttcttcatca aatcattcct ttaaaaagaa ggatagttta gaaatcaact
    13021 acccttcaat aattttgtta atatggatac tcaaaaacac ctcgactcgt taccaaataa
    13081 ttgcctccaa tctccaagtc tctaccacac gcatcaaaat caatatacgt cttcaagtgt
    13141 tctggtactt ctccaaaatc accagcttct acaatcaaga aatatgctac atcactcata
    13201 tcatcgcaat ctggataata gataatatca tccacatgct ctaccatctc ctcaaaagaa
    13261 ttgaagaaag catgttgaat ctcagaagca acttcaccaa ctggcgttcc ttcaagttcc
    13321 tccgctaagc cacacaaccg attaatttcc tcaattggcg tatactcatc aatctcaaag
    13381 ggaagttcat aatcatgaat tgcatattcc tcgtattcgt catttaaccc aattcgttct
    13441 ttcacttcat caaaatcgac aggtggggta aaccacgccc ccaccaattc accttcgtta
    13501 tatttcccga ggttcgcaat ataaacacgc atctgttcca tcgtgcttcc cccttacgca
    13561 ccgacaattc ggttaaacaa actgagtaag acatccttca ccccagaagc attaaagacc
    13621 aatccgaccg caatcaaagc aatcaccaaa aagccaatca gtttggaaaa ctcccgctta
    13681 aatcctaaaa acaaaccaat gaccacaatt gccagtaaga ctaaggattg
//
LOCUS       DAYYFU010000065        13429 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000065 DAYYFU010000000
VERSION     DAYYFU010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.068"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACHA34_002268"
     CDS             complement(<1..914)
                     /locus_tag="ACHA34_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFM8792871.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACHA34_002269"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACHA34_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFM8792872.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="ACHA34_002270"
     CDS             2006..2176
                     /locus_tag="ACHA34_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792873.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEETALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="ACHA34_002271"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="ACHA34_002271"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFM8792874.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="ACHA34_002272"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="ACHA34_002272"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFM8792875.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /gene="dapA"
                     /locus_tag="ACHA34_002273"
     CDS             4440..5321
                     /gene="dapA"
                     /locus_tag="ACHA34_002273"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFM8792876.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /gene="dapB"
                     /locus_tag="ACHA34_002274"
     CDS             5318..6034
                     /gene="dapB"
                     /locus_tag="ACHA34_002274"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFM8792877.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="ACHA34_002275"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="ACHA34_002275"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFM8792878.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="ACHA34_002276"
     CDS             6751..7752
                     /locus_tag="ACHA34_002276"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFM8792879.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="ACHA34_002277"
     CDS             7749..9110
                     /locus_tag="ACHA34_002277"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFM8792880.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="ACHA34_002278"
     CDS             9070..10221
                     /locus_tag="ACHA34_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8792881.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="ACHA34_002279"
     CDS             10196..11365
                     /locus_tag="ACHA34_002279"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8792882.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="ACHA34_002280"
     CDS             11540..12919
                     /locus_tag="ACHA34_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8792883.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="ACHA34_002281"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="ACHA34_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4482 a         2340 c         2926 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DAYYFU010000066        13397 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000066 DAYYFU010000000
VERSION     DAYYFU010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.069"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..713)
                     /locus_tag="ACHA34_002282"
                     /pseudo
     CDS             complement(15..713)
                     /locus_tag="ACHA34_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            702..968
                     /locus_tag="ACHA34_002283"
     CDS             702..968
                     /locus_tag="ACHA34_002283"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8792884.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKGFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(1144..1713)
                     /locus_tag="ACHA34_002284"
     CDS             complement(1144..1713)
                     /locus_tag="ACHA34_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8792885.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1787..2110)
                     /locus_tag="ACHA34_002285"
     CDS             complement(1787..2110)
                     /locus_tag="ACHA34_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792886.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2264..3076)
                     /locus_tag="ACHA34_002286"
     CDS             complement(2264..3076)
                     /locus_tag="ACHA34_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792887.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3403..4278
                     /locus_tag="ACHA34_002287"
     CDS             3403..4278
                     /locus_tag="ACHA34_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFM8792888.1"
                     /translation="MSKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4292..4615
                     /locus_tag="ACHA34_002288"
     CDS             4292..4615
                     /locus_tag="ACHA34_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792889.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4612..5364
                     /locus_tag="ACHA34_002289"
     CDS             4612..5364
                     /locus_tag="ACHA34_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFM8792890.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5354..7525
                     /locus_tag="ACHA34_002290"
                     /pseudo
     CDS             5354..7525
                     /locus_tag="ACHA34_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7637..9808)
                     /gene="copB"
                     /locus_tag="ACHA34_002291"
     CDS             complement(7637..9808)
                     /gene="copB"
                     /locus_tag="ACHA34_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFM8792891.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9828..12014)
                     /locus_tag="ACHA34_002292"
     CDS             complement(9828..12014)
                     /locus_tag="ACHA34_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8792892.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="ACHA34_002293"
     CDS             complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="ACHA34_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFM8792893.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12236..12676)
                     /locus_tag="ACHA34_002294"
     CDS             complement(12236..12676)
                     /locus_tag="ACHA34_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFM8792894.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGEMIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12750..13289)
                     /locus_tag="ACHA34_002295"
     CDS             complement(12750..13289)
                     /locus_tag="ACHA34_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8792895.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3764 a         2807 c         2380 g         4446 t
ORIGIN      
        1 gaggggggga ttttttatcg ttgtggaata tttaaatacc aacgcttgtc atgaaaatct
       61 tgtgctccac ccttagtatt tccttctgga tcgtttgtcg cacgcatcat gacgtatact
      121 ttcttattag ggaaatcacg catattgaaa gatacatgat agccaacatt tcctaaagta
      181 ttataagctt gatttacgtc tggtctataa atcccatcag ctcttactct agctagttct
      241 tttccagtat tgtagtccat aatgaaaata tactcgtatt tataattagc aatgtgccat
      301 ccagctacat gcaagtttgc gttttcgatt tctccgaact gatcaatgtg ggcgtaattt
      361 gttccatctg tcagcgtaga atttgcagca cctgctcgag ttggatcaat tactggttta
      421 tcctccgaag tagttggatt atcatccgta aaaccatgag ccaaatcata ggctagtttt
      481 tctttactta cacccatttc agaaagataa ccgtaaggat ctgtatgatc gccccaaata
      541 ttttgtgtta cccataaatg cgatttgatt cctggttggt tataaggagt gtctaatgtt
      601 aatggaatac catatttcat tgctgaatct cttgccaatt caaagtatac tttatagttc
      661 ttttcaaaag tttctttatc atgtgtatgt tgtaactcga tttgcacagg acttttggct
      721 cacgaggttg tcctactcta catcttgctg atagatatgc tgaaaggaaa acaccaacat
      781 tatgttttgc tttttcagct agaacgagac aagcaggaat acctttagaa gatatcaaag
      841 gttttttagg tcataaagat gtttccacta cacaagtcta tgcccatatt tctcccgaag
      901 tgaaaaaaag atcaatgaat caactagaaa actatataga agagcaaata aaaaagcact
      961 caaattgagt gcttttttaa gctcactatc actaaaacta tcactaattt tatttataag
     1021 cgaacatgtt tctatcactg attttttaaa tctagcgaaa aacccttatc ttaaaggcgt
     1081 tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc ctagaagtgc gaacatggtt
     1141 ttcttatagc tcatgtagaa aaattttctt gatgatgatt ttatccatca attgagattg
     1201 actgttttgc tctcctatga tttcagctat gattgtccga ttttgcggtt ttcttcttaa
     1261 ctggtagttg attttcccag tttccatcaa ctcgctaagc atatccacga ctccgccaaa
     1321 tccggtcttt ttcgtaataa aacctagtgc taccattcgt tcatctagat tttttgctgt
     1381 aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg gccagctctt ccatcccttc
     1441 agcattggat acgacggaag ctgttttgtt aataggttct tcgtttattt cttttggttc
     1501 tttcctctcc actttcactg tttgacgaag gacatctagt tcttgcaaga gcgtttcgta
     1561 ttgttcttgg gtcagcatca cgccagctac tttttcacga ttaaatatat atacgccagt
     1621 tcccgcgtct cgcgcttgag caaatacatc catcggcgat cgtttcactt ctgtgatcga
     1681 agatgtggga acttctaatt tctttaattc caatggattt cctcctttac taatcgcttt
     1741 ttattatagc acagactgac ttgttttgat cttttttcca aaggttttat cggtttttgc
     1801 ctaaccagta attgatatcg gtaagaagtc tttcgatacc aaatgaagga tggtacagat
     1861 acgttttttg atcttcgtta tggtctagtg gaaaaaaatg atcgatgatc actagatctg
     1921 cataccgagg cggcttattt cgagtacata catgtataga gatccgtttt cgatgtctta
     1981 gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg gtgttcatag cgaaaaaaaa
     2041 agacgatttt cacttcagga gggtggacat ctaaataccc tcgttcctgg agtgttttca
     2101 gtaaaagcat cccccactct actgcggggt gtgcaagtgc ttgttggatc gctagccgaa
     2161 attgatatag tttatctttg gaattgggaa atttttctaa tgtttcctga taataacgaa
     2221 cttcttctat cagccggtca tggataccaa ttacgtaacg aagtcattct tggtaaagta
     2281 cttttgtcag ctttgatttg ctttcgctct caaattcgct attcagccaa aacagcgaat
     2341 caagcttttc agtcacaatc gtcaatttcc gatagtcttg tgtctcttct atccgtttcc
     2401 accgcttttc agtaatctgg ttacagctca agcgcaaaat tgctagataa tttttttctt
     2461 ttattggcaa aatctgctcc tctctaaata gcaattcagg aaacaattca aagaatagtg
     2521 ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt tctttgcttc aataattgga
     2581 tgaacatctc agaaaaggac tgctctccat catctgcaaa aaaatgcgga aaatctttca
     2641 aaaagcaaaa cagcagatat cttacttttc tttctgaacc aaagatctgt gcttttttat
     2701 aagaaaaaga aaggtggtac tcgccaaacc actttctcaa tcgctcttgc gtagaacgat
     2761 cgtacttctg ctcgtaaggt tcattcaata gcagattttt cagtaaacaa aacgggggtg
     2821 attggatcag aaaaacaaac aagagataag gttcgctata tccttgatca taaactaact
     2881 gaacggtttt cttatgaaga atcagctgaa tgggacagtc gctttccgcc aaacgtttca
     2941 gatgccgatc cactgtttta acatcgattt tcagttcttc agctaaatca gcttttcgcc
     3001 agatcaaatc tttcattaga ttgattagtg aaaataaatt ctgtgcattt tttgataata
     3061 gtttttgcca tctcatcgat catttctcac ctgatttcta aacatattgt ttgatcaaaa
     3121 tgggttcact cctaaaaaga taaaaaactc cctttctatt tatttaaata cgcaaatctt
     3181 cctcattatt ttttatttta ctcaatttaa tcgtattctc atttcaaaac gaatgtactt
     3241 taaaaaaaca taataagatc actttgcttg tatgatgtat tcccttccat tacaatgaaa
     3301 taaatagcga ttttatgaga aggtgatgtg aaaatggaag aaaaaataga agaatccaaa
     3361 caaaaaatcg atcagttgat ataagaatta gaaaaagcgg tcatcagcaa acgttcaact
     3421 atccaattga tgatcgtggc cctattagca ggtggtcatg tcttattcga agatattccc
     3481 ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag ctgtacaggg aaccttcagc
     3541 agagtccaat gtactcctga tctgctgcct agtgatattc ttggtttttc cgtatacaat
     3601 tcacaatcaa aagagtttga atttcgtccg ggaccgatct ttaccacgat cttgttagca
     3661 gatgagatca atcgtacgac tcctcgtact cagtcagcac ttttagaagc aatggccgag
     3721 aatcatgcaa cgatcgacaa caatacatac cctctagaca atcatttttt tgtcttagct
     3781 acgcaaaatc caatcgagta cgaaggaaca tatcctttgc cggaagcaca gctcgatcgt
     3841 tttttattcc gactacaaat cggttatcct tcttttgatg acgaactttt gctacttttg
     3901 gataaattcg aaaaagtgtt ggataactta aatgaagtat tgaacagtta tgaaatagaa
     3961 gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg aagtggcatc ttatgccttg
     4021 cagttagtgt cagcaacaag aagtcatgct gccatccagc ttggcatcag tccacgagga
     4081 agcctttcct ttattcaggc agcaaaagct tatgctttaa tccatgggag aaattacgtc
     4141 acgcccaaag atttgcagga tttagttcct tacgtgtttt cccatcgcct gattttttac
     4201 gatcgttcat tgaaggaaga cgaaaagaaa caaatcattc atactcttgt tgcacaaatt
     4261 cctattcctg tgaggtagat tgaggtagac aatggaaaaa atcaaagatt atttcattaa
     4321 ttttgtgtta tttctggtat atttcatcat tgtgcttttt gctcttgtat tcaacaattc
     4381 tttaggctgg ttcttgttgg ccttttttac tctttatgta tcatggagct tgatttcatt
     4441 aagcagcttt ctaaagaaac tttcggttac ttgtacattg cagccactcg tttatcgcaa
     4501 aaaaagtatg caggttattt tttccatagc aaaaaagaga actttttctt tacctctccc
     4561 tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa aacaagagat tttgattctt
     4621 acaaataaac cgcaagaagt ccaattgctt tggcagccaa aagaaagagg atgccatgaa
     4681 acattaactg tcatttttac agcctatgat agtctaggac ttttcagaaa aagtgcaaaa
     4741 agagagatct cttttcctgt gactgttctg cctgcttttt gtaagattca agcagaaaaa
     4801 ttacagagag ttctggaaaa aaaacagcag ctgaacgcgt accaaagagg tcttgatttt
     4861 cgggaacatc gcgcttatcg tccaggtgat tctttcaacc gaatcgattg gaaattgtct
     4921 gctcatactc aagagtggtt ataccgtgaa tacgattatc aagaagaaga atgttctcct
     4981 cttttttgct tttggggttt gcccagtcca aaatttgaac ctctgctaga cttgtatttt
     5041 tctttatggc atttgagaaa agaaaagcag ccggaacttc tgatattagg caaccgcgcc
     5101 tttcatggca aagaccccgg tcatacctat tttgcttctt tggaagctgg cgatgagaaa
     5161 gcttttttcg cacacttgaa aaaatatgca aaaaaacaaa tcgtcctctt tgttcctgag
     5221 aattctcctg aagtctcaga agctattgaa acattgtcaa aagatcgaac ggtttatctt
     5281 gtttatttcg tagaaaaaca actcatggtc caagaaaaag acaagatatg ttcattgcca
     5341 ggaggtgaat ggattgacga ttaaacggga aaaatggctg cgtggcgttt actcctttgt
     5401 gatactaagt gtaggtgctc cgatatttgt agtagctaat caattgcaaa acagctacct
     5461 tcttatccta actattggtg tcctatgcct tattgtctgc tgtattccta aaaaatgggt
     5521 acgtttgctg ctttctttgc cagtaatgat aggttgtttg tttttatatt ttccttcgaa
     5581 gacattttcc atattatgga tcttacagtt tttttcgtca gtcacagagg aattcttcca
     5641 tctatcccaa ggtaaacttt attatttgcc agaaaagaca gctttttttc tcataatgtt
     5701 gctgatctat ttatttatta cattgaccgt ggattacgac tattggtacg caccctttct
     5761 ttctttgatg gcttacttca tgatgcttac cgtttttcgg aaaaaagatt tactgtacga
     5821 aatgattgcg gtagtaactg ttttgttcgt gtatctggca gcaaggcaat ttttctccat
     5881 ccggttgttg tcgggaaaca ctcgattcca gtcattgact acagttctgc ttatttgttt
     5941 cttaactttt agcgctatct tgcctttata tcttccgaaa gtacaccaat ctctcgagga
     6001 aacgagtgaa ccgatccgag aatatcttaa tgatgaaggg ctttatgaca cactccatga
     6061 gtatcaatta accggaagtg caagaacagg cttcagtgag aatgatgaac agcttggtgg
     6121 acctttatat gataatggcg aagttgtttt tacagcgaac caaaaaggaa atggctattg
     6181 gaagatcgaa aataaaaatc attacaccgg ttctggatgg atagaagatc aggacgcacc
     6241 catccaacca atccaagaat tccctgttga catcacgagc taccaaaccg ttatagaaga
     6301 caaagaaaac attacattga ccctaaaaaa acaagttttc ttaccttatc cttatggaaa
     6361 tactaccttg actgattttt cattagctag tggacaaacg ctttactatt tgcctgaaag
     6421 ccagcgattc attcctgtcc cgaatgaatc gacacgttct gcaactttac aaacagaaag
     6481 gttagttttt acgagagaag aattaaaaag cagtcagtta aaagctgatg atttagaaaa
     6541 tcagcagaca aatcttcaat tgccaaaaag tttgcctaaa cgtattgagg acttggcggt
     6601 cgaattgacc aaaaatgaag caactttaat tcgtaaagtc gaagctgtct aacagtattt
     6661 aaaaacaagc ggaaatttcc gctattccaa aacggatacc gggcatactc caaaagaaca
     6721 cgattatgtg gattatttcc tctttgattc aaaagtcggc tattgtgaca acttttcaac
     6781 ttctatggtc gtccttcttc gaacacttgg tatcccagct cgctggacaa aaggttttac
     6841 aaatgggctc agaacaggca cagataatca agggaatgag gaatacagca ttctcaatag
     6901 tcatgctcat tcttggccag aggtatactt tcctggattt ggctggcttc catttgaacc
     6961 aaccccttca tttgccaatc caatgggttc acaaccagtt gctgaaacaa ccgaaaccgt
     7021 ccagcctgct atcgaacaac caaatgaaag cgaaaatatg gcagaaacta tttccagtga
     7081 gagatctgag gtcattccat cagaagaaac aacactcaca ccagcttata aaatcgtctt
     7141 attcagcctt ggagtgtgtt tatccgtctt gttgttcttt ctattgttat tcagaaaatc
     7201 tctgcgctgg acaagttatc ttatctatct tcattttttt gcttctacta atacgattgc
     7261 atatcaaaaa cttcttaacc ctttggaaac attggtaccg cgcaaacccg cagaatcttt
     7321 gcaggaatat agcctgcgaa tagaacaaga attcaagcta gatctgcgtt tcattcgatt
     7381 aactactcaa tatgaagcag aaatatatgg acgacaagca gatccggtaa aattcgataa
     7441 aaaaacctat ttagaaataa cgcatataat aagaaaaatg gtatacaaaa aaattctgac
     7501 aaaagggagc cgcttcaaaa aataagataa aatttctgga gaatctactt ctggaatgcc
     7561 gagaaccagc tgattgagta tcgattactc gaaaaaaagg ttgtgacacg ttttttgtca
     7621 caaccttttt tcattattat ctgattttca atgtcaaagc atttagagct acaactaccg
     7681 tacttaacga catcaagaca gcaccaactg ctggacttaa tatgatacca attggcgcaa
     7741 gaattccggc tgctagcgga attgcaagaa tattgtaacc tgccccccac cagaggttct
     7801 ggatcatttt ctttcgcgtt tgcttggcta gatcaagaaa atgcaaaata tcttttggat
     7861 cgctgtttgt caatacgaca tccgcagaat cgattgcgat atctgtccct gcaccgattg
     7921 ccatcccgat tgaagcacgg gctaaacttg gcgcatcatt gatcccatca cccaccatga
     7981 ttactttttt cccttggtca aggtatttct ggacaacagc ttctttatca tctggtaata
     8041 aaccgccata atattcttcg atccctaaat aatctgctac tgcttgggca gcgtttttat
     8101 tgtcaccagt aagcatgaca ggagtgattt ttctttcttt caatgttcgg ataaattctt
     8161 ttgcttctgt ttttactacg tcacctaaag caacgattgc agctagatga ttttctaaaa
     8221 tcaggaagct gatggtattt ccttgttctt ggtaaggttt caataatgct tgttctacgt
     8281 ttaatccgag acgctcaact tctttttcat taactatttt cacttgttgt tttttgactg
     8341 ttgcctctag cccgactcct gaaagatttt ttagattttc agcttgataa ggcttgattt
     8401 catgttcttt caaataattc ataatgccaa cagccaatgg atgattggca ttcgcttcca
     8461 aggctcctag atattgaagg atttcatttt tattgaacgc atcatcaagt acttcgacac
     8521 ccgttacagt aaattttcct tctgttagtg ttcctgtttt gtccagcatg atcacatcta
     8581 gatcattggc ttgctccagt gcattgcggt ttttcaaaag taatccgttt ttagctgcta
     8641 ttgatgtgga acgagcaaca actaatggga tagctaaacc tagagcatga ggacaggcga
     8701 tgataaagac tgttaccata cgttctaatg cattaggaag atctgtcaag aacaaccagg
     8761 cgatgaatgc caggataccc acaattaatg cgacataaaa cagccatttt gccactttgt
     8821 cagataatga ctctagcttg gatttttctc cttgtgcttt tcgcaccatt tccataacct
     8881 tcgctagata accattttca ccagttccag ttacttcgat ttcgatcgtt ccatccccat
     8941 tgatagaacc gccaatcact ttgtcattgg cttgtttttt gattcctctt gattcgccag
     9001 tcacagctga ttcgtcaaca attgtttcac ctttcaagat tacaccatct gttggcattt
     9061 tatcaccaga ccggacgatc aaacgatcac cttcgtttac ttctttcaat gaaactgtct
     9121 cttcttttcc atctttcgtc aagcgtttca ctgattctgg cagtaattct gctagttttt
     9181 gtaaagcatt gctggcattc gacacagcat tcatttcaat ccagtgacct agtagcataa
     9241 taacgatcaa agtagccaat tcccagaaga aatccatgac gtgtgtatgt ggattgagca
     9301 gatttgcgat aaacgaatag acactataaa tatatgcaac tgtaatcccc atcgcgatca
     9361 gtgtcatcat agcaggactt ttttgtttca attccatctt agctccactt aaaaatggct
     9421 gaccgccata gataaatagt atagttgcta agatcaatac gagccaatca gaaccaggaa
     9481 acgttacttg aaatggaaat tccattccca tcattggtga aaaaagaatg attgggatag
     9541 ctaggataag cgatagccaa aatttttgtt tgaaattccc catgtgcatg gaatgatcca
     9601 tgccgccgtg ttcgtgatcc atgtgcatcg aatggtccat ttgtttgctg tttgctatat
     9661 ctccatggtt atgtcccatg tgcatggaat gatccatctg actatggtca tgtttcatct
     9721 ccatctgttt ttcatgagat tctgtacttt ctttgtttac tacttctttt ttatgctcat
     9781 gttttttctc catatcgttc ccattcattt ttactccatc ccttctctta ctttttcatg
     9841 tgtcgattta agcacaggga gttcaacaat acactgacgg aactaaacgc catcgctcct
     9901 cctgcaatga ttggattcaa aaagcctagt gcggcaaaag gaataccaat cgtattatag
     9961 acaaaagccc agaaaagatt ttgtttgatt tttctcaatg tcaacctaga caactcgatt
    10021 gtttgtgcaa tggatgccaa actgctattc atcagcgtca catcggctgt ttccatcgca
    10081 atgtccgttc cacttcccat tgccataccg acatcagcta atgctaaggc cggtgcatca
    10141 ttgattccat cgcctgccat accgactttt ttgccatctt tctgaagttt ttctacgtag
    10201 gctgctttat cctctggaag tacttcagca aagatatgct ttggatcaat accaacttgt
    10261 ttaccaatcg tttcggctgc cagtttgttg tctcctgtca gcataaaaac atctaatcct
    10321 ttattctgga gctgcttgat tgcttgtttg acttctagtt tgatttgatc agccacggca
    10381 atcaacccga tcacttgctt ttcatcagct aaaaacatga ctgtttttcc ctgctgttct
    10441 aattctaacg cgtgttcttg gtattcatca aatgataggt tcaattcaat taatcgttta
    10501 cgtgtacccg caaaatagcg tactccgtta atcgtaccgc tgattccagc acctgggtgg
    10561 gctgtaaaat caatcatatc gtatgttgct gcttcttgca gttttccgta ttccacgatt
    10621 gctttaccta gtggatgttc agaagcttgt tctaaagtat aaaaaataga taggacttct
    10681 ttagaaccga ctagatctgt aactttgggc tttccttctg taatcgttcc agttttatcc
    10741 aaaacaatgc tgtctaagtg agcagctgct tctaatgctt ctccgccttt aattaaaatt
    10801 ccatttcgtg caccaacacc cgttcctacc ataatagctg ttggagtcgc taaacctaat
    10861 gcacatggac acgcaatgac taatacagag acactatgga tcaatgcgag ttgccaatca
    10921 ccagtgatca aacctgtagc tatgagcgtg ataaacgcga tgactaatac gatcggaaca
    10981 aaaatccctg agatccgatc tgcgattttt tggattgggg ctttgctccc ttgagcatct
    11041 tccaccattt gaatgatttg cgctaatact gtctgcttgc cgacttgtgt aacttccatg
    11101 tgaagtagac cattcgtatt gattgtcccg ccaaagagcg tgtcatccgg attcttttct
    11161 acaggaagat tttcaccagt aagcatactt tcatctatcg cactgcttcc tgaaataatt
    11221 cgaccatctg ctggaatctg ctcaccagga cggatgacta gttgatcacc aacgactact
    11281 tcttcaatcg ctaacgtttc ttctttcccg tttcttatca cttgggcggt ttttgtttga
    11341 agagacatca attgtttgat tgcattacct gttttcgttt ttgctgtatg ttccaaatat
    11401 ttgccgagca aaatcagcgt gatgatcata ctgctgcttt caaaataaag atcttggggg
    11461 tgtccacgga aaaatccgtt gtaaatgctc aatgcaaagg cagccgatgt tccaattgct
    11521 accaatacat ccatatttgg tgctttcgtc ttgattgcat ggtacgcacc tttataaaaa
    11581 cgagcaccga cataaaattg gacaggagca gacagcacta actggacgat tggaaaatga
    11641 aaaaagtgaa caatcgccgc atggcttcct aacatcatag caatcattga caacatcaac
    11701 ggcaaagtca aaaccgtact caatataaga tcgaatagca tttttttcaa atacgcttgc
    11761 ttttcttctg caattttttg tttgtgggct tcatcgtaca aaattgcacc atagccgatg
    11821 ttttctacgc tttggattaa tttttcagtg gtcgtccctt catatttgac acttgctttt
    11881 tcagtggcca agttgactgt ggcattgacc acaccaggtt gtccgttcag ctctttttcg
    11941 atcctagcag aacaatttgc acaagtcata cctgtaatga cgaacgtatc cattttggtt
    12001 tctgctgttg ccatcaaatc acctctgctt tataacccaa tccattgatt gcttgacaaa
    12061 tcttttcgga ttgaactttt gtttcatcaa acttgaccgt accgtttgct tttttcaaat
    12121 ttactttcac tttttgcacg ccgtcaagtg cgcttactgt ttcttctact cttgctgcac
    12181 aatgattgca gctcatacct tcgattgaaa attgttgttt cattttttct tcctcctatt
    12241 gatgttgttt acattcacat tgacctggga tacagttaca ttcgatcgta tctactggtt
    12301 gcttttgttc cagttgtttc aaaatctcat tgatatcttc ttgagtcaaa gtggcttctt
    12361 ctactagatc cgcaatcatc tctccaatcc tttttgcaca gatatgggaa aaaagatttt
    12421 ccgtcgcact gcgaactgtt tccatctccg agacagctgg ataataaatg aatttttttc
    12481 cttcttgttc tgtccagatc acttcttttt ttaccaaacg acccagcaaa gttttgatcg
    12541 ttgctggttt ccaatccatc gaatcagcta agatctgcgt gatttcttgg gctgtcgtct
    12601 gtccaagtgt ccaaataaca cgcatgatct cccactctga atcactgatc tttactggcg
    12661 ttttgacttt ttccatttat ttcacacctt tcgtttacaa atgtaatcat tatcttatga
    12721 atacagatta cacttgtaaa caaaaactgt caattatttt tgctctctat tttcaattta
    12781 gttaaaaatt cttgtttctc tcgtcttgtt ttaaggatga tcagtttatt tcttgcatta
    12841 gctacttgga tcagctgctg tattttcggt tcattgtttt cttcaaaatg ccagataaaa
    12901 gataaaaatt ctaagtattc cctgtttagt ttctccgaaa atgattcagg catatctggc
    12961 cgagattttt tgtttatctt tctttttaga gaacgccaaa ctactcggta gatggcgaga
    13021 taacgcggca ctttaagcca aatgatctta tctgcttctt taatcctttc ctctaatgta
    13081 gaagtataat ttccttcaac gatccatgac ggattagata ccataaacaa ttgctgttct
    13141 tgcaaaaacc acttttttgc ctgaatactg tagtctgttg tatgccataa aaggtctagc
    13201 ggcagtagcg gaaagtcggt gatttctttg atcttccttg cgagggtcga ttttcccgac
    13261 ccactttgtc caatcacgat gatcttcatt cttctccctc acttctcttt ggaatttggt
    13321 tcgatcatac cacttataac gccgcaaaaa aacaagggac tgtgacaaaa gtcgtgtcaa
    13381 agtcccttgt ttcgaat
//
LOCUS       DAYYFU010000067        13259 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000067 DAYYFU010000000
VERSION     DAYYFU010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.070"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            148..1887
                     /gene="yfcC"
                     /locus_tag="ACHA34_002296"
     CDS             148..1887
                     /gene="yfcC"
                     /locus_tag="ACHA34_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFM8792896.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2044..3228
                     /gene="iadA"
                     /locus_tag="ACHA34_002297"
     CDS             2044..3228
                     /gene="iadA"
                     /locus_tag="ACHA34_002297"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFM8792897.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3339..3542
                     /locus_tag="ACHA34_002298"
     CDS             3339..3542
                     /locus_tag="ACHA34_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8792898.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3643..4305)
                     /locus_tag="ACHA34_002299"
     CDS             complement(3643..4305)
                     /locus_tag="ACHA34_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8792899.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4318..5820)
                     /locus_tag="ACHA34_002300"
     CDS             complement(4318..5820)
                     /locus_tag="ACHA34_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8792900.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6368..8047
                     /locus_tag="ACHA34_002301"
     CDS             6368..8047
                     /locus_tag="ACHA34_002301"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8792901.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8174..8653
                     /locus_tag="ACHA34_002302"
     CDS             8174..8653
                     /locus_tag="ACHA34_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFM8792902.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8872..9552
                     /locus_tag="ACHA34_002303"
     CDS             8872..9552
                     /locus_tag="ACHA34_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFM8792903.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9560..10891
                     /locus_tag="ACHA34_002304"
     CDS             9560..10891
                     /locus_tag="ACHA34_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFM8792904.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10906..11868
                     /locus_tag="ACHA34_002305"
     CDS             10906..11868
                     /locus_tag="ACHA34_002305"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFM8792905.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11979..12317
                     /locus_tag="ACHA34_002306"
     CDS             11979..12317
                     /locus_tag="ACHA34_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFM8792906.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12396..13103
                     /locus_tag="ACHA34_002307"
     CDS             12396..13103
                     /locus_tag="ACHA34_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFM8792907.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4221 a         2338 c         2907 g         3793 t
ORIGIN      
        1 tcatacatag gagaaaaaaa acaaaataga aaaaagtgat tttctaataa attattcatg
       61 tggaaatgtc atttttactg tataattcat ttgattatcc attttttatt catcaaaatt
      121 actgaaaaaa gaaaggaaga aataaatttg aagaaaacaa attcttcagg aaaaccgaaa
      181 cgcgacttca ctaaattatc gacaccccat acgtatgtca ttatttttgg tgtagtcatc
      241 tttgcatgga tactgacttt tgtcgttcca gcaggaaagt tcagcacgca agatattgaa
      301 tacaaagatg caaatggcga aacaagtact cgtactgttt tacgtcaaga ttccttccgt
      361 tatgcttacg agctggataa gtcgtatgtg tttgatcaat tagaagaact gcaagatcat
      421 ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat tggaaaaagt cattgctgac
      481 ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct ccttgacaga tgatgtgctt
      541 tacgatcagt acggcgaaaa tatctatgat acttctaaaa aattgcataa aacagccaaa
      601 atatggggaa cagatgattt tggcggcttt ggtttcttaa attttgtttt tgaaggacta
      661 gtatctggag ataagtatgg atctgctgta gggatcgctg cattgatcct tgttgttgga
      721 ggtgcttttg gtattattat gcgtaccgga gcgatcgacg caggtatcta tgcgtttatc
      781 agtaaaacta agggactgga gcgtttagct ttaccgctat tattctttgc tttttcattc
      841 ggtggcgcta cattcgggat ggcagaagag gtaattccat tctcaatggt gatggttcca
      901 tttgtcattg cgttaggata tgactcgatc gtagcagtaa ccgtgactta tgtcgcatct
      961 caagtcggaa atgcgacctc ttggatgagc ccgtttagcg tagcggtggc tcaagggatt
     1021 gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga ttatgtgggt agttgttact
     1081 gcattagccg ctggttatat gatgatatat gcagaaaaaa tccgtaaaaa accagaaagt
     1141 tcgttgactt acaaatcaga tgattatttc cgttctcata tcaaaaagac atctgatgaa
     1201 aataaaaaat tcatgcttgg gcataaattg atcttattag agatgttagt tgtccttgtc
     1261 tggatcgttt ggggcgtgac tcaaaaaggc tattacattc cagaaatcgc ttctcaattc
     1321 tttgtgatgg gattagttgc tggaatcatt gctgttcttt tcaaattaga tggaatgaaa
     1381 gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg atttagctgg tacagcaatc
     1441 gtagtaggta tggccaaagg gattcttctt gtcttgggag gatctgatgc atctgttcct
     1501 tcagctttga atacgatttt gcatgggatc gggacggcgt tgaccggtgt tccagctgtc
     1561 attggtgctt gggcgatgta catcttccaa agtttgttca acttggttgt tacatcaaat
     1621 tcaggacaag cagctttgac tatgccaatc atggctccac tggcagattt agtaggtgta
     1681 tccagacaag tagcagttct tgcttatcag cttggttcag gttttatgga cgcttttaca
     1741 cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac ggatcgaatg ggcaaaatgg
     1801 gcaagattcc aaatcaagat gcaaggattc ctgtttgttt taggaactat tttcatcatg
     1861 attgccattg caataggtct gcagtagtac tggagacctg taaataaaaa agtaatccaa
     1921 acagtagata aggactccaa gaaccgtcat tttacggtaa aatcaaagga gtaaagatcc
     1981 tatcgtttgg attatttttt aagatcctat catcagttat acttaaagaa agaggaaaaa
     2041 tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag attacttagg aaaaatggat
     2101 gtactgattg ccggtgggaa gattttggct gtagaagaac agctagaagg cggctacgaa
     2161 ggagtggaag tagaagaact ttcaggtgag ggaaaagtac taaccccggg tttcattgac
     2221 tgtcatttcc atattttagg tggaggagga gaaggcggtt atcaaaatcg aacgccagaa
     2281 gtcacattga gccaattgac tacagctggt gtaacgacag ttgtcggttg tttgggaaca
     2341 gacggagaag gtagagatat gactgcgttg atcagcaaag cgaaaggtct ggaagcagaa
     2401 ggaatctcca cttatgtata cgaaggttcg taccgattgc cggtaaaaac agtaacagat
     2461 tctatcatca aagacttttt gacaattgat aaaattatcg gaatcggaga aatcgcagta
     2521 tccgatcacc gttcttctca gccaagtttt gaagagttcg ctcgtgcagt agcagatgct
     2581 cgtgtaggag gcatgttatc cggaaaagct gggatcatca atgtccactt aggcggagga
     2641 aaaagaaagc tagagctatt gacacgaatc gtagaagaaa cagaaattcc gatcacacaa
     2701 tttttgccta ctcatgcaaa tcgcacacca gaattatttg aagcttgcgt ggcatttgcc
     2761 aaacgtggtg gcacgattga cttcactgct agcgaagatc ctgatttttg ggagaagaca
     2821 gatggtgaag ttcgcttcag caaagcactg aaacgattga tcgaggaaga tgtttcttta
     2881 gataatttca cgatgacctc agatggacaa ggaagcttgc cttattttga tgaaaacaat
     2941 catttcctag ggttaggagt aggaagtgcc aaggcattgc tagtagggat caaagaagct
     3001 gtgcaaaaag aaagtatccc tttggaaatc gccttacgag ctataaccag taatccagca
     3061 cgtatattga aattggataa aaaaggaaaa atcgaaattg gtgctgatgc agatctgtgt
     3121 atcttggata aagaaacgct tgatattgat acagttatcg ctaaaggaga aataatggtc
     3181 caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa gtttttaatc aacagctcta
     3241 actatctttt ttagaaaacg agcgttctgt ttgtctgtat ctgttgttga tgttaccctg
     3301 aaggtagatc aataaacaaa ctaagaggag gaagcacaat ggcagattta aaaggacgtt
     3361 ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga agcatacggc aaagcaacag
     3421 acgacaagag caaagaaaca gaagggaaag cacaatcact aggtgctgac atcaaagaaa
     3481 aagctcgaaa tttaggcgac gaagtcaaag acaagttttc tcgaaaagac gaaaaaaaat
     3541 aaaaaccact tattactaat tgaatcaagc aaataaaaaa tccgaaccat atctgcgaga
     3601 cagatatggt tcggattttt tatttgatca ttatcctgtt ttttaaactt cgattttttg
     3661 aaatttttta ttttttagtt caaagacttc atcacagctt tttgctactt caatctcatg
     3721 agtcacgatg ataatacagc gattttgttc atgggcgatt tcttggaaaa gtttgacgat
     3781 atcttgtgaa gttgtttcat ctagatttcc agtaggctca tccgctacga ttagttgatg
     3841 ttcacagcag atagcacgaa cgatagcgac tcgctgctgc tgacctcccg aaagtttggt
     3901 cacctgtttt tttgctaata tttcatcaat tcctacacga gccagtcgat ctaaagcaac
     3961 ttgtcgataa ttctcttgct tggtcttagt gatttccatt gctgctatga cgttgtctag
     4021 agccgataaa taaggaagca agttgtaagc ttggaaaata atcgagacat catttttacg
     4081 ataacttctt aacccgattt tgttcagtga acgatcttga tagtatacct ttccttcttt
     4141 tggtgcatct agtccagaaa taagcgaaag aaaagtcgtt tttccagaac cacttgctcc
     4201 taaaatagcg tacatttttc cagcttcgaa attcagttct acatctttat aaagcgcatc
     4261 ttgcggatct tgataccaaa atcccaattg ttctgtccgt aacatcaaat tcctccttta
     4321 gtttaatagg atttttcttg ggtttaatct taatatccct atagaagcta aaaagacaga
     4381 aaacagactg attccaaatg ctactccggc taatagtccc aattgtgcag gtttgacatt
     4441 gatttccaat tcgctgactt gatcagaaac agcaaatgga ttattcgtgg ttccttgttg
     4501 accatcgcgt tgaccgcctt gttcttgtct gttacctggc atttgtccac cgccaggagc
     4561 ttgctggttt tgtgtcgtgg tttctgtctg ttggtcaagt agctgctgtc ccacggcatt
     4621 cgcgacgacg tttccgctaa gcgaagcaac tcctaatgca agaatcatac aaatagccac
     4681 ttcggtaaac aattggagaa tgattttact tctggattct cctaaagata gcaagacgcc
     4741 taactcatgc cgacgctccc gaatggtaat cataatgatc agagtaagta tgataatacc
     4801 ggctacagcc actagtaaaa caacattttt agaaaaactt gccacgttgt ttaaaggttc
     4861 caacattgac tgatacatgg aatcatttga ttgtaagcta aatgtatctg tgtcgatcag
     4921 cttctcagct tctttgagaa actcatccat tttatctgga tcagatagag tgtaaatagc
     4981 agaatctatc gtgttatcgt cagatgtccc atttagttca tttgcaaaag tgtaagaagt
     5041 gtaaagtgta ttcgacggat tcatgaagtt gaatttcata cccatactgc cgcttgtttc
     5101 gctggattca taaattcctt tgatcgtcat ttcataagtg gtatcttcat cttcagtact
     5161 cgtgattgtg aaagtatctc cgactgccag gtcatttgtt tctgctagag tagaatcaat
     5221 gactacattg tttgtgcctt tgtcgctgtc atctattcct tcaccatccg tgatttttgc
     5281 tgttccatca gaaaaattac tattttgtgc agttgctgac acaccactga tttgaaagtc
     5341 tgcttgagtc atttgcggat tcccgcccat tcctccagca ggttcaccgc ctgcatcagt
     5401 agtttctgaa ctgtcttcac ttgaatcaga actagtgatg gcagttattc catcttttgc
     5461 catagctgag gcggaacttt caaaggaata actctttaca ttatctaatt cagctatttt
     5521 ttccgcgtcc gttaaggaaa ctggtgtaag agaaaaactg cctggatctg gacgttcttc
     5581 agtggtagaa ccgcttgaag aagtttgttc ctgtttttta aatgaagctt ctcgattggc
     5641 acttaaagtc actgttgctc caacttcttt ttgtgcttga ttcgctgctt cttctgcagc
     5701 gcttcgaatc gtgaggccag ctagcacaaa aataagaatg gctgtgaaca cggcaaacaa
     5761 taacaatgaa cgtccccatt ttacttttgt acttttccat gcacgtttca taaaattcat
     5821 attttgcatc cctcctagta gatgcatttg ataatagaca acctatctta aagctagctt
     5881 aaatcttatg ctgtttgcac accatcaaca tgaggttctg tatatataaa aagtcactat
     5941 agttattgca aaaaatgtgg taaaatgaaa aaagtatgtt tcaaaccgac gaagaagaaa
     6001 tgaaagagga atagcaaaag aaatcctaga tgctacagag ggatttttac gtgcatagtt
     6061 gttctaacaa ttgctgatac tagcgattta gtttatttgt tgtataataa tggggaacag
     6121 ctgaatactt atataagtat tctggctata gcgtttcgca aaagatccat tttgttaatg
     6181 aagtatatcc aaaataaaat gcccatccaa ctgtcagaac gcggatgtgc tggtaatttt
     6241 cctgctgatt ctccataagt tgagaaatag tgattttgca tatgcttcat tggaatggct
     6301 catatattta gataaagaag aaaatcgaca tctttaaact tggatgtcat aagaagaggt
     6361 gaagtttgtg agtaccatta aaattatccc attaggcggc gttcgtgaaa atggaaaaaa
     6421 catgtatgtc gcagaagtgg gcgaggatat ttttatttta gattgtgggc taaaatatcc
     6481 tgaaaatgag ttgttgggga tcgacgtcgt aatccctgat tttacgtatt tagaagaaaa
     6541 cattgatcgt gtagctggtg tctttttaac acatggacat gcagatgcga tcggggcact
     6601 tccttacttg ttgtcaaaaa tttctgtacc agtattcggg acagagttga cgatcgagtt
     6661 agcaaaattg aatgtcagtc gtaatgaagc agccaaagga tttaaggatt tccatgtggt
     6721 agatgagcat acagaaatag actttggtga aacagtagtg agctttttcc gtaccactca
     6781 tacgatacca gattcagtgg ggatcaatct gaaaacaaaa gaaggtagta tcgtttatac
     6841 gggagatttt aaatttgacc aaacggctat cccaatgtat caaacagatt atgcacgatt
     6901 ggcggaaatc ggcaaagaag gagtcttagc cttactaagt gaatcggcaa atgcagaaaa
     6961 tcctcagcct gtagcttctg aagcccaaat agcagatgaa gtatttgata cgatcaaata
     7021 ttgggaaggg cgtatcatcg tcgcttgtgt agcaagtaat ttgcaacgag tgcaacaagt
     7081 attggatgca gcagaaaaag cacaacgaaa agttgtcttg actggtcaag atttcgaacg
     7141 aattattcgc acagcgatgc gtttagaaaa acttcaatta ccaagtgaag atctgcttgt
     7201 tacgttgaaa gacatgaaaa actatgatcc agaagaactg atcattttag aaactggccg
     7261 aatgggtgaa cccatcaagt ctctgcaaaa aatggcaaac ggtacacacc gtaatgtcaa
     7321 gattgaagaa ggagatttgg tttatatcac tactacgcca agcatcgcga tggaaaccat
     7381 cgtggcgaaa acggaagata tcatttatcg agctggtgga acagtgaagt tgatctctga
     7441 gaatatgcgc gtttctggcc atgccaatcc taatgacttg caattaatga tcaatttgat
     7501 gaaaccaaca tattttgtgc cagttcaagg agagtatcgt gaattagctg ctcatgcaga
     7561 tttggctcat gcagtaggga tgccatacaa aaatatctat attactggac gcggagacgt
     7621 gctggaatat aagaacaagc gtatgagtgt tgcaggaact acaagtgcag aaaatgtaat
     7681 gattgatgga ttaggcatag gtgatatcgg aaacattgtt ttacgtgatc gaaaaatctt
     7741 gtcagaagac gggattttcg tagcggttgt cactatcagt cggcgagaaa agaaaatcat
     7801 ttcaaaacct caaattactt cacggggatt cgtttacgta aaagctagcc gtgatctgat
     7861 gaaagaaagc agcaatatga ttgaaacaat cgttgagaaa catttagaaa gcaatgattt
     7921 tgagtggagc aaattaaagc aagacatccg agatcaatta ggccgttact tatttgaaca
     7981 aacaaaacgc cgcccagtga tcttgccagt cattatggaa gctactcaac gtcgaaataa
     8041 aaaataaaca aaaagagtaa gatgaacctc tctatagagt gctcgtcttg ctcttttttt
     8101 gcgaaaaaca aataattttt gaatacagca tccctcgctt tataataaac gatagaataa
     8161 agggagtgaa tcaatgatga gtcaaacaaa agtcgaacgt tatttagaag aacaaaagat
     8221 cagctatcaa ccacttgatt ttagtcatgt ggtagaagaa tctttcacag aagaactaca
     8281 aaaaagaggg attgatccag ctttgatatg taaaactctc gtagtgaaag gaaacaaaac
     8341 tggggtgatc atcgcattga ttcctttgaa cgatcactta gattacaaaa aaacgagaca
     8401 agtgacgaaa gatcgtaaag ttggttttcc tggaatggat tttgtattaa tgcatacagg
     8461 atatccccac ggagcaaaca cccctattgg aataaaactc gcacatcctg attattgttt
     8521 tttagcagac agttctttaa aagaaaaaga agaagtaatt gtgtcaagcg gtgaaatcgg
     8581 gagaagcctt cggataaaag tagccgattt agaaaagatc gtccatccta tttacgctga
     8641 tttaattagt taagggacga gaagagaagc taagcagagc agagacctca aacttgagat
     8701 tatatctgct tggtacttgg tgcatcctgt attatctgtt acaattgctt tgtacatact
     8761 gtactatcaa tccaatgaaa atatatttag cagctatatt ttttcattga tgtgcaagtg
     8821 atgaaatcgt ttttaataaa aaagatacag agaaaacgcg aggtgtaaaa tatgaaaatt
     8881 acgtatcatg gacattcctg tttaacaatt gaaatggaaa atggccaacg cgtgatgatt
     8941 gacccgttta ttacaggaaa tccattgaca gacttaactg cagaagaagt aaagacagat
     9001 tggttgctga ttactcatgg tcatagtgac cacataggag atatgattcc tattgcaaaa
     9061 gaaaatcaag ccacaattat cagtattgtt gagattgctg attatgcgaa cagcagagga
     9121 gtagattcat tcggtatgaa tatcggtgga aaacatgcgt ttccattcgg cgtggtaaaa
     9181 ttcgtacatg cacagcatag ttcaagctat gaagtagacg gtatcgttca gtatatgggc
     9241 gagcctagcg gaattattat tcaagcagaa ggaaaaacga tttatcatgc aggggatact
     9301 gcttatttca gtgatctagg attgcttgca gaagaattcg atattgatgt cgctttcttg
     9361 ccgattggcg ataactatac gatggggcca gaagatgcag caaaggctgc gcaaacaatt
     9421 cgtgccaaga aagtagttcc aatccattac aatactttcc ctgtcattga acaagatccc
     9481 gaatcttttg tagagttact ggaagatagt gaaggaaaaa taatgagcgt ctcggaaacg
     9541 ctggaagtgt aggcagaaaa tggcgacaaa acatgatttg attttagaat atattgaaag
     9601 tttacctgtt ggcaatcgaa tttctgtaag aagcatcgca aaagaattga atgtcagtga
     9661 aggaacagcg taccgtgcaa tcaaagatgc agaaaatgtc ggactagttt caacaattca
     9721 acgagtaggg accatccgta tcgaacgcaa attaaaaaaa catattgaac gtttgacatt
     9781 tggcgaggtc gtcaggatca ttgaagggga cgttttaggc ggggcggcag gattagacaa
     9841 ggtcttgaat aaattcgtca tcggcgcgat gactaaacat gccatggaac gctatattac
     9901 gccaggatca ctaatgatcg tcggcaaccg ggcggaagtc caaaaattag cattggaaga
     9961 tggcgccgca gtattgatca caggaggatt caatacgact gctgagattg caaagctagc
    10021 tgatgatctt gaaatgccga tcttacgcac ctcttatgat acatttacag tcgcaaccat
    10081 gatcaaccga gcgttaagcg accaattgat caaaaaagat atcatgcttg tcagtgatat
    10141 ctatatgcca gctgaaaaga ctcattattt gcagcagaca gatacgattt ctgattatca
    10201 gcgtttgtca gaagaaacgc agcattcgcg ttttccagtc gtcaaccgcc atcatcgttt
    10261 gatggggatt gttacggcaa aagatgtttt agggaaatcg ccaaaccaga tcatcgatcg
    10321 ggtgatgacg aaagaaccaa tcagtgtaaa gaaaacaatg agtattgcct ctgttagcca
    10381 tcaaatgatc tgggatgggc tagaagtcat gccggtcgtt tccgatgact tgacattgga
    10441 aggcttgatt acccggcaag atgtaatgaa agcgatgcag cttgtccagc gacaaccgca
    10501 gattgcagat acgatttcgg atcagatatc tggaaatatc catacgattg atactgaccg
    10561 tgaaggaaat cgtttggaaa accctaagtt caaattcgta gtagcaccac aaatggtcaa
    10621 tggcgttgga accatttctt ttggcgtact tagcgaaatc atttctgatg tcgcacaaaa
    10681 aacaatggtg atgaaccaaa aacgaaatat cttgattgaa caagtgaacc tacattattt
    10741 gcggctgatc caattggaaa gcgaattaga cattcgtcca cgtgtattgg aaatcggcag
    10801 acgttccgct aaactggata tcgaagtgtt tattgaaaac accatcgttt ccaaagcaat
    10861 cgttgtttgt caagtaatgg aacgttctta gaaagcagga gtgaaatgac agtacagcaa
    10921 gaaatagtag atacaataaa aaaatacgat aaaatcatta tccacaggca tttgcgccct
    10981 gatcctgatg cattaggatc acaagtagga ttagccgaac tattacggga aagtttccca
    11041 gaaaaagaaa tacgtcaagt aggtgaaacg attgaagggc ttcgcttttt agcagaaatg
    11101 caagaagttg aagatgtctt ctaccaagga gcgctagtca tcgtgactga tacagctaat
    11161 tcacccagaa tcagtgatca aagatatcag ttggcagaaa aactgatcaa aatcgaccat
    11221 catcccaatg atgaccctta tggagatctt gtgtgggtaa atacaaaagc aagtagctgt
    11281 agtgagatga ttgcagaatt tgctttgatg tttcctgaat ctttaaagat gaacgttaag
    11341 gctgcacggt tactttatgc aggaatcgtc ggagatacag ggcgcttttt gtatccctcc
    11401 acgacgtccc gtacattaga gattgcttcg atattgagaa attatccatt tgatgcaagt
    11461 gctttgaaca gagagattga acaaatgccg atgaaagtcg ctaaactttc cggttatctt
    11521 tatcaaaata tccaggtgga tgaaaatggt gcaggaagaa taatccttcc gcaagaaatt
    11581 cttgataaat atggaatcga tgattcagag acagcttcga tcgtttcact tcctggagtg
    11641 attgacgaag tgctggcttg ggggattttt gttcagcagc cttcagggta ttaccgcgta
    11701 cgtttacgtt ctaaggggcc gatcatcaat gaattggcta aacgccatca tggaggaggc
    11761 catccattag caagtggtgc gaatgcaaaa gatttagcag aagtagagga aatctataaa
    11821 gagatccaag atatatgcca atcgtataag agcagaaaaa cagaatgata cttcgttcga
    11881 aaacgtgcct tttgtagtaa aaatcacaaa tgtatctttt caagatcttc tttttccgtt
    11941 acaatagtgc tcactaaaga ttttaaagga gaaagaagat gacattacca aattgcccag
    12001 aatgtggctc agagtacaca tatgaagatc gaggcttgtt gatttgccca gaatgcggga
    12061 acgaatggaa caaagaagaa caagaaactg tcatagaaga cggattagtg gtattagatg
    12121 caaatggaaa ccgtctgtta gagggcgata gcgtgacagt gattaaagac ttgaaagtaa
    12181 aaggtgcgtc aggtgccatt aaacaaggaa caaaagtcaa gaacatccgt ttagtagaag
    12241 gcgatcataa tatcgattgc aaggtagaag gatttggacc gatgaaactg aaatcggaat
    12301 ttgtgaaaaa gaattaaaga atattcaagg tcttttcaca ttcttttcct actactaatg
    12361 ataaaataaa gtaaacgaaa aggagtgaaa cgaaaatgta tggttatgga atgtatggtt
    12421 ttttagatcc gacatttctt ttggtcattc ttggattagt tatttccgga attgcgtcag
    12481 cgtacgtcaa tagtactttc cgaaaatacg atcaagtgag aagcaaaaac aatgtgactg
    12541 gtacacaagc agcacaatat atattacaaa gtcaacagat aaataatgtc ggtgttcaac
    12601 aaattgccgg agatctcaca gataactaca attcaggtaa taagatgttg agtttgtctc
    12661 aagcaactgc acagtctact tcagttgctg cgataggtgt agcagctcat gaatgcggac
    12721 atgcggtaca agatgcttcg ggttatgtcc cattgaagat tcgtgcagca cttgttccag
    12781 cagctaactt tggttcgatg atttcgttcc ccttgattat ggtaggtgta ttgcttagct
    12841 ggaactctac gttaatcaat atcgggattt tcgctttttc tttagcatta cttttccaat
    12901 tggtgacatt accagtcgaa tttaacgcgt ctcggcgagc aatccaaatt ttgagtgaag
    12961 gcggactact gacggaagaa gaagtaccga tggcaagaca cgtattattt gcagcagcac
    13021 tgacttatgt tgcagcagcg ctttcgacat tcctgcagct gttgcgcttg atcttattgt
    13081 tcggcggcaa ccgaagagat taaaataaag aagttgtgaa atcagcgctt tgatctgagc
    13141 taataccgag agttagctga ttgaatacca tttatttgga ataagggctc tttgtcaata
    13201 aggactgatg ggttgtacaa aatcaaatct gggtcagaat gaacctcatt ctggctcag
//
LOCUS       DAYYFU010000068        13000 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000068 DAYYFU010000000
VERSION     DAYYFU010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.072"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..767
                     /locus_tag="ACHA34_002308"
     CDS             66..767
                     /locus_tag="ACHA34_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFM8792908.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="ACHA34_002309"
     CDS             764..1885
                     /locus_tag="ACHA34_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFM8792909.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="ACHA34_002310"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="ACHA34_002310"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFM8792910.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="ACHA34_002311"
     CDS             3264..4172
                     /locus_tag="ACHA34_002311"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8792911.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="ACHA34_002312"
     CDS             4207..4479
                     /locus_tag="ACHA34_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFM8792912.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="ACHA34_002313"
     CDS             4490..5536
                     /locus_tag="ACHA34_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFM8792913.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5787..8396
                     /gene="clpB"
                     /locus_tag="ACHA34_002314"
     CDS             5787..8396
                     /gene="clpB"
                     /locus_tag="ACHA34_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFM8792914.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8547..9233)
                     /locus_tag="ACHA34_002315"
     CDS             complement(8547..9233)
                     /locus_tag="ACHA34_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792915.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(9211..9705)
                     /locus_tag="ACHA34_002316"
     CDS             complement(9211..9705)
                     /locus_tag="ACHA34_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8792916.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9725..10945)
                     /locus_tag="ACHA34_002317"
     CDS             complement(9725..10945)
                     /locus_tag="ACHA34_002317"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8792917.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11092..12927
                     /gene="lepA"
                     /locus_tag="ACHA34_002318"
     CDS             11092..12927
                     /gene="lepA"
                     /locus_tag="ACHA34_002318"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFM8792918.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT         4257 a         2378 c         2727 g         3638 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatcct ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt
     5641 tgttttttta agatttatat aagtcgagaa aaaaagtcaa aaaactatgt gtatattttg
     5701 accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt
     5761 acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag
     5821 aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg
     5881 cccatgtatg gaaaatcttt ttacaatcta atcattttgg tcgaaatttc tatacggatg
     5941 ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag
     6001 ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat
     6061 tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg
     6121 ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg
     6181 gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga
     6241 cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac
     6301 agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg
     6361 tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg
     6421 tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg
     6481 aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa
     6541 aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg
     6601 atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag
     6661 aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt
     6721 tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg
     6781 aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc
     6841 tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat
     6901 tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag
     6961 caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa
     7021 cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga
     7081 aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg
     7141 aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca
     7201 attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag
     7261 aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag
     7321 aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag
     7381 agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa
     7441 cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac
     7501 gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg
     7561 ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg
     7621 tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata
     7681 tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag
     7741 ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa
     7801 atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata
     7861 tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt
     7921 tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg
     7981 tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg
     8041 gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga
     8101 gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg
     8161 aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg
     8221 cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa
     8281 caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta
     8341 ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt
     8401 ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt
     8461 caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct
     8521 gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt
     8581 tcaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca
     8641 cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac
     8701 agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag
     8761 aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg
     8821 aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca
     8881 tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag
     8941 atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag
     9001 taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc
     9061 atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc
     9121 gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca
     9181 agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa
     9241 tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat
     9301 caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc
     9361 agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac
     9421 tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag
     9481 cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc
     9541 aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag
     9601 atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt ggttatataa
     9661 caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt
     9721 tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact
     9781 tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat
     9841 aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt
     9901 catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc
     9961 aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa
    10021 accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct
    10081 ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat
    10141 gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt
    10201 cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc cagtagaaca
    10261 gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat
    10321 tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt
    10381 gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa
    10441 ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt
    10501 tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat
    10561 aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca
    10621 tcaatccatg gttagcatat tctggataag ttccaactcc agtcacacca cccattttat
    10681 aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat
    10741 tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa
    10801 aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat
    10861 aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat
    10921 tttctctaaa atctttttga tccatttttt catctccatt tattagcttg ataacagtat
    10981 agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg
    11041 cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata
    11101 aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac
    11161 catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc
    11221 gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt
    11281 aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac
    11341 ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc
    11401 tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc
    11461 aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac
    11521 ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat
    11581 gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag
    11641 cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg
    11701 atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac
    11761 ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt
    11821 acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat
    11881 gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta
    11941 acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg
    12001 gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta
    12061 gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta
    12121 ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc
    12181 ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt
    12241 aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta
    12301 acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt
    12361 gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac gatggattat
    12421 ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac
    12481 tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct
    12541 ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat
    12601 gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa
    12661 aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga
    12721 caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt
    12781 tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag
    12841 cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa
    12901 atggacgagg acgaaccgaa gaaatagcaa gggtttgttt gaaaacagtg taaaaaataa
    12961 accatgttta catggatttg ccaccggttt gccaccggtg
//
LOCUS       DAYYFU010000069        12943 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000069 DAYYFU010000000
VERSION     DAYYFU010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.073"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="ACHA34_002319"
     CDS             complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="ACHA34_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFM8792919.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1195..3036)
                     /gene="dnaG"
                     /locus_tag="ACHA34_002320"
     CDS             complement(1195..3036)
                     /gene="dnaG"
                     /locus_tag="ACHA34_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFM8792920.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3218..5533)
                     /locus_tag="ACHA34_002321"
     CDS             complement(3218..5533)
                     /locus_tag="ACHA34_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8792921.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5718..6578)
                     /locus_tag="ACHA34_002322"
     CDS             complement(5718..6578)
                     /locus_tag="ACHA34_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFM8792922.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6637..7269)
                     /locus_tag="ACHA34_002323"
                     /pseudo
     CDS             complement(6637..7269)
                     /locus_tag="ACHA34_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            7436..8197
                     /locus_tag="ACHA34_002324"
     CDS             7436..8197
                     /locus_tag="ACHA34_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8792923.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8223..10814
                     /gene="mprF"
                     /locus_tag="ACHA34_002325"
     CDS             8223..10814
                     /gene="mprF"
                     /locus_tag="ACHA34_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8792924.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="ACHA34_002326"
     CDS             complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="ACHA34_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8792925.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12557..12913
                     /locus_tag="ACHA34_002327"
     CDS             12557..12913
                     /locus_tag="ACHA34_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8792926.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         3713 a         2708 c         2300 g         4222 t
ORIGIN      
        1 tttttctttt aaaataaacc aaacagtttt actgatttct cttctggtca atagtccacg
       61 cttattctaa gaaatctttt aattgttttg aacgagaagg atggcgcaat tttcttaatg
      121 ctttagcctc gatctgacga atacgttccc gagtaacacc aaatactttc cctacttctt
      181 ccaatgttct cgtccggcca tcatctaaac caaaacgtaa gcgaaggaca ttctcttccc
      241 gatcagtcaa tgtatctagt acatcttcca attgttcttt tagtaactca taagctgcgt
      301 gttctgctgg acttgttgct tcctggtctt ctatgaaatc acctaagtga gagtcatctt
      361 cttcaccgat tggcgtttca agggaaacag gttcttgcgc gattttcaag atttctcgga
      421 ctttttctgt tggaagatcc atttccgcac cgatttcttc tggcgtaggt tctcttccaa
      481 ggtcttgcag caattgacgt tggatacgta tcaatttatt aattgtttca accatatgaa
      541 ctggaatacg gatcgtacgt gcttggtcag cgattgctcg agtgatggct tgacgaatcc
      601 accatgtagc ataggttgaa aacttaaatc ctttgcggta gtcaaatttt tcaactgcct
      661 tcatcagtcc catgtttcct tcttggatca agtcaaggaa ctgcatgcct cgtcctacat
      721 aacgtttagc gatacttact actaaacgaa ggtttgcttc tgccagacgc tgtttcgctt
      781 cttggtcgcc ttcttcgatt ttcaatgcta acgctacttc ttcttccgca gttaaaaggg
      841 agacacggcc aatttctttc agatacatac gaaccgggtc attgattttt acaccggttg
      901 gcgcagaaag atcttctgtt tttgcttgtt cagctttttt ctcagcactc ttcaagctgt
      961 gaattgatgg atcaccattt tcatccacca cactgatacc tgcgtcttca atcttttgga
     1021 tcaatttgtc catttcatct gcattcaaag tgaatggtgt agcaagctga ttggataaat
     1081 catcatatac aacttgtcgt ttgggtttgt tttctttgat gaaagctgct actgctgctt
     1141 catacgtttt tttatctgtt ccatttgcca taaaagaagg ccccccttga gtagctattt
     1201 ggcttgtttt aactgtttcg ttaaattgat gatttcaatc gtcaattcaa gctcaagctg
     1261 tttgtttcca gttcgtctgg cttcttgctg catttgtttt tttaatgtta tttcatcagc
     1321 taagccagat ttgctgatca catctagcaa gtcttggatt tctcgtggcg aactttcttc
     1381 tgccaccggt aaatattcga tttccaccac caaccgcctt aaattttcat cttgcaaaaa
     1441 gtctaaaaac tctgcaaggt tgaattcata atgttgtgag acatatgcat cgaataaaaa
     1501 ataaacttct gcatatgcat catgaataaa ctgaaattct cgttctttta aaatattacg
     1561 gacagaaact tctttaaatg cacgatataa tagaatctgc tctgcctttt ctacttgcgt
     1621 caaaggtctc tttttccgtg cagtttcttg tctcgtaacc tgagtgtgtt gactttcctg
     1681 aggttcttga cgattttgct gatttgtcct tcgaagatcg cgcagttgtt tttgtatcgt
     1741 ctctctgccg atctgaaact ctgttgacag ttgagataga tacatatctt gttcgattgg
     1801 tgaaacaact cggctaagtt caactagtaa ttcttcgagg tattcgatct tgtctttttc
     1861 attatctaaa tttttccctt gttttaaata acgtgattta aaagtaaaga ctgtttcgcg
     1921 cccatgagcc actaattctt ggaaagatgc ttctccatac ttacggagat attcatcagg
     1981 atcaagcttt tctggaacga ctacgacaga aagtgctaat cgactgtttt gccgaagcaa
     2041 ttcaatccct cgattggttg cttcaaaacc tgcgttgtct ccgtcatagc aaaagaccaa
     2101 ttctttcgtc agcttttcca tttgttggat ctgttgagct gttagacttg tccccataga
     2161 agctaatcca gttttgatac cagcctgcca tgcagcaatc acatccataa acccttcgaa
     2221 caacaaaatt tctccgtttt tccggatatc gcttcttgct ttatcaagat taaataaaac
     2281 ttgtctttta ttgaacagtt ctgtttctgg actattcaag tatttgggct gatctttttc
     2341 ctgccctttt tcaggtgcaa gccagcgtcc tgaaaaaccg atcgtctggc cttgagaatt
     2401 acgaagtgga aacatcactc tttgataaaa acgatcagac atacggccat catcatgcgt
     2461 gacaaacaat ccagagcgtt ctaacaattc atttgaaatt gattcattct tgaatacttg
     2521 ctgaagaaaa gaacgctcat taggtgcaaa acctatgttg aattcttcaa ttaattctaa
     2581 agacaaccca cgactttgca aatattctaa agctggctgt ccagcttttg tatgcaatag
     2641 catgtgatga tagatttccg ctgctttttc atgagcagca atcagtttcc ccgtagcaga
     2701 ttcaggattt tggacagcta tagtacgctg acgccattga tcttctaacg gtatctgttc
     2761 gaagtctgcc acttttacca ctgcttctgg aaaacttaga ccttctagtt cttgaagaaa
     2821 agtaaacacg tttccgcctt taccacaacc aaagcaatga aaaatctgct tgtcttctgc
     2881 aacagaaaaa gacggtgtac gttcttcatg aaaaggacac agccccaagt aatttttacc
     2941 agactttttc aactggacat attgtccaat cacctcaacg atattcgttt gactgcgtac
     3001 tgtttcgata acctcttgag gaatccgttg agccattttt tcaccccctg ctctattgat
     3061 atcttttggg tttaacagaa gatatttcag gagaataacc ggtaaaaaat cgcactaact
     3121 gaaatcagtg cgattttaaa ggacacaatt atacatgttt gattgtatca tatttcattg
     3181 ctttttcaag tataacacat ttctatctca ttaagcatca atttgctact cttttcagta
     3241 actgattttg aatatcagcc ttccagtaca atactcgaag aacaatattt acgagcatga
     3301 atacaaagaa gtatcctacg ctctctccca accctaagtt tacttccaaa attccatgga
     3361 acagcattgc agctacatat atccctacga tcgtcgttac tgctaggaag atgcgcaatg
     3421 ggttgaaggg ataacaagct tttacaactg ccatgcaact gatcccaatc agcaagtaat
     3481 acatcaaagt agtagtttca gcatttcccc atgcgaatgt ttttccaagt agatacaccg
     3541 caatgatatt cactactaca agcaaggcat ttggtaaagc attaataagt gctgtaggca
     3601 aataacggta agtgattttt cttttatctt gttcaaaaga taaaaagaag gaaggatacc
     3661 cttcaatcgc taaatcaatc agcgtgattt ggatagggat aaacggaaat ccaattgctg
     3721 taacggcaca tataatcgat aaaataaatg aataaatcgt tttgataaaa aagatgcctg
     3781 agactttcgt cacattgttg accactcggc gtccttcaaa caggacttct ggcaatgtag
     3841 taaaatcaga gtccaacaat accagattcg caatctgacg tgttgctcca tcaccttctg
     3901 ccatagcaat gctgcaatct gcttctctta acgccaaaac atcattcaca ccgtcgcctg
     3961 tcattgcaac cgttctgccg ctttctttca attcatttac cagcaatttc ttttgctgcg
     4021 gactcactcg tccaaaaacc gtatagcgat gagcagcttc tcgcacttcc atttcttcag
     4081 taatttgaga aagatcgaca tacgcatcat aattcggtaa tcctgcacgt ctagcgatat
     4141 tggagacagt aaaaggattg tctccagaaa tcactttcag atccacacct tcctcatgaa
     4201 gataagctag tgtttcttga gcgttttttc ggattggatc atcgatttca aacagagcaa
     4261 ccggctcaac aaatgctggt ttttcttcgt tcaattgctg atttggagcg atccccaaca
     4321 taagtacgcg atagccattc gcttgggctg tttttagact gtccggaagc cgatcttctt
     4381 ccaccaaacg ttcaggcgct cctacgtaca cgactcccag ttctggaaat tccatcgcgc
     4441 cccatttgcg atcagaagaa aatgcgactt tgtttgtcac atcataccga ttagaaagct
     4501 caaaataatc tctcatagcc cgcatagtga tgttgttatc actgctttct gccagatacg
     4561 tccccataat ttctgggatg gctttctcgt acagcggatg tagtgcttct acccgctgga
     4621 tactcatttt cccttcagta atggtccctg ttttatccaa gcataatgta tcgacatggg
     4681 ccagcgtttc aattgaatac atgtcttgaa caaggattcg tttttttgcc agtttggtta
     4741 cccctgtcgt taatgcaata cttattagca ataccaaacc ctttggaagc atgcctaaca
     4801 aagctgctgc agatgcaata acagaaattt tgatagaagc atcgcggatg aacagtgctt
     4861 ccaaaaatag tatgatcccc aaaggaataa tcacgaaact agtgaatttg gatactttgc
     4921 gaatcgaatc tacaagttca gaacgaatag ctttgtgagt ctttgcctcg ctagtcaagc
     4981 ggacagcata attatctgca cctactcgga taacttcagc catgacagaa ccgcttgtca
     5041 aaaagcttcc agaaagcaat tcagctccag tttctttgac aatcaaatca gattcgcctg
     5101 tcaaaagagc ttcatttgct tctgcccgtc cagatataac ctgtaaatcg gctggtatct
     5161 gttctcctgc tccgattttt accaaatctc ctaataccag ctctttagta gaaatcgatt
     5221 gttctttacc gtcccggatc actagtactt ctgattggga aacaatcgat aatttccgta
     5281 caagatttct tgcatgtatt tcttgataaa ttccaataac gatattgacg acaataattg
     5341 ctaaatacgc catgttggaa tacgcaccta caaataacag acaaatccca ataccaaagt
     5401 tcagaaaatt gaataacgtc aatatattat cgctgaatat ttgttttgta ctttttgatg
     5461 catcttcctg gtagtcattt tgctggccag aagccatttt ctgaactacc tcttgctgtg
     5521 taagtccatt cattttcttt ttctccattt tccgccaact ttcaaaaata atcacgtgtt
     5581 taagtctagc gtgttccaag attaaaaaca acttgttttt ataaaattta tagaaaaaca
     5641 cacgaatgat tttacaaaaa agatgccaac ctttttgttt gaatcacccg tgtgctgttt
     5701 ttattatcaa tctatcttta cttatattgt tcctccaaac ggctcatcca ccaacctagt
     5761 gctgtaccgg ctataaaaag aacaggacaa gctagtaccc cagcaactcc caatcccgcg
     5821 taatcggtag gaatgatcat aatcgttgaa gcaaaaacta cacctaaaat gaaatggaat
     5881 agcttgctat atgcctttga gaaaatgtaa tccatgattt ttgacaatcc aagaacacaa
     5941 agaatcccac caattccgat cggcacaatc acttcaagtc gtacttcttt aaatccgtca
     6001 gccatcgctt tatacaatcc catatacacc aaaaaattag aagggctcaa tcctgggacg
     6061 atcataccca aaccaatcaa tccgccggca atcagccatg ttccaaaatt ctgatcgact
     6121 tgcccgtcaa ataattgagc gccgtataat aaaaacaaat aagccagaac aaaagtaaca
     6181 accatgatga tcacatcaga agtatctctt ccttttttcc ctgcttcttt ccataacgca
     6241 ggaacagttc cagcgataca tccgacaaaa aaccataata tcgttgtttc atacgcacct
     6301 aataaaaaac tgactaaaaa agaaagtaga aagatgcctg tcactccgcc tagaccaaca
     6361 ggcagaaaaa acaaaacatt ttctttgaaa ttttttgtaa ggtgggctaa aaaagaaatc
     6421 agccgctcat acatgccaaa gacagctgct aatgcaccac cgctgactcc tggcaagata
     6481 aatcctgaac cgataaacat tcccttcaca aaccgtaaca tccagtcttt ggttgtgtac
     6541 cccacttctt gattttccat tttttgcctt ttttccacta tttgcgcact ccttctttca
     6601 tttaatttta ttcatgattg aacatttcct gctattttac ttaaagactt tttcataggt
     6661 ctctattatt ttttcattca ttttgacaat atcaggataa aacatgttac ctccattatg
     6721 atagagatag ccgccattgt aaagcaagga ctggatccgc caatatcggt attgtttccc
     6781 ttcctcattt cctaaaagtg ggctcagcac atctttagaa tacgcttctg ctaattctac
     6841 tgtgttttct ccgccatgat tttttacata ggcaatataa tctttcccaa aattatatgc
     6901 ttggacagct gtccataaat cgcatccttg tttctttgct tctactatca tttccgccaa
     6961 atatttgaca ccttgatcaa tactgtcagc tggattatca atgatctcag cttctccatg
     7021 cagactttcc gagctctgca tgagatctgt cccttttcct tttgattcgg tcaatattat
     7081 cgccttgatc aaattctcgt agttttccat atgataattt tttgcagctg ttgttatttc
     7141 tatttgatag ctttccactc gttttaccga acgataagtt cttcctcata aaaacaacac
     7201 cgcaccaata accgtcagaa tgatcatgta aaaaagaata cggataatag ataatcgttt
     7261 tcgcttcatt tcatcaccta gttattttga catctttttt tatcttacta tttctttatc
     7321 ttcttggcta tttcttcctt gatttctgtg tacgccttaa gaaaaaatga attttcctgt
     7381 attttttgac atcttacgaa aatgatcgtt gtttttcagt aaaaacaatt gcccaatgac
     7441 tgatataatc gttatacttt acacagtagg tgattatgtg aaaaaagaaa agaaatatgc
     7501 aaaaatgcca gatgggagcg aaatttacta tgaaaagagc ggtcaaggct tccctctctt
     7561 tttactgcat ggcaatgacg gcagcggccg ctttttttca gaacaggtcc ctgtactcga
     7621 acgctactat accgtgtatc tagtagatag ccgaggacat ggtcgttcga caaacgaagc
     7681 atctatgttg aattttcagt taatggccga ggatctgaat acgatcatgt tgctggaaaa
     7741 aatcgatcag gcagattttc ttggatttag tgatggagca aatctagcac tggtatttgc
     7801 cagcagcttc cctaaaaaag ttcatcgatt gattttaaat tctggaaaca cgcttgttaa
     7861 aggcgttcgt ttttctgcca gagtcatcag taatattcat tatgcatggg tatggttact
     7921 ctctttattt cgcccaagtc ttcgcaaaaa tcttttagtt atcaaacttt tgctgcatga
     7981 catcgggctt acagaaaatg atttaaaaaa aatcaactcc cccactttga tcatcgtggg
     8041 aaaaaaagat gtaatcaagc taaaacattc tttgtatata gctaaaacta taccaaaagc
     8101 ttcctttgtg cttgtgaaag aacaaggaca tgaattagct agaaaagatc ctgaacgctt
     8161 caatcgagaa gttcttcaat ttctttctga aacataacta ttaggaatag aaggtgattt
     8221 atttgttaaa aaataccata caatggctga aaaaccacct aggactgttc aaaacaatct
     8281 ttttgatttc tgtcattgtc attattgtcg gtgaattgat gtccatcggt aaaactctat
     8341 ctatccagca attaggagaa acgtttgcca ctatacctgt atggaaaagc ggactaatgc
     8401 tattgattgg tcttatctct gtgttaccta tggttggtta tgatattatt ttgaatcgga
     8461 tgcttgaaca aaagcagaat cctcgttacc tgtttgagac aagctggctg atcaatacga
     8521 tcaacaatat tgccggtttc ggcgggtttg ttagtgtggg gcttcgctct gaactatatg
     8581 gacaaaagaa agagagcaga aacgtgatcc aagcattatc gaaagttttc ttgtttttga
     8641 tggctggact ttctatttac agtctgcttt cttttttact tgtagtcttc acgccagttg
     8701 ctcctttttt gaagcaatat tggatatggt tgatcggcgg cggtctatat tttcctgtcg
     8761 tctttttatt tactactttc aaaaagaaag gattccttgg tggtacctca cttaaaactc
     8821 aaggacagct attgctagtt tcattgttgg aatggaccgg tgtcctgttt agtttccttt
     8881 ctgttggtta tttaatggaa atccggattg atctgtggca aacaattcca ttattcattg
     8941 ctgcttcagt gataggaatc gtgtctatga ttccaggaga aattgggagt ttcgatgtaa
     9001 tgatgattat cgggttgtcc gctattggtg tgccaagaga aatagtcgtt gtgtggatct
     9061 tgctctatcg cctgttctac tacatcattc cgtttttgat tggaatcgtc tttttcttca
     9121 aaaatatcgg ttccactttt gatcagcgtt attctggtat ccctaaacaa ttagcaacag
     9181 aaattgcgca taaaatcgtg gttgtcttat tatacttttc tggaatcatg ctagtacttt
     9241 ctgccactat cccgcaagct tttacagagt ttcgctggct tcatagtttg aacccattga
     9301 aatttcattt tattatccaa tttcctagta tactattggg atttctttta atcattatgg
     9361 gcagagggat cgcagcacgt gtgaaacgag catatttgcc tacaatcttc ttgattgttc
     9421 tagctttact ttatgtttta ttaagtgatt ttagtttcac accggtggtt ttcttgtcta
     9481 ttctgcttct gatcattctt gcttctaaaa acgaattatt tcgggaacaa ttgatctatt
     9541 catgggagtg gcgtacgatc gatggaatca ttattggtgc actgtccttg ctttacatcg
     9601 ttattggggt atacaatctg ccggatttcc ctcatcggcg tcaccatttt atttcctttt
     9661 tcttatttcc ttctgaaaag atttggtttt cgggattgtt ggccatcatc gctgttagtt
     9721 tcatgatcgt tctatttgtt catttccttc aaggagaaaa gaaacagatt ggcgaagcct
     9781 ttaatgaaga aaaggccctg aagatcctta caacttatgg aggaaactcc gatagtcaat
     9841 tgatcttctt aaaagataag cggatgtttg cctatgaaaa agacggtgaa ccaactgttc
     9901 ttctccaatt cgcttgcttc aataataaat gtattgtaat gggtgatcct tctggtaaga
     9961 aagaagattt tccagaagca atcgaagctt ttatcgaaga aacagatcga ctatgctatt
    10021 taccagtttt ctacgaaaca agcgaagaga ttgttatgat tttgcatgaa ttcggctacg
    10081 atttcattaa gatgggggaa gaagcttatg tagacctgaa tagttttaca acttcaggga
    10141 aaaaaatgaa gggcactcgt gctgtcttga atcggatcga acgagaagga tttacatttg
    10201 atgtcttaca accaccattt tctactgagc aaatgagtac tttcaaaaat atctctgata
    10261 attggttggg ttcacgaaag gaaaaagggt tttctttagg ctttttctct gaagattatc
    10321 tacaaagagc gcctattgca gttgtcaaaa atacccaaga agaaattgtc gcttttgcaa
    10381 cgatcatgcc tacttacacg aacaataaag taggaacgat tgatctgatg cgatatgatt
    10441 ctaaaaaagc accttctggc agtatggatt tcttgttctt gcatttgttt gcctatatga
    10501 aagaagaaca tatccaatgg ttcaatttag ggatggcccc tctttcaaat gtcggaactt
    10561 ccagaaagag ttttttacag gaaaggattg catacttggt ttacgagttt ggttctcatt
    10621 tttattcttt ccatggatta agagaatata aaagcaagta cgctacaaac tgggtctcac
    10681 gttatactct atattctaga gatagttgga ttgcatatgt tatgattgct ttgctgatta
    10741 ttgataatgc gccagttgag cagacacaag gtagaataag tggtttgaaa aaatggatac
    10801 gaagaaagta ttaaaaactg cataccgata gatgaacact ctcatgatga ataaaagaga
    10861 ctgaagtaaa acgaaagttc atgcttctac aatctcttct tatacaattg aagaacaaag
    10921 agcggtccaa acagaaatcc tgtttggacc gctcttttat tttaaacatt tttcattacc
    10981 aaaatattcg agtcgcgttt cttatgcttc gatctctgta acgatacctg aacctactgt
    11041 ccgtccgcct tcacgaattg agaatgtcgt tccgttttca acagcgattg gatggatcaa
    11101 gtcaacatca atcgtcacgt tgtcaccagg catgaccatt tctgtatctt ctggcaatgt
    11161 gattgttcct gtaacatcag ttgtgcggaa gtagaactgt ggacgatagt tgttgaagaa
    11221 tggagtatga cgtccacctt cttcttttgt caacacgtac acttctgctt tgaattttgt
    11281 atgtggagta atggaacctg gtttagcaag tacttgtcca cgttcgatat cgtcacgttg
    11341 gatcccccgt aacaacacgc ctacgttatc cccagcttcc ccgtaatcta acgttttacg
    11401 gaacatttct acacctgtta caactgcttt ttgtgtttca ggtttgatcc ctacgatctc
    11461 tacctcatca ccgacacgaa cagcaccacg atcaatacga cctgaggcaa cagttcctcg
    11521 acctgtaatt gagaagacgt cttccactgg caatagtaat ggtttatctg tatcacgttc
    11581 tggtgttggg atatattcat ctactgtatc catcaattcc ataatagcag cttcagcatc
    11641 tggatcgcct tgcaatgctt ttaatgcgga acctttgatc acaggagtgt catcgcctgg
    11701 gaaaccatat tcgctcaata actcgcgaac ttccatttct accaaatcga tcaattcttc
    11761 atcatcgact aaatcaactt tgttcaagaa aacaatcagg tattttacac caacttggcg
    11821 tgacaataaa atgtgttcac gtgtttgagg catcggaccg tcagttgcag aaacaactaa
    11881 gattgcacca tccatttgtg ctgcccctgt aatcatgttt ttcacatagt ccgcgtgtcc
    11941 tggtgcatca atatgcgcgt agtggcgttt ttctgtttca tattctacgt gtgcggtatt
    12001 gatcgtaatc ccacgttcac gttcttctgg agctgcatca atactagcat agtcttgagg
    12061 atttgctagg ccttttttgc ctaatactgt agtgatcgca gcagttaaag tagttttccc
    12121 atggtcaacg tgtccaatcg taccaatgtt aacgtgtggt ttacttctgt cataatgttc
    12181 ttttgccata atttacaaac ctcctaaata atatagatca ctgaaaaatt tcagctctca
    12241 cctttttatt atagtctttt tttgactaaa tcaaaaagaa tacgtccgac ttactttttg
    12301 taagattcga actaagctta ttatacgtca atattggtca aagtcaacta agagcactcg
    12361 tctgtcttat acgcgaaaaa agtacctttt ataaataaaa aaatgtgttt tttgagaaaa
    12421 acttcaagat agtccttaac tttcttttga agcaataaga acaaaatgag gaaaagttta
    12481 aaattcagct gttgccaaca actttcaata gacgaaatct tctatttcgt taataataag
    12541 gaaagaggtg aagtacatga aaaaattaat tggcgtactt gtcgttttag gcatatttct
    12601 cggcggaagt tttgcagctt atcattattt ttacggcggc gaagcttatt atacaaaaat
    12661 cacgacgaat ggcgaaatgt cagctaccca agcagataac ggagagaaat tcacgactta
    12721 tacctacaag caagcggctt ataataaaga tggtgaaaaa aaacaggtga cgcttcgcga
    12781 agaaagagaa catccattaa agatgaatgc gtatcttaaa ctcaaagtca atcctcgtaa
    12841 aggtgtatta agctgggaag aaataaaggc ttctgaagtg cctaaaaatg cggcagaaaa
    12901 aatcgataat tagtaaatgg tcttaaaatt ttattagata aaa
//
LOCUS       DAYYFU010000070        12734 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000070 DAYYFU010000000
VERSION     DAYYFU010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.074"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACHA34_002328"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACHA34_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8792927.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ACHA34_002329"
     CDS             complement(322..891)
                     /locus_tag="ACHA34_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFM8792928.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ACHA34_002330"
     CDS             1077..1979
                     /locus_tag="ACHA34_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8792929.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACHA34_002331"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACHA34_002331"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFM8792930.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACHA34_002332"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACHA34_002332"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFM8792931.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACHA34_002333"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACHA34_002333"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFM8792932.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ACHA34_002334"
     CDS             8547..8732
                     /locus_tag="ACHA34_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFM8792933.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ACHA34_002335"
     CDS             complement(8794..9990)
                     /locus_tag="ACHA34_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8792934.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="ACHA34_002336"
     CDS             complement(10160..11596)
                     /locus_tag="ACHA34_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8792935.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="ACHA34_002337"
     CDS             11889..12698
                     /locus_tag="ACHA34_002337"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8792936.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DAYYFU010000071        12107 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000071 DAYYFU010000000
VERSION     DAYYFU010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.075"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..694
                     /locus_tag="ACHA34_002338"
     CDS             <1..694
                     /locus_tag="ACHA34_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFM8792937.1"
                     /translation="LVTVAKRPLDRIDQEAVFCQELAIQGTLLPLTETYLKEYFPELH
                     ATEAFTVLQAFFLSRVINHLIAPIQNKIMAETIEKMKQDSKEIYAKNNSFLVASLSEL
                     KITDTYLPDIATSLTLLVRRLKERYLQDRKKIIFLLEGDLYVCQSIRSRVLHYFGKRH
                     DLFFMTIQALSDEFLAQEDIDLVVTNYSDYLSEYTVNTDYLLLKTVPDSQDWRLLFQK
                     IEPQLSTLIGLN"
     gene            complement(752..1747)
                     /locus_tag="ACHA34_002339"
                     /pseudo
     CDS             complement(752..1747)
                     /locus_tag="ACHA34_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558311.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            1860..3062
                     /locus_tag="ACHA34_002340"
     CDS             1860..3062
                     /locus_tag="ACHA34_002340"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006278 - RNA-templated DNA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
                     /protein_id="HFM8792938.1"
                     /translation="MLVKYKVNDAGSRPVKIKPRELYRASHLDSCIYRYYAECLNKDY
                     EKVAEIRRIGDNVVAYRVGKGKSNIDYAAEAIDFIKRSNDGCYIFLGDFEKFFDTLDH
                     EYLKCKLKEVLSMSTLDEDWFKIFKTITSFSWVEQKDLNDLFGSEKEQYKNGLKSYFD
                     TWEEFRNFKADGHVCKNTNSFGIPQGTPISALLSNIYMIDFDDWLNKLSSKYGGKYLR
                     YADDFILILPFSTTSKDIFLEIIREILQYASEVTKLKIERNKTEKYLFTNDSVYLFES
                     NKNSTSSLVKSKLDYLGFVFDGSQVKMRQKSVSKFYRKADKAIRAAREKSRIKKSSYL
                     IGKRHIYRYFFDVGVRDSKGSKFGNFISYARRCQMKFDQISPLTENLMMEQIKNRRKK
                     LLKKMYKN"
     gene            complement(3227..3460)
                     /locus_tag="ACHA34_002341"
     CDS             complement(3227..3460)
                     /locus_tag="ACHA34_002341"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792939.1"
                     /translation="MSQLNQTYENYLQQPEALSFTEMTTIYQEILKNADMNDKDFIEI
                     WEELMGAAIRYTVILGHHAYSYKKNKNNVQEVM"
     gene            complement(3719..3913)
                     /locus_tag="ACHA34_002342"
                     /pseudo
     CDS             complement(3719..3913)
                     /locus_tag="ACHA34_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367047.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(3974..5487)
                     /locus_tag="ACHA34_002343"
     CDS             complement(join(3974..5326,5329..5487))
                     /locus_tag="ACHA34_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179228.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8792940.1"
                     /translation="MYTYEERKKAVDLYFKYDQALAATTKKLGYPSVGALRQWIREFQ
                     SSDKLHKNIRTSWKYTLAQKEVAVNHYLDYGQCYARTIRKFGYPNKTTLRKWLEEYAP
                     STRKVRQNSIHYTKQEKEAAVLALCTRSLSAKSIANDFGVTRQALYLWKNKLLGKGAT
                     KPLKSMNVTPEIKLLKEERDILQREVYKLRLEKEILEQASDLIKKEPGIDPIILTNKE
                     KTQVIDALCSKYSVIELLRILDLPKSSYYYHRKQLAIPDKYLKERSLIVQLFHDNKGR
                     YGYRRIYCSLKLLGITLSEKVVRRIMTEEKLKVKSLRLRKYNSYGGEITPAVPNLLKR
                     DFKADQPNKKWLTDITEFHIPSGKVYLSPMIDCFDGAIVSWTISTKPNADMVNSMLEN
                     AIATLNAKDSPIVHSDRGAHYRWPGWIKRMKEACLVRSMSKKGYSPDNSACEGFFGRL
                     KNEFFYGQDWKSTSIDQFITDLDNYILWYNKKRIKCSLGGLSPLDYRKKLGIL"
     gene            complement(5810..6634)
                     /locus_tag="ACHA34_002344"
     CDS             complement(5810..6634)
                     /locus_tag="ACHA34_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8792941.1"
                     /translation="MEIKVLFENSRINLNFKSGYGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTEIAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGIPYINNL
                     GLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQHLEIS"
     gene            6977..7393
                     /locus_tag="ACHA34_002345"
     CDS             6977..7393
                     /locus_tag="ACHA34_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8792942.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIRESQQSVIDCRKLLVEQLTGGDDL"
     gene            7390..7860
                     /locus_tag="ACHA34_002346"
     CDS             7390..7860
                     /locus_tag="ACHA34_002346"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8792943.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            7874..8647
                     /locus_tag="ACHA34_002347"
     CDS             7874..8647
                     /locus_tag="ACHA34_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8792944.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            8661..9473
                     /locus_tag="ACHA34_002348"
     CDS             8661..9473
                     /locus_tag="ACHA34_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8792945.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            9527..10492
                     /locus_tag="ACHA34_002349"
     CDS             9527..10492
                     /locus_tag="ACHA34_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8792946.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            10629..>12107
                     /locus_tag="ACHA34_002350"
     CDS             10629..>12107
                     /locus_tag="ACHA34_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8792947.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQE"
BASE COUNT         3950 a         1957 c         2274 g         3926 t
ORIGIN      
        1 tttagtaacg gttgctaagc ggccgttgga tcggattgat caggaggcag tcttctgtca
       61 ggagttggcg atccaaggga ctttacttcc attaacagaa acgtatttaa aggaatattt
      121 tccagagttg cacgcaacag aagcatttac tgtcttgcaa gcattctttc tttcccgagt
      181 aatcaatcac ctaatcgccc ctattcaaaa taagattatg gcagaaacga ttgagaaaat
      241 gaaacaagat agtaaagaaa tttatgcgaa aaataattcg tttctggttg cttctttatc
      301 ggaattaaaa ataaccgaca cttacttgcc tgatattgct acgagtttaa cgttattagt
      361 cagaagattg aaagaacgtt atctacaaga tagaaagaag attatctttc tgcttgaagg
      421 ggatttgtat gtctgtcaaa gtattcgttc aagagttctt cactacttcg gcaaacgtca
      481 tgacctattc tttatgacga ttcaagctct aagtgatgag ttcttagcgc aagaagatat
      541 tgatttagtc gtaacgaatt acagcgatta tttgtctgag tacacagtaa atacagatta
      601 tctactatta aaaaccgtac cagatagcca agattggcga ttgctattcc aaaaaattga
      661 accacaactg agtacgttga ttggtctgaa ttaacttgaa gcctatttaa cttttttcgt
      721 taagtaggct ctttttttgc gtcttgaatt gtcaaatcaa gtgcaacact ttattaaaga
      781 agacttcata aggtgttttc caacctagac actttctagg tctgctattt agtttttcaa
      841 ccatccgata aatcgttgcg tcatctaccg tgtccatctc ttttgatttc ggtaagtact
      901 ctcgaatgag tccattcgta ttttcatttg ttcctcgttg ccaaggggca tgcggatctg
      961 gaaaataaaa gggcacatta ttcaatgctt ctgtaaccgc gaaatgattg gagaattctt
     1021 ttcctctatc tggagtgatc gttttgacca catcattggg gatactgctt aaaagttcaa
     1081 tcattttttc gctcacgtaa gcagctttct ttcttggtac ctttccagct aaaaggaatc
     1141 gcgattttcg atctgtcaac gtcactaaac aagcaccacc agtttttcca gctacagtat
     1201 cggcttccca atgtccaaat tcacttctat cgtctgcaga ttttggacga tcatgaatcg
     1261 aatgcgtaat gcgtattttt ccacgagcct cgacatgatt ttttgtatgt cttgtttttc
     1321 ctcgatggcg taatgaacgg atcagccctc tattgccact cgagagcggt tctgtatcga
     1381 aaataccgtt gtaaatggca cggtagaccg tgataaaact gattgagaag tccacctgtt
     1441 caagcttcaa acgattagag atttcctctg gcgaccattg aaattctaca aataaatgac
     1501 gaacgattga ccaaattcgc ggatcattta gaatagaatg acgccctcag tcctttctac
     1561 gagaagcata ctttttgtct gctttggaag gtgagtaggt tttctccttg tttcttctca
     1621 attcacgtga aatggtagac ggactacgat gaatgtcatt gccgattttt ctaatagaaa
     1681 caccttcttc atgaagtaga aagattcttt ctcgttcgag tatggtaaga tgatggtact
     1741 ggctcataat tgtcttcctc ctagtagtgt tctcgacaaa cctattctag ttggatattg
     1801 attatgagtc attttttagg tgttgcactt aaagtgtaaa ttcaaaacac aaaaaaaagg
     1861 tgttagtaaa atataaggtt aacgacgcag ggagcaggcc tgtcaaaatc aagccgcggg
     1921 aactttacag agcttcacat ttagattctt gcatctacag atactacgca gaatgtctaa
     1981 acaaagatta tgaaaaagta gcagaaataa gaagaattgg tgataatgtt gttgcttata
     2041 gggttggaaa aggtaaatca aacatagact atgcggctga agcaatagat tttatcaaaa
     2101 gaagcaatga tggctgttat atttttttag gagattttga aaaatttttt gatactttgg
     2161 atcatgaata tttaaagtgc aaattaaaag aagtcttaag tatgagcaca ttggatgaag
     2221 attggtttaa aatttttaaa acaataacta gtttttcttg ggttgaacaa aaagatttaa
     2281 atgatttatt cggttcagaa aaagaacaat ataagaatgg tttaaaatct tattttgata
     2341 cgtgggaaga atttaggaat tttaaagcag atggtcatgt atgtaaaaat actaacagtt
     2401 ttggaattcc tcaaggaacc ccaataagtg ctttgctatc aaatatatat atgatagatt
     2461 ttgacgattg gctaaataaa ttatcatcca aatatggtgg gaaatattta agatatgcgg
     2521 atgatttcat tttgatactg ccctttagta caacctctaa ggacattttt cttgaaatta
     2581 tccgagagat tctgcaatac gcatccgagg tcactaagtt aaaaattgag agaaataaaa
     2641 cagagaagta tctttttaca aatgattcag tatatttgtt tgagtcaaat aaaaatagta
     2701 catcaagtct agtaaaatca aaattggatt acttgggatt tgtatttgat ggaagccaag
     2761 taaagatgag acaaaaaagt gtttcaaaat tttatagaaa agctgataaa gcgatcagag
     2821 ctgctagaga aaaaagtcga attaagaaat cgagttatct aattggtaaa aggcatattt
     2881 atagatattt ttttgatgtg ggtgtaaggg atagtaaagg gagtaaattt ggaaacttca
     2941 tttcctatgc tagaagatgt caaatgaaat ttgatcaaat atctcctctg acggagaatt
     3001 taatgatgga acaaataaaa aacagacgta aaaagctatt aaagaaaatg tataagaact
     3061 gataatcaaa tgatatgctc ccatcatagt agacgttaga aaaaaatatt gataatctac
     3121 tatgatgaga atttatcaaa tcgtgacttt catatctatc gtccattaac cgctgaaaca
     3181 aacaccaaat aattcgcgaa atcgccaatc cgttgtcggt ggtcattcac atcacttctt
     3241 gtacgttgtt cttgttcttc ttgtaagaat aagcgtgatg tccaagaatg actgtataac
     3301 gaatagcagc acccatcaac tcttcccaaa tttcgataaa atccttatca ttcatatcag
     3361 cattttttaa tatttcttga tagatagtgg tcatttcagt aaaagatagc gcttctggtt
     3421 gttgtaaata attttcgtat gtttgattca attgggacat taaatcactc cttgaaatgt
     3481 ttttgtttta actagtttac tacaagaaat aaaagttagt gcatcaaaag aaaaacagga
     3541 aagcatacca ttttggtagt tttttctcgt tgagttggta taaatttgat gagacaaata
     3601 ggctagtatc actttattca ttgatactag cctattttgt atccaaaatt tatgaaaatg
     3661 attaaaacaa atctttttct tgctcaatga attgagcaaa tttatcagca cttcgttctt
     3721 atcgttctgg ataaaaatag ttgtagactt gttttgtcat ttttgaatct ttatgtccaa
     3781 cacgatacat aatatcctat agtttcatgt ctgccattgc gcacaaggaa acgtgagttt
     3841 ttctaaattc atgaagagtt atccgttttt caaggtgata tttattagcg atcatgtcat
     3901 accaatcatt tggtgtcaag ggcatcttaa aattgtaggt ttgggggtgc ggacgttttc
     3961 ctgcaccaaa attttataaa atacctagtt tcttacgata atctaatgga cttaatccgc
     4021 ccaatgaaca cttgattcgc tttttgttgt accaaagaat gtagttatcc aaatcagtga
     4081 taaattgatc aatagatgtc gacttccaat cctgcccata aaagaattca ttcttgaggc
     4141 gcccaaaaaa gccttcacaa gcagaattat caggagaata tccttttttt gacattgaac
     4201 ggactaaaca ggcttctttc attcgtttga tccaacctgg ccaacggtaa tgtgcgccac
     4261 gatcagaatg aacaattgga gaatcctttg catttaatgt ggctatagcg ttctctaaca
     4321 ttgaattgac catatctgca ttgggttttg tgctaattgt ccaactaacg atagctccat
     4381 caaaacaatc aatcattgga gaaagataga ccttcccaga gggaatatga aattctgtaa
     4441 tatcagtgag ccatttcttg tttggttggt cagctttaaa atcacgtttt aaaaggttcg
     4501 gaacagcagg agtaatttct cctccataag aattgtactt tcttaatcga agtgatttca
     4561 cctttaattt ctcttcagtc ataatgcgac gtaccacttt ttcagataaa gtaattccta
     4621 ataacttaag cgaacaataa atacgacgat agccatatct tcctttattg tcatgaaata
     4681 attgcacaat taaggagcgt tcttttaaat atttgtctgg gatagctaat tgtttcctgt
     4741 gatagtaata gctacttttg ggcaaatcaa gaattcttaa tagctcaatt acgctatatt
     4801 tggagcataa ggcatcaatc acttgtgttt tctctttgtt tgtcaatatg attggatcga
     4861 tgccaggctc tttttttatt aaatcagacg cctgttctaa aatttctttt tctaagcgca
     4921 gtttgtatac ttcacgttga aggatatctc gttcctcttt caacaatttg atttcaggag
     4981 tgacattcat tgatttcaaa ggtttagtag ctccttttcc aagcagcttg tttttccata
     5041 agtacaatgc ttgtctggtt acgccaaaat catttgcgat tgatttagca gagagtgaac
     5101 gagtgcacag ggctaggaca gcagcttctt tttcttgttt tgtatagtgt atcgaatttt
     5161 gacgaacttt acgagtagat ggagcgtatt cttccaacca ttttcttaaa gtagttttat
     5221 ttggatagcc aaactttcga attgtacgtg cataacattg cccataatct aaataatgat
     5281 tcacagcaac ttctttttga gcaagcgtat atttccaaga agtacgttga tatttttgtg
     5341 cagcttatct gaagattgaa attctctaat ccattgtctg agggcaccaa cagaaggata
     5401 tccaagtttt ttagttgtag cagccaatgc ttgatcatat ttaaaatata aatcaacagc
     5461 ttttttccgt tcttcatatg tgtacatgag acgtcctcca aacttatttc agtccaagtt
     5521 tttgtccgca tgcccttgta tgtactttta ttctgccata aacagcttaa tgaatgtcca
     5581 cttgaaaaag tgaataaacg aaatagatga ctcgatttaa taaggaagta aacccacaca
     5641 gaaatatagg tgattttcct tgaatgacca agatattgaa agttagtgaa aaaacacaaa
     5701 aaggtagccc tattcacaga aggtctgcct tttgtgttat cgccagcaaa cattagaggg
     5761 gatatttcat atagcttttg tcctctcgac agtcctatca gtttctcttt taacttatct
     5821 ctaagtgctg tccagtttta atagcttcta gcttgtttcc taactgtgtt tttaatatgt
     5881 tgaaggcttt tgaacctgtg cagtggcaag taaggatttt ttctactcgt gagtcattca
     5941 atttatttcc aattttcatt acctcttgct caggtagacc taaattattt atataaggta
     6001 tgccaatcat atggaagcca ccgattaggt attttatggg tttagtcgga aatgtttcaa
     6061 gagcaatttc catcatatcg ataatgccat gatgactaca gccagtaaag acaatcagac
     6121 cgtcttcttg ctcaattacg accaataatt catctgaaaa atcatcataa acaaatttat
     6181 catccatctt tttgtataaa ttcttggtgg tcgagtttgc tttgcctagg gggatgatat
     6241 gaaagctcgg agcaatttct gttttcgttt ttattgtatg taaattctca tgcattttaa
     6301 ggatttcatt tttaggaata ccaacataca gaaacgtggt taaaatcttt aaataatggg
     6361 gtaaagttac accttcagct gtataaatag gggcgtttcc tgattttaaa tatcttaacc
     6421 cgccggtgtg gtcagaatga ccgtgtgaaa taatcactgc ctccagatca ttcaaagttt
     6481 cccccatttt agtcgcatta tagactaatt ttttattagc gccagcatcg aagagatatt
     6541 tcttccctga gtagttaatt aagagagata acccatatcc acttttaaag ttcaaattta
     6601 ttcttgagtt ttcaaataaa accttgattt ccactataac atctcctttc acatgaacgt
     6661 gtattcatat attgtagcac attaagttct attttttgtt gtaattaccg aaaaaaatat
     6721 taatggacgc tttttacgct ttatttcgta tgtggaagaa aatttcttag gatgtggcgg
     6781 gggattgggg caaagctaaa ttccaattac agtgatcatt taattagaca cctttaaata
     6841 cccacacttt catcaacact ataatttagg gaaagaaaag tgttggcatg atatagaaaa
     6901 acctttaatt aacagcgttt cctaaaattg gcacgcatct tgcttataga aaagcgtaag
     6961 gataggagga atgaaaatgg caactataat tcttgccagc catggggaat ttgctcaagg
     7021 attaaagcaa acagcgacaa tgattattgg agatagtgta ccaattcatg ccttgtcagc
     7081 atttagagat gaagatgaat ctattttggt acaaataaaa aaacttttag ccacaataga
     7141 tatagaagaa acctacattt taactgatat tctaggtggt agtgtgaata atgatatgct
     7201 cactattatt aaagagtatg agggattgca attaattact ggaatgaact tacctttagt
     7261 tatttctatt gcaacctata atggaaaaat tggggaaagt gagctagaaa agcttattag
     7321 agagagtcaa caatcagtga ttgattgtag aaaattatta gtagaacaac ttactggagg
     7381 agatgaccta tgattaaatt agtacgtatt gaccatcgtt tgttgcacgg acaggtagta
     7441 ttttcttgga gtaaatcatt aaatatcaat cgtattatta ttgccaataa agaggcagcg
     7501 aatgatgaat tcaaaaaaat gtctttgaat ttatctaaac cgactggcat taaattaaca
     7561 attttttcag ttgaacagtt actagaaaaa atgcctaaaa tcgaaacact taacgaaaat
     7621 atcatgctta tttttggtaa tacaaaagaa attcgtcaat tttgtgaagc ctatcctaaa
     7681 gtacaagaaa tcaactatgg agggataatc aaaaaagaag gttccaagca atttagtaat
     7741 gccattttct tgaatgaatc tgagcaagaa gatgccaaaa aattaaaagc tcttggcatt
     7801 aaacaattta ttcaacaggt accaacttct aaaaaagaag atttgaatac aatgttataa
     7861 aggaggaatg aaaatgctcg ttcagtcaat tggtttaggg ttaatcggtg ttttttgtat
     7921 tttagactca aggcttttag gaagaatgaa ttttgaacga ccgttgattg tcagtacgct
     7981 tgttggtttg gtacttggag acatggaaaa aggattaatt gttggagcct ctttggaatt
     8041 gatgtcctta ggattagtaa atatcggagc tgctgcacca cctgatatga atatggcatc
     8101 aattattgct acagcctttg ccattttatc aaatgcgaat gcagaagcag cattaacgat
     8161 tgccattcca atttcagtgt taggtcaaat actaggtatc ttaatgcgga cgattttgtc
     8221 aaatttgact cacgttgcgg atcgctttat cgaacaagga aaatttagcc aagcaaggag
     8281 aattcatata ctttatggct cgggattata tgctctcatg tattttattc ctatttttct
     8341 agcaatttat tttggaacga atcttgtttc tcagattgtt gatgcgattc ctgagtggct
     8401 aacaaatggc ttaacggttg caagtaaaat tttaccagca tatgggtttg cgttgttatt
     8461 acaaacgatg ttaacgaaaa aaatggtgcc atttttatta cttggatttt taattactgc
     8521 ttattctgga ttgggaatca ttggtattgc agccttcgca tgtattgttg cgtttgtcat
     8581 ggttcaagtt caaggtaaaa actcaaatgg taaccaaaat gagccagatc cattagatga
     8641 cttataagga ggtagattaa gtgaaagaga aagatcaaaa tgcatggaaa tatcggcagt
     8701 tcttatggcg ttcatgggca attcaagcct catggaacta tgaacgacaa atgaacatgg
     8761 gttttatgta cggaattgca ccaattctag ataacattta caagaatcca gaagatgaag
     8821 caatgaagaa agaagcttac gagcggcata tgatgtacta caactgtacc cctcaaacaa
     8881 gtgcatttgt gttagggtta actgcttcta tggaggagca atactacgaa gaccaagaaa
     8941 actttaatcc ggaatcaatc agtgcagtga agacttctct gatgggaccg ctatctggtg
     9001 ttggtgattc tttctttcaa ggaacgattc gggtattagc ctttggacta ggaattaatt
     9061 tagcccaaca aggtagtatt ttaggtccaa ttctagcaat gttaatttcc tttgtacctt
     9121 cctttttagt tacttactat ggtgggaaaa ttggctataa catggggaat aaatacttgt
     9181 ctaaactgta tcaagaaggt ttgatggata aagtaatgta tgtttgctcc attgtggggt
     9241 tgatggtcat tggttcgatg attgccagca tgatcgggat tacaacacca attcaaatgg
     9301 gtaaagcttt tgtcttacaa gatgtattag atagtattat gccacaattt ttaccattag
     9361 gattaacctt cttcatgtac tggttattgc agaaaaaggt caaaacagga tggatgttgg
     9421 caatctgttt agtcggagga attattttta gtttgttagg tatcttacaa taagggaata
     9481 accaataaat ttggttgaat aatataaata aggaggaatt caattgatga attcaaaaga
     9541 aattgttaaa gaaatcgtag cgaaaaaaga ggtaaaatca atcgtatttg ttggctgtgg
     9601 cgcttcaaaa gcggaccttt atcctgcaaa gtattttcta gaccaaaatg cgaagcagtt
     9661 acggattagt cattacacag ccagcgaatt taactatgca acaccagcat ctgtagatga
     9721 gtcaactgtc gttatttcag cttctttagg aggtacgact ccagaaacag ttgaagctaa
     9781 cgcagtagct aaagaaaaag gtgctacagt tattagtcta acacgaagca ttgactcacc
     9841 gttaacaaaa gatgcagatt atgtaattta tcatcgattt gcagagaact atgctgctaa
     9901 attagaaaaa acaggctact gtttagaatt ggcagtagaa cttttacatc aagtagaagg
     9961 ttatgaccat tatgaagcaa tgcaagaagg atttgctact atctatgatc ttgcccaaaa
    10021 aagtgcagaa tcagctcgta aagatgcgaa gaaatttgct gaacgcttta aagatgaaaa
    10081 agttatttat gtaatgagta gtggtgctac acatgaagta gcatactcaa catcaatttg
    10141 cttgatgatg gaaatgcaat ggattaattc tggtaccttc cattcaggag aatttttcca
    10201 cggaccattc gaaattgtgg ataaagacgt accgtttata ttattaatga atgatggaaa
    10261 aactagaaat atagatgctc gagcattaac tttccttgaa cgctttgata cgaaaacaga
    10321 agttgtagat gccttggatt atggcttgtc ttcacatatt gctaaagaag tcttagacta
    10381 ctttaaccca ttcgtgatta ctgcagtatt tagagtttat gcagaagaat tgtcagaagc
    10441 aagagaacat ccattaacta agagacgtta tatgtggaaa ctaacttatt aaaaaataga
    10501 tcaaactaaa taaggtggct tgagaaatta agccaccttg ttttcttcaa tgtgatttta
    10561 tacatatggt aaaataacta gtacatatgg taaaataact agtacatatt gtgaaagcag
    10621 gtggcaagat gcagcgatta aatgactatc tttatcaact aacattgctt ttgaaatcta
    10681 cgccttcaga taattattca gctcgcagta tttcgcaagc tttacagttg aatagaagta
    10741 caatcagtag ctacttaaat gaaggtgttc gtgaagggct tttcataaaa gtaaaaagtt
    10801 acccggtttt atttttacat agaacagctc ttgaagaact acatatcaca ctgaataact
    10861 ccgaaataga atcaattgaa agcttgttaa caatttctca aaaacccgca ttagatcaag
    10921 taattggctc taaaggaagt ttgaaagaag caattgatca aattaaaacg gcagtcttat
    10981 atcctggaaa agggttgcct ttattattga tcggcgctag tggatcaggg aaaacctttc
    11041 tagcgaataa aatatatgaa tatgcagtag aagaaaaggt cattcacaca tcggcacctt
    11101 tcatagatta taattgtgca caatatgtga gcaatccaga gttactatcc tctgctttat
    11161 ttggctacac gaaaggagca tttaccggag caagccagga gcatactggt ttgctagaaa
    11221 aagccgatgg aggagtattg tttttagatg aagtgcatcg cttatctgaa gaagggcaag
    11281 agaaattatt tacatttatg gatacgggcg aattctctcc tatgggggat aatagtattc
    11341 ggaaaaaagc cgatgttcga ttagtctttg caacaacaga aaatatatat acgactttct
    11401 taccaacttt tcttaggaga ttgccagtta ttgttaatct acctagattt caacaacgtc
    11461 cgtcgtttga acgtctgtca ttgattgatg aattttttgt ttctgagagt aaaattttag
    11521 ctaaggaact atctgtttct gatgcattga ttcatttctt aatgaatagt gattttgaag
    11581 gtaacgtggg gaatattaaa aatattatca agtatgcttg cggaaacgca tatatccacc
    11641 agaagagtca gcaaacaatt caagttaaat tattggatat gcctttagag tacagttata
    11701 aattcaagga gcaactaaat aaacccagaa agaaaagagc agatcgaatc tactatccag
    11761 aagaaacacg acaagtgcaa ctagaaagaa acaatcagct ttttgttcaa ttttttgacc
    11821 aattacttac tggattctct gaagtgattg aaaataacaa ggatattaaa ctatttttag
    11881 aagaaatggt taccaaggta acacaagtaa tggatacgct tatttttcaa gaggattacg
    11941 aaaaagagca gtctttatac gccattctaa actatcatat tcgtcaaagt attgatttta
    12001 tgcgagaaag ctacgggttt gaacaagatg gtaaccgggt agtcgctttg gccaactatc
    12061 tatatgtcaa ggataatcaa gtgatttttg aaaatagtct tcaagaa
//
LOCUS       DAYYFU010000072        11667 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000072 DAYYFU010000000
VERSION     DAYYFU010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.076"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..216)
                     /locus_tag="ACHA34_002351"
     CDS             complement(37..216)
                     /locus_tag="ACHA34_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792948.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(232..432)
                     /locus_tag="ACHA34_002352"
     CDS             complement(232..432)
                     /locus_tag="ACHA34_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792949.1"
                     /translation="MNKIIPLLFVLFVGEILMRHGAALHLNFVVFLGILVFCLIFWRF
                     VLTLVSLIFKLIAAIFLIGLFR"
     gene            complement(597..1304)
                     /locus_tag="ACHA34_002353"
     CDS             complement(597..1304)
                     /locus_tag="ACHA34_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792950.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="ACHA34_002354"
     CDS             complement(1297..1734)
                     /locus_tag="ACHA34_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792951.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="ACHA34_002355"
     CDS             complement(1749..2000)
                     /locus_tag="ACHA34_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792952.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="ACHA34_002356"
     CDS             complement(2000..2206)
                     /locus_tag="ACHA34_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFM8792953.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="ACHA34_002357"
     CDS             complement(2643..2903)
                     /locus_tag="ACHA34_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792954.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            3077..3181
                     /locus_tag="ACHA34_002358"
     CDS             3077..3181
                     /locus_tag="ACHA34_002358"
                     /inference="COORDINATES: protein motif:HMM:NF028245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFM8792955.1"
                     /translation="MPVETALGLMISFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="ACHA34_002359"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="ACHA34_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8792956.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="ACHA34_002360"
     CDS             complement(3810..4709)
                     /locus_tag="ACHA34_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFM8792957.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="ACHA34_002361"
     CDS             complement(4722..5315)
                     /locus_tag="ACHA34_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFM8792958.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5391..5540)
                     /locus_tag="ACHA34_002362"
     CDS             complement(5391..5540)
                     /locus_tag="ACHA34_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792959.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5556..7760)
                     /locus_tag="ACHA34_002363"
     CDS             complement(5556..7760)
                     /locus_tag="ACHA34_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFM8792960.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="ACHA34_002364"
     CDS             complement(7771..9231)
                     /locus_tag="ACHA34_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFM8792961.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="ACHA34_002365"
     CDS             complement(9292..11664)
                     /locus_tag="ACHA34_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFM8792962.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2892 a         2432 c         1957 g         4386 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtc
//
LOCUS       DAYYFU010000073        10918 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000073 DAYYFU010000000
VERSION     DAYYFU010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.078"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..281)
                     /locus_tag="ACHA34_002366"
     CDS             complement(<1..281)
                     /locus_tag="ACHA34_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8792963.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEA"
     gene            453..1541
                     /locus_tag="ACHA34_002367"
     CDS             453..1541
                     /locus_tag="ACHA34_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8792964.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1669..3045
                     /locus_tag="ACHA34_002368"
     CDS             1669..3045
                     /locus_tag="ACHA34_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8792965.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3446..5026
                     /locus_tag="ACHA34_002369"
     CDS             3446..5026
                     /locus_tag="ACHA34_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFM8792966.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5224..5447
                     /locus_tag="ACHA34_002370"
                     /pseudo
     CDS             5224..5447
                     /locus_tag="ACHA34_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5652..5972)
                     /locus_tag="ACHA34_002371"
     CDS             complement(5652..5972)
                     /locus_tag="ACHA34_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8792967.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6120..8354)
                     /locus_tag="ACHA34_002372"
     CDS             complement(6120..8354)
                     /locus_tag="ACHA34_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8792968.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8833..9099
                     /locus_tag="ACHA34_002373"
     CDS             8833..9099
                     /locus_tag="ACHA34_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFM8792969.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9099..10826
                     /gene="ptsP"
                     /locus_tag="ACHA34_002374"
     CDS             9099..10826
                     /gene="ptsP"
                     /locus_tag="ACHA34_002374"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFM8792970.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3475 a         1911 c         2215 g         3317 t
ORIGIN      
        1 gcttcttcaa tcaatatttt tcctaaccct tggtgtctgt attcagaaag aatcgccatc
       61 cgctgtaatt tcatttttcc gttttctaaa ggcaaaagac gtacagttcc ttgcggttct
      121 tttttatctg agtacaggac gaagtggata caatgggctt catctttgtc tatttctcga
      181 gacaaaggca caccctgttc gactacaaaa acttgatgcc tgatccgtac agcatctaag
      241 taaatattgc tcattgtatc ttttgtttgt acaactttca tggtctagcc accttccttt
      301 cgtatagtat actggaaaat aaaacaaata aaaagtatgg caagttttca aaaataaaaa
      361 aagaataaaa gaatttttaa cgaatataat atatactagt aagaaagaaa ggaggaaatc
      421 tattgtttga aaaacttaga cagtctaaac taatgttttg gtcgcttgaa ttattgatca
      481 tagctgcatt gatcatgatt tcatcagaaa taaactttat tttcgaaccg atcgggacat
      541 ttttttccac gttatttgca cctgtactga ttgcaggctt tctttactat ttattgaatc
      601 ctattgtcaa gctgttgatg aaagcaaaaa tcaaaagaat atgggctgtc gcaattgtgc
      661 tgctcctttt agttgcagca attatttgga ttttacttag tgtcatccca agtctagtac
      721 agcaaatttc ctcattggca tctaatatgc ctgatttcat caaacaggta gaaagctggt
      781 taaaagaggt agccgaattg cctttgttca aggaagtaga tatcaataaa tattttgagc
      841 aattagacat ttcttacggg acaatcattc agcaattcct tagtggagta tccaatagtt
      901 taggttcgat cgttggaacg atcgcttcgg caaccgtagt aatcattaca gcaccattca
      961 tcttgttcta tatgttgaaa gacggagaaa aactagtgcc aaacatccaa cagtttttcc
     1021 cagaaaaaag aagaaaccaa attgtggagt tattggggca actgaataaa acattggcta
     1081 attacattag cggacaagca atcgaatgtt tattcgttgg taccttcaca tttttaggat
     1141 atatggtaat tggtgtggat tatgctttct tatttggggt cattgcggga ttgacaaacc
     1201 taattccata tttaggacct tatctaggac tagcacctgc atttctagta acagtcttca
     1261 atgatccggt aaaagcatta ctttgttgtg tggttgtgct aatcgtccaa cagttggatg
     1321 ggaatatcat ttatcctaat gtaattggga aatcactgaa aatccatcca ttaacgatca
     1381 ttatcgttct tctagtagct ggaaatattg ccggattgct tggtatcttc ttaggtgttc
     1441 ctttttacgc aatctgcaag acgatcattt cctatataat caaaatcgtc aaagaagaca
     1501 agcaaaatga aaacaagaag aaaattgcta cgacctcgta ggtagtttta gtacacttgt
     1561 ttttattgca aagaaaattg ctttgtgata ccattttaac gtggctatag tgccacgttt
     1621 ttttctatcg taaactaaaa attaatttta taaggagaga atgcgattat gaatgctgac
     1681 cctgagagtc agtcgctgtt agcgcagatt ttattattga tcgttttgac attgatcaat
     1741 gcttttttag cagcttccga aattgctgtt gtttcgatca ataagaatcg aatcgagcaa
     1801 aaagctgatg aaggagatgt gaaatctcaa aagcttttaa aaatattgca gaatccaaat
     1861 aatttcctat cgacaatcca agtaggaatc actttggtca acattttgtc tggtgcttca
     1921 ctagctaata ctttatcgga acggttagct ccggtattag gtggcggcac agcggcaaaa
     1981 aatattgcaa atatcattgt gctggctatt ttgacctatg tatcgattgt ttttggagaa
     2041 ttgtacccta aacgtattgc tttaaataaa tcagaagaag tcgcaaagtt cacttcaggg
     2101 ctgatacgct tgattggtgt agtagctaag cccttcgtct ggctgctttc tgcttccaca
     2161 agtttattgg cgcgtatcac gccgatgact tttgatgatg aagattcaaa aatgaccaga
     2221 gatgaaatgc gttatatgct tgaaaacgaa ggcgtattga ataatgaaga attagaaatg
     2281 ctccaagggg ttttttctct tgatacgaaa gttgcccgag aagtcatggt cccacgaacg
     2341 gatgcattta tgattgatat caacgacact gtggaagaaa atgtgaatga agttttatcc
     2401 gaaaactatt caagaatccc tgtttataac gaagacaaag ataaagtagt cggaatcctt
     2461 catacaaaga atttactaaa agctgcccat aaattcggct ttgataattt agatatcaag
     2521 aaaatcatgc aggagccttt attcgttcct gaaacggtct ttattgatga tctgttatat
     2581 gaaatgaaaa agacgcagaa tcagatggct attttattag atgaatatgg cggcgtcgtt
     2641 gggttagcaa cactagaaga ccttttagaa gaaatcgtcg gagaaatcga tgatgaatca
     2701 gatgaagttg agaatcttta tgaaaaaatc gatgaacacg aatatatcat ccaaggacgt
     2761 atgctgattg atgagttcaa tgaagctttt gacagtgatc tgcatatgag tgatgtagat
     2821 acgatggctg gttatttgat tacagcatta ggcatgattc cggatgaagg cgaaaagtta
     2881 tcgtttgatg tcgataatat tacgctggtt tctgaagaaa tggaaggttc tcgaatcctt
     2941 aaaatccgtg tcatttttca tgatccggaa gaaacagaag cggaacctga agaagaacga
     3001 agatacttca gaaaagaatt cgaagatgac gagccaagaa gataattgga aacttggtta
     3061 tgaaagacgt gttgcttgaa taaacaagca acacgtcttt tcttttagtc agctgacaaa
     3121 aaaatatttg actcttatgg tttggtggct ttttcattcg ctgaatccat gctatactga
     3181 ttcagttgta gtagttgaca aaggttgtga aaaagctatc ctgcattaag taataagagc
     3241 agccaagcaa aaacagcttt tcgtattttt ggcttgggtg ggcttattgc tgataggctg
     3301 tctctagaac gccgtttact cggaaaaaga gactgctaca cggcttttgt cacagcccta
     3361 tatgaaattg tctttcttta agagatcgga caatctgtgc ctagagaaaa tataaataat
     3421 tgaatggaag aaatggagtt aggttatgaa taatccaaaa ttaaagcaac aagtggacaa
     3481 tcgcagaact tttgcgatca tttcccatcc ggatgctggg aaaacaacga ttacagaaca
     3541 attactattg tttggtgggg ctatccgtca agccgggacc gtaaaaggca aaaaaacagg
     3601 taattttgcc aaatcagact ggatggaaat cgagaaacaa cgtgggattt ctgttactag
     3661 ttctgttatg cagtttgatt atcaagggaa acgagtaaat atcttggata caccaggaca
     3721 tgaagatttt tctgaagata cttatcgtac tttgatggcc gtggatagtg cggtcatggt
     3781 aatcgatagt gcaaaaggga tcgaagcaca gacaaagaaa ttattccaag ttgtaaaaaa
     3841 acgtggcatc ccgatattta cttttatcaa caaactagat cgtgatggtc gtgaaccatt
     3901 ggaattgtta gaagaattag aagagctatt agatatcgaa tcttatccga tgaattggcc
     3961 aatcggtatg ggtaaagggc tagaaggtct ttatgatatc taccataatc gtgtagagtt
     4021 ttatcgtccg gaaaattatc aggatgaacg attagtagaa ttggatgaag atggactgct
     4081 tcccgaaaat catcctctaa ctgaaaattc cttatatgaa caagtcttag aagaagtaga
     4141 actaatcaaa gaggctggag atgcatttaa tcaagaaaaa attgctcgtg gcgatcagac
     4201 accagtattt tttggttctg cattgactaa cttcggtgta cagacttttt tagagacttt
     4261 cgttgaattg gcaccagctc cttatggaca caaaacacaa gatgatcaga tggtaagtcc
     4321 ttatgaggaa gaattttcag gtttcgtatt caagatccaa gcaaatatga atccagccca
     4381 tcgtgaccga atcgcttttg tccgaatctg ttctggaaca ttcgaacgag gaatggatgt
     4441 ctggttggaa cgtacaaata aaaaactgaa attaagcaat gtcacacaat ttatggcaga
     4501 ttcgagagaa aacgtcgaaa aagcagtagc aggcgatatc atcggtgtat atgatacagg
     4561 caattatcag attggggata cattgttcga aggaaaacta aaagttgctt atgaagaact
     4621 cccttcattt actccagagc tgtttatgaa agtgactgca aaaaatgtca tgaaacaaaa
     4681 atccttccac aaaggaatct atcaattagt tcaagaagga gcgattcagc tttataaaac
     4741 atatctaacg gaagaatata tcattggagc tgttggtcag ctacaattcg aagtattcca
     4801 atatcgtatg ttaaatgagt ataacgccga agtaatcatg tctccaatgg gacacaaaat
     4861 cgctcgctgg atcgatccag aagatttgga tgagaagatg agttcaagtc gcaatatctt
     4921 ggctcgtgac cgtttcgacc aaccgttgtt cttattcgag aaccaatttg cagaacgctg
     4981 gtttgcggat aaatatccca atgtaaaatt aaaaagtttg atgtaaagaa gtcttgacac
     5041 aagctgccct gcatcaagta ataagaatag ccaaacaaaa acagcctctc atattttgtt
     5101 tagagccgtg acggattttc cgtgacggct ctttttacta tattaaaatc atagaaatgt
     5161 ctaatcaaaa aaatataaaa attattttgc gtacatatga ttaaagaaag gagagtgtga
     5221 cacatggaaa cagaatggca aagacgtgaa agaaaaaaac agaaaaagaa gagatgttac
     5281 gattatgtcg attattagat gtaaaagact gggacaaaaa cgagcaaatt gcacaaaaag
     5341 tacgatcaat catccataaa gaggaaaaag ttaaaaataa agacacgcgt agaactgaaa
     5401 atgaaaagag aaaagtacaa aagaaaagag aaaaatcaaa aagctagaaa gaaaaagagg
     5461 ctgagacaga agcgtttaac tccaagaaat aagctggaat tcacaaaaat ttgaaaagca
     5521 attttcgtga atttcagctt atttccgaag gagttgcttc tgctttcgcc gtttatacgt
     5581 gtttagagcg tgagacaaaa gtgttcttta cttttgtccc acgctcttta attattatgt
     5641 tcaaaatttg tttacacggc taatgtagcg cctaattcat gttttggaat cgagatttca
     5701 acaaggcctg ccaattcgat tactttgatt tcatcctcgt ggtaaacggc gtctttgatt
     5761 ttatctaaga aataattttt agcatcttcg atttctttcg gctggatttt acgtttgtca
     5821 ttgatcagca gaatttcatt atgttcaggg gcttcagtgt agacaatagt ggtatttccg
     5881 gctgtgtaga ccttgactag atgggcatca gtgttttcaa gctggtttaa tacaagacgg
     5941 gaatggctgt ttgttacatt taccaatttc atggcgctca cctcttttat gtatttttaa
     6001 ttgcttgttt ctcttattat aatttctttt ttataaaatg aacagtattt tgacttttct
     6061 gaaaaaagaa tgagacggca tttctcaaag aaataccgtc tcacgctata aaactggatt
     6121 tattcagccg tttcttcggc tgattcttta gccaaacgtt ctggtttaga agtaacgatg
     6181 atttttccat ctttatccag ttttgctttc aactcatgga tagaagggtg atcaagataa
     6241 aattcagcga taccatcttc gatttgttct tggatcgtgc gtcgaagagg acgagcaccc
     6301 atagatggat catagcctaa gtcaactaat ttttctttga cattcgtagg aacttcgatg
     6361 tgaagttgct gtgctgccag catttggttg acatcgtcta gcattagtgt gacgattgtc
     6421 atcaagtttt cttttgataa tgcactgaat tcgatgatcc cgtcaaaacg gttcagaaat
     6481 tctggtgtaa agtagttgtt taattggttc aatacagaac gagtaacacc ttcgcgtgcc
     6541 gcaccaaaac caacgtttgc ttctacttta ccagttccgg cattacttgt cataatgatg
     6601 atcgtatctt taaagctgac cgttcttcct tgtgcatctg tcaatcgacc atcatctaag
     6661 atttgcaaga acatatgcaa aacatctgga tgagcttttt ctacttcatc taacaagaca
     6721 agactgtaag gatttctccg tactttttct gttaattgac cagcttcttc gtatccgaca
     6781 taacctggag gagaaccaat caatttagag acactatgtt tttccatata ttcagacata
     6841 tcgaaacgaa tcattgaatc ttgtgaaccg aatagttcgt aggcaagttg tttcgcaagc
     6901 tctgttttac ctacaccagt tggtccaaca aataagaagg agccgattgg acgattctgt
     6961 ttgtttaatc caacacggtt tcggcgaatc gctttcgcta ctcgatctac agcgttatct
     7021 tgtccgatga catgagcttt caagtcatct gctaagtttt tcagttgtgt ctgttctttt
     7081 tcttttaatt cacccactgg aatacctgtc cgttgttcaa cgattttttc catatctttt
     7141 tcggtaatca caggtgtttc ctcttcagtg agctgacgtt ctttcatttt ttgaagttta
     7201 ttgatctgat cgcgataata agctgctttt tcgaaatctt cttctcgaga agctaaaact
     7261 ttttgttctt ctgcttcttg cagttttttc tcgattgttt taggatcaac gatttggatc
     7321 gtcaagtttt tcttagaacc tgtttcatct agcaaatcga tggctttatc cggcaagaag
     7381 cgatcttgga cgtaacgatt ggataaagtt gcggctgctt cgatggcctc atcagtatat
     7441 tttacatgat gatagtcttc ataacgtttt tgcaaacctt tcaaaatact gatcgtttct
     7501 tcgacagttg gttcatctac ccgtacaggt tgcatacgac gttctaaagc agcgtctttt
     7561 tcgatgatgc ggtactcatt taaggtagta gcaccaacca tttgcaattc tcctcgagct
     7621 aaagctggtt ttaaaatatt accagcatcc atatttccat caccagcaga accagcaccg
     7681 acgatttcgt gaacttcatc tatgaatagg atcacatttt ctgcttgacg gatctcttca
     7741 atcaattttt gcatacgttc ttcaaattgt cctcgtatac cagtaccttg gactaaagat
     7801 acgacatcta aacgaatgac ttctttgtcc attaattttt gcggtacatc accatctacg
     7861 attttttgtg ctaaaccttc aacaactgct gttttaccaa cacctggttc tccaatcaag
     7921 acaggattgt tcttggttcg gcgatttaaa atttcaatga ctcgtttgat ttcgtcatcg
     7981 cgtccgatca caggatcgat atcaccttgt cgtgcttgtt ctgtgatatt gattccgtat
     8041 tctcccaata aaccttctgt attgccttga ggtggctgac caccattgaa gttgttgttt
     8101 ccgccaaatt gtgttggtgg tgtttgctca taatttcctt gctgttgcat ttggcgagac
     8161 atggaacgga aaagatcgtc caaactgcca aatccaaatg gatcattttg ggccatatta
     8221 gtcagacctc cattttgttg atttttgatt ttttgataac agctctgaca gtaatcgagt
     8281 tgtgtccgct gaccattgac agtggcatac aagtgaatcg ttgcctcatt cttgccgcag
     8341 ttttgacaaa gcatgaatca ttcacgtcct ttcacaaaaa tgggtacatc catattttac
     8401 atctcgtttt tcatgaggta aaagaataac ccctagtttt ttaagagatt tatgaggtag
     8461 aaaaggtgaa atctctattg accattcctg acctttgact cttattatac taaaaataaa
     8521 aagggaagca agtaattcgt ttcatatgaa aacaaagagg gtcaaaggaa agaatgataa
     8581 acgtcaggag aagagaaagc gacaagaaaa aggaaataaa cacgtttgtt ataaaatgtg
     8641 aagaaaagga tgaaaaagaa tccggaataa tttgaatact tttatctgaa attgaatgaa
     8701 aactgttgta tctaacgaaa aaaaatgtta atctttacag atgaaagggt ggcatttgtt
     8761 aggattctct gaattactaa aaaagaaaac cctcacataa aaacaacact cacttaaagg
     8821 agaccgatta atatggaaaa gaaagaattt cacgtagtag cagaaactgg gatccacgct
     8881 cgtccagcta cactattagt acaaacagca agcaaattta actctgatgt aaacttagag
     8941 tataaaggta aatcagtaaa cttgaaatca atcatgggcg ttatgtcttt aggtgtaggt
     9001 caaggttctg atgttactat tactgcagaa ggtgctgacg aagctgatgc aatggcagca
     9061 atcgttgaaa caatgaagaa agaaggctta tctgaataat ggttgaaatg ctaaaaggga
     9121 ttgccgctag tgacggtgta gccgttgcaa aagcttacct gctagttcaa ccggatttaa
     9181 cattcagtaa ggcaacagta gaagatactg cggctgaaga agctcgtttg gatgctgcat
     9241 tagctaaatc tactgaagaa ttgcagcaaa ttcgtgaaaa agcagcgcaa agcttaggtg
     9301 aagcagaagc gcaagtattt gatgcccatt tgatggttct ttcagatcca gaaatggtag
     9361 gtcaaatcaa gcaaaatatt aaagacaaca gcgtaaatgc tgaatcagcg ctaaaagaag
     9421 taacggatat gtatatcggt atgttcgaag caatggaaga taatgcttat atgcaagagc
     9481 gtgctgcaga tattcgtgac gttgctaaac gtattttagc gcacttatta ggcgttactt
     9541 taccaaatcc atcaatgatc aacgaagaag tggtcgttgt tgcacatgac ttgactccaa
     9601 gtgatacagc acaattagat cgcaattttg taaaagcgtt tgtaacagac attggtggac
     9661 gtacttcaca ttctgctatc atggcacgtt ctttagaaat tccagcaatc gttgggacaa
     9721 aagaaattac tgctaaagtc aaagaaggcg atattttggc tgtcaacggg atcgaaggcg
     9781 atgtaatcat cgatcctaca gatgaacaaa aagctgaatt tgaaaaagca ggtgcagatt
     9841 acgcagcaca aaaagcagaa tgggaaaaat taaaaaatgc agaaactgtc acagctgacg
     9901 gcaagcattt tgaattagct gcaaacatcg gaacaccaaa agatctagtt ggtgtccata
     9961 ataatggtgg agaagcagtt ggtctgtacc gtactgaatt cctgtatatg gattctccag
    10021 acttcccaac agaagatgac caatatgaag cttacaaagc tgtattagaa ggtatggaag
    10081 gaaaacctgt agttgttcgt acaatggaca tcggtggaga taaagaacta ccatatctgc
    10141 aattacctca tgaaatgaat ccattcttag gatatcgtgc attgcgtatc agtttatctg
    10201 aacaaggcga cgaaatgttc cgtacacaaa tgcgtgcatt gcttcgtgca tctgttcatg
    10261 gtaatttacg tatcatgttc cctatggtag ctacactcaa agaattccgc gcagcaaaag
    10321 cgatctttga agaagaaaaa caaaaattga taagtgaagg caaggaagta tctgatacga
    10381 tccaagtcgg catcatgatc gagattccag cagcagcagt cttagcagac aagtttgcaa
    10441 aagaagtaga tttcttctct gtaggtacga acgatttgat tcaatacaca atggcagcag
    10501 accgtatgaa cgaacgcgtt tcttacttgt accaaccata taacccatca attttacgtt
    10561 tgatcaaaaa tgtaattgat gcagcacacg ctgaaggaaa atgggcaggt atgtgtggag
    10621 aaatggctgg agatcaaaca gctgttccat tgctagtagg tatgggtcta gatgagttct
    10681 caatgagtgc gacatctatt cttaaaacgc gtagtttaat gaaacgcttg gacacagcga
    10741 aaatggctga acttgctgat cgtgcattga aagaatgcga tatgatggaa gaagtcgttg
    10801 aacttgttca tgaatatgta aaataaacaa cgtatcaaaa gctggtgtgg cttgtaaaag
    10861 agctacgcca gtttttttgt tcttttttaa gaaaacgttt ttgtcagtaa gaatagac
//
LOCUS       DAYYFU010000074        10754 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000074 DAYYFU010000000
VERSION     DAYYFU010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.079"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..693
                     /locus_tag="ACHA34_002375"
     CDS             <1..693
                     /locus_tag="ACHA34_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792971.1"
                     /translation="YLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYFVYCPELLLYD
                     VKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKV
                     NSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISF
                     SPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENW
                     AELRKLVEYGII"
     gene            680..2101
                     /locus_tag="ACHA34_002376"
     CDS             680..2101
                     /locus_tag="ACHA34_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8792972.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            2114..3121
                     /locus_tag="ACHA34_002377"
     CDS             2114..3121
                     /locus_tag="ACHA34_002377"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFM8792973.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            3133..4137
                     /gene="neuB"
                     /locus_tag="ACHA34_002378"
     CDS             3133..4137
                     /gene="neuB"
                     /locus_tag="ACHA34_002378"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFM8792974.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            4134..5282
                     /gene="neuC"
                     /locus_tag="ACHA34_002379"
     CDS             4134..5282
                     /gene="neuC"
                     /locus_tag="ACHA34_002379"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFM8792975.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            5255..5932
                     /locus_tag="ACHA34_002380"
     CDS             5255..5932
                     /locus_tag="ACHA34_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM8792976.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            5883..7064
                     /locus_tag="ACHA34_002381"
     CDS             5883..7064
                     /locus_tag="ACHA34_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM8792977.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            7077..8054
                     /locus_tag="ACHA34_002382"
     CDS             7077..8054
                     /locus_tag="ACHA34_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8792978.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            8054..8962
                     /locus_tag="ACHA34_002383"
     CDS             8054..8962
                     /locus_tag="ACHA34_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HFM8792979.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            8963..9715
                     /locus_tag="ACHA34_002384"
     CDS             8963..9715
                     /locus_tag="ACHA34_002384"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8792980.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            9720..10667
                     /locus_tag="ACHA34_002385"
     CDS             9720..10667
                     /locus_tag="ACHA34_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM8792981.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         3901 a         1356 c         1978 g         3519 t
ORIGIN      
        1 tatttgaaga agagtatatt ttcaacaagt aaaaacgttc aaagagtttt taaaatatta
       61 aataaaaata gcatcttcaa aaaaaaaatt gacgattatt ttgtctattg tccggaattg
      121 ttactttatg atgtaaaaaa tataaaaaaa attcccaaaa tagatgaaaa cagtaaattg
      181 atgaaaaaca tacaatcaat ctttgggata gaagaagttt ttgtcggaga taaatgtatc
      241 ttttttgatc aacctttatt atctgatggt tatggcatag atgaaacaga tattttcgct
      301 aaagtaaata gtatattttc tggaaaaaaa gaaactttaa ttaagcttca tcctagagca
      361 aggacaaatc gttatggtga aaaaaacata ttgaattcca gtttaccgtt tgaagtcttg
      421 ctacttgaaa atgacttctc ttcaacactt ttgattagtc ctatatctac aatttctttt
      481 tcaccatcta tgatgtttga aaagacgatc aactctatta tgttggctaa attaatttta
      541 aatgaatata cagcattatc tgaagataaa aaagaaacat tgacaaatat tgttcttttc
      601 tgtgaacatt ataaccgtct atcagaagaa aaaattttac tcccagaaaa ctgggcagaa
      661 ttaagaaagt tggtagagta tgggattatt taacacactt tccaagtcaa aattagttaa
      721 gtccagtttc tggtatacta tagggaattt ttttattaag ggaatttcat tcataactat
      781 tcctattttt gtaaatatta ctactcaagc agaatatggt ttgattaata atttcacttc
      841 atttgtatca attttttcga ttcttgttgg gctaagccta aattcggcaa ttaataatgc
      901 aagttttgac tataaagatg acataaatga atttatgtca tcggtacttt ctctagctac
      961 actttcgtta attgtgttct tattaatagg gaacatatat tttttgtttt caaatgcttt
     1021 ttttgatatg aaccaacttg tatttaattt gatgttatta caaagttttg ctagttttgt
     1081 aattagttat atccagtctt attttacgat taactttaac cattttaaat atttgatttt
     1141 atcttttata tctacttttt tgaatattgc aatctctgta gctttgatgc agacattata
     1201 ttttgatgat aaatatgttg gcagagtagt cggcggaaca ctgtcatttg tgttgatagg
     1261 tttagtaatt tatttaagaa taatgataaa gggtgctcat ttttttgata aaaaacagtg
     1321 gagatacgct ttaaaaatct ctttgccgct aatcccacat tccttagcaa atatagtttt
     1381 agcacaattc gatagaatta tgattaattc ttatttaggt cctgctgcgg ggggaatata
     1441 ttcctacatt agtaacatag gtattattct aagcgtaata tggatgtcta ctaataacgc
     1501 ctgggttcca tggttctatg gtgagatgag taaaaataat gaaaaaaaag tgaagaaagt
     1561 tagtaactat tatttagtag tctttacttt gattacgatg attgtaatgg tagtttcgat
     1621 tgatttagca aaatttatgg ctccgaaaga ctattattct gggttatctt tagtggttcc
     1681 gataacctta ggctatttct ttcagttttt atatagttta ccagttaatg ctgaatttta
     1741 tgagaaaaaa acatcgttta ttgcgattgg tacagtagca agtgctatag ttaatatact
     1801 attaaacatg attttcatac ctaggttagg aataattgct gctggataca caaccgtatt
     1861 tacgtatttt ttacttttta tttttcatta taatatcgct aaaaaaatta cgaataaaca
     1921 attatttgac acaaaaaaaa tatgtatcat aataattttt gtatcaattt tttctttgat
     1981 acttcatatt ttaatcaatc aaatgttgat tcgttacgtt ttgacatcat tattgataat
     2041 tagtttagtt atatattttt taaagattaa gaatcgaatt atagatgact ataattcata
     2101 ggaggagata taagtgttta aaggaaaaac aatattagta actggcggaa ctggttccat
     2161 tggaagtgaa attgttcgtc agcttttaaa atatgaacca aaagctatca gaatttatag
     2221 tagaggtgaa gaaaaacaat tttatatgca acaagagctc tcaaatcaca ctaatttgag
     2281 atttttaatt ggtgatatca gagactctca aaggattgat tatgcatgtc gtgatgtgga
     2341 tattattttt catgctgccg caatgaaaca cgttccagca agtgaatata atcctatgga
     2401 agctgtaaaa acaaatgtga tgggaactca aaatgtaata gatgcagcga tcaaaaataa
     2461 tgtaaaacat tttgtaggta tcagtacaga taaagtagtg agtcctacta acaccatggg
     2521 tgcgacaaag ttattgagtg aaaaattaat aatagctgcc aatcagtata aaggaaaaac
     2581 agaaacaata ttttcttgtg taaggtttgg aaatgtcatg gggtcaagtg gatctgttat
     2641 tcctcttttt agtaaacaaa taaaaaacaa taaagatatt actttgacag atgttgatat
     2701 gacacgattt atgatgtcaa taccggatgc tgcaagatta gttctgtctg ctgctatgaa
     2761 ttcaaaattt ggagaaacat atgtcttaaa aatgccggca atcaatattt taactttagc
     2821 tcaatcatta ataaaaatat ataatgatac acatgatgaa aaatatagag gtgaaataaa
     2881 agttgtaggt atcaggccag gagaaaaatt atatgaagag ctaatgacta cagacgaaat
     2941 tgaacgctca tatgaaaatg ataaaatgta tatcattcca ccacactatc atgctactga
     3001 cgaactgtac aaagattatg aaaaaattaa aatcactgca tactcttcag atgcaacaga
     3061 attaatgagt ggacaggaat ttattagaat cgcaaaagag aataatgtcg tgacttggta
     3121 aggagtttta tgatgaaatc agaagtatat gtaattgcag aaattggtgt aaaccataat
     3181 ggaagtctag atttagcttt agaaagtatt gataaagcta aagaatgtgg agctaacgcg
     3241 gttaaatttc aaacatttaa aacgaataag ctagtttcca aaaaggctcc aatggcaaaa
     3301 tatcagaagg ataatcttaa agaggacagt tctcaatttg aaatgctaaa aaaattagaa
     3361 ttatctcaat ctgattttat tagaataaaa gattattgta tacaaaataa aattgatttt
     3421 ttgtctactc cttttgatga agaaagtgca gactttctaa aacaaattgg tgtacaagga
     3481 tttaaaattg gctcaggaga tttaacaaat ttaccttttc ttagaaaaat agatatgtat
     3541 gggttgccag ttttattatc tacaggtatg gctgatattc atgaggtcag tgaggctgta
     3601 gattgtttca tagatagtcc agttactatt ttacattgta cttcaaatta tccggctagt
     3661 tctgaagata ttaatttatt ggcgattaac acattaaaag aaatgttcgg gatacccgtt
     3721 gggtactccg atcattcatt gggatatgat gttggtattt gtgctgttgc gatgggtgct
     3781 aaggtaattg agaaacattt taccttggat aaggaactac ctggtccaga tcataaagct
     3841 tctttagatc ctcaggaatt tgcagatttt gtaaagcata ttagaaacac tgaaatattt
     3901 ttaggtgatg gtgttaagcg agcaatgcct tctgaactta gtacaaaaga agtatcaaga
     3961 aaaagcctag tggtcactga gaatttaaat gtgggagata ttcttacaga gaaatctctt
     4021 acaataaaac gtcctggaaa tggaatagct cctaagtttt tatatcaata tattggcaaa
     4081 aaagttaaaa gaccaataag tgcagatagt gttctgcttg aggaagatgt tgaatgaaaa
     4141 aaatacttat agtaagtgga acacgagctg attatggaat atataaacct gttgctgaga
     4201 atttactaca agataattgg gatgtatcat ttgttgtttc tggaatgcat ttatcccata
     4261 aatatggatt tactaaagaa ttgatcttag ctgacggatt tagaattagt ggagaagttc
     4321 aaagtttgtt tttagagaac aataagggaa acatggctcg gtctgtggca ttagagataa
     4381 atggtttcac aaatatattt gaacaaacta gccctgattt tgtattatta ctaggggata
     4441 gaggagaaat gttggcagct gcgatagctt gtttgtatct tggaatcaaa acagcacata
     4501 ttcacggagg cgaagtgtct ggtactgtag atgaatcaat aagacatgcc ataacgaaaa
     4561 tagcatctgt tcattttgct gctacggaaa aaagcaaaga gcgtttaata aaaatggggg
     4621 aagattcttg gagagttttt cccgtcggag ctcctagaat agatactatt ttaaataata
     4681 gattacctag ttttagagat ataaaagaaa aatatcatct aaaatttgaa aagaaaaaat
     4741 acgttttgca agtatttcat ccagttgtaa ccgaactaga aaatatagaa aaacaaattt
     4801 tagctttgat caattcaatg gaacaacaat ctcaaccaat tatttgcatt ttacctaata
     4861 gtgatgctgg tagcgaaatt atcagaaaaa tgtaccagaa atatttaaaa acggatatta
     4921 tttatattac gaatttagag ccggaggaat acctgactat attaaaagag tgcaaattta
     4981 tgatcggaaa ttcttcttct gggataattg aagctgcatc attcaaaata ccagtaataa
     5041 atttaggaac cagacaaaat ggacgagaac aatctaaaaa tattataaat gcaaatctaa
     5101 atgaaaatga aattgaaact gctttgaata aaatacaaga tcctcagttt ataaaaagct
     5161 tgaaaaatgt taagaacatc tacggtgacg gcaacagttc tagaagaata acaaaaacat
     5221 tgaatcaaat attgaatgat ggggaagata ttaagtggat tcaaaaacaa atagcttatt
     5281 aaaatatact agtgatccat acattattat tggggctggc ggacacgcaa aagtcattat
     5341 tgaaacctta agattagaaa ataagttaat atatggattt tttgatgata agcaaaataa
     5401 aatgcaagat ctgaaatatc ttgggaaaat aaacgaattg aatagctggg cggatagcaa
     5461 aaaaaaaatc tataaattta ttgtagctat tggtgactgt gtagtaagaa aaaagattat
     5521 aaaaaaattg aatatgccaa gtgagcaata tggaatagct gttcatccaa gtgctatagt
     5581 ggcaaaagaa atttcaattg gttatggaac tgttattttt gcgaatgctg taattaaccc
     5641 agatgctgta attggagagc atgctataat aaacacaggt agcattgttg agcatgattg
     5701 tagaattaat aactatgttc atttatctcc aggtgtatgc ttagcgggtg gagtacatgt
     5761 aggtgtagga actcaaatag gaattggttc tcaatgtatc cagatgaaga aaattggatc
     5821 ttggtgtatg ataggcgcag ggagcactat tgttaaggat attccctcac attccttagc
     5881 ttatggcaat ccagctaaaa ttaagaaaga aggaattaat tttgaattct agaattttat
     5941 tatcaagtcc tcatatggga ggaacagaag aaaaatatgt tgctaaggct tttaaagata
     6001 attggatagc gccattaggt gaaaatgtgg ataaatttga agaaagtatt acaaaatata
     6061 ctggttctaa gggcggagct tctgcgttaa gttcaggtac agctgccata catcttgcct
     6121 taagactgct taatgttgga aaaaacgata ttgtctttgc tccttcattt acttttatag
     6181 ctacagtaaa tcctatttta tatcaagggg ctgtaccagt gttcattgac tcagaaccta
     6241 atacatggaa tatgagccct gttgcactag agagcgcatt agaagaagct gaaagaaaca
     6301 accaattacc taaggcaatc atcgtagtga atttatatgg tcaaatggct gattatgaac
     6361 gaattttagc tattgcaaac caatttaatg ttccagtgat agaagatgca gcggaatcgt
     6421 taggttctac ttataaggga atttcgtcag gcttatttgg gacaattggg atctattcat
     6481 ttaacggtaa taagatcatc acaacctcag gtggaggagc tctagtgtct agtgataaaa
     6541 aatatgttga gcattcaaga tttttagcaa cacaagcaag agatcaagca ccgtattatc
     6601 aacatagcga aataggttat aattaccgtc taagcaatat ttctgccggt atcggacgtg
     6661 gacaaatgga agtattgaat gatagagtag ctaaacgaag agaaattttc aatcgatata
     6721 aggaagcttt ttctggaatt gatagaattg aaatgatgcc agaagtggat ggcatggtgc
     6781 caaatcgttg gttgtctact ttaacgatca atgattataa ttctattgat caagtagtta
     6841 acgtaataaa taaactaaat gaaaaaaaca ttgaaacaag agctttgtgg aaaccactac
     6901 atttgcaacc agtatttcaa ggggtaaaaa tgtattacca tgataataat cttagtgtgt
     6961 cagaaaagtt atttaatagc ggcttatgct taccttctgg aagtaatatg acagatagtg
     7021 aacaagaatt tgttattgat gagtttaaaa aaatattcaa gtaggtgtga tattttatga
     7081 acataggaat tattggttta ggttccatgg ggaaaagaag acttagactc attcaagaac
     7141 actttaatga tattttacta tgtggtgttg atactaatga ggcgagacga aaggaagttg
     7201 aaaaggaatt tggaatacca acttataata gtctaacaga ggcaaaaaat ttgtttgaaa
     7261 taaattcagt ttttgtttgt acatccccta taacccatga atttattata aaagaggctt
     7321 taaatttaga ttgtaatatc tttacggaaa tcaatttatt aaatgaatat tatgatgaaa
     7381 taatcgaagt agctaaaaag aaagatagat tactttatct atcttctaca tttttgaaga
     7441 gaaaagaaat tcaatatatt aagtctaaag tgacgcataa taaaaaaatt tcttatcaat
     7501 accatgtagg acagtattta cccgactggc atccttggga agattatcgc aatttttttg
     7561 tttcaaacaa aaaaactaat gggtgtagag aaatattcgc tattgaatta ccttggatta
     7621 tagatacatt tgggaaagtt aaatcgttta ctgtaatgac ccagaaatta tccaatcttg
     7681 atattgatta ttcggacagt ttcactctta ttattgagca tgaaactggt atagttggaa
     7741 ctttaagtgt agatgttgtt tcaagagtag ctaaaagaaa tttaactata attggtgaaa
     7801 agattttaat agaatgggaa ggtaacccgg attctctatt ttcatggaat gaagaaactt
     7861 ctagtatgac agatattcaa ttgtacgatg aggttatgca tgataataat tattcacata
     7921 ctattattga agatgcatat ttagaggaga ttaaagaatt tttcacgctt ttggaaaaga
     7981 aaatggaacc ccaatatact tttgaaaaag ataagtatat tattaactta attaacgaca
     8041 ttgaggtaaa ttaatgatga aagtaatttt ttttgggtta gggtcaattg gaaagagaca
     8101 tctaaaaaat ctaataagct atggaaaaaa gaattctatt aattttgata taacagctta
     8161 tagtagcagt ggaaaaaaag agtttgaaga aataacgtat attcaaaata gagacgattt
     8221 ggatacacat tatgatatag cttttattac aaatccgact tttaagcatc tggaaacact
     8281 aaatgtaatt gttgaaaaag ttgattacat atttttagaa aaacctgctt tttctgagag
     8341 tattgatctt actcaatata attatgatta caaaaatatt tatgttgctg ctccactgag
     8401 gtataaaaat atcatggaag ttgttcgtaa tagtattcaa agtaaaaaag tatattctgt
     8461 aagagctatt tgttctactt atcttcctga ttggagaaaa ggtactgatt atagaaataa
     8521 ttattctgcc tttaaatcta tggggggagg agtagaatta gactgtatac atgaattgga
     8581 ttatattata aacctatttg gtttccctaa atcagtaaaa aaacagtttg gtaaatattc
     8641 gaatcttgaa attaacagta atgatttagc aacatatata ttgagatacg atgataaaat
     8701 tatagaaatt catttagatt attttggaag agttcctaca agaaaatttc aattgtttac
     8761 tttagatgaa tgtatcaatg ttgacttatt aaataataaa ataagttttg aagaaaacat
     8821 tgttaaagaa atgcctgaat ctgctaatga tatgtatgag aaggagttga aatatttctt
     8881 tgaaaacgta gtaacaggca aagaaaactg gaatgattta tatcatgcaa acgaagtatt
     8941 gaaaatagct gaggagaaat aaatggaaat tttatttact atttgtggta gagctgggtc
     9001 taaggggttt aaaaataaaa atttgaaata tttggcagga aagcccctag tccatttcgc
     9061 tttagcaatg atagatatct atcaacaaaa acatcctgaa cataattgca cgacttgttt
     9121 gaatacggac agtaatgagt tagtagaatt gacgactaag ttaaatccgt ctgtgaaaat
     9181 tgtagaacgt aaacctgaat tagctacaga tactgtaggg aaaattgaag tgatacgtga
     9241 ttcattctta caactagaaa gagaagataa tatatttgat ttaattatag atttagattt
     9301 gacttcacct ttacgtgaag tttctaatct tgaagaatta atagatgaac atctaaaaaa
     9361 tttagataaa gacgtctact tttcagttgt ttctgcaaga cgtaatccct attttaatat
     9421 ggtaaaaaga attgaaaatg aagttactat agttaataaa tcaagataca catctaggca
     9481 acagacacct gaagtttttg aaatgaatgc ctcaatgtat tcttttaaac ctgaatttct
     9541 aaaaaaaagt aattatattt ttgatgggca atgtgggata ataaaaatga gagattatct
     9601 aattttagat attgatagtg aagaagattt taaatggtta gaatttttgt ttcctaaatt
     9661 tttagaagat tcttcggagc ttcaagatat ctataattat ttatatcgga ggtaaaagga
     9721 tgagctgtta tcttatcacc ggtggggctg gcttcattgg ttctacctta gccaatcatt
     9781 taggagaaga acatgtggta atcgtcgtcg atgacttatc aatgggaaaa gtagaaaatc
     9841 ttgatatgac gaaaaatatc acttttattg agggagatgt cgctgagaaa tccttgatgg
     9901 aagagatcat gaaagctaat aaatttgatt atatctttca tttagcagca gttgctagtg
     9961 tggcagattc agttgcccga ccagtagaaa cacatcgtgt gaattttgaa agtgtattaa
    10021 tgattttaga attgatccgc aagtatcaac ctgatttaaa acggatcgtg tttagttctt
    10081 cagcggctgt ttatggggat gaaccgactt taccaaaaaa agaagagtcc gtgattcgcc
    10141 cattgacgcc ttatgcggtt gataaattcg ctgcagaaca atatgtgcta gattattgtc
    10201 atttatatga tgtacccggt agcgcagttc gttttttcaa tgtctatggt ccaaatcaaa
    10261 atccgaattc tccatacagt ggtgtgattt ctattctcgt agatcgctat aagaaacaaa
    10321 tcgtaggaga agcaacttca tttacattat ttggtgatgg aagccagtct agagactttg
    10381 tttatataga tgatgtaatc caagcattac ttttagttgc gaatgaggaa gctgcactgg
    10441 ggaaacaatt caatgttgga acgggtaaag caaccacact tttggaattg attcatgcaa
    10501 tcaatgaaat tctcggtgta gaattgacac ttgaatacaa agaagagcgt tcgggtgata
    10561 tccatgactc tctagcagat attactaaga tccagtcaat tggctatcaa ccgaaatatg
    10621 gtattttaag tggaatgact aattatttga aaactgaaat caattaaaac atactaagat
    10681 aagattagag ggtaaattca ggctctttgt caataaggac tgatgagttt tgtaaaatca
    10741 aatctgggat agaa
//
LOCUS       DAYYFU010000075        10723 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000075 DAYYFU010000000
VERSION     DAYYFU010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.080"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(241..1089)
                     /locus_tag="ACHA34_002386"
     CDS             complement(241..1089)
                     /locus_tag="ACHA34_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8792982.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1253..2155
                     /gene="gnd"
                     /locus_tag="ACHA34_002387"
     CDS             1253..2155
                     /gene="gnd"
                     /locus_tag="ACHA34_002387"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFM8792983.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2157..3158
                     /locus_tag="ACHA34_002388"
     CDS             2157..3158
                     /locus_tag="ACHA34_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFM8792984.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3155..3439
                     /locus_tag="ACHA34_002389"
     CDS             3155..3439
                     /locus_tag="ACHA34_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFM8792985.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3472..4269
                     /locus_tag="ACHA34_002390"
     CDS             3472..4269
                     /locus_tag="ACHA34_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8792986.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4283..5116
                     /locus_tag="ACHA34_002391"
     CDS             4283..5116
                     /locus_tag="ACHA34_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8792987.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGGFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5134..5991
                     /locus_tag="ACHA34_002392"
     CDS             5134..5991
                     /locus_tag="ACHA34_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8792988.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6013..6522
                     /locus_tag="ACHA34_002393"
     CDS             6013..6522
                     /locus_tag="ACHA34_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8792989.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6559..6960
                     /locus_tag="ACHA34_002394"
     CDS             6559..6960
                     /locus_tag="ACHA34_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792990.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7072..7425
                     /locus_tag="ACHA34_002395"
     CDS             7072..7425
                     /locus_tag="ACHA34_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792991.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7459..7902
                     /locus_tag="ACHA34_002396"
     CDS             7459..7902
                     /locus_tag="ACHA34_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8792992.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7919..9004
                     /gene="uxuA"
                     /locus_tag="ACHA34_002397"
     CDS             7919..9004
                     /gene="uxuA"
                     /locus_tag="ACHA34_002397"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8792993.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8997..9710
                     /locus_tag="ACHA34_002398"
     CDS             8997..9710
                     /locus_tag="ACHA34_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8792994.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9917..10066)
                     /locus_tag="ACHA34_002399"
     CDS             complement(9917..10066)
                     /locus_tag="ACHA34_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792995.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10462..10662
                     /locus_tag="ACHA34_002400"
     CDS             10462..10662
                     /locus_tag="ACHA34_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8792996.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3588 a         1694 c         2320 g         3121 t
ORIGIN      
        1 aaaaataata aaaaaaagaa aagataaatt ttttactact gtatactaac tttgttatcg
       61 catctaacaa gtaactcgaa taataccaat acaaccttct ccaaagatgc gatgacatac
      121 gttctaaccc accgactatt gatgttctgt accacccttg cagaaccaaa aaagccacta
      181 ccaaatattt ggtagtggct ttttttgttt tatggatttt tcgctttttc tgttatggtg
      241 ctattctttg atttcagaaa tagtatgtcg aactttagcg atagaacgac gtgattcttt
      301 ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa atgcttagtt gagaaatacg
      361 ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa atagaaataa aaacgacatc
      421 gccaagttta gcaagggaag aattagcttg gctagtaata acgattgttt ttgctccagt
      481 ttttcttacg gtttcagcaa tatggattgt ttcttttgat tgccctgtgt gagaaatcaa
      541 aacagcacag tctttttccg tcaataaaga agcttccatc aattgaatat ggtaatccgt
      601 gctgtgagaa gttgcgatag gggaacgcaa aaatttatga taagcgtcag ttgctacaat
      661 ctctgaaccg ccaacaccga aaaaatagac acgtttagat tcattgataa tagtagccgc
      721 tttttttaga tccttttgtt gtaacaactt ttttgtgtca tttaaagttg aaatactaga
      781 ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt ttctgaatat tttcgtggat
      841 agaaatagca gatagatcat tttcttggat caacatggcc attttaaaat ctttaaatcc
      901 attataacca agtttttttg taaattgaaa aaaagtagag tcagcaatgc ccagttcact
      961 tgctaaattg ctaataggac tatgacttac tttgcttgga ttttctaaaa tataatcggc
     1021 gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt ttgatttgta gtcttatgga
     1081 gacgctcatg agaattcctc attttcataa taagattatc attttttccg ttctcattaa
     1141 tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa aatatttttg taaaatctat
     1201 ttacaaaaaa atttttttgt ataatgaggg tataagaaat ggaggcgcta tcatggaaat
     1261 tggtgtaatt ggtttgggaa aaatgggatt gaatttagct ttaaacttaa aagagcacca
     1321 tcacaaagta caaggactcg acatttcaag tacagctgtt agtcaggcaa gagaaaatga
     1381 cattcctgct tatcaagaga tctctgaatt tctagctact tttgagcaga aaaaaatcat
     1441 ctggttaatg cttcctgctg gagagataac cgaaaacatg ttaagaaaac tttttccaaa
     1501 actcggacca ggagacatta ttattgaagg cggaaactct tattataaag attcaatccg
     1561 tcgagcagaa gagtttcaga aaaatgaaat cggttacttg gattgtggga cttctggagg
     1621 aatcgaagga gcacgacatc aagcctgctt aatgattgga ggagataaag aaacttttct
     1681 atccgtggaa caattattca aagatgtagc aattgaaaga ggatatctgt atgctggtgc
     1741 atccggaagc ggacacttct tgaaaatgat ccataatgga atcgaatatg gaatgatgca
     1801 agcaatcggt gaaggattcc aacttgtaca aaaaagtgag tatgcttttg acttggaaca
     1861 agtcgcacgt gtttggaatc atggttcggt gattcgcggc tggctgatgg aaatcatgga
     1921 agatcagtta aacgaacatc ccaatttaag taattatcgg ggaatcgttt ctgcatcagg
     1981 tgaagcaaaa tggacggttg aaacagcgct agaaatggct gttccagttc cagtaattgc
     2041 aactagtttg ttcatgagga atctttcaca ggaagaagat agtttttcag caaaagtagt
     2101 ttcagcacta agaaatgggt ttggcggaca tgaaatagtt gaaaagggtg agtaaaatgc
     2161 cagattatca tatagcaatc gttaattcca gcagtttcgg aagagtcttt ccacaccact
     2221 tggaaagatt aaagtcagtt ggagacgttc atttctttac tgttgatcaa gagattccag
     2281 gaaaagaact agcggagtta cttaaaggat tcaatatcat tattgcaagt gttacgccat
     2341 tttttcgacg agattttttt gaaaacaaag atgaactgct attgatttcc cgacatggta
     2401 ttggttacaa caatattgat ttaaaagcag caaaagaaca ccagacaatc gtatctatca
     2461 tacctgcttt agttgaacga aatgctgtag cagaaaataa tataactaat ttgttgtcca
     2521 tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga cgatcgatgg gaagatcggg
     2581 cggagttcgt cggtaggacg ctgtttaaca aaacagttgg tgtgattggt gcaggaaata
     2641 ccggaagcgg agtaatcgaa attttgcgta acggttttcg ctgtgatgtt ctagcttatg
     2701 atccttacaa atcagattta tacatcgaaa gctttggtgc aaaaaaagta gaacttcaag
     2761 aattactgga acaatcagat atcatttgtt tatgtgcgaa tttgacagaa gagaactatc
     2821 acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa tgtttatcta tctaatagtg
     2881 caagaggcgc tctagttgat gaagaagcga tgattaatgg tctgaaaagc ggaaagatcg
     2941 caggatatgc cacagatgtt ttagaagaag aaccgggacg tcaagatcat ccgtatttag
     3001 cgtttgaaca agtagtgatt acgccacata tatcggccta cacaagggaa tgtttagaag
     3061 caatgggtga aaaatgtgtc agagatgtcg aacagatcag tcagcacctt cttcctgaac
     3121 gttctgtcca gacaaagagc aactatataa aaggatgatg aagaaaatgg tgaacggttt
     3181 aaaagtgaaa acaggccctc aattttattt atatgaagaa ggtggtattt caaaagtttc
     3241 tgatctcttg aaatcatacg gcgcgaaaag agtactggtc acccacggaa cggtatcatg
     3301 ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa acgattcaat ttttttatca
     3361 tcgttattct ggagaatgta gttatgctga agccagacga atagctacaa tcatcaaaaa
     3421 aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac tgaccgatct agtgggttat
     3481 agtgccaatc ttgcaagaat cgatttcgga gtcatcccta ctttagctag taattgtgct
     3541 ccatggacac ctcttgctgt catgtataag gagaatggac aagcagaagg gaaaacagaa
     3601 cacttttaca gacaagctgc tttttttata acagatcccc aattagtgat tgattcccct
     3661 gttccatatt tcattgccgg gttagcagat acactagcaa aatggtatga atctgagctc
     3721 atcttagaac aagatttttt gcaagatgaa tcatttttga agctagcaca agatactgcg
     3781 aagatctgca aagaagagat tttgaatcat tctgctaaag caatccaaga tatggcacaa
     3841 agaaaactga catcggagtt caaacagctt tcagagatta tctttgctgt tgctggttta
     3901 gttggaggat taggagataa atacgcaaga aatgcggcag cacatgctat gcatgacgct
     3961 atcagtaaat atattccgga aagccatcgt tttctacatg gagaaaaagt agcgtatggg
     4021 attttctatc aactggcact agaaggtcgc tgggaaaaaa tagacgagtt atatcctttg
     4081 tatcgggaat tagaactgcc ggtttctcta gaacaaatgg gattatttcc gaaagataaa
     4141 cagataatag ataaaatggc tgaatttatt gattcgaaag agaaagtgca tttgattcct
     4201 atagaggtaa cacagaaatc actgatacag tcaatgatgg aactggaagg ttatatcaga
     4261 aagatctgaa ggaggaggac acatggaaac attaagtgta gtacaaagtt tgttgattgc
     4321 tttatgggta gccgcggtta tgtcccgctg gttaggtgga ggagccacgt tgaccttacg
     4381 tttttctcct ttgatgactg gattagtcgt cgggttgatc atgggggaag ttgcacaagc
     4441 gatgattgtt acagccgctt tgcaaatgat ttatatgggg ggtttttctc caggaggatc
     4501 aatgccagct gaaccatcta ttgcagcagc tatcgcagta ccagtcgcat tattagggga
     4561 tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc ggattgctcg gaagttactt
     4621 gtatcagttt cgttttttca tcaatacttt tttaggtaaa tatacagaca aagctgtaga
     4681 agatttgaat acaaaagcta tggcccgatc aattattctt tatccaacgg ttgcctcatt
     4741 tttattattc gttccgcttg ttttcattgc tttatattta ggggcgccat tgatcgctga
     4801 tgtaatcaaa gctttagagg gaacagtcgt tatccacatt cttgaagtag taggtggagg
     4861 gttagcggca atcggtatcg cgacaactgt atacgttatc ggtcgaaaag attatctggt
     4921 ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa tctttagata ttacgatggt
     4981 tacttatgcc atttttggga ttattatcgc tttgatcttt gtccagtcgc aaaaagggaa
     5041 acaagtacag cctgccacaa gcaataacaa ttcagcatct tttgatgacg aagatgacta
     5101 tgatgacgga ttttaattta ggaggaatgg gaaatggctg aaataatgga taatcaaaca
     5161 ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag cctatctgag atggcatttt
     5221 gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc catcactact atacggaatg
     5281 atgccaatct tgaaaaaact ttataaagat gaagactctt taaaagcagc atataaacgg
     5341 cagttactgt tcttcaacac tcaattaagc tggggtggcg gtgtgattac tggcctaatg
     5401 tcttctatgg aacagcaaag agcagaagaa gagtatgaag aaaaagagat cttgatgcag
     5461 gatgacttga tgtataacac gaaagcggga ttgatggggg cactagcagg aatcggagat
     5521 gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa tcgcggtacc ttgggcacaa
     5581 cagggaagtc ctttaggcgc actttttcct tttatctgct ttgggttgta tcaggtttta
     5641 ctaggggtat ttttcgcaag acaagctttc actatgggcc gtaatgctac aggcctaatg
     5701 caaaacactg gtgttcagac agcgattgaa atgctatcag ttttagggtt gtttatgatg
     5761 ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat tgaaatttgc gatatccgga
     5821 cgtgagtttg ttatccagga aattcttgat caaatcgtgc cagggatatt acctttagct
     5881 gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat tgaaagtcac acaagcatta
     5941 ttatggctga ctggaatatt aattgtgcta gcaggaatcg gtattttata aaaagaaggg
     6001 aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg atgagcgttt gatccatggg
     6061 caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt ccatttttgt tgtagacgaa
     6121 gttgtagcaa aagataaatt tatgagagat ctttacaaaa atgcaggaag ccgtacagga
     6181 caaaaaacaa ttgtcattac accagagaaa gctaaattct attgggatga atatcaattt
     6241 aaagaataca attgtatttt gatcgcaaaa acagtccaaa cgatttacga cttagtaaaa
     6301 cacggaatcc ccatggaaga attgaatatt ggagggattg ctcaaaaaaa tcctgataca
     6361 gatcttttag tgacgaaatc tgtttattta aataaatcag atgctgaaaa attgaaagaa
     6421 atgaatgaac aatatggcgt aaagaatatt tattttcaag ccactccatc tgcacctaaa
     6481 acaggattaa aagatgtatt gaacaagttt gatttgtcat aaacaaatgc ttagaaaaaa
     6541 gataaagagg gcgaaaaaat gaagaagaac gagctgtttc gtgattggga atttcgttat
     6601 cgctatattt accgtaagcg ccgtacaaaa aagagtaaac agcgttttct ttcagcatta
     6661 gtatcagata tttacagtat gagaacagat gtgacagtca ttgcttacga tacgctagcc
     6721 taccgatcaa aaaatattta tgttggagat atcgaaaaag cagagaaagt tatctgtact
     6781 tattacgata cacccgttca tgcattagga agttatttca tgtttgattg gaaagaccaa
     6841 aggaaaaaaa caatctactc tatattattg tccttcattt tgttgttcag tttaggttgg
     6901 tggggaatga tgatatataa caaaaatcct catcacgtat tcgatctgct atccgtataa
     6961 acaggtatca ctgtactagc atttgggagc tactttttct tattgggaaa agcagctcgc
     7021 ggatggccta gcagacaaac ttttattcga aatacttcat ccatattaac gatgctagaa
     7081 atgatccgta cgattgatga tccgagtgta gcgtatgcgt ttgttgatga aggctgttat
     7141 ggagaaaagg gcttagacag cgtgagatcg ggtatgaaaa aagaggctat tcttttttat
     7201 ctagactctg ttggggcaga tacaccgcta cagttttcgg gaaattattt ttcaaataaa
     7261 gaacagtggc tgaaacaagt tgataagttg aaagaaaaaa acgtcaatta tatttttagt
     7321 gcaagaaaaa agcaagcgca gttcttctat ttgaccaaaa cagatttacg agggaaaaca
     7381 tttaattggc aaaatgccaa tcagattatt gctctttttc gataagtaaa aagtgcaaaa
     7441 caaggagtga gaagactaat gttggaaatc atgatcgtta cacatggtaa gttgagcgaa
     7501 ggactgaaag atgcggcgga agtaattgtc gggacatcga gccatattga aacaatgtgc
     7561 ttagagtctg gggaggacat ccaaacattc ggagagaagg tcaaagaaaa attaactatg
     7621 atcaacacag cagaacccat gctagtattt gttgatctaa ttagcgcaag tccgtacaat
     7681 caagcgctct tggcagtcaa tgaactgact gaagaaaaga aagcagccac ttatgtcata
     7741 gggggcgtga atctgccaat gttgttagaa gcaatcaatc atcagatgct tcacacaccg
     7801 atagaagaag cggtggaaca aattgtcaaa caaggaaaag ataatttaac tgtttggcat
     7861 gcttcagcgg aagaaaaaaa attagatgag gacgattttt agaaggaagg ctgaaaagat
     7921 gaagtgggga tttagatggt atggtgaaaa aggggattcg atcccattaa aatatgttcg
     7981 ccaaataccg ggaataaatg ggataattgg aactttgctg actaaaatgc ctggagatgt
     8041 ttgggagatt tcagaaattg aggagttgaa agcatctgtt gagagccaag gactggaact
     8101 gcttggaatt gaaagtgtag cgatccacga tgcaatcaag gcagggacag atgaacgaga
     8161 ttactatatt gataactata tacgaacaat caaaaacttg tctcgatgtg gggttcgatt
     8221 gatttgttat agcttcaaac caatttttgg atgggcgaaa actgatttgt ttaatgaaaa
     8281 caacgatgga agctattctc ttttatacga tcagaaaatc gtcgatcaga tggagccagg
     8341 agaaatgtat caattgatac acagccagtc aaagggattc aaattgccgg gttgggaaga
     8401 agaacggttg aaaaaattcg ctcagctgga aaaactgtat gaaggaatga ccgaagaaga
     8461 attatttgaa aatctgactt attttttaga aaaagtgatt ccagtgtgcg aaaaagcaga
     8521 tgtaaaaatg gctatccatc cagatgatcc gccatgggaa ctttttggtt ttcctagagt
     8581 gacgaaaaat cttggcgact tgaagaaaat tctaaatgca gtagattctc catataatgg
     8641 actaacgctg tgcacaggat ctttaggggc aaatccggaa aacgatttag ttgaaatcat
     8701 ccatgaggta ggttcgcgca tccattttgt ccacttcaga aacgtggagt ttttaggaga
     8761 aagaaagttc aaagaaacgg cacatccctc tacagaaggc tcccttgata tgtatcatat
     8821 tatgaaagcg ttggtgcaag aagggttcga cggtcctata cgccctgatc atggtagaac
     8881 cgtgtggggt gaagtggcta tgcctggata tggcctgtat gatcgtgcaa tgggactcac
     8941 ttacatgcaa ggtctatatg aggcgataag taaggaaaaa ccactgggag agcattatga
     9001 ataaaatcaa gctcttcatc tttgatatgg atggcctttt attagaaact ggacgtttag
     9061 cttatcgtgc ttatgtaaaa tcagcacaaa agtatgatta tgaaatgcgt aaggaagtct
     9121 attatttact aactggccaa acagaaatgg cgataagaga acaaatgggc attttgtacg
     9181 gagaagatgt tccatatatc aaatggcgag aagcaatcaa tcaatataaa gaaaaaatcg
     9241 tgaaagaaga taagagagta tatacgaaaa aaggagcaga agaaatttta tcttttgcaa
     9301 aagaaagagg aatccataca atcgtggctt cgtctaatac acgggtaaaa gtagaaatgt
     9361 atttaaaaat ggaaaatcta tatgccttgt ttgatcaaat catttcaggg gatgatgtaa
     9421 aaaaaggaaa gccggaacca gagatctttt tgaaagcttg ttcaaaaatg aatattcctc
     9481 caagtgaagc acttgtattt gaagactcga ttgcagggat cgaagcagca aggcgagcgg
     9541 gcattttgtc ttttctcatc aaagatcatt tgatagggtt gcccgatcat aatgggaagc
     9601 ataagttgaa agtagatgta agtttatatg aatccaataa agcagactat gaattttatg
     9661 acctgcatca agcaaaacga tttttagaaa gtaaagggtt gtatctctaa taaagaggac
     9721 tattttctag tcctctttat ttattgtcct gcacgactga gggaacagat gaaaaagaag
     9781 taatttttta ttttgaagta tctaccttat ttgtagtgaa caatttagaa caaacacaaa
     9841 acgcatctac tgttatctac cagtagatgc gtttaaagag aagaacgaga atcgtccata
     9901 tgagggtttc cactccttat gcagattttt ttaattcttg cactgcttgt tcgatcgtaa
     9961 cacctgtcgc tacatgattg gtattttctt tgtcaaaaac gacaaataga ttcaaatctg
    10021 tatccaaggt tactcgatat tgtatatctt tgttatagaa tgtcatagcc attctccttt
    10081 ctttaaatac ttgctgaaag gcaacgaacg cgttgataac gaaattccat ttataagtat
    10141 accaccaatt gaaaggtttt catagttaag aaattttaaa gtcaaaagtt gcagataaaa
    10201 gcaaataaac aagaaaacaa aaagtgaatc aatcgcggtt attcaacttt ttcttcgatt
    10261 cgaagacaat agaagacaat gaattttata tttcattgca ttatccttgt gttattagaa
    10321 aaagcatgat atattaaatg agtaatctat tttgaaacgt aatttgagcg atatatctac
    10381 atagataaaa ctccttttgc ctatcaaata tttattgcaa cgaaaaaacg tacattgata
    10441 cgtatcagga ggaatttatt tatgaataac ggtacagtaa aatggttcaa cgcagacaaa
    10501 ggttttggct ttatcactgg tgaagatgga aatgatgtat ttgcacattt ttcagctatc
    10561 caaggtgacg gcttcaaaac tttagaagaa ggccaagcag tgtcttacga tgttgaagat
    10621 ggtcaacgtg gccctcaagc aacaaacatt gttaaagctt aataaaaatt gacttttaac
    10681 cgtcttttcg aaagaaaaga cggttttttt gtacgaaaaa att
//
LOCUS       DAYYFU010000076        10532 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000076 DAYYFU010000000
VERSION     DAYYFU010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.081"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..162)
                     /locus_tag="ACHA34_002401"
     CDS             complement(25..162)
                     /locus_tag="ACHA34_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM8792997.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /gene="mobT"
                     /locus_tag="ACHA34_002402"
     CDS             complement(159..1343)
                     /gene="mobT"
                     /locus_tag="ACHA34_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8792998.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="ACHA34_002403"
     CDS             complement(1599..1853)
                     /locus_tag="ACHA34_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8792999.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="ACHA34_002404"
     CDS             complement(1878..3020)
                     /locus_tag="ACHA34_002404"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFM8793000.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="ACHA34_002405"
     CDS             complement(3080..4432)
                     /locus_tag="ACHA34_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8793001.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="ACHA34_002406"
     CDS             complement(4503..4847)
                     /locus_tag="ACHA34_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM8793002.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="ACHA34_002407"
     CDS             complement(4857..5231)
                     /locus_tag="ACHA34_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8793003.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="ACHA34_002408"
     CDS             complement(5244..5558)
                     /locus_tag="ACHA34_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8793004.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="ACHA34_002409"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="ACHA34_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="ACHA34_002410"
     CDS             complement(8976..9638)
                     /locus_tag="ACHA34_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793005.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="ACHA34_002411"
     CDS             10255..10485
                     /locus_tag="ACHA34_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793006.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT         2895 a         2344 c         1786 g         3507 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ct
//
LOCUS       DAYYFU010000077        10193 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000077 DAYYFU010000000
VERSION     DAYYFU010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.082"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..626
                     /locus_tag="ACHA34_002412"
     CDS             120..626
                     /locus_tag="ACHA34_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8793007.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            643..1542
                     /locus_tag="ACHA34_002413"
     CDS             643..1542
                     /locus_tag="ACHA34_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFM8793008.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1629..2513
                     /locus_tag="ACHA34_002414"
     CDS             1629..2513
                     /locus_tag="ACHA34_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8793009.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2705..2776)
                     /locus_tag="ACHA34_002415"
     tRNA            complement(2705..2776)
                     /locus_tag="ACHA34_002415"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2741..2743),aa:Arg,seq:ccg)
     gene            2836..4170
                     /gene="rpoN"
                     /locus_tag="ACHA34_002416"
     CDS             2836..4170
                     /gene="rpoN"
                     /locus_tag="ACHA34_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFM8793010.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4319..5410
                     /locus_tag="ACHA34_002417"
     CDS             4319..5410
                     /locus_tag="ACHA34_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFM8793011.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5451..6452
                     /gene="gap"
                     /locus_tag="ACHA34_002418"
     CDS             5451..6452
                     /gene="gap"
                     /locus_tag="ACHA34_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8793012.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6562..7752
                     /gene="pgk"
                     /locus_tag="ACHA34_002419"
     CDS             6562..7752
                     /gene="pgk"
                     /locus_tag="ACHA34_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFM8793013.1"
                     /translation="MAKKTVKDIALKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7856..8611
                     /gene="tpiA"
                     /locus_tag="ACHA34_002420"
     CDS             7856..8611
                     /gene="tpiA"
                     /locus_tag="ACHA34_002420"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFM8793014.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8727..10025
                     /gene="eno"
                     /locus_tag="ACHA34_002421"
     CDS             8727..10025
                     /gene="eno"
                     /locus_tag="ACHA34_002421"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFM8793015.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3564 a         1887 c         2112 g         2630 t
ORIGIN      
        1 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
       61 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
      121 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
      181 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
      241 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
      301 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
      361 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
      421 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
      481 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
      541 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
      601 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
      661 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
      721 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
      781 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
      841 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
      901 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
      961 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
     1021 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
     1081 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
     1141 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
     1201 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
     1261 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
     1321 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
     1381 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
     1441 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
     1501 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
     1561 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
     1621 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
     1681 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
     1741 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
     1801 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
     1861 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
     1921 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
     1981 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
     2041 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
     2101 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
     2161 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
     2221 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
     2281 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
     2341 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
     2401 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
     2461 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
     2521 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
     2581 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat tttctttggc
     2641 ggacagtttt cacttaaaaa taaagaagca actattcgta aattatgcta aaatagcagt
     2701 agaacgcgtc ctgagggaat cgaacccccg tctcaagaac cggaatctta cgtgatatcc
     2761 actacactaa ggacgcaagc actgggataa ttgtaactaa acagataaaa aattacaagg
     2821 ggattggaca aaaaaatgaa atttgagcaa aatttttcac aaaaacagca gcagacacag
     2881 aaactagcca tgacgcaaca gttgcaacag tccatccaag ttttacaata taacagtgag
     2941 gagctgttgg cattcgttga gaatcaagca atggaaaatc cattggtgga agttgttgag
     3001 ccggaatggc agccagatta cataaaagct tcttcatctt cttatgaagg agaagagaca
     3061 aattacctaa atcagattcc tgatacaaaa gggtctttat ttgattcatt gatcgaacaa
     3121 gtccatttga attaccgaga tacttttttg aggaaaatcg ttctttatct tgtagaatac
     3181 atcgatttga acggattttt gacgattagt ttagaagaag ccggaaaaga gattggagct
     3241 acgcctatcc aaatgctcga tgcgttgact ctgattcagc aattagaacc agctggagta
     3301 ggtgcacgta gtttgcaaga atgtctcatg cttcaaacag aacgagatga ttatgcgccg
     3361 gaattagctt atatcgtgtt ggaagaatgt tttgaggaac tggtggagcg aaaatggaaa
     3421 gaaatcgcac aacgtttttc tgttgatcta catgctgttc agcagatatt cgactattta
     3481 caaacgttga gtccaagccc tggccggatt ttcgaccgta gcagcgaatt gtacattatt
     3541 cctgatgtcc gggtattagt ggacgacgat aaaaatgtac aagttatctc aaaccgaaaa
     3601 aatcagccaa atatacgctt tcaagagagt tacttcaaac aaatgtcaca acaagcagat
     3661 aaagaaactg aaaattattt aaaagaacgg aaacaggaat tcgaatggtt gaagaaaacg
     3721 atcctacaac gtggggatac aattttgcgg gttgctcaag tcattgtttc ccggcaaaaa
     3781 gctttcttta tcgacaagga acgtccaatc aagcctctta cgttaaagga agttgcaact
     3841 gagatagatg tgcatgaatc cacggtcagt cgtgcagtca acgggaaata tttggaaaca
     3901 gacttcggtg tatttgaatt aaaaaaattt ttcactacac gaattcctac gaatagtaca
     3961 gaacagacag aagacctttc agcagataca gcgaagaaaa agctgcagga attagtcgat
     4021 caagaagaca aaaacaagcc gttgtctgac cagaaattgg ttgaattgct gaaaaaagat
     4081 gagatagcga tctcaagacg aacagtagca aaatatcgtg atttattagg aatcccgagt
     4141 tcatcaaaaa gaaaacgtta tgataactaa tcagtcaaaa gactagaaaa agtatttcac
     4201 tttttctagt ctttttagtt gaaaaacgag agcaaaactg ttagacttac agtcgtgagg
     4261 ttgagatttc cctttattga tgtgaaattc gaatagttaa tttctttttt tatcgtatat
     4321 gggtcgtttt aagtctacat tggacgtaaa gtgtccaact aaggagatcg ctatgctgga
     4381 tgaaataaaa atgattgaat ctgttgcacc agatattatc gaagtgttgc aagaacgcta
     4441 taaaatttta cgaaatatct attggatgca gccgattgga cgtcgaagtt tatcggaaag
     4501 tatggggatc acagaacgag tattgcgaac agaaacagat cttttgaaaa atctgaattt
     4561 gatcgacacc tcaaaaagcg gtatgacgct gacaactaag ggcgaagaag tttatcaaag
     4621 ccttgaaaac ttcatggatc agcttttagg tacgcatcaa accgaacaac agttggcaga
     4681 atattttggt aaccaacgct gtatcgttgt ttcaggaaac agcgaagagc agaccaaagt
     4741 ggctgatgca ttcggcgaag cgctgagcga agccctagac cgactacttc cagaaggcga
     4801 gaatatcatc gctgtgatgg gaggaactac tatggctgtg gtagccgaac aattgtctaa
     4861 tctggaaaat aagaaacggc ataacctgtt tgtccctgca agaggcggga ttggtgaagc
     4921 gataactgta caggcaaatt ctgtcagtgc aagaatggct gctaaagcaa acggtaacca
     4981 ccgtgcactt tatgttccgg aacaattaag tctggcaact tacaattctt tactcaatga
     5041 gccatctatc caagaagtct taaacttgat tagtgaagcg aattgtgtta ttcatagtat
     5101 tgggcgtgca ttacacatgg cagcacgtcg taagatgact gaaaaagaat tagtcatgct
     5161 gaaacaagca aatgctgttg cggaatcttt tggctacttt ttcaatgaaa aaggtgaagt
     5221 cgtttataag gtccctagaa tcggcataga attgcaagat ttggaaaaag tcccgatcat
     5281 catggcaatc gcgggcggaa aatcaaaagc caaagcaatt cgagcttata tgaaaaatgc
     5341 accaaaacaa acgtggctca tcactgatga agctgctgca aatgagattt taaaaggggt
     5401 aaccctttaa aataaatatt tttttgattt tcataaggag gaaatcttaa atgacagtta
     5461 aagtaggtat taatggtttt ggacgtattg gacgtctagc attccgtcgt atccaagatg
     5521 tagatggaat cgaagtagta gcaatcaacg acttgacaga tgctaaaatg ttggcacact
     5581 tgttaaaata cgacacaact caaggacgtt tcaacggaac agttgaagtt catgaaggtt
     5641 cattcaacgt aaacggaaaa gaagttaaag tattagctaa ccgcaaccca gaagaattac
     5701 catggggcga actaggcgta gatatcgttc tagaatgtac tggtttcttc acttctaaat
     5761 cagcagctga aaaacattta acagctggtg ctaaacgcgt tgttatctct gctcctggcg
     5821 gaaacgatgt tccaacaatc gtttataaca caaaccacga aacattaact ggtaaagaaa
     5881 cagttatctc aggtgcttca tgtactacta actgtttagc tcctatggct aaaacattga
     5941 acgacaaatt tggtgttgtt gaaggattaa tgacaactat ccacgcttac acaggtgacc
     6001 aaatgactct agacggacct catcctaaag gtgacttccg ccgtgcacgc gctgctgctg
     6061 caaacatcgt tcctaactca acaggtgctg ctaaagctat cggtttagta attcctgaat
     6121 tgaacggtaa attagacgga gctgctcaac gtgttcctgt accaacaggt tcattaacag
     6181 aattagtaac agttcttgaa aaagaagtaa ctgttgacga aatcaatgca gcaatgaaag
     6241 aagcttcaaa cgaatcttac ggatacaaca cagacgaaat cgtttcttca gatatcgttg
     6301 gtatgactta cggttcatta tttgatgcta cacaaactaa agtaatgaca gttggcgaca
     6361 aacaattagt taaaactgtt gcttggtacg acaacgaaat gtcatacact gcacaattag
     6421 tacgtacttt agaatacttc gctaacttat aagatagaca aacgtacaag tgtaaattat
     6481 agacatgtac atttaaaagc ggggaagcaa tcgcgcttct tccgcttttt ttgataataa
     6541 gtttattcag gaggaaataa aatggctaag aaaacagtga aagatatcgc tttaaaagac
     6601 aaaaaagttc ttgtccgtgt tgacttcaac gttcctttga aagacggcgt gatcactgac
     6661 gacactcgta tcaaagcagc attgccaaca atcaactacg ttctagaaca aggcggaaaa
     6721 gcaatccttt tctctcacct tggacgtgta aaaacagaag aagataaaga aggaaaatct
     6781 ttagctccag ttgcaaaacg tctaggcgaa ttattaggta aagaagtaac attcgtacct
     6841 gaaactcgcg gagaacaatt agaagaagca atccgcaaca tgaatgacgg cgacgttgtc
     6901 gtattcgaaa atacacgatt tgaagatatt gacggtaaaa aagaaagcaa aaatgataca
     6961 gaactaggta aatactgggc ttcattaggc gatgtatttg ttaacgatgc tttcggtaca
     7021 gctcaccgtg cacatgcttc taacgtaggt atcgcttcaa ctggtatccc aacagttgct
     7081 ggattcttga tggaaaaaga aatcaagttc atcggtgaag cagtagaaga accaaaacga
     7141 ccaatgattg caatccttgg tggcgcaaaa gtttctgata aaatcggcgt aatcgaaaac
     7201 ttgattccta aagcagataa aatcttgatc ggtggcggaa tgacttacac attctacgaa
     7261 gcaaaaggaa tcaagatcgg taactctcta gtagaagctg ataaagtgga actagcaaaa
     7321 gaattaatcg aaaaagcagg cgacaaactt gtcttgccaa tcgacaatgt ctgtgcacca
     7381 gaattttcaa atgatgttga aacacaagta atcgaaggag acattcctga tggtttgatg
     7441 gctttagaca tcggcccagc atcagtaaaa ttatttgctg atacattaca aggtgcaaaa
     7501 acagttgttt ggaacggacc tatgggtgta tttgaaatga gtaactttgc taaaggtaca
     7561 atcggtgttt gtgaagcaat cgctaactta gaaggcgcaa caacaatcat cggtggcgga
     7621 gactcagccg cagcagcaga acaattagga tttgctgaca aattcacaca catctcaaca
     7681 ggtggcggcg caagcttgga attgcttgaa ggtaaagaat taccaggact agcagcaatc
     7741 aacgataaat aaacgaccaa gtttgctcaa ttctgacgag taatcgatca aaaggtgaag
     7801 ggagttttca tgactcccta gccatttttc aataaattaa ggagtgtgtt cctcaatgcg
     7861 taaacctatt atcgcaggta actggaaaat gaacaaaact gcatcagaag caaaagaatt
     7921 tgctgaagca gtaaaaaaca aaatcccttc aaatgacaaa gttgattcag tcatcggatc
     7981 tcctgcatta ttcttacaag aattaatgac atcagctgaa ggtacagaac taaaaatctc
     8041 agcacaaaac tgctactggg aaaactcagg tgcgttcact ggtgaaactt caccagcagc
     8101 tcttgctgat ctaggtgttc attatgtgat catcggacat tcagaacgtc gtgaatattt
     8161 ccatgaaaca gacgaagaca tcaataaaaa agcgaaagct atctttgcaa atggtatgac
     8221 accaatccta tgctgtggcg aatctttaga aacctatgaa gcaggcaaaa ctgctgaatg
     8281 gatcgaagga caaattacag ctggtttagc tgatctttca gctgaacaag tgtcaaacat
     8341 ggttattgct tatgaaccaa tctgggcaat cggtactggt aaatcagctg acgcacaaat
     8401 cgctgacgaa atttgtggag ttgtacgtca aacagttgaa aaactttatg gaaaagaagt
     8461 atcagaagca gttcgtatcc aatacggcgg ttctgtaaaa ccagaaaata ttgctgaata
     8521 catggcaaaa gaaaatgttg acggtgcttt agtcggtggc gcaagcttgc aagcagattc
     8581 attcttgtca ttgttagacg ctgtaaaata attgttgaaa atttgcgaaa aatgcaagaa
     8641 atgtttgcac taatgagcaa aattaactaa aatcgtatat gagggaaatc ccttaggaaa
     8701 aaacaaactc aaaggagaga caaaacatgt caattattac tgatgtttac gctcgcgaga
     8761 tcttagactc acgtggtaac ccaacaatcg aagtagaagt atacacagaa agcggagctt
     8821 ttggccgcgg aatggttcct tcaggtgctt caactggtga atacgaagca gttgaattac
     8881 gtgacggaga caaagctcgt tatggcggta aaggtgtaac taaagctgtc gacaacgtaa
     8941 acaacatcat tgctgaagca atcattggct acgatgttcg tgaccaaatg gctatcgaca
     9001 aagctatgat cgctttagac ggaactccta acaaaggtaa attaggtgcg aacgctattt
     9061 taggtgtttc tatcgctgtt gcacgtgcag ctgctgacta cctagaagta cctttatacc
     9121 actacctagg cggattcaac acaaaagtat tgccaacacc aatgatgaac atcatcaacg
     9181 gcggatcaca tgctgataac tcaatcgact tccaagaatt catgatcatg cctgttggcg
     9241 ctccaacatt caaagaagct ctacgtatgg gtgctgaagt attccacgca ttagcttcaa
     9301 tcttgaaagc tcgcggatta gcaacttctg ttggtgacga aggtggattt gctccaaacc
     9361 ttggttcaaa cgaagaaggt tttgaagtaa tcatcgaagc aatcgaaaaa gctggctatg
     9421 tacctggtaa agacgttgtt cttgctatgg atgctgcttc ttcagaattc tacgacaaag
     9481 aaaaaggtgt ttacgtacta gctgattcag gcgaaggcga aaaaacaact gacgaaatga
     9541 tcaaattcta cgaagaatta gtttctaaat atccaatcat ctcaatcgaa gacggattag
     9601 acgaaaacga ctgggacgga ttcaagaaat taactgacgt attaggcgac aaagttcaat
     9661 tagttggtga cgacttgttc gtaacaaaca ctcaaaaatt atctgaaggt atcgaaaaag
     9721 gaattgctaa ctcaatcctt atcaaagtga accaaatcgg tacattaaca gaaactttcg
     9781 aagctatcga aatggctaaa gaagctggct acacagcagt tgtatctcac cgttctggtg
     9841 aaacagaaga ttcaacaatc tctgatatcg ctgttgcaac aaacgctggc caaatcaaaa
     9901 ctggttctct atcacgtact gaccgtattg ctaaatacaa ccaattacta cgtatcgaag
     9961 accaacttgg tgaagttgca gaatacaaag gtttgaaatc tttctacaac ttaaaaaaca
    10021 aataattctt ttctaatgaa gatacatgat cgactattga aagatagtcg agtatgatca
    10081 cttagttaag taatcgaact ctctacttgc tgaaatatcg gctctttgtc aataaggact
    10141 gatgagttgt gcaaaatcaa atctgagtca gaatgaacca cattctggct cag
//
LOCUS       DAYYFU010000078        10129 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000078 DAYYFU010000000
VERSION     DAYYFU010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.083"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..309
                     /locus_tag="ACHA34_002422"
     CDS             136..309
                     /locus_tag="ACHA34_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793016.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            949..1422
                     /locus_tag="ACHA34_002423"
     CDS             949..1422
                     /locus_tag="ACHA34_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFM8793017.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="ACHA34_002424"
     CDS             1431..1658
                     /locus_tag="ACHA34_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8793018.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="ACHA34_002425"
     CDS             2294..2665
                     /locus_tag="ACHA34_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793019.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="ACHA34_002426"
     CDS             complement(2921..3163)
                     /locus_tag="ACHA34_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8793020.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="ACHA34_002427"
     CDS             complement(3195..4097)
                     /locus_tag="ACHA34_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8793021.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="ACHA34_002428"
     CDS             complement(4110..4298)
                     /locus_tag="ACHA34_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8793022.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="ACHA34_002429"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="ACHA34_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8793023.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="ACHA34_002430"
     CDS             complement(4913..5806)
                     /locus_tag="ACHA34_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8793024.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6807)
                     /locus_tag="ACHA34_002431"
     CDS             complement(5884..6807)
                     /locus_tag="ACHA34_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8793025.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6856..7206)
                     /locus_tag="ACHA34_002432"
     CDS             complement(6856..7206)
                     /locus_tag="ACHA34_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8793026.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="ACHA34_002433"
     CDS             complement(7239..8141)
                     /locus_tag="ACHA34_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8793027.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="ACHA34_002434"
     CDS             complement(8134..8991)
                     /locus_tag="ACHA34_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8793028.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9325..>10129
                     /locus_tag="ACHA34_002435"
     CDS             9325..>10129
                     /locus_tag="ACHA34_002435"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8793029.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNF"
BASE COUNT         2913 a         1897 c         1690 g         3629 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca tctttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     9181 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     9241 caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat
     9301 gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg
     9361 cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa
     9421 aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt
     9481 tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg
     9541 aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga
     9601 aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa
     9661 gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat
     9721 ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt
     9781 agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga
     9841 gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg
     9901 cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa
     9961 tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc
    10021 gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta
    10081 acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttct
//
LOCUS       DAYYFU010000079        10086 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000079 DAYYFU010000000
VERSION     DAYYFU010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.084"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(247..759)
                     /locus_tag="ACHA34_002436"
     CDS             complement(247..759)
                     /locus_tag="ACHA34_002436"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFM8793030.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="ACHA34_002437"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="ACHA34_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFM8793031.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3655)
                     /locus_tag="ACHA34_002438"
     CDS             complement(3212..3655)
                     /locus_tag="ACHA34_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFM8793032.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="ACHA34_002439"
     CDS             complement(3656..4444)
                     /locus_tag="ACHA34_002439"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8793033.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /gene="rnz"
                     /locus_tag="ACHA34_002440"
     CDS             complement(4441..5382)
                     /gene="rnz"
                     /locus_tag="ACHA34_002440"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFM8793034.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="ACHA34_002441"
     CDS             complement(5559..6146)
                     /locus_tag="ACHA34_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFM8793035.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="ACHA34_002442"
     CDS             complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="ACHA34_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFM8793036.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="ACHA34_002443"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="ACHA34_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8793037.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="ACHA34_002444"
     CDS             complement(9063..9998)
                     /locus_tag="ACHA34_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8793038.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DAYYFU010000080        10085 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000080 DAYYFU010000000
VERSION     DAYYFU010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.085"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            163..393
                     /locus_tag="ACHA34_002445"
     CDS             163..393
                     /locus_tag="ACHA34_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFM8793039.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            490..1791
                     /gene="murA"
                     /locus_tag="ACHA34_002446"
     CDS             490..1791
                     /gene="murA"
                     /locus_tag="ACHA34_002446"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8793040.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1804..1980
                     /locus_tag="ACHA34_002447"
     CDS             1804..1980
                     /locus_tag="ACHA34_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8793041.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2115..2411
                     /gene="yidD"
                     /locus_tag="ACHA34_002448"
     CDS             2115..2411
                     /gene="yidD"
                     /locus_tag="ACHA34_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFM8793042.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2475..2942
                     /locus_tag="ACHA34_002449"
     CDS             2475..2942
                     /locus_tag="ACHA34_002449"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8793043.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3027..3911
                     /locus_tag="ACHA34_002450"
     CDS             3027..3911
                     /locus_tag="ACHA34_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8793044.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4025..4261
                     /locus_tag="ACHA34_002451"
     CDS             4025..4261
                     /locus_tag="ACHA34_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFM8793045.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4349..4429
                     /locus_tag="ACHA34_002452"
     tRNA            4349..4429
                     /locus_tag="ACHA34_002452"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4383..4385,aa:Tyr,seq:gta)
     gene            4436..4507
                     /locus_tag="ACHA34_002453"
     tRNA            4436..4507
                     /locus_tag="ACHA34_002453"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4468..4470,aa:Gln,seq:ttg)
     gene            4632..4802
                     /locus_tag="ACHA34_002454"
     CDS             4632..4802
                     /locus_tag="ACHA34_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793046.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5022..5729
                     /locus_tag="ACHA34_002455"
     CDS             5022..5729
                     /locus_tag="ACHA34_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8793047.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5789..6433)
                     /locus_tag="ACHA34_002456"
     CDS             complement(5789..6433)
                     /locus_tag="ACHA34_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFM8793048.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6560..6847
                     /locus_tag="ACHA34_002457"
     CDS             6560..6847
                     /locus_tag="ACHA34_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793049.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6955..7275
                     /locus_tag="ACHA34_002458"
     CDS             6955..7275
                     /locus_tag="ACHA34_002458"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8793050.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7356..8669
                     /locus_tag="ACHA34_002459"
     CDS             7356..8669
                     /locus_tag="ACHA34_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM8793051.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8666..9385
                     /locus_tag="ACHA34_002460"
     CDS             8666..9385
                     /locus_tag="ACHA34_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFM8793052.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9513..10064
                     /gene="raiA"
                     /locus_tag="ACHA34_002461"
     CDS             9513..10064
                     /gene="raiA"
                     /locus_tag="ACHA34_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFM8793053.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3359 a         1669 c         2151 g         2906 t
ORIGIN      
        1 cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa aaatgtaagg
       61 ggattcctat atttttaagt atacttgttt ttccttttat acattttatg gtattatcat
      121 tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt atacggaatt
      181 gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct acttagcctt ctggtcttta
      241 cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat tagaatggca
      301 atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt agaagttatc
      361 gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac tattttaaaa
      421 gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga attttttgga
      481 gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg aacagtaaga
      541 attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt agcagaagaa
      601 ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat gaatcaagtg
      661 attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt aacaatcgat
      721 gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat gcgtgcttca
      781 atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc tatgcctggt
      841 ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca agctttagga
      901 gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt gatcggtaac
      961 acgatttatt tagatttccc tagtgtaggt gctactcaaa atatcatgat ggctgccgta
     1021 aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat cgttgattta
     1081 gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga aacaatgcgt
     1141 attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga tcgtatcgaa
     1201 gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat tgcagatgct
     1261 atctctgaac ataaccgccc attgatttct aaattgatcg aaatgggtgc agaaatcatt
     1321 gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac agatgtaaaa
     1381 acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc tatccaatta
     1441 gttgcagaag gtacaagtgt agtgactgaa acagtgtttg aaaatcgttt ccaacattta
     1501 gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc aatcatggat
     1561 ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc agcagcagca
     1621 ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt gaattatcta
     1681 gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga cgttgaacgt
     1741 gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta aggaagtggc
     1801 tgaatgagta accaacgata tatcttatta actttgataa agattttagt ggtgatctta
     1861 ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat cggtggcggc
     1921 aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt ttttcattga
     1981 taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca gaacatgaca
     2041 aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt aaaagaaaag
     2101 aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat cagcgcttta
     2161 tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat tatatgataa
     2221 aagcaattca aacccatggt gcactaaaag ggacggtaat ggggactgca cggatcttac
     2281 gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc caattaacgc
     2341 gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat acgagtagca
     2401 ctgagcgata aagaaaaaga ggaaaattat tgtcaaagta acaactccgc aactacttat
     2461 atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg tccttgttat
     2521 tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg agtggctgga
     2581 tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg gaaaaagaat
     2641 acgaaatcgt caatgtagca gtagccccaa cgtatcaagg aaaggggatt ggcggaaaat
     2701 tacttgagac agcttttcag aagctttcac aactcgcttc cagtcaaact cagatcatca
     2761 tacgaaccgg cagtactagc agtgcggcgt tacatctcta tcaaaaaatg ggctatgtgg
     2821 agattggtcg agtcaaagac tattttatcc atcattactc tgagcccatt tttgaaaatg
     2881 gagatcaatt aagggatcaa gtaattttag caattcactt acctaaaaat agctttaact
     2941 aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt acaatagctt
     3001 tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt ggtggatcgg
     3061 ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa tttgcaacgc
     3121 ctgaatcttg ggaagaaatg aaagcgcaat tatttcaagt ttataaaaaa atactcgttt
     3181 cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat gagaaacagg
     3241 tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc cgagatttgg
     3301 aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc ggaatggctg
     3361 aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg gcaggaactg
     3421 ggattggcgg ggcgttgttc acgaaaggac aaatcaataa aggggcacat ctttatggtg
     3481 gagaatttgg gttgatgttc ttaaatggcg ataagacatt cagtaaactt ggtacagcgg
     3541 tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaattct tataccggaa
     3601 aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa gttgagagtt
     3661 tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat ccagaagtga
     3721 tcgtattagg cggcggtgtt tctgcaaaag aagggctcat tgatgagatc aaaagccgaa
     3781 tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt ttattatgtg
     3841 aatacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct gtacgtcaag
     3901 gggagaatta agagctttag agaaggtgca gcttgcataa aagaagtgaa tgaaatgaat
     3961 aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata tctgagaggt
     4021 gaaaatgatg tcaaaaaaaa ataaagatgt tgaagtgcgt atagaagaag tgaaaaaaga
     4081 tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa tgattggtga
     4141 gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag attttggaaa
     4201 agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc tgcacgaata
     4261 aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat taggtattat
     4321 aactaagtca ggttgataca aacctttcgg aggagtagcg aagtggctaa acgcgacgga
     4381 ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat ttcattgggg
     4441 tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc gaatccagct
     4501 accccagtat accacaatcg taagctctcg ttagttcgag agctcttttt tttgctttgc
     4561 taccttgttc cttgaagatt ctgctgattt aagatagagt taagttaact acaagaattg
     4621 gagggatcca catgaaatgg aaagaaaaag tagacgaagc agctgaaaag ttatatgatt
     4681 tggtcaaatc agaaaaatac aatattgaag tgaatattcc gaaaaaaggc ggaaaagcgg
     4741 tccaggtaaa atcaaaacgc ccaacgaatc atacaaaaaa atggatggct aaaaaccgct
     4801 aataataaca aaagatccat aagtcggcat tttcatcgat tttagggtct tttgttattt
     4861 tctaggttga acatatctaa aaaattgttt ctgacaatag gagtaggaag aacgatgtgt
     4921 gtctaaatcc tctgttgatg gatagaaacc tttatctttt tcagttgtga atcaggtata
     4981 taccagttat aatagaagaa acgaaaggac gggtgtacgt tatgtcgaat caattacctg
     5041 tatatataca gattcatgac caattaaaaa atgaaatcga acaaggtata tggaaagtcg
     5101 gagaccgtct tccttcagaa agagaattag cagtgaaatt caatgtcagc cgtatgacgt
     5161 tgagacaagc aattcaaaac ttggcagatg aaggaatctt agaaagaaag atcggttcgg
     5221 gtacatatgt tgcgcgagaa aaagtacaag aaaaaatgtc tggtgcaaca agttttacag
     5281 aaattatgga atcgcaaaat cgagtaccgt ctagtcggac aatctcttat ttcttgactt
     5341 cgccaagctc aagtgaaatg gaaaaactaa acttgagtaa agatgacacg atcttgcgaa
     5401 tggaacgtat tcgtttcgca gatgatgtcc ctatttgttt tgaagttgcc agtgtgccac
     5461 aaaagctgat cgatggctac agcaaagcag agattactga atcgttgtat cgcacgctag
     5521 aagaaaaagg cgggaaaaag atcggtccag cgaatcaaac gattactgct gtgttggctt
     5581 ctgagaaaat tgctgactat ttagaggtaa aacgaggaga tgcagttctt cgtttgcgcc
     5641 agatttctta ttttgaagac ggtacgccgt ttgagtatgt gcgtacgcaa tacgtaggaa
     5701 atcgttttga gttttatttg gaaaaataaa aaacaggtga tccctgattc cttacttaga
     5761 aataaaagag aatcagaatc acctgttttt atatatgatc agttaccagt tgctcgtgat
     5821 aagaaacagg accctcttca aattggactt gtccgttcag cagctcaatg atggattctt
     5881 taaagtggtc agcacttttt tcatctacag cacagatgac gctaacttga tcagtatatt
     5941 ggatttcttt tataaaaaaa tgttggagtt caagataatt ctgtaatttt cctagttgcg
     6001 gatacgtgat catcacagag atctcctgtt gcaaggtacc ttctacgatt ccaatgtcag
     6061 acaatgcttg agagacagca tgagtgtatg ctcgtattaa tccgcctgtg ccgagtttcg
     6121 ttcctccaaa atatcgagtg actactgcag ctacattgat cagttgattt tttttgagga
     6181 cttccaacat cggtacgcca gccgttccac ttggttctcc atcatcgctg cttcgctgga
     6241 tttcattttt ttctccaatg atgaaggcgc tgcagttatg gttcgctttc caatgttctt
     6301 tttttatttc ttgaatgaaa ctttttgctt ggtcctctga ttctattcgt ctaagagaac
     6361 agataaagcg tgattttttg atctcaatct cactttgtcc attttctttt attgtacgat
     6421 agcgctcgat catcttgtca cctctttttt tcattttctt tattcagtat aaataaagtt
     6481 ctaggttgtt tcaacactgt aacggttgcg caatcgctgt agtatgcgat aataaagaca
     6541 aaggtaggtg gatctgggga tgcaggaacg aatgaaaaag tacgatgcaa ttacgcatta
     6601 tttgaagaac aatggcggct ctcaagtaac gttgaccttt acccaatttg acgaactgct
     6661 gtttcccagt aacggacttc ccaaaacagc aagggaaagt acagattggt gggcaaatga
     6721 ttataaacac cctgaaaaag gtgcctatgg ctggataaat gcaggctatg aagtagttgt
     6781 catcaatttg gacaaagaat atgtcgtgtt caataaatta gtgaaatcta gttggctatt
     6841 tgattaaata aaccgcgttt agaaaacgtt gacaaatttc tttcttttta tataatagag
     6901 taacagacgc gaagaaaacg tttgcgtagt ttgttttaaa aaggagagaa aagtatggct
     6961 aaaaaaacaa tcatgcttgt atgttcggct ggtatgagca caagtttact agtaacaaaa
     7021 atgcaaaaag ctgctgaagc aaaaggaatc gactcggata ttttcgctgt gtccgcttct
     7081 gacgcagata acaatcttgc aaataaagat gtagatgtac tgttattagg acctcaagtt
     7141 cgctttatga aagctgattt tgaaaaacgt ctggaaccaa aaggcattcc tttagacgtc
     7201 atcaatatgg ctgattatgg tatgatgaat ggcgaaaaag tgttagatca agcaatcacg
     7261 ctcataggtg aataaataga acagtataac tatcgaacaa gcgatccaaa aaatagatcg
     7321 cttgtttttt cgtatatttt tttagggcga ataatatgaa tcatttttgg ggtagaaggc
     7381 ttctgacgag agatattaaa gcaatagact gtgaggaggt aaatttacca aattatgaga
     7441 agatcgcagc agtggaacgt ttgggcaact ggattctttg tcatcgctgt ggaatgagga
     7501 cgtccgtcat tgatggacag cttgcagatt acggttattt ttgtacacat tgtctatatt
     7561 tagggcgatg cgacactcaa caagatttat atttgtttga tcagcctgaa gcaaaacctc
     7621 gagaagtcgt ctttttttgg acaggacagc ttactgaaaa acaaacggaa atcgcaaaga
     7681 gaatactttg ccattcagaa aaaagacatc atcttatttg ggctgtaaca ggtgcaggga
     7741 aaacagagat gctttatcct attttagtgg aaactttaaa agctggagga agagtagcga
     7801 tttgtacacc gcgagtcgat gtttgtaatg aattgttttt acgctatcgt tatgtttttc
     7861 cagaagaaaa aatcacgttg ctccatggga atacagcaac ggcttatacc tttacttctt
     7921 ttgttatctg tacgattcat cagctatacc gtttttatcg gtcctttgat ttaatagtga
     7981 tcgatgagat cgatgcgttt ccttataacg gggatgcagg attagctcat gctgtgcaaa
     8041 cagcgattaa accagacgga cgattcatct acttaagtgc cacacctgat aaaaagttac
     8101 taaaggaaat caagcagacg tttgacatcc acaaacttgc tttgcgcttt catcggcgtt
     8161 tacttcctga acccgtcttg gttttcatga ataattggcg acaaaaatgc aagaacaaga
     8221 agaagataaa atctttggtg cggatcattc atcaattaat taaagaaaac catatccttt
     8281 tattttgtcc aagtatcacg atgatgcatc gtttgaaagc cacacttcaa gaggctcttc
     8341 cgagatatga gatcacagat gtttcttcaa gagacaatca gcgggcggaa aaagtccaga
     8401 aaatgcgaga agcaaaatat gacatactgt taaccacgat gatccttgaa cgtggagtca
     8461 catttgaaag aatatccgtt atcgttttag gagcggatca tccggttttt actaaatctt
     8521 ctttggtaca aatcgcagga agagccgatc gtaaagggcc ttatacgaac agtcaagtat
     8581 actttttcta tgaagaaaaa actgcagcta ttcgaaaagc ttgtcaggaa atcaaagaaa
     8641 tgaatgaaga agggaagaag atgctgtgag gtgcagttgc tgcagtaaag atatcgtcca
     8701 aaatttaaca ttcagcgaga ttattggtgt ttcgaaatac agattacctc agacttgttc
     8761 agtttgtcgc gcacaatttc atttattgga aacaagaggc tgttctggct gtcaaaaacc
     8821 aatccatttc aatctagaga gtacgaaaaa gaaagtaaag tctattcccg tttactgcag
     8881 tgactgtcaa gaatggaaga agaaatatcc gacctatgat tttcagcacc gtgccttttt
     8941 tgtgtacgat gaaaatatga aggcctggct caatcaatac aagtttatgg gagatatccg
     9001 actagcaggc acctttgcat ataaatggac gcagataaaa aaagaatacc cgacatacat
     9061 ctattgtcca atacctttat ctgaaaaaag gcgaacagaa agaggattca atcaagtaag
     9121 cgagatgttg aaagcagcaa gaattccttt tcagatgcta ctcaaaagag agaagcatct
     9181 gctggctcaa gcaaaaaaca cgcgaaagga aagattggag atgcctcaac cttttgaact
     9241 tgcagtcacc ttttctaaga tacaaaataa aaaaatattg attattgatg atgtctatac
     9301 aacaggacag actcttttcc acgcagcaga ttgtttgcag gtcgcacagc cgaaaatcat
     9361 ccgaaccatt tctttagcaa gatgaattag atgtggaaat ttgatagcga tttaatttgc
     9421 aaataaccta gagttcgata taatagaatt aagaagaggg aaagagtggt ccttctcctt
     9481 cttgattgtg gtagacgaaa ggggtaattt ttatgtttag atataatgta cgcggcgaaa
     9541 atatcgaagt aactgaagcg attcgcgact acgttgagaa aaaagtaggc aaactagaac
     9601 gctatttcag tgacgcaccc gatgcaacgg cgcatgtgaa cttaaaagtt tatacagaaa
     9661 aaacagcaaa agtggaagta acgatcccat taccttatct tgtattacgt gccgaagaaa
     9721 cttctccaga tttatacgca agtatcgatt tggttgttga caaattggaa cgccaaatcc
     9781 gcaaattcaa aacaaaaatt aatcgtaaat cccgtgaaac gggcatgaat actgcagatg
     9841 ctactgcttt attcaatgaa gaggacgaaa acgaaaatga ttctgaatta gatatcgtac
     9901 gtacgaaacg cctttcattg aaaccaatgg atagtgaaga agcagtctta caaatgaaca
     9961 tgctgggaca taacttcttt atttttgaag atgcagaaac aaatggtaca agtatcgttt
    10021 accgccgtaa agatggaaaa tacggactga ttgaaacaga ctgatcagcc ttacttaaat
    10081 aacaa
//
LOCUS       DAYYFU010000081         9690 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000081 DAYYFU010000000
VERSION     DAYYFU010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.086"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1283)
                     /locus_tag="ACHA34_002462"
     CDS             complement(<1..1283)
                     /locus_tag="ACHA34_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8793054.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTK"
     gene            1712..2245
                     /locus_tag="ACHA34_002463"
     CDS             1712..2245
                     /locus_tag="ACHA34_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793055.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(2371..3048)
                     /gene="nth"
                     /locus_tag="ACHA34_002464"
     CDS             complement(2371..3048)
                     /gene="nth"
                     /locus_tag="ACHA34_002464"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFM8793056.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3055..3753)
                     /locus_tag="ACHA34_002465"
     CDS             complement(3055..3753)
                     /locus_tag="ACHA34_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8793057.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            4021..5205
                     /locus_tag="ACHA34_002466"
     CDS             4021..5205
                     /locus_tag="ACHA34_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFM8793058.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(5211..5777)
                     /locus_tag="ACHA34_002467"
     CDS             complement(5211..5777)
                     /locus_tag="ACHA34_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8793059.1"
                     /translation="MTSFVNPFFSWFQIGFSYKIFLSLLNRKQKNNIMKEKEGVEMDK
                     RQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHIGKIPDPNVTKLANASYM
                     TRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKIRNRHEQLHQKWVQRDAT
                     VFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5880..6167)
                     /locus_tag="ACHA34_002468"
     CDS             complement(5880..6167)
                     /locus_tag="ACHA34_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8793060.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(6338..7690)
                     /locus_tag="ACHA34_002469"
     CDS             complement(6338..7690)
                     /locus_tag="ACHA34_002469"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFM8793061.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(7963..8409)
                     /locus_tag="ACHA34_002470"
     CDS             complement(7963..8409)
                     /locus_tag="ACHA34_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8793062.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(8645..9571)
                     /gene="cysK"
                     /locus_tag="ACHA34_002471"
     CDS             complement(8645..9571)
                     /gene="cysK"
                     /locus_tag="ACHA34_002471"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFM8793063.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2667 a         1934 c         1626 g         3463 t
ORIGIN      
        1 ttcttagttt tcttgaaata ttttcctcga tataaaaaga ttcgttctcg gtagttttga
       61 aagtttctaa agccataggc aattcgtttt agtactttga tatggttgtt caaacattct
      121 atagggccgt tggaataggg aactcgcaac gctcgtttga tttctgttcg atatttcttg
      181 aaggtcgtga acactggttg atagtgctct ggcaactcct tgtagtcttc ttttaggagc
      241 gcgacgaatt caggaacatc ttgtcttctg atagcggaaa gaaaaacctg gtagacttcg
      301 tagcctcgct ttaaggagtc atcataatat aatagccgat cgactatctc cgcttctgtc
      361 agatgatctc gaaatgacgg acgccagtag cgatgctgac cattcaattc ccacgcattc
      421 ttttgaagta gtctccagta tttctttaag tgcttcgacc gacgatcccc ttttttaaag
      481 gtcttcatta cttgaattcg gtggttggta aaggcccgac cgatgtgttg gctaatatga
      541 aagcgatcgg cgacgattcg ggcattcgga aaacactctt tcaccaatgt gcgatagggt
      601 gtatagaaat ctgaaaccac tagacgtact tgttcacgga cttttctagg gtagcgcaag
      661 aaatagtcgc gtaaaggatt gagttgccga ttttccacaa tatcaatcag ttgatgggtc
      721 ttgccgtcca tcatgacaaa gctcatggct cccgagacgt ttttcaccga tttgaattca
      781 tcgaaacaca ccacttctgg taacggctgg ttcaaagaaa cggttttagg ccgaaggctg
      841 cgaagtattc gaatgacagt ggtcggagaa atgtgcttca tacgtgcaat caaagacata
      901 gaaataggct cacttaatag ctcaagaatg gcttgtttga ctctgcgggc aatggagcag
      961 cgacgatcgc taactgaatc ttctgctaaa aaagtctttc cacagtgttt acatttgaag
     1021 cgctgtttgt taatccgcac aatggtcttg tagttactga catcattcaa taagatggat
     1081 acttttttcc agccccattt aatgatcgtt tgcttatttt gatagtggca atggaagcat
     1141 tcctctggtt gataggtcaa tgtgccatca aaaacaaaac atgtttcgtc attaatgcgc
     1201 tctttgcgaa aggcatcttc atgaaaggta agatttaggt ctagaatatc tagtaactct
     1261 ttggtaaact ctgaatagga catcttgaaa cactcctttg aatggttttc gtcgacttta
     1321 ttctaaagga tttcaaggtg tctgtttgcg tttattagaa aaaagaaaaa gtattggcaa
     1381 cagaaggcgt caccaacact agatattata gatcctaaaa aagaaaccac gtctacaagc
     1441 gtggtttctt ttttgtgtct atgtaaaaaa cagctacaaa taaaaatcat gtaccaagct
     1501 gtattacatg attttttata agactatcca gcatatacat gtgtgatcta acatttagct
     1561 taaaaattag aaaatccatt tttcttttat cattacatat actttatcca tttacttgtt
     1621 tataattaaa tcggctaaat acacactagt cttttattta gtgccacttc ttaataatcc
     1681 cattgttatt atcaaataaa aaatacaata tatggttttg aataattttt tgaaagaagg
     1741 cttcattttg agttatctga tagtatgctt aattatcata atttgtctgc accatctttt
     1801 tttatcgaaa acaatacctc gttattccaa gaataaaaaa gctgaaaaat tttgtttatt
     1861 attaaataaa tttactttaa ttgctcctat ccttgcattt attatttttt ctgtactttt
     1921 aagcactact cttaaaggaa aatttatgga aagaagctct catgcaatga ttctcacttt
     1981 tttatggttg ttgttcacca gaatctatat ttttctgatg agcctgaaac cgcccaaatc
     2041 cattagttta tgtttagtta taaatggtat ttttttatta tcacttatca tttttattac
     2101 acctttagat agatacgtca cttatttata taatcctttg gaatactgga cttattttat
     2161 aggtatattg gaaggcatta ttttctacat cgggtatttt cccaataaaa ataacaactt
     2221 ttatttttat cgcaataaat tgtaataagt taccatccta gctactgagt atgtacattt
     2281 actttttcat gcaaaaaaga taaacgaaac agcttttctc cataataaca agataattca
     2341 tacatttatt tgcaattaaa gaaaccacgt ctacaggcgt ggtttctttt ttagcgcctt
     2401 ttcttttaga cgcatccgat ttttcccatc ttgacatatt gatagcaaag gacatacctc
     2461 gcacttggga tttcttgccg tgcaatgata tctaccaaaa aaaatcagtg tatgatgtgt
     2521 ctttacccat aattcttgtg gtactttccg catcagtgtt tcttctactt ccatgaccgt
     2581 agcatctagt ttgcagatcc tcaggcgttt tgagacacgt tccacgtggg tatctactgc
     2641 gattgctggt atcccgaatg catcccccaa tacgacattt gctgtctttc tgcctacacc
     2701 aggaagagac atcaattctt ctcttgacgt tggcacttgc ccatcgaaac gttcaatcag
     2761 ctgctgtgca cacgctttga tgttttttgc tttatttcga tacaaaccaa tcgtttttat
     2821 tttaagaatg atttcatcga ttgaagcttc tgctaacgca tcgggtgtcg gaaaagaggc
     2881 aaacaaatct ggcgttgctt tattcactga tacatctgtc gcctgggcac ttaagatcac
     2941 tgcaatcaat aactcaaatg gattattgtg cttgagttct ccatgtgctt cgggaaacat
     3001 tccatacatc gtttctagag cttccattgt tttttgtttg cttaacatat tccgctactc
     3061 ctccaaccaa tttttcagtg agactttcgg caattcttct tgttgagctg actgttctac
     3121 ttctctttga agaagctgct gttttcttcg tttttggtct tcttccactt gttgttttgt
     3181 acgtagattt tttctttccc acgatagcaa aatgcggtca acgtaattaa aactataagc
     3241 ctgatttaat acggcttccc gcagtgctag cttcaaaatt tccggctgat aatggtcttc
     3301 ttctagccat tgtccgatcc gctggaactc gatcgaagaa agcgggcgtc caaattcttg
     3361 ttcaaataat tgatacactg actggatttc tttttcttgt gttttttctt cgtatttttt
     3421 ttcttgtgtt tttaaatact cacctaatag atcatagatg gggtaaagat tatatcgatc
     3481 tgctttttta ccgttgacaa tcgtcgtttc tatcttgata aatccatttg ttacgagacg
     3541 atccagtatt tgaaaaatct gatcttgttt gattcccata tccatagcaa tcatctgcaa
     3601 atcagggaaa gtatctcctt caagctgtgc catatgcaac tgtagaacaa ataaaaattc
     3661 ttcagattgt agaccgatcc tatgataatt tttaagtaaa agattagata tgactgtttg
     3721 tccagcgctt aagtaatttt ctaaattcaa catgatttct cctccgctca atcacagtat
     3781 acgtaaaaaa aacatgggaa gcaagaacag gtttcttttc tatttacttc actgatcggt
     3841 cagatttaca atgtcgtcta cttgattttt gtttgttcat gtttttgatg cgtatcttca
     3901 cttttcggaa taaataaatt aagtagattt cgctgttcct tagaatgttg acaaggaaac
     3961 aaaattacta tacactattt ttgtttccaa gttaacatta ttgcataaag gagtttttac
     4021 atgtacagtt tcacaaataa acattctaaa gtttttggat acgtctctgt cttattcact
     4081 ggattaggat ttactatcgt cagtcctgta cttccgtttt taacgctgcc ttattctcac
     4141 tccatacatc agcaagcttt ctatatcact ttgctcatgt ctgtttatgc tctagctgcc
     4201 ttcttatctg cccccattct cggtagctta agtgatcatt ttgggcgtca tccgatcctt
     4261 attatcagtt tattaggatc aagtatcgga tatttgcttt ttggtttagg aaattcgata
     4321 tggatgttgt tccttggtcg tatcattgaa ggattgacgg caggagaaat ctccacctta
     4381 tatgcttact ttgccgacat cacggaacct aatgaaagaa ccaaagtttt tggatggatg
     4441 ggtgctttag tcggtatagg aacaacgctg ggtcctatta tagggggtct attagctgag
     4501 ttggggaatt ctgttcctat atttattgga gcactcttta ctttcttaaa tgccgtttat
     4561 ggctacacat ttatgctaga aagccttcca ataaaaaaac gttcagtaga tttaagtttc
     4621 tcccatgtaa gaccttttca ccaattacaa caattattca agatttcatc cgttattcct
     4681 ttattgacag caggttttgc tgtttggttg gcagctggct ctcttcaatc tatcttttct
     4741 caattttcga ttgacgcctt tcagtggaag gctgggttgg ttggtttaag cttctctttg
     4801 attggaatct tggatactgt ctcgcaattg ttcattatgc ctcgattgct caaaaaattt
     4861 tcagaacagc agatcacaag aatagggatg ttcagtgaaa tcctcgccta tctattcatc
     4921 acattatctg gaattcttct actgccgatt ttgtttttat ttgggattat ctgttatggt
     4981 ttcggtgatt ctatttttac tcctgtattc aatggccaac tatccaattc tgtctcagat
     5041 aaccagcaag gtcttgtaat gggcggtacc caaagtatac aatctctatc gcgcgtcatc
     5101 ggcccgctaa tcgcaggaca gctttatgct gcagcaccat ttcttccgac ggtattcgga
     5161 tttttgctta tgatttttgc ctattatcac tacaataaaa aatagtagct tcatttgcta
     5221 cttcgtataa attctctgta tcgaccgata aaacgaaata ctgtgtcaaa ttcttctttt
     5281 gacatatttt caaatacagt agcatctcgc tgtacccatt tttgatggag ttgctcgtgt
     5341 ctattcctta ttttttgtcc gattggcgtc aattgaaaat agatttcttt tttattctct
     5401 tcgctttgat aagtgtttat gatccctcta gctatcagtt tttttgttag cttactgatg
     5461 gctcctctag tcatgtaact agcattcgct aattttgtca cgttaggatc agggattttc
     5521 ccgatatgtt caatcacttc tagttcattt agagtaaatc ctgcaaattc tttttccatt
     5581 tctggtttat tacgatgaat aatctcatta gctaaatcca gatattcttc tattagcagt
     5641 tgtcttttat ccatttctac accttctttt tctttcatta tattgttttt ttgtttccta
     5701 ttcaacaaag ataaaaatat tttgtaggaa aacccaatct gaaaccaaga aaaaaagggg
     5761 ttgacaaagc ttgtcataac ccctttttct gattaaaaga tagtaaagag acagtcatcg
     5821 gtaattgtga tgattttttg tcatcactta aggatgagcg gccttttcaa aaactgtctt
     5881 taaagatcat catcagtttc tagcacatat ggctgacctt catatgtgta ctctgtattc
     5941 aatgtgccgt cattttcatc tgagtagatg cgtctactga aaggaaaacg atgagaaaca
     6001 ttatctttgg cgtcatttgc tggcatcgtg taatattcct cttgcgtttc attcaactga
     6061 gagagtactt gatctgccaa ctcatcaata ttgatcgtta atttcttcga agcactttta
     6121 taatcttcta aaaatgcatc ttgttggaca aaatattctt tattcatctt ctttctcacc
     6181 tttcttttca aaatcaaata aagctgcttg ttcttttatg taacagcttt atctttgctg
     6241 cttattgtga caaaaaattc gaagaaagac aactaattgt acttttcaca aaaaaagaca
     6301 gagggtcgtg acaaaattgt gtcacaaccc tttttcttta ttgtgcagtt tcgatttcgc
     6361 ctagcgctgc ttgtgcaagt aggttcaagt aattccatgg gcggtcaaaa tgtggttgga
     6421 agaagaaatc tgaaagtgcg agatcttcta cagtcatttt attttgaaca gctaaagaca
     6481 tggtatttgc tgattgtgta atgtcgtatt tggacatgaa ctgagcacct acgatacgat
     6541 tcgttccttt ttcatagact agctccatca ttactttttc agtagttggc atgaactctg
     6601 gacgataatt atcttcaaat acagtcgctt ggacttctag atcgttcaaa gttgcgcttt
     6661 ctttggtcac acctgtcgaa ccgatcgtcc agccaaatag gtaaagtcct gaagttcctt
     6721 gagtaccacg atacgccatt ttatgatctg tcaagttatt tcccacaagc aaaccttgac
     6781 gaacggcatt tgttgccaaa ggaatatagt tttgcgtgcc acttggattg tagttgacta
     6841 ctgcagagtc tccagctgca aaaatgtctg gattacttgt ctgcatgtat tcattcactt
     6901 tgattgcccc gtttggcagt gtatctactt ttccttcgat cagttttgta tttggacgga
     6961 agcccacaca taaaatgacc atatctgtat caaatgtatc tgttggtgta gctacttgtt
     7021 tcacttttcc ttcttcatct gtgataaatt cagtaacatt ttcgcctagc gcaagagtaa
     7081 caccgcggtc tgtcaattct ttttctaaga tatctgtgaa tggtttatct agatatttat
     7141 tcaagatgcg atctaatcca tcaatcaatg tcacttgttt tccagattcg acaaaagctt
     7201 ctactaattc aataccgata tatccaccac caacaattac tacacgtttt gcttctttcg
     7261 cttgtgcaat aatttcattt gcttgattgt agtttttgca taggagtaca tttttcgcat
     7321 cgattccact aatcggtggg ataataggcc atgaaccagt tgtcatcact aatttatcat
     7381 aagaaaccgt ttctgtttct cctgttttca gatcttttgc cgttactgtt ttattttctg
     7441 tatcgacgtc tgttacatcg tgttccattt taactgttgc tcctaatgaa gataattctt
     7501 caggatttga gtagaacaag cctgctgggt cctttactac tccgccgaca taaagtgcga
     7561 taccgcaaga taagaaagaa atattgtcat ttctttcata tactgtcact tctgcttctg
     7621 gatgattttt taaaatactt tttacagctg cagtacctgc atgagtacag ccaattacta
     7681 ctactttcat ttatggtccc tccgattaac attttgtcct tcgacaaaac tactataaca
     7741 gaaataaaac ggatacaaca gtttaatgct tgtgaattca caaaggtttg tttgtagttt
     7801 cattcctttt caagcataag aacccaatat ctacaagatt cttattttaa taatattcac
     7861 ttttatatct aagattgtga ataaaagaca aaaaaataaa aaagctagcg ttttttttac
     7921 acagctagct ttttttaggt ataacacttg tagaaactgt ttttacattt gcgccttttt
     7981 tgctgactct ttacaatccg gacagagtcc atacaattcc aaccgatgtt cattaatctc
     8041 aaaatcagtc agtctgcttg cagccatttc tatatcttca agccctgggt aatgaaaatc
     8101 aacaatctta ccgcagtttt cacaaattac atgataatga cgctttgaac tgaaatcaaa
     8161 tcggctagaa gcatcgccgt acttcatttc ttgaacaaat ccaatcgaag taaataagcg
     8221 aaggttattg taaacagtcg caacactcat atttggaaac cgatgttcaa gtgctttata
     8281 aatctcatca gccgttggat gggaatgatg ttcaatcaaa tattctaaaa ccgcgtaccg
     8341 ttgaggtgtg attcgaatgt tctctttttt taacttttcg ataacctctt cgactaactt
     8401 attttccatg taaacacccc acgttgttat tctcgtctta tcatactatt tttacatttt
     8461 aaaatccact taacatgaaa agaaaattca attttattgt attttaatga aaaaagacgt
     8521 aaaacagtcg cgttcactat tctacgtctc tattcaaata atttttctca tcataaattt
     8581 ttgtgtgttc ttttcatctt taaaagtttt ttacataaac ctatctggag aaaaaccaag
     8641 tgccttatat ctcttggtaa agggctgtag ataaataacg ttcaccattg tcgggaacca
     8701 cagctaatac cttcttgcct tttcctaatt cttttgcgac ttttagcgcg gcagcaattg
     8761 cagcgccaga tgagatgcct actaaaatcc cttcttttcg cccaacttct ctagctgttt
     8821 ccatcgcttc gtctccacta atagaaagga ctttatcata cacagaagta tccagtgttt
     8881 taggaacaaa acctgtaccg attccttgga ttttgtgagg tcccgcttct ttcccttcta
     8941 aaatagcaga ttctgctggt tctaccccga ttaattcgat tttcggatac acacgtttta
     9001 attctcggcc agcgccagtg atcgttcctc ctgtaccgat acctgcaaca aacgcgtcca
     9061 agccatttac tccaaatgct tgatgtatct caggaccagt cgtcttctca tgcacaagag
     9121 gatttgcttc attttcaaat tgtaatggta aaaagtagcc gttttctttt gccaaacgtt
     9181 ctgcttctct gattgatcca gaaataccat ccgcaccagg agtcaaaatc aactctgcgc
     9241 cgtacccttt catcaataaa cgtctttcga tactcatcgt ctcaggcatc acgatgatta
     9301 ctttatatcc ttttgcaact cctaccatcg acaatccgat tcctgtattt ccagaagtag
     9361 gttcaatgat agtatcacct ggtttcagta gaccatcgtg ttctgctttt tctatcatac
     9421 ttaaagcaat acgatctttt acacttcctc ctggattaaa aaactcaagc tttacaaaaa
     9481 catctgctga atcttctggt acgattttat ttaatttgac gattggcgtt ttgccaatca
     9541 attcggtgac tgagttatag atagctgtca tcttcaattc ctccttgtct ttgctcttct
     9601 tacttatcat aatacaatct gttcatttat ggcgagtgat tcgtctttga ctagcaaaaa
     9661 aaagacaaag atataccata tatggtaatt
//
LOCUS       DAYYFU010000082         9378 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000082 DAYYFU010000000
VERSION     DAYYFU010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.087"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..414)
                     /locus_tag="ACHA34_002472"
     CDS             complement(<1..414)
                     /locus_tag="ACHA34_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8793064.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLP"
     gene            complement(404..2578)
                     /locus_tag="ACHA34_002473"
     CDS             complement(404..2578)
                     /locus_tag="ACHA34_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HFM8793065.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2594..4936)
                     /locus_tag="ACHA34_002474"
     CDS             complement(2594..4936)
                     /locus_tag="ACHA34_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8793066.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4958..7501)
                     /locus_tag="ACHA34_002475"
     CDS             complement(4958..7501)
                     /locus_tag="ACHA34_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8793067.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7511..7909)
                     /locus_tag="ACHA34_002476"
     CDS             complement(7511..7909)
                     /locus_tag="ACHA34_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFM8793068.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7990..8274)
                     /locus_tag="ACHA34_002477"
     CDS             complement(7990..8274)
                     /locus_tag="ACHA34_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793069.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8298..9296)
                     /locus_tag="ACHA34_002478"
     CDS             complement(8298..9296)
                     /locus_tag="ACHA34_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8793070.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2670 a         2005 c         1468 g         3235 t
ORIGIN      
        1 aggaagaaat tgattcaatg tcttttcaaa aaagaatgtt tttctctcaa tttttttgac
       61 gtttctatca tgaatcacga gaaaacttgg gacactttta acatggtaac gttgtaaaaa
      121 agtttgaagt tcttcttgtc ttggttcttt tcttgtttca attcgatata cattcctaga
      181 gaattttttg tcgtatcttt ccaagaaaga ttcaacttct tggcattttt tacaatcatt
      241 tcttgctaga tacacaaaaa atgattgatt cgttttttga agttctagta cttcttctaa
      301 attactattg attagttttc catttgattc ttcgttagtt tttatcagtt taaaacaacc
      361 aaataagagg aaactaacaa taagtatcct aacaataact ttcttatttc gcatattgtt
      421 tctccaaatt tggtttccga atatagacga ctcgcggtgg ttcctggacg tcttcagctg
      481 ttcgatacac cacaaattcg cctgttctca atgctcgaat gcgattcgga ttgaatttaa
      541 agcggtctac gtctcgtttc gtgcctcttt ctcctgtcca atcaaccttt cccatatcag
      601 aataacctgc ttcttgtgtc gtcatttccg ttaattcaat gtcttcttct gtccccatta
      661 aatctgccca ataatcaatt tctgtcgggt catttgtctt cccaataaag aacgtattca
      721 cattcccaat caactgtttt cgaaggacag gatcaattct atcaatgtcc gcaagcgttt
      781 gaggggcata cgccgcatgc accccagcgc ctcgtccctt attgactaag tctataaggt
      841 tatcatttag ataagaagct ggttcgtcaa atattactag gactttcgag cgattggctg
      901 tttctcctgc ttgtttatac acttctgacg caaagaaatt aatatctccc acaagcattt
      961 gagccaatgt ccgaataaac tcactataaa tcaatccgtt taacgagata taaacaatct
     1021 ttccttcttt aacaacttct tgaatatcta attctctttt tcctccagaa gtatcaaaga
     1081 gttcaccaag ctcactatcc aataacatat tgatattggt ctgtaaattc tcagaagtcg
     1141 catacaaata aaacgggttc ttcttatgtt cgtaacgaac aaacaaccgt ttaaacaacg
     1201 tttgctcgat ttctgtcatg agagaaatgt tttcttttaa taatagataa tacgtttcta
     1261 agtcaacggt tttacgatca tgttcaacga ttcgctcttc acttaactgt tccacatcgg
     1321 caaaatcacg ttctgttttg tccatcgctt cattcttttt cgtttttttc ttcgtggttt
     1381 tcttcttttc tacgaccact ttttctttaa ataaatctgg atttttcaac tgaatacgat
     1441 ccgcaaacaa atgtaagacg tttcgtggca ataaataatg ttgtaaccaa actagatttc
     1501 tttcaatttt atggctcttt tggaactcgt ctaaaagctg aatcgttacc tgtaataacg
     1561 ctttggctgc tgtcgaataa tacacgcttt ctgtttctgc tagattggtt aatttatctc
     1621 gaatggcaat atcattgcca ttttttacag gattataacg caaatcgcca ccgtccacaa
     1681 agacttcaat cgggcgattg tatgcctctg ccacttcttt taacgcttgt aaagtctctc
     1741 ctgctccttt cccatcgact aagataacgg ggatacgaaa cttacacgca tgttctgcta
     1801 gtaattggat caccgtcgtt tttcctgaac ctgtagtcgc tggaataatc acatgttggt
     1861 tcaattcact aaagttcatc acataaggga gcccatactc atctgttcct aatagaatca
     1921 ctttggctaa tgaagccagt tgtttattcc gcaaagcaac gtcttttatg acaattgcta
     1981 cctggtacgc ttgctgatac tctgctgtat atttttggga ctcctgaaat ttattcttcc
     2041 gacgttcctt gtattcttta ctatttagca aagctgtttg ccgttctttc tttgaaacaa
     2101 cttttctttt ttcaataaaa gacgcatacg caatcaaaca acttcctgca cctgtcccac
     2161 ctaaaaggag ggcaaaaatc gtttgtccat taaactgcaa cgcatgagaa aacaagttta
     2221 atggaaataa taagtaacac gacaaatacc ctctaggaag aacaaaaata aaacaaaata
     2281 gatttaatag cgtaaaacct aacgcaaaaa taaaaaaaag agagcttctt cgttttaaga
     2341 agctctcata ctcaaagtca taaatatgtg cacgtgtctc ttttttatct atcataaaga
     2401 caatcgctaa aggaatactg cctacaataa aaaatggata tagacaaaca cctaataaag
     2461 tgacgataaa ataaagttgt tgaaaccgac tttttggcgt ttctttttct tgttttcttt
     2521 tgcgatcgac agaaaacatt tgctcaaaat gtgtcggtcg ttttggtgca ttcatcatac
     2581 tatcgatttc ctcctatcgt tttcgttgtt tcatttctct tcgataaatc ggttgttttt
     2641 ctcgttttcg tttcattttt gcttgagttg ctcgaaactc actccgtgtc ttcggtgctt
     2701 gttttggtga ttttgtttgt ttcatggttg agtatgcttt agatacggtt gaaggcgctt
     2761 tggtttgtgc tgttactcga ttgactgctg acatatgttc ccgaaccccc tctggacgtc
     2821 gaacagattt cggtgtggta gaacctgttg ctcgtggtac attccctttt cttagtggtt
     2881 tttgtaccgc atgtttttgt cctgcaactg tccgggtttt catcgaactt gtttttgtta
     2941 cgggagtaag tgcctccttc cgacgacgac ttgtttgtcc ctgacgacgt tttcgatcgt
     3001 tcaattctgt tctaggcgta tgcttctggc cataagactt gtgtttttgc tttggattcg
     3061 ctaatgacac caccattgca gaaccctgat tgcgatacgg actacgagac gcaccttttc
     3121 tacttctttc tcttagacta ggattgcctt gcaattctcg ttgtaataaa tcggctgttt
     3181 ttcttgcctg tttctccatt gctgttcgtt gcatacgttc ttgactttgc tcttctgctt
     3241 gtttcgcttg atcatgcaag tctcttgctt tttgtgccat acgttccata cgatttggtg
     3301 ctttatgacg aggttgttgt gcggattcac gccgaaaagc acttcgtgtg gcttgtttcg
     3361 ccgtttgtgg catacgaacg tctgagccac ccccacgttt tcgttgacgt tcttgtctct
     3421 tccgtgcgtt ttctttttct ttttgtttct gtgcttttct cgcttctttg cgttctttca
     3481 accgttgttt ggcttccctt ctcaattttg cttctgcccc aaatggattt cccgcaaaag
     3541 ctaacgctcc tcgaacaccc attcttccag taaatagact gcctaaaaag cctcggaaca
     3601 aataaattcc caacggcgca aacaaataaa caatgatcaa gagaattttt tcccatggat
     3661 cgtcttgtgt actacttaat tggaacccgt aactaagaaa agaagaaaaa aggatacatg
     3721 caaaacctgt cattcctttt aaaaacgtca aaataccaaa aactttggca tagttaccaa
     3781 acacattcat tgtgacaaga aacaatccta aaaaaagtaa gaacggcaac atcggtacta
     3841 acaatagttg catagttcca ataatcaatc gaaacatact taagacaaaa taaacaacgg
     3901 tttgaatcag attcacgaca aaataaaaca aggcaaatgc tgtatttttc caactattgt
     3961 aatattgtat gttcttgttt ttcagtgtat ctgcttcata ctctgtgact ttgtcatgaa
     4021 ttttctcccc tatttttgta cctaaatcat tgtccaataa aagattgatt cgatcatatt
     4081 cttttccagc atatttgact ttcttctcac gaattgtgtc tacattggtt gtgccataat
     4141 ttaacaacag atatggttca taaacattac tataaaagac ccgagccgcc atcaatttgc
     4201 ctgccgtttc cactttttta tccgctgtta cctcttctgt ttcttttgtt tcataatttg
     4261 tttcttcttc aggtgttaac actggattga ccttcacgaa cgccgtttcc actgtagaat
     4321 ctaaagaaaa aagaacatca aaaagattct tatttgtccc agaatctttt aatacgctta
     4381 acccagtaaa aataaggatc gtcataagaa agacacggag tgcttgtttc attcgagcct
     4441 ctccagcata tttgattgcc atcataattg ccgaaaaact gattccaata gttcctgcaa
     4501 tacttaacat attgtccgca attttttgtg ttaagttttg aatgggttct cgaatggcgg
     4561 aaataatatc taattcaaag agaaattcaa ccattgaagc attgaattct cctaacattt
     4621 ttacgccact ccaaacaaaa tttttcatca ctcctgcaac attgtacgat agcttttttt
     4681 taaggttcat tgactcatag tcattggtca tcaattcaaa cgaattcttt ttgtaatttt
     4741 tatacaattg atccacatca gtcgttactt cttttacttc tactttatcg ttggcattct
     4801 ctccttcaga acgatcggtt tcagtgacac ccgcaccatc attcatttga tcggcagaaa
     4861 cagtagcccc ttgtagacag aaaaaaagac cccctaataa aaggagcgtc caccatttca
     4921 gagatttttt tcgcaaaaaa accacctctt ttttgtttta tcgttgtttt tcattttcta
     4981 acattgcttg aattttttca tcttgatttt cagtagaaga aacagccaat aaccaactat
     5041 caaacaacac atcaatggca atcggttgat ttcttcccaa atgatcctgg aataaacagg
     5101 ttcctgtttg taattgttcc atcatttcta aattggctga attcacttcc atatcaaaga
     5161 aggacaatag tttctctcgt gcttcttttt gtttcggacg aaacgcaaat ttgtaagaaa
     5221 ttaactcttt tttatcttct tgatcgtaat ctttaaacgc ttgcacaaca aggtaaatat
     5281 ccgtattatt cgcacgtcct tttcgcaagc tatcatcaat caaatagctt ccttgtgcgg
     5341 tatcttcata cccttttgcc tcgtcataaa taatcgcagc gtcttcttct ttgtttgtag
     5401 aaaagatata cgtaaacttc ataatgactt ccataattgc cattcctgca agttgttcgc
     5461 tatttaatcg acccgaatca atctcttttt gtgtcggaat gcgtattcct tgtgtcccta
     5521 acacgttgat ttgtgaatca aaacgtatcg gcgtactgtg ctcattccca atgagaatct
     5581 ttcctaaacc aacgctatgc cctaaaatca atttagctag ttctttgtca ctttttcgaa
     5641 tttcttcgat aacttgactt aacgaatgct tacctggcat ggtgtaaact acatcgatgg
     5701 cgtcttggat caccgttttc ttgtggctcg ctgtttctcg attggtatct acttctccta
     5761 acattcctaa cacattttta gcggtttgaa ttgcctcttc ttttggtaaa aacaatagag
     5821 gatcaagcat ccctcgacag ctttcatctg aagataacga aataaaatta attcgttcat
     5881 acatagccac aaactctggt atgtgtccga atttttcaag tgcctttaaa aagaactttt
     5941 gggtttcatt tttcggatca atataaagaa ttttttgacc taaaaacatc agccataaaa
     6001 aaatatattt tacaaggacc gattttcctt tacctggtgg accaataatc aacgtatttc
     6061 cattggtatg ttccgcgcct tcaatacttt tctttgttaa attgggataa taccatacta
     6121 gtttactaga tagcgctaac gcttctttcg cagatttaaa cttttttgca ataacaatcc
     6181 gtcctaaagg aaatcctttc ttatttccta cttctttctt taagtccaag ccataatctg
     6241 ccacaaaacc tgtcgtagcg acctgctcgt aactacgaaa acgttgcgta cttccaatta
     6301 agcattgatt aaacaacgtt agctgatccg ctaccgatcg ataaaatttg aaatctgtag
     6361 actcaatcgc aaattcaata tctttgactc gttcttccaa ttcttctttt gaagaagcag
     6421 atacaaccag cgtgacggta gaataacaca atcgtttttc tttgcgcttc agcttatctt
     6481 ttacatccat taaccgttct gtaccaaaat ttaatacatc gtctgaatct aaaatgccgt
     6541 caacggtctc atattctttt tcttgttcat aaatccgtcc agccatttta ttaatttttc
     6601 gttcatcaga ttttgaatgc tcaaatcgaa gtcgaatatg cgtatcaatt ggaaaagata
     6661 agctatcctg aagattttga ataaagctac tcccatacat ggctaaagga gcgtccgttt
     6721 gacttaaaaa cgtcgaataa tgcgtttgat tcaactgttc aatcgcaatg tatcctgctt
     6781 cattcgaaat gacgccctcg caaatttcca ttggcgtaag ctgtctttca ggcaattttt
     6841 cttctgatcg atggaaaaaa taataataaa tttgtccaat ctcttgggca gataagcgct
     6901 ctatcggctt ataattggac aattccgtat agagctcctt ttctaatgtt tcatacgttg
     6961 gtaacacact acttatagga actcgttgtc ccgtaatacg atagataaac tttttaaaaa
     7021 catgcttaag caattgaaaa tattcgatcg gatcagacac tttcatttct gtagtgaaac
     7081 gcacaaacag atacgttcga tactcgttta attgcacttc gtcttttaaa atctttcctg
     7141 cctgttcaaa gtacactttc ccaagatctg cgcattctcc ctttaccaaa tctcgaatgg
     7201 tttcttcaat gtgcgtatct aggttaaatt gacgtggaat attgataaaa tgatagtcat
     7261 attcatcatg tccaagtatg ccttcgccat cttgaagata gttcttgaaa aaatcaatgt
     7321 catttaaagg aaatacttgg gaagaaagtt gatagcctac ccacacatca tcatttttgg
     7381 tcaaaacaaa atttcggata atggcgtcta tcggaaattc taatcgagga agcttcgttt
     7441 ttttcttttt gaactgccaa atcccgataa taaagacgac caaaagaaac aaaaacgcca
     7501 tttactcgac tcactccttc catttgtgta gggaatacgt cactttttca tggaaaaacg
     7561 gcacatacat ttcatgttca aagcgttgtt tccgtttctg ataataggac gttcgcccaa
     7621 ttaaatactc gaaaaagtgt ttattgtccc atttcattga aaacaatgtc caaacgagaa
     7681 caccaatccc aaaaatcata atcaaccagg actttcgaaa cagtgtctgc aagaaactaa
     7741 tttgattcac atacgcaatc aaaaatagga tcaaaagaat gaagagaaat accccaccta
     7801 tgactaattg ttccatgcga atgccattag aaatccaggg aattttttgt cctttaatcc
     7861 cgtaaatttt gtgagggtgc ttaaactcct ttgtatagtt gtattccatg ttcactctac
     7921 ctccttttac aaaaaaagaa gcgaccagaa gttctaatct tctgatcgct tttgcagttt
     7981 gtctttttct tatgataatt gtatccaggg cacaatcatt tgcgttaccc ccacgttctc
     8041 ttcactatcc ttaataaaga taatgttctt tagccattca ccaatatcaa cggcattcga
     8101 taacaataag acgagcatta tcaaaataaa gacgccaata atggcaaacg ctgcccgcgc
     8161 aaagccttgg gtcgcataag caccaaccat tagaacaatc gcaacgattg ccaatagcca
     8221 aatgacttgt ccagatacat catcaaataa cgatttaaga tcaaaaagtt tcatttccat
     8281 tctcctctaa gttcatttta ttcatttaac gtagtaacaa aataactatc gccacttgtt
     8341 tttacttcca ttgaaaagtt tgattggaac tcgtttcctt tctcaaaata gaaacgatac
     8401 ttcccttgca aaaagtagtt tccttcttta ttttttactg catgatccag ttgtacattg
     8461 atcaattcgc cgccttctaa ccctttttcg ttagaaatca atgccaattt ttcatcattt
     8521 ttgacatata actcaaaaaa ccgtttcaca aatgcttcca gcttgatttc ttcgctttca
     8581 gtgaccgctt gcccagaaga ataaaatcga ttttcctgat agttcacaga attggtattc
     8641 ttactctcag aaagattcaa aaattgaacg ccatcaagta cttttaaaac attgttttga
     8701 taactgactg gaaaagaaag ttgactgctt ttccaatgtt ctccttctgt aaaagtaacc
     8761 tcataaaata ggcgatacat tgcttgtgtc aaatcgattt cttctagttt aataaagcga
     8821 acatctttcg ctactcgatt ggtagccgtc gttacaaagc ctagatcaga cgcttccaat
     8881 ccttgtgcta aataatgatt gacggaagcc tcccattcgt ttcgtttttc tggcgaataa
     8941 gtaaataacc tttcaataaa atgttgtccc tcgtacttca attgttccgt agaagacaag
     9001 gtttggttga cttcagttaa caatgtttct ggattgactt gttttttcac tgctaaacgc
     9061 gttaatgtat ccactcgtcc aaagtttagg aggagcacga cacccattaa cagaaacaaa
     9121 caaaaaaaag cgcctgaaaa tacactcatg gcactttttt gcgaaaattg gatcattcga
     9181 tctgacggtt ctgttttctt tttttggaac aaaaaacgtt tcttttgttt cttttccttc
     9241 tgttgtttcg gtggcttagt ttggaacgct tgtggattaa attttgcatt actcatgaga
     9301 aatgccatac cgttctaaca ataatttcgg tattttgtcc acaatcacta cttcgtggac
     9361 aaccacactt gccccctc
//
LOCUS       DAYYFU010000083         9362 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000083 DAYYFU010000000
VERSION     DAYYFU010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.088"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..761)
                     /locus_tag="ACHA34_002479"
     CDS             complement(93..761)
                     /locus_tag="ACHA34_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8793071.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            895..1182
                     /locus_tag="ACHA34_002480"
     CDS             895..1182
                     /locus_tag="ACHA34_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793072.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1598..3136
                     /locus_tag="ACHA34_002481"
     CDS             1598..3136
                     /locus_tag="ACHA34_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFM8793073.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3133..4098
                     /locus_tag="ACHA34_002482"
     CDS             3133..4098
                     /locus_tag="ACHA34_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8793074.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4085..5407
                     /locus_tag="ACHA34_002483"
     CDS             4085..5407
                     /locus_tag="ACHA34_002483"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8793075.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5470..5574
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5648..6391
                     /locus_tag="ACHA34_002484"
     CDS             5648..6391
                     /locus_tag="ACHA34_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8793076.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6406..7398
                     /locus_tag="ACHA34_002485"
     CDS             6406..7398
                     /locus_tag="ACHA34_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8793077.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7410..8147
                     /locus_tag="ACHA34_002486"
     CDS             7410..8147
                     /locus_tag="ACHA34_002486"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8793078.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8137..8859
                     /locus_tag="ACHA34_002487"
     CDS             8137..8859
                     /locus_tag="ACHA34_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8793079.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8943..>9362)
                     /locus_tag="ACHA34_002488"
     CDS             complement(8943..>9362)
                     /locus_tag="ACHA34_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8793080.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3069 a         1607 c         1972 g         2714 t
ORIGIN      
        1 atttttttat aaagaagatt ttactgaata atatcatttt agtttacata atgatatttc
       61 tgtaaattaa ataaagtagt gaaaaataag aatcagtgct ctaactgttc aagaggatca
      121 agtggttcaa atgtttctgt agtctgtttt gcttccaatt ggggcaaaac agatccaatt
      181 tccagatact tcactttatt caatgtataa gatagaatag aacagctaag taatgtatac
      241 agaaaatgtg tgagatccaa ataaagcaag gagacaacta aaataccaaa agtaatcaag
      301 aaaagacttt tgccaatctt aatatgaaaa tatttttcaa tggcttgata aatgatatct
      361 gttccgcctg tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc agaaagaata
      421 cctgtcatca aactattgat cagtagacta tctacagcaa aatgtatatg aaggctctcc
      481 cataaatgta aaaagaaaac tagcgcaaat gttccgacga tcgtccggag aaaagcgtgt
      541 tttccaaaaa gaaacaatga gataagcaat aaaggtaaat taaaaagcag tgaagtgaca
      601 gaaggagcga atccaaaaag attatgcaaa agcaaagcaa taccagtaac cccaccatct
      661 gccagcttag atggaatatt gattcctgtc acagctaatc cgtagattcc tgcgcctaaa
      721 atgattccgc acaattctgt aaataaattt aattttttca tttctagcct cttttctttt
      781 gaaaattttc agcaacttct ttattatact gacttatact cttacttcaa gaggaaaatg
      841 aaaaaaaatt atttttggct gtttcttttt atttatcaaa taaggagtgg aaaaatgcga
      901 gcagcagact tattgacaat tcttacaaaa tcacccaaaa aaacagagta tcaaggagtt
      961 tttttggctc aagaacaaaa actggtgcct gtgacaatgg tccagataaa taaagcaaat
     1021 caatttatct ttttatttga acacggcaaa gcaccacttg ctatgaaaga catctttctt
     1081 tttttgatga agaaccgtga gaaagctgtt tattatcagt caaaagaaga actcattcct
     1141 ctttatggag tgaaagagat agaaaataaa gtggtgatat aagtaaaaat aagaaaacaa
     1201 gacgtttatg atgtcaaaac aaccattttt tacatttttg gcaaaaaagt gtcatgatac
     1261 agtataatat tcgttgaaca ggccaataaa ggtgttctcc cccacgagat ttctacttta
     1321 ttggctgttt ctttttatta aaataaaagt tgtgtgtgta aaaaagatat agctgattcc
     1381 aagtgacaat ttcgtataga tttttatggt attcgtagtg aaaaaacgag aaccaaaaag
     1441 ttgatccgac tttgttgctt ggattctttt tttgtcagca tatttagagg tatttttttc
     1501 ttttttttgt tcgtgctgta aggttaaggc aagacaagtt agttgggaaa aaggaaagaa
     1561 atgtttatac ttaaagaata acagaaagga aggtcgcatg aataatttta tacaaacatt
     1621 aagtgaaaga aaaggcgagt tgctggaagc tgcatttcaa catctgtcca tttctctcat
     1681 ttctttgctg attgcagcac tgattgctat tcctttagcg atttgggcag ccaatcataa
     1741 aaaaatggca gaagtcttat tgcagatcac gggtgtgctc caaacgattc cttcacttgc
     1801 cttattaggt ttacttatcc catttgtagg gatcggaact gttccagcat tgattgcact
     1861 tgttatttac gctttgcttc cgattttcca aaacacgtat attggtctag ctgaaattga
     1921 cccgtctatt gaagaagcag cggtagcatt tggtatgtcc agaatgagac gcttgctgaa
     1981 agtagagctt cccatcgcat tacctgtcat tatctcaggt atccgaactg ccttagtttt
     2041 aatcatcggt acggctactt tggcagcatt gatcggagca ggtggattag ggacatttat
     2101 tttgttaggt attgaccgaa atacacctgt tttgacattg attggtgcga tcagttctgc
     2161 cttgcttgca attatattca gcggactgat tcgctggttg cagcataaga aacctcgata
     2221 ttccatcatt actttagcag cagtggtgtg tttatttggc ggcgtttctt tatatcagtc
     2281 tgccttgttt aaaaatgaaa ccatcacgat tgctggaaaa ttaggagcgg aacctgaaat
     2341 cctgatccaa atgtataaag aattgattga agacaacaca aaaacagaag ttgaattaaa
     2401 accgaatttt ggcaagacga gttttttgtt caatgcactg gatcaaggtc aaatcgatat
     2461 ctatcctgag ttcacaggta ctgtcctaga aagcttggtg aaggtacctg aagcaatgaa
     2521 aagtgctgat ttgacaaaag aaaaaacata tgacgaagca gaagaattat tgaataagca
     2581 attcgatatg acattattaa aaccaatggc ttaccaaaat acgtatgcct tggcagtcaa
     2641 gagatctttt gcgaacgaac atcaacttaa aaagatttcc gatttggcag ctatctctga
     2701 tcagatcaaa gcgggattca cactggaatt tattgatcgt tcagatggat ataaaggaat
     2761 ccaagaagca tatggcttga atttttcaag tgtacaaagc atggagccaa gtttgcgtta
     2821 ccaagcaatc aataatggag atgtcaatat tgtggatgcc tattccacag atagccagct
     2881 caaggagtac gatctagtga ctctagaaga tgatcgaggg ttattcccag cttatcaagg
     2941 cgcaccgctg atgaaaaaag attttgctaa agaacatcct gaaatcgtta aagcactgaa
     3001 taaattaagc ggaaaaataa cggaggatca aatgattgat atgaattacc aagtcaatgt
     3061 cgaaaagaaa aagccgacag aagtagcacg aatcttcctg aaaaatgaag gattagtaaa
     3121 ggaggaagat cagtgactaa attaatcgag ttcaaacatg tgcaaaaaaa gtatgatgga
     3181 aagtatgtca tcgatgacct aaatctggca atcaaaaaag gagaaatctt tgttttggtt
     3241 ggtccttccg gtagtggaaa aacaactact ttgaaaatga tcaatggatt atctaaacca
     3301 tcagcaggag acatctactt caaaggaaaa tcgttaaatg aatataatct gcagaaaatg
     3361 cggtggaata tgggatatgt gctgcagcaa atcgctttgt tcccgaccat gacagtcaag
     3421 caaaatattg aagtcatccc tgaaatgtta ggttgggaaa agcagaaaag agcagaccgt
     3481 gtagatgaac ttcttcaaaa agttggctta tctcctgata tttatcgaga tcgtatgcct
     3541 agagagttgt ctggaggaga acagcaacgg atcgggatca tccgagctat tgctgcaagc
     3601 ccagatgtga ttttgatgga cgagccattt agcgcattag atccgatttc aagaaactca
     3661 ttacaagaat tggttttgtc cttacacgaa gaattgggca cgaccatcgt tttcgttacg
     3721 cacaatatgg aagaagcaat caagctaggt gatcgtattg catttatgaa ggatggagaa
     3781 attatccaat gtgacacacc agagcagtta ttgatgaatc ctaaaaacga ctacgttcgg
     3841 catttctttg atgaaccgaa acagacgaaa gagtggcgag tagaagattt agtcgtcaat
     3901 ggatatttct taaatgaaat accagaaaat gctcgtcaac aagtttgctt tgatacgcct
     3961 atgaaagaag tatattcgct tctttcggtg tatccaagca ttgtgatagt agaaaagcaa
     4021 agaagtattg ggtcgctgac aagtaaagaa atctttgctt ttttaagtcg agaggaggaa
     4081 ggaaatgaaa acatatgatg caatcatcat cggttcaggt gtgagcggct taagtgctgc
     4141 ttatggattg aaagaagcag gtaaaacagt actagtggtc gaagaagatc tatggggcgg
     4201 gacttgtcct aaccgtggat gtgatcctaa aaaagtcttg ttaagtgcag tagaggcacg
     4261 taatcgggta aaacaattga gtggaaaagg ttttaacgag atcccaactg ccaactggga
     4321 agaacttcaa aaattcaaac gaacatttac ggatccagtc ccagaaagcc gaaaaaaaca
     4381 attagcagaa gctgaaatcg atcatctttc aggaaccgct cgttttttgg atgattcatc
     4441 tattgaagtc aacgaagaag tcttccatgc ggattactta gttttggcaa ccgggcaaag
     4501 acctacgatc ttaccagtgg agggaaagga atacttgaaa actagtgcag actttttatc
     4561 attgcctgta ctgcctaaag aaatcatttt tatcggtggt ggttatattg cttttgagct
     4621 agctacgatt gccaatgctg cagggagcaa agtaaccatt gtccatcata atcaacggcc
     4681 attaaaagaa tttgaggcgt ctttggtaga ggaagccgtc catcaaatgg aagcatctgg
     4741 cattcagttt gcttttggtg ttgaaaccca aaagatcatt tcagaaggta ctcgctatcg
     4801 tttagtcgga aaagagacgg aattagtagc agatatgatt ttttgtgcga ctgggagaca
     4861 gccgaataca gaatctttag cacttgagca agcgaatatc gtctttgata aacacggaat
     4921 tgcagtcaat gattatctgc aaacgagcaa tccaaaaatt tttgcgtgtg gagatattgt
     4981 ttcaaggaaa accccaaaat tgacaccagt cgccactttt gaaggaaatt atgtagctaa
     5041 gcggataacg gatgcaacaa gcgaacctat caaatatccg attataccta cgatcgtcta
     5101 cgccagtcct aaacttgctg aagtaggagt tacaaaaagt cacgcctctt cgtcagatca
     5161 agtggtagag atggatctta ccagttggtt cacctatcat cgtgtcaatg aaccagttgc
     5221 taaggctgaa ttgacctttg accagcaaaa ttatttgatt ggtgctgcag tgattagcga
     5281 gcaagcagac gaattgatcg atgatttgac actcgtcatc aatcaaaaat tgactaaaaa
     5341 agagttggat agctatatca tggggtatcc gacattggca agtgatctct cctatttatt
     5401 gaaataatca gcatgataac taaagcgctt gttgcttttt ccttcttgtg ctacacttta
     5461 aaaaatgaat acctgtttct ggggatgtcg tagacgacat gagagtaagt tgtgaactta
     5521 gacccattga acctgtcagt taatactggt gaagggagaa acatagcaga cgcttgaagc
     5581 gggttactat gtcgtgccca tttcaagcgt tttttgttat tcatattaga agaaaggagg
     5641 taacaaaatg aagaaaaatc tttatccagc agtcagtatg gcattcattt taattatttg
     5701 gcagattgtt gtcatagttt ggaaaatacc tgcctttatt ttacccggac ctgctgctgt
     5761 attttctgca ttagcggcag atgcacaagt attactttcc cattctgtca cgacattaaa
     5821 tgaagcagtg cttggtttgg taatagctag cgttctttct tttattactg ccttggcaat
     5881 ggattattgg aagtttttag aaatgtcttt ttatccgtta ctagtgattt cacaaatgtt
     5941 gccaatcatg gtgttagggc cattacttac cctatggttt ggtttcggta tgcttccaaa
     6001 agtgatcctt gttgtcttga taagctattt tccgatcgta gtttcttttt cggatacatt
     6061 gaggaaagta tcaaaagagc aaatcatctt tttgaaaacg atgggggcag atacagggaa
     6121 gatttatcga atatataaaa taccagcggg tttatccggc ttcttttccg gtttaaaagt
     6181 ggctgctact tattgtgtca gcggagcagt cgttggagaa tggctaagcg ctcaagcagg
     6241 cttagggtat tacatgatac gcgtaaaaaa cagctaccag atcgataaag tttttgctgc
     6301 gatcatttgt gtcattctat tgagtttatt gctgaatggc gcttgttcat tattgaaaaa
     6361 agggtattat cgtatgcttt atatttttta agagaagggg atcaaatgaa aaaattatgg
     6421 cttttattac cgctactgtt gcttttatcc gcttgtggca ctgcaaaaga aacaagtagc
     6481 aaacaagaaa taaaggattt gaaaaaagtg acacttgttt tggattatgt accgaatact
     6541 aatcatactg gcatctacct agcaaaagaa aaaggctatt ataaagaggc tggtttaaat
     6601 gtccaaatca tcgaaccagg agataattcg acaagtattg gtctagtagg tgcggacaaa
     6661 gcacaatttg gtgtaagtta tcaagaagat gtcacttatg ctcacgcaga tggacagaat
     6721 ataccagtca aagcaattgc tacagtgatc aaacataata cttctggatt cgccacccta
     6781 tcagacagta acatccattc accaaaagat tttgaaggaa aaacatatgc aggttggcaa
     6841 agtccaagtg aagaagctgt cttaaaagct gtgatggaaa aagacggcgg ggacttttca
     6901 aaactgacaa tggtaggaag caatggagaa gggccagaaa gcttaggaaa atcctcagat
     6961 atccaatggt actttgaagg ttgggatatg atcaaagcaa aagaagcggg tattgaacta
     7021 aactatatcc cattgaaaga attagatgaa cggttagatt actatactcc ggtaattatt
     7081 acgaatgatc aactgatcaa aagtgatcca gaattggtac agtcttttat ggatgccacg
     7141 aaaaaaggct atcaggaagc tattaaggat ccaaatgaca gtgctaaact gttgcaaaaa
     7201 tatgcaaaag aaaatgatcg gacgttttta gaagaatcac aagcattctt gtcaaaaaat
     7261 tatacggatg atcctgagaa ttggggattg atggaagaaa aagtttggcg caattataca
     7321 gcttttatgc aggaaaacgg attgatcaag caagatgtac cttcgcaaga actattcact
     7381 aatcagttta taaaatagga gtgatagata tgtcaatcca aatcagccac ttgaatgtag
     7441 tcattgaaaa cctgaaaata ttagaagaca tccatttcac tatccctaaa gataccttta
     7501 catcgattgt cgcgccaagc ggagccggta agtcaacatt gctgaaaact ttaacaggag
     7561 tccagcctat gacttctgga acgatcaaag tagacgatca aaaagtaact ggtctccata
     7621 cagcatttag ttatatgccg caagaagata tgttacttcc ttggctgagc gtctatcaaa
     7681 acgttacgtt atatcaaaaa atcaatcatc taacaatcaa tgaagaacaa gtaagggatt
     7741 atctggatat ctttgggtta aacggctatg aacatttttt gccggaacaa ttatctggcg
     7801 ggatgaagca gcgaacggcc ttgttacgaa caatcatgaa tccttcttct tacttgcttc
     7861 ttgatgaacc atttggagcg ttagatgcta tgaccagagg gcagatgcaa gactggatgt
     7921 tgaagctgcc taaaaaagca aagcggacta ctttacttgt gacgcatgat atcgaagaag
     7981 caatttattt atcagacagg attcttgtgc tttccgcccg tccggcacat gtgattgctg
     8041 agattcaagt gcctgaaaaa cgacggtcac gtgaatggct actgcagcaa tcagagttga
     8101 agcaaacgat ttatcagtta ttggcaggtg aattagatgt taagtgatgc tcattgtcat
     8161 ttagatggga gtcagagttt agcactcctt cagcaggaac acacgatttt gacgatcatc
     8221 aattgtgatt cgccagaaga atggaaggaa aacagacagc ttgcagcaag taagactcaa
     8281 gccttaagtt acggtatcca tccttggaaa gcagatagct atacgtttga acaagttgag
     8341 ccttttttga aaaaagcaag gatcatcggt gaaatagggt tagataatat ttggacgaat
     8401 gtaccgatga ctacacaaaa aaaagtattt gagcgacagc ttgcttttgc agccataaat
     8461 gagaaacctg ttgtactgca cacaaaagga tgcgaaaaag agatcttaaa ttatatccaa
     8521 gcttacccta atcactattt gatccattgg tattcttctt tggactatca acaagactat
     8581 attgatttag gctgttattt cagcattggt cttgatttga aaaagaatcc agcagtctgg
     8641 caactagcta aagcagtccc aatcgaccac ttacttattg aaacggatgg actacatgca
     8701 gcagagtggg ctttaggggg gattttttca gaaaaagatt atgtctcact gcttgaagaa
     8761 cagttaaaga caatcgctga gatcaaagag ctatcagttg aagaagttaa aaaaaatact
     8821 tatcaaaact tggaacgttt cattagatta ggtaaataaa aaagagatgt gacacaacta
     8881 gtgtcgcacc tctttttggc tttctttgta taaaagtaaa ctgtttgatt aataaatgaa
     8941 tcttagtacc aaccgtttgc ttcccagaat gctttcgccg cttcccaaga accataacgt
     9001 gaagtaacat attgttctgc tactctttct tggtttgcag cagagtagtc accatttaag
     9061 taagaagaat ctaattggta acgtccgatg taacgtccgt tcgttgctgt gtaagaaccg
     9121 cttgattctt tttgtgcgat ccattctttt gcagagcttg tgcttgctgg agtagcagca
     9181 gtttctgtta cagcaggagc aggttgtgtt tcagcaacag gcgctgcttg tttttccgtt
     9241 tgaaccgttt cttgtacaac aggttgttca gcttggactg gtgcttcttg aacggctgct
     9301 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
     9361 ta
//
LOCUS       DAYYFU010000084         9357 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000084 DAYYFU010000000
VERSION     DAYYFU010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.089"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACHA34_002489"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACHA34_002489"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFM8793081.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACHA34_002490"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACHA34_002490"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFM8793082.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACHA34_002491"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACHA34_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFM8793083.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACHA34_002492"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACHA34_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFM8793084.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACHA34_002493"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACHA34_002493"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFM8793085.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACHA34_002494"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACHA34_002494"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFM8793086.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACHA34_002495"
     CDS             complement(8053..8523)
                     /locus_tag="ACHA34_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFM8793087.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACHA34_002496"
     CDS             complement(8569..8994)
                     /locus_tag="ACHA34_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFM8793088.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACHA34_002497"
     CDS             complement(9061..9330)
                     /locus_tag="ACHA34_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFM8793089.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
BASE COUNT         2355 a         2095 c         1621 g         3286 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttt
//
LOCUS       DAYYFU010000085         9335 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000085 DAYYFU010000000
VERSION     DAYYFU010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.090"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..271
                     /locus_tag="ACHA34_002498"
     CDS             <1..271
                     /locus_tag="ACHA34_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793090.1"
                     /translation="MSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVT
                     ATATYDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            317..1261
                     /locus_tag="ACHA34_002499"
     CDS             317..1261
                     /locus_tag="ACHA34_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8793091.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1277..2056
                     /locus_tag="ACHA34_002500"
     CDS             1277..2056
                     /locus_tag="ACHA34_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFM8793092.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            2068..2766
                     /locus_tag="ACHA34_002501"
     CDS             2068..2766
                     /locus_tag="ACHA34_002501"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFM8793093.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            2794..3558
                     /locus_tag="ACHA34_002502"
     CDS             2794..3558
                     /locus_tag="ACHA34_002502"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFM8793094.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            3602..5053
                     /locus_tag="ACHA34_002503"
     CDS             3602..5053
                     /locus_tag="ACHA34_002503"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8793095.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            5085..6221
                     /locus_tag="ACHA34_002504"
     CDS             5085..6221
                     /locus_tag="ACHA34_002504"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8793096.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            6221..7327
                     /locus_tag="ACHA34_002505"
     CDS             6221..7327
                     /locus_tag="ACHA34_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM8793097.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            7333..8355
                     /locus_tag="ACHA34_002506"
     CDS             7333..8355
                     /locus_tag="ACHA34_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HFM8793098.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            8377..>9335
                     /locus_tag="ACHA34_002507"
     CDS             8377..>9335
                     /locus_tag="ACHA34_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8793099.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHK"
BASE COUNT         3405 a         1315 c         1950 g         2665 t
ORIGIN      
        1 tatgagcttt tttaaagata gtccttggaa aacggttgtt atgcaatatc taggattcgc
       61 tgatattcag atcgcgcaag tcacgcaact agatcagtta ttcgatacga tgttaaagga
      121 tggtcaagtg actgccaccg ctacttacga tcaatatttg acggtgctca gtcaaataga
      181 gcagatccgg aatgaaaaaa taagcgcagc atacgcaaca aaagcacaaa ctgttgctga
      241 gcaaatgggc tatagcaata taagctattg acagttaaaa cgttgattca agcaaggtta
      301 gcaaacggag gaaactatgg caagacgaag aagaaaatca agagggaaaa aaatatttct
      361 gattatttta gcagttctta tcgtattagt attagcaatt gtaggagtag gagcaaaatt
      421 atatctggat ctctcacatt ctattgagaa aacttacgaa tcagtggaac gaacccaagg
      481 agaacgaacg aaaccagttg atctggacaa acaagaatct ttctctgttc tattgatggg
      541 gattgatact ggagatttag gtcgggtcga tcaaggaaga tcagatacga tgatggttgc
      601 cacagtcagt cctgaagaca aacagacaac gatcgtcagc attcctcgtg atacgtatgt
      661 cgacatcgta ggtcgtgaca caaaagacaa gatcaaccac gcttacgcat tcggcggagc
      721 tgccatggcg atggataccg tacaaaaata tgtagacatc ccaatcgatc actatgtctc
      781 tatcaatatg aaagggctaa aggaactcgt tgatgcagtt ggtggtatcg aagtcaataa
      841 tgacctcact ttctcacaag atggctatga ctttccaatc ggtaaaatta cattgaacgg
      901 ggatcaggca ttatcttatt ctcgtatgcg ttatgaagac ccaaatggtg attacggtag
      961 acaagggcgc caaagaaaga tcgtagaagg cgtagcgaaa aaagtattga gtctaaacgg
     1021 tgtcagcaac tatcaatccg ttttgaatgc tctagagtcg aatatgaaaa cagacatgaa
     1081 ctttgatgaa atgaaaaaaa ttgctttaga ttatcgggag gcatttggta cgattaagca
     1141 agatcagctg caaggagaag gcttcatgca agataatatc tcctatcaaa aagtcagtga
     1201 tcaagaactt gatcgtgtga aaaaagaatt aaaagatcaa atgaatctag agaataaata
     1261 ggaaagtgga gaaaaaatgg aagagacaat cagcttgaaa gaaatttttg aaacattacg
     1321 caaacacttg gtaataatca tcatctcaat gtttgctggc ttggccatct caggaatcgt
     1381 aacatttttc atcttgacac caaaatacag ttcacaagca caattgatcg tgacactgcc
     1441 acaatcagac actgcaaatg tcaatgatgt caatacgaac ctgcaaatga tcaatacgta
     1501 taaagatatg atcgtcagtg atttagtgct caatgaagtc aaagatcgtt tagaaacaga
     1561 agacaatgtg aaaatgactg ctgaacaaat caaagatgcc atctcagtta atcaggcaga
     1621 aaattcacaa atgttctcga ttcaggcaat aagtacgaat gcagtggctg ctcaacagat
     1681 cgctaacaca acttcacaag ttttccaaga gaacgcaaaa gatgtgatga atgttgataa
     1741 aatctcgatc atttcaggtg cagttgccaa tacgacacct gtttcgccaa ataataaatt
     1801 gaatttaatg actggcttaa tgttaggaat gatggtaggt gtaggtctgg cattcttatt
     1861 ggaattactc gatcggacag tgaaagatga aaaatatgtg acagatacat taggattccc
     1921 aatcttagga acagttccag aaatgtcagc gaaagaattg aatgctggta tccgtaaaaa
     1981 aggaccagct cagacacaac caaaagctac aaacgataaa acagcgccat cacgcagaag
     2041 ccgctcaaga gtctaggagg aaagaaaatg gcacgaacac agaaacaaaa agcagcccaa
     2101 actcatgcag tcagtttggt cacactcacc aatccctctt caccaatcgc tgaacaatat
     2161 cgtacgattc ggacgaatat ccagtttgcc tccgcagctg gtcagcaaat caaaacgatt
     2221 gttgtgactt catcaggacc tggagaaggg aaatcgacca ctgcagcaaa cattgccgtt
     2281 gtttttgcca aatctggtca acgggtgctg ctagtcgatg cagacttacg taagcccgtc
     2341 atctataaaa cgttcaagtt gaacaacgca agcggattaa gtactgttct tagttcatca
     2401 ggaagtgtag cagatgagat ccaacgaaca ccagtcgaca atttgtcgat tctgccaagt
     2461 ggacctaaac caccaaatcc atcagaacta ctaagttcgc caagaatgga tcagatttta
     2521 gcagaagcaa gacaactatt tgatgtagtg atcttcgaca tgccgccagt cgttgccgta
     2581 acagatgcac agatcatgtc ttcaaaaacg gatggcacct tattggtcgt acgggaaaat
     2641 acgtcacgaa aagaatcatt gactaaagca aaagaactgt tagatatggt acaagcacga
     2701 gtcttaggtg tcgtatacaa cggagcagaa cacagcaaag atgctggtta ttactacgga
     2761 aattaaggaa taagaagaga ggagacaaga gaaatgatcg atctacattg ccatatccta
     2821 ccaggaatcg atgacggggc agaaaactta gaagcaagta ttgcaatggc tgaaaaagcg
     2881 attcagcaag gcattacgca tattttatgc acaccacacc acaataatgg aaaatacagc
     2941 aatgaaaaat cacaagtcat ttctctagtc gcttcgttac aagccgaatt agagaaaaga
     3001 cagttgcctt tgacgttatt ggaaggacaa gaagtacgta ttaccggaac gctcatcgaa
     3061 gacatccacc gagatgaaat tttatttaca gatttagatg atacatatct tctgatcgaa
     3121 tttccgacac tggaagtgcc gttatatgca gaacggcttt tttttgagtt gttagaacta
     3181 ggaaagatac cagtgatcgt ccatccagaa cgcaacgcgc attttcgaga agacccaaat
     3241 catttgcttc cgttcctaga tatgggatgt ttggcccaac ttactgcgcc aagttatgtt
     3301 ggtgtatttg gcaaagatat ccagaaaaca gccaaaacaa tggtcaaaca taatctcgtg
     3361 cagatggctg cttctgatgc acacggggtc tctaagcgca ccttttactt aaaagagtgc
     3421 tatgagcaga ttgccaagga gtttggtaag gaaaaagtag tacagatgaa acaagtcgca
     3481 aaggatctga tcaacgggga tgagatcaat tatccaatct atgaagaggt aaaaaagaag
     3541 aagtttggtt tattttagtt ctttattcgt agataaaaag gaaatgagga gagagtgaga
     3601 gatgtataga aaaaactcaa gtggttggtc taaacatatt gattttattt tattagactt
     3661 attttgtgtc cagcttgctt tttatatatc atatgtaatg cgacagggtg actggaatcc
     3721 gtatgtgatt cctatttatc gccacatggc aatttttata gaacttgaga atcttacgat
     3781 tatattttta tttgaggcat ataaaaatgt tttaaaaaga ggatattata aagaggctgt
     3841 agcttctata aagcaatctt ttatgctgtt acttgtgtgc agtttatacc ttattacaat
     3901 gcaggatgga aatgattatt ccagagtttc tttatttata atgggggtgg tgtatggatt
     3961 attaacatat ataattcgta tactttggaa agggttgttg catcaccgca tgaaaaacgg
     4021 tgaagatgta tcgttagtta ttgtaacatc tcaaaagatt gcaaaaaatg tagttcgtaa
     4081 tcttaaagag aagaattatg gcatgtataa acttagtggt gtgattgtag tagacagaga
     4141 tatgtatgga actaccatcg gaggtgtccc agtagttgca aatgctgata atgcagcgga
     4201 atatctttta cagaattggg tagatgaagt attcatcaat gttgacgaaa gtgttccata
     4261 tcccaaagaa ttaatcgcca gatgttcgga aatgggactt gctgtacata tgaatcttac
     4321 aaaagtgtct gatagtacac gtggaaaaca atttattggc cgagttggtg attatactgt
     4381 tattacatct agtattaatg ttatgacgat gaggcaagca tttataaaga gaacgattga
     4441 cattcttgct ggaattattg gatgtattgc aacaggtgtt atttttatct tcgtagcacc
     4501 agttatttac atcaaatcac cagggccgat tttctttgca caagaacgta ttggtcaaaa
     4561 tggtaagaca tttaagatgt ataagttccg tagtatgtat atggatgcag aagaacgcaa
     4621 agcagagttg atgaaagaaa ataagatgag taataatctc atgtttaaga tggattttga
     4681 cccaagaatc attggcaata aaatacttcc tgatggaaca aagaagacag gaattggaca
     4741 atttattcga tctacatcat tggatgagtt tccacaattt tataatgtcc taatgggaaa
     4801 aatgagcctg gtggggttta gaccttgctt gaagtcagaa tatgagaagt atgattttca
     4861 tcatcgtgcg agaattgcga tgaaacctgg gattactgga atgtggcagg tgagtggtag
     4921 aagtgatatc acagacttcg aagaggttgt aaaacttgat gcagaatata tcaggaattg
     4981 gagtatggga ttagatttta gaattttgtt taggacagtc aaaactgtgc ttcatagaaa
     5041 cggttccatg taagataaag atactataga aggagtaaaa cgtgatggca agaaaaaaat
     5101 taaaaatctg tatgctaggt cataaaagaa ttccatcaag agaaggtgga attgagatag
     5161 tggtagaaga attggcaacg cgaatggttg cacttgggca tgaagtgact tgctataatc
     5221 gaagtggtca tcatgttagc ggaaaggaat ttgaccagca taaaaataaa gaatataaag
     5281 gtgtaaaatt aaagactatt tttacattaa atataaaagg aattgcagca atgagttctt
     5341 cagtatttgg tggaattaga gcagcattcg gaaaatatga tgttgttcat tttcatgcag
     5401 aaggaccatg tgctatgttg tggttgccaa aactttttgg gaaaagatgt gttgcaacaa
     5461 ttcatggtct agatcatcaa agagaaaaat ggaacaagct tgctagtaca tacatcatgt
     5521 taggggaaaa gtgtgctgta aaatttgcag atgaaataat tgtacttagt gaaagcgtcc
     5581 aaaattattt tgaagatata tatggaagaa aaacaaggtt tataccaaat ggcgttaaaa
     5641 aaattgaaat aaaatcagca ggattgatta cagaaaagta tggattaaca aaagattctt
     5701 atattttgtt tttggggcga ttggttcccg aaaaaggaat tcggtatcta attgaggcat
     5761 ttaaagatgt tcagacagat aaaaaactta taatagccgg aggaagctct gatacagatg
     5821 aatttgcaaa tgaactaaag gaattagcaa aaggtgatga gcgaattata tttactggat
     5881 ttgtacaagg acaggaatta gaagaattat atagtaatgc gtatatctat acacttccaa
     5941 gtgatttaga aggaatgcca ttaagtttgt tagaagcaat gagttatggg aactgttgta
     6001 tagtttcaaa tatctcagag tgtacggaag tagtagaaga caaggctatg atttttaaga
     6061 aatcggatgt aagtgattta aagataagat tagaagaggc atgcaatcaa tcggaaatgg
     6121 ttaaagtttt gaagaatcaa gctactgagt ttatttgtag taaatataat tgggataaaa
     6181 tcgtacaaga aacattaaat ttgtataggg gaaatatata atgattcgaa tattacagtg
     6241 tgtgaatgat atgcatcgtg cagggttgga gacgatgcta atgaactatt ataggaatat
     6301 agatcgaact aaaattcagt ttgatttttt gacacatcgt ccatataaaa gtgattatga
     6361 tgaggaaata ataagtcttg gaggaaaggt ctattatgca ccgagacttt atccacaaaa
     6421 ttatcctaaa tattttaaat ggatggaaca attttttaag gatcatccag aatatgagat
     6481 tgttcattct catattgatg cgatgagtta tttaccttta aaagctgcaa aaaaagctgg
     6541 gattccgata cggatagcac atagccataa tacatcgata gatagagatt ttaaatatct
     6601 tttaaagcaa tattatcgat gtaagttaac gtctgttttg acacatgaat tttcatgtgg
     6661 aaaagtagca ggtgattttt tgtttcgaaa tgataaattc actttgattc caaatgcagt
     6721 ggatgctaaa aaattttatt atgatgaaag tattagaaat gatgtacgaa tgaaattagg
     6781 aatttcaagt cagacgtttg taatgggaca tattggaaga atttcatatt ctaaaaatca
     6841 tcgttttttg attgaaattt ttaaagaatt tcacgctatt aagaataatt ccgtattatt
     6901 aattattgga actggtgata tggaagaaga aataaaaaat tatgcaaaga aaagtggcat
     6961 agatgatgat attaaatttt taggaaatag aaatgatata gaaaaatttt atcaagcatt
     7021 tgacgtatta atgttgccat cattatttga aggggttccg ttagtgggta ttgaagctca
     7081 atttgcagat ttaccgtgtt tcttttcgga gaaagttcct acagaagttg ctttcagtga
     7141 taagaccaat ttcataagtt taaatcaatc ggcaaaagaa tgggcgagag agattgcaaa
     7201 tgtagatatc tctcatcgag attcagaacg ttcgatttta ataaaggcag attataatat
     7261 tcaaacagca tacaaaatct tagaatcaaa atattatgag ttatttgaat taatacaaag
     7321 aggttaaaga atatgacaca gaaacattca agtataaaaa gaattataca tcgcatatcc
     7381 gaagaattga atgcaacaag acgaattgca aagataacat cttggaagga agcatttgcc
     7441 acgttacaag ctaaaattga tattcaagtc atgaatcgta atggatataa tgaaagtaaa
     7501 aaacagaagg aacatttatt aaaaaaacat gaggtaatgc tgaagtatta tgaaaagact
     7561 ttttcagatt ttttgaaaac atatgattat agtcaaaatc aaataattaa tcaatcaaaa
     7621 tcaccatatt ctaactgcat ttgggtttgt tggtggcagg gattggatca atcgccggaa
     7681 attgtaaaag cttgtgtaaa atcaataaaa cgaaatgcag ggaaacatac tgttataatt
     7741 ttaacagaag ataattatag acaatatgtt gatataccaa aatgggtgga agaaaagaaa
     7801 aacaaaggaa tcattacgag aacaaattat tctgatttat taagattatc tttgctagca
     7861 aaacatggag gtttgtggtt agacgccaca ttttattgta caaaaccaat accagatgag
     7921 tattttgata tcgatatttg gtctattagg aggcctgatt atgctcacgc atccgtagca
     7981 tcaggatatt ttgcgggtta ttcattagca tgtaatcaag agtctagatg gatgtttgct
     8041 ataattagag actttttttt aaattattgg aaaaataatg atatgatggt ggattatttg
     8101 atgatagatt atatgattgt tttagcacag agaataaatc atgaaatagc acagacattt
     8161 gataaaatac ctagtaacaa tccattatgt gacgaattga ttaaaaattt aaataatata
     8221 tatgatgaaa aagtatggaa cttattaaag aaagatactt tactgtttaa gttgacatgg
     8281 aaacaatctt ttaaaaagca aaaagatgat aaagttactt tttatgggat gttggtagaa
     8341 aacaaattat tataaatatt agaggtataa aaagttatga aacacaaggt aagtgtgata
     8401 gtaccaattt acaatgttga aaaatattta agaaaatgtg tggatagttt attgagtcag
     8461 acattaaaag agatagaaat cgtattagtt gatgatggtt cgccagatgc ttcgggggaa
     8521 atagcagatg agtatcaaaa aaaatattct aatgtaaaaa caatacatag agaaaatggg
     8581 gggttaggac cggcaagaaa tacaggaatt gagaatgcga caggagaata tgtggctttt
     8641 cttgattcag atgattgggt agaaagtgat atgtatgaaa aattatactt agttgcgtcc
     8701 aaagaaaatg ctgatatagt agttagtgga cattgtgatt ttgcgaatga caaagctatg
     8761 gtgaaaaagc aacatccttt gagtgggaat gtatatgata cgcctaatag tattaaaaat
     8821 gtacgaaaaa atttatttgg acatagtcca aaagatcaag aagtagaggc cttcccgatg
     8881 tcagtatgta tgtctttgta taataaaaat ctgctagata aatatagact aagatttgaa
     8941 aaaattttat ctgaagatac aatttttaac ttgaatgcat atgatgtcgc aaataaaatt
     9001 gcttttacag aatatacaga ttattgttat agaaaagaag aacaagagtc tattacaaaa
     9061 agtttatctc caaataagca gacggaattt aaagaatttt taataactct aaaagctata
     9121 gcatcgaaag aagatttaga atgtcaaatc agagcaaaac gtatggcaat tgattattgt
     9181 aggctttatg tgggcttagt tgatgatgca aatttgggaa ttaaagagaa aagaaagtat
     9241 ataaaaaaat ttattgaaga tgagggatta attcaatgtt ggaagggata tcctacaaat
     9301 acgctaccat ttcaacaaaa attttttcat aaaat
//
LOCUS       DAYYFU010000086         9332 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000086 DAYYFU010000000
VERSION     DAYYFU010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.091"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            519..1754
                     /locus_tag="ACHA34_002508"
     CDS             519..1754
                     /locus_tag="ACHA34_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793100.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMI
                     FLLVPMIVSWVDNQQRISE"
     gene            1838..2926
                     /locus_tag="ACHA34_002509"
     CDS             1838..2926
                     /locus_tag="ACHA34_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HFM8793101.1"
                     /translation="MKKILVLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEI
                     AKKIEEKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFD
                     EFICFSMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLK
                     KRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKY
                     IFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSS
                     IPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAY
                     DIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            2923..4344
                     /locus_tag="ACHA34_002510"
     CDS             2923..4344
                     /locus_tag="ACHA34_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8793102.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINIS
                     YIKQLCYRGRNIISMIKSREHKR"
     gene            4380..5096
                     /locus_tag="ACHA34_002511"
     CDS             4380..5096
                     /locus_tag="ACHA34_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8793103.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            5115..6698
                     /locus_tag="ACHA34_002512"
     CDS             5115..6698
                     /locus_tag="ACHA34_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HFM8793104.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            6827..7411
                     /locus_tag="ACHA34_002513"
                     /pseudo
     CDS             6827..7411
                     /locus_tag="ACHA34_002513"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            7357..7875
                     /locus_tag="ACHA34_002514"
     CDS             7357..7875
                     /locus_tag="ACHA34_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793105.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            7898..8923
                     /locus_tag="ACHA34_002515"
     CDS             7898..8923
                     /locus_tag="ACHA34_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM8793106.1"
                     /translation="MKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVAVSLAI
                     FGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQRPSKVI
                     YDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAKKRGIT
                     ISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINSGELNK
                     DGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYNIQIVD
                     RVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYNLMNSG
                     GNGRVLR"
     gene            9172..>9332
                     /locus_tag="ACHA34_002516"
     CDS             9172..>9332
                     /locus_tag="ACHA34_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8793107.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         3379 a         1035 c         1734 g         3184 t
ORIGIN      
        1 ccttccaaca gtatataaaa tgagcgtaaa aacggctatt tttgccttaa caacgatttt
       61 tttgtataca aatacgatat gctcattaat tattggaatt tgtagtgctg tttaaattaa
      121 tgaaggtgta attatatgag tgatcatttt gaaaaagttt gttgagaaga tgtgcttcta
      181 gtcatgtgat gggagaaaat aatttaactg ttagagtgaa tagttaaatt attctggtta
      241 tatgaaaaaa gtagtttcta aaataatctt gcagggatta acttaaagaa attgagagaa
      301 tagtttcttt gaaatttcta tatacaatga gaagctgaaa tttactttta tttgatgact
      361 ggaaatagaa attaaatata ttgtattgtt acattaaaga aaacttgtat ggaaaaatca
      421 gtacaatacc attatttaat gttcttcact taaagcgcaa aaaatatcag tttattaaac
      481 tcgtatatag aacaaatata tgtagacggg gcgaaacgat gagtgtaaaa aaaatgctat
      541 ttatggtaag tacaataatt gtattaacat cccgattttg gggaattaat actagtttat
      601 ctatgcgata ttttattatg gcaatatggg atttatattt tatgttaagc tatttttctt
      661 atcgtgagaa aacatataaa aaatacgtta tcaagaataa cttttggttg actataatgc
      721 cgtttgtgat attttctatc tatactctaa taatatgggg aattggttca aatgcagaat
      781 ttgaaaattt tataaagtta tgttccacgt tgctttattt aattcttgca tatggttacg
      841 catgtacagc attttactta tttaaggcag aaggaattga ttttattttt tggtcaggtg
      901 ttatttcata tatgagtggt tcagtttttt atttaattgt atcttatggt ttaaatggtt
      961 taatttctta tataaaaagt cttataacag gtgaaacaaa tactgcaaat tttgctatgg
     1021 aggtacatga tttaactttt gcgatggggg tttttttcct ttattacgta ttttttgaaa
     1081 ataaaaatac aaaaaaacat aagataaaaa ttatcgtatc tttaatttta attttcatgg
     1141 gattgaagag aattgaactg ttagctttaa gtggtgcaat tctagtgtat tatgttttat
     1201 taaagtgggg aaaaaagatt caaatccgtg cattagtgtg tactatttgt gcaactgtta
     1261 tttcgttagt gtttatttac attatagata caggaatttt ggcattgctt atgtcaaatt
     1321 tgggcattac agatgacggt gcgaggcttt cattttatct atattcatct aaattttatg
     1381 aacttggttt aggatatgta ggaacaggat ggacatggtt ttcaaaatac ttttttaatc
     1441 tttatttatc gcactttagg ttaaatggtg gaagaattgc agcaagccta cacagtgata
     1501 tattaactat ttatatagaa gttggatgta tagtttttat tatatggatg ttttggttat
     1561 tttttgtgaa accgataaga ctaactaaaa aatttggatt gaaaacaggg gagtgtgtat
     1621 tattattaac tatatatatg tttatattat atttgacgga taatacgctc acttatccag
     1681 acactcaaat gatttttctc ttagtaccta tgatagtttc gtgggttgat aatcaacaac
     1741 gaatatcgga atagatagct aaagtatatt ttaattatga atttataatt agttgtaaaa
     1801 gttaatattt aacggacagg aagtagggtg aaaaaatttg aaaaaaattt tagtgttagc
     1861 aaatacatat tatcaaataa tagtagcaat acaaatgaaa aatacaattt tttccgatga
     1921 gtatgttgaa attattattt ctgatcattc taaaaacact aaagaaattg cgaaaaaaat
     1981 agaagaaaaa aaagtgtttg atggctgtca ttatttagaa agcaaatctt ttgatgataa
     2041 tattgggtat tttcaaaaga ttaaggactg tcttcagatt tcttgtggta aatcaaatcg
     2101 atatgcctat tatttgaaaa actgtaaaag tgaatatttt gatgaattta tttgtttttc
     2161 aatgggagag gtttggttgg aaggaatata tgcttgctta tatcgaaata ataaaaatgt
     2221 acaggtatca attttagaag aaggtatttt aagctatgga gttgacatta ttccaacagc
     2281 aagaacaaaa gctatcgcag tactcaatta cttaaaaaaa aggactccac tatataacaa
     2341 aagctataat ttttattgtt tttttcctaa tatatataca ggaagagaga atacaataca
     2401 aattccatta gttacagctg ggtcaagaac cgcattgcaa ctttcggata tttttaaccc
     2461 tatgatagat atttataaag aaaaaaaata tatatttttt acgtctgttt atgattttga
     2521 gggcggagaa cctgtcggag aatatgaatt agtatgcaaa atagcagact tagtaggaaa
     2581 agataatcta attgttaaga cgcatcctag agatgtaaga acaatttata aagataatgg
     2641 atttatcgag gataaaaatt catcaatacc ttgggaggcg attcaattgt caggcgattt
     2701 taacgataaa atatttatga caattaattc aggaagtgtt atttcaggta gtacaatgtc
     2761 tgcaaaacct gtacgcacgt attatatgta taaattgtgt aacatagaag gaaatccgtc
     2821 ttgcaaaaag aatgcgtatg atattgagcg attgcttaag caaaaaacga tgaaaaacat
     2881 tttaaagaat gtgaaaatag cagaaagctt agatgatata ctatgagcaa aactaaatat
     2941 ttaattaaaa atatgggaat attaacaata agtaattttt cttcaaaaat tttagttttt
     3001 ttgttagtcc ctttatatac aagtgtatta tccacaacag aatatggcac atatgatttg
     3061 acaatttcaa cagccacatt attgtttcca attttaacct taaacattgt agatgctgtg
     3121 atgcgctttt taatggataa agataaaaat aaaaaaaata tagcatctat agcgattaaa
     3181 gttatatttt taagcgtgat agtctttgga tccgtaatgt atttgttaaa tattacaaaa
     3241 ttatggcaag atgtttatgg attagaaaaa tatatttttt tgtattatat atcctatgta
     3301 tggaatcaat ttctaataca atttgctaaa gggttagaaa aagttaaagc tatgggcata
     3361 gcaggtatta tcagcacaat tgtaacaata attgccaata ttttcttctt gcttattctg
     3421 aaatggggat taattggttt tttcattgca aatattttat cacagattat ttcagctact
     3481 tatctgtcag tattagtgag aatatgggaa tatatcgatt ttgaggctaa agatcctcag
     3541 ctaaagagag aaatgctaaa ttactcgatt ccattaattg caactgttgt tggatggtgg
     3601 attaatagta cagctgatag gtatgtggtt tcctttatag taggtgtctc ggcgaatggc
     3661 ttattgtctg tggcatataa aatacctcaa ataattaata ctttgcaggg aatatttata
     3721 caggcctggc aaatttctgc aattaaagaa tatggagata atgagactgc aaaattttat
     3781 ggaaatacat ttgttatgat taatttgtta atgtgcataa cctgttcaag tcttatcata
     3841 ttaactagac cgttttcaca tattctttat gcgaaagact tttatgaagc gtggaaatat
     3901 gttccatttt tattaatagc taatgtttta aattgtgcat caggtctgtt aggacctatc
     3961 ttatcagcaa aaaaagattc taaatccatg atgtggtctg caatcatagg ggcattagtt
     4021 aatgtgatta tgagtgtagt ccttgtttat aagatagggg ttcaaggtgc cacaatagcc
     4081 actgttatat gttcatatgt aatatatgtt gttcgtaaac gagctgtggg aaaagatatt
     4141 tttataaagc attatcatat tgttgtggaa tcttggggat tattgtttgt tcaggcaatc
     4201 atagaaatat attttaaaaa ttatgtaata gaaattataa ttatgattat tttaattatt
     4261 ataaatatca gttatattaa gcagttgtgt tatagaggaa gaaacattat ttcaatgatt
     4321 aagagtcgag agcacaaacg gtagacgatg ttatttttat aaaaagatat aatttattaa
     4381 tggtcgataa ttatgaaagg aaaaatattc ttaaaaaaaa gagtatagga tgtcgtatga
     4441 aaatagtcgc tatagtacca atgaagttaa ataacaaaag attgccacaa aaaaacacga
     4501 aagcttttac caatggaaaa ccattgtgtt attatatact ttcaacactt ttaaaagtaa
     4561 aacagattga taaagtgtat gtctattgta gtaacccaga tattaaagaa tttataccag
     4621 aaggtgtaac atttttaaag agatctagtt ccttagatca ggatacaacg agtattacac
     4681 aggtattagc cggtttcaca aaagaagtag aggctgatgt atatgtaatg acacacacaa
     4741 cagcaccatt tattagcgaa aaatctattg aaaaaggact agatgccgtg ctgacaggag
     4801 aatatgattc tgcatttgct gcaaaaaaat tgcaagattt cttatggaaa gacggtgaac
     4861 catttaatta tgaattggac aatattccta gaacacagga tttgccgcct ttatacgaag
     4921 aaactagtgg attttatatt tataatcgca atgtgatgac cgagttgcat agaagaattg
     4981 ggaaaaaacc ttgcatagtt gaagtaggtg agattgagtc agttgatatt gatgaagccg
     5041 aggattttat gattgctgat gcgatttata atcatcttat tttacaaggt agttaagaca
     5101 gggggaaggt taggatgaac aatgtacaga ttttagattg cacacttaga gatgggggct
     5161 attgtaacaa ttgggaattt ggatttgata atacaaaaaa aattatttct ggacttatag
     5221 aatcaggaat tgagattata gaatgtgggt ttctcacaaa taaaacagta tacaataaaa
     5281 atgttacaaa atatacgacc ttagaagaat tagcacagat aattcctgaa aaaagagaag
     5341 gaaaattatt tgtagcaatg atgaactatg gcgaatataa tatagaggat cttccagatt
     5401 atgatggttc atctattgat ggaattagga ttgcgtttca taaaaaaaat tgcaatgaag
     5461 cgttgaaatt atgtcgtgag ataaagaaaa aaggctactt agtttttgtt caagctatgg
     5521 tatctctcaa ttatacagat gaagagtttt tagcagtaat aaaaaatgtg aatcagttta
     5581 aaccatacgc tttttatatt gttgatagtt ttggaatgat gaaaggaaaa gatttaactc
     5641 gtctttttta catgattgag cataatctag aaaaagatat acagataggt tttcactcac
     5701 ataataacat gcagcttgca tattctaatg cacaaaaact tataaccgta caaacaaatc
     5761 gaagcctgat tattgattct tcggtgtacg gaatgggaag aggagcaggt aatcttaaca
     5821 cagaactatt tgtagaatat ttgaatgata atgcaggaaa aaaatatcaa ttaaaaccat
     5881 tattatatat aattgatgaa atactaaata gtttctatca gaagagttat tggggatatt
     5941 cattaccaaa ctatatttca gcagttcata atgctcatcc taattatgca agctatctgt
     6001 catataagaa tactttaaca attgaagcaa tgaatgaaat attcgaaatg atggatgaag
     6061 aaaaaaaggt atcttatgat aaacaatata tagaagattt atactacaaa tatatggaaa
     6121 ctgggaaaat acaggaggaa tatatggaag aactgagaaa atttctctca gacaaaaaaa
     6181 ttctactgat tgcaccagga aaaagttctg tagatgaggt agaaagaata aaagagtttg
     6241 ttaccaaaga agaagtcgta ataattagtg taaattttga atattctcat atagatatag
     6301 attacgtatt tttaagtaat ttaagacgtt ttcatgaatt accgaaagaa agcagaacta
     6361 aatgtattgt tacatcaaat atacaagcat cggatgtata tttacggacg agttataaaa
     6421 agttgttatc tgaaagggaa gttgtaagag acaatgcagg attaatggca attagatttt
     6481 tagccgatat gaatgtggat gaaatattct tagctggatt tgatggatat tcacataatg
     6541 aagatgagaa ttatggtgag caatccatgg aaattataac aaaatttgca ttattagatg
     6601 caatgaacgt gcaaatgaga gatgaattgt cagaattagc taaaaaagta aaggtgacat
     6661 ttttaacgga tccacgttgg gttcgaattg ataagtagta gtaataatgt ttttaaaatt
     6721 ttgataaaga gatagtgaga gaataaaaat gggtaaagtc ttaaataaaa aagaagaaaa
     6781 atatttaagg gacgatgggg cgagatgaaa ctctaatttt gaacttttaa gaatcatttc
     6841 tatattgtgt attgttgctt accatgctaa tttgcgttta aattataatg cagacggaat
     6901 tatagtacat ggttcattaa ctgctaacca gattttcttg aattatattg gtatttgggg
     6961 tcagctagga gttaattgtt ttatatttat ttcggcatat tttttaagtg agaaaagaaa
     7021 cattcacttt gaaaatgttt tgggaattgt attagaatgt attttttata gtgtattagt
     7081 ttatggaatt ctttgtgcat gtggaataga aacattttca ttgaacagct ttgtaaaaga
     7141 aatattatca tttgctctag gtagatattg gtttattaca tcctatttag cattgtatat
     7201 tctaagtccg tatttaaata tcatgcttaa caatctggaa aaacttcagt tgcaaaaaat
     7261 aagtatgtta ttgtttatag gtgtatgctg ttatcgtatg ttatttaaaa catctttgtt
     7321 tggggattgt gctgttttaa ttgcactata tgtcatatgt tatacactga aaaaaacgat
     7381 aataatattt ttgagagaca cagaatcata ggaacaattg tatgtataac agttatacca
     7441 cttctcatat ccataggaat acttttcaaa tttataccaa atgcaatggt taaccagatg
     7501 gttcatattg gttctccatt gatggttata gcaagtatat ttttatttta tgtatttaaa
     7561 aatttttcat tttacagtaa gtatataaat aaacttgcga gaaccagttt aggggtatat
     7621 attttacatg aaaatcatta tttcattgat ggtatgaagt ctagaggctt aatatgggat
     7681 gggatatttg gattaggcaa aaggttttta gatagggatt tttggatttg gtttataatt
     7741 gcagttgtag ttgtgtgcat aatttgcatg gctatcgatg ttgtaagaga aaaatttgaa
     7801 agacagattt ttaaaattaa gagtgtaaag aacctgtgta aacttatgaa tcatgttata
     7861 aatatatacc cataattaat aaatgaggag aaaagaagtg aaagttatat cattcggaga
     7921 attattattg agacttgcag cacccggtta ttcaaagtta tttcaaagag atagtttgga
     7981 gactacattc tgcggagggg aagctaatgt agcggtatcc ttagcaatat ttggatatca
     8041 atcagaattt ataacaaagc ttccggattc agatgttgga tatgcggcag ctaatgcttt
     8101 gcgttatttt ggagtaaatg ttgataatat tatatttgga gagggacgca tgggactgta
     8161 ttatttagaa aaaggagcag ctcagagacc atctaaagta atttatgaca gaaaattttc
     8221 tgcaatttcg ttagctgata gtgatgaatt taagtggaag gagatcctaa acgatgcaaa
     8281 ttggtttcat tggacaggga taaatccagc attgtctgag aatctctttg agacgtcctt
     8341 agatgcatgt caggctgcca aaaaaagagg tataacaatt tcttgtgatt taaattatcg
     8401 atccaagctt tggacatcag aggaagcaaa agaaaagatg gaacagatac taccattggt
     8461 tgatatttgt atttgtaatg aagaagatgc agaaaaagtg ctaggaatta aagctgagca
     8521 tacaaatatt aatagtggag aattaaataa agatggctat gtgcaggtgg ctagggagat
     8581 tcacaagaag tttggatgta ggtatgtagc tacaactctg cgtagaagtt attcggcaag
     8641 tagaaatggt tggagtgcaa tgttatacga agcagaaaaa gataaagcgt atttttccac
     8701 agaatacaac atacaaattg ttgatcgcgt tggtggtgga gacagctttt ctgctggtat
     8761 aatatatgcg ttatgtgaaa atatgtccgg acaaaaggct attgaatttg cgactgcatc
     8821 tagttgttta aaacatacta tagaaggtga ttttaatcga actacaattg aggatgtata
     8881 taatttaatg aatagcggtg gaaatggtcg cgtgttgcgt tagtcaacat ataaagataa
     8941 atcacagtaa tttttatggt gattatctgt cttagtagtg attagaataa cgtgaaaata
     9001 aattgttgag cgtttatact gttttgaaaa ataaatttaa catattgaaa aatacaaata
     9061 tatacgtata ttataaagcg ccgattgtaa aattaagcta gacaactaaa acagtcattt
     9121 gtgctacact caaaatagtt ccgaccaaag aattataagg agtgagtgca aataacctat
     9181 acctatctta caacggatga acttgtaata atagagtctt atttgaaaat taaccaatct
     9241 gttgctaaaa ctgcgcattg cttgaatcgt tcaagtcaaa cgatccataa agtatatctg
     9301 ttcttcaagc aaagaaaatc agccttagaa ta
//
LOCUS       DAYYFU010000087         9280 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000087 DAYYFU010000000
VERSION     DAYYFU010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.092"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACHA34_002517"
     CDS             <1..435
                     /locus_tag="ACHA34_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8793108.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACHA34_002518"
     tRNA            complement(759..842)
                     /locus_tag="ACHA34_002518"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACHA34_002519"
     CDS             1370..1870
                     /locus_tag="ACHA34_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8793109.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="ACHA34_002520"
     CDS             complement(2060..2404)
                     /locus_tag="ACHA34_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFM8793110.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="ACHA34_002521"
     CDS             complement(2558..3478)
                     /locus_tag="ACHA34_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8793111.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="ACHA34_002522"
     CDS             3665..4156
                     /locus_tag="ACHA34_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFM8793112.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="ACHA34_002523"
     CDS             complement(4180..6555)
                     /locus_tag="ACHA34_002523"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFM8793113.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="ACHA34_002524"
     CDS             6881..7507
                     /locus_tag="ACHA34_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8793114.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="ACHA34_002525"
     CDS             7530..8234
                     /locus_tag="ACHA34_002525"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8793115.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="ACHA34_002526"
     CDS             8426..9259
                     /locus_tag="ACHA34_002526"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFM8793116.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2828 a         1791 c         1651 g         3010 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct
//
LOCUS       DAYYFU010000088         9001 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000088 DAYYFU010000000
VERSION     DAYYFU010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.093"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            213..1427
                     /locus_tag="ACHA34_002527"
                     /pseudo
     CDS             213..1427
                     /locus_tag="ACHA34_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1606..1935)
                     /locus_tag="ACHA34_002528"
     CDS             complement(1606..1935)
                     /locus_tag="ACHA34_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8793117.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(1925..2188)
                     /locus_tag="ACHA34_002529"
     CDS             complement(1925..2188)
                     /locus_tag="ACHA34_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM8793118.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2648..3451)
                     /locus_tag="ACHA34_002530"
     CDS             complement(2648..3451)
                     /locus_tag="ACHA34_002530"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8793119.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3429..4553)
                     /locus_tag="ACHA34_002531"
     CDS             complement(3429..4553)
                     /locus_tag="ACHA34_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8793120.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4569..5384)
                     /locus_tag="ACHA34_002532"
     CDS             complement(4569..5384)
                     /locus_tag="ACHA34_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8793121.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5374..6246)
                     /locus_tag="ACHA34_002533"
     CDS             complement(5374..6246)
                     /locus_tag="ACHA34_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8793122.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6331..7620)
                     /locus_tag="ACHA34_002534"
     CDS             complement(6331..7620)
                     /locus_tag="ACHA34_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8793123.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7764..8276)
                     /locus_tag="ACHA34_002535"
     CDS             complement(7764..8276)
                     /locus_tag="ACHA34_002535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793124.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8343..8486)
                     /locus_tag="ACHA34_002536"
     CDS             complement(8343..8486)
                     /locus_tag="ACHA34_002536"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8793125.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
BASE COUNT         2793 a         1906 c         1439 g         2863 t
ORIGIN      
        1 atgattatcc attgagagac tcctttggta ggatgacgtt ttggtcgact aaattttacc
       61 aaaagtgagt ctctttttga gttgaaacga agaaaaagtg ttagtagaaa atcgccttcg
      121 attttccacc aacacaaaat attatagaag caaaatcaat tctaccttag atgttcttat
      181 atcactctat cagtcctatt tgacaaagag ccaagaaaaa gacgttggtc gtcgatgctg
      241 ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta gatggaaacg
      301 ggaaagcaat tatagtgaaa aatgggaaga aggaaacgat tgtccgtttt gaacaataca
      361 atcatatgcc tttggttacg cgcctaaaaa agcaacgcta tacctgtaaa aactgtcgta
      421 cccattggac ggctcaaagt tattttgtcc gactcagcca ttcaatcgca gatcatgttc
      481 gatataaaat tgcttcttta ctgactgaaa aagtatcttt gtcttttatt gcgaaaagtt
      541 gtcaggtatc tttgactacc gttactcgta cattgaaaga gtttaaaagc tatttaccaa
      601 agccttctaa gaagattctc cccagagtat tgatggttga tgaatttcgt tcgcacgctt
      661 ccatagaaga taaaatgagc tttatttgcg cagatggtga aacaggaaac ttaattgctg
      721 tcttgcctac gcgtaaatta cctcgattaa caagctattt cttaggctgc accaatccag
      781 aagaagtaga attcttagtc acagacatga acgccgctta cttccagctt accaaacgtg
      841 ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tgtcaaacac atgaatcagg
      901 cactcaatga gttgcgtatc cgtgaaatga atgagcttcg taaagcagga cagaaaagtc
      961 aggcagaaaa actgaaaaag aactggcgct ttctgctaaa aaatcgtgca agcatcaacc
     1021 attatgagta caaaacatgg aaaagtttcc gagcaccaaa atacccattt ctcactgaag
     1081 caatgatgat tgatcgattg cttgagtttt ctgcgcctct aaaggaggcg tatccctttt
     1141 ttcatgaatt agtcgaagcc tttcgagaca aagaccctga cttatttttc tccttattgg
     1201 cagaacttcc cgaaacgtta gatgacggct ttcgggagaa gcttcaaaac cttctgacct
     1261 atgaagaagg tatcaccagc gcaatgatct atccttattc caatggaaaa attgaagcga
     1321 agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca ttcgagaaca
     1381 tgagaattag aatctttttg atcaatcaat taatcaatgt aagataacaa aaaatctgaa
     1441 ccagaatgtg gttcattctg acccggattt gattttgcac aactcatcag tccttattga
     1501 caaagagcca aaaatctgag ccagaatgag gttcattctg acccagattt gattttgtac
     1561 aacccatcag tccttattga caaagagccc ttctttttag tattattata attcttccat
     1621 gcgttcttcc agtcgctgta aatcgcttgg acttaattcc ccaatttttt tccatttgac
     1681 ctttttgttg tttttgggta attcgtttag agttcctacg tcaatccaag attctttttt
     1741 tagtcctgcc tgtttccaat cccttatttc aacgtatttc agctttattt tatcggattt
     1801 agtatgatat tttgaagtaa ttgacagaaa cgaaattgtg tccagtccat tttttagaat
     1861 atagacaggc cggcgtttac cgccctctgc agctgtgaaa gcaacgtaga cggttgctat
     1921 atcattagta ttcatcttca ttgaaccatt cttcaatttt ttcgtcagtg tctagttttt
     1981 ggacaggcaa tttttccgct aatctttgaa tattatggat tcgtttactt ttttccgaca
     2041 attcaataga aaatgggatt tttccttgct caacaatttg cttataaaaa acggtgacgg
     2101 ctgttttggg gctcatgcct agttcatcaa taatgctttc tgcttcattt ttcaattttt
     2161 cgtccataga aatttgtaac ttagccattt tctccacctc tttctgacaa acacattata
     2221 tcacggaaag agttcaaatg gaattaatac aactactttt gaactccttg aaatgcagtg
     2281 tatttatcta aatataagga aaggatatag tgctaaatgg ccttgataga tagaaaagtt
     2341 tcaaaatccg ccacagcgat aattgaagcg aacactcaac gacacacaga acaggaaaag
     2401 actcctaaaa gaaccaaatt aactaggacg tgtctgaaaa ctccacaaag atgtggtatg
     2461 ctctggtaaa aaacgaatgg cacaaccacg aagaccgata tggttcttgg aaaactttct
     2521 ccaccctttt tgtaattgga gggatagtgg tttattgata gcgattttcc aagttcttca
     2581 agtggaacca gacattgagc accacggcta aaaagtgaat gaagtcttta tttttaatca
     2641 ataattttta atgattacca gtaattggtt ttttcatgcg ccctatttct atttgatgtg
     2701 gagttaaata atgctgataa gtaaaaattt ttccgtcaat taaactgcca ctagtgaatc
     2761 ctgttctatt ggtaaaaaga gaatagtctg ggtattcatc tacaccaaat gataaagatt
     2821 cagccataaa ctgtttttta ccagcaaatt cagcactgct accttgatgg atatgtccta
     2881 caaaaatacc taaaatatca ctttcggcaa tcaattcagc aaaaccttcc ggaacttcag
     2941 ttacaataga tggatcttcc cacgacaatg gatgatgaac caataaaatc gttccctttt
     3001 cagcaggtgt acccaaccac tcttgtaatt gaatcatctg tcctatcgat atttttcctt
     3061 cgtgctgggt atcttgagca gaatccaagg aaataattcg taacccattt tgattcgtac
     3121 aatggatata ctctcccgtt tcattgcttt caacactcat tccttcaaag aaaacactgc
     3181 gacgatcatg attcccacga caaaaatagt aaggtgtggt tgggaaatat tgatccaaca
     3241 ttttccgtaa gacttgataa tcagaaatcc ccccttcatg tactaaatca cctgttaata
     3301 gaataaaatc gtacactgtt aacgagagtt cttgtaacaa tttttccaat gccaactgtg
     3361 gatttttaat tgttggaaat agttcagcag taaacaagtt agtttgatag ttttctctaa
     3421 aatggatatc agagagatgc aaaaagcgca tggctattcc ctctttcttc aataaaataa
     3481 tcaggatatc ctaaattttc ttgcgtctga taatcaaaaa aatgcattct ttcaggttct
     3541 atgcggagat tgactgcatc accaatctgc caagtaggtt tttcattaag gacaatcatt
     3601 tctataccat cgactttaac ggtgtacgca taactttgac ctaatagctc acgatacttc
     3661 acttgaccgt gtaaatcgct tgtttctttt gaaaaggtta cgtgttctgg tcttatgcct
     3721 aaatacaatg agccactaac ctgaagaagt tgtttttgcc aagctggatt caatgttaaa
     3781 aattgactgg ctacctttaa tgttccttga tcataagtaa ctggcataat attcatgcct
     3841 ggcgaaccaa taaaatttgc agtaaaagta tttttaggtt tcgtataaac atttttcggc
     3901 gtatcaatca tctgaattct accttcattc aataaaacaa tccggtcagc taacgtcatc
     3961 gcttcaattt gatcatgagt gacgtagaca aaagtttgtt tataacgttg atgaattttt
     4021 accaattctt ttcgtgcatc caatcgtaaa cgcacatcca aatttgataa cggttcatct
     4081 aataaaaaat aattactccg tttaaccacc gcacgagcca atgctacacg ctgccgttgc
     4141 cctcctgaca aatctttagg aagtcgatct tggtatggcg ttaaacctaa catcttcaaa
     4201 gcttcttcca aacggtgctg aatgtcagct gtcttaattt tattcgccct taatgcaaaa
     4261 ataatattct cctttacagt catgtgagga tataaagcat aattttgaaa aaccattgcg
     4321 atctctcgtt ctccacaagg tagcttattt gctagttgtg tacctaaaaa aagctctcca
     4381 ctcgtaattt tttccaaacc agcaatcatt ctcagcaaag tagattttcc tgaaccagat
     4441 ggtcccagta acacaattcg ttctccagcg gccacagcta aatcaatttc ttttaaaatg
     4501 gtatgttctc catatttttt tccgatattt cttaattcaa gcgtttctcc catgatttgt
     4561 cctccaattt atttaatacc tgcagaaaca aaagtcccga taatatattt ttgaaaaatt
     4621 gcatacaaga ctaacggtac aaacattgta attaaactaa tcgccattgc tacggtaaaa
     4681 tttgtcccac cttcagaact aatataggtc tgcatcgcca acggcaaagt ataattatct
     4741 actgtccgta aaattaatac cggccaaaca tactcattcc aagaggtaat aaaaaaccaa
     4801 ataccggtag aagtaatcgc atgtctagta agaggaaaaa caattttact catgacggtt
     4861 gtttgtttga tgttatccaa tgccgccaca tctagaaggc tcactggaat ttcccgcatt
     4921 gtttgcgtca ataaaaaaat gcccatcgca tcagcaaatt gtggtattat cacaccccaa
     4981 gtagtatcaa ttaaattaag ttttgcaatc aacaaataat taggaatcat cgcaacagta
     5041 aaaggaatga acatcgtacc aatgatgata aagtaccaga ttttcttccc tttgattcgg
     5101 caataagtaa aactatatgc cgcaaaaaaa gctacgccta acttaacaac cgttataatt
     5161 gtagcaataa aaaaagtatt tctcgtaatt ttgataattg gaagattttc gaacaaatag
     5221 caaaaatttt ccaaggtaaa cggctgtggt atcaaactag taatcgtctg ataggcatct
     5281 ttcaatggct taaaagaatt ggccataata aacaataatg gatagataat aatgattatg
     5341 atgaacataa ataagagatg tggcaaccag cgtttaattt tcatagtaaa cccccttttc
     5401 cacaaattta aatattaccc ctaagaaaac agcaaatatt aataaagtta aaactgaaat
     5461 tgctgaagat tctccgactt tatacatttc aaaagccttt tgataagcat aatagatcac
     5521 gtttgcacta ccattattcg gtccaccacg agtgatgatc ttaatcggag taaacacata
     5581 ctgcagacct tgaacaatgg tcatcaccaa taaatatagt cctgtcgaac tactcatagg
     5641 caacacaatt gtaaaaaaaa tgcgccagat gggtacacga tccaacttag ctgcttcgat
     5701 catctcttga tcaattccca caattgctgc aaaaatgaca atgaaattat aaccaaaaac
     5761 tttccaagca gtaatacctg caatcacagc aattacccaa ccttgtaaat tcatccacat
     5821 aggcagtctt aaaccaaaat ttcccaacaa gacagcaatt ggtcccgata ccggatttaa
     5881 tatccatgta aaaaccatcc ccgccactac caaagaaatc acagctggaa taaaaaataa
     5941 tggtttatac aaattctgcc ctttttctat aactattcct aataaaaagg atgtaatata
     6001 tgggaacaca cagctcagca agacaaatag aacggtatag gacaaagtat tcttcaacac
     6061 tttatgaaag actggatcag tcagcacctt tacatagttg gcaaagccaa caaattcttt
     6121 agtgggggcc accatattcc agtcaaaaaa actcaaatag atcgtataaa tcaatggata
     6181 aaaaacaaac acgcccaaca aaataattgc tggcgccaga tacaaataac ctacatagcg
     6241 cttcatttcc gacagcctcc tcatccttat gtcaaaagca gacgaccttg tttttccttt
     6301 cttacaggcg ctgtcgtctg cttgctttgc ttatttgagt aactcattaa tttccttttg
     6361 agtagctttc atcgtttcat tgacggaatt acctgataaa attttatccc gcatatccaa
     6421 caatttttgt tcagcttcta atccagcatc tccaggaaat gaactccaac tgaccatctt
     6481 atccatctga tcaattgctg gtatcatcat ctgattttca gctaagaaag cttttaatcc
     6541 attttgggcg tctgcaacat ctttacgtgg tggtaaataa ccagtacctt ctgtccattt
     6601 agctaaagat tcaacactat aaagatattt taagaaatcc caagttgctt cctttttctt
     6661 atccgtttgt gatgtaacag ctaacattgc gccaccagca ggtagtttaa cttttttccc
     6721 ttcccaactt ggtgactcaa ctgctttagc agtaaaagta ctattttttt gaatgttgtc
     6781 cctttgagca atagtggtat agaccatcgc gacgttacca tcaataaagc tttgcacacc
     6841 ttgttcccac gtagtgtgta atgccgtctg gtcttttacc atcctttgat ataattcata
     6901 agcagcgata ccatctttag aagcaaaggt agctttgcca tcttttatta cacttgcccc
     6961 attactttct aaaattgctt gttgtgccca attatcagca ggttcttgaa tatacacgcc
     7021 atactttcca gttttttctt ttattatctt agaaaaagtt tgaacttctt cccaagtttt
     7081 tggaccattt tcatccaagc ccgctgcttt taatatatct tgatttaaat ataaaaccgg
     7141 attactaatg gagtaaggaa tacccacttg tttaccatct ttttcagaca gtgccatgat
     7201 attgtctaaa aaatgatctt taaaataatc agcattatcc ggatccactt cttctactaa
     7261 agattgagga ttagtatatt tgaaattttc tgcaaaataa tctttaaaag cccatcccac
     7321 ttgtactacg tccggtattt ctttggaggc aacagaagct tgtaaatttt gcatcaagcc
     7381 tttatacata tcaggattat attttgctac aaccttcact ttgtcttgag attgattgta
     7441 tttatctact aattcattca ctgtttttcc acctagggta tccgcattaa catgccaata
     7501 ctctatcgtt acacgaccct ccgatttttt tgttgtcttg gtattcccac taccacaagc
     7561 tgccaaagag atgacactta aaacagacac aacccctata acaagatttt tctttaacat
     7621 aacattaact tcctcctaat ttctttatgg ccatttttct aatcaagaaa tattatgaac
     7681 taccaatgta aatttctagt taattttctg taaataattc gaaatttgtg catttcgggg
     7741 tacagggtca ctggacatat ccatcaagct aagaataaag cccccatatg gtcatgtatc
     7801 ccatctccaa atctcttaac tcctctgttt tgattataaa atagtttatt ctgtgctcct
     7861 ttcttccata aataggccca tgcacaacgt gaggttatat tataaacacc acagcgcgca
     7921 aggtactcaa tcgtttctag ttcattgact gtccatacag aaaatgtcaa tcctagctgc
     7981 tgccccactt cacgggccaa cttagttaac catccttcat gaatattgac gatttgaacg
     8041 ctttgatagg ctaatttctg taaggtatcg atcaaatctg cttcggtaat atccccaagt
     8101 tttttccctt cgaactgatt ttcaatatta taaaatatat gctttcccca aatcgcagcc
     8161 caagataatt gtacatagcc actaagaaca atttgtggcc agatttgata ggtttgtgcc
     8221 agcgaaaaaa cggccatttc caatccctct tcttttaaat cacagttaat ggtcatggtt
     8281 gccttctcat tggccactgc ttgaaacacc tgctcaaggg ttaccgaatc tgggacaact
     8341 tcttacctgt catgggctag tattagcgta ccatctgttt gctgacgcac atctacttca
     8401 aaacaaagct ggcgatccaa acaaaagcga agaaatgccc agctattctc ctccgtattg
     8461 tcactaccag agtgtgcagt gatcatggtc attttgaccc ctcctttaat tccgtgattt
     8521 tctcccacac gtccactata ccgatgaagc gttaatttct tgttaatttc ttgtaaattc
     8581 cctgcgtttt ttcagtaaac tagttgtaaa tcttgcatgc tccttcctac gaactcatga
     8641 atcgtcgatt ttgcccttct agctagtttc accaattaag tagttctaca ttccccgctt
     8701 ttaacctact acaccttttt ttgttttcag ctattccttc ttcttgctga aagtccttga
     8761 ggttctttct tacaggatac catcaaggtg ttcaatggct tattcccatt gaatgaccga
     8821 gacttactat cagatttctg ctcgttgaac caagtctttg atacaggatt tcttccgtca
     8881 tcttctcaca aataccacct tgcgtttctc tagcgattgg tcgatgtgca ccctcccagt
     8941 ggactgtcac tacccctttt tgctatgcct agcccacaaa aaaagctgtc ctcacagaca
     9001 g
//
LOCUS       DAYYFU010000089         8494 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000089 DAYYFU010000000
VERSION     DAYYFU010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.094"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACHA34_002537"
     CDS             complement(<1..149)
                     /locus_tag="ACHA34_002537"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8793126.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACHA34_002538"
     CDS             complement(593..1714)
                     /locus_tag="ACHA34_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFM8793127.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACHA34_002539"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACHA34_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFM8793128.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACHA34_002540"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACHA34_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFM8793129.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACHA34_002541"
     CDS             complement(4304..5215)
                     /locus_tag="ACHA34_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8793130.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACHA34_002542"
     CDS             complement(5232..6239)
                     /locus_tag="ACHA34_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM8793131.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACHA34_002543"
     CDS             complement(6236..8365)
                     /locus_tag="ACHA34_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8793132.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACHA34_002544"
     CDS             complement(8370..8480)
                     /locus_tag="ACHA34_002544"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793133.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DAYYFU010000090         8314 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000090 DAYYFU010000000
VERSION     DAYYFU010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.095"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(522..878)
                     /locus_tag="ACHA34_002545"
     CDS             complement(522..878)
                     /locus_tag="ACHA34_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8793134.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(892..1302)
                     /locus_tag="ACHA34_002546"
     CDS             complement(892..1302)
                     /locus_tag="ACHA34_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8793135.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1649..3835)
                     /locus_tag="ACHA34_002547"
     CDS             complement(1649..3835)
                     /locus_tag="ACHA34_002547"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFM8793136.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            4053..4736
                     /locus_tag="ACHA34_002548"
     CDS             4053..4736
                     /locus_tag="ACHA34_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFM8793137.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4817..5536)
                     /locus_tag="ACHA34_002549"
     CDS             complement(4817..5536)
                     /locus_tag="ACHA34_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8793138.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(5848..6054)
                     /locus_tag="ACHA34_002550"
     CDS             complement(5848..6054)
                     /locus_tag="ACHA34_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793139.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6299..6901
                     /locus_tag="ACHA34_002551"
     CDS             6299..6901
                     /locus_tag="ACHA34_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8793140.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            7042..>8314
                     /locus_tag="ACHA34_002552"
     CDS             7042..>8314
                     /locus_tag="ACHA34_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8793141.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQ"
BASE COUNT         2343 a         1558 c         1360 g         3053 t
ORIGIN      
        1 gagtgtttcc cgattcgttt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacaact cagttatctt atcatgtcat
      241 ttctaacatg tcaacaacta ttttatatgt tttttaaaat actattttat tgctttgcta
      301 cttaaaacaa cagaaatgaa tatatcatgt aaatttacac atgtcaatat atttttaact
      361 ctatatcccc acctagattt gaattttgac cacataaatt gagcatgtct ctatagtaga
      421 aaaataaagt caaaaaaaga acacataaca cgcttgatgc taagtgttcg actgttacaa
      481 ctgtatgaaa tgctcagtga cttgattaaa acagttaaag tctatttatt taagataagc
      541 tgttccatca actgctcatt gtatccaata aatagaactt catcgataat aatcaaagga
      601 cgtctgagga gattggtatg ttgtgtgcat aaaaaaatca gttgtttttt tcctacacag
      661 gaaaggtcaa tcgcaatgtt gtgaaaagcc tcagaccaag tcgcaaatag ctgttctgct
      721 tgcacttctt caaaactgca taattcttct gcttcttctg tacttagatc gcgttcttct
      781 aagtcaacaa attgataagg caattgatgg tgttctaacc attgtttcgc cttcagactt
      841 gttacacagt gcgctgatcc atagactttc acttccacga aggctccctc cctatgaatt
      901 taagatgtta ttgttttgta aatagatcag atttctggca acacatcgaa tttcacgagg
      961 gacaaatgac cggatctcat cttcattaaa tccgattagt attctttttt catcaatcaa
     1021 aataggtcgt ctcaataatg aaggatattc tttgaataag acgatcattt gctttaaagt
     1081 taattcgtta aaatctatgc ccagcttttc gtaaattttc gaccgagtgg aaataatatc
     1141 ttgaactcct ccttcagtcg ctgccaagat ctttttgatc tcactttctg ttaacggttc
     1201 ggcaaaaata ttcttctctt caaattcaag ttgattcgct tgcaaccatg ctcttgcttt
     1261 tctacaggaa gtacagctag gcgaagtata tacttttatc attttctcac tcatttttaa
     1321 ttataatcca attctacagt aatttctttt aaaataccaa ttattagcta ataatatata
     1381 aaaaaaaatc aaaataactg attatttacg tttgaataat gagaattttt ctatatattc
     1441 tctattttga taatagtatt ctcattttca aaataaataa gggaggtgtg acaaaattct
     1501 tgtcacacct cccttttccg aataaactgt gttccagaaa tagcttcttc gaaaataagc
     1561 tgaaatttca caaaaatttg agaagcaatt ttcgtacatt ccttcttctt tcttgaaact
     1621 gaccacttct gtcacaccct ctcttctatc actttccttt tttcacacga ctcccggttt
     1681 ctttgataaa tctcgatact ttcaattttc ttgaattttt catttgaggt acacatagat
     1741 acagacgatt tcttgctcta gtcatcgcaa cataaaatag tcttcgttct tcttctatcg
     1801 tcgtcactgg ttctgctttt atttcattaa agtcgattac tttcttttct gtcgctccgt
     1861 tataaggcaa acttccttcg ttacaaccaa tcaaaaagac atttcggtaa ctaagacctt
     1921 tacttttgtg aatgctctgg atacatagtg catcaagttt tccttgtctg taagcaatca
     1981 agcgggtttt taattcttta gctgtcgctt gggtttcttg cgataaggct tggagtcttc
     2041 tgaaagtagc atgctgcttt acttcttctc gtatccattc ttgatcactc tggtcgattt
     2101 ccttagctat acgtggcaga tgaagatagc tttgaacaag cgaatagatt tcttgatgct
     2161 ggtctgtttt acgcaacgct tcctcaaact ctcggatttc tgcagatttc gattttaaat
     2221 tggggcttat cgacaaaata taatctgcaa cggctatatt ttgatattta ggagaaaagt
     2281 attgatcgca ttcacttttt ttcaggtagc agtctgccat cagctgccgg agcaattgct
     2341 ggtaggcctg atttttcttt tgcagattca gtgctgtttg ccaatgaaca attttttgat
     2401 agaaatgttg gaaagacttg ataccaaaga taccttcata tggactatct gcttccagta
     2461 caaaaggaat attcgttttc agcagagatt ggacaagctg ctgcgtttgt gaagctgatc
     2521 ggtataatat gattgattcc ttatatttcc catttttttg aataaatgtc tgaatttgtt
     2581 tgattagcca ctgactttct gctttagtat cagaaaacaa ataggcttcc ataacacctg
     2641 cttctttttg tgctggaatc atcgcctttg gtatccgatc atttttatta tagctgatca
     2701 atctatttgc tccttggaca atagctgccc gagaacgata attagtcaat aagaaaagag
     2761 ttttggtagg atattctttt tcaaagttct tgatgtatgc tggctctgat cctctgaatc
     2821 catagatact ctgatcatca tctccaacaa caatcaactt gatcctgttt tcctttgtca
     2881 tccgtttcac gatttcccat tgaagtggat tagtatcttg aaattcatca atgaatagat
     2941 aatcatattt ttggccgata taatgctgga tctcctcatt ctccaaagcg cttttcagat
     3001 tttggagtac atcattgaac tctagcaatt gctggcttct tttttgttct tggagttccg
     3061 tgagcaatcc atacagtcgt tctgatatcg gctgtcgtgc attcttatca atggaatcat
     3121 atttttcttt aactaactga accatttctt ctactgaaag gttcatattc tgccaatagt
     3181 ctaaccgaga agcaatcgtt ttttctccat attgatcaaa tatataaaga ttatatttac
     3241 gtatcagctg ttgcaaaaca gcattcactt tttgtgcagt atcctttgaa aagtctcctt
     3301 gtattccttg cattgctact tcaggaaatt ttcgacaaat ttcattgtat atcctaaaaa
     3361 agatactatg gaatgttccg atatggacag cggtttttct ctggctgaaa ttatctactc
     3421 gtttttttag ttcatttgca gcgttccttg taaaagtgat cattagtatg cgttcttcgt
     3481 ctacttgttt ttgcgtcatc agataaacag cttttgcaca gattgtttgc gttttgccgc
     3541 tcccagctcc tgctatgatt cgaagatgtt tcttcatatc atatgtgata gctgcttttt
     3601 gttcttgatt gaagcgatta taggaacgtt tctccatcat tttaaagaaa gagctgtttc
     3661 cttcactttc ttttacttgt acttcttcct caataagtcc gggtgcttcg ccataagccg
     3721 cttcataggt agcaatgatt tcttcttgta gccgaaagac ttgttgaagc tttttatact
     3781 tgttctccca gcgtttcaat tctttttgta atcggagaat ttctttttca ttcaactgat
     3841 tgatttcctt cccatctttc ttctttgaat ttgtttattt caagttggtt atttaaagtt
     3901 tcgttgtcta ttttttcttt atactaacag acctttctaa aaatgaaaat gggaagcgat
     3961 atgattttag ttttctgaaa atttttcctt tttttcaatc gtttatgcta agataaggaa
     4021 gacaaaaata ttcataaaag gtgatgaaaa aaatgcagac ttcctttcac gaggggcgta
     4081 caataggact tttgctgaca tttatcggag gagccatgga cagttatact tatatccaat
     4141 atggtgcttt tgcctctgct caaactggca atatcattct atcgatcatc caagcatttg
     4201 acggagaatg gctaagtgtt ggtaaaaaag tattgtctac ccttttcttt ttcttaggga
     4261 ttcttctaac aaagtatcta attgattatt ttagaaaaaa agaaaaacat ttctggcgct
     4321 tgtttgttct atattatgaa gcagtgattt ttttctttgt tagtttgaca cccgtccatg
     4381 agcatcccgc attagtaacg attttgattg cttttactgc atcgatccag tgggtagctt
     4441 ttgataaaat caacgggcgt gcctacacta atttatttac aactggaaat ttaaaagggg
     4501 tagcaacgaa cttatatgat tattgtattt ctaaagaaaa agcagatttt gatcgatttt
     4561 tccactatct aagtgtcgtt cttgccttta tcgcaggagc tattgtcctt gttttctgtc
     4621 atcatctgtt tggcgcccat tcgattttga tcgtttccat tttatttttg atcatggccc
     4681 tcactcagac atttcattta tggcaatttt atcatacgaa tcggttcact atttgatctc
     4741 aatgaatcaa aaaaactaag caagatgctg ctcacagaga aatatctgcg agtgagcaat
     4801 ttgcttagtt tttatattat ttatttgtaa agattccctt tgctttcggg ttatttgtag
     4861 tgtagcggat cgactgggca tctgcctctt ctgtcatttt ctttgcccgc atcttgattt
     4921 cttgtacgat ttgttctcca catgccggat cggctacgaa atcatatttg tccccatcga
     4981 tcactatttt gggacatata tcaaactgtt cataccactc ttcgtatcgt cgattgagtt
     5041 ctttatagta ggaatagagt tcaggatctt gttctagctg ttcatattcc cggcctcttt
     5101 ttttgattct cgctaacata gtttcaaagg atacacgaat ataaaccagt agatctggtt
     5161 tcttttgtgg cgaaatttct tctaactcct tcagcatcgt ttctaaaaga ccttcatact
     5221 gctttacttc tacttcagaa acacgcccta aatctgcatt caaatgaaac agcatactat
     5281 cttcgtagat ggagcggtct agtacgttgt catcatggct gaaagcatct ttgattgcca
     5341 aaaagcgttt gttcaaaaag aaaatctgta ataaaaaagt atacttttct ggattagaat
     5401 aaaatagcgg caatacttca ttatcttcca cgttctcata aaaaggtttt gtttctaaat
     5461 cttgggcaag catttcagtt agtgtgcttt tccctgcacc gatcgttcct gcaacaatga
     5521 tcacaattat cggcatcctt cctgtaataa aatctgttct tcattttact acttcattta
     5581 tagaaataaa agccttgact ttatctgctc cttaaaagct acttctctaa aagtagccaa
     5641 aagtaactcc tattcatgaa aataaaggct gtgacaaaag tcttgtcgca gccctttttt
     5701 caaataaaca gtattctcct gccaacacct cggtcacaag gcaatattca taaaacatga
     5761 aaagctgttt tttaacatta ttccttgtaa ctcagagcta aacgggagac tcacaacctc
     5821 tttatgctct caacatttta tctgttttta ttcttctatt gtacttgatt tgcgtacaac
     5881 taactgccag tgcagattat gagcggtatc attgtcttct actgcgcatt tcatgacacg
     5941 tatcatcaca ctattttcat agattttttc tatttttcct tttattggtg attcaaataa
     6001 atcagattta aatgcatatg tttcgccgac ttcaaacaat tgttcatttt ccataagaat
     6061 ccctccatca ccttttataa ggtcagtaat tttttaacat aaatcactcg aaaaagcaca
     6121 aacttgtcat aaaaagtact tttcgtatca aaactgtcac tttttttggt aaggttcttg
     6181 gaaaacgaag atttttttag gagatcatga cggttttgat gtcaaaacca ctttttatga
     6241 aacaaaagac gttttttatt ttttttagca taaaatttgt ggtggaggta cgtatgagat
     6301 gaaagataaa gttgtgaaca aattattaga agccttattc agcggagaca aatcggaaga
     6361 agatatggtg tatgtccaag taaaatttgc cttagaagtt ttattgaata atcttggaaa
     6421 actagcggtc gttctagtct tttctctggt gacaggctca tgggcggaaa caggaatcac
     6481 ctttctttct tatatctgca tccgcagata tgcttatgga ttgcattccg actctgaatt
     6541 cgtttgtctg ctgtggacac tactttattt atggggcgtt cccttggtga tgaaacatct
     6601 gcaattgact atctccttcc caatgatggt ctttttgtta atcagctgct ttttgttact
     6661 gcttcgctat ggatcaagag gtacagcaat caatcccatt gaacccgaga aacgtccccc
     6721 attattaaag aaggcgatca gcatgtttct aattttttct ctcattactt tatttttctc
     6781 agcttcttac ttttctacct atttgctact tgggattgtt ttagaaatag ctacgttgct
     6841 tcctattaca aactacttga tgaattttgg aggaatcttt catgaaaaaa acaatcatta
     6901 aacaattaac taacttatct gacttttttg tatctggctg ctgctttggt atgagttctg
     6961 aattcaaaat ccctgaaaaa ttaaaaaaat aatacgctaa ttgtgctata atttggtaaa
     7021 aatcaatggg agctgtactt tatgttttta tggtccatag cgttgcaata tttgatttac
     7081 acaatttgta tcttattgat tgaccatcac atccgataca aatatctgtt tgcgggagtc
     7141 attctgattt cttgtatttt gattggatgg gtctattcga ttttagggat ttttactgct
     7201 ctaattgcta taggaatgct gatgcttact tttttcttct tttcgacttc gcgtcaacaa
     7261 ttggtttacg cgcttccttt tgggttattg acgtcgcttt taggagatca cttaacaagc
     7321 atcgacgatt tttttgtttt gaaatcttcg gttattgaac caggagattc attgcagtat
     7381 tttcacattg tcttatccgc ttttttatct gctttattag ctttttgttt aaaaaaatcg
     7441 ttagagactt ggtttcacac tgtggaccgc cgtatgatcg gtacgatcgg gacattgata
     7501 ttatttacat actatatcat tattttttat acccgttttt ctggtgaaac acctgatgtc
     7561 ttagctttga atacgagttt tttcattcta tatctgtgtg ttggcctgat catcttggtt
     7621 tattattgga aaatctctaa taagaaatta gctgatcagt tattagagca acaagtcagg
     7681 atgcaacagg attatatcaa agatctagaa aaaaactatc aagacttgag agaattcaaa
     7741 catgattacc aaaatctgct tttttctctt cacagctata tttcagaagg cgacttagaa
     7801 ggattacggt cttattatga tcaaacgata ttacctacac gccagatgat gaaccttttt
     7861 ccggccaatc taagtatgtt ggataatatg aaagttcctg agatccgtag tttactgtca
     7921 ttaaaactca tgatggcaca ggaaaaaggg ttgatcgtcc agcttcattt tcctgagcca
     7981 atcgaactgg atacgaaaca tacggtaaac cttgtacgta tgcttggtat tatcttggac
     8041 aatgcgattg aaggagctag tgctgcaaag aagaaaaaaa tcgaattgac catactcaag
     8101 aagaatcatt ccgtgatgat tcgtgtgatc aatacaacaa caaatacatt accgttaaac
     8161 cagttgaaac aaaaagggtt ctcaacaaaa acaaatccaa aaaacgaagg ccttggcttg
     8221 tttatcttgg atgaattgac aaagaccaat ccttatcttt tccttgaaac atcaattgaa
     8281 aaccagcgtt tttctcaaac gatttatata caaa
//
LOCUS       DAYYFU010000091         7926 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000091 DAYYFU010000000
VERSION     DAYYFU010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.096"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..199)
                     /locus_tag="ACHA34_002553"
     CDS             complement(<1..199)
                     /locus_tag="ACHA34_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793142.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
     gene            complement(362..1996)
                     /locus_tag="ACHA34_002554"
     CDS             complement(362..1996)
                     /locus_tag="ACHA34_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8793143.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            complement(2000..3373)
                     /locus_tag="ACHA34_002555"
     CDS             complement(2000..3373)
                     /locus_tag="ACHA34_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8793144.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            complement(3389..3628)
                     /locus_tag="ACHA34_002556"
     CDS             complement(3389..3628)
                     /locus_tag="ACHA34_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793145.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            complement(3810..6485)
                     /locus_tag="ACHA34_002557"
     CDS             complement(3810..6485)
                     /locus_tag="ACHA34_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFM8793146.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            complement(6668..7591)
                     /locus_tag="ACHA34_002558"
     CDS             complement(6668..7591)
                     /locus_tag="ACHA34_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793147.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
BASE COUNT         2328 a         1560 c         1198 g         2840 t
ORIGIN      
        1 tataataaac atttccttga ggtttccttt taatcccctt cggaaaatca ccttttgtaa
       61 aatcgctctt aaggaaatca tctaaattac cagttctggt ctttttacct ccagtattcc
      121 ctgatttttt aggtggtaca tgttctttag taagttcagt gtatttttca cataaaatac
      181 acttacccat ctttttcata tctatcccca ctctttttaa ttcttatata caattatatt
      241 tcaaatattc attaataaat atccctattt atgataagga cctccctaac tacttctcta
      301 acgatttcaa atcgctagag aagcgttagc gcccctctaa cgttgtttga tataacctag
      361 actagatcgt taacgtaaag ggtgttttta acacaaaaat agccgtatcc tttgttttgg
      421 atacggctat ttgatattgc tcattcaatt gttgtaccac ggcatgcgtg aatttcatcg
      481 taatttccaa catctcctta tggttaggat caaaggttat tttctctata aaggtacgat
      541 acaaggcttt gatttgcttc ttctcactct gggcaagaaa acgatccaaa ttagtcaata
      601 tccgttggac atctttgact tgaatcggct ctccttgttg aaagatccct aagatttcat
      661 tttctcgaat gtgcaactgt cgtcgttttt cagtcaattc gtctaagcgg tcttttaaca
      721 tcggaaacaa ttcaggacta tcttccaaag cagcttccca tttttcaatt ttagtcttca
      781 attcttcctt tcgttccagt aacaccacga gttcttgttc caatggttgg ctttgtcgca
      841 ctatttcttc atttaacgct gccaccaatc ggggaagaac ttctggtagc tgaacgatct
      901 cctttaatcg gtcggcgaca aatgtttccg cttgttcggc acgaatcgaa ttggcatgac
      961 acacacttgc tcctttattc cgaaagactg aacaagaata atatcgaatc cgtttctttg
     1021 tgccatcctt tagggtattt gtgacattac tcgctgccat cggtgcgcca cattctggac
     1081 aacgtaacaa gcctgtcaat acattttcac ctgtatggtt ccatttgggt tgttttcctt
     1141 ctaaagcaag gcgttcttgt actttttgat aatcttcttc cgaaataatc ggttcatggg
     1201 ttcctttcac taaaatcggt ctcggattct tccccttccg tcgtttggtt tcccaattga
     1261 tgtagcgcgc atattctaaa taccctgcat aaattttatt gcgtaagata tctttgactg
     1321 ccgtggtaga aaaagcattc tttttgactg tttgatatcc ttgacgattc aaaaaattgg
     1381 caatcgctcg ataccctgct ccgcctaaat attttgcata gatcagacga acaattttcg
     1441 cctcttctgg ttcaatgacc aattgttttt gaccattcgt gtcaatacca ctgacatagc
     1501 ctaaacgtcg gcctcctgca taacgctttt tatctttcac taagctttgc attgacattt
     1561 gaacattctc cgcaatttgg ttgcgttcaa attcaccgaa actcccaagg agttgtaaca
     1621 tcaatttacc tgtcgaagtg gtcacgtcaa attgttccga gatcgaatgt aatcctacgt
     1681 catatcgcaa taattcctcg actgtttgta agacatctaa catattgcgt ccgagacggg
     1741 tcaatttcca aatcactagt agatcaaaac gatggttccg agcatcgttc atcattcgtt
     1801 gataggcgaa ccgatttttt gtggatttcc cactgattcc ttcatcggca taaacttcta
     1861 ccacttcata gcccatccgt tcggcaaact gtcgtccatt ttgttcttgt gcttgaatac
     1921 tatgtccttc taaagcttgt tcttgtgtac tcacgcgaat atacaatgcg actcgtttgg
     1981 tttgtatcat ttccatagat cattcgttcc tttctaaaat attttgtgtc agttgtcgga
     2041 agtaaatccc acaaactgtt ttatcctttg ttacctcaat ccgacaaaca tagtcacata
     2101 ataattgtcg actagagagg tcttttagtt gaagccattc tttcactttt gcttcacact
     2161 gttggagtga aagaaacgaa gaagtagaca tcggcttttg aagagcttgc agttgttgct
     2221 tgagttcttc cagtgaacaa ttgccctctt caaatgcctt aatccattgt gcttcttgtt
     2281 tcttttgttg gattactttg cgtttttcat cttttcgctt ttcggctaat tgttgcgtta
     2341 attgggcaat caaggcttga tagacgcctt cgtattgaaa aaaagcctgt atcttttcca
     2401 atacttctga ctcaaccctt tctgcccgaa gaaaaattcc ttggcagctt cggtcggtac
     2461 aatgataata atgaagcgtc ttcgtcttct ttgtattcca ttgatacttc gaactcagat
     2521 gacgaccaca gaagggacac cgaatttttt gacgtaataa gtacagatac gtatcttttt
     2581 tagctgtaca ccttgattgg cgaatctctt gggcttgtaa aaaggttgct ttcgagagaa
     2641 tcggttcaaa ggttcctaga tacttcccca aagatcctcc tttgatttcg ccatagtaca
     2701 atggattttt taggatacta taaatatgat agggctgaaa aggacgttcg cggaagaaaa
     2761 gcttttgttg cgtcaatcgt tgacaaagct ttttgtagcc atattttttg gtcaaataaa
     2821 gttgaaaaat atagcgaaca atccttgctt cgtgtacttc ttgttggatt ttcccattca
     2881 ctaaacgata cccatatggt accgcagaag acaaatattg ccgttgttgc tttttatagg
     2941 aaagtccggc acgaacattc tcaataatga cgtttcgttg ttgttctgca aaagtgaaca
     3001 ataattgcac aaatagttta ttcgttgacg cgtgtgagaa agaagcagga agaggttcag
     3061 aaatcgaata cacttgaacc tgatgttgtt ggcacaactc aataaattct aaggctattt
     3121 tggcttgacg tgccaatcga tcataacgcc aaacatagat caccccctct tcttctgacg
     3181 cacaaaccca agctctaagc tgtcgaaaag ctggacgctc cgctttttcc cctgattctc
     3241 ctttgtctga aaatcgttgg ggaatttgtt catatttctc taaaacatac tctttcactg
     3301 catcatattg agcgtcatta ctaattttcg cttcttcata aacagacgtt cgatcataac
     3361 aagccacacg catgcttgat tcccctctct atgaatgaac taaataccat tttttcacga
     3421 ctcttctatt gaacgaacgt tgcacaaagt gattttctaa atatccttcg ttcttcaacg
     3481 cttgaagagt acccccgata gatccagcag atacccctct tatttcgaaa tccaagtaaa
     3541 ttggttttgt aaaaaaggct tgatttgaaa aatgttcttg aatatacaga agaacctctg
     3601 cttttagtgt cattttattt ttagtcatta gaattttcct ttctttcatg tttgattgaa
     3661 tgaagtaaat gaagcataga tcaagcttca tttacttccg tttgggtttg ctcgctatca
     3721 tacagataag taatcagttg ttttgctaaa tcaataagaa atatttccat aacatggtgc
     3781 ctcactttca tactttggtt tattctaaat tacaatcgaa aatacgattt gagtgaagaa
     3841 ggtaatagaa tctcgtatgt tggaagaact tgacgattca tgtgaatacg tttcgttctt
     3901 ctcccttttt ctgcacacat attacccgaa tcaagcaatt gcctaaaaag ataggttaga
     3961 tcctttactt gaaattctcg ccttaacaag tcctcaaacg ggataggaaa aatatttacc
     4021 ttaatatccc catttttttg aattgatact tttccccaaa tcgtactggg cgtatgtttg
     4081 ttgtcactgt ttgttaaaaa tttatggcta ttcatcacca acaatgtttt cactcggtca
     4141 aataccgttt gctcacttcc taaactagta tctacaatta actgttcgta cgaattaata
     4201 agcaccgtct gtatatgctc taatcgtaaa ggatagccca gtatatcttg aattaggagg
     4261 gctccactat aaatcaaagc tagattatta ccaatgcgtt gcttcaaaga atgttcaggt
     4321 aaaacagccc atagtttttt ttgagcttcg acaaaagttg cttcaatttt tgtaatccct
     4381 tgttcaaaga gtttcaaaag aaataacttt ataccccaac caaaagtatt agaagaaaga
     4441 cttttaactt tctctgcgtg attcgcatcc tttgtaaact tgatatcaga aaattcgata
     4501 tatcgaactt tcaaaccagt taatttctca ttgaaataat tttccaaacg ctcttcagaa
     4561 gaggtaaggg tcaaaacaga aaaagtctct tgtggcataa gctgacaatt agaatctaat
     4621 cttagccttt cttctcctga agctaattga tagatcaacg agctaaagtt attttgagtt
     4681 gctgctccta actcatctag tattagtgga acacctccct gattgtttga tgctagcttt
     4741 ataatggctt tattggtagc attcatattt ctaagaaccg tagttggatt tccaaataaa
     4801 gaggcaataa agtgtaacgt agtggtttta cccgttgtgc tcttgccctg taggttaatt
     4861 agtaaactca aaaaagtatt agccttcgta acgtttaaat agcctaacac aatagaagca
     4921 ataccaatcg accaagcaag ttctaagttc gttcctaaaa catacttatg aaccaatgcg
     4981 tcaaattccg cttctgtacc attagttgtt aattggtagc atgagttttt agttaataca
     5041 gaaggaattt cacgtccaaa agaatcttgt aactgaaaaa tcattgcttc attttctttt
     5101 ttccagccaa gagattcatg ttcaaaaaag attggagcat tctcccgttg aatctgtaaa
     5161 aagtcaacga cttcagcttc tcttctagct ttaatgatta agccatgttt tgctaattgt
     5221 ttaaaattat tagtttgtaa acatccacct tctatttcta tttcatccca acaatgatcg
     5281 acataatagg caagaacaag tgtaatgact ttttgttcga tatttttgcg tatttgctta
     5341 ataaacatcg gacaagcaac tttttcttct tgaccttctt tcaccaaaaa tactccttct
     5401 tgattcagtt taaactggtc aaaataatat tcttgattgg attctaatga ttctacagaa
     5461 ggatttacag gttttggcaa cggatatttt tttacaaagt cttgaatatc tttattttta
     5521 tccgaagcat gcagacaatg aaatcgaatc ttattatcag ccgtaacaga caaacaagaa
     5581 ccattagaat tattcgtgtg ttttcttaat ggacattcgt caaatataag taaggtaact
     5641 cctttatgat cgccctcttt tatggtataa gttaattcag gatagtgagc taaccattgt
     5701 ttatagtctg cttgaataaa aggtaacact tctctaaaag atggatggct ttttactcta
     5761 gaggttgaca tcatggaatt ttcttcaact atctgttcaa gaattttaaa acaattactt
     5821 ctatctatgt tatcgggaat agaaaggaaa ctagataaac gatgcggacg ctcttttgtg
     5881 gactttcctt tgaccgctgg tgtaccaact aatttgcata atcttgaagg attataaaca
     5941 gaagtatcaa tatcaacttc atcttctact ccaaatcttc ggtgaagtaa ttgaagaaaa
     6001 ttctgaatag taagaatcgt ctcgtgatta tttggttgtt catttatggg aagaaaaata
     6061 tggtagccat tcccactatc aacttttaca aatgattcta ttctcttttc tttcaaataa
     6121 cgttctactt gttccataac attttctgcc tttttctttt cgctatctgt tgcacttccc
     6181 tctttccttt tagggtcaat atcaataaat acaaaccgaa ttttttcaat atcattgtct
     6241 ctcacagaat gctttttttg tgtaatcacc tcttgtttaa taggattata agttatgttt
     6301 actgtacctt cagacaattt ctgtttttct aaatagttaa taattgcttg agaatctgca
     6361 gtagaaaaaa tatgagaaac ggttttcttg tttaatgtag aaaaagttct caaattaaca
     6421 taagaaatcc ccagtgtatt taacagttgt aaatgcttct gaattgaagt gtgattcatt
     6481 ttcattaatt accactcact ttctaaaaaa tttttatttg tatgactaca ttacctccaa
     6541 cctctcataa tttcatgatc ttcagttcta caaaaatata tcccactcaa aatcttgaca
     6601 aaagaaaaaa gttgtgattt tggaaatcac aacttttttc ttttacgata aatcaatcct
     6661 tattaaatta tttaggaaaa tattgttcaa ttatttcttt atatattttc tcatagtatg
     6721 tttctgtcca tcctaatgtt cctataaaat caagtcctct acattttaaa ggttcactta
     6781 aactattaat atagttaata atgttgtcta tccttttctc tttatcttca ccttcaatct
     6841 ggggagcata tagtccaatt tcctcccaat aatatgaatc tctttcaatt cccatgatgg
     6901 tagctttggc taatttcaaa tcaaataacg attttagaat tcttagtgtc tcaataaagt
     6961 cttctctctt caactcttta atccattgtg aattttctgg attatctaaa atttcaagtt
     7021 catcttcatt ataagtatat ttcaaatttg acttgcttaa ctcatagata aatgaagttt
     7081 cttgactcat aattctttta agtacttgac tataagtttc gtttgacttt gcccatttcg
     7141 ctaattctac cactgtattc actaataaat cgaaataatg atgttctcgt tcaatagtct
     7201 cagcattttg aaaatgaaaa tcacctttat aaactccatt cttttccact gttttattaa
     7261 tattttttaa aaaccagaat gagtttgcaa atttagtcgt actttttcga ttaactaagt
     7321 taataactct tccatgtaat aataagacaa aaatacataa atttttagct tccttattat
     7381 tagatactaa ttgatcaatc atagggagcc attttttgat tttcactaat tgattgctca
     7441 taaatctttc actggacttt tgttcaatct taaattgcaa aacttctaac tcataattcc
     7501 catcttctga tttatttttg ggaacaacta tcaaactttt ataaagttta tccacatcaa
     7561 aatttatctt ttttgcataa tttgtagcca ttaatacatc tccttattca taataaattt
     7621 catatgacgc tcctctcaaa atttaccggt agactagaaa aaagaataaa agatacgtca
     7681 attcacactt tattcttaaa aaaaaactgg tctctctacc ggtaatatcg attgagtccg
     7741 cctttatcga ctttatcctt tttttatatt aaattttttc accggtagac agggagagaa
     7801 agaataaact aaaaagccct taattcttaa tgttttcacc tccttaccgt tcctaccgct
     7861 aaaatttaat ataactaaat tctataacat tagaagaaat tttaaatacc tttgataaaa
     7921 aatatt
//
LOCUS       DAYYFU010000092         7777 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000092 DAYYFU010000000
VERSION     DAYYFU010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.097"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..179)
                     /locus_tag="ACHA34_002559"
     CDS             complement(<1..179)
                     /locus_tag="ACHA34_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFM8793148.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
     gene            complement(192..1295)
                     /locus_tag="ACHA34_002560"
     CDS             complement(192..1295)
                     /locus_tag="ACHA34_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM8793149.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1385..2320)
                     /gene="pplA"
                     /locus_tag="ACHA34_002561"
     CDS             complement(1385..2320)
                     /gene="pplA"
                     /locus_tag="ACHA34_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFM8793150.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2622..4556
                     /locus_tag="ACHA34_002562"
     CDS             2622..4556
                     /locus_tag="ACHA34_002562"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8793151.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5013..5429
                     /locus_tag="ACHA34_002563"
     CDS             5013..5429
                     /locus_tag="ACHA34_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM8793152.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5443..6009
                     /locus_tag="ACHA34_002564"
     CDS             5443..6009
                     /locus_tag="ACHA34_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFM8793153.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6032..7018
                     /locus_tag="ACHA34_002565"
     CDS             6032..7018
                     /locus_tag="ACHA34_002565"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8793154.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(7173..>7777)
                     /gene="scm"
                     /locus_tag="ACHA34_002566"
     CDS             complement(7173..>7777)
                     /gene="scm"
                     /locus_tag="ACHA34_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM8793155.1"
                     /translation="TTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDST
                     DTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTD
                     TSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNK
                     SKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2215 a         1489 c         1616 g         2457 t
ORIGIN      
        1 ttattgatag cggtagtcgc taaatcaaaa actagcattc caataaaaaa taaaaaagtg
       61 tttagccaat gtatctcatg aaaaaaagcg atagagaata atacccctac cgcaaaagga
      121 aatacactag ccacctttgt tcgtatttca actacttcta aaaagatttt tagtgtcatg
      181 gttcgtactc cttattttag ttcagaacgg tcacccatcg tgtaatggga gtcctccgat
      241 aatttgaagg tgtctttgat tggatcggtc acatatactt tatcgtcttt tgtcacgaag
      301 atcgcttccg tcccgttatt caattcgctt tcgacgtatt ccagtccttt tttcacaccc
      361 attgaaaaga ctgctgtaga caacccgtca ccatcaattg atttatctgt tatgatcgtg
      421 acacttgcga tatcattgtc gaacggataa cccgtttttg gatcaaacaa atgatggtat
      481 tttttgccat ctacttctaa ataacgctcg taaattccag aggtaactaa tgttttgttc
      541 cgttctttaa tagaacctaa aacagatccg cgagccatgt tcggatcttg gatgccgact
      601 gtccagtctt ggttttcgcc tcttgggctg tggcctaaga catagacatt accgcccaag
      661 tcaacgatgg cagtggtcac tccttgtttt ttcaagactt tcactacttc gtccgtaatg
      721 taacctttgg cgattgcacc taaatcgatg atcattcctt tttcttttag ataaacagtt
      781 ttttctttgt catttagttc gatcttatga taatccacta atttcaatgc ctgatcgatt
      841 tcttcttggg aaggttttct ggcgtcatca aaaccgattc gccataattg tgtgattgca
      901 ccaatcgcca tatcgaatcc gccttgagaa tcttggctat attcgtaggc ccgcttgact
      961 aacgtgtaga cgtcatcaga aacttttact ggtttgatcc cagcttgctc attcacttca
     1021 tcgatctcag aacctttttg attgatcgtg attttatcac caagttcttt tactcgatcg
     1081 aaggcaggtt tcaatgctga ttctttgcct tcatcataca cacggatacg aacgtaagtc
     1141 cctaacaaga actgttcatc agaatatgga tctttcaata tcttggtagc tgctgaactt
     1201 tcagtggttg ttttatttgc tgagttgctg cagccagtta gcaccataaa agagatgccc
     1261 agtgtgatta aggaaaatag tagttttttc cgcatctttt tcctctcttc attttgaaag
     1321 gtgagacgac tgcctaatct ggctatcgtc ccaccctcca acttttatta gtatgattat
     1381 gtgattattt ttctgttacg atattatcta cttcgattgt ttgagtgtcg cctttttcag
     1441 ctgcattcac taattgttgt gcgtacatga tgaatgaatg tgagctgtgt gtagcacctg
     1501 taacaacttc tacattagca ggtgagccat cttcttcgcc cattgctttg acgaattcgt
     1561 cgttcagagt agggatgtaa gtttttggtt ctgtaccgct ttttgctttc atattttcat
     1621 tatattctgt atcatcttgt tttgatttac cgtctttgtc gatataatca aagtttgatt
     1681 cagttacttt gccgccagca actgtcattt cgaattgcac acggtagccg ttagagtagt
     1741 tcttttcttc aagcttgtaa gtgccgtctt tcaaatcagc accattgtcg atttcgattg
     1801 tatctgtgtt accagcttga gcagcttgga tcaattgttg tgcgtagttt tggaagcttt
     1861 cagatgagtg agtagctcca gttacgactt ccacaccgct tgcgctttgt gcttttaaga
     1921 attggtcatt caactcagga atgtattttt ctgggtttgt gccagatttt gcttccatgt
     1981 ttttgttgta ttctgcatct tttgtttttg attcgccgtt ttcattgacg ttatcatatt
     2041 ttgattcagt gattttgccg tcttttacaa ccatttcaaa gactgcacgg taaccatttg
     2101 aataattttt ttcttctaat ttatatgtgc cgtcttgcaa atctccgcca gctacaactt
     2161 ttgcagatga ttctgttgtg ctttttgcag tcgaagattg tgctgtagat gattcagaag
     2221 aagagtttga gcttccgttg tcatctgcgc cgcaagcgcc taacactaga gctgaaaatg
     2281 ctaatacggc aaatccagtt acaaaacgtt ttttcttcat tttaataccc caccattcca
     2341 tttctagtcc acaatttttc tttgtgattc attatacatt ctaatctttt tagcctataa
     2401 gtcaactatt tttgataaat aacccatgta atatttcaaa cgttttcttt cagcaaattt
     2461 cgtgaaaaaa cgctttattt taaaatttga aaaaaatcca aaaggagtta gacaaaagaa
     2521 cagaacatct atataatata tggagattgt gtaataagta tcaaactaaa agaattatca
     2581 tatattcatt atatagatag tctataagga gcggatgatc aatggcaaag caaaatattg
     2641 tcgttgtagg agcaggatat gccggtgttt cagctacaaa atacttggca aagaaactga
     2701 aaaaagaaga tgtagcaatt actctgatcg atcgtcattc gtaccatact atgatgaccg
     2761 agttgcatga agtagctggc ggacgtgtag aaccagaagc aatccaatac gatctgcagc
     2821 atttgtttgc tcgtcaaaag aatgtacaac ttgtcacaga cactgtaatc ggtatcgata
     2881 aagaacgaaa aatcgtcaag accaaattag gttcctatcc ttttgatcag ctgatccttg
     2941 gaatgggcgg agagccgaat gactttggta cacctggtgt caaagcacac ggattcaccc
     3001 tttggtcatt tgaagattct gtaaggattc gggaacatat tgagaagaca gtagcaaaag
     3061 ctgcattgga accagatgat gaactgcgta aagccatgct gactttcgtt gtctgtggtt
     3121 ctggatttac cggtattgaa atgattgggg aactgatcga ctggaaaaat cgcctagcca
     3181 aagattataa attagatcca aacgaaatca cactgatggt cgtagaggca atgccgacga
     3241 tcttgaatat gttggatcga aatgatgctg caaaagctga acgctatttg cgtaagcaca
     3301 acgtggaatt attattgaat gcaccaatcg tagaagtggc tgctgatcat atcaaattga
     3361 aagacggacg caacattccg actcacacac tgatttggac agctggtgtc aaaggaactt
     3421 ctgacgctgc tgactttgga ttagaatcag cacgcggaca gcggttgatc gccaatgaat
     3481 atatgcaggc aaaaggctac gaagataaaa acatctatat cattggtgat ttagtctttt
     3541 atgaagaatt cccaggaaca ccgacaccgc agatcgttca agctgctgaa caaacaggac
     3601 acacagccgc agcgaatatc gtggcttcga tcaaaggcgg agaaaagcat aaatttaaag
     3661 gaaactatca aggatttatg gtttctattg gtgccaaatg gggggtagct aacctatttg
     3721 ataagatcca tttaagcgga tttttagcta tcgtaatgaa acatatcgtg aatttgaaat
     3781 acttctttga tattcgttct ggttattata tgttccaata tttaatgcat gagtttttcc
     3841 atatcaaaga tgatcgcaac gtgactcgag gacattcttc tcgttacggc aatgtcttat
     3901 ggagtgtgcc acttcgtatt ttctatggtt tagtttggtt cgttgaagct tccaaaaaaa
     3961 tcattgggaa tggtgaagtg ttcaaaccaa gtacttggtt tggtgaagga tcatggttca
     4021 ctggaaacag tgtgttccct tggacatggg aatacaatgt aacagatgtt gccagtggtg
     4081 cttctgctgc tggtgatgca accacagctg ccagcggagc aggggcaagt gccggagaag
     4141 cagcgactca agctgcacat tttggtttga gttatgcata cggtgaagaa ccaatggctg
     4201 tgattgagaa gtcaccagac tggttcacaa agatcatgca agtgatcatg ccaaacaatg
     4261 atgtagcaat gttcttccaa aaattcatgg tattctttga aattgccttg gcattagcat
     4321 tgattgctgg attattcaca tggctatgca gtgcgacaac gattgtattg gtgatcacgt
     4381 tctgcttatc aggcatgttt tactgggtaa acatttggtt catttttgtt gctttcgcat
     4441 tgatgaacgg ttccggacgt gcgattgggt tggatcgctg ggtaattcca tggctacaac
     4501 gtacactagg taattggtgg tacggtagac caagttccca gtacggcaag aaataaatag
     4561 ataaagaacg ttatttgaat gcaagatttc aatagaaagg ggctgagaca aaccgtcaca
     4621 gcctcctttt tatgtaaaat taaaggaata agatgcccga gataagcctc catgagcaag
     4681 acacaggtgt cacaacctct gttacaacct cttgttataa acggtgttcc agaagtagct
     4741 tcttcgaaaa ataagccgaa atttcacaaa aacttgaaaa gtaattttcg taaattcctt
     4801 cttatttctt gaagttgccc acttctgtca caacctcttg ttataaacgg tgttccacct
     4861 gctgcttctc ggtaataagc ccaaagaatt agaaaacatg gaaagctgtt ttctattctt
     4921 cgttcttatt actcaaagca agacacaggt gtcacaacct cttgttttta tgctaaaatt
     4981 gggtgagtta atctggaaaa aggagaacaa aaatgagcct taaagaattt gctgaaaaaa
     5041 gtctgctcaa accttgggat gtttttatta ttttgctttt gactgttctt tcttttttac
     5101 cagtcatgat ttttggtctt cagcaaaaca ccgcaacagc agaaaaagaa gcggtactgc
     5161 gtgtggatgg cacagaaatc aaaacattcc ctttgatggc tggagaagag agcaacacat
     5221 atacttacaa agatgaacac ggagattata acttgatcga gatagatggc gatcgagtac
     5281 ggataaaaga agcagattgc gatgaccaga tctgcgtacg tcgaggctgg gcaacgaaaa
     5341 atggcgaaac catcgtctgt ttgccacata aattagtgat cgaagtccgt tcaacagatg
     5401 gaggtgacga agacagtctg atttattaga ccttcacaca ccatgagtaa attacaaaaa
     5461 atgatttata tttcgctact tgttgcacaa ggagtgatca ttggtttatt ggaaaatatg
     5521 atcccttatc ctttttcttt tgcgcctggt gcaaaactag gcttagccaa tctaatcacg
     5581 atcgttgcca tttttaccat gaaaaaacaa gacagcttct tactactttg gctacgacta
     5641 ttcctgacta ctttgctagg cggaacgatt tccacattcc tttatagtat gagtggtgct
     5701 ttgcttagtt attttggaat gttattggtc aagcaattag gaccaaaacg tgtcagtatc
     5761 atcggaatta gtgcggccgg cggtttcatg cataatgtcg gacagctttt gatggcttct
     5821 ttcattgcac aatcatggac agtcatgttg tacttgccag tcctttcagt tttagggatt
     5881 ttatcaggga ttgcaatcgg gatcgcagcg aattatcttt tacgacatgt gcagacactt
     5941 caacgattcc aaaaagatta tgagcaggga accaaacaaa aatggagccc aatgtttacc
     6001 aaaaaataat cgtcacacaa ggagtttttt tatgaagcta catccaatgt gggaaaacta
     6061 tcctgcttta agtaaagaat tgacagaaac actaaaacgg atggaagaaa ctgtccgcct
     6121 gaaaaacaaa cctgtcgaac aagccatcaa agaaacgatc catgccggcg gaaagctgtt
     6181 gcgtcctgcc tatcagctat tgttctcaca atttggacca gagcaagatc gagacaaagc
     6241 catcgctttg gcagcttcta tcgaaatgct ccatacggcc acactgatcc atgatgatat
     6301 cgttgatgaa gcggatctgc gacgtggtca gcctaatatc cgtagtcagt ttggtaatgt
     6361 cgtttctgtc tatgcaggag actatttatt tgtctgctgt tttaaactat tgtctgatta
     6421 ctccacttcc ttgaaaagtc tccaattgaa ttctcgcagt atggaaaaag tattgaatgg
     6481 cgaattaggg cagatggatg atcgctataa ttatgaactt tctgtaaaag aatatttgga
     6541 aaatatttca ggtaagacag ctgaactgtt ccaattaagt tgttctgttg gcgcttttga
     6601 aagtggaaca agcgagcgct tcgcaaaaaa agcaggagat atcggacttt ctatcgggat
     6661 ggcctttcag attatcgatg atattttaga ttatacaaaa gagagccagg aaatcggcaa
     6721 acctgtacta gaagacatgc gtcaaggggt ctattcttta ccgttgatct actctttgca
     6781 aaaaaacaaa ccaaagctac taccttattt agagaagaaa gcggcaatga cagaagaaga
     6841 tgtcgacgcc gtacgcaaaa tcgttgaaca tacaggaggc gtggaagaag ctaggaagtt
     6901 agcggcctct tacacagaaa aggctttgaa ggaaataaag aaattacctg ccacctctct
     6961 tcgaacaaaa gagaatctct tttcccttac ccaactgatt ttggatcgca aagactgaat
     7021 agggaaaggt tcgatacgta aataaatttc tcagaaggaa atctacgtgt tttttgacaa
     7081 attgaattgt agttcttgta tttatgataa aaagcaaaaa taaagagcaa cgtccgttag
     7141 cgacgttgct cttttttatc ttgaatattt ttttaccttt tcgatttctt ataactcata
     7201 aaagtgattc ttaaaccgat tacaagtaat ataacgcctg cgagtgttat ccaattattc
     7261 gattgacttc cagttttagg aagtgctctt tttgatttgt ttccattgtc attgcttgtt
     7321 ttattagtta agttaaagtt cgtcctgtta tccgaacttg tttttgagaa ggagacactt
     7381 gcatgacgtg tatcgcttgt ggtagacgac gtgtctgtac tatcgcttga agttgatgaa
     7441 tctgagctat cacttgtggt agaggatgta tctgtactgt cacttgaagc tgacgtgtcc
     7501 gtgctatcac ttgtagtggt agatgaatct gagctatcac ttgtggtaga ggtagtatct
     7561 gtactgtcac ttgaagctga cgtgtccgtg ctatcacttg ttgtggtaga tgtatccgta
     7621 ctgtcgcttg aagctgacgt gtccgtgcta tcacttgtgg tagaggtagt atctgtactg
     7681 tcacttgaag ctgatgtgtc cgtgctatca cttgtggtag aggtagtatc tgtactgtcg
     7741 cttgaagctg acgtgtccgt gctgtcactt gtggtag
//
LOCUS       DAYYFU010000093         7760 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000093 DAYYFU010000000
VERSION     DAYYFU010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.098"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="ACHA34_002567"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="ACHA34_002567"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFM8793156.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3285)
                     /locus_tag="ACHA34_002568"
     CDS             complement(2032..3285)
                     /locus_tag="ACHA34_002568"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFM8793157.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="ACHA34_002569"
     CDS             complement(3291..4214)
                     /locus_tag="ACHA34_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFM8793158.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="ACHA34_002570"
     CDS             complement(4435..6927)
                     /locus_tag="ACHA34_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFM8793159.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="ACHA34_002571"
     CDS             complement(6934..7401)
                     /locus_tag="ACHA34_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFM8793160.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7537..7610)
                     /locus_tag="ACHA34_002572"
     tRNA            complement(7537..7610)
                     /locus_tag="ACHA34_002572"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7574..7576),aa:Arg,seq:acg)
     gene            complement(7668..7741)
                     /locus_tag="ACHA34_002573"
     tRNA            complement(7668..7741)
                     /locus_tag="ACHA34_002573"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7705..7707),aa:Pro,seq:tgg)
BASE COUNT         2161 a         1691 c         1247 g         2661 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga gtcgaacccc
     7561 taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc atattcaatt
     7621 taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata aaaaaaatcg ggaagacagg
     7681 attcgaacct gcgacccctt ggtcccaaac caagtgctct accaagctga gctacttccc
     7741 gtttaaaaaa taaaaaagac
//
LOCUS       DAYYFU010000094         7601 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000094 DAYYFU010000000
VERSION     DAYYFU010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.099"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..428)
                     /locus_tag="ACHA34_002574"
     CDS             complement(138..428)
                     /locus_tag="ACHA34_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793161.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="ACHA34_002575"
     CDS             complement(546..806)
                     /locus_tag="ACHA34_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793162.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="ACHA34_002576"
     CDS             complement(943..1356)
                     /locus_tag="ACHA34_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFM8793163.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="ACHA34_002577"
     CDS             complement(1812..2297)
                     /locus_tag="ACHA34_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793164.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="ACHA34_002578"
     CDS             complement(2436..2651)
                     /locus_tag="ACHA34_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFM8793165.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="ACHA34_002579"
     CDS             2875..3675
                     /locus_tag="ACHA34_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8793166.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="ACHA34_002580"
     CDS             3694..5562
                     /locus_tag="ACHA34_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8793167.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="ACHA34_002581"
     CDS             5555..6046
                     /locus_tag="ACHA34_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFM8793168.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /locus_tag="ACHA34_002582"
     CDS             6034..6588
                     /locus_tag="ACHA34_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFM8793169.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..7601
                     /locus_tag="ACHA34_002583"
     CDS             6606..7601
                     /locus_tag="ACHA34_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFM8793170.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2709 a         1213 c         1442 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg a
//
LOCUS       DAYYFU010000095         6964 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000095 DAYYFU010000000
VERSION     DAYYFU010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.100"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            276..872
                     /locus_tag="ACHA34_002584"
     CDS             276..872
                     /locus_tag="ACHA34_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8793171.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(930..1412)
                     /locus_tag="ACHA34_002585"
     CDS             complement(930..1412)
                     /locus_tag="ACHA34_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793172.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1518..1715)
                     /locus_tag="ACHA34_002586"
     CDS             complement(1518..1715)
                     /locus_tag="ACHA34_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793173.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            2010..2768
                     /locus_tag="ACHA34_002587"
     CDS             2010..2768
                     /locus_tag="ACHA34_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM8793174.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            2828..3256
                     /locus_tag="ACHA34_002588"
     CDS             2828..3256
                     /locus_tag="ACHA34_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793175.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            3302..3374
                     /locus_tag="ACHA34_002589"
     tRNA            3302..3374
                     /locus_tag="ACHA34_002589"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3335..3337,aa:Gln,seq:ctg)
     gene            complement(3524..3895)
                     /locus_tag="ACHA34_002590"
     CDS             complement(3524..3895)
                     /locus_tag="ACHA34_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8793176.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            4110..4295
                     /locus_tag="ACHA34_002591"
     CDS             4110..4295
                     /locus_tag="ACHA34_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793177.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(4378..4566)
                     /locus_tag="ACHA34_002592"
     CDS             complement(4378..4566)
                     /locus_tag="ACHA34_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793178.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(4590..4853)
                     /locus_tag="ACHA34_002593"
     CDS             complement(4590..4853)
                     /locus_tag="ACHA34_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793179.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            complement(4841..4984)
                     /locus_tag="ACHA34_002594"
     CDS             complement(4841..4984)
                     /locus_tag="ACHA34_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793180.1"
                     /translation="MIQNNTLCEMAQAVLWIYPVKSVSILKLEEKLAIIKVKEGVGFS
                     WIM"
     gene            5011..5268
                     /locus_tag="ACHA34_002595"
     CDS             5011..5268
                     /locus_tag="ACHA34_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFM8793181.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(5339..5530)
                     /locus_tag="ACHA34_002596"
     CDS             complement(5339..5530)
                     /locus_tag="ACHA34_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793182.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(5671..5991)
                     /locus_tag="ACHA34_002597"
     CDS             complement(5671..5991)
                     /locus_tag="ACHA34_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793183.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(6014..6448)
                     /locus_tag="ACHA34_002598"
     CDS             complement(6014..6448)
                     /locus_tag="ACHA34_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8793184.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            6783..>6964
                     /locus_tag="ACHA34_002599"
     CDS             6783..>6964
                     /locus_tag="ACHA34_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8793185.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2178 a         1359 c         1068 g         2359 t
ORIGIN      
        1 aatttttaat ggcaatatca taaaaaaaag aagatagaga tttaataatt atatatcagc
       61 tgtttttttc aaataagcaa aaattcctaa aacacaacaa tgaaagcgat aactaagaaa
      121 atccttacat aacgattttt cttattaaaa ttgttttttg aacttcgctt attttttgtg
      181 agcatatctg tttacttact ttttgtaagg tgctatactt catttcgtaa tcaattaaaa
      241 aaatgcaaaa cgcaaatcaa aggagaaatt ttattatgac aacttttact gacactttga
      301 aaaaccgccg ttcaatctat catctaggac gtaatgtttc actttctaat gaagaattaa
      361 caactttgat caaagaagca attaaagaaa gcccaactgc tttcaatgct caatcaacac
      421 gcgcagttat ccttttcgga gatgcacatg aaaaactttg ggaaatcact gaagaagcac
      481 ttcgcccatt gactccagca gaagctttcc caaatacaca aaacaaatta gctggtttca
      541 aaaatggtta tggtacagta ttattcttca aagatactga cgtggtaaaa ggtctacaag
      601 aacaatttga attatatgca gacaacttcc cagattggtc agaacaatca aacggaatcg
      661 caacagctaa tacttgggta gcattagttg ataaaggatt gggcgcaaac ttgcaacatt
      721 acaacccagt tattgacgaa gcagttgcaa aagaatggaa tatcccttca aactggaaac
      781 tacgttcgca attagtattc ggttcaccag aaacaccggc tggggaaaaa gaatacatga
      841 acgatgccga tcgcttccgc gtatttggat aattttattt gaaataaaaa acagaggttg
      901 tgacaaacat cttgtcgcaa cctctgtttt tattctggat caatcataat atcaatctct
      961 gcctcatcgt ttaagcgttg ggacaaaaat ggaactaatt cttctaatga tgcagaaaat
     1021 ttcagcatcg gtcctcgcca tccgtttcgg aaaaaattca tttttacgat tttagaacct
     1081 tttggctgat ataatacaat tttattgatc tcagaaaaag gaacaccttt tttgtcaaag
     1141 ctattcaaaa caagtccttc ttctgtgatt cctctactgt ccaacaaaaa actaataaaa
     1201 atcattgctg acaagatccc ttttgccaat tcatctgctg taggatgact gatgagtgta
     1261 taccaaatga ctagtgacac taagaagggt atcaccaagt agacccatcg atttcgcaca
     1321 gtccgataaa taatttcttt tcgaaccagc caaagaaata aaaacatcac aacaacgaat
     1381 aaaaacaata gaaccgtaaa gaatgttacc atgacaactc gcctcgcttt ttcttctcca
     1441 gaaaatagga aaagaatact gatttacctt ctcaaagaaa ttggacttgc agcacgccgc
     1501 acgtccaatt gttttcttta ttcaaatgta tcatattttg caacaaccat gcttgctttt
     1561 tcatctaata attcttggtc ctctgcagca cattgggcaa ctttgaccac ggcgcagttt
     1621 gtcatttttg atacaacttc accaatgacc gtttttgtaa aaccaattgg tttgcatgca
     1681 tattgttctc ctaaattaat catttcttcc gtcatcaaaa tcctcctaaa ctgctggaac
     1741 ttttctccac attaccactg ctatttcttt ggagatttaa ctcattgatt tttatttaca
     1801 gttaaaaatc aaccttacag gcattatagc gtactgtttt cgttttcaca attaaaacag
     1861 ctgttgtatt tagaagaatt tttttcaaaa aattaaaaca tattccttaa aaactaatta
     1921 tttcctacat attaaaatat aatacttcca tttccaataa aaatacattt ataataggaa
     1981 agataaacag ctgaaaaggg agatttcaaa tgaagaaaaa aacgttcaaa aaactattgt
     2041 ttctaggggg cacagctgct cttttattaa gcggtacaag ttattaccta catatccatt
     2101 ctccagaaca tacaatgaac actgcacaaa aaagttcaac acaaaaaacg aaaaagacaa
     2161 aagagaaagc agaagtttta ctagatgttc cattagagag ccagtttgat tctccttctt
     2221 tagaaaatgg ctgtgaagtc acttcgcttt ctatgctttt atctttttat ggttatgaaa
     2281 caactaaaaa ccagttagct gagcaattgg attacgtccc tgttttcaat gcagatggga
     2341 ctcatggcga tcctaatgaa ggatttgttg gtgatatcag tggcggagac tgggctatgg
     2401 tcgtctatgt tcccccagtt gcctcccttg ctcagcaaat cgtccaaacg gattatcaca
     2461 cttttccaaa aaccgatgct caactcgatg atatcaaaaa agcactatct aaaggaaatc
     2521 ctgtctggac ttctgtgact atcaattttg aagtcccaga tgaaactgat tttatgactt
     2581 ggaccacaaa taatggagaa gtaagagtca ctcctcttgt tcatgcttgt gtagtcactg
     2641 gatatgacaa agaaaatatt tatgtcaacg atccctatgg tgtaaaaaat cgagcagtac
     2701 cgatcaacga tttttcggcg attttcactg caatgggtgg acagatgcta actttggaga
     2761 aaagctagta attcaaacaa aaatccctta tactcaagat accaaacctt tgaagggagc
     2821 gtagataatg gaaaaattca cacataaaaa aatggatcca aatgaaattc cgattatttt
     2881 tgtccgtgac tgcaagggaa atgttcaagg taaagtctct attaatgagt ggaacgagcg
     2941 tcgcagacct gctaccctca atgaactgga aatcaaattg tatcgtcaat ctcttgttta
     3001 ttacgcagat caagagtacg agaaagccac cgatttattg aaatttctaa ttgctcgaac
     3061 agagtatact cgctttgaat atattgaacg tttagcaaac atctatcata ttatgaatga
     3121 acctgtcaaa gaatatcagt tattggacac cgtcttgtca gtagcagaac tgattgccct
     3181 tcctgctgga ttagaaaaga aactagtacg ccgcctttta cgagtaaagc aacaactatc
     3241 cgatcaagaa aaataacttg cgttttttta ggaaagtgct atgattattt tgttgaataa
     3301 atgggatgta gccaaactgg taaggcactt gactctgaat caagcaattg taggttcgaa
     3361 ccctgccatc ccaatttgat gtgcttgctg aaaaaatgaa ccccctgtaa aagaacaatc
     3421 tttttgcagg gggattattg tattattttt actacttgta tttagtttac ataattaaat
     3481 tatgaataat aaaatcgggc gtattcttaa tacaaaaact gatttaccca ataattgcta
     3541 atgctttctc cggaactttt tctctaggaa cttcctcata cgtttctatg tgtgctcctt
     3601 tattgctaat tttcaaataa tgattttctt ttaacacaga aagaccatta aattcaaccg
     3661 ttcgttcttt cccattggca tcagcagctt tctgcacata ccttgctgta ccatattcat
     3721 ttacttcctc tggtttttgt gtctttacat agacctctcc tttttcaact aatggattta
     3781 gctgatcaag tataccaggg atttctccat aacttccttc agtataatat ttcagtccaa
     3841 atagcaaaat accactaatc gccgccagtc caatcaaata tttaatcact ttcatctttc
     3901 tacctccttg ttatatattc agtatatggg tcagttaaag aaattccatc cgtcccaagt
     3961 tggagatttt tctcctactt tagaagcgag aatcgagtgt ttttttagta aaaatgatcg
     4021 ttatttgata ttattgacgt gtggataaat cagtttttct cgttttgtcc atgtaaaaaa
     4081 tcatgttgat agcaaaggat gtgttcaaaa tgaatcaaga agaattattt caaaaagtca
     4141 aggaaatgat taagaacggc aatttcgatg gcgcaaaacg atttatcgaa gaacataaag
     4201 aacaattagg accttataaa gagaaagccc aaaatctttt aaaagatgta aatatcgaca
     4261 gtgtcaaaaa caaatttaaa aatctattca aataagtaaa actttcactt ttgtaaaatc
     4321 aaaggaggac aatgctgctg catcgtcctc ctttggttct tttctgaata cttcatacta
     4381 aaataaacgt gtcggatcgt aatgataagt cacattgccg ttcagtgagg cttttcccac
     4441 cacatattta cgattctcca acttcagcca cgcttctcga aaagccatta attcttctct
     4501 ttttacatca atcttttcga tttttccatc gatcaaatct tgaataagtt gttcatatgg
     4561 attcattttt ttcctccatc tgctgtcaat caataagttg taaaaatagg ttcatatgct
     4621 tttttggtat ctagatcttc tctgactttg actgtcgtat aaataatttt ttctactggg
     4681 atactataat aacttccttc accattattg aatcggtgaa cgtctttctt tgctaaaata
     4741 ccatttattt tattcgtcag tacacgttgt tcgacactgt tacttacaac atagtaaatc
     4801 acccgaatat tattgatcaa atgcaaatcg atttctaaca ctacatgatc catgaaaatc
     4861 ccactccttc tttcactttg attatagcaa gtttctcctc taatttcaaa atcgatacag
     4921 attttaccgg ataaatccac aaaactgctt gcgccatttc gcacaaagtg ttattttgta
     4981 tcatggactt tatttatcgg aggcaatagt atgatcgcga tcggtcaatt tgttttttat
     5041 attccttttt ttattatgat aagcattctt ttctattaca taaaatggac aaaaaagaag
     5101 ttttcagttt tactggcctc tttacctgct gtatatttta cttatcaaat tttttctttt
     5161 aggcattggg aaacaacgtc tgtattaata acacacatca tcgaattgac gctttcagta
     5221 atttttctta ttatttggat ctatttttta tataagaatc agaattgaat ccctcgcatg
     5281 tttaaaaaga aaacgattaa agatgcagca aatttctgcc gatctttaat cgttttttct
     5341 attctgttat accaaaaaaa tgtttgaatc ggtgaaggat cgttccttgt tttaattcta
     5401 taaattcttg tgcgatcggg tacttttctt ccaaattttc tttgttcttt tcgatcaggc
     5461 ttttcgcttc ttcatatttt ttatctgcac atagtgcttt tacttcctgt aatacttttc
     5521 ctgcttccat ttgcctcacc ctcttgccat tttattgctt taagttttat ttaagttata
     5581 cgcattattc tagtttcatc ccaaataacc ttttttcatt tttactcctc gaactaatca
     5641 gttttctaaa ggctgattgg ttcgtctttt ttatttttcc tccatttcat tctgctcttc
     5701 tgttaattta tctaatgctt tgattgatgc tgcggctctt gcataatcat agctgtataa
     5761 ttccataaat ttatcaggca gaacaaacca cgctggatga taaccgttag ctgtgacagg
     5821 gaatccttta tcttgttggt tatctccatc ccatctcaga gcaacacctt tttttccttt
     5881 ccactttact aaagcgatcg agtatccctt ctttgtttgt gtgccgtctt caataattgc
     5941 taaaatttct attttttctt ttggcgcacg gacttcttga ggacgaacca ttttctattc
     6001 ctcccttatc tcattacttc acgaccaaca cactgcaagg ggcatgtgat acgacataag
     6061 aagcggtcga tccaactaat gcttgttgta tagcaccttt tcctgtcgct cccataacga
     6121 tcaaatcaat attttcttgt ccagggatga cattggcaat caaatttctt ggatctccag
     6181 tttcaactat tgcgtggatc tcagcaatgc cgtactcttt ggcatcattg atttttttca
     6241 gcatggctac ttctgttctt tgtttttcat ctgcatagat ttttgaaaag gcataggcac
     6301 tatgggtcag ctcaactttg ttgatgattg acacgataaa taagtgggct tcattgtcct
     6361 ttgctaagtc aagggcttca gcgaacgctt tgatcgattg tcttgatccg tcaactgcta
     6421 ccatgatgtt ctggtatctt ttactcattg gaaccgcctc ctttttcttt tattgtaaca
     6481 tctcaagcaa aaatacaaaa aatatacgca atatgtaaat aaaatgatga acaaaagcaa
     6541 tttctttcaa gttatttata catttctttt ctcatcagaa cacctgttct aatcccatta
     6601 tttctgcatt atgtgaaatt cctcatcatt tttcacatcg agcgaacatc attctgttac
     6661 aaaaaacgtg gatttgtcat aaattgtcat gcttctttta cctatgaaat attttcacgt
     6721 gttaaagtat tcctcgttga acacaacaaa taaaactttt taacgaataa aggagatttt
     6781 tcatgacatc acttaaaact ttactttttg gaacaacttt ggctgccggc gctgcattct
     6841 tcatgggaac gactgctcat gcggacgaag cttataccgt tcaatcaggt gatactctat
     6901 caacgatttc tcaaaaatac gttggtgata actccttgat caatgccatt gcagaatcaa
     6961 attc
//
LOCUS       DAYYFU010000096         6643 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000096 DAYYFU010000000
VERSION     DAYYFU010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.101"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACHA34_002600"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACHA34_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHA34_002601"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHA34_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFM8793186.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACHA34_002602"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACHA34_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACHA34_002603"
     CDS             complement(2143..2373)
                     /locus_tag="ACHA34_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFM8793187.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACHA34_002604"
     CDS             complement(2603..3421)
                     /locus_tag="ACHA34_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8793188.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACHA34_002605"
     CDS             3582..4271
                     /locus_tag="ACHA34_002605"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8793189.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACHA34_002606"
     CDS             4285..5787
                     /locus_tag="ACHA34_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8793190.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACHA34_002607"
     CDS             5800..6276
                     /locus_tag="ACHA34_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8793191.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACHA34_002608"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACHA34_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DAYYFU010000097         6600 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000097 DAYYFU010000000
VERSION     DAYYFU010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.102"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..486
                     /locus_tag="ACHA34_002609"
     CDS             <1..486
                     /locus_tag="ACHA34_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM8793192.1"
                     /translation="LIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLKDESNTTVSD
                     ANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKTGADIDHFDK
                     ALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQETIKKLRIG
                     G"
     gene            493..1344
                     /locus_tag="ACHA34_002610"
     CDS             493..1344
                     /locus_tag="ACHA34_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFM8793193.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSEPKNASGAYKRTGRREKLPEWAIDQEAYLKKKALERAN
                     RQSKAPF"
     gene            1356..1625
                     /locus_tag="ACHA34_002611"
     CDS             1356..1625
                     /locus_tag="ACHA34_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793194.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGINNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLSELEQV"
     gene            1630..1794
                     /locus_tag="ACHA34_002612"
     CDS             1630..1794
                     /locus_tag="ACHA34_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793195.1"
                     /translation="MDLKTFTAQIELMHQEALRQSASYEDKWLNTFHGGRESALDQVL
                     KLLKGERRDG"
     gene            1787..2089
                     /locus_tag="ACHA34_002613"
     CDS             1787..2089
                     /locus_tag="ACHA34_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793196.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVTEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEKK"
     gene            2086..2397
                     /locus_tag="ACHA34_002614"
     CDS             2086..2397
                     /locus_tag="ACHA34_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM8793197.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            2394..2612
                     /locus_tag="ACHA34_002615"
     CDS             2394..2612
                     /locus_tag="ACHA34_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HFM8793198.1"
                     /translation="MIGLTIFWCAIGGLLAFLGYYLICESRKGRKYTVTGIVLLIIGI
                     LVAVGAELNLYVNGTQEDLVRFMFWMKN"
     gene            2623..3438
                     /locus_tag="ACHA34_002616"
     CDS             2623..3438
                     /locus_tag="ACHA34_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFM8793199.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            3440..3565
                     /locus_tag="ACHA34_002617"
     CDS             3440..3565
                     /locus_tag="ACHA34_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793200.1"
                     /translation="MGFFMAKILLFLGLVGAAYLVYALLSQTDDKEDDNNDEMKF"
     gene            3577..3933
                     /locus_tag="ACHA34_002618"
     CDS             3577..3933
                     /locus_tag="ACHA34_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFM8793201.1"
                     /translation="MDLITQYSDIILKKIMAKIQKDKKAKERAELVKLEMAETGVGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKAHSIQHGFI"
     gene            3893..4138
                     /locus_tag="ACHA34_002619"
     CDS             3893..4138
                     /locus_tag="ACHA34_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793202.1"
                     /translation="MIKKLIQFSMDLYDIESGATVSVESDHLIISFADERQIIIWVVD
                     DMLYPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSK"
     gene            4135..4533
                     /locus_tag="ACHA34_002620"
     CDS             4135..4533
                     /locus_tag="ACHA34_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFM8793203.1"
                     /translation="MIPKFRAWDKRENTMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVEIFEGDVVITSKGAIGYVAYLQQEAGFVVVLKKSDYRLG
                     HRNTGESYDVATAHEVIGNIYENSELLEVE"
     gene            4530..4868
                     /locus_tag="ACHA34_002621"
     CDS             4530..4868
                     /locus_tag="ACHA34_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793204.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            4865..5095
                     /locus_tag="ACHA34_002622"
     CDS             4865..5095
                     /locus_tag="ACHA34_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793205.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            5102..5305
                     /locus_tag="ACHA34_002623"
     CDS             5102..5305
                     /locus_tag="ACHA34_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFM8793206.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASETIRKFGISILNDDRKWEAER"
     gene            5302..5607
                     /locus_tag="ACHA34_002624"
     CDS             5302..5607
                     /locus_tag="ACHA34_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793207.1"
                     /translation="MKLKDGFYTDAYGIGGLMMDLPTKNPIKQEESEIKVGDMVRCEA
                     EGFIYPFRGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVINNGIKN"
     gene            5591..5884
                     /locus_tag="ACHA34_002625"
     CDS             5591..5884
                     /locus_tag="ACHA34_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793208.1"
                     /translation="MELKIDKVTFEEPAFQEMDERIAELVEKKQDELIALLEKKYQFP
                     KYMNKMQAAKYLGVSYNTMMDKYVPAGLKMGIVNGVIRISKEECDRFMKEHQK"
     gene            6144..6560
                     /locus_tag="ACHA34_002626"
     CDS             6144..6560
                     /locus_tag="ACHA34_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8793209.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2470 a          969 c         1497 g         1664 t
ORIGIN      
        1 ctcataggcg atattttcat cgatacaggc atgccaacgg acttctggaa ggaattcttt
       61 tacttccgct ttgaaggtgt cacagggcgc aaaataagcc tcaaagatga atcgaatacg
      121 actgtgagtg atgctaacgt cttagcaaat atcatcttag atttcatctt tgaacatcat
      181 attcctttca aagaaggcta tgagatttta ccagcgaatc aagaatatta cttctacaaa
      241 tgcatcacaa aaagagtctg ttgcatctgt ggcaaaacag gagctgatat cgatcacttt
      301 gacaaagcgc taggaagacg aaagcgcaaa gaagttgatc attcagagta cacatttgca
      361 gcactctgca gaatccatca cacggagaaa cacaagatag gtgtgatcaa tttcaaaaat
      421 aaatatcaaa tcaaagggat caagttaaac caggaaacaa ttaagaaact tagaatagga
      481 ggataaaaaa tattggctga caacaaacga tactactatt taaaactaaa agagaacttt
      541 ttcgatagcg acgagatggt tcttttagaa agtatgccag atggatacat ttattctaat
      601 attcttctca aactttattt aagaagtctg aaacatgaag gcaaactaat gtttaatgac
      661 aggattccat tcaattctac aatgcttgcg actattacaa gacactctgt gggagttgta
      721 gaaaaagcag tacaaatatt tcgtgattta caacttattg aggtattaga taacggagca
      781 atttatatgt ctgatataca aagttttatt gggaaatctt caactgaagc tgataggaag
      841 agaaaataca gaaaagaaat tgaggaagca aaacagaatt taataacaag tggacaaatg
      901 tcggacaaat gtccggacaa aactacacca gagttagaga tagagttaga gaaagatata
      961 gagttagaga aagatataga gaatgtaacg ccttcgaaaa aatcgaaggc taagcccgtc
     1021 cgtcataaat acggagagta taaaaatgtt cttttgtcag atgagcagat ggagaaactc
     1081 aaaatagaat tccctaatga ctatcaagaa cgaatagaac ggctatctga gtattgtgaa
     1141 tcatctggta agacttataa aaactatttg gcaactattc gaagttgggc aaggaaagaa
     1201 aaaagtgagc ctaagaatgc aagtggtgca tacaagcgca caggaagacg agagaagctt
     1261 cctgaatggg caatcgacca agaagcctat ctcaagaaaa aagcgctaga acgagctaat
     1321 agacaatcaa aagcaccatt ttaagaggtg gaaaattgaa aatcgattat ctagaactaa
     1381 ttaatgaaat agcaaagtat aaaactggtg aggaaataga aatcctgaga gacgtatatg
     1441 atcaactcga agaagctgga atcgaaggaa ttaataatga tcgttcgagt tggagtaaac
     1501 tcagatacta tttcgcactc tatatcgata caacacaatt aagaaattta gcttatacaa
     1561 aattactatt tgttgattgc gttaaaggat tgcaaaaaca tcttagtgaa cttgagcagg
     1621 tgtaatcaga tggatctaaa aacatttaca gcacagatcg aactaatgca tcaagaagct
     1681 ttaagacaaa gcgcctcgta cgaagacaag tggctcaaca cgttccatgg tggacgtgag
     1741 agcgcacttg atcaagtact caaattattg aaaggggaac gtcgggatgg ataagaaagc
     1801 ggcaatgaaa cgaatcatcg aactgacaca ttctgagaat tggcaagaag acaaagaaat
     1861 agttacagaa gtccaaaagc tcggtaaatc aatgtggact gaaaagccca aacggagaac
     1921 gccgagaaag attgcaattt ggcatggtga tcgaattcta gtaacaggta ctgctgaaca
     1981 gttatctgaa attactggtc tgagcaaaaa catcatctgg gatagagcaa ggagcttatg
     2041 gattgattca aaaggacgac aatttaggta tgtggaggag aaaaaatgaa cgaactaatc
     2101 acaaaagtag agcagtgggc taaagacaag ggactggatc aagcagatcc aaaagcacag
     2161 tttttgaaag tagctgagga attcggggaa atcgcttcag caatggcaag aagtaatgat
     2221 gagctattta aagatagcgt aggagacgta atcgtcacgt tgattatcct ttccatgcaa
     2281 aaagggacaa acgtacaaga gtgtttagaa atggcataca acgaaatcaa agggcgcaca
     2341 ggaaaaatgg tagatggtgt attcgtgaag tcgagtgatt tggaggacag caaatgatag
     2401 gtttaacaat attttggtgt gcgattggtg gtttactagc atttctagga tactacctca
     2461 tctgtgaaag tagaaaagga agaaaatata cagtgacagg tattgtcctg ttaattatcg
     2521 gaattctagt tgcagttgga gcagaactga atctttatgt aaatggtaca caagaagatt
     2581 tggtgcgatt tatgttttgg atgaagaatt aggaggaaaa aaatgaacga aaacaaatta
     2641 atcaaattag gtgtagcagg agcagtaatc gtaggtattg gagttatcgg aggatttaag
     2701 ttcttcgaaa aaatcgataa tggatatgtg ggtgtgcgct attcaatgaa cggcggtatc
     2761 aaagatgaag cactgacgca aggtgtgaaa tttgtaggga ttgacaaagt gatccaatat
     2821 ccaattcgct tgcaaactat ccaatcaaaa aatatttcag tatctacaag cgacggcaaa
     2881 aagacaacga ttgatatcaa atatgactac aaagttgatt caactaaagc agcaaaaatg
     2941 tacaaggaat ttgggaatat cacttcggaa gatatcgaaa gtggatggtt aaaatctaag
     3001 cttcaaaagg tcgctcgtga agtttatgcg aaatatagtc tgcttgatgt cctttcagga
     3061 gattcctcta aagttgaagc tgaggtatta acgaactttg ctaaatcagt tgaatctaaa
     3121 gggtttgaag tcgaagacgt aacacttggt gttccagatg tcgataaaga aacacaaaaa
     3181 tcaatcgatg cgatcattcg agctggtcaa gaaaatgaaa aagcgaagct agatgcagaa
     3241 actgcaaaaa ctcaagctga tagtgaagct tacaagaaaa caaaagctgc agaagcagag
     3301 gcagaatcta atcgcaaagt cgccgaatca gtaacagaca atttgattcg ttatgaagaa
     3361 gctcaagctc gcaaaaagca tggatgggta acagtaaatg gagcagatac tgtagttacg
     3421 gatgaagcag gcaaataata tgggattctt tatggctaaa attctcttgt tcttaggttt
     3481 agtaggagca gcatatctcg tgtatgcgct cctttcccaa actgatgaca aagaagatga
     3541 caacaacgat gaaatgaaat tttaggagga gaaataatgg atctcattac acaatacagt
     3601 gatatcatcc tcaagaaaat catggcaaag attcagaaag ataaaaaagc aaaagaacga
     3661 gcggaattag ttaagttgga aatggctgaa acaggagtag gagtgcgaag tagcaggcat
     3721 tggaaagcag cagcaaacat tgaattttat tacaacgaaa ttcaaaaagg gttcgatcag
     3781 atgcgtgagc tggatcggca aacaaattgg agcaagaaac ttcatcaaga tcgtttcaaa
     3841 tttgtagaga agtataaaga aatattagaa gagtatttga ggaggacagc gaatgataaa
     3901 aaagctcatt caattcagca tggatttata tgatatcgaa tcaggagcaa cagtatctgt
     3961 ggagtctgat catttaatca taagttttgc tgacgaacgt caaattatca tatgggtagt
     4021 tgacgatatg ctatatccag aaattgtaca tgattttgaa gaatcaaaag cggttgagtt
     4081 tgaaatagtg aaaaaagtaa tggaattgat tgaaaaatac gaggaggaca gcaaatgata
     4141 ccgaagttta gagcgtggga taagcgagaa aacacaatga gagatgtagc tgtcttacat
     4201 tttactaaaa acggcaaaac aaactttatt gaatattgga taaatcctac cgaattgaaa
     4261 tcatatcacg tgcgaaacat cgacctcatg caatccacag gaatgaaaga taagaatggt
     4321 gtggagatat ttgaagggga tgtagtaatt acatcaaaag gagcgattgg atatgttgcg
     4381 tatcttcaac aagaagccgg ctttgtagtt gtactaaaga aaagtgatta tagattaggc
     4441 cataggaata ctggcgaaag ttatgatgta gcaactgctc atgaagtcat cggaaatata
     4501 tacgagaata gcgagttatt ggaggtagag tgattatctt agctactgac tacatcgatt
     4561 ctttaaaaga tgaaaatggg aatgtcccag cattgctaaa ttgtggaatt aaaagcaaga
     4621 atagaatcat tttgttacgg atatcggctg atttagcaaa atatctttat ctgagcaaga
     4681 cagcagtata ttacactgcg gctacacgtt atcagtacaa gggaaaaaca atcagtcagg
     4741 attattatga tcgtttaatc agtcctgaca tgcatggaaa atcaaaatca gcgatcaagt
     4801 tatttggcgc aatagagata tatgccgatg attttccaaa tttatggttg aaggaggaag
     4861 cggaatgaaa tacgaaatac cactaagtga agcgggcatt caagcaatta tcaatggtcg
     4921 ggaggttaat aaaaaacttc ctgatggtac tgaattagtc atcagacaaa gttatttgaa
     4981 agatatggca gctccagtat taattgatcg ttttaacgtg actgattctg tggtagagaa
     5041 ccacttgaaa gaatttcgat caagtataaa cgacactttc agattaggga gttgattgac
     5101 aatgaacacc agacaccgca gagtagcaaa actaagaaaa caggaactga atgtactaaa
     5161 gacaaagttt gaaaaagaat atggaatttc agcagaagaa acatataaag tggcaagtca
     5221 gtttgttgct gatgcgagtg agactattcg taagtttggg atttcgatat taaatgatga
     5281 tcgtaaatgg gaggcagaaa gatgaaacta aaagacggat tttacactga tgcttatgga
     5341 attggtggtt tgatgatgga tctaccaact aagaatccta taaagcaaga ggaatcagaa
     5401 attaaagtcg gtgacatggt tcgctgcgaa gcagaagggt tcatctatcc atttcgtgga
     5461 tatgtagagc atctctataa tcactcagcg atcattcgta ttgaaaacac aatggaatgc
     5521 gataagtggt tagcgaaaag caaagagaat ttagcagtgg tgagattggt ggatatggag
     5581 gttataaaca atggaattaa aaattgacaa ggtaacgttt gaagaaccag catttcaaga
     5641 aatggatgaa cgaattgcag aattggtcga gaaaaaacaa gatgaattga ttgctttgct
     5701 agaaaagaaa taccaatttc cgaaatatat gaacaaaatg caagcagcaa aatatcttgg
     5761 tgtaagttac aacacgatga tggataagta cgtaccagct ggtttgaaaa tgggtatcgt
     5821 taacggcgtt attcgtatca gcaaagaaga atgtgatcgt tttatgaaag aacatcaaaa
     5881 ataaaataaa aaaacgttta gccccttagc taaacgcaca attataattt acctgacagt
     5941 taattataac aaaattgcgg aggttgagga atggcggaat gaaaaagaca ataaaaacag
     6001 aagaagcata tattgaagta tttgacacac cagcatgcta agtcgagaag tgtaactcgg
     6061 caaataaaaa aagccggatc gctccgactg atgtaataaa cccaacaagt ttattatatc
     6121 acataaagga gcggtttgac ttgatgcaat tgttacgaga ggtagatttc aaacagacaa
     6181 gatgcaatgc gagagatgtg ctgaagaatt ttcgacgctt agagcggatg gcaggtcgct
     6241 ctttgataga tattaagtcg ccgattatta cggatatgcc gaaggcaccg aagcacggca
     6301 ataaggcaga ggacgcgatt attcagatga tggatataga agcagagaga gatgcgattc
     6361 tagcggcttt gatggctctt agtctgatta gccgtcagat actctattat agcttttgtg
     6421 acgtaaacaa gcactctaat tatgaaatag ggcaattgat acgaggatac ggagagaaga
     6481 atgtagagaa gctgaaatct atcgcgctga tcgaatttgc tgaagcatac aaaaaaggcg
     6541 tattagttca gtatcgttga ttttgtaggg tttttgtagg gatagtgtag ggtttttgag
//
LOCUS       DAYYFU010000098         6570 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000098 DAYYFU010000000
VERSION     DAYYFU010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.103"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACHA34_002627"
     CDS             127..579
                     /locus_tag="ACHA34_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793210.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACHA34_002628"
     CDS             593..1282
                     /locus_tag="ACHA34_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793211.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACHA34_002629"
     CDS             1407..3758
                     /locus_tag="ACHA34_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFM8793212.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="ACHA34_002630"
     CDS             3838..4233
                     /locus_tag="ACHA34_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793213.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="ACHA34_002631"
     CDS             4558..5175
                     /locus_tag="ACHA34_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8793214.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="ACHA34_002632"
     CDS             5216..5515
                     /locus_tag="ACHA34_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFM8793215.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="ACHA34_002633"
     CDS             5549..5716
                     /locus_tag="ACHA34_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8793216.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="ACHA34_002634"
     CDS             complement(5761..6366)
                     /locus_tag="ACHA34_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8793217.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2265 a          910 c         1285 g         2110 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaattttt
//
LOCUS       DAYYFU010000099         6516 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000099 DAYYFU010000000
VERSION     DAYYFU010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.104"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(207..1169)
                     /locus_tag="ACHA34_002635"
     CDS             complement(207..1169)
                     /locus_tag="ACHA34_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8793218.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="ACHA34_002636"
     CDS             complement(1171..2610)
                     /locus_tag="ACHA34_002636"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFM8793219.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="ACHA34_002637"
     CDS             2795..4741
                     /locus_tag="ACHA34_002637"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFM8793220.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /gene="scrK"
                     /locus_tag="ACHA34_002638"
     CDS             5005..5877
                     /gene="scrK"
                     /locus_tag="ACHA34_002638"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8793221.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            6173..>6516
                     /locus_tag="ACHA34_002639"
     CDS             6173..>6516
                     /locus_tag="ACHA34_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8793222.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALECFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTG"
BASE COUNT         2083 a         1136 c         1159 g         2138 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa acgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacacgcc gtttgtataa ttaagcgaga caaataaaaa agccatttat
     6121 gttacactca aatcgtaata cccgctaaag catacaagga gagtgaacat aaatgactta
     6181 tacccatctt acaccaaacg agcttgtaat gatagaagca tattttcatc aagaaactcc
     6241 ggttgctatc gttgcgaagc agcttaaacg tggacgccaa acaatttaca atgtctataa
     6301 ctttctcaaa tgtggtggaa cagcacttga atgctttgaa caatacaaag aaaataagcg
     6361 acgttgtggg agaaccgaaa tcatttttcc tgctgaggaa aaagaataca ttgcaaaaag
     6421 atcaactgaa ggttggaccc cagacgtcat tattggccgt gcagagcgaa ccttctcttg
     6481 ttcagtaagt accctctatc gccggtttaa gacggg
//
LOCUS       DAYYFU010000100         6303 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000100 DAYYFU010000000
VERSION     DAYYFU010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.105"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            334..714
                     /locus_tag="ACHA34_002640"
     CDS             334..714
                     /locus_tag="ACHA34_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM8793223.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="ACHA34_002641"
     CDS             696..1610
                     /locus_tag="ACHA34_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8793224.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="ACHA34_002642"
     CDS             1855..2544
                     /locus_tag="ACHA34_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793225.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="ACHA34_002643"
     CDS             complement(2665..2751)
                     /locus_tag="ACHA34_002643"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8793226.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="ACHA34_002644"
     CDS             3427..4377
                     /locus_tag="ACHA34_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM8793227.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="ACHA34_002645"
     CDS             4398..4931
                     /locus_tag="ACHA34_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8793228.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="ACHA34_002646"
     CDS             4947..5117
                     /locus_tag="ACHA34_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793229.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="ACHA34_002647"
     CDS             5567..6100
                     /locus_tag="ACHA34_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793230.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            6112..>6303
                     /locus_tag="ACHA34_002648"
     CDS             6112..>6303
                     /locus_tag="ACHA34_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793231.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKS"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DAYYFU010000101         6287 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000101 DAYYFU010000000
VERSION     DAYYFU010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.106"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..690
                     /locus_tag="ACHA34_002649"
     CDS             190..690
                     /locus_tag="ACHA34_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8793232.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="ACHA34_002650"
     CDS             787..2634
                     /locus_tag="ACHA34_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFM8793233.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="ACHA34_002651"
     CDS             2637..3533
                     /locus_tag="ACHA34_002651"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8793234.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="ACHA34_002652"
     CDS             3583..3972
                     /locus_tag="ACHA34_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8793235.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6287
                     /locus_tag="ACHA34_002653"
     CDS             3959..>6287
                     /locus_tag="ACHA34_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8793236.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacg
//
LOCUS       DAYYFU010000102         6173 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000102 DAYYFU010000000
VERSION     DAYYFU010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.107"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACHA34_002654"
     CDS             725..811
                     /locus_tag="ACHA34_002654"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8793237.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACHA34_002655"
     CDS             complement(932..1621)
                     /locus_tag="ACHA34_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793238.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="ACHA34_002656"
     CDS             complement(1628..1906)
                     /locus_tag="ACHA34_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793239.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="ACHA34_002657"
     CDS             complement(1866..2780)
                     /locus_tag="ACHA34_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8793240.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACHA34_002658"
     CDS             complement(2762..3142)
                     /locus_tag="ACHA34_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM8793241.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACHA34_002659"
     CDS             complement(3592..3879)
                     /locus_tag="ACHA34_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8793242.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACHA34_002660"
     CDS             complement(3899..4123)
                     /locus_tag="ACHA34_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8793243.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACHA34_002661"
     CDS             complement(4578..4748)
                     /locus_tag="ACHA34_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793244.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACHA34_002662"
     CDS             complement(4763..5296)
                     /locus_tag="ACHA34_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8793245.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACHA34_002663"
     CDS             complement(5317..>6173)
                     /locus_tag="ACHA34_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM8793246.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1633 a         1152 c         1042 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaacaaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAYYFU010000103         6149 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000103 DAYYFU010000000
VERSION     DAYYFU010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.108"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..2543
                     /locus_tag="ACHA34_002664"
     CDS             102..2543
                     /locus_tag="ACHA34_002664"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFM8793247.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2873..3577)
                     /locus_tag="ACHA34_002665"
     CDS             complement(2873..3577)
                     /locus_tag="ACHA34_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793248.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3764..4009)
                     /locus_tag="ACHA34_002666"
     CDS             complement(3764..4009)
                     /locus_tag="ACHA34_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793249.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4026..4877)
                     /locus_tag="ACHA34_002667"
     CDS             complement(4026..4877)
                     /locus_tag="ACHA34_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8793250.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5043..6047
                     /locus_tag="ACHA34_002668"
     CDS             5043..6047
                     /locus_tag="ACHA34_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFM8793251.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1971 a         1064 c         1302 g         1812 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttatttta tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta
     2641 gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca
     2701 ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc
     2761 tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat
     2821 gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat
     2881 cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt
     2941 atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg
     3001 ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt tcattttgtt
     3061 ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg
     3121 cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt tccgtttttt
     3181 cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt
     3241 catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga agattgtcac
     3301 ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca gtattgaagt
     3361 tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct aatccgatcg
     3421 ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca taacgtcgca
     3481 agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat gaaggacgga
     3541 attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt cgctcatacc
     3601 ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta
     3661 tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga aaaataaaaa
     3721 atggaacgga ttatgtatat atacataatc cgttccattc tttctatttt ttcggtatca
     3781 aggcaaaaac aggtagttca ggtgaataaa tgacttcatg ttcgtgccag ggtttgacca
     3841 ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat ttgatcatgt gtccagtttc
     3901 ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa gacaaaatcg gaatgaaaag
     3961 tctgggtaac ttcttccgag agagaattca agcgttcgat tggatccata aaatcacctt
     4021 tcgacttaaa attgggtagt atctgggtct tttgctttgc cgactacacc atctaactga
     4081 tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa ataattcagt attttctttg
     4141 attctgcttg gtgtcactga cttaggaaga ggaaggaatt cgtgttgcag cgaccaacgc
     4201 aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga tttgtttcat ttcaggcaca
     4261 tcgaagattt taccagtgcc taatggacta tatgcttcga ggatcatatc atgttttttt
     4321 gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa ggaaaacttg attgaccatc
     4381 ggcataattt ttgctgtttt tgctagtgta tctagatggt ggggtaagaa attgctcaca
     4441 ccgattgctt tgattttacc tgcttcatac agttcttcaa atgctcgcca tgtatctgca
     4501 tttgcttgtt cccaattatc acgaaaagca acagggtttg gccagtggat caaaaacaga
     4561 tctaaataat ctgtttggag cttcttcaat gattcttcaa aagaagacat cacaagttca
     4621 tagctgtggt ttgcattcca taattttgtt gtcaagaata tctcttctct aggaattcca
     4681 ctttccttaa tcgcttggcc gacgctttct tcattcttgt atccttgggc agtatcgata
     4741 tggcgataac ctgcagccag cgcttcttta acagaggaaa cagcgacatc cccatcaggt
     4801 gtttgccatg tgccgaatcc tactaccggg atttcgtaac catttgatag acgataagtg
     4861 cttgttaatg aattcatact ttcagctcct tttttattcg ttcaactata agtgtagcgc
     4921 atttttcgaa aaaaagaatc cattttgttt ctactcttga aatttttgtt taaaagaaaa
     4981 tgattttgat tctggtattt ttgatttttt ttccgtaaac taagacagaa gggaatgaga
     5041 aaatggatat ctatttaaaa aaagcaattc tgcatattat tgacagagaa actggttctc
     5101 cagtcttttc acaaaaagag ctggatctga caaaagaata tattcgtgat tttcttcaaa
     5161 agaagatcca aaaaatctct tctgctcaaa caaaaactgg acaactagca gaagaaagca
     5221 tgatcgttga atcacttaaa atcgctcaag aaaattttgt ggaagcaagt gaagcaatcg
     5281 ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc acctagtgca gatgtatttg
     5341 ttgtcttgta tgaattagat acagtgatgt acttatcttt attgaaagtc aattatcgtg
     5401 aggcttacac tcactttgtt gaagcagacg aagcagggat cgacaaccgc ttgattttga
     5461 atcgagcaat tctaggggcg aaaacacaaa aagcagatga agcattcgct gtgaatttga
     5521 ctgattggac ctacgaatta attgaaaaaa aatatgagtt ttctggcaaa aaagagcttt
     5581 atttttctgg aagagtgatc gaaagtgttc ctgcaccttc tttagaagaa aatgtaaaag
     5641 tgatccaaaa agtagccaag catcttggaa aaaaatttga gacagaagaa tttgatatca
     5701 tggctgattt gaaagaagct gtctatgata cgatagaaga aaaaggtcgg ttggatcatg
     5761 agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc aaaaatggct tttaaagaag
     5821 aagtacaaga gcaaggattt gtcccagaag cacctcctgt aaaagaagta caagaaattt
     5881 ctgagaaaaa atttggaaaa caaaaattca agctatcaaa cgggatcgaa ctgatcgtac
     5941 cagtagatgt atatcgcaac ccagatttga ttgaatttgt gaataatccg gatggtacga
     6001 tatccgtgat gatcaaaaat gtggatgaag tattgaatcg tttataaaaa tgactagaat
     6061 attaggtgtt caagtaccag tgatcagtta aatgaattgt gtcaagagaa ggatcggaaa
     6121 agacgaatat aacaaaaaaa gagcgtgag
//
LOCUS       DAYYFU010000104         5859 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000104 DAYYFU010000000
VERSION     DAYYFU010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.109"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..395
                     /locus_tag="ACHA34_002669"
     CDS             <1..395
                     /locus_tag="ACHA34_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM8793252.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="ACHA34_002670"
     CDS             398..1228
                     /locus_tag="ACHA34_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFM8793253.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="ACHA34_002671"
     CDS             1244..2095
                     /locus_tag="ACHA34_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8793254.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="ACHA34_002672"
     CDS             2092..2451
                     /locus_tag="ACHA34_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFM8793255.1"
                     /translation="MNKYRNKKTVHRGIKSDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="ACHA34_002673"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="ACHA34_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="ACHA34_002674"
     CDS             2624..2929
                     /locus_tag="ACHA34_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793256.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="ACHA34_002675"
     CDS             2929..3240
                     /locus_tag="ACHA34_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM8793257.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="ACHA34_002676"
     CDS             3237..3410
                     /locus_tag="ACHA34_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793258.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="ACHA34_002677"
     CDS             3407..3823
                     /locus_tag="ACHA34_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFM8793259.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="ACHA34_002678"
     CDS             3823..4491
                     /locus_tag="ACHA34_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFM8793260.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="ACHA34_002679"
     CDS             4488..4865
                     /locus_tag="ACHA34_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793261.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="ACHA34_002680"
     CDS             4862..5161
                     /locus_tag="ACHA34_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793262.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="ACHA34_002681"
     CDS             5151..5309
                     /locus_tag="ACHA34_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793263.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="ACHA34_002682"
     CDS             5403..5819
                     /locus_tag="ACHA34_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8793264.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2175 a          886 c         1361 g         1437 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtctg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgag
//
LOCUS       DAYYFU010000105         5809 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000105 DAYYFU010000000
VERSION     DAYYFU010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.110"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            504..1604
                     /locus_tag="ACHA34_002683"
     CDS             504..1604
                     /locus_tag="ACHA34_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain"
                     /protein_id="HFM8793265.1"
                     /translation="MIGIFSIGITNTYASENSNFNINSQPIEEQAINFAKTDFEHSIT
                     SILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDADNTIKYVYTVKKNTNG
                     EYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIKLLWKSPLSTEKNGVS
                     SFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMINWRIAETQTSFPWC
                     AAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQIIKYANYCGVYPK
                     KVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQGGYQTYYVWNPW
                     YNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1577..2020
                     /locus_tag="ACHA34_002684"
     CDS             1577..2020
                     /locus_tag="ACHA34_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793266.1"
                     /translation="MGQYHYKLVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="ACHA34_002685"
     CDS             3143..4075
                     /locus_tag="ACHA34_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8793267.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="ACHA34_002686"
     CDS             4092..4607
                     /locus_tag="ACHA34_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8793268.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="ACHA34_002687"
     CDS             4903..5184
                     /locus_tag="ACHA34_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793269.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="ACHA34_002688"
     CDS             5186..5602
                     /locus_tag="ACHA34_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793270.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DAYYFU010000106         5790 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000106 DAYYFU010000000
VERSION     DAYYFU010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.111"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACHA34_002689"
     CDS             complement(99..1229)
                     /locus_tag="ACHA34_002689"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFM8793271.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="ACHA34_002690"
     CDS             complement(1877..3079)
                     /locus_tag="ACHA34_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8793272.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="ACHA34_002691"
     CDS             complement(3139..3342)
                     /locus_tag="ACHA34_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8793273.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4548)
                     /locus_tag="ACHA34_002692"
     CDS             complement(3484..4548)
                     /locus_tag="ACHA34_002692"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8793274.1"
                     /translation="MDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERFE
                     ELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQF
                     FRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINNE
                     SNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAKI
                     ISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQSF
                     EKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYYQ
                     EEVEYYERKLQSISNEKTSY"
     gene            complement(4609..4716)
                     /locus_tag="ACHA34_002693"
     CDS             complement(4609..4716)
                     /locus_tag="ACHA34_002693"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8793275.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4879..5691
                     /locus_tag="ACHA34_002694"
     CDS             4879..5691
                     /locus_tag="ACHA34_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8793276.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DAYYFU010000107         5611 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000107 DAYYFU010000000
VERSION     DAYYFU010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.113"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..2008)
                     /locus_tag="ACHA34_002695"
     CDS             complement(59..2008)
                     /locus_tag="ACHA34_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8793277.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2050..3108)
                     /locus_tag="ACHA34_002696"
     CDS             complement(2050..3108)
                     /locus_tag="ACHA34_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFM8793278.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3227..3682)
                     /locus_tag="ACHA34_002697"
     CDS             complement(3227..3682)
                     /locus_tag="ACHA34_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793279.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4266..5069)
                     /locus_tag="ACHA34_002698"
     CDS             complement(4266..5069)
                     /locus_tag="ACHA34_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8793280.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1653 a         1108 c          777 g         2073 t
ORIGIN      
        1 cttacgaccc atcgttttat tctaaggttt tgtagtaaat atttcttgaa tttcatagct
       61 acgcccctcc aatcgaaaat acaatcttgt tttctttact ttcagcaacg gaaggctttg
      121 caataatttc agcattagaa tcaccttttc cttcaaacgc tccggcttca ctcaacttaa
      181 catcgaatat ttttgcgttt ggataagtaa ttattgcttc agtaatcgct tcttcaaact
      241 cagattcaac taaatgcaag atattccttg caccccctga tttagaatcc tcttgtgaag
      301 ccatgtcaaa aactaagtgc atggagagat cgtctgcata aaaattttga ccatatactc
      361 tttctttcct aggtggaaga cgtaaatcta tatttaaatg ttttcgtact aattcagcac
      421 tcttcaacat ttcattataa gcaatttcca gaagagtttg ttcggataaa gcccaaaatg
      481 gaactattcg tccaaatcgg ttaataactt caggctttag accttcacca atcaaggctt
      541 gtttgatccc tgatgcgact tttcggtatt ctctttttag ttcagtgctg tattcattag
      601 aattttcctt gtttgtaaca cgagatagtg agctatatcg gtttaacgaa tcgaacatct
      661 ccgcacctgc atttgttgtc gcaaataaaa ttgtatttga caatgaaacg gtgtcgtgaa
      721 cttctcgatc atcccatcct ctttcagtaa aacttatcct accttcatct aaaattgata
      781 aaaaggcatc gagtgtttct acaccagctt tctcatattc atcacacaca ataatcgcag
      841 aaggatcatg ccttacatga gtacctataa gtcgtctaaa acgcattgca tcatctttgg
      901 ttttgtagtc cggcatatta actaagagaa ctttattttc atcgccataa agtcctcttg
      961 ccacggcttt agccattgca gttttaccga cccctgttga tcctgtaaac agagcagttg
     1021 cttttggttg ctttcgacct ttaaaacgat caaatccaag cttcatacgt ttaaacaaca
     1081 ttgttatagt ataaactgct aacggttgtc cttttatgga ctttaattcc tcttgaagtt
     1141 tttccatatc aaaatcagaa tctagctgaa tattatactt accaaatatt tgcaatagac
     1201 tttctcgatt ataagcattt ccgtctactc gatcttttgc aatcaaatcc tccattacag
     1261 caattgattt agccggttcg gacaaatcct cacgataaag tttgtttgct tgtacgattt
     1321 cttgaagtaa ttcatcagga atcctttgcg ataatctaaa agaagcctga ggatcatctt
     1381 taacagagga tgtatttagc cattgtctca aaatatcgac gacaacatcg ctttttagtt
     1441 cattcaattc taacggttgt agccgtctcc ctaaggcatc atctcttttg atatagtttt
     1501 catattcttt tcttgtagta gcggtaatca tttgaagtga ttcatttgct aacggttctt
     1561 ttagcgaatc acccccaatt ttattgccac tcccaaaaga gctaacaact ttatgaaact
     1621 cgtcaatgaa gaggacaaat tcattatctt tgtcctcagc ttttaaaatt cgctcatatt
     1681 ctttcaatct tggaattaat gtttctaaac gtgtttggag gacatcatct cccccaacac
     1741 gcaattgtcc gattgataat ttatatactt gtactttctt tccatcttgt cgctgtcttt
     1801 cagcccattc acttgcaagt gctgttttac ctgttccggc ttcacctaac aagatagctt
     1861 gcggtgtttc cggtctattc aagatcagtg acaactcatg gagttcgtca tcacgtccca
     1921 ctaacttatg ttttggtggt ttcattggtg tgagagcatg attaaattcg gtttgaatat
     1981 tcttaaaaaa ctcggtacta ttttccatct gtttgtgttc acttcctcta aaataagaat
     2041 aatttacttc tagtcagtca aagttaggta cttaaatgtc gtcttcatgt ctccaaaaga
     2101 atctttgtta aagtcagcat ttttgtattg atcaatcaat ttttgtgtat cattgattgc
     2161 tttaatttcg gtctccattc gactgtctag ttgacgaata ctttcttgcg aatctttatc
     2221 gtcaggattt tgttgtaaac gttccagata ctcagctttt gtggcttcaa ttttcttgat
     2281 gttagtattt gaagcattta actgatcttc tagcttattt acaacattat tcttacttgt
     2341 aactgctaca acttttgaat aatcaatatc gccatctttg gtaagaaagc ttccttgata
     2401 gacattagtt gaatctgaat atggatttat tctgaaattg atattatcag gagtattaac
     2461 aggttttgct tttttcccaa aagaaattac tccttcatta ttaacatcat tcatagaata
     2521 ttgagaaagt gcttttgtaa ctgaatctgc ttcattcaca ttgactttgg tatcatcgtc
     2581 agctgtatca tcaccagaaa ttagtgatac ttggtttgca ataaaaactt gataagctct
     2641 ttcctctaat ttacctttta ggaataagaa agcatcccca tcagtactta acatcccata
     2701 gctcatttca agcttcttag gacgatcttt ttcttttgtt gccatatata agttatagtt
     2761 ttttccttgc gtagataaca actttctagc gttttgggag taaccaagtt ttactactgt
     2821 tacatctcta ttttgatctg tccacacatc tgaaatagtg atttctgctc ctgatcgagc
     2881 aaactccaat gaagacccta taggtgttgt attgttcacg agataataat tatagtttcc
     2941 taattgataa atgtagctac ccaagcctgt taataaaaaa agcactccta gaaaagttgc
     3001 aagcataatg cgtaatccgt gacgtgttct cttatcctta tcaccaagca accctccaat
     3061 aatactgtat tgatctttgg tagatgttga attgacgttt ttttccatca gtttgtctgc
     3121 cccctttcat aaaagagcaa acaatcagac gttttttcgg taattctagg ttcgggattt
     3181 tgaatcattt cggtatattc ttccccatta atatttaaca caaaaattat cttcctttct
     3241 tcgttccttt cttttgctct ttattttcca aataatcaat gatttttgct attgtaccaa
     3301 gatagtaatt cccctcagct ttgttttttc ggattttgct aaccattgga gtaggtacaa
     3361 tctcttcatc tctcaattca ctgaaagatt ttttacctag atattttcca ataacagatt
     3421 ttaaaaaacg ttcaaaatct ttctcgtaag ttgttttctt gccttttagt tcatggttca
     3481 tattgtcaac gacttttgcg gtttcaataa ttgttttagt tctcagctta ataatttcat
     3541 ctggaatttt caatcctcgt aatttcatcg ttgtactaac tggtaaccca tgctttactg
     3601 catcagcaat actaaaactt tttagatcat ctagtaagct ctccatatag ttaacaaatt
     3661 catgccaatt aatagcattc attgtctcac cccttcattt ttttaaaatg aggttcgggc
     3721 gtaacacatt tatgttatta taatatcata ataacataaa taaatcaatc agcaaattta
     3781 gatatccctt aaagtatcag caattattca acaagatatt tattttagct atttcagtca
     3841 tcataaaaga ctaattagca ataaaataac taatattatc catttatcag acgaaaagaa
     3901 aaaacttatc taattactct atattaactt ttaacaaata gttgacttgt ccaaaaatgt
     3961 taccttcaat ctcacaaaga tattaagatg tttcctacca aaactgatat caccggactt
     4021 tttattctgt gttgtccaaa agtgttacct ttagtcgatc aacaaattaa aaaaaattgt
     4081 ttttttcttg acttgtccaa aagtgttacc catagttgtt aaaatttgta tagattagcc
     4141 attctttcga atattattta gactgaaact tgcttacaaa aaaacttctt gcatttattg
     4201 tccaaaagtg ttacccatac ttttttaaag aagcaggttg tattaaaatt acaacctgct
     4261 tcttctcaat cttcttctaa ccaattatgc attgaaatgg ttggcaagtt tgattctggc
     4321 tcactaaaag tgaatctgag ccgatcaact cttcttcctt tctttgcata gattttttca
     4381 taatcaaaag tttcaaatac ctgtattccg ttttcatcag gagctaaaaa ttcttctttc
     4441 gcctgctcta gtattctttt gtcaatattt gtcattctat aacttgaagg tatacttaat
     4501 gctcttctaa aatcttcaac tttgactacc caataaccat ttcgccactc tttatttctg
     4561 tattgcatga gatgacgata caattctttt gtgtagctac ttcttagttt tgtaaaatta
     4621 tcaagttcaa aacgagtaaa gtttttgcca attgagttta agataaattc aaattcctca
     4681 tttgccttaa ttgtaactgt ttccttctgt ttatctatcc ggaatttttg aaaaagccac
     4741 agctgttcat agacgtctga atcttcaaaa cggtaggtta aagtacctag tttgtcactc
     4801 aaagctttta acacagaata aaaactgtcc ttttctcttc tgtcgtattt gaccaaataa
     4861 cgaaagaggt tgaaatcaaa agttacttct gctgttcctt gacgtttcat ttttgataaa
     4921 atagcccaaa acacatccat ttcaacaggc ttaaatcctc gcaaagctgt tgtattcaga
     4981 ccattgttat aacgcacaat tgcatttgtt tgttctgata ctaaatctac atcactatca
     5041 ataatctttt cggtaacttc ttgagacatt gattactctc caatcttctt tttgcgctcc
     5101 tttaatcatg ttaatctggt actaattaaa ggaaacaatt ttttctgctt gttacctaaa
     5161 ttaaatcaca actaaaggta acagtcaaca aagatttgtt actaatctaa ttttctattt
     5221 tttcgccttc caaatcaaga aaaccgaagg taacgtctta tcctagtgtg atacctcaaa
     5281 tgtccacttt tgttaccttt gctgtccaaa agtgttaccc ttactgtcca cttttgttac
     5341 ctttactgtc caaaagtgtt acctttatac ccctttaata cttactctcc caagagatta
     5401 agcctcccct aacagatata ttaacagata tttaacagat atattaacag atatttaata
     5461 aaggaggatt actcctctct tatttcaaaa tcttaaaaat aaaaaatctt aaaaaaaact
     5521 aacactatct aggagtttat tttgcttgtg taactttgaa taattgaaaa aaaggaaaag
     5581 gaggaagctt cagcttcccc ttttatttat a
//
LOCUS       DAYYFU010000108         5532 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000108 DAYYFU010000000
VERSION     DAYYFU010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.114"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..985)
                     /locus_tag="ACHA34_002699"
     CDS             complement(<1..985)
                     /locus_tag="ACHA34_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFM8793281.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR-A"
                     /locus_tag="ACHA34_002700"
     CDS             1137..1832
                     /gene="vanR-A"
                     /locus_tag="ACHA34_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFM8793282.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..2964
                     /locus_tag="ACHA34_002701"
     CDS             1810..2964
                     /locus_tag="ACHA34_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFM8793283.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3179..4147
                     /locus_tag="ACHA34_002702"
     CDS             3179..4147
                     /locus_tag="ACHA34_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFM8793284.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4140..5171
                     /gene="vanA"
                     /locus_tag="ACHA34_002703"
     CDS             4140..5171
                     /gene="vanA"
                     /locus_tag="ACHA34_002703"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFM8793285.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5177..>5532
                     /locus_tag="ACHA34_002704"
     CDS             5177..>5532
                     /locus_tag="ACHA34_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFM8793286.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRG"
BASE COUNT         1669 a         1078 c         1252 g         1533 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat
     1981 gacttaaatc acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat
     2041 atctttattt atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca
     2101 aaattcgcaa aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa
     2161 gataaacaaa ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta
     2221 aaacggactc tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac
     2281 gttgttatgt acttggcgca cgatattaaa acgcccctta catccattat cggttatttg
     2341 agcctgcttg acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc
     2401 acgttggaca aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg
     2461 tataacctac aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg
     2521 cagatgaccg atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac
     2581 gcccccgagg atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac
     2641 attttgaaaa acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc
     2701 ctctccgggg atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag
     2761 ctagctgcca tatttgaaaa gttctatagg ctggacaatg ctcgttcttc cgatacgggt
     2821 ggcgcgggac ttggattggc gattgcaaaa gaaattattg ttcagcatgg agggcagatt
     2881 tacgcggaaa gcaatgataa ctatacgacg tttagggtag agcttccagc gatgccagac
     2941 ttggttgata aaaggaggtc ctaagagatg tatataattt tttaggaaaa tctcaaggtt
     3001 atctttactt tttcttagga aattaacaat ttaatattaa gaaacggctc gttcttacac
     3061 ggtagactta ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga
     3121 ttaccattgg catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat
     3181 gaataacatc ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc
     3241 tctttcgcct cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa
     3301 cgccaaatcc gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc
     3361 ctctattctt cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg
     3421 ctgcaatcat atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc
     3481 gtactcgccg gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa
     3541 cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg
     3601 caaggtactc agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt
     3661 tattgagcgg ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat
     3721 agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca
     3781 tgtgccgctc aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa
     3841 gcaaggagca tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt
     3901 taaagcatta gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga
     3961 agagtttttc tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact
     4021 tcaaagaatg cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt
     4081 gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca
     4141 tgaatagaat aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg
     4201 taaaatctgc aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca
     4261 ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa
     4321 acgacaattg ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta
     4381 aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca
     4441 agtcaggtga agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag
     4501 gctgcgatat tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga
     4561 aaaatgctgg gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg
     4621 cagctacgtt tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg
     4681 tgaaaaaagt caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg
     4741 acagcaaaat cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg
     4801 gaaacagtgc cgcgttagtt gttggcgagg tggaccaaat caggctgcag tacggaatct
     4861 ttcgtattca tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc
     4921 ccgcagacct ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata
     4981 aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca
     5041 ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta
     5101 tgatggccgc tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt
     5161 taaaggggtg ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt
     5221 tcgttgggac gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta
     5281 tgaagtaaat cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga
     5341 actggctgct acccaagggt acggattgct tctatgggac ggttaccgtc ctaagcgtgc
     5401 tgtaaactgt tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta
     5461 ttatcccaat attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag
     5521 ccatagccgc gg
//
LOCUS       DAYYFU010000109         5474 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000109 DAYYFU010000000
VERSION     DAYYFU010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.115"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..291)
                     /locus_tag="ACHA34_002705"
     CDS             complement(<1..291)
                     /locus_tag="ACHA34_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793287.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYI"
     gene            complement(589..1563)
                     /gene="sgrA"
                     /locus_tag="ACHA34_002706"
     CDS             complement(589..1563)
                     /gene="sgrA"
                     /locus_tag="ACHA34_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFM8793288.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1759..2598)
                     /locus_tag="ACHA34_002707"
     CDS             complement(1759..2598)
                     /locus_tag="ACHA34_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8793289.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2783..3670
                     /locus_tag="ACHA34_002708"
     CDS             2783..3670
                     /locus_tag="ACHA34_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFM8793290.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(4254..4949)
                     /locus_tag="ACHA34_002709"
     CDS             complement(4254..4949)
                     /locus_tag="ACHA34_002709"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFM8793291.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(4933..5331)
                     /locus_tag="ACHA34_002710"
     CDS             complement(4933..5331)
                     /locus_tag="ACHA34_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8793292.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1744 a         1098 c          802 g         1830 t
ORIGIN      
        1 aatataacca atcgttccct ctaaaagtat agaataaact ttactcagta aaaactgaga
       61 agaaaactcg acttttatca tcttgttttc tgaaagaaaa gataatttat attctggata
      121 cctttcgcaa aacgattctg cttgttgaat caccgaattc atcttagcca gggtgtagtt
      181 tagatctaaa gcgatatcct tcaccacata taatttctcg tctctttcat aacaatagtt
      241 caaaaattga tatacttgcc tttcattgat atcaataaat tcttcaaaca aagtacatac
      301 tcctttttat ctattattta gacttattcc gacagctaca tgaataacgt acaaatagat
      361 tcgttactga tttttcagag atactaccat gcgcagtgtg cattgtcaat tattcttgct
      421 cttattttcc atttgtgttc ctgttatttc ctgtttctca aatatgttag taaacaaaaa
      481 tagatctggc aaagctacaa cttcgccagg caatacagca ctctcgaatg aaatattcac
      541 tcaaaaatct atcgttttat tcaattttag atctgttgct tttattcttt atttcactcg
      601 atggaagaga gaacgaaaat agcttgctcc tacaatcaaa atgagtaaca cacccatcca
      661 tgtaattagc ttgttcaagg ttctactacc agtttctggt aaatcgcttt ttctattatc
      721 tgtttctttc gctttttctt gatgatcaat tccacgagaa acaaaatcag gctgttttag
      781 cacacttttt aggttgctcc ctgcatttct cttttgttcc ttagttggta tgataggtac
      841 caaatcaagt tcattattta cttttcctgt gctgctttcg ctgctcgtgc ttcctgtgct
      901 actctcacta ctcatgcttc ctgtgctact ttcactactc gtactctccg tactgctttc
      961 gctgctcgtg cttcctgtgc tactttcact actcgtactc tccgtactgc tttcgctgct
     1021 cgtgttttct gtgctacttt cactgctcgt actctctgta ctgctttcac cgctcgtgtt
     1081 ttctgtactg ctttcactac ttgtgctatc cgtactgctt ggcggtccaa caactgtcca
     1141 actgcttggt ttcaaattct ccgtgatcgt catctgtcca tcataaaaaa caatcaaatc
     1201 aactttatct ttactcttga tctcatcaat cgatagttct gcagaaatca ttattgtata
     1261 ctcaatacca gcggtccatt gtacgtcttt taaataaact tccccaataa ctttttctga
     1321 acttccatta ccatcccaac catatgttag aacagcatgt acatcactag cattcacgct
     1381 tttgtttggt ttgactggaa tagtaatgac ttgttgtgtc atgtcataaa caatccgatc
     1441 agttttcaca gtaaatgaaa gattctcatt tgcccgttca tttgcagaag tactgattcc
     1501 aatcattgct gcaattatac tcagcccaag aataacaaat aatacggtcg ctgttttctt
     1561 catttctatc actctccata taattacctt ctatttgata aaggaattat ataaaatgaa
     1621 aatgatgaga acacgacagt cagttgtgaa aatctaacaa aagatgtcgt gtattcgctt
     1681 gaatttgttt actttttcgt ttaatcaatg acagacttaa aaaagcaaga ccgatatcag
     1741 ccttgctttc ttcatctttt attgataaac agcagtgtat ttcttcatcc gctttgtttt
     1801 atatgcatag agtaacaaac caacaatcaa gactaacaag aataatgtag ttgatataga
     1861 tgcttcagcc ccaaagtttc ccccagtaat tattgtattt tgcgattgga tcagagtgga
     1921 gaaaaaacct tgttcttgcg ttccgctgac tggtataccc aaaatagctc cttgtgtcca
     1981 attccaagta atatgagcac caatagccgt aaataaactt cctgtactgt agataagtgc
     2041 gctgaaaaag caacctgcta aaaagatatt gaaaactgcc agaaatgtca tgttaggatt
     2101 caatccgtgc aaaagagcaa ataatgcagc agatccaatc attgctatta acggaccaaa
     2161 tctaagcatc tgatgctgaa aaaagccacg gaataaaagc tcttctgatg cccccactgc
     2221 aataaataaa ataaaactcg ttatcagcgg cacaatgttt gcgtttcctg aatctaaccg
     2281 atacgccccc gtcaaatata atggactagt aataagcaga aacactccaa ttccaacaac
     2341 gagacctccc gctaaatacc cccagtcttt tcctcgaaca gaaacaccat aatcttctat
     2401 tctttttcct tcgaccttac tagcaagaaa taaagctagt aaaaaaacag ctaaatgtcc
     2461 gaccgtaccg gcaattgctc caatccattc actatttcca ataaacatat ccgcaagtgc
     2521 attattgaca agaattccta aaacaaaacc aactaagact attaaaacag atccaatcaa
     2581 ccgaataatc ctgatcatct ttcatccctc ctttatatta agtttaacaa cccttttctt
     2641 tcttgtctcg tgaaaactat gtaaatgttc tccatcatag agagaataga tttttacgaa
     2701 ctaagaatat ctacagcaac cttacgaaaa tgaaagactg tctccctaat tcttctgtta
     2761 gactgttttt aataaggaaa ggatgggaaa ttttttgaaa aaacaactac taattatgtg
     2821 cctttcagca tgtgcaattt tactattctc aggttgtagc aagtccaatg aaacgattgc
     2881 aacgtttaaa ggtggaacac ttacagttca ggatttttat gataacccaa aagttaaatc
     2941 aaataatcaa acactattaa aaaaaatgat tgtcttcaaa gcatttgatg atgtatacgg
     3001 aaaagagatt tcttcaaagg aagtaacaaa agaatataat gaacaaatca aaaaactcgg
     3061 tccaaactac aaggaacaat taaaagcagt tggccaaaca gaagagacat ataagaaact
     3121 ctttaaacag atgctggctt tccaatacgg tttgaaagcc aatgtaaaat taacagataa
     3181 agatctagac actgcttgga aagaattcta tccagaagtt agtacacaga tcattctatt
     3241 ttcgactaaa gaagaagcag ataaagcaaa aaaagaagca aatgaagggg aaaatttctc
     3301 caagcttgtt caagcttatg gaaaaaataa aacattaaag gaaactgatg gaaaaatgaa
     3361 ttttgattca accaatccag agattcctac tgaagtaaaa aaagcagcat ttaaactaaa
     3421 aaacggggaa gtttctgata tcattcctgt aacagatcca acgacttatc agcaaagcta
     3481 ttatctagta aaaatggtaa aaaaacagga taaaggttct aataaagaca aatacaaatc
     3541 tgagctagaa aaaattgcta ccgaagcaaa aactcttgat acggaattca tggataaaac
     3601 aatccgtaaa gtgatgaaaa aagacaatgt aactattaaa gatccttatg taaaaaatat
     3661 attcaaataa acaaatttga cccaattggg gatgtggcaa aatttgagaa gtgatttttg
     3721 gaaattcctt cttatttttg aggcaaacca cttttgccac aacttcatcg gggccttttt
     3781 ttatttgtac caaaacaaat cactggattt gttacaatca tgaggatata atttaaaaaa
     3841 gttttttatt tctatcacat gaatcaatgc gccattaagt agattctttc atgcttccct
     3901 attttttcac ttgccgtatc gcttcaaaaa caactatcat aaactcaaac tcgactgaaa
     3961 agttatttta aaaaagacta aaaatatccc ttctggactt gttcctctta ttctgttata
     4021 ctagagaaac acgaaagata tttttatgcg acacttttca tttgttttta tgaaatttat
     4081 tcaaaactgg cgtaacttca gagttacaag gatgtattag aggttaggga atacatcgtt
     4141 taaatgagaa ataagtaata gtcacccaaa ctttaaatta atgaatattg aatcgtcaga
     4201 tagcctcctc gagttttcga gaaggctatc gtttatttta tggagaaatt gattcagaag
     4261 taatcagcga ccttttttag ttcaccaatc gtttctgctt ggaaagtttg cacccaatct
     4321 tcttggaagc ccctcactgc tttatcaatc gagggcatct ctaatacttg atctagtagc
     4381 tctttaccta acgttatcgc atgagctcca gatcgaatgg ctgtagtgac ttgttcacaa
     4441 tttttgaaac tagctgctaa tatttttgtt ggtctttgcg tacgttcgat ctcagtccgc
     4501 aacgaatcaa tcactttcat agcatcaatg ccttgatttt gcatcctgtt ataataaggt
     4561 gcaatataat ctgctccatt attaatagct aaataccctt gtaattctgt gtaaatagct
     4621 gtaccggtaa tcaaataacc ttcttttttc aactttttga tcgcttgaag gcctgcttga
     4681 ttcaccggca ccttgataaa cgtatctttt cccagttctt ctatgataac tcgagcatcc
     4741 tctatcattt cttcttccgt tcttcctatt gtctgaatat ggataggaat ctgtccgatt
     4801 atttttttga tttctttcat atgcgagaag aagtttgacg ctccttcttt tttcagaatt
     4861 gtaggatttg ttgttacgcc tgttaaattc aaaaactgtt gacaagaata aatacttttt
     4921 gtatttgctg tatcaagcat gatttccata tagctttccc ccattctgat cctcttgatc
     4981 gattttaatg attgtcccct ttcttaaatg cggcatttcc ttatcttcta catacattgt
     5041 tcctggaagt tccggctcat gagatccatc aaattttatc gtgatatgtc ctaattcatt
     5101 caaattagtt tggacaacat ctcctactgc tgtgattaca tacatttgtt tatcaaaaca
     5161 gagcttcata tcacaagtta tttcttttct tacctgattc aattcaatct tataacaata
     5221 atctgttaat gtttccggtg cttcatctcc aaataaaatc attatttttt ctttcctgaa
     5281 tacttctgct tctataccaa catctgttac aaccgtttca aagatcgtca tctctgttcc
     5341 tcctcgtaaa atatcatttg tctaatagtt tttattcaaa atgtttctag attataaaat
     5401 ttattgaata acacattcct aacggaattt gtttcgatat tgtgtgattt ctcagagtac
     5461 aatggatgta tgga
//
LOCUS       DAYYFU010000110         5443 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000110 DAYYFU010000000
VERSION     DAYYFU010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.116"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            74..1585
                     /gene="gadC"
                     /locus_tag="ACHA34_002711"
     CDS             74..1585
                     /gene="gadC"
                     /locus_tag="ACHA34_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HFM8793293.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            1598..2998
                     /locus_tag="ACHA34_002712"
     CDS             1598..2998
                     /locus_tag="ACHA34_002712"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFM8793294.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(3163..4603)
                     /locus_tag="ACHA34_002713"
                     /pseudo
     CDS             complement(3163..4603)
                     /locus_tag="ACHA34_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            4996..5443
                     /locus_tag="ACHA34_002714"
                     /pseudo
     CDS             4996..5443
                     /locus_tag="ACHA34_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
BASE COUNT         1735 a          882 c         1034 g         1792 t
ORIGIN      
        1 tgcaagctca aataagcgat tttccataac cttggtagga atgacaaatt aaataggaaa
       61 gggaagtaat aatatgaata aaaaaaagtt atctctattc ggtttttttg ccctgacagc
      121 ttccatggtt ttgactgttt atgagtatcc gacctttgca acatcacaat tacatttggt
      181 tttctttctt cttttgggag gtattttatg gtttctacca gttgctttat gtgctgcaga
      241 aatggcaaca gttgaaggat ggaaagatgg tgggattttc agttgggtta gtcaaacttt
      301 aggagaacgt tttggttttg cagctatttt tttccaatgg tttcaaatta cagtaggttt
      361 tattacaatg atctacttca tactaggagc gctgtcgtat gtaattaatt ttccagagct
      421 taatacagat ccatttatca agtttattgg tgtattagtc attttctggg tactgacttt
      481 ttcacagttg ggcgggacca atcgtaccgc taaaattgct aaagcaggat ttgtcattgg
      541 aattgttatt ccttcgatca ttctctttgg tttagcagca gcctacttta tcggcggtaa
      601 tccgattcaa atggctatta atgcgaaagc ttttattcct gacttttctc agctttcaac
      661 tttagtagta tttgtatctt ttatattggc ttatatgggc gtagaagctt cagcatcaca
      721 tattaatgag ctggaaaatc cccaaagaaa ctacccattg gcaatgtttt tactagtgat
      781 tttggccatc gccttagatg caatcggcgg tttctcagta gcagcggtta ttccacaaca
      841 tgacttgtct ctcagtgctg gtgtaatcca aacatttgaa acgcttattt tacattttaa
      901 cagtcattta cattggttgg taaaagtgat tgctctcatg attgcctgtg gcgttatggg
      961 tgaagttagt tcttgggtag ttggtccatc acgagggatg tttgcagctg ctcagcgagg
     1021 tctgttacca gttttcttcc ggaaaactaa taaacaagac gttccagttc cgttgattat
     1081 gatacaagga attatcgtta ctatttgggc agcagtctta acttttggtg gaggaggcaa
     1141 caatttatca ttcttagtag caatctcctt aactgttgtc atttatctcg taggatattt
     1201 actatttttc ctaggttact ttgtattagt ttttaagaaa aaagacttaa aacgaactta
     1261 caatgtacca ggaaaaactg ttggcaaaac gattatcggt ggaattggtt ttattttatc
     1321 agtgttcgca ttgcttatct cttttgtacc accagcttca attgcagcta gtgagcaccg
     1381 aacataccaa atcatactac ttattagctt tgtagtgact gttcttttac cttttatcat
     1441 ttatgaagta catgataaaa gtgctcacac aactattgag gaacctactc acgtgaaagc
     1501 tgaagacgtt aatcccgctg tttatccagc tgctagagga gagcaccacg ttacaaaaaa
     1561 tgatgaacat attttaaaac attaggagga attcattatg ttatacggaa aagataatca
     1621 agaagaaaaa aactatttgg aaccaatttt tggctctgca agtgaggatg ttgacttgcc
     1681 taaatataag ttaaacaaag aatccattga accacgaatt gcttatcaat tagtacaaga
     1741 cgagatgttg gatgaaggaa atgcgcgatt aaacttagct actttttgtc aaacgtatat
     1801 ggaacctgaa gcagtgaaat tgatgaccca aacgttagaa aaaaatgcaa ttgataaatc
     1861 agaataccca cgaacaacgg aaattgaaaa ccgctgtgta aatatgattg ctgatttatg
     1921 gcatgctcca aataatgaaa aattcatggg aacttcaacg atcggctctt cagaagcctg
     1981 catgctgggt ggtatggcca tgaaatttgc ttggcgtaaa cgtgctgaaa aattaggtct
     2041 tgatattcaa gcaaaaaaac ctaacctggt gatctcttct ggttaccaag tttgttggga
     2101 aaaattctgt gtatattggg atgtggaact gagagaagtc ccaatggatg aaaaacatat
     2161 gtcaattaat ctagatactg tcatggatta tgtggatgag tacacaattg gtattgtagg
     2221 tattatgggt attacttaca ctggtcgtta tgatgatatc aagggtctga atgatttagt
     2281 tgaagctcac aataaacaaa ctgactataa agtatacatt catgttgacg ctgcatcggg
     2341 tggcttttat gcaccattta ctgaacctga tctagtttgg gattttcaat tgaaaaatgt
     2401 tatctcaatt aattcttcag gtcacaaata tggtttggta tatccaggtg tgggttgggt
     2461 cttatggcgt gaccaacaat acttaccaga agaattagta tttaaagtta gttacttagg
     2521 tggagaaatg ccaactatgg ctatcaactt ctctcatagt gcagcacaac taattgggca
     2581 atactacaac tttgtacgct atggctttga tggttatcgt gatattcacc aaagaactca
     2641 tgatgttgct gtttatttag ccaaagagat cgaaaaaact ggtatttttg aaatcattaa
     2701 tgatggatca gaattgccag ttgtgtgcta taagctgaaa gaagatccca atcgcgaatg
     2761 gacactatat gatttatctg atcgtctgtt aatgaaggga tggcaagtcc cagcctaccc
     2821 attacctaaa gacttggatc aattaattat tcaacgctta gttgttcgag cagactttgg
     2881 aatgaacatg gctggtgatt atgtacaaga tatgaaccaa gcaattgaag agttgaataa
     2941 agctcatatt gtttatcata aaaaacagga tgtgaaaacg tacggcttta ctcactaaaa
     3001 ttcttcagag aaatataaaa gaagttaatt aactaataaa aggagttgtg attaccacac
     3061 agctccttaa atctatttga ataaatatat gatattcatt tgttacactc tcaatcttag
     3121 ctgttgttct attcatcatt actcttattg taagtagttc agtcaaattt gcttgttcaa
     3181 ttgatcgata acagtatgaa ttgtttgaaa agtaatcgga tatataattg ttttctcact
     3241 acctaagcct tcttggtata aagaactatc gcaaagtata atccgctgag tacaaaagaa
     3301 agtgtcgtct actatttctc taagttgttt acaaatgata ttactaatag aaaagtgttc
     3361 tcccaaaatg tcttctaact gttgtcgata gtataaatac ctaaagtctg acggagctac
     3421 tatgaacact tcaattggag atttccgttt atgctcattt aaaagactta aactaatggt
     3481 tagtttacga atagaattct cattccaacg cagaaataaa ttgttttgtt ttcgccattg
     3541 ttcaagaatt tcatgaactt ctaagtacaa agcgcgttgc tcttcattta acaagtaagt
     3601 cttatcaggt tgaaatatct gaatatcagc ccaaagtgag cggataaacg ggagaagtgc
     3661 tttatcaaaa agtatctgat tttcttcaga tatattcaga gataagataa gtttttgact
     3721 gagctcaatc accaaagggt tatctgctac atggttttga taaacaacgt tcaagtcttt
     3781 ttgtaacaat tcaaagttat cgcatagata atttctagag ttgaatagaa agtagatata
     3841 aaggcattca tcttcgtcat gacataaaga gaaagaaagt tgctctaatc ctttctgtat
     3901 tagctgaaac aagggagtat gtcgggcaac tcttttttct tcctgactaa aatatattgg
     3961 gttagtctta gatctctgca aaccgatagc gatacctaat aaaataaatt gtttttcaac
     4021 tgtagggtaa cttcttgaaa aaaaatgttt ttctacataa tcaatcaatt gttgaccgct
     4081 ttgaataatt gatttattta gctggatcaa ctctgtccga tttgtctcat agattatcgt
     4141 tagtaacaaa cttcgataat ccttttcagg aatttcaaaa tggccatcag catttaagta
     4201 ctgcatttgt ttaaaaaaat taatcaactc atttcgtaag ttgtaaactt tgcttaacga
     4261 aatgaaggta gtatcagcta tattactcca attgtgttcc cctgataaat aatgtcctag
     4321 tacattgaaa aagtcagagc gctgatagat gagttgagtg agtgccaacc tcgaaaacga
     4381 agagtcgaat gcgactgtaa agctacttct agatttctcc actgaaataa tttgctgatg
     4441 gagtatgtcg gtaatgcatt ccaagtcgtt agtgacagac agtgagtggg caaacgttca
     4501 attgatctgc tatttcgcga atctgaatgg tccctttttc gtgtaattgc tcacaaatgt
     4561 aaacttgacg aaaaatactt ttctccagat aggtttccaa catagtactc ctcctagtca
     4621 ttttatacat aactattata actctaccaa tcggaatagt cctgtaatat attgaatcaa
     4681 aaatataaat tataaaataa ttagatgggg tatttggttt aagtatccct tatatagtaa
     4741 taaatttata ggtgtgattc tcgaattaat acccgaaaat gtgagagaat ttttatggat
     4801 tttttaggat acaaattaat tatttttgct tttatcctga ttaccagtcc agtactgtcg
     4861 gtatcccatt ttgcagcaaa atcatgttga taaagtgaca agaagactga tgagatatat
     4921 tatccaaatt gagaagataa ttaatcggat tcttgagtat accctacttt ttaagaaagt
     4981 agggtataca agcgtatgaa taaagacata actttccctg tgatggacgt tcggcttgtt
     5041 ccaattacca aaatacaggc gaacgattat aatcccaatc gagtagcgtc tccggaaatg
     5101 aacttattgg agctttctat cattgaagat ggctttactc aaccattagt ttgttattac
     5161 aaggaaaaag aagagaagta tatcttggtt gatgtgcttc ataggtatcg tgttgctaag
     5221 gaaagactca acttaaaagt tgtaccggta acagttattg ataaacccat ataagaacgt
     5281 atggcttcaa caattcgaca caatcgtgca agagggaccc atgaaataag aggaatggaa
     5341 attgtcatcc aaagattagt aaaaaatgga tggtcagaca agagaatatc caaagaatta
     5401 ggcatggaca ttgaagaaat ttttcgcttt aagcaagtgt ctg
//
LOCUS       DAYYFU010000111         5367 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000111 DAYYFU010000000
VERSION     DAYYFU010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.117"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            746..1657
                     /locus_tag="ACHA34_002715"
     CDS             746..1657
                     /locus_tag="ACHA34_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HFM8793295.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            2144..2410
                     /locus_tag="ACHA34_002716"
     CDS             2144..2410
                     /locus_tag="ACHA34_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793296.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2482..2823
                     /locus_tag="ACHA34_002717"
     CDS             2482..2823
                     /locus_tag="ACHA34_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793297.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2878..3297
                     /gene="ssb"
                     /locus_tag="ACHA34_002718"
     CDS             2878..3297
                     /gene="ssb"
                     /locus_tag="ACHA34_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8793298.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3374..3670
                     /locus_tag="ACHA34_002719"
     CDS             3374..3670
                     /locus_tag="ACHA34_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793299.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3685..4116
                     /locus_tag="ACHA34_002720"
     CDS             3685..4116
                     /locus_tag="ACHA34_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM8793300.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4421..4603
                     /locus_tag="ACHA34_002721"
     CDS             4421..4603
                     /locus_tag="ACHA34_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793301.1"
                     /translation="MQRTLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKR
                     FTEEIEQLAELNIRYF"
     gene            5007..>5367
                     /locus_tag="ACHA34_002722"
     CDS             5007..>5367
                     /locus_tag="ACHA34_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8793302.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNE"
BASE COUNT         1981 a          787 c         1116 g         1483 t
ORIGIN      
        1 aaaaaataaa cgtattttgt ttgaaatccg tttatttttt ttacgtttca gtattagtaa
       61 ggattatttg agggttattt agagattatt taaaggttat atgtaataag tggggggagg
      121 gctgtcggcg cttggtatga aaggcattct ccgacttcca ggtttttgac cattttctct
      181 caacgtggaa aaaccgccac ttatgagaga aaatggtcaa aaacctaagc gggaagtctc
      241 cgaataacat accaagcgcc gacagccaac agataaagaa tacactacta aaaatagtag
      301 tagcataaaa tttaaaaggg aaaaaatcaa cccctacccc agtcttgggg tctcaaaact
      361 aactcccccc taaccccctc tttcagcctc ttttgaaaga gggggagaat caaccccacg
      421 gccaacgccc cagtgttctt ggggctgtcc agaacagaaa cttgcgagag agtgggggta
      481 aaacaagtgt agaggaaact tgaagacttg ttcgaacaga aacttgcgag agagccggag
      541 taaaacaagt gtagaggaaa cttgaagact tgttcgaaca gaaacttgcg agagagttgg
      601 agtaaaacaa gtgtagagga aacttgaaga cttgttcgaa cacaaactta caagagaggg
      661 gagtaaagca gttgcagagg aatcaggaag atttgttcga acagaaactt gcaagaaagc
      721 attattaaat tgaaggtgat gaaatatgaa gtatttattg catggctatt ttattgatta
      781 ttctactcgc aatagtagta ctttagcgat tctatgtttg cttcatgaat tgagagtggt
      841 tactagaaaa cagctataca aactattgaa ttttgacaga tcaattagta aacaaggagt
      901 aacgaatgct ttagtgagat tatatgaaaa acagttaatc gatcgaaaaa aatatggaac
      961 agaaatgtat tattatttga cgaaagaggg acatcaaagc ataggtgggt attatacgtt
     1021 gccgaaagtt cctgaatata atttgaatca ccatttacaa attaacaatt atttgattaa
     1081 aggaatggca ttggcaaaag accacccaca tttaaaacgg attatttcag aaagacgtac
     1141 cgtctatgaa acgaaggatt taaatcctaa gacaaaagga agaaaatttt ttgtgccaga
     1201 ttttattttt cgttttcgag acaaagaaaa tcatgagatc aattggtcct ttgaaattga
     1261 gctaacgttg aaaacacgtc gtcgctaccg tgaagcgatt tttccgaaat atattcgtga
     1321 gttgcgaaca cgtcctgaat cacatttaat ttatgtgaca cctagtggcg caattcaaga
     1381 agaacttcat gcgtttaagc gtattttttt caagcgcgaa actgaaaaag atgcgagtca
     1441 acaagaattg tttgagcggt tgcatatttt ttcagcttta gagtttgaag agggattaaa
     1501 agcattatta gagaatgatc cagcagtcaa ttggcggaca gtagaacgag aaaaaattta
     1561 tccgaatggc gctgttcaaa gtcgtttgaa tttatttgaa gaatcaactg aaaatgagga
     1621 aaaaccaaaa gtgaagttta cgcctgaagt attgtaaaag agggtgttcg cttcgctctt
     1681 tttttgtgcc agtttgctgg caccgttcag acgcaagcgt ctgaaccggc tcgtttttag
     1741 gaaggactaa cttgtttgat ttatccgttg tcaaagaagt ggacgttcga gagtccctaa
     1801 tcgtatagca gtcattcggc gagtataacc taaaacataa cctgccaatt gccaagggta
     1861 aaatgcacgc tgcgcgccct tggcaatcgt cagaacatgt tttcgagccg gttatgccga
     1921 acgaccgcta tacgaaaagg gcctctaaac agaagtccac tttgatcctt gacaactgaa
     1981 acccaaaatc aaacaagcag gccttcgtct aaaaacgagc cgaatgaaga ccttgggggt
     2041 tgcaccccca aacccccgtg cccccgacac actaccgaaa aggtgggggt gggtggcgaa
     2101 aaaatcaaat aaaacattct attcaagaaa ggaagaaaca accatgacaa ctaaaaacga
     2161 gcaactgaaa gaacaggcaa gaaaagagtt gcaagaaaga ggtttgatta ttgaggggag
     2221 ctttgaagga gattttgaaa cctatattgg ttgttatgca agaccgataa acaaacctac
     2281 agccttagac ccaatgaacg aacaagaagt aaaagaacaa gaaaaacatg cagtgaatgg
     2341 ttttccgcaa gatttcactg aatggtatga gtgggaaatt aacaacggga agcttgaaaa
     2401 cttcttataa aagtcagatg acgtgcaaag agaatgattc tctttgtacg gtaaaaatat
     2461 aaagaaagaa ggaaaaagaa aatggcaaaa aacacacctt atcaagtagg agacattttt
     2521 tatagtagtt ggggctacga acaaacgaac gtgacttttt ggcaaattgt gaagttaaca
     2581 gaaaaaacag catggtttca gcctattcaa aagaaaacgg tcgagcatta ttcgtccatg
     2641 agtggaaaag ttgttccaat gaaagacaag tttatagagt atcctttagc aaaaactaaa
     2701 gacagtattg taaaaaaacg aattgatcca gaaacctatc ctgatatgtt atgtggttat
     2761 cgtagttggg acacgtttca tagatataaa aatcaagctg tttatgaaag tagttattat
     2821 taaggggata gtttcccctt tcaaaaataa taaatagaag aatggagaga aaaaatcatg
     2881 atgaattcag tgaatttagt aggacggtta acaaaagaag tggatttaaa atacacacca
     2941 aaaggcaatg cgacaggcac atttatttta gctgttaatc gaaattatac gaatgcaaac
     3001 ggcgagcgtg aagcagatta tatccgctgt gtcatttggc ggaaagctgc ggaaacttta
     3061 gcaaaatata ctggaaaagg aagtttgatt ggcattaatg gacgtcttca aacgaggagc
     3121 tatcagaatc agcaaggaca gaccatgtat gttacggaag ttctagtgac agatttctat
     3181 ttgttagaga gtaaagaaat caatgagcaa cggtcaaaat caatggcgaa taatcaaaat
     3241 aattcttcaa ttccaggaaa tgagattcaa atttcagata gttatttgcc cttttaagtg
     3301 aaaaataatt ttgttctata cctgatttct tcaggtatag aacaaaaaat aaatagagat
     3361 gaggcgattt tttatgaaaa tcattgacag ttacgatcaa cgaaccatgc aatatttggg
     3421 ctcgtttgat tatacagatg aaaatattat taattatgtt gcaagtttac ctccttatca
     3481 aagcgttcgc ttggtagatt ttgcaagtga tgaattggta ttaacaacga tgggcaactt
     3541 tttagattat gtcggagaca aaaaatggtt ggaaaaaatt ttgtctaaat tgattgcgaa
     3601 acaaacagga gaagaaccca taaatgcgat tcaattgttc aatcgatatg aacaagaaaa
     3661 caatgattaa agaggagaga aaaaatgaaa agattaaaac gaagtatttt tattgtgcta
     3721 gcattgattg attttgttgg gacaggttgt gaagcaattg acgttcaaaa agctccctta
     3781 tcggaaataa aacaagcaat aacaaaacaa gatgaagaac aaccgctaaa tacacaagta
     3841 gaaatagtga atcaaaataa accgagtttt tctgaagaag atttttcgtt agaaaatgga
     3901 accaggcaat ttttttcaga tttggatcgt ttaaatcggg taggtgtagc aagtgcactt
     3961 ttgcataaaa gcaggatgcc aacagaagaa cgagaccgat tatacattaa gccaactggc
     4021 tggaaacaaa agaaaatgaa gaatggcgat tatctttata atcgttgtca tttgattggc
     4081 tatcaacgtg cgacaagttt ccccataagt atgtaatacg aaacgggagg attaatctta
     4141 agattaatca caactaacct atttgacagt tggaatgaaa gctatcatgt ctgctgaatc
     4201 aactggtctg atactcctac atgcaataaa gtctactagg aagttattgt atgaagctag
     4261 gtaaaggcgg cagaacaatt tctaagtgtg gaatcatatg aagttggata tgccgggacg
     4321 ctataaatta ctcagggtga gactgttttt aattaaactg acgaacttgc gaatgtacgg
     4381 gtctataacc gacttatatt cgaaagatat aagattgcga atgcaaagaa cattatcgaa
     4441 aagtaagaat gttggtcatg aaattcgaga tggaagacta gcggtgtatc tgtcaagagt
     4501 tggtaaaaca tgtttgattg attcagatga gagtgggaat gcgacaaaac gttttacaga
     4561 agaaatagag caactagcag aactgaatat acgatatttt taaatacttt agcggagaat
     4621 aaaaaaatcc tctacagaaa actacggagg gcaacatgtg ctattgtttc aacaatacat
     4681 ttttaatttt taccataaca aaaaagtact tgaaaaaaaa cagaaatatg ttaaattaaa
     4741 aatgaaaaaa tggaagcggt tacttattct tttgaatcta agataaaaat tcaaaagaat
     4801 aacataaccc aaatatgatt atacttgaga ttctaaatat ttatcgaaaa ggaatttgtt
     4861 ttaaggaaaa atgattctat ggacggttat tgatttatat ttaccaaatg gctaataaaa
     4921 tagtatttct tatattgtat agtttttgtt tttatgtgaa aacatgagca atagaattgt
     4981 actagtagga taaggatgga tggaaaatga aaaaaactat ttttgtttta gcgattttta
     5041 tatgttttgg gctacttttg gtaggctact tgttgtttaa ttctaatgaa atgagaacta
     5101 tttctacgaa tgacttgtta aaaaaaagtg aattaaagga taaaaaaaaa gaatctcttg
     5161 cctttataat gtttactcaa gaagagtgtg agtattgtat agcaataaat ccaatcatag
     5221 atagtgtaaa taaggaaact ttgaataaga taaagaaata tgatatatct gtagattttc
     5281 aatctaaaca aaagtgtaaa attaagtttg ttccgactat tgttgcatat agtaagaata
     5341 aagaagtggg gagaattgac aatgaat
//
LOCUS       DAYYFU010000112         5341 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000112 DAYYFU010000000
VERSION     DAYYFU010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.118"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..285)
                     /locus_tag="ACHA34_002723"
                     /pseudo
     CDS             complement(61..285)
                     /locus_tag="ACHA34_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(839..925)
                     /locus_tag="ACHA34_002724"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="ACHA34_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2091..3131
                     /locus_tag="ACHA34_002725"
     CDS             2091..3131
                     /locus_tag="ACHA34_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8793303.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            3955..4287
                     /gene="traE"
                     /locus_tag="ACHA34_002726"
     CDS             3955..4287
                     /gene="traE"
                     /locus_tag="ACHA34_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFM8793304.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4906..5247
                     /locus_tag="ACHA34_002727"
     CDS             4906..5247
                     /locus_tag="ACHA34_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFM8793305.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1943 a          728 c          900 g         1770 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt taagattaat ttttttatga cttgtacaaa tttatctgat attctcttat
     1381 taagaactac ctaatttggg tagttcttat tttttacatt ttaagtagta tttaattgca
     1441 atttggtagt atttttttat tatcttattc ttagaacggt agtacttaat ataaatatag
     1501 gtagtatcta atttaagtat taatggtagt atctaaatta acaggtagta tgtaactact
     1561 ttaaagtaat ttttttcaga ttaacacagt agtatcttat taaataagtt aattttaatg
     1621 gtagtacata attattatat atatctgaca ttggtagtac ttattgtatt ttttagtagt
     1681 atataatcta ttgttttcta gcctaaagcc ttgtcttata agaataatat aatatatact
     1741 actgaaataa ggtagtatat attactagta atggtagtat gttttatatg cataaggtag
     1801 ttcttaattt attttttgtt ttgacactgg tagtacttaa ttatctttaa ttttaaaact
     1861 aaattttatt catacgtttt tctggtagta tttaattata aataatattt atttaaagta
     1921 gttcttaatg actttaaagg tagtacataa ttcattttat ttctgatttt aggtagtatt
     1981 taattaaaaa acagttttaa tgcgtttgca ttttttattt atctttgcta ttttcgaaaa
     2041 gagtaggtaa ttttacataa ataaaacaac gtgaaagtag aggatctaca atgactgatt
     2101 ttaaattttt taaggcggat agagtttaca acgaattatt ttatcaattt ccaaaagtct
     2161 ttattgtttc tgacgaatac aaaaaaatga aagattcaac taagattgcc tatatgcttt
     2221 taaaagcaag attagagatc gcaatcagca aacggcaaat cgatgaagaa ggtaatgttt
     2281 attttactta tacgacaaat gaactatgta gagtattaaa ctgccaaaaa caaaaagcga
     2341 tagcaatcaa aaaagagttg gaatcctttg gtttattatt acaaaagcag atgggattta
     2401 acaaacagtt agggaaaaat aatcctaata gactatatct agcagaatta aaagtctcag
     2461 aaaatgatat ctacttactc gaaaaatttg atagagagaa tagggaaaac gttgataaat
     2521 cagagggtat gaaaatcata cccaccctcg acgaaaaatc agacgctgaa tcccttgggg
     2581 ctcaagaggg tatgaaaatc ataccgtgcc aaaacgttga taaatcagag ggtatgaaaa
     2641 tcataccaga acttaataat aatatattag acactaatag acacaatata gacactgaaa
     2701 aagaccgcct acaagatcaa ttgttgttag acaattttga gacaattatg acaaacgaca
     2761 gcattgctac gtttgtccct gaacgagtat taaatttgat aaaaacattt tcttcaagtt
     2821 acagtgaagc tcaaaaaacc gtccagacta ttcataatgc aaagaaaaaa gctgaaatag
     2881 aaagtggtat ttcgatagtt tttgaagaac tcgatagtta ttatgtcaat gcagaacaag
     2941 aattatacac gacactgtta aaagcctatc aaaaattaaa aaccgaaaaa gtcgaaaata
     3001 tccagaacct gatttttgtc tatgtaaaaa attggtttat cgaaaaacca atagctgcta
     3061 aagtatcaag tgaaaaacgt ttgaattatg aaagctcccc aagcactatt acgaaagact
     3121 ggttagagtg aaactttact gacaaagtga ttgttgcttc atggagtaac attaaaatta
     3181 aaaatttttt attattctaa ttatattacg aatattacaa aataatagct attttaatag
     3241 aatttataag ttaaaatatt atgataaaaa tcacaaacgt agccttttac gtcataagtg
     3301 ttaaaagaaa taattaattt caaatataga aaagggaacg tatgtttgca tactcctttt
     3361 ttttgagtta taataatacg cgaagagaga taatttcatt atttaaacaa gctaaaaatc
     3421 ttgatattac ttcttttcag gcaatacgaa tattattcgc ttacattttt tgtataaatt
     3481 taattgtctg ctatgcttgt gatgaattga ttttcgttct gcacttttgg atgataaatt
     3541 tggacaattg aaaatacaaa aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt
     3601 cgtcagcgtt catacacggc attaagattt ggcccttaat gcgttaacct ttcagtgtta
     3661 cggtattacc cgcaatggat ttagtataac agatattcaa aagtgatacc actttttttg
     3721 gctgtgttaa ggttaaaaat acgtgatatt acgatgaatc tatgcctaag cacaatgtta
     3781 taaaaatgtc cgtgtggctc tttgccgtat gacgaacgct gaaattagag ggaacgcatg
     3841 tttggatgcg ttccctcttt tttgtttgtc atgaacgaaa atcaattctc tatttttgaa
     3901 aaataacaag aaaaacttgt taaaaatatt taaagaaatt gggtggttta ttttatgtca
     3961 tatacaggaa ttttgagtct caaagatatt tgtcactacg gtaaacggtg taccgctaca
     4021 gagaaaatca cgaagaaact atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa
     4081 tatataatcc agaaagacaa agtctcagag gaaatgattt attacattgg gaaacaaaag
     4141 caaataattt taaaagatcc tatcccctta aaagagctat atccaacaat caaacatgtt
     4201 tatgaccaaa acggtgtact gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca
     4261 aaagggatgg ggcgtttgat cggctgaatt gatcattctc ataacttgta ttttatcgtt
     4321 tttcgcttgc ttgaaaatat tgtcgagact tctaaaggaa cttagagtca ctagaaaaat
     4381 aaggagtaga cttgatctat caacaattcc cttcggggag tgacggggta ggttttaacg
     4441 attggtaacg taaaaaattg gtcaatcgag taaatgcgct tgtttggata gtggtgaaat
     4501 gaatttgtgt aaataataag cgcagagtaa acaagaggga atttagaagt tagacaagtt
     4561 agaatagcgt ctagattaat catgagcagc tactaaggga tctagacttt gacgcaaact
     4621 catggaataa ggagtaggtc ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt
     4681 gctgcagtac atgtagatac ttgcgaggac gttgattatt ggatcaatcg agcatttacc
     4741 gctgtctact agggtttctc gtttcggacg ccataaacga gtcgaaattt tttatgtatt
     4801 tttaagtaaa cgaaaaacga taaaatatga acaagtcctg aataaagtga aaaaattgat
     4861 ataaaacata gagaaaatga aatttacgta atggaggaat gacaaatgaa aaattatgat
     4921 ccgaatatac gattgggcac acatactatt aaggtttctt tccaacgttg ggattacaaa
     4981 ggatttgtga cgtttagaag aggcggaaat tgtaaaggat tagatgtttt agcattagac
     5041 gaggatgatt tatacgatca aacattgact gataatccaa ttggattcgg cttgctacct
     5101 gaagatgatg aaggtaatga atggtttaaa atgacgctta tgaatgataa tggagacgaa
     5161 ttatctgttg aagatacttg gtcatatttg agtgattata ttgtcagctt tgaaatcatt
     5221 gaatttgtag cggataaaga agaataaaaa ctggaggaat aaattttaca gataaaggct
     5281 tagaaggagt cacgatgatt aataaaaagg tagtattacc ttaggcattg agtaaggaat
     5341 t
//
LOCUS       DAYYFU010000113         5261 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000113 DAYYFU010000000
VERSION     DAYYFU010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.119"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..354)
                     /locus_tag="ACHA34_002728"
                     /pseudo
     CDS             complement(1..354)
                     /locus_tag="ACHA34_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(614..1162)
                     /locus_tag="ACHA34_002729"
     CDS             complement(614..1162)
                     /locus_tag="ACHA34_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793306.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1163..2020)
                     /locus_tag="ACHA34_002730"
     CDS             complement(1163..2020)
                     /locus_tag="ACHA34_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8793307.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2306..2593)
                     /locus_tag="ACHA34_002731"
     CDS             complement(2306..2593)
                     /locus_tag="ACHA34_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8793308.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(2583..2912)
                     /locus_tag="ACHA34_002732"
     CDS             complement(2583..2912)
                     /locus_tag="ACHA34_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8793309.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2989..3054)
                     /locus_tag="ACHA34_002733"
                     /pseudo
     CDS             complement(2989..3054)
                     /locus_tag="ACHA34_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            3090..3659
                     /locus_tag="ACHA34_002734"
                     /pseudo
     CDS             3090..3659
                     /locus_tag="ACHA34_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259634.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
     gene            3794..4346
                     /locus_tag="ACHA34_002735"
                     /pseudo
     CDS             3794..4346
                     /locus_tag="ACHA34_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015474649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            4498..4731
                     /locus_tag="ACHA34_002736"
     CDS             4498..4731
                     /locus_tag="ACHA34_002736"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8793310.1"
                     /translation="MFQSFIHEKQRELSIKMIQALKTFRQSEEAIVNALNYHYSNGLV
                     EGINNKIKVIKRTAYGYRNFSNFRNRIFIEREL"
     gene            4816..>5261
                     /locus_tag="ACHA34_002737"
     CDS             4816..>5261
                     /locus_tag="ACHA34_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFM8793311.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDSDTLPMKGKRKPNGHQEKRGKQQYQRSIHDR"
BASE COUNT         1652 a          996 c          841 g         1772 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat acttatttga gagtcatcta attggctcat
      241 aatcctttct ttttggttag tatctgcagt tacttgttta gtatgagcca tgaacccaaa
      301 catactcacc ccaacaacca atcctaaata tattttcttt ttaaactttt tcatctacat
      361 ctcctcctaa tagttttatt ttagagttag ctctattact tatttactaa ctcaacaaca
      421 gattatcata cacttgttaa tgaaatcaca atatctcatt aaaacacacc ctaaaatcat
      481 aaccgttcct atgctcattt tttggatttt ttaatcacgt aaccagaaaa ataaatcccc
      541 tctatttcac gcaaatcaaa gaagaactaa gtaactaccg tttaaaggta gttacttagt
      601 tcttctttga ttattatcta actttattat tcattcccca ccctttgcgc agtgagtctt
      661 cggtatcttg ttttatctga ctggtagttc ttattatttc tccacgatca tctaattctg
      721 gaacaattgg atatttttct ctgaacaagt ctagttcttt caccacgtca ttaaacaaat
      781 cttgcttatc ttcttgcgta agattctgtt taatatactc atctaaagct tttctaagca
      841 attcatctct tgtcatatta gtctcatact tttctactaa aactctggaa gtcgcattta
      901 acaaagtcga cagttttaaa ggatgcttgg tggaataaac cttatcaaac tttctgattg
      961 gtgcgccaac tttttttgtt cttcctctac tctgtctttc aacattggag gaagttttta
     1021 tttcttgaac ttggtgcact tctaattgct tattttcaga tggtgtaatt gcttgctgct
     1081 ttttctttgc ttctgcttct ttttttattt tggcgagtcc tttgttgttc ccaaaaatat
     1141 caagtccttt gatgtcagcc aattactcca cctccgcata ccaattctcg tgttcttcca
     1201 attctttcaa aatactgata aataattcgt ggcttttgcg atcccattgt tttaagagac
     1261 ctttctttgt cttctccatc gtaatacctg atccgtcgta tcgttttaag cgcgcatttt
     1321 tgagtacaac atttttaatc aaatgttccc cataaatctc ttgggcttgt ttatacattt
     1381 cctgatctac tgcgtcaccg ctttccaaca tgaaaggaat aacaccagca acatataaat
     1441 ttgtatcaaa ttctttcaca aaaaagtcca tatactcaag atattgcatc gcatttctca
     1501 tcgattttac ttgggtctgt aagacgacga ttgcccagtc cgcaatgtac atcgcggatt
     1561 tactatatac agaaatagaa ggtgggacat ctaaatagat gacgtcaaac tcatcaaata
     1621 atggttctag cttttctttc atatatttga attgggataa tgaatcatta tattggtctt
     1681 tgatccaatc atcatacatt tcaaaggaca tgtctgctgg aagtatatag aggttattga
     1741 ttactttata gacagcctct ttaggtggaa gattgtttat caacacgtca gtcacagtat
     1801 tttcgtaaac aatcggttca ccttctccgc ccccttcttc gttagtgaag ttcatatgcg
     1861 ttaagttcat cacatcagaa gtatttcctt gcatatccaa gtcaattatc aaggttcttt
     1921 ttccttcatt aactgctgat tcaaacccta acatttgcgc gcacgtactt ttacctacgc
     1981 cacctttaaa attaccgatt acatatttct tcgctaccac gttcctacct cttttctttt
     2041 agcaatattc atttgatact attataacat atttattttt agttactacc aaaaaaaaga
     2101 tatatatgta ctaccttata attaataagg aaaaggtagt acataaataa ggaaaagctg
     2161 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
     2221 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa aagaagccgt
     2281 ccttcttaag aacgacaaca aatcactaga ttaatttttt caactgttca taaaagtttt
     2341 catgcgttcc acataacaat actggaataa ccttgttttc tataatctcg taagctactc
     2401 tatatgttgt gcccgcatat ttgaacccac tagaccaaat acctgataaa tccccagttt
     2461 tttgttcacc cttatatgga tcaatcgcaa tttcatcata aattaaattc aaaaattttt
     2521 ctttcaatgg cttctctttt agctttttta aatacttaat gacatgtttt gaaggaacga
     2581 tttcataagc caatttctgt tgccaactcc tctttagtca ttactttaga attagtaagt
     2641 gtatcttcag caatatctct aaaggaagaa tgcattttgt tttttctatt cttaaattct
     2701 acaagaatct catctttatc gtagccttct gcaattatat cagacaaaat atcttcgcta
     2761 aaatcataaa attctttttg tggttcaaca aattcataaa gaataccatt ctctaccaac
     2821 tttgcttcaa cttcgactcc attaggaaca ttaaaatctt taggtactgt caacatgatt
     2881 gaattgcctt gttttcttgt ttttgtaatc atttaaatcc ctcatttcta acagtatgta
     2941 attacttagt aaatacatta taccacgaga taatgtattt acaagtaatt actgtgactc
     3001 gtaatgcgtc cttgctgaat aaatttcaac cacgtgttca tcttcatcta ccttatttga
     3061 tagtagattt aaaactttgc aacagaacca ttgtagctat tggggcaaag acttattcag
     3121 ctcactcgat tgccggcagc attgaaagtt ttgtttatat gccagcttat ggattagcta
     3181 ccgctgcagc aactttagca ggaaatagta ttggaaaaaa agactatgtg gaaacaaaac
     3241 gtgttgcatt ttatgcggtt aaatacggcg tgattgtttt aagtatccta ggtataggat
     3301 tgttttttgg tacgccatat attgcacctt ggtttaccac agatgctgag gcaataaagc
     3361 aaattgttat tgcgctaaga attgacgctt ttaatcaacc aggactagct atcagtctaa
     3421 tattagctgg cgtactccaa ggaatgggag atactaaaac cccattatac agtactgcat
     3481 ttggaatgtg ggtcacacgc atattaggtg taatactttt agggaatata ttggatttag
     3541 ggattgctgg agtgtggtta gcaattggca tggatctata tatacgttca ctttttttaa
     3601 tctatcgttt cagacgaaat atccaaattt taaataacaa taacgaaaca ccttcctaaa
     3661 ccagtgattt tttatcgctg gtttttgcca tttagcgatt aaaaaaattg ccatataaat
     3721 agaaaagtaa cacaaggggt acaatggtgt cataactaat ggtcataagt gaagtgaaat
     3781 aaggggtgaa aacatgaaat atggctatgc acgggtgagt acccgccacc aagatctgga
     3841 agggcaaatg cgtcagctgg aagaggaacg ctgtgataaa attttctttg aaaaaataac
     3901 gggaacgaag agtgatcgtc cagaattcca aaaactgctt caggaagttc agactggtga
     3961 tacgttagtg gtcacaaaat tagaccgatt tgctcgtagc acgcaggatg ctcttcatac
     4021 aatcaagctt ctttttgaaa aaggcgtgcg aattaatgtt ttgaatttgg gcattattga
     4081 gaacacgtct actgggcgat taattattca ctatttttag tgcctttgcg gactttgaac
     4141 gcgatttaat tgtggaacgg acacaagagg gaaaagaaat tgccaagcag cggcccggtt
     4201 ttagagaagg gcgaccaaaa aaattttcca aacaacagat gactttagca atgaaactac
     4261 tagagacaaa ttcctataaa gaagtagaga aaatgactgg tattagcaaa agtactttaa
     4321 cgcgttataa aaggaagaag agttaggagc cacagaatac cgatatcatc gtcttttcaa
     4381 aggcatggtg acggaaaggg gaatcgttga ttattttctt tccttggacg aaggtttacg
     4441 ccgccattac aacgtctacc aaacaactgt ctttgccatt aactatcgga accgaaattg
     4501 tttcagtcct tcatccatga aaagcaacgg gagctttcaa ttaagatgat tcaagcctta
     4561 aaaactttcc gacaatcaga ggaagccatt gtcaatgcgt taaattatca ctactccaat
     4621 ggtttagtgg aaggtatcaa taataaaatc aaagttatta aacggacagc ctatggctac
     4681 cgcaactttt ccaattttcg taatcgtatc tttattgaac gcgaattgta aaattaagtt
     4741 agaaaaataa aaaggcattt atggtacact caaattgtat ttccgacgaa agaaaacaaa
     4801 ggagtgtaac cataaatgac ctacaaacat cttaccatag acgaactgac aatgatagaa
     4861 tcatattatc ttcaacataa taagccggtt gaaatcgcta accgaatggg acgtgctata
     4921 caaactattt ataatgtagt caataagttc aagcaaggta agactgctct tgattattgg
     4981 caccagtata aagaaaataa gaaaaaatgt ggtagaaaag tcattcaatt acctgctcat
     5041 gaagtagatt acattaaaga gaaagtcact cttggttgga cgcctgacgt cattatcggg
     5101 cgaaaagaaa gacctgtttc atgcggtatg agaacacttt atcgtttatt ttctaaagga
     5161 atatttgata gtgacacact accgatgaaa ggtaaaagaa aacccaatgg ccatcaggaa
     5221 aaacggggaa aacaacagta tcagcgctca atccatgatc g
//
LOCUS       DAYYFU010000114         5220 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000114 DAYYFU010000000
VERSION     DAYYFU010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.120"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1256)
                     /locus_tag="ACHA34_002738"
     CDS             complement(<1..1256)
                     /locus_tag="ACHA34_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM8793312.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
     gene            complement(1270..2487)
                     /locus_tag="ACHA34_002739"
     CDS             complement(1270..2487)
                     /locus_tag="ACHA34_002739"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM8793313.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(2477..4072)
                     /locus_tag="ACHA34_002740"
     CDS             complement(2477..4072)
                     /locus_tag="ACHA34_002740"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFM8793314.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            4197..4982
                     /locus_tag="ACHA34_002741"
     CDS             4197..4982
                     /locus_tag="ACHA34_002741"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HFM8793315.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            4975..5217
                     /locus_tag="ACHA34_002742"
     CDS             4975..5217
                     /locus_tag="ACHA34_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793316.1"
                     /translation="MTNNILISIYPRYMEKIINKQKNFEFRNFYLTSDPYNIFWIYET
                     IPTKALKYKLIVKNPITKLKKNQHYFLGDDEFEQLV"
BASE COUNT         1574 a         1051 c          862 g         1733 t
ORIGIN      
        1 actacaaaca cgatatgttg ccccattaat ttttggaagc gattggttcc ttttttctct
       61 tcctgtttct ttgcatattt caacgccgca tctagttttt gacgggttgt aatgatcaca
      121 acattcgcat tggcatctgc caacagagtt tcacttaact ctttggcact tcctgttccc
      181 acgatcaagc tattcgcttt ggcattacca gaagaaaccc cagtattaaa ctccgaagca
      241 aatttcgtaa actctgtggt ggtctgatta tctaaatctt tgcggtccac taacatgatc
      301 gttcgatcga tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc
      361 tttcctgaac cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac
      421 aaggcttgaa tcgcatgcac ttgataagga tgtaacacca tgagggtttt attgtcttga
      481 tcttcactga caatcgtata atccgcaatc aagcgatggg catctgggat atttagcact
      541 tgtttgcaaa actcatataa gttttctacc tttttattat ctttcgttcg ccaactaaat
      601 aaaaacttag gatgcaaatc cttcggtaag gcatttgcaa aataccgagt cgtttgttca
      661 ttggaaacca caaacaactg taaggtcgaa aagatattgt tccgaaacat gccttcttca
      721 gcatattttt tgatttgatt aaacgcctga taaaaaccat cttttgcagt tacttgtttt
      781 agctcgattt ggataatcgg taagccattg atcaataagg tgacgtcaaa acgtcgatcc
      841 cgcgcttcta tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt
      901 cctccaccaa tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcggattt
      961 tcccgttcaa tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca
     1021 aatggggttt tcgtccgtag caataattcg gttttgatcg tttcaaactc tttatcggtc
     1081 aaaggagaat cattcagttc tgcttgatta tttgagatga ttttttgacg taaattgctc
     1141 caaagatctt cttctgattt tagatccgga cgatacgtcc actgattatg tccttccccg
     1201 aggacacgaa tcaactgttc ttcgacttcc gcttcctcac ggtgactaat tttcattctt
     1261 cttccctccc taaacaaaca tcttttgcaa gaagcctttt ttcgtttcct tcagcaaatc
     1321 taacttacgt tgatgaagag cgatcgtatc atctagctgc ttgaagaagg caccaatttt
     1381 ttgttgttca tttatatccg gtggaaactg aaatgtcctt tgttctatac cacctttagt
     1441 cacatgtacc attgtcgttc cgttagttga ttctttaatt ttctctttat cagtcaatag
     1501 ccaagtatat agatactgtt tgtccaattc cttagataaa aatttaagtt tccaaatatg
     1561 ataatggtag attacacgtt cttcattcca catttcagga ccgaagcttg ttgcccataa
     1621 atataagaga tctcctttat tagcatactt gtcttcctct aattctaagt ttgaataata
     1681 ccatctatca ttagtattaa aattacctac tcttaaaacc ctatattttc cagaatcaag
     1741 taattcttgt tgtttaaaag ctctaccatt caaaaaaact accacttccc ccaacttccg
     1801 ctcttcccaa tctcccgtaa atcctggaaa acgaacttct ggcacttttg ctccgttttt
     1861 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta attcgtgctg
     1921 gtgaagcatg atggtgtgat caagttgttt gaaaaatgaa ccgatcttga tctgttcgtc
     1981 ataagttgga agtgagatag gtgtcttttt accactatca attgtatagg ttccgacggt
     2041 tccaatgcct gcaacgtcac ggatatactt ctgaattttg ttatttatga acgagtagta
     2101 aaagaagttt ccgtcaattt tatgctcatt cttgaaccaa atgatgtttc catccttaaa
     2161 gtaaattggg ttatcatcag tgattaataa ggggacacct attgatccga caccagttac
     2221 aagcaagtct ccttgagaaa ctttacctga atttttcgaa tattcattat atttttcttc
     2281 tgaaatgtaa agataatcgg atggttcctc attttttgca gctgagacaa tatcacgagc
     2341 acgtaaaaat ctaatacctg agttagtcca atctgattga tgaattcgct tcacagaagt
     2401 aacatccatc atatctccta acttacgctc ttcccaatct tccgtaaaac caggaaaccg
     2461 gatttctggt cgtgtatcat tgctcatgtt tgaacacctc cagcgcacct tgtaaccacg
     2521 cttcattttc aggtgttgtt tgcagggaag agatagcttc taaaagttct ttttctagca
     2581 cttgctgttc ttgtcgtatc ttcttgatgt catttccgac atgaaccata tcaattggtt
     2641 cttcttcttc gaaagtatcg acatagcgtg ggatatttaa gttatagtca ttttctttga
     2701 tttcatcaaa ggttgccaaa tgagcgtatt tctcgacatc ttttctttca gcgtaggttt
     2761 cgaggatttt ttgaatgttt tcttccgata atttattctg atttttccct ttaacaaatt
     2821 cacgactagc atcaatgaat aatacatctc gagtttgacg attcttcttc aatacaatca
     2881 ccgtcgttgg aatcgatgta ccaaagaaca agttcgcagg cataccgatc acagcataaa
     2941 tactgccgtc ttctaataat ttttgacgaa tgaccccttc tgcagcacca cggaataaaa
     3001 ccccgtgtgg caagacgata gccatagtcc ctgtttcctt caaatgatag aaaccatgaa
     3061 gaagaaacgc aaaatctgct ttggattttg gcgctaattt cccgtagcga ttaaaccgag
     3121 aatcatctaa gaaggtcgta tctgctgacc aattagccga ataaggtgga ttcatcacta
     3181 ctgcatcaaa tgtgtaaggt tcatctgttg gccagtcctt attcaacgta tccccattgc
     3241 ggagattcat ttcttcggca tctaccccat gtaaaatcaa gttcattttt gctagattgt
     3301 atgtcgttgt atttagttct tgcccatgat atttaacgtt atctggatga gttaagtagt
     3361 tacgtacatt taacatcaag gaacctgaac ccatggttgg gtcaaagaca ctaaagaatc
     3421 gtcgttcttt ttgatccaac gtcacgattt gtgccatcat atcagaaacc atatgaggcg
     3481 tgtaaaattc tccggctttc ttccctgctt ctgaagcaaa ttgactgatc agaaattcat
     3541 acgcatcccc gatcacatcc ccatcgtgac ctaaaacttc cacgtcattc aatttcttga
     3601 taacttcggt aatcgtcaca tttcgttgtt gctcatctgt tcctagtttt tttgattgga
     3661 gatccacatc atcaaataac ccattaaatt gattgtaggt agatgccaat tgcacaaagg
     3721 ctttgtttag atcgttcaat tggaacgttg cttgttttgc ttgatccgct aagacgttga
     3781 aaagatactc tggtgagatc gaatacccca aaatatcaac gattgtggca atcaggtctt
     3841 cttttgattc ttcatctgaa agtaattcac gatacaacat tgtttgtttg cttactgtgt
     3901 catattcctc taaggattca tctgctaaaa gaaccacttg ttccaacaaa cggtcggata
     3961 aatatttata gaaaatcagt cccaataaat aatttttata ttctgacgca tccattttac
     4021 tacggagatt atctgccgca ctaaataatt tttgatttaa ttctgcaccc aaaaagatac
     4081 actcctatct cttatttaaa gttgctatat accctcttat tttagcatac tcccttttag
     4141 aaaaagcaaa aaaaaaagaa aaaatcttat tcttgataac aagctacgtg acgtatatgc
     4201 ctaacaaaaa tagaactttt gaatcaatct taaatgaagg tttaaccaca gaaccggcat
     4261 ccattatttt tgatgaagtt atcgatgacg ttgaagatag taagtatcat aaaaatttaa
     4321 aagaactttt ttattttgca ctcaacaatg gaccaagttt gttcactggt atatcagtac
     4381 ctgatagtga taatccatta attatggcaa aaatttactt aacaaaatgg tgtaataaat
     4441 acatcaggga taggaataac ccagcattaa aaaaacctct taaaacattt ggcgaaaaag
     4501 atgctgcttt aactactaga gtagcagcaa atgctaatat tgatgatcaa aaagtattga
     4561 atgactactt gcgtggacat tttttatata tgagcgctga gaacatgaac ggaagtattt
     4621 tagaggaata tttagccgaa gtgttagaac ctgaaggttg gatttggtgc gccggctctg
     4681 tatatcgagc tgttgatttc tgttatttag gaacctcacc aattctttta caagtgaaaa
     4741 ataaatacaa tacagaaagc agttcgagtt cagctataag agtaggaaca acaattagaa
     4801 aatggaatcg tttaaataag tcgacaaaaa tttctggttt ggatagccct atccccaatt
     4861 ggaaagcatt aatagaaatg actgaagcta gtaaggaact ggcagctaag ttaactgaaa
     4921 atagttattt agcttatatt aatgaaaaat ccacacgaga attatggaca ctcgatgact
     4981 aacaatatcc ttattagtat ctatccacga tacatggaaa aaattataaa caaacaaaaa
     5041 aactttgaat ttagaaattt ctatcttacc tcagatcctt ataatatctt ttggatttac
     5101 gaaacaatcc ccaccaaagc tttaaagtat aaattgattg taaaaaatcc aattactaaa
     5161 cttaagaaaa atcaacacta ctttttagga gacgatgagt ttgaacaatt agtttaaaag
//
LOCUS       DAYYFU010000115         5039 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000115 DAYYFU010000000
VERSION     DAYYFU010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.121"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            413..619
                     /locus_tag="ACHA34_002743"
     CDS             413..619
                     /locus_tag="ACHA34_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFM8793317.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(821..1822)
                     /locus_tag="ACHA34_002744"
     CDS             complement(821..1822)
                     /locus_tag="ACHA34_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8793318.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1827..3740)
                     /locus_tag="ACHA34_002745"
     CDS             complement(1827..3740)
                     /locus_tag="ACHA34_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8793319.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3908..4414)
                     /locus_tag="ACHA34_002746"
     CDS             complement(3908..4414)
                     /locus_tag="ACHA34_002746"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8793320.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4574..5014
                     /locus_tag="ACHA34_002747"
     CDS             4574..5014
                     /locus_tag="ACHA34_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8793321.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
BASE COUNT         1391 a         1008 c          769 g         1871 t
ORIGIN      
        1 tatttttgtt tcttattttc taaaaaatta aaaaaaattt tggtgaatta agtatttcct
       61 tgataacact ggcttttggc ctcattttag gttatcattt gtaattttat ttcacagttg
      121 atatgattgt tatgtaaaat aaaaaagttt aggatacatc gaatttttgg ggaaattctt
      181 ttgggggtat cctaaatctt ttctattata ttcccatatt ttctattatt ggtctagacg
      241 tatgatggta aactttgtac ctatctgatt gctgaatcat taattgctta tcattagcca
      301 tctttgtttc aagttcgcta gatgatcatt ttattcacat cctaacaaac ttgtcacatt
      361 tatagattct taatctattt atcatacaat tataaaaaag gaggtgctgc ttatgaaagc
      421 tatccatatt tctttgcttt ttttcgctag tgcattgatt ttatttgcta ttcatctttg
      481 ttcccagcct tatatcaata acaacggaat attagtggaa ccagcattct tttgcttacc
      541 actcggcttt ttgtcccttc ttgcctccgc aggcttcgga tttgttgctt tttttaaacg
      601 atcaaaacaa caaatctgac ttataaaatt tacgtaaaag agatcgtgac aataatcgtg
      661 tgatattccc aaatttcgaa taaaaggtgc cccttgccat ctctaactcc tcagtcacaa
      721 gttacaatat tcataaacca tgaaaagctt gtttattgaa attgctcctt gtgacttgga
      781 gctaagtgac agattacaac ctcttttgca tcataagact ttactccttg aaaaacatta
      841 ctaacttttg atatgtttct tgcgcttgtt tattgctgaa atcaaactga tattcatggt
      901 tcaaaacagc atttttctcc gcataatcta cttttgtgac tttcaccccc gctttctcaa
      961 atacagaagt tacttcttcc atttgtttcg taaacgtatt tgtatttcca tccgttaaga
     1021 atgtgttttg cgtccaacga tccgcatgtc tgaccaaggc taaacttttg accttctctg
     1081 attgttcaaa gtccttatta tcaaagtaag cccatccaca tttttcataa agaaagctag
     1141 agattttatc acttacttga gtaagtgcag aaggatccaa cagaccagac aaagaaataa
     1201 acttcgtaat ctgcttgttc tccaaagtcg gatcaagatg atcagctttt ttaatttctt
     1261 cattttgaag tgctaacaaa tattctccac taatttgtgc acctgccgaa tttccaccaa
     1321 tcgaaatatt cgaccaatct gcttgatctt ttatctcttt tttagctgct tgaatggctt
     1381 cgttcaattg gataactggt gtaggatgtt ttgcttccgg tgatcgttca taatctatat
     1441 ttacgatgat ttgctgcgtg tctgaacata gatattccaa ataatcacga caatcatttt
     1501 tatcccctcc tacatatgcg cctccatgca tccagaaaag aatcggtaat tttttctttt
     1561 ccactttcgg tgtgtagata tccatattat tatttttgta ctttgaagga taggatattt
     1621 ctttttttat atcgatttgt tcgatttttt gctggaaatc atctggtcgg gcatatactt
     1681 gttctttttc acttgtgtca aattgttttc taacaagcaa gctaatcggc tttggtgtga
     1741 tttgattaat caaaaataca gtgactaaaa taactacgat gataactggt actgcaatca
     1801 ctattcgctt gaaccatttc attcatttac cctctctttc ttcgccaaat ccatgtgata
     1861 atcgtgctaa cagataaaaa tccacctgaa taaatgagaa gcgaatgttt cttttctcca
     1921 gttttcggca acattctttt ttttgcatct gtcttttttt gtactttcac tgtcatctga
     1981 atcggttgcg tatcatcact tgattgatcc tctcgaggtt cattctttcc cttattgtct
     2041 gcattctctg tcggatcttg cttttttggt ggagctactg ggattttttt gtaatagaaa
     2101 ataatagttg gtatcgtctc attaaaaatc ccttcagtcg gtccttcaac atttgctaat
     2161 tcgtatcctt cgaatgtctt ttgctctgtc atataatttt ctccataagc tccgtaaagc
     2221 tggatttcgt cctggtcttt gagtagactt ttattgtttt catccacata ctttacgttt
     2281 atctgatcat ggaacactac tttcaaaaag tccgttacat gtttcgtgta ggcagcatgg
     2341 tcaacttcct gtgcctttcc atgccctgct ccttctacta ataaaagatc tttataccct
     2401 cctgataact catataattt ttgcgcgtta gacacaggta ctacatcatc tgctgttcca
     2461 tggataatca atttaggcaa tgaatctgac attccaatcg taggtagagc agcttttgta
     2521 tccaaatgca tctcctgatc aaaatagtat tgtttcaaaa ggcccataaa taaatcttca
     2581 tgctcttgat ccccaatgat gtctaactga ttttttatgg catccggtag tgcgggggct
     2641 actgtctggc tgtacgtaaa acgtagttgt tcatcaattg atgcaaatcc gcaattttca
     2701 ataattcctt tgacattgac aggaatatct ttcgctaata cagacatcat cgtagctgct
     2761 cccatcgaac caccataaag taaaatcttt tgagatggcc gctctcgaac ttcttgattt
     2821 atccagtaaa ggacgtcatc tgactcatat tgtccatagg tcacataatc gcctccgcta
     2881 ttaccagtcg ctctggaatc aacaaataat aaattatacc cggccttata aaaatcattg
     2941 tattcttctg taacgatccc gttattccaa ttccctcgaa atccgccatg gatcatgact
     3001 gttttatctg aaccttgatc gacataatat gcatatacat ccatcatttt gccatcttgt
     3061 ttgtttagta cagtttgatg aagttgtttt gtatttggat agaagcctgt gttttctgta
     3121 ggcgttgtcc catcatctgt acctccaagc atcgacatca acacactgtt ttttgcaaaa
     3181 gcgatatcgt acaatagata agtagcaatc agatgtttga taatctcttt atctacacca
     3241 taatcattaa cagcaagctc agtgactaat aactctatta attttgactt gatgtcttct
     3301 gttggtccat taagtatttc tgtcatctgt ttaccgaagt aattcaaata ttctttacca
     3361 ttgaaatttt cacctttacc gatatcatca ttttctgtcc cattctcttt gtcacttact
     3421 ttagcagaag aggattcgtc gtttgacatt tttgtagctg ttgcttcttc tgtttgttgg
     3481 ttatctgtgg tagcggtcgt ttcttgatct gtgctagtaa cagatgtctc aaccgttgtt
     3541 tcagtatttg tttctgtttg tgaagtactc gatttcgttt catttccagc atcagtagtt
     3601 tctgtggttt ctacctcccg agattccgat tcggaagaaa tgctctctcc atatactgtc
     3661 cattgcggga cgcttgtact tacagcaaat aagatgacca acgctccctt taatacttgt
     3721 tttgttctca agctattcac tcctttttat gtttctagga gaattatatg taaacgcaaa
     3781 cattttttat acaaaaaata aatggtttat cccataaaaa tagaacagga ttattttctc
     3841 attttacgta ataaagcaga atggataaac cagtatccat tctgcttttt ttaaacgaag
     3901 aaacaactta ttgctccaaa aatgtcagaa atcgtttatc ccaaattttc tcgtaaaaag
     3961 cgattgtttc ctttgctttc ataaaaggat tatcataagt tgtcgttgct cctctacaca
     4021 tcataagctg gtatcctaat ccatgagcaa acttcacagt cgtatctata caatactctg
     4081 cttgtgcccc acataattcg atcgaatgac agtccaattc agttaataaa acactcaaat
     4141 tagtcttata aaatgaattc gcatgggttt ttctcacaaa ataactgtgc tcaggaatag
     4201 ataattccga caatagctcc catggttttg tttgtggcac taattgagca tcttcgtgtt
     4261 gaacgaaaat aactggtttc ccaatcgttt cgtatgtttt gattctttta ttcactaatg
     4321 ctgtcactgt ttctaattcg tgtatcggcg aagaatcaaa acatacccct ttttgtaaat
     4381 cgatcactac tattgcgtcg gctgtgtttg acatacttgt gccttctttc ttttttcttg
     4441 atttaatagt atttcttatc tagctagttc cattatacgt gacaacattt tcttatttta
     4501 cacctctaaa tggcactttt cgactatttc ttcatgttat tatttcataa atacttagct
     4561 attggaggaa tgtatgatga ttaaattgta tacaggttct ggctgctcgt cttcaagacg
     4621 cgcaaaacgt tggcttatcg tccatgatat accgtttatc gaacagaact taattcgtca
     4681 accactaacc aaaaatgaat ttatccactt actaaaattg acaaacaacg gcgtgaccga
     4741 aatgctctct actcattcta ttagttataa aaatcttgtt cacagaatag atgaactcag
     4801 tttgagtgaa ctatatattg agattcgtac atccccttct ttattgaaaa aaccgattat
     4861 ctacgaccaa tctgcaaaac ttttgactgg atttagcgaa gataaaatca ggatgttcct
     4921 ttctaaaact aaaagaaagc aagagctcaa cgaactcttg ctttctggtc atcttgtcga
     4981 ttttaaagaa aataatcttt ttgcaatgtc ttaattttgg cataaacaat ctgcttttc
//
LOCUS       DAYYFU010000116         4855 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000116 DAYYFU010000000
VERSION     DAYYFU010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.122"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..360
                     /locus_tag="ACHA34_002748"
     CDS             <1..360
                     /locus_tag="ACHA34_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8793322.1"
                     /translation="FLTGDYSLESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVH
                     LAGLRVPQNISLIGFDDSPVVSYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQS
                     KIELSTLLILRESINYL"
     gene            420..1841
                     /locus_tag="ACHA34_002749"
     CDS             420..1841
                     /locus_tag="ACHA34_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFM8793323.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            1834..3333
                     /locus_tag="ACHA34_002750"
     CDS             1834..3333
                     /locus_tag="ACHA34_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFM8793324.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            3372..4766
                     /gene="uxaC"
                     /locus_tag="ACHA34_002751"
     CDS             3372..4766
                     /gene="uxaC"
                     /locus_tag="ACHA34_002751"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8793325.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         1565 a          913 c         1013 g         1364 t
ORIGIN      
        1 tttttaacag gagattacag cttagaaagc ggcctccaaa acatggaata cctattatca
       61 ctttctgacc tgcctactct ggttttttgc ggcaacgacg acatagcgat tggtgcattg
      121 agagctgtac atttagctgg cctccgtgtt ccccagaaca tttctctgat cgggttcgat
      181 gattctccag tcgtctccta tctcaatccg ccgcttacca ccgtacgtaa gcctttgaaa
      241 gaaatgtgtc aagaaggaac aaagctttta ctagatttga tcgaaggcag gacgatcgaa
      301 caatctaaaa ttgagctctc caccttgttg atccttcgtg agtcgatcaa ttacttgtaa
      361 actacaagta attttatata aaaatgttaa cgtgtgcata acacagaaag gaagtttata
      421 tgaaaaaact gacaaaagaa ttagtgaaaa aaaacgaagc acctgtaaaa gttcttcagt
      481 ttggtgaagg caattttatg cgtggattta ttgattggca gattcagcag ctgaataacc
      541 aacaattgtt taaaggtaat gttgcaatca ttcaaccttt agaacaaggt cttggaaata
      601 tgatgaacca gcaagataat ttatatacgg tcattttaca aggactcagc aatggcgaaa
      661 tcgttgatac ttccgagatt atcaccgtag tagaacaaat gatcaatcct tatgatgaat
      721 gggatgctta tctatctcta gcagaaaatg acgacttggc ctatatcgtc tccaatacaa
      781 cagaggcagg aatccaattc gtggagactg ataatttaac agatactccc ccacagagct
      841 tcccgggcaa actgacagcc tttttatacc gacgtttcaa attaaacaaa gctggcttta
      901 ccatcattcc ttgcgaactg attgacagaa acggcgaacg tctaaaagaa atcattttac
      961 gctacgcaga aaaatgggat ttagaacctg cttttgttga gtggttagaa aaagaaaatg
     1021 ttttctgttg tagtttagtt gatcgcatcg tcccaggtta tcctcgcgaa gatgcaggtg
     1081 ttttcaatca acggcataac tatgaagatc aattgatggt caaagcagaa ccattcatgc
     1141 tttgggtgat cgaagggcct aaagaattgg agaatcaact accattcaaa caggctggct
     1201 tgaatgtcat catcacagat gatatgactc cttatcgtga acgtaaggtg catttgctta
     1261 atggaccgca tacagcgatg gtccctcttg ctttgttggc ggaattggaa actgtagaag
     1321 aagtaatgaa agatccttta ttctccgcat atgttgatca gttattcaat ctagaattga
     1381 ttcctatgct ttctttacct aaagatgaat tagccattta tgctgaccaa atcaaagaac
     1441 gtttcttgaa tccttttgcc catcacaaat tagaagcgat ttcattaaat agtgtttcaa
     1501 agttcagcac ccgtctgctt ccagtgttca agaagtatat cgaagaacaa aatcaggtac
     1561 caccgttaat cactgtttca ctagctgcgc tacttcttat gtatcgaggc gatcaagtaa
     1621 agccacatga tgatgaaaag acgataagcg aatttactga tgcttggtct gataatggaa
     1681 cagctattcc tcgattattg caaaacgctg cgctttgggg agaagatttg tctcaaatac
     1741 cgaatgtgac agatacagtt caagaaatag ctgcaaagat cgatcaaaaa ggtgtccggt
     1801 cagtattaca agaaatgaaa ggaacgatgg tccatgcttg aaaccctgca accaacaatg
     1861 aaattaaatc gacgagacag tgtggtcgtc gcattaaccc ctattccaaa acaaacacaa
     1921 ctgacgattg atcagcaaac gatcaccact cttgaagata tcccgcaagg tcataagatt
     1981 gcattgggtg atttgaaagc tggagacaac gtgatcaaat atggctatcc aatcggtcat
     2041 gtgacgacag acgtaactgc cggtcaatgg ctgcacactc ataatgtaaa aaccaatctt
     2101 tctggtgaat tggattatcg atatgaaaaa gatgtccatc caagtcatta cctttttgag
     2161 aaccgaacat ttcaaggtta cttgcgaaaa aatggaaaag tagggattcg taatgatttg
     2221 tttttagtac ctaccgttgg gtgtgtgaat ggcattgctg aactgatcgt caaacagttc
     2281 aaggaaaaac atccagatct gggacagttt gaccatatta cgattttaaa gcatccttat
     2341 ggctgttcgc agcttggaaa agatcatcaa aatactcgag aaattttagc ggacgcagta
     2401 aatcatccca atgccggagg agttttagta tttggattag gttgtgagaa caatacagtt
     2461 cctgaattca aaaaaatttt aggtgaatat gatgaagaac gagtgaaatt tctagtggct
     2521 caagatgttt acgatgagat cgaacaaggt gtggctctat tagaagagct tttaacagcg
     2581 gcggaaaatg accagcgcat atcagtcccg ctctctaagt tgaatgtcgg tttgaaatgc
     2641 ggcggttctg atggtctatc gggaataact gctaatcctt tacttggtgc attttctgac
     2701 tacttgattg ctcaaggagg cagcacgatc ttaacagaag tgccagaaat gtttggcgca
     2761 gaacaagtat tgatggcacg agcggaaaat gaagaagttt ttgactcaat cgttgacttg
     2821 atcaatgact tcaaacagta tttcttgtct tacggggaac cagtttacga aaacccatct
     2881 cctggtaata aagcaggtgg gatcactaca ttagaagata agtctttagg ctgcacgcaa
     2941 aaatcaggaa gctctcctgt cgttgatgtg ctacaatatg gcgaaaaaat cagaaaacca
     3001 gggcttagtt tattgcaagc tcccgggaat gatcttgtag ctgcctctgc tcttgcttca
     3061 tccgattgcc agttggtctt gtttaccaca ggaagaggga ctccgtttgg ctcatatgtc
     3121 ccaacggtca aggtatctac gaatacaact ctttttgaac gcaaaggtca ttggatggat
     3181 tttaatgcag gcgtcttatt agaacaacca atggaagtaa tcttagaatc gtttgttcaa
     3241 aaaatcatcg ctgtagctag tggagaagaa acgaaaaatg aacaaaatga cgttcgagaa
     3301 attgccattt ttaaaaacgg agtaacactt tgattttcat aaaacagtat aaaaagaaag
     3361 aaggaagatt tatgttttta tctgatgatt ttttattaaa agacgattgg gcgaaaaagc
     3421 tttaccattc ctacgcaaaa aacatgccca ttattgatta tcactgtcac ttagatccaa
     3481 aagaaattta cgaaaacaac aatttcagca atctgacaga agcttggtta ggaggcgatc
     3541 attacaaatg gcgcttgatg cgagcttgcg gcgtgcctga agaaaaaatc acaggaaatg
     3601 cttctgattt tgaaaaattt ttagcttggt gtcaaaccgt accaaaaatt atcggaaatc
     3661 cgctttacag ttggtcacat cttgaattga agcgtttctt cggtattgac ttgttgataa
     3721 atgaagaaaa tgcagagaaa atttggacac aagcaaatga aattttggca gctcctgatt
     3781 ttcgccgtcg ggaaatcgca aaaaaatcga atgtagaagt gatttgtaca acagatgatc
     3841 ccgcagacga cttgcactat catcagttgc ttgctcatga agaaccagat ttgaaggttc
     3901 taccttcatt ccgtccagat aaagcactca atattgaaaa agaaggattt aatgcatggg
     3961 tcacactact ggaagaagca gctgaccaaa cgatcaccac ctatcaagaa ctgatagaag
     4021 tcttagggca gcggatagaa tacttccatc aacatggatg tcgtgtttcc gaccacgctt
     4081 tagacatctt acgctataaa gaagcggatg aaacagtatt agaagagatt tttcaaaaag
     4141 cacgagctaa caaactatta tcttcagatg aaatcgatgc ttatcttaca gaaacactga
     4201 tacatttgac ccacttctat cattctcata attggacgat gcagctgcac atccatgctt
     4261 atcgtaactg taatacacaa atgttggaaa aattagggcc cgataccggc tatgatggaa
     4321 tgaatgatct ttctttgact attcctctgc aaaaattatt gaaccgtgca gaagaaacgg
     4381 atcagttgcc aaagacgatc ctttattcat tgaatccgaa tgactatcct gcgattttag
     4441 ctttgatggg ctgtttccaa aaagaacaga atggaaaact gcagttaggt tctggatggt
     4501 ggtacaacga tactcgagct ggtatgcgcg atcaattgac acaatttgct gatggtagtg
     4561 ctttggggaa ttttgtcggc atgctgacag attcaagaag tttcctgtcc tacactagac
     4621 atgaatattt ccgtcgtgtt ctttgtgaat ttttaggcga aatggtcatg cgaggagaag
     4681 cacctgaaga tgaacaactt ttaggaagtc tagttcaagc gattagttat acaaatgcta
     4741 aaaattactt tggctttttt gcctaattga ttaatagtaa cagtaacgaa tcaaaaacag
     4801 gatagtgaaa taacggaatc cgtttatcca cgttttagag aagaagacgg accaa
//
LOCUS       DAYYFU010000117         4788 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000117 DAYYFU010000000
VERSION     DAYYFU010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.123"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            564..989
                     /gene="bacA"
                     /locus_tag="ACHA34_002752"
     CDS             564..989
                     /gene="bacA"
                     /locus_tag="ACHA34_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFM8793326.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1019..1183
                     /locus_tag="ACHA34_002753"
     CDS             1019..1183
                     /locus_tag="ACHA34_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793327.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2141..3118
                     /locus_tag="ACHA34_002754"
     CDS             2141..3118
                     /locus_tag="ACHA34_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8793328.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="ACHA34_002755"
     CDS             3128..3625
                     /locus_tag="ACHA34_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8793329.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="ACHA34_002756"
     CDS             4001..4282
                     /locus_tag="ACHA34_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793330.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="ACHA34_002757"
     CDS             4284..4700
                     /locus_tag="ACHA34_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793331.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1757 a          816 c          786 g         1429 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DAYYFU010000118         4720 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000118 DAYYFU010000000
VERSION     DAYYFU010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.124"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1097)
                     /locus_tag="ACHA34_002758"
     CDS             complement(<1..1097)
                     /locus_tag="ACHA34_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8793332.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="ACHA34_002759"
     CDS             complement(1227..2603)
                     /locus_tag="ACHA34_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8793333.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..3259)
                     /locus_tag="ACHA34_002760"
     CDS             complement(2603..3259)
                     /locus_tag="ACHA34_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8793334.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3269..4546)
                     /locus_tag="ACHA34_002761"
     CDS             complement(3269..4546)
                     /locus_tag="ACHA34_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8793335.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4565..>4720)
                     /locus_tag="ACHA34_002762"
     CDS             complement(4565..>4720)
                     /locus_tag="ACHA34_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFM8793336.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         1477 a          858 c          566 g         1819 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttctatctt tagctaatgt ctttaatata ttaattattt
     2761 ctccagcagt aacactgtct agattaccag taggctcatc agctagtatt attggagcat
     2821 ctgataccaa tgctctagca atagctactc tttgttgctg tccaccagat aatttcataa
     2881 catttctttt tatttgtttt ttatctaaac ctagttcaaa caagatactt tcatctgctg
     2941 atttatttac taatctaata ttttcaattg gtgataaata atctattaaa ttatagtttt
     3001 gaaatactaa agatatatta ttttttctat gattactata tcctttattt tgtatatctt
     3061 cattcttaaa caatattttt cctgtttgag gtttatcaag tccagcaagt aaggaaagaa
     3121 gtgtagattt tcctgttcct gactttccta ctatcgcata aaacttccca agttcaaatt
     3181 tttgatttac tcctgacaaa actttttctt tagaatttgc ataactatat gctacattct
     3241 ttatttctaa tatatccatt attttctcct aacttatttt tgataagatt tctttaggct
     3301 tcttgattaa cattaatgaa gaagcaaata caactgataa aacaataata cttattaata
     3361 ttaaataact ttgtccaagt gttgttatat ttgacataaa actactatta tttattaaac
     3421 ttccaccgga aatcattgaa tcctctgagt taatgaatcc atctacaatt acttttagta
     3481 atacatttcc taaaaataag gaagatatta tacttggtat cgatatgaat attaactcga
     3541 atataaattg cattataatt tgtatcttag atgttccaat agataagaat atacctattt
     3601 cataaattct ttcccttaac catagaatca agattaatga aagaacaacc atcccaccta
     3661 acataataga ataagtcatt acttttatta tatattttat tccactcact gactctaaag
     3721 actcttcaaa cgcattagaa tctttttcaa caaaatactt tgactcatca attttaagct
     3781 ctttcaattt gtttaaggct aagtctgtag attctgcact accagaatac attaaaattt
     3841 tatttgcaat ttcattattt tctgaattat ttaatatctc ttggcttgtt gaataatcta
     3901 caaacaccat attttcacta aaatcagaag ataatcctgt atatgtttcc tgttttttac
     3961 cagaaaagat ccctataatt ttaaatttat gactttttat ttttccactt ttttcaatat
     4021 ctagtaattc aagatcaact tcatcaccta attttagatt gttttgttta gcaaattctt
     4081 catgaacaat aattgaattc ttatcatttt cttctatatt tttaccttct ttaattgtaa
     4141 atactccact actaaataaa atatttcttt tagtattatt tgtagcttcg agtgaaacaa
     4201 cattcttaaa ttcgtcagat aaatcttctc tatttattct ttgttctccg ctaactactt
     4261 tagcatcttt tagttttgct aatccatcat attgaattat tttttcttca atttctttta
     4321 atttttcaat atctttaaat tgattaacat taaaatattt accatctttt tttgttattg
     4381 atattgaaga attagaactt tcatataaag ccttttctat ttcattgctt gatttcatta
     4441 ttgttaaaca agaatacaag caagaaagaa ctatagttaa tatgataaaa attattaaag
     4501 ttctattttt ctttcttgta atatatgcta tagcattttt tatcactttc ctaactactc
     4561 ctctctattt tcttactcca taactaattg catccactgg acaagcattt ttacatcgtc
     4621 cacatctgat acactccaga tgattgcaat tctcaactga atctttattc atttggcaaa
     4681 cttttttaca ttttccacaa ttaatacact tatccttatc
//
LOCUS       DAYYFU010000119         4711 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000119 DAYYFU010000000
VERSION     DAYYFU010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.125"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            106..357
                     /locus_tag="ACHA34_002763"
                     /pseudo
     CDS             106..357
                     /locus_tag="ACHA34_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="ACHA34_002764"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="ACHA34_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFM8793337.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1737
                     /locus_tag="ACHA34_002765"
                     /pseudo
     CDS             1654..1737
                     /locus_tag="ACHA34_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            1786..1869
                     /locus_tag="ACHA34_002766"
     CDS             1786..1869
                     /locus_tag="ACHA34_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFM8793338.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="ACHA34_002767"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="ACHA34_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8793339.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="ACHA34_002768"
     CDS             complement(2994..3857)
                     /locus_tag="ACHA34_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8793340.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="ACHA34_002769"
     CDS             complement(3859..4131)
                     /locus_tag="ACHA34_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8793341.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4363)
                     /locus_tag="ACHA34_002770"
     CDS             complement(4148..4363)
                     /locus_tag="ACHA34_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM8793342.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4711)
                     /locus_tag="ACHA34_002771"
     CDS             complement(4455..>4711)
                     /locus_tag="ACHA34_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HFM8793343.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1446 a          796 c          865 g         1604 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc a
//
LOCUS       DAYYFU010000120         4646 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000120 DAYYFU010000000
VERSION     DAYYFU010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.126"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..717)
                     /locus_tag="ACHA34_002772"
     CDS             complement(133..717)
                     /locus_tag="ACHA34_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8793344.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(928..1131)
                     /locus_tag="ACHA34_002773"
     CDS             complement(928..1131)
                     /locus_tag="ACHA34_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793345.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1141..1563)
                     /locus_tag="ACHA34_002774"
     CDS             complement(1141..1563)
                     /locus_tag="ACHA34_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793346.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1801..2415
                     /locus_tag="ACHA34_002775"
     CDS             1801..2415
                     /locus_tag="ACHA34_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8793347.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2580..3404)
                     /locus_tag="ACHA34_002776"
     CDS             complement(2580..3404)
                     /locus_tag="ACHA34_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793348.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(3428..3583)
                     /locus_tag="ACHA34_002777"
     CDS             complement(3428..3583)
                     /locus_tag="ACHA34_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793349.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
     gene            complement(4042..4299)
                     /locus_tag="ACHA34_002778"
     CDS             complement(4042..4299)
                     /locus_tag="ACHA34_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8793350.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(4292..4561)
                     /locus_tag="ACHA34_002779"
     CDS             complement(4292..4561)
                     /locus_tag="ACHA34_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8793351.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT         1533 a          816 c          626 g         1671 t
ORIGIN      
        1 taatattaac aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact
       61 tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat tttcagcatt
      121 aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt ttgccaaata
      181 acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa taggtgtacc
      241 gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt caggacgacc
      301 accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt ccaggatcaa
      361 attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc ctgtagaact
      421 tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac gctcattcag
      481 tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta agcgccaaac
      541 gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg gtcgattact
      601 ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat ttaggctgtc
      661 ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg gcgtatccta ttttcaaaaa
      721 aatcactcct tcattaattt tattgtaaca taactttatc catatagttt aactgaacat
      781 agatattgtt atgggtttat gaacatgaaa tgaattgttt ttttgaaaaa taaagcaaaa
      841 aaagaaagtg tccagaaatg ggtgatttta tgaacacttt caattagaaa aaaagaatgt
      901 cgactagaca ctcttttttt aaaatcatta tttttctttt tgatatgttt tttggtctct
      961 tttttttctt ttttcttcag aatcttctag attagaaatt gatttttgaa ttttttgtgt
     1021 ctcctgtaaa gttcgctgaa cttgtaaagc tgattcttct ttcggtaaat taattttact
     1081 tagaatatcg ctatcttgat aaaaatttgg tgtgttcaca aattctggca taaaaaatcc
     1141 ctactttcta gttaaggttc ctgaataagt cgcaatccaa ttgtttccag ctgatttata
     1201 actttgtaaa tacaattttc catagtacca ataaccattt cttttttctt tccaattccg
     1261 tgattgagga ggtctagaag gccaaccaac tttatcaaaa atcatcgttc ctgtcactcg
     1321 tatcgtttta gaagctgcta gagcaagtaa tggagaaact attaaataat tttcagtaac
     1381 agttaagtcg ttagttgggt taccaatggc tattccgtcc ctatctaaag gagttaattc
     1441 ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg taggaattgt
     1501 agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa acttattttt
     1561 cattgtctat cacctcgaaa tcaaaactaa gaaaacgttt tcttaataaa tcttaacaga
     1621 taaatcaagt tatatactag aaaattcata aaatatattt ttaatgtcat aatcgtttaa
     1681 attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacata
     1741 tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta ggtaaaagtt
     1801 ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg ccagattgaa
     1861 gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg cgcaacgatt
     1921 actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga tatttttatt
     1981 gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc agtcaattac
     2041 ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc tgaagcaatt
     2101 ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt ggccatgatc
     2161 gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa gatcgcaaaa
     2221 gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa agatccccaa
     2281 cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc gattgctaag
     2341 attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa agacagcatg
     2401 gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta atttcctaca
     2461 tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata aaattccaac
     2521 aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac ttatttacgt
     2581 taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc cttattatct
     2641 tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact aattcctata
     2701 gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat ttgagattta
     2761 tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata cataaagcta
     2821 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     2881 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     2941 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     3001 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     3061 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     3121 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     3181 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     3241 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     3301 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     3361 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     3421 aaatcagcta atttttcttt agaataaaaa aaacatttat atcagaatca ataaattcta
     3481 ttttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
     3541 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
     3601 ataaaaatta cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta
     3661 aattctatga gaagtttatt tttgttgttt ttgctaatta actaaaacta gtacaaaaaa
     3721 tttaaaatag attattaaac ctattgctta ttctattcag tcatatatat aagcgtcatc
     3781 ctttagtaac taacatccca cactacaata gtattataac atatctattc aacttcaaat
     3841 tgttgaaatt taagccattt atatttactt ttcctcaact ttagaaatga ttttactaac
     3901 cttatatccg taatacgcgt aacaaacaca ttcattaata acaatcaaca ctaagctata
     3961 ttagcttttt aaatgactag ggtaaataaa ataaggatca aaaactttta agtttctgac
     4021 cctttcctta cttccgattg gttaatagtg atcttttgca gaataaataa atatcgtttc
     4081 attttcaact ctatatatca gtctatgttc atctgtaatt cttctggacc attttccaga
     4141 taaatcatgc tttaatggct caggttttcc taatccagca aagggggaac gatcgatatc
     4201 ttttattaac ttgttaatct tttttatatt gcttttgttt ccttgctcat gccaataaag
     4261 ataatcatcc caagcatcat cagaccaagc cttaatcatc agattcaacc tcgattaagt
     4321 catgtgtttt aaatgcacct ttggagaatt gttcatctcc tcgacgaatt ttttccatga
     4381 cgtaattatt agaaagtgtt ctcaacgttt cttgcataga atcataatct ctttttgata
     4441 atacaacaac tgtatcttct acatctttac ttgttacaat aagtgtttca gcatcctcat
     4501 taacttgttt catataacta cgtaaatttt ggcggaaatt tgaataagct actgcttcca
     4561 ttcctttcac cccttattta tttctataaa acaattgtac attatattgt acaattaagc
     4621 aaatgattaa tattctaact tcttat
//
LOCUS       DAYYFU010000121         4635 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000121 DAYYFU010000000
VERSION     DAYYFU010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.127"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..519
                     /locus_tag="ACHA34_002780"
     CDS             175..519
                     /locus_tag="ACHA34_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFM8793352.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            520..939
                     /locus_tag="ACHA34_002781"
     CDS             520..939
                     /locus_tag="ACHA34_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFM8793353.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1031..1381)
                     /locus_tag="ACHA34_002782"
     CDS             complement(1031..1381)
                     /locus_tag="ACHA34_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793354.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1347..2677)
                     /locus_tag="ACHA34_002783"
                     /pseudo
     CDS             complement(1347..2677)
                     /locus_tag="ACHA34_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
     gene            2933..3499
                     /locus_tag="ACHA34_002784"
     CDS             2933..3499
                     /locus_tag="ACHA34_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8793355.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3644..3731
                     /locus_tag="ACHA34_002785"
     tRNA            3644..3731
                     /locus_tag="ACHA34_002785"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3676..3678,aa:Ser,seq:cga)
     gene            4065..>4635
                     /locus_tag="ACHA34_002786"
     CDS             4065..>4635
                     /locus_tag="ACHA34_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFM8793356.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1685 a          778 c          891 g         1281 t
ORIGIN      
        1 aaaataaaaa aagcgttagc aactatgctt aaaaagcata tcgactgaat aaactaagca
       61 ttggacaggc tgaaagacta catttaaaat gaaatgcgaa gaaacaatag aaaataaagt
      121 tgccttagaa ccatagttga atcacctaac agtcgtgaag ggagaataag agaaatggca
      181 gaaaaacaaa ccgcaggacg agatcaatta ggagattttg caccaaaatt tgcagaattg
      241 aatgatgatg ttttatttgg acaagtatgg tccagagaag aattatctgc tcatcagcgt
      301 agtatcatca ccatatctgc attaatttca ggaggaaatt ttgaacaatt gccagctcat
      361 ctgaagattg gcaagcaaaa tggcattact agtgaagaaa tagcagaaat cattacccat
      421 ttagcttttt atgtaggatg gccaaaagct tggtcagctt ttagtttagc aaaagaaatc
      481 tatttagaag aaaatagcga agataagaaa gaggcgtaaa tgacgacaaa acacgaagaa
      541 atacacaatg gcgtgatttt tccagcaggt gaaaaaaacg aagcttacgc tcaagtattt
      601 accgggcaaa gctatctgaa tatgttagtg aatgatcccg aagtttctgt tggtgtaggt
      661 aatgtaattt ttgaaccagg ttgtcgcaat cactggcatg tccataaaga cggctatcaa
      721 atcttacttg tgaccggagg agaaggctgg tatcaggaag aaggaaagcc agcaagacga
      781 ttaatggctg gagatgtcgt cgtaacgcac aaagggatta aacattggca tggtgcgaca
      841 aaagacagtt ggtttgaaca tttggcgatc actgctggta attcagagtt tttagaacct
      901 gtaacagatg aagtgtatga aggattggaa aatcaataat gtcaagcttg aacagctcct
      961 gaaagtagat tttaatcgaa ctttcaggag ctgctttttt gattcagtct tttataaata
     1021 gaaaataggg ttaataatag gaatgagaag cacgtcgaaa gatcttaata ttgataatcg
     1081 gctgggcata catgatttcc acgtcatcaa tcaaactacc taacagatga taatcttccc
     1141 gatcatgtcg gggattacct aatagttctt gatagtagat ttccatacta gggtcccgag
     1201 gattgttcag caaatcagca agtacatcta aatcttctgg ctgagtttct gccgtaccct
     1261 gtatctctat gaaaagccct tcctcagcaa attctactcc aatacgtatt tcagttattt
     1321 catgggaata taaataagta agaagctcat cgatgattct ttttgataat tctttttcca
     1381 agtgtctttt tttcctttct gacagcggta aagttatcta gtatcggtac aaggatacct
     1441 gcaacgaaac cagttgaaaa accactgtta tatagattta gtcctccgtg caaagtgctg
     1501 acgttatgga caagagcaat atgtagtaca ccagctatta caccgtaaaa actgccgtaa
     1561 aaaccgctta tgggcgcgag acctgtccca aacacagctg caacaagcat gccagtactg
     1621 gtgatctcat gaaaagttcc aaacaaagaa gcaataaata ttccgactag tatcggaaaa
     1681 ctgtttttta aatgacaacc aaaagcactg aaacccaccg cagataaaat agcaccgatg
     1741 acaggtccat ttaactgacc gccaaccagc aaaacataag aagacagcat cagaccaact
     1801 aagcctatgt tgatcaaagt agcaccaata ccagaattag caatgaaatc agttgtcaat
     1861 tttccagatg aatcgaaaat ttctcgaatc cccaaaagcc gaaaggaatt ataatagaaa
     1921 ccaattccga ctgtcagcga aaaaaagcca aaaataatcc aaatgagagg aaagtgatat
     1981 tcattgaaaa ccaatgtatt ttcgacgatt gaatagctga ataacctcaa aacagcagta
     2041 aaagtcatcg caatcaaccc agaggtgaat ccaatattat aaagtgtgaa tccacgatgg
     2101 aaaactaaag tttgagcagc taagggcgga agaagtagac caattgctat gccagctaaa
     2161 ttccccaata aaatacctat cagtaaagga aaacgcatac caaaagtgat ataactaacg
     2221 accggtgata atgcgctacc aaacagtccg atcatgatgt actgagaaaa aggtttattg
     2281 acaaatacag cgtatagata cacacctaaa atgattgaga tagaattata aaggtttttg
     2341 ccaaaaaaag agaaaccggt aactgttaga atcgaagcaa ataagggacc gtttaaaatc
     2401 accttgtgcc gataagctaa aagcacagaa agtaaggtca aaatccctac atttatgaat
     2461 gcgcttccga aaccgcctaa agcaaaataa tctgtcaaaa gattgcttgg cgaggtaaga
     2521 atagttagca atccactcca attatcagta ggagtattag aaagcaagcc aataaaaaga
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc
     2701 tttaactata acttgaaatg aaagatttgg acaactatca tttaggtcaa aactaaaaat
     2761 gtaagggaac ccattgaatt taaccaaatt tttttaattt tgcaaaaaaa agttaaagaa
     2821 gactgaaaaa aaatttaaaa tataacgttt tgagtaaaat gtttgcaaaa aatgctaaaa
     2881 atagggtaca ataaaacaac atatactgca gtaaagaagt gggggagttt taatgagaag
     2941 aaaagtctat accaaggatc aaattctaaa agctgcttat gatgttgttg caaaggaagg
     3001 gtttaaagga tttactgcga gaaatattgc taaaaagatg gggatttcga cacagccgat
     3061 ttatctggaa ttcaaaaaca tggaagattt aaaaaatacg ttgctggatt ctatctttga
     3121 acaactaaaa gaagatgttt tccctattga acgtactggt gataaaatca ttgatctcgg
     3181 tttgaactat atctactttg caaaaaagca tcatagtctt tacatggcat tgtacattga
     3241 tgaacatggg gacggaaaga aaatgcatga tttttcatac aattatttcc gtgaaatagt
     3301 taaagaagat gaaagataca atcaattatc ggatgaatat gtcgatgctc tgcatactgg
     3361 tacatgggta gcagtgactg gcttagcgtc attgatgtca tcaaacatca tgcatccaac
     3421 agaagaacaa atcattgcac tgatcaaacg gacaattgac tcgattttag cattagataa
     3481 tcctgaaata atcagatagt gttctatcag tatgaatttg atcggtaagt ccgttggctg
     3541 caaactgtca atggactttt ttgctaaaaa aagcgatttc tgttatttat ctattagaaa
     3601 taggcttgac atttcgtgaa aaaagtttaa aaatagtact tacggaaggt tggcagagtg
     3661 gtaatgcacc ggactcgaaa tccggcgaac cggctaatac cggcgcgcag gttcaaatcc
     3721 tgtaccttcc ttatatcaac gattagaggt tgtgaagaag ctgtcctgca tcaagtaata
     3781 agaacggcca aacaaaaata gcttttccta ttttttcgtt tgattgggct tattaccgca
     3841 gatgcaagct tttgaacacc gtttgttcgg aataaggggc tgtgacaaga cttttgtcac
     3901 agccccttat tgttgtactt ttaacctaca atattttttg attccccctg agtactattt
     3961 tttataaatg ctagtaaatc aatcttcttt ggcctttttt tgataaaaaa aatactagat
     4021 gtaagataaa agtgtaaaaa cgatttgttt taggagggga aacgatgtgt accgcaatca
     4081 cttatgcaac gaaagaccat tattttggaa ggaattttga ttatgagatg tcttataatg
     4141 aagtggtcac tattactcca agaaattatc gtttcgattt tagaaaggta aaaaatttag
     4201 ataaacatta tgcaatgata ggaatagcag ctggggtatc caactatcca ctctattatg
     4261 aggctacaaa tgaaaaaggg ttgagtatgg caggactgaa ttttcctggc aatgctgatt
     4321 ataaagaatt acaagaagga aaagataacg ttgcaccatt tgagtttatt ccatggatac
     4381 tagggcaatg ctcaaccata gacgaagcta aagaattatt ggctactatc aatctagtca
     4441 atatcgattt tagtgaaaaa ctacccttgt cacctttaca ctggttatta gctgataaag
     4501 aaaaatcgat agtaattgaa agtatgaaag atggacttca tttatatgat aatcctgtcg
     4561 gtgtgttgac aaataatccg ccatttgatt atcaattatt taatctgaac aattatcgct
     4621 cgctatcaaa tggaa
//
LOCUS       DAYYFU010000122         4609 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000122 DAYYFU010000000
VERSION     DAYYFU010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.128"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(84..398)
                     /locus_tag="ACHA34_002787"
     CDS             complement(84..398)
                     /locus_tag="ACHA34_002787"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8793357.1"
                     /translation="MLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQ
                     RYGATKIHQVLLKEGISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQ
                     KN"
     gene            complement(449..703)
                     /locus_tag="ACHA34_002788"
     CDS             complement(449..703)
                     /locus_tag="ACHA34_002788"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8793358.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(896..1171)
                     /gene="prgO"
                     /locus_tag="ACHA34_002789"
     CDS             complement(896..1171)
                     /gene="prgO"
                     /locus_tag="ACHA34_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFM8793359.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1143..2096)
                     /locus_tag="ACHA34_002790"
     CDS             complement(1143..2096)
                     /locus_tag="ACHA34_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8793360.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2708..4201
                     /locus_tag="ACHA34_002791"
     CDS             2708..4201
                     /locus_tag="ACHA34_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFM8793361.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4336..>4609
                     /locus_tag="ACHA34_002792"
     CDS             4336..>4609
                     /locus_tag="ACHA34_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM8793362.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1541 t
ORIGIN      
        1 gcttgaattc ttgtgaaaaa gttcttcgtg ttctcttaga cataaaaatt cctcctggta
       61 tgttttcttc tagtctacac accttaattt ttctgtctag ttaattgtag cctatccatg
      121 atcggtttat tagacctttg aggtctatat tttttaacta caatcgacct taaatttaat
      181 tgtttcatta gcttttggac atgtttaaga gatactgaaa tcccttcttt taataaaact
      241 tgatggatct ttgtggcacc atagcgttgt ttattcgtaa aaaaaattgt tgaaatcttc
      301 tttttcaact tattatttct gatttctgta gctgtcaacg gtttattctt ataaaaataa
      361 tagacacttc tagggatttc taataaccga cacaacaaag aaacagtgta atccttacac
      421 catttttcaa taaattgaac aagcgaattt aggttttccg agagaatatg gctgcggctt
      481 tttttaagat ttcaagttcc tcatttaaac gagcattttc ttttctcagt tcagctactt
      541 cttttccagt cagtcctgtg gactgatcag gtaaatataa attcttccat ttgtaaatcg
      601 ttgcttcaga taagccatat tctttcgcta accctcgaac agaacgtcca gtttgattca
      661 gttcaacaat catttgttta aaattttctt catatcgggt cataaaaatt catcctctcg
      721 tagataagtt taatttattc ttgtctctac gaaaagctgt ctaagatttg atactaacac
      781 caaatgttgt atacccccaa acaatacaca tattttgaag gattaggatt aataaagcta
      841 aaaatatagt aagtatacta aatatattta ctatatctag tatacttact atattttaat
      901 cttttgatcg tctattatat ataatccgtt cctcgtcgtt taaagtatca acataggcgt
      961 ccattgcaag tttgactatt tcatacatag gaatatcttt tgcataacta agatcccgaa
     1021 tggtttttag tacgggagga tctacttgaa ctggttttct ttctttcgca gtgtactttc
     1081 gttcccctaa ctcgacatta atatcatctt taattgaaat atcttcagca ccaattttct
     1141 tcttatcttt gttcgttggt aataatgcca tttagtttaa tctcctttcc aagaggtagg
     1201 atatgattgg ccattgagtc agcatattta ggtgtataat caaatccttc aatatcacca
     1261 tgttgttcat agtattcaat tctttcttct aattctgtaa agatgtctgc aaaaatagcc
     1321 caccatcttc gatctacata atcatttaat tggataccat tctcaccata catttctaac
     1381 cgatccgagc ctttaacaat gcttttaaaa atgtttgatt caccatactt atcaattgtt
     1441 ttttgatagt ttgcgtgttg agaggaacgt cgcactgaga aaagaacagg caaaattcca
     1501 atgatttgaa gatgagactc ttcaaaattc gtgacaatcg gaactaaaac tttattaata
     1561 aaatcttctg ttccttccat tgcgtaccgt tttaattctt gcattgcaat aatataatct
     1621 gcagcagtta agaatgcacg aaccacatta tctgttgatg gtgggcagtc gataaagata
     1681 taatcgaaat tttttctcaa ttcttctaaa ggtttaatga aggccaatga tctcttacga
     1741 cgatcaggat atttttcgga caagtattca ttaagttctt cagatccagg agagccagcc
     1801 ataatatata aatgtggact tacttgagta atacctgaaa cccagtcccc ttttttgatt
     1861 gctgcagaca tggatcgatc aaagctagta atttgatacg ttgatccaat gttggaagta
     1921 gtatttcttt gttggtcata atcaattagc aacacgtctt tttcaaaaag ttgactagaa
     1981 acaatcgcta ataaatcatt gtctgtagtc ttaccaatgc cgccttttct acctgtaagc
     2041 attacaactt ttcctagttt atctgatgtt ttaaatgcag tccgtaagtt ttccatagtt
     2101 ccctccaatt tcctatacta aatatattta gtatacttca tatacttaat atattcgata
     2161 tatctaatat actccaaatt gataagaatt cagactttat aaggaatata gtggatatac
     2221 taaatatatt tgctatattc agtatatcag atatattcaa aatgtcaaaa tatatttgtg
     2281 cgttttgcgt tgacaattta gagtgaaaag aggtaaactt aattctatag aaaccacaga
     2341 taattcacta tttacaaagt agcaaaagca gtggtactct ttaaacagaa aaattacata
     2401 aaaaaaagag acggaaataa ttgattttag cagaatcaat tatttctgaa tcacatggtt
     2461 aggacatgtg ttcacgctcg tctcaagaat atgtaagtag ctaccaacta cttatattaa
     2521 aagttgatcg gatacctttt ccgattgcct ttattctacc aaaaaaaggc gagcaactca
     2581 acagaagaac gtttatttag tgtacttaaa ttaaatattc ttaaacactg aagagagaac
     2641 acatactatg gctgagatta aagctaaact gtgttctctt tttgtttgag aaaggacgtt
     2701 acaatcgatg ttgagcgaaa tttattcagc tattcttaaa aacggcttac gtacatacaa
     2761 atatcgaaac agcaccatga aacctgcagg tcatattgat tccagtagga gtggtgctat
     2821 ctttgcgtac cgtagcaagc aattaatgaa tgttgggcgt ggtatggtca ttacttcgga
     2881 agaagctatt ttagagaacg aaaagaaact aacgcattgg acacctaata cctatcgctt
     2941 tggtacttat gcggatgatt atcgaacagt ggttaaaggt cattctgaga aaaacttaag
     3001 tcaaatcaac acatttgtag ttgatatcga ttcaaaagag aaccatcaag gcgaaattat
     3061 tttagcctgt ttagatcaag tgggctacat gccaacgcta attttagaat cagatcatgg
     3121 ttatcaagtt tattttgtac taaagacacc aggctatgtc acaaagaaaa gcaatttcaa
     3181 ggtgatcgat gtagcaaaaa aaatttcaaa aacaattcgt ctacagttgg ctgaaaaatt
     3241 gtccggagtg gatctaggat gtaaccactt cggaattgct cgattcccac aagaaaataa
     3301 tattgttttt aaagaaatga actatcagta ttcttttgct gactggattc aatggtcgat
     3361 gaagcttgaa tcagatcaaa aaacaaaaga agatcgagaa cgaaaactaa ttgtctttcc
     3421 tgagaaaaaa gaaattcgtc aagtagatga accgtggttc gatatgttac ttcataaagc
     3481 aaacatcgtc ggcggagaag gacgtcttgg tcgaaataat gtgattttca cgctttcttt
     3541 agcttattac tcttcaggtt atgcattgga tacttgcgaa tacaatatgt tcacattcaa
     3601 cgaacggtta caggaaccgc tatctgaagc agaattagta aagattgtac gtagtgctta
     3661 ttctggtaat tatcaagcag caagcagaga attcattatt gaattgtgcc agcaatgggt
     3721 ttcaccaacc ttaaaaaata atgatctgtt cattcaacga cgtggctggt ggaaatttaa
     3781 aaagccacga gatcaaagaa aatatagtca taaaaatgaa tggaaagaag atctattggc
     3841 ttatctaagc gaaaaaagtc ttgctagtga gccttatttg aacctgtcaa aaaaagaatt
     3901 ggcttcacaa ctaacgatgc ccaagcgaac acttgataag atcttaaatg agttacgaga
     3961 ggagaataaa gtcttctaca aggtaacacg aggaagaaat ggcggaatga tcattgcttc
     4021 agtcaaaacg ttgttcgcga gggttattca actaagtaaa gaaaagagac tagattattc
     4081 aacaaggatc acagaggtat ttggactcaa cgagagctta gtagaagaga catttagaca
     4141 attatcaacc cccttgaacc ctcttaaaga agtgaatctt tttgaagtag atacaggttg
     4201 aaaaataaaa ggtgcaaatt gccttataca atatatctat gatacttctg taggtgttgc
     4261 tccctttgcc tagctacgtg taggtggtat acttataact gcgtatgcag tcttatgtat
     4321 catggaggaa gttgtatgtc gttaaaaaat tatgtctatt cacatcctgt taatgtgttt
     4381 atcattggtc gtttgggatt atctgtggaa gtgttctgtg agctttatgg ttttaagcag
     4441 gggactttgt cttcttgggt taccagggaa aaaacagttg cttctttgcc gattgagttt
     4501 cttcatgggt tgtccttagc ttctggggag actatggatc aggtatatga ttgtttgtgt
     4561 gtgttagagc aggagtatat tgagtataaa atagctaacg agttaagaa
//
LOCUS       DAYYFU010000123         4475 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000123 DAYYFU010000000
VERSION     DAYYFU010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.129"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            326..1054
                     /locus_tag="ACHA34_002793"
     CDS             326..1054
                     /locus_tag="ACHA34_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8793363.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1199..3226
                     /locus_tag="ACHA34_002794"
     CDS             1199..3226
                     /locus_tag="ACHA34_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFM8793364.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3334..4431
                     /locus_tag="ACHA34_002795"
     CDS             3334..4431
                     /locus_tag="ACHA34_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8793365.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1574 a          717 c          923 g         1261 t
ORIGIN      
        1 aaaaaatata atatacattt tttatttaat tataattaat attgattgaa gtctaggcag
       61 tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag
      121 ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct
      181 gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt
      241 cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt
      301 aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat
      361 gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga
      421 atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt
      481 tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg
      541 aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat
      601 ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa
      661 accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt
      721 ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa
      781 atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa
      841 ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg
      901 aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc
      961 ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt
     1021 agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata
     1081 aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa
     1141 atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt
     1201 gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat
     1261 catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag
     1321 caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga
     1381 ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga
     1441 caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat
     1501 tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa
     1561 tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa
     1621 taagaagatt agtttactct cggattttac atttaaatct tatttatact taggaaatca
     1681 acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag
     1741 taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc
     1801 tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg
     1861 ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc
     1921 ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt
     1981 agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga
     2041 gcaaagaagt cttacttatg atttggaagg tgtcggtcac tcttctcgga ttatacctga
     2101 tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac
     2161 tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc
     2221 tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa
     2281 gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca
     2341 gactagtatc gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa
     2401 tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga
     2461 ttattttatt gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat
     2521 tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca
     2581 atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta
     2641 taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg
     2701 agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc
     2761 gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg
     2821 caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag
     2881 tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc
     2941 ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga
     3001 gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc
     3061 tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc
     3121 tacagctcat cgtggaatca gaatagatat tcctgtagaa aaccaaagaa tcggaacctt
     3181 tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa
     3241 tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca
     3301 gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga
     3361 ccaaaacaac aattatttat aacactccta gttattttag gggcactttt taacaatata
     3421 tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc
     3481 gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa
     3541 gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca
     3601 accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc
     3661 ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa
     3721 agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg
     3781 gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat
     3841 caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca
     3901 ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca
     3961 agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt
     4021 attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa
     4081 ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg
     4141 ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga
     4201 aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa
     4261 aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt
     4321 aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta
     4381 ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat
     4441 aacaaatagt aaatttttat tattgtagga tggtt
//
LOCUS       DAYYFU010000124         4433 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000124 DAYYFU010000000
VERSION     DAYYFU010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.130"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(543..2048)
                     /locus_tag="ACHA34_002796"
     CDS             complement(543..2048)
                     /locus_tag="ACHA34_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8793366.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="ACHA34_002797"
     CDS             complement(2045..2731)
                     /locus_tag="ACHA34_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8793367.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACHA34_002798"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACHA34_002798"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFM8793368.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DAYYFU010000125         4416 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000125 DAYYFU010000000
VERSION     DAYYFU010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.131"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..913
                     /locus_tag="ACHA34_002799"
     CDS             59..913
                     /locus_tag="ACHA34_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8793369.1"
                     /translation="MENSLYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="ACHA34_002800"
     CDS             1010..3259
                     /locus_tag="ACHA34_002800"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8793370.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..>4416
                     /locus_tag="ACHA34_002801"
     CDS             3262..>4416
                     /locus_tag="ACHA34_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8793371.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1556 a          626 c          805 g         1429 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct ctatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga tatcggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acaggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actaga
//
LOCUS       DAYYFU010000126         4349 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000126 DAYYFU010000000
VERSION     DAYYFU010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.132"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(387..1853)
                     /locus_tag="ACHA34_002802"
     CDS             complement(387..1853)
                     /locus_tag="ACHA34_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8793372.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2145..4229)
                     /locus_tag="ACHA34_002803"
     CDS             complement(2145..4229)
                     /locus_tag="ACHA34_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8793373.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1310 a          927 c          697 g         1415 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaaataa gttattttct tttatttcat tgaccttatt gaatttcaag
     2041 ctattttgaa ttttctgcaa aattaaaaac attagttatt ttataatcct atctattttc
     2101 tttacatttt tcattaagaa aaggatataa aaaaaagagg acgttcattc gtcctctttt
     2161 tttacatgca agcttcgcat ggcattcaaa acagctaata ctgtcacccc aacatctgca
     2221 aatactgcat ctcccattga tgcaaaacca aaagcaccta aggcaagtac caaaattttt
     2281 atcccaatag caaagacaat gttttgtttg acgattttca atgttttctt tgctaaatgg
     2341 attgcttctg caatcttcga tggttgatca ttcatgatga ctacatctgc tgcttcaatt
     2401 gcagcatcgc ttcccaatcc acccattgcg attcctacat ctgctcttgc tagcaccggt
     2461 gcatcgttga ttccatctcc gacaaatgcg gtctttttat tgtttctttg taagatttcc
     2521 tctaaccgct gcactttatc ttcaggaagc aattcgctat atatcttgtc cacgcctact
     2581 tgcttcccga tatgatcagc aatttttttc gagtcgcccg tcaacataac tgtgttcttc
     2641 acaccagcct gttttaattg aaccaatgcg tctacggcat cttctttcaa ggtatctgca
     2701 ataacaagat aaccactgta actttgatcg atcgctagat aaagcaatgt accgatttct
     2761 tgacttaaag gaaaagagat ttcaaatctt tccatcaatt tgtgattacc tgctaatact
     2821 tgtttgccat caacttcagc taagatccct tcacctgcaa attcctggat attagttgtt
     2881 ggtaaaagcg gacctgcgta gccttctaaa acagattgag caatcggatg tgtagaaaac
     2941 tgttctgcac tcgcaatata ttgcatgaat ttctttggat cgatttttgt atcgatcgtc
     3001 tggatcgaaa agtcaccttt cgttaatgtg ccggttttat cgaataccac tgtctcggtt
     3061 tgcgcaagca actccaagta atttgaacct tttactaata caccgatttt acttgcgcct
     3121 ccgataccgc caaagaaact aagtggcacg gaaatcacta atgcacatgg acatgagatc
     3181 actaaaaatg tcaatgctcg atagatccat tcagaaaacg ccgctccaga aacaacaagt
     3241 ggaggtaaaa cagctaataa aacggccaat cctacaacga ctggtgtgta atagcgcgcg
     3301 aatttagtaa tgaaattttc agctggtgct tttttactgc tggcattttc tactaattct
     3361 aataatttgc tgatagtaga attttcataa gatttttcta cttgaactaa aaatggttta
     3421 tttttattga taaatccgct taatactgtt tcaccaacat atacagattt agggactgat
     3481 tctcctgtaa gtgctgacgt atcgactaat gattctcctt cgatgatcgt tccatctaac
     3541 ggtacacgtt caccaggctt cacaagtaca aactgaccga tctgcacgtc ttcagggttc
     3601 acggtcatca atccttccgt cgtctgcacg tgagctgttt ctggacggat cgccatcaat
     3661 tcgcgaatcg attttcttga atgactgact gccaatcctt ggaaatactc gccgatttga
     3721 taaaatagca tcactgctac ggcttctgga tattcaccga tcaacatagc gccaatcgta
     3781 gcaacagaca taagaaaatt ctcatcaaag atttttcctt tgacgatatt catcactgcc
     3841 ttcttcacta catcatatcc tatcagcaga taaatcaata aataccctat caagcgaatt
     3901 ggttcattaa aatcaaataa tagcaataaa gcgatacctg caagggaaaa taaaatccgg
     3961 aacagcgtat ttctctcttt ttcatttcca tgttcagata ctttttcctt atctatttct
     4021 gttactttga catctggttc taattccttc actacttttt ttgtttcttg ttcaatcgta
     4081 gccaaatgat cttcctctgc gtcaatcgtt agttttccag aagtgaagtt taccgttgct
     4141 tccttgactc cattgatctt ctgaactcct ttttcgattt tcattgcaca gttcgcacaa
     4201 tctaaacctt ctaatctgta acttttcatt ttaatgacct cctcgcctca acatatgaac
     4261 atctcttcat aactaattat aaatgaatgg ttattcatat gtcaaggtat ccgaaagaaa
     4321 aaatgaaaaa catctgcatc ggaaaattt
//
LOCUS       DAYYFU010000127         4324 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000127 DAYYFU010000000
VERSION     DAYYFU010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.133"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..281)
                     /locus_tag="ACHA34_002804"
     CDS             complement(<1..281)
                     /locus_tag="ACHA34_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM8793374.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEEN"
     gene            complement(730..2138)
                     /locus_tag="ACHA34_002805"
                     /pseudo
     CDS             complement(730..2138)
                     /locus_tag="ACHA34_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(2359..2721)
                     /locus_tag="ACHA34_002806"
     CDS             complement(2359..2721)
                     /locus_tag="ACHA34_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793375.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2725..3033)
                     /locus_tag="ACHA34_002807"
     CDS             complement(2725..3033)
                     /locus_tag="ACHA34_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793376.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3042..3461)
                     /locus_tag="ACHA34_002808"
     CDS             complement(3042..3461)
                     /locus_tag="ACHA34_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793377.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3454..3687)
                     /locus_tag="ACHA34_002809"
     CDS             complement(3454..3687)
                     /locus_tag="ACHA34_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793378.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3684..>4324)
                     /locus_tag="ACHA34_002810"
     CDS             complement(3684..>4324)
                     /locus_tag="ACHA34_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8793379.1"
                     /translation="PNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWR
                     LELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKN
                     TKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1223 a          961 c          620 g         1520 t
ORIGIN      
        1 atattctctt catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa
       61 ccattacctt catttaggcg cataagctgt tcaaaattta aaatcaacac atcatttgga
      121 gtgtgataat aaatattttc tttcagtttt ccgactatgg taattcggtc aattgaccac
      181 tgcattttta agggaagggt caaactttga tactttaacc gcctgttaga gggggctgtt
      241 aatttttccc actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag
      301 aaaaagagac ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc
      361 aaaaaatctc tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg
      421 agctagtgcg attagtttcc gtaaaggtcg agaacgtgtc cagcgttgct cgactttatt
      481 tttttgctct tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct
      541 atgcaaatat ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta
      601 acgctaggtt ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt
      661 agtggtttag ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc
      721 acatcgtaac taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt
      781 ttgacgataa aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat
      841 atccttttct gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt
      901 ttttaatcgc tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc
      961 cacttgtaaa tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc
     1021 tacatactca tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt
     1081 atcagcattt ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc
     1141 attcccagat aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat
     1201 atctttttgg aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc
     1261 ttcaatcttc actctctcag aagccaccag agccttttct cgctcatttt cttctctttg
     1321 cctagaaaaa tatacctcaa ctcatcagaa acgctctcac gctcttttac ggccgtttga
     1381 cgcaattctg aaatctcgct catgatttct ttatattcgt gaacatcttt gattttgtta
     1441 gtaccgacta actcacgctc ccaaccaaaa tctgccatca aacgttcaat tgactcaact
     1501 tcctgattcc taaaattatt ccaaatcact cgaccattct ctttatctgc tcgtaaaccc
     1561 tgctgttgca aggctttgtt aaaacttggt tgcttttgaa caccccgttt atacccttca
     1621 gcaacaggaa caatattcaa atgcaggtgc ggacttactt cgtcattatg aataacagca
     1681 ttgtagacgg ataaattagg attccgttct ttaaattctt tgaaatattc aactaaaatt
     1741 ttgttggcaa tcactcgatt ttgttctgct tgttgttccg atatgccagt agaagaacca
     1801 tcatcacgag tagtacgaaa atcatcaaga gtaccaattt gcacgataaa ttctcgctgt
     1861 ggttccaatt ttttatcctt gcgaaccttt gctaaatagt cctcgatttt tctatcggct
     1921 cttttctgct ttgcattata tttttctacg gcttcaccga ataattcttc atatttttca
     1981 cgaatatttg tttgttctaa atagatattt tcatccgatt ttgtgaaatc aatatgctta
     2041 tgccagtcat tgattttttg gctatcagtt aactcacgat tattatgaga aatgcttgta
     2101 ttctttgttc cctttttgaa cgaaatactg aaccccaagt taaaaccctc cttcggctgt
     2161 cgctaataaa aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta
     2221 attgtgacat ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga
     2281 caacgaaaaa aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc
     2341 ttcacgcaaa ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact
     2401 ctcactcatc tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg
     2461 ataatctcgt tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt
     2521 ttcgtgttgc tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc
     2581 aaacccaaaa cgcttccgaa gagccttgcc cattgcagca ttttctttct caatcaagcg
     2641 ttttttctgt tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat
     2701 atctgctaaa gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat
     2761 ttggtttaac tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc
     2821 agttgtttgc gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctaa
     2881 atcagcattg ataaccgagt tgagaacatc cataattcgt tcaagcattt cactataatt
     2941 atcttcatcc ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc
     3001 gactgtttct tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac
     3061 cattttcgat tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga
     3121 agcagggtca atttttgttg atcacgaatc atttctgtta attcagaaat atgtttgtcc
     3181 ttctcagcta attgatcacg caaaaactca ttctcttttt ctaaaggaaa atcactgcct
     3241 gtgttattga ccggtaattg accagtcaat tgatcacttt ttgaccgata aattgaccga
     3301 acaacaccaa tcaactcttt atctagcatg ataatgccat tttctttata ctgagcaagg
     3361 tcaaaccctg aattgaccag ttgattgcgc actgtatttt tcgatacacc caattcgtca
     3421 gcaagatttt ttatggtcat cttttctgac aaatcagtca ttagcttctc cactactttc
     3481 agctaccgca taaatatatt taacggcgtg cttagggata ataatcggag agtcaatttt
     3541 attatttttc aaaatcaatt catattgcgt tacatcaagt aacttaccgg acattttttt
     3601 gctatccaaa aaaacaacca caacgtaatc acctgttaaa tttaccaacc aatttttctt
     3661 accaaaatac ttttgttttt ctgtcatttt tcttcctcct ctgttacgtc tagccctaga
     3721 agccatgtgt ctaattcttt ttttaaatcg tctgccgatt ccgcaaaggt ttctcgcaaa
     3781 tgattcgtca attcatcatt ttgactttct tgcttcaaca aatccctgta ttttttcttt
     3841 gtattcttag ctagatttcc ccactcagaa tgatcataaa gcaaaccttt caccataatc
     3901 cgttcagtaa ctttagtatt tggcggaaaa taatgtggac tagcaaagct atccaaactt
     3961 tcatgaacca tctcatgcca atcagttgct tttccccttc gtaactgcaa ttctagtcgc
     4021 caccatgtcg ttatttccgg cggaacaatt ttacgttttc tttcttgttc cagtttttta
     4081 ttatacatcc gaatttgtcg ttcactggca cgactacccc aatatgccgt ttctaactta
     4141 ccagaacgcc cataaattgg tttaaaagac acaggatcaa ctacccgata ttgtgtaata
     4201 aactcatcag gtacatcgat aatgtcgcaa gcaatatccg cacgtgaaaa gtgaggttcg
     4261 ataaacaaat catgaataaa atttttcatg gaactagcta agaattgact aatcttattc
     4321 ggat
//
LOCUS       DAYYFU010000128         4282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000128 DAYYFU010000000
VERSION     DAYYFU010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.134"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..1257)
                     /locus_tag="ACHA34_002811"
     CDS             complement(37..1257)
                     /locus_tag="ACHA34_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8793380.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ACHA34_002812"
     CDS             complement(1277..1888)
                     /locus_tag="ACHA34_002812"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM8793381.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ACHA34_002813"
     CDS             complement(1931..2863)
                     /locus_tag="ACHA34_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM8793382.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="ACHA34_002814"
     CDS             complement(3284..4144)
                     /locus_tag="ACHA34_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8793383.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DAYYFU010000129         4036 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000129 DAYYFU010000000
VERSION     DAYYFU010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.135"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(242..391)
                     /locus_tag="ACHA34_002815"
     CDS             complement(242..391)
                     /locus_tag="ACHA34_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793384.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            853..1065
                     /locus_tag="ACHA34_002816"
     CDS             853..1065
                     /locus_tag="ACHA34_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793385.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1343..3142)
                     /locus_tag="ACHA34_002817"
     CDS             complement(1343..3142)
                     /locus_tag="ACHA34_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFM8793386.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3266..3862)
                     /locus_tag="ACHA34_002818"
     CDS             complement(3266..3862)
                     /locus_tag="ACHA34_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8793387.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1449 a          711 c          689 g         1187 t
ORIGIN      
        1 atattaaata ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt
       61 cataaaaaac gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag
      121 agagacaatt tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt
      181 acaaacgtct tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc
      241 cttatctaga ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt
      301 tattttcgtt ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc
      361 ggtattgcat atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta
      421 gttcttgaaa gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt
      481 tgatgcaatt aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct
      541 attttctttt aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat
      601 ataaatcata tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga
      661 acaacatagg aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata
      721 gttatctaaa aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacgttt
      781 tcaaatacaa ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa
      841 agagggaatg aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga
      901 gtatctcatg aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat
      961 gttgaaagtt atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc
     1021 gtaaaattct gtgatattag cgcacctgct gaattggttt cttaaacagt atttttgaga
     1081 attcgcaaga actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa
     1141 aaagaaaaag ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta
     1201 ttttagctat tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg
     1261 tcaaacaaaa aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac
     1321 ttttttcatt aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg
     1381 aataagtgat ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg
     1441 ttatgatacc atccacacca taaaacatca tgcgactcat agcatctgaa tcgttgattg
     1501 tccatacata tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg
     1561 tactatattc cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag
     1621 gtaagatgta gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt
     1681 acgtcaaact ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa
     1741 atcgttcgac aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt
     1801 ggtttagctt atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc
     1861 cgttttcagt aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat
     1921 ttacccctgt taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg
     1981 tttcctgaac atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg
     2041 cttctaaagt attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg
     2101 gttttgtctg aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga
     2161 aaaaaactgc aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg
     2221 cttgtgggat aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa
     2281 caatgatata aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta
     2341 aaagcgtcat agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca
     2401 tactttgtgc caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc
     2461 caatcacaat aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt
     2521 cccgaatggc tgctcgaaaa ggacgatctc gtagaatcat ttccggtaaa gcaaaaatca
     2581 gtcgaatccc aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc
     2641 aacgattagc aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat
     2701 cagaattaaa acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca
     2761 aaaaagtaat gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca
     2821 aagagattgg ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga
     2881 aataaactaa cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat
     2941 gttgagaaaa aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta
     3001 aaataaaacg ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca
     3061 tcagcaagac atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta
     3121 agctacgttt caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat
     3181 gaaaaaggaa agggtgcagt gtgttttgca gaccctttcc cagatttaac attgtcaatc
     3241 aatgactaat tttacaaaat aaatattatc caagaagacc acttagatat tgacctccat
     3301 aaatcaaggc catactatat aagaaaatcg acagtggttt agcaatcaaa atgattagtg
     3361 tatatcgttt cactgacatg tttgtcaatc cagccatcaa acataacgca tcgtcaggag
     3421 caactggaag aaaaatagct agagcaaaca gcttctcaaa acggttttga ttatccagcc
     3481 agccaatata tttgttatat gtcttatcgc taatcatgga aagtatgaat ggctttccat
     3541 aacgacgccc tagcagaaaa atgataatcg aaccaatgca gattccaata tagttataga
     3601 taaatccagc ataagggcca aatatcagga caccagctgc agtactgatc cctccaggaa
     3661 tgattggaat aactacttgc aggatttgga tgaagacaaa aataatcggt cctaaaatga
     3721 tggaatcacc tactaatcct cttaacgcat tcaaatcttt gaaaacacct aaattaataa
     3781 aataaatcgt cagcgcgatt gataggccta atccgataat ggatatgaaa tttatcagtt
     3841 ttcgagaggc agcaatattc attattcatc aactccttag aggttctttg ttaactatac
     3901 tttactagaa aagcaactaa ctgcgccatt ttttgatatg acagaaaaca aacaattttg
     3961 taagttcatc ataagtataa aagattgatc tcaaaaaaac atccaactaa agacttattt
     4021 aataacttgt tttttt
//
LOCUS       DAYYFU010000130         3969 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000130 DAYYFU010000000
VERSION     DAYYFU010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.136"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..618)
                     /locus_tag="ACHA34_002819"
                     /pseudo
     CDS             complement(1..618)
                     /locus_tag="ACHA34_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            654..1253
                     /locus_tag="ACHA34_002820"
     CDS             654..1253
                     /locus_tag="ACHA34_002820"
                     /inference="COORDINATES: protein motif:HMM:NF023433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793388.1"
                     /translation="MQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNTLYLDK
                     SLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAKGLFVE
                     KLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYESAMIQ
                     GFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIERT"
     gene            1282..1542
                     /locus_tag="ACHA34_002821"
     CDS             1282..1542
                     /locus_tag="ACHA34_002821"
                     /inference="COORDINATES: protein motif:HMM:NF023433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793389.1"
                     /translation="MDYQLPEGSRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDL
                     HRTFAQLPVELQPYAEMILNDVNNGELVIKEGWEFTDYLNEF"
     gene            2539..3507
                     /locus_tag="ACHA34_002822"
     CDS             2539..3507
                     /locus_tag="ACHA34_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8793390.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            3518..>3969
                     /locus_tag="ACHA34_002823"
     CDS             3518..>3969
                     /locus_tag="ACHA34_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM8793391.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFT"
BASE COUNT         1341 a          606 c          767 g         1255 t
ORIGIN      
        1 acccgattgg tagcgatgga agatcgtgaa tttcttttta aacgactgag gtaacgtaga
       61 tgaaattgtt ttaagggttt cttgaaagcc taccgtatct cgtttaacga aataatagcg
      121 taataattga caaaaatgat acgcagttcg caattcttcg ttgtagctta acagctcatt
      181 aacgatttct gtttgactaa ttggtcgttt aaacagtcga tgataatgcc gtttgagtgc
      241 ctcccatgtc gtggaatctt tgagtaagag tttccaatat cgcttgagcc gacggtactt
      301 cttctgattt tcagagtgtg aggttcgaaa acgattcatg atctggacac gtaattgatt
      361 gaagctgcga ttgatgtgtt ggacaatatg aaagcggtca gttacgatct ccgcatttgg
      421 aaagaccgtt ttaagcaact gacaatagct ggcattcatg tccatcacta ggtacttgac
      481 ctttaaacgg gcctttctag gaaagcgcag gaagtattga gttagtttgt aaagtctgcg
      541 atcttcaagg gtcgtaaata gctgattggt tgtcccatca acacaaataa aactcatgga
      601 accagaagtg gcaacattgt gtacttttct ttgcaatctt gtgtacaaaa ctattgcaaa
      661 acacagcgca taagaatcct tacaaaacag tagatgaagc aacccaatta gatcttgtta
      721 tagttgttaa tcagctctta acgggttacg attcaaaatg ggtcaacacc ctatacttag
      781 ataagtcact tgaatatcag aacataattc aggctttttc acgcacaaat cgtcttaatg
      841 gtcctgaaaa accgttcgga accattcgtt attatcgtaa agcacatacg atggaacaga
      901 acattgaaga ggctattgag ttatactcag gcaaacaagc aaaagggtta tttgttgaaa
      961 aattatataa taacttagaa aagttgaacg atatattcga agaaattaag tccgtattta
     1021 atatttctgg catagaaaac ttcagtcatt taccggaggt tccaggggaa attaagcaat
     1081 ttgtaaatct ttataatcaa ctaatccatt gttatgaatc agcgatgata cagggattca
     1141 catgggcaaa atcgacttat gaaatccagg attatgaacc tataacagta gtaatcacag
     1201 aatatgaatt taatatcttg gcacaacgtt acagcgagat agagagaacc tagagacgga
     1261 aatgatagta tagcgtatga cattgattac caattacctg aaggtagtcg tagtcgtatt
     1321 aattatgagt atttaaacac aaacttcaaa aaatacatga atataaaaga agaacgtgat
     1381 tcgaatttaa ttcaatcatt gttaaacgac ttacatcgca catttgcgca attaccagtg
     1441 gagttacagc catatgctga gatgattcta aatgatgtaa ataatgggga attagttatc
     1501 aaagaaggat gggaatttac tgattacctc aatgaatttt agtggattca accaatatta
     1561 atgaggcatt attacgtgag atgttggatt tacgcttaac cgaggtaaac attaatgaat
     1621 atagtcgatt tgataaatta aaatcaagtg ttaacgtagg tcatttcagt ggatatatcg
     1681 agaaaaattt aggtaaaatg gtaataccaa tcaaggtaaa tatgctaatt gacagattat
     1741 tgcgtgcatt tttattagaa gatgtattcg atgttacaga gtacataaag aattacttta
     1801 actagagtac acctacatct tatagtacat aaaaaaattc atctttttta gtttaataat
     1861 tcaccaaaaa ggtgagtaat gccatttttg aaaattgagc ttgttttatg tctacatctg
     1921 tatccgtata taatccaaag gattatcaac tcagaagagt ctttattttt tctgaatata
     1981 actactgtat caaaaacaat cgattatgag acaaaacaaa atgacctctt ttatttaaga
     2041 aaggaggtca ttttttgtat cataaattgt attaaaatca atgtatcact ttttaatcag
     2101 ttttaagacc ataaattgcg tactaaaaga aattctgcaa ttcaaaataa agcattttaa
     2161 gccattttca aatttaagtg cacccatcta cccgtcaaat cactttattt tccactccag
     2221 tagtttccta attcaactgt gtgcaaaaac gatttcttct aaacaaatca agacaaactt
     2281 ataaaccttc atcccagcac cataattatt taaatcatag gcatttttcg aaattagtat
     2341 atgtactcaa gtttcactgc agattattta tattttttag agaaataaat ttttcctatt
     2401 gacaaacaca aagaaagcgg atacaatgca attatgtcac atgacgttat atgacgttat
     2461 gtgatatctt tacatttaat cagttacttt tattttggat aaaattaaca tttttaaaat
     2521 tttaaggagg aaatgttgat ggatgtagca aaaacggtta acgcaattgt agaaaaaaat
     2581 gatggagaga tttctggaat ttatttggtg gcttgtggcg gttcgctggt agatatgtat
     2641 gtctcagatt attttcttaa agccgaagca tcgaagtgta ttacaggctt atatacagcg
     2701 aacgaattta accatgtaac cccaaagcaa ttagatgata aatctgttgt cattctatgt
     2761 tctcatggag gaaatacagc agaaacggta aaagcaggag aagtcgctca agctagagga
     2821 gctgtgactg ttggtctgac tcataataaa actgcggatt tattaaaggt ttctgatcat
     2881 agtttccttt acgaatgggg agatgatgca aaaatagtca ataatcctat ggccatcatt
     2941 cttgatttaa ctgtatcttt ggtcaatgtt accgaaacat ttgaacatta cggtaaattc
     3001 agagaaggca tgaaggttat cgataccgta attgaaaatg gcattaagca ggtgcaggat
     3061 cgtattaaag tgtttgctga taagtacaaa gatgaaacaa tgttttatat cttaggtagt
     3121 ggaccatcat atggacatgc ttatggtttt tcaatttgct ctttgatgga aatgcaatgg
     3181 cttgattcaa cttctgttca ttctggagaa ttcttccatg gaccttttga agtaacagac
     3241 aaagatacga attttattct cttgatgagc attggacgca ctcgtgcttt agatgaacgg
     3301 gcaaaacgat tccttgataa atatgcagaa agagtagaaa tcgttgatgc aaaggaacta
     3361 ggattggata tgatatcaga tgaagtttct gaattcttca atcccatttt attctacagt
     3421 attctttcag aatatcgttc agctttagca gatgtacgca atcatgattt agatgttcgt
     3481 cgctatatgg ggaaagtgga gtactaggag gattaaaatg aaggtattag gcttaggtga
     3541 taacgtcgtc gataaatatg aaaatctggg aattatgtat cctggaggaa attcattgaa
     3601 ttttgcagtt tttgcaaaaa aactgggaca tgaaagtagc tttatgggag tttttggctc
     3661 agatgaggag gcagctcatg ttttatcagc aataaaagaa ataggactgg acaacagtca
     3721 ctctcgcttt gaaaaaggcg agaatggctg tgccagagtg aagattgatg agggcgaccg
     3781 gattttcctt ggcagcaatt cgggaggtgt tactcgtgaa tacccgattc agcttactga
     3841 agaagacaga gaatatttaa accagtttga attgattcat atgggattat atagccatgt
     3901 caatcatcta ttagaagagt tacaaaatgt ttctggaaag atttcttatg atttttctga
     3961 cgactttac
//
LOCUS       DAYYFU010000131         3925 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000131 DAYYFU010000000
VERSION     DAYYFU010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.137"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(573..1130)
                     /locus_tag="ACHA34_002824"
     CDS             complement(573..1130)
                     /locus_tag="ACHA34_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793392.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="ACHA34_002825"
     CDS             complement(1309..1920)
                     /locus_tag="ACHA34_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793393.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="ACHA34_002826"
     CDS             complement(2033..2290)
                     /locus_tag="ACHA34_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793394.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="ACHA34_002827"
     CDS             complement(2887..3165)
                     /locus_tag="ACHA34_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793395.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="ACHA34_002828"
     CDS             complement(3199..3450)
                     /locus_tag="ACHA34_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793396.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1195 a          799 c          520 g         1411 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgtat
//
LOCUS       DAYYFU010000132         3875 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000132 DAYYFU010000000
VERSION     DAYYFU010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.138"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..2257
                     /locus_tag="ACHA34_002829"
     CDS             158..2257
                     /locus_tag="ACHA34_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFM8793397.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2453..3316
                     /locus_tag="ACHA34_002830"
     CDS             2453..3316
                     /locus_tag="ACHA34_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFM8793398.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3421..>3875
                     /locus_tag="ACHA34_002831"
     CDS             3421..>3875
                     /locus_tag="ACHA34_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8793399.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         1543 a          646 c          782 g          904 t
ORIGIN      
        1 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
       61 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
      121 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      181 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      241 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      301 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      361 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      421 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      481 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      541 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      601 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      661 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      721 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      781 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      841 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      901 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      961 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
     1021 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1081 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1141 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1201 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1261 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1321 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1381 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1441 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1501 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1561 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1621 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1681 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1741 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1801 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1861 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1921 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1981 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     2041 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2101 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2161 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2221 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2281 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2341 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2401 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2461 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2521 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2581 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2641 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2701 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2761 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2821 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2881 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
     2941 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
     3001 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
     3061 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
     3121 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
     3181 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
     3241 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
     3301 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
     3361 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
     3421 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
     3481 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
     3541 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
     3601 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
     3661 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
     3721 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
     3781 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
     3841 catggaatct gtcaggaatg ctatgaaaaa gaaaa
//
LOCUS       DAYYFU010000133         3830 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000133 DAYYFU010000000
VERSION     DAYYFU010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.139"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1355
                     /locus_tag="ACHA34_002832"
     CDS             <1..1355
                     /locus_tag="ACHA34_002832"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFM8793400.1"
                     /translation="ATIVDILGYAIAPEYLFNVLADQAKQATFQLNDLNKAFVQLAST
                     YNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLIS
                     QFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLT
                     HPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVV
                     MNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHG
                     VLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDA
                     SREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDT
                     FEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ
                     "
     gene            1345..2562
                     /locus_tag="ACHA34_002833"
     CDS             1345..2562
                     /locus_tag="ACHA34_002833"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM8793401.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2575..>3830
                     /locus_tag="ACHA34_002834"
     CDS             2575..>3830
                     /locus_tag="ACHA34_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM8793402.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT         1319 a          659 c          799 g         1053 t
ORIGIN      
        1 ttgccacaat cgttgatatt ttgggatatg caattgcacc agagtatctt ttcaacgtct
       61 tagcggatca agcaaaacaa gcaacgttcc aattgaacga tctaaacaaa gcctttgtgc
      121 aattggcatc tacctacaat caatttaatg ggttatttga tgatgtggat ctccaatcaa
      181 aaaaactagg aacagatgag caacaacgaa atgtgacgat taccgaagtc atcaagaaat
      241 tgaatgatgt agatgtttta gaacacgatg gggacgtgat cggggatgcg tatgagtttc
      301 tgatcagtca atttgcttca gaagcaggga agaaagccgg agaattttac acacctcata
      361 tggtttctga tatgatggcg caaatcgtaa cgctcgatca aaaggaacga cgattcttta
      421 gtgtttttga tccaacgatg ggttcaggtt cattgatgtt aaacgtacgt aactacttaa
      481 cacacccaga taacgttaaa tatcatgggc aagaactaaa tacaacgaca tacaatctag
      541 caaaaatgaa cttgatttta catggtgtcg acgccgaaga aatgaatctc cgcaatgggg
      601 atacgttgaa taaggactgg ccaacagatg aaccttacac atttgatgca gtagtaatga
      661 acccacctta ttcggctaat tggtcagcag atacgacttt cttagatgat tctcggttta
      721 atcgttatgg gaaattagcg ccaaaatcca aagcagattt tgcgtttctt cttcatgggt
      781 tctatcattt gaaggaaaca ggtaccatgg ccatcgtctt accacacgga gttttattcc
      841 gtggcgctgc agaaggggtc atccgtcaaa aattactaga agatggcagt atttatgctg
      901 tgatcggtat gcctgcgaac ttgttctttg ggacatcgat tccaacgacg gtgattgtgt
      961 tgaagaagaa tcgtcaaact cgagatgtat tattcattga tgctagtcgt gaatttgtta
     1021 aagggaaaaa tcaaaataaa ctatcggaag aaaacattca aaaaatcctc gaaacctacg
     1081 ctgaaagaaa agatgtcgag aaatacgctc atttggcaac ctttgatgaa atcaaagaaa
     1141 atgactataa cttaaatatc ccacgctatg tagatacttt cgaagaagaa gaaccaattg
     1201 atatggttca tgtcggaaat gacatcaaga agatacgaca agaacagcaa gtgctagaaa
     1261 aaaaactttt agaagctatc tcttccctgc aaacaacacc tgaaaatgaa gcgtggttac
     1321 aaggtgcgct ggaggtgttc aaacatgagc aatgatacac aaccagaaat ccgtttccca
     1381 ggatttacag atgattggga acagaggaag ttgggggact tcgttgtaga ttatgttgaa
     1441 aagacaagtg tacaaaatca atttcctatg ctaacttcat ctcaacaaaa agggatagtt
     1501 ttgcaagaag actattttgc aaatcggcaa gtaactacag aaaataatat tggttacttt
     1561 gtactaccta gaggatattt cacgttccgt agtcgaagtg ataatgatgt gtttgtgttt
     1621 aaccgtaatg acattattga tagagggatt ataagctatt tttatcctgt atttacttta
     1681 aaatctgctg attccgactt cttcttaaga cgtattaaca atggaatcca acgacaatta
     1741 tctattcaag cagagggaac aggtcaacat gttctgtctc taaaaaagtt taaaaatata
     1801 gtagcaatgt ttcctagtga ggaagaacaa caaaaaatcg gcaccttctt caaacaactt
     1861 gacgacacga tcgctcttca tcagcgtaag ttagatttgc tgaaggaaac gaaaaaagga
     1921 ttcttacaaa aaatgttccc tgaaaacgga gcaaaagtgc cagaaatccg gtttcctggt
     1981 tttacggaag attgggaaga gcgtaagttg ggagaattag gtaaaactca atcgggaatt
     2041 ggatttcctg atgccgagca aggtggttct gaaggtactc cattttttaa agtttccgac
     2101 atgaataaca ttggtaatga gtatgaaatg agaaatgcaa atcattatgt atctaatgag
     2161 caaattgagc gtaaaaaatg gaagccaata aaagatgtgc cagcgattat atttgctaaa
     2221 gttggcgcag caataatgct taatagaaaa cgtttggtca caagcccttt cttaatagat
     2281 aacaatacca tggcttattt atttaataat acttgggata tctattttgg taaaatattg
     2341 tttgaaacta taaatttgcc aagatactcc caagtagggg cattgccttc ttataacagt
     2401 tctgatattg agaatatcag tgttaaggtt cctgtaaagg atgaacaaca aaaaatcggt
     2461 actttcttca aacagctaga tgatacgatc gctcttcatc aacgtaagtt agatttacta
     2521 aaagaaacga aaaaaagctt cttacaaaag atgtttgtct aggagggaag aagaatgaaa
     2581 attagtcacc gtgaggaagc agaagtcgaa gaacagttga ttcgtgtact tggagaaggg
     2641 cataatcagt ggacgtatcg tccggatcta aaatcagaag aagacctttg ggtcaattta
     2701 cgccaaaaaa ttatctcaaa taatcaagca gaactgaatg attttccttt gaccgataaa
     2761 gaatttgaaa cgatcaaaac ggaattactg ctgcgaacga aaaccccatt tgatgctgcg
     2821 aaatggttaa aaggtgaaaa tgggatggct cggatcacga ttgaacggga agattcgcaa
     2881 ttgggttctg tctcacttat cctttattcc aatcaagaca ttggtggagg aatctctact
     2941 tatgaagtgg tccatcaaat tgcaaaacgt ggaagtaaca tagaagcacg ggatcgacgt
     3001 tttgacgtca ccttattaat caatggatta ccgattgtcc aaatcgagct gaaacaagta
     3061 actgcaaaag atggtgttta tcaggcattt aaccaaatca aaaaatatgc agaagaaggt
     3121 atgtttcgga acaatatctt ttcgacctta cagttgtttg tggtttccaa tgaacaaacc
     3181 actcggtatt ttgcaaatgc cttaccgaaa gacttgcacc ctaagttttt atttagttgg
     3241 cgaacgaaag acaatgaaaa agtagaaaac ttatatgagt tttgtaaaca agtgttaaat
     3301 atcccggatg ctcatcgttt gattgcggat tatacaatcg tcagtgaaga tcaagacaat
     3361 aaaaccctca tggtgttaca tccttatcaa gtacatgcga ttcaagcctt gtttattgcc
     3421 gcaaataaac atcaatcagg gtatgtgtgg catgcgactg gttcaggaaa gacgttgact
     3481 agttttgtat ctactaagct tttagcacga aaatcgggga tcgatcgaac gatcatgtta
     3541 gtggaccgca aagatttaga taatcaaacc accacagagt ttacaaaatt tgcttcggaa
     3601 tttaatactg ggatttcttc cgggaacgcc aaagcgaata gcttgatcgt tggaacggga
     3661 agtgccaaag aattaagtga aaccctgttg gcagatgcca atgcaaatgt tgtaatcatt
     3721 acaacccgtc aaaaactaga tgcggcgttg aaatatgcaa agaaacaaga agagaaaaaa
     3781 ggaaccaatc gcttccaaaa attaatgggc caacatatcg tgtttgtagt
//
LOCUS       DAYYFU010000134         3818 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000134 DAYYFU010000000
VERSION     DAYYFU010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.140"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            503..649
                     /locus_tag="ACHA34_002835"
                     /pseudo
     CDS             503..649
                     /locus_tag="ACHA34_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(737..1030)
                     /locus_tag="ACHA34_002836"
     CDS             complement(737..1030)
                     /locus_tag="ACHA34_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFM8793403.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            1067..1252
                     /locus_tag="ACHA34_002837"
     CDS             1067..1252
                     /locus_tag="ACHA34_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793404.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            complement(1433..2611)
                     /locus_tag="ACHA34_002838"
     CDS             complement(1433..2611)
                     /locus_tag="ACHA34_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8793405.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2715..3029)
                     /locus_tag="ACHA34_002839"
     CDS             complement(2715..3029)
                     /locus_tag="ACHA34_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8793406.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3136..3354
                     /locus_tag="ACHA34_002840"
     CDS             3136..3354
                     /locus_tag="ACHA34_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793407.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            complement(3274..3744)
                     /locus_tag="ACHA34_002841"
     CDS             complement(3274..3744)
                     /locus_tag="ACHA34_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM8793408.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGFPYFDFGWHIFHAKFCDFNSIGKI"
BASE COUNT         1219 a          726 c          558 g         1315 t
ORIGIN      
        1 ttattctaag gttattttag tcttgtcctt taatacaaag attttatttt taaataaaaa
       61 atactctttt tttacgatta ttgaaaaaaa aagataaaac ttgattaatt gaagtattgc
      121 ttaaatttgg aagctatgag ggaagaataa aagagttagg acgggattat ctactatttt
      181 tctttgtttt aaatatttgt tatctttgaa cataaaagag aaatgtgtcg actgcagtgt
      241 tgtgtccctc atcacgaata aacggtgttc aattagctga tcttgtattc acttaaaaaa
      301 tggtaaagta cgagttgcta ttccccattt tcaattttct aacgctcagg acaaaatact
      361 gttttcacaa cctcattgga ataaaagcat atatattgtt tcaagaacga aatcttgtta
      421 tctttttaaa gggcatctgt ccaaaatcta attttaggag gtacgttttg gtatttattc
      481 gtacaaataa aagagaatta tctttattca tgtaacgcat ccaattgatt ttttcatgta
      541 cgctcctgga acgattttac tgacgattgc ttatttaact gcgaatcgct cattagcttt
      601 tatcatagct atccatattt tgaataacgt tcttggcttt gtattataga tacagatgac
      661 aaaaaaagtt caagtatgcg gtgtagaacc aatatcgcat acttgaactt ttttcaatga
      721 gtataaatta aaacttctaa ttttcaattc ccatcatctg atatgcccga ccgtctgaac
      781 ttcttttgaa aaagccaaat gtgattagat aacgtctttg ttcgacataa tcgccactag
      841 aaaatacgtt cagcagtatc gaattaacct ctgtttctgt ataagtcact ccatggtcaa
      901 agcgtttcaa gacttctttc aataggatgt atttcttttt tgttgaactt ggtacttgtt
      961 ttacttttcc atcattcatg aatgtgtcta aaactttcat cttttcttgc tccgtataag
     1021 tataaaccat cttctttctc cttttgattc taagaatact ccatttatgt tagaatattc
     1081 tggatttttt attaaagcgg ttactcttaa gaaagatatt atcatagtgc tctttttttt
     1141 actactactt ataggtagat ttattctaat ccattattct cccctaccaa taattaaaga
     1201 aaaaactgga tcagggtgtt caaattacat gctaagaaaa aagaaccctt gaataccaag
     1261 ggttctttct cagtctgata acaatgatta acgacggatt tctttgatac ttgtgaaaag
     1321 tcgttaaagt attgatataa aaggattttt attacctctg actacatatt gactaatttg
     1381 ttaaaaaatc gtttttcaag aatcctgtaa taccagtgct ttcaccgttt ttttaactct
     1441 ctaaagaact aaaagcaatc agaggtttta ccttttcatt tgacgttaag tggcgataaa
     1501 ttttcgaagt tgtttggtca gtatggccta agcgatcttt caagacttcc cagctacagt
     1561 gttccgctac taagcttgcc attgtatgac gagctaaatg agtatgaaat ggcttattaa
     1621 ttcctgagcg attcacgcac cgtttgacgt gatcatttac atactctcga cgataaggaa
     1681 caccacgttt actagaatca agatacacaa acaaatatgt ttcttctata ataaacctgt
     1741 aatgttgcct catctcaccc aaagccttaa tcaactgttg tagcttttcc ataactagag
     1801 gagttactag aactcgtcta gcagaactaa ctgtcttagt agtttcaatt cgaaaatcct
     1861 tactcttacc tttaactgcc gtttgcttat agaacagaat ttcatttctt ttgaaatcta
     1921 tatctccagg ctgtagagca agcgcctcgc tgactcggca gccagtgtaa aatagaacat
     1981 ggaaaagtac agacatcggt aattcctcat gaacaatgcc ataattcaaa aaagttctca
     2041 attcatcgac agtaagaaac ttgtcctcaa tattatttat ggcttctttt aaatcagtta
     2101 ccgttaactg atattttggc attctcacag acttcattgg attttcactt atcaaattct
     2161 ttctttgaca atatttaaaa atcatattta gaacttgaat aagtgattgt aaactatttt
     2221 tggcatagcc aacatttgtt ccattaacgc cacattcctt taaggtaaaa agcaattctt
     2281 gaaggcactc tggtgtcaaa tcagacaact ttttatcacc aatccatcga acaataattt
     2341 ttaattgttc ctctcgctta tcataggtta tttccttttg ttcatttaac gaacggtaat
     2401 attggaacca ttgctcacct acaacactaa acttgtcatt gcttgcaaca atttttttct
     2461 taagcctttg ttgtttctcc atttccagat aagcgagttg cgcctcacgt tttgaattaa
     2521 agccacgtct tgttgttcga tatgcttgaa agtaccatgt gatcctacca gtagaactga
     2581 tttgcttctt gaatcgatct gttttttcca tttattgatt cctcctattt tgaaggaacc
     2641 caacaagcag aaaccctttg gaaatctttc tatctatgtc atcattgtac cgcatcttag
     2701 aaagaaaatg taacttataa agatcgaagt agtcgcaatt tttctcgata gtcattcgaa
     2761 tcactctttc tcatccattc taaaacatca tcacgaaaat agtgtcctcg aatcccgtac
     2821 caacagggag gaaactcacc aagttttact aaacggttca gtgtactatc acttatctga
     2881 aatagtcgct ttaaatcagt atttgttaat accagaggta aatgactgac tgcaagcaat
     2941 ttttccgtgt aatcttccag ttttttttgt aaaattgaat agaactctat ttccagagaa
     3001 ttaacatttg ttttaacttc caactccatc acccttctct ctcaaaatca aaacgaccac
     3061 cacggtaccc cttaaaattg taaaaaaaca ctccagtact ccatcaaaac ggactagaaa
     3121 agaggaattt ttaatatgaa acaaggaatt gcagacataa aaataattaa agaaattctt
     3181 gaaaaatcta cggctaatgc aatagcattc ggaacaggaa tcaacttgag tacagtaaaa
     3241 aaacttaaaa gtggagagag agctgaagaa aaattaaatc ttgccgatgc tattaaaatc
     3301 acagaatttg gcatgaaaaa tatgccaacc aaaatcgaaa tatggaaatc ctagctcttc
     3361 caaagccatt tgtcgttggt accaaacact gccgagactt ttcccaataa tatcgccaat
     3421 ttcacgatca ttaagaccga tgaagaagga cataaaaatg atttcttgtc tttgactagg
     3481 taatgctgca atcgcatttg ccaactcctc attggaaata aagattttca taccaaggac
     3541 aataaaagta ccactactat tcaaatttgg ttccaaataa tagaactctg tacctaaccc
     3601 tttttgttcc aatatttcca ttgatatttg ccgttggcga aatctagcgt acttcttttg
     3661 aatatttaac gcctcattgc agactacttt cttacaaaaa ctgtcaaacg tatgttgaat
     3721 gtgggtttcc tctgagtcgc ccataatatt ctccccctca aaaaattcat tcaactcgtt
     3781 ttacttctat ccctttccat tattactaca atctagga
//
LOCUS       DAYYFU010000135         3806 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000135 DAYYFU010000000
VERSION     DAYYFU010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.141"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..534
                     /locus_tag="ACHA34_002842"
     CDS             37..534
                     /locus_tag="ACHA34_002842"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFM8793409.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(919..992)
                     /locus_tag="ACHA34_002843"
     tRNA            complement(919..992)
                     /locus_tag="ACHA34_002843"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(956..958),aa:Ile,seq:cat)
     gene            1179..1502
                     /locus_tag="ACHA34_002844"
     CDS             1179..1502
                     /locus_tag="ACHA34_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793410.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            1666..1920
                     /locus_tag="ACHA34_002845"
     CDS             1666..1920
                     /locus_tag="ACHA34_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793411.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            1990..2235
                     /locus_tag="ACHA34_002846"
     CDS             1990..2235
                     /locus_tag="ACHA34_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8793412.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            2311..2676
                     /locus_tag="ACHA34_002847"
     CDS             2311..2676
                     /locus_tag="ACHA34_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFM8793413.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(2737..3300)
                     /locus_tag="ACHA34_002848"
     CDS             complement(2737..3300)
                     /locus_tag="ACHA34_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793414.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINLRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         1167 a          680 c          615 g         1344 t
ORIGIN      
        1 gaataaaatg acgatatgaa tgtaggagga aagaaaatgt tagttattat tgatatgcaa
       61 aatcatattc ttgatccaaa cagcgaattt tatattccag agtcagatca attggcagaa
      121 cgtattgctc aaagattagc taaagcacgc caaaataatg aatatattct cttcacacgg
      181 gatataccag ttgaactgaa ggacaaagaa gaagaacgag aagatttgca attgatccct
      241 cttctttctc cacaagaaaa tgaacgagtg attaaaaagt attactttac gattccacct
      301 gaaactttag ctgaaatcca ggaaacttta tttgaaagca aagaagaaca aaaagagatc
      361 gaagtagtcg ggattgaaac aaacttgtgt gtactctcta atctgatagg cctgcagagt
      421 gcttttcctg aagcagactt ttttgttgat ccaatgttag tctccagtag aaaacacgga
      481 gaatccgctt tagagctgtt gaaagatttc aacgtctcta tcgtcgaatc ataaaaatat
      541 ctatcttcat ttacgatatt taacacacaa taaaaaaagc acgcagaact gttttagtcc
      601 tacgtgcttt ttctatcaaa gtagattggc ttttttatga cacagagtga aacggcaggc
      661 tcacgacctc tttcttaaat ggtgttcaat cagctgaccc tcggtattag cttaacaaac
      721 ggcaaaatat gaggaactat ttttccgttt gttaagctaa tactcaggtc aaaacgctga
      781 tttcacaacc tcttccttaa acggtattca aaagcttgca tctgcagtaa taagaacagc
      841 caagtaaaaa cataagaagc tgtttttact tggctgttct tattacttga tgcagggcag
      901 ctttttcaca acctcttatg taccttgtag gactcgaacc tacgaccgga cggttatgag
      961 ccgtctgctc taaccaactg agctaaaggt acatcataaa taaaatttat ttttgttact
     1021 tttcaactat agcacgaatg acaaactctc acaagtttca aaatataagt aaaataatat
     1081 tgttattctt atttctttta cttgtgtcat aactattttg ttactttaat ttttatttgc
     1141 ttattttaga aaaaatgtta ttcttttatt tattctcaat gttaggagtg attattttgc
     1201 catttattgc gattttattc gatttagttg cggcagcagc ttattttctc caattaaatt
     1261 atcaaacctc aggaattctg tttattggaa tgatttttca aggtgtgatt acccttttgc
     1321 ttcttgtcat gatgctcagc tataaaggaa aaagatatgc ccgtattcaa acaaaagtat
     1381 ttgttaaata tgtttccatt cgctatggga tcatcattct ttccttttta gtgaatgcga
     1441 tcgtcctctt tttatatgta ttgaattact tagatatcaa tccattgatt ttttctcgat
     1501 agataagaca cattcagcgg aacagttcct gcttatcagc agaaaactgt tctgcttttt
     1561 catttttcat tgatctattc ttcttttatt ttcctagcat ttcatttgtg atgtatgcaa
     1621 taacatgtta tcttttatta tatgaatcgt tgaggagtcc gttacatgat tacgttggtg
     1681 agagttttgt tttgggtgcc tgcggtcgca ctggtcgcaa gtattgttta tcttatgaat
     1741 tggaacaaag aacggtttta tttagctatt ttgactcttc cggctatcta ctttatgtgg
     1801 aaagttttta attacaacta ctttgaaccc gatagtgttt ttatagaaga attatcagga
     1861 ctggttttgt ctttattgat cgtcattctt tatttgatac gtttaaataa aaaacattga
     1921 gtactattga ttgcatgtgt agctttctat agtcattata actattctac tataatgaaa
     1981 ggtgattaaa tgagtatcga agaagctatt ttactggata ctttacgttg tcgtctgaat
     2041 ttcttaagaa ctggccgatc tttatctcaa gtagctgttt ctagcgcgtt aagtattcct
     2101 ttattcaaat atcgtaagta cgaagaagga gaagaaattc caagcatcat tgaattaatc
     2161 tatatcgctg acttttacga tatttccgtt gattacctaa ttggtcgctc agaaacagca
     2221 agcaaactga agtgattttt tccagccttg gtgctggttt tttcatttaa cgtctaaggt
     2281 taacaatcta tatataaagg agacatttct atgcgattat ggcatgaaaa attgatttca
     2341 aagcttcctc gtcaacagct tttaggacaa catcgggaat gctgtgcatt aagaggaaat
     2401 ggctggggaa agaaacatgc tactgtggat tatgtttttc actattctcc ttatagatta
     2461 ttccaatatc atcagcttgt catggatgaa atggaaaaac gtggttacag cccagcacca
     2521 gagtggaaaa atcctatgta tcgtggcaaa tcatgcgaag cattttttga tttacctact
     2581 gtagagcgca acgtgccgat ttatccggaa cacgatgaag tttatctagc agaatgttta
     2641 gcaaatttgg agcaaaaagg aatttatctt acctaatata actagaaaag ccttctatct
     2701 cctcttactt cattaaaaag ctttaaccat cttagatcaa gctaaaatgt cgtaagtttt
     2761 agggttatct tcatcctcac gtgcaaaact aaaagcgtac tctagttttg gttcatcgcc
     2821 aacttcccta gacataaatg aacatctcct gacgtggttt aaaagtaccg catcttcgac
     2881 gtctgtatac gatgcacagc acagattgct tcttttgatc tttcttttga tttttctgtt
     2941 gcttcttcta caccttcttc gattactaca tcgatgctta caattgcttc tgcctaatat
     3001 tcccatgcat cgtcgattta agcctctgta gtcactgtga tcgtttccac attttaactc
     3061 ctctgtcgtg gcttcagttg attttgtgat cagttcagtt gctaagccag agtcagaaca
     3121 acctgccatt gctagcgtta atattgatag taaaaatagt ttttttatca taaggttccc
     3181 cccctgaaaa tagactaaaa taataacgct ttcttttttt gttttcagac gaagattaat
     3241 aatttctaaa tattctttaa aaaagcttat aactattatg ccttttttaa tatataacac
     3301 agttattttt aaaaataaga atgctaaatc actctacaat aacggcgaaa gcacaaaaaa
     3361 taaaaaaatt attacgttat taaaaaaaca gatgaaagat caggttttat tagatctcat
     3421 tctttcatca cttgaaggga tttaatgaac tactttttcc tctttgtcgt ttttatatat
     3481 tcgtattcct tcaaacagta ggatagcctc tttcaatgct tgatatttga actgtccgtt
     3541 acttaaaaat ttagatgtgc cgccctcttc ctagaacata ttctttttta ctttttcatt
     3601 aattgacaga ccaatcgaac gaaactgtaa atttcgatag gataatgatt ggtcaaactc
     3661 attcattttc aagacatctc gattcatatt caagatagga atctttttat caacatcact
     3721 ttattcgtat tttcctctac cataaaagct tcacgctcca caaaattgtt ttatacacta
     3781 ctattcagcc cattcaacga ccaatg
//
LOCUS       DAYYFU010000136         3713 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000136 DAYYFU010000000
VERSION     DAYYFU010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.142"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACHA34_002849"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACHA34_002849"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFM8793415.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="ACHA34_002850"
     CDS             complement(2261..2512)
                     /locus_tag="ACHA34_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFM8793416.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="ACHA34_002851"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="ACHA34_002851"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFM8793417.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="ACHA34_002852"
     CDS             3462..3680
                     /locus_tag="ACHA34_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793418.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          797 c          688 g         1203 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aag
//
LOCUS       DAYYFU010000137         3496 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000137 DAYYFU010000000
VERSION     DAYYFU010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.143"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..255)
                     /locus_tag="ACHA34_002853"
     CDS             complement(<1..255)
                     /locus_tag="ACHA34_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793419.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDD"
     gene            complement(257..1684)
                     /locus_tag="ACHA34_002854"
     CDS             complement(257..1684)
                     /locus_tag="ACHA34_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFM8793420.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            complement(1677..2639)
                     /locus_tag="ACHA34_002855"
     CDS             complement(1677..2639)
                     /locus_tag="ACHA34_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8793421.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            complement(2672..>3496)
                     /locus_tag="ACHA34_002856"
     CDS             complement(2672..>3496)
                     /locus_tag="ACHA34_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM8793422.1"
                     /translation="VHNQVYFGGGINLWLAKKNGIRQRIAHSHATEDGKSQNIVMNVL
                     RKFLTKLLLQNATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYSKNSEAKVNKRKEL
                     DISMSTFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEGSLRNKLKAKAVE
                     LDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLADTISKD
                     TKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTTEQMESIYSRDH"
BASE COUNT         1246 a          590 c          399 g         1261 t
ORIGIN      
        1 atcatccgag aaaaattgat tatggttagt taacaagtta attttacgtt taaaggaaga
       61 accgattttc cggtgattca caaaaacaac atcattaaat agccctaatt cttttatacg
      121 ctcactatat cttctagcgt tttcaaattc agaataaata aataaatctc cttttgattc
      181 tcggtaaaaa ttttgataaa catttaatat attaatcaaa tgtaaaggag tccaagcaat
      241 gaaagctgtc ttcatattaa ttctcctttt ttaaatacaa gtaagaaatg cttcccaaat
      301 aataccagaa aacaattgaa atgacatcgc gcttattgaa aagtacatga gcctccacca
      361 tttcatctat aaaaatagat agtataaaaa ttaaaacaat accaagaaac gcataattca
      421 ctttacttct aaaaaatgtt ttgagtaaac taaagatatt taacacatag aaagcataga
      481 tacatataaa agcaattata ccacttgata caagtacttg aagaaatcca ttatgcatgt
      541 ttccatgtgc tctttttagt tcattcacat ctgtagtatc aagatctact tttttcaaaa
      601 attttgcatc gctattccca tagaaatcgg tctctccata accaaataac aaagttggtc
      661 ttataacttt aagcgcagct ttccaaatcg taaaacgacc agctgaaatt tcggagttat
      721 cactagaatg ctgtaatatt acttcttttt ctggaagtgg agttttatct gattcatttg
      781 actctataac ttctactttt ctatcaatcg atacaggaat tgttgctaaa aaatccttag
      841 cataattagt accaaaagaa aaatatacca atagaactaa taatatgctt accatccatt
      901 tgatttttac aggtagtact tctctaatgc tctttctaaa ttttgaattg gcaaacaaca
      961 aaagaaataa tactaaaaaa gaacaaatta ctacctgggc acctctagac gaagaaagtg
     1021 taatataaaa tatttgtacg ataaaattca tcatataaaa aacacgaatc gtttttgtta
     1081 tttttgaaat taatacacta attaaactta aaataataga agtatatcca aacatagctc
     1141 caacatttgg acttgtatag acaccaaata accgatgttc aataaaacct tgtcttgcaa
     1201 gtaatcctgg cgttcctgtt gggacatgat aagaaatcaa aaaaataaat tgatagatag
     1261 atattagcgc agctaaaaaa attatgataa tgaaaacatt attaaaatac accatttttt
     1321 tctttttttc ctcttcactt tgtgaaaagt ctgcaggaaa aattactatc aaagtaatca
     1381 gcaaatatcc aaaattataa atagatggaa ttaaaaaatt ttctctgtta atgattatag
     1441 ttaagataaa actaatcatt gctccaatca gtagccaaga aaaacgcatt ttaaaaaaat
     1501 ttttctttat aaaaacctca tacaaaacta taattccacc ccaagcaaaa gctattttca
     1561 tcgggatatt agttacatta aaagtactag gcataaaata aagcagacaa atgattaaat
     1621 ttattactcg aaacgcatct cgacttaata aactcttctc taaaacaggt actattctat
     1681 tcatctatat ttccaacttt caaaatttct ttatcaattc tatttaatag tttatcagag
     1741 taatccgatt taacttttct tacaaataat ttttcacttt cttttatttg atctatatcc
     1801 ctttcatcaa accatttgtt catggaagga tcaataatat gaatatttgc taatttcgaa
     1861 gtttgtaaag tagtttcttc aatatttttc aattcaagtg gtaaatctgg tcctccaata
     1921 acatttttat ttttgaacga ggaattaaaa aaaagtgtat gaaaaaattt ttcatctggg
     1981 gcaaacatac ctattttaaa attcaggtag tcttgttgat tttgttcata catttgtatt
     2041 aattctgtta ggcattcttt cgacaaagcc caccattgag aaccatgata caaatcaaat
     2101 ttatggtgtc taatttttgg gggaagttta atcatcctca gacaacgatt aattatagct
     2161 ctaaaaattt taaatgcagt tgtgttattc ctattaattc taggataatc atgtttttga
     2221 tatacatcta tatgtttata ataaaattct ttatgtttaa tttgatctag atctattcca
     2281 cgaataaact ccactgaact attcttttta aaataattat aaatatactc attatctttg
     2341 attggataat cagcaccaga taataataca tattttaaat atttctcatt ttgtaatgct
     2401 ctctttacta aattgatttc tgcttgtaca atggaaaaac caccccaata aattttaaca
     2461 cggtttttat tacataaaaa ttcaacatta tcatatgatt taaatgcttc aataaatggt
     2521 tctatatcag tttttttatc tatatgaata ataaaaactg cattttcgtt caatcgattg
     2581 accaatctct ttaagttgcc aatatcattg tatgccaata tcaaataaca tattttcaaa
     2641 aataatcact cctaaaatat cttgaacatt tttaatgatc tctactatat atactctcca
     2701 tttgttcagt agtgtacgaa acatcataac tctttagttc ttgtgttatt ttattagcat
     2761 acggctcttt tattactttt tttgcccatt cttgtggtgt ttcatctaat gatacaaaat
     2821 ccacattatt tgttaacttt gtatctttgg aaatagtatc tgctaagact aattttaatt
     2881 ttgctgcttg tgcttctaat ccaaccattg gcagaccttc ataaagagaa ggcattacaa
     2941 aaacatccat cacagataat aactcgggaa tatcgtttct ttctcctaaa aattttactc
     3001 ttttctctat atccaattct actgctttgg ctttcagttt atttctaagt gacccttcac
     3061 ctactaaaat caagtaagaa tcagaattca tttttagtat ttctaaaaaa atctctaaca
     3121 aataagattg atttttttgt ttctctaatc gtccaatgtt tcccacaaca aaagtactca
     3181 ttgagatatc caattctttt ctcttattaa ccttagcttc tgaattttta ctataaagct
     3241 ctaaatcaat accattatga actatctcaa agggatggtt ttgaaacaga ctttcaccag
     3301 cttcttgact aacggcaagg tagtctgttg cattttgtaa caacaatttg gttaaaaatt
     3361 tacgcaatac attcattaca atattttgag attttccatc ttcagttgca tggctatgag
     3421 caattctctg tctgattcca ttctttttgg ctaaccataa attaattccc ccgccaaaat
     3481 aaacctgatt gtgaac
//
LOCUS       DAYYFU010000138         3272 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000138 DAYYFU010000000
VERSION     DAYYFU010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.144"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(489..1103)
                     /locus_tag="ACHA34_002857"
     CDS             complement(489..1103)
                     /locus_tag="ACHA34_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8793423.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1709..2086)
                     /locus_tag="ACHA34_002858"
     CDS             complement(1709..2086)
                     /locus_tag="ACHA34_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793424.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2386..3267)
                     /gene="galU"
                     /locus_tag="ACHA34_002859"
     CDS             complement(2386..3267)
                     /gene="galU"
                     /locus_tag="ACHA34_002859"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8793425.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         1002 a          669 c          500 g         1101 t
ORIGIN      
        1 gcaaaaaaaa taataaacac ttataaatat ccattatcta tccttcaatt tttgtgtatt
       61 ttatttctat tagaaaaagg tatcctgttg gacaattttg ttatatcagg taaacttcat
      121 tgcaataatg ataacaaatg tactataatt actttgtaaa gagttttggg gaagactctt
      181 tcatcaacgt gtattaagag acaagaaaga atccatatgg attctttctt gtctcttttt
      241 ccactacttt ttatttttct gacactacat attcttataa ctcatttctc ataactgtga
      301 aacaacttat aaaaataaca aactttaaac aaaacaagct taaaatcaaa gatgatccat
      361 cttaaaataa ctaaaacaaa aaaagtagtc gttgttaatc ctcattaata atcgttttct
      421 ttttttcaaa cgctccattt ttatgtggaa cattattttt ttaaggaact ttgtcaacat
      481 gtggataact attttccact tgtgtcctgg actagctgtt ttgctgtaaa atacaaaact
      541 tgctgcagca aaaagatcgt tgcaagagag gctgttccgc agatcgctgg atctagattt
      601 ggcaaagtca agactgcatc atgtaaattg gcgtggttgt ccaaaaccaa atcagcaaat
      661 tcaaataatc gcaaaccact gctatgttgc gatttgatac ttcgtgacaa atcaaatcca
      721 gtaacaatga ttaatggatg accattttct ttgatccact gagctagttc gatgattgcc
      781 gggtttcttc cttcattcga aagaagaaga aaaatgtctt ctgatttgac agaaagctga
      841 tcaataattg cctgtcctgc accttctagc gtttcataga cacctccggc tggattttca
      901 atcaatatac ttggtacaag atccggggtt tgatcccaaa attcaaatgc tacaccactt
      961 agtgtgcggc tagcatatat ctgcaatcta ccaccttgat taatttgttt cgcgatccaa
     1021 gtgccagctt gaagcatcag cggctcttct atccttaatt tctcttccaa ttggtgataa
     1081 tagtcaaaat aacataaact catttatctc tcccgcctag ctatacttga catgaataga
     1141 aacagctgcc taatctaagg aagtacataa aaaaacagta gttagacagt aacacatgtg
     1201 gtctttgctg tttcaaacac aaaaaaggca ttgaacgaat acgcccaata ccttgacata
     1261 gcaaataaaa caacgtgcag accatggaaa atatctacat aggggtagaa ttttcaccct
     1321 agattaggta caataaatta ggtcgatgca ctgcattgac tatggcaaaa tgatcggtca
     1381 gggtgtatgt gtgtgactgg tcattccctg attcttagcg ccaactaagg gtcagggagc
     1441 ctttttttat tcagctgttt ctatctgaat ttattgtaag taatctctta tccaagtgca
     1501 agcaagaaaa tacaaaaaaa cattggtatg atttatatat atataaataa cataaaaaaa
     1561 ggaatggctt atcccgatac aacaagcatt cctttaaaaa agattctttt atataagtga
     1621 aaaaagcttt tttccaccaa ttaaataaat ctctcatctc aatagtttat ccattatttt
     1681 atcttttttt gcgtatacct actaaatatt accgtcttct tctcgttctt ctactttgtt
     1741 cgagagaatc tgtactagaa aaagtagttg tatgtctacc ttttctgcct atccgagact
     1801 ttcttttcat atatgggata taaatgatta gatacagtac agcaaaaaat aaagcaaata
     1861 cgcaagcttc ccaactacct aacgccaatt caaccatcaa ggtagcaacc ccaacggatg
     1921 ctgcatacca gataaaaatc agaaacgttt ctttttttct gaccatgcga aacatgataa
     1981 atagactaat aagcaatagt ggcaacacaa atatccaatt gcttccttcc atcagccact
     2041 tgagtatatc aagcagcgac tgtgcaacag cgactaattt tcccatagtg aacacacacc
     2101 tttttttata attaaaaaat tcgccttttt attctactat gaatcttctg gcttggcttt
     2161 catttctctt atgtaatcat tttcttttat acaacttaaa aaaaaattaa accattagca
     2221 taatattttc tgaatctatt cacgaaagtc tttttctatc tttcgttttc cacgactcaa
     2281 ctgatctaga gtcaaaaaac taaaacgaca gttgcgcaaa tgacgcacaa gctgccgctt
     2341 taataacaaa tagcctttca ttctcagcag gccacttatt tccagttaac tttctgttct
     2401 tagcttttta ctcaactgga taatatatgc acttaatgaa gtcccaattt ccgaatgttg
     2461 aagtccatat tcaatatttg tcttcatata tcctaactta tcgcctacgt cgtatctttt
     2521 tcctttaaac tctctcgcat agaccgcctc tgtctggtta agagtctgaa gtgcatccgt
     2581 tagctgtatc tcgtttcctg ctcctggttc ttgagtttct aatatctcaa atattttagg
     2641 agtcagtaga taacgtccga taatggctaa attacttggt gcttttccca tttcaggttt
     2701 ttctacaaac ccttttactc tgcaacgacc cttttcgcta tctgttttag gatcaataat
     2761 tccgtattta gatgtttcat tttctggtac acgcatgacg gcaacagcgg aagcttgtgt
     2821 ttcttcataa tccatcatca attgttgagt caatgggata tcatctttca tcaaatcatc
     2881 ccccagcatg acgacaaacg gttcatttcc tataaatgct tttgcttgaa gcacggcatc
     2941 ccccagtcct cttggatgag attgacggat gaagtgaaga ttgacttgag tcgtttcttc
     3001 aactaaagcg agtaattctg ttttttcctt ttcccgcaaa ttgttttcca attcgatatt
     3061 tgcatcaaag tgatcttcaa tgggtctttt ttcttttccc gttacgatca agatgtcttc
     3121 gattcccgaa tccaatgctt cttccacaat aaactggatc gttggtttat ccacgattgg
     3181 aagcatttct tttgccatgg cttttgttgc cggcaaaaaa cgtgttccta gcccagcggc
     3241 aggaatgact gcttttttga ccttcacttg ct
//
LOCUS       DAYYFU010000139         3227 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000139 DAYYFU010000000
VERSION     DAYYFU010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.145"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..698
                     /locus_tag="ACHA34_002860"
     CDS             <1..698
                     /locus_tag="ACHA34_002860"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8793426.1"
                     /translation="SNCQAVKQLAQKHALDIIAFTPESIIYPYNIAAPETMQWEKSKA
                     YFRNAILATAELGCSLMTINSGYGLMEEPIEESWKRSREMLRYLAEIAETEGVTIALE
                     TLRPEESKIVTKLQEAQRMVHDVAHPSLKLMVDTAAMGVAGEGLQDWFKAFGDDIVHL
                     HFVDGTPEGHLAWGDGNFNLHEFLTILEKYDYQGFLGQEITDSRYLKRPYLADKQVFD
                     KLSEALRKREGNV"
     gene            695..1483
                     /locus_tag="ACHA34_002861"
     CDS             695..1483
                     /locus_tag="ACHA34_002861"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM8793427.1"
                     /translation="MKDFVAVGYACMDIYDDLGKKYPTGNGVDVIAHLSKAGYDVALV
                     SAVGDDTYGKEFIEFCEDMEFDTSHVHVEEGDTAQIHLSLIDGDRVHGKVIDGVLANF
                     SLTKEDRAFIKEFKYLHTDYFGRIYGMLGEFQQAGCKIVFDFSSFINDKDFEMVAPYV
                     DIGQISVEGFSQETKKIMQYMHCLGIKICIATAGSQGALAYDGKNFYCQEALDPQEIV
                     NTVGCGDAFLAGFLMGDAASKPIEECLLLGAKSSQKIIQKFEPY"
     gene            1504..2367
                     /locus_tag="ACHA34_002862"
     CDS             1504..2367
                     /locus_tag="ACHA34_002862"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFM8793428.1"
                     /translation="MTIDTCFHPIYFDMIQTSEAELKKREEAFGVHKQSPYTMDEILS
                     EMDYGKIEKSILHPIALTTTENVSIVTNEEISKIIQQEPNRFIGFASVDPNNPEAETE
                     LEQAFQKGLAGLTFHPGKQQIYPNDPKMKPLYQLCEKYDKPIYFHSGLSWEPRSYTKF
                     CQPLFVEDVLIEHPALRICLAHFSWPWIQEVMMLMIKYSNLYTDTSMLYLNSFDDFFG
                     DFFSNQLTPSTIERNFPRQIMFGSNTPRFRAFKVKRALEKIPFSKEVRENILTNNAYD
                     YVYGRKSRHDL"
     gene            2357..2785
                     /locus_tag="ACHA34_002863"
     CDS             2357..2785
                     /locus_tag="ACHA34_002863"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFM8793429.1"
                     /translation="MIYKEYLEFFSTEDVQLINITEEVEKVMARSNITSGVAFILSNH
                     TTTGITINEPLECVEKDIKNTLERLIPENSNYIHAHFLPSYGATSNNSPGHLKSSLLG
                     NHAMFSVEDGKLEKGSAQDIYLVECDGPQTRKILIKILGE"
     gene            complement(3084..>3227)
                     /locus_tag="ACHA34_002864"
     CDS             complement(3084..>3227)
                     /locus_tag="ACHA34_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8793430.1"
                     /translation="DFREVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYS
                     SLI"
BASE COUNT         1043 a          546 c          683 g          955 t
ORIGIN      
        1 attctaattg ccaagccgtt aagcaactag cacagaaaca tgctcttgat atcattgctt
       61 ttactccaga atcaattatc tacccttata acattgctgc gccagagacg atgcagtggg
      121 aaaaaagcaa ggcctatttt cgaaatgcaa tattagctac ggctgagtta ggctgctcac
      181 tgatgacaat caactccgga tatggtttga tggaagagcc tatcgaagaa agctggaagc
      241 gctcaagaga gatgctacga tacctcgctg aaatagcaga aactgaggga gtcacgattg
      301 cattggaaac gttgcgtccc gaagagtcga aaattgtaac caaattacag gaggctcagc
      361 gaatggttca tgatgtggca cacccatctt tgaagctgat ggttgatacc gcagcaatgg
      421 gggtcgctgg agaaggtctt caagattggt ttaaagcctt cggggatgat attgtccacc
      481 ttcattttgt tgatggaaca ccagaaggac atcttgcctg gggagacgga aattttaatt
      541 tacatgagtt tcttactatt ttagaaaaat atgattatca gggatttctg ggacaagaaa
      601 tcacagatag ccgctatcta aaacggcctt atcttgcaga caaacaagta tttgataaat
      661 tatcagaagc attgaggaaa agagagggaa atgtatgaag gattttgttg ctgtaggcta
      721 tgcatgtatg gatatctatg acgatttagg gaagaaatac cccactggaa atggagtgga
      781 tgttatagct catctctcaa aagctggtta cgatgtagct cttgtttcag cggtaggaga
      841 cgacacttat ggtaaagaat tcattgaatt ttgcgaggat atggaatttg atacttctca
      901 tgttcatgta gaggagggag atacagcaca aatacacttg tctctgatag atggggatcg
      961 agttcatgga aaagtaattg atggtgtact agcaaatttt tccttaacaa aagaggaccg
     1021 ggcattcatc aaagagttca agtatctaca cacagattat tttggccgca tctatgggat
     1081 gctaggagag tttcaacaag ccggctgtaa aattgtattt gatttttctt cttttatcaa
     1141 tgataaggat tttgaaatgg ttgctcccta tgttgatatt ggacagattt ctgtagaagg
     1201 attctctcag gaaactaaaa aaattatgca gtatatgcat tgtttaggaa tcaaaatctg
     1261 cattgccact gctggaagtc aaggggctct cgcctacgat ggaaagaatt tttattgtca
     1321 agaagcgctt gatcctcaag aaattgtcaa tacagtgggg tgtggggatg ctttcctcgc
     1381 gggtttccta atgggagatg cagctagcaa acctattgaa gaatgtttgt tattgggagc
     1441 taaaagttca caaaagatca ttcagaaatt tgaaccttat taattgacag aggagcggaa
     1501 gtaatgacca tagatacttg ttttcatcct atctatttcg atatgattca aacatcagaa
     1561 gcagaattaa aaaaaagaga agaggctttc ggtgtacaca aacaaagccc ctatacaatg
     1621 gacgaaattc tttcggagat ggattatgga aagatagaaa aatcaattct tcatccaatt
     1681 gctctaacga ctaccgaaaa tgtcagcatt gtgacaaatg aggagattag caaaattatc
     1741 caacaggagc caaatcgttt tattggtttt gccagtgtag atcccaataa tcccgaggca
     1801 gagaccgaac tggaacaagc ctttcaaaag ggacttgccg gtttaacttt ccatcctggg
     1861 aaacagcaaa tttatccgaa tgatcctaaa atgaaaccgc tttaccagtt atgtgaaaaa
     1921 tatgacaagc caatttattt tcactctggt cttagctggg agccaagaag ctacacaaag
     1981 ttctgtcagc cactatttgt ggaagatgta ttgattgaac atcctgcgct tcgaatctgt
     2041 ttagcacatt tttcatggcc ctggattcaa gaagtgatga tgttgatgat aaaatattca
     2101 aatttgtata ctgatacctc tatgctttat ttgaactctt ttgatgactt ctttggagat
     2161 ttttttagca atcaattgac tccctcaaca attgagcgaa attttccaag gcaaattatg
     2221 tttggatcaa atacaccacg ttttcgagcc ttcaaagtga aacgggctct ggaaaagatt
     2281 cccttctcaa aagaagtgag agaaaacatc ttgacaaata acgcttatga ttatgtatat
     2341 gggaggaaaa gtaggcatga tctataaaga atacttagaa ttcttttcca cagaggatgt
     2401 ccagttaata aatattactg aagaggtaga aaaggttatg gcgcggagca atataacaag
     2461 tggcgttgcc tttatcctta gtaaccatac aacaacgggc atcacaatca atgagccact
     2521 ggaatgcgtg gagaaggaca taaagaatac actagaacga ttaatccctg agaattcgaa
     2581 ctatattcat gcacattttc ttccctcata tggagcgaca agtaataact ctccaggaca
     2641 tttaaaatcc tccttactag gaaatcatgc aatgttttca gtggaggatg gtaagctgga
     2701 gaaggggagt gcccaagata tttatttagt agaatgtgat ggacctcaaa ctcgcaaaat
     2761 tctaataaaa atactaggag agtagttttg aattaccgga agggactggg acaaaagttt
     2821 tttcttaaaa aatagtccca tttcttcagt attaattaaa taaaaagtaa aatacattcc
     2881 cgaaactatt ggatgaagat cccagtagtt tcgggaatgt attttttcta gaggctttct
     2941 tttgctcttt gtcaagaagt gttgatgacg agattttcta attcaaatga aggataattt
     3001 cacaatctat taagcagtgg ctaggaacca attggttcct aagttgctgc ttttatttct
     3061 aataatttat attctatgat ttgtcaaatt aagctagaat aaaatgcttc ttgtacatag
     3121 ctcaaaaata tttcgattgg tgttctgtaa ttcaatgatt ttcttggaat atgattacgt
     3181 tgattgctga cactggaaat aaatgtctga ttcacttctc taaaatc
//
LOCUS       DAYYFU010000140         3201 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000140 DAYYFU010000000
VERSION     DAYYFU010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.146"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..714)
                     /locus_tag="ACHA34_002865"
                     /pseudo
     CDS             complement(34..714)
                     /locus_tag="ACHA34_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            complement(1036..1257)
                     /locus_tag="ACHA34_002866"
     CDS             complement(1036..1257)
                     /locus_tag="ACHA34_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793431.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(1355..1600)
                     /locus_tag="ACHA34_002867"
     CDS             complement(1355..1600)
                     /locus_tag="ACHA34_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793432.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1840..2454
                     /locus_tag="ACHA34_002868"
                     /pseudo
     CDS             1840..2454
                     /locus_tag="ACHA34_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2824..>3201)
                     /locus_tag="ACHA34_002869"
     CDS             complement(2824..>3201)
                     /locus_tag="ACHA34_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFM8793433.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT         1037 a          583 c          466 g         1115 t
ORIGIN      
        1 aatcaaacgc gccctctctg aactccaaat atcttaggct gttattccca ttaatacctt
       61 gatttcagta gacaccgaaa agccgaagag cgttccattt cttcggttct ttttatatat
      121 tcctcgaatg gtctccatgc ccttaatcgt ggaagaggct gtacggagac tttgataaaa
      181 tttatttcgt cgtttaatag gtcgatggtc ttgttctatt aaattgttaa gatacttcac
      241 agttcggtgc tctgtcttag tatataaacc cacactctgt aactttctaa aggcggagcc
      301 aagagaaggt gctttatcgg tcacaattgc tttcggctca ccaaactgtt tatggagtcg
      361 ttttaagaaa gcataggctg cttgcgtatc ccgtttcttt cgtaaccaga tatctaaggt
      421 taagccgtcc gcatcaattg cacgataaag ataatgccaa cgtcccttaa ttttgatata
      481 ggtttcgtcc attttccatg aatagaagga ttgtctattt ttcttcttcc aaagataata
      541 gaggactttg ctgtactctt atacccaacg ataaatcgta gtatgacaaa catttattcc
      601 acgatcatat aacaattcct gaacttcacg atagcttaga ttgtaacgca ggtagtaacc
      661 aacagcgaca ataatgacgt cttttttgaa ttgtttgcct ttaaaatgat tcattactct
      721 gtcctctctg tcttttttct caattttaca ctaaaataga ttttttggaa aactttgcaa
      781 cagaaccgtt tttactatat acttcttccc agaacttgtc cgtctccttc aaattatctc
      841 tacctctcga gttcattggg tggtttgccc atttattaaa catggaatca cctttctcat
      901 actcatatat ttatgtccta tttcaattat atttatatat gcttaaataa atttgtatcc
      961 tgaaatttat tgtaatatga agctgtattt tacatatagt aaccaaagag ttaataaaat
     1021 aaaaagagcg aaccattata gatcagctct tttttcattg ctgtttttaa tcttatctag
     1081 aatcacttca aatgtttcaa aggtatttac atctaactga cataaccaaa gcaagtactt
     1141 cttttctttg taagtccaat caatattacg gtaagaggac attagattat ggtaccttgc
     1201 tctttgttca actgaagcca attcccagcg tttttcaaat attttttctt cttccattat
     1261 tgatccctcg caagacgcta cctcaaactt aaataatggg cagcgaaagt ctaattcctt
     1321 agatctaagt atagcaaaca aatatgaaaa aaacttagaa ggtagtaaaa gttcttccgt
     1381 cttttgcttt gcttctattc ttaatgtgtt tcgttatcgg tgccctacaa agattttcat
     1441 taattacaca ctcatttatt gttgtgtatt ttctgacatt tctctcttga tcaattattt
     1501 taattccatg ggcatcctga tagttcatag gagaggagcg gctatcagaa atagttgcac
     1561 ctatctcttg aacttctttt acaacttctg gaaaatccat ccatcctgct tcagctgtca
     1621 aaagtaataa tctataatat ttatctttcc ttttcaatct aagaaatcgt caccccattc
     1681 tttttcttct attataaact atttaacttt tagaattcaa aaaccgtttg tctatttaga
     1741 gtttacccta aatagacaaa aatataacat tgaaaaattt agagttctaa cgtatgatta
     1801 gggtgtatta taaacgttat agaaaagtag ggaataaatt tgaaaattgc ttatattaga
     1861 gtttcatcca tcgatcaaaa tgaacaacgt caaattgaag aaatgaaaaa gtttggcgct
     1921 gaaaaaattt ttatcgaaaa acaatctgga gcgacgatta gtcatcggcc gattttccag
     1981 gaagctttag attttgtcag agagcaagat atttttattg tagaagccat tgatcgactt
     2041 ggacgcaatt atgacgagat tatcgattca gtgaattatc taaagaagaa gaatgtgcga
     2101 cttattatca ccagtttacc gatcatggct gaagcaattg gaaatcctct cctagataaa
     2161 ttcatcaaag acttaatcat ttaaatctca gccatgattg ctgaacaaga acgtactgaa
     2221 tcgaaacgac gccaggcaca aggaattaaa atcgctaaag caaacggcgt ctataaaggt
     2281 cgtcctaagt tatacagtgc agacaccaaa gatcctcaac gtcgccttgt ctataggagt
     2341 atcgttcagg atcttgagaa tggggttgct atctcaaaga ttgcaacaga ctacaatgtg
     2401 actaggcaaa caatttaccg aataaaaaaa gaaatcgacc aactaattgt atgatttctt
     2461 ttttgaatca cgtaattatt aattagcttt gtcatcaata cagacaatca ttttagattc
     2521 atctagctaa gttgattaca tttatgttaa aaagaattta gttaacataa atataattga
     2581 tgcataaatc gtccttattt aaaggcattt aacattgaca acttagtata atgtatctta
     2641 tgtaaactaa taagatttga attttatttt atacacactc gataatagat caattttatg
     2701 gtcccccgca tttacgattg cacaagagaa acataaagtc gacctattag catttctttt
     2761 caattcaaac attaaaaaag tttatacctc tgctactaaa aaatgaaatt atctatttaa
     2821 aatttaaaac ggtaaatctg attcatccaa attggaaaat ggatcagcct gtattggagc
     2881 tttgtatttc tccataataa cagatatctt tttttcattt tttaaaaaat accttttaaa
     2941 tttcttatag tcctctgaaa tcaaaatata gcgtgatgga tactcatcaa aagaagaatc
     3001 gtattcttcc tttaaaattc ccgatttact taatttttct agtacatcaa atactctatc
     3061 actcaagtaa ctccttaaat tgttatcact ttcccattga actacttttc tataagttga
     3121 attatcagtt gaaaagatag aattttcatc cataaacgca tatattagca gcaatctttc
     3181 atcattgtca aattctattg g
//
LOCUS       DAYYFU010000141         3199 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000141 DAYYFU010000000
VERSION     DAYYFU010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.147"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACHA34_002870"
     CDS             301..585
                     /locus_tag="ACHA34_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793434.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACHA34_002871"
     CDS             588..1004
                     /locus_tag="ACHA34_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793435.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACHA34_002872"
     CDS             complement(1141..1860)
                     /locus_tag="ACHA34_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8793436.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2522..2608
                     /locus_tag="ACHA34_002873"
     CDS             2522..2608
                     /locus_tag="ACHA34_002873"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8793437.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2910..>3199)
                     /locus_tag="ACHA34_002874"
     CDS             complement(2910..>3199)
                     /locus_tag="ACHA34_002874"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793438.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1038 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt atttcattgg tccaagctaa aaaactctta
     2881 gaagcgattt tagagccatt taaacccact taattatatt ctccatgaaa ttcgctccat
     2941 gcgtcaaaag ctgcgtctga aattccgccg tcttcgttca ctagttgcat taatttttcc
     3001 aacgatcgtt ttctttcggg tgtcatattt ttatattcca acaatttttc gtctgttaaa
     3061 tcgaacttga ttgcttgaat tgcttgctct aatttttcta agttttgaaa atattcgtaa
     3121 ggaaattttt ccattttttc tttctccagt ctcacaattt ttctagaaat aaaatcactt
     3181 ctttgccgtc tatactctc
//
LOCUS       DAYYFU010000142         3012 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000142 DAYYFU010000000
VERSION     DAYYFU010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.148"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            177..359
                     /locus_tag="ACHA34_002875"
     CDS             177..359
                     /locus_tag="ACHA34_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8793439.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="ACHA34_002876"
     CDS             462..746
                     /locus_tag="ACHA34_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793440.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="ACHA34_002877"
     CDS             762..998
                     /locus_tag="ACHA34_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793441.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="ACHA34_002878"
     CDS             1020..1274
                     /locus_tag="ACHA34_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793442.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="ACHA34_002879"
     CDS             1356..1541
                     /locus_tag="ACHA34_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFM8793443.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="ACHA34_002880"
     CDS             1608..2003
                     /locus_tag="ACHA34_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFM8793444.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            2020..2370
                     /locus_tag="ACHA34_002881"
     CDS             2020..2370
                     /locus_tag="ACHA34_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793445.1"
                     /translation="MKATHLNYFSRTLINNLLKDTTGISVSTKHFANDLIIFEDNNLR
                     IEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKKAKLLFEFQT
                     DYHNELLAQSEILD"
     gene            2392..2766
                     /locus_tag="ACHA34_002882"
     CDS             2392..2766
                     /locus_tag="ACHA34_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793446.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          924 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc tt
//
LOCUS       DAYYFU010000143         2886 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000143 DAYYFU010000000
VERSION     DAYYFU010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.149"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            798..1052
                     /locus_tag="ACHA34_002883"
                     /pseudo
     CDS             798..1052
                     /locus_tag="ACHA34_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1122..1634
                     /locus_tag="ACHA34_002884"
     CDS             1122..1634
                     /locus_tag="ACHA34_002884"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8793447.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1631..2668
                     /locus_tag="ACHA34_002885"
     CDS             1631..2668
                     /locus_tag="ACHA34_002885"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8793448.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1041 a          513 c          574 g          758 t
ORIGIN      
        1 ttgatgacca cgaaagtgca tggcgatatt tgatgttgat actttatgca gtaccctatg
       61 tattcttcgc tatcattctg tggctcatag cattgttttt tagagtattt aatgaccttt
      121 aggtagattt atatataaaa agggctaaat acgcaaaata cccccttaaa atgcaaaata
      181 aatgcaaata agggggtaac tccttatgaa aaaacaacta atagtattat aacacgtttt
      241 tatgccgaga aaacttattg gttggaatgg gctatgtgtt agctaacttg ttagcgagtt
      301 ggttggactt gaattgggat taatcccaag aaagtaccaa ctcaacaaca cataaagccc
      361 tgtaggttcc gaccaataag gaaattggaa taaagcaacg aaaggagttg agtaaaatga
      421 gtgagaaaac gaagaaaatc attcgagtat cattagcttt ttcacaagta gtagtgcaat
      481 cattgatagt ttattatact taccagcaat ataaattatc taaagagaat taagttttga
      541 ttatgtaata agttttggta ctaaaaaaga cttgatctaa ttagaccaag tcttttgtta
      601 gtgttatatt aataacaaaa taaaaataag aagtcgctca ctccccgacc aaagtttgtg
      661 agcaaaataa acagtcgatg tttgtttctt aattgtatat cttgatatta aacgatttaa
      721 gattattctt caagatatat attcgcaaat gtaattggaa aagcgacttc ttaaacaaaa
      781 ttaaggagtc gctttttatg gcaaaaataa cgaaaaatga gcaagaaaat ttggtggaaa
      841 attacacaaa aaaaccggcg tactctaata gccggttaga cgcacatttc gtgtgcactt
      901 ctaatccgaa attgagcttt gacgctatga cgatagtcgg aaaccttaat aaaaacagcg
      961 ctaaaaagct atccgatttt atgagtttag atccacaaat tagactttgg gatatacttc
     1021 aaacaaaatt taaagctaaa gcactacaag accccctatg ttaggctagt tgtcgtatag
     1081 gataataagt tataataaag actatacgga ggatgattac catgacaaat aaaaacaatg
     1141 aacgttactc taccgaactg aaagcgacag ccatcaagaa aatgatgcca cccgaaaata
     1201 gggggattcg ccaattaagt caaactttgg gcattccaga agcgactctt tattcttggc
     1261 gtaaaaaagc tagaattcaa gggaatacta cacccggaac gaaaaaaaat gtcgaaaaat
     1321 ggtcaagtga agataaattt ttaattgtga tggaatcatt ctcaatgaac caagtagaac
     1381 ttgcggaata ttgccgaaag aagggcttat ttaaagaaca aattgaggct tggaagaaaa
     1441 cgtgcttatc tgcgaatgaa caggaagaaa atcgaacgag agaacttgca actgaactaa
     1501 aagaagagaa aaaacaagcg cgtcaattag agaaagaatt acgtcataag gaaaaagcat
     1561 tagcggaagc cgctgctctc ttattactaa gaaaaaaagc acacgcgatt tggggggacc
     1621 aagaggaaga atgatttcat cttcagatcg caaacaagct gttgaactta ttcaagaagc
     1681 caatcgcaat ggggcccgtc tcacctatgc ctgtaatgag ctgaatatta gcgttcgaac
     1741 ctacgaacga tggacgcgtg aaggtacgat agcgcatgat caacgccctt tggcgaaacg
     1801 tcctgtaccc aaaaataaac tgacggatca agaacgcgaa aaaataatag aaaccgtttc
     1861 aaaaaaagaa tttatgaatt tgccacctag ccaaattgta ccaaaacttg cggattgctc
     1921 gatttatatt gcatcagagt ctagcttcta tcgtgtattg cgagaaaaaa atatgcaaca
     1981 tcaccgtggc cgaagtcaag tttcacaaaa acgcataccg ccaagtcacc ttgcgacgaa
     2041 gccaaatgaa gtttggactt gggatattac ttggctaaaa ggtccaataa aaggactctt
     2101 ttaccggtta tatctcatca ttgatttatt tagccgtaaa atggtaggct gggagatttg
     2161 ggaatcagaa gaagctcgtc atgccgaaaa acttattaaa cagacggtgc ttaaggaacg
     2221 tatccaagga cagccgttgg ttttacactc cgataatggc agccctatga aagccgcgac
     2281 ttttcaagtc ttactcgaaa aattaggaat tcaaagttct tattcaagac cacgagtgag
     2341 taatgacaat ccttattcgg aagcggcttt ccgaacgtta aaatatcgac cagaatatcc
     2401 caataacggc tttaaaacaa tagatgatgc tcgaaaatgg acgaaagagt ttgtcgattg
     2461 gtataacgat tctcatttgc atagtgggat tcaatttgtg tctcctgcag cgtgtcatga
     2521 aggacgacat ctcgctattt tagagaaacg aaagcaggtt tacgaggacg ctaaaaagcg
     2581 ccatcctgaa cgctggtcaa aaggtgttcg aaattggact ccacatcaat cggtggcgtt
     2641 gaatccttta aaagaaacag aaagttaaca ggagcataat ctttcaagat tatcaaataa
     2701 ccctcgtcac ccaatagggg aaaaagttat tcttccccta ttggataacg aggccaaccg
     2761 accataggga ggttctgctt tttgataggt ggaaaataaa aatacgacaa ctatattgac
     2821 aaacaccgac catcgtccag taaagagacg caataaattc tatcgaagtt tacgcactgc
     2881 ctctac
//
LOCUS       DAYYFU010000144         2751 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000144 DAYYFU010000000
VERSION     DAYYFU010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.150"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..418
                     /locus_tag="ACHA34_002886"
     CDS             <1..418
                     /locus_tag="ACHA34_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8793449.1"
                     /translation="AIGEFVHYLEKELTIEVNDLQKELIFNFSISKIVENLTILDPDK
                     VLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKESVDEQEDIEEYMQAN
                     GEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            431..1063
                     /locus_tag="ACHA34_002887"
     CDS             431..1063
                     /locus_tag="ACHA34_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8793450.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            1962..>2751
                     /locus_tag="ACHA34_002888"
     CDS             1962..>2751
                     /locus_tag="ACHA34_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8793451.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWL"
BASE COUNT          914 a          417 c          546 g          874 t
ORIGIN      
        1 agcaattggc gaatttgttc attacttgga aaaagagctg actattgaag tgaacgattt
       61 acaaaaagaa ttaattttca atttttcaat tagtaaaatt gtcgaaaatt taaccatttt
      121 ggatccagat aaggtgctat cttttgtaca aaaagcaatt aaagaattat cgaagttatt
      181 aaattttgat ttttctaata atcagctgtt tttgttgtac cttcactgta gttgtatgat
      241 tgaacgaatt gtacgtaaag aaagtgtgga tgagcaagag gacattgaag aatatatgca
      301 agcaaatgga gaaataatgg ataaaattca tagcgctttt tttgagattg aaagggaata
      361 cacaattgaa ataccagatt tagagttgcg attattgagc gatattgttc aaggatagga
      421 gtgaattcga atgacaatgg gagtaatttt tgatatggat ggggtactca tagacagcga
      481 gacattttat tttgagagaa gaatgaactt ctttcaagaa ctggagatag atccaggatc
      541 aaaccagatt gatgattatg tggggaaaac agaagctgca atatgggaaa cattagttcc
      601 tgaggacaaa tcattaagag aggaacttta tccgaaatat gtagattatc gaaaaacgca
      661 tccaattgat ttcgctaaag atttacgaaa agaagtgaaa ccgttacttg acttcctcaa
      721 gaagcgggat ataccattgg cactggcatc ttcttctcca gttgttgaga tagagagtat
      781 gctcattcaa tgtgaactta ctgaatattt ttcctttgtt ataagtggtg agtcattaaa
      841 acaaagcaaa ccacatcccg aaatatattt aaaagcgaaa cagcaattag actgtgactt
      901 ctttattgca gtagaggatt caacgattgg tattaaatca gcaaaggcag caaatatcta
      961 tacggtggca ctaaaacaac caatgcaaat cgatcagagt gaagcagaca tacaaattga
     1021 aagtctacaa gaactggaac aaattgtttt agaaaaaggt tagtgaatta atggaaataa
     1081 ggctatatat ctgtgctagc tattttgact aaccaaaaaa acttgctata attcaggtgt
     1141 agtaaaaggt gctggtaaca ccttttacga aaatgcttcg gcagtgataa tgggattggc
     1201 tttttaagtt ttttattttg ctcgcaatcc ctaaggtcat gtgaatgatc ctagggattg
     1261 ctagaatatt ttgcaacatt ctatctattt cagaacgtaa ccttccccag ttcattggga
     1321 ttcattagca tacaaccaac atttcaaaaa gaaattgcac tacaccatta ttagctattt
     1381 tgactaaccc caaaaaactt gctataattc aggtgtaggt aaaaggtgct ggtatcacct
     1441 tttacgaaac tgcttcggca gtggtaatga gtgtggctta aaggactagt cctttaagct
     1501 ttttactttg ttcgcaatct ctaagatcaa gttaatgatc gataagattg ctagaatatt
     1561 ttgcaacatt ctatttcaga acgtaacctt tcccattcca ctgtgattca ttggcaaccc
     1621 tcctcacaac caacgtttca aaagaaattg taaagttgtt ggggtaccac gaccgaacaa
     1681 tactacacct gaatgatagt aagttgtctc attaagtata gcatatcctc tttttgggta
     1741 aatagaaatc ggttacattt tttatccaag accaagtttt ttcatttggt cttatttgct
     1801 atacccaaac aattccgcag tatagtcgac ggtattgatt ttatcaggtg agtaatagaa
     1861 acttgttttc cttccccaaa acattaccta taaaggagaa aaaatggtat cgtaattata
     1921 taggtatagg atgaaatttt tttctaagag gagggttgtt gatgaaaaat ttacagttaa
     1981 aatttattgt tgatccatcg attgtacgct ggtttcggat tatcaatcaa tttgagcgca
     2041 atcaacttgc cacaaaagga gaattagcaa agagtaatca tgtatctgaa cgaaccattc
     2101 aaacagatgt caataaaatg aaggactact tttctgaaag tgttcaattt gtttcaacga
     2161 aaagtgggta ttcttttcag aaggaaagac cggttctttt tctcaagcag aaggagcaac
     2221 tactggagaa cgaaatttta tttgagttgt tagggaatgt cttttatggc gaattggaag
     2281 atatgacgga gctaattgat cgttttcatt tatctgaagc gacttttcgt cggtatttga
     2341 agcagattca accagttttg gaagaatacg gcttagaatt gagtctttat cctcttgatt
     2401 taaaaggaga agaggcaaat attcgcaagt tttttaaaga cttctattat gagggggaga
     2461 tgacgcccca tactcttgtt ccaccaaggg atcttcatga acttgtgcaa gctacattct
     2521 atgataaatt tgagtatttc tcgattggaa cagggacttc tcctgctgaa ttttattata
     2581 gtttgtatat tgcgattgag cgtgtgagac aaggaaaaac gatagccatt cctcaaggtc
     2641 tgattgagcg agtaattgct agtgagaatt ttcgcttaat gtattccttg aaagaagcga
     2701 ttcaaacaaa ttatcaagtt gctttatcag aagaggagtt ttgttggttg t
//
LOCUS       DAYYFU010000145         2739 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000145 DAYYFU010000000
VERSION     DAYYFU010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.151"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="ACHA34_002889"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="ACHA34_002889"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8793452.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1072 a          425 c          558 g          684 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatata
//
LOCUS       DAYYFU010000146         2488 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000146 DAYYFU010000000
VERSION     DAYYFU010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.152"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..445
                     /locus_tag="ACHA34_002890"
     CDS             191..445
                     /locus_tag="ACHA34_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8793453.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="ACHA34_002891"
     CDS             446..763
                     /locus_tag="ACHA34_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8793454.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1322..2257
                     /locus_tag="ACHA34_002892"
     CDS             1322..2257
                     /locus_tag="ACHA34_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793455.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILITRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
     gene            2351..>2488
                     /locus_tag="ACHA34_002893"
     CDS             2351..>2488
                     /locus_tag="ACHA34_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFM8793456.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
BASE COUNT          921 a          342 c          457 g          768 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga aacgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acatccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattactac
     1381 tttcaaaaga ttatccgagt taatgcagca gactgttaaa agtgctatgc ctgaaggata
     1441 caagtttaat tttgttcaat cttcagtatc tactgaaagt atctattttt caattctttc
     1501 tgagagtagg ctttttcatt ttagaataag ttctcatgta aagaaaactg atgttgatat
     1561 atatacgttc cgtacaaata attataaaaa tttaaatgat ttgagtagaa gtatcagtga
     1621 atatctcaag gggaatagct atctcaagat tcgattttca catatattaa ttttactacc
     1681 atatttgaat gagaatttta ccctaattca agtagataag tctttcattg cctcaggtaa
     1741 tcaaggaaat atgaatatta caagtaacaa aacgtcacgt gtgctattgg cgttgatggg
     1801 ttacggaatt ctttatagag atgaccaaaa tggccagatt ctaataactc gggctgggcg
     1861 ttcattatta atgaaatata gaaaatttat accggaagat tttcaaaact tacagttaga
     1921 aaacatgcgt ttagaagatt tatttagatt agcagaagat gataagtata gatttgaagt
     1981 tttgaaaatt caaaagaatg aactattgaa taagttatat cctgagaatg cgataaaagc
     2041 acattttggt gatgctaatc atatgtatag ttggttcatt cctaaaagat ttagagggaa
     2101 aatcaatgct ggagatcaag tgaaagtact caatcgaaat agtacagatg tcgtaatgat
     2161 aacagatatc tatgtagaag atgaagatat aatcaaatat atgaagcctg tgataaaact
     2221 tgttaataaa aataaaggaa cccgtggtgc tagttaattt tttcctagag gaaaatgtct
     2281 aaacatgtta gtaaatcaac aaaaaaacgc atatagtagt ctcaaaactc ccaagaaatg
     2341 aggactacat atgcgttcat caaaatatac tgaacattat acagatactt ttataacatt
     2401 caaggaaatt atgagaactg tttctaacat ataccacaat tgtgtaccag ataagattaa
     2461 aaaccgaaga aatactgatc aactgaag
//
LOCUS       DAYYFU010000147         2473 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000147 DAYYFU010000000
VERSION     DAYYFU010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.153"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..624
                     /locus_tag="ACHA34_002894"
     CDS             85..624
                     /locus_tag="ACHA34_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793457.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            1644..>2473
                     /locus_tag="ACHA34_002895"
     CDS             1644..>2473
                     /locus_tag="ACHA34_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8793458.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          905 a          326 c          397 g          845 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt tta
//
LOCUS       DAYYFU010000148         2450 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000148 DAYYFU010000000
VERSION     DAYYFU010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.154"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            113..505
                     /locus_tag="ACHA34_002896"
     CDS             113..505
                     /locus_tag="ACHA34_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793459.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(678..881)
                     /locus_tag="ACHA34_002897"
                     /pseudo
     CDS             complement(678..881)
                     /locus_tag="ACHA34_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            847..1044
                     /locus_tag="ACHA34_002898"
     CDS             847..1044
                     /locus_tag="ACHA34_002898"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793460.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(1087..1662)
                     /locus_tag="ACHA34_002899"
     CDS             complement(1087..1662)
                     /locus_tag="ACHA34_002899"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM8793461.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1817..2343)
                     /locus_tag="ACHA34_002900"
                     /pseudo
     CDS             complement(1817..2343)
                     /locus_tag="ACHA34_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          781 a          422 c          378 g          869 t
ORIGIN      
        1 ttaagtaaaa aatataggtg attttttttg acttcatatt tataataaag acacgtaaat
       61 caattatttt tcattttact ttaactaata atgtttattt ggagggatta ttatgttaga
      121 tgatagattg acgtgttcta attgtggtac attattttac tatgaggaag ttagaaatat
      181 tgtaaaacat agaaaaaaag aaatgcctat acgttgtcca aattgcaatg aagttattaa
      241 gcgaaagttg tctcatggtt attttgtcag ctacgaacta tatgaagaaa gtaaaataaa
      301 tctgaaaaaa aaaccaaaat actataaaaa tttgaaaact ggagctatta taactgaaaa
      361 agaatttaaa aatttagtga ctgaagaaga aagcaggttt tataatttgt ctggtaatat
      421 ttgccctgga tttatggatc agattattga aaatgagtca attgaatcaa ttgaaaacta
      481 tgttcctgtt gacttaccaa aatagaaaaa ataatcctgt ttttacagtg aattatttta
      541 attttgattt tcaataaaaa tatattacaa taataatcgt aatagctaat gaaacaattt
      601 ctcaatcttt ttaccactcc aaacagggag cgtcaataat tttgtgtaaa taactcgtcc
      661 ttctgcaaaa taatgggtta ctcagtaaac attgaagcta atgtatccgt cacttgttgg
      721 aagcctttat ggcttctatt taagaatttt tgattgtatg tatcaaagat ggatactagg
      781 aaacgctcta gtgattcttc attttgaaac tgctcttttc tgcggctgta tttcttgatt
      841 tgtttattga aagattcgat tgtagaacca gaaggaatgg agaacacttt tgaactttac
      901 aacaaaacta ctgttgataa attaattcca attttagttc caactgattt aaatggtcat
      961 gatgttttac ttcgttttaa cggattttta ggtgaggatt atagatacct aaaaatttct
     1021 gatcctcaac ctttaacaga ataataaaaa acgtgtaatc aattatttga ttacacgttt
     1081 tttatttcaa tcttcgttgt agtagtggtt gcgacatacc gaaaggtaat cttcattacc
     1141 tcctactaca atctgttcac ctgaataaac tggaatacca tcaacaatac gagcattcat
     1201 tacagccttt ttattgcaaa aatggcaaat agttttaatt tcctcaattt tatcagcaaa
     1261 tgtcaataat gcttcggatc ccttgaagag attattttga aaatcattct tcaatccata
     1321 gcaaattact ggtacttcct cattcattac gataagtgat agatcaataa tattctctct
     1381 acttaaaaat tgagactcat cgaccaaaat acaatcaact tttccgtact gatcaataat
     1441 ctccactaat tctccgtaca catccatttc agaagtaatt gcaatagctg gtttcgaaac
     1501 acctatacga ctctcaactt ttccaaatcc tgatcgagta tctattgcgc ttgtcattat
     1561 gataactctc ttcccttgtt cttcataatt atgagccact ttcagtagct caatactctt
     1621 tccactattc attgttccat atctgtaaaa taattgtgcc attaaattta ttcccttctt
     1681 ttacaattca attattcctt aattccatct gcttttatgc agctgctcgt ttattccttg
     1741 atatgtctat aacatctact atccctaaaa ccaatttata agccattaga gatggtattc
     1801 ctgaacatct ctcatattgt tttttgagtg gtatatcatc tgaaaaactg aaggaatcat
     1861 gaaaatagcg acatacttat catttagagt tctaacttta aatgcattta cttcttcagt
     1921 cagcacttta gtcatgttgg acatggtttg gggcgtgtag tgatgcccat acattttctc
     1981 aatcaagtct gcgatttccg acatggtaat accttttcgg aagaggtgaa tgacggtctc
     2041 ctctaatgtg tcattcgtcc gtctataagc aggaacagtc tgttgcttga actcgccatt
     2101 gcggtcgcgc ggaatctgga gatgaagttc cccatactcg gtcttgaccg tacggtcata
     2161 ggagccgtta cgagaattac ccgtgttaaa accaatgcga tcgtactttt cgtaatcgag
     2221 gaaagccgtt aactccgttt tgagaagcgt attgacagct ttttccaaat ggacacggaa
     2281 taatccattc aaatcgcctt tgttcgctag agtctttagg atttctgtag taaaatcgtt
     2341 catatggaag tcctcttttc tgtgaatgtg ttgtggtaac tctattctac agaagggact
     2401 tccttttttc tatttacaca aaatatttta cactctcaac aaaagtatct
//
LOCUS       DAYYFU010000149         2407 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000149 DAYYFU010000000
VERSION     DAYYFU010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.155"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1150
                     /locus_tag="ACHA34_002901"
     CDS             5..1150
                     /locus_tag="ACHA34_002901"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFM8793462.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="ACHA34_002902"
     CDS             1321..1779
                     /locus_tag="ACHA34_002902"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFM8793463.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="ACHA34_002903"
     CDS             1938..>2407
                     /locus_tag="ACHA34_002903"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFM8793464.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DAYYFU010000150         2398 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000150 DAYYFU010000000
VERSION     DAYYFU010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.156"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACHA34_002904"
     CDS             207..2069
                     /locus_tag="ACHA34_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8793465.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="ACHA34_002905"
     CDS             2069..>2398
                     /locus_tag="ACHA34_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8793466.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DAYYFU010000151         2242 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000151 DAYYFU010000000
VERSION     DAYYFU010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.157"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..321
                     /locus_tag="ACHA34_002906"
     CDS             <1..321
                     /locus_tag="ACHA34_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM8793467.1"
                     /translation="FFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKF
                     YEVNPVLEEPVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFG
                     IHYD"
     gene            389..1099
                     /locus_tag="ACHA34_002907"
     CDS             389..1099
                     /locus_tag="ACHA34_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8793468.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            1135..>2242
                     /locus_tag="ACHA34_002908"
     CDS             1135..>2242
                     /locus_tag="ACHA34_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFM8793469.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAY"
BASE COUNT          760 a          340 c          496 g          646 t
ORIGIN      
        1 ttcttttcta tagaagactt ttccgatgaa gaagtaaagc agtttttgat gaagcatttt
       61 tgtgagcgaa atgatgtatt gattgcaacc cgaggagaaa aaacggctat tggttatgat
      121 ggagcaaagt tttatgaagt gaaccccgtg ctagaagaac cagttgatac aatggcagcg
      181 ggagattctt tcttaacagc tttcctactt tattttctcg aaggggacga tatagtcacc
      241 gcgatgaaaa aggggaatga atttgctggg aagtcttgtc tggttgacgg ttcgttcggc
      301 tttggtattc attacgattg agcttgtttt aaattcctgc atttgataac ctaaagaaag
      361 aaatcccggt aaagtggagg ttaaaaaaat gctggataca aatgcacaat taccattgta
      421 cttacaatta aaagaaatat taaaaaataa aatcaataca ggggagtatc ccgagaatac
      481 gaaaattccc gctgaatcag agctgataga aagttatgag gtcagccgaa tcacagtgag
      541 aaaagcagta gaagaactgg tagaagaagg gtacctactt aaaaaacaag ggaaaggaac
      601 ttttgtcaac cggcataaag tttttcgtaa aattgaatat attatgggtt tcagtgagag
      661 ttgtaaatct aatggtttta caccgactag cattgttttg gaacgcaaga tcattccagc
      721 aactgaaggg atggcaaaaa aactcgatat cgctcaaggt gatgatgttt tatatatcca
      781 gagaaagcga atggcggata acatccccat tatgttagaa aacaattatt tttcttatga
      841 aagatatagt ttcttaatgg aggaagagct gactgattct ttgtatgaag tacttaaagt
      901 ccaaggtacc ttagccatca atcctgggga aactgcactt gaaattgtca cagctgatga
      961 ccaaattgcc aaaataatgg aagtagctgt tgggacgccc tttttcttta tggatacttt
     1021 aatcaacgat caaaacaaca taccaattca tgtaggtaat caatattacc tgggagaagt
     1081 atatcgtttt tacttatgat ttcctgtttg gatttcaagg agtagatttt atttatgaag
     1141 caaatattgc ggtcaaaaat gatttttgat ggagagcagc cacctttcaa aggttatatt
     1201 gtaatcaatg aagggatcat tgaaaaggtg gaaaacaact ggaattacag tgatctatta
     1261 aatgaaaaca cggaattaat tgcttatgat gatcaattcg ttatgccagc aattcatgat
     1321 aatcatgtat ttttcagtgg ttatttggct atgaatgctg gcatagattt aagcgataca
     1381 tcttcagcta acgaggctgt agagcgtatt aaagctgtaa tgaaccataa aaaagcagat
     1441 gaaccaattt atgctcatgg ctgggatgct gccctgtgga aaactgaccc atcagaagag
     1501 ctattgaatg aaataaacca ctttggtcca atcacagcga tcgacaaaaa tcgcagccat
     1561 tgttggatga atgctattgc taagcagact tatggtttta cagaaaaaga aaccagtgct
     1621 gaaagtcgag tattattaat taaggaaatg ctagcaaaca aaaagctttt agcagaggtt
     1681 tacgctgaat ttgaagcatt actgctttca aaaggtgtta tatcaatgaa agaaatcgtt
     1741 tttgatgacg cagaatttct ttctaatata cctcagagga aaatacttag taacctatat
     1801 gtgcaggcag tccaagaacc tctacagcat gaacttctat atgagtataa ggaaaaagtt
     1861 ttccctgaaa aggtccgctt tggtggagtg aagatcatgg tagatggtgt tgttgcggat
     1921 agaagtggag atatttacgg tgtttatcag tcaggagtag cttcacccga gattgactat
     1981 gaggcaatta aagcagaagt tgctgcattc aatgaagtag agattccttg ctgtttgaca
     2041 acggaaggag ataaagcggg ttataaagct gcgtcaattc ttgtcgaatt tggaaagcgg
     2101 ttacctgaag gcgtttttaa ttcgattagt gatttggaaa tggttactca aaagacagca
     2161 gaaaaaatga acgaaggaca agtagtagca gaggtatatc cacaaattct cggcttgaat
     2221 caaacgttag aagaggctta ca
//
LOCUS       DAYYFU010000152         2232 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000152 DAYYFU010000000
VERSION     DAYYFU010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.158"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..543
                     /locus_tag="ACHA34_002909"
     CDS             <1..543
                     /locus_tag="ACHA34_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8793470.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(899..1471)
                     /locus_tag="ACHA34_002910"
     CDS             complement(899..1471)
                     /locus_tag="ACHA34_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8793471.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1487..2092)
                     /gene="fic"
                     /locus_tag="ACHA34_002911"
     CDS             complement(1487..2092)
                     /gene="fic"
                     /locus_tag="ACHA34_002911"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFM8793472.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          701 a          453 c          338 g          740 t
ORIGIN      
        1 atttcaaaag agaatctctt aaatcaggac ttttctactg aaacgatctg tgagaaatgg
       61 gcagctgaca ttacgtacat tcctactaag aaaaatggtt ggtgttactt atcttcaatc
      121 atggatttac acacgaaaag aattattagt tatacatttt caaaacgaat gactgtggat
      181 tgtgtcattc aaacattgaa taaagcaaaa atacattatc acattccaga aggaatgatt
      241 ctacacactg acttgggcag ccaatacaca gcaagagaag tggaacaatg gcttaaaacc
      301 aacaaaataa ggcattctta tagtagaaaa ggaacacctt atgataatgc tggaatcgaa
      361 tctttccatg cctcattgaa aaaagaagaa gtctacacga ctagctactc agattttgaa
      421 gaagcaaatc gagcgctatt tagctacatt gaaggatttt ataaccgaaa tcgaatccat
      481 agctcgattc actatctaac cccacaggaa tttgaagaat tagcaaaaga aaaaatggct
      541 taaccgtctc tactaaagac aatcaacacc gtagggatat ggagataaaa tcaatagagt
      601 caatagatta tttatcgata ataacactaa aaaagccacg ttcctaaacg tgacttacgc
      661 tgaacgatat gtgttgtttg gtagacgaac aaatcgttca ttataaatat atgatggtat
      721 cctaatatag taagcgtggc tagcatacct ttaattactt taccttttac agttaccata
      781 cacatttttt gagcagaaat caagtaaata aaaaaatatt atatttcaca acatgctaat
      841 agactcatat ctagaacgca ttaagtattt acctgtctgg ttcgctgaga cttctttttt
      901 aactttcttc aattttcttc tttattctaa aaagcgtact gcgactgaaa ccggttatag
      961 cttcaacttc cttatacgat ttacctgaag taagcagttc atatgcgtgc ctcttcttcg
     1021 gtgtgattgt tgctttcgga cgtccttctc tgaaattagg attattcttt cttgcaaaca
     1081 tttttccttc ttgggtacgt tccacaatca tatcacgttc catttctgct acactcaaca
     1141 acgtccgtgt caccattcgc cccatggacg tattttcaat cgttcctaga ttcaaaacct
     1201 tgatcgttac gttatcatcg agtaaaggtt caatgatctc taatgcttct ctcgtgttac
     1261 gagcaaatcg atcaagcttc gtaacaacta tttcatctcc aggcttcaga ccagccaaca
     1321 gctgattgaa ttttggccga tcccgttttg tacccgtaaa tttttctgaa aaaatattct
     1381 tcttctctac gcagttctca agcaactgtt gaatttgttc ttttgtactt tgagaagcgg
     1441 ttgagacacg tgcatatcca tatttcgtca tttggtccac ctacttttac aaatcatcta
     1501 tcgtatactc agaataccct tcatagaaat agctgacatc gattcctttc atatataact
     1561 cgcgatcgtc aattttatca gtcagagcat tggaaattag atagcgtatt tccaaatcgt
     1621 ttatgggact tcgctccatt gcagaaagat agtctgcttt atcgattaag ttccaatcta
     1681 caactttttg caattcttcc tttaaaatga gatccagcca aattctagta cttcggccat
     1741 ttccttctcg aaatgggtga gcgatattca tctccacata tttcttgata atctcttcaa
     1801 aagaagtttg tggcatctga tcaatatgct gtaacgaatg ttctaaatac ataactggtg
     1861 caaatcgaaa atttcctttt gcaatattga ccgatcggat ttttccggca aaatcgtaaa
     1921 tatccgaaaa tagaaattca tgaatttcag ataaaccttt aaatgtacca acttcgattt
     1981 catttatttt tccacaatcg tacaattctt tcgctttttg tttactgagt ttttcttcaa
     2041 ccttagccaa ctcaacttga ttggttaatc ccaatttatt ctctaaaatc atgcgaaatc
     2101 tccttaactt tatgacataa gtttatgaaa cactctcaac cccattatat cgcgattttg
     2161 tttatgtgtc aaaaactatt aagtttctga cccccgatag atttttcaag tgtctctatt
     2221 ttgcacagtc ta
//
LOCUS       DAYYFU010000153         2203 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000153 DAYYFU010000000
VERSION     DAYYFU010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.159"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(147..617)
                     /locus_tag="ACHA34_002912"
     CDS             complement(147..617)
                     /locus_tag="ACHA34_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793473.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            704..1402
                     /locus_tag="ACHA34_002913"
     CDS             704..1402
                     /locus_tag="ACHA34_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFM8793474.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            1580..1921
                     /locus_tag="ACHA34_002914"
     CDS             1580..1921
                     /locus_tag="ACHA34_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793475.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            1914..>2203
                     /locus_tag="ACHA34_002915"
     CDS             1914..>2203
                     /locus_tag="ACHA34_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM8793476.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT          805 a          314 c          444 g          640 t
ORIGIN      
        1 aagcgttgat aggaacgatt gtttacatgg tttggatttt tatggggctt gatgaagctg
       61 aagctgagta cagagcgaaa aagccaaccg agggggctga ctaaaaatga atagaaaaga
      121 gaaactagaa tgtatattac tattactcag tttaattctt tcactaattt ctctattggg
      181 aagtttgtat ttttgatatc aacaattttg tggatttggg ttctatttag agtaaatatc
      241 atttttgttt ggtgattaag gagtttatta aaattaattt tccctacttc aaatgcaagg
      301 taacaagata tagcactttt aggaggaata tttagcggta cagcttcaga gtatattcta
      361 ttatcatcga ctgatcctcc tttagcaatt aatttcttaa atggagtagc tgaccatctg
      421 cggtttaagt tagcggatga aagttctaaa ttaactaaca tagctggttc tgttgaatag
      481 ttagaaatga ttacttttgt ataaatcata tctgcagcga agtaagaagc attaagttca
      541 acttctagtt gagggcgcat tccttttatt tttatccctg ttaaaaacgt actcaatata
      601 aaacttatta ttgacatcca ttgaaaaatt gttaagtgaa aaaaattcaa aataaacacc
      661 accagttttt aactaaatta taccaaaaag gagagaagaa ataatgcaag aattagtaat
      721 tttgaaaaat aaagaagctg tgactacgag cttacaagtg gcagaaagct tcgagaaaaa
      781 acatcaacat gttttaagag atattgatgc actaaaaaaa gatgtgtcca attttggaca
      841 gatgtttgta gaaggtaatg aaccagattc atatggcaga aatcgacgag ttttcttcat
      901 tagtagagat ggatttttct tgctggctat ggggtttaca ggaaagaaag ctatctactt
      961 caaacaaaaa tacattgaag cattcaacga aatggaagat gttattcgca agaatactgt
     1021 tcctcaaaca attgaagata tgatgatcta tcaattagaa gaaatgaaag atgttaaaaa
     1081 agatgtttcc atgcttaaag atactatgcg aattagcgga caacaagagt ttgaaattaa
     1141 gcaaaaagga aatatgaaag ttatgggagt tctaggggga aaagaaagcc gagcttatga
     1201 agaaatcagc aaaaaagtat tctcaaaatt ttggtctgaa tttaaacgta ccttttcaat
     1261 cccaagatat ggcgagttac ctcgtaagag attcgatgat gctgtttcat ttattgaaat
     1321 gtggttgcca gaaactgcga tccgtatgga aattgatcaa ctgaacagac aacagagact
     1381 ttttggtgat gacaatgaat agagctgaag cgctaagaat agggacggca attgctaatt
     1441 gctggtggaa atactataaa ccaatcatcc taagccaaca acatattgac aagcaaaaag
     1501 catggcaaca aataaaaaag tgactccgcc ggcaagcaaa gagtcacaaa gaaaacacat
     1561 cataaggaga ttttagcata tggaaaaaga actttccact ctagatcaat atttgattga
     1621 tcctgattgg ggcaagccga aaattgagga aacaagtggt cgaaaaatca gacgaaatct
     1681 tttgacgaat gaagaactag cttgggatca agatgattta ggcaaccatg taactatttg
     1741 ggatcatgtt tatcttatcc atctatcgaa gcattcgaat aaacctgaat atatttacgt
     1801 catcgaagat ggcttgattg atgcgctaga agagtacgac agagataact tgattgatat
     1861 ctcttattac ggaccaggta agaaatacat tgctgaaatg gaggcagaat ttgatgagtg
     1921 aaatcaaagg gacaacgaac tttgaaaaac tttttagtcg taagttaaat aaaattctca
     1981 agaaaaaagg aaattttgat tatttatctt gggctcacgc gtgggagatt atgaaaaaga
     2041 atgatccaca ggcaacggta actattaatg agtacaaaca ctacagggtt gtttctggaa
     2101 ctcatcaaga ctttcttgtt gaggaatata aaccttttct tatggatgaa actgggactt
     2161 atgtatctgt ctcagtaacg gttaaaggac acacggaaac cga
//
LOCUS       DAYYFU010000154         2197 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000154 DAYYFU010000000
VERSION     DAYYFU010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.160"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..418
                     /locus_tag="ACHA34_002916"
     CDS             <1..418
                     /locus_tag="ACHA34_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8793477.1"
                     /translation="SIGEFVHYLEKELTIEVNDLQKELIFNFSISKIVENLTILDPDK
                     VLSFVQKAIKELSKLLNFDFSNNQLFLLYIHCSCMIERIVRKESVDEQEDIEEYMQAN
                     GEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            431..1063
                     /locus_tag="ACHA34_002917"
     CDS             431..1063
                     /locus_tag="ACHA34_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8793478.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            1271..1495
                     /locus_tag="ACHA34_002918"
     CDS             1271..1495
                     /locus_tag="ACHA34_002918"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFM8793479.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            1597..1830
                     /gene="relB"
                     /locus_tag="ACHA34_002919"
     CDS             1597..1830
                     /gene="relB"
                     /locus_tag="ACHA34_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFM8793480.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            1820..2089
                     /locus_tag="ACHA34_002920"
     CDS             1820..2089
                     /locus_tag="ACHA34_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8793481.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT          785 a          320 c          454 g          638 t
ORIGIN      
        1 aagcattggc gaatttgttc attacttgga aaaagagctg actattgaag tgaacgattt
       61 acaaaaagaa ttaattttca atttttcaat tagtaaaatt gtcgaaaatt taaccatttt
      121 ggatccagat aaggtgctat cttttgtaca aaaagcaatt aaagaattat cgaagttatt
      181 aaattttgat ttttctaata atcagctgtt tttgttgtac attcactgta gttgtatgat
      241 tgaacgaatt gtacgtaaag aaagtgtgga tgagcaagag gacattgaag aatatatgca
      301 agcaaatgga gaaataatgg ataaaattca tagcgctttt tttgagattg aaagggaata
      361 cacaattgaa ataccagatt tagagttgcg attattgagc gatattgttc aaggatagga
      421 gtgaattcga atgacaatgg gagtaatttt tgatatggat ggggtactca tagatagcga
      481 gacattttat tttgagagaa gaatgaactt ctttcaagaa ctggagatag atccaggatc
      541 aaaccagatt gatgattatg tggggaaaac agaagctgca atatgggaaa cattagttcc
      601 tgaggacaaa tcattaagag aggaacttta tccgaaatat gtagattatc gaaaaacgca
      661 tccaattgat ttcgctaaag ctttacgaaa agaagtgaaa ccgttacttg acttcctcaa
      721 gaagcgggat ataccattgg cactggcatc ttcttctcca gctgtcgaga tagagagtat
      781 gctcattcaa tgtgaactta ctgaatattt ttcctttgtt ataagtggtg agtcattaaa
      841 acaaagcaaa ccacatcccg aaatatattt aaaagcgaaa cagcaattag actgtgactt
      901 ctttattgca gtagaggatt caacgattgg tattaaatca gcaaaggcag caaatatcta
      961 tacggcggca ctaaaacaac caatgcaaat cgatcagagt gaagcagaca tacaaattga
     1021 aagtctacaa gaactggaac aaattgtttt agaaaaaggt tagtgaatta atggaaataa
     1081 ggctatatat ctgtgctagc tattttgact aaccaaaaaa acttgctata attcaggtgt
     1141 agtaaaaggt gctggtaaca ccttttacga aaatgcttcg gcagtgataa tgggattggc
     1201 tttttaagtt ttttattttg ttcgcaatcc ctaaggtcat gtgaatgatc ctagggattg
     1261 ctagaatatt ttgcaacaga accaaaatac gaatagttat caagcagcca tattgactgc
     1321 ggtcaattta ggaggggata ctgatactac tgctgccgta actggtgctc ttgcgggtat
     1381 tctttacggg ggtgaagtga tccctgaaaa ttggagaaaa tcgcttcaaa atgaccagct
     1441 tcttaaaacg atcagtcaga gattttgcga taccgtgcta tctcaaagaa tttgaataat
     1501 ggatatgatg caaatcttgt ttgtactaac gttgtattga caaaaaggat tttatttagt
     1561 acaatgttag tacaaatgat gaacggaggt agaatcatgt ctcaatcaac cattacattt
     1621 aggattcccg acgatgaaaa agaactggta ttagaatatg caaaagcaca caattcttca
     1681 ttgaccgaac tttatcgaaa tgctgtactg gataaaatcg aagatgaaat cgatctacgg
     1741 actttacgga gtgcaattga agtttccaaa gagaaaaaag agagtggtat ttctcaagag
     1801 gaaatggaga aactgctaaa tgaattataa agtcatctac atgccgcaag cccagaagca
     1861 attaaaaaaa atggacagaa accaagctaa aataattgtc gcctggatca gaaagaattt
     1921 agaaaacacg actgacccgc gtcagcacgg aaaaggtttg actggaaata agagtgggga
     1981 atggcgctat cgaattggaa actatcgaat attggctaat atttgtgatt ctgaaattga
     2041 aatccagata ttctcaattg gacatagaag tacaatatat aaaagataac cgtttttgga
     2101 acgaagtaga gtttcgaaag tcaatgtata atcttttgaa ttttgcataa taccacaggt
     2161 agaaactacc cgatactatc ggttggtttc tgctttt
//
LOCUS       DAYYFU010000155         2065 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000155 DAYYFU010000000
VERSION     DAYYFU010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.161"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1008
                     /locus_tag="ACHA34_002921"
     CDS             <1..1008
                     /locus_tag="ACHA34_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793482.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="ACHA34_002922"
     CDS             1025..1174
                     /locus_tag="ACHA34_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793483.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="ACHA34_002923"
     CDS             1171..1617
                     /locus_tag="ACHA34_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793484.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="ACHA34_002924"
     CDS             1619..1756
                     /locus_tag="ACHA34_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8793485.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..>2065
                     /locus_tag="ACHA34_002925"
     CDS             1794..>2065
                     /locus_tag="ACHA34_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793486.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          753 a          358 c          425 g          529 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa acttt
//
LOCUS       DAYYFU010000156         2056 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000156 DAYYFU010000000
VERSION     DAYYFU010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.162"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..337)
                     /locus_tag="ACHA34_002926"
     CDS             complement(<1..337)
                     /locus_tag="ACHA34_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM8793487.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
     gene            complement(404..559)
                     /locus_tag="ACHA34_002927"
     CDS             complement(404..559)
                     /locus_tag="ACHA34_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFM8793488.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1176..1397
                     /locus_tag="ACHA34_002928"
     CDS             1176..1397
                     /locus_tag="ACHA34_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793489.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1398..1655
                     /locus_tag="ACHA34_002929"
     CDS             1398..1655
                     /locus_tag="ACHA34_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793490.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1791..>2056)
                     /locus_tag="ACHA34_002930"
     CDS             complement(1791..>2056)
                     /locus_tag="ACHA34_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005727 - extrachromosomal circular DNA
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rep family protein"
                     /protein_id="HFM8793491.1"
                     /translation="DIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLI
                     DYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          678 a          462 c          314 g          602 t
ORIGIN      
        1 ttttagcata ttgaaatttt tctggattat ccatatgagc aaaatatcgc accatacctt
       61 ttgcattagc acatttttgc gggtttggag accgaagctt ttgagttatc tcacgtattt
      121 gatcatacgt ctttttaccg ctaaacatta tcaaaacatg ccaatgaggc tttttcaatt
      181 caccgtcagg attcacatct ttatcatgaa gtggactctc aacccaagga acatgtaact
      241 catctaaaaa ctccctccaa ttttcaggag ccgattcagg atagactaca aacgtccaat
      301 tccttgtgcg ttcatcttta gtttttctac tcgtcatgct ataattacct taattgaaca
      361 acagtctaac agcaaaagca gtgcgccaac actaatgctt ttgctatttt ttttgctcat
      421 tttcctttcc gttagcatta acccaaattg taataagacc agacttacta agacccattt
      481 gtttcgcaat tttatcaaga ttatctgcca cttccttagt aagcgttaca acgactctct
      541 ttttttcatt actgatcata attcgtatcc tccaacgact ttttcttaaa aaccatagta
      601 acaaaaaagt aactaaggtg caacagttta gatactaatt agcgaataat aggccttaaa
      661 cccttggcat tactgatttt gtcaaaaaaa aacgctgaaa cccttgggag agtaaggcta
      721 aacagctatc gaccaatgac caatgaccaa taaggggggt gtcgtagtcc ccccctcctc
      781 cgcccaattc ataaacacca aaaccaaaaa acaaacctca gggctccgcc ccgaaccccg
      841 acctccttct accgaaatat atgcaggcgc cacgcttact atttcgttca gctatcggct
      901 tttgcaatct aacacgaccg tttcgctacc agtcgcctta caggctccat tctcgcttct
      961 cttacgtgtt ttaacgattg cgccgataac taaacgaaat agacgctaaa tcgcttaaaa
     1021 cgcaaaatag acccttttta gagcattgat tttttctact agtcatttcg ataatataaa
     1081 attgaccaca acacagaaaa cttatattat aagcttatgt cgtaagtccg acgtactcac
     1141 gtaatataga cggacgttaa atggaaggag cataaatgag agtgataaaa tcagaaaaat
     1201 tcactttggc cgaaattatg acgaccagcg aacttgcaca agaacttgat tacaaccaag
     1261 cttacgtttt gcgcttagcg aaagaacatc tgactgaagg aaaagaatat cgctctgccg
     1321 gtagaagaaa ctatcttttc agcccagaag cccttaaaaa actagaaaaa gtcataacca
     1381 ccaaagaagg tgagtaaatg cctaaagaaa caactatcaa cgttgaacaa ctagaaaaat
     1441 tgctagaatc atattccaat gattttatcg aattgaccgg cggaaaagtc gaagaaacca
     1501 ccgacctctt tgacctactt ctcacaaata ttctggaatt aaactctgaa gaacaaaaag
     1561 agcataaggg aaaactgata gcaccttcct acaggaaggt ggctaagtcg gctgtgccaa
     1621 ctggttttct tacaaaacaa ccttatattt tttgatatca caaaaaaatc cattttggac
     1681 ctattttcgg tcataatata gtacctactt ttggtcatag tttcgtccgt agttttggtc
     1741 atagtgacta agactttcac gcacaaaaaa agagctgaaa ttaacagctc ttatccttta
     1801 tttatttttg aatttacact tccaccatga cgattcgatt ttatatattg cccgataata
     1861 taagctgaat tatcacaaag aagcggaaac caatcatcaa atctttctga cattgcatag
     1921 tcaattaaat ctttgatttc agtgatattc ttagaatcaa caaacgacat catctctcta
     1981 atcaactcat atcgttctgc actcgttact gacaaataac tagcaatttc tgcaccacca
     2041 tgagcgataa tatcac
//
LOCUS       DAYYFU010000157         1991 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000157 DAYYFU010000000
VERSION     DAYYFU010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.163"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            272..838
                     /gene="amaP"
                     /locus_tag="ACHA34_002931"
     CDS             272..838
                     /gene="amaP"
                     /locus_tag="ACHA34_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8793492.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            851..1039
                     /locus_tag="ACHA34_002932"
     CDS             851..1039
                     /locus_tag="ACHA34_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8793493.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1052..1609
                     /locus_tag="ACHA34_002933"
     CDS             1052..1609
                     /locus_tag="ACHA34_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8793494.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1641..1883
                     /locus_tag="ACHA34_002934"
     CDS             1641..1883
                     /locus_tag="ACHA34_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8793495.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          723 a          308 c          406 g          554 t
ORIGIN      
        1 ataaatagaa tcttgtaata ataactgagc ataatctttg caatttttaa tcttgaataa
       61 tatcctttaa gtatagccag caatcaaaag taaatagaga ataagtaagc aattgttatt
      121 tcagtatcag actggcaata aaacgccgtt atgatccagc aatgtttggt agcgctttat
      181 ttactgatgc tatgctaact gttcgctatc tatactgtga ttgtcggtag agttatttac
      241 agggtaaaaa taagtttata ggaggtggaa aatgaataag gggttaaaaa taatcggtgt
      301 tatcatgagt ttactgctac tttcagtatt tctagcgatt gcgttgatca atgcgccgta
      361 tattatgcct gaacaattgg aacgatttcg cttctttact ttaacgaatt actacatgca
      421 acaatatatt ttctgggcag ctgtagtatt tgccgttctt gctattatca tcttgctagt
      481 cgttcttttc tatccaaagt caagaggaac tttcgttatg aaacgggaag atggcaaact
      541 aacaatcgac aaaaaagcga tcgaagggtt ggtacgttcc catttacacg aagaagagtt
      601 cattcattca cctaaagtac ggatacgttc aacgaaaaat cgaatccata tccatgtcaa
      661 cggagacttg aaacgaactt cttcattagt agggaaaaca ggtgtattga tgcaagatat
      721 cgaagaagaa gtaactaaag tactcggaac aaaagaaacg gtcaaagtgg cagtcacata
      781 cagcggttat gaagaacaag aagatacaag agactacaag cactctcgag tggaatagga
      841 gggccaaata atgaaagaac tattttcagc atacaagctc cctattatct ttgggggtct
      901 tggactactg ctagctgttt tatggttaac gattggattt ttcaagacat tgttgatcat
      961 aatcatgaca gcaattggcg caggaatcgg tttttatttg aaacagtcag gaatagttga
     1021 tcaatttttt aaatcatagg aggaaacaaa gatggaaaac aaaaacatga atacaacaaa
     1081 tggatcagtt ttggcaaatg aaaaaggaga aaaccgcatg atgaatggtg aattgactta
     1141 tgaagacaaa gtcatccaaa aaatcatcgg ttatgcattg aaagatgtag atggtctatt
     1201 gacattagat ggcggatttt tctcaaatat ggctgaaaaa ttagtcaaca cagataacgt
     1261 aacagcaggt atcaatacag aagtaggtaa gaaacaagta gctgtggatc tagatgtgat
     1321 cgtagaatac ggtaaagaca tcgaagatat ttacaaccaa atcaaagaat tgatcagtac
     1381 tgaagtaaac aatatgacac atttagatgt tatcgaagtg aatgtgaatg ttgcagacat
     1441 caaaacacaa gaagaatacc aaaaagatca agaaacagta caagacaaag taacagaagc
     1501 agcaaaatcc acaggacaat ttgcttcacg ccaaacagat aaagcaaaat ctgctgtagg
     1561 caaaggtgca caaaaagtca aagaaaataa cgaaccacgt gtacaataag aagtaagaag
     1621 tcagttagga ggaatttaaa atgggattta tttgggcatt aatcgtcggc ggtatcattg
     1681 gagcaattgc aggagcaatc acgaataaag gatcatcaat ggggatcatc gcaaacatca
     1741 ttgctggttt agtaggttca gcaattggac aagctttact aggaacttgg ggaccaacaa
     1801 ttgcaggaat ggctatcatt ccatcattga tcggtgcaat cattttagta ttagtcgttt
     1861 cattctttgc acgtaaatta taagcaaatt gaagaaaaac ttatgttgaa atgaagaaca
     1921 aatttgagaa ccgtagcgac actcgctgcg gttctttttt atatatttta tatgaagtat
     1981 tctttattcc t
//
LOCUS       DAYYFU010000158         1962 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000158 DAYYFU010000000
VERSION     DAYYFU010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.164"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..272)
                     /locus_tag="ACHA34_002935"
     CDS             complement(<1..272)
                     /locus_tag="ACHA34_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793496.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
     gene            complement(309..446)
                     /locus_tag="ACHA34_002936"
     CDS             complement(309..446)
                     /locus_tag="ACHA34_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8793497.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(448..894)
                     /locus_tag="ACHA34_002937"
     CDS             complement(448..894)
                     /locus_tag="ACHA34_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793498.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(891..1040)
                     /locus_tag="ACHA34_002938"
     CDS             complement(891..1040)
                     /locus_tag="ACHA34_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793499.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            complement(1054..>1962)
                     /locus_tag="ACHA34_002939"
     CDS             complement(1054..>1962)
                     /locus_tag="ACHA34_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793500.1"
                     /translation="LLDDPYWVGKTSLGENIANKGTTFPIKSSAYEIYKGNTEEELII
                     GQTYTITLKGTKPASQTFVAYNYWNVNFGELKPVEGLTDVWSLTFTPTKLEPGLPKDL
                     RIFQLPKETAGACQIDWLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDITPEYT
                     EKGLNDTVSLTEPQSIEGLKNFEDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKL
                     YREGKTVTIYFFALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSAYSFVITYSI"
BASE COUNT          509 a          407 c          342 g          704 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcatagtg gaacgaatag tcgaacttga tcgtcagtaa aacagcccca atcatacatc
      421 attttcacat cgctaaaact aaacatacta ctcacctcct tttgaagctg gatttagttg
      481 ctctttaatt tctgaaatgt ctttgctatt ttgtaacgaa gcaagcatca tttttgaatt
      541 gatttgtgct aaactgtcag ctttttcttt caatgcagta ttttcctgtt taatcgctac
      601 atcgcttagc atgagtttgg catttagctg ttttaggttg ccgttttcat gttcaagagc
      661 ctcgtacatc gctttgagat tgtttaaatc gttgtgatct aacgcgttcg ctaaaataat
      721 ccattggttc aatttaggat caaacatctg atcagcaatc gttagcggtt cgccatctga
      781 acgaacccct tcgagcggtg gctgatctgt gtaaggaacg gatacaagca tgtcgtccaa
      841 tacttttcct gcatactctc cgccagtacg gccatatttc caaatgtttt tcatttattt
      901 ccctcccagt aattgaattt cggtttccaa tttggaatcg gtggttcgac ttctgtgcat
      961 tcttccggta aatgttcttc gtcattcaca atgatttgct cgaatccgta aggcttgatt
     1021 gatttgtaaa ctactttcat tagatttcct cccttaaatt gaataagtga taacaaatga
     1081 ataagctgat ccataacttg agtttcttcg ccatttaatg gctccatctg caccaataga
     1141 taactgagca ctgttcaaag tagaacggtc tatcgagcca accagttgct caaaactaat
     1201 tggtggccga tagccttctg gaattgttag tatcgttgaa tcatttccac cactgctttt
     1261 cccattcaac gcaaaaaaat aaatagtgac ggtttttcct tcacgataaa gctttgctga
     1321 tccggtattc ccgtttgtaa ctgttaaggt tacgacttca tatttgttgt cgtctgatgt
     1381 caatacagat tttccttttg attgaatacc atcttcgaaa tttttcaatc cttcgatgga
     1441 ttgcggttca gtcaaactaa ccgtatcatt caagcctttt tcagtatatt caggcgtgat
     1501 gtcccagctg tagtcgttcg gattgttgct atctttcaat ccttcaccaa agtatttaaa
     1561 ctgactaata ttcggggttc gggtgtcgcc tttttcaatt ttgagccagt caatttggca
     1621 tgcgccggcc gtttctttag gcaactgaaa aatgcgaagg tctttaggca aacccggttc
     1681 aagtttcgtt ggtgtgaatg ttagagacca tacgtctgtc aatccctcaa ctggttttag
     1741 ttctccaaaa ttaacgttcc aataattata tgctacaaag gtttgacttg cgggttttgt
     1801 tcctttaagc gtgatagtat acgtttgacc tattataagc tcttcttccg tgttaccttt
     1861 atatatttcg tacgcgctag atttaattgg gaacgtcgta cctttatttg caatattctc
     1921 acccaacgat gttttaccta cccagtaagg gtcatctagt aa
//
LOCUS       DAYYFU010000159         1921 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000159 DAYYFU010000000
VERSION     DAYYFU010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.165"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..358)
                     /locus_tag="ACHA34_002940"
     CDS             complement(<1..358)
                     /locus_tag="ACHA34_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8793501.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDG"
     gene            complement(355..1008)
                     /locus_tag="ACHA34_002941"
     CDS             complement(355..1008)
                     /locus_tag="ACHA34_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8793502.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1296..>1921
                     /locus_tag="ACHA34_002942"
     CDS             1296..>1921
                     /locus_tag="ACHA34_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM8793503.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT          607 a          345 c          246 g          723 t
ORIGIN      
        1 taccatcatt aagctttatt tctctttctt caattatcaa agaattacta tcactttcta
       61 aattaacatt tatattatct ttgatttgta tttcattctt attatttttt tcttttatga
      121 atacttttat ttcactactc ttggaataaa actccaaagt ttgttctata tattttattt
      181 cttttccatt catattacta gaaatttcat ttgccatatt atgaatcttt tcttttctcg
      241 tctccaaata tgttttagga aagataaaaa aaattaatga atgaactaat ataattatta
      301 ttccaagaac agaaaatatt tgtatgaaca tttttggaaa tatctttaat tttttcattt
      361 tctctccaat ttatatccta catttcttat agtagttata cagtctaatt gtaatttttt
      421 tctaagttct tttatatata catctattac tcgatcataa ggagtttctt cgctatcttt
      481 ccatacataa tcaataattt gcattcttgt taacacttgt ccattattat ccaataaaca
      541 ttttaatact tctagttctt ttgcatttac atctatttct tcaccattta ttttagctgt
      601 atagctatta aaattaaccg atagatcctt atattcaaac ttctctaaat gtccataatt
      661 cttcttaatt aaagaatcta ttctagcttt taagaccggc aatgaaaatg gcttttctac
      721 atatccgtct actaaattag taaatgcatc aattttatat tcttcatcac taaatgcagt
      781 caagattaga attggtaaat tgcttttctt tctaatttct ttcaacactt ctaaaccatt
      841 tataaaaggt atctgaatgt ctaagataac caaatttata tcgctattaa attttgacaa
      901 ggcttctctt ccatctttag cttgaataac agtatatcca aattctgaaa gatattcact
      961 tattccctct cttatcatat tatcatcttc aacagttaat attttcatat atacctccac
     1021 ttaagcctat acactagata ttatacagca tattatggct ctataatatc tagtgttggt
     1081 gacaccttct gttgccaata ctttttcttt tttctaataa atgcaaacag acaccttgaa
     1141 atcctttaga ataaagggtt ctataatatt ttgtgttgtt ggaaaatcga aggcgatttt
     1201 ctactaacac tttttcttcg tttcaactca aaaagagact cacttttggt aaaatttagt
     1261 cgaccaaaac atcaccctac caaaggagtc gctctatgga taatcatact agaaaattac
     1321 tcggattaac agataaacat ttattttttg atgaagaatg gctgattgaa aaagaatata
     1381 aaggcgttca agcgcagttt attaaaggga gattggatga ctcaccttcc cattgtgaac
     1441 actgtggatc gattcgtatt atccgcaacg gttcctatac gacacgtact cagatattga
     1501 aagtcaaaga aaagttgact attttagaat taaaacgtac ccgttttcta tgctatgatt
     1561 gtgggcagac cttttctgcg aaaacggact tagttgatga acatcatcaa ctaacaaagg
     1621 aactgaagca ggcgattctc atagaactgt acgaaaatca atcacgtaaa ctaatcgcta
     1681 agaagtactt cgtctcggat gggacggtga cacggatctt gcgtgaagca acgaagcatt
     1741 accagccacg tatgaacttc cttccgacgg tgttgtgtat ggatgagttc aaatcaatga
     1801 agtctgtttc tggttccatg agttttattt gtgttgatgg gacaacccat cagctattta
     1861 cgatccttga agatcgcaga ctttacaaac taactcaata cttcctgcgc tttcctagaa
     1921 a
//
LOCUS       DAYYFU010000160         1859 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000160 DAYYFU010000000
VERSION     DAYYFU010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.166"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..221
                     /locus_tag="ACHA34_002943"
     CDS             <1..221
                     /locus_tag="ACHA34_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8793504.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            218..1237
                     /locus_tag="ACHA34_002944"
     CDS             218..1237
                     /locus_tag="ACHA34_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8793505.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            1426..1499
                     /locus_tag="ACHA34_002945"
     tRNA            1426..1499
                     /locus_tag="ACHA34_002945"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1460..1462,aa:Ile,seq:cat)
BASE COUNT          610 a          327 c          374 g          548 t
ORIGIN      
        1 ataataaaac gttcgaagta ctaaaatggt tcgcactggt aattattccc gcactagcta
       61 ctttcgtggg gttagttggt aaagcgctca attggcagta cacagatatc tgtgttgtca
      121 tcattactgg ttttggcgcg tttttaggga gtgtgttggg tgtatcaaat cgaacctaca
      181 aaatgttctc ggctgaaagc gaagaaggag gaaacaaatg aaaaagaaaa ttactattac
      241 tgcgatgagc ctgttaacgg ctcttttttt attgccaatt aacggatttg cctatactat
      301 caacaatgaa tttaatttgg gcgcaaatga aggtagctca caagtagcaa ataatcagta
      361 tattttactg catgaaacgg ctaatgaaac agcaacagga cgcaatgaag cgcagtatat
      421 gcaacgttca tggactagcg cttatactgc ttatattgtg ggagacggcg gaattgttta
      481 ccaagtcgga caacctggtt atgtacagta cggtgctggt tcatatgcta atgccaatag
      541 tcctgtgcag attgagttac aacacacaca tgataaagca acttttgaaa aaaactataa
      601 agcatacgtt gaattggcta gagattcagc aatgaaatat ggtattccat taacattaga
      661 cacgccttat aaccaaccag gaatcaaatc acatttatgg gtaactcaaa acatttgggg
      721 cgatcacact gatccgtatg gatacttatc agaaatgggt gtaagtaagg aaaaactggc
      781 ttacgattta gctcatggat ttacagacga aaacccaacg acttctgaga acaagcctgt
      841 cattgatcca acccgagcag gtgcagcaaa tccaacttta tcagatggaa cgaaccattc
      901 tcacattgat cagtttggag aaatcgaaaa tgcgaactta cacgtcgctg gatggcacat
      961 cgctaactat aagtacgagt atattttcat tatggactac aatactggga aagaattagc
     1021 tcgagtaaga gctgatggaa tttatagacc ggatgtaaat caagcttata atacttctgg
     1081 aaatgttggc tatcatgtat cttttaacat gcgtaacttc cctaataaga aagtctatgt
     1141 catgatgcgg gcaacgaatg atccagaggg aaacactaaa ggcggtgcgc aagatttcca
     1201 tgacaaacgt tggtatttaa atattcctaa acgataaaaa tagctcctcg ttgaggagca
     1261 ttacataact atattgacaa ctataaaaat cattcgataa aatagtgatg ttatcgcata
     1321 tcttcactat cacccataat agtcacactc caagctatgc gataacaggt ttgttgccac
     1381 acattctact ggttgattgt ttatggcttt atgtggcaac aaccagtacc cttagctcag
     1441 ttggtcagag cagacggctc ataaccgtcc ggtcgtaggt tcgagtccta cagggtacat
     1501 taacgtagcc atttgaatcg ttctgtgtta gaattttttg aagagtatta tacaagctaa
     1561 agcttttctt cattgccact caaatgagtg gcttttttat gtatcctttt atggattaat
     1621 gaaaggatgt ttcacatagt tatatttctg tatatttgaa aagttttact ttgattttta
     1681 aatagaaaga catttgggtt atattgtgag ataataataa agaagagttt aaagcgttcc
     1741 ccaaaaacca ctgccccata agtgtgttac gctttaaact cttttatatt tgaagccatt
     1801 aaaaagcata ccatattttt gaaaaaaagt gagaaaaaag gcttataatt ggagtggta
//
LOCUS       DAYYFU010000161         1845 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000161 DAYYFU010000000
VERSION     DAYYFU010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.167"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..375
                     /locus_tag="ACHA34_002946"
     CDS             <1..375
                     /locus_tag="ACHA34_002946"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8793506.1"
                     /translation="DDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYTLLRPD
                     GKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEK
                     LGGKNDYKDEIVYVYDYEKGDK"
     gene            376..1815
                     /locus_tag="ACHA34_002947"
     CDS             376..1815
                     /locus_tag="ACHA34_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HFM8793507.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          772 a          137 c          313 g          623 t
ORIGIN      
        1 gatgataata tggtttttga tattgataat ttaaaaggat ttcttaatga taccagttca
       61 tttgggttta tagctaaaga aaataataaa attataggat ttgcatattg ctatacactt
      121 ttaagacctg atggaaaaac aatgttttat ttacactcaa taggaatgtt acctaactat
      181 caagacaaag gttatggttc aaaattatta tcttttatta aggaatattc taaagagatt
      241 ggttgttctg aaatgttttt aataactgat aaaggtaatc ctagagcttg ccatgtatat
      301 gaaaaattag gtggtaaaaa tgattataaa gatgaaatag tatatgtata tgattatgaa
      361 aaaggtgata aataaatgaa tatagttgaa aatgaaatat gtataagaac tttaatagat
      421 gatgattttc ctttgatgtt aaaatggtta actgatgaaa gagtattaga attttatgat
      481 ggtagagata aaaaatatac attagaatca ttaaaaaaac attatacaga gccttgggaa
      541 gatgaagttt ttagagtaat tattgaatat aacaatgttc ctattggata tggacaaata
      601 tataaaatgt atgatgagtt atatactgat tatcattatc caaaaactga tgagatagtc
      661 tatggtatgg atcaatttat aggagagcca aattattgga gtaaaggaat tggtacaaga
      721 tatattaaat tgatttttga atttttgaaa aaagaaagaa atgctaatgc agttatttta
      781 gaccctcata aaaataatcc aagagcaata agggcatacc aaaaatctgg ttttagaatt
      841 attgaagatt tgccagaaca tgaattacac gagggcaaaa aagaagattg ttatttaatg
      901 gaatatagat atgatgataa tgccacaaat gttaaggcaa tgaaatattt aattgagcat
      961 tactttgata atttcaaagt agatagtatt gaaataatcg gtagtggtta tgatagtgtg
     1021 gcatatttag ttaataatga atacattttt aaaacaaaat ttagtactaa taagaaaaaa
     1081 ggttatgcaa aagaaaaagc aatatataat tttttaaata caaatttaga aactaatgta
     1141 aaaattccta atattgaata ttcgtatatt agtgatgaat tatctatact aggttataaa
     1201 gaaattaaag gaactttttt aacaccagaa atttattcta ctatgtcaga agaagaacaa
     1261 aatttgttaa aacgagatat tgccagtttt ttaagacaaa tgcacggttt agattataca
     1321 gatattagtg aatgtactat tgataataaa caaaatgtat tagaagagta tatattgttg
     1381 cgtgaaacta tttataatga tttaactgat atagaaaaag attatataga aagttttatg
     1441 gaaagactaa atgcaacaac agtttttgag ggtaaaaagt gtttatgcca taatgatttt
     1501 agttgtaatc atctattgtt agatggcaat aatagattaa ctggaataat tgattttgga
     1561 gattctggaa ttatagatga atattgtgat tttatatact tacttgaaga tagtgaagaa
     1621 gaaataggaa caaattttgg agaagatata ttaagaatgt atggaaatat agatattgag
     1681 aaagcaaaag aatatcaaga tatagttgaa gaatattatc ctattgaaac tattgtttat
     1741 ggaattaaaa atattaaaca ggaatttatc gaaaatggta gaaaagaaat ttataaaagg
     1801 acttataaag attgattata taatatatga aaagctatta taaaa
//
LOCUS       DAYYFU010000162         1833 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000162 DAYYFU010000000
VERSION     DAYYFU010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.168"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACHA34_002948"
     CDS             <1..969
                     /locus_tag="ACHA34_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM8793508.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1159..1232
                     /locus_tag="ACHA34_002949"
     tRNA            1159..1232
                     /locus_tag="ACHA34_002949"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ile,seq:cat)
BASE COUNT          605 a          317 c          346 g          565 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttctgtg
     1261 ttagaatttt tttgaagagt attatacaag ctaaagcttt tcttcattgc cactcaaatg
     1321 agtggctttt ttatgtatcc ttttatggat taatgaaagg atgtttcaca tagttatact
     1381 tctgtatatt tgaaaagttt tactttgatt tttaaataga aagacatttg ggttaaattg
     1441 tgagataata ataaagaaga gtttaaagcg caccccaaac cacttcccca taagtgtgtt
     1501 acgctttaaa ctcttttata tttgaagcca ttaaaaagca taccatattt ttgaaaaaaa
     1561 gtgagaaaaa aggcttacaa ttggagtggt agttaattag tgacttattt ttgattttat
     1621 agcactgata ctataaaata tagatatcat catattacac aatcttaata ctaacttaaa
     1681 aaatatctcc tttcacaagt atggtgataa aattcgttcc gggctacctt tttaggtagc
     1741 ctactttaat ctttatacct ttctggatca acgaaagtat actttatata gtcataacgc
     1801 cgatgatcgc tacgtgcgtc cggcacgtca gtc
//
LOCUS       DAYYFU010000163         1796 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000163 DAYYFU010000000
VERSION     DAYYFU010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.169"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="ACHA34_002950"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="ACHA34_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8793509.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          503 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DAYYFU010000164         1747 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000164 DAYYFU010000000
VERSION     DAYYFU010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.171"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1412
                     /locus_tag="ACHA34_002951"
     CDS             <1..1412
                     /locus_tag="ACHA34_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8793510.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..>1747
                     /locus_tag="ACHA34_002952"
     CDS             1546..>1747
                     /locus_tag="ACHA34_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8793511.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTL"
BASE COUNT          693 a          258 c          372 g          424 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaa
//
LOCUS       DAYYFU010000165         1573 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000165 DAYYFU010000000
VERSION     DAYYFU010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.172"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            828..980
                     /locus_tag="ACHA34_002953"
     CDS             828..980
                     /locus_tag="ACHA34_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793512.1"
                     /translation="MYDICEEAGGIVECPKNATRNEIELSDFIVTHSGGDESVIEFIE
                     WILESI"
BASE COUNT          594 a          179 c          320 g          480 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caaaggagtg taa
//
LOCUS       DAYYFU010000166         1551 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000166 DAYYFU010000000
VERSION     DAYYFU010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.173"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..344
                     /locus_tag="ACHA34_002954"
     CDS             132..344
                     /locus_tag="ACHA34_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793513.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="ACHA34_002955"
     CDS             506..1024
                     /locus_tag="ACHA34_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFM8793514.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="ACHA34_002956"
     CDS             1031..1543
                     /locus_tag="ACHA34_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793515.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
BASE COUNT          611 a          181 c          241 g          518 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt a
//
LOCUS       DAYYFU010000167         1533 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000167 DAYYFU010000000
VERSION     DAYYFU010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.174"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..1486
                     /locus_tag="ACHA34_002957"
     CDS             20..1486
                     /locus_tag="ACHA34_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFM8793516.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          421 a          289 c          326 g          497 t
ORIGIN      
        1 aagaattggg gtatagatta tggaaaaaca gcaagacaag ggcttcttcg ggcagccgaa
       61 gggattgtca actttgttct tcactgagat gtgggaacga tttagttatt acggtatgcg
      121 tgcattactt atctattata tgtacgatac agttgccaac gggggacttg gattaccacg
      181 agcaacagca ttagctatca tgtcgatcta cggatctttg atctacatgt ctagtatcgt
      241 cggcggatgg gtatctgacc gagtgttagg agcaaaacgc actgtattct tcggtggagt
      301 tttcattatg cttggccaca tcgtcttggc tttaccattt ggtgtaagta cattgtttgt
      361 ttcgatggca ttgattatta tcggaaccgc ctttttgaaa ccaaatgttt ctggcatggt
      421 cggacatctt tattcaaaga ctgacttgcg aagagatgca ggattttcga ttttttacat
      481 gggtgtcaac ttaggggcat tattagcacc actaatcgtt ggtacaatcg gtcaagaata
      541 taactatcat ttagggttct ctttagctgc tattgggatg ttctttggat tattacaata
      601 catcatccaa ggacgcaaga cattagacgg agtgggtaca acaccgccga atcctttgac
      661 aactgaagaa cgtaaaggat tcatccgtaa cttgatcatc gcacttgttg ttatcgctgc
      721 agtattcggc ggggcacaac taactggtca tctgacaatt gacttttttg tcaatacaat
      781 cagtgtattg gggattatct taccgttgta ttactttatc aaaatgctga cttcaaaaga
      841 tgtaactgct gaagaaaaac caagagtatg ggcttatatc ccattattcc ttgcagcaat
      901 cattttctgg tcattacaag aacaaggttc atctatctta gctttatttg ctagtgaacg
      961 tacacaatct acgctatttg gtgtaacgat ccaagcaagc tggtatcaat cattgaatcc
     1021 aattttcatc tttattctga caccagtatt cgtaacgatg tggacgaaac ttggtgaacg
     1081 tcaaccatct acagtagtga aattctctct tggactttta tttgcaggat tatcgtactt
     1141 gctattgatg ttgccgggta tgctttatgg tacaagtgga aaagtaagtc cattgtggtt
     1201 gttgggaagt ttcttcatcg tggttattgc tgaattgtgt ctttcaccag ttggtttgtc
     1261 tgtgacaaca aaattagcac cacgagcatt tgaatcacaa acaatcgcta tttggttctt
     1321 agctgatgct tcttcacaag caatcaatgc acaaattgca cgcttctaca caccgggaac
     1381 agaatctgct tacttcggta ttatcggtgc agtagcaatc gtcggtggta tcttgctatt
     1441 tactgtgaaa gaaccaatca aaaaactaat gggctctatt cattaatcaa tgaacataaa
     1501 aacaggaatt gcttatttgc agcagttcct gtt
//
LOCUS       DAYYFU010000168         1509 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000168 DAYYFU010000000
VERSION     DAYYFU010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.175"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..868
                     /locus_tag="ACHA34_002958"
     CDS             101..868
                     /locus_tag="ACHA34_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM8793517.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1060..1458
                     /locus_tag="ACHA34_002959"
     CDS             1060..1458
                     /locus_tag="ACHA34_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFM8793518.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          561 a          189 c          282 g          477 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct
      901 tcagacgtat gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt
      961 taattagctt gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa
     1021 aaagcaaaaa ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca
     1081 gtattcgaat ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat
     1141 acacatggtg aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac
     1201 ttggtgcttt tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta
     1261 aatgcaagaa tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa
     1321 gttgctaaag ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat
     1381 ttaaatgttt tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg
     1441 agagaaaagt tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta
     1501 ggacgtatg
//
LOCUS       DAYYFU010000169         1477 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000169 DAYYFU010000000
VERSION     DAYYFU010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.177"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            288..695
                     /locus_tag="ACHA34_002960"
     CDS             288..695
                     /locus_tag="ACHA34_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793519.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="ACHA34_002961"
     CDS             709..1110
                     /locus_tag="ACHA34_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793520.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..>1477
                     /locus_tag="ACHA34_002962"
     CDS             1112..>1477
                     /locus_tag="ACHA34_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793521.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKL"
BASE COUNT          566 a          181 c          258 g          472 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactc
//
LOCUS       DAYYFU010000170         1451 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000170 DAYYFU010000000
VERSION     DAYYFU010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.178"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..625)
                     /locus_tag="ACHA34_002963"
     CDS             complement(<1..625)
                     /locus_tag="ACHA34_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFM8793522.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="ACHA34_002964"
     CDS             complement(784..1050)
                     /locus_tag="ACHA34_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8793523.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="ACHA34_002965"
     CDS             complement(1040..1396)
                     /locus_tag="ACHA34_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8793524.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          405 a          279 c          181 g          586 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg a
//
LOCUS       DAYYFU010000171         1440 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000171 DAYYFU010000000
VERSION     DAYYFU010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.179"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..1406
                     /locus_tag="ACHA34_002966"
     CDS             105..1406
                     /locus_tag="ACHA34_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8793525.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          474 a          288 c          264 g          414 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
//
LOCUS       DAYYFU010000172         1417 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000172 DAYYFU010000000
VERSION     DAYYFU010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.180"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACHA34_002967"
     CDS             complement(150..1337)
                     /locus_tag="ACHA34_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFM8793526.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAYYFU010000173         1355 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000173 DAYYFU010000000
VERSION     DAYYFU010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.182"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1355
                     /locus_tag="ACHA34_002968"
     rRNA            <1..1355
                     /locus_tag="ACHA34_002968"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAYYFU010000174         1324 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000174 DAYYFU010000000
VERSION     DAYYFU010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.184"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..820
                     /locus_tag="ACHA34_002969"
     CDS             278..820
                     /locus_tag="ACHA34_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793527.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..>1324
                     /locus_tag="ACHA34_002970"
     CDS             976..>1324
                     /locus_tag="ACHA34_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFM8793528.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          533 a          157 c          248 g          386 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccag
//
LOCUS       DAYYFU010000175         1294 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000175 DAYYFU010000000
VERSION     DAYYFU010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.185"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1244
                     /locus_tag="ACHA34_002971"
     CDS             <1..1244
                     /locus_tag="ACHA34_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFM8793529.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAYYFU010000176         1272 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000176 DAYYFU010000000
VERSION     DAYYFU010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.186"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1272
                     /locus_tag="ACHA34_002972"
     CDS             <1..>1272
                     /locus_tag="ACHA34_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFM8793530.1"
                     /translation="SIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRACRS
                     CGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYF
                     VQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSKKIL
                     PRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEEVEF
                     LVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKSQAE
                     KLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAYPFF
                     HELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNGKIE
                     AKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          426 a          249 c          244 g          353 t
ORIGIN      
        1 tctatcaaaa aaatgctgag aataacagag aaagatttga tgattacaga ggtctcttac
       61 gagacccttc agaagaaaaa gacgttggtc gtcgatgctg ttctctcgcc tactcctcgt
      121 gcttgtagaa gttgtggttc tactgtggta gatggaaacg ggaaagcaat tatagtgaaa
      181 aatggaaaaa aggaaacgat tgtccgtttt gaacaataca atcatatgcc tttggttatg
      241 cgcctaaaaa agcaacgcta tacctgtaaa aactgtcgca cccattggac ggctcaaagt
      301 tattttgtcc aacccagaca ttcaatcgca aatcatgtta gatataaaat tgcttcttta
      361 ctgactgaaa aagtatcttt atcttttatt gcgaaaagct gtcaggtatc tttgaccacc
      421 gttattcgta cattgaaaga gtttaaaagc tatttaccaa agcaatctaa gaagatcctc
      481 cccagagtat tgatggttga cgaatttcgt tcgcatgctt ccatagaaga taagatgagc
      541 tttatttgcg cggatggcga aacaggaaaa ttaatagatg ttttgcctac gcgtaaatta
      601 cctcgattaa caagctattt cttaggttgt accaatccag aagaagtaga attcttggtg
      661 acagacatga acgccgccta cttccagctc accaaacgtg ttctgccaaa tgcgaaagtc
      721 gtgattgatc ggtttcatat tgtcaaacac atgaatcaag cgttcaatga gttgcgtatc
      781 cgtgaaatga atgaacttcg taaagccgga cagaaaagcc aggcagaaaa actgaaaaag
      841 aactggcgct ttttgctaaa aaatcgtgca aacatcaacc attatgaata caaaacatgg
      901 aaaagtttcc gagcaccaaa atacccattt cttactgaag caatgatgat tgatcgattg
      961 cttgagtttt ctccgcccct aaaggaggcg tatccctttt ttcatgaatt agttgaagcc
     1021 tttcgagaca aagaccctga cttatttttc tccttattgg cagaacttcc cgaaacgtta
     1081 gatgacagct ttcgggaaaa gcttcaaaac cttctgacct atgaagaagg catcaccaac
     1141 gcaatgatct atccttattc caatggaaaa atagaagcga agaataccca cataaagaca
     1201 atgaaacgag tatcctacgg atttaaatca tttgagaaca tgagaattag aatctttttg
     1261 atcaatcaat ta
//
LOCUS       DAYYFU010000177         1205 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000177 DAYYFU010000000
VERSION     DAYYFU010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.187"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1205
                     /locus_tag="ACHA34_002973"
     CDS             <1..>1205
                     /locus_tag="ACHA34_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFM8793531.1"
                     /translation="QKKKTLIVDAVFSPAPHTCRNCGSTVVDGNGKVIVVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNAKVV
                     IDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKT
                     WKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFSLLAELP
                     ETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYGFKSFENMR
                     IRIFLINQL"
BASE COUNT          399 a          234 c          232 g          340 t
ORIGIN      
        1 ttcagaagaa aaagacgttg atcgtcgatg ctgttttctc acctgctccc catacctgta
       61 gaaattgtgg ttctactgtg gtagatggaa acgggaaagt aattgtagtg aaaaatggga
      121 agaaggaaac gattgtccgc tttgaacaat acaatcacat gcctttggtt atgcgcttaa
      181 aaaagcagcg ctatacctgt aaaaactgtc gaacccattg gacggctcaa agttattttg
      241 ttcaacccag acattcaatc gcgaatcatg tcagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa attgtcaggt atctttgacc accgttattc
      361 gtacgttgaa agagtttaaa agctacttac caaagcaatc taagaggatt ctccccagag
      421 tattgatggt tgatgaattt cgttcgcacg cttccataga agataaaatg agctttattt
      481 gcgcagatgg cgaaacagga caattaattg atgtcttgcc tacgcgtaaa ttgcctcgat
      541 taacaagcta tttcttaggc tgcgctaatc ctgaagaagt aaaattttta gtcacagaca
      601 tgaacgccgc ctacttccag cttaccaaac gtattctgcc aaatgcgaaa gtagtgattg
      661 atcggtttca tattatcaaa cacatgaatc aagcctttaa tgagttgcgt atccgtgaaa
      721 tgaatgaact tcgtaaagta ggacagaaaa gtcaggcaga aaaactgaaa aagaactggc
      781 gctttttgct aaaaaatcgt gcaaacatca accattatga atacaaaaca tggaaaagtt
      841 ttcgagcacc aaaataccca tttcttacgg aagcgatgat gattgatcga ttgcttggct
      901 tttctacgtc cctgaaggag gcgtatccct attttcatga attagttgaa gcctttcgag
      961 acaaagaccc tgacttattt ttctccttat tggcagaact tcccgaaacg ttagatgacg
     1021 gctttcggga aaagctccaa aaccttctga cctatgaaga aggcatcacc aacgcgatga
     1081 tctatcctta caccaatgga aaaatagaag cgaagaatac ccatataaag acaatgaaac
     1141 gagtatccta cggatttaaa tcatttgaga acatgagaat tagaatcttt ttgatcaatc
     1201 aatta
//
LOCUS       DAYYFU010000178         1193 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000178 DAYYFU010000000
VERSION     DAYYFU010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.189"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACHA34_002974"
     CDS             <1..1076
                     /locus_tag="ACHA34_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8793532.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DAYYFU010000179         1189 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000179 DAYYFU010000000
VERSION     DAYYFU010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.190"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1068
                     /locus_tag="ACHA34_002975"
     CDS             <1..1068
                     /locus_tag="ACHA34_002975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8793533.1"
                     /translation="VKKDGTRFSQEYDKVILATGSKQIMLDIPGNNLANIFSIKNFPN
                     AKEIFKALDDSTIKKVGIIGAGYIGVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFS
                     DKVEEILSKNGIHLCLSESPKKYLGRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIG
                     EKELLRDVSGAYLTNEYQQTNIKDIYAIGDCATTFFTSINRSSVDFSISNALRTAYIA
                     TQHINNQDFTPSGSQFTNGFSIFNYNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFL
                     RKNSRVKLRISFEKNTKRIVGVQLCSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFH
                     PSFSQPYNFILKAVTGLNIET"
BASE COUNT          470 a          143 c          194 g          382 t
ORIGIN      
        1 gtaaaaaaag atggtacaag attctctcaa gaatatgaca aagttatatt agctacaggt
       61 tcaaagcaaa ttatgctaga tataccgggg aataatttag ctaatatttt ttcgataaag
      121 aattttccaa atgcaaaaga gatttttaaa gctttggatg atagtactat taaaaaagta
      181 ggaattattg gagcaggata tattggagtt gaaattgctg aagccataag aaaaagaggg
      241 aaagaagtat atcttttcga tgttgcagat agagtgttgt cgacttatta cgaccgtagt
      301 ttttcagata aagttgaaga aatactatca aaaaatggaa tacatctttg tctatcagaa
      361 agtcctaaaa aatatttagg aagaaaaaag attgaaaaaa tagtcactac aagtggtacc
      421 caatactcta tggatatggt cgtacaagct ataggatttc tatctaatag tccaattggg
      481 gaaaaggaac tcttgagaga tgttagtgga gcatatctaa ctaatgaata ccagcaaaca
      541 aatataaaag atatatatgc tattggggat tgtgctacaa ctttttttac gtccattaat
      601 agaagtagtg ttgatttttc tatttctaat gctctaagaa cagcttatat tgcaactcaa
      661 catattaaca atcaagattt tacaccttct ggttcccaat ttacaaatgg cttttctata
      721 tttaattaca atttttacgc agcaggtttg aatttaaaaa cagcacgatt attaagtgag
      781 tctatagatt atatagaaat agaagatttc attagacctg cttttttgag aaaaaatagt
      841 cgtgtaaaat taagaattag ctttgaaaaa aatacaaaga gaatagtagg tgtacaactt
      901 tgtagtcaag aagatttatc tggactatta tcaatgtttt ctcttgctat agaagaacaa
      961 attacattag acaaattgaa aatgttagat tatttatttc atcctagttt tagccaaccc
     1021 tataatttta ttttaaaggc agtaacagga ttaaacattg aaacataatc gttactaaat
     1081 gaaatcaaga aatatactgt attactcaat tcattgaaaa tatttatcat aatttttaat
     1141 agaaaacact attaaagaga aaaaagagag gtaaatcaaa tgaaaaaat
//
LOCUS       DAYYFU010000180         1187 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000180 DAYYFU010000000
VERSION     DAYYFU010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.191"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..1181
                     /locus_tag="ACHA34_002976"
     CDS             66..1181
                     /locus_tag="ACHA34_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793534.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
BASE COUNT          446 a          177 c          216 g          348 t
ORIGIN      
        1 cttagtcaaa aaattatata agtttgcgta cgattcaaag aaaattaaga gaacttaata
       61 ctattttgaa aaactatcat ttatcttgca gcttgaaaaa aggtaatccg atagaaggag
      121 aagaataccg tatccgatac ttttttcact tgatgtattg gcagtcattc gacgaaaatc
      181 attctgatat attatcaagt ataaaaaata aacgtcaact attagaaaaa tttgcgaaac
      241 atgcgccata tacgcgtagt atagatagaa agaaatggat cagcctacta agaatttcgt
      301 tgcaacgaat gaaacaaggc cactcgatta cgtatgtccc agaagagatg aacaaatttg
      361 tgcatccatt gataacaaaa gaagagttta cgcattacat actagagcct ttttttgaag
      421 caaacttctg gtcgatgaat cacatcactc caacagagca agcttacctt tattttatgt
      481 ttggtgtaat gaacacctat ttggaagaag atttgttgga tactccagat aaagtacaag
      541 aaagttgggg ctggattgaa tcgtctattg tagaaaattt tgaaaaaaac ttcaagattt
      601 gttttacaac ttcagaaaaa aattatctat atgtcaattt ggcaatgatc catttgtatt
      661 caagggtttt tgggacaaaa aagaaggtgg atgcttttgg aaaatcagca gaagacagag
      721 attttcagca tatatttcca gtgatgtatc caatcatgca acagtttttt agtggggtag
      781 ctcgcagaat tccagagctt aatacttatt atgaaaagaa cagacggttg atttttcaat
      841 actgtatgtt agtcagggat atttttataa aacacgaaca acctgtcata ttctatattc
      901 aatcaaagta tggtaaaaca caggaattat gggcaaagaa aaggatatta tctatgactg
      961 aacgcccaat aaaatatagt gcatccgctg atcagttacc tgatattgtc attagcgatt
     1021 atcctatcga caaaaaggca tatgatcaaa cgaatacaca gatattttac tggaatggac
     1081 aacctacaaa aaatgattgg gcgcgtctga aagctgaaat cataaaaatc aggaatgaga
     1141 aaaatagatc catattggaa aaattggatt tcgttaatta agaaaag
//
LOCUS       DAYYFU010000181         1150 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000181 DAYYFU010000000
VERSION     DAYYFU010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.192"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            178..381
                     /locus_tag="ACHA34_002977"
     CDS             178..381
                     /locus_tag="ACHA34_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HFM8793535.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            381..731
                     /locus_tag="ACHA34_002978"
     CDS             381..731
                     /locus_tag="ACHA34_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8793536.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
     gene            complement(766..>1150)
                     /locus_tag="ACHA34_002979"
     CDS             complement(766..>1150)
                     /locus_tag="ACHA34_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFM8793537.1"
                     /translation="ITNPGVHDVKVLETLSEEANLPNILGDKGYIRHKIHEKLALKGI
                     TISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFESI
                     LLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT          412 a          160 c          191 g          387 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttacact ctcgtcaatc
       61 attgttatat aataaaatgt tatataataa aattactaat gatatttaat attacaagca
      121 atcttaagaa attcgaaaag gaaaaaatga ttatgaaaaa gaaagaaaaa gaaagtattg
      181 aaacattgtg ttattctagg aatattagga acttgtctag ctggcattgg tacaggaata
      241 gacgttgacg cagctactta ctatggaaat ggtctttatt gtaacaaaga aaaatgttgg
      301 gtaaattggg gacaatcatg gtcagaaggg ttaaaacgtt ggggagataa tctttttggt
      361 agttttagtg gaggacgata atgacttcaa taaacattga tcacacaaaa aaagtcatac
      421 atgaattata caatagttta atgacacgac ctaatcagac gaaagcatta ttagatatta
      481 ctgatgtttt atttcaggta tataaaaaaa ttgatacagc aaaatatcct gaaattcttg
      541 taaataaatt agttaattat atttacatcg tgggttttga taataaaata cgctttattg
      601 ataatgatga aaaattatta atagaattag gggatatttc taaaaaagct ggaattaata
      661 gtaaatacaa agcaaacttt acagataaat cgcaatttta tagttattca gaaacaatgc
      721 ctcaacgata aaatatagta tactaacccg tggtgctagt taattttaat atcctaaaga
      781 atatctcaaa gttccttcaa aacgtcgttg tgctcgactt aatagaacac tgtaagccag
      841 taatatgctt tcaaatctac tctctagccc tttaacagaa cgtgagttga aattttgaca
      901 acctaatttt tctaaggaag aaaaaacagt ctcaactgtt tttctttgtt ttccaagtaa
      961 gctgtggtct agtttttctg atttatccat gtttttacgt ggcggaacgg atatagtaat
     1021 gcccttaagg gcaagctttt catggatttt atgcctaata tatcctttgt cccctaggat
     1081 attgggaaga ttcgcttctt ccgataaagt ctctaacact ttcacgtcat ggacacctgg
     1141 attcgtgatt
//
LOCUS       DAYYFU010000182         1143 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000182 DAYYFU010000000
VERSION     DAYYFU010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.193"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="ACHA34_002980"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="ACHA34_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFM8793538.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DAYYFU010000183         1116 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000183 DAYYFU010000000
VERSION     DAYYFU010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.194"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..899)
                     /gene="vanY"
                     /locus_tag="ACHA34_002981"
     CDS             complement(<1..899)
                     /gene="vanY"
                     /locus_tag="ACHA34_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFM8793539.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          159 g          401 t
ORIGIN      
        1 ttagtatgtg ttgatccggg aaacactgtc acaattacac cgtctatatt gtttcctgat
       61 atctcataac gaagattagt cggcacatga atggtggtat ttttagtaac aggataataa
      121 aagatctcat atttttcccc atttacacta acagaaatgg ttttttcttc ttttaggtaa
      181 tccatatatt cctcgagaac gaaattcttt tctttcataa tcgcactatg tggtaaacca
      241 acatagcgaa tatgccatgg ttcatattga attcctgtta actctgtttt gtcctctgga
      301 taacgtaaaa tgaacccgta tttccaagca ttttcttcta tccactttcc ttcaggggct
      361 cgttccattt tcgtcaagct tgatcctaca tctagtgata aacctgaatt atgctcacta
      421 taacctgctg gtaaggcata ctcagccccc atttcttggt aaagcacact ttgctcatca
      481 aagtctcgat agccactatt aataataaaa tgactaacgc caccctttac agcatcattg
      541 accatctctg aaaatttttg tgctatttct tttgacatat aaatattact atcaagcaac
      601 ccgtatccat ttattaattc gtcatgttta gataaattca cgatatctga cttcacactt
      661 tcttggcgaa caggatattt actattgatt aatagcagat ttccttgata aacctgttct
      721 tctgtaattg ttttctcttg ggtattttca gaagtcccac tattttctaa atgttctttg
      781 ggattttgat cataattttg aaattcgact ttttcctgag aaaacagtgc ttcattaacg
      841 tagtcataac ctaagtatat taagaataac aataacaata aaaaaaacaa cttcttcatt
      901 ttcagtctcc tccttaacta tatagttgga agtatgaaat atttctagaa tcttcccctt
      961 aaacggcaca atctcgtttg agattgtgcc gttttgcccc gtaaattaca gtcaattatt
     1021 tcgccctact ggatttacta ttatcaccaa tgtagatgaa ttttcataag tgatacccat
     1081 gctgaattta acgaggctga actgcctgtt gacaat
//
LOCUS       DAYYFU010000184         1106 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000184 DAYYFU010000000
VERSION     DAYYFU010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.195"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACHA34_002982"
     CDS             <1..1087
                     /locus_tag="ACHA34_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8793540.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          403 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaa
//
LOCUS       DAYYFU010000185         1074 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000185 DAYYFU010000000
VERSION     DAYYFU010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.196"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            441..1074
                     /locus_tag="ACHA34_002983"
                     /pseudo
     CDS             441..1074
                     /locus_tag="ACHA34_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          372 a          186 c          189 g          327 t
ORIGIN      
        1 tagattactc actatataaa atgtgattaa ttttattatt aataaaatat tttttatatt
       61 acaaaagtag tgtttcaaag ttagtagaga aaatgtagtt atatatttaa atttttacca
      121 gaaagaaatg aacaccttca attatctaaa tctcgaaaat ggttagatgt aatcatcagg
      181 aaaaacaatc ttaaacgaat tactattcat ggtcttagac acacacatgc tagcttgctt
      241 ttagcaagct agtgcaaata ttaaagatgt gcaagagcgt ttaggccact cttctattca
      301 aatcattatg gatctatata ttcatattac agacaaaaga aaagaagaaa ccgtagcgca
      361 attcgcaaaa tataggctct ataatttatg ggactgatga atcaaaattg attcttcagt
      421 cccattgtta ttttaggtat atgattcgta cattgaaaga gttgaaaaac tatcttccga
      481 agccgtcaaa aaggaccctg ccaaaagtat tgatggtgga tgaatttcgt tcgcacgctt
      541 caattgtgga taaaatgagc tttatttgtg cggatggcga gactggaaaa ctcattgata
      601 ttttgcctac acgaaaattg tctcgattaa cacactattt tagtcgctgt gcaaatcctg
      661 aagcggtgga atatttagtg acagatatga acgccgccta cttccaactg acaaaacgtg
      721 tcttaccaaa tgcgaaagtc gtcattgatt gttttcatat catcaaacat ttgaatcagg
      781 cgttcaatga ttttcgtgta caagaaatga aaagattaca ccagttaggg caacgacggg
      841 aagccgagaa agtgaaaaag aactggcgct ttcttctaaa aaaccgtcaa aaaatcaatc
      901 actatgatta caaaacatgg cgcagttttc gtgcacctaa atacccgcta ctcactgaag
      961 caatgatgat tgatcgattg ctggcatttt caacgccttt aaaggacgcc tatacttttt
     1021 tccatgagct gaccgaagcc tttagagaca aggaatcaga cttatttttt acct
//
LOCUS       DAYYFU010000186         1060 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000186 DAYYFU010000000
VERSION     DAYYFU010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.198"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..994)
                     /locus_tag="ACHA34_002984"
     CDS             complement(38..994)
                     /locus_tag="ACHA34_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793541.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          380 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttttctg
      961 tttttataat tataggtagc gtatacaatt ccacaaaact tatctctgtt gagttaaaaa
     1021 gattatataa aaaaagccga atgtttttag gattccctgt
//
LOCUS       DAYYFU010000187         1051 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000187 DAYYFU010000000
VERSION     DAYYFU010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.199"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ACHA34_002985"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ACHA34_002985"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM8793542.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DAYYFU010000188         1035 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000188 DAYYFU010000000
VERSION     DAYYFU010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.201"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1035)
                     /locus_tag="ACHA34_002986"
     rRNA            complement(<1..>1035)
                     /locus_tag="ACHA34_002986"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DAYYFU010000189         1003 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000189 DAYYFU010000000
VERSION     DAYYFU010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.202"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(228..>1003)
                     /gene="menA"
                     /locus_tag="ACHA34_002987"
     CDS             complement(228..>1003)
                     /gene="menA"
                     /locus_tag="ACHA34_002987"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFM8793543.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
BASE COUNT          357 a          206 c          170 g          270 t
ORIGIN      
        1 tctcggtatt aagccaaatt tatgcaaaaa tgtggaaagc cattttcaca aaatttttct
       61 taatactcga aactaagcga ctttttcaca acctcctgat aaacggtgtt caaaagcttg
      121 catctgcggt aataagccca accaaacgaa aaatataagg agctattttt atttggctgt
      181 tcttattact tgatgcaggg cagttttttc acaacctctt catgctgtta aatggcttgc
      241 ataatgattg ttagtaatag ccccaataca taactggcat tgaataagac catatttttg
      301 atggaatagc tgaaactgat aggctggggt aacgtttgct tgaattgatc caaatgcttc
      361 ttcacaattg gcaaggtcaa gaaggtgacc aagatcggcc attcatagat accgctcaac
      421 aaccccatta aaatcacgaa atagcaagca tacatcaata gctggaataa gactgttccc
      481 gcaggacgtc cgatataaaa aactagtgta tagcgatgat tttcaatatc acggtcaaga
      541 tcccgcaaat tattagccaa catgatattg gcaatcgtaa acaccaaggg taaggaagct
      601 aatacgattg ctgctatagc ccaaagattt cctaccaaat caaattgtcc agatgaaaag
      661 ttgactgtca gatcatagac atctgaaccg aatgtattga cataaacgac catggcaaaa
      721 attccaaggc ccatagtcac cccactgaaa atttccccta aaggcattcg agataaagga
      781 atcggtccga atgtatagaa tactccaata aaacaacaga taaatcccat caaaagtaaa
      841 agccaacccg tattgatcgt tagaataacg ccgataacaa aaacaaatgc aatcatagct
      901 aaaatcatgt ttcgaatcaa tacaggagat agatttctgc ggccaatgac attttcttca
      961 tacttatata cttcagaatg agccttttca taatccatgt aat
//
LOCUS       DAYYFU010000190          997 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000190 DAYYFU010000000
VERSION     DAYYFU010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.203"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..913)
                     /locus_tag="ACHA34_002988"
     CDS             complement(26..913)
                     /locus_tag="ACHA34_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8793544.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          323 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaatt
//
LOCUS       DAYYFU010000191          986 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000191 DAYYFU010000000
VERSION     DAYYFU010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.204"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..704
                     /locus_tag="ACHA34_002989"
     CDS             <1..704
                     /locus_tag="ACHA34_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8793545.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          333 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataat
//
LOCUS       DAYYFU010000192          959 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000192 DAYYFU010000000
VERSION     DAYYFU010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.205"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..511)
                     /locus_tag="ACHA34_002990"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="ACHA34_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="ACHA34_002991"
     CDS             complement(570..>959)
                     /locus_tag="ACHA34_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8793546.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DAYYFU010000193          958 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000193 DAYYFU010000000
VERSION     DAYYFU010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.206"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..644
                     /locus_tag="ACHA34_002992"
     CDS             <1..644
                     /locus_tag="ACHA34_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8793547.1"
                     /translation="VIFSGKQYADNILLWGGEDNIEKINNNLTILDTALENKEQKISI
                     LNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKTNLENQLSAKDSELQGKQ
                     NEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQSTINSLNQQINQMLSEKNN
                     IQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTANKAQQIVNEHTIQ"
BASE COUNT          423 a          117 c          137 g          281 t
ORIGIN      
        1 tagtaatttt ctccggaaag caatatgctg ataatatttt attatgggga ggggaagata
       61 atattgaaaa gatcaacaac aatttaacta tattagacac agctctagaa aataaagagc
      121 aaaaaattag tattttaaat tcacaattgt cttcaaacaa ccaaactctt gaacaactaa
      181 gaactaatat tgaagtttcc aaacaaaaag tggcatcttt agaaaacgac aagaatcaat
      241 tagtatcaga gaaaactaat ttagaaaatc aattgtcagc aaaagactct gaattgcaag
      301 gaaaacaaaa tgaaattaat gcgaaaatag acgaaattaa ccaaaaagta gctgaaataa
      361 atcgattaaa tagcgattgg aataatcgtt tacaaaatga aaagaacaaa tttaatgaag
      421 aacaatctac tattaatagt ttaaaccagc aaattaatca aatgttgagt gaaaaaaata
      481 acattcaaaa tcaactgcaa gctactcagc aaaagctaga agaagaaaag tcaaaaaata
      541 gtagtttgaa taattacatt gaaaagcttc aaaaagctaa aaatgatgta gaggataccg
      601 caaataaagc acaacaaatt gtaaatgaac atacaatcca atagatatag ttttaattta
      661 acaaatggta tgtttggaga taatgagata gaatataaat gtcataatat tatacaatct
      721 aaacaccgtt tccttttcta tttttgatga catcagtata tagctactcg aaagagtagt
      781 ctttttatta ttctcgctta ttattgatat gaaaatttcg atttgacaaa aggtaccgaa
      841 taattttgta atatgaatta ttatcaagaa gtaattatga tgataacata tggaaactag
      901 ttagtaataa taacaggtga ttaaactttt gaacgacttg taattctata ttttttct
//
LOCUS       DAYYFU010000194          931 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000194 DAYYFU010000000
VERSION     DAYYFU010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.208"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>931
                     /locus_tag="ACHA34_002993"
     CDS             <1..>931
                     /locus_tag="ACHA34_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8793548.1"
                     /translation="HCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFY
                     RPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFS
                     RFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETT
                     VRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAA
                     CPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNA
                     LHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          286 a          190 c          180 g          275 t
ORIGIN      
        1 acactgtttc attagtcaaa aagttcgttg gtctgtcatt gctcgcttaa aagagaatac
       61 ttccatgacg gaaatcgctc gtcagaaaaa tatttccact tcttcggtct atcgtgtcat
      121 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
      181 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      241 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      301 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      361 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      421 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      481 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      541 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      601 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      661 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      721 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      781 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      841 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      901 gtacggcttt cgcaactttt acaatttcaa a
//
LOCUS       DAYYFU010000195          907 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000195 DAYYFU010000000
VERSION     DAYYFU010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.209"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            103..907
                     /locus_tag="ACHA34_002994"
                     /pseudo
     CDS             103..907
                     /locus_tag="ACHA34_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator BglG"
BASE COUNT          311 a          143 c          155 g          298 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggat
//
LOCUS       DAYYFU010000196          904 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000196 DAYYFU010000000
VERSION     DAYYFU010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.210"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            265..888
                     /locus_tag="ACHA34_002995"
     CDS             265..888
                     /locus_tag="ACHA34_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8793549.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          333 a          129 c          167 g          275 t
ORIGIN      
        1 tagttttaaa ataaactaat gtccttattt ttgtgttcac atttctgtta cctaagaaat
       61 acttaaagga ggtgatgtag ccagcaacga ctacaacttg aacccgctat ctacagttta
      121 tttgttgttt cttttgggta tactctaatg gtacaaatcc atatttgctt aaagcgttga
      181 tttttcagcg gtttttcttt attgcaaagt gtaccctaag acctgtatac taatagtaaa
      241 tagattggat aggaatgatg gcaaatgtat ttagcttatt taagagtttc ttccgacgat
      301 caaagtttag ctcggcaaca taagctaatt gaaaattggt cagtcaaaaa taatattgct
      361 gaagacgatt taaaaatttt cgaggaaaaa gtctctggaa aaaatattga aaacagacca
      421 gagctgcaag agcttatttc tttcatacgt gaacgagata cgattattat tcctagtctt
      481 gatcgattag caagaaatag taaagataca aaagacttac ttcaattgtt tcgtagtaaa
      541 ggtgcttcta ttgaaatatt agacctaccg tcgtttaatg gggtaactga ccctgcatta
      601 agagacttgc taacaaattt agtcatagaa gtgtttagct atgttgctga aaacgaacgg
      661 aaaaaaatca aagagcgtca aaaacaaggc attgaaattg caaagaaaaa tggtcgttat
      721 actggtaaaa agattcaata ccatgccaat gcaacaggaa aaaacaaatt gatttacgat
      781 gaaattgttg atggtctgaa gaaaaaagaa actatttcta aaatagcaaa gaacgcagga
      841 gttacaagaa aaaccgttta tcgagtaaaa agagatttag gaatgtaata tgttccagtt
      901 agaa
//
LOCUS       DAYYFU010000197          888 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000197 DAYYFU010000000
VERSION     DAYYFU010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.211"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..358
                     /locus_tag="ACHA34_002996"
     CDS             <1..358
                     /locus_tag="ACHA34_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFM8793550.1"
                     /translation="FFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRRFPEGEE
                     VWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDDWQKVMA
                     AEVIATYLDGDLVYKK"
     gene            406..>888
                     /locus_tag="ACHA34_002997"
     CDS             406..>888
                     /locus_tag="ACHA34_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM8793551.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     D"
BASE COUNT          261 a          148 c          205 g          274 t
ORIGIN      
        1 cttctttaat tattgtcgat tagaggaaag tggtgtaaca gtcactagcg gtacagattt
       61 accgctgttt attactgata ttcctgaatc gattataaga gcagtattcc gtcgttttcc
      121 tgaaggagaa gaagtctggg agaaagaaac aagcttcacg acaaccgagc tgctgaaaag
      181 ttttacaagc aacagcttca aagtaaatgg tttatcggag tacggagaaa taaaagtagg
      241 gaaaagcgcc agcttagctg ttttcagcga ggacctctgt actgatgact ggcaaaaagt
      301 gatggctgcg gaagttatcg ctacctatct tgatggtgac ttagtttaca agaaataatg
      361 cttttcgata taacgttata aaacgaatta aagagaggtt aaaaaatgga aacaatgaaa
      421 agaaaattca ccttcccaca tagttataca ttgattttta tcttaattat tttggctgca
      481 attttaactt ggataattcc tagtggtcag tttgagacag ttgttgaaaa cgtcgcaggt
      541 atggaaaaaa caacggttgt tccgggaacc tatcaccaaa tcgataaggc agattatgga
      601 caaggttttt cctctatact aatggcacca gctgaaggga ttatttcagc tgttgaggtt
      661 gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta tcctaaaaac gggagccatc
      721 gatcgagggc tgtttacctt agcaaaagtt ctgggagaca aggggatctt agttattccg
      781 atgtcgatga ttctattcag cttaggagga tcaacctttg gcatgagcga ggaagtcatt
      841 cccttatttg cggtgtttat ttctctgatg ttttctcttg gctatgat
//
LOCUS       DAYYFU010000198          849 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000198 DAYYFU010000000
VERSION     DAYYFU010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.212"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..>849
                     /locus_tag="ACHA34_002998"
     CDS             76..>849
                     /locus_tag="ACHA34_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HFM8793552.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNFHLDSTHIKANA
                     NKHNFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEQEKQFAYSAHTSCDDNGFILSTIITPGNIHDS"
BASE COUNT          280 a          158 c          165 g          246 t
ORIGIN      
        1 gccagactgt agacaaagtc cattttatgg atgatgtcta cagtcttttc ttttataata
       61 gatttatgaa aacgaatgca aggagtgata gctatgatgt ctaaaaacaa tacgaatggt
      121 agaaatcaat ttgcgatgct tacgattgat gatctagttc ctcaagatca tctcgttcga
      181 aagattgatg ccgcgcttga ttttgaattt atttatccaa tcgtcgaagc cacttactcc
      241 gatttaggtc gcccaagtat cgacccagtg attctgatca aattggtgtt tattcagtat
      301 ttattcggta tccgttccat gcgacaaacc attaaggaag tcgataccaa tgtcgcttac
      361 cgctggtttt taggctactc cttcgaagag aaaatccccc atttctcaac ttttggaaag
      421 aattatgttc gccgatttag agaaacgacg gtgtttgagg atatttttgc ctatatttta
      481 gaacaagcag ttaaagcagg ttttgtaaca gaggataatt ttcatctaga ttctacccac
      541 atcaaagcta acgccaataa acataatttt acgaaagaaa tgacccatga tgaagcgaaa
      601 gcctatcagg atgaattaga agatgagatc aatcgtcaac gaatagaggc tggaaaacgc
      661 ccttttactt tggacttaga aaaggaagtg aagctgaaag aaagaaaaat aagtaaagct
      721 gatccggaaa gcggctacta cgtcaaaggc gaacaagaaa aacaatttgc ttattctgcc
      781 catacttcat gcgacgacaa tggatttatt cttagcacaa tcatcactcc tggaaatatt
      841 catgacagt
//
LOCUS       DAYYFU010000199          822 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000199 DAYYFU010000000
VERSION     DAYYFU010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.213"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..508)
                     /locus_tag="ACHA34_002999"
     CDS             complement(<1..508)
                     /locus_tag="ACHA34_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793553.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRE"
BASE COUNT          263 a          136 c          123 g          300 t
ORIGIN      
        1 tttcacgtac acgagcgaca tctgcacttg tcaccttttc tttgatgatg acatcgtcta
       61 cagcttgctg ccagtttgat acatttttag taaataaatc gttgacagct gtctggatct
      121 tcagttttga ttctgcacta tctaactgtg tcacgattgc tttcttctca tcttggattg
      181 caacagcttc tttaggcaat tctgattctt tgatgtcgta atcatctgct gaaagtttga
      241 cagaggatag cttatttgac aaacttgtga ttgtttcttc tgttatttca gggtttagaa
      301 aaattttttc ttttgtacta tacagcttgt ctacttcttt tgataaagaa gtcaaggtgt
      361 ctttttgatt ggtgatggca gattctgcat aattgatccg ttgctgatgt tgccgctgat
      421 tattgatatg gtatccaaac aaacagatcg caatagcacc tgcacctacc gccacagcag
      481 tcaatgactt gacgaatggc ttttccatag attcactcct aagtattgat gatagataaa
      541 ttaagatata attcacttac ataaaaatga cagcttttta tttaaaaaaa cataattaaa
      601 ctaatatatt cacagaaata tttgtaaaca agttttccct atataatcat accacaaact
      661 gttttcttga ctagggcaat ttacgattta aaagtaaata aaacattaat aagataaaaa
      721 ttaatcgatt tcatttttat ttccataatt ttcataggtg tttttaaaag ttacacaaaa
      781 aatagaataa caaaaaaatt aacattgaat taataatagt ta
//
LOCUS       DAYYFU010000200          811 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000200 DAYYFU010000000
VERSION     DAYYFU010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.214"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..672
                     /locus_tag="ACHA34_003000"
     CDS             <1..672
                     /locus_tag="ACHA34_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8793554.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAYYFU010000201          807 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000201 DAYYFU010000000
VERSION     DAYYFU010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.215"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..749
                     /locus_tag="ACHA34_003001"
     CDS             21..749
                     /locus_tag="ACHA34_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8793555.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
BASE COUNT          297 a          145 c          137 g          228 t
ORIGIN      
        1 taaaataaag gagtaatgat atgattccca tttatatttg tgaagacgat ccaaaacaat
       61 tagaagtttt atcaacggtg atcaaaaata gaatcatgat tgaaaactta gacagttatg
      121 tccatatttc tacctctgat ccggaagaac ttctcaatgc agccaagatc agaacttctt
      181 cttttggtat ttacttttta gatatcgaac tagaagacag tgaaatggat ggtgtagcga
      241 tcggtaaatt gatccgtgaa ttagatcctt taggaaaaat aatctatgtg acttcccata
      301 cggaaatgtc gatgaaaatc ttaaaaagca acatcgaacc gactgattat atcgtcaaag
      361 aagatcttta tgatttaaaa gaaaatgtcg agcatatcct gagcaaaatt ttcgaagatt
      421 ttcaaatgtc ccaaatccaa aaagatatct tcaaaataga gtttaatgat gagatcaaat
      481 ttttaccgat caaagacatc caatattttt ctacttcgct aggagcgcca cataaactgg
      541 aagtccattt gactcatgcc caattacaat tctatgagaa gatcaaagaa atcgagaaaa
      601 tgcatcggaa ttttatccgt tgtcataaat cctatgttat caatacacaa aatgtgcggt
      661 cgattaataa aaaaaccaga gaagtcacgt tggcaaacgg cgagaccatc ccagcctcta
      721 tccgtggatt aaagaaattg atttcataaa aagaaccgaa tagtttggac cgactaaatg
      781 accagccgat ccaaaccctt cggttct
//
LOCUS       DAYYFU010000202          803 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000202 DAYYFU010000000
VERSION     DAYYFU010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.216"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..>803
                     /locus_tag="ACHA34_003002"
     CDS             129..>803
                     /locus_tag="ACHA34_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM8793556.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMNA"
BASE COUNT          240 a          155 c          147 g          261 t
ORIGIN      
        1 gctctataat ttctaggact gatggaaatt ttctatctgt cctagttttt ttgtaaaaaa
       61 acaaaaagac atacaaaatt tccggtagaa tagagtctcc tacaacatca ttctggggga
      121 attcttgtat gtccatgaat cattttatca aacaactctt tactattaaa gactcaaata
      181 tccaaattca atcctttgaa gaaacttttt ataaaagcag aaaaagtatc gttattgagg
      241 gaactctgat ccgtacctac agacgttgtc cgtgttgtaa agattctaca aagcaaatcg
      301 ttaaaaatgg caagaagatc tctatgattc tcctgaatcg tagcggtaat aaacggacct
      361 atcttcggtt gaagaaacag cgatacctct gccgagcatg taaaaaatat tttaccgctc
      421 ggacgtatct cgttacccca ttctgcttta tttctaagca gattcattac aagatcttag
      481 aagaattgac agagcgtcaa tctataaaag caattggtaa acattgcgat gtttctgtga
      541 caactgttca gcggacattg tccgccttgt catcagatat ccgtcagaat cttgagtatt
      601 tacctcggtg tctattattc gatgagtttc gttctctttc tacatggcac ggtaaatttt
      661 ctttttcttg tatggacggt gagaccggga aactgtttga gatcttacct agccgccgta
      721 aaaaagatct cgttgctttt ttcatgcgtt tttctctcaa agcgaggttg ggtgtacatt
      781 acatcgtaac ggatatgaac gct
//
LOCUS       DAYYFU010000203          782 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000203 DAYYFU010000000
VERSION     DAYYFU010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.217"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..111)
                     /locus_tag="ACHA34_003003"
     tRNA            complement(39..111)
                     /locus_tag="ACHA34_003003"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(76..78),aa:His,seq:gtg)
     gene            complement(114..187)
                     /locus_tag="ACHA34_003004"
     tRNA            complement(114..187)
                     /locus_tag="ACHA34_003004"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(153..155),aa:Trp,seq:cca)
     gene            complement(194..274)
                     /locus_tag="ACHA34_003005"
     tRNA            complement(194..274)
                     /locus_tag="ACHA34_003005"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(238..240),aa:Tyr,seq:gta)
     gene            complement(276..351)
                     /locus_tag="ACHA34_003006"
     tRNA            complement(276..351)
                     /locus_tag="ACHA34_003006"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(316..318),aa:Phe,seq:gaa)
     gene            complement(387..459)
                     /locus_tag="ACHA34_003007"
     tRNA            complement(387..459)
                     /locus_tag="ACHA34_003007"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
     gene            complement(469..541)
                     /locus_tag="ACHA34_003008"
     tRNA            complement(469..541)
                     /locus_tag="ACHA34_003008"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(506..508),aa:Val,seq:tac)
     gene            complement(557..628)
                     /locus_tag="ACHA34_003009"
     tRNA            complement(557..628)
                     /locus_tag="ACHA34_003009"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(593..595),aa:Glu,seq:ttc)
     gene            complement(643..732)
                     /locus_tag="ACHA34_003010"
     tRNA            complement(643..732)
                     /locus_tag="ACHA34_003010"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(696..698),aa:Ser,seq:gga)
BASE COUNT          214 a          216 c          180 g          172 t
ORIGIN      
        1 cttgccttac cgcttggcta taccccaaga ataaaaaggg cgaatgatgg gaatcgaacc
       61 cacgaatgcc ggagccacaa tccggtgcgt taaccacttc gccacattcg ccatggcagg
      121 ggcagcagga attgaaccca cactaacggt tttggagacc gctgttctac ctttaaacta
      181 tgcccctata aaatggagga gagtggattc gaaccactga accctaagga acggatttac
      241 agtccgtcgc gtttagccac ttcgctactc ctccatggtg gcgcgggaca gaatcgaact
      301 gccgacacac ggagcttcaa tccgttgctc taccaactga gctaccgcgc catattttaa
      361 aattattaat tgaataacaa gaacgacggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca gttcattatg gaggttaacg
      481 ggatcgaacc gctgaccctc tgcttgtaag gcagatgctc tcccagctga gctaaacctc
      541 caacattcaa ttaaaatgac ccgtacggga ttcgaacccg tgttaccgcc gtgaaagggc
      601 ggtgtcttaa ccgcttgacc aacgggccag gtaaatttgc tacggagagt aagggattcg
      661 aacccttgag acagtttggc accatctaca tgatttccaa tcatgctcct tcggcctctc
      721 ggacaactct ccagagtgaa agaagctcaa tactctttct caaaatgaga actccggcag
      781 ta
//
LOCUS       DAYYFU010000204          770 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000204 DAYYFU010000000
VERSION     DAYYFU010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.218"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..>770)
                     /locus_tag="ACHA34_003011"
     CDS             complement(109..>770)
                     /locus_tag="ACHA34_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFM8793557.1"
                     /translation="RIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQ
                     PLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLT
                     DVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQY
                     QFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEA
                     A"
BASE COUNT          213 a          177 c          148 g          232 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccgattcggc
//
LOCUS       DAYYFU010000205          736 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000205 DAYYFU010000000
VERSION     DAYYFU010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.219"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..734
                     /locus_tag="ACHA34_003012"
     CDS             <1..734
                     /locus_tag="ACHA34_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFM8793558.1"
                     /translation="DKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRK
                     LMSQMGLTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKI
                     YLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQ
                     YQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIR
                     WYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          318 a           94 c          122 g          202 t
ORIGIN      
        1 cagataaata tagcaagata aagcaagaaa ttacagcgat tgtaaaagaa tcaagaaact
       61 cttatggtta tcgtagagtc actttagcgt taaagatgaa aggatataca atcaatcata
      121 aaacagttag aaaattaatg tcccaaatgg gacttacctg tcaaatccga ataaagcgat
      181 ataaatctta taaaggaaca gtaggaaaaa ttgccaagaa tgtgttaaaa cgaaattttt
      241 cagtagatac acccaataaa aaatgggtga cagacgtcac tgaattcaag ataaaaggaa
      301 gaaaaatcta tctatctcct atacttgatt tatttaacgg agaaataatt agttatagta
      361 tatcaactag tccaacatat aaactgattg aagaaatgct tcaacaagca attaaaaaaa
      421 agggaactga gggttcactg attctacatt cagatcaagg atggcagtat cagatgccac
      481 aatatcaaaa aaaattaaaa gagaataaca ttattcaaag catgtctaga aaaggaaatt
      541 gcttagataa ttctgtaata gaaaattttt ttggtgtgct taaatcagag tttttttatc
      601 gagaaaaatt ccgatcaatt gagatatttc aaagtaaatt aaatgagtat attaggtggt
      661 ataataacaa aaggataaaa ctaaagttaa atggactatc tccagtagaa tatcggaaac
      721 agtccattaa atagtt
//
LOCUS       DAYYFU010000206          733 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000206 DAYYFU010000000
VERSION     DAYYFU010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.220"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>733
                     /locus_tag="ACHA34_003013"
     rRNA            <1..>733
                     /locus_tag="ACHA34_003013"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DAYYFU010000207          703 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000207 DAYYFU010000000
VERSION     DAYYFU010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.221"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          297 a          106 c          122 g          178 t
ORIGIN      
        1 attccttagg cagcttttgc tgtgtagaaa ggaataaata ataacgaaag ttcaaaagct
       61 aatgccgact attctagaca ttaaagaaga cgaaaacgaa cgataaagaa agaaaagtta
      121 tgaataatcg tttattgaaa aaaacttgaa aaaatacaaa aaattcgttg accaagctaa
      181 atttagttgg tatattatta cttgtccaaa agacagaaga aaatatttat tcaaaaacat
      241 aacaaaaaag ttattgacat gttactaacg acatgataag ataactgagt cgttaaaaaa
      301 gaaccacttg cgaagcttga ttctatcgaa aaaaacttca aaaaagttct tgacaaataa
      361 caaacgataa gttatgataa atgagttgcc gaaagatttc gaaaaacgaa atcgaaaaaa
      421 acttttaaaa aagagttgac aatcgcttgt ctctctgata tgatataaaa gttgctacgg
      481 cagccaaaaa tgaatcggga aacactctcg atgaagtaga cctttgaaaa ctgaacaaag
      541 taagacaaac caaatgtgta gggcgtcttg attcaattca agacaacaaa catttttaac
      601 aagcaagcaa tatgctagca aacaaattga gcttaacaat cgcaagattg ttcgaacttt
      661 ttatgagagt ttgatcctgg ctcaggacga acgctggcgg cgt
//
LOCUS       DAYYFU010000208          702 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000208 DAYYFU010000000
VERSION     DAYYFU010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.222"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..436)
                     /locus_tag="ACHA34_003014"
     CDS             complement(<1..436)
                     /locus_tag="ACHA34_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8793559.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFE"
BASE COUNT          214 a          120 c           95 g          273 t
ORIGIN      
        1 tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat actttataag
       61 ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata tatttttcct
      121 gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga gataacagag
      181 ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg acggttactc
      241 gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg atcgtttttt
      301 tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac tctgataaat
      361 ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct ttgcttaaaa
      421 aaaactcata attcattagt catcagctct ttctacgtat attactttcc atgcattcac
      481 caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca gcgattcttg
      541 tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt tttatttggc
      601 ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc atttttatta
      661 ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DAYYFU010000209          688 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000209 DAYYFU010000000
VERSION     DAYYFU010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.223"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(275..466)
                     /locus_tag="ACHA34_003015"
     CDS             complement(275..466)
                     /locus_tag="ACHA34_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793560.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT          238 a          128 c           92 g          230 t
ORIGIN      
        1 gaaccggggg ctttattcct gcagtccggc tacgccttcc tgcaatagac aaaactccag
       61 atcgggtaat tcgcttgtgg cgaaacccga cctggagttt gtctctgtat tttgaaccct
      121 aaaaattaat caaacagact gatattctca taatcagcgc gaaataagcc attaagtaca
      181 gttttcctac ctatttttga taacccgctg gttagttaat attccttttc cgagagagat
      241 aaatgattgg ttaaatctat atacatctca acctttaaaa atgttttcga aatacttcat
      301 cagtacatgt catacaaatt accagtgcat tctcaaattc tagttcttta attgatttca
      361 gttgaggagc agttactttt ggcggaaatt tccatttttg tccaaacctc ttcccccgat
      421 gaaatttctt tgttgcaatt attgcaatga aaaatttttg tttcaaaaat atacataatt
      481 acttcctcca ataaaaacta tctacctctc aaattatttt tctctaataa gaaaaacaga
      541 aaaggtaata agatacaaca gtgatgattc ctaataaaga aaagactaca ataatatttt
      601 tctaacttat cgccttcaaa gaaatagttt cttatttttc actaaaataa aaaatttatt
      661 gcaaaaaaaa tagaagtgac aaaattat
//
LOCUS       DAYYFU010000210          668 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000210 DAYYFU010000000
VERSION     DAYYFU010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.224"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..>668
                     /locus_tag="ACHA34_003016"
     CDS             51..>668
                     /locus_tag="ACHA34_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFM8793561.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKE"
BASE COUNT          247 a          110 c          132 g          179 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      541 atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat cgaagtttac
      601 gcactgcctc taccacgatt aaaggcatgg aagccattcg aggattatat aagaaaaccc
      661 gaaaagaa
//
LOCUS       DAYYFU010000211          661 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000211 DAYYFU010000000
VERSION     DAYYFU010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.225"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(159..>661)
                     /locus_tag="ACHA34_003017"
     CDS             complement(159..>661)
                     /locus_tag="ACHA34_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HFM8793562.1"
                     /translation="GIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGVLVILEGGMII
                     DTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVMAPLGDLMNVS
                     RDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFAILTLVSAVLL
                     YIATLF"
BASE COUNT          241 a          132 c          117 g          171 t
ORIGIN      
        1 atgcggagca cctccccaaa gttcaacaga agaaatacca attttctgtt gataagcgaa
       61 aaattctttg agagaataaa ataaatagtg gatattcatt cctgctaatt gatttatttt
      121 aatcataact attgcctcct attaatggat gattaagact aaaagagtgt agcaatgtat
      181 aacaaaacag cagaaacaag ggttaagatg gcaaatattt tgaagccaac cttaaaccat
      241 ttagagaaat taatctctgc gatggctaat cctgccagta atacaccact agttggggag
      301 ataatattca ttaaaccatt tccaaactgg ttacttagaa caacaacatc acgacttaca
      361 ttcattagat cacccaatgg tgccataacc ggcatcgtta atgccgcagc tcctgaggag
      421 gatggaacaa aaaaggaaac aattacttga gaaatgagta gaatcgtagt gaacaggaat
      481 tttggaagac cacctaaaag gttagccaag ctgtttagaa tagtgtcaat aatcattcca
      541 ccttccaaga ttaccaagac tcctctagct agcccaatga ttacagcagc ataggcgaaa
      601 tctgaacagc cttcgacaaa agcttcagca atttcctttt gagaaaatct agcgatgccc
      661 c
//
LOCUS       DAYYFU010000212          649 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000212 DAYYFU010000000
VERSION     DAYYFU010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.226"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>649
                     /locus_tag="ACHA34_003018"
     CDS             <1..>649
                     /locus_tag="ACHA34_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8793563.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSV"
BASE COUNT          213 a          111 c          134 g          191 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtag
//
LOCUS       DAYYFU010000213          607 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000213 DAYYFU010000000
VERSION     DAYYFU010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.228"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            145..543
                     /gene="spxA"
                     /locus_tag="ACHA34_003019"
     CDS             145..543
                     /gene="spxA"
                     /locus_tag="ACHA34_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFM8793564.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          212 a           93 c          120 g          182 t
ORIGIN      
        1 tagataactt tcattttttt aaaaaaatta tgaaaaaaag gtagctattt aaaagttgat
       61 tatgtataat agaaacagat gggagattat tatgatcttc tgttacaaaa aagtgattct
      121 tatcacgaaa aggagtggtg cacgttgtta acattatata cttccccaag ttgtacttcc
      181 tgcagaaagg ctcgtgcgtg gttgcaagaa catcagattc catttgttga acgtaatatt
      241 ttttcagaac cattgaacag ttctgaatta aaagcgattt tacaaatgac agaagacgga
      301 acagaagaaa tcatctcgac acgttccaaa gtattccaaa aactgaacat ggatttagat
      361 gatctgccgt tacaagaatt attggaactt gtgcaaaaca atcctggatt gcttcgccgt
      421 ccaatcatga ttgatgataa acgtctgcaa gttggtttta atgaagatga aattcgtcgg
      481 ttcctaccgc gcgatgtacg acaattagaa ttacgtcaag cacaattgat ggctggatta
      541 tagaaggata atacgaaact tggaaaagac tggctctttg tcaaatgatg ttggtaaaga
      601 gaaatct
//
LOCUS       DAYYFU010000214          588 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000214 DAYYFU010000000
VERSION     DAYYFU010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.229"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..150)
                     /locus_tag="ACHA34_003020"
     tRNA            complement(65..150)
                     /locus_tag="ACHA34_003020"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="ACHA34_003021"
     tRNA            complement(167..238)
                     /locus_tag="ACHA34_003021"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="ACHA34_003022"
     tRNA            complement(250..322)
                     /locus_tag="ACHA34_003022"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="ACHA34_003023"
     tRNA            complement(340..421)
                     /locus_tag="ACHA34_003023"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="ACHA34_003024"
     tRNA            complement(429..501)
                     /locus_tag="ACHA34_003024"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="ACHA34_003025"
     tRNA            complement(505..577)
                     /locus_tag="ACHA34_003025"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAYYFU010000215          562 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000215 DAYYFU010000000
VERSION     DAYYFU010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.230"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..247)
                     /locus_tag="ACHA34_003026"
                     /pseudo
     CDS             complement(1..247)
                     /locus_tag="ACHA34_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          165 a          108 c          118 g          171 t
ORIGIN      
        1 attcctcgaa acagttataa actttcccgt gaaaaatctc ttgttttaat aagccaaaaa
       61 aattctccat aagtgaatta tctaagcagt tccctttccg agacatgctt tgaaagattt
      121 tgtactcctt caatttgttt ctataagctt tcatttgata cgaccaacct tgatctgagt
      181 gaaacgtacg acgataggga caatcgttag tggtctggat agcttcctcc aaaccttcca
      241 ttaccgcacg tgtttctgaa tggtggtagc gaccgatttg aacggctagg gcggaaatac
      301 ggtgcaaatg cacggtggtt tcatgcaatg cttacagggc gtattttgta agggtatgta
      361 acggagataa tcccgacggt aatggatgtg agaggccaag ccgacgctga cggtagggca
      421 aacaaacata gttgcactct agcgatcgtt atttattttt aacggtcaaa ctagggtaca
      481 actatgctta aaacacttcg cctcaacttc tggtagcaaa agtgattttg aaagtcaaaa
      541 tattttttat gagttattta ta
//
LOCUS       DAYYFU010000216          544 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000216 DAYYFU010000000
VERSION     DAYYFU010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.231"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..360
                     /locus_tag="ACHA34_003027"
     CDS             <1..360
                     /locus_tag="ACHA34_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM8793565.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          202 a           89 c          116 g          137 t
ORIGIN      
        1 cgaaaccaac cagttgttaa accaaatgca atgcaaatca ataactcatt gaagcgatgc
       61 tttgtgaaag cattggctct acacggactg ggattatatg tatttcaagg ggaagatatt
      121 ccaacaccac ctagaatcga tacaaagaaa ttaaacatgc tagagacgat tctagaagct
      181 ttcaatgagc agatgggtaa agatatgacc aaaaccttaa tcgaatatgt taatgagcag
      241 acagataaat tagggctctt agctgataac gttgaaacta ttgaacagtt aagctatgag
      301 caatgtgcct tgatggagcg agcaatagca gctaagagaa aagaattaga taagaagtga
      361 tatgagtgtt taaaccatta atcgattcat attcagcggt tctgaaaaag ttcaaaggaa
      421 aagacataag cgcaaccatc aatgaggaag tgaacattga tcgactaaag acgatgtatg
      481 acggctacga tggtgatcga gtcattgaaa ttcgttttat tgatcctaga cgtttcaccg
      541 taca
//
LOCUS       DAYYFU010000217          526 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000217 DAYYFU010000000
VERSION     DAYYFU010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.234"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ACHA34_003028"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ACHA34_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM8793566.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DAYYFU010000218          510 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000218 DAYYFU010000000
VERSION     DAYYFU010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.235"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..510
                     /locus_tag="ACHA34_003029"
                     /pseudo
     CDS             1..510
                     /locus_tag="ACHA34_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          212 a           88 c           92 g          118 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag
//
LOCUS       DAYYFU010000219          502 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000219 DAYYFU010000000
VERSION     DAYYFU010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.236"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            338..>502
                     /locus_tag="ACHA34_003030"
     CDS             338..>502
                     /locus_tag="ACHA34_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFM8793567.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIAC"
BASE COUNT          177 a           89 c           74 g          162 t
ORIGIN      
        1 tctgtactga aatcaaggta ttattgggaa tcccagctta aatcatagat accgtaaggg
       61 attttattct ttatttaaaa ctttgcaaca gaaccttttg ccatatctac tccccctcta
      121 atttatatct ccattctaac gtacttaatt aagtacgtaa attcgattgt tttgtttaaa
      181 ttggagtacc aaaacagcat tgtaaagtgt tatccgtttc ctttataccc gtggtgctag
      241 ttaatttttt cctagaggaa aatgtctaaa catgttggta aatcaacaaa aaaacgcata
      301 gagtagtctc aaaactccca agaaatgagg actacatatg cgttcatcaa aatatactga
      361 acattataca gatactttta taacattcaa ggaaattatg agaactgttt ctaacatata
      421 ccacaattgt gtaccagata agattaaaaa ccgaagaaat actgatcaac tgaagcaacg
      481 cgatacagtg attattgctt gt
//
LOCUS       DAYYFU010000220          491 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000220 DAYYFU010000000
VERSION     DAYYFU010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.237"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(108..>491)
                     /locus_tag="ACHA34_003031"
     CDS             complement(108..>491)
                     /locus_tag="ACHA34_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8793568.1"
                     /translation="AFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHK
                     GCPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPV
                     GYRNQRLAQRILDNELGCANASEAV"
BASE COUNT          142 a          106 c           96 g          147 t
ORIGIN      
        1 gacaaatttt ataaggtgca gaacttcttt ccgtatgcta ttccgattgt ccttgacaat
       61 gagcctcctc ggatgtgacg atcttcggca ggagctaaca gttaaagtta gactgcctcg
      121 ctagcgttag cacatccgag ctcattatca aggattcgct gcgccaatct ctggttacgg
      181 taaccaaccg gtgtctcgta gccaagtgta ccgtgcaacc gaaggtggtt ccaccaattg
      241 acatagtcaa ataactccaa atccaattgt tgtaaggttt caaatgtgta ttgatagaca
      301 aattctactt tcaacgactt ataagttgat tcagctacgg cattatcaaa aggacagcct
      361 ttatgactca atgatcgatt gatgtcaaaa gttgttaata attcatcaat agcttggtta
      421 tcaaactctt ttccacgatc agtatgaaaa atctcaacct ctgtcagagg ttgtttgata
      481 cggctaaatg c
//
LOCUS       DAYYFU010000221          452 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000221 DAYYFU010000000
VERSION     DAYYFU010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.238"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>452
                     /locus_tag="ACHA34_003032"
     CDS             <1..>452
                     /locus_tag="ACHA34_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793569.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGST"
BASE COUNT          165 a           68 c           92 g          127 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca ac
//
LOCUS       DAYYFU010000222          451 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000222 DAYYFU010000000
VERSION     DAYYFU010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.239"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>451
                     /locus_tag="ACHA34_003033"
     CDS             <1..>451
                     /locus_tag="ACHA34_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8793570.1"
                     /translation="ESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITA
                     DDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKE
                     KVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          182 a           67 c          105 g           97 t
ORIGIN      
        1 ttgaaagtgg aacaacagtc tttgcagtga aagacggcgt aagcttacca gaagataaac
       61 ttccagtgtt gaaagcaaaa gatggttata cagatgcgaa atggccagag gaagcaactc
      121 aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg gatgataaat
      181 ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg aataaggaac
      241 cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact aagtatactt
      301 ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt gtagtgacat
      361 atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac aatcgttctg
      421 acgccgataa atatgagcca acagtagaag g
//
LOCUS       DAYYFU010000223          418 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000223 DAYYFU010000000
VERSION     DAYYFU010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.240"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACHA34_003034"
     CDS             complement(<1..132)
                     /locus_tag="ACHA34_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8793571.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          142 a           82 c           81 g          113 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactct
//
LOCUS       DAYYFU010000224          415 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000224 DAYYFU010000000
VERSION     DAYYFU010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.241"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..339)
                     /locus_tag="ACHA34_003035"
     CDS             complement(<1..339)
                     /locus_tag="ACHA34_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8793572.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAYYFU010000225          329 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000225 DAYYFU010000000
VERSION     DAYYFU010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.243"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>329
                     /locus_tag="ACHA34_003036"
     CDS             <1..>329
                     /locus_tag="ACHA34_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM8793573.1"
                     /translation="YVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIVFT
                     MLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIWGI
                     ISKGWYM"
BASE COUNT           91 a           54 c           72 g          112 t
ORIGIN      
        1 gatatgttgg gtcgacgatt aatcccttca acgttttaat cgcacaggga gtagctggcg
       61 tttacggaaa tcccctgctt tggtttcgga tgctatcctg gattgtcatt actgggattt
      121 caattgtttt caccatgctt tatgctagca gagtcaaaaa gaatccactg tcctctattg
      181 tttatgagag tgatttaaag gttaagcata agtttcaaaa ttttgacact gaaaaagagt
      241 tttcaacaca agataaacta attatcgccg gttttatcgt aggtttagga atcatgattt
      301 ggggtatcat ttccaaggga tggtatatg
//
LOCUS       DAYYFU010000226          328 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000226 DAYYFU010000000
VERSION     DAYYFU010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.244"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..>328
                     /locus_tag="ACHA34_003037"
     CDS             52..>328
                     /locus_tag="ACHA34_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM8793574.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCK"
BASE COUNT          104 a           60 c           63 g          101 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagt
//
LOCUS       DAYYFU010000227          323 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000227 DAYYFU010000000
VERSION     DAYYFU010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.245"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHA34_003038"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHA34_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFM8793575.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DAYYFU010000228          321 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000228 DAYYFU010000000
VERSION     DAYYFU010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.246"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACHA34_003039"
     CDS             complement(<1..>321)
                     /locus_tag="ACHA34_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8793576.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAYYFU010000229          308 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000229 DAYYFU010000000
VERSION     DAYYFU010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.247"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..>308)
                     /locus_tag="ACHA34_003040"
     CDS             complement(76..>308)
                     /locus_tag="ACHA34_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8793577.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           94 a           46 c           56 g          112 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac tgaccttctg
       61 tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa tcctgatttt
      121 catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga tatggggaat
      181 cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa taccttcttc
      241 atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt caggaagatt
      301 ttttaata
//
LOCUS       DAYYFU010000230          305 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000230 DAYYFU010000000
VERSION     DAYYFU010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.248"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>305
                     /locus_tag="ACHA34_003041"
     CDS             <1..>305
                     /locus_tag="ACHA34_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFM8793578.1"
                     /translation="YTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIKIIRYF
                     FIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMI"
BASE COUNT          103 a           57 c           48 g           97 t
ORIGIN      
        1 gctatactag ccaaagagcc acgtatagag cggtttgttc cgtcttattt cctaatggtg
       61 actttcctag taggagtcga ttcactcgct taagttcaaa cttagcttac actatcaaga
      121 ttattcgata ctttttcatc aagaagctca ccaaaggtga actagtcgga attatagata
      181 gttttcctag tccattatgt aaaccagtta gaaatagaca agcaaaacta ttgaatcaaa
      241 tagccaaagt tggctataac tcaacaaaaa aatcttattt ttatggtctt aaaattcata
      301 tgatc
//
LOCUS       DAYYFU010000231          300 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000231 DAYYFU010000000
VERSION     DAYYFU010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.249"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>300
                     /locus_tag="ACHA34_003042"
     CDS             <1..>300
                     /locus_tag="ACHA34_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8793579.1"
                     /translation="DTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRR"
BASE COUNT           94 a           62 c           50 g           94 t
ORIGIN      
        1 tgatacgatc gttggcattc atcataaaag tgccgtcatt actttagttg aaagattatc
       61 taaagtcatt atcacgatta aacccaacgg ccgtaaggca ttagatattg aaactgccct
      121 taatcaatgg ttttctcgct tccctaaaaa cttctttaaa tctattacgt ttgactgtgg
      181 aaaagaattt tctaactgga aagccattag taaccaacat gatattgata tatattttgc
      241 ggaccctgga acaccttctc aacgcccatt aaacgagaat tctaacggga ttctgcgtcg
//
LOCUS       DAYYFU010000232          299 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000232 DAYYFU010000000
VERSION     DAYYFU010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.250"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>299
                     /locus_tag="ACHA34_003043"
     CDS             <1..>299
                     /locus_tag="ACHA34_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8793580.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT          102 a           63 c           54 g           80 t
ORIGIN      
        1 acgattgtcg gcatccacca taaaagtgcc gtcatcacac tcgttgagcg actttcaaaa
       61 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
      121 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      181 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      241 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DAYYFU010000233          299 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000233 DAYYFU010000000
VERSION     DAYYFU010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.251"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..299
                     /locus_tag="ACHA34_003044"
                     /pseudo
     CDS             1..299
                     /locus_tag="ACHA34_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          119 a           59 c           56 g           65 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcag
//
LOCUS       DAYYFU010000234          282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000234 DAYYFU010000000
VERSION     DAYYFU010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.252"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>282
                     /locus_tag="ACHA34_003045"
     CDS             <1..>282
                     /locus_tag="ACHA34_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFM8793581.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DAYYFU010000235          270 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000235 DAYYFU010000000
VERSION     DAYYFU010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.denovo.253"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>270
                     /locus_tag="ACHA34_003046"
     CDS             <1..>270
                     /locus_tag="ACHA34_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8793582.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGW"
BASE COUNT           83 a           56 c           53 g           78 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg
//
LOCUS       DAYYFU010000236        22234 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.guided.040, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000236 DAYYFU010000000
VERSION     DAYYFU010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.guided.040"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            218..532
                     /locus_tag="ACHA34_003047"
     CDS             218..532
                     /locus_tag="ACHA34_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8793583.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            553..930
                     /locus_tag="ACHA34_003048"
     CDS             553..930
                     /locus_tag="ACHA34_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8793584.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            940..1713
                     /locus_tag="ACHA34_003049"
     CDS             940..1713
                     /locus_tag="ACHA34_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793585.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            1735..3138
                     /locus_tag="ACHA34_003050"
     CDS             1735..3138
                     /locus_tag="ACHA34_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8793586.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3320..4504
                     /gene="mobT"
                     /locus_tag="ACHA34_003051"
     CDS             3320..4504
                     /gene="mobT"
                     /locus_tag="ACHA34_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8793587.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            4501..4791
                     /locus_tag="ACHA34_003052"
     CDS             4501..4791
                     /locus_tag="ACHA34_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793588.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            4788..5009
                     /locus_tag="ACHA34_003053"
     CDS             4788..5009
                     /locus_tag="ACHA34_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793589.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5051..5830
                     /locus_tag="ACHA34_003054"
     CDS             5051..5830
                     /locus_tag="ACHA34_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFM8793590.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            5891..6391
                     /locus_tag="ACHA34_003055"
     CDS             5891..6391
                     /locus_tag="ACHA34_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8793591.1"
                     /translation="MEMKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEE
                     YAIHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENK
                     EDIHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHG
                     VFEYCQ"
     gene            6447..7085
                     /locus_tag="ACHA34_003056"
     CDS             6447..7085
                     /locus_tag="ACHA34_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFM8793592.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7157..7552
                     /locus_tag="ACHA34_003057"
     CDS             7157..7552
                     /locus_tag="ACHA34_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8793593.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            7536..9989
                     /locus_tag="ACHA34_003058"
     CDS             7536..9989
                     /locus_tag="ACHA34_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8793594.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            9986..12013
                     /locus_tag="ACHA34_003059"
     CDS             9986..12013
                     /locus_tag="ACHA34_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8793595.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12010..13032
                     /locus_tag="ACHA34_003060"
     CDS             12010..13032
                     /locus_tag="ACHA34_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFM8793596.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13049..13978
                     /locus_tag="ACHA34_003061"
     CDS             13049..13978
                     /locus_tag="ACHA34_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8793597.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14292..14339
                     /locus_tag="ACHA34_003062"
                     /pseudo
     CDS             14292..14339
                     /locus_tag="ACHA34_003062"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14355..15155
                     /locus_tag="ACHA34_003063"
                     /pseudo
     CDS             14355..15155
                     /locus_tag="ACHA34_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            15393..15629
                     /locus_tag="ACHA34_003064"
     CDS             15393..15629
                     /locus_tag="ACHA34_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793598.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            15636..17588
                     /locus_tag="ACHA34_003065"
     CDS             15636..17588
                     /locus_tag="ACHA34_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8793599.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(17660..18157)
                     /gene="dfrG"
                     /locus_tag="ACHA34_003066"
     CDS             complement(17660..18157)
                     /gene="dfrG"
                     /locus_tag="ACHA34_003066"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8793600.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            18432..19559
                     /locus_tag="ACHA34_003067"
                     /pseudo
     CDS             18432..19559
                     /locus_tag="ACHA34_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            19657..19845
                     /locus_tag="ACHA34_003068"
     CDS             19657..19845
                     /locus_tag="ACHA34_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFM8793601.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(19903..20256)
                     /locus_tag="ACHA34_003069"
     CDS             complement(19903..20256)
                     /locus_tag="ACHA34_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8793602.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            20787..21209
                     /locus_tag="ACHA34_003070"
     CDS             20787..21209
                     /locus_tag="ACHA34_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8793603.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            21206..21436
                     /locus_tag="ACHA34_003071"
     CDS             21206..21436
                     /locus_tag="ACHA34_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8793604.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            21934..22134
                     /locus_tag="ACHA34_003072"
     CDS             21934..22134
                     /locus_tag="ACHA34_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFM8793605.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
BASE COUNT         7573 a         3696 c         4297 g         6668 t
ORIGIN      
        1 tttgattata atatttaaaa tacctaggta gtcgttgttt taaatatagt aggaatcgga
       61 cgctcagtac ggagcgtcta tttttttacc caattttgaa aggaagtgaa cttatgaaaa
      121 taaaaaatca agaatcaaaa ggtcgctctc ccccctttaa aaccatcaca caaataagat
      181 tcattcaaac ataaaaacag aaaggatagg taaaaatatg gaacttaaat atgtcattcc
      241 caatatggaa aaaacatttg gacaattaga atatgctggt gaagggaaca ttgaacaacg
      301 tcgaataaat ggcagaccaa ccattctctc tcgtagttac aatttatact caacgattca
      361 acgagcagat gatattgttg tcattcttcc ctccgaagct ggcgagaaac attttgaatc
      421 cgacgaaaaa gtcaaactca tcaacccaca tattaccgca gaaggttata aaattggcaa
      481 tcgaggattt accaattaca ttatgcacgc agacgatatg gtgaaagcat aagaaaggaa
      541 aggtgaaaaa atatgagatt agcagaaggt attgtgattg aaaaagacca gacatttggc
      601 acattgaaat tttccgcctt gcgtcgtgag gttcgtatcc agaatgaaga tggtacggtt
      661 tccgaagaaa ttaaagaacg tacctatgac ttaaaatcaa aaggacaagg acgcatgata
      721 caagtcagta tccccgcttc tgttcctttg aaagaatttg actacaacgc agaagtggaa
      781 ttaatcaatc ctgtggcaga taccgtagcg acggctactt ttcgaggggc agacgttgat
      841 tggtacgtga aagcagacga tcttgtatta aaaggacaga caaaacctca tcagaaatat
      901 aaaaaagaag cgtccgaaag tgaaaaatag gtgttgctta tgacaaacac accattgatt
      961 ttgaaagaaa ttccgaagga tgaagccatt tcttttattc ggcaatacca ttattcaaag
     1021 atactcccta gattgtgtaa atactttcta gggatttttt cagaagaaaa attattaggc
     1081 gttgtggaat taggctgggg tacgcaaccc ctacaaacca tacgcaaact ctttcccgac
     1141 agttcccttc aaaccacaga ttatttagaa attggaaaga tgtgttttct tccagaaatg
     1201 aatcaaacca attattttgg cagtcaagct ctttcagctc taattaagtg gttgaaagaa
     1261 catacggact gtcatttttt atatacactg gcagatggaa ttgagggaaa atgtggctat
     1321 gtctatcaag cgtccaactt cttttactgc gggtatttca agacaagtgt ctatcgtgat
     1381 aagcagtcat gggaaaagat acaccctcga agtgctcgcc ttttattaga ggaaaatgct
     1441 cgctttgaac aggtagagaa aaagcattgg ctttcccagg cattttgcga atacaaaggg
     1501 attgagaaaa tcaatggacg catgtttcgc tatctctatc cgttgacaaa agaagcgaaa
     1561 aaactgttag gacacactct ctatcgacgt cattattatc cgaaagaaaa aaatttacgc
     1621 tttgaaaagc gaatcgctta tcagaaatat gaagccattt ctcaaccaac ttttgataaa
     1681 caggctcgta tctacaatac acaattattt tgataggtgg aaaggaggat ttttatgcca
     1741 ttgcttcatg acagaggaga gcgaatcaaa gccaacgata agcacttggt ctatcatttt
     1801 gtactaattt cattgctggt agtttttcta gtcgtcctac tcccttttca ttggagagcg
     1861 ttacaatcgg tgaactggca agcagttcag ttagatacat ttttctatca acttcatacg
     1921 ccatactttt ggagcagtat tctcactgct ctttttgtat gcggacttat cagctggctt
     1981 tattatcact atcgtataga ttctataaaa cagttagagc accgtcaaaa gttagctcgt
     2041 atgattttag aaaatcattg gtatgaagcc gaagaaactc aatcagaggg attctttaaa
     2101 gatttaccca gcaagaaaac caaacaacga atccgccatt ttcctaaaat atattatcga
     2161 atgaaagatg gactaatcca tattcatgta gaaatcacct tagggaaata tcaagatcag
     2221 cttttgcacc tagaaaacaa actagaaaca ggattgtatt gtgaattagt ggaaaaaacg
     2281 ttgaaagatt cttatgtaga gtatgtccta ctttatgata ctattggcaa tcgaattggc
     2341 attgaagacg tggtagctca agatggacgt gtcaaattga tggaatcggt ctattgggag
     2401 ttcgattctc tccctcacat gttaattgct gggggcactg gtagtggaaa atcctatttt
     2461 attctcacat tgattgaatc tctcctgcat acaaatgcaa agctttatat tcttgaccct
     2521 aaaaatgctg atttggcaga cttaggggcg gtcatgaata atgtctatta ccgtaaagaa
     2581 gatatgttgg catgtatcaa tcaattttac gaagatatga ttgatcgcag tgaaaccatg
     2641 aaacaacacc ctaactataa aacaggagaa aattatgctt atcttggttt gtctgccaat
     2701 tttcttattt ttgatgaata tgtggcaatg atggatatgc taggtcgtga aagtaccacc
     2761 gttattcata aactcaaaca aattgtaatg ttaggacgac aagcaggctt ctttttaatt
     2821 cttgcctgtc aacgtccaga cgcaaaatat tttagtgatg ggattcggga caactttaac
     2881 ttccgtgtgg ctttgggtcg tatgtctgaa atgggctttg gtatgatgtt cggaagcgat
     2941 acacaaaaac aatttttctt aaaacccatc aagggaagag gttacgtgga tacaggaaaa
     3001 agtgtgatta gtgagtttta tacacccctt gtaccaaaaa ggtacgattt cttaggagaa
     3061 ataggaaaag tcattcaaaa aaagcaatct gagcctgtac cgcacgaagt gaaaggtaca
     3121 ggttcagact tgctttgatt ggtgtggcgt tagccacgcc aatcctttag ccccctctat
     3181 ctaacagggg ggcacaaata caactgaaaa caggcaattc tcatcaacca atgttgatgg
     3241 tacaaaggat tgcggaaaat atgaaaggtg taaactcaag ggtcatagtt tacatctttt
     3301 ttagattgga ggtttgcgaa tggaaaacga aaaaatttgg attaaggaat tgaaagaaaa
     3361 gcgactggct tatggcgttt ctcaaaataa actggcggtt gcttctcata ttaccagacc
     3421 ttacctatcg gatattgaaa caggcaaggc agtccctact caacaggtga aagaagattt
     3481 actgaacgct ttggaacgct tcaatcccga taacccattg gagatgttat tcgactatgt
     3541 acggattcga ttccctacca atgatgtggc tcaaattatc ggtgaggttt tacgtttgaa
     3601 tatggattat atgctacacg aagactttgg ctattattct tatcccgaac actatcgttt
     3661 cggtgatatt gttgtcctag tctctcatga tgtaagtaaa ggtgttttat tagaactgaa
     3721 aggaaaaggc tgtcggcaat ttgaaaactt tctattggct caacatcgga gctggtatga
     3781 tttctttcaa gattgcatgg aacataaagg tattttcaaa cgcttggatt tagctatcaa
     3841 cgataaaaca gggattttga atattccaga tttggcaaag aaatgtaaac aggaagaatg
     3901 catttccgtc tttcgaagct ttaaaaatta tcgcagtggt gaattggttc accgtgatga
     3961 aaaaccatac atgggaaaca cactttatat tggctcatta aaaagtgaag tctatttctg
     4021 tatatatgag aaagattatg agcaatacgt gaaaaatgat gttcccttag aagacgcaga
     4081 agtaaagaat cggtttgaaa ttcggttaaa aaatgaccga gccacccaag ccatgaaaga
     4141 tttattggca catcaacagg cggaaaaaac agcctttgaa attatcaatc gctatatccg
     4201 ctttgcagat aaagacgaca ctaaacgtcg gagtgattgg aaaactaatg aacgttggga
     4261 atggtttatc ggtaaaaatc gtggagcatt acgcctaacc acacagccag agccttattc
     4321 ttttgaacga acattgaact ggttacatca tcaagtcgcc cctacattaa aaatcgcaag
     4381 cattttagat gttctcaacg gtacaaccat tatctccacc atgattcagg aagccaaact
     4441 gacagaaaaa catgagaaat taattgaaca acaacatcta gccatggagg atttgattac
     4501 atgagccttt taacaaaaga attaaagaag ttagggtttc agtgtggcat tgagtttcaa
     4561 gcgtatatac aaaatacagg gaaatatacg tctctcatta ttgagggaaa acggcaagct
     4621 ggtgatacca tctataccta tgatttttac aaggtgagat tttataataa ttataccaat
     4681 cgagtaaccg tctatggaga gcatttaacc ccttttcaat tattaaggcg tgtaaaaagc
     4741 tatatctatt atcgagaaaa atatcttaag gaaaggagga caatcacatg aattttgggc
     4801 agaatttgta caactggttc ttggataatg cacagtcatt ggtattgtta gcgattgttg
     4861 tcattggact gtttttaggt tttaaacgag aattttcaaa actcattggc ttcttgatta
     4921 ttgctctaat cgccgtcgga cttgtgttca atgcagctgg cgtgaaagat gtgttattaa
     4981 acttatttaa ccgaatcatt ggtgcttaac agatattttt catgataaaa taggaagtat
     5041 atattttgtt atgaaaggat ttatgaatat gtttacaaat gatcaattta aatctctttt
     5101 aattaaaaca ttagaaagaa agagctcttc aaattattat gaaggaggta acgagattgt
     5161 ttctaaaatg aaaatatcag aggtaacttt agatgagact gacgatttta cttattataa
     5221 aggatataaa aagggctgga atacgctttg cacatatctt aaaataagag ttccttttga
     5281 tgatttagac ttttttgaaa gtcataaaga tttgattacc cagacagcaa gctccatata
     5341 tgataaacaa ggtgataatg tattagtgga tactatttta gtaccacttc cagagaatta
     5401 cgaagtaatt aactttagtc agttgaaaat tagtgatgtg gtttcacaag ctattgaaga
     5461 cgctgagtct tttatgagta atggtgaata tcaacgtgcc tttgacagag ttcacactgc
     5521 ttttcatgga tatttaattg aaatactaaa aaaatatgaa attacagttc ctagagacga
     5581 aaatctatct aaattatata gtaggataca acaattaatt gaaaaagaaa ttcaacctac
     5641 tgaacttgct gatatagtga agacaacgat aaggtcatca aatggaatga ttagctctct
     5701 taatgaagca agaaataggc attcacttgc acaccctaat acaaatatta ttggaaagag
     5761 agaagcaaaa ctaataatag gtatttcaag tacagttaca gattatatta gcggatacct
     5821 tgataagtag cgtcattaac tgattgctac tttttttatg actattttta taaaaaggat
     5881 gtgataagaa ttggaaatga aagtctatgt ggcaaattta ggacggtata atgaaggtga
     5941 attagtcgga gcatggttta ctccaccaat tgatgaggaa gaaatggcag aaagaatcgg
     6001 tttaaatgag gactacgaag aatatgcaat acatgatttt gagcttcctt ttgatgtgga
     6061 cgaatacacg ccgattagtg aaatcaaccg tctatgtgaa gccattcaag aaatcgaagg
     6121 tacacctatt tataatgaac tcaaagaaat tcaaggcatg tggtttagta gcttggaaga
     6181 attactggaa aacaaagagg atattcattg ttatagtgat tgtgattcga tggaagatgt
     6241 cgcccgttat tacgtggaag aaacaggaca gcttggtgaa gtcccttcca atttacaaaa
     6301 ttatattgac taccaatctt tgggacgtga tatggaaata gagggtaatt acctagttac
     6361 ctctcatggg gtatttgaat attgtcagta agtttaacaa tccgtgttat aattaaatta
     6421 ataaatagca cggagaggta taaattatgg taaatgtatt tgaaaatctt aaactattaa
     6481 aactagacat gaaagataaa ggctgggtga tagattcctt ttactttcgt tacaaacagc
     6541 aaaattatat tgtacttgtt aagctatttg aaaaagagga aaaagttcca gaatatgctc
     6601 tgttaaaact agaattttta aaggaaaacg atttttcaga tatgctagct gtctatgcta
     6661 attcggtaaa attatataca gacacgaaaa caataagaga atattttggt atcgaataca
     6721 gtagtaactt gggagatgtt ttatttcaat ttagccaaac tcttgctcga tttattccga
     6781 cagaagttag tgagaagaaa aatgaagacc aaaaagaagc catgtgtttc tcattaagtc
     6841 aaagtgattc agaagatccc agaaaaaaat attgtttttc agttagaaga aatcctttaa
     6901 agggaaatgg tgagctggga aaacgaagtc cttttaatga caataaaacg agattgtata
     6961 gacccagctt atatgaacga ttagggtcag ataccaattt aagtttcaga tattcaatga
     7021 atccagatga tgaagaaact gatgaaggta ttattgcaaa atggacaaaa aataaaattg
     7081 aatagttaaa gcattgaaag agcaatcacc tattttgatt gttctttttc tatttttacg
     7141 gaaaggacgt gaagacatga aaaaaataag aagttacacc agtatttggt cagtagaaaa
     7201 agtgatttat gcgattaatg atttacaact ccctttccca attaccttta cgcaaatgac
     7261 atggtttatc gtttctttgt ttgtcgtcat actctttggc agtttaccac cactttctct
     7321 gattgacgga gcactactca aatacttagg gattcccatt gttctcactg ggttcatgtg
     7381 ccaaaagacc tttgacggaa agaaacccta tggatttcta aagtctatca ttacctatgc
     7441 tctacgccct aaagtaacct acgctggcaa acaaatacga ttaaaaaaag aaacaatgaa
     7501 tgaatcgatt acgattgtaa ggagtgaaca actagatggc atatccgatt aaatatatgg
     7561 aagataatct cgtatttaat catgatggcg aagtattcgc ttattatgaa ttaattccct
     7621 ataattacag ttttttaagt tccgatgaaa aaattcaagt tcatgataat ttctgtcaat
     7681 tgattgccca aaatcgtgat ggcaagattc atgctctgca actagcaacc gaatcaagta
     7741 ttcgagccac acaggaacaa tcgaaaaaag aaatcactgg acgattaaaa gagatagctt
     7801 acgaaaaaat agatgaacag accgaagcgt taatttctat gattggtgac aatcaagtag
     7861 actatcgctt ttttattggt tttaaactct acctcaatga acaggaagta tccgtaaaaa
     7921 atgttgggaa agaaattcaa aaagcatttg cggaattctt tcatggtgtg aatcacaaat
     7981 taatgggcga ttttgtttct atgcctatgg cggaaatcga acgcttttct aagatggaac
     8041 agttgttaga aagtaaatta tccagacgct ttcagattcg taaattggag aaagatgatt
     8101 ttggttatat tattgaacat ctttacgggc aaacaggtgt cccttttgat gactacgact
     8161 accatttacc gaaacgatac gaagataaaa aagtgttggt aaagaaatac gatattctca
     8221 aacccactcg ctgtttaatc gaagaaaatc aacgctattt acggattgaa caggaagata
     8281 ccactactta tgtggcgtat tttacaatta atagtattgt tggtgaattg gatttcccta
     8341 atagtgaaat tttttattac cagcaacagc agtttacttt ccccgttgat accagtatga
     8401 atgttgaaat tgtcgccaat aaaaaagcct tatctaccgt tcgcaataaa aagaaagaac
     8461 tgaaagattt agatagccac gcatgggaaa gcgacaacga aaccagtact aatgtggtgg
     8521 acgctttaga atctgtcgat gaattagaaa gtaatttaga ccaaaccaaa gaatctatgt
     8581 acaaactaag ctatgtggta cgggtatcag caaagaattt ggacgagctg aaacaacgtt
     8641 gtgatgaagt aaaagatttt tatgatgatc tgtctatcaa attagtacga ccttttggcg
     8701 atatgctagg cttgcataat gaatttattc cctcaagcaa gcgatatatc aatgactata
     8761 ttcaatatgt cacgtcggat tttttggctg gtcttggttt cggtgctaca caaatgattg
     8821 gtgaaaattc tggtatttac gtcggttaca atctcgatac aggcagaaat atttatctcc
     8881 aacctagcct tgcaagtcaa ggaattaaag gctctgtaac aaatgcatta gcgtccgctt
     8941 ttattggttc attaggtggc ggaaaatcat tctctaacaa tatgctggtc tattattcag
     9001 tactctttgg cggacaggca accattgttg acccaaaagc tgaacgtggt gaatggaaag
     9061 aaaccttacc tgatattgcc catgaaatca atattgttaa tctcacgagt gagaacgaca
     9121 ataaagggtt acttgaccct tatgtgattt taaaactccc gaaagattca gaaagccttg
     9181 ccattgatat tctcacgttc ttaacaggta tttccagccg agatagcgag aagtttcctg
     9241 tcctgcgtaa agctattcgg agtgtaactc aaagcaaaca acgagggttg ttattggtga
     9301 ttgatgaatt gcgaaaagaa gatacgccca tcagcaatag tattgctgac catatcgaaa
     9361 gctttgtcga ttatgatttt gcccatctgt tattcagtga tggcagtgtc acccaatcca
     9421 ttagcttgga aaaacaactc aatattattc aagtggcaga tttggtctta cccgattcca
     9481 atagctcttt tgaagaatac actaccatgg aattattaag tgtggcaatg ctgattgtca
     9541 ttagtacctt tgctttagac tttatccact ctgaccgttc catttttaaa attgttgacc
     9601 ttgatgaggc ttggagtttt ttacaagtgg cacaaggaaa agctctatct atgcgattgg
     9661 ttcgagcagg acgtgccatg aatgcagggg tctatttcgt tactcaaaac gcggacgatt
     9721 tactagatga aaagatgaag aataatattg gcttgaaatt tgcctttcgt tccactgacc
     9781 tagtggaaat caaaaagacc ttagaatttt ttggtgtgga caaggaagat gaaaacaatc
     9841 agaaacgctt acgtgaatta gaaaatggac aatgtttagt ttcagacatc tatggtcgtg
     9901 taggggttat gcagtttcac ccgatttttg aagagttgct tcatgccttt gataccagac
     9961 cgcctgtgcg aaatgaggtg gaagaatgac acaaaaacaa aagaaatttc ttcactatag
    10021 ttggataacg ctcctttgtg tgggcgttat tttactttta ttaggcacgc tgggcaatgc
    10081 agtacaagcg acaggcttag ttgatgaaac cattgataca agcaatgaat actccaagta
    10141 tggtttgaat caatatcagc ttgattatta tgtggacaat agctggggct ggcttccatg
    10201 gaattggagt gatggcatag gacaatcggt gatgtatgga ttgtatgcca ttactaattt
    10261 tatttggaca atttctcttt atctatcgaa cgccacaggt tatttggtga aagaagccta
    10321 tgccttagat ttcattagtc aaacatccca agcaattggc gaaaacatgc agacgattgc
    10381 aggcattaca ccgaatggct tttccagttc agggttttat gtcggtttcc tccttctttt
    10441 tattctcatt gttggaatct atgttgccta tacaggattg gttaagcgag aaacgacgaa
    10501 agtgatacga gctgttttaa actttgtcgt ggtgtttatt ctatcggctt cctttattgc
    10561 gtatgcaccc gattatattg cgaaaatcaa tgatttttcc aaagatgtca gtaccgccag
    10621 tctgaatatt gggacaaaaa ttgttcttcc taacacggat agtcaaggga aagacagtgt
    10681 ggacatgata cgtgacagct tattctctgt tcaagtaaag caaccatggc tattgcttca
    10741 atatggctcc actgatgtag aagcgttagg ggaagaacgt gtggaaagtt tattgtctac
    10801 cagtccagat acaaacaatg gtgaagatag agaaaatgta gtgattgaag aaattgaaga
    10861 tcatgacaat acctatttaa ccctaccgaa gacgatttcc agattaggaa cagtcttctt
    10921 tttgtttgta ttcaatattg gtatctcgat ttttgttttc cttctaacag gaatcatgat
    10981 tttttcgcaa gtgctattta ttattttcgc tatgtttttg ccaatcagtt tcctattaag
    11041 tatgttgccg agttttgatg gcatgggcag gcatgccatt accaagttat ttaatgtgat
    11101 tatgacaagg gctggtatta ccttgattat taccgttgcc tttagtattt ccactatgct
    11161 ttattcgctg tcagcaagtt ctcccttttt catgattatg tttttacaaa ttgtgacctt
    11221 tgcagggatt tattttaaac tgggcgattt aatgagccta ttcactttac aaagcaacga
    11281 ctctcaaaac atgggcagac aagtgtttcg cagacctcgt caaatgatga atcgacaaac
    11341 cagaagattt caacgcagta tgagtaagat ttttcgtgga aaagcagggt caacgaagca
    11401 aaagacaatg acaacaaagc agaatcaaac caataaagct aaccatactc gtcaaaatga
    11461 acggactaca ccaaagaaag aaacagcact ttccaatgtg aaaaagcaac aaggcggtat
    11521 cgggcaacga ttaggtgaaa agacagccca tgtattggac acgaaaaatc gtctagtcaa
    11581 taaaagaaag caggcaaaac aacagataca ggcgacacca acccatgtca aatatgccct
    11641 tcatcaagga aaagagaatg tcactcataa tgtaacagac tttaaacaat ctatggcaga
    11701 taccaaagct caacgcaaac aacagcgtat gaaccagcag gagcaaaaac gaaagaacat
    11761 tgctcaaaag cgtttggagc tggataaagc aaaagaaaaa agacgaaaag aaaacccatc
    11821 atctcctgtt cagaataacc agcagcatgt tcgcaagcgt cagcaaaccg tcacgcctgt
    11881 tttgtcacaa aacactaaac gtgaaaatca tcagcgacca ttgaaaaaag agcaaaaaca
    11941 aaagacacca ccgaagcaat tcaatcagag gaagctaaat aaaagaacgg ataagcgagg
    12001 acaacaaaca tgaagctaaa aataacagtg atttgtagtg tggtcttctc tctcttcttt
    12061 tttctcttat tattcctagt cattttcttt gcagatgata cggacagtgg cgaaaacaac
    12121 aaggattctt ctattccaca aggcggtgtg accgtttcac cagaagtgtt ggcacaccgt
    12181 ccgttgattg aaaaatatgg taaggaatat gggattgaag actatgtttc ttatatctta
    12241 gccattatgc aagtggaatc gggcggtaca gcagaagatg ttatgcaaag ttccgaatct
    12301 ttgggtttac cgcccaacag tttgagtacc gaagagtcca tcaaacaagg ggttaaatat
    12361 ttcagtgaat tattaaccag tgcagaacaa caaggagtgg atatagattc tgttatccaa
    12421 tcttataatt atggcggtgg ctttttaaat tatgtgagga gtcatgggaa aaaatatacc
    12481 tatgaattag ccgaacagtt ttctaaagag aagtcgggtg gtcaaaaagc agactatccc
    12541 aatcctatag ccatacctgt gaatggtggt tggcgatata actatggaaa tcagttttat
    12601 gttcagttgg tttcacaata cctaaccgac acttccccaa cagaatttga tgatgaaacc
    12661 gtgcaagtca ttatggacga agccttgaaa tacgaagggt ttccctatgt ctttggaggg
    12721 gcgtcgccta ccacctcttt tgattgtagt ggcttaatac aatgggtgta tgacaaagct
    12781 ggcatttctc tccctcgtgt cgcccaagat cagtatgacg ctacgcaaga aatctctatg
    12841 gaagaagccc aagcaggcga ccttatcttc tttcattcaa cctataatgc aggcacatat
    12901 gtgactcatg tggcgatata tttagaggga aatcgctttt atcatgcagg cgacccaatc
    12961 ggttatggcg acttaagcag tcgttattgg caagaccatc tgattggggc tagacgtgtt
    13021 attcataact aagaaaggaa tgaatcaaat gattataaaa atcgagcgaa aagaaaagaa
    13081 ggagaaacca aagaaatcta aaaaaccgag caagcaaaga aaagttccga tgattaaagt
    13141 tggcacgcat aagaaattaa cctttgtttt atgggtttta ttgattggta gtgtcggatt
    13201 tggaatctat aaaaatttta ctgccattga cactcatact gtccgtgaaa cagaaatcat
    13261 taaacagcag attgtcgata ccaaccaagt agaaagtttt gtgaaatcct ttgcgaaaga
    13321 ctacttttcg tggcaacaat cccaagaagc cattgataaa cgaaatgaaa aattgaccca
    13381 ttatttgacg gaagaattgc aggtattaaa tgaagaaatg attcgtaagg atattcctac
    13441 tagttctagt gtgaacgata tacaggtgtg gcaggtttct caagtgaatg agaacacctt
    13501 tgaggtactg ttttctgttg aacaagttat taccgaagac aaagacaagg aaactatatc
    13561 gtctagcttt catgtggttg tccatataga tgaatcagat aatatggtta ttatcaaaaa
    13621 tccgacaatg agtaagaaac ctcaaaaatc ggactaccaa cccaaacagc tagaaagtga
    13681 ccatacagta gatacagaaa cgatggacga aatcactagc tttttagaaa cgttcttcca
    13741 gctctatcct accgccacag aaaaagaatt gacttactat gtaagtaatc atgtgttacc
    13801 tatgattaac aaggaatatg ttttcgagga attggtgaat cctatcttta ccagaaaaga
    13861 taaccaagta atcgtgaatg tcgctgtgaa atatttagat caagaaacaa aggcaaccca
    13921 aatctcgcaa tttgagttga tattagaaaa acaagataat tggaagattg taaaataaca
    13981 aatattggta catgattaca gatactttgt aatcatgtac tctttttgat aaaaaattgg
    14041 agattccttt acaaatatgc tcttacgtgc tattatttaa gtgactattt aaaaggagtt
    14101 aataaatatg cggcaaggta ttcttaaata aactgtcaat ttgatagcgg gaacaaataa
    14161 ttagatgtcc ttttttagga gggcttagtt ttttgtaccc agtttaagaa tacctttatc
    14221 atgtgattct aaagtatcca gagaatatct gtatgctttg tatacctatg gttatgcata
    14281 aaaatcccag tgataaaagt atttatcact gggattttta tgcccttttg ggtttttgaa
    14341 tggaggaaaa tcacatgaaa attattaata ttggagtttt agctcatgtt gatgcaggaa
    14401 aaactacctt aacagaaagc ttattatata acagtggagc gattacagaa ttaggaagcg
    14461 tggacaaagg tacaacgagg acggataata cgcttttaga acgtcagaga ggaattacaa
    14521 ttcagacagg aataacctct tttcagtggg aaaatacgaa ggtgaacatc atagacacgc
    14581 caggacatat ggatttctta gcagaagtat atcgttcatt atcagtttta gatggggcaa
    14641 ttctactgat ttctgcaaaa gatggcgtac aagcacaaac tcgtatatta tttcatgcac
    14701 ttaggaaaat ggggattccc acaatctttt ttatcaataa gattgaccaa aatggaattg
    14761 atttatcaac ggtttatcag gatattaaag agaaactttc tgccgaaatt gtaatcaaac
    14821 agaaggtaga actgtatcct aatatgtgtg tgacgaactt taccgaatct gaacaatggg
    14881 atacggtaat agagggaaac gatgaccttt tagagaaata tatgtccggt aaatcattag
    14941 aagcattgga actcgaacaa gaggaaagca taagatttca gaattgttct ctgttccctc
    15001 tttatcatgg aagtgcaaaa agtaatatag ggattgataa ccttatagaa gttattacta
    15061 ataaatttta ttcatcaaca catcgaggtc cgtctgaact ttgcggaaat gttttcaaaa
    15121 ttgaatatac aaaaaaaaga caacgtcttg catattaagt agttcagttt tggagtacaa
    15181 aaagtgaaaa tgtaaaccta aatgagtatc aaattgcaac tgaatttgat actcgttttt
    15241 tattgttaaa tcaacgtttg ttgttaattt acaccacaaa atgtaagtta aaatgtatat
    15301 aaaaatgcga acgtgaaata attattgtag gaatgcaaat tggtgtgtta aaatggttac
    15361 aatttactaa gcaaaaaggg gtgcattttc ctatgcaatt tcttgagcca aaaaataaga
    15421 acgctaagtc tgtggattgg gaaatatcag agcaagtaag ggtgatagtt aagcaatacg
    15481 ctgaatatgc agagcgtact gaaagcgaag cagtggatga attcttattg aacattttag
    15541 acgataagaa gtttattgaa tggattgcca acaagcgaag taataagcgg attgttgaaa
    15601 aaatgggtat caaagataga gtgggttgat agacaatgac caagcttaaa agattagcca
    15661 cgaaggaaga tgaaattgta gatgttaaaa ttccgatttc aaacgaagaa ttaaaagata
    15721 gggctaaaca atatgattta ttaactccaa aacgatttgc aacaaggtat aacaaaatgt
    15781 tgttcttgcc aacgtctttt aaatggaacg gttctgaata tcccattcaa tataattact
    15841 gcatcaatcc gttttgttgt aattttggga aagaacagca caaattcaaa gatgtaaaag
    15901 ggaaaccaag tcgatataaa atgactggta gttccaaaga taaaggtcat aaaggtatgt
    15961 actgcaatga caatcctatt ggcagaggtg tatcgcaaaa ctgtacagta acccctttat
    16021 ctaattggtc tgttgttgaa gaaattaaac gccttataga aatcaatagc atccaagatg
    16081 ttgagccaga ttatcaattt cataaagaag gctgctcgga agaagaatca accccattca
    16141 atgagccgaa gcaattttac aaaaggggga aaagtagggg caaatctcaa cgctatcaat
    16201 gtaaggcgtg taagaagttc actaatgttc taccaaaaag agaagaaacc actacttatc
    16261 accaacaaaa gaacaccatt cttccaatgt tcgctaagat ggttgttgga agggtttcag
    16321 tcagtcgtac ttgtgatatt ttaggaattg gagttggaac ctactatcat aagctggagt
    16381 ggctgtatag gcgttgtttg gagtttttag aacgatatga gacacaaccc cttcaaacaa
    16441 agaaatttaa cgagatgtgg cttaatacgg acaagatgca ctactaccta aataacgtcc
    16501 gtaagaaagg acaaggttca aagaaatata cggggtttga agaccttaat atgcaaacgt
    16561 atatagttgt atctgctgag gtcttatcaa gatatgtgtt ccgttctgat gttgcctatg
    16621 attggaatat ttcaatggat gaattaaatg aagatacaag aaaattcaaa gaagaccatc
    16681 tcaatacttt tagtcgtaaa aatgataggt tagattggtc ttattatcca caagaaccat
    16741 ccgctaacga tagcgaaaat aggaatgctt atcttcacga attagggaaa attactaata
    16801 ggagtagatt tgtcgatggt ttaaatgttg atgctcctta tactacaact gctcactatt
    16861 ggttaattaa acaaatggtt aatgccgacg aatggcgaat gattagtgat gatgacttct
    16921 ctattagaaa cgccttttat agagtattca cgaaagaatt aaggttgagc gatgcacatc
    16981 attttatttg ccaagttaac aagacgaaaa gcagaaaaca atgcttaaaa gaattcggac
    17041 aagcaaaagc agaactgctc gattggggag atatcagagg attcaaaaca aagtttctac
    17101 ggactttggc atctcattat ctaactgaat tactaacaag tcatcaattc cacgaagagg
    17161 ctattagtaa agatggagaa agatatcgca aatatgccga taaccctatc aaacatccat
    17221 tagccactaa ggataaagga ttttattcag ttgattgtag aacggattta tctgctctgg
    17281 aaccaaacga aatcgcaaag atgctattaa atgttaatga ccattcgacc aatagcttta
    17341 ttcaacagat aagacgttat atatcatctc ttgaaagacc attaacaaca gctcgtggtg
    17401 ataagaagag ctatatttac gctaacttca atccgaagta tgctcaattt gcgattacaa
    17461 ttctaagaac ttactacaac ttttgtcgcc cttttaaatc agcggataaa aaggttttga
    17521 cacctgctca aagattaggg attacagata aacaatttga ttggaaagat ataatttatt
    17581 tcaaatgaca aaaagagctt ggagtaatcc caagctcttg tgctttatct ttactgttct
    17641 tcttcaatta acgcacccgt taactcaata agttttttct ttcatataca tgaaaatagt
    17701 agttatacgg atttttatca ttctttatcc ctttttgggc aaatacctca ttccattcct
    17761 cataattcac ttctggaaaa aaagtatctc cttcgaattc atgatgtatt tttgtgatgt
    17821 acattttctc aacataaggg aaaaacaaat tataaatctg ttctcctccg aaaataaaaa
    17881 tttcttcttc gtttttacat aactcaaaaa catcttctat tgaatgaaca atttcacaac
    17941 cattaaaggt aaacccctta tctctcgtca gaataatatt tcttctgtca ggtaaggctc
    18001 ttccgattga ttcaaggttc ttcctaccta atattatcgg atgtcccttt gtagtatttt
    18061 taacatattc ccagtccttg ggaatcctcc aaggaatgtc attctctttg ccaatcactc
    18121 tattcttatc catcgcagca atcaaagaaa ctttcattta taacacagcc tttttaacta
    18181 atttaccatc taatttctta tttctatcaa aaaacctttt ctcattatcc tgcccgttag
    18241 ttgaataagc aattttttct ataagttata ttatcataat accacaaaat cacaaatttt
    18301 cattcaatta tcattttcaa ttgcatttta taaatcattt tgtatgtaat ttggtatgta
    18361 agttaaaatg aaaaaagtag tccattttga agtacatttt gtcattttgt actccaaaac
    18421 tgaactactt acttgcatat atacgccttt atagtggagt actacattta cgagattcgg
    18481 ttagagtatc agaaaaagaa aaaataaaag ttacagaaat gtatacttca ataaatggtg
    18541 aattatgtaa gattgataga gcttattctg gagaaattgt tattttgcaa aatgagtttt
    18601 tgaagttaaa tagtgttctt ggagatacaa aactattgcc acagagaaaa aagattgaaa
    18661 atccgcaccc tctactacaa acaactgttg aaccgagtaa acctgaacag agagaaatgt
    18721 tgcttgatgc ccttttggaa atctcagata gtgatccgct tctacgatat tacgtggatt
    18781 ctacgacaca tgaaattata ctttctttct tagggaaagt acaaatggaa gtgattagtg
    18841 cactgttgca agaaaagtat catgtggaga tagaactaaa agagcctaca gtcatttata
    18901 tggagagacc gttaaaaaat gcagaatata ccattcacat cgaagtgccg ccaaatcctt
    18961 tctgggcttc cattggttta tctgtatcac cgcttccgtt gggaagtgga atgcagtatg
    19021 agagctcggt ttctcttgga tacttaaatc aatcatttca aaatgcagtt atggaaggga
    19081 tacgctatgg ttgcgaacaa ggattatatg gttggaatgt gacggattgt aaaatctgtt
    19141 ttaagtacgg tttatactat agccctgtta gtactccagc agattttcgg atgcttactc
    19201 ctattgtact ggagcaagcc tttagaaaag ctggaacaga attgttagag ccatatctta
    19261 gttttaaagt ttatgcacca caggaatatc tttcacgggc atataacgat gctcccaaat
    19321 attgtgcaaa tatcgtaaat actcaactga aaaataatga ggtcattatt attggagaaa
    19381 ttcctgctcg atgtattcaa gattatcgca atgatttaac tttttttaca aatgggctta
    19441 gtgtttgttt agcagagcta aaaggatatc aggttaccac tggcgaacct gtttgccaga
    19501 cccgtcgtct aaatagtcgg atagataaag taagatatat gttcaataaa ataacttagt
    19561 gcgttttatg ttgttatata aatatggttt cttattaaat aagatgaaat attctttaat
    19621 atagatttga attaaagtgg aaaggaggag attgttatta taaactacaa gtggatattg
    19681 tgtcctattt gtggaaataa aacaagacta cgaatacgag tggatactat acttaaaaat
    19741 ttccctttat actgccccaa atgtaagaac gaaactttaa ttaatgttca aaaaatgaat
    19801 ataataacaa tcaaagagcc agacgccaag acgcagagcc gataatttga gaaatgaaac
    19861 tctcatctta tcggctcttt ttgtttatct gaattttact gactagcctt caatatttct
    19921 tgcttcattg ataccttttg ctagtgcttc cattaaggat agttctttgt ctgtaaagct
    19981 atccatgtat ttttctacct gtaaccttcg agtgctttta atcaaattat ctgtaggtaa
    20041 gaaaaattca tcaatagata catggagtaa tgatacaaga tcatacagca cttgtgtact
    20101 tgggtgttgt cctttgtttt ctatatttgt taaataacgt gggtcaattt caatcattgt
    20161 tcccacttgt tcacgagtga gaccccgttt cattcgagcc tctttaatcg ctaaaccaac
    20221 ggctctaaaa tcgtatttat cttctttttt acgcatacta aatcacctct atacatttta
    20281 ctgttcctat ataaccataa acaggtatag aaaaacatgt tatatagttt atgagttcct
    20341 atagaacact gtggaattta ttaatgcgat gaatccaatt tcaaatagaa aggtcgctga
    20401 ttttatttac ctatacaact ttataaaatt tagtcatact cctagaaagt agataaaatt
    20461 ggcgacttaa tggagggaaa agtcgccatg agaaagataa tctgtcatct agttctctta
    20521 attaagttct tatcaaaaaa acttatccac ctgtactgga tagatatgac catcaatgtg
    20581 gttttgcaga ttacatagat agaaaaatca aagcatatga acagagaaaa caatctgttt
    20641 atgtgctttt ttggtcactt aataaatatc ttttaggatt tctcttcatt tatgcaacaa
    20701 atcccccgtt cgtgtcgggg tttagtatga agggagataa agaagcacat gccactccct
    20761 ttcacgacag aaagggggtg aaaagcatga aaccatcttc ttttcaagaa accatagaaa
    20821 atcagtttga ctatatctgc aaacaagtta ttgaagatga acgaaaagat tacttcaaac
    20881 atctagacag actatcagca aaagaagtct cgttttctga tatagataac taccttgtca
    20941 actctttttc tactgttgat acgtacgtca ctgatttcca actatttaaa ttatttggat
    21001 taagaatcaa tgttgaaagt gatttattaa gtgaagccct gcgtcatctt tcagagaaaa
    21061 aacggaacat tattttactt cattacttta tggatatgag tgatgtggag attgcggagt
    21121 tactaaattt gaatcgttcg actgtttatc gtcatcgaat aagtggacta gccatgatta
    21181 aagaattcat gaaggagtgt aaagaatgaa aacagcttat ccaagggttc ccttccccct
    21241 cattgtaaag gcgacagatg gcgacgtgga agcaattaat cagattgtta aacattatcg
    21301 agggtatacc tcaaaacgtt cattacgtcg catgacagac gaatatggaa attctcatat
    21361 ggttattgat gaaaccctac gtggcagaat ggaaacgaaa ctcatctcta agattttatc
    21421 gttcgaaatt aaatagtccc attttttcgt ggaagcgtgt cgaatcgtat ccacgcttcc
    21481 cgatttggga ggtttgttat tccatcaaag catattgtgt gttcagtagg ttttgatagg
    21541 ctaacaagcc aaacgttctt tgacaaccaa ataaaggcaa tcgaatacgt ttcggtaaga
    21601 aaagagctga tgaactaaca cgccatgacc tacaaaatta gtgagcgatt catgttagta
    21661 attcgaaagt aatctttagc aggattgctg ccatgacttt cttatcgtga taatgatact
    21721 cccgtatagc catagtccga gcgttagaag cgtcacaggc aatgggtacg gctacatgag
    21781 aaccatgcag aagtgaaatt cctgtgagct ttgctagagc tgttcgattg ctaagatatt
    21841 cttctcttgt cagtgtatta ttataaaact atttgatata attataggtg aatattggag
    21901 ttttcctagc ctataattgg gaaaggagta ataatgataa cggtcacaaa aaaggattta
    21961 gttgagctag gatatggaac ttctttttca gcagacatca taaaaaaagc aaaagaattg
    22021 atgattacaa aagggcatat ctactatcaa tctcgaaagc tagatagagt ccctcgtgaa
    22081 gctgttgaag aaattctagg aattcggttt tcaaatgaaa acaggtctga gtagtttttg
    22141 cacagtaagg agtaaaaact atggttaaag acccaataaa aaaagcaaag aatggaacct
    22201 attattttcg tgcaaatcta ggttatgatt ctaa
//
LOCUS       DAYYFU010000237        16592 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.guided.055, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000237 DAYYFU010000000
VERSION     DAYYFU010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.guided.055"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(115..1071)
                     /locus_tag="ACHA34_003073"
     CDS             complement(115..1071)
                     /locus_tag="ACHA34_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFM8793606.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1175..1777
                     /locus_tag="ACHA34_003074"
     CDS             1175..1777
                     /locus_tag="ACHA34_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8793607.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1865..3364)
                     /locus_tag="ACHA34_003075"
     CDS             complement(1865..3364)
                     /locus_tag="ACHA34_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFM8793608.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3380..3904)
                     /locus_tag="ACHA34_003076"
     CDS             complement(3380..3904)
                     /locus_tag="ACHA34_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFM8793609.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4202..4951
                     /locus_tag="ACHA34_003077"
     CDS             4202..4951
                     /locus_tag="ACHA34_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8793610.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4942..5727
                     /locus_tag="ACHA34_003078"
     CDS             4942..5727
                     /locus_tag="ACHA34_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8793611.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5696..6412
                     /locus_tag="ACHA34_003079"
     CDS             5696..6412
                     /locus_tag="ACHA34_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8793612.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(6514..7533)
                     /locus_tag="ACHA34_003080"
     CDS             complement(6514..7533)
                     /locus_tag="ACHA34_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFM8793613.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7552..8553)
                     /locus_tag="ACHA34_003081"
     CDS             complement(7552..8553)
                     /locus_tag="ACHA34_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFM8793614.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
     gene            complement(8830..9978)
                     /gene="nagA"
                     /locus_tag="ACHA34_003082"
     CDS             complement(8830..9978)
                     /gene="nagA"
                     /locus_tag="ACHA34_003082"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFM8793615.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(10054..10890)
                     /locus_tag="ACHA34_003083"
     CDS             complement(10054..10890)
                     /locus_tag="ACHA34_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8793616.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(11074..12291)
                     /locus_tag="ACHA34_003084"
     CDS             complement(11074..12291)
                     /locus_tag="ACHA34_003084"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8793617.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(12440..12601)
                     /locus_tag="ACHA34_003085"
     CDS             complement(12440..12601)
                     /locus_tag="ACHA34_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793618.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            12674..12757
                     /locus_tag="ACHA34_003086"
     tRNA            12674..12757
                     /locus_tag="ACHA34_003086"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12708..12710,aa:Leu,seq:gag)
     gene            complement(12997..14367)
                     /locus_tag="ACHA34_003087"
     CDS             complement(12997..14367)
                     /locus_tag="ACHA34_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFM8793619.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            14460..14809
                     /locus_tag="ACHA34_003088"
                     /pseudo
     CDS             14460..14809
                     /locus_tag="ACHA34_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(15081..15371)
                     /locus_tag="ACHA34_003089"
     CDS             complement(15081..15371)
                     /locus_tag="ACHA34_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8793620.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(15579..16271)
                     /locus_tag="ACHA34_003090"
     CDS             complement(15579..16271)
                     /locus_tag="ACHA34_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8793621.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         5027 a         3313 c         2762 g         5490 t
ORIGIN      
        1 tcatgtttgt tgaatattgt gacttgtgac cgaggagttg acaggagaac accgtttaaa
       61 gaagaggttg tgaaatcagc tttttgacct gaatattagc tgaattcaca acctctattt
      121 ttggattttt tgttgggaga attctgcctt gatttcaata aaacttttaa aagactgcaa
      181 aaactggaac aattctgctc gatcctcgaa ttcagggcga ctagtcggca gaggcatttg
      241 ccgatacaat acataaactt cttcaatacg tgttaaaatt tgtctgccat catttgactc
      301 tgaaaatgtt tctgcagtat aaatcaataa accatagata tgttttccat aataagctgg
      361 ttgctggatt ctctctagat tttccagcat gtctttaatc acattggcct gtgctcttct
      421 catagagaag taagtttcgt agtatagcgg ctgtctgagc cagtagtttt cttgatgttc
      481 tctggcatct tcttggcttt cgcgaatgaa tgtcagtaaa tgttcgcatt tgatctgcac
      541 atcttcttta ttttctgata aaagaaattc tgccatttgt ctcaaaatat ttcgaaattc
      601 tttttcgatc tgtatttggt tttccttcag ctgcttcgta cgatctggca tgtaaacatt
      661 tgcaagcaaa gcaaatacta atccaatcgc cattaatccc gcttcattgc cgatcagctg
      721 ccaagacatg ttttcctcca ctaaataatg agtgaccaaa accgaattta ctactatccc
      781 ttcggttaat ttgaagcgag aggcaagcgg gatgaacagg aataaaaaaa gactgaagcc
      841 aaaaatagta tacccaacag caaaaaaact gaacagtgct gttgccgtag ctaatataaa
      901 ggctgcgatt cgattcatgc ccgtacgcaa tgtagagcgc ttggtatttc ctacacttaa
      961 taatgcaatg atccctgcag ctggccaata caacagcgat aagctacccg ctgtgaccat
     1021 cgaaaagaag acactgatca ctgttttgac tgttcgcatt cctatacgca tttacttccc
     1081 ccttttttat tttaattata cttgaataat gaaaaactag ctatcagaca aaagtttagg
     1141 tatgatagta aaaagaccaa aaaggagaat ctctatgcaa tttggccgtt ttcgactagg
     1201 tatgcgtacg atcaaaagtg ccttagctgt ttttttatgt atcttgtttt ttcatttgac
     1261 tcaacgagga atcccgatga ttgctgcatt atctgctgtt ttttctttgc gtcaggactt
     1321 gactacaacc gtttcttttg gaaagtctag gatcatcggc aatacattag gtggtacatt
     1381 agctatcatt tatttttttg tcaaaagcta ttttcacaat gattttttgg tagaattgat
     1441 cttgcttcct attctagtta ttattgtgat cgtcgtgtca gatggtatta acaataattc
     1501 cggtatcatt tccgcaatag ctacgttgtt gctgattgcg ttgagcatcc ctcaaggcga
     1561 atccgctttg tatgctatcc aacgtgtact agatactttt atagggacat tcattgcaat
     1621 cggtatcaat ttctttcttc gtcctcctga aacagaaaag aaggaagaac tatcagaaga
     1681 tttggtcgaa ctccaaaaga aagaacgatt gctaagaaca aatttacaag agatagaagc
     1741 taagatccaa aaacaaaaga aaaatagcga ggactaattt tcttttgcaa taaaataaaa
     1801 aaatggagag aagaacttta aaaagtcttc ctccattttt gattgaacgg tgaagtgtac
     1861 tactttattc ttccttcatc gagttttgtt ctgcctcttt tggcgcttca tacatattta
     1921 cttggctagt agtcccacgt gtcgacaaat tctctacgat ttccttcaag tcaagtcctg
     1981 ttgtcgcttt tagtgtttct tgagtagaag aaagcaaatt tgtggcataa tttgtcactc
     2041 ggttcgctcc actggtttct cctgaactgc ttcctgtatc gacaacagag attttttcga
     2101 tatttcctag cggctgtgct gcttcgcgca tcaattgcgg tagcatatcg ataaccatac
     2161 tcaagacagc tgcttcgcca tattccttga aggcattggc gatcttttgt tttgcttcgg
     2221 cttctgcttt tccttttgct agtgctgctt cggcttctgc ttgtccagct aaacgtgtct
     2281 tattggcttc cgcttctgcc agtgcttcca cacggaatcg ttctgcttcg gcttcagtca
     2341 cttctttgac tttttgagct tgtgcttctt gttctttagc gtaccgatcg gcatctgctt
     2401 ttttcttcac ttccgaatca tattgctttt cacgacgtgt gatttctttt tcttccagtt
     2461 cgatttgttt ttggcgttct acgattttaa tctgcatttc ctgttcgatt acttgttgtt
     2521 gtgctcgggc actttctaaa ttgtaggctt ggtctgcttt tgctttagca acatcttgtt
     2581 cttgtttgta ggttgctaat ttcagttctt tttcttttaa tgattctgcg atctctgttt
     2641 gtcgctgtaa ttcagctgct tgtgattctt tttctgcttg agcttttttg atacgcgttt
     2701 cttttagagc ttccgcttct gctatatctg catctctttt gacttgggcg attcttggtt
     2761 tccctaaaga gtcaagataa ccgtttttgt ctctgacctc tttgatagtg aaagaaacga
     2821 tgatcaaccc catttttgca aggtctacag aagcgacttc ttgtacgctt tgactgaact
     2881 tatcgcgatt ttgatagatt tcttctacag tcatagaacc aagaatcgag cgcaagtgtc
     2941 cttctaatac ttctcttgct tcattttcca gttcttctct tgtcttgcct aaaaactgtt
     3001 ctgctgctgt agcaatctct tctacagatg aaccgatttt gatgatcgaa gtaccatcac
     3061 acatcaccgg tacgccttgt tcggtataca cttcaggggt ggagacgtcc aatttactcg
     3121 ataatagact gatccgattc gaacgttgaa aaactggcaa gacgaatgcg ccacctccac
     3181 gcacaatttt gattttgtta ttgctttcat caatgtgcac atttttgctt cctaaataac
     3241 ttccactgat gatcaacgct tcatccggtt ttgctgtctg gtacttcgct acaaagacga
     3301 tcagcaacat cagtaaaatc aaagctgcga ttgcgatcgc gataaaaaca actggtattt
     3361 ccatttaaca cacgctcctt taagctttta tcatgctttc ataaggcatg acataacaga
     3421 ttctttcctt gatctcaatg atcaatactt tcgttccccg ctcagctgtc tgttcttggg
     3481 ggctgtaaaa acaagctgga cgactaatgg aaccggtaac acttttcaac tgaatttctc
     3541 ccatacctcg tacagggatc ggtgtgatca caacagctac ttgtccttcc agagtttttt
     3601 cagaagacga catcgttgat tcggcatgtt tcatcggcat caatatatag aaattaagca
     3661 aaaataccaa cactatcgat atgcctccac tagcaataaa taaaaataaa gatgattgat
     3721 ctgttaaacg ctcacctaga aaaccaaata agcttgtgaa agttaaccaa gggataataa
     3781 gcatcggatc aagcgggcca tcaaaatcga atacatctcc aaaaataatc aataggactg
     3841 ccaatcctgc acaaatcagc aatgtatacc aataaatcgt ctcaagtgcg attcccccta
     3901 tcatatacac gcctccttcc atttctacgt ctatcatacc aatgtttgaa aattctgtat
     3961 agcttctttt accgactcag tggtgttttt tataggaagg ttgtgtttat cgttgacaat
     4021 ttgttaaatt tttgttttca tgttaaaatt ttcacaataa attctttcga ggtagcctat
     4081 gtttcctttt tttaagaaaa aaccattaaa gctccagaac acgatcatcc ttgtcgtttg
     4141 tttctccaca ttcgtttctt tgattgtttc tgccatattg atcaggaatt ttattctttc
     4201 gatgaaccga tctagtgtgt cagataaaat ctctagcatt gctaaaatcg ttgccaatga
     4261 taaactcatc caaaaaaagc tgcagactgg tgacccgaca catgcgattc aaggaataac
     4321 aaatgaaatc atgattgcat cgaatttaga ttttatcgtt gtcatggacc ctgaaggcat
     4381 tcgatattct cacgcagacc caagttacat cggtcttcct ttctcaagtc atgaagatgt
     4441 gaaaaaagtg attggaggac aagaattttt ctctgaaaaa gaaggaatgt tagggttcgg
     4501 cttccgctat ttcgtcccta tttattatga tcaagagatt attggtgcag tttgtgttgg
     4561 cttaacaaac aacacgattg acaatcaagt caaagatgga caaaaaatga ttgttttttc
     4621 attgattatt ggacttagcg taggcatcat aggcgccgtt tttcttgccc aaaaaatgaa
     4681 aaaagtactg cttggacttg aaccacaaga aatagcagaa cagttaagtg aaaaaacgat
     4741 tatcgaaaat gaagtagatg aaggaatctt agccatctct tctgataaaa ctatcctctt
     4801 gatcaataca cctgcaaaaa acattttcta ccaaacagag cagctattgg atttcgaagt
     4861 aggaaaaaaa tcagtgataa tctatatgac gttttgttta aagagacgat tgagcatcaa
     4921 acgaccgttc ggactcaatc catgtattta aatgggatag aaactatttc tagcgttaca
     4981 cctcttttta gcaatgaccg ttttttagga gcagtcgcaa ctttacgaaa tcgatcagaa
     5041 gtaatgaaat taatggagga gcttgggggg acgaaacaat atataaactc catgagagct
     5101 caaacccatg aattcatgaa taaacttcat gtcatttctg gtttgattga tttgaagaag
     5161 tatgaagaag tcacccattt tatccagcag ctaaatcaaa attataaaaa agagattggc
     5221 caagtctcaa gtctgatcaa agtacctgca attgccggtt ttttattagg caaaataaac
     5281 gaagccaaag aacaatctgt tgaaatgctg ttagaaagcg attcttttat ccctaaaatg
     5341 gagatggatg actccatcca ttcacttttg caagtgttag ggaacttact tgataatgca
     5401 aaagaagccg tgctacaaaa acatgaaaga aacggacagg tccggattca tttaaactat
     5461 gacacagaag ctgaagtatt catcgtgacc gtctgcgatg gcggtgtcgg gatatcagaa
     5521 gaaatctcag aaaaaatatt tacgctaggt ttttcgacga agggaatcaa tcgtgggttt
     5581 ggattgaatc tcatccattc aatcgtcaaa cgccacggtg gatttttaga attttccaaa
     5641 caaacaaatg gcggtacaat cgtctatcta gaattaccaa aaaaggaaga tcaaaatgat
     5701 gacaaatgtc ctgataatcg aagatgatcc aatggttgca gaattgaatc gtacttttgt
     5761 ggaaagcatc cctaatatga acgtaatcga aagtttcaga aatgtagaag aaggaaaaaa
     5821 atatttcaga caaaaaaacg cagatctgct gctgcttgat gtatacatgc ctggcgaaag
     5881 tggtatccat tttttatctt ggcttagaca gcaaggaaat gatatcccag tgattttgat
     5941 cactgctgca gatgatatcg aaaccataaa gaatgtgatc cgctacggtg ccttagatta
     6001 tttgatcaaa ccgttcactt tgcaacgttt tcagctggcg atcgaaaagt atcttgcttt
     6061 ctctcagaca atccaaacga ctgaaaaagc cgatcagaaa ttgatcgatc aagtattcaa
     6121 ccacaccact cccattcacg aaataccatc attcatcgat ttgcctaaag gtctgtcaaa
     6181 actgacttta aaaaaaataa gtgcatttat tctaaaagaa gatactattt tttcaacaaa
     6241 agaactggct caaaaaagct cactgtcacg tatatccgtc aagaaatatt tgaatttttt
     6301 agtagaacaa gatatattga acgaaagaat caaatatttg gaaatcggcc agccgctaac
     6361 ctgttatgaa ttgacaaaga atgggcggaa aaagctcaca gattactgtt gagtcatcga
     6421 attatcccat acaaaatagt ccaagtatac agaagtaaca actgtttact tggactattt
     6481 ttaaacattt tattgacgaa tatccatgtt ttcttatctt aattgacggg ctttaggaag
     6541 agatacctca tttttcgttt ttccagtttc tattatttca cgaaagttct cgaaacttgt
     6601 cgaaatcata tttgataatg cctcatctgt atttgaacct acatgcgggg ttacaagaac
     6661 tctagggtac atttctacta attgctggat agcagggtct ggaatcgtct cccatggctt
     6721 gaaggatcgg aaaaaaacct ctgtttcatt tgcaaaaaca tcggttgcaa aaccagctaa
     6781 atgattgctt ttcaaagctc ttaaaatagc ttcattatct tgaagctctc cacgggctgt
     6841 attaatcaag atgctacctt ttttcatttg agctaaaaat ccttcattga tcatttgatc
     6901 gttttcacct gggatatgag gtacatgaat actgacaata tcgcttttgg ctaataactc
     6961 ttcaagcgag tggaacgtca atactttttt agcagcttct gtctgatgaa tatcgtagcc
     7021 gatcactttg gctcctagtc ccttaaacaa aacagcttcc gtcaacccta tcctgcctgt
     7081 tcctatgatc cctactgtgc aatttctgat ttcacgacta aacatttgct catccactcg
     7141 aaaatctttg aaagatgttc ggatcgtcgt atacgcagta tttctcaaaa gcatcatggc
     7201 aaaagtcaaa gataactctg ctatcgcatt tggcgaatac gatggtacac gggctactgt
     7261 cataccaaaa tcagctgccg cttgtagatc gatatgattg actcccacag tccgtgtaaa
     7321 aacataacga atcccatatt cctgcatttt tgcaagattg gtctcatctg ctatacaatt
     7381 tcccctcaac aatacagcat catggtcctt tgctgacacg atattgtcat gtgtcaacag
     7441 ttcttcataa agactgagat cataatggta aatattcaat ttttcaaaaa aatctatctc
     7501 gttcggacga acaccgtaac atgcgattct catactttct tcctccattt cttatgaaaa
     7561 taaacctatg ccatgaatca tttgtaatac aacgatccat acaggtgtca gtaccacagc
     7621 aaaaatagtt gacagtaagg atgcattgga ggctaaaagt gcctccctat caaaactgat
     7681 agcataggct gctgccactg ttgcagctgg tgtagccatc atgataacga ttgttgccag
     7741 tgcttcaaaa ttgactggta gaatattcgt tgccgtcaaa actgctagaa tcacgatatt
     7801 caatgcaggt acaaatacca ctttgactag actgtaatac catgaagttt tttcagatgc
     7861 agcttttttg aagcttactt cacctaatgt tgcgccgatt gctagccacg ccaatggtga
     7921 acttaaacca gctaaataat ccatcggctg aaatagccac ggtgcagtct ggtcaattcg
     7981 caaaaacgct acttggctta tctctccttc tgcacctgta actgacactt gtggcagata
     8041 cccttggaac agccaaatga aaaggccggc aaaagtagcg ataatgattg gattgacgaa
     8101 catcgctttg atatttttag cttccatctt caagccactc atttttatgt atccataaga
     8161 atacaaaaag attcggtagc caatattgaa aattgaggaa tacatcacgc cgacaggacc
     8221 ataaatcgca ctgacgatag gcgttccgaa aaaggtcgta gaaccaaaga tcgtcaggac
     8281 acgcaacgtt gtttgcttat cccctttata gcttaagaaa aaaggttttg aaactagaat
     8341 aagaataata taaataacta atccccaaat caagacattg attccttgca tcagtaattc
     8401 tgtattgata tcttgcatga aagctcgaaa tgccagtgct ggcaaagcca ctgttaagac
     8461 gactttcgat agaatttttc caacagattc agaaaaaatc cccttttttc tacaaacaaa
     8521 ccctaataat ataataaaaa ctgttgaagt gattgcactg acaatcgaca tatctgtcaa
     8581 agtcgatcga aaaatgtcaa ataagttcat ctttctcctc ctaattatgt tcatttattc
     8641 acaataaaaa aagcttttat tttgctaata cgtattaaca tttttaattt tatctttaca
     8701 ttgttactta ttgaacaata ggaaattaaa gaaacttttg aaacttatgt aaagtgattt
     8761 ttactagaac acaaaaaaac cttaaaacca aacttaagaa gtttagtttt aaggttaact
     8821 taaattatat tatgcgtgga aacgttccac gccatctaga taagttgctt ccaatgtcat
     8881 atcagggttc aatacgataa agtctgcatc gcgtccttga gcgatttttc cacatgtatc
     8941 atcgatttta cagctgattg ctggaatcag actagccatc atgactgctt gttctggtgt
     9001 tgctaatccc cagtctacaa catttttaat tgcttctttc aatttcaata tacttccagc
     9061 taagttgcca gattctagac gtgctgttcc ttcttttact actactggaa attcgcctaa
     9121 attgtaattt ccatcaggca tcccaccggc catcatacag tctgtaatta atgcgacatg
     9181 atcatgtcct gctttttcca ttaggattcc cgcagcattt ggatgtacat gatggccatc
     9241 acaaatcaat tcagaaaaga catgttctaa agataataac gcacccacca tacctggttc
     9301 gcgatgattc aatccacgca taccattgaa agcatggaca aatacacttg cgcctgcttc
     9361 aacagctttt tgcgcttctt ctagtgtagc attactgtgt cctagagaaa caactactcc
     9421 ttcttcagtc acttgtttga caaactcttc tacaccttga cgttccggtg ctaaagcaat
     9481 ttttttgatt aaaccacctg aagcttcttg ccattcatgg aaagtatcca aatcaggatc
     9541 cccgaaataa cttgggtttt gtgccccttt gtgttcttct gtgaagaaag gaccttcaaa
     9601 atagattcct ttgattttag ctcctggtgc ttcctgatac attttgccga ttgtctcagc
     9661 tacatctttt aaacgttctt tgcttgacgt caatgttgtt ggtaaaaatg aagtcactcc
     9721 acaagataac agaccttcag acatcacttt gatgccttct gcatcattat ccattacatc
     9781 atgattcata tagccgtgaa tatgtgtatc taccaaacca ggagcaatcc atttgccgct
     9841 ttggtcaatg atgttgactt ctccctcagg tagttctttg gtgtattcac caaaaagtcc
     9901 atcggtaatt tcaagatagc ctgcatgttt gacacctgac gctaaaaaga atttgtcagc
     9961 atagataaaa gttctcattc tattctctcc ctttgttttt ctacctctaa agtactctta
    10021 tttagagaag aagtcaagaa tggtctacac catttacaaa ttttttgaaa tttgtttcac
    10081 agcttgtgct aagccttcat agaatgctgc tctttctggt tcttctttac gcttgtatat
    10141 taagctttta agcacaaata aatcattgag gtaatagtag ctattcttct cttgagccca
    10201 atagattcct tgattcacct gttctaacgc ttcgtcgatt tcattttgat ggaataagaa
    10261 atttccataa ttataaaaaa tttgcgaaaa ttcacttttt gatcgttcat cagctgattg
    10321 atgcagcagt tggatgcttc gactcaaata gtagcgtgct tcagccgttt ttcctgccac
    10381 accatatatt tttccgatac aactaatcag ttgtatttct gtgtcagaaa catggattct
    10441 ttctttttgg ctagagtagc ttaatgcttc tttcaaataa taaagggact gagagatatt
    10501 ttgacgcaac gtgaattcgc aaacaccgag ataataataa tagcgttgaa agtcagttgc
    10561 ttgatacaga ttttcagaaa tatcagaaga ttttagtact tgttctaact tccgataatc
    10621 acgtttttgg aaacaactat ccaacagttc aaataattta tcgtttgtcc gagtagtttt
    10681 tcgagagaga ttcatcacat gatcaatcgt tacccctagc cgttgacaaa tctgcatcat
    10741 caccaataca ttcggcagtt cctcattatt ttcgatccga cttaatacac tttgtgaaca
    10801 aatatcatgc gaaagcattt tttgcgtcat tttttgttct tttcgaatct ctttaatgat
    10861 cgttccaaag gactcgatta acgtttccat aagtatcctc cccaaaatat gcgtattcgc
    10921 attctattcg tattttactt taggaagtcg caaacttcaa gaaaagactt gcaactaaat
    10981 aatatacttt agttatttaa gaaaactcca ctagaaagag agcctcagtg tgtttgtctg
    11041 tggccctcct actgaaaaat ttggttttta aaatcatttt tggcgatagg ttgctaggaa
    11101 ttctctcatt ttcttagctg tttgtttcaa atcatccatt ttaggaagat aaacgattcg
    11161 aaaatgatct ggctggttcc aattaaatcc accaccatgt acaagtagaa tatgatgttc
    11221 atgtaaaaag tccaaaacga atttttcatc atccaaaata ttgaatcgct ttgtatcaat
    11281 ttttgggaat atataaaaag cagcttttgg ttttacggct gaaagaccag gaatatcatt
    11341 gattgcatta tagatatatt ctctttgttc atagatcctt ccgcctggca ggagcagctc
    11401 atcgacactt tgataaccgc ctagcgcggt ttggataatt tgctgagaca atacattgga
    11461 acacaaacgc atggaggaca acatattcag cccttctata tagcctttga cgtgtgactt
    11521 atctccactt aggaccatcc agcctactcg aaatcctgct acacgatgag atttggatag
    11581 accgttcagc gtaacaacga acaaatcagg cgctaatgta gcaatcggaa tatgttctag
    11641 accatccatc actaagcgat catagatttc gtcggaataa ataatcaggt tattttgtcg
    11701 agctacttca acgatttctt ctaataattc ttttggataa agtgccccag taggattatt
    11761 aggattaatg ataacaatag cttttgtccg actcgttatt tttgatttga tatcatcaat
    11821 atcaggatac cattctgcct cttcatcgca aatataatga acaggtgtgc ctccagctaa
    11881 tgcaatagaa gccgtccaca acggatagtc cggcattgga accagtactt catctccatt
    11941 gtccagcaac ccctgcatac acatcgtgat caattcgctc actccgttgc cggtgtagat
    12001 atcattgatc gtcacattcg gaaaatgttt cagttggcaa tattgctcaa ttgcttttct
    12061 agcagaaaag atccccttgg aatctgagta gccttctgag tcacgggcat tcatgatcaa
    12121 atcacgaatg acttcgtttg gtgcttcaaa tccaaaaggt gcgggatttc ccgtgttcaa
    12181 ttttaaaata ctaatgccct cttcttgcat ccgctccgct tcttctagaa cgggaccgcg
    12241 aacatcgtaa ctgacacctt ctaatttgtt ggatttttca aattctctca tcacttagcc
    12301 aatccctcat ttcaaaaagt tagaactttc caaatttttt gttatttact atttcaatat
    12361 catactccat cctagaaaat tttgccactt atttaggaaa aacgtaagta ttaaaaaaac
    12421 acaaacaagc gttgagaact taattgtcat gatagtcatt ccatagattc gctgcccaat
    12481 ccattgcaat tccacaaatg attcctagca taaaaccaag caaaaaacgt ccgaatgcaa
    12541 gtccaatcaa tacacctaag atcattccca gtcctgaaaa ttctccgtca tatttctcca
    12601 tcttaataag tcctcccatt tttgagcttg acaactatgt aaaacctcgc tatcattagt
    12661 gtgttattat tacgcagatg tgttggaatt ggcagacaag catgattgag ggtcatgtga
    12721 gcgttagctc gtgtgggttc gagtcccatc atctgcacta caactcgtga agttaatttt
    12781 gcttcacgag tttttttgtt tgaacgtttc tttatgccat tatatacata tcactgtatc
    12841 ctttacaaaa agaacgtaca atcatgtaaa cagcctgagg tgagtatatg aaggtcggaa
    12901 acaagctact cgtagaaact tgcaagtgtt aaaacaatta atcgaaaaac gagtaaaatc
    12961 cctcacctaa tactaaagcg agggattttt tgtatattat ctgtttatca ccgttattat
    13021 aaaggtacaa ataccaagca cggttaatcc aaattgcata aattcatttt tttgtacact
    13081 catgcttttc attagctctt taactgccat tagtactaag atggcgataa caacataaag
    13141 tatatagtac gtccgcattc cggaagccca tactgtggga gaaaatccca taattaacct
    13201 tgaacaaaat cccattatca tcaaaataac taaaaaaata gaagttttct tattgtcaaa
    13261 acttaaataa attccaacaa ttaaacatag taataatgcc aagactacta aagtagctat
    13321 ccaagtacct ggataaaaaa gtgacagttt cgttcctaac tctgtaaaga gattattcag
    13381 gttactacta ctccaaatca ttgccctttt atttccattg acgtaagtaa agctttgacc
    13441 cattgtatta ccaaaataaa taatcaggtt taagaaaaac ggtaatgcag tcaatatacg
    13501 cacataatag ttttgacatt tcctatatgt tagaaggaaa attaagaaaa atagcaataa
    13561 aaataagata ttcatgtcta aaaaaaacgg ctttccaaat gatgaaatac ctaaatctaa
    13621 cttattaaga aagttgaaat ttttatattc aggaaaccat ttattaattt catgtgcagc
    13681 acgtaatgca ttccctggag ttgtcaaaga gaaaattaat tctgctaagc ttattattga
    13741 aaatactgat aacttataat tatatcgtcc attgacaatc aaatataaac taactatact
    13801 tgttagaaca aaaaaacagg cgttgacttg ttcttgatta gttaaaaata tcaataaagg
    13861 aatacttacc caatatattt ttctatttat ttcttctcct cttaagagtt gaaaaaacgg
    13921 ataaaaagtt agtaaactaa atgctactgg ccactgatag ttcaaggtgg ttgctctcca
    13981 accggtttcc cccattactg tcaatggtat taaacagtat atagagcaaa ccaataataa
    14041 atttttaaca tttattttct caaaaacata cttgcaaagt aatactacaa ttcctaacat
    14101 aatcgtagaa tttaataatt tccatagcat aaagtgttta gaaaagaaca ttaagaatag
    14161 ttctataata attcttgaac tccatgtctt ataatgttct atcgaagcac ccaaatagcc
    14221 aagttcatct aatctacttg cataaactac gtcatccgct ctcatagtcg gcatatacat
    14281 attaaagagt aaaaagatac cccaaatccc tattagtaac aagatcagtt ctttgttatt
    14341 cgttattttt ctattatgtt tgttcatttt tatactcctt cttatatttc ctaatagagt
    14401 ataacatagc tttaagcatt acttaaagat aacttaaaag ttatctttta aatacttaat
    14461 tgttaaatac cttatttgat gcaaaagaaa tctacgatag tcaacaaaaa aattttcatt
    14521 atatccagaa aaatttttct tagttaagga cctatggatt gcagtaatgc agaattcgtc
    14581 gaataaactg ccaaagacat acaatcatat tgcttttaaa attgacgaac aagatataga
    14641 ttcgtttgtc tcaaaaatcc aaatgttagg tttaactgtc gaaccaggac gttctcgtgt
    14701 gaaaggcgag gcttcttcaa tttactttta cgactatgat aatcacctct tcgagttaca
    14761 taccggtact ctccaggaac gtttaaaatc ctacaattcc actacctgag actagtaatg
    14821 actagtctct ttttatggcc tctgatcccc ccattgagcg aatactaata gggcttcgaa
    14881 ttgatctgtt caatctttaa gcaatatccc tcaaatgagt ttggaaagtt tagtgattca
    14941 tcacaaatac ttaaatacat gtagttatat atagattgag gttcttgtga gcgtatgctc
    15001 gtgtgggttc aaatcccatc gtctgcatcc atctttccaa aacaagcggc taggacattt
    15061 ttgtctcagc cgcttgtttt ctagtttatt ctttcatctt gtcggacctt ttttcttggg
    15121 tatagacttt tctcatattc taccacaatt ttgggtttcc ctaacacatg ataggatctc
    15181 acaacaaatt gctttctatt attataataa cctgtaacga ctaacttcat cccttcgtct
    15241 acttcagcta ggaagttcaa actatgggaa gcaatcaaac aattgacccc atttaaactg
    15301 aaacggatca acggtctggc actaacttgc aaagtacgga tcttatctac tgttccatat
    15361 tttttctcca taaccttcac ctcaatacca ttatacgaac atacattccc tttgtaaaga
    15421 agaaactctg ttttatcttt ttgacttttt gtctattacc tttcttaaaa tcatattggc
    15481 acttcctgcg aagatgaagg acatgtacta aaaaaaagag gcagcagtat agattactgt
    15541 tgcttcttta ctaatttagg tttacttgtt aagaaagatt atttacgcaa atgttcttca
    15601 acaatttgct gagatttatc agctgtatct tccacttcat cttttgcttt ttctaacttt
    15661 tgaatatatt gtttcaagct tgagttttca gcagttgctt cgtctaattt cttttgtgta
    15721 tctgttaact gagtttctac atttgttttt tcttgtgtca gtgttgcgat ttgttgattc
    15781 aaaccattta cagtattttg taattctgaa agttttgctt gtgcttcttg tagtcttgct
    15841 gtccaatctg cattcagatg attgatttca tcattttttt ggttgatctc atctagtttg
    15901 acattgattt cattttgttt gctttgtagt tgtgcatcct tgctgactaa ttgactttct
    15961 aagttttgtt tttctgctgc cgattgattt ttttggtttt ctaattcatt tacttttgtt
    16021 tggtattctt ggacatctgc ctttgaatct tgcaatgtct gttctttttg tgctagtcga
    16081 gcggtaagat ctgtaagcgc ttgctcttta tctgtcaatg tatgatcaag ctgtgtcaga
    16141 ttctcattga ttgtatcaat atctgtctct ccaccccaca aaataatttc atttgcgtac
    16201 cgcgtgcctg tgtagccaat tagacataga aatgcaacga caatcccacc agtaataaat
    16261 ttctttttca tttgcatagc tccttaatcc aatactttta catgctaaaa gtaagtgatc
    16321 tcaaacacaa tgtcaagcgt ttttaaatta catgtgtaat tttaatataa atactatcaa
    16381 atactcattt aatcctcctt ttttatctat ttaaaaaatc ttttttatag agaaaaatgc
    16441 aggttaatac tttcttaaat aaatatttat ggtgaacaaa aacaaaaaag caagttttat
    16501 taaacgtaac ccttttcatt tcatagatta ctgtatttat tacaaaaaac gcggatttgt
    16561 cataaattgt catgcttctt ttacctatga aa
//
LOCUS       DAYYFU010000238        14730 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.guided.061, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000238 DAYYFU010000000
VERSION     DAYYFU010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.guided.061"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            100..996
                     /locus_tag="ACHA34_003091"
     CDS             100..996
                     /locus_tag="ACHA34_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8793622.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1146..2087)
                     /locus_tag="ACHA34_003092"
     CDS             complement(1146..2087)
                     /locus_tag="ACHA34_003092"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8793623.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2213..3334
                     /locus_tag="ACHA34_003093"
     CDS             2213..3334
                     /locus_tag="ACHA34_003093"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8793624.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3592..5094
                     /gene="eat(A)"
                     /locus_tag="ACHA34_003094"
     CDS             3592..5094
                     /gene="eat(A)"
                     /locus_tag="ACHA34_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8793625.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5161..5688
                     /locus_tag="ACHA34_003095"
     CDS             5161..5688
                     /locus_tag="ACHA34_003095"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8793626.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5899..6960
                     /locus_tag="ACHA34_003096"
     CDS             5899..6960
                     /locus_tag="ACHA34_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM8793627.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6963..7859
                     /gene="murQ"
                     /locus_tag="ACHA34_003097"
     CDS             6963..7859
                     /gene="murQ"
                     /locus_tag="ACHA34_003097"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM8793628.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7873..9855
                     /locus_tag="ACHA34_003098"
     CDS             7873..9855
                     /locus_tag="ACHA34_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM8793629.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9920..10366)
                     /gene="nrdI"
                     /locus_tag="ACHA34_003099"
     CDS             complement(9920..10366)
                     /gene="nrdI"
                     /locus_tag="ACHA34_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM8793630.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10577..12001
                     /locus_tag="ACHA34_003100"
     CDS             10577..12001
                     /locus_tag="ACHA34_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM8793631.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12182..12955)
                     /locus_tag="ACHA34_003101"
     CDS             complement(12182..12955)
                     /locus_tag="ACHA34_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8793632.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(12952..13857)
                     /locus_tag="ACHA34_003102"
     CDS             complement(12952..13857)
                     /locus_tag="ACHA34_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8793633.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(14011..14331)
                     /locus_tag="ACHA34_003103"
     CDS             complement(14011..14331)
                     /locus_tag="ACHA34_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM8793634.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         4720 a         2617 c         3109 g         4284 t
ORIGIN      
        1 gttaaatttt catacaaata taggaaacgt tttactggct ttttactatt taaacaggct
       61 atcataggaa aaagtgacag agaaagtgag gcggaaaaga tgaacaaaaa tgacgtactg
      121 ctagttgcaa aaaataaatc actttatttt ttgccttcca tccgctcttt agatgtttgg
      181 tccggaaaag atttactaca gtcggaattc attaaatcag ctagacaaca gttggcaaat
      241 tatcagctga tcgttttttt agattatgga tttgatatgg agatggctcg acgcgtgcgc
      301 ccatttacca atgcaaaaat tgtgttattt ttttggaatc acttcaaagc agaacacgag
      361 gcgatgctga agcaggcaca agatgaaccg gcaatcgatg agatttatca ttttgatttt
      421 ttggaagcaa aagaacttgg gctgaaacat aatagttcat tttattcgaa acatatgaat
      481 ttttcttcta gcgaaccaaa gacggatcta ttttttggtg caacgaataa tgggagaaaa
      541 gaacgagcag aaagctataa gcaggaattt ataaaaagag atgttacgac caactatttc
      601 attcttccac atcgagggaa tgaacaacct ggatatctta cttatgaaga gtacctgaaa
      661 aaaacagggg aatcgcgcgg gattctggaa ctgttgcggg agggtcagca aggagtgact
      721 ttgcggacat ttgaatccat gtattttcaa aagaaactag tgacggataa tcaagcgatt
      781 gctcattatc atttttatcg tcctgaaaat atttttttgc ttcgagaacg tgatttagat
      841 gaattaccgg aatttctgca tacaccattt aagccaatcc tatcagagct attggaattt
      901 tatgatgcag aaaattgggc aaaacgattt atcactaacg attccactat ttatgagcaa
      961 tacgaatatg acctctcctc tgttgaaaac aaataaaaaa cctacatttt gaagagatta
     1021 tgtactgaaa caaacaagca caattcttat ggcatcatga gagttgtgtt ttctttttga
     1081 tcttttattg ggcttaaaaa aagagaacca gctgctgatt cagctgattc tcatcagaga
     1141 gccttttatt ctgcgccaag gattccttct tttgcatgga ctaattgggt caaaaaggca
     1201 caatatggtg tagcaatatc atatttttgt ccttttcgcc aaatggcacc attgatgtag
     1261 tcgatttcag tcaaacgatg gtttttgatc aggtcttggt acatagatgg gtaatgcaac
     1321 ccgatgttgt caggatcata agtagaagca atatgttggt acacttcttc ttgatctagt
     1381 atgattcctt cttttgctgc gatagaagca aattcagata cgattgttcg aaccatgctc
     1441 tctgccgctt tttgcgcgcc tagttcagca atattacaat ccaaaatcgt gcataaacca
     1501 ttcaatgttc catttacaca agcttttcgc caaatggaat aacgaacgtt atggctatag
     1561 atcggattta aatttgcttc ttgaaagact tctacaactt tttttgtaaa tgcttcgcct
     1621 tctggttcca agttctctag ttcgatttct ccatcaccta acaatgtgac ttttccggga
     1681 ccagctagac cagctgtcca catcgtgatc ccaaataaga tatttttctt tggcacatat
     1741 ttttccagta catcttcatg acctaatcca tttagtaaac acaaaacata agtattgtca
     1801 gtaataatgg attggatcga acaaaacata tcatctaatt gattggcttt tgttaatgct
     1861 acgatcaagt ccacgtgttc gttgctttcg atgatttctt ctggtgaata gattggcagt
     1921 tttgcgacca cttctttacc gttaaagtca gcaatcaacc catttgtacg aatggcttca
     1981 atgtgagcag gccaacgatc gatcaagagg acctcatttc cgctttgatg tagcatcaat
     2041 ccgattcggc tgcccattgc tccggcccca gctattgcga ttttcatgtt ttttctctcc
     2101 tttattcaat gagttccctt ttagtatata gaaaaatgga agaaaatagc attgttttgt
     2161 ttgaaaaaaa tgaaaaaaag tgtcttactt aagaagaaag tgagggatac agatggatat
     2221 tttggcggca attgattcat ttaaaggaag tgcttcttca tttgaactaa atcaggcagt
     2281 tctaaatggc tgggaagatc gtaaaggaag aaaaatcaat gtgccgattg cagatggcgg
     2341 agaaaatact gcagaagcaa tctattacgc gctcagggga acatggcgta ccattgatgg
     2401 gatggatctc ttattccgtc agcgaaaaat cagctattta ttgacaactt ggcaaggaaa
     2461 aaacaccgca gtcatcgaat ctgcacaagc attggggtta gatctacttg ataaaccaac
     2521 ggatcaaact atccgacttg cttccagtta tgccttagga gaacttatta aagatgcact
     2581 gcttcaagaa gtagaacaaa ttattgtcac tttaggagga agcgggtgta gcgatggtgg
     2641 tctaggatta ctccaatcct tgggtgcttc tttagaaggg tacacagaag gaaatccttt
     2701 attatctact aaaaaacttc attttgatcg aagaaacgtt cttgtttcta aagtaaaatt
     2761 gattatcgct gcagatgtta ccaatccata tacaggtcaa caaggagctg cttggcaatt
     2821 tggaagacaa aaaggaggaa tagatacgac gctagctttc ttagataaac aagcaggaaa
     2881 gattgctcag caaataaaag atttctggga ggtggattta gatgatattc caggaagtgg
     2941 agcagcaggc ggtattggtg gagccctttt tttgttggga gcgaagatgg tttctggttt
     3001 cgatttagtc agccaaatca ctggattgga aaaaaagatc gaacaagcag atgtcatcat
     3061 tacaggagaa gggcggatcg atcagcaaac gatccacgga aaagttccat acggtgtagc
     3121 agtgcttgca aaaaaatatc gaaaaccaat aatcgctatt tgcggaagta gagaccaaga
     3181 gctagaagga ctttctgatt ttctcccaat tgtcttaagt attcatttgg gtccaatagg
     3241 tttagaagaa gcaatggaac acaggcaaac attagaaaaa acaaaaatct taggacagac
     3301 acttagcaga tttttcacca tcctcccgaa gtagttgaca aaatcatttt ctttatgcta
     3361 tcgtaatttc aaatatagga caaagtgagg gatccgtgca ttgaaaaatt aagtcgattg
     3421 atgaaagctg cattcagact gtaccttgtg gagacaagga agcagtgtgc agattttcgg
     3481 gattggcttg gttttttcga tgtatgggtc tttttgtgtg tatccaaccc ttgcatcctc
     3541 tttagccagt tctgaaaata ggactggtct ttttttacga ggtgatggaa aatgtctaaa
     3601 atcgaaataa aaaatctgac attcggctac gacagccaag gcacattatt atttgaacaa
     3661 gcaaatctaa attttgacac acaatggaaa ctaggactta tcggacgaaa cggtcgagga
     3721 aagacaactt tactgaatat tctacaaaac aaactacctt atcaagggca agtaatccat
     3781 cagcaagaat ttgcctattt cccgcaacag acaaaagata aagaacgttt aacctattac
     3841 gtgttaaatg atattacgga ttttgagata tgggaaatcg aaagagagct ccaattgatg
     3901 caaacagatc ctgaaatctt atggagagaa ttcagcacac tatcgggggg agagaagaca
     3961 aaagtcctac tggcactttt atttgtggat gacactcatt tcccgttaat cgatgaacca
     4021 acgaatcatt tggatatctc tggtagaaaa caagtagcgg cttatttgaa aaagaaaaaa
     4081 caaggcttca tcgtggtcag ccatgaccgg ggatttatcg atgaagtagt ggaccatgtt
     4141 ttagcaatcg aaaaaagtca actggaactt tatcaaggga atttctctat ctatgaagaa
     4201 cagaaaaaac ttcgtgatga atttgaaatg gctcaaaatg aaaaattgaa aaaagaagtc
     4261 agtaggctaa agaaaacagc agctgaaaaa gccgaatggt ctcgttcccg agaaggagat
     4321 aaaacaaaga aacaagtcgg attcatcgat actgaatcta gacgagtgaa taaaggagca
     4381 gtgggtgctg atgctgcacg gacgatgaaa cgatccaaag caatcgtgaa tcggatggag
     4441 acccagatca gcgagaaaga aaaactatta aaagatatcg aatatatcga ttctttgacg
     4501 atgaatagcc aagcgtctca ccataagcga cttttaagcg tagaagatct tcaattaggg
     4561 tatgaaaatc tgttattcga gccaattcat tttacaatcg agcctcatca gcgggtggcg
     4621 atttcaggtc ctaacggtgc aggaaagtca tccattatcc attatcttct gggggcattc
     4681 aacggcaagg ttataggaga aaaataccag ccaaaacatc tgagcattag ttatgcaagc
     4741 caaaattatg aagacaatcg aggaacgttg gcggaatttg cagagaaaaa ccaagtagac
     4801 taccaagcat ttttgaacaa cctccgaaag cttgggatgg aaagagatgt ttttcataac
     4861 aagatcgagc agatgagtat gggccaacgg aaaaaagtgg aattggctaa atctttatca
     4921 cagccagctg aactatatac atgggatgaa ccattgaatt atttggatgt cttcaatcaa
     4981 gaacaattag aacaactgat cttgaacgtg aaacctgcca tgttactagt ggaacatgat
     5041 caaaccttcc tggataaagt atctactgag attatttctc ttgagagaat ctaacccttg
     5101 taatctcaag aacaacaagg tatggttaag gggaactttg ccaacgaagg agagaatcac
     5161 atgctttatg taacgagaca cggagaaaca acttggaacg cacaaggatt agtttgcgga
     5221 cgggcagatg tgccgctgac tgaaaaagga cagatgcagg cacagaaact agcagaaaag
     5281 gtagtagatt tgcctgttcc aatcacgaaa atcatccatt cccctcttca acgagcaaga
     5341 gatacagcac aagcagttgc tgatcgtctc agcttgccgc tgaccgttga tgaacgattg
     5401 gtagaaatgg attttgggga ttatgatgga ctgccaagca aagatgagaa ctttcaaaaa
     5461 gcacgtttag cgttcgctgt acgattccca aatggggagt cagtcttaga tgtgtatgca
     5521 cggatcgttc ctttattgaa agaatgcatc gaagacgaag aaaatgtcta tctgttagtt
     5581 tgccataatg ccttgataag agtgatcaat gcttattttc atccgatgcc taatgagggg
     5641 ttctttacat tcatggtaga taatacagaa ttggtttctt atgagtaatt atgtttttgt
     5701 gttatttaaa taaaatattt caaggcactg cttttgctta gcagtgcctt ttttaggtta
     5761 tgacaattct tttgataata taaactcaac ctttagaaat gaaatttaat tattttaata
     5821 aaagtattga aattttgttt ctttagatat atactctagt catagaaatg aagacggata
     5881 cagaaaaaga gggataatat gtttggcttt tcaatattta tgaacgattc tttgtcagaa
     5941 gagaagaaac agtatatccg ccaaatggca tccaatggct ttgtcggtat tttcacttcc
     6001 atgcatatac cagaagatga tgtttcggca tatcaaaagc gattgatcga actaggaagc
     6061 gaagcgaaaa agaataaact agaattgatg gtcgatattt caggagaggc attggaacga
     6121 gcgggttttt cattagaaga tctaacgcct ttgaaagcaa ttggtgtaac aggtttaaga
     6181 atggattatc atatttccaa tcagcagatt gccgactggt cacatcaatt gaaaatcagt
     6241 ttgaatgcta gcacgatcac gcctaaagat atcgatgaat tgaaagaagc agaagctgat
     6301 ttctcacaga ttgaagcttg gcataattat tatccacgtc ccgaaacagg gctggacaaa
     6361 gaatggtatc agaaaaaaaa tcaatggctc aaaattcaag gtctccttgt ccaaggtttt
     6421 gtaccaggag atacagagtt gagaggccca ctatatcaag gattgccaac tttggaagaa
     6481 catcgaggga tccatcctct tgcaggcgct ctagatctat tagcaagtaa cacggatatt
     6541 gtttacatcg gagatgcagg tctgtcagaa aacgttcagg agcaatttgc ttctttccaa
     6601 aaagaacaga cagtcttgct ccatacggaa cctgtagatg aagaatttta cgaatatata
     6661 ttagggaagc atacgaatcg gcaagacgat gcaagagatg tcatacgaag cgtagatgca
     6721 cgttttcgag agattccgcc tatcccagca agaaatacag ctacgcgtat gaaagggtct
     6781 attactttag ataacgaaaa gtatttgcgc tatatgggtg agatccaatt aaccaaatat
     6841 gatttgccag cggatgaaaa agtgaatgta gtagctaaag ttatcaaaga agagctacca
     6901 ttgatcaacc aaatcaaagc tggtatgaac tatcaattta taagaaaaga aggaagataa
     6961 aaatgagcaa aatcaattta gagaacctaa caacggaacg cagaaaccaa gaaacttttg
     7021 gattagatga gatgagtatt tctcaagccg ttcaactaat gaacaaagaa gatcagaaag
     7081 tggccgctgc ggttgaaaaa gaactgacaa gtatcgaacc agtgattgct aacacgattg
     7141 agtcctttaa aaaaggtgga cgtctgattt atatgggagc cggaacaagt ggaagactag
     7201 gtgtgttaga tgctgctgaa tgcgtaccga cattcggtgt agaaccggaa atggtcatag
     7261 gtctaattgc tggtggagaa gaagcaatga ctgtagctgt cgaaggggcc gaagattctt
     7321 atgagctagg gcaagaagat ctaatcaagc tgaatttgac aaaaaatgat atagttattg
     7381 gtatcgcagc tagcggtcgt acaccatatg tgatcggtgg attagattac gcaaaatcga
     7441 tcggtgctgt cacaggaacg atttcttgca ataaacaagc cgaaatcagt aaacacgcgg
     7501 atttcccaat cgaagtcgat gcaggtccag aatttttgac aggctctact cgattgaagt
     7561 caggaacagc acaaaaactt atcttgaata tgatttctac tatcagtatg attggtatag
     7621 gtaaagtatt caataacttg atggtcgatg tcaaaccaac aaatgaaaaa ctaatcgaac
     7681 gctcaaaacg aatcatcatg caagcgacag aagctgatta cgaaacagct gaaaagtact
     7741 ttgaagaagc agatcaaaat gttaagctgg ctatcgtcat gattttgaca aatagtgata
     7801 aagaggaagc tcaagcaaaa ttagctgaag caaacggatt tgtaaaaggg accttatgag
     7861 ggggaaaaga ccatggcaaa aaacgaactg actttggaag aactggcaaa acaaatttat
     7921 aaaggggcag gcggcatggg caatgtagaa agcattgtcc actgcatgac tcgtgtgcgt
     7981 atgaagatca tcaatgatga tcttgtaaat gtctctgaat taaaagcgat tccaggagtg
     8041 cttggtgtag tagaagatga acagcttcaa gtcatcatcg gacctgggaa agtgaacaaa
     8101 gtagcacagc aaatggttga tttggcaggc gtgaaattag gagaaaaact ttcttctgta
     8161 agtacagcta cagcaaattc atcagcatct ggaaaagacc gtgtaaacga acgggcacaa
     8221 gaaatgaaag cggcacaaaa atcgaaacaa aaaccatcta aaatcaaatc ggtattaaaa
     8281 gacatttcta atatctttgt gccaatgatt cctgcatttg tcggtactgg gatcgtggca
     8341 gggattgccg cagttttagc aaacttagtc acagccggaa cacttgatgc agcaacatgg
     8401 caacaatata tcgatgtgat gaacatcttg aaaaacggga tgtttagcta cctagttatc
     8461 tatacaggta tcaatgctgc acaagttttt ggtgcaacac caactcttgg tggagttatc
     8521 ggggccgttg tgatgctgac agggatgaat ccggaagcac cactgaaaaa cttatttact
     8581 ggtgaagctc ttgcagctgg acaaggtggg atcctaggtg tcatcttcgc tgtttggcta
     8641 ttgtcaatcg ttgagaaaaa actgcataaa gtagtacctg atgcggtaga tatcatcgtt
     8701 acaccaactc tttcattgat tgccatcggt ctgattgaaa ttttcttcat catgccttta
     8761 gcaggtttca tttctgatgg catggtcggt gggatcaact gggtacttgg cgttggtgga
     8821 gcatttgccg gcttcgttct aggcacatta ttcttgccga tggttatgtt cggactgcat
     8881 caaattttga ctccaatcca tgtgcaaatg attgatgaaa ctggtcgtac gctattgctt
     8941 ccaattcttg cgatggctgg tgcaggacaa gtgggtgcag cattggcatt atggattcgc
     9001 tgcaaaaaag ataaagaatt aacagaaatg atcaaaggag ctttaccagt cgggatccta
     9061 ggaatcggcg aaccattgat ttacggagta acattacctc taggacgccc atttattact
     9121 gcttgtatcg gtggaggtat cggcggggca gtgatcggaa tgatcggaaa tgtcggggct
     9181 atcgctattg gaccttcagg agccgctttg atcccattga tctctgacgg taaatggtta
     9241 ggctacatcc ttggattatt ggcagcttat gctggtggct tcgttgctac attcttcttc
     9301 ggtattccta aagaccgttt agaaaaagaa gaattagcga ctgaaacagc cacagtaaat
     9361 gaagtgcctg aaaaagtagt acctcaaact tcagctgaaa gtgtgactat cctctcatca
     9421 gttgctgacg gaacagttgt ttctttagat aaagcaagtg atccagtttt tgcacaaaag
     9481 atgatgggtg aaggctactt tgtagaacca gaaaacggac atatctattc tccagttact
     9541 ggggtagtca gctctgtctt cccaacgaaa cacgctattg ggatcacaac gccaaatggt
     9601 ttagaaatct tgcttcatat gggaatcaac acagttgact taggcggtac gccatttgat
     9661 gtgaaagtaa cagaagggca gcaagtgaca cctgatacgc ttgtagcaga agctgactta
     9721 gaagcgatca aagcagcagg caaagaaaca tccatgatcg tcttggtaac caatatggat
     9781 cgtgtaaaaa actttgtctt ggaaaaaaca ggtaaagcaa aagcaaaagc acctgtgatg
     9841 gatgtcaaag cttaatatga tagaaacaat agaaacgaaa gaagaaactg aaatctattt
     9901 ttcagcttct tcttttttat tatttattgt tttctgctaa aatctgataa attcgtgtca
     9961 catcagatgg tgttccccgt aattcataat ctgccaagaa aggaaagcca aatgtttgtg
    10021 catattgttt ggctgttaga caatactgat tattgaagtt tttatttccg ctaccgacta
    10081 caccaagaca aagtttatga ttgtcttcat gttccagata ttcacgcatc gtttcggtca
    10141 agatttctgt atcaccgtta tctaaaccat ttccgccgtc taaataagta ggaacgaaag
    10201 taaaaaatgg ttctgtttct ttcgtaaaat cactattttc atggatttct tttaaagtaa
    10261 cttcaggatt gacaggatct tctgcatgca ttttttcagc atattctgct agatgtttgg
    10321 caaaggcacg cgtatttcct gaaatcgata tatataaaat gttcatggat catcctccaa
    10381 ttctttcctt ttagtatagc atctttttta agttttttgg tggtgtaatt tggaaaggga
    10441 ctaggcgctt tgcttgtata atgagaaaaa agccggtaga attatatttc tattaaagtt
    10501 tttattaatt aagtaaaaat aaaaaaggtt tgttatactt tttgataaaa gcggttttat
    10561 tttggaggat gcttacatgg atacagagca acagaaaaaa ggaattggtt taattccact
    10621 tgctgcctta gtaattggtt cagcgattgg aggtggcgta tttggtatta tgacggacat
    10681 ctccggatcg gctggcggcc cagctatttt cagttgggta ctcgtaggaa cagcaatgtt
    10741 gatgctctcc ttgtcgatca ataatatcaa taaaaaacgt ccagatttaa aaggcgggat
    10801 ctttagttac gcagaagaag gttttggccg tttcgctggt tttatcagcg gttggggtta
    10861 ctggctcaca tgctggttag gcaacgtagc ttttgcaaca ttgttgatga gcgcattagg
    10921 atatttcttc ccagtatttg aaggtgggca aaatctggca tcgatcgtga tgagtacagt
    10981 ctttttatgg ggatatgcat ggctagtgaa tcggggagtc gaaaatgcta gtttcgtcaa
    11041 tgctatcgtc acattctgta aattagtgcc attattttta tttattgtag tagctatcag
    11101 cacgttttcg atccaacaat tcattgataa cttttctagc gacattatat tgaatgcgaa
    11161 tggacagccg atcccgtttt ccagtcaagt attgagttgc atcatggtga tgctttgggt
    11221 atttgttggt attgaaggag cctctgtcgt aggttcacga gcaaaaaaac aatcagatgt
    11281 tgggaaagca acgattctag ggatattcgg cttgattatt atttacgtct tggtttccat
    11341 gcttccatat gggacaatga cgatgaatca gctgcaaacg atcaaggcgc agcctgctat
    11401 gggctatgtg tttgaaatga tggttggtaa atggggagca gtcgtgatca acggcggttt
    11461 gatcatttct ttagttggtg tttggctctc atggacgatc ttaccgattg aaacaatggg
    11521 aaacatggct gaagacggac ttttgcctaa agcatgggga aaactcaaca aaaatggggc
    11581 accaacttat gctatcgtat tgacgacgtt atgtacgaat gtctttttac tttcattatt
    11641 attcactgac agtgcttata actttgctta cacacttggt acagcagcta ttttcttcac
    11701 gtggcttttc ttaggattgt accaaatgaa actttcttat cagcggaaag aatgggtcca
    11761 atttttagta gggcttttag caagtgcctt tcaagtatgg gctatgctag tcgtagcctt
    11821 taatgaagta atgcttgttc ttgttctttt cttgccaggc atttattttt acctcaaagc
    11881 acgtaaggag caaaacgagc ctgtacaaca tatccactta gataagattt tacttatagg
    11941 tgtgacgatg gtcggagtga ttgcaatggt tttatttgcc actgggatgc ttcacgtata
    12001 gaagtataaa agaagtataa agagaggttg tgagcctgtc gtttattcaa aaacatacta
    12061 ggaaaggagc tgtgacacaa atgttgtcac agctcctttt tcagaataaa cggtgtttaa
    12121 gagacagcca atcggcaata aggctgaacg tctctttcac agcctctttt ttgatgagtc
    12181 atcatacatt ttttcgctta aatacgagat atcctaatgc taagaacaaa acgatgtaag
    12241 ctaaattacc gaatagcatt tgattagtag aaagatgagt caattgcttc atgatctctt
    12301 cgtttccaat ttggttttgc agattcaaca tactgatcgg attccatttg atccactccc
    12361 atttttggat gagtgcaata aggataccag atatgattga cgaagcaaaa taaaagacga
    12421 ttcctgctga gatggatgcg ccggaactat tgataaagca agcaatcatc aacactaaac
    12481 ttaaaatcag ccataaaccg atataactac ccaaagtata agtgaacaag ctttggatca
    12541 gtgtttgacc agtagaaacc ttctccgtga aggaaatcga agggaaaaga atcagtttca
    12601 tcaacacagt gacaataata gctaaaaggt ataaataaac agaataaata actaatgtga
    12661 tccatttgct tactaatatt tctcctcgag aatactttcg ataaaggaga tttttcaatg
    12721 taccatattg tgcttccatc gaaatgatcg tgcttgcagc tgcaatcatg ataaagacaa
    12781 tccaagatgt ggcactaaaa agctgagcag tcatttcaac tggatcaatg atcgtcgtgt
    12841 attttttggc tagaagtgct gttcctatga gcaaaacaac taatagaaca gataaaacag
    12901 cagttgactt tttcttgatc aatttaaata attcttgttt tacaagcaat gtcatttttc
    12961 ttcttccccc tttactgaat ccagaatttc caatagagaa gattccaaat cattcgtcag
    13021 atgctggacg tctgtcacat ggatattttg tgcagacaat agctggatca cggttcctaa
    13081 taattcttcg ttttctttta aaagcttgat tttatcagga tcttccaaag tatatccagc
    13141 ttctgtcaag atcgttcgag cagctagatc atttgttgta gagataacga tgaaatcttg
    13201 cgctgtttgg ttcaattcag ccattgtaca ttgtttgatg atctttcctt gattgatcag
    13261 gatcagctca tcgaccattt tttcaagctc gctgagtaag tgactggaga tcaagaaaga
    13321 ggtgccctga gtagccaagt cggtaatgat ttttcgcaca tctcttgttg cttgtggatc
    13381 caaaccgttc atcggctcat ctaaaataac gatagacgga tggttcagta aggcaagagc
    13441 aattcccaat ttttgtttca ttcccaacga gtaggatttc gcttttttgt gaatgtactt
    13501 ttccatcttg agctgattca cgatagtgtc gatagcagat acatcgttgg tttcagagaa
    13561 taacttcaga tgatcatagc ctgttaagaa tggatagata cccgggtatt cgatcaatgc
    13621 tcctacttgt tccaaccctt tatgactaga gggagaaatc tcattttcac caatacagat
    13681 tgttccatct gagtaatgga tcagtcctaa aatagctttc atgatcgttg tttttccagc
    13741 gccattagga cctactaatc caacgatttt gccaggggat aagctaaaac tgacatcgct
    13801 gagggcttgt ttgtgaccaa agttcttgga taattcttgg accgaaagac ttttcaaaaa
    13861 tattcactcc ttttctatag atagtacaag catacacctg atgagtggag aagtcgctag
    13921 gtcattggac ttatttctta ttttttcata aaaaaattga atattaaata aaaaggctta
    13981 ttttttctga tcagtactta ttccaagtgt ctagtggccg ctagtaatct tgatgccaat
    14041 gatactgatg agtagcatcc ccacaaagaa ccaagtggaa agattcagct tatcatgaaa
    14101 caagaccaca ccaattatga ccgaaccaac tgcgccaatc cctgtccaga taggataaga
    14161 gagactcatc ggcaaacttt tgattgcctt tgacaaaaag taaaaactag caatcatgcc
    14221 aatcactgtg taaaaagaat aattgatttt agaaaaacca tcactccatt tcatcatcgt
    14281 tgaccagaat acttccaaaa ttcctgctat gattaattcc atccatgcca tacttacatt
    14341 cctccttaac tataaaaaaa tacacaaaaa gaggaaaatc atcccttcaa agacctaatg
    14401 gaatgatcat cctctttatc ctacccggtg gcagattgta tatctttttc tgtagtatag
    14461 cacacctatc cagatatcgg caatctcata ctttttagta aagtgtacca agcactgacg
    14521 gatatatcgt tagcggaaaa gaagatgaat gctttgcaat ttcatagaaa gaagttgtta
    14581 tttattaatc atttaaaaaa aacagtaact agaccttatt tctatatatt atcgggcaat
    14641 aaattaaaaa aagctttacc ttacaataag taagataatt tttgtgataa aaaaataacg
    14701 aaaaatattt aaaaatagtg tctatttcgt
//
LOCUS       DAYYFU010000239        13213 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.guided.071, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000239 DAYYFU010000000
VERSION     DAYYFU010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.guided.071"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..2162)
                     /gene="nagE"
                     /locus_tag="ACHA34_003104"
     CDS             complement(204..2162)
                     /gene="nagE"
                     /locus_tag="ACHA34_003104"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM8793635.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="ACHA34_003105"
     CDS             complement(2159..3031)
                     /locus_tag="ACHA34_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8793636.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="ACHA34_003106"
     CDS             complement(3373..4245)
                     /locus_tag="ACHA34_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8793637.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="ACHA34_003107"
     CDS             4373..4942
                     /locus_tag="ACHA34_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM8793638.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="ACHA34_003108"
     CDS             5009..5746
                     /locus_tag="ACHA34_003108"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8793639.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="ACHA34_003109"
     CDS             5977..7245
                     /locus_tag="ACHA34_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8793640.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="ACHA34_003110"
     CDS             7732..8196
                     /locus_tag="ACHA34_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8793641.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="ACHA34_003111"
     CDS             8180..8809
                     /locus_tag="ACHA34_003111"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8793642.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="ACHA34_003112"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="ACHA34_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8793643.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="ACHA34_003113"
     CDS             10688..11134
                     /locus_tag="ACHA34_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8793644.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="ACHA34_003114"
     CDS             complement(11333..11848)
                     /locus_tag="ACHA34_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8793645.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="ACHA34_003115"
     CDS             11965..13167
                     /locus_tag="ACHA34_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8793646.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DAYYFU010000240        11403 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.guided.077, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000240 DAYYFU010000000
VERSION     DAYYFU010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.guided.077"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     misc_binding    25..241
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            289..2934
                     /locus_tag="ACHA34_003116"
     CDS             289..2934
                     /locus_tag="ACHA34_003116"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFM8793647.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            3244..4557
                     /locus_tag="ACHA34_003117"
     CDS             3244..4557
                     /locus_tag="ACHA34_003117"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFM8793648.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            4547..5200
                     /locus_tag="ACHA34_003118"
     CDS             4547..5200
                     /locus_tag="ACHA34_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8793649.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            5255..5806
                     /gene="radC"
                     /locus_tag="ACHA34_003119"
                     /pseudo
     CDS             5255..5806
                     /gene="radC"
                     /locus_tag="ACHA34_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            5937..7022
                     /locus_tag="ACHA34_003120"
     CDS             5937..7022
                     /locus_tag="ACHA34_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8793650.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7019..8911
                     /locus_tag="ACHA34_003121"
     CDS             7019..8911
                     /locus_tag="ACHA34_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFM8793651.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            8918..9295
                     /locus_tag="ACHA34_003122"
     CDS             8918..9295
                     /locus_tag="ACHA34_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HFM8793652.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            9449..10231
                     /locus_tag="ACHA34_003123"
     CDS             9449..10231
                     /locus_tag="ACHA34_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HFM8793653.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(10357..11088)
                     /gene="erm(A)"
                     /locus_tag="ACHA34_003124"
     CDS             complement(10357..11088)
                     /gene="erm(A)"
                     /locus_tag="ACHA34_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HFM8793654.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(11146..11205)
                     /locus_tag="ACHA34_003125"
     CDS             complement(11146..11205)
                     /locus_tag="ACHA34_003125"
                     /inference="COORDINATES: protein motif:HMM:NF019858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HFM8793655.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
BASE COUNT         3929 a         1743 c         2362 g         3369 t
ORIGIN      
        1 aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa ctggcacatt
       61 cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt gaaggtagct
      121 tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta actcacttga
      181 gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat acgtcctaga
      241 atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat gacggaagaa
      301 aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta tcagcattgg
      361 ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta ttcaatcgta
      421 atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga tacgaccttg
      481 caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg gctgcctggt
      541 atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag agagcaaggg
      601 atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga atggaaagaa
      661 gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt agattacagt
      721 agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt ttttgttacc
      781 ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga tcctcaagca
      841 cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc cttttatcat
      901 atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac ccgtccagaa
      961 acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta tcaagcgctc
     1021 atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat tgcagatgaa
     1081 tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca tgatccaaac
     1141 gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga tgatgatggt
     1201 acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc tcgtaaagca
     1261 atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat gatccacagt
     1321 gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac tcaatggttt
     1381 gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga tcaagcagtg
     1441 gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa tgtccatgat
     1501 tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta tcacaaagaa
     1561 acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg ggtacaagat
     1621 cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac aatgggctgg
     1681 ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt agtaaccggt
     1741 tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga atttactggc
     1801 gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca agggcggaag
     1861 atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa gtatggagca
     1921 gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt ccgcttcagc
     1981 tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc ttcacgtttc
     2041 gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg agaaaaatca
     2101 gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt aactgagcta
     2161 tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat ctgggatgat
     2221 ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga tgctgtttca
     2281 aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg tttattgcat
     2341 ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca gggaaattct
     2401 ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac agcttctaaa
     2461 gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc agaagtgaat
     2521 acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa aatcgatcaa
     2581 tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga gttgacgata
     2641 tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg tgcagaaatc
     2701 ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt agaaaaagaa
     2761 ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa tgaacgtttt
     2821 gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa agattattta
     2881 gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca ataagaatag
     2941 aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga acatcgttac
     3001 aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga cataaatttt
     3061 gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg tggatcatgt
     3121 atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt ttctctttgt
     3181 catactttta tttttcttat gtgttctatt tgttataata aaagagaaat gggggaattg
     3241 ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt tggttcacgt
     3301 cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga gagaaaagta
     3361 aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta tttgagaagc
     3421 ctattagaag aggctgggct gacagtaggt acatttactt caccttatat cgaaacgttc
     3481 aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat ccagcttgta
     3541 aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc tggtattact
     3601 gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa agtggacgta
     3661 gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc tgtaccaatg
     3721 ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga tacaattgaa
     3781 gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt aacaggaaat
     3841 attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa tgcaccgctt
     3901 tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat gtggggagaa
     3961 gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc gcttttaggg
     4021 caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta ctgtcaattg
     4081 gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc tcgatggcca
     4141 ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc ccataatacg
     4201 catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata tcaaatcaac
     4261 attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg attgcagcaa
     4321 gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat cgaattaagt
     4381 gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca attcggatta
     4441 gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg ttcactttat
     4501 ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga agtataatgc
     4561 agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt acgaagccac
     4621 acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt taaaattctt
     4681 aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg aatttggcaa
     4741 aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac gtttttcgca
     4801 aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag aagaacatca
     4861 gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt tattgaagaa
     4921 gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc aacgagcaaa
     4981 accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga agaaagaaac
     5041 attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg agatcccggt
     5101 agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa cagctggggt
     5161 tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct cttttcgtat
     5221 cttattttag ataagataga aaggagatgt ttttttggga aaacgattga tcaaagaagt
     5281 acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag ctttatcgga
     5341 tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg tgatgtcgat
     5401 tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg ctactttgca
     5461 tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca aagccttgat
     5521 cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg ttcgtaccag
     5581 ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag aacatttggt
     5641 ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt tcatcggttc
     5701 gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac gttattgtgc
     5761 agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttttaacca tagtataata
     5821 aataatagta gttaaagtag tttaagagga gatattaaag ggatatttta ctttaaataa
     5881 aaattaacaa ggtttataat ttgtagtagt taaagtattt aaagaggtgg gaacatgtga
     5941 aggttcaaag gatagaagtg gagaataagc cgtatccatt gtatttatta ctagataaag
     6001 aataccagct aatagaacca gtaatgaaat ttattaaata cttagataat actggtaagt
     6061 ctcctaatac cattaaagca tactgctatc atttaaagtt gctgtacgag ttcatggaac
     6121 agagaggtgt tattcttaat gatattaact ttgagttgtt agcagacttc gtaggttggt
     6181 tgagatatcc ttcagcatca aatgtaattg atcttcagtc aaaaaaagcc ataagagaag
     6241 aaacgacagt gaatacaatt ttaaatgtag ttatgagttt tcttgattat ttaagtagat
     6301 taggagaatt taaatcaatt gatgtattta aacaagcaaa gggaagaaat ttcaaaggat
     6361 ttttacatca tgttaataag ggtagatacc aaaagaatgt cttaaagtta agggttaaaa
     6421 agaaacagat aagaacattg agatcagagg aagttaagca aattattgat gcttgtcata
     6481 cgaaaagaga taaattaatt ttaatgctta tgtatgaggg tggtttaaga atcggtgaag
     6541 tgttatcgct taggcttgaa gatattgtca cttgggacaa tcaaatccat ttaacaccta
     6601 gagatgttaa tgttaatgaa gcttatatta aattaaggaa ggaaagaaca atacatgtga
     6661 gtaaagaact tatgtcactt tatacagatt acttgatata tgagtatagt gaggaattgg
     6721 agcatgatta tgtttttatt tccttaaaag aaggctattt tgggaaacca ctaaagtacc
     6781 aaagtgttct tgatctagtt agaagaatag ttaaaaggac tggaatagaa tttacatcac
     6841 atatgcttcg ccacactcac gcaacgcagc taattaggga aggatgggat gttgcgttcg
     6901 ttcaaaagag attaggtcac gcacatgttc agacaacgtt aaatacctat gttcatcttt
     6961 cagatcagga tatgaaaaat gagtttaata aatacctcga gagaaaggag cataagaaat
     7021 gaatgcttct agtaaaagga aaattattag tcagagtgag attagcaaaa aaatagctgt
     7081 aatgaatgaa gaaatgcagg ggttttgggc taataatagt tgggatataa gaaaatgtcc
     7141 acatccttct gccatagaat taagtaagaa tcctgcttta aggaatcgtt gggttcgttt
     7201 tgaacgtgtt aaaaatctgt ggttaagaac agaattgaaa tatttttatt tttaccattt
     7261 aaacaatgga atatggaatg caaaaactgt ctggattaga aaaggaacag taattaataa
     7321 aatgttagat ttcttagatt taaagtatcc tagcattact tcaattactg aagttcctat
     7381 tgaaaaagca atgacggagt atagaactta tttgacaaaa cggggtgtta gaattaccac
     7441 tactaattat aagattactg ctaatcaaga aaaaacacct gtaaaagcta attcctacta
     7501 tgttactaat ctaaaacaat ttatggagtt ttatgagaac ttttattttg atggagagga
     7561 gtgggataaa gacgtttggg atagacgtaa cttacctttg ccagatgata aggttaaccc
     7621 aacacaatat gaatatacaa ttaactttaa agggtttcgg aatacatatt ttaaacaact
     7681 tgtaaaaaga tattgtaagt tgagattgaa catggatagc ttttcctatg taagtgatat
     7741 tgcccaaaga cttaaagagt tctttaattt tctggacatg aaatttaaac aagttcagag
     7801 agtacaccaa ttaacgagag tggaaattga agcatattta agtgaactaa acatgatggg
     7861 aataaaacct agtacaataa ctgggaggat ctctatattg gaaggactat ttagtaccct
     7921 tcttaggcta gaatgggatg atgttccttc caaaatatta atttattctg aggactatcc
     7981 gaaaatacca agagcaaaac cacgctttat agatgaattc gtcctagagc aattgaacag
     8041 tcatcttgat aaattacccg aatatatagc tacgatgact atgattgttc aagaatgtgg
     8101 aatgaggata agtgaattgt gcaccttgaa aaaaggctgt ctattagagg acaaagatgg
     8161 agatttcttt ttaaagtatt atcaatggaa aatgaaaaag gagcatatag ttccaatatc
     8221 taaagaggta gctttactta ttaaagttcg ggaagataaa gtttcagagg aatttccaga
     8281 tagtgaatac ctctttccaa gaaaagatgg atcgccatta aaacaagaaa catttagagg
     8341 tgagttaaat aaattagctt atgagcaaaa tatagtggat aaatcaggtg agatttatag
     8401 attccatgcc catgcctttc gccatacagt aggaacaaga atgattaaca acgggatgcc
     8461 ccagcatatt gtgcagaaat ttttggggca tgaaagccca gaaatgacaa gcagatacgc
     8521 tcatatcttt gatgaaactc taaaaaatga atttactaaa tttcaggaaa aactggttac
     8581 caataatgga gatgtgcttg atctagatga agataatgaa gtcgatgatg tagagcttca
     8641 atggttcaag aaaaatataa atgcacaagt gcttccaaat ggttattgta gattgccagt
     8701 agtagcaggt ggttgtccac atgcgaatgc atgcttagat tgcactcact tctgtaccag
     8761 taagcaattc ttaccacagc acgaagaaca gttagagcgt acagaagagt tattagccat
     8821 agctaaggat aaacaatggc aaagacaagt agagactaat agccgtgtta aagagcgttt
     8881 agaacaaatc attggaagtt tgacggggta attatcaatg gataaacaag ttagaaatac
     8941 aacagaaatt gtacgtttgg cgaagcagaa atcaaaaaag acaagggaaa aagtagacaa
     9001 agcgatttct aaattttcga ttgaaggtaa agttattaat tttaattcaa tagcaaagga
     9061 agctaatgtt tctaaatcat ggctttataa ggaacacgat attaggcaaa gaatcgaatc
     9121 ccttcgtgag cgtcaaataa cagcaaatgt agtctcaaaa cccaagaaaa gttctcgttc
     9181 ggaggaaatc cttattaaaa ccttaaaaag aagagtaatg gaattagaaa aagaaaataa
     9241 aaaattacag aaccaaattc aaaaattata tggagatctg tataataaag aataattatt
     9301 aatctgtaga caaattgtga aaggatgtac ttaaacgcta acggtcagct ttattgaaca
     9361 gtcatttagg tatatgtcca atctagggta agtaaattga gtatcaatat aaactttata
     9421 tgaacataat cagttacgag gtgaaatcat gagcaatttg attaacggaa aaataccaaa
     9481 tcaagcgatt caaacattaa aaatcgtaaa agatttattt ggaagttcaa tagttggagt
     9541 atatctattt ggttcagcag taaatggtgg tttacgcatt aacagcgatg tagatgttct
     9601 agtcgtcgtg aatcatagtt tacctcaatt aactcgaaaa aaactaacag aaagactaat
     9661 gactatatca ggaaagattg gaaatacgga ttctgttaga ccacttgaag ttacggttat
     9721 aaataggagt gaagttgtcc cttggcaata tcctccaaaa agagaattta tatacggtga
     9781 gtggctcagg ggtgaatttg agaatggaca aattcaggaa ccaagctatg atcctgattt
     9841 ggctattgtt ttagcacaag caagaaagaa tagtatttct ctatttggtc ctgattcttc
     9901 aagtatactt gtctccgtac ctttgacaga tattcgaaga gcaattaagg attctttgcc
     9961 agaactaatt gaggggataa aaggtgatga gcgtaatgta attttaaccc tagctcgaat
    10021 gtggcaaaca gtgactactg gtgaaattac ctcgaaagat gtcgctgcag aatgggctat
    10081 acctctttta cctaaagagc atgtaacttt actggatata gctagaaaag gctatcgggg
    10141 agagtgtgat gataagtggg aaggactata ttcaaaggtg aaagcactcg ttaagtatat
    10201 gaaaaattct atagaaactt ctctcaatta ggctaatttt attgcaataa caggtgctta
    10261 cttttaaaac tactgattta ttgataaata ttgaacaatt tttgggaaga ataaagcgtc
    10321 ctcttgtgaa attagagaac gctttattac tttaatttag tgaaacaatt tgtaactatt
    10381 gaaaatagaa agaaattgtt ccttcgatag tttattaata ttagtgacat ttgcatgctt
    10441 caaagcctgt cggaattggt ttttagtgaa aagaacacga tattcacggt ttacccactt
    10501 ataaacaaaa gatcgatact ttttgtagtc cttctttgaa atcaatggtt gatgtcgttc
    10561 aagaacaatc aatacagagt ctacacttgg cttaggatga aaatatagtg gtggtacttt
    10621 tttgagcatt tttatatcca tctccaccat taatagtaaa cccaaagctc gttgcagatt
    10681 ttgcaatctt ttcgcaaatc ccttctcaac gataagatag ctatatttag cctgactttc
    10741 aaaggtaatt cttttgacaa tatccgtact gatgttataa ggaatattac catatatctt
    10801 atagtttata tgttttggga aggaaaattt tagaatatcc gtttgaatca cttttatatt
    10861 ctcagagggg tttaccgctt ctttagtcac ttgacataag cctccatcaa tttctatagc
    10921 agtaactgat cgactcattt tgactagctc tttggtaaaa tgtccttttc ctgatccgat
    10981 ttctattacg ttgtcttgtt tactgatatt cgtgtgattc aatatttctt ttacatgctt
    11041 tttagaagta ataaaatttt gcgtgtcttt agggtttttc tggttcatta taaccttctc
    11101 cttactggtt ataatgaact gacattaagc gttcattata accaattaat tttgatttgg
    11161 ttgataacga actttattaa taacaaatat agaaaaagta cccatacttt tatctccttt
    11221 ccattttttt cattatgaat gagataaagt aatttctact actgcgatac tggtgcacat
    11281 aattgaaacc tccttatgta actgaatatg attgtatcat cttttcattt tagaataaac
    11341 ctttatatgt aattttgcac ttgacacttc agatggacgt tttcttcaaa ataatatgtt
    11401 ttg
//
LOCUS       DAYYFU010000241         5613 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.guided.112, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000241 DAYYFU010000000
VERSION     DAYYFU010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.guided.112"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(40..1026)
                     /locus_tag="ACHA34_003126"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="ACHA34_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR-A"
                     /locus_tag="ACHA34_003127"
     CDS             1178..1873
                     /gene="vanR-A"
                     /locus_tag="ACHA34_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFM8793656.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..3005
                     /locus_tag="ACHA34_003128"
     CDS             1851..3005
                     /locus_tag="ACHA34_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFM8793657.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3220..4188
                     /locus_tag="ACHA34_003129"
     CDS             3220..4188
                     /locus_tag="ACHA34_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFM8793658.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4181..5212
                     /gene="vanA"
                     /locus_tag="ACHA34_003130"
     CDS             4181..5212
                     /gene="vanA"
                     /locus_tag="ACHA34_003130"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFM8793659.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5218..5583
                     /locus_tag="ACHA34_003131"
                     /pseudo
     CDS             5218..5583
                     /locus_tag="ACHA34_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
BASE COUNT         1697 a         1091 c         1262 g         1563 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agttctatag gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg
     2881 cgattgcaaa agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata
     2941 actatacgac gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt
     3001 cctaagagat gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg
     3061 aaattaacaa tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa
     3121 gaacgagccg ttttcgttct tcagagaaag atttgacaag attaccattg gcatccccgt
     3181 tttatttggt gcctttcaca gaaagggttg gtcttaatta tgaataacat cggcattact
     3241 gtttatggat gtgagcagga tgaggcagat gcattccatg ctctttcgcc tcgctttggc
     3301 gttatggcaa cgataattaa cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc
     3361 aatcaatgta tcagtgtggg acataaatca gagatttccg cctctattct tcttgcgctg
     3421 aagagagccg gtgtgaaata tatttctacc cgaagcatcg gctgcaatca tatagataca
     3481 actgctgcta agagaatggg catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt
     3541 gccgattata ctatgatgct aattcttatg gcagtacgca acgtaaaatc gattgtgcgc
     3601 tctgtggaaa aacatgattt caggttggac agcgaccgtg gcaaggtact cagcgacatg
     3661 acagttggtg tggtgggaac gggccagata ggcaaagcgg ttattgagcg gctgcgagga
     3721 tttggatgta aagtgttggc ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg
     3781 tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct caatacggat
     3841 acgcactata ttatcagcca cgaacaaata cagagaatga agcaaggagc atttcttatc
     3901 aatactgggc gcggtccact tgtagatacc tatgagttgg ttaaagcatt agaaaacggg
     3961 aaactgggcg gtgccgcatt ggatgtattg gaaggagagg aagagttttt ctactctgat
     4021 tgcacccaaa aaccaattga taatcaattt ttacttaaac ttcaaagaat gcctaacgtg
     4081 ataatcacac cgcatacggc ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa
     4141 accattaaaa actgtttgga ttttgaaagg agacaggagc atgaatagaa taaaagttgc
     4201 aatactgttt gggggttgct cagaggagca tgacgtatcg gtaaaatctg caatagagat
     4261 agccgctaac attaataaag aaaaatacga gccgttatac attggaatta cgaaatctgg
     4321 tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt gctattcagc
     4381 tgtactctcg ccggataaaa aaatgcacgg attacttgtt aaaaagaacc atgaatatga
     4441 aatcaaccat gttgatgtag cattttcagc tttgcatggc aagtcaggtg aagatggatc
     4501 catacaaggt ctgtttgaat tgtccggtat cccttttgta ggctgcgata ttcaaagctc
     4561 agcaatttgt atggacaaat cgttgacata catcgttgcg aaaaatgctg ggatagctac
     4621 tcccgccttt tgggttatta ataaagatga taggccggtg gcagctacgt ttacctatcc
     4681 tgtttttgtt aagccggcgc gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc
     4741 ggacgaattg gactacgcaa ttgaatcggc aagacaatat gacagcaaaa tcttaattga
     4801 gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg ccgcgttagt
     4861 tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc atcaggaagt
     4921 cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc tttcagcaga
     4981 ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg
     5041 tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga acgaagtcaa
     5101 tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg ctgcaggtat
     5161 tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt gataagcatg
     5221 gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga cgctaaatat
     5281 gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta
     5341 gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc tacccaaggg
     5401 tacggattgc ttctatggga cggttaccgt cctaagcgtg ctgtaaactg ttttatgcaa
     5461 tgggctgcac agccggaaaa taacctgaca aaggaaagtt attatcccaa tattgaccga
     5521 actgagatga tttcaaaagg atacgtggct tcaaaatcaa gccatagccg cggcggttct
     5581 gttgcaaagt tttaaataaa gaataaaatc cct
//
LOCUS       DAYYFU010000242         1781 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.guided.170, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000242 DAYYFU010000000
VERSION     DAYYFU010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.guided.170"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..416
                     /locus_tag="ACHA34_003132"
     CDS             24..416
                     /locus_tag="ACHA34_003132"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8793660.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="ACHA34_003133"
     CDS             417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="ACHA34_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HFM8793661.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          731 a          136 c          303 g          611 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggttatgca aaagaaaaag
     1141 caatatataa ttttttaaat acaaatttag aaactaatgt aaaaattcct aatattgaat
     1201 attcgtatat tagtgatgaa ttatctatac taggttataa agaaattaaa ggaacttttt
     1261 taacaccaga aatttattct actatgtcag aagaagaaca aaatttgtta aaacgagata
     1321 ttgccagttt tttaagacaa atgcacggtt tagattatac agatattagt gaatgtacta
     1381 ttgataataa acaaaatgta ttagaagagt atatattgtt gcgtgaaact atttataatg
     1441 atttaactga tatagaaaaa gattatatag aaagttttat ggaaagacta aatgcaacaa
     1501 cagtttttga gggtaaaaag tgtttatgcc ataatgattt tagttgtaat catctattgt
     1561 tagatggcaa taatagatta actggaataa ttgattttgg agattctgga attatagatg
     1621 aatattgtga ttttatatac ttacttgaag atagtgaaga agaaatagga acaaattttg
     1681 gagaagatat attaagaatg tatggaaata tagatattga gaaagcaaaa gaatatcaag
     1741 atatagttga agaatattat cctattgaaa ctattgttta t
//
LOCUS       DAYYFU010000243         1504 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.guided.176, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000243 DAYYFU010000000
VERSION     DAYYFU010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.guided.176"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            262..345
                     /locus_tag="ACHA34_003134"
                     /pseudo
     CDS             262..345
                     /locus_tag="ACHA34_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            394..477
                     /locus_tag="ACHA34_003135"
     CDS             394..477
                     /locus_tag="ACHA34_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFM8793662.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="ACHA34_003136"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="ACHA34_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8793663.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DAYYFU010000244         1382 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.guided.181, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000244 DAYYFU010000000
VERSION     DAYYFU010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.guided.181"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1311
                     /locus_tag="ACHA34_003137"
     CDS             <1..1311
                     /locus_tag="ACHA34_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HFM8793664.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          584 a          108 c          234 g          456 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     1321 atatgaaaag ctattataaa agacggttct gttgcaaagt tttaaatcta ctatcaaata
     1381 ag
//
LOCUS       DAYYFU010000245         1350 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.guided.183, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000245 DAYYFU010000000
VERSION     DAYYFU010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.guided.183"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..298
                     /locus_tag="ACHA34_003138"
                     /pseudo
     CDS             47..298
                     /locus_tag="ACHA34_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="ACHA34_003139"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="ACHA34_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFM8793665.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DAYYFU010000246         1194 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.guided.188, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000246 DAYYFU010000000
VERSION     DAYYFU010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.guided.188"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            259..>1194
                     /gene="vanY"
                     /locus_tag="ACHA34_003140"
     CDS             259..>1194
                     /gene="vanY"
                     /locus_tag="ACHA34_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFM8793666.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          426 a          173 c          238 g          357 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattg gttctgttgc aaagttttaa atctactatc aaat
//
LOCUS       DAYYFU010000247         1074 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5153
            SAMEA115707532-rid22595703.guided.197, whole genome shotgun
            sequence.
ACCESSION   DAYYFU010000247 DAYYFU010000000
VERSION     DAYYFU010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707532
            Sequence Read Archive: ERR13197261
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290108.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290108.1
            Genome Coverage       :: 178x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:16:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,141
            CDSs (total)                      :: 3,077
            Genes (coding)                    :: 2,980
            CDSs (with protein)               :: 2,980
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 30 of 97
            Pseudo Genes (incomplete)         :: 55 of 97
            Pseudo Genes (internal stop)      :: 27 of 97
            Pseudo Genes (multiple problems)  :: 12 of 97
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707532-rid22595703.guided.197"
                     /isolate="VRE5153"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            359..907
                     /gene="aac(6')"
                     /locus_tag="ACHA34_003141"
     CDS             359..907
                     /gene="aac(6')"
                     /locus_tag="ACHA34_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8793667.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          371 a          157 c          214 g          332 t
ORIGIN      
        1 tttttgttcc tcttagtatt tctaccgatt ccctttgctt ttttgtaatt atctaaaaat
       61 tctaaaaatt aagattatta tatgaaaaca ggttttattt gtcaattcat aattggtttt
      121 ttttaaagca aaagataatc tatatcatga tgaacaaaca gagttttttc ctccaattga
      181 tggctttcct gatctttcct attttgaata aagtgtttct gtataatttt tttattttat
      241 aagtaagata aataatgaaa gaatagagat actgattggt agctaaagat ttttgagcga
      301 gaacatttcg ttttttcaaa aggactgtta gaataagcaa gaacaagggg gaagacgtat
      361 gataatcagt gaatttgacc gtaataatcc agtattgaaa gatcagcttt ctgatttact
      421 gagactgact tggccggaag aatatggaga cagctcggca gaagaagtag aagaaatgat
      481 gaatccagaa cgaatcgcgg tagcagcggt agaccaagat gagttagtag gatttattgg
      541 tgcaatccct caatacggta tcacaggttg ggaattgcat ccattagttg tagaaagctc
      601 ccgacgaaag aaccaaatag gtactcgatt agtcaattac ttagaaaaag aagtagcttc
      661 cagaggagga atcacgattt atttaggtac ggatgattta gaccatggaa caacgttaag
      721 tcaaacggac ctgtatgagc atacatttga taaagtggct tccatccaga accttcgtga
      781 acatccgtat gaattctatg aaaaattagg ttataaaatc gtaggtgtct taccaaatgc
      841 aaatggctgg gacaaaccgg atatttggat ggcaaaaacg attattcctc gaccaaattc
      901 tcaataataa aaagaggttg tgacagaagt aatcagcttc aagaaataag aaggaattta
      961 cgaaaattac ttcttgaatt tttgtgaaat tccaacttat ttccgaagaa gctacttttg
     1021 gaacaccgtg taccagaggt tgtgaaaaag tcgcttagtt tcgagtagta agaa
//
